title: Discovery of a novel HLA-DRB1*04:05 variant, DRB1*04:05:15, in a Taiwanese and the probable HLA haplotype in association with DRB1*04:05:15.
abstract: Here, we report a novel HLA-DRB1*04 allele, DRB1*04:05:15, found in a Taiwanese unrelated volunteer bone marrow hematopoietic stem cell donor by a sequence-based typing (SBT) method. The DNA sequence of DRB1*04:05:15 is identical to the sequence of DRB1*04:05:01 in exon 2, except the nucleotide at the position 198 where C is substituted by T (TAC→TAT at codon 37). Due to the silent mutation, the nucleotide replacement generated no amino acid variation in comparison with DRB1*04:05:01. We postulate the allele DRB1*04:05:15 was probably derived from DRB1*04:05:01 via a nucleotide point mutation event. The probable HLA-A, -B, -C, -DRB1 and -DQB1 haplotype in association with DRB1*04:05:15 may be deduced as A*02:01-B*48:01-C*08:03-DRB1*04:05:15-DQB1*04:01.
authors.affiliation: Laboratory of Immunogenetics and Cord Blood Bank, Buddhist Tzu Chi General Hospital, Buddhist Tzu Chi Stem Cells Centre and Marrow Donor Registry, Buddhist Compassion Relief Tzu Chi Foundation, Hualien, Taiwan. edward@tzuchi.com.tw
authors.forename: K L
authors.initials: KL
authors.lastname: Yang
authors.forename: S K
authors.initials: SK
authors.lastname: Lee
authors.forename: P Y
authors.initials: PY
authors.lastname: Lin
mesh_terms: D044466:Asian People / Q000235:genetics*; D001483:Base Sequence; D001853:Bone Marrow; D014644:Genetic Variation / Q000235:genetics; D059811:HLA-DRB1 Chains / Q000235:genetics* / Q000302:isolation & purification; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D017354:Point Mutation; D017422:Sequence Analysis, DNA; D013624:Taiwan
chemical_list: D059811:HLA-DRB1 Chains; C493960:HLA-DRB1*04:05 antigen