title: A novel method for measuring intestinal and hepatic triacylglycerol kinetics.
abstract: This study aimed to 1) develop a method that completely separated hepatic (VLDL1, VLDL2) and intestinal [chylomicron (CM)] lipoproteins and 2) use the method to measure triacylglycerol (TAG) kinetics in these lipoproteins in the fed and fasting state in healthy subjects, using intravenous [²H₅]glycerol as the tracer. An immunoaffinity method that completely separated hepatic and intestinal particles using sequential binding to three antibodies to apolipoprotein B-100 (apoB-100) was established and validated. Six healthy volunteers were studied in a fasted and continuous feeding study (study 1). Five additional healthy volunteers were studied in a continuous feeding study that included an oral [¹³C₃]glycerol tripalmitin tracer (study 2). In both studies, an intravenous bolus of [²H₅]glycerol was administered to label TAG in hepatic and intestinal lipoproteins. In both feeding studies there was sufficient incorporation of the [²H₅]glycerol tracer into the exogenous lipoproteins to enable isotopic enrichment to be measured. In study 2, the oral tracer enrichment in VLDL1 was <5% of CM enrichment 150 min after tracer administration, demonstrating negligible contamination of VLDL1 with apoB-48. Western blotting showed no detectable apoB-100 in CMs. VLDL1 and VLDL2 TAG fractional catabolic rate (FCR) did not differ between feeding and fasting (study 1). There was no difference between CM and VLDL1 TAG FCR in both fed studies. In fed study 2, 47% of the total TAG production rate (CM + VLDL1) was from CM. This methodology may be a useful tool for understanding the abnormalities in postprandial TAG kinetics in metabolic syndrome and type 2 diabetes.
authors.affiliation: Diabetes and Metabolic Medicine, Faculty of Health and Medical Sciences, University of Surrey, Guildford, United Kingdom; and.
authors.forename: Feifei
authors.initials: F
authors.lastname: Sun
authors.forename: Mike
authors.initials: M
authors.lastname: Stolinski
authors.forename: Fariba
authors.initials: F
authors.lastname: Shojaee-Moradie
authors.forename: Shaoying
authors.initials: S
authors.lastname: Lou
authors.forename: Yuying
authors.initials: Y
authors.lastname: Ma
authors.forename: Roman
authors.initials: R
authors.lastname: Hovorka
authors.forename: A Margot
authors.initials: AM
authors.lastname: Umpleby
mesh_terms: D002247:Carbon Isotopes; D002846:Chromatography, Affinity; D002914:Chylomicrons / Q000302:isolation & purification / Q000378:metabolism*; D003903:Deuterium; D005215:Fasting; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D007700:Kinetics; D008079:Lipoproteins, VLDL / Q000302:isolation & purification / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D019518:Postprandial Period; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D002247:Carbon Isotopes; D002914:Chylomicrons; D008079:Lipoproteins, VLDL; D014280:Triglycerides; D003903:Deuterium
keywords: chylomicron; immunoaffinity; postprandial; stable isotopes; very-low-density lipoprotein