title: The pathology of enterotoxemia by Clostridium perfringens type C in calves.
abstract: The pathology of Clostridium perfringens type C infection has been described with detail only in foals and piglets. The current report describes the diagnostic workup and detailed pathology of 3 cases of C. perfringens type C infection in calves. A 2-day-old Jersey calf and fresh and fixed tissues from a 4-week-old Angus calf and from a 1-week-old Jersey calf were received at the California Animal Health and Food Safety Laboratory System with a history of digestive disease and death. The gross changes in the gastrointestinal tract of 1 calf consisted of multifocal subserosal hemorrhages of the rumen, diffuse congestion and multifocal hemorrhages of the small intestinal mucosa, and dilated cecum with bloody liquid contents. In a second calf, a large segment of small intestine was hemorrhagic. The small intestine of the third calf was dilated and filled with abundant yellow fluid content. Microscopically, all 3 calves had diffuse coagulation necrosis of the intestinal mucosa. Clostridium perfringens type A was isolated from the intestinal content of 2 calves. In addition, enzyme-linked immunosorbent assay for Bovine rotavirus was positive on colonic content of 1 calf. Small numbers of cryptosporidia were seen in smears of colonic content of 2 calves, and Salmonella sp. group E was detected in the small intestinal content of another calf. Clostridium perfringens beta toxin was detected in the intestinal content of the 3 animals. A diagnosis of C. perfringens type C infection was confirmed based on pathological findings and detection of beta toxin in the intestinal content of the 3 animals.
authors.affiliation: California Animal Health and Food Safety Laboratory, San Bernardino Branch, 105 West Central Avenue, San Bernardino, CA 92408, USA.
authors.forename: Jorge P
authors.initials: JP
authors.lastname: Garcia
authors.forename: Mark
authors.initials: M
authors.lastname: Anderson
authors.forename: Patricia
authors.initials: P
authors.lastname: Blanchard
authors.forename: Asli
authors.initials: A
authors.lastname: Mete
authors.forename: Francisco A
authors.initials: FA
authors.lastname: Uzal
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000382:microbiology* / Q000473:pathology; D003016:Clostridium perfringens / Q000302:isolation & purification*; D004767:Enterotoxemia / Q000382:microbiology*
keywords: Calves; Clostridium perfringens type C; enterotoxemia