title: Fluorescence microscopy-a historical and technical perspective.
abstract: For a little more than a century, fluorescence microscopy has been an essential source of major discoveries in cell biology. Recent developments improved both visualization and quantification by fluorescence microscopy imaging and established a methodology of fluorescence microscopy. By outlining basic principles and their historical development, I seek to provide insight into and understanding of the ever-growing tools of fluorescence microscopy. Thereby, this synopsis may help the interested researcher to choose a fluorescence microscopic method capable of addressing a specific scientific question.
authors.affiliation: Department of Obstetrics & Gynecology and Women's Health, Albert Einstein College of Medicine, Bronx, New York 10461; Eunice Kennedy Shriver Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892.
authors.forename: Malte
authors.initials: M
authors.lastname: Renz
mesh_terms: D003952:Diagnostic Imaging; D005453:Fluorescence; D005456:Fluorescent Dyes; D049672:History, 19th Century; D049673:History, 20th Century; D049674:History, 21st Century; D008856:Microscopy, Fluorescence / Q000266:history / Q000379:methods* / Q000639:trends*
chemical_list: D005456:Fluorescent Dyes
keywords: diffraction-limited microscopy; fluorescence microscopy; historical perspective; super-resolution microscopy