title: Relationship between post-SARS osteonecrosis and PAI-1 4G/5G gene polymorphisms.
abstract: OBJECTIVE
  To explore the correlation between post-severe acute respiratory symptom (SARS) patients with osteonecrosis, investigate the etiology of post-SARS osteonecrosis and select the sensitive molecular symbols for early diagnosis and distinguish the high-risk population.
  
  
  METHODS
  The studied subjects were divided into two groups. Sixty-two post-SARS patients with osteonecrosis were one group, and 52 age- and sex-matched healthy people were as normal controlled group. Empty stomach blood samples from cubital veins were collected from both groups. Plasminogen activator inhibitor (PAI) by means of enzyme-linked immunosorbent assay and PAI-1 4G/5G polymorphism was detected by polymerase chain reaction and solid phase oligonucleotide assay.
  
  
  RESULTS
  The blood agents of post-SARS patients changed obviously with 15.64 ± 13.85 U/ml while the control group 7.96 ± 4.27 U/ml; 4G/4G genotype for the PAI-1 polymorphism detected in post-SARS group was more than that of the control group, but had no statistical significance. The plasma PAI activity was related to homozygote 4G/4G genotype. This reveals that homozygote 4G/4G genotype may be a susceptible gene mark to Chinese osteonecrosis patients.
  
  
  CONCLUSIONS
  Plasminogen activator inhibitor-1 is sensitive blood symbol for screening high-risk susceptible population; 4G/4G PAI-1 genotype may be an etiological factor in osteonecrosis.
authors.affiliation: Department of Orthopaedic Surgery, Center for Osteonecrosis and Joint Preserving and Reconstruction, China-Japan Friendship Hospital, Beijing, 100029, China, Sun887@163.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Sun
authors.forename: Zirong
authors.initials: Z
authors.lastname: Li
authors.forename: Zhengcai
authors.initials: Z
authors.lastname: Shi
authors.forename: Bailiang
authors.initials: B
authors.lastname: Wang
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Gao
authors.forename: Yurun
authors.initials: Y
authors.lastname: Yang
authors.forename: Wanshou
authors.initials: W
authors.lastname: Guo
mesh_terms: D000293:Adolescent; D000328:Adult; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006720:Homozygote; D006801:Humans; D008297:Male; D008875:Middle Aged; D010020:Osteonecrosis / Q000235:genetics* / Q000821:virology*; D017395:Plasminogen Activator Inhibitor 1 / Q000235:genetics*; D011110:Polymorphism, Genetic; D045169:Severe Acute Respiratory Syndrome / Q000150:complications*; D055815:Young Adult
chemical_list: D017395:Plasminogen Activator Inhibitor 1; C051321:SERPINE1 protein, human