title: Evaluation of three-dimensional gel electrophoresis to improve quantitative profiling of complex proteomes.
abstract: Two-dimensional remains one of the main experimental approaches in proteome analysis. However, comigration of protein leads to several limitations: lack of accuracy in protein identification, impaired comparative quantification, and PTM detection. We have optimized a third additional step of in-gel separation to alleviate comigration associated drawbacks. Spot resolution is strikingly improved following this simple and rapid method and the positive impact on protein and peptide identification from MS/MS data, on the analysis of relative changes in protein abundance, and on the detection of PTM is described.
authors.affiliation: Département Sciences du Vivant, Centre wallon de Recherches agronomiques, Gembloux, Belgium.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Colignon
authors.forename: Martine
authors.initials: M
authors.lastname: Raes
authors.forename: Marc
authors.initials: M
authors.lastname: Dieu
authors.forename: Edouard
authors.initials: E
authors.lastname: Delaive
authors.forename: Sergio
authors.initials: S
authors.lastname: Mauro
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D010940:Plant Proteins / Q000032:analysis / Q000302:isolation & purification; D011499:Protein Processing, Post-Translational; D020543:Proteome / Q000032:analysis* / Q000737:chemistry*; D040901:Proteomics / Q000379:methods*; D011198:Solanum tuberosum / Q000737:chemistry
chemical_list: D010936:Plant Extracts; D010940:Plant Proteins; D020543:Proteome
keywords: 3DE; Comigration; DIGE; PTM; Protein identification; Technology