title: Biological characterization of oxidized hyaluronic acid/resveratrol hydrogel for cartilage tissue engineering.
abstract: Osteoarthritis is a type of arthritis that is caused by breakdown of cartilage, with eventual loss of the cartilage of the joints. The ability of self-repair in damaged cartilage tissue is limited; the aim of this work is to fabricate and characterize an oxidized hyaluronic acid/resveratrol (Oxi-HA/Res) hydrogel for future applications in cartilage tissue engineering. Under physiological conditions, the Oxi-HA/Res hydrogel was prepared by chemical crosslinking of Oxi-HA with resveratrol solution and characterized by Fourier transform infrared spectrometry assay; the biocompatibility and gene expression of chondrocytes within the Oxi-HA-Res hydrogel then analyzed. The cell viability and cytotoxicity assays showed that the Oxi-HA/Res hydrogel has good biocompatibility. Oxi-HA/Res hydrogel can upregulate expression of type II collagen, aggrecan, and Sox-9 genes; while down-regulating IL-1β, MMP-1, MMP-3, MMP13 gene expression. It can also reduce LPS-induced inflammation and chondrocyte damage. The results of this study showed that the Oxi-HA/Res hydrogel is biocompatible with chondrocytes, allows for extracellular matrix synthesis, and also reduce LPS-induced inflammation and damage. These results suggest that Oxi-HA/Res hydrogel may be a potential suitable cell carrier for chondrocyte cells in the treatment of cartilage defect. However, further in vivo study is mandatory for future possible clinical applications.
authors.affiliation: School of Pharmacy, College of Pharmacy, Taipei Medical University, No. 250, Wusing St., Sinyi District, Taipei City 11031, Taiwan, Republic of China.
authors.forename: Shiow-Yunn
authors.initials: SY
authors.lastname: Sheu
authors.forename: Wen-Shan
authors.initials: WS
authors.lastname: Chen
authors.forename: Jui-Sheng
authors.initials: JS
authors.lastname: Sun
authors.forename: Feng-Huei
authors.initials: FH
authors.lastname: Lin
authors.forename: Tuoh
authors.initials: T
authors.lastname: Wu
mesh_terms: D000818:Animals; D002356:Cartilage / Q000187:drug effects* / Q000502:physiology*; D019902:Chondrocytes / Q000187:drug effects / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D006820:Hyaluronic Acid / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000494:pharmacology*; D051379:Mice; D008813:Mice, Inbred ICR; D008855:Microscopy, Electron, Scanning; D010084:Oxidation-Reduction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000077185:Resveratrol; D017550:Spectroscopy, Fourier Transform Infrared; D013267:Stilbenes / Q000494:pharmacology*; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D003432:Cross-Linking Reagents; D012333:RNA, Messenger; D013267:Stilbenes; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D006820:Hyaluronic Acid; D000077185:Resveratrol
keywords: cartilage tissue engineering; hyaluronic acid; injectable; resveratrol