title: Novel PCR assay for determining the genetic sex of mice.
abstract: A number of studies require the determination of the genetic sex of mouse embryos before sexual differentiation and/or of mutant mice that display partial or complete sex reversal. The majority of current methods for sexing by PCR involve multiplexing of 2 primer pairs. We have developed a novel sexing PCR using a single primer pair that amplifies fragments from the X and the Y chromosome with a clear size difference between the respective amplicons. This assay provides a rapid and reliable method to identify the genetic sex of mice across different mouse strains.
authors.affiliation: Division of Molecular Genetics and Development, Institute for Molecular Bioscience, The University of Queensland, Brisbane, Australia.
authors.forename: L
authors.initials: L
authors.lastname: McFarlane
authors.forename: V
authors.initials: V
authors.lastname: Truong
authors.forename: J S
authors.initials: JS
authors.lastname: Palmer
authors.forename: D
authors.initials: D
authors.lastname: Wilhelm
mesh_terms: D000818:Animals; D001483:Base Sequence; D005260:Female; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D011544:Pseudogenes / Q000235:genetics; D012732:Sex Determination Analysis / Q000379:methods*
chemical_list: D009687:Nuclear Proteins; C085792:Slx protein, mouse; C490092:Xlr nuclear protein, mouse