title: Solubilization of phenanthrene above cloud point of Brij 30: a new application in biodegradation.
abstract: In the present study a new application of solubilization of phenanthrene above cloud point of Brij 30 in biodegradation was developed. It was shown that a temporal solubilization of phenanthrene above cloud point of Brij 30 (5wt%) permitted to obtain a stable increase of the solubility of phenanthrene even when the temperature was decreased to culture conditions of used microorganism Pseudomonas putida (28°C). A higher initial concentration of soluble phenanthrene was obtained after the cloud point treatment: 200 against 120μM without treatment. All soluble phenanthrene was metabolized and a higher final concentration of its major metabolite - 1-hydroxy-2-naphthoic acid - (160 against 85μM) was measured in the culture medium in the case of a preliminary cloud point treatment. Therefore a temporary solubilization at cloud point might have a perspective application in the enhancement of biodegradation of polycyclic aromatic hydrocarbons.
authors.affiliation: Laboratoire Réactions et Génie des Procédés UPR CNRS 3349, University of Lorraine, ENSAIA - 2, Avenue de la Forêt de Haye, 54505 Vandoeuvre-les-Nancy Cedex, France. tatiana.v.p@hotmail.com
authors.forename: T
authors.initials: T
authors.lastname: Pantsyrnaya
authors.forename: S
authors.initials: S
authors.lastname: Delaunay
authors.forename: J L
authors.initials: JL
authors.lastname: Goergen
authors.forename: E
authors.initials: E
authors.lastname: Guseva
authors.forename: J
authors.initials: J
authors.lastname: Boudrant
mesh_terms: D001673:Biodegradation, Environmental; D009284:Naphthols; D044367:Phase Transition; D010616:Phenanthrenes / Q000737:chemistry*; D000077423:Polidocanol; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism; D011092:Polyethylene Glycols; D016958:Pseudomonas putida / Q000378:metabolism; D012995:Solubility; D013501:Surface-Active Agents / Q000737:chemistry / Q000378:metabolism
chemical_list: D009284:Naphthols; D010616:Phenanthrenes; D011084:Polycyclic Aromatic Hydrocarbons; D013501:Surface-Active Agents; D000077423:Polidocanol; D011092:Polyethylene Glycols; C031181:phenanthrene; C050175:1-hydroxy-2-naphthoic acid