title: Contribution of Aurora-A and -B expression to DNA aneuploidy in gastric cancers.
abstract: OBJECTIVE
  DNA aneuploidy, which is characterized by cells containing an abnormal number of chromosomes, is closely associated with carcinogenesis and malignant progression. Aneuploidy occurs during cell division when the chromosomes do not separate properly. Aurora kinases (Aurora-A, -B, and -C) contribute to accurate cell division, and are candidate molecular targets for mitosis-specific anticancer drugs.
  
  
  METHODS
  We determined the expression of Aurora-A and -B in 110 gastric cancer specimens by performing an immunohistochemical analysis. We also determined the DNA content, TP53 gene mutations, and microsatellite instability in the same samples.
  
  
  RESULTS
  We found the nuclear expression of Aurora-A and -B to increase in tumor tissue in comparison to that in normal epithelial tissue. A high Aurora-B expression significantly correlated with aneuploidy and TP53 mutations, but not with microsatellite instability. In contrast, the Aurora-A expression did not correlate with either aneuploidy or microsatellite instability. In addition, the expression of Aurora-A or -B was not significantly associated with the clinical outcomes or prognosis.
  
  
  CONCLUSIONS
  Our results suggest that an overexpression of Aurora-B, but not of Aurora-A, might contribute to DNA aneuploidy in gastric cancers by promoting chromosomal instability.
authors.affiliation: Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Honma
authors.forename: Ryota
authors.initials: R
authors.lastname: Nakanishi
authors.forename: Tomonori
authors.initials: T
authors.lastname: Nakanoko
authors.forename: Koji
authors.initials: K
authors.lastname: Ando
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Saeki
authors.forename: Eiji
authors.initials: E
authors.lastname: Oki
authors.forename: Makoto
authors.initials: M
authors.lastname: Iimori
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kitao
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Kakeji
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Maehara
mesh_terms: D000368:Aged; D000782:Aneuploidy; D064096:Aurora Kinase A / Q000235:genetics*; D064107:Aurora Kinase B / Q000235:genetics*; D004247:DNA / Q000235:genetics; D005260:Female; D015870:Gene Expression / Q000235:genetics*; D015971:Gene Expression Regulation, Enzymologic / Q000235:genetics*; D042822:Genomic Instability / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000201:enzymology* / Q000235:genetics*
chemical_list: D004247:DNA; D064096:Aurora Kinase A; D064107:Aurora Kinase B