title: Acute cerebral ischaemia: relationship between serum and cerebrospinal fluid orexin-A concentration and infarct volume.
abstract: OBJECTIVE
  Orexins are hypothalamic neuropeptides that are involved in feeding, neuroendocrine regulation, sleep-wakefulness and sleep disorders (such as narcolepsy). This study investigated the relationship between serum and cerebrospinal fluid (CSF) orexin-A concentrations and infarct volume, in patients with ischaemic stroke.
  
  
  METHODS
  Serum and CSF concentrations of orexin-A were determined 48-72 h after the onset of ischaemic stroke in patients, then compared with those of healthy control subjects of comparable age. Infarct volumes were measured using computerized tomography, 48-72 h after hospitalization.
  
  
  RESULTS
  Mean serum and CSF orexin-A concentrations were significantly lower among ischaemic stroke patients (n = 29) compared with control subjects (n = 13). There was a significant inverse correlation between infarct volumes and CSF orexin-A concentrations in patients with ischaemic stroke.
  
  
  CONCLUSIONS
  These data show that serum and CSF orexin-A concentrations decrease after cerebral ischaemia and may play a role in the development of brain injury. The orexin-A concentration in the CSF might be a useful biomarker for the assessment of progression of brain tissue damage during the early stages of ischaemic stroke.
authors.affiliation: Department of Neurology, Faculty of Medicine, Sakarya University, Sakarya, Turkey. dilcankotan@yahoo.com
authors.forename: Dilcan
authors.initials: D
authors.lastname: Kotan
authors.forename: Orhan
authors.initials: O
authors.lastname: Deniz
authors.forename: Recep
authors.initials: R
authors.lastname: Aygul
authors.forename: Abdulkadir
authors.initials: A
authors.lastname: Yildirim
mesh_terms: D000208:Acute Disease; D000368:Aged; D016022:Case-Control Studies; D002544:Cerebral Infarction / Q000097:blood* / Q000134:cerebrospinal fluid* / Q000473:pathology; D003710:Demography; D005260:Female; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000097:blood* / Q000134:cerebrospinal fluid*; D008297:Male; D008875:Middle Aged; D009479:Neuropeptides / Q000097:blood* / Q000134:cerebrospinal fluid*; D000068797:Orexins
chemical_list: C000596229:HCRT protein, human; D047908:Intracellular Signaling Peptides and Proteins; D009479:Neuropeptides; D000068797:Orexins
keywords: Cerebrospinal fluid; infarct volume; ischaemic stroke; neuropeptide hormone; orexin-A