title: Monitoring of KI and WU polyomaviruses in hematopoietic stem cell transplant patients.
abstract: Primary infection with KIPyV and WUPyV polyomaviruses occurs early in childhood followed by lifelong persistence in the body. Polyomavirus reactivation can occur in the presence of impaired immunity as in hematological malignancies or during immunosuppresssion induced by medications. In this study, reactivation of KIPyV and WUPyV was monitored by conventional PCR in plasma samples of 26 stem cell transplant patients and in 26 related bone marrow donors. Plasma samples from transplant patients were collected immediately after the end of conditioning regimen and up to 270 days after transplant. All plasma samples from transplant patients were negative for KIPyV and WUPyV DNA. Instead, KIPyV DNA was detected in two bone marrow donors. There was no evidence of KIPyV transmission from the donor to the recipient. The data suggest that detection of KIPyV in plasma is sporadic and that KPIyV and WUPyV do not affect the post-transplant clinical course. However, further studies on a larger sample size and more sensitive PCR methods are needed to confirm these observations.
authors.affiliation: Laboratory of Molecular Virology, Foundation Polyclinic Tor Vergata, Viale Oxford, Rome, Italy.
authors.forename: Rosa
authors.initials: R
authors.lastname: Porrovecchio
authors.forename: Muhammed
authors.initials: M
authors.lastname: Babakir-Mina
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Rapanotti
authors.forename: William
authors.initials: W
authors.lastname: Arcese
authors.forename: Carlo Federico
authors.initials: CF
authors.lastname: Perno
authors.forename: Marco
authors.initials: M
authors.lastname: Ciotti
mesh_terms: D000328:Adult; D004279:DNA, Viral / Q000097:blood*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D008875:Middle Aged; D019653:Myeloablative Agonists / Q000627:therapeutic use; D011120:Polyomavirus / Q000302:isolation & purification*; D027601:Polyomavirus Infections / Q000097:blood* / Q000276:immunology / Q000635:transmission; D014019:Tissue Donors; D019172:Transplantation Conditioning; D014412:Tumor Virus Infections / Q000097:blood* / Q000276:immunology / Q000635:transmission; D014775:Virus Activation
chemical_list: D004279:DNA, Viral; D019653:Myeloablative Agonists