title: MicroRNA-21 and -146b are involved in the pathogenesis of murine viral myocarditis by regulating TH-17 differentiation.
abstract: Viral myocarditis (VMC) is a common cardiovascular disease, and microRNAs (miRNAs) have been postulated to be involved in its pathology. Using microarrays, we observed that miRNA-21 and -146b were upregulated in a murine model of VMC. We also found that miRNA-451 was downregulated. In vivo silencing of miRNA-21 and -146b resulted in less-severe VMC. Overexpression of miRNA-451 did not ameliorate the severity of VMC. Further work revealed that inhibition of miRNA-21 and -146b decreased the expression levels of Th17 and RORγt . Overexpression of miRNA-451 had no effect on IL-17 and RORγt expression. Inhibition of miRNA-21 and -146b might ameliorate myocardium inflammation by mediating downregulation of RORγt expression, indicating that these miRNAs are involved in the pathogenesis of murine VMC.
authors.affiliation: Guangxi Cardiovascular Institute, Shuang-Yong Road 6, Nanning, 530021, People's Republic of China.
authors.forename: Yan Li
authors.initials: YL
authors.lastname: Liu
authors.forename: Weifeng
authors.initials: W
authors.lastname: Wu
authors.forename: Yimin
authors.initials: Y
authors.lastname: Xue
authors.forename: Mengsha
authors.initials: M
authors.lastname: Gao
authors.forename: Yuluan
authors.initials: Y
authors.lastname: Yan
authors.forename: Qing
authors.initials: Q
authors.lastname: Kong
authors.forename: Yu
authors.initials: Y
authors.lastname: Pang
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D003384:Coxsackievirus Infections / Q000235:genetics / Q000503:physiopathology / Q000821:virology; D004195:Disease Models, Animal; D029822:Enterovirus B, Human / Q000472:pathogenicity*; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D009205:Myocarditis / Q000235:genetics / Q000503:physiopathology / Q000821:virology; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3; D020411:Oligonucleotide Array Sequence Analysis; D058504:Th17 Cells / Q000166:cytology*; D015854:Up-Regulation
chemical_list: C542542:MIRN146 microRNA, human; C524940:MIRN21 microRNA, human; D035683:MicroRNAs; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3; C539901:Rorc protein, mouse