title: Triterpene glucosides from the leaves of Aralia elata and their cytotoxic activities.
abstract: Three new triterpene glucosides, named congmuyenosides C-E (1-3, resp.), along with four known ones, were isolated from an EtOH extract of Aralia elata (Miq.) Seem. leaves. The structures of the new compounds were identified as 3-O-{β-D-glucopyranosyl-(1→3)-β-D-glucopyranosyl-(1→3)-[β-D-glucopyranosyl-(1→2)]-β-D-glucopyranosyl}caulophyllogenin (1), 3-O-{β-D-glucopyranosyl-(1→3)-β-D-glucopyranosyl-(1→3)-[β-D-glucopyranosyl-(1→2)]-β-D-glucopyranosyl}hederagenin 28-O-β-D-glucopyranosyl ester (2), 3-O-{β-D-glucopyranosyl-(1→3)-β-D-glucopyranosyl-(1→3)-[β-D-glucopyranosyl-(1→2)]-β-D-glucopyranosyl}echinocystic acid 28-O-β-D-glucopyranosyl ester (3) on the basis of spectral analyses, including MS, (1) H-NMR, (13) C-NMR, DEPT, HSQC, HMBC, NOESY, and HSQC-TOCSY experiments. All isolates obtained were evaluated for their cytotoxic activities against three human tumor cell lines (HepG2, SKOV3, and A549). Compound 3 showed significant cytotoxicity against A549 cell line (IC50 9.9±1.5 μM).
authors.affiliation: Key Laboratory of Chinese Materia Medica, Ministry of Education, Heilongjiang University of Chinese Medicine, No. 24 Heping Road, Xiangfang District, Harbin 150040, P. R. China. hxkuang@hotmail.com
authors.forename: Hai-Xue
authors.initials: HX
authors.lastname: Kuang
authors.forename: Zhi-Bin
authors.initials: ZB
authors.lastname: Wang
authors.forename: Qiu-Hong
authors.initials: QH
authors.lastname: Wang
authors.forename: Bing-You
authors.initials: BY
authors.lastname: Yang
authors.forename: Hong-Bin
authors.initials: HB
authors.lastname: Xiao
authors.forename: Yoshihito
authors.initials: Y
authors.lastname: Okada
authors.forename: Tohru
authors.initials: T
authors.lastname: Okuyama
mesh_terms: D027983:Aralia / Q000737:chemistry*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005960:Glucosides / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D056945:Hep G2 Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D018515:Plant Leaves / Q000737:chemistry; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity
chemical_list: D005960:Glucosides; D014315:Triterpenes; C583761:congmuyenoside C; C583763:congmuyenoside D; C583764:congmuyenoside E