title: Murine macrophage inflammatory cytokine production and immune activation in response to Vibrio parahaemolyticus infection.
abstract: Vibrio parahaemolyticus is the most common cause of bacterial, seafood-related illness in the USA. Currently, there is a dearth of published reports regarding immunity to infection with this pathogen. Here, production of both pro- and anti-inflammatory cytokines by V. parahaemolyticus-infected RAW 264.7 murine macrophages was studied. It was determined that this infection results in increased concentrations of IL-1α, IL-6, TNF-α and IL-10. Additionally, decreases in cell surface TLR2 and TLR4 and increases in T-cell co-stimulatory molecules CD40 and CD86 were discovered. The data presented here begin to identify the immune variables required to eliminate V. parahaemolyticus from infected host tissues.
authors.affiliation: Department of Biological Sciences, Wolf Hall, College of Arts and Sciences, University of Delaware, Newark, Delaware 19716, USA.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Waters
authors.forename: Sanjana
authors.initials: S
authors.lastname: Luther
authors.forename: Torsten
authors.initials: T
authors.lastname: Joerger
authors.forename: Gary P
authors.initials: GP
authors.lastname: Richards
authors.forename: E Fidelma
authors.initials: EF
authors.lastname: Boyd
authors.forename: Michelle A
authors.initials: MA
authors.lastname: Parent
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D005786:Gene Expression Regulation; D016130:Immunophenotyping; D018836:Inflammation Mediators / Q000378:metabolism*; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism* / Q000821:virology; D051379:Mice; D014735:Vibrio Infections / Q000235:genetics / Q000276:immunology*; D014736:Vibrio parahaemolyticus / Q000276:immunology*
chemical_list: D000954:Antigens, Surface; D016207:Cytokines; D018836:Inflammation Mediators