title: A new multichannel spectral imaging laser scanning confocal microscope.
abstract: We have developed a new multichannel spectral imaging laser scanning confocal microscope for effective detection of multiple fluorescent labeling in the research of biological tissues. In this paper, the design and key technologies of the system are introduced. Representative results on confocal imaging, 3-dimensional sectioning imaging, and spectral imaging are demonstrated. The results indicated that the system is applicable to multiple fluorescent labeling in biological experiments.
authors.affiliation: Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China.
authors.forename: Yunhai
authors.initials: Y
authors.lastname: Zhang
authors.forename: Bian
authors.initials: B
authors.lastname: Hu
authors.forename: Yakang
authors.initials: Y
authors.lastname: Dai
authors.forename: Haomin
authors.initials: H
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Xue
authors.forename: Fazhi
authors.initials: F
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Chenyu
authors.initials: C
authors.lastname: Jiang
authors.forename: Fei
authors.initials: F
authors.lastname: Gao
authors.forename: Jian
authors.initials: J
authors.lastname: Chang
mesh_terms: D000818:Animals; D004247:DNA / Q000378:metabolism; D003952:Diagnostic Imaging / Q000379:methods; D004867:Equipment Design; D005456:Fluorescent Dyes / Q000494:pharmacology; D007091:Image Processing, Computer-Assisted / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000379:methods; D007211:Indoles / Q000494:pharmacology; D007668:Kidney / Q000473:pathology; D007834:Lasers; D051379:Mice; D018613:Microscopy, Confocal / Q000295:instrumentation* / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods; D055095:Optics and Photonics / Q000379:methods; D051381:Rats
chemical_list: D005456:Fluorescent Dyes; D007211:Indoles; C007293:DAPI; D004247:DNA