title: Rapid purification of Soybean mosaic virus from small quantities of tissue by immunoprecipitation.
abstract: A rapid, simple, and efficient method for purification of Soybean mosaic virus (SMV) was developed based on immunoprecipitation. Traditional centrifugation-based methods for purification of SMV and other potyviruses require long, complicated procedures and large quantities of infected tissue (100-500 g). The immunoprecipitation procedure described in this study allows the purification of intact SMV virion particles in 4h from 0.5 g of tissue. The reliability of this procedure was demonstrated by RT-PCR and transmission electron microscopy (TEM). This method will be useful for high-throughput examination of the physical and morphological properties of virus particles because intact virion preparations ready for TEM observation can be purified rapidly from very small tissue samples.
authors.affiliation: Department of Agricultural Biotechnology and Plant Genomics and Breeding Institute, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Jang-Kyun
authors.initials: JK
authors.lastname: Seo
authors.forename: Minji
authors.initials: M
authors.lastname: Kang
authors.forename: Mi Sa
authors.initials: MS
authors.lastname: Vo Phan
authors.forename: Kook-Hyung
authors.initials: KH
authors.lastname: Kim
mesh_terms: D047468:Immunoprecipitation / Q000379:methods*; D046529:Microscopy, Electron, Transmission; D017800:Potyvirus / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013025:Glycine max / Q000821:virology*; D014773:Virology / Q000379:methods*