title: Sodium taurocholate cotransporting polypeptide mediates woolly monkey hepatitis B virus infection of Tupaia hepatocytes.
abstract: Primary Tupaia hepatocytes (PTHs) are susceptible to woolly monkey hepatitis B virus (WMHBV) infection, but the identity of the cellular receptor(s) mediating WMHBV infection of PTHs remains unclear. Recently, sodium taurocholate cotransporting polypeptide (NTCP) was identified as a functional receptor for human hepatitis B virus (HBV) infection of primary human and Tupaia hepatocytes. In this study, a synthetic pre-S1 peptide from WMHBV was found to bind specifically to cells expressing Tupaia NTCP (tsNTCP) and it efficiently blocked WMHBV entry into PTHs; silencing of tsNTCP in PTHs significantly inhibited WMHBV infection. Ectopic expression of tsNTCP rendered HepG2 cells susceptible to WMHBV infection. These data demonstrate that tsNTCP is a functional receptor for WMHBV infection of PTHs. The result also indicates that NTCP's orthologs likely act as a common cellular receptor for all known primate hepadnaviruses.
authors.affiliation: National Institute of Biological Sciences, Beijing, China.
authors.forename: Guocai
authors.initials: G
authors.lastname: Zhong
authors.forename: Huan
authors.initials: H
authors.lastname: Yan
authors.forename: Haimin
authors.initials: H
authors.lastname: Wang
authors.forename: Wenhui
authors.initials: W
authors.lastname: He
authors.forename: Zhiyi
authors.initials: Z
authors.lastname: Jing
authors.forename: Yonghe
authors.initials: Y
authors.lastname: Qi
authors.forename: Liran
authors.initials: L
authors.lastname: Fu
authors.forename: Zhenchao
authors.initials: Z
authors.lastname: Gao
authors.forename: Yi
authors.initials: Y
authors.lastname: Huang
authors.forename: Guangwei
authors.initials: G
authors.lastname: Xu
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Feng
authors.forename: Jianhua
authors.initials: J
authors.lastname: Sui
authors.forename: Wenhui
authors.initials: W
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D053819:Atelinae / Q000821:virology*; D002478:Cells, Cultured; D015613:Hepadnaviridae / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D018347:Hepadnaviridae Infections / Q000821:virology; D006514:Hepatitis B Surface Antigens / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D022781:Hepatocytes / Q000821:virology*; D006801:Humans; D008969:Molecular Sequence Data; D029363:Organic Anion Transporters, Sodium-Dependent / Q000378:metabolism*; D011498:Protein Precursors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011991:Receptors, Virus / Q000378:metabolism*; D027981:Symporters / Q000378:metabolism*; D014417:Tupaia / Q000821:virology*
chemical_list: D006514:Hepatitis B Surface Antigens; D029363:Organic Anion Transporters, Sodium-Dependent; D011498:Protein Precursors; D011991:Receptors, Virus; D027981:Symporters; C050806:presurface protein 1, hepatitis B surface antigen; C071908:sodium-bile acid cotransporter