title: Dioscorin pre-treatment protects A549 human airway epithelial cells from hydrogen peroxide-induced oxidative stress.
abstract: Hydrogen peroxide (H(2)O(2)) is a highly reactive oxygen species involved in lung and bronchial epithelium injury. Increased H(2)O(2) levels have been reported in expired breath condensates of patients with inflammatory airway diseases such as chronic obstructive pulmonary disease. Protecting airway epithelial cells from oxidative stress is an important task in the prevention and management of airway diseases. Previous studies demonstrate that yam (Dioscorea batatas Decne) has antioxidant and anti-trypsin activities. This study evaluated the validity of dioscorin in vitro. The results showed that dioscorin attenuated the alteration of H(2)O(2) on G2/M cell cycle arrest. This might be associated with the activation of IκB and subsequent inactivation of NF-κB. Furthermore, dioscorin suppressed IL-8 secretion and reduced changes of adhesion molecule expressions in H(2)O(2)-injured A549 cells. These results help in understanding the potential of traditional Chinese herbal medicine as treatment for airway inflammatory diseases.
authors.affiliation: Division of Chest Medicine, Taichung Veterans General Hospital, Taichung, Taiwan.
authors.forename: Jeng-Yuan
authors.initials: JY
authors.lastname: Hsu
authors.forename: Jao-Jia
authors.initials: JJ
authors.lastname: Chu
authors.forename: Ming-Chih
authors.initials: MC
authors.lastname: Chou
authors.forename: Ya-Wen
authors.initials: YW
authors.lastname: Chen
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D015815:Cell Adhesion Molecules / Q000096:biosynthesis; D002460:Cell Line; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D006861:Hydrogen Peroxide; D051550:I-kappa B Kinase / Q000378:metabolism; D039421:Integrin alpha2 / Q000378:metabolism; D039503:Integrin alpha6 / Q000378:metabolism; D019012:Integrin beta1 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D059566:M Phase Cell Cycle Checkpoints / Q000187:drug effects; D008516:Medicine, Chinese Traditional; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010940:Plant Proteins / Q000494:pharmacology*; D029424:Pulmonary Disease, Chronic Obstructive / Q000188:drug therapy; D017382:Reactive Oxygen Species; D020545:Respiratory Mucosa / Q000166:cytology / Q000187:drug effects* / Q000473:pathology
chemical_list: D000975:Antioxidants; C508606:CXCL8 protein, human; D015815:Cell Adhesion Molecules; D018960:Hyaluronan Receptors; D039421:Integrin alpha2; D039503:Integrin alpha6; D019012:Integrin beta1; D016209:Interleukin-8; D016328:NF-kappa B; D010940:Plant Proteins; D017382:Reactive Oxygen Species; C095057:dioscorin protein, Dioscorea; D006861:Hydrogen Peroxide; D051550:I-kappa B Kinase