title: Fibroblasts from women with pelvic organ prolapse show differential mechanoresponses depending on surface substrates.
abstract: OBJECTIVE
  Little is known about dynamic cell-matrix interactions in the context of pathophysiology and treatments for pelvic organ prolapse (POP). This study sought to identify differences between fibroblasts from women with varying degrees of prolapse in reaction to mechanical stimuli and matrix substrates in vitro.
  
  
  METHODS
  Fibroblasts from the vaginal wall of three patients with POP Quantification (POP-Q) system stages 0, II, and IV were stretched on artificial polymer substrates either coated or not coated with collagen I. Changes in morphology and anabolic/catabolic compounds that affect matrix remodelling were evaluated at protein- and gene-expression levels. Statistical analysis was performed using one-way analysis of variance (ANOVA), followed by Tukey-Kramer's post hoc test.
  
  
  RESULTS
  POP fibroblasts show delayed cell alignment and lower responses to extracellular matrix remodelling factors at both enzymatic- and gene-expression levels compared with healthy fibroblasts.
  
  
  CONCLUSIONS
  POP fibroblasts, when compared with healthy cells, show differential mechanoresponses on two artificial polymer substrates. This should be taken into account when designing or improving implants for treating POP.
authors.affiliation: Department of Orthopaedics, VU University Medical Center, Research Institute MOVE, Amsterdam, The Netherlands. a.ruiz@acta.nl
authors.forename: Alejandra M
authors.initials: AM
authors.lastname: Ruiz-Zapata
authors.forename: Manon H
authors.initials: MH
authors.lastname: Kerkhof
authors.forename: Behrouz
authors.initials: B
authors.lastname: Zandieh-Doulabi
authors.forename: Hans A M
authors.initials: HA
authors.lastname: Brölmann
authors.forename: Theo H
authors.initials: TH
authors.lastname: Smit
authors.forename: Marco N
authors.initials: MN
authors.lastname: Helder
mesh_terms: D001696:Biomechanical Phenomena / Q000502:physiology*; D001706:Biopsy; D021982:Cell-Matrix Junctions / Q000473:pathology* / Q000502:physiology; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism; D005109:Extracellular Matrix / Q000473:pathology / Q000502:physiology; D005260:Female; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D066298:In Vitro Techniques; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D056887:Pelvic Organ Prolapse / Q000473:pathology* / Q000503:physiopathology; D011108:Polymers; D012720:Severity of Illness Index; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000378:metabolism; D014621:Vagina / Q000473:pathology
chemical_list: D024042:Collagen Type I; D011108:Polymers; D019716:Tissue Inhibitor of Metalloproteinase-2; D020778:Matrix Metalloproteinase 2