title: Effect of siRNA targeting MTA1 on metastasis malignant phenotype of ovarian cancer A2780 cells.
abstract: Ovarian cancer is the fifth lethal gynecologic malignancy. Metastasis-associated gene 1 (MTA1) is overexpressed in many malignant tumors with high metastatic potential. This study investigated whether down-regulation of MTA1 expression by RNAi in A2780 ovarian cancer cells could affect proliferation, anoikis, migration, invasion and adhesion of the cells and to research the potential for MTA1 gene therapy of ovarian cancer. After transfection with effective Mta1 gene siRNA, the effects on proliferation, anoikis, migration, invasion and adhesion of A2780 cells were tested by MTT assay, flow cytometry, wound-healing assay, Transwell assay and adhesion assay. Expression levels of PTEN, beta 1 integrin, MMP-9, phosphor-AKT (Ser473), and total AKT activity were evaluated in control and transfected cells. The results showed that inhibition of MTA1 mediated by Mta1-siRNA transfection decreased the cell invasion, migration and adhesion, and induced the increased cell anoikis, but no significant difference was found in proliferation of A2780 cancer cells. In addition, beta 1 integrin, MMP-9, and phosphor-AKT protein levels were significantly down-regulated, while PTEN was significantly up-regulated. These results demonstrated that MTA1 played an important role in the cell metastasis in ovarian cancer. MTA1 could serve as another novel potential therapeutic target in ovarian cancer.
authors.affiliation: Department of Obstetrics and Gynecology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
authors.forename: Yu-Mei
authors.initials: YM
authors.lastname: Rao
authors.affiliation: Department of Obstetrics and Gynecology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
authors.forename: Mei
authors.initials: M
authors.lastname: Ji
authors.affiliation: Center of Reproductive Medicine, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
authors.forename: Cai-Hong
authors.initials: CH
authors.lastname: Chen
authors.affiliation: Department of Obstetrics and Gynecology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China. huirongshi@yahoo.com.cn.
authors.forename: Hui-Rong
authors.initials: HR
authors.lastname: Shi
mesh_terms: D017209:Apoptosis / Q000235:genetics; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology / Q000556:secondary*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000235:genetics; D005260:Female; D018390:Gene Targeting / Q000379:methods; D015316:Genetic Therapy / Q000379:methods; D006655:Histone Deacetylases / Q000235:genetics*; D006801:Humans; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000473:pathology* / Q000628:therapy; D034741:RNA, Small Interfering / Q000235:genetics* / Q000627:therapeutic use; D012097:Repressor Proteins / Q000235:genetics*; D015534:Trans-Activators; D016896:Treatment Outcome
chemical_list: C089320:MTA1 protein, human; D034741:RNA, Small Interfering; D012097:Repressor Proteins; D015534:Trans-Activators; D006655:Histone Deacetylases