title: The targeting, docking and anti-proteolysis functions of the secretin chaperone PulS.
abstract: The Klebsiella oxytoca lipoprotein PulS might function as either or both a pilot and a docking factor in the outer membrane targeting and assembly of the Type II secretion system secretin PulD. In the piloting model, PulS binds to PulD monomers and targets them to the outer membrane via the lipoprotein sorting pathway components LolA and LolB. In this model, PulS also protects the PulD monomers from proteolysis during transit through the periplasm. In the docking model, PulS is targeted alone to the outer membrane, where it acts as a receptor for PulD monomers, allowing them to accumulate and assemble specifically in this membrane. PulS was shown to dissociate from and/or re-associate freely with PulD multimers in zwitterionic detergent, making it difficult to determine whether PulS remains associated with PulD dodecamers in the outer membrane by co-purification. However, PulD protomers in the dodecamer were shown to be stable in the absence of PulS, indicating that PulS is only required to protect the protease-susceptible monomer. DegP was identified as one of the proteases that could contribute to PulD degradation in the absence of PulS. Studies on the in vitro assembly and targeting of PulD into Escherichia coli membrane vesicles demonstrated its strong preference to insert into the inner membrane, as is the case in vivo in the absence of PulS. However, PulD could be targeted to outer membrane fragments in vitro if they were preloaded with PulS, indicating the technical feasibility of the docking model. We conclude that both modes of action might contribute to efficient outer membrane targeting of PulD in vivo, although the piloting function is likely to predominate.
authors.affiliation: Institut Pasteur, Molecular Genetics Unit, 25 rue du Dr. Roux, 70105 Paris, France.
authors.forename: Séverine
authors.initials: S
authors.lastname: Collin
authors.forename: Martin
authors.initials: M
authors.lastname: Krehenbrink
authors.forename: Ingrid
authors.initials: I
authors.lastname: Guilvout
authors.forename: Anthony P
authors.initials: AP
authors.lastname: Pugsley
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D058947:Bacterial Secretion Systems; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism*; D041121:Klebsiella oxytoca / Q000378:metabolism*; D008954:Models, Biological; D018832:Molecular Chaperones / Q000378:metabolism*; D011480:Protease Inhibitors / Q000378:metabolism*; D055503:Protein Multimerization; D021381:Protein Transport; D059748:Proteolysis
chemical_list: D001425:Bacterial Outer Membrane Proteins; D058947:Bacterial Secretion Systems; D029968:Escherichia coli Proteins; D018832:Molecular Chaperones; D011480:Protease Inhibitors; C560058:PulD protein, E coli; C560059:PulS protein, E coli; C422473:PulS protein, Klebsiella oxytoca