title: Cullin1 is a novel marker of poor prognosis and a potential therapeutic target in human breast cancer.
abstract: BACKGROUND
  To investigate the role of Cullin1 (Cul1) in the development of breast cancer, we examined the expression of Cul1 in breast cancer tissues and analyzed the correlation between Cul1 expression and clinicopathologic variables and patients survival.
  
  
  METHODS
  We evaluated the Cul1 expression by immunohistochemistry using a tissue microarray (TMA) which includes 393 breast cancer tissues. We also studied the role of Cul1 in breast cancer cell proliferation, migration and invasion by carrying out CCK8 cell proliferation assay, cell migration and invasion assay.
  
  
  RESULTS
  The Cul1 expression was significantly correlated with breast cancer histology grade (P = 0.000), estrogen receptor status (P = 0.001), progesterone receptor status (P = 0.001) and human epidermal growth factor receptor 2 status (P = 0.002). Furthermore, we showed a strong correlation between high Cul1 expression and worse 5-year overall and disease-specific survival rates in breast cancer patients (P = 0.026 and P = 0.015, respectively). Finally, we found that Cul1 knockdown inhibits cell proliferation, migration and invasion abilities.
  
  
  CONCLUSIONS
  Cul1 overexpression is significantly correlated with breast cancer progression and predicts worse survival. Cul1 regulates breast cancer cell proliferation, migration and invasion.
authors.affiliation: Jiangsu Key Laboratory of Biological Cancer Therapy, The Affiliated Hospital of Xuzhou Medical College, Xuzhou Medical College, Xuzhou, Jiangsu, China.
authors.forename: J
authors.initials: J
authors.lastname: Bai
authors.forename: H M
authors.initials: HM
authors.lastname: Yong
authors.forename: F F
authors.initials: FF
authors.lastname: Chen
authors.forename: P J
authors.initials: PJ
authors.lastname: Mei
authors.forename: H
authors.initials: H
authors.lastname: Liu
authors.forename: C
authors.initials: C
authors.lastname: Li
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Pan
authors.forename: Y P
authors.initials: YP
authors.lastname: Wu
authors.forename: J N
authors.initials: JN
authors.lastname: Zheng
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000401:mortality* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D044842:Cullin Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics; D011379:Prognosis; D034622:RNA Interference; D034741:RNA, Small Interfering; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism; D011960:Receptors, Estrogen / Q000378:metabolism; D011980:Receptors, Progesterone / Q000378:metabolism; D013534:Survival; D046888:Tissue Array Analysis; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000378:metabolism
chemical_list: D014408:Biomarkers, Tumor; D044842:Cullin Proteins; D020778:Matrix Metalloproteinase 2; D034741:RNA, Small Interfering; D018719:Erb-b2 Receptor Tyrosine Kinases; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D019716:Tissue Inhibitor of Metalloproteinase-2; C100318:Cullin 1; C560823:TIMP2 protein, human; C508053:ERBB2 protein, human
keywords: breast cancer; cullin1; migration, invasion; prognostic; proliferation