title: [Study on immune status of patients with schistosomiasis japonica in Poyang Lake region II characterization of cellular immune in schistosomiasis patients].
abstract: OBJECTIVE
  To observe the characterization of cellular immune of schistosomiasis japonica patients in low endemic area of Poyang Lake region.
  
  
  METHODS
  The schistosomiasis patients who were positive by stool detections were selected as study subjects, and the individuals who were negative by stool detections in three consecutive years were selected as negative controls. The CD4+T cell, CD8+T cell and CD4+CD25+Treg in venous blood of the study subjects were detected by using flow cytometry. In vitro, cytokine (IFN-gamma and IL-10) production of PBMC in response to soluble egg antigen (SEA) and soluble worm antigen preparation (SWA) were measured by ELISA.
  
  
  RESULTS
  The levels of CD4+CD25+Treg in individuals who were positive by stool detections (5.99 +/- 1.60)% were higher than those of the negative (5.04 +/- 1.64)%, and the difference was significant (t = 3.261, P < 0.01). One year after the treatment, the levels of IFN-gamma were significantly higher (Stimulated by SEA before and after the treatment, the mean levels were 121.97 and 2 564.03 pg/ml, respectively; stimulated by SWA before and after treatment, the mean levels were 102.89 and 646.26 pg/ml, respectively) and IL-10 were significant lower in patients who were positive by stool detections (Stimulated by SEA before and after treatment, the mean level were 294.75 and 122.43 pg/ml, respectively; stimulated by SWAP before and after treatment, the mean cytokine levels were 110.10 and 31.85 pg/ml, respectively).
  
  
  CONCLUSIONS
  The increase of induced CD4+CD25+Treg is one of causes that down-regulate cellular immune of human body after infection with Schistosoma japonicum.
authors.affiliation: Jiangxi Provincial Institute of Parasitic Diseases Control and Prevention, Nanchang 330046, China.
authors.forename: Min
authors.initials: M
authors.lastname: Yuan
authors.forename: Shu-Ying
authors.initials: SY
authors.lastname: Xie
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Zhou
authors.forename: Fei
authors.initials: F
authors.lastname: Hu
authors.forename: Yue-Min
authors.initials: YM
authors.lastname: Liu
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Zeng
authors.forename: Zhao-Jun
authors.initials: ZJ
authors.lastname: Li
authors.forename: Yi-Feng
authors.initials: YF
authors.lastname: Li
authors.forename: Zu-Lu
authors.initials: ZL
authors.lastname: Gao
authors.forename: Jian-Ying
authors.initials: JY
authors.lastname: Li
authors.forename: Hong-Gen
authors.initials: HG
authors.lastname: Chen
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Lin
mesh_terms: D000328:Adult; D000947:Antigens, Helminth / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002681:China; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007111:Immunity, Cellular / Q000187:drug effects / Q000276:immunology*; D060106:Lakes / Q000469:parasitology*; D008297:Male; D012554:Schistosomiasis japonica / Q000188:drug therapy / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology
chemical_list: D000947:Antigens, Helminth