title: Effects on high cholesterol-fed to liver, retina, hippocampus, and Harderian gland in Goto-Kakizaki rat.
abstract: To understand the relationship among cholesterolemia, hyperglycemic stage in non obese type 2 diabetes mellitus, and histological perturbations on liver, retina, hippocampus, and Harderian gland, we maintained rat on a diet high in cholesterol for fourteen weeks, then analyzed blood lipid profiles, blood glucose, hepatic enzymes, and microscopic lesion of those tissues. We observed that high cholesterol-treated rat elevated in cholesterol and low density lipoprotein with not correlated to hyperglycemia. Histopathological changing in Goto-Kakizaki rat on liver (microvesicular steatosis) and Harderain gland (tubular lesions) were related to hyperglycemic effect rather than cholesterolemic effect. These may be related to hypoinsulinemic characteristic of this diabetic model. However increasing pyknotic nuclei on hippocampus and reducing of retinal ganglionic cell were related to the high level of cholesterol loaded with synergized effect due to diabetic stage.
authors.affiliation: Academic Services Office, National Laboratory Animal Center, Mahidol University 999 Salaya, Puttamonthon, Nakorn Pathom, 73170, Thailand.
authors.forename: Kanchana
authors.initials: K
authors.lastname: Kengkoom
authors.forename: Aekkarin
authors.initials: A
authors.lastname: Klinkhamhom
authors.forename: Aunchalee
authors.initials: A
authors.lastname: Sirimontaporn
authors.forename: Ornuma
authors.initials: O
authors.lastname: Singha
authors.forename: Taweesak
authors.initials: T
authors.lastname: Ketjareon
authors.forename: Yaowaluk
authors.initials: Y
authors.lastname: Panavechkijkul
authors.forename: Dutmanee
authors.initials: D
authors.lastname: Seriwatanachai
authors.forename: Suluck
authors.initials: S
authors.lastname: Ukong
authors.forename: Sumate
authors.initials: S
authors.lastname: Ampawong
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D002467:Cell Nucleus / Q000187:drug effects / Q000473:pathology; D002791:Cholesterol, Dietary / Q000494:pharmacology*; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism* / Q000473:pathology*; D004195:Disease Models, Animal; D005260:Female; D005724:Ganglia / Q000187:drug effects / Q000473:pathology; D006243:Harderian Gland / Q000187:drug effects* / Q000473:pathology; D006624:Hippocampus / Q000187:drug effects* / Q000473:pathology; D007328:Insulin / Q000097:blood; D008055:Lipids / Q000097:blood; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D051381:Rats; D011919:Rats, Inbred Strains; D017208:Rats, Wistar; D012160:Retina / Q000187:drug effects* / Q000473:pathology
chemical_list: D001786:Blood Glucose; D002791:Cholesterol, Dietary; D007328:Insulin; D008055:Lipids; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase
keywords: Goto-Kakizaki rat; Harderian gland; High cholesterol; hippocampus; liver; retina