title: Extremely selective "turn-on" fluorescence detection of hypochlorite confirmed by proof-of-principle neurological studies via esterase action in living cells.
abstract: Highly specific and sensitive fluorescence detection of hypochlorite in nonbiotic pure water (rapid "turn-on", ~400 fold, λ(em) ~ 560 nm) as well as in living neuronal cell cultures (neutral pH) involves oxidation of a 2-sulfide-2-benzoic acid pendent group in a new meso-thienyl-BODIPY donor-acceptor probe.
authors.affiliation: Department of Chemistry, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Atul P
authors.initials: AP
authors.lastname: Singh
authors.forename: Olga G
authors.initials: OG
authors.lastname: Tsay
authors.forename: Dhiraj P
authors.initials: DP
authors.lastname: Murale
authors.forename: Taehong
authors.initials: T
authors.lastname: Jun
authors.forename: Hyunjeong
authors.initials: H
authors.lastname: Liew
authors.forename: Yoo-Hun
authors.initials: YH
authors.lastname: Suh
authors.forename: David G
authors.initials: DG
authors.lastname: Churchill
mesh_terms: D001557:Benzenesulfonates / Q000737:chemistry; D001565:Benzoates / Q000737:chemistry; D001896:Boron Compounds / Q000737:chemistry*; D002470:Cell Survival; D004950:Esterases / Q000378:metabolism*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D006997:Hypochlorous Acid / Q000032:analysis*; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D009447:Neuroblastoma / Q000201:enzymology* / Q000378:metabolism / Q000473:pathology; D010084:Oxidation-Reduction; D017382:Reactive Oxygen Species / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001557:Benzenesulfonates; D001565:Benzoates; D001896:Boron Compounds; D005456:Fluorescent Dyes; D017382:Reactive Oxygen Species; D006997:Hypochlorous Acid; D004950:Esterases; C028805:2-sulfobenzoic acid