title: CdTe-TiO2 nanocomposite: an impeder of bacterial growth and biofilm.
abstract: The resurgence of infectious diseases and associated issues related to antibiotic resistance has raised enormous challenges which may possibly be confronted primarily by nanotechnology routes. One key need of critical significance in this context is the development of an agent capable of inhibiting quorum sensing mediated biofilm formation in pathogenic organisms. In this work we examine the possible use of a nanocomposite, CdTe-TiO2, as an impeder of growth and biofilm. In the presence of CdTe-TiO2, scanning electron microscopy (SEM) analysis shows exposed cells without the surrounding matrix. Confocal laser scanning microscopy shows spatially distributed fluorescence, a typical indication of an impeded biofilm, as opposed to the control which shows matrix-covered cells and continuous fluorescence, typical of biofilm formation. Quantitatively, the inhibition of biofilm was ∼57%. CdTe-TiO2 also exhibits good antibacterial properties against Gram positive and Gram negative organisms by virtue of the generation of reactive oxygen species inside the cells, reflected by a ruptured appearance in the SEM analysis.
authors.affiliation: National Chemical Laboratory (CSIR-NCL), Dr Homi Bhabha Road, Pashan, Pune, India.
authors.forename: Haribhau
authors.initials: H
authors.lastname: Gholap
authors.forename: Rajendra
authors.initials: R
authors.lastname: Patil
authors.forename: Prasad
authors.initials: P
authors.lastname: Yadav
authors.forename: Arun
authors.initials: A
authors.lastname: Banpurkar
authors.forename: Satishchandra
authors.initials: S
authors.lastname: Ogale
authors.forename: Wasudeo
authors.initials: W
authors.lastname: Gade
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D001424:Bacterial Infections / Q000188:drug therapy; D018441:Biofilms / Q000187:drug effects*; D019187:Cadmium Compounds / Q000737:chemistry / Q000494:pharmacology*; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D053761:Nanocomposites / Q000737:chemistry / Q000648:ultrastructure; D011550:Pseudomonas aeruginosa / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D045663:Quantum Dots; D053038:Quorum Sensing / Q000187:drug effects; D013691:Tellurium / Q000737:chemistry / Q000494:pharmacology*; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D019187:Cadmium Compounds; C009495:titanium dioxide; D014025:Titanium; D013691:Tellurium; C028337:cadmium telluride