title: [Effects of blueberry on the expression of PPARγ and PDGF-B in rat hepatic fibrosis].
abstract: OBJECTIVE
  To explore the effects of blueberry on the expressions of peroxisome proliferator-activated receptor γ (PPARγ) and platelet-derived growth factor B (PDGF-B) in rat hepatic fibrosis.
  
  
  METHODS
  A total of 45 male Sprague-Dawley rats were randomly divided into control group, CCl4-induced hepatic fibrosis (model group), blueberry prevention (BB group), DSHX prevention (DSHX group) and blueberry+DSHX prevention (BB+DSHX group) (n = 9 each). Fibrous liver models of rats were induced by subcutaneous injection of CCl4 and high-lipid/low-protein diet for 8 weeks except for control group. Then the expressions of collagen I (ColI), PPARγ and PDGF-B were determined by real-time polymerase chain reaction (RT-PCR), immunohistochemistry and Western blot respectively.
  
  
  RESULTS
  Compared with the control group, the expression of PPARγ decreased and those of ColI and PDGF-B were elevated in model group (P < 0.05). The expression of PPARγ increased and ColI and PDGF-B decreased in BB, DSHX and BB+DSHX groups as compared to model group (P < 0.05).
  
  
  CONCLUSIONS
  The inhibitory effects of blueberry in CCl4-induced liver fibrosis may be correlated with the activation of PPARγ, the inhibited expression of PDGF-B and the reduced synthesis of extracellular matrix.
authors.affiliation: Department of Clinical Microbiology & Immunology, Guiyang Medical College, Guiyang, China.
authors.forename: Yu-ping
authors.initials: YP
authors.lastname: Wang
authors.forename: Ming-liang
authors.initials: ML
authors.lastname: Cheng
authors.forename: Bao-fang
authors.initials: BF
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
mesh_terms: D000818:Animals; D029798:Blueberry Plants; D024042:Collagen Type I / Q000378:metabolism; D008106:Liver Cirrhosis, Experimental / Q000378:metabolism*; D008297:Male; D047495:PPAR gamma / Q000378:metabolism*; D020574:Proto-Oncogene Proteins c-sis / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D024042:Collagen Type I; D047495:PPAR gamma; D020574:Proto-Oncogene Proteins c-sis