title: Analysis of protein interactions using fluorescence technologies.
abstract: Biophotonics techniques, especially those involving fluorescence, are widely used in proteomics to characterize the in vitro interactions between proteins in high-throughput mode. On the other hand, fluorescence-based imaging studies often show that protein activity is regulated through large protein complexes that transiently form at specific sites in the cell. One could therefore argue that a systematic functional analysis of the human proteome requires technologies that are capable of time and spatially resolved, multiplexed analysis of protein interactions within cells.
authors.affiliation: Department of Physiology, University of Wisconsin-Madison, 1300 University Ave, Madison, WI 53706, USA.
authors.forename: Yuling
authors.initials: Y
authors.lastname: Yan
authors.forename: Gerard
authors.initials: G
authors.lastname: Marriott
mesh_terms: D000818:Animals; D005453:Fluorescence; D005454:Fluorescence Polarization; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D025941:Protein Interaction Mapping / Q000379:methods*