title: Virus-like particle vaccine induces cross-protection against human metapneumovirus infections in mice.
abstract: Human metapneumovirus (HMPV) is a paramyxovirus that causes acute respiratory-tract infections in children and adults worldwide. A safe and effective vaccine could decrease the burden of disease associated with this novel pathogen. We engineered HMPV viral-like particles (HMPV-VLPs) derived from retroviral core particles that mimic the properties of the viral surface of two HMPV viruses of either lineage A or B. These VLPs functionally display F and G HMPV surface glycoproteins. When injected in mice, HMPV-VLPs induce strong humoral immune response against both homologous and heterologous strains. Moreover, the induced neutralizing antibodies prevented mortality upon subsequent infection of the lungs with both homologous and heterologous viruses. Upon challenge, viral titers in the lungs of immunized animals were significantly reduced as compared to those of control animals. In conclusion, a HMPV-VLP vaccine that induces cross-protective immunity in mice is a promising approach to prevent HMPV infections.
authors.affiliation: CIRI, International Center for Infectiology Research, EVIR Team, Université de Lyon, Lyon, France.
authors.forename: Camille
authors.initials: C
authors.lastname: Lévy
authors.forename: Laetitia
authors.initials: L
authors.lastname: Aerts
authors.forename: Marie-Ève
authors.initials: MÈ
authors.lastname: Hamelin
authors.forename: Christelle
authors.initials: C
authors.lastname: Granier
authors.forename: Judit
authors.initials: J
authors.lastname: Szécsi
authors.forename: Dimitri
authors.initials: D
authors.lastname: Lavillette
authors.forename: Guy
authors.initials: G
authors.lastname: Boivin
authors.forename: François-Loïc
authors.initials: FL
authors.lastname: Cosset
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology; D002460:Cell Line; D002522:Chlorocebus aethiops; D056738:Cross Protection; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D056724:Immunity, Humoral; D008168:Lung / Q000276:immunology / Q000821:virology; D029121:Metapneumovirus / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D018184:Paramyxoviridae Infections / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D012141:Respiratory Tract Infections / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D058425:Vaccines, Virus-Like Particle / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; C484233:G glycoprotein, human metapneumovirus; D006023:Glycoproteins; D058425:Vaccines, Virus-Like Particle; D014764:Viral Proteins