title: YB-1 stabilizes HIV-1 genomic RNA and enhances viral production.
abstract: HIV-1 utilizes cellular factors for efficient replication. The viral RNA is different from cellular mRNAs in many aspects, and is prone to attacks by cellular RNA quality control systems. To establish effective infection, the virus has evolved multiple mechanisms to protect its RNA. Here, we show that expression of the Y-box binding protein 1 (YB-1) enhanced the production of HIV-1. Downregulation of endogenous YB-1 in producer cells decreased viral production. YB-1 increased viral protein expression by stabilizing HIV-1 RNAs. The stem loop 2 in the HIV-1 RNA packaging signal was mapped to be the YB-1-responsive element. Taken together, these results indicate that YB-1 stabilizes HIV-1 genomic RNA and thereby enhances HIV-1 gene expression and viral production.
authors.affiliation: Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Mu
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Xinlu
authors.initials: X
authors.lastname: Wang
authors.forename: Guangxia
authors.initials: G
authors.lastname: Gao
mesh_terms: D020121:5' Untranslated Regions; D002874:Chromosome Mapping; D015536:Down-Regulation; D057809:HEK293 Cells; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism; D006801:Humans; D055029:Inverted Repeat Sequences; D011485:Protein Binding; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D012367:RNA, Viral / Q000378:metabolism*; D014779:Virus Replication; D051840:Y-Box-Binding Protein 1 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
chemical_list: D020121:5' Untranslated Regions; D034741:RNA, Small Interfering; D012367:RNA, Viral; D051840:Y-Box-Binding Protein 1