title: Reg3G gene expression in regenerating skeletal muscle and corresponding nerve.
abstract: BACKGROUND
  The Reg genes play a major role in the regeneration of various tissues; however, no reports have been published regarding expression of the Reg3G gene in skeletal muscle. In this study we investigated the expression of the Reg3G gene in regeneration of rat skeletal muscle and injured nerves.
  
  
  METHODS
  We used 3 experimental models of muscle and nerve injury. RT-PCR and Western blot analysis were performed for detection of Reg3G in regenerating muscle and nerve.
  
  
  RESULTS
  We found transcriptional activation of the Reg3G gene in the soleus and extensor digitorum longus muscles and in their corresponding nerves after both muscle and nerve injury in different time periods, respectively.
  
  
  CONCLUSIONS
  The results suggest that the Reg3G gene plays a major role in communication between injured axons and muscle and may play a significant role in skeletal muscle and peripheral nerve regeneration.
authors.affiliation: Department of Anatomy, School of Medicine, University of Rijeka, Brace Branchetta 20, 51000, Rijeka, Croatia.
authors.forename: Gordana Starcevic
authors.initials: GS
authors.lastname: Klasan
authors.forename: Danijel
authors.initials: D
authors.lastname: Ivanac
authors.forename: Dubravka Jurisic
authors.initials: DJ
authors.lastname: Erzen
authors.forename: Anne
authors.initials: A
authors.lastname: Picard
authors.forename: Shin
authors.initials: S
authors.lastname: Takasawa
authors.forename: Stanislav
authors.initials: S
authors.lastname: Peharec
authors.forename: Juraj
authors.initials: J
authors.lastname: Arbanas
authors.forename: Dean
authors.initials: D
authors.lastname: Girotto
authors.forename: Romana
authors.initials: R
authors.lastname: Jerkovic
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D037181:Lectins, C-Type / Q000235:genetics / Q000378:metabolism*; D008297:Male; D023421:Models, Animal; D018482:Muscle, Skeletal / Q000293:injuries / Q000294:innervation / Q000378:metabolism*; D009416:Nerve Regeneration / Q000502:physiology*; D000073718:Pancreatitis-Associated Proteins; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration / Q000502:physiology*; D012584:Sciatic Nerve / Q000293:injuries / Q000378:metabolism*; D013997:Time Factors
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D037181:Lectins, C-Type; D000073718:Pancreatitis-Associated Proteins; D012333:RNA, Messenger; C000612145:Reg3g protein, rat
keywords: RT-PCR; Reg3G gene; denervation; muscle regeneration; rat