title: Response of tobacco to the Pseudomonas syringae pv. Tomato DC3000 is mainly dependent on salicylic acid signaling pathway.
abstract: Pseudomonas syringae pv. Tomato DC3000 (Pst DC3000) was the first pathogen to be demonstrated to infect Arabidopsis and to cause disease symptoms in the laboratory setting. However, the defense response to Pst DC3000 was unclear in tobacco. In this report, the expression profiles of twelve defense response-related genes were analyzed after treatment with salicylic acid (SA), jasmonic acid (JA), and pathogen Pst DC3000 by qRT-PCR. According to our results, it could be presented that the genes primarily induced by SA were also induced to higher levels after Pst DC3000 infection. SA accumulation could be induced to a higher level than that of JA after Pst DC3000 infection. In addition, SA could result in hypersensitive response (HR), which did not completely depend on accumulation of reactive oxygen species. These results indicated that tobacco mainly depended on SA signaling pathway rather than on JA signaling pathway in response to Pst DC3000. Further study demonstrated that JA could significantly inhibit the accumulation of SA and the generation of the HR induced by Pst DC3000.
authors.affiliation: State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Guohua
authors.initials: G
authors.lastname: Cai
authors.forename: Shanshan
authors.initials: S
authors.lastname: Jiang
authors.forename: Liping
authors.initials: L
authors.lastname: Sun
authors.forename: Dequan
authors.initials: D
authors.lastname: Li
mesh_terms: D060467:Disease Resistance / Q000235:genetics / Q000276:immunology; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology / Q000382:microbiology*; D044224:Pseudomonas syringae / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D020156:Salicylic Acid / Q000378:metabolism* / Q000494:pharmacology; D015398:Signal Transduction; D014026:Nicotiana / Q000235:genetics / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology*
chemical_list: D017382:Reactive Oxygen Species; D020156:Salicylic Acid