title: Oleic, linoleic and linolenic acids increase ros production by fibroblasts via NADPH oxidase activation.
abstract: The effect of oleic, linoleic and γ-linolenic acids on ROS production by 3T3 Swiss and Rat 1 fibroblasts was investigated. Using lucigenin-amplified chemiluminescence, a dose-dependent increase in extracellular superoxide levels was observed during the treatment of fibroblasts with oleic, linoleic and γ-linolenic acids. ROS production was dependent on the addition of β-NADH or NADPH to the medium. Diphenyleneiodonium inhibited the effect of oleic, linoleic and γ-linolenic acids on fibroblast superoxide release by 79%, 92% and 82%, respectively. Increased levels of p47 (phox) phosphorylation due to fatty acid treatment were detected by Western blotting analyses of fibroblast proteins. Increased p47 (phox) mRNA expression was observed using real-time PCR. The rank order for the fatty acid stimulation of the fibroblast oxidative burst was as follows: γ-linolenic > linoleic > oleic. In conclusion, oleic, linoleic and γ-linolenic acids stimulated ROS production via activation of the NADPH oxidase enzyme complex in fibroblasts.
authors.affiliation: Institute of Physical Activity and Sport Sciences, Cruzeiro do Sul University, São Paulo, Brazil. ehata@usp.br
authors.forename: Elaine
authors.initials: E
authors.lastname: Hatanaka
authors.forename: Alexandre
authors.initials: A
authors.lastname: Dermargos
authors.forename: Aparecida Emiko
authors.initials: AE
authors.lastname: Hirata
authors.forename: Marco Aurélio Ramirez
authors.initials: MA
authors.lastname: Vinolo
authors.forename: Angelo Rafael
authors.initials: AR
authors.lastname: Carpinelli
authors.forename: Philip
authors.initials: P
authors.lastname: Newsholme
authors.forename: Hugo Aguirre
authors.initials: HA
authors.lastname: Armelin
authors.forename: Rui
authors.initials: R
authors.lastname: Curi
mesh_terms: D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D007700:Kinetics; D008042:Linolenic Acids / Q000494:pharmacology*; D051379:Mice; D019255:NADPH Oxidases / Q000235:genetics / Q000378:metabolism*; D019301:Oleic Acid / Q000494:pharmacology*; D010084:Oxidation-Reduction; D010766:Phosphorylation / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013481:Superoxides / Q000378:metabolism
chemical_list: D008042:Linolenic Acids; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D013481:Superoxides; D019301:Oleic Acid; D019255:NADPH Oxidases; C060505:neutrophil cytosolic factor 1