title: Simple and rapid detection of L-Dopa decarboxylase activity using gold nanoparticles.
abstract: We developed a new detection method for L-Dopa decarboxylase (DDC) activity using gold nanoparticles (AuNPs). When L-3,4-dihydroxyphenylalanine (L-Dopa) is decarboxylated to dopamine (DA) by DDC, DA induces the aggregation of AuNPs, and the color of the AuNPs changes from red to blue.
authors.affiliation: BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
authors.forename: So Young
authors.initials: SY
authors.lastname: Park
authors.forename: Dohyoung
authors.initials: D
authors.lastname: Kwon
authors.forename: Hyejung
authors.initials: H
authors.lastname: Mok
authors.forename: Bong Hyun
authors.initials: BH
authors.lastname: Chung
mesh_terms: D003124:Colorimetry; D004296:Dopa Decarboxylase / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism; D057075:Enzyme Assays / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D013997:Time Factors
chemical_list: D006046:Gold; D004296:Dopa Decarboxylase; D004298:Dopamine