title: Androgen-responsive serum response factor target genes regulate prostate cancer cell migration.
abstract: Progression of prostate cancer (CaP) relies on androgen receptor (AR) signaling, but AR-dependent events that underlie the lethal phenotype remain unknown. Recently, an indirect mechanism of androgen action in which effects of AR on CaP cells are mediated by Serum Response Factor (SRF) has been identified. This is the first mode of androgen action to be associated with aggressive CaP and disease recurrence. The manner in which androgen-responsive SRF activity controls aggressive CaP cell behavior is unknown. Here, the contribution of two representative SRF effector genes that are underexpressed, calponin 2 (CNN2), or overexpressed, sidekick-homolog 1 (SDK1), in clinical CaP specimens is studied. AR- and SRF- dependency of CNN2 and SDK1 expression was verified using synthetic and natural androgens, antiandrogens, and small interfering RNAs targeting AR or SRF, and evaluating the kinetics of androgen induction and SRF binding to endogenously and exogenously expressed regulatory gene regions in AR-positive CaP model systems that mimic the transition from androgen-stimulated to castration-recurrent disease. Small interfering RNA-mediated deregulation of CNN2 or SDK1 expression did not affect CaP cell proliferation or apoptosis but had marked effects on CaP cell morphology and actin cytoskeleton organization. Loss of CNN2 induced cellular protrusions and increased CaP cell migration, whereas silencing of SDK1 led to cell rounding and blunted CaP cell migration. Changes in cell migration did not involve epithelial-mesenchymal transition but correlated with altered β1-integrin expression. Taken together, individual androgen-responsive SRF target genes affect CaP cell behavior by modulating cell migration, which may have implications for therapeutic intervention downstream of AR and SRF.
authors.affiliation: Department of Urology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA.
authors.forename: Alissa R
authors.initials: AR
authors.lastname: Verone
authors.forename: Kelly
authors.initials: K
authors.lastname: Duncan
authors.forename: Alejandro
authors.initials: A
authors.lastname: Godoy
authors.forename: Neelu
authors.initials: N
authors.lastname: Yadav
authors.forename: Andrei
authors.initials: A
authors.lastname: Bakin
authors.forename: Shahriar
authors.initials: S
authors.lastname: Koochekpour
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Jin
authors.forename: Hannelore V
authors.initials: HV
authors.lastname: Heemers
mesh_terms: D008841:Actin Cytoskeleton / Q000235:genetics / Q000378:metabolism; D000728:Androgens / Q000235:genetics* / Q000378:metabolism; D017209:Apoptosis / Q000235:genetics; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D048708:Cell Growth Processes / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D018450:Disease Progression; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D006801:Humans; D019012:Integrin beta1 / Q000235:genetics / Q000378:metabolism; D008297:Male; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism; D009919:Orchiectomy; D064129:Prostatic Neoplasms, Castration-Resistant / Q000235:genetics* / Q000378:metabolism / Q000473:pathology*; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism; D026362:Serum Response Factor / Q000235:genetics* / Q000378:metabolism
chemical_list: C410798:AR protein, human; D000728:Androgens; D015815:Cell Adhesion Molecules; D019012:Integrin beta1; D008840:Microfilament Proteins; D011944:Receptors, Androgen; C493091:SDK1 protein, human; C476858:SRF protein, human; D026362:Serum Response Factor