title: Evaluation of anti-apoptotic activity of different dietary antioxidants in renal cell carcinoma against hydrogen peroxide.
abstract: OBJECTIVE
  To evaluate the anti-apoptotic and radical scavenging activities of dietary phenolics, namely ascorbic acid,α-tocopherol acetate, citric acid, salicylic acid, and estimate H2O2-induced apoptosis in renal cell carcinoma cells.
  
  
  METHODS
  The intracellular antioxidant potency of antioxidants was investigated. H2O2-induced apoptosis in RCC-26 was assayed with the following parameters: cell viability (% apoptosis), nucleosomal damage and DNA fragmentation, bcl-2 levels and flow cytometery analysis (ROS production evaluation).
  
  
  RESULTS
  The anticancer properties of antioxidants such as ascorbic acid, α-tocopherol acetate, citric acid, salicylic acid with perdurable responses were investigated. It was observed that these antioxidants had protective effect (anti-apoptotic activity) against hydrogen peroxide (H2O2) in renal cell carcinoma (RCC-26) cell line.
  
  
  CONCLUSIONS
  This study reveals and proves the anticancer properties. However, in cancer cell lines anti-apoptotic activity can indirectly reflect the cancer promoter activity through radicals scavenging, and significantly protect nucleus and bcl-2.
authors.affiliation: Department of Pharmaceutical Sciences, Dr. H. S. Gour University, Sagar, (M.P.), India.
authors.forename: Neeraj K
authors.initials: NK
authors.lastname: Garg
authors.forename: Sharad
authors.initials: S
authors.lastname: Mangal
authors.forename: Tejram
authors.initials: T
authors.lastname: Sahu
authors.forename: Abhinav
authors.initials: A
authors.lastname: Mehta
authors.forename: Suresh P
authors.initials: SP
authors.lastname: Vyas
authors.forename: Rajeev K
authors.initials: RK
authors.lastname: Tyagi
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002292:Carcinoma, Renal Cell; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000032:analysis / Q000378:metabolism; D014815:Vitamins / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D014815:Vitamins; D006861:Hydrogen Peroxide
keywords: Anticancer; Antioxidants; Apoptosis; Hydrogen peroxide; RCC-26; ROS