title: Organosilylated complex [Eu(TTA)3(Bpy-Si)]: a bifunctional moiety for the engeneering of luminescent silica-based nanoparticles for bioimaging.
abstract: A new highly luminescent europium complex with the formula [Eu(TTA)3(Bpy-Si)], where TTA stands for the thenoyltrifluoroacetone, (C4H3S)COCH2COCF3, chelating ligand and Bpy-Si, Bpy-CH2NH(CH2)3Si(OEt)3, is an organosilyldipyridine ligand displaying a triethoxysilyl group as a grafting function has been synthesized and fully characterized. This bifunctional complex has been grafted onto the surface of dense silica nanoparticles (NPs) and on mesoporous silica microparticles as well. The covalent bonding of [Eu(TTA)3(Bpy-Si)] inside uniform Stöber silica nanoparticles was also achieved. The general methodology proposed could be applied to any silica matrix, allowed high grafting ratios that overcome chelate release and the tendency to agglomerate. Luminescent silica-based nanoparticles SiO2-[Eu(TTA)3(Bpy-Si)], with a diameter of 28 ± 2 nm, were successfully tested as a luminescent labels for the imaging of Pseudomonas aeruginosa biofilms. They were also functionalized by a specific monoclonal antibody and subsequently employed for the selective imaging of Escherichia coli bacteria.
authors.affiliation: Centre Interuniversitaire de Recherche et d'Ingénierie des Matériaux, Université de Toulouse, UPS-CNRS 5085, Toulouse, France.
authors.forename: Adriana P
authors.initials: AP
authors.lastname: Duarte
authors.forename: Léïla
authors.initials: L
authors.lastname: Mauline
authors.forename: Marie
authors.initials: M
authors.lastname: Gressier
authors.forename: Jeannette
authors.initials: J
authors.lastname: Dexpert-Ghys
authors.forename: Christine
authors.initials: C
authors.lastname: Roques
authors.forename: José Maurício A
authors.initials: JM
authors.lastname: Caiut
authors.forename: Elenice
authors.initials: E
authors.lastname: Deffune
authors.forename: Danielle C G
authors.initials: DC
authors.lastname: Maia
authors.forename: Iracilda Z
authors.initials: IZ
authors.lastname: Carlos
authors.forename: Antonio A P
authors.initials: AA
authors.lastname: Ferreira
authors.forename: Sidney J L
authors.initials: SJ
authors.lastname: Ribeiro
authors.forename: Marie-Joëlle
authors.initials: MJ
authors.lastname: Menu
mesh_terms: D018441:Biofilms / Q000254:growth & development*; D004926:Escherichia coli / Q000254:growth & development*; D005063:Europium / Q000737:chemistry; D049449:Luminescence; D053758:Nanoparticles / Q000737:chemistry*; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D017646:Organosilicon Compounds / Q000737:chemistry; D010316:Particle Size; D011550:Pseudomonas aeruginosa / Q000254:growth & development*; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D009942:Organometallic Compounds; D017646:Organosilicon Compounds; D005063:Europium; D012822:Silicon Dioxide