title: Overexpression of phyA and appA genes improves soil organic phosphorus utilisation and seed phytase activity in Brassica napus.
abstract: Phytate is the major storage form of organic phosphorus in soils and plant seeds, and phosphorus (P) in this form is unavailable to plants or monogastric animals. In the present study, the phytase genes phyA and appA were introduced into Brassica napus cv Westar with a signal peptide sequence and CaMV 35S promoter, respectively. Three independent transgenic lines, P3 and P11 from phyA and a18 from appA, were selected. The three transgenic lines exhibited significantly higher exuded phytase activity when compared to wild-type (WT) controls. A quartz sand culture experiment demonstrated that transgenic Brassica napus had significantly improved P uptake and plant biomass. A soil culture experiment revealed that seed yields of transgenic lines P11 and a18 increased by 20.9% and 59.9%, respectively, when compared to WT. When phytate was used as the sole P source, P accumulation in seeds increased by 20.6% and 46.9% with respect to WT in P11 and a18, respectively. The P3 line accumulated markedly more P in seeds than WT, while no significant difference was observed in seed yields when phytate was used as the sole P source. Phytase activities in transgenic canola seeds ranged from 1,138 to 1,605 U kg(-1) seeds, while no phytase activity was detected in WT seeds. Moreover, phytic acid content in P11 and a18 seeds was significantly lower than in WT. These results introduce an opportunity for improvement of soil and seed phytate-P bioavailability through genetic manipulation of oilseed rape, thereby increasing plant production and P nutrition for monogastric animals.
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, and Microelement Research Center, Huazhong Agricultural University, Wuhan, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiangsheng
authors.initials: X
authors.lastname: Ye
authors.forename: Guangda
authors.initials: G
authors.lastname: Ding
authors.forename: Fangsen
authors.initials: F
authors.lastname: Xu
mesh_terms: D010832:6-Phytase / Q000096:biosynthesis / Q000235:genetics*; D000135:Acid Phosphatase / Q000096:biosynthesis / Q000235:genetics*; D001234:Aspergillus niger / Q000201:enzymology; D029688:Brassica napus / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000096:biosynthesis / Q000235:genetics*; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics*; D015870:Gene Expression; D010755:Organophosphates / Q000378:metabolism*; D010833:Phytic Acid / Q000378:metabolism; D018520:Plant Shoots / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D012639:Seeds / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D012987:Soil / Q000737:chemistry
chemical_list: D029968:Escherichia coli Proteins; D005656:Fungal Proteins; D010755:Organophosphates; D012987:Soil; D010833:Phytic Acid; D000135:Acid Phosphatase; D010832:6-Phytase; C406788:appA protein, E coli