title: [Free fatty acids induce proliferation of human renal carcinoma 786-O cell line via integrin-linked kinase].
abstract: OBJECTIVE
  To investigate the effects of free fatty acids on cell proliferation and integrin-linked kinase (ILK) expression in human renal carcinoma 786-O cell line.
  
  
  METHODS
  The 786-O cells were exposed to normal medium and different concentrations of oleic acid (OA) carried by de-fatty bovine serum albumin (d-BSA). The MTT assay and the flow cytometry assay were performed respectively for cell proliferation and apoptosis after the treatment with OA for 48 h. The expressions of ILK, Akt and p-Akt were detected by Western blot.
  
  
  RESULTS
  The MTT assay showed that the cell viabilities of 0.05 mmol/L, 0.1 mmol/L and 0.2 mmol/L OA groups were increased gradually, as compared with the blank control (absorbance: 0.657 ± 0.056, 0.682 ± 0.028, 0.718 ± 0.042 vs. 0.495 ± 0.034; all P<0.001). The effects of OA on cells apoptosis were not significant (apoptotic rates: 2.42% ± 0.25% vs. 2.33% ± 0.87% vs. 2.25%± 0.51%, P=0.082). After being treated with OA, the expressions of ILK and p-Akt were increased in 786-O cells.
  
  
  CONCLUSIONS
  The results suggested that free fatty acids could promote the development of renal cell carcinoma via up-regulating ILK/Akt pathway, which may reveal the relations between metabolic disturbance and renal carcinoma to a certain extent.
authors.affiliation: Department of Urology, Peking University People's Hospital, Beijing 100044, China.
authors.forename: Zhen-hua
authors.initials: ZH
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Xie
authors.forename: Yun-bei
authors.initials: YB
authors.lastname: Xiao
authors.forename: Ye-qing
authors.initials: YQ
authors.lastname: Yuan
authors.forename: Xiao-wei
authors.initials: XW
authors.lastname: Zhang
authors.forename: Cai-peng
authors.initials: CP
authors.lastname: Qin
authors.forename: Yi-chang
authors.initials: YC
authors.lastname: Hao
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
authors.forename: Xiao-feng
authors.initials: XF
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D005230:Fatty Acids, Nonesterified / Q000494:pharmacology*; D006801:Humans; D007680:Kidney Neoplasms / Q000201:enzymology / Q000473:pathology*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D015398:Signal Transduction; D015854:Up-Regulation; D000099200:Scaffold Protein ILK
chemical_list: D005230:Fatty Acids, Nonesterified; D017346:Protein Serine-Threonine Kinases; D051057:Proto-Oncogene Proteins c-akt; D000099200:Scaffold Protein ILK