title: The expression of mucin genes and the presence of mucin gene products in the equine endometrium.
abstract: In the equine reproductive tract, little is known about mucin gene expression and the role of mucins in barrier function and host-cell interaction. The aims of the study were to identify equine orthologs of mammalian mucin genes using available equine sequence data, to profile expression of equine orthologous mucin genes in the endometrium using reverse transcriptase polymerase chain reaction (RT-PCR), to determine spatial expression patterns of mucin genes using in situ hybridisation, and to confirm the presence of mucin gene products using Western blotting and equine-specific mucin antibodies during oestrus and dioestrus. While the mucin gene expression pattern in equine endometrium is similar to that of other mammals, several mucins appear to be uniquely expressed in this tissue (eqMUC3B, 7, 18, and 20) and one is hormonally regulated (eqMUC3B).
authors.affiliation: School of Veterinary Medicine, Veterinary Sciences Centre, University College Dublin, Belfield, Dublin 4, Ireland.
authors.forename: Eva
authors.initials: E
authors.lastname: Maischberger
authors.forename: Carolyn A
authors.initials: CA
authors.lastname: Cummins
authors.forename: Eamonn
authors.initials: E
authors.lastname: Fitzpatrick
authors.forename: Mary E
authors.initials: ME
authors.lastname: Gallagher
authors.forename: Sheila
authors.initials: S
authors.lastname: Worrall
authors.forename: Karine
authors.initials: K
authors.lastname: Rousseau
authors.forename: David J
authors.initials: DJ
authors.lastname: Thornton
authors.forename: Wim G
authors.initials: WG
authors.lastname: Meijer
authors.forename: Raúl
authors.initials: R
authors.lastname: Miranda-Casoluengo
authors.forename: Vivienne E
authors.initials: VE
authors.lastname: Duggan
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Carrington
authors.forename: Jane A
authors.initials: JA
authors.lastname: Irwin
authors.forename: Colm J
authors.initials: CJ
authors.lastname: Reid
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D004717:Endometrium / Q000378:metabolism*; D030762:Estrous Cycle / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006736:Horses / Q000235:genetics / Q000378:metabolism*; D017403:In Situ Hybridization / Q000662:veterinary; D009077:Mucins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D012313:RNA / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D009077:Mucins; D012313:RNA