title: Aggregated gas molecules: toxic to protein?
abstract: The biological toxicity of high levels of breathing gases has been known for centuries, but the mechanism remains elusive. Earlier work mainly focused on the influences of dispersed gas molecules dissolved in water on biomolecules. However, recent studies confirmed the existence of aggregated gas molecules at the water-solid interface. In this paper, we have investigated the binding preference of aggregated gas molecules on proteins with molecular dynamics simulations, using nitrogen (N₂) gas and the Src-homology 3 (SH3) domain as the model system. Aggregated N₂ molecules were strongly bound by the active sites of the SH3 domain, which could impair the activity of the protein. In contrast, dispersed N₂ molecules did not specifically interact with the SH3 domain. These observations extend our understanding of the possible toxicity of aggregates of gas molecules in the function of proteins.
authors.affiliation: Division of Interfacial Water and Laboratory of Physical Biology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, PO Box 800-204, Shanghai 201800, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Zhang
authors.forename: Guanghong
authors.initials: G
authors.lastname: Zuo
authors.forename: Jixiu
authors.initials: J
authors.lastname: Chen
authors.forename: Yi
authors.initials: Y
authors.lastname: Gao
authors.forename: Haiping
authors.initials: H
authors.lastname: Fang
mesh_terms: D001665:Binding Sites; D003198:Computer Simulation; D005740:Gases / Q000737:chemistry*; D008956:Models, Chemical; D008958:Models, Molecular; D009584:Nitrogen / Q000737:chemistry*; D011485:Protein Binding; D011487:Protein Conformation; D011489:Protein Denaturation; D018909:src Homology Domains; D019061:src-Family Kinases / Q000737:chemistry* / Q000648:ultrastructure*
chemical_list: D005740:Gases; D019061:src-Family Kinases; D009584:Nitrogen