title: Specific lipidome signatures in central nervous system from methionine-restricted mice.
abstract: Membrane lipid composition is an important correlate of the rate of aging of animals. Dietary methionine restriction (MetR) increases lifespan in rodents. The underlying mechanisms have not been elucidated but could include changes in tissue lipidomes. In this work, we demonstrate that 80% MetR in mice induces marked changes in the brain, spinal cord, and liver lipidomes. Further, at least 50% of the lipids changed are common in the brain and spinal cord but not in the liver, suggesting a nervous system-specific lipidomic profile of MetR. The differentially expressed lipids includes (a) specific phospholipid species, which could reflect adaptive membrane responses, (b) sphingolipids, which could lead to changes in ceramide signaling pathways, and (c) the physiologically redox-relevant ubiquinone 9, indicating adaptations in phase II antioxidant response metabolism. In addition, specific oxidation products derived from cholesterol, phosphatidylcholine, and phosphatidylethanolamine were significantly decreased in the brain, spinal cord, and liver from MetR mice. These results demonstrate the importance of adaptive responses of membrane lipids leading to increased stress resistance as a major mechanistic contributor to the lowered rate of aging in MetR mice.
authors.affiliation: Department of Experimental Medicine, Faculty of Medicine, University of Lleida -IRBLleida, Lleida, Spain.
authors.forename: Mariona
authors.initials: M
authors.lastname: Jové
authors.forename: Victòria
authors.initials: V
authors.lastname: Ayala
authors.forename: Omar
authors.initials: O
authors.lastname: Ramírez-Núñez
authors.forename: Alba
authors.initials: A
authors.lastname: Naudí
authors.forename: Rosanna
authors.initials: R
authors.lastname: Cabré
authors.forename: Corinne M
authors.initials: CM
authors.lastname: Spickett
authors.forename: Manuel
authors.initials: M
authors.lastname: Portero-Otín
authors.forename: Reinald
authors.initials: R
authors.lastname: Pamplona
mesh_terms: D000222:Adaptation, Physiological; D000375:Aging / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002784:Cholesterol / Q000302:isolation & purification / Q000378:metabolism; D005260:Female; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism; D008136:Longevity; D008297:Male; D008715:Methionine / Q000172:deficiency*; D051379:Mice; D008810:Mice, Inbred C57BL; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010713:Phosphatidylcholines / Q000302:isolation & purification / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000302:isolation & purification / Q000378:metabolism; D017382:Reactive Oxygen Species; D013116:Spinal Cord / Q000378:metabolism; D014451:Ubiquinone / Q000378:metabolism
chemical_list: D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D017382:Reactive Oxygen Species; D014451:Ubiquinone; C483858:phosphatidylethanolamine; D002784:Cholesterol; D008715:Methionine; C030571:ubiquinone 9