title: Melanoidins isolated from heated potato fiber (Potex) affect human colon cancer cells growth via modulation of cell cycle and proliferation regulatory proteins.
abstract: Melanoidins are brown, nitrogen containing, high molecular weight end products of Maillard reaction with poorly established activity towards tumor cells. The goal of present study was to verify whether both heated potato fiber Potex extract (180°C for 2h) and melanoidins isolated from the extract exerts growth-inhibiting activity in human colon cancer cells in vitro. The cells of LS180 colon cancer cell line were tested upon treatment with roasted potato fiber extract (AM4) as well as with high (HMW) and low (LMW) molecular weight fractions isolated from the extract, since both may be regarded as/or contain melanoidins. The tested compounds at concentration of 1000 μg/ml reduced cell growth down to 45%, 69% and 54%, respectively. Furthermore, deregulated ERK1/2 signaling was revealed upon treatment. Moreover, multiple alternations in cell cycle regulators activity were found (i.e. cyclinD1, cyclin-dependent kinase 4 and 6, p21, p27, p53, pRb) leading to cell cycle cessation in G0 phase. Importantly, LMW compounds revealed markedly stronger potential to alter specific molecular targets comparing to HMW compounds. Summarizing, the results emphasize that both high and low molecular weight melanoidins contribute to antiproliferative activity of heated potato fiber in LS180 colon cancer cells in vitro.
authors.affiliation: Department of Medical Biology, Institute of Agricultural Medicine, Jaczewskiego 2, 20-090 Lublin, Poland. ewa.langner@gmail.com
authors.forename: Ewa
authors.initials: E
authors.lastname: Langner
authors.forename: Fernando M
authors.initials: FM
authors.lastname: Nunes
authors.forename: Piotr
authors.initials: P
authors.lastname: Pożarowski
authors.forename: Martyna
authors.initials: M
authors.lastname: Kandefer-Szerszeń
authors.forename: Stefan G
authors.initials: SG
authors.lastname: Pierzynowski
authors.forename: Wojciech
authors.initials: W
authors.lastname: Rzeski
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology*; D002241:Carbohydrates / Q000032:analysis; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology*; D019938:Cyclin D1 / Q000378:metabolism; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics / Q000378:metabolism; D003593:Cytoplasm / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D020868:Gene Silencing; D006801:Humans; D019950:Mitogen-Activated Protein Kinase 1 / Q000235:genetics; D048052:Mitogen-Activated Protein Kinase 3 / Q000235:genetics; D008970:Molecular Weight; D010936:Plant Extracts / Q000737:chemistry; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D021381:Protein Transport / Q000187:drug effects; D016192:Resting Phase, Cell Cycle / Q000187:drug effects; D011198:Solanum tuberosum / Q000737:chemistry*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D000972:Antineoplastic Agents, Phytogenic; C530104:CCND1 protein, human; C493956:CDKN1A protein, human; D002241:Carbohydrates; D050759:Cyclin-Dependent Kinase Inhibitor p21; D010936:Plant Extracts; D011108:Polymers; D016159:Tumor Suppressor Protein p53; C011908:melanoidin polymers; D019938:Cyclin D1; C535150:MAPK1 protein, human; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3