title: miRNA let-7e modulates the Wnt pathway and early nephrogenic markers in mouse embryonic stem cell differentiation.
abstract: This study indicates that embryonic stem cells [ESCs] cultured with retinoic acid and activin A significantly upregulate the miRNA let-7e. This specific miRNA modulates the Wnt pathway and the expression of early nephrogenic markers under these differentiation conditions. The differentiation markers WT1, Pax2 and Wnt4 were downregulated when miRNA let-7e was silenced, thus indicating the role of miRNA let-7e in the differentiation process. PKCβ, GSK3β phosphorylation (GSK3β(P)) and β-catenin expression was reduced in differentiated cells and reversed by miRNA let-7e silencing. Addition of a PKCβ inhibitor to the miRNA let-7e silenced cells abolished let-7e-derived effects in differentiation markers, and reversed the increase in GSK3β(P) and β-catenin, thus indicating that miRNA let-7e is involved in differentiation via the modulation of GSK3β phosphorylation and β-catenin production.
authors.affiliation: Departament of Experimental Pathology, Instituto de Investigaciones Biomédicas de Barcelona (IIBB-CSIC, IDIBAPS), Barcelona, Spain. vinasmjose@gmail.com
authors.forename: Jose Luis
authors.initials: JL
authors.lastname: Viñas
authors.forename: Marina
authors.initials: M
authors.lastname: Ventayol
authors.forename: Bernhard
authors.initials: B
authors.lastname: Brüne
authors.forename: Michaela
authors.initials: M
authors.lastname: Jung
authors.forename: Anna
authors.initials: A
authors.lastname: Sola
authors.forename: Felip
authors.initials: F
authors.lastname: Pi
authors.forename: Chrysoula
authors.initials: C
authors.lastname: Mastora
authors.forename: Georgina
authors.initials: G
authors.lastname: Hotter
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D002454:Cell Differentiation; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects; D020868:Gene Silencing; D007668:Kidney / Q000196:embryology / Q000378:metabolism*; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014212:Tretinoin / Q000494:pharmacology; D060449:Wnt Signaling Pathway
chemical_list: D015415:Biomarkers; D035683:MicroRNAs; C583909:mirnlet7e microRNA, mouse; D014212:Tretinoin