title: Stabilization of membrane bound ATPases and lipid peroxidation by carotenoids from Chlorococcum humicola in Benzo(a)pyrene induced toxicity.
abstract: OBJECTIVE
  To identify the alteration of the membrane potential and the effect of carotenoid extracts from Chlorococcum humicola (C. humicola) on membrane bound ATPases and lipid peroxidation.
  
  
  METHODS
  The total carotenoids were extracted from C. humicola. Four groups of Swiss albino mice were treated as control, Benzo(a)pyrene [B(a)P], total carotenoids, B(a)P + total carotenoids respectively for a period of 60 days. Membrane lipid peroxidation and ATPases (Total ATPases, Ca(2+)- ATPases, Mg(2+) - ATPases, Na(+)K(+) - ATPase) were determined in lung, liver and erythrocyte samples.
  
  
  RESULTS
  The activity of total ATPase was found to be significantly increased in the B(a)P treated liver and lung tissue. Erythrocyte membrane also showed higher ATPase activity which was significantly reverted on total carotenoid treatment.
  
  
  CONCLUSIONS
  It can be concluded that the changes in membrane potential favour the functional deterioration of physiological system. The overall findings demonstrates that the animals post treated with carotenoid extract from C. humicola may maintains the alterations in membrane bound ATPase and lipid peroxidation in tissues against the carcinogenic chemical and hence aid in establishing the membrane potential action. Therefore C. humicola can be further extended to exploits its possible application for various health benefits as neutraceuticals and food additives.
authors.affiliation: Bharathiar University, Coimbatore, Tamilnadu, India.
authors.forename: S
authors.initials: S
authors.lastname: Bhagavathy
authors.forename: P
authors.initials: P
authors.lastname: Sumathi
mesh_terms: D000251:Adenosine Triphosphatases / Q000187:drug effects; D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D002338:Carotenoids / Q000494:pharmacology; D000460:Chlorophyta / Q000737:chemistry*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004910:Erythrocyte Membrane / Q000187:drug effects; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008168:Lung / Q000187:drug effects* / Q000473:pathology; D051379:Mice; D008517:Phytotherapy / Q000379:methods*; D028321:Plant Preparations / Q000494:pharmacology*
chemical_list: D004791:Enzyme Inhibitors; D028321:Plant Preparations; D001564:Benzo(a)pyrene; D002338:Carotenoids; D000251:Adenosine Triphosphatases
keywords: Benzo(a)pyrene; Ca2+ ATPase; Carotenoids; Lipid peroxidation; Mg2+ ATPase; Na+/K+ ATPase; Total ATPase