title: Expression and clinical significance of CD73 and hypoxia-inducible factor-1α in gastric carcinoma.
abstract: OBJECTIVE
  To investigate the expression of CD73 and hypoxia-inducible factor-1α (HIF-1α) in human gastric carcinoma, and explore their clinical significance and prognostic value.
  
  
  METHODS
  CD73 and HIF-1α expressions were detected by immunohistochemistry in consecutive sections of tissue samples from 68 gastric carcinoma patients. The peritumor tissues 2 cm away from the tumor were obtained and served as controls. The presence of CD73 and HIF-1α was analyzed by immunohistochemistry using the Envision technique.
  
  
  RESULTS
  CD73 and HIF-1α expressions in gastric carcinoma were significantly higher than those in gastric mucosal tissues as control (P < 0.001) and showed a close correlation (Spearman r = 0.390, P = 0.001). Overexpression of CD73 was positively correlated with differentiation of tumor (P = 0.000), histopathology (P = 0.041), depth of invasion (P < 0.001), nodal status (P = 0.003), metastasis (P = 0.013), and the American Joint Committee on Cancer (AJCC) stage (P < 0.001). High expression of HIF-1α was positively correlated with tumor diameter (P = 0.031), depth of invasion (P = 0.022), and AJCC stage (P = 0.035). The overall survival rate was low in the patients with high expression of CD73 (P < 0.001). Moreover, CD73+/HIF-1α+ patients had the worst prognosis (P < 0.001). CD73 expression was proven to be an independent predictor for patients with gastric carcinoma by both multivariate Cox regression analysis (P = 0.021) and receiver operating characteristic curves (P = 0.001).
  
  
  CONCLUSIONS
  CD73 expression correlates closely with HIF-1α expression in gastric carcinoma. CD73 could be an independent prognostic indicator for gastric carcinoma.
authors.affiliation: Department of Medical Oncology, Nanjing General Hospital of Nanjing Military Command, Medical School of Nanjing University, Nanjing 210002, Jiangsu Province, China.
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Lu
authors.forename: Yi-Tian
authors.initials: YT
authors.lastname: Chen
authors.forename: Bing
authors.initials: B
authors.lastname: Feng
authors.forename: Xiao-Bei
authors.initials: XB
authors.lastname: Mao
authors.forename: Bo
authors.initials: B
authors.lastname: Yu
authors.forename: Xiao-Yuan
authors.initials: XY
authors.lastname: Chu
mesh_terms: D015720:5'-Nucleotidase / Q000378:metabolism*; D000328:Adult; D002277:Carcinoma / Q000378:metabolism*; D002681:China / Q000453:epidemiology; D005260:Female; D058851:GPI-Linked Proteins / Q000378:metabolism; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D013270:Stomach / Q000473:pathology; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D055815:Young Adult
chemical_list: D058851:GPI-Linked Proteins; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D015720:5'-Nucleotidase; C548241:NT5E protein, human
keywords: CD73; Gastric carcinoma; Hypoxia-inducible factor-1α; Immunohistochemistry; Prognosis