title: LDL receptor and its family members serve as the cellular receptors for vesicular stomatitis virus.
abstract: Vesicular stomatitis virus (VSV) exhibits a remarkably robust and pantropic infectivity, mediated by its coat protein, VSV-G. Using this property, recombinant forms of VSV and VSV-G-pseudotyped viral vectors are being developed for gene therapy, vaccination, and viral oncolysis and are extensively used for gene transduction in vivo and in vitro. The broad tropism of VSV suggests that it enters cells through a highly ubiquitous receptor, whose identity has so far remained elusive. Here we show that the LDL receptor (LDLR) serves as the major entry port of VSV and of VSV-G-pseudotyped lentiviral vectors in human and mouse cells, whereas other LDLR family members serve as alternative receptors. The widespread expression of LDLR family members accounts for the pantropism of VSV and for the broad applicability of VSV-G-pseudotyped viral vectors for gene transduction.
authors.affiliation: Department of Molecular Genetics, The Weizmann Institute of Science, Rehovot 76100, Israel.
authors.forename: Danit
authors.initials: D
authors.lastname: Finkelshtein
authors.forename: Ariel
authors.initials: A
authors.lastname: Werman
authors.forename: Daniela
authors.initials: D
authors.lastname: Novick
authors.forename: Sara
authors.initials: S
authors.lastname: Barak
authors.forename: Menachem
authors.initials: M
authors.lastname: Rubinstein
mesh_terms: D000818:Animals; D002417:Cattle; D004705:Endocytosis; D005347:Fibroblasts / Q000166:cytology / Q000821:virology; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D051379:Mice; D011485:Protein Binding; D011973:Receptors, LDL / Q000378:metabolism*; D011991:Receptors, Virus / Q000378:metabolism*; D012995:Solubility; D014161:Transduction, Genetic; D014721:Vesicular stomatitis Indiana virus / Q000378:metabolism* / Q000472:pathogenicity; D053586:Virus Internalization
chemical_list: D011973:Receptors, LDL; D011991:Receptors, Virus