title: Expression and regulation of Runx3 in mouse uterus during the peri-implantation period.
abstract: The aim of this study was to investigate the differential expression and regulation of Runt-related transcription factor 3 (Runx3) in mouse uterus during early pregnancy and its regulation by steroid hormones using in situ hybridization. There was a low level of the Runx3 mRNA expression in the mouse uterus on days 1-4 of pregnancy. On day 5 when embryo implanted, Runx3 mRNA signal was obviously observed in the stromal cells surrounding the implanting blastocyst. From day 6 to 8 of pregnancy, Runx3 mRNA was highly expressed in the decidual cells and mesometrial decidual beds. Similarly, Runx3 mRNA was strongly expressed in decidualized cells under artificial decidualization. Compared with the delayed uterus, a high level of Runx3 mRNA signal was detected in the uterus with activated implantation. In the ovariectomized mouse uterus, estrogen could induce the expression of Runx3, while progesterone had no effects. These results suggest that Runx3 may play an important role during mouse implantation and decidualization. Estrogen can induce the expression of Runx3 in the ovariectomized mouse uterus.
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun, 130062, People's Republic of China.
authors.forename: Zhi-Kun
authors.initials: ZK
authors.lastname: Bai
authors.forename: Bin
authors.initials: B
authors.lastname: Guo
authors.forename: Xue-Chao
authors.initials: XC
authors.lastname: Tian
authors.forename: Dang-Dang
authors.initials: DD
authors.lastname: Li
authors.forename: Shou-Tang
authors.initials: ST
authors.lastname: Wang
authors.forename: Hang
authors.initials: H
authors.lastname: Cao
authors.forename: Qu-Yuan
authors.initials: QY
authors.lastname: Wang
authors.forename: Zhan-Peng
authors.initials: ZP
authors.lastname: Yue
mesh_terms: D000818:Animals; D050677:Core Binding Factor Alpha 3 Subunit / Q000235:genetics* / Q000378:metabolism; D003656:Decidua / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010064:Embryo Implantation / Q000235:genetics*; D004967:Estrogens / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation; D005865:Gestational Age; D017403:In Situ Hybridization; D051379:Mice; D010052:Ovariectomy; D011247:Pregnancy; D011374:Progesterone / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D017154:Stromal Cells
chemical_list: D050677:Core Binding Factor Alpha 3 Subunit; D004967:Estrogens; D012333:RNA, Messenger; C493730:Runx3 protein, mouse; D011374:Progesterone