title: Antimicrobial potential of Actinomycetes species isolated from marine environment.
abstract: OBJECTIVE
  To evaluate the antimicrobial activity of Actinomycetes species isolated from marine environment.
  
  
  METHODS
  Twenty one strains of Actinomycetes were isolated from samples of Royapuram, Muttukadu, Mahabalipuram sea shores and Adyar estuary. Preliminary screening was done using cross-streak method against two gram-positive and eight gram-negative bacteria. The most potent strains C11 and C12 were selected from which antibacterial substances were extracted. The antibacterial activities of the extracts were performed using Kirby-Bauer disc diffusion method. Molecular identification of those isolates was done.
  
  
  RESULTS
  All those twenty one isolates were active against at least one of the test organisms. Morphological characters were recorded. C11 showed activity against Staphylococcus species (13.0±0.5 mm), Vibrio harveyi (11.0±0.2 mm), Pseudomonas species (12.0±0.3 mm). C12 showed activity against Staphylococcus species (16.0±0.4 mm), Bacillus subtilis (11.0±0.2 mm), Vibrio harveyi (9.0±0.1 mm), Pseudomonas species (10.0±0.2 mm). 16S rRNA pattern strongly suggested that C11 and C12 strains were Streptomyces species.
  
  
  CONCLUSIONS
  The results of the present investigation reveal that the marine Actinomycetes from coastal environment are the potent source of novel antibiotics. Isolation, characterization and study of Actinomycetes can be useful in discovery of novel species of Actinomycetes.
authors.affiliation: PG and Research Department of Microbiology, Mohamed Sathak College of Arts & Science, Sholinganallur, Chennai.
authors.forename: S
authors.initials: S
authors.lastname: Valli
authors.forename: Sugasini S
authors.initials: SS
authors.lastname: Suvathi
authors.forename: O S
authors.initials: OS
authors.lastname: Aysha
authors.forename: P
authors.initials: P
authors.lastname: Nirmala
authors.forename: Kumar P
authors.initials: KP
authors.lastname: Vinoth
authors.forename: A
authors.initials: A
authors.lastname: Reena
mesh_terms: D039903:Actinobacteria / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification* / Q000502:physiology*; D000890:Anti-Infective Agents / Q000302:isolation & purification*; D000898:Antibiosis; D059001:Aquatic Organisms / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification* / Q000502:physiology*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D000890:Anti-Infective Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
keywords: 16S rRNA; Antibacterial substance; Antimicrobial activity; Cross-streak method; Kirby-Bauer disc diffusion method; Marine Actinomycetes; Molecular identification; Morphological character; Streptomyces