title: Human olfactory receptors: recombinant expression in the baculovirus/Sf9 insect cell system, functional characterization, and odorant identification.
abstract: Cell surface expression of recombinant olfactory receptors (ORs) is a major limitation in characterizing their functional nature. We have shown that the recombinant expression of a human OR, OR 17-210, in the baculovirus/Sf9 insect cell system allows this protein to be expressed at the cell surface. We used Ca(2+) imaging to demonstrate that recombinant OR 17-210 produces cellular activities upon odorant stimulation with ketones. Furthermore, this expression and functional system has been used to show that the preincubation of Human Odorant Binding Protein 2A decrease the calcium response of OR 17-210 following stimulation by acetophenone and beta ionone.
authors.affiliation: Centre de Recherche en Neurobiologie et Neurophysiologie de Marseille, CRN2M, CNRS UMR6231, Université Paul Cézanne, Marseille, France.
authors.forename: Valéry
authors.initials: V
authors.lastname: Matarazzo
authors.forename: Catherine
authors.initials: C
authors.lastname: Ronin
mesh_terms: D000098:Acetophenones / Q000378:metabolism; D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D002118:Calcium / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D054834:Lipocalins / Q000378:metabolism; D018613:Microscopy, Confocal; D045792:Norisoprenoids / Q000378:metabolism; D009812:Odorants; D018035:Receptors, Odorant / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D061987:Sf9 Cells; D018411:Spodoptera / Q000166:cytology
chemical_list: D000098:Acetophenones; D054834:Lipocalins; D045792:Norisoprenoids; C509994:OBP2A protein, human; D018035:Receptors, Odorant; D011994:Recombinant Proteins; C008157:beta-ionone; C038699:acetophenone; D002118:Calcium