title: Toward Homogeneous Erythropoietin: Application of Metal Free Dethiylation in the Chemical Synthesis of the Ala79-Arg166 Glycopeptide Domain.
abstract: We describe herein the assembly of hEPO(79-166), a key glycopeptide segment en route to erythropoietin, in minimally protected form. Key to the success of this synthetic endeavor was the application of our two-step cysteine-free native chemical ligation strategy, by which we achieved formal ligation at alanine and proline residues through the use of an N-terminal amino acid surrogate presenting a readily removable thiol functionality.
authors.affiliation: Laboratory for Bioorganic Chemistry, Sloan-Kettering Institute for Cancer Research, 1275 York Avenue, New York, NY 10065, USA.
authors.forename: Suwei
authors.initials: S
authors.lastname: Dong
authors.forename: Shiying
authors.initials: S
authors.lastname: Shang
authors.forename: Zhongping
authors.initials: Z
authors.lastname: Tan
authors.forename: Samuel J
authors.initials: SJ
authors.lastname: Danishefsky