title: Antibacterial activity of Lawsonia inermis Linn (Henna) against Pseudomonas aeruginosa.
abstract: OBJECTIVE
  To investigate the antibacterial activity of henna (Lawsonia inermis Linn) obtained from different regions of Oman against a wide array of micro-organisms.
  
  
  METHODS
  Fresh henna samples were obtained from different regions of Oman as leaves and seeds. 100 g fresh and dry leaves and 50 g of fresh and dry seeds were separately soaked in 500 mL of ethanol for three days, respectively, with frequent agitation. The mixture was filtered, and the crude extract was collected. The crude extract was then heated, at 48 °C in a water bath to evaporate its liquid content. The dry crude henna extract was then tested for its antibacterial activity using well-diffusion antibiotic susceptibility technique. Henna extracts were investigated for their antibacterial activity at different concentrations against a wide array of different micro-organisms including a laboratory standard bacterial strain of Pseudomonas aeruginosa (NCTC 10662) (P. aeruginosa) and eleven fresh clinical isolates of P. aeruginosa obtained from patients attending the Sultan Qaboos University Hospital (SQUH). 2-Hydroxy-p-Nathoqinone-Tech (2-HPNT, MW=174.16, C10H6O3) was included as control (at 50% concentration) along with the henna samples tested.
  
  
  RESULTS
  Henna samples demonstrated antibacterial activity against all isolates but the highest susceptibility was against P. aeruginosa with henna samples obtained from Al-sharqyia region.
  
  
  CONCLUSIONS
  Omani henna from Al-sharqyia region demonstrates high in vitro anti-P. aeruginosa activity compared with many henna samples from different regions of Oman.
authors.affiliation: Department of Human & Clinical Anatomy, College of Medicine and Health Sciences, Sultan Qaboos University, P.O. Box: 35, Code: 123.
authors.forename: O
authors.initials: O
authors.lastname: Habbal
authors.forename: S S
authors.initials: SS
authors.lastname: Hasson
authors.forename: A H
authors.initials: AH
authors.lastname: El-Hag
authors.forename: Z
authors.initials: Z
authors.lastname: Al-Mahrooqi
authors.forename: N
authors.initials: N
authors.lastname: Al-Hashmi
authors.forename: Z
authors.initials: Z
authors.lastname: Al-Bimani
authors.forename: M S
authors.initials: MS
authors.lastname: Al-Balushi
authors.forename: A A
authors.initials: AA
authors.lastname: Al-Jabri
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D031564:Lawsonia Plant / Q000737:chemistry*; D008826:Microbial Sensitivity Tests; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts
keywords: Antibacterial activity; Antibiotic susceptibility; Bacterial strain; Clinical isolate; Crude extract; Henna; Lawsonia inermis Linn; Micro-organism; Oman; Pseudomonas aeruginosa