title: Effect of platelet-rich plasma on the biologic activity of the human rotator-cuff fibroblasts: A controlled in vitro study.
abstract: To assess the in vitro effect of platelet-rich plasma (PRP) on biological activity of the human rotator cuff fibroblasts and to describe the optimal dose-response to maximize cellular stimulation while reducing potential risk. Rotator cuff (RC) fibroblasts of n = 6 patients (mean age of 65.2 years) undergoing arthroscopic cuff tear reconstruction were cultured in vitro for 21 days and stimulated with PRP in three different concentrations (1-, 5-, and 10-fold). Samples were obtained for DNA and GAG measurement at 1, 7, 14, and 21 days. The biological outcomes were regressed on the PRP concentration. The application of PRP significantly influenced the fibroblast proliferation and activity of the human rotator cuff with elevated glycosaminoglycan (GAG) and DNA levels. The dosage of PRP had the significantly highest impact on this proliferation using a onefold or fivefold application. PRP has a significant effect on fibroblast proliferation of the human rotator cuff in vitro with an optimal benefit using a onefold or fivefold PRP concentration. This study justifies further in vivo investigations using PRP at the human rotator cuff.
authors.affiliation: Department of Orthopaedic Surgery, Medical University Graz, Graz, Austria. patricksadoghi@gmx.at
authors.forename: Patrick
authors.initials: P
authors.lastname: Sadoghi
authors.forename: Birgit
authors.initials: B
authors.lastname: Lohberger
authors.forename: Birgit
authors.initials: B
authors.lastname: Aigner
authors.forename: Heike
authors.initials: H
authors.lastname: Kaltenegger
authors.forename: Jörg
authors.initials: J
authors.lastname: Friesenbichler
authors.forename: Matthias
authors.initials: M
authors.lastname: Wolf
authors.forename: Tarek
authors.initials: T
authors.lastname: Sununu
authors.forename: Andreas
authors.initials: A
authors.lastname: Leithner
authors.forename: Patrick
authors.initials: P
authors.lastname: Vavken
mesh_terms: D000368:Aged; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism; D006801:Humans; D008875:Middle Aged; D053657:Platelet-Rich Plasma / Q000502:physiology*; D017006:Rotator Cuff / Q000166:cytology*
chemical_list: D006025:Glycosaminoglycans; D004247:DNA