title: Weissellicin L, a novel bacteriocin from sian-sianzih-isolated Weissella hellenica 4-7.
abstract: OBJECTIVE
  To identify and characterize novel bacteriocins from Weissella hellenica 4-7.
  
  
  RESULTS
  Weissella hellenica 4-7, isolated from the traditional Taiwanese fermented food sian-sianzih (fermented clams), was previously found to produce a bacteriocin active against Listeria monocytogenes and some other Gram-positive bacteria. Bacteriocin activity decreased slightly after autoclaving (121°C for 15 min), but was inactivated by protease K and trypsin. Mass spectrometry analysis revealed the bacteriocin mass to be approximately 3205·6 Da. N-terminal amino acid sequencing yielded a partial sequence, NH2 -KGFLSWASKATSWLVGP, by Edman degradation. The obtained partial sequence showed high homology with leucocin B-TA33a; however, at least two different residues were observed. No identical peptide or protein was found, and this peptide was therefore considered to be a novel bacteriocin produced by W. hellenica 4-7 and termed weissellicin L.
  
  
  CONCLUSIONS
  The findings obtained in the current study suggest a novel bacteriocin produced by W. hellenica 4-7.
  
  
  CONCLUSIONS
  Bacteriocins from Weissella remain rare, and this study is the second report of a bacteriocin produced by W. hellenica.
authors.affiliation: Department of Biotechnology, Ming Chuan University, Gui-Shan Township, Taoyuan County, Taiwan.
authors.forename: K-H
authors.initials: KH
authors.lastname: Leong
authors.forename: Y-S
authors.initials: YS
authors.lastname: Chen
authors.forename: Y-H
authors.initials: YH
authors.lastname: Lin
authors.forename: S-F
authors.initials: SF
authors.lastname: Pan
authors.forename: B
authors.initials: B
authors.lastname: Yu
authors.forename: H-C
authors.initials: HC
authors.lastname: Wu
authors.forename: F
authors.initials: F
authors.lastname: Yanagida
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D001430:Bacteriocins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D049872:Bivalvia / Q000382:microbiology; D008969:Molecular Sequence Data; D017747:Seafood / Q000382:microbiology; D058836:Weissella / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins; C071726:leucocin A-UAL 187