title: Core-shell nanoparticle controlled hATSCs neurogenesis for neuropathic pain therapy.
abstract: A stem cell-based strategy for tissue engineering in regenerative medicine is crucial to produce and effective therapeutic replacement of injured or damaged tissues. This type of therapeutic replacement requires interaction with the cells and tissues via the incorporation of a beneficial physical microenvironment and cellular biochemical signals. Recently, we studied a cell-function modifying factor, core-shell nanoparticles consisting of an SPIO (superparamagnetic iron oxide) core covered with a photonic ZnO shell for human adipose tissue-derived stem cells (hATSCs) that regulate various cellular functions: self-renewal, neurogenesis, and dedifferentiation. We proposed an alternative method of stem cell culture that focuses on the use of Zn++ Finger nanoparticles for stem cell expansion and transdifferentiation modulation in vitro and in in vivo spinal cord injury models. Our study showed that treating hATSC cultures with nanoscale particles could lead to active cell proliferation and self-renewal and could promote nuclear Dicer-regulation of several functional molecules, Oct4 and Glutathione peroxidase 3 (GPx3), and the abundance of specific functional proteins that have been observed using biochemical analysis. These biochemical changes in hATSCs induced the functional development of multiple differentiation potencies such as β-cells and neural cells; specifically, the ability to differentiation into GABA-secreting cells was significantly improved in in vitro- and in vivo-induced animal lesions with significantly improved therapeutic modality.
authors.affiliation: Laboratory of Stem Cell Biology, Department of Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
authors.forename: Jee In
authors.initials: JI
authors.lastname: Choi
authors.forename: Hyun Tae
authors.initials: HT
authors.lastname: Cho
authors.forename: Min Ki
authors.initials: MK
authors.lastname: Jee
authors.forename: Soo Kyung
authors.initials: SK
authors.lastname: Kang
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D019070:Cell Lineage; D002467:Cell Nucleus / Q000378:metabolism; D054338:Cell Transdifferentiation; D016922:Cellular Senescence; D053487:DEAD-box RNA Helicases / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005290:Ferric Compounds / Q000737:chemistry; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D008813:Mice, Inbred ICR; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009437:Neuralgia / Q000628:therapy*; D055495:Neurogenesis; D043244:Ribonuclease III / Q000378:metabolism; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology*; D015034:Zinc Oxide / Q000737:chemistry
chemical_list: D005290:Ferric Compounds; C000499:ferric oxide; C408087:DICER1 protein, human; D043244:Ribonuclease III; D053487:DEAD-box RNA Helicases; D015034:Zinc Oxide