title: A methodology to infer gene networks from spatial patterns of expression--an application to fluorescence in situ hybridization images.
abstract: The proper functional development of a multicellular organism depends on an intricate network of interacting genes that are expressed in accurate temporal and spatial patterns across different tissues. Complex inhibitory and excitatory interactions among genes control the territorial differences that explain specialized cell fates, embryo polarization and tissues architecture in metazoans. Given the nature of the regulatory gene networks, similarity of expression patterns can identify genes with similar roles. The inference and analysis of the gene interaction networks through complex network tools can reveal important aspects of the biological system modeled. Here we suggest an image analysis pipeline to quantify co-localization patterns in in situ hybridization images of Drosophila embryos and, based on these patterns, infer gene networks. We analyze the spatial dispersion of the gene expression and show the gene interaction networks for different developmental stages. Our results suggest that the inference of developmental networks based on spatial expression data is biologically relevant and represents a potential tool for the understanding of animal development.
authors.affiliation: Instituto de Física de São Carlos - Universidade de São Paulo, Av. Trabalhador São-carlense, 400, São Carlos-SP, Brasil. mocampiteli@gmail.com
authors.forename: Monica Guimarães
authors.initials: MG
authors.lastname: Campiteli
authors.forename: Cesar Henrique
authors.initials: CH
authors.lastname: Comin
authors.forename: Luciano da Fontoura
authors.initials: Lda F
authors.lastname: Costa
authors.forename: M Madan
authors.initials: MM
authors.lastname: Babu
authors.forename: Roberto Marcondes
authors.initials: RM
authors.lastname: Cesar
mesh_terms: D000818:Animals; D004330:Drosophila / Q000196:embryology / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods*; D018507:Gene Expression Regulation, Developmental; D053263:Gene Regulatory Networks; D007091:Image Processing, Computer-Assisted / Q000379:methods; D017404:In Situ Hybridization, Fluorescence