title: TGF-β1 gene-engineered mesenchymal stem cells induce rat cartilage regeneration using nonviral gene vector.
abstract: This study evaluated the potential of utilizing transfected pTGFβ-1 gene-engineered rat mesenchymal stem cells (MSCs) using nonviral vector to promote cartilage regeneration. Pullulan-spermine was used as the nonviral gene vector and gelatin sponge was used as the scaffold. MSCs were engineered with TGF-β1 gene with either the three-dimensional (3D) reverse transfection system or the two-dimensional (2D) conventional transfection system. For the 3D reverse transfection system, pullulan-spermine/pTGF-β1 gene complexes were immobilized to the gelatin sponge, followed by the seeding of MSCs. Pullulan-spermine/pTGF-β1 gene complexes were delivered to MSCs cultured in the plate to perform the 2D conventional transfection system, and then MSCs were seeded to the gelatin sponge. Then, TGF-β1 gene-transfected MSC seeded gelatin sponge was implanted to the full-thickness cartilage defect. Compared with the control group, both groups of TGF-β1 gene-engineered MSCs improved cartilage regeneration through optical observation and histology staining. So, with pullulan-spermine as the nonviral vector, TGF-β1-gene engineered MSCs can induce cartilage regeneration in vivo.
authors.affiliation: Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, People's Republic of China.
authors.forename: Cai-Xia
authors.initials: CX
authors.lastname: He
authors.forename: Tian-Yuan
authors.initials: TY
authors.lastname: Zhang
authors.forename: Pei-Hong
authors.initials: PH
authors.lastname: Miao
authors.forename: Zhong-Jie
authors.initials: ZJ
authors.lastname: Hu
authors.forename: Min
authors.initials: M
authors.lastname: Han
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Tabata
authors.forename: Yu-Lan
authors.initials: YL
authors.lastname: Hu
authors.forename: Jian-Qing
authors.initials: JQ
authors.lastname: Gao
mesh_terms: D000818:Animals; D002356:Cartilage / Q000166:cytology* / Q000378:metabolism; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics*; D005936:Glucans / Q000235:genetics / Q000378:metabolism; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D008855:Microscopy, Electron, Scanning; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration / Q000235:genetics*; D013096:Spermine / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000235:genetics*
chemical_list: D005936:Glucans; D053773:Transforming Growth Factor beta1; D013096:Spermine; C009109:pullulan