title: Profiling of selected indigenous rice (Oryza sativa L.) landraces of Rarh Bengal in relation to osmotic stress tolerance.
abstract: A total of ten rare indigenous rice landraces of West Bengal were screened for germination potential and seedling growth under varying concentrations of sodium chloride (NaCl) and polyethylene glycol (PEG) solutions as osmotic stress inducing agents. Among the studied rice landraces Kelas and Bhut Moori showed highest degree of tolerance to induced osmotic stresses. Proline content of the studied lines was also determined. Genetic relationship among the studied rice landraces was assessed with 22 previously reported osmotic stress tolerance linked Simple Sequence Repeat (SSR) markers. The identified allelic variants in form of amplified products size (molecular weight) for each SSR marker were documented to find out allele mining set for the linked markers of the studied genotypes in relation to osmotic stress tolerance. A Microsatellite Panel was constructed for the different allelic forms (size of amplified products) of each used marker. Among 22 SSR markers, ten showed unique alleles in form of single specific amplified product for the studied four genotypes which can be used for varietal identification. Genetic relationship among the studied rice lines was determined and a dendrogram was constructed to reveal their genetic inter-relationship. Polymorphism Information Content (PIC) for each used marker was also calculated for the studied rice lines.
authors.affiliation: Centre for Biotechnology, Visva-Bharati, Santiniketan, 731235 West Bengal India.
authors.forename: Joydip
authors.initials: J
authors.lastname: Karmakar
authors.forename: Rajib
authors.initials: R
authors.lastname: Roychowdhury
authors.forename: Rup Kumar
authors.initials: RK
authors.lastname: Kar
authors.forename: Debal
authors.initials: D
authors.lastname: Deb
authors.forename: Narottam
authors.initials: N
authors.lastname: Dey
keywords: Indigenous rice landraces; Microsatellite panel; Osmotic stress; Polymorphism information content; Simple sequence repeat