title: Retinoic acid inhibits adipogenesis via activation of Wnt signaling pathway in 3T3-L1 preadipocytes.
abstract: Although retinoic acid (RA) is well known to inhibit the differentiation of 3T3-L1 cells into adipocytes both in vivo and in vitro, its molecular mechanism is not fully understood. In this report, we investigate the inhibitory mechanism of adipocyte differentiation by RA in 3T3-L1 cells. Because both RA and Wnt are known to inhibit adipogenesis at a common step involving the inhibition of PPAR-γ expression, we focused on the crosstalk between these two signaling pathways. We found that RA treatment resulted in a dramatic inhibition of adipogenesis, especially at an early phase of differentiation, and led to increased β-catenin protein expression. Moreover, RA enhances the transcriptional activity of β-catenin as well as Wnt gene expression during adipogenesis. Taken together, the present study demonstrated that Wnt/β-catenin signaling may be associated with the RA-induced suppression of adipogenesis and may cooperatively inhibit adipocyte differentiation.
authors.affiliation: Department of Medical System Engineering, Gwangju Institute of Science and Technology, 123 Cheomdan-gwagiro, Buk-gu, Gwangju 500-712, Republic of Korea.
authors.forename: Dong Min
authors.initials: DM
authors.lastname: Kim
authors.forename: Hye-Ryung
authors.initials: HR
authors.lastname: Choi
authors.forename: Anna
authors.initials: A
authors.lastname: Park
authors.forename: Sang-Mo
authors.initials: SM
authors.lastname: Shin
authors.forename: Kwang-Hee
authors.initials: KH
authors.lastname: Bae
authors.forename: Sang Chul
authors.initials: SC
authors.lastname: Lee
authors.forename: Il-Chul
authors.initials: IC
authors.lastname: Kim
authors.forename: Won Kon
authors.initials: WK
authors.lastname: Kim
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D001483:Base Sequence; D002454:Cell Differentiation; D002839:Chromans / Q000494:pharmacology; D017931:DNA Primers; D051379:Mice; D047495:PPAR gamma / Q000187:drug effects; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction; D045162:Thiazolidinediones / Q000494:pharmacology; D014212:Tretinoin / Q000494:pharmacology*; D000077288:Troglitazone; D051153:Wnt Proteins / Q000378:metabolism*; D051176:beta Catenin / Q000378:metabolism
chemical_list: D002839:Chromans; D017931:DNA Primers; D047495:PPAR gamma; D045162:Thiazolidinediones; D051153:Wnt Proteins; D051176:beta Catenin; D014212:Tretinoin; D000077288:Troglitazone