title: DICO, a novel nonaromatic B-ring flavonoid, induces G2/M cell cycle arrest and apoptosis in human hepatoma cells.
abstract: DICO was a novel nonaromatic B-ring flavonoid obtained from Macrothelypteris torresiana. In the present work, we investigated the antitumor activity and the antineoplastic mechanism of DICO. Our study showed that DICO inhibited the growth of HepG2 cells in dose and time-dependent manners. As well as DICO induced G2/M cell cycle arrest and apoptosis via a ROS-mediated mitochondrial pathway. Western blot assay demonstrated that DICO decreased Bcl-2 level and induced Bax translocation to cause cytochrome c release. Subsequently, caspase-9 and caspase-3 were activated. Meanwhile, the alterations of cyclin A and B1, p-CDK1 and p-cdc25c levels were also observed in response to DICO treatment. Taken together, DICO displayed a significant antitumor effect through G2/M cell cycle arrest and apoptosis induction, which suggested DICO might have therapeutic potential against tumors.
authors.affiliation: Beijing Institute of Biotechnology, Beijing 100000, China.
authors.forename: Daonian
authors.initials: D
authors.lastname: Zhou
authors.forename: Anhua
authors.initials: A
authors.lastname: Wei
authors.forename: Cheng
authors.initials: C
authors.lastname: Cao
authors.forename: Jinlan
authors.initials: J
authors.lastname: Ruan
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D016203:CDC2 Protein Kinase / Q000378:metabolism; D019925:Cyclin A / Q000378:metabolism; D056744:Cyclin B1 / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D029624:Ferns / Q000737:chemistry; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D059565:G2 Phase Cell Cycle Checkpoints / Q000187:drug effects*; D056945:Hep G2 Cells / Q000187:drug effects / Q000473:pathology; D006801:Humans; D059566:M Phase Cell Cycle Checkpoints / Q000187:drug effects*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000378:metabolism; D020687:cdc25 Phosphatases / Q000378:metabolism
chemical_list: C585838:5,7-dihydroxy-2-(1,2-isopropyldioxy-4-oxo-cyclohex-5-enyl)-chromen-4-one; D000972:Antineoplastic Agents, Phytogenic; C494826:BAX protein, human; C534699:CCNB1 protein, human; D019925:Cyclin A; D056744:Cyclin B1; D005419:Flavonoids; D017382:Reactive Oxygen Species; D051028:bcl-2-Associated X Protein; D016203:CDC2 Protein Kinase; C517009:CDC25C protein, human; D020687:cdc25 Phosphatases