title: MicroRNA-92b regulates the development of intermediate cortical progenitors in embryonic mouse brain.
abstract: Cerebral cortical neurons arise from radial glia (direct neurogenesis) or from intermediate progenitors (indirect neurogenesis); intriguingly, the sizes of intermediate progenitor populations and the cortices they generate correlate across species. The generation of intermediate progenitors is regulated by the transcription factor Tbr2, whose expression marks these cells. We investigated how this mechanism might be controlled. We found that acute blockade of mature microRNA biosynthesis in murine cortical progenitors caused a rapid cell autonomous increase in numbers of Tbr2-expressing cells. Acute microRNA-92b (miR-92b) gain of function caused rapid reductions in numbers of Tbr2-expressing cells and proliferating intermediate progenitors. Acute miR-92b loss of function had opposite effects. These findings indicate that miR-92b limits the production of intermediate cortical progenitors.
authors.affiliation: Centre for Integrative Physiology, University of Edinburgh, Edinburgh EH8 9XD, United Kingdom. tomasz.j.nowakowski@gmail.com
authors.forename: Tomasz Jan
authors.initials: TJ
authors.lastname: Nowakowski
authors.forename: Vassiliki
authors.initials: V
authors.lastname: Fotaki
authors.forename: Andrew
authors.initials: A
authors.lastname: Pollock
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.forename: Thomas
authors.initials: T
authors.lastname: Pratt
authors.forename: David J
authors.initials: DJ
authors.lastname: Price
mesh_terms: D000818:Animals; D001973:Bromodeoxyuridine; D002540:Cerebral Cortex / Q000166:cytology*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008156:Luciferases; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D058953:Neural Stem Cells / Q000502:physiology*; D055495:Neurogenesis / Q000235:genetics / Q000502:physiology*; D009474:Neurons / Q000166:cytology*; D060888:Real-Time Polymerase Chain Reaction; D020825:T-Box Domain Proteins / Q000378:metabolism
chemical_list: C491198:Eomes protein, mouse; D035683:MicroRNAs; C541873:Mirn92 microRNA, mouse; D020825:T-Box Domain Proteins; D008156:Luciferases; D001973:Bromodeoxyuridine