title: Resonance light scattering determination of uranyl based on labeled DNAzyme-gold nanoparticle system.
abstract: A resonance light scattering (RLS) method has been developed using a uranyl (UO2(2+)) specific DNAzyme and gold nanoparticles (AuNPs). In this strategy, the cleavage of the substrate strand (SDNA) of DNAzyme results in releasing a shorter duplex in the presence of UO2(2+), leading to the aggregation of AuNPs and the increase of RLS intensity. The response signals linearly correlated with the concentration of UO2(2+) over the range of 1.36×10(-8)-1.50×10(-7) mol L(-1). The limit of detection (LOD) is 4.09×10(-9) mol L(-1). The method has excellent selectivity and higher sensitivity. It could provide a promising potential for the detection of metal ions, and be benefit to extend the application of RLS method.
authors.affiliation: College of Public Health, University of South China, Hengyang 421001, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: Lin-Fei
authors.initials: LF
authors.lastname: Shi
authors.forename: Yong-Sheng
authors.initials: YS
authors.lastname: Wang
authors.forename: Hui-Xian
authors.initials: HX
authors.lastname: Yang
authors.forename: Jin-Hua
authors.initials: JH
authors.lastname: Xue
authors.forename: Lu
authors.initials: L
authors.lastname: Liu
authors.forename: Yong-Song
authors.initials: YS
authors.lastname: Wang
authors.forename: Ji-Cheng
authors.initials: JC
authors.lastname: Yin
authors.forename: Jia-Cheng
authors.initials: JC
authors.lastname: Wang
mesh_terms: D002413:Cations, Divalent / Q000032:analysis / Q000378:metabolism; D021881:DNA, Catalytic / Q000737:chemistry* / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D008027:Light; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry*; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence / Q000379:methods; D017974:Uranium Compounds / Q000032:analysis* / Q000378:metabolism
chemical_list: D002413:Cations, Divalent; D021881:DNA, Catalytic; D017974:Uranium Compounds; D006046:Gold; C012597:uranium dioxide