title: Effect of OSW-1 on microRNA expression profiles of hepatoma cells and functions of novel microRNAs.
abstract: 3β,16β,17α-trihydroxycholest-5-en-22-one 16-O-(2-O-4-methoxybenzoyl-β-D-xylopyranosyl)-(1→3)- (2-O-acet​yl-α-L-arabinopyranoside) (OSW-1) is a member of the cholestane saponin family, which was first isolated from the bulbs of Ornithogalum saundersiae and previously reported to be cytotoxic against several types of malignant cells. However, its antitumor mechanism remains unclear. Therefore, we investigated microRNA (miRNA) expression profiles in order to explore the antitumor activities of OSW-1. Furthermore, following study of differentially expressed miRNAs, the function of novel miRNAs and OSW-1 was determined using known miRNAs and anticarcinogens. The present study demonstrated that treatment with OSW-1 leads to the upregulation and downregulation of a large set of tumor-related miRNAs, including miR-299, miR-1908, miR-125b, miR-187a, miR-1275, hav1-miR-H6-3p, miR-181, miR-210, miR-483, miR-126, miR-208 and others. Notably, miR-141, miR-142, miR-200C and miR-1275 were found to be upregulated by OSW-1 and doxorubicine, as compared with doxorubicine alone. Additionally, the expression fold-change of miR-142-3P was ~58 times higher than its expression with a different treatment. These miRNAs are linked to cancer, including proliferation, differentiation, apoptosis, cell adhesion, migration, polarity and epithelial to mesenchymal transition (EMT).
authors.affiliation: Department of Hepatopancreatobiliary Surgery, The Affiliated Hospital of Yanbian University, Yanji, Jilin 133000, P.R. China.
authors.forename: Ji-Chun
authors.initials: JC
authors.lastname: Jin
authors.forename: Xing-Lin
authors.initials: XL
authors.lastname: Jin
authors.forename: Xian
authors.initials: X
authors.lastname: Zhang
authors.forename: Ying-Shi
authors.initials: YS
authors.lastname: Piao
authors.forename: Shuang-Ping
authors.initials: SP
authors.lastname: Liu
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002783:Cholestenones / Q000737:chemistry / Q000494:pharmacology*; D015536:Down-Regulation; D004317:Doxorubicin / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology; D035683:MicroRNAs / Q000378:metabolism*; D031442:Ornithogalum / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D012503:Saponins / Q000737:chemistry / Q000494:pharmacology*; D059467:Transcriptome / Q000187:drug effects*; D015854:Up-Regulation
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D002783:Cholestenones; C555159:MIRN142 microRNA, human; D035683:MicroRNAs; D012503:Saponins; C106408:OSW 1; D004317:Doxorubicin