title: Bone marrow stromal cell transdifferentiation into oligodendrocyte-like cells using triiodothyronine as a inducer with expression of platelet-derived growth factor α as a maturity marker.
abstract: The present study investigated the functional maturity of oligodendrocyte derived from rat bone marrow stromal cells (BMSC).
  
  
  
  The BMSC were isolated from female Sprague-Dawley rats and evaluated for different markers, such as fibronectin, CD106, CD90, Oct-4 and CD45. Transdifferentiation of OLC from BMSC was obtained by exposing the BMSC to DMSO and 1 µM all-trans-retinoic acid during the pre-induction stage and then induced by heregulin (HRG), platelet-derived growth factor AA (PDGFR-alpha), fibroblast growth factor and T3. The neuroprogenitor cells (NPC) were evaluated for nestin, neurofilament 68, neurofilament 160 and glial fibrillary acidic protein gene expression using immunocytochemistry. The OLC were assessed by immunocytochemistry for O4, oligo2, O1 and MBP marker and gene expression of PDGFR-alpha was examined by RT-PCR.
  
  
  
  Our results showed that the fibronectin, CD106, CD90, CD45 and Oct-4 were expressed after the fourth passage. Also, the yield of OLC differentiation was about 71% when using the O1, O4 and oligo2 markers. Likewise, the expression of PDGFR-alpha in pre-oligodendrocytes was noticed, while MBP expression was detected in oligodendrocyte after 6 days of the induction.
  
  
  
  The conclusion of the study showed that BMSC can be induced to transdifferentiate into mature OLC.
authors.affiliation: Dept. of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Hojjat-Allah
authors.initials: HA
authors.lastname: Abbaszadeh
authors.affiliation: Dept. of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.|Shefa Neurosciences Research Center, Khatam Al-Anbia Hospital, Tehran, Iran.
authors.forename: Taki
authors.initials: T
authors.lastname: Tiraihi
authors.affiliation: Dept. of Anatomical Sciences, Shahed University, Tehran, Iran.
authors.forename: Ali Reza
authors.initials: AR
authors.lastname: Delshad
authors.affiliation: Dept. of Genetics, Faculty of Basic Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Majid
authors.initials: M
authors.lastname: Saghedi Zadeh
authors.affiliation: Shefa Neurosciences Research Center, Khatam Al-Anbia Hospital, Tehran, Iran.
authors.forename: Taher
authors.initials: T
authors.lastname: Taheri
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D054338:Cell Transdifferentiation / Q000187:drug effects*; D005260:Female; D007150:Immunohistochemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D009836:Oligodendroglia / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000235:genetics / Q000378:metabolism; D014284:Triiodothyronine / Q000494:pharmacology*
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D015415:Biomarkers; D009419:Nerve Tissue Proteins; D050814:Octamer Transcription Factor-3; D010982:Platelet-Derived Growth Factor; D020796:Receptor, Platelet-Derived Growth Factor alpha; D014284:Triiodothyronine; C075278:platelet-derived growth factor A; C093564:Neurogenic differentiation factor 1
keywords: Bone marrow stromal cell; Triiodothyronine; Platelet-derived growth factor α