title: Effect of scraping therapy on interleukin-1 in serum of rats with lumbar disc herniation.
abstract: OBJECTIVE
  To explore the effect of scraping therapy on the Interleukin-1 (IL-1) levels of rats with lumbar disc herniation (LDH).
  
  
  METHODS
  Fifty male rats were devided into a blank group (A), a sham operation group (B), a model group (C), a scraping group (D), and a drug group (E). The rats in the group B were treated with sham operation, and groups C, D and E were made into the LDH model by operation. After operation, group C were treated with no interventions, D were given scraping and E were fed with azathioprine. Then the IL-1 levels of different groups were detected by enzyme-linked immuno sorbent assay method. And the transplanted coccygeal vertebra discs were observed by pathological section.
  
  
  RESULTS
  The IL-1 levels in the groups C, D, and E were significantly higher than those in the groups A and B (all P < 0.01), which proved the operation was successful. The IL-1 levels in the groups D and E at different periods had statistical significance (F = 414.158, P < 0.01). The treatment periods and interventions have interation (F = 46.613, P < 0.01). Multiple comparison results showed that the IL-1 levels in the groups D and E was significantly lower than that in the group C (P < 0.01), while the IL-1 levels between the groups D and E had no statistical significance (P > 0.05). Moreover, pathological section indicated that immuno-inflammatory response was hardly found in coccygeal vertebra discs in the groups A and B, while local immuno-inflammatory responses of the groups D and E were much lighter than that of the group C.
  
  
  CONCLUSIONS
  Scraping therapy could inhibit the immuno-inflammatory responses in the rats with LDH caused by transplantation of autologous nucleus pulposus.
authors.affiliation: Nursing College, Nanjing University of Chinese Medicine, Nanjing 210023, China.
authors.forename: Rongrong
authors.initials: R
authors.lastname: Jiang
authors.forename: Guihua
authors.initials: G
authors.lastname: Xu
authors.forename: Hua
authors.initials: H
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Tao
authors.initials: T
authors.lastname: Yang
authors.forename: Yang
authors.initials: Y
authors.lastname: Guo
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D006801:Humans; D007375:Interleukin-1 / Q000097:blood*; D007405:Intervertebral Disc Displacement / Q000097:blood / Q000276:immunology / Q000628:therapy*; D008297:Male; D008516:Medicine, Chinese Traditional / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D007375:Interleukin-1