title: Proteomic analysis of Ascaridia galli. Identification of immunoreactive proteins in naturally and experimentally infected hens.
abstract: Ascaridia galli, intestinal parasite of domestic fowl, is responsible of economic losses in avian exploitations. However, molecular mechanisms that govern avian ascaridiasis remain largely unknown. The aim of the present work was to identify proteins of A. galli recognized by the immune system of naturally and experimentally infected hens, using two-dimensional electrophoresis (2-DE) and mass spectrometry (MS). Sixteen immunoreactive proteins of A. galli were identified. These proteins are mainly related to different metabolic processes, cell motility and binding activities. The timing evolution of this recognition pattern was studied using serum samples from experimentally infected hens, allowing us to observe an early recognition of many of these antigens. Many of them were isoforms from lipid and plasminogen-binding proteins. Moreover, plasminogen-binding activity has been related in other parasites with the facilitation of intra-organic migration, which represents an important fact in avian ascaridiasis. This work represents the first proteomic study of A. galli and could contribute to explain some aspects of parasite/host relationships of avian ascaridiasis.
authors.affiliation: Laboratory of Parasitology, Faculty of Pharmacy and Institute of Biomedical Research of Salamanca (IBSAL), University of Salamanca, 37007 Salamanca, Spain. jglez@usal.es
authors.forename: Javier
authors.initials: J
authors.lastname: González-Miguel
authors.forename: Cristina
authors.initials: C
authors.lastname: Marcos-Atxutegi
authors.forename: Roberta Bottari
authors.initials: RB
authors.lastname: de Castello
authors.forename: Sara
authors.initials: S
authors.lastname: Carpani
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Morchón
authors.forename: Fernando
authors.initials: F
authors.lastname: Simón
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D001197:Ascaridia / Q000235:genetics / Q000378:metabolism*; D001198:Ascaridiasis / Q000276:immunology / Q000378:metabolism / Q000469:parasitology / Q000662:veterinary*; D002645:Chickens; D005260:Female; D011201:Poultry Diseases / Q000276:immunology / Q000378:metabolism / Q000469:parasitology*; D040901:Proteomics
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth
keywords: Ascaridi galli; Hens; Mass spectrometry; Soluble proteome