title: Analysis of Staphylococcal cassette chromosome mec in BD GeneOhm MRSA assay-negative strains.
abstract: The BD GeneOhm MRSA assay could identify methicillin-resistant Staphylococcus aureus (MRSA) strains at a high ratio (97.8%). Analysis of 11 assay-negative MRSA strains suggested that insertion of non-mec staphylococcal cassette chromosome elements (SCCs) downstream of orfX, and carriage of SCCmecs with a left extremity that cannot be detected by the kit, might lead to their being given an incorrect negative status.
authors.affiliation: Department of Infection Control Science, Graduate School of Medicine, Juntendo University, Tokyo, Japan.
authors.forename: Meng
authors.initials: M
authors.lastname: Zhang
authors.forename: Teruyo
authors.initials: T
authors.lastname: Ito
authors.forename: Shanshuang
authors.initials: S
authors.lastname: Li
authors.forename: Shigeki
authors.initials: S
authors.lastname: Misawa
authors.forename: Shigemi
authors.initials: S
authors.lastname: Kondo
authors.forename: Takashi
authors.initials: T
authors.lastname: Miida
authors.forename: Akimichi
authors.initials: A
authors.lastname: Ohsaka
authors.forename: Keiichi
authors.initials: K
authors.lastname: Hiramatsu
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D005188:False Negative Reactions; D006801:Humans; D016106:Methicillin Resistance / Q000235:genetics*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics* / Q000302:isolation & purification*; D008826:Microbial Sensitivity Tests; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D013203:Staphylococcal Infections / Q000175:diagnosis* / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial