title: Effect of conditioning on the increase of heart rate and body temperature provoked by handling in the mouse.
abstract: To assess the effect of procedures on animal welfare, various physiological parameters, such as body weight, hormone levels in plasma and/or urine, heart rate (HR), blood pressure and body temperature (BT), can be used. When measuring physiological parameters with techniques involving restraint of the animals, the results must be interpreted with caution, since restraint itself may have an effect on those parameters. Radio-telemetry, using an implantable transmitter, provides a way to obtain more accurate and reliable physiological measurements from freely moving animals in their own environment. In this study, we have used radio-telemetry to investigate the influence of conditioning on the increase of HR and BT as provoked by handling of mice. It was found that, after a conditioning period of 12 days, the increase of HR due to handling was significantly reduced.
authors.affiliation: Department of Health, Safety and the Environment, Free University, Amsterdam, The Netherlands. k.kramer@dienst.vu.nl
authors.forename: Klaas
authors.initials: K
authors.lastname: Kramer
authors.forename: Heleen
authors.initials: H
authors.lastname: van de Weerd
authors.forename: Anne
authors.initials: A
authors.lastname: Mulder
authors.forename: Caroline
authors.initials: C
authors.lastname: Van Heijningen
authors.forename: Vera
authors.initials: V
authors.lastname: Baumans
authors.forename: René
authors.initials: R
authors.lastname: Remie
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Voss
authors.forename: Bert F M
authors.initials: BF
authors.lastname: van Zutphen
mesh_terms: D000818:Animals; D000830:Animals, Laboratory; D001831:Body Temperature; D006339:Heart Rate; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013312:Stress, Physiological