title: The role of AUX1 gene and auxin content to the branching phenotype of Kenaf (Hibiscus cannabinus L.).
abstract: The objectives of this research were to identify auxin gene, AUX1, and to determine the plant auxin content and their role in conferring branching on Kenaf. PCR analysis using AUX1 primer capable to amplify the DNA of non branching (KR11) and branching kenaf mutant, resulting in 800 bp PCR product. The sequence of the PCR product showed high degree of homology with the sequence of AUX1 gene of other plants in the NCBI GenBank database, confirming kenaf possession of the gene AUX1. However, some variation on the DNA sequence was found between branching and non branching phenotype indicated allele differences of the same gene which were responsible for the variation in the type of branching. Identification of auxin content in the roots, apical shoot, and axillary branches using spectrophotometry method showed that the branching plant has higher auxin content in the apical shoot compared to the content in the branches. This indicate that AUX1 controls the formation of branches by controlling either the content of auxin in the apical shoot and branches, or the ratio of auxin content in the shoot and branches.
authors.affiliation: Laboratory of Molecular Biology, Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl. Veteran, Malang, Indonesia.
authors.forename: Estri L
authors.initials: EL
authors.lastname: Arumingtyas
authors.forename: R
authors.initials: R
authors.lastname: Mastuti
authors.forename: S
authors.initials: S
authors.lastname: Indriyani
keywords: AUX1; Hibiscus cannabinus; Malvaceae; PCR; auxin; branching