title: Multi-generational effects of rice harboring Bph15 on brown planthopper, Nilaparvata lugens.
abstract: BACKGROUND
  The brown planthopper (BPH), Nilaparvata lugens, is one of the most devastating rice pests in Asia. Resistant cultivars are an effective way of managing BPH. Bph15 is a BPH resistance gene and has been introgressed into rice variety Minghui 63 (MH63). The multi-generational effects of rice line MH63::15 (harboring Bph15) on BPH were investigated and compared with its parental line MH63.
  
  
  RESULTS
  U-test analysis indicated that, over seven generations, the developmental duration of BPH nymphs was significantly prolonged by MH63::15. The results of a two-way analysis indicated that, over seven generations, MH63::15 had significant negative effects on the hatchability, emergence rate, copulation rate, weight of adults and fecundity of BPH, but no significant effects on the survival rate of nymphs or female ratio of BPH. In addition, the development of ovary was significantly retarded by MH63::15, and the expression of oogenesis genes were either down-regulated (three genes) or up-regulated (one genes) by MH63::15 compared with MH63.
  
  
  CONCLUSIONS
  After being reared continuously on MH63::15 for seven generations, most of the life parameters of BPH were negatively affected by MH63::15, especially fecundity and ovary development. These results indicate that MH63::15 rice has potential for use in the control of BPH.
authors.affiliation: Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Keke
authors.initials: K
authors.lastname: Shang
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.forename: Tingru
authors.initials: T
authors.lastname: Jiang
authors.forename: Dingbang
authors.initials: D
authors.lastname: Hu
authors.forename: Hongxia
authors.initials: H
authors.lastname: Hua
mesh_terms: D000818:Animals; D049628:Body Size; D003307:Copulation; D005260:Female; D005298:Fertility; D006430:Hemiptera / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D008297:Male; D009758:Nymph / Q000254:growth & development; D009866:Oogenesis / Q000235:genetics; D012275:Oryza; D010053:Ovary / Q000254:growth & development; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012744:Sex Ratio; D015996:Survival Rate
chemical_list: D012333:RNA, Messenger
keywords: Bph15; Nilaparvata Lugens; multi-generational; rice