title: Antibacterial constituents of three Cameroonian medicinal plants: Garcinia nobilis, Oricia suaveolens and Balsamocitrus camerunensis.
abstract: BACKGROUND
  Multidrug resistance is a worrying cause of treatment failure in bacterial infections. The search of bioactive constituents from medicinal plants against multidrug resistant (MDR) bacteria has significantly evolved in the two last decades. In the present study, twenty-two compounds (three terpenoids, eleven phenolics and eight alkaloids) isolated from three Cameroonian medicinal plants, namely Garcinia nobilis, Oricia suaveolens and Balsamocitrus camerunensis, as well as the crude extracts were tested for their antibacterial activities against Mycobacterium tuberculosis and Gram-negative bacteria amongst which were MDR active efflux pumps expressing phenotypes.
  
  
  METHODS
  The microplate alamar blue assay (MABA) and the broth microdilution methods were used to determine the minimal inhibitory concentrations (MIC) and minimal bactericidal concentrations (MBC) of the studied samples.
  
  
  RESULTS
  The results of the MIC determinations indicate that, the best crude extract was that from G. nobilis (GNB), its inhibitory effects being noted against 12 of the 14 tested bacteria. The extract of GNB also exhibited better anti-tuberculosis (MIC of 128 μg/ml M. tuberculosis against ATCC 27294 strain) and antibacterial (MIC of 64 μg/ml against Escherichia coli ATCC10536) activities compared to the extracts of O. suaveolens and B. camerunensis. Interestingly, 4-prenyl-2-(3,7-dimethyl-2,6-octadienyl)-1,3,5,8-tetrahydroxyxanthone (2), isolated from the most active extract GNB, also showed the best activity amongst compounds, inhibiting the growth of all the fourteen tested microorganisms. The lowest MIC value obtained with compound 2 was 8 μg/ml against M. tuberculosis ATCC 27294 and M. tuberculosis clinical MTCS2 strains. Other compounds showed selective activities with 11 of the 14 tested bacteria being sensitive to the xanthone, morusignin I (5) and the alkaloid, kokusaginine (13).
  
  
  CONCLUSIONS
  The results of the present investigation provide evidence that the crude extract from G. nobilis, O. suaveolens and B. camerunensis as well as some of their compounds, and mostly compound 2 (isolated from G. nobilis,) could be considered as interesting natural antibacterial products.
authors.affiliation: Department of Organic Chemistry, Faculty of Science, University of Yaoundé 1, Yaoundé, Cameroon.
authors.forename: Hugues
authors.initials: H
authors.lastname: Fouotsa
authors.forename: Armelle T
authors.initials: AT
authors.lastname: Mbaveng
authors.forename: Celine D
authors.initials: CD
authors.lastname: Mbazoa
authors.forename: Augustin E
authors.initials: AE
authors.lastname: Nkengfack
authors.forename: Shaheen
authors.initials: S
authors.lastname: Farzana
authors.forename: Choudhary M
authors.initials: CM
authors.lastname: Iqbal
authors.forename: Jacobus J Marion
authors.initials: JJ
authors.lastname: Meyer
authors.forename: Namrita
authors.initials: N
authors.lastname: Lall
authors.forename: Victor
authors.initials: V
authors.lastname: Kuete
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D001424:Bacterial Infections / Q000382:microbiology*; D029760:Garcinia / Q000737:chemistry*; D006801:Humans; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry*; D027821:Rutaceae / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts