title: Phylogenetic analysis of immunomodulatory protein genes of camelpoxvirus obtained from India.
abstract: The haemagglutinin (HA) encoding gene and genes encoding for immunomodulatory proteins i.e., schlafen-like protein, epidermal growth factor and golgi anti apoptotic protein of camelpoxvirus (CMLV) obtained from Indian dromedarian camels were cloned and characterized. In this study, the size of the HA encoding gene obtained from the Indian CMLV is 941 bp which is only partial. Sequence analysis of schlafen-like protein gene revealed that CMLV obtained from India shared 99.6% identity with CMLV-Iran and CMLV-Kazakhstan strains both at nucleotide and amino acid level. The size of epidermal growth factor (EGF) gene of Indian CMLV obtained in this study was 418 bp, which was due to the addition of one cytosine residue position 132 of EGF gene of Indian CMLV. Sequence analysis revealed that the Golgi anti-apoptotic protein (GAAP) of Indian CMLV shared 99.5% sequence identity both at the nucleotide and amino acid level with CMLV-Kazakhstan. Based on the nucleotide and amino acid sequence identities and phylogenetic analyses of these genes, it is found that CMLV-India is forming a cluster with Kazakhstan and Iranian CMLV isolates.
authors.affiliation: National Research Centre on Camel, Post Bag No. 7, Jorbeer, Bikaner 334 001, Rajasthan, India. camelnag@yahoo.com
authors.forename: G
authors.initials: G
authors.lastname: Nagarajan
authors.forename: Shelesh Kumar
authors.initials: SK
authors.lastname: Swami
authors.forename: Shyam Singh
authors.initials: SS
authors.lastname: Dahiya
authors.forename: G
authors.initials: G
authors.lastname: Sivakumar
authors.forename: Vinay Kumar
authors.initials: VK
authors.lastname: Yadav
authors.forename: F C
authors.initials: FC
authors.lastname: Tuteja
authors.forename: S D
authors.initials: SD
authors.lastname: Narnaware
authors.forename: N V
authors.initials: NV
authors.lastname: Patil
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002162:Camelus / Q000235:genetics / Q000276:immunology / Q000821:virology*; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D006388:Hemagglutinins / Q000235:genetics / Q000276:immunology*; D007155:Immunologic Factors / Q000235:genetics / Q000276:immunology*; D007194:India; D008969:Molecular Sequence Data; D018155:Orthopoxvirus / Q000235:genetics / Q000276:immunology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011213:Poxviridae Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D004279:DNA, Viral; D006388:Hemagglutinins; D007155:Immunologic Factors