title: Short-cut nitrification in biological aerated filters with modified zeolite and nitrifying sludge.
abstract: Autotrophic domestication for short-cut nitrification (SCN) and microorganism immobilization was carried out in a lab-scale biological aerated filters (BAFs) system with activated sludge. Zeolite was chosen as fillings and modified to enlarge the specific surface and to remove toxic metal ions. After thirty-day domestication and immobilization, the NH4(+)-N removal capacity increased to 76.51 mg/g dry sludge (DS) and the ratio of NO2(-)-N converted from NH4(+)-N reached to 91.2% finally. The analysis of growth kinetics indicated that free ammonia should be the key factor for SCN. The abundance variation of nitrifiers, measured by qPCR, showed that AOB was enriched successfully and NOB was washed out. The results also showed that modified zeolite should be more beneficial to the specific immobilization of AOB than natural zeolite. The shift in the community structure of AOB during the domestication by DGGE profile was investigated.
authors.affiliation: Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, Nankai University, 94 Weijin Road, Tianjin 300071, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Li
authors.forename: Shifang
authors.initials: S
authors.lastname: Sun
authors.forename: Tingfeng
authors.initials: T
authors.lastname: Guo
authors.forename: Chao
authors.initials: C
authors.lastname: Yang
authors.forename: Cunjiang
authors.initials: C
authors.lastname: Song
authors.forename: Weitao
authors.initials: W
authors.lastname: Geng
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Feng
authors.forename: Shufang
authors.initials: S
authors.lastname: Wang
mesh_terms: D064751:Ammonium Compounds / Q000378:metabolism; D052818:Autotrophic Processes / Q000187:drug effects; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D018441:Biofilms / Q000187:drug effects; D019149:Bioreactors / Q000382:microbiology*; D015169:Colony Count, Microbial; D058645:Denaturing Gradient Gel Electrophoresis; D005374:Filtration / Q000295:instrumentation*; D005798:Genes, Bacterial / Q000235:genetics; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D008969:Molecular Sequence Data; D058465:Nitrification / Q000187:drug effects*; D009584:Nitrogen / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology*; D017641:Zeolites / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D064751:Ammonium Compounds; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D017641:Zeolites; D009584:Nitrogen