title: Molecular assessment of minimal residual disease in PBSC harvests provides prognostic information in neuroblastoma.
abstract: As almost all patients with high-risk neuroblastomas have autograft, we aimed to determine if minimal residual disease (MRD) quantified by RT-PCR for tyrosine hydroxylase in PBSC is prognostic in neuroblastomas. PBSC harvests from 38 children were analyzed. Seven had harvests positive for TH-mRNA. Patients with a positive MRD had a lower 2-year-overall-survival compared to those with negative MRD (P = 0.04) regardless of whether or not PBSC were re-infused. Patients in CR/VGPR group with positive MRD have hazard ratio of death at 7.3 [1.3-40.5]. In conclusion, molecular MRD status in PBSC of good response group may be of interest as a survival prognostic factor in high-risk neuroblastomas.
authors.affiliation: CHU de Clermont-Ferrand, Centre Régional de Cancérologie et Thérapie Cellulaire Pédiatrique, Hôpital Estaing, Clermont-Ferrand, France.
authors.forename: F
authors.initials: F
authors.lastname: Chambon
authors.forename: A
authors.initials: A
authors.lastname: Tchirkov
authors.forename: B
authors.initials: B
authors.lastname: Pereira
authors.forename: E
authors.initials: E
authors.lastname: Rochette
authors.forename: F
authors.initials: F
authors.lastname: Deméocq
authors.forename: J
authors.initials: J
authors.lastname: Kanold
mesh_terms: D002648:Child; D002675:Child, Preschool; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism; D018365:Neoplasm, Residual; D009447:Neuroblastoma / Q000201:enzymology / Q000235:genetics / Q000401:mortality* / Q000628:therapy*; D036102:Peripheral Blood Stem Cell Transplantation; D011446:Prospective Studies; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D015996:Survival Rate; D014182:Transplantation, Autologous; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics / Q000378:metabolism
chemical_list: D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D014446:Tyrosine 3-Monooxygenase
keywords: RQ-PCR for tyrosine hydroxylase mRNA; minimal residual disease; neuroblastoma; peripheral blood stem cells