title: Efficient propagation of equine viruses in a newly established equine cell line, FHK-13.1 cells.
abstract: Equine cells are required for isolation of viruses that infect the horse. However, only a few equine cell lines and cell cultures are available so far. Fetal horse kidney (FHK)-Tcl3.1 cell is a novel cell line established by introducing simian virus 40 (SV40) large T antigen. In the present study, the ability to propagate equine viruses was compared between FHK-Tcl3.1 cells and other equine cells. FHK-Tcl3.1 cells efficiently increased many viruses derived from or having pathogenicity to horses and produced high infective titers in culture fluids. These results indicate that FHK-Tcl3.1 cells would be useful for propagation and serological tests of viruses that affect Equidae.
authors.affiliation: Laboratory of Veterinary Epizootiology, Department of Veterinary Medicine, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Oguma
authors.forename: Maiko
authors.initials: M
authors.lastname: Ishida
authors.forename: Ken
authors.initials: K
authors.lastname: Maeda
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sentsui
mesh_terms: D000256:Adenoviridae; D000818:Animals; D000957:Antigens, Viral, Tumor; D005537:Aphthovirus; D018929:Cell Culture Techniques / Q000662:veterinary; D002460:Cell Line / Q000166:cytology / Q000821:virology*; D003588:Cytopathogenic Effect, Viral; D005333:Fetus / Q000166:cytology*; D006736:Horses / Q000821:virology*; D007668:Kidney / Q000166:cytology*; D013539:Simian virus 40; D018140:Varicellovirus; D018116:Vesiculovirus
chemical_list: D000957:Antigens, Viral, Tumor