title: Nicotinamide nucleotide transhydrogenase mRNA expression is related to human obesity.
abstract: OBJECTIVE
  A spontaneous deletion in the nicotinamide nucleotide transhydrogenase (Nnt) gene eliminating exons 7-11 in C57BL/6J (B6J) mice is associated with reduced glucose-stimulated insulin secretion in vitro, impaired glucose tolerance, higher epigonadal fat mass, and altered susceptibility to diet induced obesity of male B6J mice was proposed. A potential implication for NNT in human adipose tissue distribution has not been investigated so far.
  
  
  METHODS
  Therefore, NNT mRNA expression in paired human samples of visceral (vis) and subcutaneous (sc) adipose tissue from 221 subjects with a wide range of body mass index (BMI), insulin sensitivity, and glucose tolerance was analyzed.
  
  
  RESULTS
  NNT mRNA expression is significantly higher in visceral fat of obese patients and correlates with body weight, BMI, % body fat, visceral and sc fat area, waist and hip circumference, and fasting plasma insulin (FPI). Multivariate linear regression analysis revealed visceral NNT expression as age and gender independent predictor of BMI, waist circumference, visceral fat area, and % body fat, but not FPI and 2 h OGTT glucose.
  
  
  CONCLUSIONS
  In conclusion, a functional relevance of NNT in the development of human obesity and visceral fat distribution was suggested here.
authors.affiliation: Department of Medicine, Endocrinology and Diabetes, Universität Leipzig, Leipzig, Germany.
authors.forename: John T
authors.initials: JT
authors.lastname: Heiker
authors.forename: Matthias
authors.initials: M
authors.lastname: Kern
authors.forename: Joanna
authors.initials: J
authors.lastname: Kosacka
authors.forename: Gesine
authors.initials: G
authors.lastname: Flehmig
authors.forename: Michael
authors.initials: M
authors.lastname: Stumvoll
authors.forename: Edward
authors.initials: E
authors.lastname: Shang
authors.forename: Tobias
authors.initials: T
authors.lastname: Lohmann
authors.forename: Miriam
authors.initials: M
authors.lastname: Dreßler
authors.forename: Peter
authors.initials: P
authors.lastname: Kovacs
authors.forename: Matthias
authors.initials: M
authors.lastname: Blüher
authors.forename: Nora
authors.initials: N
authors.lastname: Klöting
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001786:Blood Glucose / Q000032:analysis; D001823:Body Composition; D015992:Body Mass Index; D001835:Body Weight; D004032:Diet; D005215:Fasting; D005260:Female; D005786:Gene Expression Regulation; D005951:Glucose Tolerance Test; D006801:Humans; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D050152:Intra-Abdominal Fat / Q000378:metabolism*; D016014:Linear Models; D008297:Male; D008875:Middle Aged; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D015999:Multivariate Analysis; D061087:NADP Transhydrogenase, AB-Specific / Q000235:genetics / Q000378:metabolism*; D009765:Obesity / Q000235:genetics* / Q000503:physiopathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D050151:Subcutaneous Fat / Q000378:metabolism; D055815:Young Adult
chemical_list: D001786:Blood Glucose; D007328:Insulin; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; D061087:NADP Transhydrogenase, AB-Specific; C556045:NNT protein, human