title: One-pot three-enzyme synthesis of UDP-Glc, UDP-Gal, and their derivatives.
abstract: A UTP-glucose-1-phosphate uridylyltransferase (SpGalU) and a galactokinase (SpGalK) were cloned from Streptococcus pneumoniae TIGR4 and were successfully used to synthesize UDP-galactose (UDP-Gal), UDP-glucose (UDP-Glc), and their derivatives in an efficient one-pot reaction system. The reaction conditions for the one-pot multi-enzyme synthesis were optimized and nine UDP-Glc/Gal derivatives were synthesized. Using this system, six unnatural UDP-Gal derivatives, including UDP-2-deoxy-Galactose and UDP-GalN3 which were not accepted by other approach, can be synthesized efficiently in a one pot fashion. More interestingly, this is the first time it has been reported that UDP-Glc can be synthesized in a simpler one-pot three-enzyme synthesis reaction system.
authors.affiliation: The State Key Laboratory of Microbial Technology and National Glycoengineering Research Center, Shandong University, Jinan, Shandong 250100, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zou
authors.forename: Mengyang
authors.initials: M
authors.lastname: Xue
authors.forename: Wenjun
authors.initials: W
authors.lastname: Wang
authors.forename: Li
authors.initials: L
authors.lastname: Cai
authors.forename: Leilei
authors.initials: L
authors.lastname: Chen
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Peng George
authors.initials: PG
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Shen
authors.forename: Min
authors.initials: M
authors.lastname: Chen
mesh_terms: D060326:Chemistry Techniques, Synthetic; D003001:Cloning, Molecular; D005686:Galactokinase / Q000235:genetics / Q000378:metabolism*; D013296:Streptococcus pneumoniae / Q000201:enzymology / Q000235:genetics; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase / Q000235:genetics / Q000378:metabolism*; D014531:Uridine Diphosphate Galactose / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism; D014532:Uridine Diphosphate Glucose / Q000737:chemistry*
chemical_list: C082837:UDP-2-deoxygalactose; D014531:Uridine Diphosphate Galactose; D005686:Galactokinase; D005957:UTP-Glucose-1-Phosphate Uridylyltransferase; D014532:Uridine Diphosphate Glucose