title: Antibody responses induced in mice immunized with recombinant adenovectors expressing chimeric proteins of various porcine pathogens.
abstract: Porcine reproductive and respiratory syndrome virus (PRRSV), Porcine Circovirus type 2 (PCV2) and Mycoplasma (M.) hyopneumoniae are swine pathogens of economic importance. Although vaccines are available for each pathogen, no commercial bivalent vaccines have been developed so far. In this study, recombinant adenovectors (AdVs) expressing proteins of each pathogen were developed and their immunogenicity tested in mice. The proteins of interest were the glycoprotein GP5 of PRRSV, the capsid (Cap) protein of PCV2b and the C-terminal portion of P97 (P97c) protein of M. hyopneumoniae that were used alone and/or in fusion which each other. Inoculation of mice with the AdVs resulted in antibody (Ab) responses specific to the immunogens. Unexpectedly, immunization with vaccines expressing P97c in fusion to either Cap or GP5 enhanced the Ab responses against Cap and GP5, suggesting an immunopotentiator effect for P97c.
authors.affiliation: University of Québec at Montréal, Department of Biological Sciences, P.O. Box 8888, Succursale Centre-Ville, Montréal, Québec H3C 3P8, Canada.
authors.forename: Elodie
authors.initials: E
authors.lastname: Roques
authors.forename: Aurélie
authors.initials: A
authors.lastname: Girard
authors.forename: Carl A
authors.initials: CA
authors.lastname: Gagnon
authors.forename: Denis
authors.initials: D
authors.lastname: Archambault
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D018829:Adhesins, Bacterial / Q000276:immunology; D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000276:immunology; D000917:Antibody Formation; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology*; D036022:Capsid Proteins / Q000276:immunology; D005260:Female; D051379:Mice; D008807:Mice, Inbred BALB C; D045705:Mycoplasma hyopneumoniae / Q000276:immunology*; D045729:Pneumonia of Swine, Mycoplasmal / Q000276:immunology* / Q000517:prevention & control; D019318:Porcine Reproductive and Respiratory Syndrome / Q000276:immunology* / Q000517:prevention & control; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology*; D013552:Swine; D014611:Vaccination / Q000662:veterinary*; D017778:Vaccines, Combined / Q000008:administration & dosage / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D018829:Adhesins, Bacterial; D000914:Antibodies, Viral; D001428:Bacterial Vaccines; D036022:Capsid Proteins; C092236:P97 protein, Mycoplasma; D017778:Vaccines, Combined; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C114789:glycoprotein 5, PRRSV