title: Development of an anti-norovirus single-chain Fv for immunochromatographic test kit.
abstract: It was developed that an anti-norovirus single-chain Fv (scFv) antibody for immunochromathographic test kit by using the phage displayed technique and biopanning. To obtain the scFv having wide reactivity for several norovirus genotypes, a mixture of recombinant norovirus capsid proteins, including 11 norovirus genotypes, was used for biopanning. Then, one anti-norovirus scFv antibody that recognized both of norovirus genogroups GI and GII was identified. An immunochromatographic test strip was constructed by using the scFv and demonstrated it to detect norovirus in stool samples. The immunochromatographic strip showed similar sensitivity to a commercially available one on which several monoclonal antibodies are included.
authors.affiliation: Faculty of Information Science and Electrical Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan. nakano@ees.kyushu-u.ac.jp
authors.forename: Michihiko
authors.initials: M
authors.lastname: Nakano
authors.forename: Keita
authors.initials: K
authors.lastname: Murofushi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Watabe
authors.forename: Toshiya
authors.initials: T
authors.lastname: Ohta
mesh_terms: D002846:Chromatography, Affinity / Q000295:instrumentation*; D006801:Humans; D029322:Norovirus / Q000276:immunology*; D057127:Single-Chain Antibodies / Q000276:immunology*
chemical_list: D057127:Single-Chain Antibodies