title: Monitoring of protease catalyzed reactions by quantitative MALDI MS using metal labeling.
abstract: Quantitative mass spectrometry is a powerful tool for the determination of enzyme activities as it does not require labeled substrates and simultaneously allows for the identification of reaction products. However, major restrictions are the limited number of samples which can be measured in parallel due to the need for isotope labeled internal standards. Here we describe the use of metal labeling of peptides for the setup of multiplexed enzyme activity assays. After proteolytic reaction, using the protease trypsin, remaining substrates and peptide products formed in the reaction were labeled with metal chelators complexing rare earth metal ions. Labeled peptides were quantified with high accuracy and over a wide dynamic range (at least 2 orders of magnitude) using MALDI MS in case of simple peptide mixtures or by LC-MALDI MS for complex substrate mixtures and used for the monitoring of time-dependent product formation and substrate consumption. Due to multiplexing capabilities and accuracy, the presented approach will be useful for the determination of enzyme activities with a wide range of biochemical and biotechnological applications.
authors.affiliation: Institute for Experimental Medicine-Division Systematic Proteome Research, Christian-Albrechts-Universität, Kiel, Germany.
authors.forename: Barbara
authors.initials: B
authors.lastname: Gregorius
authors.forename: Thomas
authors.initials: T
authors.lastname: Jakoby
authors.forename: Dirk
authors.initials: D
authors.lastname: Schaumlöffel
authors.forename: Andreas
authors.initials: A
authors.lastname: Tholey
mesh_terms: D000595:Amino Acid Sequence; D055162:Biocatalysis; D057075:Enzyme Assays / Q000379:methods*; D007700:Kinetics; D008670:Metals / Q000737:chemistry*; D009496:Neurotensin / Q000737:chemistry / Q000378:metabolism; D059748:Proteolysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013194:Staining and Labeling; D014357:Trypsin / Q000378:metabolism*
chemical_list: D008670:Metals; D009496:Neurotensin; D014357:Trypsin