title: WKYMVm-induced cross-talk between FPR2 and HGF receptor in human prostate epithelial cell line PNT1A.
abstract: Cross-communication between GPCRs and TKRs represents a mechanism to amplify the information exchange throughout the cell. We show that WKYMVm, an FPR2 agonist, induces the phosphorylation of Y1313/Y1349/Y1356 residues of c-Met and triggers some of the molecular responses elicited by c-Met/HGF binding, such as STAT3, PLC-γ1/PKCα and PI3K/Akt pathways. The critical role of NADPH oxidase-dependent superoxide generation in this cross-talk mechanism is supported by the finding that blockade of NADPH oxidase function prevents c-Met trans-phosphorylation and the downstream signalling cascade. These results highlight the function of FPR2 to activate a interconnected signalling network and suggest novel possibilities for therapeutic interventions.
authors.affiliation: Department of Biochemistry and Medical Biotechnology, University of Naples Federico II, Via S. Pansini 5, 80131 Naples, Italy.
authors.forename: Fabio
authors.initials: F
authors.lastname: Cattaneo
authors.forename: Melania
authors.initials: M
authors.lastname: Parisi
authors.forename: Rosario
authors.initials: R
authors.lastname: Ammendola
mesh_terms: D020134:Catalytic Domain / Q000235:genetics; D002460:Cell Line; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008297:Male; D009842:Oligopeptides / Q000494:pharmacology*; D010766:Phosphorylation / Q000187:drug effects; D011467:Prostate / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D019859:Proto-Oncogene Proteins c-met / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000494:pharmacology; D020239:Receptor Cross-Talk / Q000187:drug effects*; D044042:Receptors, Formyl Peptide / Q000819:agonists / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D044067:Receptors, Lipoxin / Q000819:agonists / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D014443:Tyrosine / Q000235:genetics / Q000378:metabolism
chemical_list: C105101:FPR2 protein, human; D009842:Oligopeptides; D034741:RNA, Small Interfering; D044042:Receptors, Formyl Peptide; D044067:Receptors, Lipoxin; C113617:Trp-Lys-Tyr-Met-Val-Met; D014443:Tyrosine; D019859:Proto-Oncogene Proteins c-met