title: [Quantitative models for Baicalin content using NIR technology for the study of Shang Jie plaster].
abstract: A dynamic prediction model for the content of Baicalin in Shang Jie plasters extract solutions was developed using near-infrared spectroscopy in transmission mode. Sixty five spectra were obtained through near-infrared transmission mode during extracting process. Refering to the content of Baicalin performed by reversed-phase high performance liquid chromatography (HPLC), the calibration model was developed with the application of partial least squares regression algorithm (PLSR). The constructed model was validated by 30 samples; some parameters of the calibration model were optimized by cross-validation. The root mean square error (RMSECV) of Baicalin was 0.006 8 mg x g(-1), the correlation coefficient (R) was 0.9991, and the optimal dimension factor was 8; After predicted by test set, the root mean square error (RMSEP) and correlation coefficient (R) of prediction obtained were 0.009 2 mg x g(-1) and 0.998 7 respectively. This work demonstrated that NIR spectroscopy combined with PLS could be used for the determination of Baicalin in Shang Jie plasters extract.
authors.affiliation: School of Pharmaceutical Science and Technology, Dalian State Key Laboratory of Fine Chemical Engineering, Dalian University of Technology, Dalian 116023, China. jbhjby198571@163.com
authors.forename: Bo-Hai
authors.initials: BH
authors.lastname: Jiang
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.forename: Shi-Sheng
authors.initials: SS
authors.lastname: Wang
authors.forename: Rui
authors.initials: R
authors.lastname: Cai
authors.forename: Wei-Jie
authors.initials: WJ
authors.lastname: Zhao
mesh_terms: D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis*; D016018:Least-Squares Analysis; D008962:Models, Theoretical; D011786:Quality Control; D019265:Spectroscopy, Near-Infrared / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; C038044:baicalin