title: Differences between 4-fluoroaniline degradation and autoinducer release by Acinetobacter sp. TW: implications for operating conditions in bacterial bioaugmentation.
abstract: To develop a bacterial bioaugmentation system for fluorine-containing industrial wastewater treatment, optimal conditions for 4-fluoroaniline (4-FA) degradation and autoinducer release in Acinetobacter sp. TW were determined. Quorum sensing in biofilms of strain TW was also investigated. Different optimal conditions exist for 4-FA degradation and autoinducer release, particularly with regard to pH. Quorum sensing modulates extracellular polymeric substance (EPS) secretion and biofilm formation in the strain but plays no role in 4-FA degradation. Under optimal conditions for 4-FA degradation, the release of N-3-oxo-hexanoyl-homoserine lactone (3-oxo-C6-HSL) and N-hexanoyl-homoserine lactone (C6-HSL) in strain TW was significantly lower than required for quorum sensing. Under optimal conditions for autoinducer release, on the other hand, 3-oxo-C6-HSL and C6-HSL levels exceeded the quorum sensing thresholds, thereby inducing EPS secretion and biofilm formation. We conclude that the optimal conditions for autoinducer release (25 °C, pH 5, 800 mg L(-1) 4-FA, and 0 % NaCl) are suitable for bacterial colonization in bioaugmentation, while those for 4-FA degradation (25-30 °C, pH 8 and 800 mg L(-1) 4-FA) maximize the system performance after colonization.
authors.affiliation: College of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou, 310012, China. wmzyy@163.com
authors.forename: Meizhen
authors.initials: M
authors.lastname: Wang
authors.forename: Junjie
authors.initials: J
authors.lastname: Xu
authors.forename: Juehua
authors.initials: J
authors.lastname: Wang
authors.forename: Shuo
authors.initials: S
authors.lastname: Wang
authors.forename: Huajun
authors.initials: H
authors.lastname: Feng
authors.forename: Jiali
authors.initials: J
authors.lastname: Shentu
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Shen
mesh_terms: D000150:Acinetobacter / Q000235:genetics / Q000378:metabolism*; D000814:Aniline Compounds / Q000737:chemistry*; D001673:Biodegradation, Environmental; D018441:Biofilms; D015964:Gene Expression Regulation, Bacterial; D009068:Movement; D010675:Pheromones / Q000737:chemistry / Q000378:metabolism*; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D000814:Aniline Compounds; D010675:Pheromones; C043455:4-fluoroaniline; D001616:beta-Galactosidase