title: Glyconanoparticle aided detection of β-amyloid by magnetic resonance imaging and attenuation of β-amyloid induced cytotoxicity.
abstract: The development of a noninvasive method for the detection of Alzheimer's disease is of high current interest, which can be critical in early diagnosis and in guiding treatment of the disease. The aggregates of β-amyloid are a pathological hallmark of Alzheimer's disease. Carbohydrates such as gangliosides have been shown to play significant roles in initiation of amyloid aggregation. Herein, we report a biomimetic approach using superparamagnetic iron oxide glyconanoparticles to detect β-amyloid. The bindings of β-amyloid by the glyconanoparticles were demonstrated through several techniques including enzyme linked immunosorbent assay, gel electrophoresis, tyrosine fluorescence assay, and transmission electron microscopy. The superparamagnetic nature of the nanoparticles allowed easy detection of β-amyloid both in vitro and ex vivo by magnetic resonance imaging. Furthermore, the glyconanoparticles not only were nontoxic to SH-SY5Y neuroblastoma cells but also greatly reduced β-amyloid induced cytotoxicity to cells, highlighting the potential of these nanoparticles for detection and imaging of β-amyloid.
authors.affiliation: Department of Chemistry, 578 S. Shaw Lane, Room 426, Michigan State University, East Lansing, MI 48824, USA.
authors.forename: Hovig
authors.initials: H
authors.lastname: Kouyoumdjian
authors.forename: David C
authors.initials: DC
authors.lastname: Zhu
authors.forename: Mohammad H
authors.initials: MH
authors.lastname: El-Dakdouki
authors.forename: Kelly
authors.initials: K
authors.lastname: Lorenz
authors.forename: Jianjun
authors.initials: J
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Xuefei
authors.initials: X
authors.lastname: Huang
mesh_terms: D016229:Amyloid beta-Peptides / Q000633:toxicity*; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D016537:Gangliosidosis, GM1; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D019158:N-Acetylneuraminic Acid / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*
chemical_list: D016229:Amyloid beta-Peptides; D019158:N-Acetylneuraminic Acid