title: Adipogenic effects of piperlonguminine in 3T3-L1 cells and plasma concentrations of several amide constituents from Piper chaba extracts after treatment of mice.
abstract: In our previous study, piperlonguminine from the fruit of Piper chaba was reported to promote adipogenesis in 3T3-L1 cells like the peroxisome proliferator-activated receptor-γ (PPARγ) agonist, troglitazone. In the present study, the mode of action of piperlonguminine in cells was examined. Piperlonguminine increased mRNA levels of adiponectin, glucose transporter 4, and fatty acid-binding protein (aP2). It also increased mRNA levels of PPARγ2 but, unlike troglitazone, piperlonguminine did not activate PPARγ directly in a nuclear receptor cofactor assay. Analyses of plasma from mice treated with piperlonguminine, piperine, and retrofractamide A, and an extract of the fruit, showed that concentrations of piperlonguminine were higher than those of piperine and retrofractamide A, and that the "area-under-the-curve" of piperine increased following in vivo administration of the extract.
authors.affiliation: Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Kyoto, 607-8412, Japan.
authors.forename: Itadaki
authors.initials: I
authors.lastname: Yamaguchi
authors.forename: Hisashi
authors.initials: H
authors.lastname: Matsuda
authors.forename: Hailong
authors.initials: H
authors.lastname: Zhang
authors.forename: Makoto
authors.initials: M
authors.lastname: Hamao
authors.forename: Chihiro
authors.initials: C
authors.lastname: Yamashita
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Kogami
authors.forename: Haruka
authors.initials: H
authors.lastname: Kon'I
authors.forename: Megumi
authors.initials: M
authors.lastname: Murata
authors.forename: Seikou
authors.initials: S
authors.lastname: Nakamura
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yoshikawa
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects*; D052242:Adiponectin / Q000235:genetics / Q000378:metabolism; D000470:Alkaloids / Q000494:pharmacology; D000577:Amides / Q000097:blood / Q000302:isolation & purification / Q000493:pharmacokinetics*; D000818:Animals; D019540:Area Under Curve; D052117:Benzodioxoles / Q000097:blood / Q000302:isolation & purification / Q000493:pharmacokinetics* / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D003847:Deoxyglucose / Q000378:metabolism; D004148:Dioxolanes / Q000097:blood / Q000302:isolation & purification / Q000493:pharmacokinetics*; D004305:Dose-Response Relationship, Drug; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000378:metabolism; D005638:Fruit / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D047495:PPAR gamma / Q000819:agonists / Q000235:genetics / Q000378:metabolism; D008517:Phytotherapy; D031701:Piper / Q000737:chemistry; D010880:Piperidines / Q000494:pharmacology; D010936:Plant Extracts / Q000097:blood / Q000302:isolation & purification / Q000493:pharmacokinetics*; D010946:Plants, Medicinal; D053284:Polyunsaturated Alkamides / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism; D010881:Piperidones
chemical_list: D052242:Adiponectin; D000470:Alkaloids; D000577:Amides; D052117:Benzodioxoles; D004268:DNA-Binding Proteins; D003847:Deoxyglucose; D004148:Dioxolanes; D050556:Fatty Acid-Binding Proteins; D047495:PPAR gamma; D010880:Piperidines; D010936:Plant Extracts; D053284:Polyunsaturated Alkamides; D012333:RNA, Messenger; D014157:Transcription Factors; D014280:Triglycerides; C499919:Adipoq protein, mouse; C528734:GLUT4 enhancer factor, mouse; C568084:refrofractamide A; C498077:piperlongumine; D010881:Piperidones; C008922:piperine