title: Biomedical studies on lipid peroxidation and erythrocyte fragility during the process of aging.
abstract: OBJECTIVE
  To investigate oxidative stress, hemoglobin percentage and erythrocyte osmotic fragility in various aging groups.
  
  
  METHODS
  A total of 200 healthy volunteers of both genders between age group 20-65 years were selected by random method. Determination of hemoglobin percentage was done employing modified cyanide method of Dacie and Lewis. The erythrocyte lysis was observed in hypotonic solution of buffered saline at varying concentrations and optical density was measured at 540 nm. The extent of lipid peroxidation in form of malondialdehyde was measured by thiobarbituric acid method.
  
  
  RESULTS
  The study found a significant decrease in hemoglobin percentage, increase in erythrocyte osmotic fragility and increased lipid peroxidation in form of malondialdehyde with increasing age.
  
  
  CONCLUSIONS
  Supplementation of antioxidants may prevent the oxidative injury in elderly group of subjects.
authors.affiliation: Department of Biochemistry, International Medical School Management and Science University, MSU Holdings Sdn. Bhd.University Drive, Off Persiaran Olahraga, Section 13, 40100 Shah Alam, Selangor Darul Ehsan, Malaysia.
authors.forename: Arun
authors.initials: A
authors.lastname: Kumar
mesh_terms: D000328:Adult; D000368:Aged; D000375:Aging / Q000502:physiology*; D004912:Erythrocytes / Q000737:chemistry*; D005260:Female; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D015227:Lipid Peroxidation / Q000502:physiology*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis / Q000378:metabolism; D008875:Middle Aged; D009996:Osmotic Fragility; D018384:Oxidative Stress; D055815:Young Adult
chemical_list: D006454:Hemoglobins; D008315:Malondialdehyde
keywords: Aging; Erythrocyte osmotic fragility; Hemoglobin; Lipid peroxidation