title: Impact of fungal load on diagnosis and outcome of allergic fungal rhinosinusitis.
abstract: Fungal load colonization may modify the classic eosinophilic inflammation in allergic fungal rhinosinusitis (AFRS). We aimed to evaluate the impact of fungal load on diagnosis and outcome of AFRS. In the present cohort study fungal load differences were determined prospectively according to Gomori methenamine silver (GMS) fungal stained (histopathological and cytological examination) with the tenacious mucus, cheesy clay-like materials and sinus mucosa/polyps in 12 AFRS patients. Two groups with different fungal loads, AFRS with (six patients) and without (six patients) high fungal loads (HFL) were evaluated for nasal endoscopic score, paranasal sinuses CT score, histopathological and immunohistochemical changes. Endoscopic outcome scoring differences were evaluated for 1 year after endoscopic sinus surgery and 1 month oral corticosteroids treatment. No differences were observed between both groups (AFRS with/without HFL) concerning the total CT score and opacification features (P > 0.05). Eosinophils and CD3 + CD8 + T cell were dominant in both groups. More edema and less fibrosis were observed in HFL group. Gliotoxin producers Aspergilli were present in all HFL in comparison to 5/6 (83.3%) in cases without HFL. Fewer patients 1/6 (16.6%) and less number of recurrences/year 0.1 ± 0.4 occurred in the AFRS with HFL compared to the AFRS without HFL [5/6 (83.3%) and 1.16 ± 0.7) (P = 0.021 and 0.023, respectively]. In addition to mucus and mucosal tissues, cheesy clay-like materials must be assessed in AFRS cases. Although patients of AFRS with HFL had negligible clinical differences from ordinary AFRS without HFL, they had better outcome after treatment.
authors.affiliation: Otorhinolaryngology Department, ORL H&N Surgery, Menoufia University Hospital, Shebin El-Kom, Egypt, ahmedragab2000@hotmail.com.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Ragab
authors.forename: Rehab Monir
authors.initials: RM
authors.lastname: Samaka
authors.forename: Mohamed
authors.initials: M
authors.lastname: Salem
mesh_terms: D000328:Adult; D015331:Cohort Studies; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009298:Nasal Polyps / Q000175:diagnosis / Q000382:microbiology / Q000601:surgery; D011446:Prospective Studies; D012008:Recurrence; D012221:Rhinitis, Allergic, Perennial / Q000175:diagnosis* / Q000382:microbiology* / Q000473:pathology / Q000601:surgery; D012852:Sinusitis / Q000175:diagnosis* / Q000382:microbiology* / Q000473:pathology / Q000601:surgery; D013101:Sphenoid Sinus / Q000000981:diagnostic imaging; D014057:Tomography, X-Ray Computed; D016896:Treatment Outcome; D055815:Young Adult