title: Effects of decontamination at varying contamination levels of Campylobacter jejuni on broiler meat.
abstract: When assessing effects of decontamination techniques on counts of Campylobacter spp. on broiler meat, it is essential that the results reflect the variations that may exist. Decontamination studies often use high inoculation levels (10(7) to 10(8) cfu) and one or few strains of Campylobacter jejuni, thereby restricting the results to reflect only a limited part of the true situation. This study presents results from physical and chemical decontamination of broiler meat medallions using different strains and different initial concentrations of C. jejuni. For 3 strains of C. jejuni, mean log reductions obtained by freezing at -20°C for 7 d was significantly higher for an initial concentration of 10(7) cfu/sample on the meat compared with an initial concentration of 10(3) cfu/sample. For freezing at -20°C for 24 h or application of 6% tartaric acid and subsequent storage for 24 h, no statistically significant difference in reductions was found for initial concentrations ranging from 10(3) to 10(7) cfu per sample. The mean log reductions obtained by all techniques were strongly dependent on the strain tested. The results reveal that reductions obtained with high inoculation levels of C. jejuni (10(7) cfu/sample) or single or few strains of the species (or both) should not be interpreted as a generic result for the species. If inoculation studies cannot be replaced by investigations of naturally contaminated meat, we advise using a mixture of strains found in the production environment at levels as close as possible to the natural contamination level.
authors.affiliation: National Food Institute, Division of Epidemiology and Microbial Genomics, Technical University of Denmark, Moerkhoej Bygade 19, DK-2860 Soeborg, Denmark.
authors.forename: L
authors.initials: L
authors.lastname: Boysen
authors.forename: N S
authors.initials: NS
authors.lastname: Wechter
authors.forename: H
authors.initials: H
authors.lastname: Rosenquist
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D016123:Campylobacter jejuni / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification*; D002645:Chickens; D015169:Colony Count, Microbial; D003666:Decontamination / Q000379:methods*; D005615:Freezing; D008460:Meat / Q000382:microbiology*; D008462:Meat-Packing Industry / Q000379:methods*; D010710:Phosphates / Q000494:pharmacology; D011156:Population Density; D013644:Tartrates / Q000494:pharmacology; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D010710:Phosphates; D013644:Tartrates; C018279:sodium phosphate; C029768:tartaric acid