title: Kv7/M-type potassium channels in rat skin keratinocytes.
abstract: Skin keratinocytes fulfil important signalling and protective functions. Immunocytochemical experiments revealed the unexpected presence of immunoreactivity for the M-type potassium channel subunit Kv7.2 in the keratinocyte layer of intact rat paw skin and in keratinocytes isolated from the skin of 1-day-old rats and cultured in vitro for 3-10 days. Application of the M-channel enhancer retigabine (3-10 μM) to isolated cultured rat keratinocytes: (a) increased outward membrane currents recorded under voltage clamp, (b) produced ~3 mV hyperpolarization at rest, (c) enhanced ~3-fold the release of ATP induced by the TRPV3 agonist carvacrol (1 mM) and (d) increased the amplitude of the carvacrol-induced intracellular Ca(2+) transient measured with Fura-2. The effect of retigabine on ATP release was prevented by the M-channel blocking agent XE991. We conclude that rat skin keratinocytes possess M-channels that, when activated, can modify their physiological properties, with potential significance for their sensory and other biological functions.
authors.affiliation: Department of Neuroscience, Physiology and Pharmacology, University College London, Gower Street, London, WC1E 6BT, UK.
authors.forename: Joanne M
authors.initials: JM
authors.lastname: Reilly
authors.forename: Vsevolod
authors.initials: V
authors.lastname: Telezhkin
authors.forename: Gayle M
authors.initials: GM
authors.lastname: Passmore
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Marsh
authors.forename: David A
authors.initials: DA
authors.lastname: Brown
mesh_terms: D000200:Action Potentials; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D000873:Anthracenes / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D002219:Carbamates / Q000494:pharmacology; D002478:Cells, Cultured; D000081007:Cymenes; D051658:KCNQ2 Potassium Channel / Q000037:antagonists & inhibitors / Q000378:metabolism*; D015603:Keratinocytes / Q000378:metabolism* / Q000502:physiology; D039821:Monoterpenes / Q000494:pharmacology; D010655:Phenylenediamines / Q000494:pharmacology; D026902:Potassium Channel Blockers / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000166:cytology / Q000378:metabolism*; D050916:TRPV Cation Channels / Q000819:agonists / Q000378:metabolism
chemical_list: C112297:10,10-bis(4-pyridinylmethyl)-9(10H)-anthracenone; D000873:Anthracenes; D002219:Carbamates; D000081007:Cymenes; D051658:KCNQ2 Potassium Channel; D039821:Monoterpenes; D010655:Phenylenediamines; D026902:Potassium Channel Blockers; D050916:TRPV Cation Channels; C505767:TRPV3 protein, rat; C101866:ezogabine; D000255:Adenosine Triphosphate; C073316:carvacrol; D002118:Calcium