title: Comparative evaluation of cytokines in gingival crevicular fluid and saliva of patients with aggressive periodontitis.
abstract: OBJECTIVE
  This study aims to evaluate and compare cytokines in gingival crevicular fluid (GCF) and saliva of patients with aggressive periodontitis (AP) before and after treatment.
  
  
  METHODS
  Forty AP patients and 40 healthy volunteers were enrolled in this study. Clinical parameters included probing depth and sulcus bleeding index. GCF and saliva were collected from both groups. The levels of IL-1β, IL-2, IL-4, IL-6, IFN-γ and TNF-α were measured using ELISA.
  
  
  RESULTS
  The probing depth in AP patients was significantly deeper before treatment than after treatment. The concentrations of cytokines in GCF and saliva were significantly higher in AP patients than in the control group and decreased after periodontal treatment. Positive relationships were found between cytokine levels in GCF and clinical parameters. The reliability of cytokines in GCF and saliva was assessed by Cronbach's alpha analysis, which could be considered satisfactory.
  
  
  CONCLUSIONS
  Cytokine levels in GCF and saliva correlated well with clinical parameters and AP. Measurements of cytokines in saliva may be regarded as a noninvasive and quick method for monitoring periodontal disease activity.
authors.affiliation: State Key Laboratory for Oral Diseases and Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Sichuan - PR China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yue
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.forename: Chunjiao
authors.initials: C
authors.lastname: Xu
authors.forename: Wings T Y
authors.initials: WT
authors.lastname: Loo
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Guo
authors.initials: G
authors.lastname: Wen
authors.forename: Mary N B
authors.initials: MN
authors.lastname: Cheung
authors.forename: Lan-jun
authors.initials: LJ
authors.lastname: Bai
authors.forename: Yi-ding
authors.initials: YD
authors.lastname: Dou
authors.forename: Louis W C
authors.initials: LW
authors.lastname: Chow
authors.forename: Liang
authors.initials: L
authors.lastname: Hao
authors.forename: Ye
authors.initials: Y
authors.lastname: Tian
authors.forename: Jin-le
authors.initials: JL
authors.lastname: Li
authors.forename: Adrian Y S
authors.initials: AY
authors.lastname: Yip
authors.forename: Elizabeth L Y
authors.initials: EL
authors.lastname: Ng
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D055113:Chronic Periodontitis / Q000378:metabolism* / Q000628:therapy; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D005883:Gingival Crevicular Fluid / Q000378:metabolism*; D006801:Humans; D008297:Male; D012463:Saliva / Q000378:metabolism*; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: D015415:Biomarkers; D016207:Cytokines