title: Polymorphisms of survivin and its protein expression are associated with colorectal cancer susceptibility in Chinese population.
abstract: To investigate the association of survivin -31G/C, -141G/C, and -241T/C polymorphisms with colorectal cancer (CRC) susceptibility and explore the mechanisms of the survivin polymorphism in CRC development. A case-control study was conducted of 275 CRC cases and 270 healthy controls. Polymorphisms of survivin -31G/C, -141G/C, and -241T/C were genotyped by polymerase chain reaction-restriction fragment length polymorphism. Survivin and Ki-67 expression was analyzed by immunohistochemistry by the Envision technique for the paraffin sections of 152 CRC. It showed that the -31G/C genotype and allele distribution were significantly different between the CRC cases and controls. The -31CC genotype and -31C allele were over-represented among the CRC cases. Compared with the CC genotype, the GC and GG genotypes had a significantly decreased risk of CRC (p=0.015). Survivin and Ki-67 expression of patients with the CC genotype was significantly higher than the patients with the GC and GG genotypes. In addition, a significantly positive correlation was found between expression of Survivin and Ki-67. There were no significant difference of the -141G/C and -241T/C polymorphism distributions among cases and controls. Survivin 31G/C may adjust the Survivin expression, and it might contribute to a risk of developing CRC.
authors.affiliation: Department of Pathology, Luzhou Medical College, Luzhou, Sichuan, People's Republic of China.
authors.forename: Xia-Bin
authors.initials: XB
authors.lastname: Li
authors.forename: Shi-Ning
authors.initials: SN
authors.lastname: Li
authors.forename: Zhi-Hui
authors.initials: ZH
authors.lastname: Yang
authors.forename: Ling
authors.initials: L
authors.lastname: Cao
authors.forename: Fang-Lei
authors.initials: FL
authors.lastname: Duan
authors.forename: Xing-Wang
authors.initials: XW
authors.lastname: Sun
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People / Q000235:genetics / Q000706:statistics & numerical data; D002277:Carcinoma / Q000453:epidemiology / Q000208:ethnology / Q000235:genetics* / Q000378:metabolism; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D015179:Colorectal Neoplasms / Q000453:epidemiology / Q000208:ethnology / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics* / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length / Q000502:physiology; D020641:Polymorphism, Single Nucleotide / Q000502:physiology; D011153:Population; D000077022:Survivin
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D000077022:Survivin