title: Changed lineage composition is an early event in breast carcinogenesis.
abstract: The epithelium compartment of the human breast is made up of a branching ductal-lobular system, which is lined by a single layer of luminal epithelial cells surrounded by contractile myoepithelial cells. The co-ordinated development of these two cell types, and maintenance of their relative proportions, is fundamentally important for normal breast morphogenesis. Changes in cell type composition is one of the hallmark features of breast cancer progression, and the vast majority of breast tumors are comprised of luminal cells only, with a complete absence of myoepithelial cells. Despite this striking alteration in relative proportions of luminal and myoepithelial cells in invasive breast cancers compared with normal breast tissue, the steps in this dramatic change in cellular composition remain poorly characterised, nor is it known whether loss of myoepithelial cells is an early event in carcinogenesis. In a panel of breast tissues, we quantitated the proportion of luminal cells relative to the surrounding myoepithelial cell layer in a panel of normal and pre-invasive breast tissue samples, including lesions with proliferative disease without atypia (PDWA), columnar cell lesions (CCL), atypical ductal hyperplasia (ADH), and DCIS, and correlated these findings with proliferation in the same lesions. The study findings showed that changes in lineage composition correlate with increased proliferation, and are one of the earliest events in breast carcinogenesis. Therefore not only are myoepithelial cells important in distinguishing between invasive and non-invasive tumors, their relative proportion compared with luminal cell numbers may provide a new potential indicator of which premalignant lesions are at higher risk of progression to invasive disease.
authors.affiliation: Westmead Institute for Cancer Research, Sydney Medical School - Westmead, University of Sydney at Westmead Millennium Institute, Westmead, New South Wales, Australia. heidi.hilton@sydney.edu.au
authors.forename: Heidi N
authors.initials: HN
authors.lastname: Hilton
authors.forename: Silke
authors.initials: S
authors.lastname: Kantimm
authors.forename: J Dinny
authors.initials: JD
authors.lastname: Graham
authors.forename: Christine L
authors.initials: CL
authors.lastname: Clarke
mesh_terms: D001943:Breast Neoplasms / Q000473:pathology*; D063646:Carcinogenesis; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000473:pathology; D019070:Cell Lineage; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic; D004848:Epithelium / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D006965:Hyperplasia / Q000378:metabolism; D008856:Microscopy, Fluorescence; D011379:Prognosis; D012307:Risk Factors