title: Evaluation of erythroblast macrophage protein related to erythroblastic islands in patients with hematopoietic stem cell transplantation.
abstract: BACKGROUND
  Hematopoietic evaluation of the patients after Hematopoietic stem cell transplantation (HSCT) is very important. Erythroblast macrophage protein (Emp) is a key protein with function in normal differentiation of erythroid cells and macrophages. Emp expression correlates with erythroblastic island formation, a process widely believed to be associated with hematopoiesis in bone marrow. We aimed to investigate the hematopoietic function of bone marrow from 46 HSCT patients and 16 inpatients with severe anemia applied to the treatment of EPO by measuring Emp expression level.
  
  
  METHODS
  Emp mRNA and protein expression levels in mononuclear cells of bone marrow and peripheral blood samples were detected by RT-PCR and Western blotting method respectively.
  
  
  RESULTS
  While hematopoiesis occurs in bone marrow, Emp expression level was elevated and more erythroblastic islands were found , and Emp is upregulated in bone marrow in response to erythropoietin (EPO) treatment.
  
  
  CONCLUSIONS
  Emp expression correlates with erythroblastic island formation and has an important function for bone marrow hematopoiesis. Emp could be a potential biomarker for hematopoietic evaluation of HSCT patients.
authors.affiliation: Department of Clinical Laboratory, The Central Hospital of Wuhan, Wuhan 430014, China.
authors.forename: Xiaolu
authors.initials: X
authors.lastname: Mao
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Shi
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.forename: Guining
authors.initials: G
authors.lastname: Li
authors.forename: Lihua
authors.initials: L
authors.lastname: Hu
mesh_terms: D000293:Adolescent; D000328:Adult; D001853:Bone Marrow / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D048430:Cell Shape / Q000187:drug effects; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism*; D004900:Erythroblasts / Q000187:drug effects / Q000378:metabolism*; D004921:Erythropoietin / Q000008:administration & dosage / Q000494:pharmacology; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D006965:Hyperplasia; D007165:Immunosuppression Therapy; D008297:Male; D055815:Young Adult
chemical_list: D015815:Cell Adhesion Molecules; D003598:Cytoskeletal Proteins; C115214:MAEA protein, human; D004921:Erythropoietin