title: Decreased expression of CD9 in bovine oocytes after cryopreservation and the relationship to fertilization capacity.
abstract: This study was conducted to investigate the effect of vitrification of bovine metaphase-II (MII) oocytes on CD9 expression and fertilization capacity. Surviving vitrified/warmed oocytes were used to detect CD9 distribution (fluorescence microscopy), CD9 mRNA (qRT-PCR), and CD9 protein expression (Western blot), and to analyze in vitro fertilization rates (number of sperm bound to or that penetrated the oocytes) after removing the zona pellucida. Fresh oocytes acted as control. The experimental results showed that the vitrification/warming procedures significantly decreased CD9 expression at the mRNA and protein levels, and changed the CD9 distribution pattern in bovine oocytes. After fertilization in vitro, the average number of sperm binding and penetration of vitrified oocytes were significantly lower than those of the non-vitrified oocytes. In conclusion, vitrification of bovine oocytes caused a decrease in CD9 expression at the mRNA and protein levels, and an alteration of CD9 distribution pattern, which may have resulted in lowered fertilization capacity.
authors.affiliation: Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University (Chengdu Campus), Wenjiang, PR China. zguangbin@sicau.edu.cn
authors.forename: Guang-Bin
authors.initials: GB
authors.lastname: Zhou
authors.forename: Yan
authors.initials: Y
authors.lastname: Zeng
authors.forename: Qing-Gang
authors.initials: QG
authors.lastname: Meng
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Yun-Ping
authors.initials: YP
authors.lastname: Dai
authors.forename: Shi-En
authors.initials: SE
authors.lastname: Zhu
authors.forename: Thomas D
authors.initials: TD
authors.lastname: Bunch
authors.forename: Yun-Peng
authors.initials: YP
authors.lastname: Hou
mesh_terms: D000818:Animals; D002417:Cattle; D015925:Cryopreservation; D005260:Female; D005298:Fertility / Q000502:physiology*; D005307:Fertilization in Vitro / Q000662:veterinary; D008856:Microscopy, Fluorescence; D009865:Oocytes / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D013084:Sperm-Ovum Interactions / Q000502:physiology; D016019:Survival Analysis; D060245:Tetraspanin 29 / Q000032:analysis / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism; D058989:Vitrification
chemical_list: D060245:Tetraspanin 29