title: Neotobrilus nicsmolae n. sp. (Tobrilidae: Nematoda) and Chronogaster carolinensis n. sp. (Chronogasteridae: Nematoda) from Lake Phelps, North Carolina.
abstract: Two new species, Neotobrilus nicsmolae n. sp. and Chronogaster carolinensis n. sp. are described from a small, acidic, temperate, natural lake in North Carolina. N. nicsmolae n. sp. comes close to three members of the genus reported from North America, N. filipjevi, N. longus, and N. hopei. However, N. nicsmolae is unique with in the genus in having a combination of characters: size smaller than 1,700 μm, shorter outer labial and cephalic setae, tail shorter than 250 μm, last ventromedian supplement close (about 5 μm) to cloacal opening, spicule length of 61 to 85 μm, flagelloid sperm, and possession of subterminal setae. Assessment of relationships among clades within the Triplonchida using DNA sequences of the D2D3 expansion segment of the LSU rDNA showed that the family Trichodoridae and the genus Tripyla were recovered as monophyletic. The genus Tobrilus was recovered as monophyletic in the neighbor-joining and maximum likelihood trees, but that was not so in the maximum-parsimony tree. The separation among genera of the Trichodoridae, i.e., Trichodorus and Paratrichodorus, was not clear-cut in all phylograms. Chronogaster carolinensis n. sp. in having one ventral mucro with no spine and vacuolated lateral glandular bodies comes close to C. typica and C. ethiopica but differs from all hitherto known species in a combination of characteristics: in having long cephalic setae, long stoma, crystalloid bodies, vacuolated lateral glandular bodies, and a tail terminus with blunt ventral mucro, and its lack of lateral line.
authors.affiliation: Department of Biology and Marine Environmental Science, Elizabeth City State University, 1704 Weeksville Rd., Elizabeth City, NC.
authors.forename: Eyualem
authors.initials: E
authors.lastname: Abebe
authors.forename: Briana
authors.initials: B
authors.lastname: Ferebee
authors.forename: Tarreyca
authors.initials: T
authors.lastname: Taylor
authors.forename: Manuel
authors.initials: M
authors.lastname: Mundo-Ocampo
authors.forename: Tesfamariam
authors.initials: T
authors.lastname: Mekete
authors.forename: Paul
authors.initials: P
authors.lastname: De Ley
keywords: Free-living nematode; freshwater nematode; new species; taxonomy