title: In vivo visualization of dermal collagen fiber in skin burn by collagen-sensitive second-harmonic-generation microscopy.
abstract: Optical assessment of skin burns is possible with second-harmonic-generation (SHG) microscopy due to its high sensitivity to thermal denaturation of collagen molecules. In contrast to previous studies that were performed using excised tissue specimens ex vivo, in vivo observation of dermal collagen fibers in living rat burn models with SHG microscopy is demonstrated. Changes in signal vanishing patterns in the SHG images are confirmed to be dependent on the burn degree. Comparison of the SHG images with Masson's trichrome-stained images indicated that the observed patterns were caused by the coexistence of molten and fibrous structures of dermal collagen fibers. Furthermore, a quantitative parameter for burn assessment based on the depth profile of the mean SHG intensity across the entire SHG image is proposed. These results and discussions imply a potential of SHG microscopy as a minimally invasive, highly quantitative tool for skin burn assessment.
authors.affiliation: Osaka University, Graduate School of Engineering Science, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Tanaka
authors.forename: Shu-ichiro
authors.initials: S
authors.lastname: Fukushima
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Sasaki
authors.forename: Yuji
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Murota
authors.forename: Takeshi
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Kenji
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Araki
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yasui
mesh_terms: D000818:Animals; D002056:Burns / Q000175:diagnosis* / Q000473:pathology; D003094:Collagen / Q000737:chemistry*; D020405:Dermis / Q000473:pathology; D008297:Male; D008543:Melanins / Q000378:metabolism; D008853:Microscopy / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D012867:Skin / Q000473:pathology*
chemical_list: D008543:Melanins; D003094:Collagen