title: Enhanced antioxidant and protective activities on retinal ganglion cells of carotenoids-overexpressing transgenic carrot.
abstract: Carotenoids are considered to act as antioxidants and protect humans from serious disorders such as skin degeneration and ageing, cardiovascular disease, certain types of cancer, and age-related diseases of the eye. In this study, these chemopreventive activities of a carotenoids-overexpressing transgenic carrot were evaluated. The results of DPPH, hydroxyl, and superoxide radical scavenging tests demonstrate that the acetone extract obtained from the taproots of the carrot plants exhibits significant antioxidant activity. A higher activity was detected in the transgenic carrot extract compared with the wild-type extract. A chemopreventive activity test for degenerative diseases of the eye revealed that pretreatment with the carrot extract reduced cell death in a retinal ganglion cell line, RGC-5 cells exposed to 1-buthionine- (R,S)-sulfoximine and L-glutamic acid.
authors.affiliation: College of Pharmacy, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Kee Dong
authors.initials: KD
authors.lastname: Yoon
authors.forename: Suk-Nam
authors.initials: SN
authors.lastname: Kang
authors.forename: Ji-Yeong
authors.initials: JY
authors.lastname: Bae
authors.forename: Haeng-Soon
authors.initials: HS
authors.lastname: Lee
authors.forename: Sang-Soo
authors.initials: SS
authors.lastname: Kwak
authors.forename: Insurk
authors.initials: I
authors.lastname: Jang
authors.forename: Il-Suk
authors.initials: IS
authors.lastname: Kim
authors.forename: Cheol Ho
authors.initials: CH
authors.lastname: Lee
authors.forename: Jung Myung
authors.initials: JM
authors.lastname: Bae
authors.forename: Shin Woo
authors.initials: SW
authors.lastname: Lee
authors.forename: Mi-Jeong
authors.initials: MJ
authors.lastname: Ahn
mesh_terms: D000096:Acetone; D000975:Antioxidants / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D001713:Biphenyl Compounds; D019328:Buthionine Sulfoximine / Q000494:pharmacology; D002338:Carotenoids / Q000032:analysis / Q000494:pharmacology*; D016923:Cell Death; D002460:Cell Line; D018552:Daucus carota / Q000235:genetics* / Q000378:metabolism; D018698:Glutamic Acid / Q000494:pharmacology; D006801:Humans; D017665:Hydroxyl Radical / Q000378:metabolism; D018384:Oxidative Stress; D010851:Picrates; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D020011:Protective Agents / Q000494:pharmacology*; D012165:Retinal Ganglion Cells / Q000187:drug effects* / Q000502:physiology; D013481:Superoxides / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; D010936:Plant Extracts; D020011:Protective Agents; D013481:Superoxides; D000096:Acetone; D017665:Hydroxyl Radical; D002338:Carotenoids; D018698:Glutamic Acid; D019328:Buthionine Sulfoximine; C004931:1,1-diphenyl-2-picrylhydrazyl