title: MicroRNA-34a inhibits human osteosarcoma proliferation by downregulating ether à go-go 1 expression.
abstract: Aberrant expression of MicroRNAs (miRNAs) has been implicated in several types of cancer. As a direct target gene of p53, miR-34a has been suggested to mediate the tumor suppressor function of p53. Ether à go-go 1 (Eag1) channel is overexpressed in a variety of cancers and plays important roles in cancer progression. However, the link between miR-34a and Eag1 in cancer is unclear. In this study, we used human osteosarcoma as the model to demonstrate that miR-34a was significantly downregulated in osteosarcoma tissues and cell lines compared with normal brain tissues and osteoblastic cell line. Next we evaluated the role of miR-34a in the regulation of osteosarcoma cell proliferation by CCK-8 and colony formation assays. The results showed that overexpression of miR-34a inhibited the proliferation of MG-63 and Saos-2 cells. Furthermore, xenograft nude mice model showed that miR-34a inhibited osteosarcoma growth in vivo. Mechanistically, we found that overexpression of miR-34a led to decreased Eag1 expression in osteosarcoma cells while inhibition of miR-34a increased Eag1 expression. Taken together, our results suggest that miR-34a could inhibit osteosarcoma growth via the down regulation of Eag1 expression.
authors.affiliation: Department of Neurology, the Affiliated Southeast Hospital of Xiamen University, Zhangzhou 363000, China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Wu
authors.forename: Daixing
authors.initials: D
authors.lastname: Zhong
authors.forename: Quan
authors.initials: Q
authors.lastname: Gao
authors.forename: Wenliang
authors.initials: W
authors.lastname: Zhai
authors.forename: Zhenqi
authors.initials: Z
authors.lastname: Ding
authors.forename: Jin
authors.initials: J
authors.lastname: Wu
mesh_terms: D000818:Animals; D001859:Bone Neoplasms / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D051638:Ether-A-Go-Go Potassium Channels / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology; D014183:Transplantation, Heterologous; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism
chemical_list: D051638:Ether-A-Go-Go Potassium Channels; C496884:KCNH1 protein, human; C522425:MIRN34 microRNA, human; D035683:MicroRNAs; D016159:Tumor Suppressor Protein p53
keywords: Ether à go-go1 (Eag1); MicroRNA-34a; osteosarcoma; proliferation; target gene.