title: Dermal mesenchymal stem cells (DMSCs) inhibit skin-homing CD8+ T cell activity, a determining factor of vitiligo patients' autologous melanocytes transplantation efficiency.
abstract: We here investigated the efficiency of autologous melanocyte transplantation of 23 vitiligo patients by focusing on perilesional skin homing CD8+ T lymphocytes, and studied the potential effect of dermal mesenchymal stem cells (DMSCs) on CD8+ T cell activities in vitro. Out of 23 patients with the autologous melanocyte transplantation, 12 patients (52.17%) had an excellent re-pigmentation, 6 patients (26.09%) had a good re-pigmentation, 5 patients (21.74%) had a fair or poor re-pigmentation. CD8+ T cells infiltrating was observed in the perilesional vitiligo area of all patients. Importantly, the efficiency of the transplantation was closely associated with skin-homing CD8+ T cell activities. The patients with high number of perilesional CD8+ T cells or high level of cytokines/chemokines were associated with poor re-pigmentation efficiency. For in-vitro experiments, we successfully isolated and characterized human DMSCs and skin-homing CD8+ T cells. We established DMSCs and CD8+ T cell co-culture system, where DMSCs possessed significant inhibitory effects against skin homing CD8+ T lymphocytes. DMSCs inhibited CD8+ T cells proliferation, induced them apoptosis and regulated their cytokines/chemokines production. Our results suggest that vitiligo patients' autologous melanocytes transplantation efficiency might be predicted by perilesional skin-homing CD8+ T cell activities, and DMSCs might be used as auxiliary agent to improve transplantation efficacy.
authors.affiliation: Department of Dermatology, The Third People's Hospital of Hangzhou, Hangzhou, PR China.
authors.forename: Miao-ni
authors.initials: MN
authors.lastname: Zhou
authors.forename: Zhi-qing
authors.initials: ZQ
authors.lastname: Zhang
authors.forename: Ji-long
authors.initials: JL
authors.lastname: Wu
authors.forename: Fu-quan
authors.initials: FQ
authors.lastname: Lin
authors.forename: Li-fang
authors.initials: LF
authors.lastname: Fu
authors.forename: Sui-qan
authors.initials: SQ
authors.lastname: Wang
authors.forename: Cui-ping
authors.initials: CP
authors.lastname: Guan
authors.forename: Hong-lin
authors.initials: HL
authors.lastname: Wang
authors.forename: Aie
authors.initials: A
authors.lastname: Xu
mesh_terms: D000328:Adult; D017209:Apoptosis; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000473:pathology; D049109:Cell Proliferation; D017690:Cell Transplantation; D018925:Chemokines / Q000096:biosynthesis / Q000378:metabolism; D004817:Epidermis / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology; D006801:Humans; D008297:Male; D008544:Melanocytes / Q000637:transplantation*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010858:Pigmentation / Q000276:immunology; D012867:Skin / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D014182:Transplantation, Autologous; D014820:Vitiligo / Q000276:immunology / Q000378:metabolism / Q000601:surgery*
chemical_list: D018925:Chemokines