title: Pepino mosaic virus genotype shift in North America and development of a loop-mediated isothermal amplification for rapid genotype identification.
abstract: BACKGROUND
  Pepino mosaic, once an emerging disease a decade ago, has become endemic on greenhouse tomatoes worldwide in recent years. Three distinct genotypes of Pepino mosaic virus (PepMV), including EU, US1 and CH2 have been recognized. Our earlier study conducted in 2006-2007 demonstrated a predominant EU genotype in Canada and United States. The objective of the present study was to monitor the dynamic of PepMV genetic composition and its current status in North America.
  
  
  RESULTS
  Through yearly monitoring efforts in 2009-2012, we detected a dramatic shift in the prevalent genotype of PepMV from the genotype EU to CH2 in North America since early 2010, with another shift from CH2 to US1 occurring in Mexico only two years later. Through genetic diversity analysis using the coat protein gene, such genotype shifting of PepMV in North America was linked to the positive identification of similar sequence variants in two different commercial tomato seed sources used for scion and rootstock, respectively. To allow for a quick identification, a reverse transcription loop-mediated isothermal amplification (RT-LAMP) system was developed and demonstrated to achieve a rapid identification for each of the three genotypes of PepMV, EU, US1 and CH2.
  
  
  CONCLUSIONS
  Through systemic yearly monitoring and genetic diversity analysis, we identified a linkage between the field epidemic isolates and those from commercial tomato seed lots as the likely sources of initial PepMV inoculum that resulted in genetic shifting as observed on greenhouse tomatoes in North America. Application of the genotype-specific RT-LAMP system would allow growers to efficiently determine the genetic diversity on their crops.
authors.affiliation: U.S. Department of Agriculture-Agricultural Research Service, U.S. Vegetable Laboratory, Charleston, SC 29414, USA. kai.ling@ars.usda.gov
authors.forename: Kai-Shu
authors.initials: KS
authors.lastname: Ling
authors.forename: Rugang
authors.initials: R
authors.lastname: Li
authors.forename: Michael
authors.initials: M
authors.lastname: Bledsoe
mesh_terms: D014644:Genetic Variation; D005838:Genotype; D018551:Solanum lycopersicum / Q000821:virology; D008969:Molecular Sequence Data; D009656:North America; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D010935:Plant Diseases / Q000821:virology; D017863:Potexvirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D014773:Virology / Q000379:methods*