title: Rhodamine-based 'turn-on' fluorescent probe for Cu(II) and its fluorescence imaging in living cells.
abstract: A novel rhodamine spirolactam derivative 3',6'-Bis(diethylamino)-2-(2-hydroxyethylamino) spiro[isoindoline-1,9'-xanthen]-3-one (RO1) was synthesized, and characterized by high-resolution mass spectrometry (HRMS), X-ray crystallography, Infrared spectroscopy (IR), and (1)H NMR and (13)C NMR spectroscopy. RO1 exhibited highly sensitive and exclusively selective fluorescence response toward Cu(2+) over other metal ions with a detection limit of 0.56ppb in mixed aqueous solution. The fluorescence was pH-independent in the wide range pH 3.1-11.6. The turn-on fluorescence enhancement of the probe is based on Cu(2+) induced ring-opening mechanism of the rhodamine spirolactam. Moreover, by means of fluorescence microscopy experiments, it was demonstrated that RO1 could monitor trace Cu(2+) changes by live cell imaging.
authors.affiliation: State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Rd., Hi-tech Zone, Dalian 116024, People's Republic of China.
authors.forename: Mao-Zhong
authors.initials: MZ
authors.lastname: Tian
authors.forename: Ming-Ming
authors.initials: MM
authors.lastname: Hu
authors.forename: Jiang-Li
authors.initials: JL
authors.lastname: Fan
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Peng
authors.forename: Jing-Yun
authors.initials: JY
authors.lastname: Wang
authors.forename: Shi-Guo
authors.initials: SG
authors.lastname: Sun
authors.forename: Rong
authors.initials: R
authors.lastname: Zhang
mesh_terms: D003300:Copper / Q000032:analysis*; D018360:Crystallography, X-Ray; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008856:Microscopy, Fluorescence; D008958:Models, Molecular; D015394:Molecular Structure; D012235:Rhodamines / Q000138:chemical synthesis / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D012235:Rhodamines; D003300:Copper