title: Trauma-associated human neutrophil alterations revealed by comparative proteomics profiling.
abstract: OBJECTIVE
  Polymorphonuclear neutrophils (PMNs) play an important role in mediating the innate immune response after severe traumatic injury; however, the cellular proteome response to traumatic condition is still largely unknown.
  
  
  METHODS
  We applied 2D-LC-MS/MS-based shotgun proteomics to perform comparative proteome profiling of human PMNs from severe trauma patients and healthy controls.
  
  
  RESULTS
  A total of 197 out of ~2500 proteins (being identified with at least two peptides) were observed with significant abundance changes following the injury. The proteomics data were further compared with transcriptomics data for the same genes obtained from an independent patient cohort. The comparison showed that the protein abundance changes for the majority of proteins were consistent with the mRNA abundance changes in terms of directions of changes. Moreover, increased protein secretion was suggested as one of the mechanisms contributing to the observed discrepancy between protein and mRNA abundance changes. Functional analyses of the altered proteins showed that many of these proteins were involved in immune response, protein biosynthesis, protein transport, NRF2-mediated oxidative stress response, the ubiquitin-proteasome system, and apoptosis pathways.
  
  
  CONCLUSIONS
  Our data suggest increased neutrophil activation and inhibited neutrophil apoptosis in response to trauma. The study not only reveals an overall picture of functional neutrophil response to trauma at the proteome level, but also provides a rich proteomics data resource of trauma-associated changes in the neutrophil that will be valuable for further studies of the functions of individual proteins in PMNs.
authors.affiliation: Biological Sciences Division and Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA 99352, USA.
authors.forename: Jian-Ying
authors.initials: JY
authors.lastname: Zhou
authors.forename: Ravi K
authors.initials: RK
authors.lastname: Krovvidi
authors.forename: Yuqian
authors.initials: Y
authors.lastname: Gao
authors.forename: Hong
authors.initials: H
authors.lastname: Gao
authors.forename: Brianne O
authors.initials: BO
authors.lastname: Petritis
authors.forename: Asit K
authors.initials: AK
authors.lastname: De
authors.forename: Carol L
authors.initials: CL
authors.lastname: Miller-Graziano
authors.forename: Paul E
authors.initials: PE
authors.lastname: Bankey
authors.forename: Vladislav A
authors.initials: VA
authors.lastname: Petyuk
authors.forename: Carrie D
authors.initials: CD
authors.lastname: Nicora
authors.forename: Therese R
authors.initials: TR
authors.lastname: Clauss
authors.forename: Ronald J
authors.initials: RJ
authors.lastname: Moore
authors.forename: Tujin
authors.initials: T
authors.lastname: Shi
authors.forename: Joseph N
authors.initials: JN
authors.lastname: Brown
authors.forename: Amit
authors.initials: A
authors.lastname: Kaushal
authors.forename: Wenzhong
authors.initials: W
authors.lastname: Xiao
authors.forename: Ronald W
authors.initials: RW
authors.lastname: Davis
authors.forename: Ronald V
authors.initials: RV
authors.lastname: Maier
authors.forename: Ronald G
authors.initials: RG
authors.lastname: Tompkins
authors.forename: Wei-Jun
authors.initials: WJ
authors.lastname: Qian
authors.forename: David G
authors.initials: DG
authors.lastname: Camp
authors.forename: Richard D
authors.initials: RD
authors.lastname: Smith
mesh_terms: D017209:Apoptosis; D016022:Case-Control Studies; D006801:Humans; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D009504:Neutrophils / Q000276:immunology / Q000378:metabolism*; D018384:Oxidative Stress; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D014176:Protein Biosynthesis; D021381:Protein Transport; D040901:Proteomics; D015398:Signal Transduction; D059467:Transcriptome; D025801:Ubiquitin / Q000378:metabolism; D014947:Wounds and Injuries / Q000235:genetics / Q000276:immunology* / Q000378:metabolism* / Q000473:pathology
chemical_list: D051267:NF-E2-Related Factor 2; C495635:NFE2L2 protein, human; D025801:Ubiquitin; D046988:Proteasome Endopeptidase Complex
keywords: Genomics; Human neutrophil; LC-MS/MS; Trauma