title: Quartz-Seq: a highly reproducible and sensitive single-cell RNA sequencing method, reveals non-genetic gene-expression heterogeneity.
abstract: Development of a highly reproducible and sensitive single-cell RNA sequencing (RNA-seq) method would facilitate the understanding of the biological roles and underlying mechanisms of non-genetic cellular heterogeneity. In this study, we report a novel single-cell RNA-seq method called Quartz-Seq that has a simpler protocol and higher reproducibility and sensitivity than existing methods. We show that single-cell Quartz-Seq can quantitatively detect various kinds of non-genetic cellular heterogeneity, and can detect different cell types and different cell-cycle phases of a single cell type. Moreover, this method can comprehensively reveal gene-expression heterogeneity between single cells of the same cell type in the same cell-cycle phase.
authors.forename: Yohei
authors.initials: Y
authors.lastname: Sasagawa
authors.forename: Itoshi
authors.initials: I
authors.lastname: Nikaido
authors.forename: Tetsutaro
authors.initials: T
authors.lastname: Hayashi
authors.forename: Hiroki
authors.initials: H
authors.lastname: Danno
authors.forename: Kenichiro D
authors.initials: KD
authors.lastname: Uno
authors.forename: Takeshi
authors.initials: T
authors.lastname: Imai
authors.forename: Hiroki R
authors.initials: HR
authors.lastname: Ueda
mesh_terms: D000818:Animals; D002460:Cell Line; D051379:Mice; D009928:Organ Specificity; D012680:Sensitivity and Specificity; D017423:Sequence Analysis, RNA / Q000379:methods*; D059010:Single-Cell Analysis / Q000379:methods*; D014158:Transcription, Genetic