title: Mailuoning prevents high-glucose-mediated human umbilical vein endothelial cells apoptosis.
abstract: OBJECTIVE
  To investigated the role of Mailuoning in the prevention of high-glucose-mediated cell apoptosis in human umbilical vein endothelial cells (HUVEC) and the mechanisms involved.
  
  
  METHODS
  MTT assay was used to investigate cell viability, western blot was used to investigate protein expression, and flow cytometric detection technology was used to detect cell apoptosis.
  
  
  RESULTS
  Exposure of HUVEC to high glucose (50 mM) significantly suppressed cell viability and increased cell apoptosis compared with normal glucose (11 mM) (all P < 0.05). However, Mailuoning prevented high-glucose-induced HUVEC apoptosis in dose-dependent manner. Further studies indicated that Mailuoning suppressed high-glucose-induced p38 mitogen-activated protein kinase phosphorylation, but had no effect on extracellular signal-regulated kinase 1/2 and Akt phosphorylation.
  
  
  CONCLUSIONS
  Mailuoning can prevent high-glucose-induced HUVEC apoptosis by suppressing p38 activation.
authors.affiliation: Department of Ophthalmonogy, Jiangsu Province Hospital on Integration of Chinese and Western Medicine, Nanjing 210028, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Xueting
authors.initials: X
authors.lastname: Cai
authors.forename: Wuguang
authors.initials: W
authors.lastname: Lu
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Gu
authors.forename: Peng
authors.initials: P
authors.lastname: Cao
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005947:Glucose / Q000633:toxicity*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D015398:Signal Transduction / Q000187:drug effects; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D048051:p38 Mitogen-Activated Protein Kinases; D005947:Glucose