title: First isolation of Aedes flavivirus in the Western Hemisphere and evidence of vertical transmission in the mosquito Aedes (Stegomyia) albopictus (Diptera: Culicidae).
abstract: We report here the first evidence of vertical transmission of Aedes flavivirus (AEFV) and its first isolation in the Western Hemisphere. AEFV strain SPFLD-MO-2011-MP6 was isolated in C6/36 cells from a pool of male Aedes albopictus mosquitoes that were reared to adults from larvae collected in southwest Missouri, USA, in 2011. Electron micrographs of the virus showed virions of approximately 45nm in diameter with morphological characteristics associated with flaviviruses. The genomic sequence demonstrated that AEFV-SPFLD-MO-2011-MP6 shares a high degree of nucleotide and amino acid sequence identity with the AEFV Narita-21 strain, isolated in Japan in 2003. Intracerebral inoculation of newborn mice with the virus failed to produce observable illness or death and the virus did not replicate in vertebrate cells, consistent with a lack of vertebrate host range.
authors.affiliation: Institute for Human Infections and Immunity, Center for Emerging Infectious Diseases and Biodefense, Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555-0609, USA. adhaddow@gmail.com
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Haddow
authors.forename: Hilda
authors.initials: H
authors.lastname: Guzman
authors.forename: Vsevolod L
authors.initials: VL
authors.lastname: Popov
authors.forename: Thomas G
authors.initials: TG
authors.lastname: Wood
authors.forename: Steven G
authors.initials: SG
authors.lastname: Widen
authors.forename: Alastair D
authors.initials: AD
authors.lastname: Haddow
authors.forename: Robert B
authors.initials: RB
authors.lastname: Tesh
authors.forename: Scott C
authors.initials: SC
authors.lastname: Weaver
mesh_terms: D000330:Aedes / Q000821:virology*; D000818:Animals; D002460:Cell Line; D004195:Disease Models, Animal; D005416:Flavivirus / Q000302:isolation & purification* / Q000472:pathogenicity / Q000648:ultrastructure; D018177:Flavivirus Infections / Q000473:pathology / Q000821:virology; D008297:Male; D051379:Mice; D046529:Microscopy, Electron, Transmission; D008923:Missouri; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014776:Virus Cultivation
chemical_list: D012367:RNA, Viral