title: The effect of porosity of a biphasic ceramic scaffold on human skeletal stem cell growth and differentiation in vivo.
abstract: Skeletal stem cell (SSC) growth on a novel porous HA/TCP scaffold has been investigated in vivo. The effect of porosity on osteogenic differentiation was assessed by comparing two groups of scaffolds with differing porosity but controlled pore size. Histology, microCT, scanning electron microscopy, and biochemical analysis were used to assess SSC proliferation and differentiation. The 45 pores per inch (ppi) scaffold demonstrated a greater increase in density than the 30 ppi scaffold following in vivo culture, and a reduction in dimensions of the pores and channels of the higher porosity scaffold was observed, indicating generation of new tissue within the pores. All scaffolds supported SSC proliferation but the higher scaffold porosity augmented osteogenic differentiation. ALP specific activity was enhanced on the 45 ppi scaffold compared to the 30 ppi scaffold. These studies demonstrate the importance of porosity in scaffold design and impact therein for tissue engineering application.
authors.affiliation: Bone and Joint Research Group, Centre for Human Development, Stem Cell and Regeneration, Institute of Developmental Sciences, University of Southampton, Tremona Road, Southampton SO16 6YD, United Kingdom.
authors.forename: Alexander
authors.initials: A
authors.lastname: Aarvold
authors.forename: James O
authors.initials: JO
authors.lastname: Smith
authors.forename: Edward R
authors.initials: ER
authors.lastname: Tayton
authors.forename: Stuart A
authors.initials: SA
authors.lastname: Lanham
authors.forename: Julian B
authors.initials: JB
authors.lastname: Chaudhuri
authors.forename: Irene G
authors.initials: IG
authors.lastname: Turner
authors.forename: Richard O C
authors.initials: RO
authors.lastname: Oreffo
mesh_terms: D000328:Adult; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002516:Ceramics / Q000494:pharmacology*; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008855:Microscopy, Electron, Scanning; D018482:Muscle, Skeletal / Q000166:cytology*; D016062:Porosity; D013194:Staining and Labeling; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D054457:Tissue Scaffolds / Q000737:chemistry*; D055114:X-Ray Microtomography
keywords: osteogenic differentiation; porosity; proliferation; scaffold; skeletal stem cell