title: Ganoderma lucidumpolysaccharides counteract inhibition on CD71 and FasL expression by culture supernatant of B16F10 cells upon lymphocyte activation.
abstract: Immune responses to tumor-associated antigens are often detectable in tumor-bearing hosts, but they fail to eliminate malignant cells or prevent development of metastases. Tumor cells produce factors such as interleukin-10, transforming growth factor-β1 and vascular endothelial growth factor (VEGF) that suppress the function of immune cells or induce apoptosis of immune cells. Culture supernatant of tumor cells may contain these immunosuppressive factors which suppress lymphocyte activation. CD71 and FasL are two important molecules that are expressed upon lymphocyte activation. Counteraction against suppression CD71 and FasL expression upon lymphocyte activation may benefit tumor control. A potential component with this effect isGanoderma lucidumpolysaccharides (Gl-PS). In this study,Gl-PS was used on lymphocytes incubating with culture supernatant of B16F10 melanoma cells (B16F10-CS) in the presence of phytohemagglutinin. Following induction with phytohemagglutinin, B16F10-CS suppressed CD71 expression in lymphocytes (as detected by immunofluorescence and flow cytometry), proliferation in lymphocytes (as detected by MTT assay), and FasL expression in lymphocytes (as detected by immunocytochemistry and western blot analysis), whileGl-PS fully or partially counteracted these suppressions.Gl-PS showed counteractive effects against suppression induced by B16F10-CS on CD71 and FasL expression upon lymphocyte activation, suggesting the potential ofGl-PS to facilitate cancer immunotherapy.
authors.affiliation: The Affiliated Hospital of Chengde Medical College, Chengde, Hebei 067000;
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: Sun
authors.forename: Zhi-Bin
authors.initials: ZB
authors.lastname: Lin
authors.forename: Xin-Suo
authors.initials: XS
authors.lastname: Duan
authors.forename: Jie
authors.initials: J
authors.lastname: Lu
authors.forename: Zhi-Hua
authors.initials: ZH
authors.lastname: Ge
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.forename: En-Hong
authors.initials: EH
authors.lastname: Xing
authors.forename: Tian-Fei
authors.initials: TF
authors.lastname: Lan
authors.forename: Miao-Miao
authors.initials: MM
authors.lastname: Jiang
authors.forename: Ning
authors.initials: N
authors.lastname: Yang
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Li
keywords: CD71; FasL; Ganoderma lucidum; lymphocyte activation; polysaccharides; tumor