title: A personal reflection on the replicon theory: from R1 plasmid to replication timing regulation in human cells.
abstract: Fifty years after the Replicon Theory was originally presented, detailed mechanistic insight into prokaryotic replicons has been obtained and rapid progress is being made to elucidate the more complex regulatory mechanisms of replicon regulation in eukaryotic cells. Here, I present my personal perspectives on how studies of model replicons have contributed to our understanding of the basic mechanisms of DNA replication as well as the evolution of replication regulation in human cells. I will also discuss how replication regulation contributes to the stable maintenance of the genome and how disruption of replication regulation leads to human diseases.
authors.affiliation: Department of Genome Medicine, Tokyo Metropolitan Institute of Medical Science, 2-1-6 Kamkitazawa, Setagaya-ku, Tokyo 156-8506, Japan. Electronic address: masai-hs@igakuken.or.jp.
authors.forename: Hisao
authors.initials: H
authors.lastname: Masai
mesh_terms: D001419:Bacteria / Q000235:genetics*; D004261:DNA Replication; D004194:Disease / Q000235:genetics; D042822:Genomic Instability; D049673:History, 20th Century; D049674:History, 21st Century; D006801:Humans; D008967:Molecular Biology / Q000266:history / Q000639:trends; D012093:Replicon; D013762:Tetrahydrofolate Dehydrogenase
chemical_list: C006094:R388; D013762:Tetrahydrofolate Dehydrogenase
keywords: R1 plasmid; Replicon Theory; initiator; replication origin; replication timing