title: Polystyrene spheres-assisted matrix-assisted laser desorption ionization mass spectrometry for quantitative analysis of plasma lysophosphatidylcholines.
abstract: The quantitative analysis by matrix-assisted laser desorption ionization mass spectrometry (MALDI MS) is a challenge due to the poor reproducibility originating from the heterogeneity of the matrix-analyte crystals. Polystyrene (PS) colloidal spheres have superior monodispersed property and can self-assemble to form photonic crystals. With the assistance of PS spheres, a uniform matrix-analyte cocrystal was constructed for the quantitative analysis of plasma lysophosphatidylcholines (LPCs). The matrix and the solvent in MALDI MS analysis were optimized, and the reproducibility and the accuracy were investigated in detail. This is the first report about the very simple method of PS spheres-assisted MALDI MS for quantitative analysis, where it is believed that this approach will greatly expand the applications of MALDI MS.
authors.affiliation: Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry Chinese Academy of Science, Beijing 100190, China.
authors.forename: Yanbo
authors.initials: Y
authors.lastname: Wei
authors.forename: Shumu
authors.initials: S
authors.lastname: Li
authors.forename: Jingxia
authors.initials: J
authors.lastname: Wang
authors.forename: Chunying
authors.initials: C
authors.lastname: Shu
authors.forename: Jian'an
authors.initials: J
authors.lastname: Liu
authors.forename: Shaoxiang
authors.initials: S
authors.lastname: Xiong
authors.forename: Jianwen
authors.initials: J
authors.lastname: Song
authors.forename: Junjie
authors.initials: J
authors.lastname: Zhang
authors.forename: Zhenwen
authors.initials: Z
authors.lastname: Zhao
mesh_terms: D000236:Adenoma / Q000097:blood*; D015179:Colorectal Neoplasms / Q000097:blood*; D006801:Humans; D008244:Lysophosphatidylcholines / Q000097:blood*; D011137:Polystyrenes / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D008244:Lysophosphatidylcholines; D011137:Polystyrenes