title: Preclinical efficacy of slow-release bFGF in ischemia-reperfusion injury in a Dorsal Island skin flap model.
abstract: The effect of slow-release basic fibroblast growth factor (bFGF) on ischemia-reperfusion injury was examined using an island skin flap model in rats. Paired rectangular island skin flaps were elevated on the dorsum of 30 Fischer rats. The flaps were subjected to 6 hours of ischemia. Before reperfusion the flaps were injected with acidic gelatin hydrogel microspheres + phosphate-buffered saline (PBS) (group I), 20 μg slow-release bFGF + PBS (group II), 50 μg slow-release bFGF + PBS (group III), and 150 μg slow-release bFGF + PBS (group IV). The mean percent flap survival area and the average number of vessels detected by microangiography were significantly higher in group IV (p < 0.05) than in groups I, II, and III. The immunohistochemical staining for vasculogenic growth factors was quantitatively higher in group IV (p < 0.01). In conclusion, slow-release bFGF prevents ischemia-reperfusion injury by upregulating the secretion of vasculogenic growth factors.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Nippon Medical School, Tokyo, Japan.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Hakan
authors.initials: H
authors.lastname: Orbay
authors.forename: Chenyu
authors.initials: C
authors.lastname: Huang
authors.forename: Morikuni
authors.initials: M
authors.lastname: Tobita
authors.forename: Hiko
authors.initials: H
authors.lastname: Hyakusoku
authors.forename: Masaaki
authors.initials: M
authors.lastname: Myamoto
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Tabata
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mizuno
mesh_terms: D000792:Angiography / Q000379:methods; D000818:Animals; D016207:Cytokines / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D006085:Graft Survival; D007124:Immunoenzyme Techniques; D051381:Rats; D011916:Rats, Inbred F344; D015427:Reperfusion Injury / Q000378:metabolism* / Q000517:prevention & control; D013524:Surgical Flaps / Q000098:blood supply*; D013817:Thermography
chemical_list: D016207:Cytokines; D016222:Fibroblast Growth Factor 2