title: [Analysis of 89 amniotic samples using fluorescent in situ hybridization].
abstract: OBJECTIVE
  To assess the value of fluorescent in situ hybridization (FISH) for detecting common chromosome aneuploidies in interphase nuclei of amniotic fluid cells.
  
  
  METHODS
  Eighty two uncultured amniotic fluid samples and supernatants from 2 successfully and 5 unsuccessfully cultured amniotic fluid samples were analyzed with FISH. Results from standard cytogenetic analysis of 79 uncultured amniotic fluid samples and 2 successfully cultured amniotic fluid samples were compared with FISH results.
  
  
  RESULTS
  All of the 89 samples were succeeded analyzed with FISH. Positive findings included 3 cases with trisomy 21, 1 case with 47, XYY and 1 case with 69, XXX, which were consistent with results of karyotype analysis.
  
  
  CONCLUSIONS
  FISH is a rapid and accurate method for prenatal diagnosis, and can also provide a remedy to failed amniotic fluid cells culture.
authors.affiliation: Sichuan Provincial Hospital for Women and Children and Sichuan Prenatal Diagnosis Center, Chengdu, P.R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wei
authors.forename: Yun-xing
authors.initials: YX
authors.lastname: Li
authors.forename: Lan
authors.initials: L
authors.lastname: Zeng
authors.forename: Chun
authors.initials: C
authors.lastname: Chen
authors.forename: Sheng-fang
authors.initials: SF
authors.lastname: Qin
authors.forename: Xue-yan
authors.initials: XY
authors.lastname: Wang
authors.forename: Na
authors.initials: N
authors.lastname: Xi
authors.forename: Shu-qin
authors.initials: SQ
authors.lastname: Tang
authors.forename: Mei
authors.initials: M
authors.lastname: Leng
authors.forename: Chun-lan
authors.initials: CL
authors.lastname: Liu
mesh_terms: D000328:Adult; D000653:Amniotic Fluid / Q000166:cytology*; D018929:Cell Culture Techniques; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007621:Karyotyping; D011247:Pregnancy