title: Gross and true ileal digestible amino acid contents of several animal body proteins and their hydrolysates.
abstract: Amino acid compositions of ovine muscle, ovine myofibrillar protein, ovine spleen, ovine liver, bovine blood plasma, bovine blood globulins and bovine serum albumin and the amino acid compositions and in vivo (laboratory rat) true ileal amino acid digestibilities of hydrolysates (sequential hydrolysis with Neutrase, Alcalase and Flavourzyme) of these protein sources were determined. True ileal amino acid digestibility differed (P<0.05) among the seven protein hydrolysates. The ovine myofibrillar protein and liver hydrolysates were the most digestible, with a mean true ileal digestibility across all amino acids of 99%. The least digestible protein hydrolysate was bovine serum albumin with a comparable mean true ileal digestibility of 93%. When the digestible amino acid contents were expressed as proportions relative to lysine, considerable differences, across the diverse protein sources, were found in the pattern of predicted absorbed amino acids.
authors.affiliation: Riddet Institute, Massey University, Palmerston North, New Zealand. J.C.Cui@massey.ac.nz
authors.forename: J
authors.initials: J
authors.lastname: Cui
authors.forename: B
authors.initials: B
authors.lastname: Chong
authors.forename: S M
authors.initials: SM
authors.lastname: Rutherfurd
authors.forename: B
authors.initials: B
authors.lastname: Wilkinson
authors.forename: H
authors.initials: H
authors.lastname: Singh
authors.forename: P J
authors.initials: PJ
authors.lastname: Moughan
mesh_terms: D000596:Amino Acids / Q000032:analysis* / Q000378:metabolism; D000818:Animals; D002417:Cattle; D004063:Digestion; D005766:Gastrointestinal Contents / Q000737:chemistry; D005916:Globulins / Q000032:analysis / Q000378:metabolism; D007082:Ileum / Q000378:metabolism*; D008099:Liver / Q000737:chemistry; D008239:Lysine / Q000032:analysis / Q000378:metabolism; D018482:Muscle, Skeletal / Q000737:chemistry; D009210:Myofibrils / Q000737:chemistry; D011492:Protein Hydrolysates / Q000032:analysis* / Q000378:metabolism; D011506:Proteins / Q000032:analysis* / Q000378:metabolism; D051381:Rats; D012709:Serum Albumin / Q000032:analysis / Q000378:metabolism; D012756:Sheep; D013154:Spleen / Q000737:chemistry
chemical_list: D000596:Amino Acids; D005916:Globulins; D011492:Protein Hydrolysates; D011506:Proteins; D012709:Serum Albumin; D008239:Lysine