title: Proteomic analysis of vascular smooth muscle cells with S- and R-enantiomers of atenolol by iTRAQ and LC-MS/MS.
abstract: The term "proteome" was first introduced by referring to the complete determination of proteins expressed in a given cell, tissue, or organism. In HPLC-based proteomics technique, the mixture of the cleaved peptides are bonded, separated sequentially on the multidimensional columns based on charge or hydrophobicity of the ionized analytes, and then eluted into the MS for identification. Among the developed stable isotope-based quantification methods, iTRAQ has recently gained popularity as its simple iTRAQ labeling procedures and up to eight labeled samples examined in a single experiment.
authors.affiliation: School of Chemical and Biomedical Engineering, College of Engineering, Nanyang Technological University, Singapore, Singapore.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhang
authors.forename: Jiahua
authors.initials: J
authors.lastname: Shi
authors.forename: Benedict Jia Hong
authors.initials: BJ
authors.lastname: Lee
authors.forename: Liwei
authors.initials: L
authors.lastname: Chen
authors.forename: Kee Yang
authors.initials: KY
authors.lastname: Tan
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Tang
authors.forename: Jane Yi Lin
authors.initials: JY
authors.lastname: Tan
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.forename: Huixing
authors.initials: H
authors.lastname: Feng
authors.forename: Wei Ning
authors.initials: WN
authors.lastname: Chen
mesh_terms: D001262:Atenolol / Q000737:chemistry* / Q000494:pharmacology; D002460:Cell Line; D002853:Chromatography, Liquid; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D036103:Nanotechnology; D059748:Proteolysis; D040901:Proteomics / Q000379:methods*; D013223:Statistics as Topic; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry
chemical_list: D001262:Atenolol