title: CCR6 is transiently upregulated on B cells after activation and modulates the germinal center reaction in the mouse.
abstract: The CC-chemokine receptor 6 (CCR6) is expressed constitutively at an intermediate level on naïve B cells and is upregulated after activation on pregerminal center (GC) B cells. We hypothesized that it could be involved in the events leading to GC reaction and high-affinity antibody production, and therefore investigated the potential role of CCR6 in B-cell differentiation in vivo. After antigenic challenge of CCR6-/- mice with the T-cell-dependent antigen nitrophenyl-keyhole limpet hemocyanin (NP-KLH), GC B-cell development was found to be accelerated and the number of GC had increased significantly compared with control mice, but the antibodies produced by CCR6-/- B cells were on average of lower affinity. We conclude from these data that the CCR6/CCL20 axis has an important role in regulating the kinetics and efficiency of the GC reaction.
authors.affiliation: Comparative Genomics Center, James Cook University, Townsville, Queensland, Australia.
authors.forename: Florian
authors.initials: F
authors.lastname: Wiede
authors.forename: Phillip D
authors.initials: PD
authors.lastname: Fromm
authors.forename: Iain
authors.initials: I
authors.lastname: Comerford
authors.forename: Ervin
authors.initials: E
authors.lastname: Kara
authors.forename: Jennifer
authors.initials: J
authors.lastname: Bannan
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Schuh
authors.forename: Charani
authors.initials: C
authors.lastname: Ranasinghe
authors.forename: David
authors.initials: D
authors.lastname: Tarlinton
authors.forename: Thomas
authors.initials: T
authors.lastname: Winkler
authors.forename: Shaun R
authors.initials: SR
authors.lastname: McColl
authors.forename: Heinrich
authors.initials: H
authors.lastname: Körner
mesh_terms: D000818:Animals; D000915:Antibody Affinity / Q000235:genetics; D000917:Antibody Formation / Q000235:genetics; D001402:B-Lymphocytes / Q000276:immunology*; D054418:Chemokine CCL20 / Q000378:metabolism; D005786:Gene Expression Regulation; D018858:Germinal Center / Q000166:cytology / Q000276:immunology*; D006241:Haptens; D006433:Hemocyanins / Q000276:immunology; D056747:Immunomodulation; D008213:Lymphocyte Activation; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D054399:Receptors, CCR6 / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: C098951:4-hydroxy-3-nitrophenylacetyl-keyhole limpet hemocyanin; C498126:CCR6 protein, mouse; D054418:Chemokine CCL20; D006241:Haptens; D054399:Receptors, CCR6; D006433:Hemocyanins