title: Partial purification of superoxide dismutase and peroxidase from ber (Zizyphus mauritiana Lamk.) fruit using anion exchange chromatography.
abstract: Partial purification of the two enzymes i.e. superoxide dismutase (SOD) and peroxidase (POX) from ber pulp has been obtained by passing the ammonium sulphate fraction through a diethyl amino ethyl-cellulose (DEAE-Cellulose) column. The fractions showing SOD and POX activity were pooled separately and passed through a Sephadex G100 column for further purification. SOD was purified 12.2 fold with 12.6% yield while POX was purified 15.6 fold with 19.3% yield. Approximate molecular mass for SOD and POX, as judged by gel filtration method was 35.6 and 81.5 kDa, respectively.
authors.affiliation: Department of Molecular Biology & Biotechnology, Rajasthan College of Agriculture, Maharana Pratap University of Agriculture & Technology, Udaipur, 313 001 Rajasthan India.
authors.forename: Sunil
authors.initials: S
authors.lastname: Kumar
authors.forename: Sarla P
authors.initials: SP
authors.lastname: Malhotra
keywords: Ber fruit; DEAE-cellulose chromatography; Zizyphus mauritiana Lamk; fold purification; peroxidase; superoxide dismutase; yield