title: The selective role of ECM components on cell adhesion, morphology, proliferation and communication in vitro.
abstract: Cell binding to the extracellular matrix (ECM) is essential for cell and tissue functions. In this context, each tissue consists of a unique ECM composition, which may be responsible for tissue-specific cell responses. Due to the complexity of ECM-cell interactions-which depend on the interplay of inside-out and outside-in signaling cascades, cell and tissue specificity of ECM-guidance is poorly understood. In this paper, we investigate the role of different ECM components like laminin, fibronectin, and collagen type I with respect to the essential cell behaviour patterns: attachment dynamics such as adhesion kinetic and force, formation of focal adhesion complexes, morphology, proliferation, and intercellular communication. A detailed in vitro comparison of fibroblasts, endothelial cells, osteoblasts, smooth muscle cells, and chondrocytes reveals significant differences in their cell responses to the ECM: cell behaviour follows a cell specific ligand priority ranking, which was independent of the cell type origin. Fibroblasts responded best to fibronectin, chondrocytes best to collagen I, the other cell types best to laminin. This knowledge is essential for optimization of tissue-biomaterial interfaces in all tissue engineering applications and gives insight into tissue-specific cell guidance.
authors.affiliation: Laser Zentrum Hannover e.V., Hollerithallee 8, 30419 Hannover, Germany. s.schlie@lzh.de
authors.forename: Sabrina
authors.initials: S
authors.lastname: Schlie-Wolter
authors.forename: Anaclet
authors.initials: A
authors.lastname: Ngezahayo
authors.forename: Boris N
authors.initials: BN
authors.lastname: Chichkov
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002450:Cell Communication; D002452:Cell Count; D049109:Cell Proliferation; D048430:Cell Shape; D019902:Chondrocytes / Q000378:metabolism; D024042:Collagen Type I / Q000378:metabolism; D042783:Endothelial Cells / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D022001:Focal Adhesions / Q000378:metabolism; D006801:Humans; D007797:Laminin / Q000378:metabolism; D008024:Ligands; D051379:Mice; D032389:Myocytes, Smooth Muscle / Q000378:metabolism; D041681:NIH 3T3 Cells; D010006:Osteoblasts / Q000378:metabolism; D013997:Time Factors
chemical_list: D024042:Collagen Type I; D016326:Extracellular Matrix Proteins; D005353:Fibronectins; D007797:Laminin; D008024:Ligands