title: Evaluation of diminished microbial contamination in handling of a novel daily disposable flat pack contact lens.
abstract: OBJECTIVE
  To evaluate the efficacy of a novel daily disposable (DD) flat package in regard to microbial contamination on the anterior and posterior surfaces of a contact lens (CL) during handling of the lens for insertion.
  
  
  METHODS
  Four kinds of commercially available general blister-packed daily disposable contact lenses (DD CLs) as controls and a novel Magic 1-day Menicon Flat Pack as a test lens were used for this in vitro study. Lenses were removed from their packages using fingers coated with fluorescein 3 to 5 μm beads or an approximately 7×10(2) to 2×10(3) colony-forming unit (CFU)/mL Staphylococcus aureus suspension. The transfer of fluorescein beads to the surface of the lenses was then observed by fluorescence microscopy. Microbial contamination on the lenses was observed by light microscopy after a 2-day incubation period; and, the number of colonies isolated from the contaminated lenses was determined after 4 days of incubation.
  
  
  RESULTS
  The number of fluorescein beads on the Magic lens was significantly less (p<0.05) than that of the general blister-packed control lenses. Adherence of microbial colonies was observed on both inner and outer surfaces of general blister-packed lenses, whereas no colony formation was found on the inner surface of the Magic lens, and the lowest bacterial adherence was observed for the Magic lens.
  
  
  CONCLUSIONS
  The data demonstrated that placement of the Magic DD lens onto the eye is accompanied by diminished microbial contamination compared with general blister-packed DD CLs. Eye care professionals; however, should instruct patients to comply with intended use of DD CLs to prevent CL-associated microbial keratitis. In all cases, hand washing is mandated prelens insertion.
authors.affiliation: Research & Development, Menicon Co., Ltd., Aoi, Naka-ku, Nagoya, Aichi, Japan.
authors.forename: Miya
authors.initials: M
authors.lastname: Nomachi
authors.forename: Kotaro
authors.initials: K
authors.lastname: Sakanishi
authors.forename: Hideji
authors.initials: H
authors.lastname: Ichijima
authors.forename: H Dwight
authors.initials: HD
authors.lastname: Cavanagh
mesh_terms: D001422:Bacterial Adhesion; D015169:Colony Count, Microbial; D003263:Contact Lenses, Hydrophilic / Q000382:microbiology*; D004866:Equipment Contamination; D006235:Hand Disinfection; D013211:Staphylococcus aureus / Q000302:isolation & purification*