title: Feline vector-borne pathogens in the north and centre of Portugal.
abstract: BACKGROUND
  In recent years, several clinical cases and epidemiological studies of feline vector-borne diseases (FVBD) have been reported worldwide. Nonetheless, information on FVBD agents and their prevalence in Portugal is scarce.
  
  
  METHODS
  Three-hundred and twenty domestic cats presented to 30 veterinary medical centres in the north and centre regions of Portugal were randomly sampled. Blood was assayed by real-time polymerase chain reaction (PCR) for genera Anaplasma/Ehrlichia, genus Babesia, Hepatozoon canis, Hepatozoon felis, Leishmania infantum and the genus Rickettsia. Babesia-positive samples were further tested for Babesia canis and Babesia vogeli.
  
  
  RESULTS
  Eighty (25.0%) out of the 320 cats were positive to at least one vector-borne agent, including seven (2.2%) cats co-infected with two agents. Two cats (0.6%) were infected with Anaplasma/Ehrlichia spp., four (1.3%) with B. canis, 26 (8.1%) with B. vogeli, 50 (15.6%) with H. felis, one (0.3%) with L. infantum and four (1.3%) with Rickettsia spp. No cat tested positive for H. canis. One cat (0.3%) was co-infected with B. canis and B. vogeli, three (0.9%) with B. vogeli and H. felis, one (0.3%) with H. felis and L. infantum, and two (0.6%) with H. felis and Rickettsia spp.
  
  
  CONCLUSIONS
  A considerable prevalence of infection with vector-borne pathogens among the domestic feline population of the north and centre of Portugal has been revealed by the present study. Additionally, this is the first detection of B. vogeli in cats from Europe and of H. felis in cats from Portugal.
authors.affiliation: Department of Veterinary Medicine, Escola Universitária Vasco da Gama,Coimbra, Portugal.
authors.forename: Hugo
authors.initials: H
authors.lastname: Vilhena
authors.forename: Verónica L
authors.initials: VL
authors.lastname: Martinez-Díaz
authors.forename: Luís
authors.initials: L
authors.lastname: Cardoso
authors.forename: Lisete
authors.initials: L
authors.lastname: Vieira
authors.forename: Laura
authors.initials: L
authors.lastname: Altet
authors.forename: Olga
authors.initials: O
authors.lastname: Francino
authors.forename: Josep
authors.initials: J
authors.lastname: Pastor
authors.forename: Ana C
authors.initials: AC
authors.lastname: Silvestre-Ferreira
mesh_terms: D000709:Anaplasma / Q000302:isolation & purification; D000818:Animals; D001403:Babesia / Q000302:isolation & purification; D001424:Bacterial Infections / Q000453:epidemiology* / Q000382:microbiology*; D001769:Blood / Q000382:microbiology / Q000469:parasitology; D002371:Cat Diseases / Q000453:epidemiology* / Q000469:parasitology*; D002415:Cats; D003044:Coccidia / Q000302:isolation & purification; D004536:Ehrlichia / Q000302:isolation & purification; D005260:Female; D018314:Leishmania infantum / Q000302:isolation & purification; D008297:Male; D010272:Parasitic Diseases / Q000453:epidemiology* / Q000469:parasitology*; D011174:Portugal / Q000453:epidemiology; D015995:Prevalence; D060888:Real-Time Polymerase Chain Reaction; D012281:Rickettsia / Q000302:isolation & purification