title: Vectorial charge transfer reactions on the donor side of manganese-depleted and reconstituted photosystem 2 core complexes.
abstract: The light-induced functioning of photosystem 2 (PS 2) is directly linked to the translocation of both electrons and protons across the membrane, which results in the formation of transmembrane electric potential difference (ΔΨ). Generation of ΔΨ due to S-state transitions of the water oxidation complex was demonstrated for the first time in Mn-depleted and reconstituted PS 2 core complexes incorporated into liposomes. The kinetics and relative amplitudes of the electrogenic reactions in dark-adapted samples during S1→S2, S2→S3, and S4→S0 transitions in response to the first, second and third laser flashes were comparable to those obtained in the intact PS 2 core particles. These results expand current understanding of the nature and mechanisms of electrogenic (vectorial) reactions due to a charge transfer on the donor side of PS 2.
authors.affiliation: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119234, Russia.
authors.forename: I O
authors.initials: IO
authors.lastname: Petrova
authors.forename: V N
authors.initials: VN
authors.lastname: Kurashov
authors.forename: A A
authors.initials: AA
authors.lastname: Zaspa
authors.forename: A Yu
authors.initials: AY
authors.lastname: Semenov
authors.forename: M D
authors.initials: MD
authors.lastname: Mamedov
mesh_terms: D007700:Kinetics; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D008345:Manganese / Q000172:deficiency* / Q000378:metabolism*; D008564:Membrane Potentials; D045332:Photosystem II Protein Complex / Q000737:chemistry* / Q000378:metabolism*; D018892:Proton-Motive Force; D018724:Spinacia oleracea / Q000201:enzymology
chemical_list: D008081:Liposomes; D045332:Photosystem II Protein Complex; D008345:Manganese