title: Bioconversion of un-pretreated lignocellulosic materials by a microbial consortium XDC-2.
abstract: The present study investigated the degradation of un-pretreated wheat straw, corn stalk, and rice straw by a lignocellulose-degrading microbial consortium XDC-2. Following six days of cultivation, exocellular xylanase activities were 414.9, 491.9, and 335 U/mL, respectively. After 12 days, the rice straw had lost 39.71% of its weight, hemicellulose and cellulose losses of 78.27% and 14.08%, respectively. The total amount of volatile products reached a maximum on day six for rice straw degradation. The four major types of volatile products were acetic acid, propionic acid, butanoic acid, and glycerin, all of which would be suitable substrates for conversion to methanol by anaerobic digestion. According to PCR-DGGE analysis, XDC-2 remained stable during the degradation process of untreated lignocellulosic biomass. These results demonstrate the potential for further development and application of XDC-2; it is capable of degrading un-pretreated lignocellulosic materials, and has a low cost of operation.
authors.affiliation: College of Agronomy and Biotechnology, Center of Biomass Engineering, China Agricultural University, Beijing 100193, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Hui
authors.forename: Li
authors.initials: L
authors.lastname: Jiajia
authors.forename: Lü
authors.initials: L
authors.lastname: Yucai
authors.forename: Guo
authors.initials: G
authors.lastname: Peng
authors.forename: Wang
authors.initials: W
authors.lastname: Xiaofen
authors.forename: Mochidzuki
authors.initials: M
authors.lastname: Kazuhiro
authors.forename: Cui
authors.initials: C
authors.lastname: Zongjun
mesh_terms: D001419:Bacteria / Q000378:metabolism; D001673:Biodegradation, Environmental; D001709:Biotechnology / Q000379:methods*; D058645:Denaturing Gradient Gel Electrophoresis; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism; D005110:Extracellular Space / Q000201:enzymology; D008401:Gas Chromatography-Mass Spectrometry; D006863:Hydrogen-Ion Concentration; D008031:Lignin / Q000378:metabolism*; D059013:Microbial Consortia; D025341:Principal Component Analysis; D012996:Solutions; D013997:Time Factors; D014835:Volatilization; D014866:Waste Products / Q000032:analysis
chemical_list: D012996:Solutions; D014866:Waste Products; C036909:lignocellulose; D008031:Lignin; D043364:Endo-1,4-beta Xylanases