title: A live attenuated strain of Yersinia pestis ΔyscB provides protection against bubonic and pneumonic plagues in mouse model.
abstract: To develop a safe and effective live plague vaccine, the ΔyscB mutant was constructed based on Yersinia pestis biovar Microtus strain 201 that is avirulent to humans, but virulent to mice. The virulence, immunogenicity and protective efficacy of the ΔyscB mutant were evaluated in this study. The results showed that the ΔyscB mutant was severely attenuated, elicited a higher F1-specific antibody titer and provided protective efficacy against bubonic and pneumonic plague in mouse model. The ΔyscB mutant could induce the secretion of both Th1-associated cytokines (IFN-γ, IL-2 and TNF-α) and Th2-associated cytokines (IL-4 and IL-10). Taken together, the ΔyscB mutant represented a potential vaccine candidate based on its ability to generate strong humoral and cell-mediated immune responses and to provide good protection against both subcutaneous and intranasal Y. pestis challenge.
authors.affiliation: Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China.
authors.forename: Xuecan
authors.initials: X
authors.lastname: Zhang
authors.forename: Zhizhen
authors.initials: Z
authors.lastname: Qi
authors.forename: Zongmin
authors.initials: Z
authors.lastname: Du
authors.forename: Yujing
authors.initials: Y
authors.lastname: Bi
authors.forename: Qingwen
authors.initials: Q
authors.lastname: Zhang
authors.forename: Yafang
authors.initials: Y
authors.lastname: Tan
authors.forename: Huiying
authors.initials: H
authors.lastname: Yang
authors.forename: Youquan
authors.initials: Y
authors.lastname: Xin
authors.forename: Ruifu
authors.initials: R
authors.lastname: Yang
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007378:Interleukins; D051379:Mice; D008807:Mice, Inbred BALB C; D018832:Molecular Chaperones / Q000235:genetics / Q000276:immunology; D016254:Mutagenesis, Insertional; D010930:Plague / Q000276:immunology / Q000517:prevention & control*; D010931:Plague Vaccine / Q000276:immunology / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha; D014613:Vaccines, Attenuated / Q000276:immunology / Q000494:pharmacology; D037521:Virulence Factors; D015010:Yersinia pestis / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity
chemical_list: D001426:Bacterial Proteins; D007378:Interleukins; D018832:Molecular Chaperones; D010931:Plague Vaccine; D014409:Tumor Necrosis Factor-alpha; D014613:Vaccines, Attenuated; D037521:Virulence Factors; C117911:YscB protein, Yersinia pestis