title: Bio-composites composed of a solid free-form fabricated polycaprolactone and alginate-releasing bone morphogenic protein and bone formation peptide for bone tissue regeneration.
abstract: Biomedical scaffolds should be designed with highly porous three-dimensional (3D) structures that have mechanical properties similar to the replaced tissue, biocompatible properties, and biodegradability. Here, we propose a new composite composed of solid free-form fabricated polycaprolactone (PCL), bone morphogenic protein (BMP-2) or bone formation peptide (BFP-1), and alginate for bone tissue regeneration. In this study, PCL was used as a mechanical supporting component to enhance the mechanical properties of the final biocomposite and alginate was used as the deterring material to control the release of BMP-2 and BFP-1. A release test revealed that alginate can act as a good release control material. The in vitro biocompatibilities of the composites were examined using osteoblast-like cells (MG63) and the alkaline phosphatase (ALP) activity and calcium deposition were assessed. The in vitro test results revealed that PCL/BFP-1/Alginate had significantly higher ALP activity and calcium deposition than the PCL/BMP-2/Alginate composite. Based on these findings, release-controlled BFP-1 could be a good growth factor for enhancement of bone tissue growth and the simple-alginate coating method will be a useful tool for fabrication of highly functional biomaterials through release-control supplementation.
authors.affiliation: Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, South Korea.
authors.forename: MinSung
authors.initials: M
authors.lastname: Kim
authors.forename: Won-Kyo
authors.initials: WK
authors.lastname: Jung
authors.forename: GeunHyung
authors.initials: G
authors.lastname: Kim
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000595:Amino Acid Sequence; D001672:Biocompatible Materials; D019485:Bone Morphogenetic Proteins / Q000737:chemistry*; D001842:Bone and Bones / Q000502:physiology*; D002460:Cell Line; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D010006:Osteoblasts / Q000166:cytology; D011091:Polyesters / Q000737:chemistry*; D012038:Regeneration
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D019485:Bone Morphogenetic Proteins; D006603:Hexuronic Acids; D011091:Polyesters; C016240:polycaprolactone; D020723:Glucuronic Acid