title: TLC bioautography: high throughput technique for screening of bioactive natural products.
abstract: TLC bioautography is a method that combines chromatographic separation and in situ biological activity determination. The antimicrobial, antioxidant and enzyme inhibitory activities can be performed on TLC bioautography. This method is mainly used for preliminary screening natural products possessing these biological activities and for the bioactivity-directed fractionation and isolation of active components from complex extracts. This review covers the mechanisms and methodology of the antimicrobial, antioxidant activities, and enzyme inhibition adopted on TLC. It will be in particular discuss the recent advances in these technologies and assess their applications in targeting natural products.
authors.affiliation: University of Traditional Chinese Medicine, 1200 Cailun Road, Institute of Chinese Materia Medica, Shanghai 201203, China. laurawu2000@hotmail.com.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Cheng
authors.forename: Tao
authors.initials: T
authors.lastname: Wu
mesh_terms: D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology; D001688:Biological Products / Q000032:analysis* / Q000494:pharmacology; D002855:Chromatography, Thin Layer / Q000379:methods*; D057166:High-Throughput Screening Assays
chemical_list: D000975:Antioxidants; D001688:Biological Products