title: Effectiveness of strict anti-contamination criteria for non-invasive foetal sex determination in a clinical setting.
abstract: OBJECTIVE
  The aim of this study is to prevent false positive results in non-invasive foetal sex determination from pregnant women's plasma following strict anti-contamination criteria.
  
  
  METHODS
  cffDNA from 200 pregnant women (mean gestational age of 8.3 ± 0.2 weeks) was analyzed based on genetic forensic anti-contamination procedures and classical non-invasive foetal sex determination techniques.
  
  
  RESULTS
  No false positive or false negative was reported. The sensitivity and the specificity reached both a 100% value.
  
  
  CONCLUSIONS
  Prevention of contamination following our anti-contamination criteria is a good practice for certain non-invasive sex determination, using cffDNA.
authors.affiliation: Andros Day Surgery, Molecular Biology Unit , Palermo , Italy and.
authors.forename: Alicia
authors.initials: A
authors.lastname: González-Liñán
authors.forename: Lucio
authors.initials: L
authors.lastname: Trizzino
authors.forename: Daniela
authors.initials: D
authors.lastname: Giambelluca
authors.forename: Angelo
authors.initials: A
authors.lastname: Marino
authors.forename: Adolfo
authors.initials: A
authors.lastname: Allegra
mesh_terms: D001774:Blood Chemical Analysis / Q000592:standards; D059018:DNA Contamination; D005189:False Positive Reactions; D005260:Female; D005333:Fetus / Q000166:cytology / Q000378:metabolism; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards; D017410:Practice Guidelines as Topic; D011247:Pregnancy; D011261:Pregnancy Trimester, First / Q000097:blood; D011296:Prenatal Diagnosis / Q000379:methods / Q000592:standards*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012732:Sex Determination Analysis / Q000379:methods* / Q000592:standards*; D051876:Sex-Determining Region Y Protein / Q000235:genetics
chemical_list: C497745:SRY protein, human; D051876:Sex-Determining Region Y Protein