title: Antidiabetic and in vitro antioxidant potential of Hybanthus enneaspermus (Linn) F. Muell in streptozotocin-induced diabetic rats.
abstract: OBJECTIVE
  To evaluate antidiabetic and antioxidant potential of Hybanthus enneaspermus in different models.
  
  
  METHODS
  The oral glucose tolerance test (OGTT) and normoglycemic effect of alcoholic extract of Hybanthus enneaspermus (AHE) were evaluated at a dose of 125, 250 and 500 mg/kg p.o. while hypoglycemic activity and effect on body weight were tested at 250 and 500 mg/kg p.o. per day for 21 days in Streptozotocin (STZ) induced diabetic rats. Further, glucose uptake by hemidiaphram was also evaluated. The total polyphenolic and flavonoid were determined and their correlation with various antioxidant assays was also determined.
  
  
  RESULTS
  The results showed high level of phenolic content in AHE. AHE also exhibited higher total antioxidant capacity, good reducing power and a significant scavenger of reactive oxygen species like DPPH radical, nitric oxide, hydrogen peroxide and deoxyribose. Furthermore there was a significant increase in the body weight and decrease in the blood glucose level on treatment with the AHE. AHE increased glucose uptake on isolated rat hemi-diaphragm compared to control group.
  
  
  CONCLUSIONS
  AHE reduce blood glucose level in STZ-induced diabetic model. It does not show significant effect in normoglycemic study but showes significant effect in OGT. AHE has significant antioxidant activity, which may be attributed to high phenolic content.
authors.affiliation: Pharmacognosy Research Laboratory, Department of Pharmaceutics, Institute of Technology, Banaras Hindu University, Varanasi-221005, India.
authors.forename: D K
authors.initials: DK
authors.lastname: Patel
authors.forename: R
authors.initials: R
authors.lastname: Kumar
authors.forename: S K
authors.initials: SK
authors.lastname: Prasad
authors.forename: K
authors.initials: K
authors.lastname: Sairam
authors.forename: S
authors.initials: S
authors.lastname: Hemalatha
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000627:therapeutic use*; D001835:Body Weight; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D005951:Glucose Tolerance Test; D007004:Hypoglycemic Agents / Q000302:isolation & purification / Q000627:therapeutic use*; D008297:Male; D010936:Plant Extracts / Q000302:isolation & purification / Q000627:therapeutic use*; D017208:Rats, Wistar; D029649:Violaceae / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D007004:Hypoglycemic Agents; D010936:Plant Extracts
keywords: Antioxidant; Diabetic; Flavonoid; Flavonol; Glucose uptake; Hybanthus enneaspermus; Total phenol