title: Lectin-like oxidized LDL receptor-1 is palmitoylated and internalizes ligands via caveolae/raft-dependent endocytosis.
abstract: Lectin-like oxidized low-density lipoprotein (LDL) receptor-1 (LOX-1) is an endothelial scavenger receptor that is important for oxidized low-density lipoprotein uptake. LOX-1 functions as an oligomer; however, little is known about the oligomeric complex and ligand processing after recognition by LOX-1. Here, we found that LOX-1 recognized and internalized ligands through the caveolae/raft-dependent endocytosis pathway in human coronary artery endothelial cells. Furthermore, we demonstrated that LOX-1 was palmitoylated and that both cysteine 36 and cysteine 46 were necessary for the recruitment of LOX-1 into raft microdomains and for its ligand uptake ability.
authors.affiliation: National Food Research Institute, NARO, 2-1-12, Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
authors.forename: Miyuki
authors.initials: M
authors.lastname: Kumano-Kuramochi
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: Xie
authors.forename: Shoko
authors.initials: S
authors.lastname: Kajiwara
authors.forename: Shiro
authors.initials: S
authors.lastname: Komba
authors.forename: Takashi
authors.initials: T
authors.lastname: Minowa
authors.forename: Sachiko
authors.initials: S
authors.lastname: Machida
mesh_terms: D021941:Caveolae / Q000378:metabolism*; D002478:Cells, Cultured; D004705:Endocytosis / Q000187:drug effects; D019833:Genistein / Q000494:pharmacology; D006801:Humans; D008024:Ligands; D054878:Lipoylation; D009761:Nystatin / Q000494:pharmacology; D051127:Scavenger Receptors, Class E / Q000378:metabolism*
chemical_list: D008024:Ligands; C495137:OLR1 protein, human; D051127:Scavenger Receptors, Class E; D009761:Nystatin; D019833:Genistein