title: Canine cutaneous histiocytomas are clonal lesions as defined by X-linked clonality testing.
abstract: Canine cutaneous histiocytoma (CCH) is the most common skin tumour of young dogs. CCH is assumed to be a tumour, but as these lesions often undergo complete regression, they have also been proposed to represent hamartomas or unregulated hyperplasia of polyclonal histiocytic cells. To demonstrate a monoclonal origin of CCH, and thereby a probable neoplastic nature, a clonality assay was established based on the heterozygosity of a polymorphic microsatellite within the canine androgen receptor located on the X chromosome. Analysis of 11 histiocytomas identified a non-random X chromosome inactivation of one androgen receptor allele. CCH may, therefore, be a clonal lesion and of neoplastic nature.
authors.affiliation: Department of Veterinary Pathology, Freie Universität Berlin, Robert-von-Ostertag-Str 15, 14163 Berlin, Germany.
authors.forename: N M
authors.initials: NM
authors.lastname: Delcour
authors.forename: R
authors.initials: R
authors.lastname: Klopfleisch
authors.forename: A D
authors.initials: AD
authors.lastname: Gruber
authors.forename: A Th A
authors.initials: AT
authors.lastname: Weiss
mesh_terms: D000818:Animals; D002999:Clone Cells; D004283:Dog Diseases / Q000235:genetics*; D004285:Dogs; D018219:Histiocytoma, Benign Fibrous / Q000235:genetics / Q000662:veterinary*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012878:Skin Neoplasms / Q000235:genetics / Q000662:veterinary*; D018911:Trinucleotide Repeats; D049951:X Chromosome Inactivation
keywords: X chromosome; clonality assay; dog; histiocytoma