title: A novel marker for the species-specific detection and quantitation of Vibrio cholerae by targeting an outer membrane lipoprotein lolB gene.
abstract: Vibrio cholerae O1 and O139 are the major serotypes associated with illness, and some V. cholera non-O1 and non-O139 isolates produce cholera toxin. The present study describes a quantitative polymerase chain reaction (qPCR) assay for the species-specific detection and quantitation of V. cholera using a primer pair based on an outer membrane lipoprotein lolB gene for the amplification of a 195 bp DNA fragment. The qPCR primer set for the accurate diagnosis of V. cholera was developed from publically available genome sequences. This quantitative PCR-based method will potentially simplify and facilitate the diagnosis of this pathogen and guide disease management.
authors.affiliation: National Academy of Agricultural Science, Rural Development Administration, Suwon, 441-707, Korea.
authors.forename: Min Seok
authors.initials: MS
authors.lastname: Cho
authors.forename: Tae-Young
authors.initials: TY
authors.lastname: Ahn
authors.forename: Kiseong
authors.initials: K
authors.lastname: Joh
authors.forename: Soon-Young
authors.initials: SY
authors.lastname: Paik
authors.forename: Oh-Sang
authors.initials: OS
authors.lastname: Kwon
authors.forename: Won-Hwa
authors.initials: WH
authors.lastname: Jheong
authors.forename: Yochan
authors.initials: Y
authors.lastname: Joung
authors.forename: Dong Suk
authors.initials: DS
authors.lastname: Park
mesh_terms: D058491:Bacterial Load / Q000379:methods*; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics*; D002771:Cholera / Q000175:diagnosis / Q000382:microbiology; D017931:DNA Primers / Q000235:genetics; D008074:Lipoproteins / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D041241:Vibrio cholerae O1 / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D017931:DNA Primers; D008074:Lipoproteins