title: Absence of NF-κB subunit p50 ameliorates cold immobilization stress-induced gastric ulcers.
abstract: Stress ulcers are a common complication in critically ill patients, but the underlying mechanism is little known. This study characterized the function of the p50 subunit of NF-κB in an experimental model of cold immobilization stress-induced gastric ulcers. Stress-induced gastric mucosal inflammation and gastric injury were examined in wild-type and NF-κB p50-deficient mice. When subjected to cold immobilization stress, NF-κB was rapidly activated in the gastric mucosa in WT mice whereas the majority of κB DNA-binding activity was abrogated from p50(-/-) mice. Deficiency of p50 ameliorated stress-induced expression of TNF-α, MIP-2, and ICAM-1, resulting in reduced mucosal accumulation of neutrophils and gastric injury. These data indicated a critical role for the p50 in the gastric mucosal inflammatory response to cold restraint stress.
authors.affiliation: Department of Orthopaedics, Riyuexing Geriatric Hospital, Shanghai 200090, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Ye
authors.forename: Pan-Yu
authors.initials: PY
authors.lastname: Zhou
authors.forename: Meng
authors.initials: M
authors.lastname: Jia
authors.forename: Xiao-Song
authors.initials: XS
authors.lastname: Cheng
authors.forename: Yi-Tao
authors.initials: YT
authors.lastname: Jia
authors.forename: Shuo-Gui
authors.initials: SG
authors.lastname: Xu
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D003080:Cold Temperature; D024202:Electrophoretic Mobility Shift Assay; D007103:Immobilization; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D052002:NF-kappa B p50 Subunit / Q000378:metabolism*; D009195:Peroxidase / Q000378:metabolism; D013276:Stomach Ulcer / Q000517:prevention & control*; D013312:Stress, Physiological
chemical_list: D052002:NF-kappa B p50 Subunit; D009195:Peroxidase