title: The influence of Bauhinia forficata Link subsp. pruinosa tea on lipid peroxidation and non-protein SH groups in human erythrocytes exposed to high glucose concentrations.
abstract: BACKGROUND
  Bauhinia forficata (BF) has been traditionally used as tea in folk medicine of Brazil for treatment of Diabetes mellitus (DM).
  
  
  OBJECTIVE
  To evaluate the effects of BF leaf tea on markers of oxidative damage and antioxidant levels in an experimental model of hyperglycemia in human erythrocytes in vitro.
  
  
  METHODS
  Human erythrocytes were incubated with high glucose concentrations or glucose and BF tea for 24h and 48h. After incubation lipid peroxidation and non-protein SH levels were analyzed. Moreover, quantification of polyphenols and flavonoids, iron chelating property, scavenging of DPPH, and prevention of lipid peroxidation in isolated lipids were also assessed.
  
  
  RESULTS
  A significant amount of polyphenols and flavonoids was observed. The main components found by LC-MS analysis were quercetin-3-O-(2-rhamnosyl) rutinoside, kaempferol-3-O-(2-rhamnosyl) rutinoside, quercetin-3-O-rutinoside and kaempferol-3-O-rutinoside. BF tea presents important antioxidant and chelating properties. Moreover, BF tea was effective to increase non-protein SH levels and reduce lipid peroxidation induced by high glucose concentrations in human erythrocytes.
  
  
  CONCLUSIONS
  The antioxidant effects of BF tea could be related to the presence of different phenolic and flavonoids components. We believe that these components can be responsible to protect human erythrocytes exposed to high glucose concentrations against oxidative damage.
authors.affiliation: Universidade Federal do Pampa, UNIPAMPA, Campus Uruguaiana, Uruguaiana, RS, Brazil.
authors.forename: Andréia C F
authors.initials: AC
authors.lastname: Salgueiro
authors.forename: Carina Q
authors.initials: CQ
authors.lastname: Leal
authors.forename: Matheus C
authors.initials: MC
authors.lastname: Bianchini
authors.forename: Ianeli O
authors.initials: IO
authors.lastname: Prado
authors.forename: Andreas S L
authors.initials: AS
authors.lastname: Mendez
authors.forename: Robson L
authors.initials: RL
authors.lastname: Puntel
authors.forename: Vanderlei
authors.initials: V
authors.lastname: Folmer
authors.forename: Félix A
authors.initials: FA
authors.lastname: Soares
authors.forename: Daiana S
authors.initials: DS
authors.lastname: Avila
authors.forename: Gustavo O
authors.initials: GO
authors.lastname: Puntel
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D036481:Bauhinia; D001628:Beverages / Q000032:analysis; D002478:Cells, Cultured; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D005419:Flavonoids / Q000032:analysis / Q000494:pharmacology; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D010636:Phenols / Q000032:analysis / Q000494:pharmacology; D018515:Plant Leaves; D013438:Sulfhydryl Compounds / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D013438:Sulfhydryl Compounds; D017392:Thiobarbituric Acid Reactive Substances; D005947:Glucose