title: [Clinical and cytogenetic study of 6 cases of hematological disorders associated with 20q- and t (20;21) (q11;q11) abnormalities].
abstract: OBJECTIVE
  To analyze clinical and cytogenetic features of hematological disorders associated with 20q- and t (20;21) (q11;q11) abnormalities.
  
  
  METHODS
  Following short-term culture of bone marrow cells, karyotypic analysis was carried out with R-banding. 20q- and t(20;21) (q11;q11) was detected by fluorescence in situ hybridization (FISH) using dual-color 20q11/12 probe, ST 20qter /ST 21qter probes, SE20(D20Z1)/SE 13/21 probes, and WC20/WC21 probes.
  
  
  RESULTS
  Six (2.3%) of the 257 patients with 20q- detected by conventional karyotypic analysis were found to have t(20;21) (q11;q11) abnormality. Five cases had myelodysplastic syndrome, 1 had acute lymphoblastic leukemia. Above results were all confirmed by FISH.
  
  
  CONCLUSIONS
  i (20q-), t(20;21) (q11;q11) seems to be a rare but recurrent chromosomal abnormality which is specifically associated with myeloid disease, late occurrence and poor prognosis. The translocation between chromosome 20q11 and 21q11 may form a novel fusion gene which has an important role in the pathogenesis of the disease.
authors.affiliation: Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis, The First Affiliated Hospital of Soochow University, Suzhou, P.R. China.
authors.forename: Chun-xiao
authors.initials: CX
authors.lastname: Wu
authors.forename: Jin-lan
authors.initials: JL
authors.lastname: Pan
authors.forename: Hui-ying
authors.initials: HY
authors.lastname: Qiu
authors.forename: Yong-quan
authors.initials: YQ
authors.lastname: Xue
authors.forename: Su-ning
authors.initials: SN
authors.lastname: Chen
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Ya-fang
authors.initials: YF
authors.lastname: Wu
authors.forename: Juan
authors.initials: J
authors.lastname: Shen
authors.forename: Shu-xiao
authors.initials: SX
authors.lastname: Bai
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000368:Aged; D002872:Chromosome Deletion; D002890:Chromosomes, Human, Pair 20; D002891:Chromosomes, Human, Pair 21; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D009190:Myelodysplastic Syndromes / Q000235:genetics*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*; D014178:Translocation, Genetic