title: How can plant virus satellite RNAs alter the effects of plant virus infection? A study of the changes in the Nicotiana benthamiana proteome after infection by peanut stunt virus in the presence or absence of its satellite RNA.
abstract: Peanut stunt virus (PSV), which belongs to the Cucumovirus genus, is a pathogen of legumes. Certain PSV strains associated with a satellite RNA (satRNA) modify the symptoms of infected plants and interfere with plant metabolism. We used PSV-P genomic transcripts (GTs) with and without PSV-P satRNA and a comparative proteomic 2D-DIGE/MS study to assess their effects on Nicotiana benthamiana infection. When the proteomes of the PSV-P genomic transcripts-infected (no satRNA present) and mock-inoculated plants were compared 29 differentially regulated proteins were found. When comparisons were made for plants infected with PSV-P-GT in the presence or absence of satRNA, and for mock-infected plants and those infected with the satRNA-associated PSV-P-GT, 40 and 60 such proteins, respectively, were found. The presence of satRNA mostly decreased the amounts of the affected host proteins. Proteins involved in photosynthesis and carbohydrate metabolism, for example ferredoxin-NADP-reductase and malate dehydrogenase, are among the identified affected proteins in all comparisons. Proteins involved in protein synthesis and degradation were also affected. Such proteins include chaperonin 60β--whose abundance of the proteins changed for all comparisons--and aminopeptidase that is a satRNA- or PSV-P-GT/satRNA-responsive protein. Additionally, the levels of the stress-related proteins superoxide dismutase and acidic endochitinase Q increased in the PSV-P-GT- and PSV-P-GT/satRNA-infected plants. This study appears to be the first report on plant proteome changes in response to a satRNA presence during viral infection and, as such, may provide a reference for future studies concerning the influence of satRNAs during viral infections.
authors.affiliation: Institute of Plant Protection, National Research Institute, Poznań, Poland. ao.steplowska@iorpib.poznan.pl
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Obrępalska-Stęplowska
authors.forename: Przemysław
authors.initials: P
authors.lastname: Wieczorek
authors.forename: Marta
authors.initials: M
authors.lastname: Budziszewska
authors.forename: Arnika
authors.initials: A
authors.lastname: Jeszke
authors.forename: Jenny
authors.initials: J
authors.lastname: Renaut
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D054884:Host-Pathogen Interactions; D010935:Plant Diseases / Q000235:genetics / Q000821:virology*; D010940:Plant Proteins / Q000378:metabolism; D010942:Plant Viruses / Q000235:genetics* / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods; D019244:RNA, Satellite / Q000235:genetics / Q000378:metabolism*; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism*; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism* / Q000821:virology*
chemical_list: D010940:Plant Proteins; D020543:Proteome; D019244:RNA, Satellite; D012367:RNA, Viral
keywords: 2-DIGE; PSV; Plant proteomics; Proteomic profiling; satRNA