title: BAX inhibitor-1 silencing suppresses white spot syndrome virus replication in red swamp crayfish, Procambarus clarkii.
abstract: BAX inhibitor-1 (BI-1) was originally described as an anti-apoptotic protein in both animal and plant cells. BI-1 overexpression suppresses ER stress-induced apoptosis in animal cells. Inhibition of BI-1 activity could induce the cell death in mammals and plants. However, the function of BI-1 in crustacean immunity was unclear. In this paper, the full-length cDNA of a BI-1 protein in red swamp crayfish, Procambarus clarkii (PcBI-1) was cloned and its expression profiles in normal and infected crayfish were analyzed. The results showed that PcBI-1 was expressed in hemocytes, heart, hepatopancreas, gills, stomach, and intestines of the crayfish and was upregulated after challenged with Vibrio anguillarum and with white spot syndrome virus (WSSV). To determine the function of PcBI-1 in the innate immunity of the crayfish, the RNA interference against PcBI-1 was performed and the results indicated the hemocyte programmed cell death rate was increased significantly and WSSV replication was declined after PcBI-1 knocked down. Altogether, PcBI-1 plays an anti-apoptotic role, wherein high PcBI-1 expression suppresses programmed cell death, which is beneficial for WSSW replication in crayfish.
authors.affiliation: The Key Laboratory of Plant Cell Engineering and Germplasm Innovation of Ministry of Education/Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Jinan, Shandong 250100, China.
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Du
authors.forename: Jiang-Feng
authors.initials: JF
authors.lastname: Lan
authors.forename: Yu-Ding
authors.initials: YD
authors.lastname: Weng
authors.forename: Xiao-Fan
authors.initials: XF
authors.lastname: Zhao
authors.forename: Jin-Xing
authors.initials: JX
authors.lastname: Wang
mesh_terms: D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000235:genetics* / Q000378:metabolism; D060829:Arthropod Proteins / Q000235:genetics* / Q000378:metabolism; D003400:Astacoidea / Q000235:genetics* / Q000276:immunology / Q000382:microbiology* / Q000821:virology; D016923:Cell Death; D003001:Cloning, Molecular; D018076:DNA, Complementary; D005786:Gene Expression Regulation; D009928:Organ Specificity; D034622:RNA Interference; D014733:Vibrio / Q000502:physiology; D014779:Virus Replication; D046848:White spot syndrome virus 1 / Q000502:physiology
chemical_list: D051017:Apoptosis Regulatory Proteins; D060829:Arthropod Proteins; D018076:DNA, Complementary