title: Differences in collagen distribution of healthy and regenerated periodontium. Histomorphometric study in dogs.
abstract: Previous studies have shown that there is a relationship between periodontal disease and the distribution of collagen fibers. This study evaluated the distribution of collagen types I and III in regenerated bone and periodontal ligament, comparing them to the tissues near the regenerated area and to the healthy periodontium. In the third (P3) and fourth (P4) mandibular premolars of 5 healthy mongrel dogs, bilaterally, buccal class 2 furcation lesions were surgically created and chronified for 3 weeks. After that, full flaps were elevated and expanded polytetrafluoroethylene (e-PTFE) membranes were adapted, sutured and recovered by the flaps. Two weeks after surgery, two membranes on the same side were removed and the other membranes were removed four weeks after surgery. The dogs were euthanized at 12 weeks following placement of the e-PTFE membranes. P3 and P4 teeth as well as the second premolars (healthy control teeth) and their periodontal tissues were removed and histologically processed for Collagen Quantification (COLQ). The amount of type III collagen was higher in native bone compared to the regenerated area. For periodontal ligament, COLQ for type I collagen showed statistically significant differences (Tukeys's Multiple Comparison, p⟨0.05) between the regenerated groups and the control group. These differences were not found for type III COLQ. There are significant differences in collagen distribution among the regenerated, native and control tissues. Membrane removal 2 or 4 weeks postoperatively did not influence the collagen composition.
authors.affiliation: Department of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry at Ribeirao Preto, University of Sāo Paulo, Brazil. scombati@forp.usp.br.
authors.forename: Sérgio L S
authors.initials: SL
authors.lastname: Souza
authors.forename: Guilherme O
authors.initials: GO
authors.lastname: Macedo
authors.forename: Adriana M M
authors.initials: AM
authors.lastname: Silveira E Souza
authors.forename: Mário
authors.initials: M
authors.lastname: Taba
authors.forename: Arthur B
authors.initials: AB
authors.lastname: Novaes
authors.forename: Constance
authors.initials: C
authors.lastname: Oliver
authors.forename: Maria C
authors.initials: MC
authors.lastname: Jamur
authors.forename: Vani M A
authors.initials: VM
authors.lastname: Correa
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D001861:Bone Regeneration; D001842:Bone and Bones / Q000502:physiology*; D024042:Collagen Type I / Q000737:chemistry*; D024061:Collagen Type III / Q000737:chemistry*; D004396:Coloring Agents / Q000737:chemistry; D003238:Connective Tissue / Q000473:pathology; D004285:Dogs; D005260:Female; D008297:Male; D008853:Microscopy; D008963:Molar / Q000502:physiology; D010513:Periodontal Ligament / Q000473:pathology / Q000502:physiology; D010519:Periodontium / Q000502:physiology*; D011138:Polytetrafluoroethylene / Q000737:chemistry; D012038:Regeneration; D012737:Sex Factors
chemical_list: D001672:Biocompatible Materials; D024042:Collagen Type I; D024061:Collagen Type III; D004396:Coloring Agents; D011138:Polytetrafluoroethylene