title: First case of Tritirachium oryzae as agent of onychomycosis and its susceptibility to antifungal drugs.
abstract: The first case of Tritirachium oryzae isolated from an Iranian patient is reported. A 44-year-old woman with a lesion in her fingernail was examined for onychomycosis. Direct microscopic examination of the nail clippings revealed fungal filaments and inoculation of portions of the nail clippings on cultures media yielded T. oryzae after 8 days. The isolate was identified as Tritirachium spp. on the basis of gross morphological characteristics of the fungal colony and microscopic characterization of slide cultures. The diagnosis of T. oryzae was confirmed by PCR sequencing of the internal transcribed spacer domain of the rDNA gene. In vitro antifungal susceptibility test demonstrated that the fungus was susceptible to itraconazole and posaconazole. The patient was treated with oral itraconazole.
authors.affiliation: Department of Medical Mycology and Parasitology, Imam Reza Hospital, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. naseria47@yahoo.com
authors.forename: Ali
authors.initials: A
authors.lastname: Naseri
authors.forename: Abdolmajid
authors.initials: A
authors.lastname: Fata
authors.forename: Mohammad Javad
authors.initials: MJ
authors.lastname: Najafzadeh
mesh_terms: D000328:Adult; D000935:Antifungal Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001487:Basidiomycota / Q000187:drug effects* / Q000302:isolation & purification*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D005260:Female; D006801:Humans; D007492:Iran; D017964:Itraconazole / Q000494:pharmacology / Q000627:therapeutic use; D008826:Microbial Sensitivity Tests; D008828:Microbiological Techniques; D008853:Microscopy; D008969:Molecular Sequence Data; D009262:Nails / Q000382:microbiology; D014009:Onychomycosis / Q000175:diagnosis* / Q000382:microbiology* / Q000473:pathology; D017422:Sequence Analysis, DNA; D014230:Triazoles / Q000494:pharmacology
chemical_list: D000935:Antifungal Agents; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D014230:Triazoles; D017964:Itraconazole; C101425:posaconazole