title: Putative protein partners for the human CPI-17 protein revealed by bacterial two-hybrid screening.
abstract: We have previously demonstrated that PKC-potentiated inhibitory protein of protein phosphatase-1 (CPI-17) is expressed in lung endothelium. CPI-17, a specific inhibitor of myosin light chain phosphatase (MLCP), is involved in the endothelial cytoskeletal and barrier regulation. In this paper, we report the identification of fourteen putative CPI-17 interacting proteins in the lung using BacterioMatch Two-Hybrid System. Five of them: plectin 1 isoform 1, alpha II spectrin, OK/SW-CL.16, gelsolin isoform a, and junction plakoglobin are involved in actin cytoskeleton organization and cell adhesion, suggesting possible significance of these binding partners in CPI-17-mediated cytoskeletal reorganization of endothelial cells. Furthermore, we confirmed the specific interaction between plakoglobin and CPI-17, which is affected by the phosphorylation status of CPI-17 in human lung microvascular endothelial cells.
authors.affiliation: Vascular Biology Center, Georgia Health Sciences University, Augusta, GA, USA.
authors.forename: Kyung-mi
authors.initials: KM
authors.lastname: Kim
authors.forename: Djanybek M
authors.initials: DM
authors.lastname: Adyshev
authors.forename: Anita
authors.initials: A
authors.lastname: Kása
authors.forename: Evgeny A
authors.initials: EA
authors.lastname: Zemskov
authors.forename: Irina A
authors.initials: IA
authors.lastname: Kolosova
authors.forename: Csilla
authors.initials: C
authors.lastname: Csortos
authors.forename: Alexander D
authors.initials: AD
authors.lastname: Verin
mesh_terms: D000199:Actins / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism; D004727:Endothelium / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006801:Humans; D047468:Immunoprecipitation; D047908:Intracellular Signaling Peptides and Proteins; D008168:Lung / Q000098:blood supply; D008833:Microcirculation; D008856:Microscopy, Fluorescence; D009124:Muscle Proteins; D043263:Myosin-Light-Chain Phosphatase / Q000378:metabolism; D010749:Phosphoprotein Phosphatases / Q000378:metabolism*; D010766:Phosphorylation; D011485:Protein Binding; D025941:Protein Interaction Mapping; D015398:Signal Transduction; D020798:Two-Hybrid System Techniques; D051185:gamma Catenin / Q000378:metabolism
chemical_list: D000199:Actins; D047908:Intracellular Signaling Peptides and Proteins; D009124:Muscle Proteins; C107507:PPP1R14A protein, human; D051185:gamma Catenin; D010749:Phosphoprotein Phosphatases; D043263:Myosin-Light-Chain Phosphatase