title: Transduction of murine embryonic stem cells by magnetic nanoparticle-assisted lentiviral gene transfer.
abstract: Genetic modification of embryonic stem (ES) cells is a valuable technique when combined with cell replacement strategies. Obtaining stable transgene expression and low-cytotoxicity lentiviral transduction of ES cells is advantageous. It has been shown that the efficiency of transfection and transduction approaches can be increased by magnetic nanoparticles (MNPs). Here, we present a protocol for MNP-assisted lentiviral transduction of adherent mouse ES cells. The application of MNPs increased transduction efficiency and provided the opportunity of cell positioning by a magnetic field.
authors.affiliation: Institute of Pharmacology and Toxicology, University of Bonn, Bonn, Germany.
authors.forename: Sarah
authors.initials: S
authors.lastname: Rieck
authors.forename: Katrin
authors.initials: K
authors.lastname: Zimmermann
authors.forename: Daniela
authors.initials: D
authors.lastname: Wenzel
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D053595:Embryonic Stem Cells / Q000378:metabolism*; D005434:Flow Cytometry; D015870:Gene Expression; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000235:genetics*; D016086:Lentivirus / Q000235:genetics*; D058185:Magnetite Nanoparticles; D051379:Mice; D008856:Microscopy, Fluorescence; D014161:Transduction, Genetic
chemical_list: D058185:Magnetite Nanoparticles