title: Visualization of implanted GL261 glioma cells in living mouse brain slices using fluorescent 4-(4-(dimethylamino)-styryl)-N-methylpyridinium iodide (ASP+).
abstract: Here we describe a new method of glioma cell visualization in living brain slices that can be used for evaluation of tumor size or visualization of internal tumor structures. Glial cells, as well as glioma cells of glial origin, express high levels of organic cation transporters. We demonstrate that application of a fluorescent substrate for these transporters 4-(4-(dimethylamino)-styryl)-N-methylpyridinium iodide (ASP+) to the incubation medium leads to quick accumulation of fluorescence in glioma cells during early developmental stages and in astrocytes, but not in neurons. Stained brain slices can be immediately investigated using confocal or fluorescence microscopy. Glioma and glial cells can be discriminated from each other because of their different morphology. The method described has the advantage of staining living tissue and is simple to perform.
authors.affiliation: Department of Biochemistry, Universidad Central del Caribe, School of Medicine, Bayamón, Puerto Rico.
authors.forename: Lilia Y
authors.initials: LY
authors.lastname: Kucheryavykh
authors.forename: Yuriy V
authors.initials: YV
authors.lastname: Kucheryavykh
authors.forename: Kimberleve
authors.initials: K
authors.lastname: Rolón-Reyes
authors.forename: Serguei N
authors.initials: SN
authors.lastname: Skatchkov
authors.forename: Misty J
authors.initials: MJ
authors.lastname: Eaton
authors.forename: Luis A
authors.initials: LA
authors.lastname: Cubano
authors.forename: Mikhail
authors.initials: M
authors.lastname: Inyushin
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism / Q000473:pathology; D001932:Brain Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D027682:Cation Transport Proteins / Q000378:metabolism; D005910:Glioma / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D011726:Pyridinium Compounds; D013194:Staining and Labeling / Q000379:methods*
chemical_list: C032315:4-(4-dimethylaminostyryl)-1-methylpyridinium; D027682:Cation Transport Proteins; D011726:Pyridinium Compounds