title: Expression of N-acetylglucosaminyltransferase V in the subserosal layer correlates with postsurgical survival of pathological tumor stage 2 carcinoma of the gallbladder.
abstract: BACKGROUND
  N-Acetylglucosaminyltransferase V (GnT-V), an enzyme that catalyzes the β1-6 branching of N-acetylglucosamine on asparagine-linked oligosaccharides of cellular proteins, enhances the malignant behaviors of carcinoma cells in experimental models. The aim of this study was to determine clinical significance of GnT-V expression in human pT2 gallbladder carcinoma with simple in vitro experiments.
  
  
  METHODS
  Ninety patients with pT2 gallbladder carcinoma were included for this study. The in vitro and in vivo biological effects of GnT-V were investigated using gallbladder carcinoma cells with variable GnT-V expression levels induced by a small interfering RNA.
  
  
  RESULTS
  Of the 90 cases, 57 showed positive staining and the remaining 33 demonstrated negative staining, the subcellular localization in the 57 cases was classified into the granular-type in 31 cases and the diffuse-type in 26 cases. In 76 cases with curative resection, postsurgical survival was significantly poorer in those showing positive staining than in those showing negative staining (P = 0.028). In all of the 76 cases, postsurgical recurrence was significantly more frequent in those showing diffuse-type localization than in those showing negative staining. Experimental analyses demonstrated that the down-regulation of GnT-V expression in gallbladder carcinoma cells induced suppression of cell growth in vitro. The expression levels of GnT-V in the cells were highly correlated with the rapid in vivo growth coupled with the enhanced angiogenesis, and the tendency to form liver metastasis.
  
  
  CONCLUSIONS
  GnT-V expression in the subserosal layer of pT2 gallbladder carcinoma is correlated with the aggressiveness of the disease.
authors.affiliation: Department of Surgery, Institute of Gastroenterology, Tokyo Women's Medical University, Shinjuku-ku, Tokyo, Japan.
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Onuki
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Sugiyama
authors.forename: Kazunori
authors.initials: K
authors.lastname: Ishige
authors.forename: Toru
authors.initials: T
authors.lastname: Kawamoto
authors.forename: Takehiro
authors.initials: T
authors.lastname: Ota
authors.forename: Shunichi
authors.initials: S
authors.lastname: Ariizumi
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamato
authors.forename: Shinichi
authors.initials: S
authors.lastname: Kadota
authors.forename: Kaoru
authors.initials: K
authors.lastname: Takeuchi
authors.forename: Akiko
authors.initials: A
authors.lastname: Ishikawa
authors.forename: Masafumi
authors.initials: M
authors.lastname: Onodera
authors.forename: Kojiro
authors.initials: K
authors.lastname: Onizawa
authors.forename: Masakazu
authors.initials: M
authors.lastname: Yamamoto
authors.forename: Eiji
authors.initials: E
authors.lastname: Miyoshi
authors.forename: Junichi
authors.initials: J
authors.lastname: Shoda
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D002277:Carcinoma / Q000098:blood supply / Q000737:chemistry* / Q000556:secondary* / Q000601:surgery; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002763:Cholecystectomy; D005260:Female; D005704:Gallbladder / Q000737:chemistry; D005706:Gallbladder Neoplasms / Q000098:blood supply / Q000737:chemistry* / Q000473:pathology* / Q000601:surgery; D015870:Gene Expression; D006801:Humans; D008113:Liver Neoplasms / Q000556:secondary*; D008207:Lymphatic Metastasis; D008297:Male; D051379:Mice; D008875:Middle Aged; D017351:N-Acetylglucosaminyltransferases / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D009367:Neoplasm Staging; D009389:Neovascularization, Pathologic; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000032:analysis; D034741:RNA, Small Interfering; D015996:Survival Rate; D047368:Tumor Burden
chemical_list: D019408:Platelet Endothelial Cell Adhesion Molecule-1; D034741:RNA, Small Interfering; D017351:N-Acetylglucosaminyltransferases; C052254:alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase