title: Antisense regulation by transposon-derived RNAs in the hyperthermophilic archaeon Sulfolobus solfataricus.
abstract: We report the first example of antisense RNA regulation in a hyperthermophilic archaeon. In Sulfolobus solfataricus, the transposon-derived paralogous RNAs, RNA-257(1-4), show extended complementarity to the 3' UTR of the 1183 mRNA, encoding a putative phosphate transporter. Phosphate limitation results in decreased RNA-257(1) and increased 1183 mRNA levels. Correspondingly, the 1183 mRNA is faster degraded in vitro upon duplex formation with RNA-257(1). Insertion of the 1183 3' UTR downstream of the lacS gene results in strongly reduced lacS mRNA levels in transformed cells, indicating that antisense regulation can function in trans.
authors.affiliation: Department of Microbiology, Immunobiology and Genetics, Max F. Perutz Laboratories, University of Vienna, Dr Bohrgasse 9, 1030 Vienna, Austria.
authors.forename: Birgit
authors.initials: B
authors.lastname: Märtens
authors.forename: Salim
authors.initials: S
authors.lastname: Manoharadas
authors.forename: David
authors.initials: D
authors.lastname: Hasenöhrl
authors.forename: Andrea
authors.initials: A
authors.lastname: Manica
authors.forename: Udo
authors.initials: U
authors.lastname: Bläsi
mesh_terms: D020413:3' Untranslated Regions; D019843:Archaeal Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D001665:Binding Sites; D004251:DNA Transposable Elements / Q000235:genetics*; D019848:Gene Expression Regulation, Archaeal; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D028061:Phosphate Transport Proteins / Q000235:genetics / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism; D034622:RNA Interference; D020871:RNA Stability; D019642:RNA, Archaeal / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D048229:Sulfolobus solfataricus / Q000235:genetics* / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; D019843:Archaeal Proteins; D004251:DNA Transposable Elements; D028061:Phosphate Transport Proteins; D010710:Phosphates; D019642:RNA, Archaeal; D012333:RNA, Messenger