title: PINK1 Deficiency Enhances Inflammatory Cytokine Release from Acutely Prepared Brain Slices.
abstract: Parkinson's disease (PD) is the second most common neurodegenerative motor disease caused by degeneration of dopaminergic neurons in the substantia nigra. Because brain inflammation has been considered a risk factor for PD, we analyzed whether PTEN induced putative kinase 1 (PINK1), an autosomal recessive familial PD gene, regulates brain inflammation during injury states. Using acutely prepared cortical slices to mimic injury, we analyzed expression of the pro-inflammatory cytokines tumor necrosis factor-α, interleukin (IL)-1β, and IL-6 at the mRNA and protein levels. Both mRNA and protein expression of these cytokines was higher at 6-24 h after slicing in PINK1 knockout (KO) slices compared to that in wild-type (WT) slices. In serial experiments to understand the signaling pathways that increase inflammatory responses in KO slices, we found that IκB degradation was enhanced but Akt phosphorylation decreased in KO slices compared to those in WT slices. In further experiments, an inhibitor of PI3K (LY294002) upstream of Akt increased expression of pro-inflammatory cytokines. Taken together, these results suggest that PINK1 deficiency enhance brain inflammation through reduced Akt activation and enhanced IκB degradation in response to brain injury.
authors.affiliation: Neuroscience Graduate Program, Ajou University School of Medicine, Suwon 442-721, Korea.
authors.forename: Jun
authors.initials: J
authors.lastname: Kim
authors.forename: Ji-Won
authors.initials: JW
authors.lastname: Byun
authors.forename: Insup
authors.initials: I
authors.lastname: Choi
authors.forename: Beomsue
authors.initials: B
authors.lastname: Kim
authors.forename: Hey-Kyeong
authors.initials: HK
authors.lastname: Jeong
authors.forename: Ilo
authors.initials: I
authors.lastname: Jou
authors.forename: Eunhye
authors.initials: E
authors.lastname: Joe
keywords: PINK1; Parkinson's disease; inflammation