title: Attenuation of erythrocytic actylcholinesterase by antidepressants: evidence in an in vitro experiment.
abstract: The current study was aimed to scrutinize acetylcholinesterase (AchE) inhibitory profile of two antidepressants, diazepam and phenobarbitone. The experimental designed was based on Michaelis-Menten parameters (apparent Michaelis constant (aKm) and apparent maximum velocity (aVm)) that estimate inhibition (%) as well as the type of inhibition (mechanism). The results showed marked inhibition of AchE by diazepam and the values of aKm and aVm were 65.5% and 52.63%, respectively. These values suggested a competitive type of antagonism for diazepam. Similar trend of antagonism was shown by phenobarbitone when it was subjected to the challenge of AchE with aKm and aVm values of 51.99% and 71.80%, respectively. It is concluded that diazepam and phenobarbitone exhibited prominent AchE attenuation apart from their well-established antidepressant activity, which could be more useful in related diseased conditions.
authors.affiliation: Department of Pharmacology, Institute of Basic Medical Sciences, Khyber Medical University, Peshawar, Pakistan shafiq_tariq2000@yahoo.com.
authors.forename: Shafiq Ahmad
authors.initials: SA
authors.lastname: Tariq
authors.affiliation: Department of Pharmacy, University of Peshawar, Peshawar, Pakistan Gandhara College of Pharmacy, Gandhara University, Peshawar, Pakistan Department of Pharmacy, Abdul Wali Khan University, Mardan, Pakistan hkdr2006@gmail.com.
authors.forename: Haroon
authors.initials: H
authors.lastname: Khan
authors.affiliation: Department of Pharmacy, University of Peshawar, Peshawar, Pakistan.
authors.forename: Naveed
authors.initials: N
authors.lastname: Muhammad
mesh_terms: D000110:Acetylcholinesterase / Q000187:drug effects*; D000928:Antidepressive Agents / Q000494:pharmacology*; D003975:Diazepam / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology*; D006801:Humans; D007700:Kinetics; D010634:Phenobarbital / Q000494:pharmacology*
chemical_list: D000928:Antidepressive Agents; D000110:Acetylcholinesterase; D003975:Diazepam; D010634:Phenobarbital
keywords: acetylcholinesterase inhibition; diazepam; phenobarbitone