title: [Supplemental role of thrombospondin-1 in the diagnosis of prostate cancer].
abstract: OBJECTIVE
  To employ enzyme-linked immunosorbent assay (ELISA) to measure the serum level of thrombospondin-1 (TSP-1) and analyze its diagnostic value for prostate cancer.
  
  
  METHODS
  The serum levels of TSP-1 were measured by human TSP-1 ELISA kit in 50 patients with organ-confined prostate cancer (n = 22) and non-organ-confined prostate cancer (n = 28). And the subjects of benign prostatic hyperplasia (BPH, n = 20) and healthy controls (n = 16) were selected.
  
  
  RESULTS
  The average serum concentration of TSP-1 was (200 ± 49) µg/L in prostate cancer group, (281 ± 53) µg/L in BPH group and (323 ± 56) µg/L in healthy control group. There were significant inter-group differences in the serum levels of TSP-1 (both P < 0.05). The average serum concentration of TSP-1 was (216 ± 34) µg/L in organ-confined prostate cancer (including stages I and II) and (188 ± 49) µg/L in non-organ-confined prostate cancer (including stages III and IV) respectively (P = 0.030). The level of TSP-1 was also correlated with Gleason score (r = -0.32, P = 0.023). However, the relationship between TSP-1 levels and lymph node metastasis remained elusive (P = 0.189). The diagnostic sensitivity and specificity of TSP-1 and prostate specific antigen (PSA) for prostate cancer were 72%, 90% and 64%, 70% respectively (both P < 0.05). The area under the receiver operating characteristic curve (ROC) of TSP-1 and PSA were 0.886 and 0.719 respectively (P = 0.028).
  
  
  CONCLUSIONS
  As a relatively ideal predictor of prostate cancer, the serum concentration of TSP-1 can not only distinguish prostate cancer from BPH, but also correlate with tumor stage and Gleason grade.
authors.affiliation: Department of Urology, Jinshan Hospital, Fudan University, Shanghai 200540, China.
authors.forename: Jian-cheng
authors.initials: JC
authors.lastname: Lu
authors.forename: Ding-wei
authors.initials: DW
authors.lastname: Ye
authors.forename: Wen-zhi
authors.initials: WZ
authors.lastname: Li
authors.forename: Li-huan
authors.initials: LH
authors.lastname: Du
authors.forename: Wei
authors.initials: W
authors.lastname: Jin
authors.forename: Xue-qiang
authors.initials: XQ
authors.lastname: Lu
authors.forename: Ji-hong
authors.initials: JH
authors.lastname: Zhang
authors.forename: Gang
authors.initials: G
authors.lastname: Chen
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D006801:Humans; D008297:Male; D008875:Middle Aged; D011471:Prostatic Neoplasms / Q000097:blood / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D019700:Thrombospondin 1 / Q000097:blood*
chemical_list: D019700:Thrombospondin 1