title: Inhibitory effect of Gastrodia elata extract on melanogenesis in HM3KO melanoma cells.
abstract: Effective and safe antimelanogenic agents derived from natural products get interest continuously for both medical and cosmetic purposes. Melanin synthesis is regulated by melanogenic enzymes such as tyrosinase, tyrosinase-related protein 1 (TRP-1), and tyrosinase-related protein 2 (TRP-2) in mammals. Gastrodia elata (GE) has been shown to have multiple therapeutic actions related to antioxidation in many diseases. In this study, we investigate whether water extraction of GE has inhibitory effects on melanogenesis in vitro and on the expression of mRNA and protein of tyrosinase, TRP-1, and TRP-2 in HM3KO melanoma cells. To examine the inhibitory effect of GE on melanogenesis, mushroom tyrosinase inhibition assay; reverse transcription polymerase chain reaction; and western blotting of tyrosinase, TRP-1, and TRP-2 were performed using HM3KO melanoma cells. In this study, the GE extract was found to significantly inhibit mushroom tyrosinase activity (69.3 ± 7.2% of the control, p < 0.05), and the expression of mRNA and protein of tyrosinase, TRP-1, and TRP-2 was reduced significantly. These results suggest that the antimelanogenic effect of GE extract is mainly due to the decreased expression of mRNA and protein of tyrosinase, TRP-1, and TRP-2 in the process of melanin synthesis.
authors.affiliation: Department of Dermatology, College of Medicine, Kyung Hee University, Seoul 130-701, Republic of Korea.
authors.forename: Hak-Ju
authors.initials: HJ
authors.lastname: Kim
authors.forename: Jong-Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: Min-Kyung
authors.initials: MK
authors.lastname: Shin
authors.forename: Kang
authors.initials: K
authors.lastname: Hyun Leem
authors.forename: Youn-Jung
authors.initials: YJ
authors.lastname: Kim
authors.forename: Mu-Hyoung
authors.initials: MH
authors.lastname: Lee
mesh_terms: D001688:Biological Products / Q000494:pharmacology*; D045744:Cell Line, Tumor; D057075:Enzyme Assays; D031668:Gastrodia / Q000737:chemistry*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D019746:Intramolecular Oxidoreductases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D008543:Melanins / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000235:genetics; D008545:Melanoma / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D008562:Membrane Glycoproteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D010088:Oxidoreductases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000037:antagonists & inhibitors* / Q000096:biosynthesis / Q000235:genetics; D012878:Skin Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology
chemical_list: D001688:Biological Products; D008543:Melanins; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D010088:Oxidoreductases; C489799:TYRP1 protein, human; D014442:Monophenol Monooxygenase; D019746:Intramolecular Oxidoreductases; C042501:dopachrome isomerase