title: Genetics of anti-EHV antibody responses in a horse population.
abstract: Individual variation in immune responses to herpesviruses was observed in various species. Here, associations between polymorphic molecular markers and life-long anti-EHV-1/4 antibody immune responses were analyzed in a model EHV-infected population of the Old Kladruber horses. Two-dimensional analysis including overall mean titers and titer dynamics expressed by differences between spring and autumn titers allowed identification of low-responders. 50 randomly selected microsatellites and nine single nucleotide polymorphisms in nine immunity-related candidate genes were genotyped. Due to differences (p<0.001) in antibody titers between two color varieties of Old Kladruber horses, separate association studies were performed in the two sub-populations by using the Fisher's exact test. In black horses, the interleukin 4 receptor and MxA protein coding genes, and the microsatellite TKY325 were associated with the responder status. In the grey population, the microsatellite TKY343 showed significant association with anti-EHV antibody responsiveness after Bonferroni corrections.
authors.affiliation: Department of Animal Genetics, University of Veterinary and Pharmaceutical Sciences, Palackého 1-3, 612 42 Brno, Czech Republic.
authors.forename: J
authors.initials: J
authors.lastname: Rusek
authors.forename: M
authors.initials: M
authors.lastname: Klumplerova
authors.forename: D
authors.initials: D
authors.lastname: Molinkova
authors.forename: M
authors.initials: M
authors.lastname: Sedlinska
authors.forename: L
authors.initials: L
authors.lastname: Dusek
authors.forename: J
authors.initials: J
authors.lastname: Muzik
authors.forename: L
authors.initials: L
authors.lastname: Putnova
authors.forename: I
authors.initials: I
authors.lastname: Vrtkova
authors.forename: V
authors.initials: V
authors.lastname: Celer
authors.forename: P
authors.initials: P
authors.lastname: Horin
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D005260:Female; D005838:Genotype; D006566:Herpesviridae Infections / Q000235:genetics / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D004861:Herpesvirus 1, Equid / Q000235:genetics / Q000276:immunology*; D006734:Horse Diseases / Q000235:genetics / Q000276:immunology* / Q000821:virology*; D006736:Horses; D018895:Microsatellite Repeats; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000662:veterinary; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D018709:Statistics, Nonparametric
chemical_list: D000914:Antibodies, Viral