title: Deoxyribonuclease activity of polyclonal IgGs: a putative serological marker in patients with spondyloarthritides.
abstract: Antibodies executing catalytic activity are referred to as antibody enzymes or short "abzymes" and may have diagnostic relevance. Abzymes with deoxyribonuclease (DNase) activity have been demonstrated in patients with autoimmune and infectious diseases. Despite several reports on the occurrence of DNase abzymes in systemic autoimmune rheumatic diseases, conclusive data about DNase activity of antibodies in patients with spondyloarthritides (SpAs) are lacking. In recent cross-sectional studies evaluating levels of IgG DNase activity in patients with psoriatic arthritis (PsA), reactive arthritis (ReA), and ankylosing spondylitis (AS), DNase activity of IgG has been assessed by the rivanol clot method and confirmed by agarose gel electrophoresis. Remarkably, levels of IgG DNase activity were significantly higher in sera of SpA patients than those in control subjects. In patients with PsA, ReA, and AS, a positive correlation of DNase IgG activity with synovitis, disease activity, and stage of spondylitis was observed, respectively. Given the involvement of autoimmune reactions in cytolysis and connective tissue degradation in PsA, ReA, and to a lesser extent in AS, abzymes might have an impact on the pathophysiology of SpAs. Detection of IgG DNase activity in patients suffering from SpA represents an exciting new research field and may assist in the differential diagnosis of SpA.
authors.affiliation: Belarusian Medical Academy of Post-graduate Education, 220013, Minsk, Belarus. elsid7@mail.ru
authors.forename: Alena V
authors.initials: AV
authors.lastname: Kundzer
authors.forename: Margarita V
authors.initials: MV
authors.lastname: Volkova
authors.forename: Dimitrios P
authors.initials: DP
authors.lastname: Bogdanos
authors.forename: Stefan
authors.initials: S
authors.lastname: Rödiger
authors.forename: Peter
authors.initials: P
authors.lastname: Schierack
authors.forename: I
authors.initials: I
authors.lastname: Generalov
authors.forename: Georgy A
authors.initials: GA
authors.lastname: Nevinsky
authors.forename: Dirk
authors.initials: D
authors.lastname: Roggenbuck
mesh_terms: D017151:Antibodies, Catalytic / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D003430:Cross-Sectional Studies; D003851:Deoxyribonucleases / Q000378:metabolism*; D003937:Diagnosis, Differential; D018450:Disease Progression; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D000091402:Prohibitins; D012698:Serologic Tests; D025242:Spondylarthropathies / Q000175:diagnosis* / Q000276:immunology
chemical_list: D017151:Antibodies, Catalytic; D015415:Biomarkers; D007074:Immunoglobulin G; C000717331:PHB2 protein, human; D000091402:Prohibitins; D003851:Deoxyribonucleases