title: Alteration of some cellular function in amikacin resistant Pseudomonas aeruginosa transfected macrophages: a time dependent approach.
abstract: OBJECTIVE
  To evaluate the free radical generation and antioxidant enzymes status in murine peritoneal macrophage during in vitro amikacin resistant Pseudomonas aeruginosa (ARPA) treatment with different time interval.
  
  
  METHODS
  Peritoneal macrophages were treated with 1×10(8) CFU/mL ARPA cell suspension in vitro for different time interval (1, 2, 3, 6, 12, and 24 h) and super oxide anion generation, NO generation, reduced glutathione level and antioxidant enzymes status were analyzed.
  
  
  RESULTS
  Super oxide anion generation and NO generation got peak at 12 h, indicating maximal free radical generation through activation of NADPH oxidase in murine peritoneal macrophages during ARPA transfection. Reduced glutathione level and antioxidant enzymes status were decreased significantly (P<0.05) with increasing time of ARPA transfection. All the changes in peritoneal macrophages after 12 h in vitro ARPA transfection had significant difference (P<0.05).
  
  
  CONCLUSIONS
  From this study, it may be summarized that in vitro ARPA infection not only generates excess free radical but also affects the antioxidant system and glutathione cycle in murine peritoneal macrophage.
authors.affiliation: Immunology and Microbiology Laboratory, Department of Human Physiology with Community Health, Vidyasagar University, Midnapore-721 102, West Bengal, India.
authors.forename: Subhankari Prasad
authors.initials: SP
authors.lastname: Chakraborty
authors.forename: Santanu
authors.initials: S
authors.lastname: Karmahapatra
authors.forename: Sabyasachi
authors.initials: S
authors.lastname: Das
authors.forename: Somenath
authors.initials: S
authors.lastname: Roy
mesh_terms: D000583:Amikacin / Q000494:pharmacology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000032:analysis; D002478:Cells, Cultured; D024881:Drug Resistance, Bacterial; D005609:Free Radicals / Q000032:analysis; D005978:Glutathione / Q000032:analysis; D017737:Macrophages, Peritoneal / Q000276:immunology* / Q000382:microbiology* / Q000502:physiology; D008297:Male; D051379:Mice; D018384:Oxidative Stress; D011550:Pseudomonas aeruginosa / Q000254:growth & development* / Q000276:immunology*; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D005609:Free Radicals; D000583:Amikacin; D005978:Glutathione
keywords: ARPA; Antioxidant enzyme; Free radical generation; Oxidative stress; Peritoneal macrophage