title: Validation of reported genetic risk factors for periodontitis in a large-scale replication study.
abstract: OBJECTIVE
  Many studies investigated the role of genetic variants in periodontitis, but few were established as risk factors. We aimed to validate the associations of recent candidate genes in aggressive periodontitis (AgP).
  
  
  METHODS
  We analysed 23 genes in 600 German AgP patients and 1441 controls on the Illumina custom genotyping array Immunochip. We tested a suggestive association in a Dutch and German/Austrian AgP case-control sample, and a German chronic periodontitis (CP) case-control sample using Sequenom iPlex assays. We additionally tested the common known risk variant rs1333048 of the gene ANRIL for its association in a Turkish and Italian population.
  
  
  RESULTS
  None of the analysed genes gave statistical evidence for association. Upon covariate adjustment for smoking and gender, in the pooled German-Austrian AgP sample, IL10 SNP rs6667202 was associated with p = 0.016, OR = 0.77 (95% CI = 0.6-0.95), and in the Dutch AgP sample, adjacent IL10 SNP rs61815643 was associated with p = 0.0009, OR = 2.31 (95% CI = 1.4-3.8). At rs61815643, binding of the transcription factor PPARG was predicted. ANRIL rs1333048 was associated in the Turkish sample (pallelic = 0.026, OR = 1.67 [95% CI = 1.11-2.60]).
  
  
  CONCLUSIONS
  Previous candidate genes carry no susceptibility factors for AgP. Association of IL-10 rs61815643 with AgP is suggested. ANRIL is associated with periodontitis across different populations.
authors.affiliation: Institute for Clinical Molecular Biology, Christian-Albrechts-University, Kiel, Germany. a.schaefer@ikmb.uni-kiel.de
authors.forename: Arne S
authors.initials: AS
authors.lastname: Schaefer
authors.forename: Gregor
authors.initials: G
authors.lastname: Bochenek
authors.forename: Thomas
authors.initials: T
authors.lastname: Manke
authors.forename: Michael
authors.initials: M
authors.lastname: Nothnagel
authors.forename: Christian
authors.initials: C
authors.lastname: Graetz
authors.forename: Anneke
authors.initials: A
authors.lastname: Thien
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Jockel-Schneider
authors.forename: Inga
authors.initials: I
authors.lastname: Harks
authors.forename: Ingmar
authors.initials: I
authors.lastname: Staufenbiel
authors.forename: Cisca
authors.initials: C
authors.lastname: Wijmenga
authors.forename: Jörg
authors.initials: J
authors.lastname: Eberhard
authors.forename: Esra
authors.initials: E
authors.lastname: Guzeldemir-Akcakanat
authors.forename: Naci
authors.initials: N
authors.lastname: Cine
authors.forename: Mathias
authors.initials: M
authors.lastname: Folwaczny
authors.forename: Barbara
authors.initials: B
authors.lastname: Noack
authors.forename: Joerg
authors.initials: J
authors.lastname: Meyle
authors.forename: Peter
authors.initials: P
authors.lastname: Eickholz
authors.forename: Leonardo
authors.initials: L
authors.lastname: Trombelli
authors.forename: Chiara
authors.initials: C
authors.lastname: Scapoli
authors.forename: Rahime
authors.initials: R
authors.lastname: Nohutcu
authors.forename: Corinna
authors.initials: C
authors.lastname: Bruckmann
authors.forename: Christof
authors.initials: C
authors.lastname: Doerfer
authors.forename: Søren
authors.initials: S
authors.lastname: Jepsen
authors.forename: Bruno G
authors.initials: BG
authors.lastname: Loos
authors.forename: Stefan
authors.initials: S
authors.lastname: Schreiber
mesh_terms: D010520:Aggressive Periodontitis / Q000235:genetics*; D001317:Austria; D001665:Binding Sites / Q000235:genetics; D016022:Case-Control Studies; D055113:Chronic Periodontitis / Q000235:genetics*; D005260:Female; D005858:Germany; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D007558:Italy; D016015:Logistic Models; D008297:Male; D009426:Netherlands; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide; D062085:RNA, Long Noncoding / Q000235:genetics*; D012307:Risk Factors; D017422:Sequence Analysis, DNA; D014421:Turkey; D044465:White People / Q000235:genetics
chemical_list: C561518:CDKN2B antisense RNA, human; D062085:RNA, Long Noncoding; D016753:Interleukin-10