title: Native human interferon-α is a strong inductor of endogenous cytokines involved in the suppression of procollagen type I.
abstract: Native human interferon-α (nHuIFN-α) has a stronger reductive effect on procollagen type I mRNA expression than recombinant human interferon-α (rHuIFN-α). It is partially due to the additive activity of interleukin-1β (IL-1β), which is present in small concentrations in nHuIFN-α. Here, we show that the reductive effect is also the result of the endogenous cytokines induced by the activity of nHuIFN-α. In the culture of MRC5 fibroblasts, we have further found that nHuIFN-α induces endogenous interferons in higher amounts than rHuIFN-α, measured with PCR. That is more pronounced when interferon-γ (IFN-γ) is measured. This result puts a new light on IFN-γ activity in the nHuIFN-α treatment because its role was neglected due to the loss of its activity during the nHuIFN-α preparation process. The findings lead to the conclusion that endogenous cytokines play a significant role in the nHuIFN-α -mediated reduction of procollagen type I mRNA and are therefore an important factor in potential therapeutic usage.
authors.affiliation: Department of Surgery, County Hospital Požega, Osječka 107, 34000 Požega, Croatia. Electronic address: gsantak@hotmail.com.
authors.forename: G
authors.initials: G
authors.lastname: Santak
authors.forename: M
authors.initials: M
authors.lastname: Santak
authors.forename: D
authors.initials: D
authors.lastname: Forčić
mesh_terms: D024042:Collagen Type I / Q000096:biosynthesis*; D003513:Cycloheximide / Q000494:pharmacology; D016207:Cytokines / Q000096:biosynthesis / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D016898:Interferon-alpha / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D007372:Interferons / Q000096:biosynthesis* / Q000187:drug effects; D053583:Interleukin-1beta / Q000378:metabolism; D011347:Procollagen / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D024042:Collagen Type I; D016207:Cytokines; D016898:Interferon-alpha; D053583:Interleukin-1beta; D011347:Procollagen; D011994:Recombinant Proteins; D007372:Interferons; D003513:Cycloheximide
keywords: Endogenous interferons; Interferon-γ; Native human interferon-α; Procollagen type I