title: Optimization of hydrogen peroxide detection for a methyl mercaptan biosensor.
abstract: Several kinds of modified carbon screen printed electrodes (CSPEs) for amperometric detection of hydrogen peroxide (H₂O₂) are presented in order to propose a methyl mercaptan (MM) biosensor. Unmodified, carbon nanotubes (CNTs), cobalt phthalocyanine (CoPC), Prussian blue (PB), and Os-wired HRP modified CSPE sensors were fabricated and tested to detect H₂O₂, applying a potential of +0.6 V, +0.6 V, +0.4 V, -0.2 V and -0.1 V (versus Ag/AgCl), respectively. The limits of detection of these electrodes for H₂O₂ were 3.1 μM, 1.3 μM, 71 nM, 1.3 μM, 13.7 nM, respectively. The results demonstrated that the Os-wired HRP modified CSPEs gives the lowest limit of detection (LOD) for H₂O₂ at a working potential as low as -0.1 V. Os-wired HRP is the optimum choice for establishment of a MM biosensor and gives a detection limit of 0.5 μM.
authors.affiliation: State Key Laboratory of Physical Chemistry of Solid Surfaces, and Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
authors.forename: Zhan-Hong
authors.initials: ZH
authors.lastname: Li
authors.forename: Houssemeddine
authors.initials: H
authors.lastname: Guedri
authors.forename: Bruno
authors.initials: B
authors.lastname: Viguier
authors.forename: Shi-Gang
authors.initials: SG
authors.lastname: Sun
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Marty
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D002384:Catalysis; D055664:Electrochemical Techniques; D004566:Electrodes; D005295:Ferrocyanides / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000032:analysis*; D007211:Indoles / Q000737:chemistry; D037742:Nanotubes, Carbon / Q000737:chemistry; D009942:Organometallic Compounds / Q000737:chemistry; D009992:Osmium / Q000737:chemistry; D010084:Oxidation-Reduction; D013438:Sulfhydryl Compounds / Q000032:analysis*
chemical_list: D005295:Ferrocyanides; D007211:Indoles; D037742:Nanotubes, Carbon; D009942:Organometallic Compounds; D013438:Sulfhydryl Compounds; D009992:Osmium; C005231:methylmercaptan; C063633:cobalt phthalocyanine; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; C000170:ferric ferrocyanide