title: Expression and redistribution of β-catenin in the cardiac myocytes of left ventricle of spontaneously hypertensive rat.
abstract: Beta-catenin is not only an adhering junction protein, but also the central player of the canonical Wnt signalling pathway. In order to investigate the roles of β-catenin in the mechanism of myocardial hypertrophy, we determined the expression and distribution of β-catenin in the cardiomyocytes of spontaneously hypertensive heart failure (SHHF) rats and age-matched Wistar-Kyoto (WKY) rats. We identified the reducing of β-catenin expression in the membrane protein fraction but increasing in the nuclear protein in the 6 and 12 month-old SHHF rats as compared with the age-matched WKY rats by Western blotting. Immunolabeling of β-catenin colocalized with cadherin at the intercalated disc sites and showed nuclear accumulation in myocytes of SHHF rats. We also revealed that the association between glycogen synthase kinase-3β and β-catenin had weakened in the 6 month-old SHHF rats as compared with the age-matched WKY rats by immunoprecipitation. These findings suggested that nuclear translocation of β-catenin might play important roles in regulating signal transduction in the decompensated hypertrophy stage.
authors.affiliation: Department of Pathology, Sun Yat-sen University the Fifth Affiliated Hospital, 52 Meihua E. Road, Zhuhai, 519000, Guangdong, People's Republic of China.
authors.forename: Qiaoli
authors.initials: Q
authors.lastname: Zheng
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.forename: Zeqing
authors.initials: Z
authors.lastname: Xu
authors.forename: Faqian
authors.initials: F
authors.lastname: Li
authors.forename: Xian Ping
authors.initials: XP
authors.lastname: Yi
mesh_terms: D021581:Active Transport, Cell Nucleus; D000367:Age Factors; D000818:Animals; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D015870:Gene Expression; D038362:Glycogen Synthase Kinase 3 / Q000235:genetics / Q000378:metabolism; D000071679:Glycogen Synthase Kinase 3 beta; D006352:Heart Ventricles / Q000378:metabolism* / Q000473:pathology; D006973:Hypertension / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D017379:Hypertrophy, Left Ventricular / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D032383:Myocytes, Cardiac / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D060449:Wnt Signaling Pathway; D051176:beta Catenin / Q000235:genetics* / Q000378:metabolism
chemical_list: D015820:Cadherins; C495268:Ctnnb1 protein, rat; D051176:beta Catenin; D000071679:Glycogen Synthase Kinase 3 beta; C000605508:Gsk3b protein, rat; D038362:Glycogen Synthase Kinase 3