title: Rutin and quercetin, bioactive compounds from tartary buckwheat, prevent liver inflammatory injury.
abstract: Tartary buckwheat (Fagopyrum tataricum) is a healthy and nutritionally important food item. In this study, we investigated the hepatoprotective effects of 75% ethanol extracts from tartary buckwheat (EEB) against ethanol- and carbon tetrachloride (CCl(4))-induced liver damage. EEB were administered to C57BL/6 mice (ethanol induction) and Sprague-Dawley (SD) rats (CCl(4) induction) for 4 and 8 consecutive weeks, respectively. The major active compounds, rutin and quercetin, were also administered to ethanol- and CCl(4)-induced animals. EEB inhibited increase in serum aspartate transaminase (AST), alanine transaminase (ALT) and alkaline phosphatase (ALP) levels in the ethanol- and CCl(4)-induced animals; similar effects were found after rutin and quercetin administration. Moreover, EEB elevated the antioxidant enzyme activities, including those of catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), and superoxide dismutase (SOD), and inhibited the levels of hepatic inflammation in the ethanol- and CCl(4)-treated animals. This study suggests that EEB exerts hepatoprotection via promoting anti-oxidative and anti-inflammatory properties against oxidative liver damage.
authors.affiliation: Department of Food Science, National Chiayi University, No 300 Syue fu Rd, Chiayi City 60004, Taiwan, ROC.
authors.forename: Chia-Chen
authors.initials: CC
authors.lastname: Lee
authors.forename: Siou-Ru
authors.initials: SR
authors.lastname: Shen
authors.forename: Ying-Jang
authors.initials: YJ
authors.lastname: Lai
authors.forename: She-Ching
authors.initials: SC
authors.lastname: Wu
mesh_terms: D000410:Alanine Transaminase / Q000037:antagonists & inhibitors / Q000097:blood; D000469:Alkaline Phosphatase / Q000037:antagonists & inhibitors / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001219:Aspartate Aminotransferases / Q000037:antagonists & inhibitors / Q000097:blood; D002251:Carbon Tetrachloride / Q000009:adverse effects; D002374:Catalase / Q000097:blood; D000431:Ethanol / Q000009:adverse effects / Q000378:metabolism; D019612:Fagopyrum / Q000737:chemistry*; D005979:Glutathione Peroxidase / Q000097:blood; D005980:Glutathione Reductase / Q000097:blood; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D011794:Quercetin / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species; D012431:Rutin / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D000431:Ethanol; D012431:Rutin; D011794:Quercetin; D002251:Carbon Tetrachloride; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase