title: Cellular stress induced by photodynamic reaction with CoTPPS and MnTMPyPCl5 in combination with electroporation in human colon adenocarcinoma cell lines (LoVo and LoVoDX).
abstract: Two porphyrins, CoTPPS and MnTMPyPCl5, were tested for their photodynamic activity and potential novel use in a therapy of human cancers. We investigated an effect of photodynamic reaction (PDR), electroporation (EP) and their combination (electro-photodynamic reaction [EP-PDR]) on human colon adenocarcinoma cell lines (LoVo and resistant to doxorubicin LoVoDX), human breast adenocarcinoma (wild type MCF-7/WT and resistant to doxorubicin MCF-7/DOX), and human melanoma (Me45). The efficiency of macromolecules transport was examined with cytofluorymetry by assessing the degree of propidium iodide (PI) penetration. Additionally, cellular ultrastructure after EP was evaluated. We determined cyto- and photo-cytotoxic effect on the cells viability (MTT assay) after standard PDR and PDR combined with EP. Intracellular distribution and mitochondrial colocalization of both porphyrins was also performed. The experiments proved that both complexes exhibit desirable photodynamic properties on LoVo LoVoDX cells, and EP effectively supports photodynamic method in this type of cancer. The application of EP provided shorter time of incubation (only 10 min) and enhanced effect of applied therapy. The porphyrins did not affect the MCF-7 and Me45 cell lines.
authors.affiliation: Department of Medical Biochemistry, Medical University, Chalubinskiego 10, 50-368, Wroclaw, Poland, Julita.Kulbacka@umed.wroc.pl.
authors.forename: J
authors.initials: J
authors.lastname: Kulbacka
authors.forename: M
authors.initials: M
authors.lastname: Kotulska
authors.forename: N
authors.initials: N
authors.lastname: Rembiałkowska
authors.forename: A
authors.initials: A
authors.lastname: Choromańska
authors.forename: I
authors.initials: I
authors.lastname: Kamińska
authors.forename: A
authors.initials: A
authors.lastname: Garbiec
authors.forename: J
authors.initials: J
authors.lastname: Rossowska
authors.forename: M
authors.initials: M
authors.lastname: Daczewska
authors.forename: B
authors.initials: B
authors.lastname: Jachimska
authors.forename: J
authors.initials: J
authors.lastname: Saczko
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D056831:Coordination Complexes / Q000737:chemistry / Q000633:toxicity*; D004317:Doxorubicin / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm / Q000187:drug effects; D018274:Electroporation; D006801:Humans; D008027:Light; D061986:MCF-7 Cells; D008665:Metalloporphyrins / Q000737:chemistry / Q000633:toxicity*; D009942:Organometallic Compounds / Q000737:chemistry / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000633:toxicity*; D011166:Porphyrins / Q000737:chemistry / Q000633:toxicity*; D011419:Propidium / Q000737:chemistry / Q000378:metabolism
chemical_list: D056831:Coordination Complexes; D008665:Metalloporphyrins; D009942:Organometallic Compounds; D017319:Photosensitizing Agents; D011166:Porphyrins; C114728:manganese(II) 5,10,15,20-tetrakis(4-N-methylpyridine)porphyrin; D011419:Propidium; D004317:Doxorubicin; C056134:cobalt(III)-tetrakis(4-sulfonatophenyl)porphyrin