title: Construction of mobilizable mini-Tn7 vectors for bioluminescent detection of gram-negative bacteria and single-copy promoter lux reporter analysis.
abstract: We describe the construction of mini-Tn7-based broad-host-range vectors encoding lux genes as bioluminescent reporters. These constructs can be mobilized into the desired host(s) by conjugation for chromosomal mini-Tn7-lux integration and are useful for localization of bacteria during infections or for characterizing regulation of promoters of interest in Gram-negative bacteria.
authors.affiliation: Department of Microbiology, Immunology, and Cancer Biology, University of Virginia Health System, Charlottesville, VA, USA.
authors.forename: F Heath
authors.initials: FH
authors.lastname: Damron
authors.forename: Elizabeth S
authors.initials: ES
authors.lastname: McKenney
authors.forename: Mariette
authors.initials: M
authors.lastname: Barbier
authors.forename: George W
authors.initials: GW
authors.lastname: Liechti
authors.forename: Herbert P
authors.initials: HP
authors.lastname: Schweizer
authors.forename: Joanna B
authors.initials: JB
authors.lastname: Goldberg
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D002874:Chromosome Mapping; D017931:DNA Primers / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods*; D017930:Genes, Reporter / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics*; D006090:Gram-Negative Bacteria / Q000235:genetics* / Q000302:isolation & purification*; D049453:Luciferases, Bacterial / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011550:Pseudomonas aeruginosa / Q000378:metabolism; D014296:Trimethoprim Resistance / Q000235:genetics / Q000502:physiology
chemical_list: D015415:Biomarkers; D017931:DNA Primers; D049453:Luciferases, Bacterial