title: A RP-HPLC method for quantification of diclofenac sodium released from biological macromolecules.
abstract: Interpenetrating network (IPN) microbeads of sodium carboxymethyl locust bean gum (SCMLBG) and sodium carboxymethyl cellulose (SCMC) containing diclofenac sodium (DS), a nonsteroidal anti-inflammatory drug, were prepared by single water-in-water (w/w) emulsion gelation process using AlCl3 as cross-linking agent in a complete aqueous environment. Pharmacokinetic study of these IPN microbeads was then carried out by a simple and feasible high-performance liquid chromatographic method with UV detection which was developed and validated for the quantification of diclofenac sodium in rabbit plasma. The chromatographic separation was carried out in a Hypersil BDS, C18 column (250 mm × 4.6 mm; 5 m). The mobile phase was a mixture of acetonitrile and methanol (70:30, v/v) at a flow rate of 1.0 ml/min. The UV detection was set at 276 nm. The extraction recovery of diclofenac sodium in plasma of three quality control (QC) samples was ranged from 81.52% to 95.29%. The calibration curve was linear in the concentration range of 20-1000 ng/ml with the correlation coefficient (r(2)) above 0.9951. The method was specific and sensitive with the limit of quantification of 20 ng/ml. In stability tests, diclofenac sodium in rabbit plasma was stable during storage and assay procedure.
authors.affiliation: School of Pharmaceutical Sciences, IFTM University, Moradabad, Uttar Pradesh, India. shivsankar.bhattacharya@gmail.co.in
authors.forename: Shiv Sankar
authors.initials: SS
authors.lastname: Bhattacharya
authors.forename: Subham
authors.initials: S
authors.lastname: Banerjee
authors.forename: Ashoke Kumar
authors.initials: AK
authors.lastname: Ghosh
authors.forename: Pronobesh
authors.initials: P
authors.lastname: Chattopadhyay
authors.forename: Anurag
authors.initials: A
authors.lastname: Verma
authors.forename: Amitava
authors.initials: A
authors.lastname: Ghosh
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry* / Q000302:isolation & purification / Q000493:pharmacokinetics; D019540:Area Under Curve; D001774:Blood Chemical Analysis / Q000592:standards; D002138:Calibration; D002266:Carboxymethylcellulose Sodium / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000592:standards; D056148:Chromatography, Reverse-Phase / Q000592:standards; D003692:Delayed-Action Preparations / Q000737:chemistry / Q000302:isolation & purification / Q000493:pharmacokinetics; D004008:Diclofenac / Q000737:chemistry* / Q000302:isolation & purification / Q000493:pharmacokinetics; D004337:Drug Carriers / Q000737:chemistry* / Q000302:isolation & purification / Q000493:pharmacokinetics; D004355:Drug Stability; D005685:Galactans / Q000737:chemistry*; D006207:Half-Life; D008351:Mannans / Q000737:chemistry*; D008863:Microspheres; D053149:Plant Gums / Q000737:chemistry*; D011817:Rabbits; D012015:Reference Standards
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D005685:Galactans; D008351:Mannans; D053149:Plant Gums; D004008:Diclofenac; D002266:Carboxymethylcellulose Sodium; C017471:locust bean gum