title: Label-free and selective bacteria detection using a film with transferred bacterial configuration.
abstract: Specific identification of bacteria has been achieved through precisely transferred bacterial structure on the surface of overoxidized polypyrrole (OPPy) film. The recognition of target bacteria was successfully carried out in real time using OPPy film in combination with dielectrophoresis. The unique combination of both techniques made the specific detection of template bacilli possible at concentrations as low as 10(3) CFU/mL within 3 min, without any bacterial pretreatment. The observation of the movement of bacteria by using a fluorescent microscope revealed that living bacteria were being trapped vertically in the cavity created in the OPPy film. Further, the bacterial cavities had high selectivity and were able to discriminate particular target bacteria, Pseudomonas aeruginosa, out of bacterial mixtures containing Acinetobacter calcoaceticus, Escherichia coli, and Serratia marcescens, which are known to have a similar shape. This simple method can be used for a wide variety of applications in which rapid bacterial detection is required, such as food safety risk assessment, clinical point-of-care testing, and continuous environmental monitoring.
authors.affiliation: Nanoscience and Nanotechnology Research Center, Research Organization for the 21st Century, Osaka Prefecture University, Naka-ku, Sakai, Japan. s-tokonami@21c.osakafu-u.ac.jp
authors.forename: Shiho
authors.initials: S
authors.lastname: Tokonami
authors.forename: Yu
authors.initials: Y
authors.lastname: Nakadoi
authors.forename: Mari
authors.initials: M
authors.lastname: Takahashi
authors.forename: Mugihei
authors.initials: M
authors.lastname: Ikemizu
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Kadoma
authors.forename: Kenta
authors.initials: K
authors.lastname: Saimatsu
authors.forename: Le Quynh
authors.initials: le Q
authors.lastname: Dung
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shiigi
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Nagaoka
mesh_terms: D004563:Electrochemistry; D004586:Electrophoresis / Q000379:methods*; D054802:Molecular Imprinting; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry; D011550:Pseudomonas aeruginosa / Q000737:chemistry* / Q000302:isolation & purification*; D011758:Pyrroles / Q000138:chemical synthesis / Q000737:chemistry
chemical_list: D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole