title: Titanium surface coating with a laminin-derived functional peptide promotes bone cell adhesion.
abstract: Laminin-derived peptide coatings can enhance epithelial cell adhesion to implants, and the positive effect of these peptides on bone cell adhesion has been anticipated. The purpose of this study was to evaluate the improvement in bone cell attachment to and activity on titanium (Ti) scaffolds coated with a laminin-derived functional peptide, Ln2-P3 (the DLTIDDSYWYRI motif). Four Ti disc surfaces were prepared, and a human osteosarcoma (HOS) cell attachment test was performed to select two candidate surfaces for peptide coating. These two candidates were then coated with Ln2-P3 peptide, a scrambled peptide, or left uncoated to measure cell attachment to each surface, following which one surface was chosen to assess alkaline phosphatase (ALP) activity and osteogenic marker gene expression with quantitative real-time PCR. On the commercially pure Ti surface, the Ln2-P3 coating significantly increased cellular ALP activity and the expression levels of ALP and bone sialoprotein mRNA as compared with the scrambled peptide-coated and uncoated surfaces. In conclusion, although further in vivo studies are needed, the findings of this in vitro study indicate that the Ln2-P3-coated implant surface promotes bone cell adhesion, which has clinical implications for reducing the overall treatment time of dental implant therapy.
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University, Seoul 110-749, Republic of Korea.
authors.forename: Seung-Ki
authors.initials: SK
authors.lastname: Min
authors.forename: Hyun Ki
authors.initials: HK
authors.lastname: Kang
authors.forename: Da Hyun
authors.initials: DH
authors.lastname: Jang
authors.forename: Sung Youn
authors.initials: SY
authors.lastname: Jung
authors.forename: O Bok
authors.initials: OB
authors.lastname: Kim
authors.forename: Byung-Moo
authors.initials: BM
authors.lastname: Min
authors.forename: In-Sung
authors.initials: IS
authors.lastname: Yeo
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects*; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D020099:Coated Materials, Biocompatible / Q000008:administration & dosage / Q000737:chemistry; D015921:Dental Implants; D006801:Humans; D007797:Laminin / Q000737:chemistry; D010012:Osteogenesis; D012516:Osteosarcoma / Q000378:metabolism; D010455:Peptides / Q000008:administration & dosage / Q000737:chemistry; D013499:Surface Properties; D014025:Titanium / Q000008:administration & dosage / Q000737:chemistry
chemical_list: D020099:Coated Materials, Biocompatible; D015921:Dental Implants; D007797:Laminin; D010455:Peptides; D014025:Titanium