title: The human TREX-2 complex is stably associated with the nuclear pore basket.
abstract: In eukaryotes, mRNA export involves many evolutionarily conserved factors that carry the nascent transcript to the nuclear pore complex (NPC). The THO/TREX complex couples transcription to mRNA export and recruits the mRNA export receptor NXF1 for the transport of messenger ribonucleoprotein particles (mRNP) to the NPC. The transcription and export complex 2 (TREX-2) was suggested to interact with NXF1 and to shuttle between transcription sites and the NPC. Here, we characterize the dynamics of human TREX-2 and show that it stably associates with the NPC basket. Moreover, the association of TREX-2 with the NPC requires the basket nucleoporins NUP153 and TPR, but is independent of transcription. Differential profiles of mRNA nuclear accumulation reveal that TREX-2 functions similarly to basket nucleoporins, but differently from NXF1. Thus, our results show that TREX-2 is an NPC-associated complex in mammalian cells and suggest that it is involved in putative NPC basket-related functions.
authors.affiliation: Cellular Signaling and Nuclear Dynamics Program, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS UMR 7104, INSERM U964, Université de Strasbourg, BP 10142 - 67404 ILLKIRCH Cedex, CU de Strasbourg, France.
authors.forename: David
authors.initials: D
authors.lastname: Umlauf
authors.forename: Jacques
authors.initials: J
authors.lastname: Bonnet
authors.forename: François
authors.initials: F
authors.lastname: Waharte
authors.forename: Marjorie
authors.initials: M
authors.lastname: Fournier
authors.forename: Matthieu
authors.initials: M
authors.lastname: Stierle
authors.forename: Benoit
authors.initials: B
authors.lastname: Fischer
authors.forename: Laurent
authors.initials: L
authors.lastname: Brino
authors.forename: Didier
authors.initials: D
authors.lastname: Devys
authors.forename: László
authors.initials: L
authors.lastname: Tora
mesh_terms: D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D005090:Exodeoxyribonucleases / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D022022:Nuclear Pore / Q000235:genetics / Q000378:metabolism*; D028861:Nuclear Pore Complex Proteins / Q000235:genetics / Q000378:metabolism*; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D029543:Nucleocytoplasmic Transport Proteins / Q000235:genetics / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D034443:RNA Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics / Q000502:physiology
chemical_list: C527171:Eny2 protein, human; D047908:Intracellular Signaling Peptides and Proteins; C079377:NUP153 protein, human; C122111:NXF1 protein, human; D028861:Nuclear Pore Complex Proteins; D009687:Nuclear Proteins; D029543:Nucleocytoplasmic Transport Proteins; D010750:Phosphoproteins; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C073597:TPR protein, human; D014157:Transcription Factors; D000123:Acetyltransferases; C406825:MCM3AP protein, human; D005090:Exodeoxyribonucleases; C486104:TREX2 protein, human
keywords: AMEX; NXF1; Nuclear pore complex; THO/TREX; TREX-2; Transcription; mRNA export