title: Identification of a novel antimicrobial peptide from Brazilian coast coral Phyllogorgia dilatata.
abstract: The marine ecosystem is able to provide enormous biomolecule diversity that could be used for treatment of various diseases. In this highly competitive environment, organisms need chemical barriers to reduce or avoid microorganism contamination. Among the molecules that protect these animals the antimicrobial peptides (AMPs) are included. In the present study, crude extracts of coral coral specimens Carijoa riisei, Muriceopsis sulphurea, Neospongodes atlantica, Palythoa caribeorum, Phyllogorgia dilatata and Plexaurella grandiflora were challenged against multiple Grampositive and -negative bacteria showing different activities. P. dilatata crude extract showed the antibacterial activity, and was ammonium-sulfate (0-40%) fractionated, being able to control the growth of K. pneumoniae, S. flexineri and S. aureus. Rich-fraction was further purified by using Amicon® Ultra Centrifugal 10 kDa associated with reversed-phase HPLC chromatography (C18), producing the peptide named Pd-AMP1. Pd-AMP1 was able to inhibit S. aureus development. Mass spectrometry analyses showed a monoisotopic mass of 5372.66 Da and N-terminal sequence showed no significant match with databank. In this view, the prospecting of protein biomolecules and biotechnological potential from marine animals is still little explored and may serve as an alternative to common antibiotics.
authors.affiliation: Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-Graduaçao em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília.
authors.forename: Loiane Alves
authors.initials: LA
authors.lastname: de Lima
authors.forename: Ludovico
authors.initials: L
authors.lastname: Migliolo
authors.forename: Clovis
authors.initials: C
authors.lastname: Barreiro e Castro
authors.forename: Debora de Oliveira
authors.initials: Dde O
authors.lastname: Pires
authors.forename: Carlos
authors.initials: C
authors.lastname: Lopez-Abarrategui
authors.forename: Eveline Ferreira
authors.initials: EF
authors.lastname: Gonçalves
authors.forename: Ilka Maria
authors.initials: IM
authors.lastname: Vasconcelos
authors.forename: Jose Tadeu Abreu
authors.initials: JT
authors.lastname: de Oliveira
authors.forename: Anselmo de Jesus
authors.initials: Ade J
authors.lastname: Otero-Gonzalez
authors.forename: Octávio Luiz
authors.initials: OL
authors.lastname: Franco
authors.forename: Simoni Campos
authors.initials: SC
authors.lastname: Dias
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D001424:Bacterial Infections / Q000188:drug therapy; D001938:Brazil; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D010455:Peptides