title: Laser microdissection and saturation labeling DIGE method for the analysis of human arteries.
abstract: Laser microdissection (LMD) is a novel methodology for noncontact isolation of tissue regions or cells for subsequent molecular analysis. Although it is an upcoming field, its combination with proteomics for differential analysis remains not very well explored, since amount of protein obtained after LMD is scarce. We have combined LMD arterial layer isolation with saturation labeling DIGE, successfully achieving differential analysis of healthy and pathological intima and media layers. Identification of differential spots could be performed in whole tissue extract as reference proteome, since studied regions are subproteomes of the aforementioned.
authors.affiliation: Department of Vascular Physiopathology, Hospital Nacional de Parapléjicos, SESCAM, Toledo, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: de la Cuesta
authors.forename: Gloria
authors.initials: G
authors.lastname: Alvarez-Llamas
authors.forename: Aroa S
authors.initials: AS
authors.lastname: Maroto
authors.forename: Maria G
authors.initials: MG
authors.lastname: Barderas
authors.forename: Fernando
authors.initials: F
authors.lastname: Vivanco
mesh_terms: D017209:Apoptosis; D001158:Arteries / Q000378:metabolism* / Q000473:pathology* / Q000503:physiopathology; D001777:Blood Coagulation; D003324:Coronary Artery Disease / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D005109:Extracellular Matrix / Q000378:metabolism; D018869:Heat-Shock Response; D006470:Hemorrhage / Q000378:metabolism / Q000473:pathology; D006801:Humans; D061346:Laser Capture Microdissection / Q000379:methods*; D013194:Staining and Labeling; D053719:Tandem Mass Spectrometry; D017539:Tunica Intima / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D017540:Tunica Media / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D057825:Two-Dimensional Difference Gel Electrophoresis / Q000379:methods*