title: Development of EST-based new SSR markers in seabuckthorn.
abstract: EST-based SSR markers were developed by screening a collection of 1584 clustered ESTs of seabuckthorn (Hippophae rhamnoides). PCR primers were designed for the amplification of 30 microsatellite loci. Two to five allelic bands were displayed by nine primer pairs in H. rhamnoides genotypes and by eleven primer pairs in H. salicifolia genotypes. None of the thirty primer pairs detected polymorphism in H. tibetana genotypes. Considering the high polymorphism detected in the tested genotypes and their direct origin from the genic regions, these EST-SSR markers hold immense promise in seabuckthorn genome analysis, molecular breeding and population genetics.
authors.affiliation: University School of Biotechnology, Guru Gobind Singh Indraprastha University, Kashmere Gate, Delhi, 110403 India.
authors.forename: Ankit
authors.initials: A
authors.lastname: Jain
authors.forename: Rajesh
authors.initials: R
authors.lastname: Ghangal
authors.forename: Atul
authors.initials: A
authors.lastname: Grover
authors.forename: Saurabh
authors.initials: S
authors.lastname: Raghuvanshi
authors.forename: Prakash C
authors.initials: PC
authors.lastname: Sharma
keywords: Cross-amplification; Expressed sequence tags; Hippophae rhamnoides; Simple sequence repeats (SSRs)