title: Effect of chlorhexidine on the bond strength of a self-etch adhesive system to sound and demineralized dentin.
abstract: This study evaluated the effect of a 2% chlorhexidine-based disinfectant (CHX) on the short-term resin-dentin bond strength of a self-etch adhesive system to human dentin with different mineral contents. Dentinal mineralization was tested at 4 levels (sound, and after 2, 4, or 8 days of demineralization-remineralization cycles) and disinfectant at 2 levels [deionized water (DW, negative control) and CHX]. Dentin demineralization induced by pH-cycling was characterized by cross-sectional hardness (CSH). Each dentin surface was divided into halves, one treated with DW and the other with CHX (5 minutes). Each surface was bonded with a self-etch adhesive system and restored. The specimens were sectioned and subjected to microtensile bond testing. CSH and microtensile bond strength (µTBS) data were analyzed by regression analysis and ANOVA-Tukey tests (α = 5%), respectively. The groups treated with CHX resulted in mean µTBS similar to those found for the groups in which the dentin was exposed to DW (p = 0.821). However, mean µTBS were strongly influenced by dentin mineralization (p < 0.05): the bond strength found for sound dentin was lower than that found for dentin cycled for 8 days, which was even lower than the bond strengths for dentin cycled for 2 or 4 days. The results suggest that the degree of dentin demineralization affects the bond strength of self-etching adhesives, but the use of CHX does not modify this effect.
authors.affiliation: Faculty of Pharmacy, Dentistry and Nursing, Univ Federal do Ceará, Fortaleza, CE, Brazil.
authors.forename: Mary Anne Sampaio
authors.initials: MA
authors.lastname: de-Melo
authors.forename: Diego da Costa
authors.initials: Dda C
authors.lastname: Goes
authors.forename: Maria Denise Rodrigues
authors.initials: MD
authors.lastname: de-Moraes
authors.forename: Sérgio Lima
authors.initials: SL
authors.lastname: Santiago
authors.forename: Lidiany Karla Azevedo
authors.initials: LK
authors.lastname: Rodrigues
mesh_terms: D000704:Analysis of Variance; D002710:Chlorhexidine / Q000737:chemistry / Q000494:pharmacology*; D001840:Dental Bonding / Q000379:methods*; D003804:Dentin / Q000187:drug effects*; D057133:Dentin Desensitizing Agents / Q000737:chemistry / Q000494:pharmacology*; D006245:Hardness Tests; D006801:Humans; D008422:Materials Testing; D013499:Surface Properties; D013718:Tensile Strength / Q000187:drug effects; D013997:Time Factors; D017001:Tooth Demineralization; D014082:Tooth Fractures
chemical_list: D057133:Dentin Desensitizing Agents; D002710:Chlorhexidine