title: Chromatographic finger print analysis of steroids in Aerva lanata L by HPTLC technique.
abstract: OBJECTIVE
  To determine the chemical profile and steroids composition of the medicinally important plant Aerva lanata (A. lanata) L.
  
  
  METHODS
  Preliminary phytochemical screening was done by the method as Harborne described. HPTLC studies were carried out as Harborne and Wagner et al described. The Ethyl acetate-ethanol-water (8: 2: 1.2) was employed as mobile phase for glycosides.
  
  
  RESULTS
  The desired aim was achieved using Chloroform-acetone (8: 2) as the mobile phase. The methanolic extract of stem, leaves, root, flower and seeds of A. lanata showed the presence of 30 different types of steroids with 30 different Rf values from 0.04 to 0.97. Maximum number (11) of steroids has been observed in leaves followed by root (10).
  
  
  CONCLUSIONS
  HPTLC profile of steroids has been chosen here to reveal the diversity existing in A. lanata. Such finger printing is useful in differentiating the species from the adulterant and act as biochemical markers for this medicinally important plant in the pharma industry and plant systematic studies.
authors.affiliation: Research & Development Department, Bharathiyar University, Coimbatore-641 046, Tamil Nadu, India.
authors.forename: Mariswamy
authors.initials: M
authors.lastname: Yamunadevi
authors.forename: Edward Gnaraj
authors.initials: EG
authors.lastname: Wesely
authors.forename: M
authors.initials: M
authors.lastname: Johnson
mesh_terms: D027703:Amaranthaceae / Q000737:chemistry*; D002855:Chromatography, Thin Layer / Q000379:methods*; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D010946:Plants, Medicinal / Q000737:chemistry*; D013256:Steroids / Q000032:analysis*
chemical_list: D010936:Plant Extracts; D013256:Steroids
keywords: Aerva lanata L.; Fingerprint; HPTLC profile; Steroids