title: Induction of rat liver tumor using the Sleeping Beauty transposon and electroporation.
abstract: The Sleeping Beauty (SB) transposon system has been receiving much attention as a gene transfer method of choice since it allows permanent gene expression after insertion into the host chromosome. However, low transposition frequency in higher eukaryotes limits its use in commonly-used mammalian species. Researchers have therefore attempted to modify gene delivery and expression to overcome this limitation. In mouse liver, tumor induction using SB introduced by the hydrodynamic method has been successfully accomplished. Liver tumor in rat models using SB could also be of great use; however, dose of DNA, injection volume, rate of injection and achieving back pressure limit the use of the hydrodynamics-based gene delivery. In the present study, we combined the electroporation, a relatively simple and easy gene delivery method, with the SB transposon system and as a result successfully induced tumor in rat liver by directly injecting the c-Myc, HRAS and shp53 genes. The tumor phenotype was determined as a sarcomatoid carcinoma. To our knowledge, this is the first demonstration of induction of tumor in the rat liver using the electroporation-enhanced SB transposon system.
authors.affiliation: Asan Institute for Life Sciences, University of Ulsan College of Medicine, Asan Medical Center, Seoul, South Korea.
authors.forename: June-Shine
authors.initials: JS
authors.lastname: Park
authors.forename: Bae-Hwan
authors.initials: BH
authors.lastname: Kim
authors.forename: Sung Goo
authors.initials: SG
authors.lastname: Park
authors.forename: Sun Young
authors.initials: SY
authors.lastname: Jung
authors.forename: Do Hee
authors.initials: DH
authors.lastname: Lee
authors.forename: Woo-Chan
authors.initials: WC
authors.lastname: Son
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D004251:DNA Transposable Elements; D018274:Electroporation; D005260:Female; D007150:Immunohistochemistry; D008114:Liver Neoplasms, Experimental / Q000000981:diagnostic imaging / Q000235:genetics*; D008297:Male; D049268:Positron-Emission Tomography; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D004251:DNA Transposable Elements