title: Characteristic variation of α-fetoprotein DNA nanometer-range irradiated by iodine-125.
abstract: To obtain the characteristic variation of structure and functional groups of α-fetoprotein (AFP) DNA irradiated by iodine-125((125)I), the AFP antisense oligonucleotide labeled with various radioactivity dose (125)I was mixed with the AFP DNA in a simulated polymerase chain reaction temperature condition. After the mixtures were irradiated by the (125)I from 2 to 72 hours, the mutation of the biogenic conformation and functional groups of the irradiated DNA were investigated using laser Raman spectroscopy. The shifted peak and the decreased intensity of the characteristic Raman spectra were found, which demonstrated that the structure of the phosphodiester linkage was broke, the pyridine and purine bases in DNA emerged and damaged. The model of gene conformation changed from form B to form C spectrum after the nanometer-range irradiation with (125)I from 2 to 24 hours. The damage of local pyridine and purine bases gradually increased along with the accumulation of irradiation, and the bases and ribosome were finally dissociated and stacked.
authors.affiliation: Department of Nuclear Medicine, Third Hospital Affiliated Sun Yat-Sen University, Guangzhou, China.
authors.forename: Mu-hua
authors.initials: MH
authors.lastname: Cheng
authors.forename: Yao-xiong
authors.initials: YX
authors.lastname: Huang
authors.forename: Jian-fang
authors.initials: JF
authors.lastname: Li
authors.forename: Zheng-jie
authors.initials: ZJ
authors.lastname: Wu
authors.forename: Liang-jun
authors.initials: LJ
authors.lastname: Xie
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000528:radiation effects; D004249:DNA Damage; D015316:Genetic Therapy / Q000379:methods; D006801:Humans; D007457:Iodine Radioisotopes / Q000737:chemistry*; D016376:Oligonucleotides, Antisense / Q000737:chemistry / Q000235:genetics; D011878:Radiotherapy / Q000379:methods; D013059:Spectrum Analysis, Raman; D000509:alpha-Fetoproteins / Q000737:chemistry* / Q000235:genetics*
chemical_list: D007457:Iodine Radioisotopes; D016376:Oligonucleotides, Antisense; D000509:alpha-Fetoproteins; D004247:DNA