title: The SUPERMAN gene family in Populus: nucleotide diversity and gene expression in a dioecious plant.
abstract: SUP gene family expression and regulation patterns reported in dioecious woody plant. Phylogenetic and nucleotide diversity analysis indicated PtoSUP1 is highly conserved and has undergone strong purifying selection. The molecular basis of SUPERMAN (SUP) regulation during floral development in monoecious plants has been extensively studied, but little is known of the SUP gene family in dioecious woody plants. In this study, we systematically examined the diversification of the SUP gene family in Populus, integrating genomic organization, expression, and phylogeny data. SUP family members showed sex-specific expression throughout flower development. Transcript profiling of rare gynomonoecious poplar flowers revealed that a significant reduction in PtoSUP1 mRNA might be important for stamen development in gynomonoecious poplar flowers. We found that the coding regions of Populus SUP genes are very highly conserved and that synonymous sites in exon regions have undergone strong purifying selection during SUP evolution in Populus. These results indicate that SUP genes play an important role in floral development of dioecious plants. Expression analysis of SUP suggested possible regulatory mechanisms for gynomonoecious poplar flower development. These findings provide an important insight into the mechanisms of the evolution of SUP function and may help enable engineered regulation of flower development for breeding improved tree varieties.
authors.affiliation: National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, Beijing, 100083, People's Republic of China. forward1985@163.com
authors.forename: Yuepeng
authors.initials: Y
authors.lastname: Song
authors.forename: Kaifeng
authors.initials: K
authors.lastname: Ma
authors.forename: Dong
authors.initials: D
authors.lastname: Ci
authors.forename: Xueyuan
authors.initials: X
authors.lastname: Tian
authors.forename: Zhiyi
authors.initials: Z
authors.lastname: Zhang
authors.forename: Deqiang
authors.initials: D
authors.lastname: Zhang
mesh_terms: D002980:Climate; D003062:Codon / Q000235:genetics; D035264:Flowers / Q000235:genetics; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics*; D014644:Genetic Variation; D008969:Molecular Sequence Data; D005810:Multigene Family; D009154:Mutation / Q000235:genetics; D009711:Nucleotides / Q000235:genetics*; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D032107:Populus / Q000235:genetics* / Q000254:growth & development; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D013045:Species Specificity
chemical_list: D003062:Codon; D009711:Nucleotides; D010937:Plant Growth Regulators; D010940:Plant Proteins; D012333:RNA, Messenger