title: Analysis of processing contaminants in edible oils. Part 2. Liquid chromatography-tandem mass spectrometry method for the direct detection of 3-monochloropropanediol and 2-monochloropropanediol diesters.
abstract: A method was developed and validated for the detection of fatty acid diesters of 2-monochloropropanediol (2-MCPD) and 3-monochloropropanediol (3-MCPD) in edible oils. These analytes are potentially carcinogenic chemical contaminants formed during edible oil processing. After separation from oil matrices using a two-step solid-phase extraction (SPE) procedure, the target compounds are quantitated using liquid chromatography-tandem mass spectrometry (LC-MS/MS) with electrospray ionization (ESI). The first chromatographic conditions have been developed that separate intact diesters of 2-MCPD and 3-MCPD, allowing for their individual quantitation. The method has been validated for 28 3-MCPD diesters of lauric, myristic, palmitic, linolenic, linoleic, oleic, and stearic acids in coconut, olive, and palm oils, as well as 3 2-MCPD diesters, using an external calibration curve. The range of average recoveries and relative standard deviations (RSDs) across the three oil matrices at three spiking concentrations are 88-118% (2-16% RSD) with maximum limits of quantitation of 30 ng/g (ppb).
authors.affiliation: Center for Food Safety and Applied Nutrition, United States Food and Drug Administration, College Park, MD 20740, USA. shaun.macmahon@fda.hhs.gov
authors.forename: Shaun
authors.initials: S
authors.lastname: MacMahon
authors.forename: Timothy H
authors.initials: TH
authors.lastname: Begley
authors.forename: Gregory W
authors.initials: GW
authors.lastname: Diachenko
mesh_terms: D002273:Carcinogens / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods*; D000074263:Coconut Oil; D004952:Esters / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005506:Food Contamination / Q000032:analysis*; D005990:Glycerol / Q000031:analogs & derivatives* / Q000032:analysis; D000069463:Olive Oil; D000073878:Palm Oil; D010938:Plant Oils / Q000032:analysis* / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000517:alpha-Chlorohydrin; D011409:Propylene Glycols
chemical_list: D002273:Carcinogens; D000074263:Coconut Oil; D004952:Esters; D005227:Fatty Acids; D005990:Glycerol; D000069463:Olive Oil; D000073878:Palm Oil; D010938:Plant Oils; D000517:alpha-Chlorohydrin; C585004:monochloropropanediol; D011409:Propylene Glycols