title: Flow cytometry evidence of human granulocytes interaction with polyhedral oligomeric silsesquioxanes: effect of nanoparticle charge.
abstract: Nanoparticles (NPs) entering the human body are immediately confronted with the innate part of human immune system. In particular, monocyte and neutrophil granulocytes readily clear particles by phagocytosis, even if in the case of NPs the uptake mechanism may be classified as macropinocytosis. Among engineered nanoparticles, in the last years, siliceous materials have emerged as promising materials for several applications ranging from catalysis to biomedical. The polyhedral oligomeric silsesquioxanes (POSS) are nanodimensional, easily synthesizable molecular compounds and POSS-based systems are promising carriers for biological molecules. In this work, the ability of human granulocytes to uptake positively and negatively charged POSS was measured using a simple flow cytometry analysis based on cell size modifications. The data obtained showed that after a 30 min exposure only positive NPs were uptaken by human granulocyte using both macropinocytosis and clathrin-mediated mechanisms as demonstrated by uptake inhibition mediated by amiloride and chlorpromazine.
authors.affiliation: Innovative Research Laboratory for Wound Healing, Health Sciences Department, University of Eastern Piedmont A. Avogadro, via Solaroli, 17 I-28100 Novara, Italy. filippo.reno@med.unipmn.it
authors.forename: Filippo
authors.initials: F
authors.lastname: Renò
authors.forename: Fabio
authors.initials: F
authors.lastname: Carniato
authors.forename: Manuela
authors.initials: M
authors.lastname: Rizzi
authors.forename: Francesco
authors.initials: F
authors.lastname: Olivero
authors.forename: Pamela
authors.initials: P
authors.lastname: Pittarella
authors.forename: Leonardo
authors.initials: L
authors.lastname: Marchese
mesh_terms: D000328:Adult; D002450:Cell Communication / Q000187:drug effects*; D016923:Cell Death / Q000187:drug effects; D005434:Flow Cytometry / Q000379:methods*; D006098:Granulocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D017646:Organosilicon Compounds / Q000494:pharmacology*; D055815:Young Adult
chemical_list: D017646:Organosilicon Compounds; C521417:polyhedraloligosilsesquioxane