title: Molecular characterization of the chicken anaemia viruses isolated from broiler farms of west Azerbaijan, Iran.
abstract: Chicken anaemia virus (CAV) is an economically important pathogen of chickens with worldwide distribution. CAV is the causative agent of chicken anaemia disease, causing severe anaemia, lymphoid atrophy and immunosuppression in young birds. In the present study, the genetic variation of circulating CAVs in west Azerbaijan broiler farms was investigated and compared with CAVs from other countries. Extracted viral DNA from livers of chickens positive for CAV (46 out of 100) was used and a fragment of the VP1 gene 1390 base pairs in size was amplified. The purified products were subjected to restriction fragment-length polymorphism (RFLP) using HinfI endonuclease and nucleotide sequencing. Four different RFLP patterns were identified from all examined CAV DNAs. Sequence analysis of the VP1 gene of isolated CAV viruses revealed a high genetic distance (0.5 to 4.7%) among CAV isolates. Phylogenetic analysis showed that CAVs isolated from Iranian poultry farms clustered with CAVs isolated from different parts of the world. It was concluded that the circulating CAVs in broiler farms of west Azerbaijan had a high genomic variation.
authors.affiliation: Department of Food Hygiene and Quality Control, Division of Molecular Epidemiology, Faculty of Veterinary Medicine, Urmia University, Urmia, West Azerbaijan, Iran.
authors.forename: Hadi
authors.initials: H
authors.lastname: Nayabian
authors.forename: Karim
authors.initials: K
authors.lastname: Mardani
mesh_terms: D000818:Animals; D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics; D017727:Chicken anemia virus / Q000235:genetics*; D002645:Chickens / Q000821:virology*; D017931:DNA Primers / Q000235:genetics; D014644:Genetic Variation; D007492:Iran; D016013:Likelihood Functions; D008099:Liver / Q000821:virology; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000662:veterinary
chemical_list: D036022:Capsid Proteins; D017931:DNA Primers; C442548:VP1 protein, Chicken anemia virus