title: Specific serology for emerging human coronaviruses by protein microarray.
abstract: We present a serological assay for the specific detection of IgM and IgG antibodies against the emerging human coronavirus hCoV-EMC and the SARS-CoV based on protein microarray technology. The assay uses the S1 receptor-binding subunit of the spike protein of hCoV-EMC and SARS-CoV as antigens. The assay has been validated extensively using putative cross-reacting sera of patient cohorts exposed to the four common hCoVs and sera from convalescent patients infected with hCoV-EMC or SARS-CoV.
authors.affiliation: Centre for Infectious Disease Control, Division Virology, National Institute for Public Health and the Environment, Bilthoven, The Netherlands. chantal.reusken@rivm.nl
authors.forename: C
authors.initials: C
authors.lastname: Reusken
authors.forename: H
authors.initials: H
authors.lastname: Mou
authors.forename: G J
authors.initials: GJ
authors.lastname: Godeke
authors.forename: L
authors.initials: L
authors.lastname: van der Hoek
authors.forename: B
authors.initials: B
authors.lastname: Meyer
authors.forename: M A
authors.initials: MA
authors.lastname: Müller
authors.forename: B
authors.initials: B
authors.lastname: Haagmans
authors.forename: R
authors.initials: R
authors.lastname: de Sousa
authors.forename: N
authors.initials: N
authors.lastname: Schuurman
authors.forename: U
authors.initials: U
authors.lastname: Dittmer
authors.forename: P
authors.initials: P
authors.lastname: Rottier
authors.forename: A
authors.initials: A
authors.lastname: Osterhaus
authors.forename: C
authors.initials: C
authors.lastname: Drosten
authors.forename: B J
authors.initials: BJ
authors.lastname: Bosch
authors.forename: M
authors.initials: M
authors.lastname: Koopmans
mesh_terms: D017934:Coronavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D018352:Coronavirus Infections / Q000097:blood / Q000469:parasitology; D005260:Female; D006801:Humans; D008297:Male; D040081:Protein Array Analysis; D017386:Sequence Homology, Amino Acid