title: Dimethylsulfoxide and conjugated linoleic acids affect bovine embryo development in vitro.
abstract: Conjugated linoleic acids (CLA) are employed to overcome the bovine periparturitional negative energy balance. Especially of interest are trans10,cis12 -linoleic acid (t10c12-CLA) and cis9,trans11-linoleic acid (c9t11-CLA). Their impact on embryonic development, though, is not clear. Here, effects of both above-mentioned CLA on bovine in vitro-produced embryos were assessed. Zygotes (n=2098) were allocated to one of seven groups: cultured with 50 or 100µM of either c9t11-CLA or t10c12-CLA, with 14 or 28mM DMSO or without supplement (control). Messenger RNA analysis of target gene transcripts (IGF1R, IGFBP2, IGFBP4, CPT2, ACAA1, ACAA2, FASN, SCD) via RT-qPCR was performed in single blastocysts. Cleavage rates did not differ, whereas development rates were decreased in both t10c12-supplemented groups in comparison to the unsupplemented group (31.7% ±2.2 control vs 20.2% ±2.0 50µM t10c12 vs 21.0% ±2.8 100µM t10c12). Compared with the unsupplemented group, SCD was expressed at a lower level in embryos cultured with 50µM c9t11-CLA. The relative amount of several transcripts was increased in embryos cultured with 14mM DMSO in comparison to those that developed in the presence of 50µM t10c12-CLA (IGFBP2, ACAA1, CPT2, FASN, SCD) or 50µM c9t11-CLA (IGF1R, IGFBP2, ACAA1, CPT2, FASN, SCD). The molecular analyses show that CLA influence embryonic fat metabolism.
authors.affiliation: Clinic for Cattle, University of Veterinary Medicine, Hannover, Foundation, Bischofsholer Damm 15, 30173 Hannover, Germany.
authors.forename: Hanna
authors.initials: H
authors.lastname: Stinshoff
authors.affiliation: Clinic for Cattle, University of Veterinary Medicine, Hannover, Foundation, Bischofsholer Damm 15, 30173 Hannover, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Wilkening
authors.affiliation: Clinic for Cattle, University of Veterinary Medicine, Hannover, Foundation, Bischofsholer Damm 15, 30173 Hannover, Germany.
authors.forename: Ana
authors.initials: A
authors.lastname: Hanstedt
authors.affiliation: Clinic for Cattle, University of Veterinary Medicine, Hannover, Foundation, Bischofsholer Damm 15, 30173 Hannover, Germany.
authors.forename: Heinrich
authors.initials: H
authors.lastname: Bollwein
authors.affiliation: Clinic for Cattle, University of Veterinary Medicine, Hannover, Foundation, Bischofsholer Damm 15, 30173 Hannover, Germany.
authors.forename: Christine
authors.initials: C
authors.lastname: Wrenzycki
mesh_terms: D000818:Animals; D002417:Cattle; D019587:Dietary Supplements; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D046149:Embryo Culture Techniques / Q000662:veterinary; D047108:Embryonic Development / Q000187:drug effects; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D044243:Linoleic Acids, Conjugated / Q000494:pharmacology*; D050356:Lipid Metabolism / Q000187:drug effects / Q000235:genetics; D011247:Pregnancy; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors; D015053:Zygote / Q000187:drug effects* / Q000378:metabolism
chemical_list: D044243:Linoleic Acids, Conjugated; D012333:RNA, Messenger; C503589:cis-9, trans-11-conjugated linoleic acid; C496197:trans-10,cis-12-conjugated linoleic acid; D004121:Dimethyl Sulfoxide