title: Comparative gene expression analysis of the human periodontal ligament in deciduous and permanent teeth.
abstract: There are histological and functional differences between human deciduous and permanent periodontal ligament (PDL) tissues. The aim of this study was to determine the differences between these two types of tissue at the molecular level by comparing their gene expression patterns. PDL samples were obtained from permanent premolars (n = 38) and anterior deciduous teeth (n = 31) extracted from 40 healthy persons. Comparative cDNA microarray analysis revealed several differences in gene expression between the deciduous and permanent PDL tissues. These findings were verified by qRT-PCR (quantitative reverse-transcription-polymerase chain reaction) analysis, and the areas where genes are expressed were revealed by immunohistochemical staining. The expressions of 21 genes were up-regulated in deciduous relative to PDL tissues, and those of 30 genes were up-regulated in permanent relative to deciduous PDL tissues. The genes that were up-regulated in deciduous PDL tissues were those involved in the formation of the extracellular matrix (LAMC2, LAMB3, and COMP), tissue development (IGF2BP, MAB21L2, and PAX3), and inflammatory or immune reactions leading to tissue degradation (IL1A, CCL21, and CCL18). The up-regulated genes in permanent PDL tissues were related to tissue degradation (IL6 and ADAMTS18), myocontraction (PDE3B, CASQ2, and MYH10), and neurological responses (FOS, NCAM2, SYT1, SLC22A3, DOCK3, LRRTM1, LRRTM3, PRSS12, and ARPP21). The analysis of differential gene expressions between deciduous and permanent PDL tissues aids our understanding of histological and functional differences between them at the molecular level.
authors.affiliation: Department of Pediatric Dentistry, College of Dentistry, Yonsei University, Seoul, Korea.
authors.forename: Je Seon
authors.initials: JS
authors.lastname: Song
authors.forename: Dong Hwan
authors.initials: DH
authors.lastname: Hwang
authors.forename: Seong-Oh
authors.initials: SO
authors.lastname: Kim
authors.forename: Mijeong
authors.initials: M
authors.lastname: Jeon
authors.forename: Byung-Jai
authors.initials: BJ
authors.lastname: Choi
authors.forename: Han-Sung
authors.initials: HS
authors.lastname: Jung
authors.forename: Seok Jun
authors.initials: SJ
authors.lastname: Moon
authors.forename: Wonse
authors.initials: W
authors.lastname: Park
authors.forename: Hyung-Jun
authors.initials: HJ
authors.lastname: Choi
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D010513:Periodontal Ligament / Q000166:cytology / Q000378:metabolism*; D014094:Tooth, Deciduous / Q000166:cytology / Q000378:metabolism*; D055815:Young Adult