title: Neuronal cell patterning on a multi-electrode array for a network analysis platform.
abstract: We studied neuronal cell patterning on a commercial multi-electrode array (MEA). We investigated the surface chemical modification of MEA in order to immobilize Poly-D-lysine (PDL) and then to pattern PDL with a photolithographic method using vacuum ultraviolet light (VUV). We have clarified that the PDL layer was not fully decomposed but was partially fragmented by short-time irradiation with VUV, resulting in a change in the cell adhesiveness of the PDL. We succeeded in patterning primary rat cortex cells without manipulating the cells on MEA more than two months. This cell-adhesiveness change induced by VUV can be applied to any immobilized PDL on other kinds of MEA and culturing substrate. We conducted electrophysiological measurements and found that the patterned neuronal cells were sufficiently matured and developed neural networks, demonstrating that our patterning method is useful for a neuronal network analysis platform.
authors.affiliation: National Institute of Advanced Industrial Science and Technology (AIST), Toyohira-ku, Sapporo 062-8517, Japan. suzuki-masaaki@aist.go.jp
authors.forename: Masaaki
authors.initials: M
authors.lastname: Suzuki
authors.forename: Koji
authors.initials: K
authors.lastname: Ikeda
authors.forename: Munehiro
authors.initials: M
authors.lastname: Yamaguchi
authors.forename: Suguru N
authors.initials: SN
authors.lastname: Kudoh
authors.forename: Keiko
authors.initials: K
authors.lastname: Yokoyama
authors.forename: Ryota
authors.initials: R
authors.lastname: Satoh
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ito
authors.forename: Masafumi
authors.initials: M
authors.lastname: Nagayama
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Uchida
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Gohara
mesh_terms: D000200:Action Potentials; D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods*; D049109:Cell Proliferation; D002478:Cells, Cultured; D004566:Electrodes; D006863:Hydrogen-Ion Concentration; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000166:cytology*; D056951:Photoelectron Spectroscopy; D051381:Rats; D012964:Sodium / Q000032:analysis; D055672:Static Electricity; D013499:Surface Properties
chemical_list: D012964:Sodium