title: [Development of a software standardizing optical density with operation settings related to several limitations].
abstract: OBJECTIVE
  To develop a software that can be used to standardize optical density to normalize the procedures and results of standardization in order to effectively solve several problems generated during standardization of in-direct ELISA results.
  
  
  METHODS
  The software was designed based on the I-STOD method with operation settings to solve the problems that one might encounter during the standardization. Matlab GUI was used as a tool for the development. The software was tested with the results of the detection of sera of persons from schistosomiasis japonica endemic areas.
  
  
  RESULTS
  I-STOD V1.0 (WINDOWS XP/WIN 7, 0.5 GB) was successfully developed to standardize optical density. A serial of serum samples from schistosomiasis japonica endemic areas were used to examine the operational effects of I-STOD V1.0 software. The results indicated that the software successfully overcame several problems including reliability of standard curve, applicable scope of samples and determination of dilution for samples outside the scope, so that I-STOD was performed more conveniently and the results of standardization were more consistent.
  
  
  CONCLUSIONS
  I-STOD V1.0 is a professional software based on I-STOD. It can be easily operated and can effectively standardize the testing results of in-direct ELISA.
authors.affiliation: School of Basic Medical Sciences, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Tu
authors.forename: Zuo-Heng
authors.initials: ZH
authors.lastname: Zhang
authors.forename: Cheng
authors.initials: C
authors.lastname: Wan
authors.forename: Yu
authors.initials: Y
authors.lastname: Zheng
authors.forename: Jin-Mei
authors.initials: JM
authors.lastname: Xu
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Zhang
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Luo
authors.forename: Hai-Wei
authors.initials: HW
authors.lastname: Wu
mesh_terms: D055620:Optical Phenomena; D012984:Software / Q000592:standards*