title: Illuminating cancer systems with genetically engineered mouse models and coupled luciferase reporters in vivo.
abstract: Bioluminescent imaging (BLI) is a powerful noninvasive tool that has dramatically accelerated the in vivo interrogation of cancer systems and longitudinal analysis of mouse models of cancer over the past decade. Various luciferase enzymes have been genetically engineered into mouse models (GEMM) of cancer, which permit investigation of cellular and molecular events associated with oncogenic transcription, posttranslational processing, protein-protein interactions, transformation, and oncogene addiction in live cells and animals. Luciferase-coupled GEMMs ultimately serve as a noninvasive, repetitive, longitudinal, and physiologic means by which cancer systems and therapeutic responses can be investigated accurately within the autochthonous context of a living animal.
authors.affiliation: Washington University School of Medicine, Campus Box 8225, 510 S. Kingshighway Boulevard, Box 8225, St. Louis, MO 63110, USA.
authors.forename: Brandon
authors.initials: B
authors.lastname: Kocher
authors.forename: David
authors.initials: D
authors.lastname: Piwnica-Worms
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005818:Genetic Engineering; D008156:Luciferases / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D008163:Luminescent Measurements / Q000379:methods; D051379:Mice; D009369:Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism
chemical_list: D008156:Luciferases