title: [Field evaluation of IgG antibody detection kits of Schistosoma japonicum and quality control analysis].
abstract: OBJECTIVE
  To evaluate the application effect of an ELISA kit for detection of IgG antibody to Schistosomajaponicum in endemic areas.
  
  
  METHODS
  In two schistosomiasis endemic villages of Eryuan County, Yunnan Province, there were 505 permanent residents enrolled in the study. Fecal examinations were carried out by using the nylon screen bag-egg concentration method. In paralleled testing, the ELISA kit was used for the villagers to detect the IgG antibody to S. japonicum. The assay was performed daily for 20 consecutive days, and the data were analyzed by comparison with the parasitological examination and by using the L - J quality control chart method.
  
  
  RESULTS
  In 505 examinees, 290 were positive by ELISA, with a positive rate of 57.43%. The L-J chart analysis showed that the deviation of A450 value between the quality control sera A and B was within the controllable range. The fecal examination found 20 positives with a positive rate 3.96%. Compared with the fecal examination, the positive consistency rate of the ELISA method reached to 90.00%.
  
  
  CONCLUSIONS
  The ELISA kit for detection of IgG antibody to S. japonicum is stable, reliable and suitable for the screening of schistosomiasis in the field.
authors.affiliation: National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Key Laboratory of Parasite and Vector Biology, Ministry of Health, WHO Collaborating Center for Malaria, Schistosomiasis and Filariasis, Shanghai 200025, China.
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Chu
authors.forename: Lin
authors.initials: L
authors.lastname: Ai
authors.forename: Jun-Min
authors.initials: JM
authors.lastname: Yao
authors.forename: Jian
authors.initials: J
authors.lastname: Guo
authors.forename: Yu-Chun
authors.initials: YC
authors.lastname: Cai
authors.forename: Jia-Xu
authors.initials: JX
authors.lastname: Chen
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology; D011786:Quality Control; D011933:Reagent Kits, Diagnostic; D012549:Schistosoma japonicum / Q000737:chemistry* / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D007074:Immunoglobulin G; D011933:Reagent Kits, Diagnostic