title: Single bout short duration fluid shear stress induces osteogenic differentiation of MC3T3-E1 cells via integrin β1 and BMP2 signaling cross-talk.
abstract: Fluid shear stress plays an important role in bone osteogenic differentiation. It is traditionally believed that pulsed and continuous stress load is more favorable for fracture recovery and bone homeostasis. However, according to our clinical practice, we notice that one single stress load is also sufficient to trigger osteogenic differentiation. In the present study, we subject osteoblast MC3T3-E1 cells to single bout short duration fluid shear stress by using a parallel plate flow system. The results show that 1 hour of fluid shear stress at 12 dyn/cm(2) promotes terminal osteogenic differentiation, including rearrangement of F-actin stress fiber, up-regulation of osteogenic genes expression, elevation of alkaline phosphatase activity, secretion of type I collagen and osteoid nodule formation. Moreover, collaboration of BMP2 and integrin β1 pathways plays a significant role in such differentiation processes. Our findings provide further experimental evidence to support the notion that single bout short duration fluid shear stress can promote osteogenic differentiation.
authors.affiliation: Department of Stomatology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, PR China.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Mai
authors.forename: Zhuli
authors.initials: Z
authors.lastname: Peng
authors.forename: Sihan
authors.initials: S
authors.lastname: Wu
authors.forename: Jinglan
authors.initials: J
authors.lastname: Zhang
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.forename: Huangyou
authors.initials: H
authors.lastname: Liang
authors.forename: Ding
authors.initials: D
authors.lastname: Bai
authors.forename: Guangmei
authors.initials: G
authors.lastname: Yan
authors.forename: Hong
authors.initials: H
authors.lastname: Ai
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D000880:Anthraquinones; D055396:Bone Morphogenetic Protein 2 / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D057446:Hydrodynamics; D019012:Integrin beta1 / Q000378:metabolism*; D051379:Mice; D018613:Microscopy, Confocal; D010012:Osteogenesis / Q000502:physiology*; D034622:RNA Interference; D060888:Real-Time Polymerase Chain Reaction; D020239:Receptor Cross-Talk / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D033081:Shear Strength / Q000502:physiology; D015398:Signal Transduction / Q000502:physiology*; D013314:Stress, Mechanical; D013997:Time Factors
chemical_list: D000880:Anthraquinones; C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D019012:Integrin beta1; C004468:Alizarin Red S; D000469:Alkaline Phosphatase