title: ¹H, ¹³C and ¹⁵N chemical shift assignments for the N-terminal PAS domain of the KCNH channel from zebrafish.
abstract: The KCNH channels are voltage-gated potassium channels that play important roles in heart and nerve cells. The N-terminal region of the KCNH channel contains a Per-Arnt-Sim (PAS) domain which is important for the channel gating through interaction with other regions of the channel. To study the solution structure of the N-terminal PAS domain of the KCNH channel from Zebrafish (zNTD), we over-expressed and purified zNTD. We report the resonance assignments for zNTD. The data will allow us to perform structural studies for this domain, which will provide insight into its structural basis for the molecular interaction with other regions of the KCNH channel.
authors.affiliation: Experimental Therapeutics Centre, Agency for Science, Technology and Research, 31 Biopolis Way Nanos, #03-01, Singapore, 138669, Singapore.
authors.forename: Young Mee
authors.initials: YM
authors.lastname: Kim
authors.forename: Qingxin
authors.initials: Q
authors.lastname: Li
authors.forename: Hui Qi
authors.initials: HQ
authors.lastname: Ng
authors.forename: Ho Sup
authors.initials: HS
authors.lastname: Yoon
authors.forename: CongBao
authors.initials: C
authors.lastname: Kang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002247:Carbon Isotopes; D051638:Ether-A-Go-Go Potassium Channels / Q000737:chemistry*; D006859:Hydrogen; D008969:Molecular Sequence Data; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D015027:Zebrafish / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000737:chemistry*
chemical_list: D002247:Carbon Isotopes; D051638:Ether-A-Go-Go Potassium Channels; D009587:Nitrogen Isotopes; D029961:Zebrafish Proteins; D006859:Hydrogen