title: Matrix-assisted laser desorption ionization-time of flight mass spectrometry in clinical microbiology.
abstract: Despite widespread application of nucleic acid diagnostics, cultures remain integral in modern laboratories. Because cultures detect a large number of organism types, it is unlikely that they will disappear from clinical practice in the near future. Their downside is slow turn-around time, impacted by time to growth and identification of that growth. The latter is expedited using a new proteomic technology, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS).
authors.affiliation: Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology and Division of Infectious Diseases, Department of Medicine, Mayo Clinic, Rochester, MN 55905, USA. patel.robin@mayo.edu
authors.forename: Robin
authors.initials: R
authors.lastname: Patel
mesh_terms: D001419:Bacteria / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification*; D001424:Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D005658:Fungi / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification*; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods*; D009181:Mycoses / Q000175:diagnosis* / Q000382:microbiology; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
keywords: MALDI-TOF; bacteria; fungi; mass spectrometry; matrix-assisted laser desorption ionization-time of flight