title: Immobilization of β-glucosidase on bifunctional periodic mesoporous organosilicas.
abstract: The aminopropyl-functionalized ethane-bridged bifunctional periodic mesoporous organosilicas (APEPMOs) were synthesized by the co-condensation of 1,2-bis (triethoxysilyl) ethane and 3-aminopropyltriethoxysilane in the presence of cationic surfactants octadecyltrimethylammonium chloride in basic medium. The pores of the APEPMOs were expanded with N,N-dimethyldecylamine and the pore-expanded materials were utilized as supports for β-glucosidase immobilization. A high enzyme loading of 120 mg per g support was achieved in 18 h, and 95.5 % of enzymatic activity was retained. β-Glucosidases were strongly immobilized on APEPMOs with only 5 % desorption in the washing step with buffer solution. The immobilized enzyme had 75 % activity after 20 batch reactions and had improved thermal stability, relative to the free enzyme. These results demonstrate that APEPMOs would be promising supports for β-glucosidases immobilization.
authors.affiliation: Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing, 210009, China.
authors.forename: Liye
authors.initials: L
authors.lastname: Guan
authors.forename: Bin
authors.initials: B
authors.lastname: Di
authors.forename: Mengxiang
authors.initials: M
authors.lastname: Su
authors.forename: Jie
authors.initials: J
authors.lastname: Qian
mesh_terms: D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D005959:Glucosidases / Q000737:chemistry / Q000378:metabolism*; D017646:Organosilicon Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D013696:Temperature
chemical_list: D004800:Enzymes, Immobilized; D017646:Organosilicon Compounds; D005959:Glucosidases