title: Electrospun bio-nanocomposite scaffolds for bone tissue engineering by cellulose nanocrystals reinforcing maleic anhydride grafted PLA.
abstract: Electrospun fibrous bio-nanocomposite scaffolds reinforced with cellulose nanocrystals (CNCs) were fabricated by using maleic anhydride (MAH) grafted poly(lactic acid) (PLA) as matrix with improved interfacial adhesion between the two components. Morphological, thermal, mechanical, and in vitro degradation properties as well as basic cytocompatibility using human adult adipose derived mesenchymal stem cells (hASCs) of MAH grafted PLA/CNC (i.e., MPLA/CNC) scaffolds were characterized. Morphological investigation indicated that the diameter and polydispersity of electrospun MPLA/CNC nanofibers were reduced with the increased CNC content. The addition of CNCs improved both the thermal stability and mechanical properties of MPLA/CNC composites. The MPLA/CNC scaffolds at the 5 wt % CNC loading level showed not only superior tensile strength (more than 10 MPa), but also improved stability during in vitro degradation compared with the MPLA and PLA/CNC counterparts. Moreover, the fibrous MPLA/CNC composite scaffolds were non-toxic to hASCs and capable of supporting cell proliferation. This study demonstrates that fibrous MPLA/CNC bio-nanocomposite scaffolds are biodegradable, cytocompatible, and possess useful mechanical properties for bone tissue engineering.
authors.affiliation: School of Renewable Natural Resources, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70803, United States.
authors.forename: Chengjun
authors.initials: C
authors.lastname: Zhou
authors.forename: Qingfeng
authors.initials: Q
authors.lastname: Shi
authors.forename: Weihong
authors.initials: W
authors.lastname: Guo
authors.forename: Lekeith
authors.initials: L
authors.lastname: Terrell
authors.forename: Ammar T
authors.initials: AT
authors.lastname: Qureshi
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Hayes
authors.forename: Qinglin
authors.initials: Q
authors.lastname: Wu
mesh_terms: D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D002482:Cellulose / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D008299:Maleic Anhydrides / Q000737:chemistry; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D013696:Temperature; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D008299:Maleic Anhydrides; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D002482:Cellulose