title: Effects of dppa3 on DNA methylation dynamics during primordial germ cell development in mice.
abstract: DNA methylation is a central epigenetic event that regulates cellular differentiation, reprogramming, and pathogenesis. Genomewide DNA demethylation occurs in preimplantation embryos and in embryonic germ cell precursors called primordial germ cells (PGCs). We previously showed that Dppa3, also known as Stella and PGC7, protects the maternal genome from tet methylcytosine dioxygenase 3 (Tet3)-mediated conversion of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) in zygotes. Here, we demonstrated that retrotransposon genes, such as long interspersed nuclear element-1 (Line-1) and intracisternal A particle (IAP), showed higher 5mC levels in Dppa3-null PGCs. In contrast, oxidative bisulfite sequence analysis revealed that the amounts of 5hmC in Line-1 and IAP were slightly reduced in the Dppa3-deficient PGCs. From our findings, we propose that Dppa3 is involved in the Tet-mediated active demethylation process during reprogramming of PGCs.
authors.affiliation: Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakashima
authors.forename: Tohru
authors.initials: T
authors.lastname: Kimura
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kaga
authors.forename: Tsunetoshi
authors.initials: T
authors.lastname: Nakatani
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Seki
authors.forename: Toshinobu
authors.initials: T
authors.lastname: Nakamura
authors.forename: Toru
authors.initials: T
authors.lastname: Nakano
mesh_terms: D000818:Animals; D002868:Chromosomal Proteins, Non-Histone; D019175:DNA Methylation; D047108:Embryonic Development / Q000235:genetics*; D044127:Epigenesis, Genetic; D018507:Gene Expression Regulation, Developmental; D007426:Genes, Intracisternal A-Particle / Q000235:genetics; D005854:Germ Cells / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D002868:Chromosomal Proteins, Non-Histone; C462180:Dppa3 protein, mouse; C561286:ECAT11 protein, mouse; D016601:RNA-Binding Proteins; D012097:Repressor Proteins
keywords: DNA demethylation; epigenetics; primordial germ cell