title: Glyceraldehyde-3-phosphate dehydrogenase, an immunogenic Streptococcus equi ssp. zooepidemicus adhesion protein and protective antigen.
abstract: Streptococcus equi ssp. zooepidemicus (Streptococcus zooepidemicus, SEZ) is an important pathogen associated with opportunistic infections of a wide range of species, including pigs and humans. The absence of a suitable vaccine makes it difficult to control SEZ infection. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has been previously identified as an immunogenic protein using immunoproteomic techniques. In the present study, we confirmed that the sequence of GAPDH was highly conserved with other Streptococcus spp. The purified recombinant GAPDH could elicit a significant humoral antibody response in mice and confer significant protection against challenge with a lethal dose of SEZ. GAPDH could adhere to the Hep-2 cells, confirmed by flow cytometry, and inhibit adherence of SEZ to Hep-2 cells in an adherence inhibition assay. In addition, real-time PCR demonstrated that GAPDH was induced in vivo following infection of mice with SEZ. These suggest that GAPDH could play an important role in the pathogenesis of SEZ infection and could be a target for vaccination against SEZ.
authors.affiliation: State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510006, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
authors.forename: Zigong
authors.initials: Z
authors.lastname: Wei
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Liu
authors.forename: Pingping
authors.initials: P
authors.lastname: Xiao
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Lu
authors.forename: Yaosheng
authors.initials: Y
authors.lastname: Chen
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001422:Bacterial Adhesion; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D002460:Cell Line; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism; D022781:Hepatocytes / Q000382:microbiology; D006801:Humans; D051379:Mice; D060888:Real-Time Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D013290:Streptococcal Infections / Q000276:immunology / Q000517:prevention & control*; D018502:Streptococcus equi / Q000201:enzymology* / Q000276:immunology*; D016019:Survival Analysis; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification
chemical_list: D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+)