title: Nucleus pulposus cells expressing hBMP7 can prevent the degeneration of allogenic IVD in a canine transplantation model.
abstract: We have previously explored the possibilities of allogenic intervertebral disc (IVD) curing disc degeneration disease in clinical practice. The results showed that the motion and stability of the spinal unit was preserved after transplantation of allogenic IVD in human beings at 5-year follow-up. However, mild degeneration was observed in the allogenic transplanted IVD cases. In this study, we construct the biological tissue engineering IVD by injecting the nucleus pulposus cells (NPCs) expressing human bone morphogenetic protein 7 (hBMP7) into cryopreserved IVD, and transplant the biological tissue engineering IVD into a beagle dog to investigate whether NPCs expressing hBMP7 could prevent the degeneration of the transplanted allogenic IVDs. At 24 weeks after transplantation, MRI scan showed that IVD allografts injected NPCs expressing hBMP7 have a slighter signs of degeneration than IVD allografts with NPCs or without NPCs. The range of motion of left-right rotation in the group without NPCs was bigger than that of two cells injection group. PKH-26-labeled cells were identified at IVD allograft. The study demonstrated that NPCs expressing hBMP7 could survive at least 24 weeks and prevent the degeneration of the transplanted IVD. This solution might have a potential role in preventing the IVD allograft degeneration in long time follow-up.
authors.affiliation: Department of Orthopedic Surgery, Navy General Hospital, Fucheng Road No. 6, Haidian District, Beijing, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Chaofeng
authors.forename: Zhang
authors.initials: Z
authors.lastname: Chao
authors.forename: Wang
authors.initials: W
authors.lastname: Deli
authors.forename: Wu
authors.initials: W
authors.lastname: Jianhong
authors.forename: Zhang
authors.initials: Z
authors.lastname: Yan
authors.forename: Xu
authors.initials: X
authors.lastname: Cheng
authors.forename: Xin
authors.initials: X
authors.lastname: Hongkui
authors.forename: He
authors.initials: H
authors.lastname: Qing
authors.forename: Ruan
authors.initials: R
authors.lastname: Dike
mesh_terms: D000818:Animals; D055419:Bone Morphogenetic Protein 7 / Q000235:genetics / Q000378:metabolism*; D015925:Cryopreservation; D004195:Disease Models, Animal; D004285:Dogs; D005786:Gene Expression Regulation; D007403:Intervertebral Disc / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D055959:Intervertebral Disc Degeneration / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*; D008279:Magnetic Resonance Imaging; D012333:RNA, Messenger / Q000378:metabolism; D016059:Range of Motion, Articular; D023822:Tissue Engineering; D014184:Transplantation, Homologous
chemical_list: C525819:BMP7 protein, human; D055419:Bone Morphogenetic Protein 7; D012333:RNA, Messenger
keywords: bone morphogenetic protein 7; degeneration of intervertebral disc; gene therapy; transfection; transplantation of intervertebral disc