title: Widening the pH activity profile of a fungal laccase by directed evolution.
abstract: Unnatural selection: A fungal laccase was tailored by directed evolution to be active at neutral/alkaline pH. After five generations, the final mutant showed a broader pH profile while retaining 50 to 80 % of its activity at neutral pH.
authors.affiliation: Department of Biocatalysis, Institute of Catalysis, CSIC, Marie Curie St. 2, 28049 Madrid, Spain.
authors.forename: Pamela
authors.initials: P
authors.lastname: Torres-Salas
authors.forename: Diana M
authors.initials: DM
authors.lastname: Mate
authors.forename: Iraj
authors.initials: I
authors.lastname: Ghazi
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Plou
authors.forename: Antonio O
authors.initials: AO
authors.lastname: Ballesteros
authors.forename: Miguel
authors.initials: M
authors.lastname: Alcalde
mesh_terms: D019020:Directed Molecular Evolution; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000235:genetics* / Q000378:metabolism*; D008958:Models, Molecular; D009154:Mutation; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D013379:Substrate Specificity
chemical_list: D042845:Laccase