title: Absence of Epstein-Barr and cytomegalovirus infection in neuroblastoma cells by standard detection methodologies.
abstract: Indications exist in the scientific literature that infection with human herpes family viruses may contribute to the pathogenesis of neuroblastoma (NB). However, systematic investigations regarding viral presence in NB cells have been scarcely reported. Here, the presence of DNA from Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV) was assessed by PCR in 12 NBs, supplemented with RNA in situ hybridization, immunohistochemical detection, and high-throughput DNA sequencing. These standard methods did not detect infection by EBV or HCMV in NB cells in any tumor, while occasional immune cells were positive for EBV RNA or HCMV protein in four cases.
authors.affiliation: Division of Clinical Genetics, Department of Laboratory Medicine, Lund University, Lund, Sweden.
authors.forename: Daniel
authors.initials: D
authors.lastname: Sehic
authors.forename: Ola
authors.initials: O
authors.lastname: Forslund
authors.forename: Emma
authors.initials: E
authors.lastname: Sandén
authors.forename: Linda Holmquist
authors.initials: LH
authors.lastname: Mengelbier
authors.forename: Jenny
authors.initials: J
authors.lastname: Karlsson
authors.forename: Davit
authors.initials: D
authors.lastname: Bzhalava
authors.forename: Johanna
authors.initials: J
authors.lastname: Ekström
authors.forename: Janina
authors.initials: J
authors.lastname: Warenholt
authors.forename: Anna
authors.initials: A
authors.lastname: Darabi
authors.forename: Joakim
authors.initials: J
authors.lastname: Dillner
authors.forename: Ingrid
authors.initials: I
authors.lastname: Øra
authors.forename: David
authors.initials: D
authors.lastname: Gisselsson
mesh_terms: D003587:Cytomegalovirus; D003586:Cytomegalovirus Infections; D020031:Epstein-Barr Virus Infections; D005260:Female; D004854:Herpesvirus 4, Human; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D009447:Neuroblastoma; D016133:Polymerase Chain Reaction; D012367:RNA, Viral
chemical_list: D012367:RNA, Viral
keywords: Epstein-Barr virus; cytomegalovirus; metagenomics; neuroblastoma