title: Mechanical control of cell flow in multicellular spheroids.
abstract: Collective cell motion is observed in a wide range of biological processes. In tumors, physiological gradients of nutrients, growth factors, or even oxygen give rise to gradients of proliferation. We show using fluorescently labeled particles that these gradients drive a velocity field resulting in a cellular flow in multicellular spheroids. Under mechanical stress, the cellular flow is drastically reduced. We describe the results with a hydrodynamic model that considers only convection of the particles by the cellular flow.
authors.affiliation: Physicochimie Curie (Institut Curie, CNRS-UMR168, UPMC), Centre de Recherche, 26 rue d'Ulm, 75248 Paris Cedex 05, France.
authors.forename: Morgan
authors.initials: M
authors.lastname: Delarue
authors.forename: Fabien
authors.initials: F
authors.lastname: Montel
authors.forename: Ouriel
authors.initials: O
authors.lastname: Caen
authors.forename: Jens
authors.initials: J
authors.lastname: Elgeti
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Siaugue
authors.forename: Danijela
authors.initials: D
authors.lastname: Vignjevic
authors.forename: Jacques
authors.initials: J
authors.lastname: Prost
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Joanny
authors.forename: Giovanni
authors.initials: G
authors.lastname: Cappello
mesh_terms: D000818:Animals; D017554:Carbon Compounds, Inorganic / Q000737:chemistry; D048708:Cell Growth Processes / Q000502:physiology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D003110:Colonic Neoplasms / Q000473:pathology; D003470:Culture Media; D003911:Dextrans / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D057446:Hydrodynamics; D051379:Mice; D008954:Models, Biological; D053758:Nanoparticles / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D018874:Spheroids, Cellular / Q000166:cytology*; D013314:Stress, Mechanical; D013440:Sulfides / Q000737:chemistry
chemical_list: D017554:Carbon Compounds, Inorganic; D003470:Culture Media; D003911:Dextrans; D005456:Fluorescent Dyes; D013440:Sulfides; C092035:carbon sulfide; D012822:Silicon Dioxide