title: Investigation of depth-resolved nanoscale structural changes in regulated cell proliferation and chromatin decondensation.
abstract: We present depth-resolved spatial-domain low-coherence quantitative phase microscopy, a simple approach that utilizes coherence gating to construct a depth-resolved structural feature vector quantifying sub-resolution axial structural changes at different optical depths within the sample. We show that this feature vector is independent of sample thickness variation, and identifies nanoscale structural changes in clinically prepared samples. We present numerical simulations and experimental validation to demonstrate the feasibility of the approach. We also perform experiments using unstained cells to investigate the nanoscale structural changes in regulated cell proliferation through cell cycle and chromatin decondensation induced by histone acetylation.
authors.affiliation: Biomedical and Optical Imaging Laboratory (BOIL), Departments of Medicine and Bioengineering, University of Pittsburgh, Pittsburgh, PA 15213, USA.
authors.forename: Shikhar
authors.initials: S
authors.lastname: Uttam
authors.forename: Rajan K
authors.initials: RK
authors.lastname: Bista
authors.forename: Kevin
authors.initials: K
authors.lastname: Staton
authors.forename: Sergey
authors.initials: S
authors.lastname: Alexandrov
authors.forename: Serah
authors.initials: S
authors.lastname: Choi
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Bakkenist
authors.forename: Douglas J
authors.initials: DJ
authors.lastname: Hartman
authors.forename: Randall E
authors.initials: RE
authors.lastname: Brand
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
keywords: (170.1610) Clinical applications; (170.4730) Optical pathology; (180.3170) Interference microscopy; (300.6300) Spectroscopy, Fourier transforms