title: Construction of photoenergetic mitochondria in cultured mammalian cells.
abstract: The proton motive force (PMF) is bio-energetically important for various cellular reactions to occur. We developed PMF-photogenerating mitochondria in cultured mammalian cells. An archaebacterial rhodopsin, delta-rhodopsin, which is a light-driven proton pump derived from Haloterrigena turkmenica, was expressed in the mitochondria of CHO-K1 cells. The constructed stable CHO-K1 cell lines showed suppression of cell death induced by rotenone, a pesticide that inhibits mitochondrial complex I activity involved in PMF generation through the electron transport chain. Delta-rhodopsin was also introduced into the mitochondria of human neuroblastoma SH-SY5Y cells. The constructed stable SH-SY5Y cell lines showed suppression of dopaminergic neuronal cell death induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), an inducer of Parkinson's disease models, which acts through inhibition of complex I activity. These results suggest that the light-activated proton pump functioned as a PMF generator in the mitochondria of mammalian cells, and suppressed cell death induced by inhibition of respiratory PMF generation.
authors.affiliation: Organization of Advanced Science and Technology, Kobe University, Tokyo, Japan.
authors.forename: Kiyotaka Y
authors.initials: KY
authors.lastname: Hara
authors.forename: Takeyoshi
authors.initials: T
authors.lastname: Wada
authors.forename: Kuniki
authors.initials: K
authors.lastname: Kino
authors.forename: Toru
authors.initials: T
authors.lastname: Asahi
authors.forename: Naoya
authors.initials: N
authors.lastname: Sawamura
mesh_terms: D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D003412:Cricetulus; D015870:Gene Expression; D006801:Humans; D008928:Mitochondria / Q000235:genetics / Q000378:metabolism*; D009447:Neuroblastoma / Q000378:metabolism; D018892:Proton-Motive Force / Q000502:physiology*; D006180:Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism
chemical_list: D006180:Proton-Translocating ATPases