title: Profiling glycosyltransferase activities by tritium imaging of glycan microarrays.
abstract: High-throughput microarray technology has been combined with ultrasensitive and high-resolution tritium autoradiography to create a new platform for the quantitative detection of glycosyltransferase activity on glycan arrays. In addition, we show full compatibility with the use of fluorescently labeled lectins to help with the stereochemical assignment of newly formed glycoside linkages.
authors.affiliation: Biofunctional Nanomaterials Unit, CIC biomaGUNE, P° de Miramón 182, Parque Tecnológico, 20009 San Sebastian, Spain.
authors.forename: Sonia
authors.initials: S
authors.lastname: Serna
authors.forename: Cornelis H
authors.initials: CH
authors.lastname: Hokke
authors.forename: Martin
authors.initials: M
authors.lastname: Weissenborn
authors.forename: Sabine
authors.initials: S
authors.lastname: Flitsch
authors.forename: Manuel
authors.initials: M
authors.lastname: Martin-Lomas
authors.forename: Niels-Christian
authors.initials: NC
authors.lastname: Reichardt
mesh_terms: D002236:Carbohydrate Conformation; D004789:Enzyme Activation; D016695:Glycosyltransferases / Q000032:analysis / Q000378:metabolism*; D046228:Microarray Analysis; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000378:metabolism*; D012555:Schistosomiasis mansoni / Q000201:enzymology; D014316:Tritium / Q000032:analysis* / Q000378:metabolism
chemical_list: D011134:Polysaccharides; D014316:Tritium; D016695:Glycosyltransferases