title: Tissue damage in organic rainbow trout muscle investigated by proteomics and bioinformatics.
abstract: The response to tissue damage is a complex process, which involves the coordinated regulation of multiple proteins to ensure tissue repair. In order to investigate the effect of tissue damage in a lower vertebrate, samples were taken from rainbow trout (Oncorhynchus mykiss) at day 7 after damage and proteins were separated using 2DE. The experimental design included two groups of rainbow trout, which were fed organic feed either with or without astaxanthin. In total, 96 proteins were found to be affected by tissue damage, clearly demonstrating in this lower vertebrate the complexity and magnitude of the cellular response, in the context of a regenerative process. Using a bioinformatics approach, the main biological function of these proteins were assigned, showing the regulation of proteins involved in processes such as apoptosis, iron homeostasis, and regulation of muscular structure. Interestingly, it was established that exclusively within the astaxanthin feed group, three members of the annexin protein family (annexin IV, V, and VI) were regulated in response to tissue damage.
authors.affiliation: DTU Food, National Food Institute, Technical University of Denmark, Søltofts Plads, Lyngby, Denmark. tuwu@food.dtu.dk
authors.forename: Tune
authors.initials: T
authors.lastname: Wulff
authors.forename: Tomé
authors.initials: T
authors.lastname: Silva
authors.forename: Michael Engelbrecht
authors.initials: ME
authors.lastname: Nielsen
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D016000:Cluster Analysis; D029941:Fish Proteins / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000293:injuries* / Q000378:metabolism*; D017686:Oncorhynchus mykiss / Q000293:injuries* / Q000378:metabolism*; D060066:Protein Interaction Maps; D020543:Proteome / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D011897:Random Allocation; D014945:Wound Healing; D024341:Xanthophylls / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D029941:Fish Proteins; D020543:Proteome; D024341:Xanthophylls; C005948:astaxanthine
keywords: Animal proteomics; Astaxanthin; Organic; Rainbow trout; Tissue damage; Wound repair