title: A direct fluorescence-based technique for cellular localization of amylin.
abstract: Amylin has been implicated in type II diabetes because of its inherent property to misfold into toxic aggregates. Although it has been shown that amylin interacts with cell membranes, no study to date has monitored the association process using a direct approach. The present study uses confocal microscopy to identify the localization of carboxyfluorescein-labeled amylin in RIN-5F cells. In addition, the size of the aggregates that are formed was evaluated using nanoparticle tracking analysis. In support of previous findings, amylin was observed to interact with and remain associated with the cell membrane. The cell membrane-associated aggregates spanned a size range of 130-800 nm.
authors.affiliation: School of Life Sciences, University of KwaZulu-Natal, South Africa.
authors.forename: Karen
authors.initials: K
authors.lastname: Pillay
authors.forename: Patrick
authors.initials: P
authors.lastname: Govender
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry; D006801:Humans; D058228:Islet Amyloid Polypeptide / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D021381:Protein Transport; D051381:Rats
chemical_list: D005456:Fluorescent Dyes; D058228:Islet Amyloid Polypeptide
keywords: RIN-5F; amylin; carboxyfluorescein; type II diabetes