title: Acetone, butanol, and ethanol production from cane molasses using Clostridium beijerinckii mutant obtained by combined low-energy ion beam implantation and N-methyl-N-nitro-N-nitrosoguanidine induction.
abstract: In order to obtain mutant strains showing higher solvent tolerance and butanol production than those of wild-type strains, the butanol-producing strain Clostridium beijerinckii L175 was subjected to mutagenesis using a combined method of low-energy ion beam implantation and N-methyl-N-nitro-N-nitrosoguanidine induction. With this effort, mutant strain MUT3 was isolated. When it was used for butanol fermentation in P2 medium, the production of butanol was 15.8±0.7 g/L 46% higher than the wild-type strain. Furthermore, after optimization of butanol production from cane molasses with MUT3, the maximum butanol production of 14.9±0.5 g/L were obtained in crew-capped bottles. When ABE production by MUT3 was carried out in a bioreactor, the production of butanol and total solvent were 15.1±0.8 g/L and 22.1±0.9 g/L, respectively. The remarkable butanol production and solvent tolerance of MUT3 make it promising for butanol production from cane molasses.
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
authors.forename: Han-guang
authors.initials: HG
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Luo
authors.forename: Qiu-ya
authors.initials: QY
authors.lastname: Gu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.forename: Wen-jun
authors.initials: WJ
authors.lastname: Hu
authors.forename: Xiao-bin
authors.initials: XB
authors.lastname: Yu
mesh_terms: D000096:Acetone / Q000378:metabolism*; D019149:Bioreactors; D000440:Butanols / Q000378:metabolism*; D046970:Clostridium beijerinckii / Q000235:genetics* / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D008965:Molasses; D016296:Mutagenesis; D009604:Nitrosoguanidines / Q000378:metabolism*
chemical_list: D000440:Butanols; D009604:Nitrosoguanidines; D000096:Acetone; D000431:Ethanol