title: Glycoproteomic analysis of tissues from patients with colon cancer using lectin microarrays and nanoLC-MS/MS.
abstract: The current study evaluated the glycoproteomic profile of tissues from colon cancer patients. The lectin microarray was first performed to compare the glycoprotein profiles between colon cancer and matched normal tissues. Level of N-acetylglucosamine (GlcNAc) that Solanum tuberosum lectin (STL) bound was found to be elevated in colon cancer, which was verified through lectin histochemistry. The subsequent glycoproteomic analysis based on STL enrichment of glycoproteins followed by label-free quantitative nano liquid chromatography-mass spectrometry/mass spectrometry (nanoLC-MS/MS) analysis identified 72 proteins in high confidence. Among these proteins, 17 were exclusively detected in cancer tissues, and 14 were significantly upregulated in tumor tissues. Annexin A1 and HSP90β were chosen for further investigation by immunoprecipitation coupled with lectin blots, western blots and tissue microarrays. Both Annexin A1 and HSP90β were GlcNAcylated, and their protein expressions were elevated in colon cancer, compared to normal tissues. Moreover, specific changes of GlcNAc abundances in Annexin A1 and HSP90β suggested that tumor-specific glycan patterns could serve as candidate biomarkers of colon cancer for distinguishing cancer patients from healthy individuals.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, 94 Weijin Road, Tianjin 300071, PR China.
authors.forename: Yangguang
authors.initials: Y
authors.lastname: Li
authors.forename: Ti
authors.initials: T
authors.lastname: Wen
authors.forename: Minzhi
authors.initials: M
authors.lastname: Zhu
authors.forename: Lixin
authors.initials: L
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Wei
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wu
authors.forename: Mingzhou
authors.initials: M
authors.lastname: Guo
authors.forename: Shuangping
authors.initials: S
authors.lastname: Liu
authors.forename: Huiyuan
authors.initials: H
authors.lastname: Zhao
authors.forename: Siyuan
authors.initials: S
authors.lastname: Xia
authors.forename: Weili
authors.initials: W
authors.lastname: Huang
authors.forename: Puyue
authors.initials: P
authors.lastname: Wang
authors.forename: Zhenzhou
authors.initials: Z
authors.lastname: Wu
authors.forename: Liqing
authors.initials: L
authors.lastname: Zhao
authors.forename: Wenqing
authors.initials: W
authors.lastname: Shui
authors.forename: Zheng
authors.initials: Z
authors.lastname: Li
authors.forename: Zhinan
authors.initials: Z
authors.lastname: Yin
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D003106:Colon / Q000378:metabolism; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006023:Glycoproteins / Q000032:analysis / Q000737:chemistry*; D006801:Humans; D037102:Lectins / Q000737:chemistry / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D040081:Protein Array Analysis; D020543:Proteome / Q000032:analysis / Q000737:chemistry*; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry
chemical_list: D006023:Glycoproteins; D037102:Lectins; D020543:Proteome