title: Association of eNOS gene intron 4 a/b VNTR polymorphisms in children with nephrotic syndrome.
abstract: To investigate the association of endothelial nitric oxide synthase gene intron 4 (eNOS4) polymorphisms with nephrotic syndrome, the eNOS4 genotypes were assessed in 161 children with nephrotic syndrome in comparison with 78 healthy subjects. We classified the children with nephritic syndrome into 2 groups: as steroid-sensitive nephrotic syndrome (SSNS) (n=125) and steroid-resistant nephrotic syndrome (SRNS) (n=36). The eNOS4 polymorphisms were analyzed by polymerase chain reaction. The frequencies of eNOS4 aa, ab and bb genotypes were 3%, 31%, and 66% in all the nephrotic syndrome groups, and 1%, 23%, and 76% in the control group (x(2)=2.87, p>0.05). In addition, the frequencies of eNOS4 aa, ab and bb genotypes were 2%, 33%, and 65% in SSNS group, and 5%, 28%, and 67% in the SRNS group (x(2)=1.13, p=0.567). The present study is the first to investigate eNOS4 gene polymorphisms in children with SSNS and SRNS. Our data show that the eNOS4 gene polymorphisms were not associated with the development, frequent relapse and response to steroid in nephritic syndrome.
authors.affiliation: Çukurova University School of Medicine, Department of Pediatric Nephrology, Adana, Turkey. dursunhs@yahoo.com
authors.forename: Hasan
authors.initials: H
authors.lastname: Dursun
authors.forename: Aytul
authors.initials: A
authors.lastname: Noyan
authors.forename: Selcuk
authors.initials: S
authors.lastname: Matyar
authors.forename: Mithat
authors.initials: M
authors.lastname: Buyukcelik
authors.forename: Mustafa
authors.initials: M
authors.lastname: Soran
authors.forename: Nurcan
authors.initials: N
authors.lastname: Cengiz
authors.forename: Aysun K
authors.initials: AK
authors.lastname: Bayazit
authors.forename: Gulsah
authors.initials: G
authors.lastname: Seydaoglu
authors.forename: Gulen
authors.initials: G
authors.lastname: Attila
authors.forename: Ali
authors.initials: A
authors.lastname: Anarat
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D018598:Minisatellite Repeats / Q000235:genetics*; D009404:Nephrotic Syndrome / Q000235:genetics*; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
chemical_list: C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III