title: [Whole-genome messenger RNA profiling reveals genes involved in malignant progression of glioma].
abstract: OBJECTIVE
  To employ whole-genome messenger RNA profiling to identify the genes involved in malignant progression in glioma.
  
  
  METHODS
  The whole genome expressed genes were profiled in 220 glioma patients from the Chinese Glioma Genome Atlas (97 LGGs and 123 HGGs). The differential expressed genes between LGG and HGG were identified by SAM analysis. Microarray data were validated by immunohistochemistry.
  
  
  RESULTS
  Among all the detected genes, the genes up-regulated mostly in high-grade glioma were IGFBP-2, CKLF, PTTG1, OSTCL and PTTG2 while those down-regulated mostly SEC31, RRP7B, HOOK3, SNRPN and CSMD3. Validation of IGFBP-2 with immunohistochemical staining showed a good correlation with the microarray data.
  
  
  CONCLUSIONS
  A panel of potential genes of malignant transformation may serve as future targets of gene therapy for glioma.
authors.affiliation: Department of Neurosurgery, Capital Medical University, Beijing, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Yan
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Gan
authors.initials: G
authors.lastname: You
authors.forename: Zhao-shi
authors.initials: ZS
authors.lastname: Bao
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
mesh_terms: D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology*; D020869:Gene Expression Profiling; D052138:Genes, Neoplasm; D005910:Glioma / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D012333:RNA, Messenger