title: Celecoxib inhibits Helicobacter pylori-induced invasion of gastric cancer cells through an adenine nucleotide translocator-dependent mechanism.
abstract: Cyclooxygenase-2 (COX-2) inhibitor, celecoxib, causes growth inhibition of human gastric carcinoma cells, but it remains unclear whether celecoxib inhibits Helicobacter pylori-induced invasion of gastric cancer cells. The adenine nucleotide translocator (ANT) is a mitochondrial bi-functional protein. We speculate that ANT-dependent pathways might contribute to H. pylori-induced invasion and metastasis of gastric cancer cells. Therefore, in the present study, we evaluate the effect of celecoxib on H. pylori-induced gastric cancer cell motility and invasion. We also explore the role of ANTs in H. pylori-induced gastric cancer cell motility and invasion of gastric cancer cell line AGS. Our results demonstrate that celecoxib induces anoikis-like apoptosis and suppresses the proliferation and invasion of gastric cancer cells induced by H. pylori in culture. RT-PCR and Western blot analysis indicates that celecoxib upregulates the expression of ANT1 and ANT3; however, celecoxib did not increase the expression of ANT2. Our results suggest that celecoxib could be an effective means for suppressing proliferation and invasion of gastric cancer cells induced by H. pylori through an adenine nucleotide translocator-dependent mechanism.
authors.affiliation: Department of Gastroenterology, Third Military Medical University, Chongqing 400038, China.
authors.forename: Chunhui
authors.initials: C
authors.lastname: Lan
authors.forename: Liuqin
authors.initials: L
authors.lastname: Yang
authors.forename: Lilin
authors.initials: L
authors.lastname: Fan
authors.forename: Yafei
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Guo Jun
authors.initials: GJ
authors.lastname: Guo
authors.forename: Shunmei
authors.initials: S
authors.lastname: Wan
authors.forename: Shiming
authors.initials: S
authors.lastname: Yang
authors.forename: Rongquan
authors.initials: R
authors.lastname: Wang
authors.forename: Dianchun
authors.initials: D
authors.lastname: Fang
mesh_terms: D023102:Anoikis / Q000187:drug effects; D000068579:Celecoxib; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D052246:Cyclooxygenase 2 Inhibitors / Q000494:pharmacology*; D016481:Helicobacter Infections / Q000150:complications / Q000378:metabolism / Q000473:pathology*; D016480:Helicobacter pylori / Q000502:physiology*; D006801:Humans; D000226:Mitochondrial ADP, ATP Translocases / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D020033:Protein Isoforms / Q000378:metabolism; D011720:Pyrazoles / Q000494:pharmacology*; D013274:Stomach Neoplasms / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D013449:Sulfonamides / Q000494:pharmacology*
chemical_list: D052246:Cyclooxygenase 2 Inhibitors; D020033:Protein Isoforms; D011720:Pyrazoles; D013449:Sulfonamides; D000226:Mitochondrial ADP, ATP Translocases; D000068579:Celecoxib