title: Electrophysiological evidence for 4-isobutyl-3-isopropylbicyclophosphorothionate as a selective blocker of insect GABA-gated chloride channels.
abstract: Invertebrate γ-aminobutyric acid (GABA)-gated chloride channels (GABACls) and glutamate-gated chloride channels (GluCls), which function as inhibitory neurotransmitter receptors, are important targets of insecticides and antiparasitic agents. The antagonism of GABACls and GluCls by 4-isobutyl-3-isopropylbicyclophosphorothionate (PS-14) was examined in cultured cockroach and rat neurons using a whole-cell patch-clamp method. The results indicated that PS-14 selectively blocks cockroach GABACls relative to cockroach GluCls and rat GABACls. PS-14 represents a useful probe for the study of insect GABA receptors.
authors.affiliation: Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, Matsue, Shimane 690-8504, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Akiyoshi
authors.forename: Xiu-Lian
authors.initials: XL
authors.lastname: Ju
authors.forename: Shogo
authors.initials: S
authors.lastname: Furutani
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Matsuda
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Ozoe
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018118:Chloride Channels / Q000037:antagonists & inhibitors* / Q000378:metabolism; D003058:Cockroaches / Q000187:drug effects / Q000378:metabolism*; D007313:Insecta / Q000378:metabolism*; D007306:Insecticides / Q000138:chemical synthesis / Q000737:chemistry* / Q000633:toxicity; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D018408:Patch-Clamp Techniques; D010710:Phosphates / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D011485:Protein Binding; D051381:Rats; D011963:Receptors, GABA-A / Q000737:chemistry / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000737:chemistry
chemical_list: D018118:Chloride Channels; D007306:Insecticides; D010710:Phosphates; D011963:Receptors, GABA-A; C109777:glutamate-gated chloride channels; D005680:gamma-Aminobutyric Acid; C083120:phosphorodithioic acid