title: Effects of hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 polymorphisms on fat androstenone level and gene expression in Duroc pigs.
abstract: A high level of androstenone in porcine adipose tissue is a major factor contributing to boar taint. Porcine hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (3β-HSD, also known as HSD3B1) plays a key role in the hepatic metabolism that catalyzes androstenone to β-androstenol. Therefore, 3β-HSD is a candidate gene for boar taint. This study aimed to investigate functional 3β-HSD polymorphisms in Duroc pigs. We found eight single nucleotide polymorphisms (SNPs) in the full-length porcine 3β-HSD. Four of the SNPs had restriction enzyme sites, and we genotyped them in 147 uncastrated male Duroc pigs using a polymerase chain reaction-restriction fragment length polymorphism method. Pigs with the GG genotype at the g.165262G>A locus (SNP5) had significantly lower androstenone levels than did those with other genotypes (P = 0.030). SNP5 also was associated with differences in 3β-HSD mRNA levels: pigs with the GG genotype had higher levels than those with other genotypes (P = 0.019). The SNP5 polymorphism could affect the hepatic catabolism of androstenone and consequently impact androstenone accumulation in the adipose tissue. Therefore, SNP5 in the 3β-HSD of Duroc pigs could be a useful selective marker for decreasing boar taint.
authors.affiliation: College of Life Sciences and Biotechnology, Korea University, Seoul, South Korea.
authors.forename: J M
authors.initials: JM
authors.lastname: Kim
authors.forename: J H
authors.initials: JH
authors.lastname: Ahn
authors.forename: K S
authors.initials: KS
authors.lastname: Lim
authors.forename: E A
authors.initials: EA
authors.lastname: Lee
authors.forename: T
authors.initials: T
authors.lastname: Chun
authors.forename: K C
authors.initials: KC
authors.lastname: Hong
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D002369:Castration; D005260:Female; D015870:Gene Expression; D008297:Male; D020641:Polymorphism, Single Nucleotide; D013251:Steroid Isomerases / Q000235:genetics*; D034421:Sus scrofa / Q000378:metabolism*
chemical_list: D013251:Steroid Isomerases; C022660:steroid delta-isomerase
keywords: 3β-hydroxysteroid dehydrogenase; boar taint; single nucleotide polymorphism