title: Laparoscopic splenic biopsy--porcine to human studies--using a fibrin sealant technique.
abstract: BACKGROUND
  Splenic biopsies are not routinely performed because of the risk of severe hemorrhage. The aim of this study was to explore the feasibility of performing laparoscopic splenic biopsies using a fibrin sealant in pigs and then to translate this technique into the clinical setting.
  
  
  METHODS
  Four German Landrace pigs underwent a laparoscopic splenic biopsy using a fibrin sealant to occlude the needle tract. Time to achieve hemostasis and postoperative hemorrhage were assessed.
  
  
  RESULTS
  The average time to achieve haemostasis was 15 s (range, 8 to 25 s) with no hemorrhage from the needle tract observed. Subsequently this was translated into the clinical setting where a patient also underwent a laparoscopic splenic biopsy without any adverse effect.
  
  
  CONCLUSIONS
  Laparoscopic splenic biopsy with the application of a fibrin sealant is a safe and efficient technique.
authors.affiliation: Department of Hepatopancreatobiliary and Transplantation Surgery, The Freeman Hospital, Newcastle Upon Tyne, Tyne and Wear, UK. kei-yiu.hui@newcastle.ac.uk
authors.forename: Kei Yiu Douglas
authors.initials: KY
authors.lastname: Hui
authors.forename: Stuart M
authors.initials: SM
authors.lastname: Robinson
authors.forename: Aimen
authors.initials: A
authors.lastname: Amer
authors.forename: Jonathan P
authors.initials: JP
authors.lastname: Wallis
authors.forename: Steven A
authors.initials: SA
authors.lastname: White
mesh_terms: D000818:Animals; D001706:Biopsy / Q000379:methods*; D015718:Fibrin Tissue Adhesive / Q000494:pharmacology*; D006489:Hemostatic Techniques; D006801:Humans; D010535:Laparoscopy / Q000379:methods*; D023421:Models, Animal; D011028:Pneumoperitoneum, Artificial / Q000379:methods; D019106:Postoperative Hemorrhage / Q000517:prevention & control*; D018570:Risk Assessment; D013154:Spleen / Q000473:pathology / Q000601:surgery*; D013552:Swine; D013997:Time Factors
chemical_list: D015718:Fibrin Tissue Adhesive