title: Bisphenol A modulates calcium currents and intracellular calcium concentration in rat dorsal root ganglion neurons.
abstract: The endocrine-disrupting chemical bisphenol A (BPA) is used to manufacture plastics including food containers, and it may leach into these containers. Consumption of BPA that has leached out of plastics may be harmful as recent research highlighted that BPA can induce alterations in the nervous system. In the present work, we studied the effects of BPA on Ca²⁺ channels in dorsal root ganglion (DRG) neurons. Using whole-cell patch-clamp recordings, we found that I(Ca) could be reduced by BPA in a concentration-dependent manner. Additionally, BPA shifted the activation curve of calcium currents toward a depolarizing direction and increased the slope factor of the curve. The inactivation curve for the currents was also assessed, and the curve shifted toward the depolarizing direction, although it was not significant. Moreover, inhibitory effects of BPA on the increments of intracellular Ca²⁺ concentrations ([Ca²⁺](i)) induced by 50 mM KCl were observed in DRG neurons using a laser scanning confocal microscopy assay. Further work revealed that the PKA and PKC pathways may be involved in the inhibitory effects of BPA since the PKA antagonist GÖ-6983 and the PKC antagonist H-89 significantly alleviated the inhibitory effects of BPA on I(Ca). As such, the results of the present study provide direct evidence that BPA decreases I(Ca) and impairs calcium homeostasis, which may be involved in any toxic effects of BPA on DRG neurons.
authors.affiliation: School of Public Health, Guiyang Medical University, 9 Beijing Road, Guiyang 550004, China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.forename: Wenhui
authors.initials: W
authors.lastname: Wu
authors.forename: Fei
authors.initials: F
authors.lastname: Huan
authors.forename: Hang
authors.initials: H
authors.lastname: Xiao
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000009:adverse effects / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000009:adverse effects / Q000494:pharmacology*; D005727:Ganglia, Spinal / Q000166:cytology / Q000378:metabolism*; D007211:Indoles / Q000494:pharmacology; D007546:Isoquinolines / Q000494:pharmacology; D008297:Male; D008301:Maleimides / Q000494:pharmacology; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D010636:Phenols / Q000009:adverse effects / Q000494:pharmacology*; D011493:Protein Kinase C / Q000378:metabolism; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013449:Sulfonamides / Q000494:pharmacology; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D002118:Calcium; D017868:Cyclic AMP-Dependent Protein Kinases; D016166:Free Radical Scavengers; D007211:Indoles; D007546:Isoquinolines; D008301:Maleimides; D010636:Phenols; D011493:Protein Kinase C; D047428:Protein Kinase Inhibitors; D013449:Sulfonamides; C465664:2-(1-(3-dimethylaminopropyl)-5-methoxyindol-3-yl)-3-(1H-indol-3-yl)maleimide; C063509:N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide; C006780:bisphenol A; D000098826:Bisphenol A Compounds