title: BLIP-II is a highly potent inhibitor of Klebsiella pneumoniae carbapenemase (KPC-2).
abstract: β-Lactamase inhibitory protein II (BLIP-II) is a potent inhibitor of class A β-lactamases. KPC-2 is a class A β-lactamase that is capable of hydrolyzing carbapenems and has become a widespread source of resistance to these drugs for Gram-negative bacteria. Determination of association and dissociation rate constants for binding between BLIP-II and KPC-2 reveals a very tight interaction with a calculated (koff/kon) equilibrium dissociation constant of 76 fM (76 × 10(-15) M).
authors.affiliation: Department of Biochemistry and Molecular Biology and Department of Pharmacology, Baylor College of Medicine, Houston, Texas, USA.
authors.forename: Nicholas G
authors.initials: NG
authors.lastname: Brown
authors.forename: Dar-Chone
authors.initials: DC
authors.lastname: Chow
authors.forename: Timothy
authors.initials: T
authors.lastname: Palzkill
mesh_terms: D001426:Bacterial Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D015780:Carbapenems / Q000378:metabolism; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000201:enzymology*; D008826:Microbial Sensitivity Tests; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases
chemical_list: D001426:Bacterial Proteins; D015780:Carbapenems; D065093:beta-Lactamase Inhibitors; C065596:beta-lactamase-inhibitor protein, Streptomyces; D001618:beta-Lactamases; C531581:carbapenemase-2, Klebsiella pneumoniae