title: Polymerase synthesis of oligonucleotides containing a single chemically modified nucleobase for site-specific redox labelling.
abstract: Enzymatic construction of single-nucleobase redox-labelled oligonucleotides was developed either based on polymerase incorporation of a single modified nucleoside triphosphate (dNTP) followed by primer extension (PEX) with natural dNTPs or based on PEX with a biotinylated one-nucleotide overhang template, magnetoseparation and the second PEX with a full-length template.
authors.affiliation: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Gilead & IOCB Research Center, Flemingovo nam. 2, CZ-16610 Prague 6, Czech Republic.
authors.forename: Petra
authors.initials: P
authors.lastname: Ménová
authors.forename: Hana
authors.initials: H
authors.lastname: Cahová
authors.forename: Medard
authors.initials: M
authors.lastname: Plucnara
authors.forename: Luděk
authors.initials: L
authors.lastname: Havran
authors.forename: Miroslav
authors.initials: M
authors.lastname: Fojta
authors.forename: Michal
authors.initials: M
authors.lastname: Hocek
mesh_terms: D017931:DNA Primers / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000378:metabolism*; D005296:Ferrous Compounds / Q000737:chemistry; D006160:Guanosine Triphosphate / Q000737:chemistry / Q000378:metabolism; D000075163:Metallocenes; D009578:Nitrobenzenes / Q000737:chemistry; D009711:Nucleotides / Q000737:chemistry*; D009841:Oligonucleotides / Q000096:biosynthesis* / Q000737:chemistry; D010084:Oxidation-Reduction
chemical_list: D017931:DNA Primers; D005296:Ferrous Compounds; D000075163:Metallocenes; D009578:Nitrobenzenes; D009711:Nucleotides; D009841:Oligonucleotides; D006160:Guanosine Triphosphate; C036077:nitrobenzene; D004259:DNA-Directed DNA Polymerase; C004998:ferrocene