title: Oxidative stress and biochemical perturbations induced by insecticides mixture in rat testes.
abstract: The joint action of pyrethroids, lambda-cyhalothrin (LC) in combination with organophosphates, fenitrothione (FNT) on antioxidant defense system and lipid peroxidation biomarkers in rat testes was studied. The results suggest that incubation of testes homogenate with different concentrations of insecticide mixture for different time intervals significantly decreased the activity of antioxidant enzymes, like glutathione S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT), and the level of reduced glutathione (GSH). In addition, a significant inhibition in transaminases (AST, ALT), phosphatases (AcP, AlP) activity and protein content were observed. On the other hand, FNT plus LC increased the cellular lipid peroxidation (LPO) level and the activity of lactate dehydrogenase (LDH). In conclusion, the use of insecticides mixture might cause marked oxidative damage in a concentration and time-dependent manner.
authors.affiliation: Department of Environmental Studies, Institute of Graduate Studies and Research, Alexandria University, Egypt. eldemerdashf@yahoo.com
authors.forename: Fatma M
authors.initials: FM
authors.lastname: El-Demerdash
authors.forename: Ali B
authors.initials: AB
authors.lastname: Jebur
authors.forename: Hoda M
authors.initials: HM
authors.lastname: Nasr
mesh_terms: D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D007306:Insecticides / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009570:Nitriles / Q000494:pharmacology; D010755:Organophosphates / Q000494:pharmacology; D018384:Oxidative Stress / Q000187:drug effects*; D011722:Pyrethrins / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D013737:Testis / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D007306:Insecticides; D009570:Nitriles; D010755:Organophosphates; D011722:Pyrethrins; D007770:L-Lactate Dehydrogenase; D002374:Catalase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D000410:Alanine Transaminase; D005978:Glutathione; C037304:cyhalothrin