title: Comparative mitochondrial proteomic analysis of hepatocellular carcinoma from patients.
abstract: OBJECTIVE
  To define mitochondrial protein markers related to liver cancer.
  
  
  METHODS
  Mitochondrial subproteomes of 20 patient-derived liver carcinoma and tumor-free control tissues were performed by 2DE coupled with MALDI-TOF/TOF. The altered patterns of three identified proteins were validated by Western blot and immunohistochemistry.
  
  
  RESULTS
  The results showed that compared with tumor-free control samples, nine proteins were downregulated and six proteins were upregulated in carcinoma samples. The increased expression of Arg1 mRNA and protein was validated by Western blot, Q-RT-PCR, paraffin tissue microarray and immunohistochemistry. Furthermore, a literature review shows that Heat shock protein 10 (Hsp10), single-stranded DNA-binding protein (SSBP1), and peptidyl-prolyl cis-trans isomerase A (PPIA), which were identified as being increased in the tumor samples in this study, may be closely related to protein folding and translation.
  
  
  CONCLUSIONS
  These results show that in addition to changes in the signaling pathways, such as the Ras-Raf-MEK-ERK pathway, altered mitochondrial DNA replication and protein folding in liver cancer are also worth studying further. Collectively, these results suggest that specific mitochondrial proteins are uniquely susceptible to alterations in expression and carry implications for the investigation of their potential as therapeutic and prognostic markers. Further studies focusing on these proteins will be used to predict treatment response and reverse the apoptosis resistance.
authors.affiliation: Immuno-Oncology Laboratory of Fujian Provincial Cancer Hospital, Fujian Medical University Teaching Hospital, Fujian, P R China. yunbin_ye@yahoo.com
authors.forename: Yunbin
authors.initials: Y
authors.lastname: Ye
authors.forename: Aimin
authors.initials: A
authors.lastname: Huang
authors.forename: Chuanzhong
authors.initials: C
authors.lastname: Huang
authors.forename: Jingfeng
authors.initials: J
authors.lastname: Liu
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Kecan
authors.initials: K
authors.lastname: Lin
authors.forename: Qiang
authors.initials: Q
authors.lastname: Chen
authors.forename: Yongyi
authors.initials: Y
authors.lastname: Zeng
authors.forename: Huijing
authors.initials: H
authors.lastname: Chen
authors.forename: Xuan
authors.initials: X
authors.lastname: Tao
authors.forename: Guangya
authors.initials: G
authors.lastname: Wei
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Wu
mesh_terms: D000328:Adult; D001119:Arginase / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D018835:Chaperonin 10 / Q000235:genetics / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D019295:Computational Biology; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D020935:MAP Kinase Signaling System; D008297:Male; D008875:Middle Aged; D008928:Mitochondria / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D019696:Peptidylprolyl Isomerase / Q000235:genetics / Q000378:metabolism; D017510:Protein Folding; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D018835:Chaperonin 10; D004268:DNA-Binding Proteins; D024101:Mitochondrial Proteins; D001119:Arginase; D019696:Peptidylprolyl Isomerase