title: Feed intake and brain neuropeptide Y (NPY) and cholecystokinin (CCK) gene expression in juvenile cobia fed plant-based protein diets with different lysine to arginine ratios.
abstract: Cobia (Rachycentron canadum, Actinopterygii, Perciformes;10.5±0.1g) were fed to satiation with three plant-based protein test diets with different lysine (L) to arginine (A) ratios (LL/A, 0.8; BL/A, 1.1; and HL/A, 1.8), using a commercial diet as control for six weeks. The test diets contained 730 g kg(-1) plant ingredients with 505-529 g protein, 90.2-93.9 g lipid kg(-1) dry matter; control diet contained 550 g protein and 95 g lipid kg(-1) dry matter. Periprandial expression of brain NPY and CCK (npy and cck) was measured twice (weeks 1 and 6). At week one, npy levels were higher in pre-feeding than postfeeding cobia for all diets, except LL/A. At week six, npy levels in pre-feeding were higher than in postfeeding cobia for all diets. cck in pre-feeding cobia did not differ from that in postfeeding for all diets, at either time point. Cobia fed LL/A had lower feed intake (FI) than cobia fed BL/A and control diet, but no clear correlations between dietary L/A ratio and FI, growth and expression of npy and cck were detected. The data suggest that NPY serves as an orexigenic factor, but further studies are necessary to describe links between dietary L/A and regulation of appetite and FI in cobia.
authors.affiliation: Department of Biology, University of Bergen, Bergen, Norway. Minhntu@gmail.com
authors.forename: Minh Van
authors.initials: MV
authors.lastname: Nguyen
authors.forename: Ann-Elise Olderbakk
authors.initials: AE
authors.lastname: Jordal
authors.forename: Marit
authors.initials: M
authors.lastname: Espe
authors.forename: Louise
authors.initials: L
authors.lastname: Buttle
authors.forename: Hung Van
authors.initials: HV
authors.lastname: Lai
authors.forename: Ivar
authors.initials: I
authors.lastname: Rønnestad
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001120:Arginine / Q000008:administration & dosage* / Q000378:metabolism; D001921:Brain / Q000378:metabolism / Q000502:physiology*; D002766:Cholecystokinin / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D004032:Diet; D004435:Eating / Q000502:physiology; D015870:Gene Expression; D008239:Lysine / Q000008:administration & dosage* / Q000378:metabolism; D008969:Molecular Sequence Data; D009478:Neuropeptide Y / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D010473:Perciformes / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D014674:Plant Proteins, Dietary / Q000008:administration & dosage* / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D009478:Neuropeptide Y; D014674:Plant Proteins, Dietary; D002766:Cholecystokinin; D001120:Arginine; D008239:Lysine