title: Hydrogel of polysaccharide of sugarcane molasses as carrier of bone morphogenetic protein in the reconstruction of critical bone defects in rats.
abstract: OBJECTIVE
  To evaluate the benefit of using carriers such as the biopolymer gel (hidrogel of polysaccharide of sugarcane molasses) associated with the bone morphogenetic proteins (BMP's) in the repair of critical bone defects in calvaria of Wistar rats.
  
  
  METHODS
  Forty-two rats were submitted to a surgical calvaria bone defects. These animals were divided into two experimental groups, positive control group and negative control group. The Group I the calvaria defect was filled up with biopolymer gel, biological membrane, BMP and lyophilized graft. The Group II was treated with biopolymer gel, BMP and lyophilized graft. And the group III (positive control group) was treated with BMP, lyophilized graft and biological membrane. In the negative control group (Group IV) a defect was made in the rat calvaria and the animals were sacrificed immediately after the surgery. The animals of experimental groups and positive control group were slaughtered after subsequent periods of 90 and 180 days. In these periods, the histological analysis and image assessment by cone bean tomographic imaging were obtained.
  
  
  RESULTS
  There was highest bone tissue formation with statistically significant results in the groups that associated biopolymer gel and membrane (Group I), followed by the group III (BMP, lyophilized graft and biological membrane). The lower bone formation occurred in the group not using the sugarcane biopolymer gel (Group II). The radiolucent areas of the analyzes of 180 days among the groups studied were respectively, 14.98 mm(2), 26.65 mm(2) and 35.81 mm(2).
  
  
  CONCLUSIONS
  The biopolymer gel showed to be an excellent bone morphogenetic protein carrier, probably by facilitating the controlled release of these proteins in the process of bone repair.
authors.affiliation: Postgraduate Program in Surgery, UFPE, Recife-PE, Brazil. drmdinoa@yahoo.com.br
authors.forename: Martinho Dinoá
authors.initials: MD
authors.lastname: Medeiros Júnior
authors.forename: Elaine Judite de Amorim
authors.initials: EJ
authors.lastname: Carvalho
authors.forename: Ivson Souza
authors.initials: IS
authors.lastname: Catunda
authors.forename: Sidcley
authors.initials: S
authors.lastname: Bernardino-Araújo
authors.forename: José Lamartine de Andrade
authors.initials: JL
authors.lastname: Aguiar
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage; D001704:Biopolymers / Q000008:administration & dosage; D019485:Bone Morphogenetic Proteins / Q000008:administration & dosage*; D001861:Bone Regeneration / Q000187:drug effects*; D018786:Bone Substitutes / Q000008:administration & dosage; D004337:Drug Carriers / Q000008:administration & dosage*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000008:administration & dosage*; D008297:Male; D011134:Polysaccharides / Q000008:administration & dosage*; D051381:Rats; D015203:Reproducibility of Results; D031786:Saccharum / Q000737:chemistry*; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D001672:Biocompatible Materials; D001704:Biopolymers; D019485:Bone Morphogenetic Proteins; D018786:Bone Substitutes; D004337:Drug Carriers; D011134:Polysaccharides; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate