title: Overexpression of ctr1Δ300, a high-affinity copper transporter with deletion of the cytosolic C-terminus in Saccharomyces cerevisiae under excess copper, leads to disruption of transition metal homeostasis and transcriptional remodelling of cellular processes.
abstract: In an approach to generating Saccharomyces cerevisiae strains with increased intracellular copper amounts for technical applications, we overexpressed the copper transporter CTR1 and a variant of CTR1 with a truncation in the C-terminus after the 300th amino acid (ctr1Δ300). We determined the copper sensitivity of the generated strains and used inductively coupled plasma spectrometry analysis (ICP-OES and ICP-MS) to investigate the effects of overexpression of both constructs under excess copper on the cellular content of different elements in S. cerevisiae. In addition, we performed DNA microarray analysis to obtain the gene expression profile under the changed element contents. Overexpression of CTR1 increased the copper content in the cells to 160% and 78 genes were differentially regulated. Overexpression of the truncated ctr1Δ300 resulted in an increased copper, iron and zinc content of > 200% and 980 genes showed differential expression. We found that transition metal ion homeostasis was disrupted in ctr1Δ300-overexpressing strains under excess copper and that this was combined with a transcriptional remodelling of cellular processes.
authors.affiliation: Institute of Genetics, Dresden University of Technology, Dresden, Germany. Astrid.Schuller@tu-dresden.de
authors.forename: Astrid
authors.initials: A
authors.lastname: Schuller
authors.forename: Gudrun
authors.initials: G
authors.lastname: Auffermann
authors.forename: Katja
authors.initials: K
authors.lastname: Zoschke
authors.forename: Ulrike
authors.initials: U
authors.lastname: Schmidt
authors.forename: Kai
authors.initials: K
authors.lastname: Ostermann
authors.forename: Gerhard
authors.initials: G
authors.lastname: Rödel
mesh_terms: D001692:Biological Transport; D027682:Cation Transport Proteins / Q000235:genetics* / Q000378:metabolism; D003300:Copper / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology; D000079923:Copper Transporter 1; D003600:Cytosol / Q000378:metabolism; D015536:Down-Regulation; D015870:Gene Expression; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects*; D006706:Homeostasis; D007501:Iron / Q000032:analysis / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D011993:Recombinant Fusion Proteins; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D017384:Sequence Deletion; D013054:Spectrophotometry, Atomic; D028561:Transition Elements / Q000032:analysis / Q000378:metabolism; D015854:Up-Regulation; D015032:Zinc / Q000032:analysis / Q000378:metabolism
chemical_list: C490078:CTR1 protein, S cerevisiae; D027682:Cation Transport Proteins; D000079923:Copper Transporter 1; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D028561:Transition Elements; D003300:Copper; D007501:Iron; D015032:Zinc