title: The dynamic pathway of nuclear RNA in eukaryotes.
abstract: The passage of mRNA molecules from the site of synthesis, through the nucleoplasm and the nuclear pore, en route to the cytoplasm, might appear straightforward. Nonetheless, several decades of detailed examination of this pathway, from high resolution electron microscopy in fixed specimens, through the development of immuno-detection techniques and fluorescence toolkits, to the current era of live-cell imaging, show this to be an eventful journey. In addition to mRNAs, several species of noncoding RNAs travel and function in the nucleus, some being retained within throughout their lifetime. This review will highlight the nucleoplasmic paths taken by mRNAs and noncoding RNAs in eukaryotic cells with special focus on live-cell data and in concurrence with the biophysical nature of the nucleus.
authors.affiliation: The Mina & Everard Goodman Faculty of Life Sciences & Institute of Nanotechnology, Bar-Ilan University, Ramat Gan, Israel.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Sheinberger
authors.forename: Yaron
authors.initials: Y
authors.lastname: Shav-Tal
mesh_terms: D021581:Active Transport, Cell Nucleus / Q000235:genetics*; D000818:Animals; D003593:Cytoplasm / Q000378:metabolism* / Q000648:ultrastructure; D005057:Eukaryotic Cells / Q000378:metabolism* / Q000648:ultrastructure; D015870:Gene Expression; D006801:Humans; D022022:Nuclear Pore / Q000378:metabolism* / Q000648:ultrastructure; D029543:Nucleocytoplasmic Transport Proteins / Q000235:genetics / Q000378:metabolism; D010944:Plants / Q000235:genetics; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism*; D015138:RNA, Nuclear / Q000737:chemistry / Q000378:metabolism*; D022661:RNA, Untranslated / Q000737:chemistry / Q000378:metabolism*; D013194:Staining and Labeling; D059008:Time-Lapse Imaging
chemical_list: D029543:Nucleocytoplasmic Transport Proteins; D012333:RNA, Messenger; D015138:RNA, Nuclear; D022661:RNA, Untranslated
keywords: RNA dynamics; inter-chromatin; live-cell imaging; mRNA export; nuceloplasm