title: Role of α-CGRP in the regulation of neurotoxic responses induced by kainic acid in mice.
abstract: Kainic acid (KA) is an excitatory and neurotoxic substance. The role of α-calcitonin gene-related peptide (α-CGRP) in the regulation of KA-induced hippocampal neuronal cell death was investigated in the present study. The intracerebroventricular (i.c.v.) administration with KA (0.07 μg) increased hippocampal α-CGRP mRNA level in ICR mice. The α-CGRP mRNA level began to increase at 1h, reached at maximal level at 6 and 12h, and returned to the control level by 24h after i.c.v. administration with KA. In addition, KA-induced hippocampal CA3 neuronal death in C57BL6 (wild type) group was more pronounced compared to KA-induced hippocampal CA3 pyramidal cell death in α-CGRP knock-out (KO) group. Furthermore, sumatriptan, a CGRP releasing inhibitor, significantly protected the pyramidal cell death in CA3 hippocampal region induced by KA administered i.c.v. in ICR mice. Our results suggest that α-CGRP may play an important role in the regulation of KA-induced pyramidal cell death in CA3 region of the hippocampus.
authors.affiliation: Institute of Natural Medicine, College of Medicine, Hallym University, Chuncheon 200-702, Republic of Korea.
authors.forename: Soo-Hyun
authors.initials: SH
authors.lastname: Park
authors.forename: Yun-Beom
authors.initials: YB
authors.lastname: Sim
authors.forename: Chea-Ha
authors.initials: CH
authors.lastname: Kim
authors.forename: Jin-Koo
authors.initials: JK
authors.lastname: Lee
authors.forename: Jong-Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: Hong-Won
authors.initials: HW
authors.lastname: Suh
mesh_terms: D000818:Animals; D056654:CA3 Region, Hippocampal / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D015740:Calcitonin Gene-Related Peptide / Q000502:physiology*; D016923:Cell Death / Q000187:drug effects; D018690:Excitatory Amino Acid Agonists / Q000633:toxicity*; D015870:Gene Expression / Q000187:drug effects; D007608:Kainic Acid / Q000633:toxicity*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D018345:Mice, Knockout; D017966:Pyramidal Cells / Q000187:drug effects / Q000502:physiology; D018170:Sumatriptan / Q000494:pharmacology
chemical_list: D018690:Excitatory Amino Acid Agonists; D018170:Sumatriptan; D015740:Calcitonin Gene-Related Peptide; D007608:Kainic Acid