title: Isolation of DNA, RNA and protein from the starlet sea anemone Nematostella vectensis.
abstract: Among marine invertebrates, the starlet sea anemone Nematostella vectensis has emerged as an important laboratory model system. One advantage of working with this species relative to many other marine invertebrates is the ease of isolating relatively pure DNA, RNA and protein. Nematostella can be raised at high densities, under clean culture conditions, and it lacks integumentary or skeletal structures that can impede the recovery of DNA, RNA or protein. Here we describe methods used in our lab to isolate DNA, RNA and protein from Nematostella embryos, larvae and adults. The methods described here are less expensive than commercial kits and are more easily scalable to larger tissue amounts. Preparation of DNA can be completed in ∼7 h, RNA preparation in ∼1.5 h and protein preparation in ∼1 h.
authors.affiliation: Department of Biology, Boston University, Boston, Massachusetts, USA.
authors.forename: Derek J
authors.initials: DJ
authors.lastname: Stefanik
authors.forename: Francis S
authors.initials: FS
authors.lastname: Wolenski
authors.forename: Lauren E
authors.initials: LE
authors.lastname: Friedman
authors.forename: Thomas D
authors.initials: TD
authors.lastname: Gilmore
authors.forename: John R
authors.initials: JR
authors.lastname: Finnerty
mesh_terms: D000818:Animals; D004247:DNA / Q000302:isolation & purification*; D005821:Genetic Techniques; D007814:Larva / Q000235:genetics; D011506:Proteins / Q000302:isolation & purification*; D012313:RNA / Q000302:isolation & purification*; D012615:Sea Anemones / Q000196:embryology / Q000235:genetics*
chemical_list: D011506:Proteins; D012313:RNA; D004247:DNA