title: Neuroendocrine regulation and metabolism of glucose and lipids in primary chronic insomnia: a prospective case-control study.
abstract: OBJECTIVE
  To investigate the relation between primary chronic insomnia and insulin sensitivity, visceral adiposity, non alcoholic fatty liver disease and neuroendocrine hormones.
  
  
  METHODS
  In a case-controlled, prospective clinical trial 13 women with primary chronic insomnia according to DSM-IV criteria were compared to 12 healthy controls matched for age, sex, BMI, body composition and menopausal status. All participants had a sleep assessment including polysomnographic studies and neuropsychiatric evaluation. Insulin sensitivity was evaluated using the euglycaemic hyperinsulinemic clamp. Hepatic fat content, visceral adipose tissue and intramyocellular lipid accumulation were assessed using magnetic resonance imaging and spectroscopy. The hormonal stress axis was evaluated by measurements of midnight and early morning salivary cortisol, urinary catecholamines and plasma metanephrines. Body composition was determined using body impedance analysis and indirect calorimetry.
  
  
  RESULTS
  Although the diagnosis of primary chronic insomnia was made by established clinical criteria, standard polysomongraphic studies failed to identify altered sleep continuity and architecture when compared to matched controls. However, women with primary chronic insomnia showed significantly higher midnight salivary cortisol concentrations (1.46 vs. 0.76 nmol/l, p = 0.02), indicating dysregulation of the hypothalamo-pituitary-adrenal (HPA) axis. Plasma glucose and lipid concentrations, insulin sensitivity, hepatic and intramyocellular fat content, visceral adipose tissue mass and body composition did not differ between the two groups.
  
  
  CONCLUSIONS
  Healthy women with clinically diagnosed primary chronic insomnia demonstrate a dysregulation of circadian cortisol secretion despite normal sleep continuity and architecture. Increased midnight cortisol levels, however, were not associated with impaired metabolism of glucose and lipids.
authors.affiliation: Endocrinology, Diabetology and Metabolism, University Hospital Basel, Basel, Switzerland. seelige@uhbs.ch
authors.forename: Eleonora
authors.initials: E
authors.lastname: Seelig
authors.forename: Ulrich
authors.initials: U
authors.lastname: Keller
authors.forename: Markus
authors.initials: M
authors.lastname: Klarhöfer
authors.forename: Klaus
authors.initials: K
authors.lastname: Scheffler
authors.forename: Serge
authors.initials: S
authors.lastname: Brand
authors.forename: Edith
authors.initials: E
authors.lastname: Holsboer-Trachsler
authors.forename: Martin
authors.initials: M
authors.lastname: Hatzinger
authors.forename: Stefan
authors.initials: S
authors.lastname: Bilz
mesh_terms: D001823:Body Composition; D016022:Case-Control Studies; D002395:Catecholamines / Q000652:urine; D017097:Electric Impedance; D004734:Energy Metabolism; D005260:Female; D005947:Glucose / Q000378:metabolism*; D015309:Glucose Clamp Technique; D006801:Humans; D006854:Hydrocortisone / Q000378:metabolism; D006946:Hyperinsulinism / Q000150:complications; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008676:Metanephrine / Q000097:blood; D008875:Middle Aged; D009132:Muscles / Q000378:metabolism; D009490:Neurosecretory Systems / Q000378:metabolism*; D017286:Polysomnography; D011446:Prospective Studies; D012146:Rest; D012890:Sleep; D007319:Sleep Initiation and Maintenance Disorders / Q000097:blood / Q000378:metabolism* / Q000503:physiopathology / Q000652:urine
chemical_list: D002395:Catecholamines; D008676:Metanephrine; D005947:Glucose; D006854:Hydrocortisone