title: Protective effects of osthole, a natural derivative of coumarin, against intestinal ischemia-reperfusion injury in mice.
abstract: Intestinal ischemia/reperfusion (I/R) injury is considered to be associated with high morbidity and mortality rates. Osthole, a natural derivative of coumarin, has been shown to exert a variety of pharmacological and therapeutic effects under physiological and pathological conditions. In the present study, to investigate the protective effects of osthole against intestinal I/R injury, various doses of osthole (5, 10, 25 and 50 mg/kg) were pre-administered to mice subjected to intestinal I/R injury. A dose-dependent increase in the survival rate was observed in the osthole-treated mice. Pre-treatment with osthole (50 mg/kg) attenuated the destruction of epithelial cells within the villi induced by intestinal I/R injury, and suppressed oxidative stress, neutrophil infiltration and modulated nitric oxide (NO) levels. Moreover, the increased IκBα phosphorylation and nuclear factor (NF)-κB nuclear translocation induced by I/R injury were significantly decreased following pre-treatment with osthole. Taken together, our data demonstrate that osthole exerts protective effects against intestinal I/R injury in mice by suppressing oxidative stress, neutrophil infiltration and NO levels, partly through the inhibition of NF-κB nuclear translocation. Hence, the findings of the present study provide insight into the mechanisms through which osthole exerts its protective effects against intestinal I/R injury.
authors.affiliation: Department of Cardiovascular Surgery, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, People's Republic of China.
authors.forename: Wenpeng
authors.initials: W
authors.lastname: Dong
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.forename: Zhengjun
authors.initials: Z
authors.lastname: Liu
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.forename: Xianyue
authors.initials: X
authors.lastname: Wang
authors.forename: Shenghui
authors.initials: S
authors.lastname: Bi
authors.forename: Xiaowu
authors.initials: X
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Ma
authors.forename: Weida
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology; D003374:Coumarins / Q000008:administration & dosage / Q000494:pharmacology*; D004195:Disease Models, Animal; D007422:Intestines / Q000098:blood supply* / Q000187:drug effects / Q000473:pathology*; D008297:Male; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D020556:Neutrophil Infiltration / Q000187:drug effects; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D015427:Reperfusion Injury / Q000378:metabolism / Q000401:mortality / Q000517:prevention & control*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000975:Antioxidants; D003374:Coumarins; D016328:NF-kappa B; D009569:Nitric Oxide; C046627:osthol