title: JC polyomavirus infection in candidates for kidney transplantation living in the Brazilian Amazon region.
abstract: This study evaluated the relative occurrences of BK virus (BKV) and JC virus (JCV) infections in patients with chronic kidney disease (CKD). Urine samples were analysed from CKD patients and from 99 patients without CKD as a control. A total of 100 urine samples were analysed from the experimental (CKD patients) group and 99 from the control group. Following DNA extraction, polymerase chain reaction (PCR) was used to amplify a 173 bp region of the gene encoding the T antigen of the BKV and JCV. JCV and BKV infections were differentiated based on the enzymatic digestion of the amplified products using BamHI endonuclease. The results indicated that none of the patients in either group was infected with the BKV, whereas 11.1% (11/99) of the control group subjects and 4% (4/100) of the kidney patients were infected with the JCV. High levels of urea in the excreted urine, low urinary cellularity, reduced bladder washout and a delay in analysing the samples may have contributed to the low prevalence of infection. The results indicate that there is a need to increase the sensitivity of assays used to detect viruses in patients with CDK, especially given that polyomavirus infections, especially BKV, can lead to a loss of kidney function following transplantation.
authors.affiliation: Hospital das Clínicas do Acre, Rio Branco, AC, Brasil.
authors.forename: Fernando Assis Ferreira
authors.initials: FA
authors.lastname: Melo
authors.forename: Ana Caroline Fonseca
authors.initials: AC
authors.lastname: Bezerra
authors.forename: Bárbara Brasil
authors.initials: BB
authors.lastname: Santana
authors.forename: Marluísa Oliveira Guimarães
authors.initials: MO
authors.lastname: Ishak
authors.forename: Ricardo
authors.initials: R
authors.lastname: Ishak
authors.forename: Izaura Maria Vieira
authors.initials: IM
authors.lastname: Cayres-Vallinoto
authors.forename: Antonio Carlos Rosário
authors.initials: AC
authors.lastname: Vallinoto
mesh_terms: D000328:Adult; D000368:Aged; D001739:BK Virus / Q000302:isolation & purification*; D016022:Case-Control Studies; D004279:DNA, Viral / Q000032:analysis; D005260:Female; D006801:Humans; D007577:JC Virus / Q000302:isolation & purification*; D007676:Kidney Failure, Chronic / Q000150:complications* / Q000652:urine; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D027601:Polyomavirus Infections / Q000150:complications / Q000175:diagnosis*; D014412:Tumor Virus Infections / Q000150:complications / Q000175:diagnosis*; D055815:Young Adult
chemical_list: D004279:DNA, Viral