title: Comparative analysis of in vitro periodontal characteristics of stem cells from apical papilla (SCAP) and periodontal ligament stem cells (PDLSCs).
abstract: OBJECTIVE
  The aim of this study was to compare the in vitro periodontal properties of stem cells from apical papilla (SCAP) and periodontal ligament stem cells (PDLSCs).
  
  
  METHODS
  SCAP and PDLSCs cultures were established from normal human impacted third molars with immature roots. The cells were cultured in differentiation medium containing dexamethasone, ß-glycerophosphate and ascorbate phosphate for 3 weeks and in normal medium for as long as 60 days, and then were analysed for mineralisation potential. Cell proliferation, colony-forming capacity and periodontal ligament (PDL)-specific markers were also measured. The mineralisation markers, including alkaline phosphatase (ALP), bone sialoprotein (BSP) and osteocalcin (OC), were investigated by immunofluorescence staining and real-time polymerase chain reaction. The expression of PDL markers, including periostin and S100A4, was confirmed by reverse transcription polymerase chain reaction.
  
  
  RESULTS
  SCAP showed a significantly higher proliferation rate and colony-forming capacity than PDLSCs. Both types of cells displayed mineralisation potential after induction and long-term culture. The SCAP, however, exhibited higher levels of ALP, BSP and OC expression than the PDLSCs. Like the PDLSCs, the SCAP exhibited periostin and S100A4 expression.
  
  
  CONCLUSIONS
  Our study provides the first evidence showing that SCAP express periodontal properties in vitro. SCAP not only showed PDL-related markers, but also displayed a higher proliferation rate and a greater mineralisation capacity than those of PDLSCs. It might help understand the development of tooth root and periodontium. Furthermore, SCAP could be a promising candidate for periodontal tissue engineering.
authors.affiliation: Department of Stomatology, Guangzhou Women and Children's Medical Center, 9 Jinsui Road, Guangzhou 510623, PR China. dentchenke@sohu.com
authors.forename: Ke
authors.initials: K
authors.lastname: Chen
authors.forename: Huacui
authors.initials: H
authors.lastname: Xiong
authors.forename: Yibin
authors.initials: Y
authors.lastname: Huang
authors.forename: Caiqi
authors.initials: C
authors.lastname: Liu
mesh_terms: D000293:Adolescent; D000469:Alkaline Phosphatase / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D002113:Calcification, Physiologic / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000032:analysis; D002449:Cell Aggregation / Q000502:physiology; D002452:Cell Count; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D003470:Culture Media; D003771:Dental Papilla / Q000166:cytology*; D006801:Humans; D058574:Integrin-Binding Sialoprotein / Q000032:analysis; D015675:Osteocalcin / Q000032:analysis; D010513:Periodontal Ligament / Q000166:cytology*; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins / Q000032:analysis; D013234:Stem Cells / Q000502:physiology*; D013997:Time Factors; D055815:Young Adult
chemical_list: D015415:Biomarkers; D015815:Cell Adhesion Molecules; D003470:Culture Media; D058574:Integrin-Binding Sialoprotein; C512835:POSTN protein, human; D000071999:S100 Calcium-Binding Protein A4; D009418:S100 Proteins; D015675:Osteocalcin; C077591:S100A4 protein, human; D000469:Alkaline Phosphatase