title: The role of hnRPUL1 involved in DNA damage response is related to PARP1.
abstract: Heterogeneous nuclear ribonucleoprotein U-like 1 (hnRPUL1) -also known as adenovirus early region 1B-associated proteins 5 (E1B-AP5) - plays a role in RNA metabolism. Recently, hnRPUL1 has also been shown to be involved in DNA damage response, but the function of hnRPUL1 in response to DNA damage remains unclear. Here, we have demonstrated that hnRPUL1 is associated with PARP1 and recruited to DNA double-strand breaks (DSBs) sites in a PARP1-mediated poly (ADP-ribosyl) ation dependent manner. In turn, hnRPUL1 knockdown enhances the recruitment of PARP1 to DSBs sites. Specifically, we showed that hnRPUL1 is also implicated in the transcriptional regulation of PARP1 gene. Thus, we propose hnRPUL1 as a new component related to PARP1 in DNA damage response and repair.
authors.affiliation: Department of Genetics and Developmental Biology, Medical School of Southeast University, The Key Laboratory of Developmental Genes and Human Disease in Ministry of Education, Nanjing, China. hongzehui@seu.edu.cn
authors.forename: Zehui
authors.initials: Z
authors.lastname: Hong
authors.forename: Jie
authors.initials: J
authors.lastname: Jiang
authors.forename: Jiaolong
authors.initials: J
authors.lastname: Ma
authors.forename: Shikui
authors.initials: S
authors.lastname: Dai
authors.forename: Ting
authors.initials: T
authors.lastname: Xu
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Akira
authors.initials: A
authors.lastname: Yasui
mesh_terms: D000244:Adenosine Diphosphate / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D053903:DNA Breaks, Double-Stranded; D004249:DNA Damage; D015536:Down-Regulation / Q000235:genetics; D042822:Genomic Instability; D034441:Heterogeneous-Nuclear Ribonucleoproteins / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D051379:Mice; D009687:Nuclear Proteins / Q000737:chemistry / Q000378:metabolism*; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport / Q000235:genetics; D012266:Ribose / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: C475078:HNRNPUL1 protein, human; D034441:Heterogeneous-Nuclear Ribonucleoproteins; D009687:Nuclear Proteins; D014157:Transcription Factors; D000244:Adenosine Diphosphate; D012266:Ribose; C499320:PARP1 protein, human; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases