title: Assessment of simulated high-dose partial-body irradiation by PCC-R assay.
abstract: The estimation of the dose and the irradiated fraction of the body is important information in the primary medical response in case of a radiological accident. The PCC-R assay has been developed for high-dose estimations, but little attention has been given to its applicability for partial-body irradiations. In the present work we estimated the doses and the percentage of the irradiated fraction in simulated partial-body radiation exposures at high doses using the PCC-R assay. Peripheral whole blood of three healthy donors was exposed to doses from 0-20 Gy, with ⁶⁰Co gamma radiation. To simulate partial body irradiations, irradiated and non-irradiated blood was mixed to obtain proportions of irradiated blood from 10-90%. Lymphocyte cultures were treated with Colcemid and Calyculin-A before harvest. Conventional and triage scores were performed for each dose, proportion of irradiated blood and donor. The Papworth's u test was used to evaluate the PCC-R distribution per cell. A dose-response relationship was fitted according to the maximum likelihood method using the frequencies of PCC-R obtained from 100% irradiated blood. The dose to the partially irradiated blood was estimated using the Contaminated Poisson method. A new D₀ value of 10.9 Gy was calculated and used to estimate the initial fraction of irradiated cells. The results presented here indicate that by PCC-R it is possible to distinguish between simulated partial- and whole-body irradiations by the u-test, and to accurately estimate the dose from 10-20 Gy, and the initial fraction of irradiated cells in the interval from 10-90%.
authors.affiliation: Centro de Protección e Higiene de las Radiaciones, Calle 20 No. 4113, e/41 y 47, Playa, CP 11300, La Habana, Cuba.
authors.forename: Ivonne
authors.initials: I
authors.lastname: Romero
authors.forename: Omar
authors.initials: O
authors.lastname: García
authors.forename: Ana I
authors.initials: AI
authors.lastname: Lamadrid
authors.forename: Eric
authors.initials: E
authors.lastname: Gregoire
authors.forename: Jorge E
authors.initials: JE
authors.lastname: González
authors.forename: Wilfredo
authors.initials: W
authors.lastname: Morales
authors.forename: Cécile
authors.initials: C
authors.lastname: Martin
authors.forename: Joan-Francesc
authors.initials: JF
authors.lastname: Barquinero
authors.forename: Philippe
authors.initials: P
authors.lastname: Voisin
mesh_terms: D001681:Biological Assay / Q000379:methods*; D001822:Body Burden; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000528:radiation effects*; D020732:Cytogenetic Analysis / Q000379:methods*; D003627:Data Interpretation, Statistical; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000502:physiology* / Q000528:radiation effects*; D011829:Radiation Dosage; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014915:Whole-Body Counting / Q000379:methods*; D014916:Whole-Body Irradiation / Q000379:methods
keywords: Calyculin A; D0 value; PCC-R; high doses radiation; partial irradiation