title: [Analysis of the molecular characteristics and cloning of full-length coding sequence of interleukin-2 in tree shrews].
abstract: While the tree shrew (Tupaia belangeri chinensis) is an excellent animal model for studying the mechanisms of human diseases, but few studies examine interleukin-2 (IL-2), an important immune factor in disease model evaluation. In this study, a 465 bp of the full-length IL-2 cDNA encoding sequence was cloned from the RNA of tree shrew spleen lymphocytes, which were then cultivated and stimulated with ConA (concanavalin). Clustal W 2.0 was used to compare and analyze the sequence and molecular characteristics, and establish the similarity of the overall structure of IL-2 between tree shrews and other mammals. The homology of the IL-2 nucleotide sequence between tree shrews and humans was 93%, and the amino acid homology was 80%. The phylogenetic tree results, derived through the Neighbour-Joining method using MEGA5.0, indicated a close genetic relationship between tree shrews, Homo sapiens, and Macaca mulatta. The three-dimensional structure analysis showed that the surface charges in most regions of tree shrew IL-2 were similar to between tree shrews and humans; however, the N-glycosylation sites and local structures were different, which may affect antibody binding. These results provide a fundamental basis for the future study of IL-2 monoclonal antibody in tree shrews, thereby improving their utility as a model.
authors.affiliation: Institute of Medical Biology, the Chinese Academy of Medical Science, China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Huang
authors.forename: Ming-Li
authors.initials: ML
authors.lastname: Li
authors.forename: Juan
authors.initials: J
authors.lastname: Xu
authors.forename: Yue-Dong
authors.initials: YD
authors.lastname: Gao
authors.forename: Wen-Guang
authors.initials: WG
authors.lastname: Wang
authors.forename: An-Guo
authors.initials: AG
authors.lastname: Yin
authors.forename: Xiao-Fei
authors.initials: XF
authors.lastname: Li
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Sun
authors.forename: Xue-Shan
authors.initials: XS
authors.lastname: Xia
authors.forename: Jie-Jie
authors.initials: JJ
authors.lastname: Dai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D003001:Cloning, Molecular; D006801:Humans; D007376:Interleukin-2 / Q000737:chemistry / Q000235:genetics*; D008322:Mammals / Q000145:classification / Q000235:genetics; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D051381:Rats; D016415:Sequence Alignment; D014418:Tupaiidae / Q000145:classification / Q000235:genetics*
chemical_list: D007376:Interleukin-2
keywords: Cloning; Function; Interleukin 2; Structure; Tree shrews