title: Enhanced lipid productivity of Chlorella pyrenoidosa through the culture strategy of semi-continuous cultivation with nitrogen limitation and pH control by CO2.
abstract: Microalgae cultivation with high lipid productivity has received much attention in recent years owing to the economic potential of biofuels production and CO2 emission reduction. Previous studies had reported that the ways of pH-regulation, nutrition-limitation and semi-continuous culture mode can either increase the cells growth rate or promote lipid accumulation. In this study, the novel culture strategy of integrating both nutrition limitation and pH-regulation by CO2 in a semi-continuous cultivation was investigated for enhancing the lipid productivity. In the batch culture, the best growth performance was achieved by controlling pH at 7; lipid contents of the cells can be increased under the nitrogen-limitation conditions. The maximum lipid productivity of 115 mg L(-1) d(-1) was achieved in the novel culture strategy of semi-continuous cultivation with nitrogen-limitation and pH-regulation by CO2, which was 3.64-fold higher than that in the batch culture without pH control and nitrogen limitation.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China. sophyfeifei@126.com
authors.forename: Feifei
authors.initials: F
authors.lastname: Han
authors.forename: Jianke
authors.initials: J
authors.lastname: Huang
authors.forename: Yuanguang
authors.initials: Y
authors.lastname: Li
authors.forename: Weiliang
authors.initials: W
authors.lastname: Wang
authors.forename: Minxi
authors.initials: M
authors.lastname: Wan
authors.forename: Guomin
authors.initials: G
authors.lastname: Shen
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
mesh_terms: D061249:Batch Cell Culture Techniques; D018533:Biomass; D002245:Carbon Dioxide / Q000494:pharmacology*; D018929:Cell Culture Techniques / Q000379:methods*; D002708:Chlorella / Q000187:drug effects / Q000254:growth & development*; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D008055:Lipids / Q000096:biosynthesis*; D009584:Nitrogen / Q000494:pharmacology*; D058433:Photobioreactors / Q000382:microbiology; D013997:Time Factors
chemical_list: D008055:Lipids; D002245:Carbon Dioxide; D009584:Nitrogen