title: MicroRNA-24 inhibits osteosarcoma cell proliferation both in vitro and in vivo by targeting LPAATβ.
abstract: Lysophosphatidic Acid Acyltransferase β (LPAATβ) may be critically involved in osteosarcoma cell proliferation. However, the comprehensive mechanisms responsible for regulation of LPAATβ in osteosarcoma cells remain unclear. This study found that enhanced LPAATβ expression was correlated with osteosarcoma cell proliferation. MiR-24, targeted to LPAATβ, was down-regulated in osteosarcoma cells. Overexpression of miR-24 down-regulated LPAATβ expression in osteosarcoma cells. Specifically, overexpression of miR-24 inhibited osteosarcoma cell proliferation, however, such effect was blocked when LPAATβ activity was inhibited. In conclusion, our study indicates that miR-24 is reduced in osteosarcoma cells, contributing to up-regulation of LPAATβ and resultant osteosarcoma cell proliferation.
authors.affiliation: Department of Orthopaedics, First Affiliated Hospital, Third Military Medical University, Chongqing, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Song
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Ping
authors.initials: P
authors.lastname: Duan
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Xu
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Luo
authors.forename: Fei
authors.initials: F
authors.lastname: Luo
authors.forename: Zehua
authors.initials: Z
authors.lastname: Zhang
authors.forename: Tianyong
authors.initials: T
authors.lastname: Hou
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhou
mesh_terms: D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001859:Bone Neoplasms / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000378:metabolism*; D009368:Neoplasm Transplantation; D012516:Osteosarcoma / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D014183:Transplantation, Heterologous; D015854:Up-Regulation
chemical_list: C543845:MIRN24 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D000217:Acyltransferases; C018764:2-acylglycerophosphate acyltransferase