title: Expanding the glycoengineering toolbox: the rise of bacterial N-linked protein glycosylation.
abstract: Glycosylation is the most prevalent post-translational modification found on proteins, occurring in all domains of life. Ever since the discovery of asparagine-linked (N-linked) protein glycosylation pathways in bacteria, major efforts have been made to harness these systems for the creation of novel therapeutics, vaccines, and diagnostics. Recent advances such as the ability to produce designer glycans in bacteria, some containing unnatural sugars, and techniques for evolving glycosylation enzymes have spawned an entirely new discipline known as bacterial glycoengineering. In addition to their biotechnological and therapeutic potential, bacteria equipped with recombinant N-linked glycosylation pathways are improving our understanding of the N-glycosylation process. This review discusses the key role played by microorganisms in glycosciences, particularly in the context of N-linked glycosylation.
authors.affiliation: School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY 14853, USA.
authors.forename: Jenny L
authors.initials: JL
authors.lastname: Baker
authors.forename: Eda
authors.initials: E
authors.lastname: Çelik
authors.forename: Matthew P
authors.initials: MP
authors.lastname: DeLisa
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D001709:Biotechnology; D006023:Glycoproteins / Q000378:metabolism*; D006031:Glycosylation; D060847:Metabolic Engineering
chemical_list: D001426:Bacterial Proteins; D006023:Glycoproteins