title: Effect of concentration and molecular weight of chitosan and its derivative on the free radical scavenging ability.
abstract: Chitosan is a biodegradable and biocompatible natural scaffold material, which has numerous applications in biomedical sciences. In this study, the in vitro antioxidant activity of chitosan scaffold material was investigated by the chemiluminescence signal generated from the hydroxyl radical (•OH) scavenging assay. The scavenging mechanism was also discussed. The results indicated that the free radical scavenging ability of chitosan scaffold material significantly depends on the chitosan concentration and shows interesting kinetic change. Within the experimental concentration range, the optimal concentration of chitosan was 0.2 mg/mL. The molecular weight of chitosan also attributed to the free radical scavenging ability. Comparison between chitosan and its derivative found that carboxymethyl chitosan possessed higher scavenging ability.
authors.affiliation: School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, China.
authors.forename: Huili
authors.initials: H
authors.lastname: Li
authors.forename: Qing
authors.initials: Q
authors.lastname: Xu
authors.forename: Yun
authors.initials: Y
authors.lastname: Chen
authors.forename: Ajun
authors.initials: A
authors.lastname: Wan
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology*; D048271:Chitosan / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000494:pharmacology*; D017665:Hydroxyl Radical / Q000737:chemistry; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D016166:Free Radical Scavengers; C514968:carboxymethyl-chitosan; D017665:Hydroxyl Radical; D048271:Chitosan
keywords: carboxymethyl chitosan; chitosan; hydroxyl radical scavenging; scaffold