title: 3D arrays for high throughput assay of cell migration and electrotaxis.
abstract: Cell behaviour in 3D environments can be significantly different from those in 2D cultures. With many different 3D matrices being developed and many experimental modalities used to modulate cell behaviour in 3D, it is necessary to develop high throughput techniques to study behaviour in 3D. We report on a 3D array on slide and have adapted this to our electrotaxis chamber, thereby offering a novel approach to quantify cellular responses to electric fields (EFs) in 3D conditions, in different matrices, with different strains of cells, under various field strengths. These developments used Dictyostelium cells to illustrate possible applications and limitations.
authors.affiliation: Laboratory of Regenerative Biology, Key Laboratory of Yunnan for Biomass Energy and Biotechnology of Environment, School of Life Sciences, Yunnan Normal University, Kunming 650500, China.
authors.forename: Sanjun
authors.initials: S
authors.lastname: Zhao
authors.forename: Runchi
authors.initials: R
authors.lastname: Gao
authors.forename: Peter N
authors.initials: PN
authors.lastname: Devreotes
authors.forename: Alex
authors.initials: A
authors.lastname: Mogilner
authors.forename: Min
authors.initials: M
authors.lastname: Zhao
mesh_terms: D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D002465:Cell Movement; D004023:Dictyostelium / Q000166:cytology* / Q000502:physiology; D004560:Electricity; D004574:Electromagnetic Fields; D057166:High-Throughput Screening Assays; D056656:Lab-On-A-Chip Devices; D012685:Sepharose / Q000737:chemistry; D059008:Time-Lapse Imaging
chemical_list: D012685:Sepharose
keywords: 3D matrices; array; assay; cell locomotion; electrotaxis; morphology