title: [Determination of dehydroabietic acid and abietic acid in aqueous alkali extract of Liquidambaris Resina by HPLC].
abstract: OBJECTIVE
  To develop an HPLC method for content determination of dehydroabietic acid and abietic acid in aqueous alkali extract of Liquidambaris Resina.
  
  
  METHODS
  The determination was carried out on a DIONEX C18 column (4.6 mm x 250 mm, 5 microm) eluted with acetonitrile and water containing 0.1% acetic acid. The flow rate was 1 mL x min(-1), and the detected wavelength was set at 210, 240 nm.
  
  
  RESULTS
  The peak areas and the sample quantity of the two components had good linear relationship in the range of 0.4-3.4 microg for dehydroabietic acid, and 0.6-4.8 microg for abietic acid. The average recoveries were 99.53%, 101.9%, respectively.
  
  
  CONCLUSIONS
  The method was proved to be simple, accurate and used for the quality evaluation of Liquidambaris Resina.
authors.affiliation: Capital Normal University, Beijing 100048, China. feng-yw@263.net
authors.forename: Ying-Feng
authors.initials: YF
authors.lastname: Wang
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Wei
mesh_terms: D045784:Abietanes / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D031317:Liquidambar / Q000737:chemistry*
chemical_list: D045784:Abietanes; D004365:Drugs, Chinese Herbal; C013913:dehydroabietic acid; C023710:abietic acid