title: Tumor-associated lymphatic endothelial cell promotes invasion of cervical cancer cells.
abstract: The most common way for cervical cancer to spread is through the lymphatic system. Tumor-associated lymphatic endothelial cell (TLEC) has been considered to play a crucial role in metastasis of certain cancers. The aim of this study was to isolate TLEC from human cervical cancers and explore its involvement in metastasis-associated behaviors in vitro. Lymphatic vessels in 62 cervix tissue specimens ranging from cervical intraepithelial neoplasia (CIN) to advanced invasive cancer were detected using immunochemical staining with D2-40 antibody. Relation of lymphatic vessel density (LVD) to clinicopathological characters was analyzed. Primary TLECs were isolated by LYVE-1 immuno-magnetic beads from cervical cancer tissues and verified through expression of LEC markers Prox-1 and D2-40, and then cultured in vitro. Invasiveness and viability of cells were assessed by transwell assay and typan blue exclusion, respectively. Our results showed that higher LVD was significantly associated with advanced FIGO stage, pelvic lymphatic nodal metastasis (LNM), and poorer cell differentiation. TLECs were successfully primarily isolated and cultured in vitro. Supernatant of TLEC enhanced invasiveness of Hela cell, but did not significantly affect cell viability. In conclusion, TLECs might actively promote lymphatic metastasis of cervical cancer. Further studies are needed to demonstrate the underlying mechanisms.
authors.affiliation: Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
authors.forename: Liqiong
authors.initials: L
authors.lastname: Cai
authors.forename: Shouhua
authors.initials: S
authors.lastname: Yang
authors.forename: Hui
authors.initials: H
authors.lastname: Ding
authors.forename: Jing
authors.initials: J
authors.lastname: Cai
authors.forename: Zehua
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D058846:Antibodies, Monoclonal, Murine-Derived / Q000276:immunology; D014408:Biomarkers, Tumor / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival; D002584:Cervix Uteri / Q000473:pathology*; D042783:Endothelial Cells / Q000473:pathology*; D005260:Female; D006367:HeLa Cells; D018398:Homeodomain Proteins / Q000096:biosynthesis; D006801:Humans; D008207:Lymphatic Metastasis; D042601:Lymphatic Vessels / Q000473:pathology*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis; D002583:Uterine Cervical Neoplasms / Q000473:pathology*; D055815:Young Adult; D002578:Uterine Cervical Dysplasia / Q000473:pathology*; D000098551:Prospero-Related Homeobox 1 Protein
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D014408:Biomarkers, Tumor; D018398:Homeodomain Proteins; D025521:Tumor Suppressor Proteins; C500987:monoclonal antibody D2-40; D000098551:Prospero-Related Homeobox 1 Protein
keywords: Cervical cancer; cell invasiveness; cell viability; lymphatic endothelial cell; lymphatic nodal metastasis; lymphatic vessel density