title: Humoral immune responses to CTL epitope peptides from tumor-associated antigens are widely detectable in humans: a new biomarker for overall survival of patients with malignant diseases.
abstract: Both cellular and humoral immune responses are crucial to induce potent anti-tumor immunity, but most of currently conducted peptide-based cancer vaccines paid attention to cellular responses alone, and none of them are yet approved as a therapeutic modality against cancer patients. We investigated humoral immune responses to CTL epitope peptides derived from tumor-associated antigens in healthy donors and patients with various diseases to facilitate better understanding of their distribution patterns and potential roles. Bead-based multiplex assay, ELISA, and Western blotting were used to measure immunoglobulins reactive to each of 31 different CTL epitope peptides. Importantly, the sums of anti-peptide IgG levels specific to 31 CTL epitope peptides were well correlated with better overall survival (OS) in patients with malignant diseases. Our results suggested that humoral immune responses to CTL epitope peptides were widely detectable in humans. Measurement of immunoglobulins specific to CTL epitope peptides may provide a new biomarker for OS of patients with malignant diseases, although it still remains to be determined whether the correlations between humoral immune responses to epitope peptides and OS are observed only for the CTL epitopes used, or also for other panels of peptides. Quantity of circulating IgG reactive to these peptides was also discussed.
authors.affiliation: Department of Immunology and Immunotherapy, Kurume University School of Medicine, Japan. pines@med.kurume-u.ac.jp
authors.forename: Satoko
authors.initials: S
authors.lastname: Matsueda
authors.forename: Nobukazu
authors.initials: N
authors.lastname: Komatsu
authors.forename: Kenichi
authors.initials: K
authors.lastname: Kusumoto
authors.forename: Shintaro
authors.initials: S
authors.lastname: Koga
authors.forename: Akira
authors.initials: A
authors.lastname: Yamada
authors.forename: Ryoko
authors.initials: R
authors.lastname: Kuromatsu
authors.forename: Shingo
authors.initials: S
authors.lastname: Yamada
authors.forename: Ritsuko
authors.initials: R
authors.lastname: Seki
authors.forename: Shigeru
authors.initials: S
authors.lastname: Yutani
authors.forename: Shigeki
authors.initials: S
authors.lastname: Shichijo
authors.forename: Takashi
authors.initials: T
authors.lastname: Mine
authors.forename: Takaaki
authors.initials: T
authors.lastname: Fukuda
authors.forename: Takashi
authors.initials: T
authors.lastname: Okamura
authors.forename: Seiya
authors.initials: S
authors.lastname: Okuda
authors.forename: Michio
authors.initials: M
authors.lastname: Sata
authors.forename: Junichi
authors.initials: J
authors.lastname: Honda
authors.forename: Masahide
authors.initials: M
authors.lastname: Kaji
authors.forename: Kyogo
authors.initials: K
authors.lastname: Itoh
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Sasada
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000235:genetics / Q000276:immunology*; D001172:Arthritis, Rheumatoid / Q000235:genetics / Q000276:immunology; D014408:Biomarkers, Tumor / Q000235:genetics / Q000276:immunology*; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000276:immunology* / Q000401:mortality; D018984:Epitopes, T-Lymphocyte / Q000235:genetics / Q000276:immunology*; D005260:Female; D005922:Glomerulonephritis, IGA / Q000235:genetics / Q000276:immunology; D006801:Humans; D056724:Immunity, Humoral; D007074:Immunoglobulin G / Q000235:genetics / Q000276:immunology*; D008113:Liver Neoplasms / Q000235:genetics / Q000276:immunology* / Q000401:mortality; D008297:Male; D008875:Middle Aged; D010455:Peptides / Q000235:genetics / Q000276:immunology; D016019:Survival Analysis; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000473:pathology
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D018984:Epitopes, T-Lymphocyte; D007074:Immunoglobulin G; D010455:Peptides