title: Expression of CD203c on basophils as a marker of immunoglobulin E-mediated (L)-asparaginase allergy.
abstract: Immediate allergy to l-asparaginase (ASP) is a major obstacle in treating lymphoid malignancies. ASP-specific immunoglobulin G (ASP-IgG) has been used as a surrogate marker. Recently, the CD203c-basophil activation test (BAT) was found to be useful in diagnosing IgE-mediated allergies. We compared the diagnostic utility of the CD203c-BAT to that of ASP-IgG levels in determining ASP allergies in children. Eight ASP allergic reactions occurred over 75 ASP treatment courses. The sensitivity, specificity and area under the receiver operating characteristic curve of CD203c-BAT were similar to the ASP-IgG levels (0.75 vs. 0.85, 0.82 vs. 0.78 and 0.81 vs. 0.85, respectively). Positive skin prick test results in patients with ASP allergy suggested that ASP-IgE was one of the key players in ASP allergy. A combination of the BAT with the ASP-IgG level had the highest specificity (0.95) and positive predictive value (0.62), which permitted us to identify ASP allergy more effectively.
authors.affiliation: Department of Pediatrics, Graduate School of Medicine, Chiba University , Chiba , Japan.
authors.forename: Moeko
authors.initials: M
authors.lastname: Hino
authors.forename: Naoki
authors.initials: N
authors.lastname: Shimojo
authors.forename: Hidemasa
authors.initials: H
authors.lastname: Ochiai
authors.forename: Yuzaburo
authors.initials: Y
authors.lastname: Inoue
authors.forename: Kumiko
authors.initials: K
authors.lastname: Ando
authors.forename: Koji
authors.initials: K
authors.lastname: Chikaraishi
authors.forename: Setsuo
authors.initials: S
authors.lastname: Ota
authors.forename: Yuri
authors.initials: Y
authors.lastname: Okimoto
authors.forename: Shosuke
authors.initials: S
authors.lastname: Sunami
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Nakamura
authors.forename: Reiko
authors.initials: R
authors.lastname: Teshima
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Sato
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Kohno
mesh_terms: D000293:Adolescent; D001215:Asparaginase / Q000276:immunology*; D001491:Basophils / Q000276:immunology* / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000175:diagnosis / Q000276:immunology* / Q000378:metabolism*; D007073:Immunoglobulin E / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007223:Infant; D008223:Lymphoma / Q000150:complications / Q000628:therapy; D008297:Male; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000150:complications / Q000628:therapy; D011755:Pyrophosphatases / Q000378:metabolism*; D012680:Sensitivity and Specificity
chemical_list: D015415:Biomarkers; C427944:ENPP3 protein, human; D007074:Immunoglobulin G; D007073:Immunoglobulin E; D010727:Phosphoric Diester Hydrolases; D001215:Asparaginase; D011755:Pyrophosphatases