title: Construction of long-wavelength fluorescein analogues and their application as fluorescent probes.
abstract: Born to dye: Five fluorescein analogues were synthesised (see scheme). One analogue was found to emit in the NIR region with a high quantum yield, excellent photostability and good permeability. Three derivatives were found to specifically stain mitochondria and one dye responds to thiols with a strong turn-on NIR fluorescence signal and colorimetric change, in vitro and in vivo.
authors.affiliation: State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, Dalian 116025, PR China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Xiong
authors.forename: Fengling
authors.initials: F
authors.lastname: Song
authors.forename: Gengwen
authors.initials: G
authors.lastname: Chen
authors.forename: Wen
authors.initials: W
authors.lastname: Sun
authors.forename: Jingyun
authors.initials: J
authors.lastname: Wang
authors.forename: Pan
authors.initials: P
authors.lastname: Gao
authors.forename: Yukang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Qiao
authors.forename: Wenfang
authors.initials: W
authors.lastname: Li
authors.forename: Shiguo
authors.initials: S
authors.lastname: Sun
authors.forename: Jiangli
authors.initials: J
authors.lastname: Fan
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Peng
mesh_terms: D000818:Animals; D003545:Cysteine / Q000032:analysis* / Q000737:chemistry; D005260:Female; D005452:Fluoresceins / Q000138:chemical synthesis* / Q000737:chemistry; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D015394:Molecular Structure; D019265:Spectroscopy, Near-Infrared; D013438:Sulfhydryl Compounds; D014407:Tumor Cells, Cultured
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; D013438:Sulfhydryl Compounds; C037631:2',7'-dichlorofluorescein; D003545:Cysteine