title: [Measurement of S-adenosylmethionine and S-adenosylhomocysteine in plasma with high performance liquid chromatography].
abstract: OBJECTIVE
  To develop a method for determination of S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) simultaneously in plasma by reversed-phase high performance liquid chromatography (HPLC).
  
  
  METHODS
  The plasma were hydrolyzed with trichloroacetic acid 400 g/L, then the hydrolysate was adjusted to centrifuged and filtrated with a 0.45 microm membrane. The supernatants were separated on Venusil MP-C18 column (4.6 mm x 250 mm, 5 microm) at 30 degrees C with a mobile phase of 50 mmol/L NaH2PO4 (pH 4.38), C7 H15, NaO3 S and methanol, a flow rate of 1.0 ml/ min, and UV detection at 254nm.
  
  
  RESULTS
  The SAM and SAH in the corresponding concentration range showed a good linear relation with its peak area, correlation coefficient r > 0.9999, recovery was 94.14% - 102.44%, RSD was 1.00% - 8.10%. The content of SAM and SAH in plasma of healthy people was 0.032 - 0.080 mg/L and 0.004 - 0.010 mg/L respectively.
  
  
  CONCLUSIONS
  The validated method is simple, rapid accurate and reliable to the determination of SAM and SAH in plasma.
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Tianjin Medical University, Tianjin 300070, China. lfzsj4523180@163.com
authors.forename: Shijing
authors.initials: S
authors.lastname: Zhao
authors.forename: Na
authors.initials: N
authors.lastname: Sai
authors.forename: Dalin
authors.initials: D
authors.lastname: Ren
authors.forename: Bei
authors.initials: B
authors.lastname: Xu
authors.forename: Chuan
authors.initials: C
authors.lastname: Liu
authors.forename: Guowei
authors.initials: G
authors.lastname: Huang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D012435:S-Adenosylhomocysteine / Q000097:blood*; D012436:S-Adenosylmethionine / Q000097:blood*; D012680:Sensitivity and Specificity
chemical_list: D012436:S-Adenosylmethionine; D012435:S-Adenosylhomocysteine