title: Biological control of Aeromonas salmonicida subsp. salmonicida infection in rainbow trout (Oncorhynchus mykiss) using Aeromonas phage PAS-1.
abstract: The potential control efficacy of Aeromonas phage PAS-1 was evaluated against Aeromonas salmonicida subsp. salmonicida infection in rainbow trout (Oncorhynchus mykiss) model in this study. The phage was co-cultured with the virulent A. salmonicida subsp. salmonicida strain AS05 that possesses the type III secretion system (TTSS) ascV gene, and efficient bacteriolytic activity was observed against the bacteria. The administration of PAS-1 in rainbow trout demonstrated that the phage was cleared from the fish within 200 h post-administration, and a temporal neutralizing activity against the phage was detected in the sera of phage-administrated fish. The administration of PAS-1 (multiplicity of infection: 10 000) in A. salmonicida subsp. salmonicida infected rainbow trout model showed notable protective effects, with increased survival rates and mean times to death. These results demonstrated that Aeromonas phage PAS-1 could be considered as an alternative biological control agent against A. salmonicida subsp. salmonicida infections in rainbow trout culture.
authors.affiliation: Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, Korea; Korea Institute of Ocean Science & Technology, Ansan, Korea.
authors.forename: J H
authors.initials: JH
authors.lastname: Kim
authors.forename: C H
authors.initials: CH
authors.lastname: Choresca
authors.forename: S P
authors.initials: SP
authors.lastname: Shin
authors.forename: J E
authors.initials: JE
authors.lastname: Han
authors.forename: J W
authors.initials: JW
authors.lastname: Jun
authors.forename: S C
authors.initials: SC
authors.lastname: Park
mesh_terms: D048409:Aeromonas salmonicida / Q000472:pathogenicity / Q000821:virology*; D000818:Animals; D017756:Aquaculture / Q000379:methods; D001435:Bacteriophages; D061046:Biological Control Agents; D005393:Fish Diseases / Q000382:microbiology* / Q000517:prevention & control*; D016905:Gram-Negative Bacterial Infections / Q000517:prevention & control / Q000662:veterinary*; D009500:Neutralization Tests / Q000662:veterinary; D017686:Oncorhynchus mykiss; D015996:Survival Rate; D013997:Time Factors
chemical_list: D061046:Biological Control Agents
keywords: Aeromonas phage PAS-1; Aeromonas salmonicida subsp. salmonicida; biological control; rainbow trout (Oncorhynchus mykiss)