title: Enhanced lipid productivity and photosynthesis efficiency in a Desmodesmus sp. mutant induced by heavy carbon ions.
abstract: The unicellular green microalga Desmodesmus sp. S1 can produce more than 50% total lipid of cell dry weight under high light and nitrogen-limitation conditions. After irradiation by heavy (12)C(6+) ion beam of 10, 30, 60, 90 or 120 Gy, followed by screening of resulting mutants on 24-well microplates, more than 500 mutants were obtained. One of those, named D90G-19, exhibited lipid productivity of 0.298 g L(-1)⋅d(-1), 20.6% higher than wild type, likely owing to an improved maximum quantum efficiency (Fv/Fm) of photosynthesis under stress. This work demonstrated that heavy-ion irradiation combined with high-throughput screening is an effective means for trait improvement. The resulting mutant D90G-19 may be used for enhanced lipid production.
authors.affiliation: Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, PR China.
authors.forename: Guangrong
authors.initials: G
authors.lastname: Hu
authors.forename: Yong
authors.initials: Y
authors.lastname: Fan
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Cheng
authors.initials: C
authors.lastname: Yuan
authors.forename: Jufang
authors.initials: J
authors.lastname: Wang
authors.forename: Wenjian
authors.initials: W
authors.lastname: Li
authors.forename: Qiang
authors.initials: Q
authors.lastname: Hu
authors.forename: Fuli
authors.initials: F
authors.lastname: Li
mesh_terms: D018533:Biomass; D002244:Carbon / Q000378:metabolism*; D002470:Cell Survival / Q000235:genetics / Q000528:radiation effects; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D005804:Genes, Lethal; D020450:Heavy Ions; D008055:Lipids / Q000096:biosynthesis* / Q000737:chemistry; D058086:Microalgae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D009154:Mutation; D059645:Mutation Rate; D010641:Phenotype; D010788:Photosynthesis; D013213:Starch / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D008055:Lipids; D002244:Carbon; D013213:Starch