title: High MRI performance fluorescent mesoporous silica-coated magnetic nanoparticles for tracking neural progenitor cells in an ischemic mouse model.
abstract: Multifunctional probes with high MRI sensitivity and high efficiency for cell labeling are desirable for MR cell imaging. Herein, we have fabricated fluorescent mesoporous silica-coated superparamagnetic iron oxide nanoparticles (fmSiO4@SPIONs) for neural progenitor cell (C17.2) MR imaging. FmSiO4@SPIONs were discrete and uniform in size, and had a clear core-shell structure. The magnetic core size was about 10 nm and the fluorescent mesoporous silica coating layer was around 20 nm. Compared with fluorescent dense silica-coated SPIONs (fdSiO4@SPIONs) with a similar size, fmSiO4@SPIONs demonstrated higher MR sensitivity and cell labeling efficiency. When implanted into the right hemisphere of stroke mice, contralateral to the ischemic territory, a small amount of labeled cells were able to be tracked migrating to the lesion sites using a clinical MRI scanner (3 T). More impressively, even when administered intravenously, the labeled cells could also be monitored homing to the ischemic area. MRI observations were corroborated by histological studies of the brain tissues. Our study demonstrated that fmSiO4@SPIONs are highly effective for cell imaging and hold great promise for MRI cell tracking in future.
authors.affiliation: School of Biomedical Engineering & Med-X Research Institute, Shanghai Jiao Tong University, Shanghai 200030, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.forename: Yao
authors.initials: Y
authors.lastname: Wang
authors.forename: Yaohui
authors.initials: Y
authors.lastname: Tang
authors.forename: Zheng
authors.initials: Z
authors.lastname: Jiao
authors.forename: Chengying
authors.initials: C
authors.lastname: Xie
authors.forename: Haijiao
authors.initials: H
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Gu
authors.forename: Xunbin
authors.initials: X
authors.lastname: Wei
authors.forename: Guo-Yuan
authors.initials: GY
authors.lastname: Yang
authors.forename: Hongchen
authors.initials: H
authors.lastname: Gu
authors.forename: Chunfu
authors.initials: C
authors.lastname: Zhang
mesh_terms: D064591:Allografts; D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging* / Q000378:metabolism; D002545:Brain Ischemia / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000628:therapy; D002461:Cell Line, Transformed; D002465:Cell Movement; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D004195:Disease Models, Animal; D005260:Female; D005453:Fluorescence; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D051379:Mice; D058953:Neural Stem Cells / Q000378:metabolism / Q000637:transplantation*; D011859:Radiography; D012822:Silicon Dioxide / Q000737:chemistry / Q000494:pharmacology; D033581:Stem Cell Transplantation
chemical_list: D020099:Coated Materials, Biocompatible; D058185:Magnetite Nanoparticles; D012822:Silicon Dioxide