title: Palbinone from Paeonia suffruticosa protects hepatic cells via up-regulation of heme oxygenase-1.
abstract: Paeonia suffruticosa has been traditionally employed for vitalizing blood circulation and alleviating liver and inflammatory diseases. The pathways by which palbinone (PB) isolated from P. suffruticosa mediates heme oxygenase-1 (HO-1) induction were investigated using the specific inhibitors for PI3K and mitogen activated protein kinases pathways. The effect of PB-treatment on Nrf2 translocalization and HO-1-antioxidant response element (ARE) regulation was examined employing Western blot and luciferase assays. PB induced HO-1 expression via the activation of Nrf2 in the hepatic cells, and ARE-dependent genes were stimulated via the PB-mediated Nrf2 activation. PB-mediated HO-1 expression could be involved with PI3K/Akt and ERK1/2 pathways. Our study suggests the mechanism by which PB induces HO-1 expression in the hepatic cells. This might substantiate the traditional applications of P. suffruticosa for the treatment of oxidative stress-related diseases including oxidant and inflammatory-mediated vascular and liver diseases.
authors.affiliation: National Institute of Medicinal Materials, 3B QuangTrung, Hoankiem, Hanoi, Vietnam; College of Pharmacy, Chungnam National University, Daejeon, 305-764, Republic of Korea.
authors.forename: Do Thi
authors.initials: do T
authors.lastname: Ha
authors.forename: Tran Thi
authors.initials: TT
authors.lastname: Phuong
authors.forename: Joonseok
authors.initials: J
authors.lastname: Oh
authors.forename: Kihwan
authors.initials: K
authors.lastname: Bae
authors.forename: Nguyen Duy
authors.initials: ND
authors.lastname: Thuan
authors.forename: Minkyun
authors.initials: M
authors.lastname: Na
mesh_terms: D061745:Antioxidant Response Elements; D002460:Cell Line; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism*; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors; D051267:NF-E2-Related Factor 2 / Q000235:genetics / Q000378:metabolism; D018384:Oxidative Stress; D029596:Paeonia / Q000737:chemistry*; D000081082:Phosphoinositide-3 Kinase Inhibitors; D018517:Plant Roots / Q000737:chemistry; D051057:Proto-Oncogene Proteins c-akt / Q000037:antagonists & inhibitors; D015398:Signal Transduction / Q000187:drug effects*; D013729:Terpenes / Q000494:pharmacology*; D015533:Transcriptional Activation; D015854:Up-Regulation
chemical_list: D051267:NF-E2-Related Factor 2; C495635:NFE2L2 protein, human; D000081082:Phosphoinositide-3 Kinase Inhibitors; D013729:Terpenes; C080910:palbinone; C496541:HMOX1 protein, human; D051547:Heme Oxygenase-1; D051057:Proto-Oncogene Proteins c-akt; D020928:Mitogen-Activated Protein Kinases
keywords: ERK1/2 pathways; Nrf2 activation; PI3K/Akt pathways; Paeonia suffruticosa; heme oxygenase-1; palbinone