title: [Method establishment for the determination of 6 organophosphorus pesticides metabolites in human urine using LC-MS/MS].
abstract: OBJECTIVE
  To establish a method to determine the presence and concentration of DEP, DMDTP, DEDTP, PNP, CMHC and TCPY organophosphorus pesticides metabolites in human urine by LC-MS/MS.
  
  
  METHODS
  2.0 ml of urine was enzymatically hydrolyzed in water bath 37 degrees C for 4 hours, then the urine samples were solid-phase extracted by Oasis HLB and eluted with methanol, after centrifugation and concentration the urine samples were separated on waters symmetry C18 5 microm (2.1 mm x 150 mm) column with ammonium acetate buffer solution acetonitrile-water as mobile phase to constant gradient elution. Electrospray ionization source (ESI) was applied and operated in positive ion mode, and multiple reaction monitoring (MRM) mode was adopted.
  
  
  RESULTS
  Within concentration ranging from 0 - 1000 ng/ml the correlation coefficient for 5 compounds of 6 were greater than 0.990, recoveries were between 79% - 130%, RSD < 8.2%. The RSD of intra-day and inter-day were less than 15% (n = 6).
  
  
  CONCLUSIONS
  The procedure of samples collection is simple. This is a accurate and specific method for testing organophosphorus pesticides metabolites in human urine.
authors.affiliation: Institute for Environmental Health and Related Product Safety, Chinese CDC, Beijing 100021, China. meijunzhou77@hotmail.com
authors.forename: Zhirong
authors.initials: Z
authors.lastname: Zhou
authors.forename: Miao
authors.initials: M
authors.lastname: Zhang
authors.forename: Shaobin
authors.initials: S
authors.lastname: Lin
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D016273:Occupational Exposure / Q000032:analysis; D009943:Organophosphorus Compounds / Q000652:urine*; D010573:Pesticide Residues / Q000378:metabolism / Q000652:urine*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D009943:Organophosphorus Compounds; D010573:Pesticide Residues