title: Simultaneous quantitation of seven alkaloids in processed Fuzi decoction by rapid resolution liquid chromatography coupled with tandem mass spectrometry.
abstract: A rapid analytical method based on rapid resolution LC coupled with MS/MS was first established to quantify seven alkaloids in processed Fuzi decoction. The chromatographic method was optimized to allow simultaneous analysis of all analytes in 5 min and demonstrated good linearity (r > 0.9995), repeatability (RSD < 4.36%), intra- and interday precisions (RSD < 5.07%) with good accuracies (97.76-105.08%) and good recovery (95.0-107.5%) of seven alkaloids, namely higenamine, benzoylhypaconine, benzoylmesaconine, benzoylaconine, aconitine, hypaconitine, and mesaconitine. The LODs for these markers were in the range of 2.30-17.00 pg/mL. Quantitative analysis of the seven alkaloids in Baifupian decoction and Heishunpian decoction showed that the content of the seven marker chemicals varied significantly and concluded that the quality of Fuzi was greatly affected by different processed methods. The developed method could be used as a rapid, sensitive, and reliable approach for assessment of the quality of processed Fuzi and related decoction.
authors.affiliation: Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing, China. guona5246@126.com
authors.forename: Na
authors.initials: N
authors.lastname: Guo
authors.forename: Dawei
authors.initials: D
authors.lastname: Yang
authors.forename: Keyume
authors.initials: K
authors.lastname: Ablajan
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Niu
authors.forename: Bin
authors.initials: B
authors.lastname: Fan
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Wang
authors.forename: Jingang
authors.initials: J
authors.lastname: Dai
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Wu
authors.forename: Baidong
authors.initials: B
authors.lastname: Liu
mesh_terms: D000470:Alkaloids / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000470:Alkaloids; D004365:Drugs, Chinese Herbal