title: CXCR1/CXCR2 antagonism is effective in pulmonary defense against Klebsiella pneumoniae infection.
abstract: Klebsiella pneumoniae-associated pathology is largely mediated by neutrophilic inflammation. In this study, we administered Klebsiella pneumoniae to experimental guinea pig groups and the ELR-CXC chemokine antagonist CXCL8(3-72), ceftazidime, and dexamethasone to different groups, respectively. After 24 h, we assessed the animal's pulmonary inflammatory levels, including gross histopathology, airway neutrophilia, lung myeloperoxidase levels, expressions of CXCL8 and TNF, and airway bacterial loads. Compared with ceftazidime and dexamethasone treatments, the administration of the ELR-CXC chemokine antagonist CXCL8(3-72) alone was more effective than other methods, although it did not markedly attenuate the bacterial load. These results suggest new methods for the treatment of Klebsiella pneumoniae pathology.
authors.affiliation: Department of Immunology, Dalian Medical University, Dalian 116044, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wei
authors.forename: Jing
authors.initials: J
authors.lastname: Peng
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Qu
authors.forename: Shiyi
authors.initials: S
authors.lastname: Wang
authors.forename: Aziz
authors.initials: A
authors.lastname: Faisal
authors.forename: Jia-Wei
authors.initials: JW
authors.lastname: Cheng
authors.forename: John R
authors.initials: JR
authors.lastname: Gordon
authors.forename: Fang
authors.initials: F
authors.lastname: Li
mesh_terms: D000818:Animals; D002442:Ceftazidime / Q000008:administration & dosage; D003907:Dexamethasone / Q000008:administration & dosage; D006168:Guinea Pigs; D006801:Humans; D016209:Interleukin-8 / Q000037:antagonists & inhibitors / Q000378:metabolism; D007710:Klebsiella Infections / Q000188:drug therapy / Q000382:microbiology / Q000473:pathology; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000472:pathogenicity; D008168:Lung / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D011014:Pneumonia / Q000378:metabolism / Q000473:pathology; D023062:Receptors, Interleukin-8A / Q000037:antagonists & inhibitors*; D023063:Receptors, Interleukin-8B / Q000037:antagonists & inhibitors*
chemical_list: D016209:Interleukin-8; D023062:Receptors, Interleukin-8A; D023063:Receptors, Interleukin-8B; D003907:Dexamethasone; D002442:Ceftazidime