title: Metabolic profiling of phenolic acids and oxidative stress markers after consumption of Lonicera caerulea L. fruit.
abstract: This study investigated the effect of one-week consumption of 165 g/day fresh blue honeysuckle berries (208 mg/day anthocyanins) in 10 healthy volunteers. At the end of intervention, levels of benzoic (median 1782 vs 4156), protocatechuic (709 vs 2417), vanillic (2779 vs 4753), 3-hydroxycinnamic (143 vs 351), p-coumaric (182 vs 271), isoferulic (805 vs 1570), ferulic (1086 vs 2395), and hippuric (194833 vs 398711 μg/mg creatinine) acids by LC/MS were significantly increased in the urine. Clinical chemistry safety markers were not altered. Oxidative stress markers, erythrocyte glutathione peroxidase (0.73 vs 0.88 U/g Hb) and catalase (2.5 vs 2.8 μkat/g Hb) activities, and erythrocyte/plasma thiobarbituric acid reactive substance (522 vs 612/33 vs 38 μmol/g Hb/protein) levels were significantly increased, without change in plasma antioxidant status. Nonsignificant changes of advanced oxidation protein products and oxidized LDL were observed. The results provide a solid base for further study of metabolite excretion and antioxidant parameters after ingestion of anthocyanins.
authors.affiliation: Department of Medical Chemistry and Biochemistry, Palacký University, Hněvotínská 3, 775 15 Olomouc, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Heinrich
authors.forename: Kateřina
authors.initials: K
authors.lastname: Valentová
authors.forename: Jan
authors.initials: J
authors.lastname: Vacek
authors.forename: Irena
authors.initials: I
authors.lastname: Palíková
authors.forename: Martina
authors.initials: M
authors.lastname: Zatloukalová
authors.forename: Pavel
authors.initials: P
authors.lastname: Kosina
authors.forename: Jitka
authors.initials: J
authors.lastname: Ulrichová
authors.forename: Jana
authors.initials: J
authors.lastname: Vrbková
authors.forename: Vilím
authors.initials: V
authors.lastname: Šimánek
mesh_terms: D000328:Adult; D000872:Anthocyanins / Q000008:administration & dosage; D000975:Antioxidants / Q000378:metabolism; D019817:Benzoic Acid / Q000652:urine; D015415:Biomarkers / Q000652:urine*; D002374:Catalase / Q000097:blood; D002853:Chromatography, Liquid; D002934:Cinnamates / Q000652:urine; D003373:Coumaric Acids / Q000652:urine; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D005638:Fruit / Q000737:chemistry*; D005979:Glutathione Peroxidase / Q000097:blood; D006626:Hippurates / Q000652:urine; D006801:Humans; D062385:Hydroxybenzoates / Q000652:urine*; D008077:Lipoproteins, LDL / Q000097:blood; D031239:Lonicera / Q000737:chemistry*; D008297:Male; D013058:Mass Spectrometry; D055442:Metabolome; D018384:Oxidative Stress / Q000187:drug effects*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014641:Vanillic Acid / Q000652:urine
chemical_list: D000872:Anthocyanins; D000975:Antioxidants; D015415:Biomarkers; D002934:Cinnamates; D003373:Coumaric Acids; D006626:Hippurates; D062385:Hydroxybenzoates; D008077:Lipoproteins, LDL; D017392:Thiobarbituric Acid Reactive Substances; C099252:oxidized low density lipoprotein; C009091:protocatechuic acid; D019817:Benzoic Acid; D002374:Catalase; D005979:Glutathione Peroxidase; D014641:Vanillic Acid; C017616:phenolic acid; C030514:hippuric acid; C008180:isoferulic acid