title: In vitro antibacterial activity of Hibiscus rosa-sinensis flower extract against human pathogens.
abstract: OBJECTIVE
  To access the in vitro antibacterial activity of Hibiscus rosa-sinensis (H. rosa- sinensis) flower extract against human pathogens.
  
  
  METHODS
  Antibacterial activity was evaluated by using disc and agar diffusion methods. The protein was run through poly acrylmide gel electrophoresis to view their protein profile.
  
  
  RESULTS
  The results showed that the cold extraction illustrates a maximum zone of inhibition against Bacillus subtillis (B. subtillis), Escherichia coli (E. coli) viz., (17.00 ± 2.91), (14.50 ± 1.71) mm, followed by hot extraction against, E. coli, Salmonella sp. as (11.66 ± 3.14), (10.60 ± 3.09) mm. In methanol extraction showed a highest zone of inhibition recorded against B. subtillis, E. coli as (18.86 ± 0.18), (18.00 ± 1.63) mm pursued by ethanol extraction showed utmost zone of inhibition recorded against Salmonella sp. at (20.40 ± 1.54) mm. The crude protein from flower showed a maximum inhibitory zone observed against Salmonella sp., E. coli viz., (16.55 ± 1.16), (14.30 ± 2.86) mm. The flower material can be taken as an alternative source of antibacterial agent against the human pathogens.
  
  
  CONCLUSIONS
  The extracts of the H. rosa-sinensis are proved to have potential antibacterial activity, further studies are highly need for the drug development.
authors.affiliation: School of Marine Sciences, Department of Oceanography and Coastal Area Studies, Alagappa University, Thondi Campus- 623 409, Tamil Nadu, India.
authors.forename: P
authors.initials: P
authors.lastname: Ruban
authors.forename: K
authors.initials: K
authors.lastname: Gajalakshmi
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000187:drug effects*; D035264:Flowers / Q000737:chemistry*; D031584:Hibiscus / Q000737:chemistry*; D006801:Humans; D066298:In Vitro Techniques; D010936:Plant Extracts / Q000494:pharmacology*; D027826:Rosa / Q000737:chemistry; D012475:Salmonella / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D010936:Plant Extracts
keywords: Antibacterial activity; Hibiscus rosa- sinensis; Human pathogen; Protein; in-vitro