title: Vangl2, the planar cell polarity protein, is complexed with postsynaptic density protein PSD-95 [corrected].
abstract: Vangl is a component of the non-canonical Wnt/planar cell polarity pathway, which is implicated in various cell polarity functions. However, little is known about its synaptic localization in neurons. Here, we show that Vangl1 and Vangl2 are expressed in adult rat neurons, where they are tightly associated with the postsynaptic density (PSD) fraction. Vangl2 forms a complex with PSD-95 through direct binding. Furthermore, the C-terminal PDZ-binding motif of Vangl2 is required for localization to dendritic spines. These results suggest that Vangl2 is a new component of the PSD that forms a complex with PSD-95 in the adult brain.
authors.affiliation: Department of Biochemistry, Faculty of Medicine/Graduate School of Medicine, University of Yamanashi, Japan.
authors.forename: Toshinori
authors.initials: T
authors.lastname: Yoshioka
authors.forename: Akari
authors.initials: A
authors.lastname: Hagiwara
authors.forename: Yamato
authors.initials: Y
authors.lastname: Hida
authors.forename: Toshihisa
authors.initials: T
authors.lastname: Ohtsuka
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism; D016764:Cell Polarity / Q000235:genetics; D002478:Cells, Cultured; D000076122:Disks Large Homolog 4 Protein; D006168:Guinea Pigs; D057809:HEK293 Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008954:Models, Biological; D046912:Multiprotein Complexes / Q000235:genetics / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D057907:Post-Synaptic Density / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding / Q000235:genetics; D051381:Rats; D014162:Transfection
chemical_list: D000076122:Disks Large Homolog 4 Protein; C501449:Dlg4 protein, rat; D047908:Intracellular Signaling Peptides and Proteins; C431577:Ltap protein, mouse; D008565:Membrane Proteins; D046912:Multiprotein Complexes; D009419:Nerve Tissue Proteins