title: Chemical structure of the O-polysaccharides isolated from Cronobacter turicensis sequence type 5 strains 57, 564, and 566.
abstract: The Cronobacter spp. are Gram-negative bacterial pathogens that can cause infections in all age groups, and have a high mortality rate in neonates due to necrotizing enterocolitis and meningitis. Recent genotyping studies have revealed a strong clonal lineage in the genus, but this has not been compared with physiological traits. The O-polysaccharides (OPS) were isolated from three C. turicensis sequence type 5 strains (57, 564, and 566) and structurally characterized using (1)H and (13)C NMR spectroscopy, including two-dimensional DQF-COSY, TOCSY, ROESY, and HSQC analysis. Further compositional determination was undertaken using classical chemical methods followed by GLC, and GLC-MS analysis. The repeating unit of the isolated O-polysaccharides consists of GlcNAc, Rha, Glc, and had the structure shown below and therefore complemented the sequence type. [structure: see text].
authors.affiliation: Faculty of Chemistry, University of Gdansk, Gdansk, Poland. margo@chem.univ.gda.pl
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Czerwicka
authors.forename: Kinga
authors.initials: K
authors.lastname: Marszewska
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Forsythe
authors.forename: Anna
authors.initials: A
authors.lastname: Bychowska
authors.forename: Alicja
authors.initials: A
authors.lastname: Mazgajczyk
authors.forename: Halina
authors.initials: H
authors.lastname: Dziadziuszko
authors.forename: Karolina
authors.initials: K
authors.lastname: Ossowska
authors.forename: Piotr
authors.initials: P
authors.lastname: Stepnowski
authors.forename: Zbigniew
authors.initials: Z
authors.lastname: Kaczyński
mesh_terms: D002240:Carbohydrate Sequence; D059126:Cronobacter / Q000737:chemistry* / Q000235:genetics; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D019081:O Antigens; D011134:Polysaccharides