title: Early Endosome Antigen 1 (EEA1) decreases in macrophages infected with Paracoccidioides brasiliensis.
abstract: Paracoccidioidomycosis (PCM) is a chronic granulomatous disease caused by the dimorphic fungus Paracoccidioides brasiliensis, endemic in Latin America. P. brasiliensis has been observed in epithelial cells in vivo and in vitro, as well as within the macrophages. The identification of the mechanism by which it survives within the host cell is fertile ground for the discovery of its pathogenesis since this organism has the ability to induce its own endocytosis in epithelial cells and most likely in macrophages. The study of the expression of endocytic proteins pathway and co-localization of microorganisms enable detection of the mechanism by which microorganisms survive within the host cell. The aim of this study was to evaluate the expression of the endocytic protein EEA1 (early endosome antigen 1) in macrophages infected with P. brasiliensis. For detection of EEA1, three different techniques were employed: immunofluorescence, real-time polymerase chain reaction (PCR) and immunoblotting. In the present study, decreased expression of EEA1 as well as the rearrangement of the actin was observed when the fungus was internalized, confirming that the input mechanism of the fungus in macrophages occurs through phagocytosis.
authors.affiliation: * Faculdade de Ciências Farmacêuticas, UNESP - Univ Estadual Paulista , Araraquara , São Paulo.
authors.forename: Aline Raquel
authors.initials: AR
authors.lastname: Voltan
authors.forename: Janaina De Cassia Orlandi
authors.initials: Jde C
authors.lastname: Sardi
authors.forename: Christiane Pienna
authors.initials: CP
authors.lastname: Soares
authors.forename: Marcelo
authors.initials: M
authors.lastname: Pelajo Machado
authors.forename: Ana Marisa
authors.initials: AM
authors.lastname: Fusco Almeida
authors.forename: Maria José Soares
authors.initials: MJ
authors.lastname: Mendes-Giannini
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D005455:Fluorescent Antibody Technique; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D054884:Host-Pathogen Interactions; D015151:Immunoblotting; D008264:Macrophages / Q000737:chemistry* / Q000382:microbiology*; D051379:Mice; D010228:Paracoccidioides / Q000254:growth & development* / Q000276:immunology*; D010587:Phagocytosis; D060888:Real-Time Polymerase Chain Reaction; D033921:Vesicular Transport Proteins / Q000032:analysis* / Q000235:genetics
chemical_list: D000199:Actins; D033921:Vesicular Transport Proteins; C093846:early endosome antigen 1