title: M-ds-P21 induces cell apoptosis in bladder cancer T24 cells through P53 independent pathway.
abstract: OBJECTIVE
  To investigate the effect of M-ds-P21 on the apoptosis of bladder cancer T24 cells and its potential mechanism.
  
  
  METHODS
  Effect of M-ds-P21 on T24 cells were assessed by cell morphology and Western blot. Apoptosis was quantified by Annexin-V flow-cytometry analysis. To uncover the role of P53 in M-ds-P21-mediated apoptosis of T24 cells, we knocked down P53 before treating cells with M-ds-P21, and then assayed P21 and apoptosis-related protein by Western blot. To uncover the mechanism by which M-ds-P21 played stronger effect than ds-P21, we performed confocal microscope analyses.
  
  
  RESULTS
  Both M-ds-P21 and ds-P21 treatment changed the cell morphology, leading to cell apoptosis after 3 days. Apoptosis induced by M-ds-P21 and ds-P21 treatment is not P53-dependent but caspase-dependent. Compared with ds-P21, M-ds-P21 significantly increased the bioavailability of ds-RNA in T24 cells.
  
  
  CONCLUSIONS
  M-ds-P21 treatment induces more apoptotic population than ds-P21 does. The mechanism for stronger effect of M-ds-P21 is partly due to the enhanced bioavailability of ds-RNA in human bladder cancer T24 cells, and not P53-dependent but caspase-dependent.
authors.affiliation: Department of Urology, Peking University First Hospital and Institute of Urology, Peking University, National Research Center for Genitourinary Oncology, Beijing, China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Wang
authors.forename: Wujiang
authors.initials: W
authors.lastname: Liu
authors.forename: Jie
authors.initials: J
authors.lastname: Jin
authors.forename: Liqun
authors.initials: L
authors.lastname: Zhou
authors.forename: Lili
authors.initials: L
authors.lastname: Liang
authors.forename: Yinglu
authors.initials: Y
authors.lastname: Guo
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D020029:Base Pairing; D001483:Base Sequence; D020170:Caspase 1 / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D053179:Caspase 7 / Q000378:metabolism; D045744:Cell Line, Tumor; D006801:Humans; D012330:RNA, Double-Stranded / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000378:metabolism*
chemical_list: D012330:RNA, Double-Stranded; D016159:Tumor Suppressor Protein p53; D053148:Caspase 3; D053179:Caspase 7; D020170:Caspase 1