title: Application of meta-analysis methods for identifying proteomic expression level differences.
abstract: We present new statistical approaches for identification of proteins with expression levels that are significantly changed when applying meta-analysis to two or more independent experiments. We showed that the Euclidean distance measure has reduced risk of false positives compared to the rank product method. Our Ψ-ranking method has advantages over the traditional fold-change approach by incorporating both the fold-change direction as well as the p-value. In addition, the second novel method, Π-ranking, considers the ratio of the fold-change and thus integrates all three parameters. We further improved the latter by introducing our third technique, Σ-ranking, which combines all three parameters in a balanced nonparametric approach.
authors.affiliation: Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK.
authors.forename: Bob
authors.initials: B
authors.lastname: Amess
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Kluge
authors.forename: Emanuel
authors.initials: E
authors.lastname: Schwarz
authors.forename: Frieder
authors.initials: F
authors.lastname: Haenisch
authors.forename: Murtada
authors.initials: M
authors.lastname: Alsaif
authors.forename: Robert H
authors.initials: RH
authors.lastname: Yolken
authors.forename: F Markus
authors.initials: FM
authors.lastname: Leweke
authors.forename: Paul C
authors.initials: PC
authors.lastname: Guest
authors.forename: Sabine
authors.initials: S
authors.lastname: Bahn
mesh_terms: D001714:Bipolar Disorder / Q000097:blood; D001798:Blood Proteins / Q000032:analysis / Q000737:chemistry; D016022:Case-Control Studies; D006801:Humans; D007118:Immunoassay; D015201:Meta-Analysis as Topic; D008962:Models, Theoretical; D040901:Proteomics / Q000379:methods*; D012107:Research Design; D018709:Statistics, Nonparametric
chemical_list: D001798:Blood Proteins
keywords: Bipolar disorder; Fold-change; Meta analysis; Statistics; Technology