title: [Effect of diisobutyl phthalate on learning and memory behavior and apoptosis of hippocampus cells in mice].
abstract: OBJECTIVE
  To give the original research of diisobutyl phthalate (DiBP) on learning and memory behavior, determine whether it can through blood-brain barrier and effect apoptosis of hippocampus cells in mice.
  
  
  METHODS
  Accommodating 60 Kunming mice to the animal house for 3 days, then dividing the mice into 5 groups according to their weights. That is, one control group and four experimental groups (I group, 50 mg/kg BW. II group, 250mg/kg BW. III group, 500 mg/kg BW. IV group, 1000 mg/kg BW). The mice were fed with the corn oil in control group, and the other groups were fed with the related dose of diisobutyl phthalate mixture by gavages last for 8 weeks. At the end of experimental time, passive avoidance response was examined, then all of mice were killed, and choosed the brain tissues to test the DiBP content and apoptosis rate of hippocampal cells and hippocampal ultrastructural alterations on electron microscopy.
  
  
  RESULTS
  In the passive avoidance response test, the exposed animals of IV group showed learning impairment as compared to unexposed mice (P < 0.05). DiBP was detected in III group and IV group, the mean content of them were (1.27 +/- 0.56) and (1.96 +/- 0.42) microg/g. The apoptosis rate of hippocampal cells (IV group vs control group) increase significantly (P < 0.05). Hippocampal ultrastructural were damaged in all dose-groups.
  
  
  CONCLUSIONS
  As a result, in the experiments, exposure to DiBP could exert passive avoidance neurobehavioral effects. DiBP could through blood-brain barrier after oral intake, and disordered the way of apoptosis of hippocampal cells, and morphologic change of mitochondria mybe is the main reason of changes of neuron apoptosis.
authors.affiliation: National Center for Food Safety Risk Assessment, Beijing 100021, China. ma_ning34@yahoo.com.cn
authors.forename: Ning
authors.initials: N
authors.lastname: Ma
authors.forename: Shan
authors.initials: S
authors.lastname: Liu
authors.forename: Peng
authors.initials: P
authors.lastname: Gao
authors.forename: Pei
authors.initials: P
authors.lastname: Cao
authors.forename: Haibin
authors.initials: H
authors.lastname: Xu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001362:Avoidance Learning / Q000187:drug effects; D003993:Dibutyl Phthalate / Q000031:analogs & derivatives* / Q000633:toxicity; D004781:Environmental Exposure / Q000009:adverse effects; D006624:Hippocampus / Q000473:pathology*; D007858:Learning / Q000187:drug effects*; D008297:Male; D008568:Memory / Q000187:drug effects*; D051379:Mice
chemical_list: D003993:Dibutyl Phthalate; C025605:diisobutyl phthalate