title: [Effects of weile powder on bicarbonate transporters CFTR SLC26A3 and SLC26A6 in gastric ulcers of rats].
abstract: OBJECTIVE
  To investigate the effects of Weile Powder (WLP) on bicarbonate transporters in rats with gastric ulcers, and to probe its functional mechanisms.
  
  
  METHODS
  The 48 SD rats were randomly divided into the normal control group, the model group, the low dose WLP group (at the daily dose of 0.075 g/mL), the middle dose WLP group (at the daily dose of 0.150 g/mL), the high dose WLP group (at the daily dose of 0.030 g/mL), and the ranitidine group (at the daily dose of 0.030 g/mL), 8 in each group. The gastric ulcer rat model was prepared by the glacial acetic acid cauterization method. Rats in each medication group were administered from the 2nd day of modeling. Rats were sacrificed after 14-day successive medication. The protein was extracted from the ulcer tissue. The protein expressions of solute carrier26A3 (SLC26A3)and solute carrier26A6 (SLC26A6) were detected using Western blot. The gastric ulcer and its peripheral tissue were sectioned. The changes of cystic fibrosis transmembrane conductance regulator (CFTR) were measured by immunofluorescence.
  
  
  RESULTS
  Compared with the model control group, the expression levels of SLC26A3 increased in the high dose WLP group and the ranitidine group with statistical difference (P < 0.05). The expression levels of SLC26A6 increased in the high and middle dose WLP groups and the ranitidine group with statistical difference (P < 0.05). The expression level of CFTR also obviously increased in the high and middle dose WLP groups (P < 0.01).
  
  
  CONCLUSIONS
  WLP could elevate the expression levels of SLC26A6, SLC26A3, and CFTR, increase the secretion of bicarbonate, thus protecting the gastric mucosa.
authors.affiliation: Department of Gastroenterology, Affiliated Hospital, Zunyi Medical College, Guiyang 563000, China.
authors.forename: Guo-Rong
authors.initials: GR
authors.lastname: Wen
authors.forename: Jing-Yu
authors.initials: JY
authors.lastname: Xu
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Liu
authors.forename: Zheng-Lan
authors.initials: ZL
authors.lastname: Zhao
authors.forename: Yi-Xia
authors.initials: YX
authors.lastname: Jiang
authors.forename: Rui
authors.initials: R
authors.lastname: Xie
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.forename: Li-Mei
authors.initials: LM
authors.lastname: Yu
authors.forename: Bi-Guang
authors.initials: BG
authors.lastname: Tuo
mesh_terms: D000818:Animals; D017920:Antiporters / Q000378:metabolism*; D001639:Bicarbonates / Q000378:metabolism; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013276:Stomach Ulcer / Q000378:metabolism*; D000076988:Sulfate Transporters
chemical_list: D017920:Antiporters; D001639:Bicarbonates; D004365:Drugs, Chinese Herbal; C579655:SLC26A6 protein, rat; C489861:Slc26a3 protein, rat; D000076988:Sulfate Transporters; D019005:Cystic Fibrosis Transmembrane Conductance Regulator