title: Influence of reciprocating single-file and rotary instrumentation on bacterial reduction on infected root canals.
abstract: OBJECTIVE
  To compare the bacterial reduction achieved with reciprocating and rotary systems during root canal preparation.
  
  
  METHODS
  Sixty distobuccal root canals of maxillary molars were contaminated with Enterococcus faecalis broth culture. After an incubation period of 21 days, bacterial samples were collected and cultured on m-Enterococcus agar plates. The root canals were divided into five groups, according to the system used for instrumentation: WaveOne, Reciproc, ProTaper, Mtwo and manual instrumentation. The negative controls consisted of five uncontaminated root canals that were subjected to the same instrumentations as each of the experimental groups. Bacterial samples were collected immediately and 7 days after instrumentation. Statistical analysis was performed by paired t-tests and anova tests.
  
  
  RESULTS
  Compared with the samples before instrumentation, the bacterial count was significantly reduced after instrumentation in all groups, with no significant difference in bacterial count reduction amongst the reciprocating, rotary and manual techniques. However, the samples tested 7 days after instrumentation showed significantly higher bacterial counts than the samples tested immediately after instrumentation.
  
  
  CONCLUSIONS
  All systems tested reduced bacterial counts to a similar level.
authors.affiliation: Department of Restorative Dentistry, School of Dentistry, University of São Paulo, São Paulo, SP, Brazil.
authors.forename: M E L
authors.initials: ME
authors.lastname: Machado
authors.forename: C K
authors.initials: CK
authors.lastname: Nabeshima
authors.forename: M F P
authors.initials: MF
authors.lastname: Leonardo
authors.forename: F A S
authors.initials: FA
authors.lastname: Reis
authors.forename: M L B
authors.initials: ML
authors.lastname: Britto
authors.forename: S
authors.initials: S
authors.lastname: Cai
mesh_terms: D001419:Bacteria / Q000302:isolation & purification*; D015169:Colony Count, Microbial; D003786:Dental Pulp Cavity / Q000382:microbiology*; D006801:Humans
keywords: Enterococcus faecalis; endodontics; root canal instrumentation