title: Gallic acid loaded disulfide cross-linked biocompatible polymeric nanogels as controlled release system: synthesis, characterization, and antioxidant activity.
abstract: In this article, a sustained release formulation of the antioxidant gallic acid (GA) is presented in the form of glutathione responsive disulfide cross-linked poly(ethylene glycol)-based nanogels synthesized via aqueous inverse miniemulsion using atom transfer radical polymerization. The particle size was found to be in the range from 227 ± 51.78 to 573.3 ± 207.2 nm at three drug loading levels achieved i.e. 6.6, 14.26, and 18.29 wt.% of the nanogels with loading efficiency in the range of 60-70%. The release profile of the GA studied at three drug loading levels suggested a controlled release and the nanogels were capable of scavenging radicals and retained the antioxidant activity. The GA-loaded nanogels were found to be biocompatible on human cervical cancer cell lines (HeLa). DCFH-DA (2,7-dichlorofluorescin diacetate) assay evidenced that the nanogels were capable of scavenging the reactive oxygen species in cellular environment.
authors.affiliation: Department of Chemistry, Dyal Singh College, University of Delhi, Lodhi Road, Delhi, 110003, India.
authors.forename: Gautam
authors.initials: G
authors.lastname: Behl
authors.forename: Monal
authors.initials: M
authors.lastname: Sharma
authors.forename: Manisha
authors.initials: M
authors.lastname: Sikka
authors.forename: Saurabh
authors.initials: S
authors.lastname: Dahiya
authors.forename: Aruna
authors.initials: A
authors.lastname: Chhikara
authors.forename: Madhu
authors.initials: M
authors.lastname: Chopra
mesh_terms: D000975:Antioxidants / Q000138:chemical synthesis / Q000737:chemistry*; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry*; D001713:Biphenyl Compounds / Q000737:chemistry; D002470:Cell Survival / Q000187:drug effects; D060326:Chemistry Techniques, Synthetic; D003692:Delayed-Action Preparations / Q000737:chemistry; D004220:Disulfides / Q000737:chemistry*; D005707:Gallic Acid / Q000737:chemistry*; D005782:Gels; D005978:Glutathione / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D049329:Nanostructures / Q000737:chemistry*; D010851:Picrates / Q000737:chemistry; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*; D058105:Polymerization; D012995:Solubility; D014867:Water / Q000737:chemistry
chemical_list: D000975:Antioxidants; D001672:Biocompatible Materials; D001713:Biphenyl Compounds; D003692:Delayed-Action Preparations; D004220:Disulfides; D005782:Gels; D010851:Picrates; D014867:Water; D011092:Polyethylene Glycols; D005707:Gallic Acid; C004931:1,1-diphenyl-2-picrylhydrazyl; D005978:Glutathione