title: Antifungal ellagitannin isolated from Euphorbia antisyphilitica Zucc.
abstract: OBJECTIVE
  To study antifungal activity of a new ellagitannin isolated from the plant residues of Euphorbia antisyphilitica (E. antisyphilitica) Zucc in the wax extraction process.
  
  
  METHODS
  An extract was prepared from dehydrated and pulverized residues and fractionated by liquid chromatography on Amberilte XAD-16, until obtained an ellagitannin-rich ethanolic fraction which was treated by rotaevaporation to recover the ellagitannin as fine powder. An aqueous solution was prepared and treated through ionic exchange liquid chromatography (Q XL) and gel permeation chromatography (G 25). The ellagitannin-rich fraction was thermogravimetrically evaluated (TGA and DTA) to test the thermo-stability of ellagic acid (monomeric unit). Then ellagitannin powder was analyzed by infrared spectrospcopy to determinate the functional groups and, also mass spectroscopy was used to determine the molecular ion.
  
  
  RESULTS
  The principal functional groups of ellagitannin were determined, the molecular weight was 860.7 g/mol; and an effective antifungal activity against phytopathogenic fungi was demonstrated.
  
  
  CONCLUSIONS
  It can be concluded that the new ellagitannin (860.7 g/mol) isolated from E. antisyphilitica Zucc is an effective antifungal agent against Alternaria alternata, Fusarium oxyzporum, Colletotrichum gloeosporoides and Rhizoctnia solani.
authors.affiliation: Department of Food Science and Technology, Universidad Autónoma de Coahuila, Saltillo, México.
authors.forename: Juan
authors.initials: J
authors.lastname: Ascacio-Valdés
authors.forename: Edgardo
authors.initials: E
authors.lastname: Burboa
authors.forename: Antonio F
authors.initials: AF
authors.lastname: Aguilera-Carbo
authors.forename: Mario
authors.initials: M
authors.lastname: Aparicio
authors.forename: Ramón
authors.initials: R
authors.lastname: Pérez-Schmidt
authors.forename: Raúl
authors.initials: R
authors.lastname: Rodríguez
authors.forename: Cristóbal N
authors.initials: CN
authors.lastname: Aguilar
mesh_terms: D028481:Euphorbia / Q000737:chemistry*; D005659:Fungicides, Industrial / Q000302:isolation & purification / Q000494:pharmacology*; D047348:Hydrolyzable Tannins / Q000302:isolation & purification / Q000494:pharmacology*; D013058:Mass Spectrometry; D003904:Mitosporic Fungi / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013055:Spectrophotometry, Infrared
chemical_list: D005659:Fungicides, Industrial; D047348:Hydrolyzable Tannins; D010936:Plant Extracts
keywords: Antifungal activity; Ellagitannin; Euphorbia antisyphilitica Zucc; Isolated