title: Staphylococcus aureus supernatant induces the release of mouse β-defensin-14 from osteoblasts via the p38 MAPK and NF-κB pathways.
abstract: Mammalian β-defensins are small cationic peptides of approximately 2-6 kDa that have been implicated in mediating innate immune defenses against microbial infection. Previous studies have reported that mouse β-defensin-14 (MBD‑14), based on structural and functional similarities, appears to be an ortholog of human β-defensin-3 (HBD-3). The aim of this study was to identify the signaling pathways that contribute to the expression of MBD-14 in mouse osteoblasts (OBs) upon contact with methicillin-resistant Staphylococcus aureus (S. aureus) supernatant (SAS) to provide a theoretical basis for the use of MDB-14 as a therapeutic agent in the treatment of intramedullary infection with S. aureus in vivo. The bacterial exoproducts released by S. aureus mainly include a large amount of enterotoxins. Using mouse OBs, the release and regulation of MBD-14 was evaluated by real-time polymerase chain reaction (PCR) and enzyme‑linked immunosorbent assay (ELISA) following exposure to SAS. The activation of the p38 mitogen‑activated protein kinase (MAPK), extracellular signal-regulated kinase (ERK) and nuclear factor-κB (NF-κB) pathways was determined by western blot analysis. OBs treated with lipopolysaccharide (LPS) were used as the positive control. The results revealed that SAS significantly promoted the phosphorylation of p38 MAPK, NF-κB and the inhibitory subunit of NF-κBα (IκBα) in a time-dependent manner. The treatment of OBs with SB203580 (an inhibitor of p38 MAPK) and pyrrolidine dithiocarbamate (PDTC, an inhibitor of NF-κB) prior to stimulation with SAS significantly inhibited the phosphorylation and mRNA expression of p38 MAPK and NF-κB p65, simultaneously reducing the release of MBD-14. Our findings suggest that the release of MBD-14 is mediated at least in part through the activation of p38 MAPK and NF-κB in response to S. aureus‑secreted bacterial exoproducts. Moreover, our data demonstrate the innate immune capacity of OBs under conditions of bacterial challenge to enhance the local expression of this MBD-14, a peptide with anti‑staphylococcal activity.
authors.affiliation: Department of Orthopaedic Surgery, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhu
authors.forename: Hui
authors.initials: H
authors.lastname: Qin
authors.forename: Tao
authors.initials: T
authors.lastname: Cheng
authors.forename: Hong-Lue
authors.initials: HL
authors.lastname: Tan
authors.forename: Yong-Yuan
authors.initials: YY
authors.lastname: Guo
authors.forename: Si-Feng
authors.initials: SF
authors.lastname: Shi
authors.forename: De-Sheng
authors.initials: DS
authors.lastname: Chen
authors.forename: Xian-Long
authors.initials: XL
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism; D018836:Inflammation Mediators / Q000378:metabolism; D051379:Mice; D008826:Microbial Sensitivity Tests; D016328:NF-kappa B / Q000378:metabolism*; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism* / Q000382:microbiology; D015398:Signal Transduction / Q000187:drug effects*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000378:metabolism*; D023083:beta-Defensins / Q000378:metabolism*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D017077:Culture Media, Conditioned; D016207:Cytokines; D018836:Inflammation Mediators; D016328:NF-kappa B; D023083:beta-Defensins; C556378:beta-defensin-14, mouse; D048051:p38 Mitogen-Activated Protein Kinases