title: Overexpression of tumour-associated carbohydrate antigen sialyl-Tn in advanced bladder tumours.
abstract: Little is known on the expression of the tumour-associated carbohydrate antigen sialyl-Tn (STn), in bladder cancer. We report here that 75% of the high-grade bladder tumours, presenting elevated proliferation rates and high risk of recurrence/progression expressed STn. However, it was mainly found in non-proliferative areas of the tumour, namely in cells invading the basal and muscle layers. STn was also found in tumour-adjacent mucosa, which suggests its dependence on a field effect of the tumour. Furthermore, it was not expressed by the normal urothelium, demonstrating the cancer-specific nature of this antigen. STn expression correlated with that of sialyltransferase ST6GalNAc.I, its major biosynthetic enzyme. The stable expression of ST6GalNAc.I in the bladder cancer cell line MCR induced STn expression and a concomitant increase of cell motility and invasive capability. Altogether, these results indicate for the first time a link between STn expression and malignancy in bladder cancer. Hence, therapies targeting STn may constitute new treatment approaches for these tumours.
authors.affiliation: QOPNA, Mass Spectrometry Center, Department of Chemistry, University of Aveiro, Aveiro, Portugal. alexandrecastroferreira@gmail.com
authors.forename: José Alexandre
authors.initials: JA
authors.lastname: Ferreira
authors.forename: Paula A
authors.initials: PA
authors.lastname: Videira
authors.forename: Luís
authors.initials: L
authors.lastname: Lima
authors.forename: Sofia
authors.initials: S
authors.lastname: Pereira
authors.forename: Mariana
authors.initials: M
authors.lastname: Silva
authors.forename: Mylène
authors.initials: M
authors.lastname: Carrascal
authors.forename: Paulo F
authors.initials: PF
authors.lastname: Severino
authors.forename: Elisabete
authors.initials: E
authors.lastname: Fernandes
authors.forename: Andreia
authors.initials: A
authors.lastname: Almeida
authors.forename: Céu
authors.initials: C
authors.lastname: Costa
authors.forename: Rui
authors.initials: R
authors.lastname: Vitorino
authors.forename: Teresina
authors.initials: T
authors.lastname: Amaro
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Oliveira
authors.forename: Celso A
authors.initials: CA
authors.lastname: Reis
authors.forename: Fabio
authors.initials: F
authors.lastname: Dall'Olio
authors.forename: Francisco
authors.initials: F
authors.lastname: Amado
authors.forename: Lúcio Lara
authors.initials: LL
authors.lastname: Santos
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000032:analysis* / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D006801:Humans; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D015854:Up-Regulation; D001743:Urinary Bladder / Q000378:metabolism / Q000473:pathology*; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology*
chemical_list: D015295:Antigens, Tumor-Associated, Carbohydrate; C055491:sialosyl-Tn antigen