title: Structural studies of the O-antigenic polysaccharide of the bovine mastitis isolate Escherichia coli serotype O174.
abstract: Escherichia coli is one of the major causative agents of bovine mastitis, a disease affecting dairy herds. The lipopolysaccharide (LPS) of E. coli was plays a prominent role during infection. Here, we report on the O-antigen chemical structure of the LPS from Escherichia coli strain 2188 (serotype O174:H28) isolated from a mastitis-diseased cow. The structure of the OPS was analyzed by 1 and 2D NMR spectroscopy, and methylation analysis, which identified the branched repeating tetrasaccharide biological unit. [structure: see text].
authors.affiliation: Division of Structural Biochemistry, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Airway Research Center North (ARCN), Borstel, Germany. kduda@fz-borstel.de
authors.forename: Katarzyna A
authors.initials: KA
authors.lastname: Duda
authors.forename: Angelika
authors.initials: A
authors.lastname: Fruth
authors.forename: Otto
authors.initials: O
authors.lastname: Holst
mesh_terms: D000818:Animals; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D002417:Cattle; D004926:Escherichia coli / Q000145:classification* / Q000276:immunology / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary; D005260:Female; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008414:Mastitis, Bovine / Q000382:microbiology*; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry* / Q000276:immunology; D012703:Serotyping
chemical_list: D019081:O Antigens