title: Effects of trans-fatty acids on liver lipid metabolism in mice fed on diets showing different fatty acid composition.
abstract: OBJECTIVE
  Our aim was to investigate the effects of trans-fatty acids (TFA) on liver lipid metabolism in mice fed on experimental diets rich in either oleic or linoleic acid.
  
  
  METHODS
  Twenty-two male CF1 mice (22.0 ± 0.1 g) were fed with diets rich in corn oil or olive oil, supplemented or not with TFA (0.75 g TFA/100 g diet), for 4 weeks. Changes in triacylglycerol content, the activity and expression of enzymes involved in lipogenesis and fatty acid oxidation were measured.
  
  
  RESULTS
  Supplementation of an olive oil-rich diet with TFA increased liver triacylglycerols, the activity and expression of lipogenic enzymes and sterol regulatory element-binding protein SREBP-1a expression. By contrast, when TFA were added to a corn oil-rich diet, they did not modify these parameters. No significant differences were observed among the experimental groups in the activity and expression of carnitine palmitoyltransferase-Ia, body and liver weights or serum triacylglycerol concentrations.
  
  
  CONCLUSIONS
  The effect of TFA on liver fat accumulation depends on the dietary fatty acid composition. Steatosis induced by TFA when included in an olive oil diet (but not in a corn oil diet) was associated with an increased lipogenesis but not with a decreased fatty acid oxidation in animals fed on the olive oil diet. This metabolic change is mediated by SREBP-1a but not by SREBP-1c, and seems to be independent of insulin.
authors.affiliation: Cátedra de Bromatología y Nutrición, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina.
authors.forename: Juliana
authors.initials: J
authors.lastname: Saín
authors.forename: Marcela A
authors.initials: MA
authors.lastname: González
authors.forename: Arrate
authors.initials: A
authors.lastname: Lasa
authors.forename: María Victoria
authors.initials: MV
authors.lastname: Scalerandi
authors.forename: Claudio A
authors.initials: CA
authors.lastname: Bernal
authors.forename: Maria P
authors.initials: MP
authors.lastname: Portillo
mesh_terms: D000818:Animals; D003314:Corn Oil / Q000009:adverse effects / Q000378:metabolism; D004041:Dietary Fats / Q000009:adverse effects / Q000378:metabolism*; D005234:Fatty Liver / Q000201:enzymology / Q000209:etiology* / Q000378:metabolism; D005786:Gene Expression Regulation; D006865:Hydrogenation; D019787:Linoleic Acid / Q000009:adverse effects / Q000378:metabolism; D050356:Lipid Metabolism; D050155:Lipogenesis; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D019301:Oleic Acid / Q000009:adverse effects / Q000378:metabolism; D000069463:Olive Oil; D010084:Oxidation-Reduction; D010938:Plant Oils / Q000009:adverse effects / Q000378:metabolism; D011897:Random Allocation; D051780:Sterol Regulatory Element Binding Protein 1 / Q000378:metabolism; D044242:Trans Fatty Acids / Q000009:adverse effects*; D014280:Triglycerides / Q000009:adverse effects / Q000378:metabolism
chemical_list: D004041:Dietary Fats; D000069463:Olive Oil; D010938:Plant Oils; C497388:Srebf1 protein, mouse; D051780:Sterol Regulatory Element Binding Protein 1; D044242:Trans Fatty Acids; D014280:Triglycerides; D019301:Oleic Acid; D003314:Corn Oil; D019787:Linoleic Acid