title: Cloning and characterization of chicken α5 integrin: endogenous and experimental expression in early chicken embryos.
abstract: Key roles for fibronectin and its integrin receptors have been postulated in the multiple cell-matrix interactions essential for chick embryo morphogenesis. However, mechanistic studies of these processes have been hampered by the current absence of sequence data and chicken cDNA clones for the major fibronectin receptor subunit, integrin α5 (ITGA5). We report here the sequence, endogenous expression pattern, and transfection of full-length chicken integrin α5. During early chicken embryonic development, α5 is highly expressed in cranial neural folds and migrating neural crest cells, suggesting potential roles in neural crest formation and migration. In fact, over-expression of this integrin in early neural tube selectively induces BMP5, a growth factor recently implicated in neural crest formation. Availability of these α5 integrin tools should facilitate studies of its functions in early embryonic development.
authors.affiliation: Laboratory of Cell and Developmental Biology, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892-4370, United States. Electronic address: yukinorishiko@yahoo.co.jp.
authors.forename: Yukinori
authors.initials: Y
authors.lastname: Endo
authors.forename: Hiroko
authors.initials: H
authors.lastname: Ishiwata-Endo
authors.forename: Kenneth M
authors.initials: KM
authors.lastname: Yamada
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D055417:Bone Morphogenetic Protein 5 / Q000378:metabolism; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics*; D003001:Cloning, Molecular; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D040461:Gene Components; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D005822:Genetic Vectors / Q000235:genetics; D017403:In Situ Hybridization; D039482:Integrin alpha5 / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D009432:Neural Crest / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D055417:Bone Morphogenetic Protein 5; D017931:DNA Primers; D018076:DNA, Complementary; D039482:Integrin alpha5
keywords: Chicken embryo; Extracellular matrix; Fibronectin; Neural crest; α5 integrin