title: Gene-carried chitosan-linked polyethylenimine induced high gene transfection efficiency on dendritic cells.
abstract: A dendritic cell (DC) networking system has become an attractive approach in cancer immunotherapy. Successful DC gene engineering depends on the development of transgene vectors. A cationic polymer, chitosan-linked polyethylenimine (PEI) (CP), possessing the advantages of both PEI and chitosan, has been applied in nonviral transfection of DCs. Physicochemical evaluation showed that CP/DNA complexes could form cationic nanoparticles. Compared with DCs transfected with commercial reagent, Lipofectamine2000, it showed higher transfection efficiency and lower cytotoxicity when DCs were transfected with CP/DNA complexes. A nuclear trafficking observation of CP/DNA complexes by a confocal laser scanning microscope further revealed that the CP could help DNA enter into the cytoplasm and finally into the nucleus of a DC. Finally, vaccination of DCs transfected with CP/DNA encoding gp100 slightly improved resistance to the B16BL6 melanoma challenge. This is the first report that CP polymer is used as a nonviral vector for DC gene delivery and DC vaccine. Essentially, these results might be helpful to design a promising nonviral vector for DC gene delivery.
authors.affiliation: Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, Zhejiang, People's Republic of China.
authors.forename: Yu-Zhe
authors.initials: YZ
authors.lastname: Chen
authors.forename: Xing-Lei
authors.initials: XL
authors.lastname: Yao
authors.forename: Gui-Xin
authors.initials: GX
authors.lastname: Ruan
authors.forename: Qing-Qing
authors.initials: QQ
authors.lastname: Zhao
authors.forename: Gu-Ping
authors.initials: GP
authors.lastname: Tang
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Tabata
authors.forename: Jian-Qing
authors.initials: JQ
authors.lastname: Gao
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D004247:DNA / Q000235:genetics / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism*; D004337:Drug Carriers / Q000737:chemistry* / Q000633:toxicity; D005822:Genetic Vectors / Q000235:genetics; D008297:Male; D008546:Melanoma, Experimental / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D010957:Plasmids / Q000235:genetics; D011094:Polyethyleneimine / Q000737:chemistry*; D014162:Transfection / Q000379:methods*; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology
chemical_list: D000951:Antigens, Neoplasm; D004337:Drug Carriers; D019444:Vaccines, DNA; D011094:Polyethyleneimine; D004247:DNA; D048271:Chitosan