title: Application of two-phase hollow fiber liquid phase microextraction coupled with high-performance liquid chromatography for the study of the echinacoside pharmacokinetics in Parkinson's disease rat plasma.
abstract: A simple and solvent-minimized sample preparation technique based on two-phase hollow fiber liquid phase microextraction (HF-LPME) was developed and used to quantify the echinacoside in Parkinson's disease rat plasma following oral administration. Analysis was accomplished by reversed-phase high performance liquid chromatography (HPLC) with ultraviolet detection. The influence factors relevant to the HF-LPME processes were optimized. Under the optimized conditions, the preconcentration factor for echinacoside was 337. Calibration curves with reasonable linearity (r(2) ≥ 0.9998) were obtained in the range of 5-750 ng mL(-1). Intra-day and inter-day precision (RSD) were ≤ 5.43% and the limit of detection (LOD) for the analyte was 2.0 ng mL(-1) (S/N=3). The validated method has been successfully applied for pharmacokinetic studies of echinacoside in Parkinson's disease (PD) rat plasma after oral administration.
authors.affiliation: Department of Pharmacy, Urumqi General Hospital of PLA, Urumqi, Xinjiang 830000, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhou
authors.forename: Ping
authors.initials: P
authors.lastname: Zeng
authors.forename: Jiang Bing
authors.initials: JB
authors.lastname: Sun
authors.forename: Feng Qiao
authors.initials: FQ
authors.lastname: Wang
authors.forename: Qiong
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods; D006027:Glycosides / Q000008:administration & dosage / Q000493:pharmacokinetics*; D057230:Limit of Detection; D059627:Liquid Phase Microextraction / Q000379:methods*; D008297:Male; D020734:Parkinsonian Disorders / Q000188:drug therapy* / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D006027:Glycosides; C060297:echinacoside