title: In vivo hepatic lipid quantification using MRS at 7 Tesla in a mouse model of glycogen storage disease type 1a.
abstract: The assessment of liver lipid content and composition is needed in preclinical research to investigate steatosis and steatosis-related disorders. The purpose of this study was to quantify in vivo hepatic fatty acid content and composition using a method based on short echo time proton magnetic resonance spectroscopy (MRS) at 7 Tesla. A mouse model of glycogen storage disease type 1a with inducible liver-specific deletion of the glucose-6-phosphatase gene (L-G6pc(-/-)) mice and control mice were fed a standard diet or a high-fat/high-sucrose (HF/HS) diet for 9 months. In control mice, hepatic lipid content was found significantly higher with the HF/HS diet than with the standard diet. As expected, hepatic lipid content was already elevated in L-G6pc(-/-) mice fed a standard diet compared with control mice. L-G6pc(-/-) mice rapidly developed steatosis which was not modified by the HF/HS diet. On the standard diet, estimated amplitudes from olefinic protons were found significantly higher in L-G6pc(-/-) mice compared with that in control mice. L-G6pc(-/-) mice showed no noticeable polyunsaturation from diallylic protons. Total unsaturated fatty acid indexes measured by gas chromatography were in agreement with MRS measurements. These results showed the great potential of high magnetic field MRS to follow the diet impact and lipid alterations in mouse liver.
authors.affiliation: Université de Lyon, CREATIS, CNRS UMR 5220, Inserm U1044, INSA-Lyon, Université Lyon 1, Villeurbanne, France.
authors.forename: Nirilanto
authors.initials: N
authors.lastname: Ramamonjisoa
authors.forename: Helene
authors.initials: H
authors.lastname: Ratiney
authors.forename: Elodie
authors.initials: E
authors.lastname: Mutel
authors.forename: Herve
authors.initials: H
authors.lastname: Guillou
authors.forename: Gilles
authors.initials: G
authors.lastname: Mithieux
authors.forename: Frank
authors.initials: F
authors.lastname: Pilleul
authors.forename: Fabienne
authors.initials: F
authors.lastname: Rajas
authors.forename: Olivier
authors.initials: O
authors.lastname: Beuf
authors.forename: Sophie
authors.initials: S
authors.lastname: Cavassila
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D005952:Glucose-6-Phosphatase / Q000235:genetics / Q000378:metabolism*; D005953:Glycogen Storage Disease Type I / Q000201:enzymology / Q000378:metabolism*; D008055:Lipids / Q000032:analysis*; D008099:Liver / Q000737:chemistry* / Q000378:metabolism; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D011522:Protons
chemical_list: D008055:Lipids; D011522:Protons; D005952:Glucose-6-Phosphatase
keywords: hepatic fatty acid quantification and composition; magnetic resonance spectroscopy; steatosis