title: Simultaneous miRNA and mRNA transcriptome profiling of human myoblasts reveals a novel set of myogenic differentiation-associated miRNAs and their target genes.
abstract: BACKGROUND
  miRNA profiling performed in myogenic cells and biopsies from skeletal muscles has previously identified miRNAs involved in myogenesis.
  
  
  RESULTS
  Here, we have performed miRNA transcriptome profiling in human affinity-purified CD56+ myoblasts induced to differentiate in vitro. In total, we have identified 60 miRNAs differentially expressed during myogenic differentiation. Many were not known for being differentially expressed during myogenic differentiation. Of these, 14 (miR-23b, miR-28, miR-98, miR-103, miR-107, miR-193a, miR-210, miR-324-5p, miR-324-3p, miR-331, miR-374, miR-432, miR-502, and miR-660) were upregulated and 6 (miR-31, miR-451, miR-452, miR-565, miR-594 and miR-659) were downregulated. mRNA transcriptome profiling performed in parallel resulted in identification of 6,616 genes differentially expressed during myogenic differentiation.
  
  
  CONCLUSIONS
  This simultaneous miRNA/mRNA transcriptome profiling allowed us to predict with high accuracy target genes of myogenesis-related microRNAs and to deduce their functions.
authors.affiliation: UMR 8126, Univ. Paris-Sud 11, CNRS, Institut de Cancérologie Gustave-Roussy, 39, rue Camille-Desmoulins, Villejuif 94805, France.
authors.forename: Petr
authors.initials: P
authors.lastname: Dmitriev
authors.forename: Ana
authors.initials: A
authors.lastname: Barat
authors.forename: Anna
authors.initials: A
authors.lastname: Polesskaya
authors.forename: Mary J
authors.initials: MJ
authors.lastname: O'Connell
authors.forename: Thomas
authors.initials: T
authors.lastname: Robert
authors.forename: Philippe
authors.initials: P
authors.lastname: Dessen
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Walsh
authors.forename: Vladimir
authors.initials: V
authors.lastname: Lazar
authors.forename: Ahmed
authors.initials: A
authors.lastname: Turki
authors.forename: Gilles
authors.initials: G
authors.lastname: Carnac
authors.forename: Dalila
authors.initials: D
authors.lastname: Laoudj-Chenivesse
authors.forename: Marc
authors.initials: M
authors.lastname: Lipinski
authors.forename: Yegor S
authors.initials: YS
authors.lastname: Vassetzky
mesh_terms: D019002:CD56 Antigen / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics*; D015536:Down-Regulation; D020869:Gene Expression Profiling; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000502:physiology; D024510:Muscle Development / Q000235:genetics*; D018482:Muscle, Skeletal / Q000378:metabolism*; D032446:Myoblasts / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D019002:CD56 Antigen; C544547:MIRN1 microRNA, human; C548234:MIRN133 microRNA, human; C546087:MIRN206 microRNA, human; D035683:MicroRNAs; C522918:NCAM1 protein, human; D012333:RNA, Messenger