title: Real-time fluorometric assay for acetylcholinesterase activity and inhibitor screening through the pyrene probe monomer-excimer transition.
abstract: A choline labeled pyrene probe (Py-Ch) was designed and synthesized. Poly(vinylsulfonate) (PVS) could induce Py-Ch aggregation. The aggregation and deaggregation process could be finely controlled by the acetylcholinesterase (AChE) enzymatic hydrolysis of Py-Ch. The resulting excimer-monomer transition provided a facile way for real-time AChE activity fluorometric assay and inhibitor screening.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.forename: Dongli
authors.initials: D
authors.lastname: Liao
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Huipeng
authors.initials: H
authors.lastname: Zhou
authors.forename: Wenying
authors.initials: W
authors.lastname: Li
authors.forename: Cong
authors.initials: C
authors.lastname: Yu
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology*; D005470:Fluorometry; D015335:Molecular Probes; D015394:Molecular Structure; D011721:Pyrenes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D015335:Molecular Probes; D011721:Pyrenes; D000110:Acetylcholinesterase