title: Expression of secreted frizzled-related protein 1 and 3, T-cell factor 1 and lymphoid enhancer factor 1 in clear cell renal cell carcinoma.
abstract: Frequency and mortality of renal cell carcinoma (RCC) are increasing for decades. However, the molecular background of RCC tumorigenesis is still poorly understood. In current study we investigated the expression of TCF/LEF and SFRP family members (SFRP1 and SFRP3) to gain a better understanding of biological signaling pathways responsible for epidemiology and clinical parameters of clear cell RCC (cRCC). Thirty-six pairs of paraffin-embedded clear cRCC and adjacent nontumoral tissues samples using immunohistochemistry (IHC) were analyzed and compared with corresponding clinicopathological parameters. Immunohistochemistry indicated statistically significant decreased SFRP3 expression in tumor tissues but no consistency in SFRP1 expression in analyzed normal and tumor tissue. The TCF1 expression level was significantly weaker in normal tissue compared to tumor samples while LEF1 protein levels were significantly weaker in tumor tissue. To our knowledge, this is the first report on analysis of the expression of transcription factors TCF1 and LEF1 in clear cell renal cell carcinoma and their comparison with Wnt signal pathway antagonists belonging to SFRP family.
authors.affiliation: Department of Biology, School of Medicine, University of Zagreb, Šalata 3, 10000 Zagreb, Croatia. tmartic@mef.hr
authors.forename: Tamara
authors.initials: T
authors.lastname: Nikuševa-Martić
authors.forename: Ljiljana
authors.initials: L
authors.lastname: Serman
authors.forename: Martina
authors.initials: M
authors.lastname: Zeljko
authors.forename: Zeljko
authors.initials: Z
authors.lastname: Vidas
authors.forename: Slavko
authors.initials: S
authors.lastname: Gašparov
authors.forename: Hrvojka Marija
authors.initials: HM
authors.lastname: Zeljko
authors.forename: Marin
authors.initials: M
authors.lastname: Kosović
authors.forename: Nives
authors.initials: N
authors.lastname: Pećina-Šlaus
mesh_terms: D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D002292:Carcinoma, Renal Cell / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins; D007680:Kidney Neoplasms / Q000378:metabolism* / Q000473:pathology; D051821:Lymphoid Enhancer-Binding Factor 1 / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D025341:Principal Component Analysis; D018709:Statistics, Nonparametric; D051822:T Cell Transcription Factor 1 / Q000378:metabolism*; D000099274:Frizzled-Related Proteins
chemical_list: D006023:Glycoproteins; D047908:Intracellular Signaling Peptides and Proteins; D051821:Lymphoid Enhancer-Binding Factor 1; D051822:T Cell Transcription Factor 1; D000099274:Frizzled-Related Proteins; C497573:LEF1 protein, human; C497576:TCF7 protein, human