title: Determination of neotame in non-alcoholic beverage by capillary zone electrophoresis.
abstract: BACKGROUND
  Neotame (NEO) is a new artificial sweetener approved as a food additive in many countries. The present method for the determination of NEO in various foodstuffs is high-performance liquid chromatography (HPLC). There are no reports on the determination of NEO in foods by capillary zone electrophoresis (CZE). Therefore a simple and rapid method to determine NEO is desired for quality control.
  
  
  RESULTS
  A CZE method combined with solid phase extraction was developed for the determination of NEO in non-alcoholic beverage. The optimum separation conditions obtained were 20 mmol L(-1) sodium borate buffer, pH 8, 25 kV applied voltage, 5 s hydrodynamic injection at 30 mbar and ultraviolet detection at 191 nm. The calibration curve showed good linearity (R(2) = 1.000) in the range 0.5-100 µg mL(-1) , and the limit of detection was 0.118 µg mL(-1) . The method was successfully applied to the determination of NEO in two kinds of beverage with migration time less than 5 min, relative standard deviation (n = 3) less than 2% and recoveries ranging from 90 to 95%.
  
  
  CONCLUSIONS
  The proposed CZE method has the advantages of shorter analysis time and lower cost compared with HPLC, indicating that it may be a good alternative to the HPLC method.
authors.affiliation: College of Chemistry and Chemical Engineering, Yantai University, Yantai, 264005, China.
authors.forename: Falian
authors.initials: F
authors.lastname: Hu
authors.forename: Lina
authors.initials: L
authors.lastname: Xu
authors.forename: Feng
authors.initials: F
authors.lastname: Luan
authors.forename: Huitao
authors.initials: H
authors.lastname: Liu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Gao
mesh_terms: D001628:Beverages / Q000032:analysis*; D002021:Buffers; D002851:Chromatography, High Pressure Liquid; D003365:Costs and Cost Analysis; D004151:Dipeptides / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000191:economics / Q000379:methods*; D005503:Food Additives / Q000032:analysis; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction; D013549:Sweetening Agents / Q000032:analysis*; D013997:Time Factors
chemical_list: D002021:Buffers; D004151:Dipeptides; D005503:Food Additives; D013549:Sweetening Agents; C404525:neotame
keywords: artificial sweeteners; capillary zone electrophoresis; neotame; solid phase extraction