title: Decreased prevalence of celiac disease among Brazilian elderly.
abstract: OBJECTIVE
  To evaluate the prevalence of celiac disease in a group of Brazilian individuals over 60 years of age and compare it with the previously known prevalence in a pediatric group living in the same geographical area.
  
  
  METHODS
  The research protocol was approved by the Ethics Committee of the University of Brasilia School of Medicine, Brasilia, Brazil. Blood samples from 946 individuals (295 male and 651 female) aged 60 years or older were collected between May 2010 and July 2011. The study subjects' mean and median ages were 68.1 and 67 years, respectively, ranging from 60 to 92 years. That age distribution closely corresponded to the age distribution of the Brazilian population according to the Brazilian 2010 census. The participants were consecutive and unselected outpatients undergoing blood tests at the University of Brasilia Hospital's Clinical Pathology Laboratory. All sera were tested for immunoglobulin A anti-transglutaminase antibodies (IgA-tTG) by enzyme- linked immunosorbent assay, and those that were positive were further tested for immunoglobulin A anti-endomysium antibodies (IgA-EMA). Human leukocyte antigen (HLA) genotyping was performed for all individuals who exhibited positive serologic results for IgA-tTG and/or IgA-EMA.
  
  
  RESULTS
  Out of the 946 studied patients, only one previously diagnosed case of biopsy-proven celiac disease was detected. For the remaining subjects, nine serum samples tested positive for IgA-tTG antibodies; however, none of them tested positive for IgA-EMA antibodies. The HLA genotyping of those nine subjects revealed that one was carrying DQA1*0501 and two were carrying DQB1*0201 alleles. These data showed that, among those 946 elderly individuals, the prevalence of celiac disease (CD) was 0.1% (95%CI: 0.00-0.59). The prevalence of CD for the elderly group was compared with that observed for the group of 2034 children younger than 15 years (age range, 1-14 years; mean age, 8 years) who took part in our previous CD prevalence screening study. All the children came from the same geographical region and shared a similar ethnic and low-income background. As in the elderly group in the current study, the younger group was made up of consecutive outpatients who underwent blood evaluation at the University of Brasilia Hospital's Clinical Laboratory. The prevalence of biopsy-proven CD among those children was 0.54% (95%CI: 0.27-0.57). The comparative analysis between the two groups resulted in the following values: odds ratio = 0.19 (95%CI: 0.01-1.45) Fisher test P = 0.06.
  
  
  CONCLUSIONS
  The prevalence of CD among the children of our previous study was 5.4 times higher than that found in the present elderly group.
authors.affiliation: Graduate Program in Medical Sciences, University of Brasilia School of Medicin, Brasilia DF 70910900, Brazil.
authors.forename: Lucas Malta
authors.initials: LM
authors.lastname: Almeida
authors.forename: Luiz Claudio
authors.initials: LC
authors.lastname: Castro
authors.forename: Rosa Harumi
authors.initials: RH
authors.lastname: Uenishi
authors.forename: Fernanda Coutinho
authors.initials: FC
authors.lastname: de Almeida
authors.forename: Patricia Maria
authors.initials: PM
authors.lastname: Fritsch
authors.forename: Lenora
authors.initials: L
authors.lastname: Gandolfi
authors.forename: Riccardo
authors.initials: R
authors.lastname: Pratesi
authors.forename: Yanna Karla de Medeiros
authors.initials: YK
authors.lastname: Nóbrega
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000906:Antibodies / Q000097:blood; D001938:Brazil / Q000453:epidemiology; D002446:Celiac Disease / Q000097:blood / Q000453:epidemiology* / Q000276:immunology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D015995:Prevalence; D011503:Transglutaminases / Q000276:immunology
chemical_list: D000906:Antibodies; D011503:Transglutaminases
keywords: Celiac disease; Elderly; Epidemiology; Gluten-sensitive enteropathy; Mortality