title: MMPREDOX/NO interplay in periodontitis and its inhibition with Satureja hortensis L. essential oil.
abstract: Satureja hortensis L. is an aromatic plant with antibacterial and antibiofilm activities against periodontopathogens. Here, we attempted to find out whether the antioxidant properties of S. hortensis L. essential oil (EO) could be used to inhibit matrix metalloproteinase (MMP) activities and prevent the induction of cell death by a pro-oxidant insult. First, a landscape analysis of MMP and REDOX/nitric oxide (NO)-related genes was performed (MRN model), and array data from periodontitis patients were plotted over the newly developed model. Thereafter, the antigelatinolytic activity of S. hortensis L. EO and its preventive effect against hydrogen peroxide (H2 O2 )-induced cell death were tested in vitro (HaCaT cells). Up-regulation of MMP genes in the MRN network (except for MMP-10, -15, -16, -20, -25, and -26) and differential expression of genes coding for antioxidant enzymes were found among others in periodontitis samples. MMP2 and MMP9 were central genes in the MRN network model. Moreover, treatments with 1 and 5 μl/ml of S. hortensis L. EO inhibited both MMP-2 and MMP-9 activities, and H2 O2 -induced cell death in vitro. We concluded that S. hortensis L. EO could be a promising host-modulating agent, since oxidative stress and excessive MMP expression/activity are typical hallmarks of periodontal pathogenesis.
authors.affiliation: Centro de Estudos em Estresse Oxidativo, Departamento de Bioquímica, ICBS-UFRGS, Rua Ramiro Barcelos 2600 - ANEXO, Porto Alegre, 90035-003, RS, Brasil. fzchulia.biomed@gmail.com
authors.forename: Fares
authors.initials: F
authors.lastname: Zeidán-Chuliá
authors.forename: Ben-Hur
authors.initials: BH
authors.lastname: Neves de Oliveira
authors.forename: Mervi
authors.initials: M
authors.lastname: Gursoy
authors.forename: Eija
authors.initials: E
authors.lastname: Könönen
authors.forename: José Cláudio
authors.initials: JC
authors.lastname: Fonseca Moreira
authors.forename: Ulvi K
authors.initials: UK
authors.lastname: Gursoy
authors.forename: Veli-Jukka
authors.initials: VJ
authors.lastname: Uitto
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D000975:Antioxidants / Q000737:chemistry*; D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D053263:Gene Regulatory Networks; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity; D020778:Matrix Metalloproteinase 2 / Q000737:chemistry / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000737:chemistry / Q000378:metabolism; D061965:Matrix Metalloproteinase Inhibitors / Q000737:chemistry* / Q000494:pharmacology; D020782:Matrix Metalloproteinases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D008958:Models, Molecular; D009569:Nitric Oxide / Q000378:metabolism*; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology; D010084:Oxidation-Reduction; D010518:Periodontitis / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D031362:Satureja / Q000737:chemistry*; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D000975:Antioxidants; D061965:Matrix Metalloproteinase Inhibitors; D009822:Oils, Volatile; D009569:Nitric Oxide; D006861:Hydrogen Peroxide; D020782:Matrix Metalloproteinases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9