title: Real-time optical antimicrobial susceptibility testing.
abstract: Rapid antibiotic susceptibility testing is in high demand in health care fields as antimicrobial-resistant bacterial strains emerge and spread. Here, we describe an optical screening system (oCelloScope) which, based on time-lapse imaging of 96 bacteria-antibiotic combinations at a time, introduces real-time detection of bacterial growth and antimicrobial susceptibility with imaging material to support the automatically generated graphs. Automated antibiotic susceptibility tests of a monoculture showed statistically significant antibiotic effects within 6 min and within 30 min in complex samples from pigs suffering from catheter-associated urinary tract infections. The oCelloScope system provides a fast high-throughput screening method for detecting bacterial susceptibility that might entail an earlier diagnosis and introduction of appropriate targeted therapy and thus combat the threat from multidrug-resistant pathogenic bacteria. The oCelloScope system can be employed for a broad range of applications within bacteriology and might present new vistas as a point-of-care instrument in clinical and veterinary settings.
authors.affiliation: Department of Animal Science, Faculty of Science and Technology, Aarhus University, Tjele, Denmark. marlene.fredborg@agrsci.dk
authors.forename: Marlene
authors.initials: M
authors.lastname: Fredborg
authors.forename: Klaus R
authors.initials: KR
authors.lastname: Andersen
authors.forename: Erik
authors.initials: E
authors.lastname: Jørgensen
authors.forename: Aida
authors.initials: A
authors.lastname: Droce
authors.forename: Tom
authors.initials: T
authors.lastname: Olesen
authors.forename: Bent B
authors.initials: BB
authors.lastname: Jensen
authors.forename: Flemming S
authors.initials: FS
authors.lastname: Rosenvinge
authors.forename: Teis E
authors.initials: TE
authors.lastname: Sondergaard
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D057205:Automation, Laboratory / Q000379:methods; D001419:Bacteria / Q000187:drug effects* / Q000254:growth & development* / Q000302:isolation & purification; D055499:Catheter-Related Infections / Q000382:microbiology; D004195:Disease Models, Animal; D008826:Microbial Sensitivity Tests / Q000379:methods*; D061848:Optical Imaging / Q000379:methods*; D013552:Swine; D013997:Time Factors; D059008:Time-Lapse Imaging / Q000379:methods*; D014552:Urinary Tract Infections / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents