title: Transfected early growth response gene-1 DNA enzyme prevents stenosis and occlusion of autogenous vein graft in vivo.
abstract: The aim of this study was to detect the inhibitory action of the early growth response gene-1 DNA enzyme (EDRz) as a carrying agent by liposomes on vascular smooth muscle cell proliferation and intimal hyperplasia. An autogenous vein graft model was established. EDRz was transfected to the graft vein. The vein graft samples were obtained on each time point after surgery. The expression of the EDRz transfected in the vein graft was detected using a fluorescent microscope. Early growth response gene-1 (Egr-1) mRNA was measured using reverse transcription-PCR and in situ hybridization. And the protein expression of Egr-1 was detected by using western blot and immunohistochemistry analyses. EDRz was located at the media of the vein graft from 2 to 24 h, 7 h after grafting. The Egr-1 protein was mainly located in the medial VSMCs, monocytes, and endothelium cells during the early phase of the vein graft. The degree of VSMC proliferation and thickness of intima were obviously relieved compared with the no-gene therapy group. EDRz can reduce Egr-1 expression in autogenous vein grafts, effectively restrain VSMC proliferation and intimal hyperplasia, and prevent vascular stenosis and occlusion after vein graft.
authors.affiliation: First Department of General Surgery, The First Affiliated Hospital of Jiamusi University, Jiamusi 154002, China.
authors.forename: Chengwei
authors.initials: C
authors.lastname: Liu
authors.forename: Xuesong
authors.initials: X
authors.lastname: Zhang
authors.forename: Shi
authors.initials: S
authors.lastname: Wang
authors.forename: Mingxun
authors.initials: M
authors.lastname: Cheng
authors.forename: Chuanyu
authors.initials: C
authors.lastname: Liu
authors.forename: Shuqing
authors.initials: S
authors.lastname: Wang
authors.forename: Xinhua
authors.initials: X
authors.lastname: Hu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D003251:Constriction, Pathologic / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D051766:Early Growth Response Protein 1 / Q000008:administration & dosage / Q000235:genetics / Q000378:metabolism*; D006083:Graft Occlusion, Vascular; D006801:Humans; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D008856:Microscopy, Fluorescence; D032389:Myocytes, Smooth Muscle / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D014182:Transplantation, Autologous; D017539:Tunica Intima / Q000378:metabolism / Q000473:pathology; D014680:Veins / Q000378:metabolism* / Q000637:transplantation
chemical_list: D051766:Early Growth Response Protein 1; D008081:Liposomes