title: Complete genome sequence of acute viral necrosis virus associated with massive mortality outbreaks in the Chinese scallop, Chlamys farreri.
abstract: BACKGROUND
  Acute viral necrosis virus (AVNV) is the causative agent of a serious disease resulting in high mortality in cultured Chinese scallops, Chlamys farreri. We have sequenced and analyzed the complete genome of AVNV.
  
  
  RESULTS
  The AVNV genome is a linear, double-stranded DNA molecule of 210,993 bp with a nucleotide composition of 38.5% G + C. A total of 123 open reading frames were predicted to encode functional proteins, ranging from 41 to 1,878 amino acid residues. The DNA sequence of AVNV is 97% identical to that of ostreid herpesvirus 1 (OsHV-1), and the amino acid sequences of the encoded proteins of these two viruses are 94-100% identical. The genomic organization of AVNV is similar to that of OsHV-1, and consists of two unique regions (170.4 kb and 3.4 kb, respectively), each flanked by two inverted repeats (7.6 kb and 10.2 kb, respectively), with a third unique region (1.5 kb) situated between the two internal repeats.
  
  
  CONCLUSIONS
  Our results indicate that AVNV is a variant of OsHV-1. The AVNV genome sequence provides information useful for understanding the evolution and divergence of OsHV-1 in marine molluscs.
authors.affiliation: Maricultural Organism Disease Control and Pathogenic Molecular Biology Laboratory, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Qingdao, 266071, China.
authors.forename: Weicheng
authors.initials: W
authors.lastname: Ren
authors.forename: Haixia
authors.initials: H
authors.lastname: Chen
authors.forename: Tristan
authors.initials: T
authors.lastname: Renault
authors.forename: Yuyong
authors.initials: Y
authors.lastname: Cai
authors.forename: Changming
authors.initials: C
authors.lastname: Bai
authors.forename: Chongming
authors.initials: C
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Huang
mesh_terms: D000818:Animals; D001482:Base Composition; D002681:China; D004247:DNA / Q000737:chemistry / Q000235:genetics; D004267:DNA Viruses / Q000235:genetics* / Q000302:isolation & purification; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics*; D023061:Gene Order; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D049897:Pectinidae / Q000821:virology*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D026801:Synteny
chemical_list: D004279:DNA, Viral; D004247:DNA