title: Artemether combined with shRNA interference of vascular cell adhesion molecule-1 significantly inhibited the malignant biological behavior of human glioma cells.
abstract: Artemether is the derivative extracted from Chinese traditional herb and originally used for malaria. Artemether also has potential therapeutic effects against tumors. Vascular cell adhesion molecule-1 (VCAM-1) is an important cell surface adhesion molecule associated with malignancy of gliomas. In this work, we investigated the role and mechanism of artemether combined with shRNA interference of VCAM-1 (shRNA-VCAM-1) on the migration, invasion and apoptosis of glioma cells. U87 human glioma cells were treated with artemether at various concentrations and shRNA interfering technology was employed to silence the expression of VCAM-1. Cell viability, migration, invasiveness and apoptosis were assessed with MTT, wound healing, Transwell and Annexin V-FITC/PI staining. The expression of matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9) and phosphorylated Akt (p-Akt) was checked by Western blot assay. Results showed that artemether and shRNA-VCAM-1 not only significantly inhibited the migration, invasiveness and expression of MMP-2/9 and p-Akt, but also promoted the apoptosis of U87 cells. Combined treatment of both displayed the maximum inhibitory effects on the malignant biological behavior of glioma cells. Our work revealed the potential therapeutic effects of artemether and antiVCAM-1 in the treatments of gliomas.
authors.affiliation: Department of Neurosurgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning Province, People's Republic of China.
authors.forename: Ying-Bin
authors.initials: YB
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Hu
authors.forename: Zhen
authors.initials: Z
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Yi-Xue
authors.initials: YX
authors.lastname: Xue
authors.forename: Yi-Long
authors.initials: YL
authors.lastname: Yao
authors.forename: Bo
authors.initials: B
authors.lastname: Yu
authors.forename: Yun-Hui
authors.initials: YH
authors.lastname: Liu
mesh_terms: D017304:Annexin A5; D017209:Apoptosis / Q000187:drug effects; D000077549:Artemether; D037621:Artemisinins / Q000494:pharmacology*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D016650:Fluorescein-5-isothiocyanate; D005910:Glioma / Q000188:drug therapy*; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009361:Neoplasm Invasiveness / Q000517:prevention & control; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D013778:Tetrazolium Salts; D013844:Thiazoles; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism*
chemical_list: D017304:Annexin A5; D037621:Artemisinins; D004365:Drugs, Chinese Herbal; D034741:RNA, Small Interfering; D013778:Tetrazolium Salts; D013844:Thiazoles; D019010:Vascular Cell Adhesion Molecule-1; D000077549:Artemether; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; C022616:thiazolyl blue; D016650:Fluorescein-5-isothiocyanate