title: Reduction of intestinal electrogenic glucose absorption after duodenojejunal bypass in a mouse model.
abstract: BACKGROUND
  The role of intestinal glucose absorption in reducing glycemic levels after duodenojejunal bypass (DJB) is unclear. We hypothesized that DJB surgery decreases intestinal electrogenic glucose absorption by sodium glucose co-transporter 1 (SGLT1), leading to decreased glucose absorption and reduced blood glucose. Six groups of C57/BL6 mice were studied (n = 6 each) 2 weeks, 1 month, and 2 months after DJB or sham surgery.
  
  
  METHODS
  Daily weight and food intake were measured for 1 month and an oral glucose tolerance test was performed 2 months after surgery. Electrogenic glucose absorption was quantified in an Ussing chamber according to the sodium-dependent increase of short-circuit current (Isc). Intestinal morphology was assessed by hematoxylin and eosin staining. Expression of SGLT1 was determined by quantitative PCR and Western blotting.
  
  
  RESULTS
  The DJB mice lost weight compared with the sham group and exhibited significant adaptive changes, with increased villus height, crypt depth, and villus surface area. Oral glucose absorption was significantly reduced in the DJB mice compared with the sham group. Glucose-induced Isc was significantly lower in the DJB mice than in the sham mice 1 and 2 months after surgery, indicating that SGLT1 activity was reduced after DJB. Transcript levels and protein abundance of SGLT1 after DJB were also decreased compared with the sham group.
  
  
  CONCLUSIONS
  DJB surgery reduced intestinal glucose absorption by reducing the activity and expression of the glucose transporter SGLT1, which represents a potential therapeutic target for patients with diabetes.
authors.affiliation: Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, First Affiliated Hospital, School of Medicine, Key Laboratory of Combined Multi-Organ Transplantation, Ministry of Public Health, Key Laboratory of Organ Transplantation, Zhejiang University, Hangzhou, Zhejiang Province 310003, China. shengyan@zju.edu.cn
authors.forename: Sheng
authors.initials: S
authors.lastname: Yan
authors.forename: Fei
authors.initials: F
authors.lastname: Sun
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Xiang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Ding
authors.forename: Zhongjie
authors.initials: Z
authors.lastname: Lu
authors.forename: Yang
authors.initials: Y
authors.lastname: Tian
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.forename: Jinhua
authors.initials: J
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Penghong
authors.initials: P
authors.lastname: Song
authors.forename: Lin
authors.initials: L
authors.lastname: Zhou
authors.forename: Shusen
authors.initials: S
authors.lastname: Zheng
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000601:surgery; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism* / Q000601:surgery; D004195:Disease Models, Animal; D004386:Duodenum / Q000378:metabolism / Q000601:surgery*; D015390:Gastric Bypass; D005951:Glucose Tolerance Test; D007150:Immunohistochemistry; D007408:Intestinal Absorption; D007421:Intestine, Small / Q000378:metabolism* / Q000601:surgery; D007583:Jejunum / Q000378:metabolism / Q000601:surgery*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D051269:Sodium-Glucose Transporter 1 / Q000378:metabolism
chemical_list: D001786:Blood Glucose; C495675:Slc5a1 protein, mouse; D051269:Sodium-Glucose Transporter 1