title: Novel antitumor agent, trilacunary Keggin-type tungstobismuthate, inhibits proliferation and induces apoptosis in human gastric cancer SGC-7901 cells.
abstract: A new one-dimensional chain-like compound of tungstobismuthate, [(W(OH)2)2 (Mn(H2O)3)2(Na3(H2O)14)(BiW9O33)2](Himi)2·16H2O (1) (imi = iminazole), has been synthesized in aqueous solution. The structure of 1 was identified by elemental analysis, IR, thermogravimetry (TG), X-ray photoelectron spectroscopy (XPS), (183)W-NMR, and single crystal X-ray diffraction. To investigate the inhibitory effect of 1 on human gastric adenocarcinoma SGC-7901 cells, cell proliferation and apoptosis initiation were examined by MTT assay (MTT = 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazoliumbromide), flow cytometry, nuclear staining, transmission electron microscopy, single cell gel electrophoresis, DNA fragmentation, and Western blotting. The results showed that 1 inhibited cell proliferation and induced apoptosis in SGC-7901 cells in dose-dependent manner. In addition, 1 also decreased the expression of bcl-2 protein and nuclear factor-κB p65 protein in SGC-7901 cells. And expression of bcl-2 protein exhibits a decreasing trend with increase of concentration of 1. Thus, 1 possessed a potential antitumor activity in SGC-7901 cells. This suggests that polyoxotungstates will provide a promising and novel antitumor agent in prevention and treatment of gastric adenocarcinoma.
authors.affiliation: Key Laboratory of Synthesis of Functional Materials and Green Catalysis Colleges of Heilongjiang Province, Department of Chemical Engineering, Harbin Normal University, Harbin, Heilongjiang 150025, People's Republic of China.
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.forename: Bai-Bin
authors.initials: BB
authors.lastname: Zhou
authors.forename: Kai
authors.initials: K
authors.lastname: Yu
authors.forename: Zhan-Hua
authors.initials: ZH
authors.lastname: Su
authors.forename: Song
authors.initials: S
authors.lastname: Gao
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Chu
authors.forename: Jia-Ren
authors.initials: JR
authors.lastname: Liu
authors.forename: Guo-Yu
authors.initials: GY
authors.lastname: Yang
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy* / Q000473:pathology; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D006801:Humans; D008958:Models, Molecular; D016328:NF-kappa B / Q000032:analysis; D056951:Photoelectron Spectroscopy; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000032:analysis; D013270:Stomach / Q000187:drug effects* / Q000473:pathology; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000473:pathology; D017973:Tungsten Compounds / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D016328:NF-kappa B; D019253:Proto-Oncogene Proteins c-bcl-2; D017973:Tungsten Compounds