title: Tagging the rolling circle products with nanocrystal clusters for cascade signal increase in the detection of miRNA.
abstract: A new method to label and detect the long ssDNA products from rolling circle (RC) amplification was reported. ZnS nanocrystal clusters (NCCs) were used to tag the RC products. The NCCs release millions of cations to trigger millions of metal-responsive dyes for cascade signal amplification. Selective and sensitive quantification of miRNA was demonstrated; and the NCCs delivered much lower detection limits compared to using peroxidase or quantum dots as the labels.
authors.affiliation: Department of Chemistry, University of California, Riverside, CA 92521, USA.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Yao
authors.forename: Kenneth
authors.initials: K
authors.lastname: Flack
authors.forename: Liangzi
authors.initials: L
authors.lastname: Ding
authors.forename: Wenwan
authors.initials: W
authors.lastname: Zhong
mesh_terms: D019921:Biotinylation; D004261:DNA Replication; D004277:DNA, Single-Stranded / Q000032:analysis* / Q000096:biosynthesis* / Q000235:genetics; D057230:Limit of Detection; D059346:Magnets / Q000737:chemistry; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics; D053758:Nanoparticles / Q000737:chemistry*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D013194:Staining and Labeling
chemical_list: D004277:DNA, Single-Stranded; D035683:MicroRNAs