title: Biochemical characters and antibiotic susceptibility of Staphylococcus aureus isolates.
abstract: OBJECTIVE
  To observe the biochemical characters and antibiotic susceptibility of isolated Staphylococcus aureus (S. auerus) strains against some conventional and traditional antibiotics.
  
  
  METHODS
  Thirty post operative pathogenic isolated S. aureus strains were used in this study. Bacterial culture was done in Mueller-Hinton broth at 37 °C. Characters of these strains were determined by traditional biochemical tests such as hydrolysis test of gelatin, urea, galactose, starch and protein, and fermentation of lactose and sucrose. Antibiotic susceptibility were carried out by minimum inhibitory concentration test, minium bactericidal concentration test, disc agar diffusion test and brain heart infusion oxacillin screening agar.
  
  
  RESULTS
  From this study, it was observed that 100% S. aureus isolates showed positive results in gelatin, urea and galactose hydrolysis test, 50% isolates were positive in starch hydrolysis test, 35% in protein hydrolysis test, 100% isolates in lactose fermenting test, but no isolate was positive in sucrose fermenting test. Antibiotic susceptibility testing suggested that 20% of isolates were resistant to kanamycin and 46.67% were resistant to oxacillin.
  
  
  CONCLUSIONS
  These findings show that all these isolates have gelatin, urea, galactose hydrolysis and lactose fermenting activity. 20% of these isolates were resistant to kanamycin and 46.67% were resistant to oxacillin.
authors.affiliation: Immunology and Microbiology Laboratory, Department of Human Physiology with Community Health, Vidyasagar University, Midnapore-721 102, West Bengal, India.
authors.forename: Subhankari Prasad
authors.initials: SP
authors.lastname: Chakraborty
authors.forename: Santanu Kar
authors.initials: SK
authors.lastname: Mahapatra
authors.forename: Somenath
authors.initials: S
authors.lastname: Roy
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D052978:Disk Diffusion Antimicrobial Tests; D005690:Galactose / Q000378:metabolism; D005780:Gelatin / Q000378:metabolism; D006868:Hydrolysis; D008826:Microbial Sensitivity Tests; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000302:isolation & purification / Q000378:metabolism*; D013213:Starch / Q000378:metabolism; D014508:Urea / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D014508:Urea; D005780:Gelatin; D013213:Starch; D005690:Galactose
keywords: Antibiotic susceptibility; Bacterial culture; Biochemical character; Disc agar diffusion; Minimum inhibitory concentration; Oxacillin; Pathogen; Staphylococcus aureus