title: Effect of nitrate availability on expression of multi sensor histidine kinase gene in Spirulina platensis PCC 7345.
abstract: Addition of nitrate (NO3 (-)) to a suspension of NO3 (-)-depleted Spirulina platensis PCC 7345 restored back the PSII reaction centre efficiency. Using RAPD/RT-PCR differential display, we have detected the upregulation of a novel gene in filaments starved of nitrogen when enriched with NO3 (-). Semiquantitative RT-PCR clearly shows that NO3 (-) repletion induces the expression of this gene. Its nucleotide sequence is homologous to multi sensor histidine kinase (hstk) from Spirulina platensis NIES-39 and Spirulina maxima CS-328 genes. The open reading frame of this gene encodes a protein of 98 amino acids contains sensory domain of PAS domain.
authors.affiliation: Centre for biotechnology, Biological Sciences Group, Birla Institute of Technology and Science, Pilani, 333031 Rajasthan India.
authors.forename: Pradeep Kumar
authors.initials: PK
authors.lastname: Logeswaran
authors.forename: Swapnil
authors.initials: S
authors.lastname: Srivastav
authors.forename: Krithika
authors.initials: K
authors.lastname: Karunakaran
authors.forename: Vinky
authors.initials: V
authors.lastname: Kohli
authors.forename: Priya
authors.initials: P
authors.lastname: Munjal
authors.forename: Bhagavatula
authors.initials: B
authors.lastname: Vani
keywords: Differential display; Histidine kinase; PSII reaction centre efficiency; Spirulina