title: Simple yet stringent screening methodologies for evaluation of putative transformants for abiotic stress tolerance: salt and cadmium stress as a paradigm.
abstract: Rigorous and stringent screening methodologies to select transformants at both seedling and plant level under cadmium or NaCl stress were developed. At seedling level, two screening strategies were standardized. One involved germination on filter paper/agar in the presence of either CdCl2 (125 μM) or NaCl (350-450 mM) for 9 days and selection of tolerant putative transformants. The other involved germination of the seedlings on soilrite by irrigation of 450 mM NaCl. Further, at plant level, in vitro evaluation for stress tolerance involved a simple leaf senescence bioassay. Combination of the seedling and plant level screening strategies would result in the initial identification of promising transformants for further analysis.
authors.affiliation: Department of Crop Physiology, University of Agricultural Sciences, GKVK Campus, Bangalore, 560 065 India.
authors.forename: Sushma M
authors.initials: SM
authors.lastname: Awaji
authors.forename: V
authors.initials: V
authors.lastname: Nagaveni
authors.lastname: Prashantkumar
authors.forename: S
authors.initials: S
authors.lastname: Hanjagi
authors.forename: D N
authors.initials: DN
authors.lastname: Madhvi
authors.forename: V R
authors.initials: VR
authors.lastname: Sashidhar
authors.forename: Rohini
authors.initials: R
authors.lastname: Sreevathsa
keywords: Abiotic stress; evaluation; methodology; transgenics