title: Protein partners of KCTD proteins provide insights about their functional roles in cell differentiation and vertebrate development.
abstract: The KCTD family includes tetramerization (T1) domain containing proteins with diverse biological effects. We identified a novel member of the KCTD family, BTBD10. A comprehensive analysis of protein-protein interactions (PPIs) allowed us to put forth a number of testable hypotheses concerning the biological functions for individual KCTD proteins. In particular, we predict that KCTD20 participates in the AKT-mTOR-p70 S6k signaling cascade, KCTD5 plays a role in cytokinesis in a NEK6 and ch-TOG-dependent manner, KCTD10 regulates the RhoA/RhoB pathway. Developmental regulator KCTD15 represses AP-2α and contributes to energy homeostasis by suppressing early adipogenesis. TNFAIP1-like KCTD proteins may participate in post-replication DNA repair through PCNA ubiquitination. KCTD12 may suppress the proliferation of gastrointestinal cells through interference with GABAb signaling. KCTD9 deserves experimental attention as the only eukaryotic protein with a DNA-like pentapeptide repeat domain. The value of manual curation of PPIs and analysis of existing high-throughput data should not be underestimated.
authors.affiliation: Research Center for Medical Genetics RAMS, Moscow, Russian Federation, Russia.
authors.forename: Mikhail
authors.initials: M
authors.lastname: Skoblov
authors.forename: Andrey
authors.initials: A
authors.lastname: Marakhonov
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Marakasova
authors.forename: Anna
authors.initials: A
authors.lastname: Guskova
authors.forename: Vikas
authors.initials: V
authors.lastname: Chandhoke
authors.forename: Aybike
authors.initials: A
authors.lastname: Birerdinc
authors.forename: Ancha
authors.initials: A
authors.lastname: Baranova
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D004261:DNA Replication; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D000072379:NIMA-Related Kinases; D009687:Nuclear Proteins / Q000235:genetics / Q000502:physiology*; D015221:Potassium Channels / Q000235:genetics / Q000502:physiology; D024642:Potassium Channels, Voltage-Gated / Q000235:genetics / Q000502:physiology; D018809:Proliferating Cell Nuclear Antigen / Q000235:genetics / Q000502:physiology; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000502:physiology; D012097:Repressor Proteins / Q000235:genetics / Q000502:physiology*; D038762:Ribosomal Protein S6 Kinases, 70-kDa / Q000235:genetics / Q000502:physiology; D015398:Signal Transduction; D058570:TOR Serine-Threonine Kinases / Q000235:genetics / Q000502:physiology; D054875:Ubiquitination; D014714:Vertebrates / Q000235:genetics*
chemical_list: C494432:BTBD10 protein, human; D047908:Intracellular Signaling Peptides and Proteins; C539187:KCTD10 protein, human; C552182:KCTD15 protein, human; C521187:KCTD5 protein, human; C580589:KCTD9 protein, human; D009687:Nuclear Proteins; D015221:Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D018809:Proliferating Cell Nuclear Antigen; D012097:Repressor Proteins; C546842:MTOR protein, human; C459456:NEK6 protein, human; D000072379:NIMA-Related Kinases; D017346:Protein Serine-Threonine Kinases; D038762:Ribosomal Protein S6 Kinases, 70-kDa; D058570:TOR Serine-Threonine Kinases