title: Characterization of pyrene biodegradation by white-rot fungus Polyporus sp. S133.
abstract: A white-rot fungus of Polyporus sp. S133 was isolated from an oil-polluted soil. The metabolism of pyrene by this fungus was investigated in liquid medium with 5 mg of the compound. Depletion of pyrene was evident during the 30-day growth period and was 21% and 90%, respectively, in cometabolism and metabolism of pyrene alone. Pyrene was absorbed to fungal cells or biodegraded to form simpler structural compounds. Seventy-one percent of eliminated pyrene was transformed by Polyporus sp. S133 into other compounds, whereas only 18% was absorbed in the fungal cell. The effects of pH and temperature on biomass production of Polyporus sp. S133 for pyrene were examined; the properties of laccase and 1,2-dioxygenase produced by Polyporus sp. S133 during pyrene degradation were investigated. The optimal values of pH were 3, 5, and 4 for laccase, 1,2-dioxygenase, and biomass production, respectively, whereas the optimal values of temperature were 25 °C for laccase and 50 °C for 1,2-dioxygenase and biomass production. Under optimal conditions, pyrene was mainly metabolized to 1-hydroxypyrene and gentisic acid. The structure of 1-hydroxypyrene and gentisic acid was determined by gas chromatography-mass spectrometry after identification using thin-layer chromatography.
authors.affiliation: Faculty of Civil Engineering, Institute of Environmental and Water Resources Management, Universiti Teknologi Malaysia, Johor Bahru, Malaysia. hadibarata@utm.my
authors.forename: Tony
authors.initials: T
authors.lastname: Hadibarata
authors.forename: Risky Ayu
authors.initials: RA
authors.lastname: Kristanti
authors.forename: Mohamad Ali
authors.initials: MA
authors.lastname: Fulazzaky
authors.forename: Agung Endro
authors.initials: AE
authors.lastname: Nugroho
mesh_terms: D001673:Biodegradation, Environmental; D018533:Biomass; D001711:Biotransformation; D049308:Dioxygenases / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000378:metabolism; D010578:Petroleum; D055492:Polyporus / Q000302:isolation & purification / Q000378:metabolism*; D011721:Pyrenes / Q000302:isolation & purification* / Q000378:metabolism*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000302:isolation & purification* / Q000378:metabolism*; D013696:Temperature
chemical_list: D010578:Petroleum; D011721:Pyrenes; D012989:Soil Pollutants; C030984:pyrene; D042845:Laccase; D049308:Dioxygenases