title: Effect of human mesenchymal stem cells on the growth of HepG2 and Hela cells.
abstract: Human mesenchymal stem cells (hMSCs) accumulate at carcinomas and have a great impact on cancer cell's behavior. Here we demonstrated that hMSCs could display both the promotional and inhibitive effects on growth of HepG2 and Hela cells by using the conditioned media, indirect co-culture, and cell-to-cell co-culture. Cell growth was increased following the addition of lower proportion of hMSCs while decreased by treatment of higher proportion of hMSCs. We also established a novel noninvasive label way by using internalizing quantum dots (i-QDs) for study of cell-cell contact in the co-culture, which was effective and sensitive for both tracking and distinguishing different cells population without the disturbance of cells. Furthermore, we investigated the role of hMSCs in regulation of cell growth and showed that mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K) signaling pathways were involved in hMSC-mediated cell inhibition and proliferation. Our findings suggested that hMSCs regulated cancer cell function by providing a suitable environment, and the discovery from the study would provide some clues for development of effective strategy for hMSC-based cancer therapies.
authors.affiliation: National Institute of Advanced Industrial Science and Technology, Ibaraki, Japan.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Long
authors.forename: Rena
authors.initials: R
authors.lastname: Matsumoto
authors.forename: Pengyuan
authors.initials: P
authors.lastname: Yang
authors.forename: Toshimasa
authors.initials: T
authors.lastname: Uemura
mesh_terms: D002450:Cell Communication / Q000187:drug effects / Q000502:physiology*; D049109:Cell Proliferation / Q000187:drug effects; D018920:Coculture Techniques; D017077:Culture Media, Conditioned / Q000494:pharmacology; D005260:Female; D006367:HeLa Cells / Q000187:drug effects / Q000473:pathology* / Q000502:physiology; D056945:Hep G2 Cells / Q000187:drug effects / Q000473:pathology* / Q000502:physiology; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology / Q000503:physiopathology; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D020929:Mitogen-Activated Protein Kinase Kinases / Q000502:physiology; D019869:Phosphatidylinositol 3-Kinases / Q000502:physiology; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D002583:Uterine Cervical Neoplasms / Q000473:pathology / Q000503:physiopathology
chemical_list: D017077:Culture Media, Conditioned; D019869:Phosphatidylinositol 3-Kinases; D020929:Mitogen-Activated Protein Kinase Kinases