title: Protection of IFNAR (-/-) mice against bluetongue virus serotype 8, by heterologous (DNA/rMVA) and homologous (rMVA/rMVA) vaccination, expressing outer-capsid protein VP2.
abstract: The protective efficacy of recombinant vaccines expressing serotype 8 bluetongue virus (BTV-8) capsid proteins was tested in a mouse model. The recombinant vaccines comprised plasmid DNA or Modified Vaccinia Ankara viruses encoding BTV VP2, VP5 or VP7 proteins. These constructs were administered alone or in combination using either a homologous prime boost vaccination regime (rMVA/rMVA) or a heterologous vaccination regime (DNA/rMVA). The DNA/rMVA or rMVA/rMVA prime-boost were administered at a three week interval and all of the animals that received VP2 generated neutralising antibodies. The vaccinated and non-vaccinated-control mice were subsequently challenged with a lethal dose of BTV-8. Mice vaccinated with VP7 alone were not protected. However, mice vaccinated with DNA/rMVA or rMVA/rMVA expressing VP2, VP5 and VP7 or VP2 alone were all protected.
authors.affiliation: The Pirbright Institute, Pirbright, Woking, Surrey, United Kingdom.
authors.forename: Tamara Kusay
authors.initials: TK
authors.lastname: Jabbar
authors.forename: Eva
authors.initials: E
authors.lastname: Calvo-Pinilla
authors.forename: Francisco
authors.initials: F
authors.lastname: Mateos
authors.forename: Simon
authors.initials: S
authors.lastname: Gubbins
authors.forename: Abdelghani
authors.initials: A
authors.lastname: Bin-Tarif
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Bachanek-Bankowska
authors.forename: Oya
authors.initials: O
authors.lastname: Alpar
authors.forename: Javier
authors.initials: J
authors.lastname: Ortego
authors.forename: Haru-Hisa
authors.initials: HH
authors.lastname: Takamatsu
authors.forename: Peter Paul Clement
authors.initials: PP
authors.lastname: Mertens
authors.forename: Javier
authors.initials: J
authors.lastname: Castillo-Olivares
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D001819:Bluetongue / Q000097:blood / Q000276:immunology* / Q000517:prevention & control* / Q000821:virology; D001820:Bluetongue virus / Q000276:immunology*; D036022:Capsid Proteins / Q000276:immunology*; D002645:Chickens; D002522:Chlorocebus aethiops; D051379:Mice; D010957:Plasmids / Q000276:immunology; D012367:RNA, Viral / Q000097:blood; D053633:Receptor, Interferon alpha-beta / Q000172:deficiency* / Q000378:metabolism; D014611:Vaccination; D019444:Vaccines, DNA / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology*; D014709:Vero Cells; D014766:Viremia / Q000276:immunology / Q000517:prevention & control / Q000821:virology
chemical_list: D057134:Antibodies, Neutralizing; D036022:Capsid Proteins; D012367:RNA, Viral; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic; D053633:Receptor, Interferon alpha-beta