title: Noninvasive photodetachment of stem cells on tunable conductive polymer nano thin films: selective harvesting and preserved differentiation capacity.
abstract: Viable mesenchymal stem cells (MSCs) were efficiently and selectively harvested by near-infrared (NIR) light using the photothermal effect of a conductive polymer nano thin film. The poly(3,4-ethylenedioxy thiophene) (PEDOT)-coated cell culture surfaces were prepared via a simple and fast solution-casting polymerization (SCP) technique. The absorption of PEDOT thin films in the NIR region was effectively triggered cell harvesting upon exposure to an NIR source. By controlling the NIR absorption of the PEDOT film through electrochemical doping or growing PEDOT with different thin film thickness from 70 to 300 nm, the proliferation and harvesting of MSCs on the PEDOT surface were controlled quantitatively. This light-induced cell detachment method based on PEDOT films provides the temporal and spatial control of cell harvesting, as well as cell patterning. The harvested stem cells were found to be alive and well proliferated despite the use of temperature increase by NIR. More importantly, the harvested MSCs by this method preserved their intrinsic characteristics as well as multilineage differentiation capacities. This PEDOT surfaces could be used for repetitive culture and detachment of MSCs or for efficient selection or depletion of a specific subset from heterogeneous population during culture of various tissue-derived cells because there were no photodegradation and photobreakage in the PEDOT films by NIR exposure.
authors.affiliation: Department of Chemical and Biomolecular Engineering, Cell Therapy Center, Severance Hospital, Yonsei University College of Medicine, and Seodaemun-gu, Seoul 120-749, Korea.
authors.forename: Jungmok
authors.initials: J
authors.lastname: You
authors.forename: June Seok
authors.initials: JS
authors.lastname: Heo
authors.forename: Jeonghun
authors.initials: J
authors.lastname: Kim
authors.forename: Teahoon
authors.initials: T
authors.lastname: Park
authors.forename: Byeonggwan
authors.initials: B
authors.lastname: Kim
authors.forename: Han-Soo
authors.initials: HS
authors.lastname: Kim
authors.forename: Youjeong
authors.initials: Y
authors.lastname: Choi
authors.forename: Hyun Ok
authors.initials: HO
authors.lastname: Kim
authors.forename: Eunkyoung
authors.initials: E
authors.lastname: Kim
mesh_terms: D000042:Absorption; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry* / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects / Q000528:radiation effects; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects* / Q000528:radiation effects; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D004563:Electrochemistry; D006801:Humans; D007259:Infrared Rays; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000528:radiation effects; D049329:Nanostructures / Q000737:chemistry*; D058105:Polymerization; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology*; D013499:Surface Properties
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D011108:Polymers; C121383:poly(3,4-ethylene dioxythiophene)