title: Characterization and analysis of human arterial tissue secretome by 2-DE and nLC-MS/MS.
abstract: Early detection of cardiovascular diseases and knowledge of underlying mechanisms is essential. Tissue secretome studies resemble more closely to the in vivo situation, showing a much narrower protein concentrations dynamic range than plasma. In the present chapter, we detail the characterization and analysis of human arterial tissue secretome by two-dimensional electrophoresis (2-DE) and nano-liquid chromatography on-line coupled to mass spectrometry (nLC-MS/MS). General strategies shown here can be extended to other tissue secretome studies.
authors.affiliation: Department of Vascular Physiopathology, Hospital Nacional de Parapléjicos, SESCAM, Toledo, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: de la Cuesta
authors.forename: Maria G
authors.initials: MG
authors.lastname: Barderas
authors.forename: Enrique
authors.initials: E
authors.lastname: Calvo
authors.forename: Irene
authors.initials: I
authors.lastname: Zubiri
authors.forename: Aroa S
authors.initials: AS
authors.lastname: Maroto
authors.forename: Juan Antonio
authors.initials: JA
authors.lastname: Lopez
authors.forename: Fernando
authors.initials: F
authors.lastname: Vivanco
authors.forename: Gloria
authors.initials: G
authors.lastname: Alvarez-Llamas
mesh_terms: D001158:Arteries / Q000166:cytology* / Q000378:metabolism*; D001940:Breast / Q000098:blood supply; D002853:Chromatography, Liquid; D003331:Coronary Vessels / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D036103:Nanotechnology; D010446:Peptide Fragments / Q000302:isolation & purification / Q000378:metabolism; D059748:Proteolysis; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D010446:Peptide Fragments