title: BACE1 levels correlate with phospho-tau levels in human cerebrospinal fluid.
abstract: Previous studies have investigated the activity and protein levels of BACE1, the β-secretase, in the brain and cerebrospinal fluid (CSF) of Alzheimer's disease (AD) patients, however, results remain contradictory. We present here a highly specific and sensitive BACE1 ELISA, which allows measuring accurately BACE1 levels in human samples. We find that BACE1 levels in CSF of AD patients and other neurological disorder (OND) patients are slightly increased when compared to those of a non-neurological disorder control group (NND). BACE1 levels in CSF were well correlated with total-tau and hyperphosphorylated tau levels in the CSF, suggesting that the recorded alterations in BACE1 levels correlate with cell death and neurodegeneration.
authors.affiliation: VIB Center for the Biology of Disease and Center for Human Genetics, KULeuven, Herestraat 49, 3000 Leuven, Belgium.
authors.forename: Soraia
authors.initials: S
authors.lastname: Barao
authors.forename: Lujia
authors.initials: L
authors.lastname: Zhou
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Adamczuk
authors.forename: Tine
authors.initials: T
authors.lastname: Vanhoutvin
authors.forename: Fred
authors.initials: F
authors.lastname: van Leuven
authors.forename: David
authors.initials: D
authors.lastname: Demedts
authors.forename: Anne-Catherine
authors.initials: AC
authors.lastname: Vijverman
authors.forename: Xavier
authors.initials: X
authors.lastname: Bossuyt
authors.forename: Rik
authors.initials: R
authors.lastname: Vandenberghe
authors.forename: Bart
authors.initials: B
authors.lastname: De Strooper
mesh_terms: D000368:Aged; D000544:Alzheimer Disease / Q000134:cerebrospinal fluid* / Q000175:diagnosis*; D053829:Amyloid Precursor Protein Secretases / Q000096:biosynthesis / Q000134:cerebrospinal fluid* / Q000737:chemistry; D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000096:biosynthesis / Q000134:cerebrospinal fluid* / Q000737:chemistry; D015415:Biomarkers / Q000134:cerebrospinal fluid; D005260:Female; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009422:Nervous System Diseases / Q000134:cerebrospinal fluid / Q000175:diagnosis; D010766:Phosphorylation / Q000502:physiology; D017433:Protein Structure, Secondary; D016037:Single-Blind Method; D016875:tau Proteins / Q000134:cerebrospinal fluid* / Q000737:chemistry
chemical_list: D015415:Biomarkers; D016875:tau Proteins; D053829:Amyloid Precursor Protein Secretases; D016282:Aspartic Acid Endopeptidases; C509280:BACE1 protein, human