title: [Study on association between ERCC2/XPD single nucleotide polymorphisms and DNA adducts damage induced by B[a]P in vitro].
abstract: OBJECTIVE
  To evaluate the association between DNA damage repair capacity induced by environment carcinogen benzo[a] pyrene (B [a] P) and ERCC2/XPD single nucleotide polymorphisms(SNP).
  
  
  METHODS
  8 ml peripheral bloods of 282 healthy ethnic Han people from Liao-ning province were collected, isolated lymphocytes and extracted DNA. The genotypes of ERCC2/XPD Lys751 Gln (rs13181), Asp312Asn (rs1799793), Arg156Arg (rs238406) were detected by Taqman real time PCR; BPDE-DNA adduct in vitro induced by B[a]P and S9 mixture in lymphocyte were detected by high performance liquid chromatography (HPLC).
  
  
  RESULTS
  The BPDE-DNA adduct levels of ERCC2/XPD Arg156Arg AA genotype were significantly higher than CC genotype. Compared with < or = 30 years, people at age of 50 - 70 years and > or = 70 years have higher BPDE-DNA adduct level (P < 0.05). Multiple covariates analysis showed SNP of ERCC2/XPD Arg156Arg (rs238406) and age have been related to BPDE-DNA adduct levels closely among all covariates (P < 0.05).
  
  
  CONCLUSIONS
  ERCC2/XPD Arg156Arg rs238406 polymorphisms may be associated with DNA repair capacity in excising BPDE-DNA adduct and A allele may increase the risks of cancer susceptibility.
authors.affiliation: China Medical University, Public Health Institute, Toxicology Department, Shenyang 110001, China. cfliuyh@126.com
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Liu
authors.forename: Tao
authors.initials: T
authors.lastname: Yu
authors.forename: Sha
authors.initials: S
authors.lastname: Xiao
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Guan
authors.forename: Dandan
authors.initials: D
authors.lastname: Li
authors.forename: Cuihong
authors.initials: C
authors.lastname: Jin
authors.forename: Qiufang
authors.initials: Q
authors.lastname: Liu
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yang
authors.forename: Shengwen
authors.initials: S
authors.lastname: Wu
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Lu
mesh_terms: D000328:Adult; D000368:Aged; D001564:Benzo(a)pyrene / Q000633:toxicity*; D018736:DNA Adducts / Q000378:metabolism*; D004249:DNA Damage / Q000187:drug effects; D004260:DNA Repair / Q000187:drug effects*; D004785:Environmental Pollutants / Q000633:toxicity; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D051759:Xeroderma Pigmentosum Group D Protein / Q000235:genetics*
chemical_list: D018736:DNA Adducts; D004785:Environmental Pollutants; D001564:Benzo(a)pyrene; D051759:Xeroderma Pigmentosum Group D Protein; C497292:ERCC2 protein, human