title: Aurora B kinase is required for cytokinesis through effecting spindle structure.
abstract: The spindle, composed of microtubules and associated proteins, serves as major machinery for cell division. Aurora B is a chromosome passenger protein that has important functions in mitosis. Its dynamic distribution at mitosis goes along with spindle structure and dynamics. We used the siRNA technique to knockdown protein expression and immunofluorescence technique to follow Aurora B during mitosis. Aurora B regulates microtubule plus ends as mitosis progresses, including both kinetochore and polar microtubules. Interactions between Aurora B and polar microtubule plus ends lead to failure in cytokinesis and abnormal midbody structure. We think Aurora B may not only play a role as kinase, but regulate microtubule plus ends.
authors.affiliation: Department of Hepatobiliary Surgery, Tianjin Medical University Cancer Institute and Hospital, Key Laboratory of Cancer Prevention and Therapy, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wu
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Tianhao
authors.initials: T
authors.lastname: Mu
authors.forename: Tianqiang
authors.initials: T
authors.lastname: Song
authors.forename: Dengwen
authors.initials: D
authors.lastname: Li
mesh_terms: D064107:Aurora Kinase B / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D048749:Cytokinesis; D006801:Humans; D061986:MCF-7 Cells; D008870:Microtubules / Q000737:chemistry / Q000378:metabolism; D008938:Mitosis; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D008941:Spindle Apparatus / Q000378:metabolism*
chemical_list: D034741:RNA, Small Interfering; D064107:Aurora Kinase B