title: Novel fluorescent probe for highly sensitive bioassay using sequential enzyme-linked immunosorbent assay-capillary isoelectric focusing (ELISA-cIEF).
abstract: This paper presents a novel rhodamine diphosphate molecule that allows highly sensitive detection of proteins by employing sequential enzyme-linked immunosorbent assay and capillary isoelectric focusing (ELISA-cIEF). Seven-fold improvement in the immunoassay sensitivity and a 1-2 order of magnitude lower detection limit has been demonstrated by taking advantage of the combination of the enzyme-based signal amplification of ELISA and the concentration of enzyme reaction products by cIEF.
authors.affiliation: Graduate School of Engineering, Osaka Prefecture University, Sakai City, Osaka 599-8531, Japan.
authors.forename: Terence G
authors.initials: TG
authors.lastname: Henares
authors.forename: Yuta
authors.initials: Y
authors.lastname: Uenoyama
authors.forename: Yuto
authors.initials: Y
authors.lastname: Nogawa
authors.forename: Ken
authors.initials: K
authors.lastname: Ikegami
authors.forename: Daniel
authors.initials: D
authors.lastname: Citterio
authors.forename: Koji
authors.initials: K
authors.lastname: Suzuki
authors.forename: Shun-ichi
authors.initials: S
authors.lastname: Funano
authors.forename: Kenji
authors.initials: K
authors.lastname: Sueyoshi
authors.forename: Tatsuro
authors.initials: T
authors.lastname: Endo
authors.forename: Hideaki
authors.initials: H
authors.lastname: Hisamoto
mesh_terms: D000178:Acrylamides / Q000737:chemistry; D001681:Biological Assay / Q000379:methods*; D002197:Capillary Action; D060166:Capillary Tubing; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D007525:Isoelectric Focusing / Q000379:methods*; D057230:Limit of Detection; D011506:Proteins / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D012235:Rhodamines / Q000737:chemistry
chemical_list: D000178:Acrylamides; D005456:Fluorescent Dyes; D011506:Proteins; D012235:Rhodamines; C429790:poly(N,N-dimethylacrylamide); C049025:rhodamine 110