title: Suppressor of K+ transport growth defect 1 (SKD1) interacts with RING-type ubiquitin ligase and sucrose non-fermenting 1-related protein kinase (SnRK1) in the halophyte ice plant.
abstract: SKD1 (suppressor of K+ transport growth defect 1) is an AAA-type ATPase that functions as a molecular motor. It was previously shown that SKD1 accumulates in epidermal bladder cells of the halophyte Mesembryanthemum crystallinum. SKD1 knock-down Arabidopsis mutants showed an imbalanced Na+/K+ ratio under salt stress. Two enzymes involved in protein post-translational modifications that physically interacted with McSKD1 were identified. McCPN1 (copine 1), a RING-type ubiquitin ligase, has an N-terminal myristoylation site that links to the plasma membrane, a central copine domain that interacts with McSKD1, and a C-terminal RING domain that catalyses protein ubiquitination. In vitro ubiquitination assay demonstrated that McCPN1 was capable of mediating ubiquitination of McSKD1. McSnRK1 (sucrose non-fermenting 1-related protein kinase) is a Ser/Thr protein kinase that contains an N-terminal STKc catalytic domain to phosphorylate McSKD1, and C-terminal UBA and KA1 domains to interact with McSKD1. The transcript and protein levels of McSnRK1 increased as NaCl concentrations increased. The formation of an SKD1-SnRK1-CPN1 ternary complex was demonstrated by yeast three-hybrid and bimolecular fluorescence complementation. It was found that McSKD1 preferentially interacts with McSnRK1 in the cytosol, and salt induced the re-distribution of McSKD1 and McSnRK1 towards the plasma membrane via the microtubule cytoskeleton and subsequently interacted with RING-type E3 McCPN1. The potential effects of ubiquitination and phosphorylation on McSKD1, such as changes in the ATPase activity and cellular localization, and how they relate to the functions of SKD1 in the maintenance of Na+/K+ homeostasis under salt stress, are discussed.
authors.affiliation: Department of Life Sciences, National Chung Hsing University, Taichung 40227, Taiwan.
authors.forename: Chih-Pin
authors.initials: CP
authors.lastname: Chiang
authors.forename: Chang-Hua
authors.initials: CH
authors.lastname: Li
authors.forename: Yingtzy
authors.initials: Y
authors.lastname: Jou
authors.forename: Yu-Chan
authors.initials: YC
authors.lastname: Chen
authors.forename: Ya-Chung
authors.initials: YC
authors.lastname: Lin
authors.forename: Fang-Yu
authors.initials: FY
authors.lastname: Yang
authors.forename: Nu-Chuan
authors.initials: NC
authors.lastname: Huang
authors.forename: Hungchen Emilie
authors.initials: HE
authors.lastname: Yen
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism / Q000502:physiology*; D035723:Mesembryanthemum / Q000201:enzymology / Q000378:metabolism / Q000502:physiology*; D010766:Phosphorylation; D010940:Plant Proteins / Q000378:metabolism / Q000502:physiology*; D015221:Potassium Channels / Q000378:metabolism / Q000502:physiology*; D011499:Protein Processing, Post-Translational / Q000502:physiology; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism / Q000502:physiology*; D055051:Salt-Tolerant Plants / Q000201:enzymology / Q000378:metabolism / Q000502:physiology*; D020798:Two-Hybrid System Techniques; D054875:Ubiquitination / Q000502:physiology
chemical_list: D010940:Plant Proteins; D015221:Potassium Channels; D017346:Protein Serine-Threonine Kinases; D000251:Adenosine Triphosphatases
keywords: AAA-type ATPase; RING-type ubiquitin ligase; SKD1; SnRK1.; protein kinase; protein ubiquitination; salt stress