title: Phytochemical screening and in vitro bioactivities of the extracts of aerial part of Boerhavia diffusa Linn.
abstract: OBJECTIVE
  To investigate the bioactivities of crude n-hexane, ethyl acetate and methanol extracts of aerial part of Boerhavia diffusa Linn. (B. diffusa) and its phytochemical analysis.
  
  
  METHODS
  The identification of phytoconstituents and assay of antioxidant, thrombolytic, cytotoxic, antimicrobial activities were conducted using specific standard in vitro procedures.
  
  
  RESULTS
  The results showed that the plant extracts were a rich source of phytoconstituents. Methanol extract showed higher antioxidant, thrombolytic activity and less cytotoxic activity than those of n-hexane and ethyl acetate extracts of B. diffusa. Among the bioactivities, antioxidant activity was the most notable compared to the positive control and thus could be a potential rich source of natural antioxidant. In case of antimicrobial screening, crude extracts of the plant showed remarkable antibacterial activity against tested microorganisms. All the extracts showed significant inhibitory activity against Candida albicuns, at a concentration of 1000 µg/disc.
  
  
  CONCLUSIONS
  The present findings suggest that, the plant widely available in Bangladesh, could be a prominent source of medicinally important natural compounds.
authors.affiliation: Department of Pharmacy, East West University, Dhaka-1212, Bangladesh.
authors.forename: Apurba Sarker
authors.initials: AS
authors.lastname: Apu
authors.forename: Mahmuda Sultana
authors.initials: MS
authors.lastname: Liza
authors.forename: A T M
authors.initials: AT
authors.lastname: Jamaluddin
authors.forename: Md Amran
authors.initials: MA
authors.lastname: Howlader
authors.forename: Repon Kumer
authors.initials: RK
authors.lastname: Saha
authors.forename: Farhana
authors.initials: F
authors.lastname: Rizwan
authors.forename: Nishat
authors.initials: N
authors.lastname: Nasrin
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry; D001156:Artemia / Q000187:drug effects; D001419:Bacteria / Q000187:drug effects; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors; D007928:Lethal Dose 50; D008826:Microbial Sensitivity Tests; D009569:Nitric Oxide / Q000037:antagonists & inhibitors; D039281:Nyctaginaceae / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry; D064209:Phytochemicals / Q000737:chemistry*; D010851:Picrates / Q000037:antagonists & inhibitors; D035261:Plant Components, Aerial / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology* / Q000633:toxicity
chemical_list: D000890:Anti-Infective Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D010636:Phenols; D064209:Phytochemicals; D010851:Picrates; D010936:Plant Extracts; D009569:Nitric Oxide; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Antimicrobial; Antioxidant; Boerhavia diffusa; Cytotoxic; DPPH; NO; Thrombolytic