title: The conjugates of gold nanorods and chlorin e6 for enhancing the fluorescence detection and photodynamic therapy of cancers.
abstract: Gold nanorods (AuNRs) were conjugated with chlorin e6 (Ce6), a commonly used photosensitizer, to form AuNRs-Ce6 by electrostatic binding. Due to the strong surface plasmon resonance coupling, the fluorescence of conjugated Ce6 was enhanced 3-fold and the production of singlet oxygen was increased 1.4-fold. AuNRs-Ce6 were taken up by the HeLa and KB cell lines more easily than free Ce6, enhancing the intracellular delivery of Ce6. The increased cellular amount of Ce6 leads to a 3-fold more efficient photodynamic killing of these two cell lines. This demonstrates the potential of this approach to improve photodynamic detection and therapy of cancers.
authors.affiliation: State Key Laboratory of Surface Physics and Department of Physics and Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Fudan University, 220, Handan Road, Shanghai 200433, China. jychen@fudan.edu.cn.
authors.forename: Xiao
authors.initials: X
authors.lastname: Huang
authors.forename: Xue-Jiao
authors.initials: XJ
authors.lastname: Tian
authors.forename: Wu-Li
authors.initials: WL
authors.lastname: Yang
authors.forename: Benjamin
authors.initials: B
authors.lastname: Ehrenberg
authors.forename: Ji-Yao
authors.initials: JY
authors.lastname: Chen
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D002735:Chlorophyllides; D006046:Gold / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D018613:Microscopy, Confocal; D043942:Nanotubes / Q000737:chemistry*; D009369:Neoplasms / Q000188:drug therapy; D010778:Photochemotherapy; D017319:Photosensitizing Agents / Q000138:chemical synthesis* / Q000627:therapeutic use / Q000633:toxicity; D011166:Porphyrins / Q000737:chemistry*; D026082:Singlet Oxygen / Q000737:chemistry / Q000378:metabolism; D055672:Static Electricity
chemical_list: D002735:Chlorophyllides; D017319:Photosensitizing Agents; D011166:Porphyrins; D026082:Singlet Oxygen; C062985:phytochlorin; D006046:Gold