title: Association between gene polymorphisms of IRAK-M and the susceptibility of sepsis.
abstract: The aim of this study was to explore the association between the single-nucleotide polymorphisms of interleukin-1 receptor-associated kinase-M (IRAK-M) gene and the susceptibility of sepsis. The allele frequency and genotype distribution of IRAK-M gene polymorphisms were assessed in 118 controls and 82 sepsis patients by semiquantitative polymerase chain reaction and restriction fragment length polymorphism (RFLP) analysis. The plasma levels of tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6) were detected by enzyme-linked immunosorbent assay. Associations between IRAK-M polymorphisms and the susceptibility of sepsis were analyzed by Cox regression. Data were analyzed by the χ(2) test and the Student's t test, whenever appropriate. Statistical calculations were performed by using statistical package SPSS version 18.0. The genotype distribution of IRAK-M+22148 polymorphism significantly differed between the sepsis and control groups (P < 0.0001). The frequency of the G allele was remarkably more common in the sepsis group than that of the control group (P < 0.0001). However, the frequency of the A allele was significantly less common in the sepsis group than that of control group (P < 0.0001). Moreover, the plasma levels (in picograms per milliliter) of TNF-α and IL-6 in patients with G/G genotype were greatly higher than those with A/A genotype after lipopolysaccharide stimulation (P < 0.05). The genetic polymorphism of IRAK-M+22148 G>A is associated with the susceptibility of sepsis. The G/G genotype of IRAK-M increases the risk of developing sepsis, and the A/A genotype may play a protective role in the process of developing sepsis.
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Jinan University, Guangzhou, 510630, China.
authors.forename: Gao-Hong
authors.initials: GH
authors.lastname: Dong
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Gong
authors.forename: Jin-Zheng
authors.initials: JZ
authors.lastname: Li
authors.forename: Yu-Hong
authors.initials: YH
authors.lastname: Luo
authors.forename: Zhen-Dong
authors.initials: ZD
authors.lastname: Li
authors.forename: Pei-Zhi
authors.initials: PZ
authors.lastname: Li
authors.forename: Kun
authors.initials: K
authors.lastname: He
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D018836:Inflammation Mediators / Q000097:blood; D053592:Interleukin-1 Receptor-Associated Kinases / Q000235:genetics*; D015850:Interleukin-6 / Q000097:blood*; D008070:Lipopolysaccharides; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D018805:Sepsis / Q000235:genetics* / Q000382:microbiology; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*
chemical_list: C508600:IL6 protein, human; D018836:Inflammation Mediators; D015850:Interleukin-6; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; C507771:IRAK3 protein, human; D053592:Interleukin-1 Receptor-Associated Kinases