title: Improved detection rate of cytogenetic abnormalities in chronic lymphocytic leukemia and other mature B-cell neoplasms with use of CpG-oligonucleotide DSP30 and interleukin 2 stimulation.
abstract: Detection of cytogenetic abnormalities requires successful culture of the clonal population to obtain metaphase chromosomes for study, and as such, has been hampered by low mitotic indices of mature B cells in culture. Our study presents data on the improved abnormality detection rate with the use of a CpG-oligonucleotide/interleukin 2 (OL/IL-2) culture protocol for mature B-cell neoplasms, including chronic lymphocytic leukemia (CLL) and non-CLL specimens. The increased detection rate of abnormalities, compared with unstimulated culture and traditional pokeweed mitogen culture, was statistically significant for both CLL and non-CLL neoplasms. For CLL specimens, our data also showed that for cytogenetically visible aberrations, OL/IL-2 was as, if not more, sensitive than detection with interphase fluorescence in situ hybridization (iFISH). Use of OL/IL-2 allowed a number of abnormalities to be detected, which were not covered by specific iFISH panels, especially balanced translocations. Therefore, OL/IL-2 stimulation improves diagnostic sensitivity and increases discovery rate of novel prognostic findings.
authors.affiliation: Department of Pathology, UMASS Memorial Medical Center, Worcester, MA 01605, USA.
authors.forename: Min
authors.initials: M
authors.lastname: Shi
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: Cipollini
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Crowley-Bish
authors.forename: Anne W
authors.initials: AW
authors.lastname: Higgins
authors.forename: Hongbo
authors.initials: H
authors.lastname: Yu
authors.forename: Patricia M
authors.initials: PM
authors.lastname: Miron
mesh_terms: D001854:Bone Marrow Cells / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects; D002869:Chromosome Aberrations; D018899:CpG Islands / Q000187:drug effects / Q000235:genetics; D003582:Cytogenetics / Q000379:methods*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007376:Interleukin-2 / Q000494:pharmacology*; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000175:diagnosis* / Q000235:genetics; D008677:Metaphase; D009841:Oligonucleotides; D014407:Tumor Cells, Cultured
chemical_list: C508594:IL2 protein, human; D007376:Interleukin-2; D009841:Oligonucleotides