title: p53 Attenuates the oncogenic Ras-induced epithelial-mesenchymal transition in human mammary epithelial cells.
abstract: Inactivation of the tumor suppressor p53 and activation of the oncogene Ras are the two most pivotal events in tumor development. However, potential intersection between p53 and Ras activity during an EMT process, which plays a crucial role during malignant tumor progression, remains elusive. Here, we report that increased expression of wild type p53 suppressed H-Ras(V12)-induced EMT phenotypes and restrained stem cell properties, through downregulation of MEK-ERK signaling pathways. In vivo experiments showed that p53 was able to inhibit H-Ras(V12)-induced tumor growth of human mammary epithelial cells. This study elucidates a novel correlation between the tumor suppressor gene p53 and the oncogene Ras in regulating EMT program, and expands the knowledge about the function of p53 in EMT process.
authors.affiliation: The Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, PR China.
authors.forename: Jianchao
authors.initials: J
authors.lastname: Zhang
authors.forename: Yang
authors.initials: Y
authors.lastname: Lei
authors.forename: Xiaoge
authors.initials: X
authors.lastname: Gao
authors.forename: Qian
authors.initials: Q
authors.lastname: Liang
authors.forename: Lili
authors.initials: L
authors.lastname: Li
authors.forename: Jingxin
authors.initials: J
authors.lastname: Feng
authors.forename: Pingfu
authors.initials: P
authors.lastname: Hou
authors.forename: Liping
authors.initials: L
authors.lastname: Han
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Baiqu
authors.initials: B
authors.lastname: Huang
authors.forename: Jun
authors.initials: J
authors.lastname: Lu
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D058750:Epithelial-Mesenchymal Transition; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D020868:Gene Silencing; D011905:Genes, ras; D006801:Humans; D020935:MAP Kinase Signaling System; D042361:Mammary Glands, Human / Q000166:cytology*; D051379:Mice; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000502:physiology*
chemical_list: D017931:DNA Primers; D016159:Tumor Suppressor Protein p53