title: Proteomic analysis of epithelioma papulosum cyprini cells infected with spring viremia of carp virus.
abstract: Spring viremia of carp (SVC), caused by spring viremia of carp virus (SVCV) is an important disease due to its drastic effects on carp fisheries in many countries. To better understand molecular responses to SVCV infection, two dimensional electrophoresis (2-DE) and MALDI-TOF/TOF were performed to investigate altered proteins in epithelioma papulosum cyprini cells (EPCs). Differentially expressed proteins in mock-infected EPCs and SVCV-infected EPCs were compared. A total of 54 differentially expressed spots were successfully identified (33 up-regulated spots and 21 down-regulated spots) which include cytoskeleton proteins, macromolecular biosynthesis-associated proteins, stress response proteins, signal transduction proteins, energy metabolism, and ubiquitin proteasome pathway-associated proteins. Moreover, 7 corresponding genes of the differentially expressed proteins were quantified using real time RT-PCR to examine their transcriptional profiles. The presence of four selected cellular proteins (beta-actin, gamma1-actin, heat shock cognate 71 kDa protein and annexin A2) associated with the spring viremia of carp virus (SVCV) particles was validated by Western blot assay. This study provides dynamic and useful protein-related information to further understand the underlying pathogenesis of SVCV infection.
authors.affiliation: Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors.forename: Liyue
authors.initials: L
authors.lastname: Liu
authors.forename: Qin
authors.initials: Q
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Lin
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Yuanan
authors.initials: Y
authors.lastname: Lu
authors.forename: Weimin
authors.initials: W
authors.lastname: Wang
authors.forename: Junfa
authors.initials: J
authors.lastname: Yuan
authors.forename: Lijuan
authors.initials: L
authors.lastname: Li
authors.forename: Xueqin
authors.initials: X
authors.lastname: Liu
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D002347:Carps; D005393:Fish Diseases / Q000235:genetics / Q000378:metabolism / Q000821:virology*; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D020543:Proteome; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D018353:Rhabdoviridae Infections / Q000235:genetics / Q000378:metabolism / Q000662:veterinary* / Q000821:virology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000662:veterinary; D053719:Tandem Mass Spectrometry / Q000662:veterinary; D013997:Time Factors; D014407:Tumor Cells, Cultured; D018116:Vesiculovirus / Q000502:physiology
chemical_list: D029941:Fish Proteins; D020543:Proteome