title: Pyrrolizidine alkaloids from Liparis nervosa with inhibitory activities against LPS-induced NO production in RAW264.7 macrophages.
abstract: Six pyrrolizidine alkaloids were isolated from the whole herb of Liparis nervosa together with two previously known ones. Their structures were elucidated by extensive spectroscopic analyses and chemical reactions. The cytotoxicity of the isolates was evaluated against A549, HepG2, and MCF-7 human cancer cell lines; however, no significant growth inhibition was observed. All compounds were evaluated for the inhibition of LPS-induced nitric oxide (NO) production in RAW264.7 macrophages, and most significantly inhibited NO production with IC50 values in the range of 2.16-38.25 μM.
authors.affiliation: School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, Sichuan, PR China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Huang
authors.forename: Xian-li
authors.initials: XL
authors.lastname: Zhou
authors.forename: Cui-juan
authors.initials: CJ
authors.lastname: Wang
authors.forename: You-song
authors.initials: YS
authors.lastname: Wang
authors.forename: Feng
authors.initials: F
authors.lastname: Xiao
authors.forename: Lian-hai
authors.initials: LH
authors.lastname: Shan
authors.forename: Zhi-yun
authors.initials: ZY
authors.lastname: Guo
authors.forename: Jie
authors.initials: J
authors.lastname: Weng
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D056945:Hep G2 Cells; D006801:Humans; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors* / Q000494:pharmacology; D061986:MCF-7 Cells; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008968:Molecular Conformation; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D029595:Orchidaceae / Q000737:chemistry*; D011763:Pyrrolizidine Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D014407:Tumor Cells, Cultured
chemical_list: D008070:Lipopolysaccharides; D011763:Pyrrolizidine Alkaloids; D009569:Nitric Oxide
keywords: Liparis nervosa; NO production inhibitor; Nervogenic acid derivatives; Nervosine; Orchidaceae; Pyrrolizidine alkaloids