title: Effect of bacteriophage lysin on lysogens.
abstract: OBJECTIVE
  To study the effect of phage lysin on the growth of lysogens.
  
  
  METHODS
  Sputum specimens processed by modified Petroff's method were respectively treated with phagebiotics in combination with lysin and lysin alone. The specimens were incubated at 37 °C for 4 days. At the end of day 1, 2, 3 and day 4, the specimens were streaked on blood agar plates and incubated at 37 °C for 18-24 hours. The growth of normal flora observed after day 1 was considered as lysogens.
  
  
  RESULTS
  Sputum specimens treated with phagebiotics-lysin showed the growth of lysogens. When specimens treated with lysin alone, lysogen formation was avoided and normal flora was controlled.
  
  
  CONCLUSIONS
  Lysin may have no effect on the growth of lysogens.
authors.affiliation: Tuberculosis Research Centre, Chennai, India.
authors.forename: Balaji
authors.initials: B
authors.lastname: Subramanyam
authors.forename: Vanaja
authors.initials: V
authors.lastname: Kumar
mesh_terms: D001419:Bacteria / Q000187:drug effects* / Q000254:growth & development; D001435:Bacteriophages / Q000201:enzymology*; D008242:Lysogeny; D050296:Microbial Viability / Q000187:drug effects*; D009088:Mucoproteins / Q000378:metabolism*; D013183:Sputum / Q000382:microbiology; D013696:Temperature; D013997:Time Factors
chemical_list: D009088:Mucoproteins; C010393:lysin, gastropoda
keywords: Bacteriophages; Normal flora; Phage lysin