title: Production of ginsenoside Rd from ginsenoside Rc by α-L-arabinofuranosidase from Caldicellulosiruptor saccharolyticus.
abstract: Ginsenoside Rd was produced from ginsenoside Rc using a thermostable recombinant alpha-L-arabinofuranosidase from Caldicellulosiruptor saccharolyticus. The optimal reaction conditions for the production of ginsenoside Rd from Rc were pH 5.5, 80 degrees C, 227 U enzyme/ml, and 8.0 g/l ginsenoside Rc in the presence of 30% (v/v) n-hexane. Under these conditions, the enzyme produced 7.0 g/l ginsenoside Rd after 30 min, with a molar yield of 100% and a productivity of 14 g l(-1) h(-1). The conversion yield and productivity of ginsenoside Rd are the highest reported thus far among enzymatic transformations.
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, Korea.
authors.forename: Kyung-Chul
authors.initials: KC
authors.lastname: Shin
authors.forename: Gi-Woong
authors.initials: GW
authors.lastname: Lee
authors.forename: Deok-Kun
authors.initials: DK
authors.lastname: Oh
mesh_terms: D001709:Biotechnology / Q000379:methods*; D001711:Biotransformation; D036145:Ginsenosides / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006094:Gram-Positive Bacteria / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013696:Temperature
chemical_list: D036145:Ginsenosides; D011994:Recombinant Proteins; C044462:ginsenoside Rc; D006026:Glycoside Hydrolases; C019713:alpha-N-arabinofuranosidase; C049863:ginsenoside Rd