title: Effect of newly synthesized 1,2,4-triazino[5,6-b]indole-3-thione derivatives on olfactory bulbectomy induced depression in rats.
abstract: OBJECTIVE
  To study the derivatives of 1,2,4-triazino[5,6-b]indole-3-thione for antidepressant activity in olfactory bulbectomized (OBX) rats. Out of various derivatives tested for acute tail suspension test, the two derivatives showing prominent action were selected for bilateral olfactory bulbectomy model of chronic depression in rats.
  
  
  METHODS
  The sub acute effects of 14-day oral pretreatment of two derivatives labeled as 3a (70 mg/kg) and 3r (70 mg/kg), imipramine (20 mg/kg), fluoxetine (30 mg/kg) and moclobemide (15 mg/kg) were evaluated on bilateral bulbectomy induced rise in body weight, hyperphagia, hyperactivity, and on sexual dysfunction. The serum sodium concentration, body temperature, and heart rate were also recorded.
  
  
  RESULTS
  The derivatives 3a and 3r showed reversal of drop in body weight, reversed OBX induced hyperactivity, normalized body temperature, heart rate, and serum sodium concentration. In elevated maze test, moclobemide, 3a, 3r treatment significantly reduced time spent in open arm as compared to OBX rats. 3a and 3r also improved sexual behavior parameters.
  
  
  CONCLUSIONS
  The present study shows promising antidepressant action and provides a proof of concept for the chronic treatment of 3a, 3r to treat depression.
authors.affiliation: Department of Pharmacology, STES's Sinhgad Institute of Pharmacy, Narhe, Pune-411041,India.
authors.forename: Urmila M
authors.initials: UM
authors.lastname: Aswar
authors.forename: Padmaja P
authors.initials: PP
authors.lastname: Kalshetti
authors.forename: Suhas M
authors.initials: SM
authors.lastname: Shelke
authors.forename: Sharad H
authors.initials: SH
authors.lastname: Bhosale
authors.forename: Subhash L
authors.initials: SL
authors.lastname: Bodhankar
mesh_terms: D000081:Acetamides / Q000494:pharmacology*; D000083:Acetanilides / Q000494:pharmacology*; D000818:Animals; D000928:Antidepressive Agents / Q000494:pharmacology*; D001522:Behavior, Animal / Q000187:drug effects; D003863:Depression / Q000188:drug therapy* / Q000209:etiology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D005473:Fluoxetine / Q000494:pharmacology*; D007099:Imipramine / Q000494:pharmacology*; D008297:Male; D020912:Moclobemide / Q000494:pharmacology*; D000857:Olfaction Disorders / Q000150:complications / Q000473:pathology*; D009830:Olfactory Bulb / Q000601:surgery*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014227:Triazines / Q000494:pharmacology*
chemical_list: C000597604:2-(5H-(1,2,4)triazino(5,6-b)indol-3-ylthio)-N-cyclohexylacetamide; C000597603:2-(5H-(1,2,4)triazino(5,6-b)indol-3-ylthio)-N-phenylacetamide; D000081:Acetamides; D000083:Acetanilides; D000928:Antidepressive Agents; D014227:Triazines; D005473:Fluoxetine; D007099:Imipramine; D020912:Moclobemide
keywords: 1,2,4 -triazino-[5,6-b]indole-3-thione; Antidepressant activity; Olfactory bulbectomy