title: The effect of polymorphism in gene of insulin-like growth factor-I on the serum periparturient concentration in Holstein dairy cows.
abstract: OBJECTIVE
  To investigate the relationship between polymorphism within the 5'-untranslated region (5'-UTR) of IGF-I gene and its periparturient concentration in Iranian Holstein dairy cows.
  
  
  METHODS
  Blood samples (5 mL, n = 37) were collected by caudal venipuncture from each animal into sample tubes containing the EDTA and DNA was extracted from blood. In order to measure IGF-I concentration the collection of blood samples (n = 111) was also done at 14 d before calving (prepartum), 25 and 45 d postpartum.
  
  
  RESULTS
  We found evidence for a significant effect of C to T mutation in position 512 of IGF-I gene on its serum concentration in dairy cows in Iran. Cows with CC genotype had significantly higher concentration (Mean±SD) of IGF-I at 14 d prepartum (91.8±18.1) µg/L compared to those with TT genotype (73.3±14.4) µg/L (P=0.04). A significant trend (quadratic) was found for IGF-I concentration, as higher in CC cows compared to ones with TT genotype, during the 14 d before calving to 45 d postpartum (P=0.01).
  
  
  CONCLUSIONS
  We concluded that C/T transition in the promoter region of IGF-I gene can influence the serum concentration of IGF-I in periparturient dairy cows.
authors.affiliation: Department of Clinical Science, School of Veterinary Medicine, Shiraz University, Shiraz 71345, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Mirzaei
authors.forename: H
authors.initials: H
authors.lastname: Sharifiyazdi
authors.forename: M R
authors.initials: MR
authors.lastname: Ahmadi
authors.forename: T
authors.initials: T
authors.lastname: Ararooti
authors.forename: A Rowshan
authors.initials: AR
authors.lastname: Ghasrodashti
authors.forename: A
authors.initials: A
authors.lastname: Kadivar
mesh_terms: D000483:Alleles; D000818:Animals; D002417:Cattle; D005260:Female; D056726:Genetic Association Studies; D005838:Genotype; D007334:Insulin-Like Growth Factor I / Q000235:genetics* / Q000378:metabolism*; D009154:Mutation; D058725:Peripartum Period; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide
chemical_list: D007334:Insulin-Like Growth Factor I
keywords: Dairy cows; Insulin-like growth factor-I (IGF-I); Periparturient; Polymorphism; Serum concentration