title: The genetic study utility of a hexaploid wheat DH population with non-recombinant A- and B-genomes.
abstract: To study the D-genome of the wild wheat relative Aegilops tauschii Cosson at the hexaploid level, we developed a synthetic doubled-haploid (DH) hexaploid wheat population, SynDH3. This population was derived from the spontaneous chromosome doubling of triploid F1 hybrid plants obtained from a cross between Triticum turgidum ssp. dicoccon PI377655 and A. tauschii ssp. strangulata AS66 × ssp. tauschii AS87. SynDH3 is a diploidization-hexaploid DH population containing recombinant D chromosomes from two different A. tauschii genotypes, with A and B chromosomes from T. turgidum being homogenous across the entire population. Using this population, we constructed a genetic map. Of the 440 markers used to construct the map, 421 (96%) were assigned to 12 linkage groups; these included 346 Diversity Arrays Technology (DArT) and 75 simple sequence repeat (SSR) markers. The total map length of the seven D chromosomes spanned 916.27 cM, with an average length of 130.90 cM per chromosome and an average distance between markers of 3.47 cM. Seven segregation distortion regions were detected on seven linkage groups. Out of 50 markers shared with those on a common wheat map, 37 showed a consistent order. The utility of the diploidization-hexaploid DH population for mapping qualitative trait genes was confirmed using the dominant glaucousness-inhibiting gene W2 (I) as an example.
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130 Sichuan P.R. China.
authors.forename: Ming
authors.initials: M
authors.lastname: Hao
authors.forename: Jixiang
authors.initials: J
authors.lastname: Chen
authors.forename: Lianquan
authors.initials: L
authors.lastname: Zhang
authors.forename: Jiangtao
authors.initials: J
authors.lastname: Luo
authors.forename: Zhongwei
authors.initials: Z
authors.lastname: Yuan
authors.forename: Zehong
authors.initials: Z
authors.lastname: Yan
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Wenjie
authors.initials: W
authors.lastname: Chen
authors.forename: Yuming
authors.initials: Y
authors.lastname: Wei
authors.forename: Huaigang
authors.initials: H
authors.lastname: Zhang
authors.forename: Youliang
authors.initials: Y
authors.lastname: Zheng
authors.forename: Dengcai
authors.initials: D
authors.lastname: Liu
keywords: Aegilops tauschii; Allopolyploid; Doubled-haploid; Segregation distortion