title: [Determination of a major constituent in deoxyspergualin drug by reversed-phase ion-pair chromatography].
abstract: A method was established for the determination of deoxyspergualin using reversed-phase ion-pair chromatography (RP-IPC). Several parameters, such as the chromatographic column, the types and concentration of ion-pair reagents, the concentration of the buffer and the pH value of the mobile phase were evaluated. The optimal separation conditions were as follows: C18 column (250 mm x 4.6 mm, 5 microm); mobile phase, 5 mmol/L di-potassium hydrogen phosphate (containing 5 mmol/L 1-pentanesulfonic acid sodium, pH 3.6 +/- 0.3)-acetonitrile (90: 10, v/v); flow rate, 1.0 mL/min; detection wavelength, 210 nm; column temperature, 30 degrees C; injection volume, 20 microL. Under the optimaized experimental conditions, good linear relationships was obtained and the limit of detection for deoxyspergualin was 0.5 mg/L.
authors.affiliation: North China Pharmaceutical Group New Drug Research and Development Co., Ltd, Shijiazhuang 050015, China.
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Cheng
authors.forename: Yali
authors.initials: Y
authors.lastname: Wang
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhang
authors.forename: Changqun
authors.initials: C
authors.lastname: Niu
mesh_terms: D056148:Chromatography, Reverse-Phase; D006146:Guanidines / Q000737:chemistry*; D007166:Immunosuppressive Agents / Q000737:chemistry
chemical_list: D006146:Guanidines; D007166:Immunosuppressive Agents; C037258:gusperimus