title: Comparison of influenza virus replication fidelity in vitro using selection pressure with monoclonal antibodies.
abstract: The replication fidelity of reassortant A/Fujian/411/02(H3N2)-like influenza viruses was assessed by in vitro detection of escape mutants developing under selective pressure from monoclonal antibodies. The results showed A/Wyoming/3/03(H3N2) possessed lower fidelity relative to the A/California/7/04(H3N2) and A/Wisconsin/57/05(H3N2) viruses through the emergence of mutant viruses carrying H156Q hemagglutinin mutation which allows antibody escape. Using the neutralization assay to compare the fidelity of reassortant pandemic A/California/7/09(H1N1) viruses, the pandemic virus was shown to possess relative higher fidelity compared to A/Wyoming/3/03. This higher fidelity may contribute to the lack of major antigenic changes in the pandemic virus since the emergence in 2009.
authors.affiliation: Faculty of Medicine, School of Medical Sciences, University of New South Wales, Sydney, New South Wales, Australia.
authors.forename: Karen Ka Yin
authors.initials: KK
authors.lastname: Wong
authors.forename: Steve
authors.initials: S
authors.lastname: Rockman
authors.forename: Chi
authors.initials: C
authors.lastname: Ong
authors.forename: Rowena
authors.initials: R
authors.lastname: Bull
authors.forename: Sacha
authors.initials: S
authors.lastname: Stelzer-Braid
authors.forename: William
authors.initials: W
authors.lastname: Rawlinson
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics*; D000914:Antibodies, Viral / Q000235:genetics*; D004285:Dogs; D005818:Genetic Engineering; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000276:immunology*; D006801:Humans; D057131:Immune Evasion; D053122:Influenza A Virus, H3N2 Subtype / Q000235:genetics / Q000276:immunology*; D061985:Madin Darby Canine Kidney Cells; D008958:Models, Molecular; D009154:Mutation; D009439:Neuraminidase / Q000235:genetics / Q000276:immunology; D009500:Neutralization Tests; D016865:Reassortant Viruses / Q000235:genetics / Q000276:immunology*; D014779:Virus Replication
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; C483468:hemagglutinin, human influenza A virus; D009439:Neuraminidase