title: Effect of morin, a flavonoid against DOCA-salt hypertensive rats: a dose dependent study.
abstract: OBJECTIVE
  To determine the protective effect of morin, a flavonoid against deoxycorticosterone acetate (DOCA)-salt induced hypertension in male Wistar rats.
  
  
  METHODS
  Hypertension was induced in uninephrectomized rats by weekly twice subcutaneous injection of DOCA (25 mg/kg bw) and 1% NaCl in the drinking water for six consecutive weeks. Effect of morin against DOCA-salt induced hypertension was evaluated by measuring blood pressure and performing biochemical estimations and histopathological examination of renal tissues.
  
  
  RESULTS
  DOCA-salt hypertensive rats showed considerably increased systolic and diastolic blood pressure, serum hepatic marker enzyme activities such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and gamma-glutamyl transpeptidase (GGT) and renal function markers (urea, uric acid and creatinine) in plasma. Oral administration of morin (25, 50 and 75 mg/kg bw) brought back all the above parameters to near normal level. Histopathology of kidney also confirmed the biochemical findings of this study. The effect at a dose of 50 mg/kg bw of morin was more pronounced than that of the other two doses (25 and 75 mg/kg bw).
  
  
  CONCLUSIONS
  These findings indicate that morin exhibits strong antihypertensive effect against DOCA-salt induced hypertension.
authors.affiliation: Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar-608 002, Tamil Nadu, India.
authors.forename: P
authors.initials: P
authors.lastname: Prahalathan
authors.forename: S
authors.initials: S
authors.lastname: Kumar
authors.forename: B
authors.initials: B
authors.lastname: Raja
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000959:Antihypertensive Agents / Q000008:administration & dosage*; D001794:Blood Pressure; D064791:Desoxycorticosterone Acetate / Q000633:toxicity*; D005419:Flavonoids / Q000008:administration & dosage*; D006973:Hypertension / Q000139:chemically induced* / Q000188:drug therapy*; D007668:Kidney / Q000473:pathology / Q000503:physiopathology; D007677:Kidney Function Tests; D008111:Liver Function Tests; D008297:Male; D017208:Rats, Wistar; D047309:Flavones
chemical_list: D000959:Antihypertensive Agents; D064791:Desoxycorticosterone Acetate; D005419:Flavonoids; C008548:morin; D047309:Flavones
keywords: Antihypertensive effect; Blood pressure; DOCA-salt; Hypertension; Morin; Oxidative stress; Uninephrectomy