title: Expression analysis of 13 ovine immune response candidate genes in Visna/Maedi disease progression.
abstract: Visna/Maedi virus (VMV) is a lentivirus that infects cells of the monocyte/macrophage lineage in sheep. Infection with VMV may lead to Visna/Maedi (VM) disease, which causes a multisystemic inflammatory disorder causing pneumonia, encephalitis, mastitis and arthritis. The role of ovine immune response genes in the development of VM disease is not fully understood. In this work, sheep of the Rasa Aragonesa breed were divided into two groups depending on the presence/absence of VM-characteristic clinical lesions in the aforementioned organs and the relative levels of candidate gene expression, including cytokines and innate immunity loci were measured by qPCR in the lung and udder. Sheep with lung lesions showed differential expression in five target genes: CCR5, TLR7, and TLR8 were up regulated and IL2 and TNFα down regulated. TNFα up regulation was detected in the udder.
authors.affiliation: Genetics, Physical Anthropology and Animal Physiology Department, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Bilbao, Spain.
authors.forename: Amaia
authors.initials: A
authors.lastname: Larruskain
authors.forename: Irantzu
authors.initials: I
authors.lastname: Bernales
authors.forename: Lluis
authors.initials: L
authors.lastname: Luján
authors.forename: Damián
authors.initials: D
authors.lastname: de Andrés
authors.forename: Beatriz
authors.initials: B
authors.lastname: Amorena
authors.forename: Begoña M
authors.initials: BM
authors.lastname: Jugo
mesh_terms: D000818:Animals; D005260:Female; D020869:Gene Expression Profiling / Q000662:veterinary; D015967:Gene Expression Regulation, Viral / Q000276:immunology*; D016014:Linear Models; D008168:Lung / Q000276:immunology / Q000821:virology*; D008321:Mammary Glands, Animal / Q000276:immunology / Q000821:virology*; D011021:Pneumonia, Progressive Interstitial, of Sheep / Q000276:immunology* / Q000821:virology; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012756:Sheep; D014790:Visna-maedi virus / Q000235:genetics / Q000276:immunology*
chemical_list: D012367:RNA, Viral