title: Isolation and partial characterization of cyclic lipopeptide antibiotics produced by Paenibacillus ehimensis B7.
abstract: BACKGROUND
  The prevalence of drug-resistant bacteria has encouraged the search for novel antimicrobial compounds. Food-associated microorganisms, as a source of new antibiotics, have recently received considerable attention. The objective of this study was to find novel antimicrobial agents produced by food microorganisms.
  
  
  RESULTS
  A bacterial strain B7, which has potent antimicrobial activity, was isolated from a sample of dairy waste. This strain was identified as Paenibacillus ehimensis based on the 16S rRNA gene sequence analysis, physiological and biochemical characterization. Two active compounds (PE1 and PE2) were obtained from P. ehimensis B7. Mass spectrometry (MS) analysis showed that the molecular masses of PE1 and PE2 were 1,114 and 1,100 Da, respectively. The tandem MS and amino acid analysis indicated that PE1 and PE2 were analogs of polypeptin, and PE2 was characterized as a new member of this family. Both compounds were active against all tested bacterial pathogens, including methicillin resistant Staphylococcus aureus, Escherichia coli, and pan-drug resistant Pseudomonas aeruginosa clinical isolate. Time-kill assays demonstrated that at 4 × MIC (minimum inhibitory concentration), PE1 and PE2 rapidly reduced the number of viable cells by at least 3-orders of magnitude, indicating that they were bactericidal antibiotics.
  
  
  CONCLUSIONS
  In the present work, two cationic lipopeptide antibiotics (PE1 and PE2) were isolated from P. ehimensis B7 and characterized. These two peptides showed broad antimicrobial activity against all tested human pathogens and are worthy of further study.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Huang
authors.forename: Yu
authors.initials: Y
authors.lastname: Hu
authors.forename: Linfei
authors.initials: L
authors.lastname: Shou
authors.forename: Mingxu
authors.initials: M
authors.lastname: Song
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial; D004926:Escherichia coli / Q000187:drug effects; D055666:Lipopeptides / Q000096:biosynthesis* / Q000737:chemistry / Q000494:pharmacology; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D056507:Paenibacillus / Q000737:chemistry* / Q000302:isolation & purification; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D053719:Tandem Mass Spectrometry
chemical_list: D000900:Anti-Bacterial Agents; D055666:Lipopeptides