title: Analysis of veterinary drug residues in cheese by ultra-high-performance LC coupled to triple quadrupole MS/MS.
abstract: A simple, reliable, and fast multiresidue method has been developed for the determination of 17 veterinary drugs belonging to several families (macrolides, sulfonamides, and anthelmintics) in cheese at trace levels. Ultra-high-performance LC coupled to MS/MS has been used for the analysis of these compounds in less than 9 min. Veterinary drug residues have been extracted from cheese samples using a QuEChERS (quick, easy, cheap, effective, rugged, and safe)-based extraction procedure without applying any further clean-up step. Matrix-matched calibration was used for quantification and recoveries were calculated at three concentration levels (10, 50, and 100 μg/kg). The obtained values ranged from 70 to 110% for the selected compounds except for tylosin and josamycin at 100 μg/kg (111.7 and 112.7%, respectively). Intra- and interday precision were also evaluated and RSDs were lower than 25% in all the cases. LOQs ranged from 0.3 μg/kg (for thiabendazole, oxfendazole, mebendazole, josamycin, and fenbendazole) to 10.5 μg/kg (abamectin), whereas decision limit and detection capability ranged from 2.3 (thiabendazole) to 11.3 (abamectin) and 4.2 (thiabendazole) to 14.3 μg/kg (abamectin), respectively. Finally, 13 samples were analyzed and traces of thiabendazole were detected in two different cheeses.
authors.affiliation: Department of Chemistry and Physics, Research Centre for Agricultural and Food Biotechnology (BITAL), University of Almería, Almería, Spain.
authors.forename: María Luz
authors.initials: ML
authors.lastname: Gómez Pérez
authors.forename: Roberto
authors.initials: R
authors.lastname: Romero-González
authors.forename: José Luis
authors.initials: JL
authors.lastname: Martínez Vidal
authors.forename: Antonia
authors.initials: A
authors.lastname: Garrido Frenich
mesh_terms: D000871:Anthelmintics / Q000032:analysis; D002611:Cheese / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000191:economics; D004350:Drug Residues / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry; D018942:Macrolides / Q000032:analysis; D013449:Sulfonamides / Q000032:analysis; D013997:Time Factors; D019155:Veterinary Drugs / Q000032:analysis* / Q000737:chemistry
chemical_list: D000871:Anthelmintics; D018942:Macrolides; D013449:Sulfonamides; D019155:Veterinary Drugs