title: A rapid method to determine sterol, erythrodiol, and uvaol concentrations in olive oil.
abstract: A rapid, accurate, and efficient method for determining the sterol, uvaol, and erythrodiol concentrations was developed to meet International Olive Council (IOC) certification criteria for extra virgin olive oil (EVOO). The unsaponifiable fraction of the sample (0.2 g) was separated with a diatomaceous earth column, and the sterol and triterpenic dialcohols were isolated with a novel base-activated silica solid-phase extraction (SPE) cartridge cleanup protocol. The improved method and the IOC method provided identical pass/fail results (n = 34) for each of the six sterol and erythrodiol/uvaol IOC criteria used to assess olive oil. This method was validated, and recoveries of stigmasterol (88%) and β-sitosterol (84%) were greater than previously published values obtained using the IOC method. This method requires approximately one-third the time required to complete the IOC method and has great utility for the rapid screening of EVOO to detect adulteration, false labeling, and an inferior product.
authors.affiliation: UC Analytical Laboratory, University of California (UC), 1 Shields Avenue, Davis, California 95616-5270, USA.
authors.forename: Brian
authors.initials: B
authors.lastname: Mathison
authors.forename: Dirk
authors.initials: D
authors.lastname: Holstege
mesh_terms: D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000032:analysis; D000069463:Olive Oil; D010938:Plant Oils / Q000032:analysis*; D012822:Silicon Dioxide; D052616:Solid Phase Extraction / Q000379:methods*; D013261:Sterols / Q000032:analysis*; D014315:Triterpenes / Q000032:analysis*
chemical_list: D000069463:Olive Oil; D010938:Plant Oils; D013261:Sterols; D014315:Triterpenes; C077953:erythrodiol; D009828:Oleanolic Acid; D012822:Silicon Dioxide; C013330:uvaol