title: Production of (3S)-acetoin from diacetyl by using stereoselective NADPH-dependent carbonyl reductase and glucose dehydrogenase.
abstract: Production of (3S)-acetoin ((3S)-AC), an important platform chemical, is desirable but difficult to perform. An NADPH-dependent carbonyl reductase (Gox0644) from Gluconobacter oxydans DSM 2003 was confirmed to have a good ability to reduce diacetyl (DA) to produce (3S)-AC. In this work, the NADPH-dependent carbonyl reductase was expressed and purified. Glucose dehydrogenase from Bacillus subtilis 168 was coupled with the NADPH-dependent carbonyl reductase to produce (3S)-AC from DA. Under the optimal conditions, 12.2 g l(-1) (3S)-AC was produced from 14.3 g l(-1) DA in 75 min. Because DA can be biotechnological produced, the two-enzymes coupling system might be a promising alternative for the (3S)-AC production.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China. jieerbu@sdu.edu.cn
authors.forename: Chao
authors.initials: C
authors.lastname: Gao
authors.forename: Lijie
authors.initials: L
authors.lastname: Zhang
authors.forename: Yingjian
authors.initials: Y
authors.lastname: Xie
authors.forename: Chunhui
authors.initials: C
authors.lastname: Hu
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.forename: Lixiang
authors.initials: L
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Cuiqing
authors.initials: C
authors.lastname: Ma
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
mesh_terms: D000093:Acetoin / Q000138:chemical synthesis*; D000429:Alcohol Oxidoreductases / Q000737:chemistry* / Q000235:genetics; D000449:Aldehyde Reductase; D000074408:Aldo-Keto Reductases; D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D004269:DNA, Bacterial; D003931:Diacetyl / Q000737:chemistry*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D020600:Gluconobacter oxydans / Q000235:genetics / Q000378:metabolism; D042843:Glucose 1-Dehydrogenase / Q000737:chemistry* / Q000235:genetics; D013237:Stereoisomerism
chemical_list: D004269:DNA, Bacterial; D000093:Acetoin; D000429:Alcohol Oxidoreductases; D000074408:Aldo-Keto Reductases; D000449:Aldehyde Reductase; D042843:Glucose 1-Dehydrogenase; D003931:Diacetyl