title: Electrochemiluminescence of blue-luminescent graphene quantum dots and its application in ultrasensitive aptasensor for adenosine triphosphate detection.
abstract: A simple approach based on exfoliating and disintegrating treatments for graphite oxide, followed by hydrothermal synthesis, was developed to prepare water-soluble graphene quantum dots (GQDs). The as-prepared GQDs exhibited bright blue emission under ultraviolet irradiation (∼365nm), and showed an excitation-independent photoluminescence feature. More importantly, a newly anodic electrochemiluminescence (ECL) was observed from the water-soluble GQDs with H2O2 as coreactant for the first time, and the ECL induced a strong light emission at a low potential (ca. 0.4V vs. Ag/AgCl). The ECL mechanism is investigated in detail. Employing SiO2 nanospheres as signal carrier, a novel SiO2/GQDs ECL signal amplification labels were synthesized based on which a ultrasensitive ECL aptamer sensor was proposed. Under the optimized experimental conditions, the proposed ECL aptamer sensor exhibited excellent analytical performance for adenosine triphosphate (ATP) determination, ranging from 5.0×10(-12) to 5.0×10(-9)molL(-1) with the detection limit of 1.5×10(-12)molL(-1). Due to the low cytotoxicity and excellent biocompatibility, GQDs are demonstrated to be an eco-friendly material as well as excellent ECL labeling agents for biosensor.
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Lu
authors.forename: Mei
authors.initials: M
authors.lastname: Yan
authors.forename: Lei
authors.initials: L
authors.lastname: Ge
authors.forename: Shenguang
authors.initials: S
authors.lastname: Ge
authors.forename: Shaowei
authors.initials: S
authors.lastname: Wang
authors.forename: Jixian
authors.initials: J
authors.lastname: Yan
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yu
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry / Q000302:isolation & purification*; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D006108:Graphite / Q000737:chemistry*; D049449:Luminescence; D045663:Quantum Dots / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D014867:Water; D006108:Graphite; D000255:Adenosine Triphosphate