title: Serological investigation of vector-borne disease in dogs from rural areas of China.
abstract: OBJECTIVE
  To evaluate the Anaplasma phagocytophilum (A. phagocytophilum), Ehrlichia canis (E. canis), Dirofilaria immitis (D. immitis) (canine heartworm), Borrelia burgdorferi (B. burgdorferi) infections in countryside dogs from Yunnan, Hainan and Anhui provinces.
  
  
  METHODS
  Serum samples were collected from 26 dogs in Yunnan, Hainan and Anhui provinces. The samples were tested using a commercial ELISA rapid diagnostic assay kit (SNAP(®) 4Dx(®); IDEXX Laboratories, Inc. U.S.A.). Meanwhile, indirect immunofluorescence assay (IFA) recommended by WHO was conducted to detect IgG to A. phagocytophilum. Two methods were analyzed and compared.
  
  
  RESULTS
  The number of serologically positive dogs for IgG to A. phagocytophilum was only 2 which was from Hainan province and none of the 26 dogs responded positive for E. canis, D. immitis (canine heartworm), and B. burgdorferi by ELISA rapid diagnostic method. The number of serologically positive dogs for IgG to A. phagocytophilum was 13 (50%) by IFA method. Data of the two methods were analyzed by statistical software and the difference was statistically significant (P=0.002).
  
  
  CONCLUSIONS
  It can be concluded that IFA method was more sensitive than ELISA rapid diagnostic method. However, we need conduct further and intensive epidemiology survey on tick-born diseases pathogens including A. phagocytophilum, E. canis, D. immitis (canine heartworm), and B. burgdorferi which have public health significance.
authors.affiliation: National Institute of Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijng 102206, China.
authors.forename: Shiwen
authors.initials: S
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: He
authors.forename: Lijuan
authors.initials: L
authors.lastname: Zhang
mesh_terms: D041081:Anaplasma phagocytophilum / Q000276:immunology; D000818:Animals; D025065:Borrelia burgdorferi / Q000276:immunology; D002681:China / Q000453:epidemiology; D004183:Dirofilaria immitis / Q000276:immunology; D004184:Dirofilariasis / Q000097:blood / Q000453:epidemiology* / Q000276:immunology; D004199:Disease Vectors; D004283:Dog Diseases / Q000453:epidemiology*; D004285:Dogs; D041061:Ehrlichia canis / Q000276:immunology; D016873:Ehrlichiosis / Q000097:blood / Q000453:epidemiology* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods; D007074:Immunoglobulin G / Q000097:blood; D008193:Lyme Disease / Q000097:blood / Q000453:epidemiology* / Q000276:immunology; D017282:Tick-Borne Diseases / Q000453:epidemiology*
chemical_list: D007074:Immunoglobulin G
keywords: Anaplasma phagocytophilum; Borrelia burgdorferi; China; Dirofilaria immitis; Dogs; ELISA rapid diagnostic assay; Ehrlichia canis; Serological investigation; Vector-borne disease