title: Is oxidative stress in mice brain regions diminished by 2-[(2,6-dichlorobenzylidene)amino]-5,6-dihydro-4H-cyclopenta[b]thiophene-3-carbonitrile?
abstract: 2-[(2,6-Dichlorobenzylidene)amino]-5,6-dihydro-4H-cyclopenta[b]thiophene-3-carbonitrile, 5TIO1, is a new 2-aminothiophene derivative with promising pharmacological activities. The aim of this study was to evaluate its antioxidant activity in different areas of mice central nervous system. Male Swiss adult mice were intraperitoneally treated with Tween 80 dissolved in 0.9% saline (control group) and 5TIO1 (0.1, 1, and 10 mg kg(-1)). Brain homogenates-hippocampus, striatum, frontal cortex, and cerebellum-were obtained after 24 h of observation. Superoxide dismutase and catalase activities, lipid peroxidation and nitrite content were measured using spectrophotometrical methods. To clarify the 5TIO1's mechanism on oxidative stress, western blot analysis of superoxide dismutase and catalase was also performed. 5TIO1 decreased lipid peroxidation and nitrite content in all brain areas and increased the antioxidant enzymatic activities, specially, in cerebellum. The data of Western blot analysis did not demonstrate evidence of the upregulation of these enzymes after the administration of this compound. Our findings strongly support that 5TIO1 can protect the brain against neuronal damages regularly observed during neuropathologies.
authors.affiliation: Postgraduate Program in Pharmaceutical Sciences, Federal University of PI, Teresina, Piauí, Brazil.
authors.forename: A C
authors.initials: AC
authors.lastname: Fortes
authors.forename: A A C
authors.initials: AA
authors.lastname: Almeida
authors.forename: G A L
authors.initials: GA
authors.lastname: Oliveira
authors.forename: P S
authors.initials: PS
authors.lastname: Santos
authors.forename: W
authors.initials: W
authors.lastname: De Lucca Junior
authors.forename: F J B
authors.initials: FJ
authors.lastname: Mendonça Junior
authors.forename: R M
authors.initials: RM
authors.lastname: Freitas
authors.forename: J L
authors.initials: JL
authors.lastname: Soares-Sobrinho
authors.forename: M F R
authors.initials: MF
authors.lastname: Soares
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D002531:Cerebellum / Q000201:enzymology / Q000378:metabolism; D003342:Corpus Striatum / Q000201:enzymology / Q000378:metabolism; D005625:Frontal Lobe / Q000201:enzymology / Q000378:metabolism; D006624:Hippocampus / Q000201:enzymology / Q000378:metabolism; D007274:Injections, Intraperitoneal; D015227:Lipid Peroxidation; D008297:Male; D051379:Mice; D009573:Nitrites / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D012545:Schiff Bases / Q000737:chemistry / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism; D013876:Thiophenes / Q000737:chemistry / Q000494:pharmacology*
chemical_list: C582901:2-((2,6-dichlorobenzylidene)amino)-5,6-dihydro-4H-cyclopenta(b)thiophene-3-carbonitrile; D000975:Antioxidants; D009573:Nitrites; D012545:Schiff Bases; D013876:Thiophenes; D002374:Catalase; D013482:Superoxide Dismutase