title: Rice and bean AHL-mimic quorum-sensing signals specifically interfere with the capacity to form biofilms by plant-associated bacteria.
abstract: Many bacteria regulate their gene expression in response to changes in their population density in a process called quorum sensing (QS), which involves communication between cells mediated by small diffusible signal molecules termed autoinducers. n-acyl-homoserine-lactones (AHLs) are the most common autoinducers in proteobacteria. QS-regulated genes are involved in complex interactions between bacteria of the same or different species and even with some eukaryotic organisms. Eukaryotes, including plants, can interfere with bacterial QS systems by synthesizing molecules that interfere with bacterial QS systems. In this work, the presence of AHL-mimic QS molecules in diverse Oryza sativa (rice) and Phaseolus vulgaris (bean) plant-samples were detected employing three biosensor strains. A more intensive analysis using biosensors carrying the lactonase enzyme showed that bean and rice seed-extract contain molecules that lack the typical lactone ring of AHLs. Interestingly, these molecules specifically alter the QS-regulated biofilm formation of two plant-associated bacteria, Sinorhizobium fredii SMH12 and Pantoea ananatis AMG501, suggesting that plants are able to enhance or to inhibit the bacterial QS systems depending on the bacterial strain. Further studies would contribute to a better understanding of plant-bacteria relationships at the molecular level.
authors.affiliation: Departamento de Microbiología, Facultad de Biología, Universidad de Sevilla, Avda. Reina Mercedes, 6, 41012 Sevilla, Spain. fperezm@us.es
authors.forename: Francisco
authors.initials: F
authors.lastname: Pérez-Montaño
authors.forename: Irene
authors.initials: I
authors.lastname: Jiménez-Guerrero
authors.forename: Rocío
authors.initials: R
authors.lastname: Contreras Sánchez-Matamoros
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: López-Baena
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: Ollero
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Rodríguez-Carvajal
authors.forename: Ramón A
authors.initials: RA
authors.lastname: Bellogín
authors.forename: M Rosario
authors.initials: MR
authors.lastname: Espuny
mesh_terms: D054742:Acyl-Butyrolactones / Q000737:chemistry / Q000378:metabolism*; D018441:Biofilms; D007887:Fabaceae / Q000737:chemistry / Q000378:metabolism* / Q000382:microbiology; D015394:Molecular Structure; D012275:Oryza / Q000737:chemistry / Q000378:metabolism* / Q000382:microbiology; D020636:Pantoea / Q000502:physiology*; D053147:Plant Exudates / Q000737:chemistry / Q000378:metabolism*; D053038:Quorum Sensing; D042181:Sinorhizobium fredii / Q000502:physiology*
chemical_list: D054742:Acyl-Butyrolactones; D053147:Plant Exudates
keywords: AHL mimic; Biofilm; Biosensor; Lactonase; Quorum quenching; Quorum sensing