title: Analgesic, anti-inflammatory and anti-diarrheal activities of ethanolic leaf extract of Typhonium trilobatum L. Schott.
abstract: OBJECTIVE
  To explore the efficacy of ethanolic leaf extract of Typhonium trilobatum L. Schott in treating diarrhea, pain and inflammation using experimental models.
  
  
  METHODS
  In the present study, acetic acid-induced writhing, xylene-induced ear edema and castor oil-induced diarrheal model were used to evaluate the analgesic, anti-inflammatory and anti-diarrheal activities, respectively. Acute toxicity test was carried out to fix the safe doses of the plant extract.
  
  
  RESULTS
  The plant extract demonstrated a significant inhibition of writhing (P<0.01) compared with the control group in acetic acid-induced writhing test in mice. The extract also significantly inhibited the xylene induced ear edema formation (P<0.05). In anti-diarrheal test, the extract significantly decreased the frequency of defecation and increased the mean latent period (P<0.01) in castor oil-induced diarrheal model mice at the doses of 250 and 500 mg/kg body weight.
  
  
  CONCLUSIONS
  These results suggest that the extract possesses significant analgesic, anti-inflammatory and anti-diarrheal activities that support to the ethnopharmacological uses of this plant.
authors.affiliation: Biotechnology Department, Biological Science School, Chung Ang University, Ansung Kyunggido 456-756, Republic of South Korea ; Biotechnology and Genetic Engineering Discipline, Life Science School, Khulna University, Khulna-9208, Bangladesh.
authors.forename: Khadem
authors.initials: K
authors.lastname: Ali
authors.forename: Ayesha
authors.initials: A
authors.lastname: Ashraf
authors.forename: Nripendra
authors.initials: N
authors.lastname: Nath Biswas
mesh_terms: D000700:Analgesics / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D000930:Antidiarrheals / Q000737:chemistry / Q000494:pharmacology*; D003967:Diarrhea / Q000139:chemically induced / Q000188:drug therapy; D004195:Disease Models, Animal; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D005260:Female; D019684:Magnoliopsida / Q000737:chemistry*; D008297:Male; D051379:Mice; D064209:Phytochemicals / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D051381:Rats; D023382:Toxicity Tests, Acute
chemical_list: D000700:Analgesics; D000893:Anti-Inflammatory Agents; D000930:Antidiarrheals; D064209:Phytochemicals; D010936:Plant Extracts
keywords: Analgesic; Anti-diarrheal; Anti-inflammatory; Toxicity; Typhonium trilobatum