title: In vitro direct shoot regeneration from proximal, middle and distal segment of Coleus forskohlii leaf explants.
abstract: Coleus forskohlii is an endangered multipurpose medicinal plant that has widespread applications. In spite of this, there have been few attempts to propagate its cultivation in India. The present communication presents an in vitro rapid regeneration method using leaf explants of Coleus forskohlii through direct organogenesis. Leaf explants that were excised into three different segments i.e. proximal (P), middle (M) and distal (D) were cultured on Murashige and Skoog (MS) basal medium supplemented with cytokinins. MS Media containing 5.0 mg L(-1) BAP (6-Benzylaminopurine) promoted regeneration of multiple shoots through direct organogenesis from the leaf, which were further elongated on MS media augmented with 0.1 mg L-1 BAP and 0.1 mg L(-1) IAA (Indole-3-acetic acid), cytokinin and auxin combination. Regenerated and elongated shoots, when transferred to ose resulted in profuse rooting plants that were transferred to soil after acclimatization and maintained in a green house. The current protocol offers a direct, mass propagation method bypassing the callus phase of C. forskohlii and is suitable for conservation, large-scale commercial cultivation, and genetic transformation with agronomically desirable traits.
authors.affiliation: College of Biotechnology & Allied Sciences, Allahabad Agricultural Institute Deemed University, Allahabad, 211 007 UP India ; Biotechnology Division, JK Agri Genetics Ltd., 1-10-177, 4th Floor, Varun Towers, Begumpet, Hyderabad, 500 016 AP India.
authors.forename: Gaurav
authors.initials: G
authors.lastname: Krishna
authors.forename: P
authors.initials: P
authors.lastname: Sairam Reddy
authors.forename: N
authors.initials: N
authors.lastname: Anoop Nair
authors.forename: P W
authors.initials: PW
authors.lastname: Ramteke
authors.forename: P S
authors.initials: PS
authors.lastname: Bhattacharya
keywords: 6-benzylaminopurine; Coleus forskohlii; cytokinin; multiple shoots; tissue culture