title: Aerobic methane oxidation coupled to denitrification in a membrane biofilm reactor: treatment performance and the effect of oxygen ventilation.
abstract: Aerobic methane-oxidation coupled to denitrification (AME-D) process was successfully achieved in a membrane biofilm reactor (MBfR). PVDF membrane was employed to supply the methane and oxygen for biofilm, which was coexistence of methanotrophs and denitrifier. With a feeding NO3(-)-N of 30 mg/L, up to 97% nitrate could be removed stably. The oxygen ventilation modes impacted the denitrification performance remarkably, resulting in different nitrate removal efficiencies and biofilm microorganism distribution. The biofilm sludge showed a high resistance to the DO inhibition, mainly due to the co-existing methanotroph being capable of utilizing oxygen perferentially within biofilm, and create an anoxic micro-environment. The denitrification of both nitrate and nitrite by biofilm sludge conformed to the Monod equation, and the maximum specific nitrate utilization rate (k) ranged from 1.55 to 1.78 NO3(-)-N/g VSS-d. The research findings should be significant to understand the considerable potential of MBfR as a bioprocess for denitrification.
authors.affiliation: ShenZhen Key Laboratory of Water Resource Application and Environmental Pollution Control, Harbin Institute of Technology Shenzhen Graduate School, Shenzhen 518055, China. sunfeiyun1982@gmail.com
authors.forename: Fei-yun
authors.initials: FY
authors.lastname: Sun
authors.forename: Wen-yi
authors.initials: WY
authors.lastname: Dong
authors.forename: Ming-fei
authors.initials: MF
authors.lastname: Shao
authors.forename: Xiao-mei
authors.initials: XM
authors.lastname: Lv
authors.forename: Ji
authors.initials: J
authors.lastname: Li
authors.forename: Liang-yu
authors.initials: LY
authors.lastname: Peng
authors.forename: Hong-jie
authors.initials: HJ
authors.lastname: Wang
mesh_terms: D018441:Biofilms; D019149:Bioreactors; D017931:DNA Primers / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D058440:Denitrification / Q000502:physiology*; D059014:High-Throughput Nucleotide Sequencing; D008567:Membranes, Artificial; D008697:Methane / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D011145:Polyvinyls; D020560:Proteobacteria / Q000235:genetics / Q000378:metabolism*; D005465:Fluorocarbon Polymers
chemical_list: D017931:DNA Primers; D008567:Membranes, Artificial; D008697:Methane; D010100:Oxygen; D011145:Polyvinyls; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers
keywords: AME-D; Aerobic methane-oxidation; Biological nitrogen removal; Denitrification