title: A label-free, quantitative assay of amyloid fibril growth based on intrinsic fluorescence.
abstract: Kinetic assay of seeded growth: The graph shows the variation in intrinsic fluorescence intensity of amyloid fibrils. Fluorescence increases during the seeded aggregation of α-synuclein seeds with α-synuclein monomeric protein (blue curve) but not when α-synuclein seeds are incubated with β-synuclein monomeric protein (black curve), thus showing that no seeded growth occurred in this case.
authors.affiliation: Department of Chemical Engineering and Biotechnology, University of Cambridge, Pembroke Street, Cambridge, CB2 3RA, UK.
authors.forename: Dorothea
authors.initials: D
authors.lastname: Pinotsi
authors.forename: Alexander K
authors.initials: AK
authors.lastname: Buell
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Dobson
authors.forename: Gabriele S
authors.initials: GS
authors.lastname: Kaminski Schierle
authors.forename: Clemens F
authors.initials: CF
authors.lastname: Kaminski
mesh_terms: D016229:Amyloid beta-Peptides / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D052160:Benzothiazoles; D005453:Fluorescence; D006801:Humans; D007700:Kinetics; D010316:Particle Size; D010446:Peptide Fragments / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D013844:Thiazoles / Q000737:chemistry
chemical_list: D016229:Amyloid beta-Peptides; D052160:Benzothiazoles; D010446:Peptide Fragments; D013844:Thiazoles; C075222:amyloid beta-protein (1-42); C009462:thioflavin T