title: Identifying serum biomarkers for ovarian cancer by screening with surface-enhanced laser desorption/ionization mass spectrometry and the artificial neural network.
abstract: OBJECTIVE
  The purpose of this study was to screen potential serum tumor biomarkers for the diagnosis of ovarian cancer.
  
  
  METHODS
  The study includes 3 sets. The first set of patients included 37 ovarian cancers and 31 healthy women (healthy controls). The second set included 42 ovarian cancers, 33 patients with benign ovarian tumor, and 29 healthy women (noncancer controls). The third set included 39 ovarian cancers and 35 patients with benign ovarian tumor (benign controls). Serum samples from ovarian cancers, healthy controls, noncancer controls, and benign controls were analyzed by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry.
  
  
  RESULTS
  A 3-peak model (peaks of mass-to-charge ratio values at 5766.379 d, 5912.586 d, and 11695.56 d) was established in the training set that discriminated cancer from noncancer with high sensitivity (10/11, 90.90%) and specificity (19/20, 95.00%). The peaks corresponding to 3 potential biomarkers increased significantly with the degree of malignancy.
  
  
  CONCLUSIONS
  The proteins represented by these 3 peaks are biomarker candidates for ovarian cancer diagnosis and/or monitoring treatment response.
authors.affiliation: Department of Obstetrics and Gynecology, Haidian Women's and Children's Hospital, Beijing 100080, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Zhu
authors.forename: Hongyan
authors.initials: H
authors.lastname: Guo
authors.forename: Xiuyun
authors.initials: X
authors.lastname: Wang
authors.forename: Ronglian
authors.initials: R
authors.lastname: Gao
authors.forename: Lufang
authors.initials: L
authors.lastname: Zhang
authors.forename: Yangyu
authors.initials: Y
authors.lastname: Zhao
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000097:blood*; D002277:Carcinoma / Q000097:blood* / Q000175:diagnosis; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008875:Middle Aged; D016571:Neural Networks, Computer; D010051:Ovarian Neoplasms / Q000097:blood* / Q000175:diagnosis; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015854:Up-Regulation / Q000502:physiology*
chemical_list: D014408:Biomarkers, Tumor