title: Statistical key factors optimization of conditions for hydrogen production from S-TE (solubilization by thermophilic enzyme) waste sludge.
abstract: Waste sludge can be solubilized after S-TE (solubilization by thermophilic enzyme) pretreatment as the cryptic growth occurs at the expense of the cell lysate. The hydrogen production from S-TE sludge is greatly influenced by many factors. In this study, factors including pH, C/N, C/P, and Fe(2+) affecting hydrogen production from S-TE sludge were optimized using uniform design. The optimum condition for maximum hydrogen yield of 68.4 ml H2/g VSS (volatile suspended solid) could be predicted from regression model, and the optimum conditions were pH of 6.4, C/N ratio of 38, C/P ratio of 265, and Fe(2+) concentration of 85 mg/L. There was interaction effect of factors on hydrogen production from S-TE sludge. Different pH, C/N, C/P and Fe(2+) conditions could influence the VSS removal rate, carbohydrate and protein utilization. When the highest compositions of acetate and ethanol and lowest propionate were observed in metabolites, effective hydrogen production was also achieved.
authors.affiliation: Key Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China. geletu@ouc.edu.cn
authors.forename: Liang
authors.initials: L
authors.lastname: Guo
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
authors.forename: Zonglian
authors.initials: Z
authors.lastname: She
authors.forename: Mingmin
authors.initials: M
authors.lastname: Lu
authors.forename: Yan
authors.initials: Y
authors.lastname: Zong
mesh_terms: D019149:Bioreactors; D002244:Carbon / Q000737:chemistry; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D009584:Nitrogen / Q000737:chemistry; D010710:Phosphates; D012722:Sewage / Q000737:chemistry*; D012995:Solubility
chemical_list: D010710:Phosphates; D012722:Sewage; D002244:Carbon; D006859:Hydrogen; D009584:Nitrogen