title: Dynamin-SNARE interactions control trans-SNARE formation in intracellular membrane fusion.
abstract: The fundamental processes of membrane fission and fusion determine size and copy numbers of intracellular organelles. Although SNARE proteins and tethering complexes mediate intracellular membrane fusion, fission requires the presence of dynamin or dynamin-related proteins. Here we study these reactions in native yeast vacuoles and find that the yeast dynamin homologue Vps1 is not only an essential part of the fission machinery, but also controls membrane fusion by generating an active Qa SNARE-tethering complex pool, which is essential for trans-SNARE formation. Our findings provide new insight into the role of dynamins in membrane fusion by directly acting on SNARE proteins.
authors.affiliation: Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA.
authors.forename: Kannan
authors.initials: K
authors.lastname: Alpadi
authors.forename: Aditya
authors.initials: A
authors.lastname: Kulkarni
authors.forename: Sarita
authors.initials: S
authors.lastname: Namjoshi
authors.forename: Sankaranarayanan
authors.initials: S
authors.lastname: Srinivasan
authors.forename: Katherine H
authors.initials: KH
authors.lastname: Sippel
authors.forename: Kathryn
authors.initials: K
authors.lastname: Ayscough
authors.forename: Martin
authors.initials: M
authors.lastname: Zieger
authors.forename: Andrea
authors.initials: A
authors.lastname: Schmidt
authors.forename: Andreas
authors.initials: A
authors.lastname: Mayer
authors.forename: Michael
authors.initials: M
authors.lastname: Evangelista
authors.forename: Florante A
authors.initials: FA
authors.lastname: Quiocho
authors.forename: Christopher
authors.initials: C
authors.lastname: Peters
mesh_terms: D034281:Dynamins / Q000378:metabolism* / Q000502:physiology; D008561:Membrane Fusion; D011485:Protein Binding; D050600:SNARE Proteins / Q000378:metabolism* / Q000502:physiology
chemical_list: D050600:SNARE Proteins; D034281:Dynamins