title: Identification of the outer-inner dynein linker as a hub controller for axonemal dynein activities.
abstract: BACKGROUND
  In flagella, the outer dynein arm (ODA) and inner dynein arm (IDA) play distinct roles in generating beating motion. However, functional communications between the two dyneins have not been investigated.
  
  
  RESULTS
  Here, we demonstrated by cryo-electron microscopy and chemical crosslinking that intermediate chain 2 (IC2) of ODAs interacts with the dynein regulatory complex in the axoneme and constitutes part of the outer-inner dynein (OID) linker. Furthermore, we identified IC2 as a functional hub between ODAs and IDAs based on the phenotypes of Chlamydomonas mutants expressing biotinylation-tagged IC2. The flagella of the IC2 mutant showed activated microtubule sliding and enhanced ATPase activities of ODAs, as well as an altered waveform, indicating attenuated IDA activity.
  
  
  CONCLUSIONS
  We concluded that the OID linker controls both ODAs and IDAs and regulates flagellar beating.
authors.affiliation: Department of Cell Biology and Anatomy, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Oda
authors.forename: Toshiki
authors.initials: T
authors.lastname: Yagi
authors.forename: Haruaki
authors.initials: H
authors.lastname: Yanagisawa
authors.forename: Masahide
authors.initials: M
authors.lastname: Kikkawa
mesh_terms: D056444:Axonemal Dyneins / Q000235:genetics / Q000378:metabolism*; D019921:Biotinylation; D002696:Chlamydomonas / Q000166:cytology* / Q000235:genetics / Q000378:metabolism*; D020285:Cryoelectron Microscopy; D005407:Flagella / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism; D010641:Phenotype
chemical_list: D056444:Axonemal Dyneins