title: Nanotips for single-step preparation of DNA for qPCR analysis.
abstract: A single-step concentration and elution method is developed for detection of DNA in buffer, saliva, and blood. A nanotip capturing DNA using an electric field and capillary action is directly dissolved in buffer for qPCR analysis. The concentration yield and the relative parameters are compared with those of a commercial kit.
authors.affiliation: Department of Mechanical Engineering, University of Washington, Seattle, WA, USA.
authors.forename: Dinesh
authors.initials: D
authors.lastname: Kalyanasundaram
authors.forename: Jong-Hoon
authors.initials: JH
authors.lastname: Kim
authors.forename: Gareth
authors.initials: G
authors.lastname: Fotouhi
authors.forename: Hyun-Boo
authors.initials: HB
authors.lastname: Lee
authors.forename: Morgan
authors.initials: M
authors.lastname: Hiraiwa
authors.forename: Kieseok
authors.initials: K
authors.lastname: Oh
authors.forename: Kyong-Hoon
authors.initials: KH
authors.lastname: Lee
authors.forename: Jae-Hyun
authors.initials: JH
authors.lastname: Chung
mesh_terms: D053000:Analytic Sample Preparation Methods / Q000379:methods*; D010582:Bacteriophage lambda / Q000235:genetics; D017554:Carbon Compounds, Inorganic / Q000737:chemistry; D004247:DNA / Q000032:analysis / Q000097:blood / Q000235:genetics* / Q000302:isolation & purification*; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D015894:Genome, Human / Q000235:genetics; D006801:Humans; D036103:Nanotechnology / Q000379:methods*; D053770:Nanowires / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods*; D012463:Saliva / Q000737:chemistry; D017655:Silicon Compounds / Q000737:chemistry; D013997:Time Factors
chemical_list: D017554:Carbon Compounds, Inorganic; D004279:DNA, Viral; D017655:Silicon Compounds; D004247:DNA; C022088:silicon carbide