title: First identification of novel NDM carbapenemase, NDM-7, in Escherichia coli in France.
abstract: BACKGROUND
  The NDM-1 carbapenemase has been identified in 2008 in Enterobacteriaceae. Since then, several reports have emphasized its rapid dissemination throughout the world. The spread of NDM carbapenemases involve several bla NDM gene variants associated with various plasmids among several Gram negative species.
  
  
  METHODS
  A multidrug-resistant E. coli isolate recovered from urine of a patient who had travelled to Burma has been characterized genetically and biochemically.
  
  
  RESULTS
  E. coli COU was resistant to all antibiotics tested except amikacin, tigecycline, fosfomycin, and chloramphenicol. Analysis of the antibiotic resistance traits identified a metallo-ß-lactamase, a novel NDM variant, NDM-7. It differs from NDM-4 by a single amino acid substitution sharing an identical extended spectrum profile towards carbapenems. The bla NDM-7 gene was located on an untypeable conjugative plasmid and associated with a close genetic background similar to those described among the bla NDM-1 genes. The isolate also harbours bla CTXM-15 and bla OXA-1 genes and belonged to ST167.
  
  
  CONCLUSIONS
  This study highlights that spread of NDM producers correspond to spread of multiple bla NDM genes and clones and therefore will be difficult to control.
authors.affiliation: Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, INSERM U914, Faculté de Médecine Paris-Sud, France.
authors.forename: Gaelle
authors.initials: G
authors.lastname: Cuzon
authors.forename: Rémy A
authors.initials: RA
authors.lastname: Bonnin
authors.forename: Patrice
authors.initials: P
authors.lastname: Nordmann
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology*; D005602:France; D005798:Genes, Bacterial / Q000235:genetics; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D050505:Mutant Proteins / Q000737:chemistry / Q000378:metabolism; D016415:Sequence Alignment; D001618:beta-Lactamases / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D050505:Mutant Proteins; D001618:beta-Lactamases; C063912:carbapenemase