title: Optimization of water-in-oil-in-water microencapsulated β-galactosidase by response surface methodology.
abstract: This study was carried out to determine the optimum conditions for water-in-oil-in-water (W/O/W) microencapsulated lactase (β-galactosidase) in order to prevent the intolerance of lactose in milk. The core material was lactase and the coating materials were medium-chain triglyceride for W/O phase, and whey protein isolate (WPI), maltodextrin, gum arabic, and its mixtures for W/O/W phase. Polyglycerol polyricinoleate (PGPR) was used as a primary emulsifier, and polyoxyethylene sorbitan monolaurate (PSML) was selected as a secondary emulsifier based on emulsion stability index. To determine the most efficient conditions for the W/O/W-lactase microencapsulation, the ratio of core to coating materials and amounts of emulsifiers were investigated by response surface methodology. The optimum ratio of core to coating materials in W/O, amount of PGPR, ratio of core to coating material in W/O/W, and amount of PSML were found to be 0.5-9.5, 0.75% (w/v), 1.7-8.3, and 0.25% (w/v), respectively. The average size of the microcapsules was about 10 µm under optimum conditions. Microcapsules of 30% (w/v) WPI as a secondary coating material could evenly distribute the pocket of lactase. Based on the data obtained from this study, lactase microcapsules could effectively be produced by the method of W/O/W double emulsion.
authors.affiliation: Department of Food Science and Technology, Sejong University, Seoul 143-747, Korea.
authors.forename: Sung-Il
authors.initials: SI
authors.lastname: Ahn
authors.forename: Yun-Kyung
authors.initials: YK
authors.lastname: Lee
authors.forename: Hae-Soo
authors.initials: HS
authors.lastname: Kwak
mesh_terms: D002214:Capsules; D004339:Drug Compounding; D004655:Emulsions / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism; D005990:Glycerol / Q000031:analogs & derivatives / Q000737:chemistry; D006170:Gum Arabic / Q000737:chemistry; D006801:Humans; D007785:Lactose / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry; D011136:Polysorbates / Q000737:chemistry; D012277:Ricinoleic Acids / Q000737:chemistry; D014280:Triglycerides / Q000737:chemistry; D014867:Water / Q000737:chemistry; D001616:beta-Galactosidase / Q000008:administration & dosage* / Q000737:chemistry / Q000378:metabolism
chemical_list: D002214:Capsules; D004655:Emulsions; D004800:Enzymes, Immobilized; D011134:Polysaccharides; D011136:Polysorbates; D012277:Ricinoleic Acids; D014280:Triglycerides; C029243:glyceran polyricinoleic acid ester; D014867:Water; C008315:maltodextrin; D006170:Gum Arabic; D001616:beta-Galactosidase; D007785:Lactose; D005990:Glycerol