title: The novel BTB-kelch protein, KBTBD8, is located in the Golgi apparatus and translocates to the spindle apparatus during mitosis.
abstract: Proteins of the BTB-kelch family are known to be involved in multiple biological processes such as migration, cytoskeleton arrangement, regulation of cell morphology, protein ubiquitination and gene expression. KBTBD8 is a new member of this family. The gene was found in a comparative transcriptome analysis of pluripotent stem cells and was therefore suggested to play a role in the regulation of pluripotency. Comparative analysis of the gene and protein sequences revealed a high conservation throughout evolution especially in the characteristic domains of BTB, BACK and kelch. We identified the Golgi apparatus as the subcellular localization of the KBTBD8 protein in non-dividing cells and could show that KBTBD8 co-localizes with α-tubulin on the spindle apparatus of mitotic cells suggesting a role in cell proliferation. In conclusion, KBTBD8 is a new member of the BTB-kelch superfamily that is located in the Golgi apparatus and translocates to the spindle apparatus during mitosis.
authors.affiliation: Institute of Human Genetics, University of Göttingen, Göttingen, 37073, Germany. jnolte1@gwdg.de.
authors.forename: Sandra
authors.initials: S
authors.lastname: Lührig
authors.forename: Susanne
authors.initials: S
authors.lastname: Kolb
authors.forename: Nadine
authors.initials: N
authors.lastname: Mellies
authors.forename: Jessica
authors.initials: J
authors.lastname: Nolte