title: In vivo antioxidant and hepatoprotective activity of methanolic extracts of Daucus carota seeds in experimental animals.
abstract: OBJECTIVE
  To assess the In vivo antioxidFant and hepatoprotective activity of methanolic extract of Daucus carota (D. carota) seeds in experimental animals.
  
  
  METHODS
  Methanolic extracts of D. carota seeds is used for hepatoprotection assessment. Oxidative stress were induced in rats by thioacetamide 100 mg/kg s.c, in four groups of rats (two test, standard and toxic control). Two test groups received D. carota seeds extract (DCSE) at doses of 200 mg/kg and 400 mg/kg. Standard group received silymarin (25 mg/kg) and toxic control received only thioacetamide. Control group received only vehicle. On the 8th day animals were sacrificed and liver enzyme like serum glutamic pyruvic transaminase (SGPT), serum glutamic-oxaloacetic transaminase (SGOT) and alkaline phosphatase (ALP) were estimated in blood serum and antioxidant enzyme like superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GRD), glutathione peroxidase (GPX), glutathione-S-transferase (GST) and lipid peroxidation (LPO) were estimated in liver homogenate.
  
  
  RESULTS
  A significant decrease in SGPT, SGOT and ALP levels was observed in all drug treated groups as compared to thioacetamide group (P < 0.001) and in case of antioxidant enzyme a significant (P < 0.001) increase in SOD, CAT, GRD, GPX and GST was observed in all drug treated groups as compared with thioacetamide group. But in case of LPO a significant (P < 0.001) reduction was observed as compared to toxic control group.
  
  
  CONCLUSIONS
  DCSE has contributed to the reduction of oxidative stress and the protection of liver in experimental rats.
authors.affiliation: Rawatpura Sarkar Institute of Pharmacy, Raipur, India.
authors.forename: Kamlesh
authors.initials: K
authors.lastname: Singh
authors.forename: Nisha
authors.initials: N
authors.lastname: Singh
authors.forename: Anish
authors.initials: A
authors.lastname: Chandy
authors.forename: Ashish
authors.initials: A
authors.lastname: Manigauha
mesh_terms: D000410:Alanine Transaminase; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001219:Aspartate Aminotransferases; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000473:pathology; D018552:Daucus carota / Q000737:chemistry*; D004195:Disease Models, Animal; D005978:Glutathione; D005979:Glutathione Peroxidase; D015227:Lipid Peroxidation; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D000432:Methanol; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy / Q000379:methods; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D012639:Seeds / Q000737:chemistry*; D013482:Superoxide Dismutase; D013853:Thioacetamide
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D013853:Thioacetamide; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D005978:Glutathione; D000432:Methanol
keywords: Antioxidant; Biochemical parameters; Daucus carota seeds extract; Hepatoprotection; Silymarin; Thioacetamide