title: HIV-1 envelope glycoprotein trimers display open quaternary conformation when bound to the gp41 membrane-proximal external-region-directed broadly neutralizing antibody Z13e1.
abstract: We describe cryo-electron microscopic studies of the interaction between the ectodomain of the trimeric HIV-1 envelope glycoprotein (Env) and Z13e1, a broadly neutralizing antibody that targets the membrane-proximal external region (MPER) of the gp41 subunit. We show that Z13e1-bound Env displays an open quaternary conformation similar to the CD4-bound conformation. Our results support the idea that MPER-directed antibodies, such as Z13e1, block viral entry by interacting with Env at a step after CD4 activation.
authors.affiliation: Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, NIH, Bethesda, Maryland, USA.
authors.forename: Audray K
authors.initials: AK
authors.lastname: Harris
authors.forename: Alberto
authors.initials: A
authors.lastname: Bartesaghi
authors.forename: Jacqueline L S
authors.initials: JL
authors.lastname: Milne
authors.forename: Sriram
authors.initials: S
authors.lastname: Subramaniam
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D057134:Antibodies, Neutralizing / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D020285:Cryoelectron Microscopy; D015700:HIV Envelope Protein gp41 / Q000276:immunology*; D006801:Humans; D008958:Models, Molecular; D009500:Neutralization Tests; D011485:Protein Binding; D011487:Protein Conformation; D055503:Protein Multimerization; D054299:env Gene Products, Human Immunodeficiency Virus / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D015700:HIV Envelope Protein gp41; D054299:env Gene Products, Human Immunodeficiency Virus; C514787:gp41 protein, Human immunodeficiency virus 1