title: The closterovirus-derived gene expression and RNA interference vectors as tools for research and plant biotechnology.
abstract: Important progress in understanding replication, interactions with host plants, and evolution of closteroviruses enabled engineering of several vectors for gene expression and virus-induced gene silencing. Due to the broad host range of closteroviruses, these vectors expanded vector applicability to include important woody plants such as citrus and grapevine. Furthermore, large closterovirus genomes offer genetic capacity and stability unrivaled by other plant viral vectors. These features provided immense opportunities for using closterovirus vectors for the functional genomics studies and pathogen control in economically valuable crops. This review briefly summarizes advances in closterovirus research during the last decade, explores the relationships between virus biology and vector design, and outlines the most promising directions for future application of closterovirus vectors.
authors.affiliation: Department of Botany and Plant Pathology, Oregon State University Corvallis, OR, USA ; Center for Genome Research and Biocomputing, Oregon State University Corvallis, OR, USA.
authors.forename: Valerian V
authors.initials: VV
authors.lastname: Dolja
authors.forename: Eugene V
authors.initials: EV
authors.lastname: Koonin
keywords: Beet yellows virus; Citrus tristeza virus; Grapevine leafroll-associated virus-2; RNAi; closteroviruses; viral vector