title: Preparation of chitosan-graft-(β-cyclodextrin) based sol-gel stationary phase for open-tubular capillary electrochromatography.
abstract: A novel open-tubular CEC column coated with chitosan-graft-(β-CD) (CDCS) was prepared using sol-gel technique. In the sol-gel approach, owing to the 3D network of sol-gel and the strong chemical bond between the stationary phase and the surface of capillary columns, good chromatographic characteristics and unique selectivity in separating isomers were shown. The column efficiencies of 55,000∼163,000 plates/m for the isomeric xanthopterin and phenoxy acid herbicides using the sol-gel-derived CDCS columns were achieved. Good stabilities were demonstrated that the RSD values for the retention time of thiourea and isoxanthopterin were 1.3 and 1.4% (run to run, n = 5), 1.6 and 2.0% (day to day, n = 3), 2.9 and 3.1% (column to column, n = 3), respectively. The sol-gel-coated CDCS columns have shown improved separations of isomeric xanthopterin in comparison with CDCS-bonded capillary column.
authors.affiliation: College of Materials Science and Engineering, Fuzhou University, Fuzhou, P. R. China. hx_lv@163.com
authors.forename: Haixia
authors.initials: H
authors.lastname: Lü
authors.forename: Qingyin
authors.initials: Q
authors.lastname: Li
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Yu
authors.forename: Jiaojiao
authors.initials: J
authors.lastname: Yi
authors.forename: Zenghong
authors.initials: Z
authors.lastname: Xie
mesh_terms: D053801:Capillary Electrochromatography / Q000295:instrumentation* / Q000379:methods; D048271:Chitosan / Q000737:chemistry*; D053841:Electroosmosis; D006540:Herbicides / Q000737:chemistry / Q000302:isolation & purification; D006863:Hydrogen-Ion Concentration; D007536:Isomerism; D008956:Models, Chemical; D044367:Phase Transition; D015203:Reproducibility of Results; D014976:Xanthopterin / Q000737:chemistry / Q000302:isolation & purification; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D006540:Herbicides; D047392:beta-Cyclodextrins; D048271:Chitosan; D014976:Xanthopterin
keywords: Chitosan; Open-tubular capillary electrochromatography; Sol-gel technique; β-Cyclodextrin