title: Associations between single-nucleotide polymorphisms and epidural ropivacaine consumption in patients undergoing breast cancer surgery.
abstract: Up to date, few published studies indicated the associations between genetic polymorphisms and epidural local anesthetics consumption. In this study, we investigated the associations between seven single-nucleotide polymorphisms (SNPs) and epidural ropivacaine consumption during breast cancer surgery in women from northeastern China. These seven SNPs (rs3803662 and rs12443621 in TNCR9, rs889312 in MAP3K1, rs3817198 in LSP1, rs13387042 at 2q35, rs13281615 at 8q24, and rs2046210 at 6q25.1) were identified by recent genome-wide association studies associated with tumor susceptibility. A total of 418 breast cancer women received thoracic epidural anesthesia with ropivacaine for elective mastectomy with axillary clearance. Their blood samples were genotyped for the seven SNPs using the SNaPshot method. For SNP rs13281615, the subjects with genotype AG and GG consumed a greater amount of the total epidural ropivacaine and the mean ropivacaine dose than the subjects with genotype AA (p=0.047 and p=0.003, respectively). Furthermore, no statistical differences were found in the total dose of ropivacaine, the mean consumption of ropivacaine, the onset of ropivacaine, or the initial dose of lidocaine among the three genotypic groups for the other six SNPs studied. Our study indicated that SNP rs13281615 at 8q24 was associated with the consumption of epidural ropivacaine during breast cancer surgery in northeastern Chinese women. It might provide new insights into the mechanisms of ropivacaine action and metabolism and facilitate the development of personalized medicine.
authors.affiliation: Department of Anesthesiology, The Second Affiliated Hospital, Harbin Medical University, Harbin, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Yongdong
authors.initials: Y
authors.lastname: Jiang
authors.forename: Da
authors.initials: D
authors.lastname: Pang
authors.forename: Hongjie
authors.initials: H
authors.lastname: Xi
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Wenzhi
authors.initials: W
authors.lastname: Li
mesh_terms: D000328:Adult; D000577:Amides / Q000008:administration & dosage*; D000767:Anesthesia, Epidural; D000779:Anesthetics, Local / Q000008:administration & dosage*; D001943:Breast Neoplasms / Q000235:genetics* / Q000601:surgery; D002681:China; D005260:Female; D056726:Genetic Association Studies; D006801:Humans; D048728:MAP Kinase Kinase Kinase 1 / Q000235:genetics; D008408:Mastectomy; D008840:Microfilament Proteins / Q000235:genetics; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D000077212:Ropivacaine
chemical_list: D000577:Amides; D000779:Anesthetics, Local; C489183:LSP1 protein, human; D008840:Microfilament Proteins; D000077212:Ropivacaine; D048728:MAP Kinase Kinase Kinase 1; C482576:MAP3K1 protein, human