title: Patterned polymeric surfaces to study the influence of nanotopography on the growth and differentiation of mesenchymal stem cells.
abstract: The implementation of micro- and nanotechnologies to biomaterials constitutes a unique platform to improve our understanding on microenvironmental regulation of stem cell functions. In the recent years, various methods have been developed for the fabrication of micro- and nanopatterned polymeric culture substrates, and many of these novel surfaces are opening possibilities for new applications. Here, we provide procedures for creating nanoscale topographic features on films of poly(lactic acid), a biodegradable polymer frequently used for the fabrication of tissue engineering scaffolds. In addition, we provide methods to assess the growth and differentiation of mesenchymal stem cells cultured on the substrates.
authors.affiliation: Laboratory for Stem Cell Research, Department of Health Science and Technology, Aalborg University, Aalborg, Denmark.
authors.forename: Cristian Pablo
authors.initials: CP
authors.lastname: Pennisi
authors.forename: Vladimir
authors.initials: V
authors.lastname: Zachar
authors.forename: Trine
authors.initials: T
authors.lastname: Fink
authors.forename: Leonid
authors.initials: L
authors.lastname: Gurevich
authors.forename: Peter
authors.initials: P
authors.lastname: Fojan
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D049109:Cell Proliferation; D005455:Fluorescent Antibody Technique; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D036103:Nanotechnology; D011108:Polymers / Q000737:chemistry; D060888:Real-Time Polymerase Chain Reaction; D013499:Surface Properties; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D011108:Polymers