title: Robust and highly-efficient differentiation of functional monocytic cells from human pluripotent stem cells under serum- and feeder cell-free conditions.
abstract: Monocytic lineage cells (monocytes, macrophages and dendritic cells) play important roles in immune responses and are involved in various pathological conditions. The development of monocytic cells from human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) is of particular interest because it provides an unlimited cell source for clinical application and basic research on disease pathology. Although the methods for monocytic cell differentiation from ESCs/iPSCs using embryonic body or feeder co-culture systems have already been established, these methods depend on the use of xenogeneic materials and, therefore, have a relatively poor-reproducibility. Here, we established a robust and highly-efficient method to differentiate functional monocytic cells from ESCs/iPSCs under serum- and feeder cell-free conditions. This method produced 1.3 × 10(6) ± 0.3 × 10(6) floating monocytes from approximately 30 clusters of ESCs/iPSCs 5-6 times per course of differentiation. Such monocytes could be differentiated into functional macrophages and dendritic cells. This method should be useful for regenerative medicine, disease-specific iPSC studies and drug discovery.
authors.affiliation: Department of Clinilcal Application, Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan.
authors.forename: Masakatsu D
authors.initials: MD
authors.lastname: Yanagimachi
authors.forename: Akira
authors.initials: A
authors.lastname: Niwa
authors.forename: Takayuki
authors.initials: T
authors.lastname: Tanaka
authors.forename: Fumiko
authors.initials: F
authors.lastname: Honda-Ozaki
authors.forename: Seiko
authors.initials: S
authors.lastname: Nishimoto
authors.forename: Yuuki
authors.initials: Y
authors.lastname: Murata
authors.forename: Takahiro
authors.initials: T
authors.lastname: Yasumi
authors.forename: Jun
authors.initials: J
authors.lastname: Ito
authors.forename: Shota
authors.initials: S
authors.lastname: Tomida
authors.forename: Koichi
authors.initials: K
authors.lastname: Oshima
authors.forename: Isao
authors.initials: I
authors.lastname: Asaka
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Goto
authors.forename: Toshio
authors.initials: T
authors.lastname: Heike
authors.forename: Tatsutoshi
authors.initials: T
authors.lastname: Nakahata
authors.forename: Megumu K
authors.initials: MK
authors.lastname: Saito
mesh_terms: D000943:Antigens, Differentiation / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000166:cytology / Q000378:metabolism*; D053595:Embryonic Stem Cells / Q000502:physiology*; D006801:Humans; D018189:Immunomagnetic Separation; D057026:Induced Pluripotent Stem Cells / Q000502:physiology*; D008264:Macrophages / Q000166:cytology / Q000378:metabolism*; D010641:Phenotype
chemical_list: D000943:Antigens, Differentiation; D016895:Culture Media, Serum-Free; D016207:Cytokines