title: Dye exclusion microfluidic microscopy.
abstract: We present an optical system to measure height maps of non-adherent cells as they flow through a microfluidic channel. The cells are suspended in an index-matching absorbing buffer, where cell height is evaluated by measuring the difference in absorption between the cell and the background. Unlike interferometric microscopes, the measured cell height is nearly independent of the cell's optical properties. The height maps are captured using a single exposure of a color camera, and consequently the system is capable of high-throughput characterization of large collections of cells. Using this system, we have measured more than 1600 height maps and volumes of three different leukemia cell lines.
authors.affiliation: Rowland Institute at Harvard, Harvard University, Cambridge, Massachusetts 02142, USA. schonbrun@rowland.harvard.edu
authors.forename: Ethan
authors.initials: E
authors.lastname: Schonbrun
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Di Caprio
authors.forename: Diane
authors.initials: D
authors.lastname: Schaak
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D058948:Cell Tracking / Q000295:instrumentation*; D003124:Colorimetry / Q000295:instrumentation*; D004396:Coloring Agents; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D007938:Leukemia / Q000473:pathology*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008853:Microscopy / Q000295:instrumentation*
chemical_list: D004396:Coloring Agents