title: N-acetyltransferase-2 genotypes among Jordanian patients with diabetes mellitus.
abstract: BACKGROUND
  There are inconsistent reports concerning N-acetyltransferase 2 (NAT2) genotypes in diabetes mellitus (DM).
  
  
  OBJECTIVE
  The objective of the study was to explore any association between NAT2 genotypes and Type 1 and Type 2 DM in Jordanians.
  
  
  METHODS
  106 Type 1 and 110 Type 2 DM patients attending the "National Center for Diabetes, Endocrinology and Genetics", Amman, Jordan, were included in the study. DNA was extracted from venous blood using a commercial DNA extraction kit. NAT2 genotyping was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
  
  
  RESULTS
  The frequency of the genotype that encodes rapid acetylation (the wild-type genotype NAT2*4/4) was similar in the two types of diabetes mellitus. Those which encode intermediate acetylation (NAT2*4/5, NAT2*4/6, and NAT2*4/7) were higher in Type 2 diabetes (0.482) compared to Type 1 diabetes (0.339), while the frequency of genotypes which encode slow acetylation (NAT2*5/5, NAT2*5/6, NAT2*5/7, NAT2*6/6, NAT2*6/7, and NAT2*7/7) were higher in Type 1 diabetes (0.547) compared to Type 2 diabetes (0.418).
  
  
  CONCLUSIONS
  There is excess of genotypes encoding intermediate acetylation in Type 2 DM and an excess of slow acetylator genotypes in Type 1 DM. Furthermore, NAT2*4/6 genotype (which encodes intermediate acetylation) was more prevalent in Type 2 DM. Type 1 DM behaved similar to non-diabetic controls in regard to acetylation status.
authors.affiliation: Department of Pharmacology, Faculty of Medicine, The University of Jordan, Amman 11942, Jordan. y.irshaid@ju.edu.jo
authors.forename: Yacoub M
authors.initials: YM
authors.lastname: Irshaid
authors.forename: Mousa A
authors.initials: MA
authors.lastname: Abujbara
authors.forename: Kamel M
authors.initials: KM
authors.lastname: Ajlouni
authors.forename: Mohammed
authors.initials: M
authors.lastname: El-Khateeb
authors.forename: Yazun B
authors.initials: YB
authors.lastname: Jarrar
mesh_terms: D000107:Acetylation; D000293:Adolescent; D000328:Adult; D000368:Aged; D018912:Arabs / Q000235:genetics*; D001191:Arylamine N-Acetyltransferase / Q000235:genetics* / Q000378:metabolism; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000201:enzymology / Q000208:ethnology / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000201:enzymology / Q000208:ethnology / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D007597:Jordan / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D010641:Phenotype; D017422:Sequence Analysis, DNA; D055815:Young Adult
chemical_list: D001191:Arylamine N-Acetyltransferase; C478900:NAT2 protein, human