title: Imaging of pharmacokinetic rates of indocyanine green in mouse liver with a hybrid fluorescence molecular tomography/x-ray computed tomography system.
abstract: Pharmacokinetic rates have the potential to provide quantitative physiological and pathological information for biological studies and drug development. Fluorescence molecular tomography (FMT) is an attractive imaging tool for three-dimensionally resolving fluorophore distribution in small animals. In this letter, pharmacokinetic rates of indocyanine green (ICG) in mouse liver are imaged with a hybrid FMT and x-ray computed tomography (XCT) system. A recently developed FMT method using structural priors from an XCT system is adopted to improve the quality of FMT reconstruction. In the in vivo experiments, images of uptake and excretion rates of ICG in mouse liver are obtained, which can be used to quantitatively evaluate liver function. The accuracy of the results is validated by a fiber-based fluorescence measurement system.
authors.affiliation: Tsinghua University, School of Medicine, Department of Biomedical Engineering, Beijing 100084, China.
authors.forename: Guanglei
authors.initials: G
authors.lastname: Zhang
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.forename: Yun
authors.initials: Y
authors.lastname: He
authors.forename: Jianwen
authors.initials: J
authors.lastname: Luo
authors.forename: Jing
authors.initials: J
authors.lastname: Bai
mesh_terms: D000818:Animals; D007091:Image Processing, Computer-Assisted; D007208:Indocyanine Green / Q000737:chemistry / Q000493:pharmacokinetics*; D008099:Liver / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D061848:Optical Imaging / Q000379:methods*; D014057:Tomography, X-Ray Computed / Q000379:methods*
chemical_list: D007208:Indocyanine Green