title: Cep57 protein is required for cytokinesis by facilitating central spindle microtubule organization.
abstract: Cytokinesis is the final stage of cell division in which the cytoplasm of a cell is divided into two daughter cells after the segregation of genetic material, and the central spindle and midbody are considered to be the essential structures required for the initiation and completion of cytokinesis. Here, we determined that the centrosome protein Cep57, which is localized to the central spindle and midbody, acts as a spindle organizer and is required for cytokinesis. Depletion of Cep57 disrupted microtubule assembly of the central spindle and further led to abnormal midbody localization of MKLP1, Plk1, and Aurora B, which resulted in cytokinesis failure and the formation of binuclear cells. Furthermore, we found that Cep57 directly recruited Tektin 1 to the midbody matrix to regulate microtubule organization. Thus, our data reveal that Cep57 is essential for cytokinesis via regulation of central spindle assembly and formation of the midbody.
authors.affiliation: Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State Key Laboratory of Biomembrane and Membrane Bioengineering, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Runsheng
authors.initials: R
authors.lastname: He
authors.affiliation: Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State Key Laboratory of Biomembrane and Membrane Bioengineering, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Qixi
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State Key Laboratory of Biomembrane and Membrane Bioengineering, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Haining
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State Key Laboratory of Biomembrane and Membrane Bioengineering, College of Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Huang
authors.affiliation: Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State Key Laboratory of Biomembrane and Membrane Bioengineering, College of Life Sciences, Peking University, Beijing 100871, China; Center for Quantitative Biology, Peking University, Beijing 100871, China. Electronic address: chenjg@pku.edu.cn.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Chen
authors.affiliation: Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State Key Laboratory of Biomembrane and Membrane Bioengineering, College of Life Sciences, Peking University, Beijing 100871, China. Electronic address: junlinteng@pku.edu.cn.
authors.forename: Junlin
authors.initials: J
authors.lastname: Teng
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000502:physiology*; D018797:Cell Cycle Proteins; D018385:Centrosome / Q000378:metabolism; D048749:Cytokinesis / Q000502:physiology*; D005786:Gene Expression Regulation; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D008868:Microtubule Proteins / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000502:physiology*; D008870:Microtubules / Q000378:metabolism*; D008938:Mitosis; D009687:Nuclear Proteins / Q000502:physiology*; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D008941:Spindle Apparatus / Q000378:metabolism*
chemical_list: C474610:CEP57 protein, human; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D008868:Microtubule Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; C482107:Tsp57 protein, mouse; C044103:tektins
keywords: Cell Cycle; Central Spindle; Centrosome; Cep57; Cytokinesis; Microtubules; Midbody; Mitosis; Mitotic Spindle; Tektin 1