title: Impact of bleaching on subcritical water- and Formosolv-pretreated tulip tree to enhance enzyme accessibility.
abstract: A novel method was developed for fractionating cellulose microfibrils from forest residue (tulip tree sawdust) to enhance cellulose digestibility, particularly at minimum enzyme loadings. This method involved three main stages: selective hemicellulose solubilization by subcritical water (SCW) pretreatment, delignification of the SCW-pretreated solids using the Formosolv process, and deformylation/bleaching of the cellulose pulp with alkaline hydrogen peroxide solution. This process produced nearly 98% white cellulose microfibrils with 23-fold higher conversion to glucose as compared to the raw substrate after 72 h of enzymatic hydrolysis. This study showed that cellulose swelling had the greatest effect on the enzymatic hydrolysis efficiency of delignified pulp obtained by the Formosolv process.
authors.affiliation: School of Chemical and Biological Engineering and Institute of Chemical Processes, Seoul National University, Gwanak-gu, Seoul, Republic of Korea.
authors.forename: Aye Aye
authors.initials: AA
authors.lastname: Myint
authors.forename: Dae Sung
authors.initials: DS
authors.lastname: Kim
authors.forename: Hun Wook
authors.initials: HW
authors.lastname: Lee
authors.forename: Junho
authors.initials: J
authors.lastname: Yoon
authors.forename: In-Gyu
authors.initials: IG
authors.lastname: Choi
authors.forename: Joon Weon
authors.initials: JW
authors.lastname: Choi
authors.forename: Youn-Woo
authors.initials: YW
authors.lastname: Lee
mesh_terms: D056804:Biofuels; D057886:Bleaching Agents; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D005591:Chemical Fractionation / Q000379:methods*; D006861:Hydrogen Peroxide; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry / Q000302:isolation & purification*; D031567:Liriodendron / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D014934:Wood / Q000737:chemistry*
chemical_list: D056804:Biofuels; D057886:Bleaching Agents; D011134:Polysaccharides; C007916:hemicellulose; D002482:Cellulose; D008031:Lignin; D006861:Hydrogen Peroxide; D002480:Cellulase
keywords: Cellulase accessibility; Cellulose microfibrils; Formosolv process; Hydrogen peroxide; Subcritical water pretreatment