title: Androgen receptor agonism promotes an osteogenic gene program in preadipocytes.
abstract: Androgens regulate body composition by interacting with the androgen receptor (AR) to control gene expression in a tissue-specific manner. To identify novel regulatory roles for AR in preadipocytes, we created a 3T3-L1 cell line stably expressing human AR. We found AR expression is required for androgen-mediated inhibition of 3T3-L1 adipogenesis. This inhibition is characterized by decreased lipid accumulation, reduced expression of adipogenic genes, and induction of genes associated with osteoblast differentiation. Collectively, our results suggest androgens promote an osteogenic gene program at the expense of adipocyte differentiation.
authors.affiliation: Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA. hartig@bcm.edu
authors.forename: Sean M
authors.initials: SM
authors.lastname: Hartig
authors.forename: Qin
authors.initials: Q
authors.lastname: Feng
authors.forename: Scott A
authors.initials: SA
authors.lastname: Ochsner
authors.forename: Rui
authors.initials: R
authors.lastname: Xiao
authors.forename: Neil J
authors.initials: NJ
authors.lastname: McKenna
authors.forename: Sean E
authors.initials: SE
authors.lastname: McGuire
authors.forename: Bin
authors.initials: B
authors.lastname: He
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D050156:Adipogenesis; D000728:Androgens / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D005786:Gene Expression Regulation; D006801:Humans; D016086:Lentivirus / Q000235:genetics / Q000378:metabolism; D015741:Metribolone / Q000494:pharmacology; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D010012:Osteogenesis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011944:Receptors, Androgen / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D045165:Testosterone Congeners / Q000494:pharmacology; D059467:Transcriptome; D019076:Transgenes
chemical_list: C410798:AR protein, human; D000728:Androgens; D015415:Biomarkers; D012333:RNA, Messenger; D011944:Receptors, Androgen; D045165:Testosterone Congeners; D015741:Metribolone