title: Sentinel lymph node imaging by a fluorescently labeled DNA tetrahedron.
abstract: Sentinel lymph nodes (SLNs) are the first lymph nodes which cancer cells reach after traveling through lymphatic vessels from the primary tumor. Evaluating the nodal status is crucial in accurate staging of human cancers and accordingly determines prognosis and the most appropriate treatment. The commonly used methods for SLN identification in clinics are based on employment of a colloid of radionuclide or injection of a small dye. Although these methods have certainly contributed to improve surgical practice, new imaging materials are still required to overcome drawbacks of the techniques such as inconvenience of handling radioactive materials and short retention time of small dyes in SLNs. Here, we prepare a fluorescence-labeled DNA tetrahedron and perform SLN imaging by using the DNA nanoconstruct. With a successful identification of SLNs by the DNA nanoconstruct, we suggest that DNA tetrahedron hold great promises for clinical applications.
authors.affiliation: The Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Seongbuk-gu, Seoul 136-791, Republic of Korea.
authors.forename: Kyoung-Ran
authors.initials: KR
authors.lastname: Kim
authors.forename: Yong-Deok
authors.initials: YD
authors.lastname: Lee
authors.forename: Taemin
authors.initials: T
authors.lastname: Lee
authors.forename: Byeong-Su
authors.initials: BS
authors.lastname: Kim
authors.forename: Sehoon
authors.initials: S
authors.lastname: Kim
authors.forename: Dae-Ro
authors.initials: DR
authors.lastname: Ahn
mesh_terms: D000818:Animals; D002232:Carbocyanines / Q000008:administration & dosage / Q000378:metabolism; D002460:Cell Line; D004247:DNA / Q000737:chemistry*; D003952:Diagnostic Imaging / Q000379:methods*; D004705:Endocytosis; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000378:metabolism*; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D008198:Lymph Nodes / Q000033:anatomy & histology*; D051379:Mice; D009690:Nucleic Acid Conformation; D013194:Staining and Labeling; D014054:Tomography
chemical_list: D002232:Carbocyanines; D005456:Fluorescent Dyes; C085321:cyanine dye 5; D004247:DNA