title: Analysis of transcutaneous antigenic protein delivery by a hydrogel patch formulation.
abstract: We have developed a hydrogel patch, which could promote antigen penetration through stratum corneum (SC), and have demonstrated its safety and efficacy in animals and humans. For the availability improvement of our system, it is important to develop a device, which enhances antigen penetration through SC more efficiently. In this study, we have tried to collect the basic information involved in transcutaneous antigen delivery by investigating the immune event induced by our system and examining the effect of physical property of antigens or patch component on antigen penetration. A hydrogel patch delivered antigens through SC into skin, and some of Langerhans cells captured antigens, activated, and migrated to regional lymph nodes. We also showed that protein distribution into SC was regulated by various complexly-intertwined factors of proteins but not one particular parameter. Additionally, glycerin as the patch component contributed to the formation of SC hydration by patch application, which might be one of the factors of acceleration of protein penetration. On the basis of the present information, we are planning to modify the patch composition and establish the antigen modification technology for improvement in the efficacy of transcutaneous immunization.
authors.affiliation: Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Matsuo
authors.affiliation: Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan.
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Ishii
authors.affiliation: Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan.
authors.forename: Yasuaki
authors.initials: Y
authors.lastname: Kawai
authors.affiliation: Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Saiba
authors.affiliation: CosMED Pharmaceutical Company Ltd., Minami-ku, Kyoto 601-8014, Japan.
authors.forename: Ying-Shu
authors.initials: YS
authors.lastname: Quan
authors.affiliation: CosMED Pharmaceutical Company Ltd., Minami-ku, Kyoto 601-8014, Japan.
authors.forename: Fumio
authors.initials: F
authors.lastname: Kamiyama
authors.affiliation: Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan.
authors.forename: Sachiko
authors.initials: S
authors.lastname: Hirobe
authors.affiliation: Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan. Electronic address: okada@phs.osaka-u.ac.jp.
authors.forename: Naoki
authors.initials: N
authors.lastname: Okada
authors.affiliation: Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan. Electronic address: nakagawa@phs.osaka-u.ac.jp.
authors.forename: Shinsaku
authors.initials: S
authors.lastname: Nakagawa
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D000941:Antigens / Q000008:administration & dosage* / Q000276:immunology / Q000378:metabolism; D005260:Female; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D007110:Immunity, Active; D051379:Mice; D008807:Mice, Inbred BALB C; D012867:Skin / Q000378:metabolism*; D057968:Transdermal Patch; D014611:Vaccination; D014612:Vaccines / Q000008:administration & dosage* / Q000276:immunology / Q000493:pharmacokinetics
chemical_list: D000941:Antigens; D014612:Vaccines; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate