title: Genome-wide methylation analysis of prostate tissues reveals global methylation patterns of prostate cancer.
abstract: Altered genome methylation is a hallmark of human malignancies. In this study, high-throughput analyses of concordant gene methylation and expression events were performed for 91 human prostate specimens, including prostate tumor (T), matched normal adjacent to tumor (AT), and organ donor (OD). Methylated DNA in genomic DNA was immunoprecipitated with anti-methylcytidine antibodies and detected by Affymetrix human whole genome SNP 6.0 chips. Among the methylated CpG islands, 11,481 islands were found located in the promoter and exon 1 regions of 9295 genes. Genes (7641) were methylated frequently across OD, AT, and T samples, whereas 239 genes were differentially methylated in only T and 785 genes in both AT and T but not OD. Genes with promoter methylation and concordantly suppressed expression were identified. Pathway analysis suggested that many of the methylated genes in T and AT are involved in cell growth and mitogenesis. Classification analysis of the differentially methylated genes in T or OD produced a specificity of 89.4% and a sensitivity of 85.7%. The T and AT groups, however, were only slightly separated by the prediction analysis, indicating a strong field effect. A gene methylation prediction model was shown to predict prostate cancer relapse with sensitivity of 80.0% and specificity of 85.0%. These results suggest methylation patterns useful in predicting clinical outcomes of prostate cancer.
authors.affiliation: Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Luo
authors.forename: Ying
authors.initials: Y
authors.lastname: Ding
authors.forename: Rui
authors.initials: R
authors.lastname: Chen
authors.forename: George
authors.initials: G
authors.lastname: Michalopoulos
authors.forename: Joel
authors.initials: J
authors.lastname: Nelson
authors.forename: George
authors.initials: G
authors.lastname: Tseng
authors.forename: Yan P
authors.initials: YP
authors.lastname: Yu
mesh_terms: D018899:CpG Islands; D019175:DNA Methylation; D004273:DNA, Neoplasm / Q000235:genetics; D052138:Genes, Neoplasm; D055106:Genome-Wide Association Study; D006801:Humans; D008297:Male; D011379:Prognosis; D011401:Promoter Regions, Genetic; D011471:Prostatic Neoplasms / Q000235:genetics*; D012008:Recurrence; D012680:Sensitivity and Specificity
chemical_list: D004273:DNA, Neoplasm