title: Antinociceptive activity of Stephanolepis hispidus skin aqueous extract depends partly on opioid system activation.
abstract: Stephanolepis hispidus is one of the most common filefish species in Brazil. Its skin is traditionally used as a complementary treatment for inflammatory disorders. However, there are very few studies on chemical and pharmacological properties using the skin of this fish. This study was undertaken in order to investigate the effect of aqueous crude extract of S. hispidus skin (SAE) in different nociception models. Here, we report that intraperitoneal administration of SAE inhibited the abdominal constrictions induced by acetic acid in mice. In addition to the effect seen in the abdominal constriction model, SAE was also able to inhibit the hyperalgesia induced by carrageenan and prostaglandin E2 (PGE2) in mice. This potent antinociceptive effect was observed in the hot plate model too, but not in tail-flick test. Naloxone, an opioid receptor antagonist, was able to block the antinociceptive effect of SAE in the abdominal constriction and hot plate models. In addition, SAE did not present cytotoxic or genotoxic effect in human peripheral blood cells. Our results suggest that aqueous crude extract from S. hispidus skin has antinociceptive activity in close relationship with the partial activation of opioid receptors in the nervous system. Moreover, aqueous crude extract from S. hispidus skin does not present toxicity and is therefore endowed with the potential for pharmacological control of pain.
authors.affiliation: Laboratory of Inflammation, IOC, Fiocruz, Rio de Janeiro-RJ, 21045-900, Brazil. vfrias@ioc.fiocruz.br
authors.forename: Vinicius
authors.initials: V
authors.lastname: Carvalho
authors.forename: Lohengrin
authors.initials: L
authors.lastname: Fernandes
authors.forename: Taline
authors.initials: T
authors.lastname: Conde
authors.forename: Helena
authors.initials: H
authors.lastname: Zamith
authors.forename: Ronald
authors.initials: R
authors.lastname: Silva
authors.forename: Andrea
authors.initials: A
authors.lastname: Surrage
authors.forename: Valber
authors.initials: V
authors.lastname: Frutuoso
authors.forename: Hugo
authors.initials: H
authors.lastname: Castro-Faria-Neto
authors.forename: Fabio
authors.initials: F
authors.lastname: Amendoeira
mesh_terms: D000700:Analgesics / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D000818:Animals; D001938:Brazil; D004195:Disease Models, Animal; D005399:Fishes; D006801:Humans; D007274:Injections, Intraperitoneal; D008297:Male; D051379:Mice; D009270:Naloxone / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D010146:Pain / Q000188:drug therapy; D051381:Rats; D017208:Rats, Wistar; D011957:Receptors, Opioid / Q000187:drug effects / Q000378:metabolism; D012867:Skin / Q000378:metabolism*; D014020:Tissue Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity
chemical_list: D000700:Analgesics; D009292:Narcotic Antagonists; D011957:Receptors, Opioid; D014020:Tissue Extracts; D009270:Naloxone