title: Identification of immunogenic determinants of the spike protein of SARS-like coronavirus.
abstract: Bat SARS-like coronavirus (SL-CoV) has a genome organization almost identical to that of SARS-CoV, but the N-terminus of the Spike (S) proteins, which interacts with host receptor and is a major target of neutralizing antibodies against CoVs, of the two viruses has only 63-64% sequence identity. Although there have been reports studying the overall immunogenicity of SSL, knowledge on the precise location of immunodominant determinants for SSL is still lacking. In this study, using a series of truncated expressed SSL fragments and SSL specific mouse sera, we identified two immunogenic determinants for SSL. Importantly, one of the two regions seems to be located in a region not shared by known immunogenic determinants of the SSARS. This finding will be of potential use in future monitoring of SL-CoV infection in bats and spillover animals and in development of more effective vaccine to cover broad protection against this new group of coronaviruses.
authors.affiliation: Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences (CAS), Wuhan, 430071, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhou
authors.forename: Zhenggang
authors.initials: Z
authors.lastname: Han
authors.forename: Lin-Fa
authors.initials: LF
authors.lastname: Wang
authors.forename: Zhengli
authors.initials: Z
authors.lastname: Shi
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D006801:Humans; D017720:Molecular Epidemiology; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D014765:Viral Vaccines / Q000276:immunology
chemical_list: D014759:Viral Envelope Proteins; D014765:Viral Vaccines