title: A new approach to establish a cell line with reduced risk of endogenous retroviruses.
abstract: Endogenous retroviruses (ERVs) are integrated as DNA proviruses in the genomes of all mammalian species. Several ERVs are replication-competent and produced as fully infectious viruses from host cell. Thus, live-attenuated vaccines and biological substances have been prepared using the cell lines which may produce ERV. Indeed, we recently reported that several commercial live-attenuated vaccines for pets were contaminated with the infectious feline endogenous retrovirus, RD-114. In this study, to establish a cell line for vaccine manufacture with reduced risk of ERVs, we generated a cell line stably expressing human tetherin (Teth-CRFK cells). The release of infectious ERV from Teth-CRFK cells was suppressed to undetectable levels, while the production of parvovirus in Teth-CRFK cells was similar to that in parental CRFK cells. These observations suggest that Teth-CRFK cells will be useful as a cell line for the manufacture of live-attenuated vaccines or biological substances with reduced risk of ERV.
authors.affiliation: Department of Emerging Infectious Diseases, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan.
authors.forename: Aiko
authors.initials: A
authors.lastname: Fukuma
authors.forename: Rokusuke
authors.initials: R
authors.lastname: Yoshikawa
authors.forename: Takayuki
authors.initials: T
authors.lastname: Miyazawa
authors.forename: Jiro
authors.initials: J
authors.lastname: Yasuda
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics* / Q000378:metabolism; D002415:Cats; D060846:Cell Engineering / Q000379:methods; D002460:Cell Line; D004340:Drug Contamination / Q000517:prevention & control*; D020077:Endogenous Retroviruses / Q000254:growth & development; D018703:Founder Effect; D058851:GPI-Linked Proteins / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007668:Kidney / Q000166:cytology / Q000821:virology; D017992:Parvovirus / Q000502:physiology*; D012306:Risk; D014162:Transfection; D019459:Urothelium / Q000166:cytology* / Q000821:virology; D014612:Vaccines / Q000096:biosynthesis / Q000276:immunology / Q000592:standards; D014613:Vaccines, Attenuated; D057074:Virus Release / Q000502:physiology*; D014779:Virus Replication; D000074010:Bone Marrow Stromal Antigen 2
chemical_list: D015703:Antigens, CD; D058851:GPI-Linked Proteins; D014612:Vaccines; D014613:Vaccines, Attenuated; C094572:BST2 protein, human; D000074010:Bone Marrow Stromal Antigen 2