title: A replication study for association of 53 single nucleotide polymorphisms in a scoliosis prognostic test with progression of adolescent idiopathic scoliosis in Japanese.
abstract: METHODS
  A genetic association study of single nucleotide polymorphisms (SNPs) previously reported to be associated with curve progression of adolescent idiopathic scoliosis (AIS).
  
  
  OBJECTIVE
  To determine whether the association of 53 SNPs with curve progression reported in white patients with AIS are replicated in Japanese patients with AIS.
  
  
  BACKGROUND
  Predicting curve progression is important in clinical practice of AIS. The progression of AIS is reported to be associated with a number of genes. Associations with 53 SNPs have been reported, and the SNPs are used for a progression test in white patients with AIS; however, there has been no replication study for their association.
  
  
  METHODS
  We recruited 2117 patients with AIS with 10° or more (Cobb angle) of scoliosis curves. They were divided into progression and nonprogression groups according to their Cobb angle. We defined the progression of the curve as Cobb angle more than 50° for skeletally mature subjects and more than 40° for immature patients, subjects. We defined the nonprogression of the curve as Cobb angle 50° or less only for skeletally mature subjects. Of the 2117 patients, 1714 patients with AIS were allocated to either the progression or nonprogression group. We evaluated the association of 53 SNPs with curve progression by comparing risk allele frequencies between the 2 groups.
  
  
  RESULTS
  We evaluated the progression (N = 600) and nonprogression (N = 1114) subjects. Their risk allele frequencies were not different significantly. We found no replication of the association on AIS curve progression in any of the SNPs.
  
  
  CONCLUSIONS
  The associations of the 53 SNPs with progression of AIS curve are not definite. Large-scale association studies based on appropriate criteria for progression would be necessary to identify SNPs associated with the curve progression.
  
  
  METHODS
  N/A.
authors.affiliation: Laboratory of Bone and Joint Diseases, Center for Integrative Medical Sciences, RIKEN, Tokyo, Japan.
authors.forename: Yoji
authors.initials: Y
authors.lastname: Ogura
authors.forename: Yohei
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Ikuyo
authors.initials: I
authors.lastname: Kou
authors.forename: Masahiro
authors.initials: M
authors.lastname: Nakajima
authors.forename: Katsuki
authors.initials: K
authors.lastname: Kono
authors.forename: Noriaki
authors.initials: N
authors.lastname: Kawakami
authors.forename: Koki
authors.initials: K
authors.lastname: Uno
authors.forename: Manabu
authors.initials: M
authors.lastname: Ito
authors.forename: Shohei
authors.initials: S
authors.lastname: Minami
authors.forename: Haruhisa
authors.initials: H
authors.lastname: Yanagida
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Taneichi
authors.forename: Ikuho
authors.initials: I
authors.lastname: Yonezawa
authors.forename: Taichi
authors.initials: T
authors.lastname: Tsuji
authors.forename: Teppei
authors.initials: T
authors.lastname: Suzuki
authors.forename: Hideki
authors.initials: H
authors.lastname: Sudo
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Kotani
authors.forename: Kota
authors.initials: K
authors.lastname: Watanabe
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Chiba
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Toyama
authors.forename: Morio
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Shiro
authors.initials: S
authors.lastname: Ikegawa
mesh_terms: D000293:Adolescent; D044466:Asian People / Q000235:genetics; D002648:Child; D018450:Disease Progression; D005787:Gene Frequency; D056726:Genetic Association Studies / Q000379:methods*; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D007564:Japan; D015810:Linkage Disequilibrium; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D011379:Prognosis; D012307:Risk Factors; D012600:Scoliosis / Q000208:ethnology / Q000235:genetics*