title: NAD pattern and NADH oxidase activity in pea (Pisum sativum L.) under cadmium toxicity.
abstract: Seeds of pea (Pisum sativum L.) were germinated for 5 days by soaking in distilled water or 5 mM cadmium chloride. Compared to the control, cadmium (Cd) caused a reduction in percent germination and embryo growth. Pyridine nucleotide coenzyme concentrations were determined in cotyledons and embryonic axis. Nicotinamide adenine dinucleotide (NADH) oxidase activity was examined. Cd treatment caused a restriction in levels of reduced coenzyme form in the mitochondria and the post-mitochondrial fraction of cotyledons, and embryonic axis. The oxidized coenzyme form has been accumulated by Cd-treated mitochondria of both tissues. It was also found that NADH oxidase activity was stimulated. The relationship between coenzyme levels, seed germination, pea growth, and Cd stress has been reported.
authors.affiliation: Bio-Physiologie Cellulaires, Faculté des Sciences de Bizerte, 7021 Zarzouna, Tunisie ; Unité Mixte de Recherches, 1136 Interaction arbres-microorganismes INRA-Université Henri-Poincaré, IFR110, Faculté des Sciences, BP 239, 54506 Vandoeuvre cedex, France.
authors.forename: Moêz
authors.initials: M
authors.lastname: Smiri
authors.forename: Abdelilah
authors.initials: A
authors.lastname: Chaoui
authors.forename: Nicolas
authors.initials: N
authors.lastname: Rouhier
authors.forename: Eric
authors.initials: E
authors.lastname: Gelhaye
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Jacquot
authors.forename: Ezzedine
authors.initials: E
authors.lastname: El Ferjani
keywords: Cadmium; Germination; NAD; Pea