title: Pulmonary fibrosis effect of ammonium perchlorate exposure in rabbit.
abstract: Ammonium perchlorate (AP) is a kind of inorganic chemical. To evaluate the potential pulmonary fibrosis caused by occupational exposure to this compound, 25 male rabbits were randomly allocated into 5 groups to receive AP or bleomycin or saline by intratracheal injection. All rabbits were sacrificed and lung tissues were removed to prepare hematoxylin and eosin (HE) staining for microscope observation and to perform electron microscopy examine. In microscope observation, AP-instilled lung tissue showed inflammatory infiltrates, alveolar collapse, subpleural thickening, and lymphocyte proliferation. Electron microscopy examination of lung tissue showed massive fibroblast accumulation, collagen fiber hyperplasia, and dense collagen deposition. The histopathological changes were considered as effective indicator linking to the development of pulmonary fibrosis. These results demonstrated that chronic instillation of AP can induce pulmonary fibrosis. And these results revealed that AP has pulmonary toxic effect.
authors.affiliation: Institute of Industrial Toxicology, Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, People's Republic of China.
authors.forename: Fenghong
authors.initials: F
authors.lastname: Wu
authors.forename: Hongxia
authors.initials: H
authors.lastname: Chen
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhou
authors.forename: Rao
authors.initials: R
authors.lastname: Zhang
authors.forename: Miaohong
authors.initials: M
authors.lastname: Ding
authors.forename: Qin
authors.initials: Q
authors.lastname: Liu
authors.forename: Kai-liang
authors.initials: KL
authors.lastname: Peng
mesh_terms: D000395:Air Pollutants, Occupational / Q000633:toxicity*; D000818:Animals; D001761:Bleomycin / Q000633:toxicity*; D003094:Collagen / Q000378:metabolism; D004195:Disease Models, Animal; D004801:Eosine Yellowish-(YS) / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects; D006416:Hematoxylin / Q000378:metabolism; D006801:Humans; D007442:Intubation, Intratracheal; D008168:Lung / Q000166:cytology / Q000187:drug effects*; D008297:Male; D046529:Microscopy, Electron, Transmission; D009784:Occupational Diseases / Q000139:chemically induced* / Q000473:pathology; D010472:Perchlorates / Q000633:toxicity*; D011658:Pulmonary Fibrosis / Q000139:chemically induced* / Q000473:pathology; D000644:Quaternary Ammonium Compounds / Q000633:toxicity*; D011817:Rabbits; D011897:Random Allocation
chemical_list: D000395:Air Pollutants, Occupational; D010472:Perchlorates; D000644:Quaternary Ammonium Compounds; D001761:Bleomycin; D003094:Collagen; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin; C053506:ammonium perchlorate