title: Th17 immunity in children with allergic asthma and rhinitis: a pharmacological approach.
abstract: Th17 cells and IL-17A play a role in the development and progression of allergic diseases. We analyzed the IL-17A levels in sputum supernatants (Ss), nasal wash (NW) and plasma (P) from Healthy Controls (HC) and children with Asthma/Rhinitis. We tested the expression of IL-17A, RORγ(t) and FOXP3 in peripheral blood T-lymphocytes from intermittent and mild-moderate asthma. The effect of Budesonide and Formoterol was tested "in vitro" on IL-17A, RORγ(t) and FOXP3 expression in cultured T-lymphocytes from mild-moderate asthma/persistent rhinitis patients, and on nasal and bronchial epithelial cells stimulated with NW and Ss from mild-moderate asthma/persistent rhinitis. Further, the effect of 12 weeks of treatment with Budesonide and Formoterol was tested "in vivo" in T-lymphocytes from mild-moderate asthma/persistent rhinitis patients. IL-17A was increased in Ss, NW and P from children with mild-moderate asthma compared with intermittent and HC. In cultured T-lymphocytes IL-17A and RORγ(t) expression were higher in mild-moderate asthma/persistent rhinitis than in mild-moderate asthma/intermittent rhinitis, while FOXP3 was reduced. Budesonide with Formoterol reduced IL-17A and RORγ(t), while increased FOXP3 in cultured T-lymphocytes from mild-moderate asthma/persistent rhinitis, and reduced the IL-8 release mediated by IL-17A present in NW and Ss from mild-moderate asthma/persistent rhinitis in nasal and bronchial epithelial cells. Finally, Budesonide with Formoterol reduced IL-17A levels in P and Ss, CD4(+)IL-17A(+)T-cells, in naïve children with mild-moderate asthma/persistent rhinitis after 12 weeks of treatment. Th17 mediated immunity may be involved in the airway disease of children with allergic asthma and allergic rhinitis. Budesonide with Formoterol might be a useful tool for its therapeutic control.
authors.affiliation: Unit Ex vivo/In Vitro Models To Study The Immunopathology and the Pharmacology of Airway Diseases, Institute of Biomedicine and Molecular Immunology, Italian National Research Council, Palermo, Italy.
authors.forename: Giusy Daniela
authors.initials: GD
authors.lastname: Albano
authors.forename: Caterina
authors.initials: C
authors.lastname: Di Sano
authors.forename: Anna
authors.initials: A
authors.lastname: Bonanno
authors.forename: Loredana
authors.initials: L
authors.lastname: Riccobono
authors.forename: Rosalia
authors.initials: R
authors.lastname: Gagliardo
authors.forename: Pascal
authors.initials: P
authors.lastname: Chanez
authors.forename: Mark
authors.initials: M
authors.lastname: Gjomarkaj
authors.forename: Angela Marina
authors.initials: AM
authors.lastname: Montalbano
authors.forename: Giulia
authors.initials: G
authors.lastname: Anzalone
authors.forename: Stefania
authors.initials: S
authors.lastname: La Grutta
authors.forename: Fabio Luigi Massimo
authors.initials: FL
authors.lastname: Ricciardolo
authors.forename: Mirella
authors.initials: M
authors.lastname: Profita
mesh_terms: D000280:Administration, Inhalation; D000293:Adolescent; D058666:Adrenergic beta-2 Receptor Agonists / Q000008:administration & dosage / Q000494:pharmacology; D018927:Anti-Asthmatic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D001249:Asthma / Q000188:drug therapy / Q000276:immunology* / Q000378:metabolism; D019819:Budesonide / Q000008:administration & dosage / Q000494:pharmacology*; D016022:Case-Control Studies; D002478:Cells, Cultured; D002648:Child; D004983:Ethanolamines / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D051858:Forkhead Transcription Factors / Q000378:metabolism; D000068759:Formoterol Fumarate; D006801:Humans; D020381:Interleukin-17 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D008297:Male; D017817:Nasal Lavage Fluid; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3 / Q000378:metabolism; D012221:Rhinitis, Allergic, Perennial / Q000188:drug therapy / Q000276:immunology* / Q000378:metabolism; D013183:Sputum / Q000378:metabolism; D058504:Th17 Cells / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*
chemical_list: D058666:Adrenergic beta-2 Receptor Agonists; D018927:Anti-Asthmatic Agents; D004983:Ethanolamines; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; C508717:IL17A protein, human; D020381:Interleukin-17; D016209:Interleukin-8; D057132:Nuclear Receptor Subfamily 1, Group F, Member 3; D019819:Budesonide; D000068759:Formoterol Fumarate