title: Measuring free thyroxine levels in neonatal heel-prick samples.
abstract: The Dutch neonatal screening scheme for Congenital Hypothyroidism (CH) is primarily based on the determination of thyroxine (T4) in filter paper blood spots. In the lowest 5% of T4 values, thyroxine binding globulin (TBG) is measured in order to be able to correct for occasional low TBG levels. However, because the commercial TBG kit has been withdrawn from the market, alternative strategies are needed to be explored including the assessment of free T4. We evaluated the Neonatal Free Thyroxine (fT4) enzyme immunoassay (EIA) kit of Bio-Rad. FT4 as measured in a daily run of random samples correlated with T4. We also observed a correlation between fT4 and T4, and between fT4 and T4/TBG ratio in blood spots with low T4 concentrations. The correlation between fT4 and T4 in blood spots of proven CH-patients was highly significant. ROC curves were constructed for the fT4 assay and the T4/TBG ratio based on 27 CH patients and 215 controls with a complete set of data. The curves of both assays seemed to be rather similar. We conclude that the validity of the fT4 and the T4/TBG-approach seems to be the same. A study with a larger sample size giving the same or even more favorable results for the fT4-approach is necessary before we will change the present CH protocol.
authors.affiliation: Neonatal Screening Laboratory Amsterdam, Laboratory of Endocrinology, Academic Medical Centre, Meibergdreef 9, 1105AZ Amsterdam, The Netherlands. a.boelen@amc.uva.nl
authors.forename: Anita
authors.initials: A
authors.lastname: Boelen
authors.forename: Marja
authors.initials: M
authors.lastname: van Veen
authors.forename: Paul H
authors.initials: PH
authors.lastname: Verkerk
authors.forename: Guido
authors.initials: G
authors.lastname: Diependaal
authors.forename: Gerard
authors.initials: G
authors.lastname: Loeber
authors.forename: Bert
authors.initials: B
authors.lastname: Elvers
authors.forename: Erik
authors.initials: E
authors.lastname: Endert
mesh_terms: D001774:Blood Chemical Analysis / Q000379:methods* / Q000592:standards; D003409:Congenital Hypothyroidism / Q000175:diagnosis*; D005260:Female; D006365:Heel; D006801:Humans; D007118:Immunoassay; D007231:Infant, Newborn; D015997:Neonatal Screening; D015203:Reproducibility of Results; D013974:Thyroxine / Q000097:blood*
chemical_list: D013974:Thyroxine