title: Preliminary study of efficacy of hyaluronic acid on caustic esophageal burns in an experimental rat model.
abstract: BACKGROUND
  The aim of this study was to investigate the effectiveness of hyaluronic acid on the prevention of esophageal damage and stricture formation after experimental caustic (alkaline) esophageal injury in rats.
  
  
  METHODS
  Twenty-one Wistar albino rats were randomly divided into three groups. A caustic esophageal burn was created following the Gehanno model: Group l (n=7) underwent operation, but no injury; Group 2 (n=7) was injured and left untreated; and Group 3 (n=7) was injured and treated with hyaluronic acid, first topically and then orally by gavage (2×0.3mL; 12.5mg/mL for 7days). The caustic esophageal burn was created by instilling 25% NaOH into the distal esophagus. All rats were euthanized on day 22 for evaluation. The efficacy of hyaluronic acid treatment was assessed histopathologically and biochemically via blood determination of the total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), and sulfhydryl group (SH) and lipid hydroperoxidase (LOOH) levels. Statistical analyses were performed.
  
  
  RESULTS
  Weight gain was significantly lower in Group 2 than in the other two groups (P<0.05). The mean stenosis index, histopathologic damage score, TAS, TOS, OSI, and SH and LOOH levels were higher in Group 2 than in the other two groups. The mean stenosis index, inflammation, TAS, SH and OSI in Group 2 were significantly different than those in the other two groups (P<0.05).
  
  
  CONCLUSIONS
  Hyaluronic acid treatment is effective in treating damage and preventing strictures after caustic esophageal burn in rats.
authors.affiliation: Faculty of Medicine, Department of Pediatric Surgery, Harran University, Sanliurfa, Turkey. cevikmuazzez@gmail.com
authors.forename: Muazez
authors.initials: M
authors.lastname: Cevik
authors.forename: Tuncer
authors.initials: T
authors.lastname: Demir
authors.forename: Cetin Ali
authors.initials: CA
authors.lastname: Karadag
authors.forename: Muzaffer Aydin
authors.initials: MA
authors.lastname: Ketani
authors.forename: Hakim
authors.initials: H
authors.lastname: Celik
authors.forename: Davut Sinan
authors.initials: DS
authors.lastname: Kaplan
authors.forename: Mehmet Emin
authors.initials: ME
authors.lastname: Boleken
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002057:Burns, Chemical / Q000188:drug therapy* / Q000601:surgery; D002424:Caustics; D004195:Disease Models, Animal; D004940:Esophageal Stenosis / Q000517:prevention & control; D004947:Esophagus / Q000293:injuries* / Q000601:surgery; D006820:Hyaluronic Acid / Q000494:pharmacology*; D008054:Lipid Peroxides / Q000097:blood; D018384:Oxidative Stress; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D018709:Statistics, Nonparametric; D013438:Sulfhydryl Compounds / Q000097:blood; D015430:Weight Gain
chemical_list: D000975:Antioxidants; D002424:Caustics; D008054:Lipid Peroxides; D013438:Sulfhydryl Compounds; D006820:Hyaluronic Acid