title: Analysis and comparison of oxygen consumption of HepG2 cells in a monolayer and three-dimensional high density cell culture by use of a matrigrid®.
abstract: By the use of a MatriGrid® we have established a three-dimensional high density cell culture. The MatriGrid® is a culture medium permeable, polymeric scaffold with 187 microcavities. In these cavities (300 μm diameter and 207 μm deep) the cells can growth three-dimensionally. For these experiments we measured the oxygen consumption of HepG2 cell cultures in order to optimize cultivation conditions. We measured and compared the oxygen consumption, growth rate and vitality under three different cultivation conditions: monolayer, three-dimensional static and three-dimensional actively perfused. The results show that the cells in a three-dimensional cell culture consume less oxygen as in a monolayer cell culture and that the actively perfused three-dimensional cell culture in the MatriGrid® has a similar growth rate and vitality as the monolayer culture.
authors.affiliation: Department of Nano-Biosystem Technology, Ilmenau University of Technology, Gustav-Kirchhoff-Straße 7, Ilmenau, Thuringia, Germany. frank.weise@tu-ilmenau.de
authors.forename: Frank
authors.initials: F
authors.lastname: Weise
authors.forename: Uta
authors.initials: U
authors.lastname: Fernekorn
authors.forename: Jörg
authors.initials: J
authors.lastname: Hampl
authors.forename: Maren
authors.initials: M
authors.lastname: Klett
authors.forename: Andreas
authors.initials: A
authors.lastname: Schober
mesh_terms: D019149:Bioreactors; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D003470:Culture Media / Q000737:chemistry* / Q000494:pharmacology; D004867:Equipment Design; D056945:Hep G2 Cells; D006801:Humans; D008954:Models, Biological; D010100:Oxygen / Q000032:analysis / Q000378:metabolism; D010101:Oxygen Consumption / Q000502:physiology*
chemical_list: D003470:Culture Media; D010100:Oxygen
keywords: hepatocyte; mathematical modeling; oxygen uptake rate; three-dimensional cell culture