title: [Effect of quercetin on the expression of TGF-beta1 in human embryonic lung fibroblasts activated by the silicotic alveolar macrophages].
abstract: OBJECTIVE
  To explore the effect s of quercetin (Que) intraperitoneal pulmonary fibrosis.
  
  
  METHODS
  The silicotic alveolar macrophages were collected by bronchoalveolar lavage andincubated in vitro in the DMEM medium containing SiO2 (50 microg/ml) and DMEM medium without SiO2 for 18h. Then the AM supernatant incubated for 18h was collected and with quercetins (10, 20 and 40 micromol/L) as stimulus supernatant. HELFs were isolated by organize paste block method, and incubated with stimulus supernatants. The proliferation in the HELF was detected with MTT method. The expressions of TGF-beta1 in the HELF are detected with the immunocytochemistry method.
  
  
  RESULTS
  (1) Three groups of Que could inhibit the proliferation of HELF, and more concentration, inhibition is stronger. Three groups of Que compared with SiO2 + AM group (0.620 +/- 0.096), Que20 group (0.406 +/- 0.070) and Que40 group (0.334 +/- 0.050) compared with AM group (0.490 +/- 0.064), differences were significant (P < 0.01). (2) Three groups of Que could reduce the expression of TGF-beta1 in HELF, and compared with SiO2 + AM group (0.542 +/- 0.095) differences were significant (P < 0.05). Three groups of Que compared with AM group (0.394 +/- 0.109) and blank control group (0.314 +/- 0. 066) differences were not significant.
  
  
  CONCLUSIONS
  Quercetin could prevent and treat pulmonary fibrosis stimulated by silicon dioxide.
authors.affiliation: Jitang College, Hebei United University, Tangshan 063000, China.
authors.forename: Haibing
authors.initials: H
authors.lastname: Peng
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Jianxing
authors.initials: J
authors.lastname: Wang
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
authors.forename: Xianhua
authors.initials: X
authors.lastname: Wang
mesh_terms: D018893:Bronchoalveolar Lavage; D018920:Coculture Techniques; D003470:Culture Media; D004622:Embryo, Mammalian; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D008168:Lung / Q000166:cytology; D016676:Macrophages, Alveolar / Q000473:pathology*; D011794:Quercetin / Q000494:pharmacology*; D012822:Silicon Dioxide / Q000633:toxicity*; D012829:Silicosis / Q000378:metabolism / Q000473:pathology; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
chemical_list: D003470:Culture Media; D053773:Transforming Growth Factor beta1; D012822:Silicon Dioxide; D011794:Quercetin