title: Study of regulatory effect of tropomyosin on actin-myosin interaction in skeletal muscle by in vitro motility assay.
abstract: The interaction between myosin and actin in striated muscle tissue is regulated by Ca2+ via thin filament regulatory proteins. Skeletal muscle possesses a whole pattern of myosin and tropomyosin isoforms. The regulatory effect of tropomyosin on actin-myosin interaction was investigated by measuring the sliding velocity of both actin and actin-tropomyosin filaments over fast and slow skeletal myosins using the in vitro motility assay. The actin-tropomyosin filaments were reconstructed with tropomyosin isoforms from striated muscle tissue. It was found that tropomyosins with different content of α-, β-, and γ-chains added to actin filaments affect the sliding velocity of filaments in different ways. On the other hand, the sliding velocity of filaments with the same content of α-, β-, and γ-chains depends on myosin isoforms of striated muscle. The reciprocal effects of myosin and tropomyosin on actin-myosin interaction in striated muscle may play a significant role in maintenance of effective work of striated muscle both during ontogenesis and under pathological conditions.
authors.affiliation: Institute of Immunology and Physiology, Ural Division of the Russian Academy of Sciences, ul. Pervomaiskaya 91, Ekaterinburg, Russia.
authors.forename: G V
authors.initials: GV
authors.lastname: Kopylova
authors.forename: D V
authors.initials: DV
authors.lastname: Shchepkin
authors.forename: L V
authors.initials: LV
authors.lastname: Nikitina
mesh_terms: D000199:Actins / Q000737:chemistry / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D018482:Muscle, Skeletal / Q000737:chemistry / Q000378:metabolism*; D009218:Myosins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D020033:Protein Isoforms / Q000378:metabolism; D011817:Rabbits; D014335:Tropomyosin / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000199:Actins; D020033:Protein Isoforms; D014335:Tropomyosin; D009218:Myosins