title: Protective effect of N-acetylcysteine against ischemia/reperfusion injury in rat urinary bladders.
abstract: Ischemia/reperfusion (I/R) injury represents an important cause of bladder contractile dysfunction. One of the major causes leading to this dysfunction is thought to be reactive oxygen species formation. In this study, we investigated the potential benefit of N-acetylcysteine (NAC), a potent antioxidant that neutralizes free radicals, in a rat model of urinary bladder injury. NAC treatment rescues the reduction of contractile response to I/R injury in a dose-dependent manner. In addition, all levels of reactive oxygen species, lipid peroxidation, and NADPH-stimulated superoxide production in the I/R operation+NAC (I/R+NAC) group also decreased compared with a marked increase in the I/R operation+saline (I/R+S) group. Moreover, an in situ fluorohistological approach also showed that NAC reduces the generation of intracellular superoxides enlarged by I/R injury. Together, our findings suggest that NAC has a protective effect against the I/R-induced bladder contractile dysfunction via radical scavenging property.
authors.affiliation: Department of Urology, School of Medicine, Chungnam National University Hospital, Daejeon, 301-721, Korea.
authors.forename: Ju-Hyun
authors.initials: JH
authors.lastname: Shin
authors.forename: Gun-Hwa
authors.initials: GH
authors.lastname: Kim
authors.forename: Ki-Hak
authors.initials: KH
authors.lastname: Song
authors.forename: Yong-Gil
authors.initials: YG
authors.lastname: Na
authors.forename: Chong-Koo
authors.initials: CK
authors.lastname: Sul
authors.forename: Jae-Sung
authors.initials: JS
authors.lastname: Lim
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D009119:Muscle Contraction; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D018384:Oxidative Stress; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D001743:Urinary Bladder / Q000187:drug effects* / Q000503:physiopathology
chemical_list: D000975:Antioxidants; D000111:Acetylcysteine
keywords: N-acetylcysteine (NAC); contractile response; ischemia and reperfusion (I/R) injury; oxidative stress; urinary bladder