title: High-throughput determination of phenolic compounds in virgin olive oil using dispersive liquid-liquid microextraction- capillary zone electrophoresis.
abstract: This article reports a simple methodology using dispersive liquid-liquid microextraction combined with CZE. It has been applied for the simultaneous determination of phenolic compounds such as caffeic, gallic, vanillic, syringic, cinnamic, p-coumaric acids and oleuropein, apigenin, luteolin, 3-hydroxytyrosol, and tyrosol, in virgin olive oil (VOO). The optimized extraction conditions for 20 g of VOO were: extractant solvent: 400 μL boric acid 30 mM at pH 9.5; dispersive solvent: 300 μL carbon tetrachloride; vortex: 8 min; centrifugation: 3 min. The composition of the BGE was optimized resulting in the selection of a solution made of 30 mM boric acid at pH 9.5. As a strategy for on-line preconcentration a stacking step was applied, injecting a plug of water before sample injection. The short extraction time, centrifugation and electrophoretic steps allow the selective determination of phenolic compounds in VOO with satisfactory LODs (0.004-0.251 mg/kg), recoveries (89.4-101.0%), and RSD (less than 7.44% in peak area and less than 0.69% in migration time), compatible with the concentration levels present in the samples.
authors.affiliation: Instituto de Biología Agrícola de Mendoza (IBAM-CONICET), Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, Mendoza, Argentina.
authors.forename: Romina Paula
authors.initials: RP
authors.lastname: Monasterio
authors.forename: María
authors.initials: M
authors.lastname: de Los Angeles Fernández
authors.forename: María Fernanda
authors.initials: MF
authors.lastname: Silva
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D057166:High-Throughput Screening Assays; D057230:Limit of Detection; D059627:Liquid Phase Microextraction / Q000379:methods*; D000069463:Olive Oil; D010636:Phenols / Q000032:analysis*; D010938:Plant Oils / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D000069463:Olive Oil; D010636:Phenols; D010938:Plant Oils