title: The role of anti-dengue virus NS-1 and anti-protein disulfide isomerase antibodies on platelet aggregation in secondary dengue infection.
abstract: OBJECTIVE
  to observe the correlation between anti-NS-1 and anti PDI antibodies against platelets function disorder on secondary dengue infection.
  
  
  METHODS
  50 patients with secondary DV infection according to WHO criteria were observed by a cross sectional study. Patient's blood was collected on day 3, 5 and 7 after fever onset. Platelets aggregation test was done to prove the possibility of platelets dysfunction. Anti-NS-1 and anti-PDI antibodies were determined by solid phase ELISA.
  
  
  RESULTS
  the inhibition of platelets aggregation was increased among day of observation. Means value of inhibition on day 3 is 46.6%, day 5 is 52.5% and day 7 is 56%. There is a significant difference (p<0.05) of inhibition of platelet aggregation value between days of observation. The antibodies against NS-1 DV and PDI were detected in all 50 sera with the positive rate of 90% develop NS-1 antibodies and 72% of PDI antibodies, on day 3 of symptoms. The highest OD of NS-1 antibodies is detected on the day 3 and decreased on day 7. The OD of PDI antibodies was increased on day 3 and still increasing on day 7. There is a significant correlation between anti NS-1 and PDI antibodies (r=0.386-0.490), while the differences of OD between observation days are not significant (p>0.05).
  
  
  CONCLUSIONS
  the kinetics profile of NS1 and PDI antibodies responses, which were detected by the third day of symptoms. Dengue patients' sera inhibited platelets aggregation. NS-1 antibodies and PDI antibodies might have a role on the platelets aggregation dysfunction; however, there is no correlation between them. It is possible that other mechanism involve in the inhibition of platelets aggregation.
authors.affiliation: Department of Internal Medicine, Faculty of Medicine, University of Indonesia - Cipto Mangunkusumo Hospital. Jl. Diponegoro no. 71, Jakarta Pusat 10430, Indonesia. andhika_rachman@yahoo.com
authors.forename: Andhika
authors.initials: A
authors.lastname: Rachman
authors.forename: A R
authors.initials: AR
authors.lastname: Harahap
authors.forename: R M
authors.initials: RM
authors.lastname: Widhyasih
mesh_terms: D000704:Analysis of Variance; D000914:Antibodies, Viral / Q000097:blood / Q000494:pharmacology*; D003430:Cross-Sectional Studies; D003715:Dengue / Q000276:immunology*; D003716:Dengue Virus / Q000276:immunology*; D006801:Humans; D010974:Platelet Aggregation / Q000187:drug effects*; D019704:Protein Disulfide-Isomerases / Q000276:immunology*; D013997:Time Factors; D017361:Viral Nonstructural Proteins / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D017361:Viral Nonstructural Proteins; D019704:Protein Disulfide-Isomerases