title: Ageing impairs the T cell response to dendritic cells.
abstract: Dendritic cells (DCs) are critical in priming adaptive T-cell responses, but the effects of ageing on interactions between DCs and T cells are unclear. This study investigated the influence of ageing on the maturation of and cytokine production by human blood-enriched DCs, and the impact on T cell responses in an allogeneic mixed leucocyte reaction (MLR). DCs from old subjects (65-75 y) produced significantly less TNF-α and IFN-γ than young subjects (20-30 y) in response to lipopolysaccharide (LPS), but expression of maturation markers and co-stimulatory molecules was preserved. In the MLR, DCs from older subjects induced significantly restricted proliferation of young T cells, activation of CD8+ T cells and expression of IL-12 and IFN-γ in T cells compared with young DCs. T cells from older subjects responded more weakly to DC stimulation compared with young T cells, regardless of whether the DCs were derived from young or older subjects. In conclusion, the capacity of DCs to induce T cell activation is significantly impaired by ageing.
authors.affiliation: Department of Food and Nutritional Sciences, The University of Reading, Whiteknights PO Box 226, Reading RG6 6AP, UK.
authors.forename: Jialu
authors.initials: J
authors.lastname: You
authors.forename: Honglin
authors.initials: H
authors.lastname: Dong
authors.forename: Elizabeth R
authors.initials: ER
authors.lastname: Mann
authors.forename: Stella C
authors.initials: SC
authors.lastname: Knight
authors.forename: Parveen
authors.initials: P
authors.lastname: Yaqoob
mesh_terms: D000328:Adult; D000368:Aged; D000375:Aging / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D018664:Interleukin-12 / Q000096:biosynthesis; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008070:Lipopolysaccharides; D008213:Lymphocyte Activation / Q000276:immunology*; D007959:Lymphocyte Culture Test, Mixed; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis; D055815:Young Adult
chemical_list: D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D018664:Interleukin-12; D007371:Interferon-gamma