title: Bioactive polyketides isolated from agar-supported fermentation of Phomopsis sp. CMU-LMA, taking advantage of the scale-up device, Platotex.
abstract: Phomopsis sp. CMU-LMA was cultivated on agar-supported fermentation (Ag-SF) using the scale-up prototype Platotex. In total nine compounds were isolated from the ethyl acetate extract of the culture. Among them, compounds LMA-P1, Sch-642305, DHTO and LMA-P2 had already been reported in our previous work on liquid state fermentation. The trihydroxybenzene lactone cytosporone D and dothiorelone A has been recently isolated from Phomopsis and Magnaporthe species. In addition, three compounds were isolated consisting in the reduced methoxy derivative of Sch-642305 (1), a hydroxylated derivative of LMA-P2 (2) and a linear ethyl ester polyketide (3) similar to the previously reported LMA-P3. Antimicrobial activity and inhibition of Escherichia coli DnaG primase were investigated. Cytosporone D inhibited the E. coli DnaG primase, a Gram-negative antimicrobial target, with an IC50 of 0.25 mM.
authors.affiliation: Centre de Recherche de Gif, Institut de Chimie des Substances Naturelles, ICSN, Centre National de la Recherche Scientifique, CNRS, Avenue de la Terrasse, 91198 Gif-sur-Yvette cedex, France.
authors.forename: Emilie
authors.initials: E
authors.lastname: Adelin
authors.forename: Marie-Thérèse
authors.initials: MT
authors.lastname: Martin
authors.forename: Sylvie
authors.initials: S
authors.lastname: Cortial
authors.forename: Pascal
authors.initials: P
authors.lastname: Retailleau
authors.forename: Saisamorn
authors.initials: S
authors.lastname: Lumyong
authors.forename: Jamal
authors.initials: J
authors.lastname: Ouazzani
mesh_terms: D000362:Agar / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001203:Ascomycota / Q000737:chemistry* / Q000378:metabolism; D019149:Bioreactors; D019915:DNA Primase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D005285:Fermentation; D008826:Microbial Sensitivity Tests; D008958:Models, Molecular; D015394:Molecular Structure; D061065:Polyketides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D013329:Structure-Activity Relationship
chemical_list: D000900:Anti-Bacterial Agents; D004791:Enzyme Inhibitors; D061065:Polyketides; D000362:Agar; D019915:DNA Primase
keywords: Antimicrobial; DnaG primase; Fungal secondary metabolites; Phomopsis; Polyketide; Sch-642305