title: Characterization of cytotoxic compound from mangrove derived fungi Irpex hydnoides VB4.
abstract: OBJECTIVE
  To investigate the cytotoxic activity of endophytic fungi isolated from mangrove fungi.
  
  
  METHODS
  In the present study the DNA was isolated and the ITS region of 5.8s rRNA was amplified using specific primers ITS 1 and ITS4 and sequence was determined using automated sequencers. Blast search sequence similarity was found against the existing non redundant nucleotide sequence database thus, identified as Aspergilus flavus, Hyporcaea lixii, Aspergillus niger, Eutorium amstelodami, Irpex hydnoides and Neurospora crassa. Among the seven isolates, one fungi Irpex hydnoides was selected for further studies. The fungi were grown in sabouraud broth for five days and filtrate were separated and subjected to ethyl acetate for further studies.
  
  
  RESULTS
  Nearly half (49.25%) of the extracts showed activity (IC50 of 125µg/mL). These values were within the cutoff point of the National Cancer Institute criteria for cytotoxicity (IC50<20 µg/mL) in the screening of crude plant extracts. The GC MS analysis revealed that the active principals might be Tetradecane (6.26%) with the RT 8.606.
  
  
  CONCLUSIONS
  It is clear from the present study that mangrove fungi with bioactive metabolites can be expected to provide high quality biological material for high throughout biochemical, anti cancer screening programmes. The results help us conclude that the potential of using metabolic engineering and post genomic approaches to isolate more novel bioactive compounds and to make their possible commercial application is not far off.
authors.affiliation: Department of Biotechnology, Sathyabama University, India.
authors.forename: B Valentin
authors.initials: BV
authors.lastname: Bhimba
authors.forename: D A Agnel Defora
authors.initials: DA
authors.lastname: Franco
authors.forename: Geena Mary
authors.initials: GM
authors.lastname: Jose
authors.forename: Jibi Merin
authors.initials: JM
authors.lastname: Mathew
authors.forename: Elsa Lycias
authors.initials: EL
authors.lastname: Joel
mesh_terms: D001487:Basidiomycota / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D001688:Biological Products / Q000737:chemistry / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects; D021903:DNA, Ribosomal Spacer; D008401:Gas Chromatography-Mass Spectrometry; D056945:Hep G2 Cells; D006801:Humans; D029648:Verbenaceae / Q000382:microbiology*
chemical_list: D001688:Biological Products; D021903:DNA, Ribosomal Spacer
keywords: Bioactive metabolite; Characterization; Cytotoxic activity; Cytotoxic compound; Endophytic fungi; GC MS; ITS sequencing; Irpex hynoides; Mangrove; Mangrove fungi