title: Reactor performance and microbial community dynamics during solid-state anaerobic digestion of corn stover at mesophilic and thermophilic conditions.
abstract: Reactor performance and microbial community dynamics were investigated during solid state anaerobic digestion (SS-AD) of corn stover at mesophilic and thermophilic conditions. Thermophilic SS-AD led to faster and greater reductions of cellulose and hemicelluloses during the first 12 days compared to mesophilic SS-AD. However, accumulation of volatile fatty acids (VFAs) was 5-fold higher at thermophilic than mesophilic temperatures, resulting in a large pH drop during days 6-12 in the thermophilic reactors. Culture-based enumeration revealed 10-50 times greater populations of cellulolytic and xylanolytic microbes during thermophilic SS-AD than mesophilic SS-AD. DGGE analysis of PCR amplified 16S rRNA genes showed dynamic shifts, especially during the thermophilic SS-AD, of bacterial and archaeal communities over the 38 days of SS-AD as a result of acclimation of the initial seed microbial consortia to the lignocellulosic feedstock. The findings of this study can guide future studies to improve efficiency and stability of SS-AD.
authors.affiliation: Department of Food, Agricultural and Biological Engineering, The Ohio State University, Ohio Agricultural Research and Development Center, 1680 Madison Ave., Wooster, OH 44691, USA.
authors.forename: Jian
authors.initials: J
authors.lastname: Shi
authors.forename: Zhongjiang
authors.initials: Z
authors.lastname: Wang
authors.forename: Jill A
authors.initials: JA
authors.lastname: Stiverson
authors.forename: Zhongtang
authors.initials: Z
authors.lastname: Yu
authors.forename: Yebo
authors.initials: Y
authors.lastname: Li
mesh_terms: D000085:Acetates / Q000378:metabolism; D000693:Anaerobiosis; D001105:Archaea / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D001419:Bacteria / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D056804:Biofuels / Q000382:microbiology; D019149:Bioreactors / Q000382:microbiology*; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D002482:Cellulose / Q000378:metabolism; D058645:Denaturing Gradient Gel Electrophoresis; D005232:Fatty Acids, Volatile / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008697:Methane / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism; D013696:Temperature; D014835:Volatilization; D014866:Waste Products / Q000032:analysis*; D014990:Xylans / Q000378:metabolism; D003313:Zea mays / Q000378:metabolism*
chemical_list: D000085:Acetates; D056804:Biofuels; D005232:Fatty Acids, Volatile; D011134:Polysaccharides; D014866:Waste Products; D014990:Xylans; C007916:hemicellulose; D002482:Cellulose; D008697:Methane