title: Digital photogrammetry and histomorphometric assessment of the effect of non-coherent light (light-emitting diode) therapy (λ640 ± 20 nm) on the repair of third-degree burns in rats.
abstract: Recent studies have demonstrated the efficacy of coherent light therapy from the red region of the electromagnetic spectrum on the tissue-healing process. This study analysed the effect of non-coherent light therapy (light-emitting diode-LED) with or without silver sulfadiazine (sulpha) on the healing process of third-degree burns. In this study, 72 rats with third-degree burns were randomly divided into six groups (n = 12): Gr1 (control), Gr2 (non-contact LED), Gr3 (contact LED), Gr4 (sulfadiazine), Gr5 (sulfadiazine + non-contact LED) and Gr6 (sulfadiazine + contact LED). The groups treated with LED therapy received treatment every 48 h (λ = 640 ± 20 nm, 110 mW, 16 J/cm(2); 41 s with contact and 680 s without contact). The digital photometric and histomorphometric analyses were conducted after the burn occurred. The combination of sulpha and LED (contact or non-contact) improved the healing of burn wounds. These results demonstrate that the combination of silver sulfadiazine with LED therapy (λ = 640 ± 20 nm, 4 J/cm(2), without contact) improves healing of third-degree burn wounds, significantly reduces the lesion area and increases the granulation tissue, increases the number of fibroblasts, promotes collagen synthesis and prevents burn infections by accelerating recovery.
authors.affiliation: Faculdade Integral Diferencial-FACID, Rua Veterinário Bugyja Brito, 1354-Bairro Horto Florestal, 64052-410, Teresina, Piauí, Brazil, silvanafisio7@yahoo.com.br.
authors.forename: Silvana Maria Véras
authors.initials: SM
authors.lastname: Neves
authors.forename: Renata Amadei
authors.initials: RA
authors.lastname: Nicolau
authors.forename: Antônio Luiz Martins Maia
authors.initials: AL
authors.lastname: Filho
authors.forename: Lianna Martha Soares
authors.initials: LM
authors.lastname: Mendes
authors.forename: Ana Maria
authors.initials: AM
authors.lastname: Veloso
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000627:therapeutic use; D002056:Burns / Q000188:drug therapy / Q000473:pathology / Q000628:therapy*; D003131:Combined Modality Therapy; D006097:Granulation Tissue / Q000473:pathology; D008297:Male; D018919:Neovascularization, Physiologic; D010780:Photogrammetry; D010789:Phototherapy / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D012837:Silver Sulfadiazine / Q000627:therapeutic use; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000891:Anti-Infective Agents, Local; D012837:Silver Sulfadiazine