title: Medicinal plant extract (Ankaferd Blood Stopper) application in deep tissue injuries in rats: histopathological investigation of the effect on regional and systemic tissues.
abstract: BACKGROUND
  This study was planned to evaluate both the histopathological changes under light microscope as well as the systemic organ effects following application of Ankaferd Blood Stopper® (ABS) (a mixture of five plant extracts) in an animal model of deep tissue hemorrhage.
  
  
  METHODS
  A total of 50 Wistar Albino rats were divided into five groups of 10 rats each. The rats underwent femoral vein puncture and were treated with ABS tampon, ABS spray, or Surgicel, and one group was left untreated. After two weeks, each group underwent partial tissue excision from the same femoral region as well as from the brain, heart, kidney, and liver.
  
  
  RESULTS
  The specimens from all groups were obtained from the femoral region after two weeks and evaluated under light microscope. The light microscope revealed no histopathological changes in neurovascular structures or in deep connective tissues in any of the groups.
  
  
  CONCLUSIONS
  ABS provided hemostasis and was observed to stop bleeding. There were no histopathological changes at the tissue level and no pathological effects in other organs tissues under light microscope, and the remote organ tissue remained clear.
authors.affiliation: Department of Emergency Medicine, Sütçü İmam University Faculty of Medicine, Kahramanmaraş, Turkey. okumus@hotmail.com
authors.forename: Mehmet
authors.initials: M
authors.lastname: Okumuş
authors.forename: Kasım Zafer
authors.initials: KZ
authors.lastname: Yüksel
authors.forename: Davut
authors.initials: D
authors.lastname: Özbağ
authors.forename: Harun
authors.initials: H
authors.lastname: Çıralık
authors.forename: Zeki
authors.initials: Z
authors.lastname: Yılmaz
authors.forename: Yakup
authors.initials: Y
authors.lastname: Gümüşalan
authors.forename: Vedat
authors.initials: V
authors.lastname: Bakan
authors.forename: Ali Murat
authors.initials: AM
authors.lastname: Kalender
mesh_terms: D000818:Animals; D005260:Female; D005263:Femoral Artery / Q000293:injuries; D006470:Hemorrhage / Q000628:therapy*; D006489:Hemostatic Techniques; D010936:Plant Extracts / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D013630:Tampons, Surgical
chemical_list: D010936:Plant Extracts; C539751:ankaferd blood stopper