title: Destruction of oral biofilms formed in situ on machined titanium (Ti) surfaces by cold atmospheric plasma.
abstract: The decontamination of implant surfaces represents the basic procedure in the management of peri-implant diseases, but it is still a challenge. The study aimed to evaluate the degradation of oral biofilms grown in situ on machined titanium (Ti) discs by cold atmospheric plasma (CAP). ~200 Ti discs were exposed to the oral cavities of five healthy human volunteers for 72 h. The resulting biofilms were divided randomly between the following treatments: CAP (which varied in mean power, treatment duration, and/or the gas mixture), and untreated and treated controls (diode laser, air-abrasion, chlorhexidine). The viability, quantity, and morphology of the biofilms were determined by live/dead staining, inoculation onto blood agar, quantification of the total protein content, and scanning electron microscopy. Exposure to CAP significantly reduced the viability and quantity of biofilms compared with the positive control treatments. The efficacy of treatment with CAP correlated with the treatment duration and plasma power. No single method achieved complete biofilm removal; however, CAP may provide an effective support to established decontamination techniques for treatment of peri-implant diseases.
authors.affiliation: Clinic of Operative Dentistry, Periodontology and Preventive Dentistry, Saarland University Medical Center, Homburg, Germany.
authors.forename: Ahmad Nour
authors.initials: AN
authors.lastname: Idlibi
authors.forename: Fuad
authors.initials: F
authors.lastname: Al-Marrawi
authors.forename: Matthias
authors.initials: M
authors.lastname: Hannig
authors.forename: Antje
authors.initials: A
authors.lastname: Lehmann
authors.forename: Andre
authors.initials: A
authors.lastname: Rueppell
authors.forename: Axel
authors.initials: A
authors.lastname: Schindler
authors.forename: Holger
authors.initials: H
authors.lastname: Jentsch
authors.forename: Stefan
authors.initials: S
authors.lastname: Rupf
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D001419:Bacteria / Q000254:growth & development; D018441:Biofilms / Q000187:drug effects*; D003666:Decontamination / Q000379:methods; D015921:Dental Implants / Q000382:microbiology*; D006801:Humans; D054023:Lasers, Semiconductor; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D008856:Microscopy, Fluorescence; D058626:Plasma Gases / Q000737:chemistry / Q000494:pharmacology*; D012680:Sensitivity and Specificity; D013997:Time Factors; D014025:Titanium / Q000737:chemistry*; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents; D015921:Dental Implants; D058626:Plasma Gases; D014025:Titanium