title: Two dimensional electrophoresis of the exo-proteome produced from community acquired methicillin resistant Staphylococcus aureus belonging to clonal complex 80.
abstract: Two-dimensional electrophoresis (2DE) combined with mass spectrometry was used to characterize the exo-proteome secreted by two strains (ER13 and ER21) representing community acquired methicillin resistant Staphylococcus aureus (CA-MRSA) belonging to clonal complex 80 (CC80). Common spots were detected between the 2 gels using the Progenesis SameSpots software. Two hundred and fifty-one and 312 spots from the exo-proteome of ER13 and ER21 were resolved, respectively. 2DE overlap comparison showed that 59 spots were shared. LC-MS/MS analysis identified 57 proteins from these spots comprising about 21% extracellular, 48% cytoplasmic, 2% cytoplasmic membrane, 2% cell wall, and 26% with unknown localization. The identified proteins were classified with respect to their Gene Ontology (GO) annotation as ∼24% virulence determinants and toxins, ∼17% involved in carbohydrate metabolism, ∼14% involved in environmental stress, and ∼12% associated with cell division. The identification of the enterotoxin B from the exo-products of both strains used in our study, as belonging to CC80 was interesting.
authors.affiliation: Department of Structural Pathology, Graduate School of Medical and Dental Sciences, Niigata University, Niigata, Japan. shymaa21@yahoo.com
authors.forename: Shymaa
authors.initials: S
authors.lastname: Enany
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yoshida
authors.forename: Sameh
authors.initials: S
authors.lastname: Magdeldin
authors.forename: Xu
authors.initials: X
authors.lastname: Bo
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Mohamed
authors.initials: M
authors.lastname: Enany
authors.forename: Tadashi
authors.initials: T
authors.lastname: Yamamoto
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D019295:Computational Biology; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000378:metabolism*; D020543:Proteome / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics; D053719:Tandem Mass Spectrometry; D057825:Two-Dimensional Difference Gel Electrophoresis
chemical_list: D001426:Bacterial Proteins; D020543:Proteome
keywords: 2DE; CA-MRSA; Exo-proteome; LC–MS/MS