title: Mass spectrometry based phospholipidomics of mammalian thymus and leukemia patients: implication for function of iNKT cells.
abstract: In previous studies phospholipids have been proved to be involved in biochemical, physiological, and pathological processes. As a special class of phospholipids, peroxisome-derived lipids (PDLs) have been proved to be potential ligands of invariant natural killer T (iNKT) cells in recent studies. Here, on the basis of phospholipidomics, we focused on the relative quantity of PDLs extracted from mammalian thymus or bone marrow using electrospray ionization mass spectrometry (MS). In phospholipid analysis, we identified 12 classes of phospholipids and accounted for their relative quantities by comparing their relative abundances in the MS(1) map. Our results show that PDLs are present in mammalian thymus as well as mouse spleen and liver. Interestingly, the relative quantity of PDLs extracted from human acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL) bone marrows is higher than that extracted from bone marrow of healthy donors. Our results may help to explain the close correlation between PDLs and iNKT cell function in thymus, spleen, liver, and especially in leukemia patients. We think that our phospholipidomics work may reveal a function of iNKT cells.
authors.affiliation: Laboratory of Cellular and Molecular Tumor Immunology, Institutes of Biology and Medical Sciences, Jiangsu Laboratory of Infection Immunity, Soochow University, 199 Ren-Ai Road, Suzhou 215123, China.
authors.forename: Xiukun
authors.initials: X
authors.lastname: Xu
authors.forename: Yunhui
authors.initials: Y
authors.lastname: Yu
authors.forename: Zheng
authors.initials: Z
authors.lastname: Wang
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhu
authors.forename: Yanping
authors.initials: Y
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Zhu
authors.forename: Zijun
authors.initials: Z
authors.lastname: Chen
authors.forename: Yun
authors.initials: Y
authors.lastname: He
authors.forename: Linling
authors.initials: L
authors.lastname: Ju
authors.forename: Yunsen
authors.initials: Y
authors.lastname: Li
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D007938:Leukemia / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods*; D055432:Metabolomics; D051379:Mice; D055611:Natural Killer T-Cells / Q000378:metabolism*; D010743:Phospholipids / Q000737:chemistry / Q000378:metabolism*; D013950:Thymus Gland / Q000378:metabolism*
chemical_list: D010743:Phospholipids