title: The first isolation and molecular characterization of camelpox virus in Ethiopia.
abstract: A cross-sectional study was conducted from November 2011 to April 2012 in Chifra district of Afar and in Jigjiga Zone of Somali Regional States of Ethiopia with the aims of assessing the epidemiology of camelpox and isolate and molecularly characterize the virus. The study included a questionnaire, active disease search and virus isolation and sequencing. A total of 24 (4.50%) and 12 (3.0%) camels in Afar and Jigjiga respectively were found clinically sick of camelpox during the study period. The questionnaire survey indicated that camelpox is the most common disease in the areas in which 125 (96%) of the respondents reported the frequent occurrence of camelpox in their herds especially during rainy season. The PCR result revealed 12 out of 17 tested samples were positive, of which seven of them collected from Jigjiga zone showed the characteristic PCR positive bands of 881 bp size fragments while five of the Afar samples gave two faint bands. Ethiopian isolates, specially isolated from Somali have very high identity with comparable sequences of CMLV M-96 from Kazakhstan and CMLV CMS from Iran. Out of the total of 780 bp analogous sequences, Ethiopian isolates differ only in two positions, while CMLV-Teheran differed at four nucleotide positions. The successfull isolation and molecular characterization of camelpox virus in Ethiopia, which could help for early diagnosis and control of the disease in the country.
authors.affiliation: National Veterinary Institute, P.O. Box 19, Debre-Zeit, Ethiopia. gelagayayelet1@gmail.com
authors.forename: Gelagay
authors.initials: G
authors.lastname: Ayelet
authors.forename: Shiferaw
authors.initials: S
authors.lastname: Jenberie
authors.forename: Alebachew
authors.initials: A
authors.lastname: Belay
authors.forename: Awol
authors.initials: A
authors.lastname: Mohammed
authors.forename: Bereket
authors.initials: B
authors.lastname: Mola
authors.forename: Yonas
authors.initials: Y
authors.lastname: Gizaw
authors.forename: Yibeltal
authors.initials: Y
authors.lastname: Muhie
authors.forename: Esayas
authors.initials: E
authors.lastname: Gelaye
authors.forename: Kassahun
authors.initials: K
authors.lastname: Asmare
authors.forename: Eystein
authors.initials: E
authors.lastname: Skjerve
mesh_terms: D000367:Age Factors; D000818:Animals; D002162:Camelus / Q000821:virology*; D003430:Cross-Sectional Studies; D005002:Ethiopia / Q000453:epidemiology; D005814:Genes, Viral; D018155:Orthopoxvirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D011213:Poxviridae Infections / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary*; D015995:Prevalence; D012307:Risk Factors; D012621:Seasons; D012720:Severity of Illness Index; D012874:Skin Diseases, Infectious / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology; D011795:Surveys and Questionnaires