title: Intracellular protein cyclization catalyzed by exogenously transduced Streptococcus pyogenes sortase A.
abstract: Streptococcus pyogenes sortase A (SpSrtA) was transduced into mammalian cells. Then SpSrtA-mediated intracellular circularization of a model protein was confirmed in a time and dose-dependent manner by Western blotting analysis. Direct transduction of SpSrtA is expected to be a strong tool for more conditional specific protein modification in mammalian cells.
authors.affiliation: Department of Bioengineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yamaguchi
authors.forename: Hidehiko
authors.initials: H
authors.lastname: Hirakawa
authors.forename: Teruyuki
authors.initials: T
authors.lastname: Nagamune
mesh_terms: D019881:Aminoacyltransferases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D055162:Biocatalysis; D015153:Blotting, Western; D002460:Cell Line; D003500:Cyclization; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D008322:Mammals; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D013297:Streptococcus pyogenes / Q000201:enzymology* / Q000235:genetics; D013997:Time Factors; D014161:Transduction, Genetic
chemical_list: D001426:Bacterial Proteins; D011506:Proteins; D019881:Aminoacyltransferases; C120542:sortase A; D003546:Cysteine Endopeptidases
keywords: Cyclization; Mammalian cell; Protein modification; Protein transduction; Sortase A