title: Therapeutic potential of a pyridoxal-based vanadium(IV) complex showing selective cytotoxicity for cancer versus healthy cells.
abstract: Vanadium compounds can exert anticancer effects, partly due to inhibition of tyrosine phosphatases. Here, we report the effect of N,N'-ethylenebis (pyridoxylideneiminato) vanadium (IV) complex (Pyr2 enV(IV)), that induced 93% and 57% of cell mortality in A375 (human melanoma) and A549 (human lung carcinoma) cells, respectively; the mortality was <24% in other cancer cell lines and in human normal epidermal keratinocytes, lung cells and peripheral blood mononuclear cells. The mechanism of Pyr2 enV(IV) effect relied on apoptosis induction; this was triggered by ROS increase, followed by mitochondrial membrane depolarization. Indeed, the addition of N-acetyl cysteine to cell cultures abated Pyr2 enV(IV)-induced apoptosis. These results disclose the pro-apoptotic activity of Pyr2 enV(IV) and its mechanism, relying on intracellular ROS increase.
authors.affiliation: Department of Chemistry and Biology, University of Salerno, via Ponte Don Melillo, Fisciano SA, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Strianese
authors.forename: Anna
authors.initials: A
authors.lastname: Basile
authors.forename: Antonio
authors.initials: A
authors.lastname: Mazzone
authors.forename: Silvana
authors.initials: S
authors.lastname: Morello
authors.forename: Maria Caterina
authors.initials: MC
authors.lastname: Turco
authors.forename: Claudio
authors.initials: C
authors.lastname: Pellecchia
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology; D020169:Caspases / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D020128:Inhibitory Concentration 50; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D009369:Neoplasms / Q000188:drug therapy* / Q000473:pathology*; D011730:Pyridoxal / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D017382:Reactive Oxygen Species / Q000378:metabolism; D014410:Tumor Stem Cell Assay; D014639:Vanadium / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D017382:Reactive Oxygen Species; D014639:Vanadium; D011730:Pyridoxal; D020169:Caspases; D000111:Acetylcysteine