title: Non-erythroid alpha spectrin prevents telomere dysfunction after DNA interstrand cross-link damage.
abstract: Telomere integrity is critical for telomere function and genomic stability. We previously demonstrated that non-erythroid α-spectrin (αIISp) is present in mammalian cell nuclei where it is important in repair of DNA interstrand cross-links (ICLs) and chromosome stability. We now demonstrate that αIISp is also important for telomere maintenance after ICL damage. It localizes to telomeres in S phase after ICL damage where it has enhanced association with TRF1 and TRF2 and is required for recruitment of the ICL repair protein, XPF, to damage-induced foci at telomeres. In telomerase-positive normal cells depleted of αIISp by siRNA or in Fanconi anemia, complementation group A (FA-A) cells, where αIISp levels are 35-40% of normal, ICL damage results in failure of XPF to localize to telomeres, markedly increased telomere dysfunction-induced foci, followed by catastrophic loss of telomeres. Restoration of αIISp levels to normal in FA-A cells corrects these deficiencies. Our studies demonstrate that αIISp is critical for repair of DNA ICLs at telomeres, likely by facilitating the recruitment of repair proteins similar, but not identical, to its proposed role in repair of DNA ICLs in genomic DNA and that this function in turn is critical for telomere maintenance after DNA ICL damage.
authors.affiliation: Department of Pathology and Laboratory Medicine, UMDNJ - New Jersey Medical School, 185 South Orange Avenue, Newark, NJ 07042, USA.
authors.forename: Pan
authors.initials: P
authors.lastname: Zhang
authors.forename: Utz
authors.initials: U
authors.lastname: Herbig
authors.forename: Frederick
authors.initials: F
authors.lastname: Coffman
authors.forename: Muriel W
authors.initials: MW
authors.lastname: Lambert
mesh_terms: D002460:Cell Line; D002869:Chromosome Aberrations; D003432:Cross-Linking Reagents / Q000633:toxicity; D004249:DNA Damage; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000032:analysis; D052236:Fanconi Anemia Complementation Group D2 Protein / Q000032:analysis; D006801:Humans; D016196:S Phase / Q000235:genetics; D013049:Spectrin / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000502:physiology*; D016615:Telomere / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D035321:Telomeric Repeat Binding Protein 1 / Q000378:metabolism; D035341:Telomeric Repeat Binding Protein 2 / Q000378:metabolism
chemical_list: D003432:Cross-Linking Reagents; D004268:DNA-Binding Proteins; D052236:Fanconi Anemia Complementation Group D2 Protein; D035321:Telomeric Repeat Binding Protein 1; D035341:Telomeric Repeat Binding Protein 2; C095888:xeroderma pigmentosum group F protein; D013049:Spectrin