title: Identification and characterization of a cellulose binding heptapeptide revealed by phage display.
abstract: Cellulose nanowhiskers (CNWs) were used in conjunction with phage display technology to identify polypeptides which bind the crystalline region of cellulose. A consensus peptide WHWTYYW was identified to efficiently bind the CNWs. The binding affinities of specific phage particles were assessed using biopanning assays and enzyme-linked immunosorbent assay (ELISA). The WHWTYYW peptide was synthesized and isothermal titration calorimetry (ITC) analysis showed that the peptide exhibited a binding constant of ∼10(5) M(-1) toward the crystalline CNWs. In order to understand how the affinity of this peptide differs for noncrystalline cellulose, binding properties were characterized using cello-oligosaccharides as substrates. Binding analysis was performed using UV spectroscopy and fluorescence quenching experiments. The specific molecular interactions of the WHWTYYW peptide with cellohexaose were examined using nuclear magnetic resonance (NMR). Interactions of this peptide with crystalline cellulose were also investigated using classical molecular modeling and quantum mechanical calculations of (13)C NMR chemical shifts. The NMR experiments and calculations indicate that the WHWTYYW peptide exhibits a bent structure when bound, allowing the Y5 amino acid to form a CH/π stacking interaction and H-bond with the glucose ring of cellulose.
authors.affiliation: Intercollege Graduate Degree Program in Plant Biology, VIT University , Vellore-632014, India.
authors.forename: Jing
authors.initials: J
authors.lastname: Guo
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Catchmark
authors.forename: Mohamed Naseer Ali
authors.initials: MN
authors.lastname: Mohamed
authors.forename: Alan James
authors.initials: AJ
authors.lastname: Benesi
authors.forename: Ming
authors.initials: M
authors.lastname: Tien
authors.forename: Teh-Hui
authors.initials: TH
authors.lastname: Kao
authors.forename: Heath D
authors.initials: HD
authors.lastname: Watts
authors.forename: James D
authors.initials: JD
authors.lastname: Kubicki
mesh_terms: D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000737:chemistry*; D002482:Cellulose / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D009842:Oligopeptides / Q000737:chemistry*; D011485:Protein Binding
chemical_list: D009842:Oligopeptides; D002482:Cellulose