title: Real-time polymerase chain reaction for detection of Strongyloides stercoralis in stool.
abstract: The use of real-time polymerase chain reaction (PCR) for detection of Strongyloides stercoralis in stool has recently been described. We compared five DNA extraction methods by using normal human stool spiked with Strongyloides ratti and tested by using a real-time PCR. The PowerSoil kit was found to be the best technique in terms of sensitivity and ease of use. The PCR detected DNA extracted from one spiked S. ratti larva diluted 10⁻². The PowerSoil kit was then used to extract DNA from 160 human survey samples. All culture positive specimens with a high and moderate larval load were identified by real-time PCR, but only 15% of specimens with low larval load were positive. Specificity was greater than 99%. The combination of the PowerSoil kit and real-time PCR reliably detected high to moderate larval numbers of S. stercoralis in stools but was less sensitive when the larval load was low.
authors.affiliation: Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research, Westmead Hospital, Westmead, New South Wales, Australia. yasmin.du@yahoo.com
authors.forename: Yasmin
authors.initials: Y
authors.lastname: Sultana
authors.forename: Neisha
authors.initials: N
authors.lastname: Jeoffreys
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Watts
authors.forename: Gwendolyn L
authors.initials: GL
authors.lastname: Gilbert
authors.forename: Rogan
authors.initials: R
authors.lastname: Lee
mesh_terms: D000818:Animals; D001459:Bangladesh; D018509:DNA, Helminth / Q000302:isolation & purification*; D004195:Disease Models, Animal; D005243:Feces / Q000469:parasitology*; D006801:Humans; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D017170:Strongyloides ratti / Q000302:isolation & purification*; D017171:Strongyloides stercoralis / Q000302:isolation & purification*; D013322:Strongyloidiasis / Q000175:diagnosis
chemical_list: D018509:DNA, Helminth