title: The effect of prolactin and estrogen cross-talk on prolidase- dependent signaling in MCF-7 cells.
abstract: Estrogen and prolactin play important role in mammary carcinogenesis. The present study was undertaken to evaluate the effect of prolactin and estrogen cross-talk on HIF-1 α level and expression of some HIF-1 α- dependent signaling proteins. Since up-regulation of prolidase activity inhibits HIF-1 α degradation, the enzyme was considered as an interface of estrogen/prolactin signaling. The experiments were performed on MCF-7 cells cultured with prolactin in the presence or absence of estradiol. It was found that in the presence of estradiol, prolactin inhibits prolidase activity and its down-stream signaling proteins: HIF-1α, mTOR, AKT and MAPK p-38, while in the absence of estradiol, an opposite effect was observed. These results suggest that prolactin/estrogen cross-talk exert beneficial effect on prolidase-dependent down regulation of HIF-1α. It suggests that dual action of prolactin and estrogen may be considered as a strategy in therapy of breast cancer.
authors.affiliation: Department of Medicinal Chemistry, Medical University of Bialystok, Bialystok, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Surazynski
authors.forename: W
authors.initials: W
authors.lastname: Miltyk
authors.forename: S
authors.initials: S
authors.lastname: Wolczynski
authors.forename: J
authors.initials: J
authors.lastname: Palka
mesh_terms: D045744:Cell Line, Tumor; D004150:Dipeptidases / Q000502:physiology*; D004958:Estradiol / Q000494:pharmacology*; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000032:analysis; D010766:Phosphorylation; D011388:Prolactin / Q000494:pharmacology*; D020239:Receptor Cross-Talk; D015398:Signal Transduction; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D004958:Estradiol; D011388:Prolactin; C546842:MTOR protein, human; D058570:TOR Serine-Threonine Kinases; D048051:p38 Mitogen-Activated Protein Kinases; D004150:Dipeptidases; C022449:proline dipeptidase