title: Klebsiella phage vB_KleM-RaK2 - a giant singleton virus of the family Myoviridae.
abstract: At 346 kbp in size, the genome of a jumbo bacteriophage vB_KleM-RaK2 (RaK2) is the largest Klebsiella infecting myovirus genome sequenced to date. In total, 272 out of 534 RaK2 ORFs lack detectable database homologues. Based on the similarity to biologically defined proteins and/or MS/MS analysis, 117 of RaK2 ORFs were given a functional annotation, including 28 RaK2 ORFs coding for structural proteins that have no reliable homologues to annotated structural proteins in other organisms. The electron micrographs revealed elaborate spike-like structures on the tail fibers of Rak2, suggesting that this phage is an atypical myovirus. While head and tail proteins of RaK2 are mostly myoviridae-related, the bioinformatics analysis indicate that tail fibers/spikes of this phage are formed from podovirus-like peptides predominantly. Overall, these results provide evidence that bacteriophage RaK2 differs profoundly from previously studied viruses of the Myoviridae family.
authors.affiliation: Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Vilnius University, Vilnius, Lithuania.
authors.forename: Eugenijus
authors.initials: E
authors.lastname: Simoliūnas
authors.forename: Laura
authors.initials: L
authors.lastname: Kaliniene
authors.forename: Lidija
authors.initials: L
authors.lastname: Truncaitė
authors.forename: Aurelija
authors.initials: A
authors.lastname: Zajančkauskaitė
authors.forename: Juozas
authors.initials: J
authors.lastname: Staniulis
authors.forename: Algirdas
authors.initials: A
authors.lastname: Kaupinis
authors.forename: Marija
authors.initials: M
authors.lastname: Ger
authors.forename: Mindaugas
authors.initials: M
authors.lastname: Valius
authors.forename: Rolandas
authors.initials: R
authors.lastname: Meškys
mesh_terms: D001433:Bacteriolysis; D023061:Gene Order; D016679:Genome, Viral; D054884:Host-Pathogen Interactions; D007709:Klebsiella / Q000821:virology*; D008969:Molecular Sequence Data; D017900:Myoviridae / Q000502:physiology* / Q000648:ultrastructure; D009711:Nucleotides / Q000378:metabolism; D016366:Open Reading Frames; D012343:RNA, Transfer / Q000378:metabolism; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D056189:Viral Tropism; D014771:Virion / Q000648:ultrastructure; D014779:Virus Replication
chemical_list: D009711:Nucleotides; D014764:Viral Proteins; D012343:RNA, Transfer