title: A local regulatory network around three NAC transcription factors in stress responses and senescence in Arabidopsis leaves.
abstract: A model is presented describing the gene regulatory network surrounding three similar NAC transcription factors that have roles in Arabidopsis leaf senescence and stress responses. ANAC019, ANAC055 and ANAC072 belong to the same clade of NAC domain genes and have overlapping expression patterns. A combination of promoter DNA/protein interactions identified using yeast 1-hybrid analysis and modelling using gene expression time course data has been applied to predict the regulatory network upstream of these genes. Similarities and divergence in regulation during a variety of stress responses are predicted by different combinations of upstream transcription factors binding and also by the modelling. Mutant analysis with potential upstream genes was used to test and confirm some of the predicted interactions. Gene expression analysis in mutants of ANAC019 and ANAC055 at different times during leaf senescence has revealed a distinctly different role for each of these genes. Yeast 1-hybrid analysis is shown to be a valuable tool that can distinguish clades of binding proteins and be used to test and quantify protein binding to predicted promoter motifs.
authors.affiliation: Warwick Systems Biology Centre, University of Warwick, Coventry, CV4 7AL, UK.
authors.forename: Richard
authors.initials: R
authors.lastname: Hickman
authors.affiliation: School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK.
authors.forename: Claire
authors.initials: C
authors.lastname: Hill
authors.affiliation: Warwick Systems Biology Centre, University of Warwick, Coventry, CV4 7AL, UK.
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Penfold
authors.affiliation: Warwick Systems Biology Centre, University of Warwick, Coventry, CV4 7AL, UK.|School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK.
authors.forename: Emily
authors.initials: E
authors.lastname: Breeze
authors.affiliation: School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK.
authors.forename: Laura
authors.initials: L
authors.lastname: Bowden
authors.affiliation: Warwick Systems Biology Centre, University of Warwick, Coventry, CV4 7AL, UK.
authors.forename: Jonathan D
authors.initials: JD
authors.lastname: Moore
authors.affiliation: School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK.
authors.forename: Peijun
authors.initials: P
authors.lastname: Zhang
authors.affiliation: School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK.
authors.forename: Alison
authors.initials: A
authors.lastname: Jackson
authors.affiliation: Molecular Organisation and Assembly of Cells Doctoral Training Centre, University of Warwick, Coventry, CV4 7AL, UK.
authors.forename: Emma
authors.initials: E
authors.lastname: Cooke
authors.affiliation: School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK.
authors.forename: Findlay
authors.initials: F
authors.lastname: Bewicke-Copley
authors.affiliation: School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK.
authors.forename: Andrew
authors.initials: A
authors.lastname: Mead
authors.affiliation: Warwick Systems Biology Centre, University of Warwick, Coventry, CV4 7AL, UK.|School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK.
authors.forename: Jim
authors.initials: J
authors.lastname: Beynon
authors.affiliation: Warwick Systems Biology Centre, University of Warwick, Coventry, CV4 7AL, UK.
authors.forename: David L
authors.initials: DL
authors.lastname: Wild
authors.affiliation: Warwick Systems Biology Centre, University of Warwick, Coventry, CV4 7AL, UK.|School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK.
authors.forename: Katherine J
authors.initials: KJ
authors.lastname: Denby
authors.affiliation: Warwick Systems Biology Centre, University of Warwick, Coventry, CV4 7AL, UK.
authors.forename: Sascha
authors.initials: S
authors.lastname: Ott
authors.affiliation: Warwick Systems Biology Centre, University of Warwick, Coventry, CV4 7AL, UK.|School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK.
authors.forename: Vicky
authors.initials: V
authors.lastname: Buchanan-Wollaston
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D020171:Botrytis / Q000502:physiology*; D016922:Cellular Senescence; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D053263:Gene Regulatory Networks; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D010935:Plant Diseases / Q000382:microbiology; D018515:Plant Leaves / Q000235:genetics / Q000502:physiology; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D029681:Arabidopsis Proteins; D014157:Transcription Factors; C493659:NAC019 protein, Arabidopsis; C493660:ANAC055 protein, Arabidopsis; C493661:ANAC072 protein, Arabidopsis