title: Self-assembled monolayers with different chemical group substrates for the study of MCF-7 breast cancer cell line behavior.
abstract: The interactions between cancer cells and the extracellular matrix (ECM) are important with respect to a number of cell behavoirs, yet remain unclear. In this study, self-assembled monolayers with different terminal chemical groups (hydroxyl (-OH), carboxyl (-COOH), animo (-NH2), mercapto (-SH), and methyl (-CH3)) were employed as substrates for the culture of MCF-7 cells to examine effects on cell behavior. Cell spreading was investigated by scanning electron microscopy, tallin expression by immunofluorescence, proliferation rate by counting cell numbers, cell cycle by flow cytometry, metabolism by high-performance liquid chromatography and cell migration by live cell imaging. Annexin V-FITC (fluorescein isothiocyanate) and JC-1 assays were performed to determine cell apoptosis and mitochondrial membrane potential, respectively. Our results demonstrate the varied behaviors of MCF-7 cells in response to different chemical groups. Specifically, NH2 and COOH terminal functional groups promote proliferation, the production of lactic acid and mobility of MCF-7 cells; SH and OH terminal groups enhance the expression and distribution of tallin but result in weak cell proliferation, metabolism, spreading and mobility. These results are meaningful for uncovering the interactions between the ECM and cancer cells; they are potentially useful for designing novel cancer treatment strategies.
authors.affiliation: Bio-X Center, School of Life Science and Technology, Harbin Institute of Technology, Harbin 150080, People's Republic of China.
authors.forename: Hongji
authors.initials: H
authors.lastname: Yan
authors.forename: Song
authors.initials: S
authors.lastname: Zhang
authors.forename: Jin
authors.initials: J
authors.lastname: He
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Yin
authors.forename: Xiumei
authors.initials: X
authors.lastname: Wang
authors.forename: Xiongbiao
authors.initials: X
authors.lastname: Chen
authors.forename: Fuzhai
authors.initials: F
authors.lastname: Cui
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
authors.forename: Yongzhan
authors.initials: Y
authors.lastname: Nie
authors.forename: Weiming
authors.initials: W
authors.lastname: Tian
mesh_terms: D017209:Apoptosis; D001672:Biocompatible Materials / Q000737:chemistry*; D001943:Breast Neoplasms / Q000473:pathology* / Q000503:physiopathology*; D002448:Cell Adhesion; D002453:Cell Cycle; D002465:Cell Movement; D049109:Cell Proliferation; D005109:Extracellular Matrix / Q000737:chemistry* / Q000502:physiology; D005260:Female; D006801:Humans; D019344:Lactic Acid / Q000096:biosynthesis; D061986:MCF-7 Cells; D008422:Materials Testing; D009361:Neoplasm Invasiveness; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D019344:Lactic Acid