title: Synthesis, self-assembling and gene delivery potential of a novel highly unsaturated, conjugated cationic phospholipid.
abstract: The synthesis and self-assembling properties of a model compound in a new class of cationic phospholipids with a highly unsaturated conjugated fatty acid are described. In addition, the potential of this new lipid as a nucleic acid carrier was evaluated through lipoplex formulations employing 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) as helper lipid with and without the polycationic peptide protamine, together with a plasmid DNA (pDNA). Lipoplexes composed of this novel unsaturated lipid exhibited pDNA binding and protection from DNase I degradation when formulated with protamine. The new cationic lipid revealed transfection efficiency comparable to the commercial reference 1,2-dimyristoyl-sn-glycero-3-ethylphophocholine (EPC) in Chinese hamster ovary-K1 (CHO-K1) cells and performed equally to the standard reference Lipofectamine 2000 when the formulation included protamine.
authors.affiliation: Department of Chemistry, Norwegian University of Science and Technology, 7491 Trondheim, Norway.
authors.forename: Christer L
authors.initials: CL
authors.lastname: Øpstad
authors.forename: Hans-Richard
authors.initials: HR
authors.lastname: Sliwka
authors.forename: Vassilia
authors.initials: V
authors.lastname: Partali
authors.forename: Arnljot
authors.initials: A
authors.lastname: Elgsaeter
authors.forename: Philip
authors.initials: P
authors.lastname: Leopold
authors.forename: Emile
authors.initials: E
authors.lastname: Jubeli
authors.forename: Nada Abdul
authors.initials: NA
authors.lastname: Khalique
authors.forename: Liji
authors.initials: L
authors.lastname: Raju
authors.forename: Michael D
authors.initials: MD
authors.lastname: Pungente
mesh_terms: D000818:Animals; D016466:CHO Cells; D002412:Cations / Q000138:chemical synthesis / Q000737:chemistry; D006224:Cricetinae; D003412:Cricetulus; D005227:Fatty Acids / Q000737:chemistry; D018014:Gene Transfer Techniques; D010743:Phospholipids / Q000138:chemical synthesis* / Q000737:chemistry*
chemical_list: D002412:Cations; D005227:Fatty Acids; D010743:Phospholipids