title: Gene-expression changes in cerium chloride-induced injury of mouse hippocampus.
abstract: Cerium is widely used in many aspects of modern society, including agriculture, industry and medicine. It has been demonstrated to enter the ecological environment, is then transferred to humans through food chains, and causes toxic actions in several organs including the brain of animals. However, the neurotoxic molecular mechanisms are not clearly understood. In this study, mice were exposed to 0.5, 1, and 2 mg/kg BW cerium chloride (CeCl(3)) for 90 consecutive days, and their learning and memory ability as well as hippocampal gene expression profile were investigated. Our findings suggested that exposure to CeCl(3) led to hippocampal lesions, apoptosis, oxidative stress and impairment of spatial recognition memory. Furthermore, microarray data showed marked alterations in the expression of 154 genes involved in learning and memory, immunity and inflammation, signal transduction, apoptosis and response to stress in the 2 mg/kg CeCl(3) exposed hippocampi. Specifically, the significant up-regulation of Axud1, Cdc37, and Ube2v1 caused severe apoptosis, and great suppression of Adcy8, Fos, and Slc5a7 expression led to impairment of mouse cognitive ability. Therefore, Axud1, Cdc37, Ube2v1, Adcy8, Fos, and Slc5a7 may be potential biomarkers of hippocampal toxicity caused by CeCl3 exposure.
authors.affiliation: Medical College of Soochow University, Suzhou, PR China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Cheng
authors.forename: Haiquan
authors.initials: H
authors.lastname: Zhao
authors.forename: Yuguan
authors.initials: Y
authors.lastname: Ze
authors.forename: Junju
authors.initials: J
authors.lastname: Su
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Sheng
authors.forename: Liyuan
authors.initials: L
authors.lastname: Zhu
authors.forename: Ning
authors.initials: N
authors.lastname: Guan
authors.forename: Suxin
authors.initials: S
authors.lastname: Gui
authors.forename: Xuezi
authors.initials: X
authors.lastname: Sang
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Zhao
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Sun
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Cheng
authors.forename: Renping
authors.initials: R
authors.lastname: Hu
authors.forename: Fashui
authors.initials: F
authors.lastname: Hong
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D002563:Cerium / Q000493:pharmacokinetics / Q000633:toxicity*; D004785:Environmental Pollutants / Q000493:pharmacokinetics / Q000633:toxicity*; D005786:Gene Expression Regulation; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D008568:Memory / Q000187:drug effects; D051379:Mice; D009043:Motor Activity / Q000187:drug effects; D020411:Oligonucleotide Array Sequence Analysis; D009929:Organ Size; D018384:Oxidative Stress; D060888:Real-Time Polymerase Chain Reaction; D021641:Recognition, Psychology / Q000187:drug effects; D059467:Transcriptome / Q000187:drug effects*
chemical_list: D004785:Environmental Pollutants; D002563:Cerium; C026690:cerous chloride