title: Development of peptide nucleic acid probes for detection of the HER2 oncogene.
abstract: Peptide nucleic acids (PNAs) have gained much interest as molecular recognition tools in biology, medicine and chemistry. This is due to high hybridization efficiency to complimentary oligonucleotides and stability of the duplexes with RNA or DNA. We have synthesized 15/16-mer PNA probes to detect the HER2 mRNA. The performance of these probes to detect the HER2 target was evaluated by fluorescence imaging and fluorescence bead assays. The PNA probes have sufficiently discriminated between the wild type HER2 target and the mutant target with single base mismatches. Furthermore, the probes exhibited excellent linear concentration dependence between 0.4 to 400 fmol for the target gene. The results demonstrate potential application of PNAs as diagnostic probes with high specificity for quantitative measurements of amplifications or over-expressions of oncogenes.
authors.affiliation: Pediatric Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America.
authors.forename: Belhu
authors.initials: B
authors.lastname: Metaferia
authors.forename: Jun S
authors.initials: JS
authors.lastname: Wei
authors.forename: Young K
authors.initials: YK
authors.lastname: Song
authors.forename: Jennifer
authors.initials: J
authors.lastname: Evangelista
authors.forename: Konrad
authors.initials: K
authors.lastname: Aschenbach
authors.forename: Peter
authors.initials: P
authors.lastname: Johansson
authors.forename: Xinyu
authors.initials: X
authors.lastname: Wen
authors.forename: Qingrong
authors.initials: Q
authors.lastname: Chen
authors.forename: Albert
authors.initials: A
authors.lastname: Lee
authors.forename: Heidi
authors.initials: H
authors.lastname: Hempel
authors.forename: Jinesh S
authors.initials: JS
authors.lastname: Gheeya
authors.forename: Stephanie
authors.initials: S
authors.lastname: Getty
authors.forename: Romel
authors.initials: R
authors.lastname: Gomez
authors.forename: Javed
authors.initials: J
authors.lastname: Khan
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D018734:Genes, erbB-2; D006801:Humans; D015335:Molecular Probes; D020135:Peptide Nucleic Acids / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013499:Surface Properties
chemical_list: D017931:DNA Primers; D015335:Molecular Probes; D020135:Peptide Nucleic Acids