title: Protective effect of hydroferrate fluid, MRN-100, against lethality and hematopoietic tissue damage in γ-radiated Nile tilapia, Oreochromis niloticus.
abstract: Hydroferrate fluid, MRN-100, an iron-based compound derived from bivalent and trivalent ferrates, is a potent antioxidant compound. Therefore, we examined the protective effect of MRN-100 against γ-radiation-induced lethality and damage to hematopoietic tissues in fish. A total of 216 Nile tilapia fish (Oreochromis niloticus) were randomly divided into four groups. Group 1 served as a control that was administered no radiation and no MRN-100 treatment. Group 2 was exposed only to γ-radiation (15 Gy). Groups 3 and 4 were pre-treated with MRN-100 at doses of either 1 ml/l or 3 ml/l in water for 1 week, and subsequently exposed to radiation while continuing to receive MRN-100 for 27 days. The survival rate was measured, and biochemical and histopathological analyses of hematopoietic tissues were performed for the different treatment groups at 1 and 4 weeks post-radiation. Exposure to radiation reduced the survival rate to 27.7%, while treatment with MRN-100 maintained the survival rate at 87.2%. In addition, fish exposed to γ-radiation for 1 week showed a significant decrease in the total number of white blood cells (WBCs) and red blood cells (RBCs) series. However, treatment with MRN-100 protected the total WBC count and the RBCs series when compared with irradiated fish. Furthermore, significant histological lesions were observed in the hepatopancreas, spleen and gills of irradiated fish. However, treatment with MRN-100 protected the histopathology of various organs. We conclude that MRN-100 is a radioprotective agent in fish and may be useful as an adjuvant treatment to counteract the adverse side effects associated with radiation exposure.
authors.affiliation: Departments of Otolaryngology, Charles Drew University of Medicine and Science, 1621 East, 120th Street, Los Angeles, California 90059.
authors.forename: Mamdooh
authors.initials: M
authors.lastname: Ghoneum
authors.forename: Heba Allah M
authors.initials: HA
authors.lastname: Elbaghdady
authors.forename: Abdallah A
authors.initials: AA
authors.lastname: El-Shebly
authors.forename: Deyu
authors.initials: D
authors.lastname: Pan
authors.forename: Edward
authors.initials: E
authors.lastname: Assanah
authors.forename: Greg
authors.initials: G
authors.lastname: Lawson
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage; D002140:California / Q000453:epidemiology; D023681:Cichlids; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006402:Hematologic Diseases / Q000401:mortality* / Q000517:prevention & control*; D007501:Iron / Q000008:administration & dosage*; D015995:Prevalence; D011832:Radiation Injuries / Q000401:mortality* / Q000473:pathology / Q000517:prevention & control*; D011836:Radiation Tolerance / Q000187:drug effects*; D011837:Radiation-Protective Agents / Q000008:administration & dosage; D012307:Risk Factors; D015996:Survival Rate; D016896:Treatment Outcome
chemical_list: D000975:Antioxidants; D011837:Radiation-Protective Agents; C017311:ferrate ion; D007501:Iron
keywords: Oreochromis niloticus; hydroferrate fluid; radiation; survival