title: Genome-wide transcriptome analysis between small-tail Han sheep and the Surabaya fur sheep using high-throughput RNA sequencing.
abstract: The small-tail Han sheep and the Surabaya fur sheep are two local breeds in north China, which are characterized by high-fecundity and low-prolificacy breed respectively. Significant genetic differences between these two breeds have provided increasing interests in the identification and utilization of major prolificacy genes in these sheep. High prolificacy is a complex trait, and it is difficult to comprehensively identify the candidate genes related to this trait using the single molecular biology technique. To understand the molecular mechanisms of fecundity and provide more information about high prolificacy candidate genes in high- and low-fecundity sheep, we explored the utility of next-generation sequencing technology in this work. A total of 1.8 Gb sequencing reads were obtained and resulted in more than 20 000 contigs that averaged ∼300 bp in length. Ten differentially expressed genes were further verified by quantitative real-time RT-PCR to confirm the reliability of RNA-seq results. Our work will provide a basis for the future research of the sheep reproduction.
authors.affiliation: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China. mxy32@sohu.com
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Miao
authors.forename: Qingmiao
authors.initials: Q
authors.lastname: Luo
mesh_terms: D000818:Animals; D017849:Animals, Inbred Strains; D002681:China; D019295:Computational Biology / Q000379:methods; D005260:Female; D005298:Fertility; D020869:Gene Expression Profiling / Q000662:veterinary; D005786:Gene Expression Regulation; D063990:Gene Ontology; D055106:Genome-Wide Association Study / Q000662:veterinary; D059014:High-Throughput Nucleotide Sequencing / Q000662:veterinary; D010053:Ovary / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D017423:Sequence Analysis, RNA / Q000662:veterinary; D034561:Sheep, Domestic / Q000235:genetics / Q000502:physiology*; D013045:Species Specificity; D059467:Transcriptome
chemical_list: D012333:RNA, Messenger