title: α(2A) adrenoceptor-mediated presynaptic inhibition of GABAergic transmission in rat tuberomammillary nucleus neurons.
abstract: Histaminergic neurons within the tuberomammillary nucleus (TMN) play an important role in the regulation of sleep-wakefulness. Here, we report the adrenergic modulation of GABAergic transmission in rat TMN histaminergic neurons using a conventional whole-cell patch clamp technique. Norepinephrine (NE) reversibly decreased the amplitude of action potential-dependent GABAergic inhibitory post-synaptic currents (IPSCs) and increased the paired pulse ratio. The NE-induced inhibition of GABAergic IPSCs was mimicked by clonidine, a selective α2 adrenoceptor agonist. However, cirazoline and isoproterenol, nonselective α1 and β adrenoceptor agonists, respectively, had no effect on GABAergic IPSCs. The NE-induced inhibition of GABAergic IPSCs was significantly blocked by BRL44408, a selective α2A adrenoceptor antagonist, but not imiloxan or JP1302, a selective α2B and α2C adrenoceptor antagonists. The extent of NE-induced inhibition of GABAergic IPSCs was inversely proportional to the extracellular Ca(2+) concentration. Pharmacological agents affecting the activities of adenylyl cyclase or G-protein-coupled inwardly rectifying K(+) channels did not affect the NE-induced inhibition of GABAergic IPSCs. However, NE had no effect on the frequency and amplitude of GABAergic miniature IPSCs. These results suggest that NE acts on presynaptic α2A adrenoceptor to inhibit action potential-dependent GABA release via the inhibition of Ca(2+) influx from the extracellular space to GABAergic nerve terminals, and that this α2A adrenoceptor-mediated modulation of GABAergic transmission may be involved in regulating the excitability of TMN histaminergic neurons.
authors.affiliation: Department of Pharmacology, School of Dentistry, Kyungpook National University, Daegu, Republic of Korea.
authors.forename: Michiko
authors.initials: M
authors.lastname: Nakamura
authors.forename: Kyungho
authors.initials: K
authors.lastname: Suk
authors.forename: Maan-Gee
authors.initials: MG
authors.lastname: Lee
authors.forename: Il-Sung
authors.initials: IS
authors.lastname: Jang
mesh_terms: D000322:Adrenergic Agonists / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism; D005260:Female; D006632:Histamine / Q000378:metabolism; D007026:Hypothalamic Area, Lateral / Q000166:cytology / Q000502:physiology*; D066298:In Vitro Techniques; D008297:Male; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D009638:Norepinephrine / Q000494:pharmacology; D018408:Patch-Clamp Techniques; D017729:Presynaptic Terminals / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D018341:Receptors, Adrenergic, alpha-2 / Q000502:physiology*; D017661:Receptors, Presynaptic / Q000502:physiology*; D009435:Synaptic Transmission; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D000322:Adrenergic Agonists; D018341:Receptors, Adrenergic, alpha-2; D017661:Receptors, Presynaptic; D005680:gamma-Aminobutyric Acid; D006632:Histamine; D002118:Calcium; D009638:Norepinephrine