title: [New understanding of dog's prostatic urethral re-epithelial underwent two-micron laser prostatic vaporesection].
abstract: OBJECTIVE
  To study the re-epithelialization of dog's prostate urethra after two-micron laser resection of the prostate (TmLRP) and the role of CK34 (CK34βE12)-positive cells in this process.
  
  
  METHODS
  TmLRP were performed in 15 elder male dogs and wound specimens harvested at Days 3, 7 and 14 respectively. Immunohistochemical staining was performed to determine the expression levels of CK34 and prostate specific antigen (PSA) in prostatic urethra urothelial cells.
  
  
  RESULTS
  There was no CK34-positive cells in surgical wounds after 3 days while a cluster of CK34-positive cells were found to cover surgical wounds after 7 days, and these cells attached to residual prostate tissues. At Day 14 post-operation, positive expression of CK34 was found in basal cells of newborn urothelium.
  
  
  CONCLUSIONS
  Prostate tissues are the important organizations for re-epithelialization of prostatic urethra after benign prostate hyperplasia surgery, and CK34-positive basal cells play an important role in this process.
authors.affiliation: Department of Urology, Guizhou Provincial People's Hospital, Guiyang 550002, China.
authors.forename: Guang-heng
authors.initials: GH
authors.lastname: Luo
authors.forename: Lei
authors.initials: L
authors.lastname: Luo
authors.forename: Xiu-shu
authors.initials: XS
authors.lastname: Yang
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Zhao-lin
authors.initials: ZL
authors.lastname: Sun
authors.forename: Shu-jie
authors.initials: SJ
authors.lastname: Xia
mesh_terms: D000818:Animals; D004285:Dogs; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism* / Q000502:physiology*; D007633:Keratins / Q000378:metabolism; D007834:Lasers; D008297:Male; D011184:Postoperative Period; D020728:Transurethral Resection of Prostate / Q000379:methods; D014521:Urethra / Q000166:cytology*
chemical_list: D007633:Keratins