title: Semi-solid-state fermentation: a promising alternative for neomycin production by the actinomycete Streptomyces fradiae.
abstract: The production of neomycin by the actinomycete Streptomyces fradiae, under semi-solid-state fermentation conditions was the main subject of this study. Two supports (nylon sponge and orange peelings) were tested in order to determine the most suitable one for the production of neomycin by the above-mentioned microorganism. Nylon sponge led to the highest neomycin production, reaching a maximum value of 13,903 μg/mL on the 10th day of cultivation. As a control, the same experiment was performed under submerged fermentation (SmF) conditions, without solid support. Here the production of neomycin by S. fradiae was about 55-fold lower (i.e. 250 μg/mL) than that obtained for SSF.
authors.affiliation: Department of Chemical Engineering, Rovira i Virgili University, Av. Països Catalans 26, 43007 Tarragona, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Machado
authors.forename: José A
authors.initials: JA
authors.lastname: Teixeira
authors.forename: Susana
authors.initials: S
authors.lastname: Rodríguez-Couto
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology; D001709:Biotechnology / Q000379:methods*; D052978:Disk Diffusion Antimicrobial Tests; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D009355:Neomycin / Q000378:metabolism* / Q000494:pharmacology; D013212:Staphylococcus epidermidis / Q000187:drug effects; D013302:Streptomyces / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D009355:Neomycin; D005947:Glucose