title: Chloride intracellular channel 1 is overexpression in hepatic tumor and correlates with a poor prognosis.
abstract: Chloride intracellular channel 1 (CLIC1) is expressed in many human tissues and has been reported to be involved in the regulation of cell cycle, cell proliferation, and differentiation. Its roles in human hepatic tumor, however, remain unclear. The aim of this study was to investigate the clinicopathological significance and expression pattern of CLIC1 in human primary hepatic tumors. We examined the expression pattern of CLIC1 mRNA and protein in hepatic tumors using real-time quantitative RT-PCR and Western blot, respectively. CLIC1 protein and mRNA levels were significantly higher in cancerous tissues compared with corresponding normal tissue. In 85 hepatic tumor tissues, CLIC1 was significantly higher in 69 cases (81.2%), as determined by immunohistochemical staining. Increased CLIC1 expression was correlated with tumor size (p = 0.021), distant metastasis (p = 0.025), pathological TNM (pTNM) stage (p = 0.023), and poor survival (25.11 ± 2.27 vs 45.29 ± 4.28 months, p = 0.001). Our data show that increased CLIC1 protein expression is associated with clinicopathological factors and a poor prognosis of hepatic tumors, and suggest that CLIC1 might represent a valuable prognostic marker for human hepatic tumors.
authors.affiliation: Department of Hepatobiliary Surgery, Zhongshan Hospital Xiamen University, Fujian Provincial Key Laboratory of Chronic Liver Disease and Hepatocellular Carcinoma (Xiamen University Affiliated Zhongshan Hospital), Xiamen, Fujian, China; Research Institute of Digestive Disease, Xiamen University, Xiamen, Fujian, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Zhang
authors.forename: Xiao-Min
authors.initials: XM
authors.lastname: Wang
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Yin
authors.forename: Wen-Xiu
authors.initials: WX
authors.lastname: Zhao
authors.forename: Jian-Yin
authors.initials: JY
authors.lastname: Zhou
authors.forename: Bi-Xin
authors.initials: BX
authors.lastname: Zhao
authors.forename: Ping-Guo
authors.initials: PG
authors.lastname: Liu
mesh_terms: D018118:Chloride Channels / Q000032:analysis / Q000235:genetics / Q000502:physiology*; D006801:Humans; D008099:Liver / Q000737:chemistry; D008113:Liver Neoplasms / Q000737:chemistry / Q000401:mortality* / Q000473:pathology; D008875:Middle Aged; D011379:Prognosis; D012333:RNA, Messenger / Q000032:analysis
chemical_list: C501966:CLIC1 protein, human; D018118:Chloride Channels; D012333:RNA, Messenger
keywords: Hepatic tumor; chloride intracellular channel 1; prognosis