title: Genetic diversity of bile salt hydrolases among human intestinal bifidobacteria.
abstract: This study analyzes the application of degenerative primers for the screening of bile salt hydrolase-encoding genes (bsh) in various intestinal bifidobacteria. In the first stage, the design and evaluation of the universal PCR primers for amplifying the partial coding sequence of bile salt hydrolase in bifidobacteria were performed. The amplified bsh gene fragments were sequenced and the obtained sequences were compared to the bsh genes present in GenBank. The determined results showed the utility of the designed PCR primers for the amplification of partial gene encoding bile salt hydrolase in different intestinal bifidobacteria. Moreover, sequence analysis revealed that bile salt hydrolase-encoding genes may be used as valuable molecular markers for phylogenetic studies and identification of even closely related members of the genus Bifidobacterium.
authors.affiliation: Department of Biotechnology, Human Nutrition and Food Commodities, University of Life Sciences in Lublin, 8 Skromna St., 20-704, Lublin, Poland. piotr.jarocki@wp.pl
authors.forename: Piotr
authors.initials: P
authors.lastname: Jarocki
authors.forename: Zdzisław
authors.initials: Z
authors.lastname: Targoński
mesh_terms: D000581:Amidohydrolases / Q000235:genetics*; D001644:Bifidobacterium / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D041981:Gastrointestinal Tract / Q000382:microbiology; D014644:Genetic Variation; D006801:Humans; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D000581:Amidohydrolases; C023154:choloylglycine hydrolase