title: Effect of different plant growth regulators on micro-tuber induction and plant regeneration of Pinellia ternate (Thunb) Briet.
abstract: An efficient micropropagation system for Pinellia ternate (Thunb) Briet, a traditional Chinese medicinal plant, has been developed. Petiole and lamina of P. ternate were used as explants and cultured on Murashige and Skoog (MS) medium containing different concentrations of different plant growth regulators. The results indicated that low concentration of 2,4-dicholorophenoxy acetic acid (2,4-D), indole-3-acetic acid (IAA) and α-naphthalene acetic acid (NAA) were suitable for micro-tuber induction, but callus induction rate increased with increasing concentrations of growth regulators. Tubers induction rates of petiole and leaf were (81.8 %-100 %) and (89.4 %-96.0 %) respectively, when 0.2 mg l(-1) 2, 4-dicholorophenoxy acetic acid, indole-3-acetic acid or α-naphthalene acetic acid were present in the medium. Tubers induction rates of petiole and leaf cultured on MS medium supplemented with 0.2-0.5 mg l(-1) 6-benzyl amino purine (6-BAP) were (94.1 %-100 %) and (96.0 %-100 %) respectively. When the concentration of 2,4-dicholorophenoxy acetic acid, α-naphthalene acetic acid and 6-benzyl amino purine was increased to 2.0 mg l(-1), callus induction rates of petiole and leaf were 100 % and 98.2 %, 91.0 % and 36.0 %, 62.3 % and 70.0 %, respectively. Different concentration of kinetin (KT) and zeatin (ZT) had no significant effect on micro-tuber induction of petiole. Most petioles showed polarity during the cultivation of explants, when supplemented with different concentrations of auxin or cytokinin in the MS medium.
authors.affiliation: College of Life and Environment Sciences, Minzu University of China, Beijing, 100081 People's Republic of China ; College of Life and Environment Sciences, Minzu University of China, Zhong Guan Cun South Street 27, Beijing, 100081 People's Republic of China.
authors.forename: Junli
authors.initials: J
authors.lastname: Wang
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
authors.forename: Jue
authors.initials: J
authors.lastname: Wang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lu
authors.forename: Xuan
authors.initials: X
authors.lastname: Xiao
authors.forename: Weizhen
authors.initials: W
authors.lastname: Gong
authors.forename: Jikai
authors.initials: J
authors.lastname: Liu
keywords: Micro-tuber; Pinellia ternate (Thunb) Briet; Plant growth regulator; Regeneration