title: Evaluating the repair of DNA derived from formalin-fixed paraffin-embedded tissues prior to genomic profiling by SNP-CGH analysis.
abstract: Pathology archives contain vast resources of clinical material in the form of formalin-fixed paraffin-embedded (FFPE) tissue samples. Owing to the methods of tissue fixation and storage, the integrity of DNA and RNA available from FFPE tissue is compromized, which means obtaining informative data regarding epigenetic, genomic, and expression alterations can be challenging. Here, we have investigated the utility of repairing damaged DNA derived from FFPE tumors prior to single-nucleotide polymorphism (SNP) arrays for whole-genome DNA copy number analysis. DNA was extracted from FFPE samples spanning five decades, involving tumor material obtained from surgical specimens and postmortems. Various aspects of the protocol were assessed, including the method of DNA extraction, the role of Quality Control quantitative PCR (qPCR) in predicting sample success, and the effect of DNA restoration on assay performance, data quality, and the prediction of copy number aberrations (CNAs). DNA that had undergone the repair process yielded higher SNP call rates, reduced log R ratio variance, and improved calling of CNAs compared with matched FFPE DNA not subjected to repair. Reproducible mapping of genomic break points and detection of focal CNAs representing high-level gains and homozygous deletions (HD) were possible, even on autopsy material obtained in 1974. For example, DNA amplifications at the ERBB2 and EGFR gene loci and a HD mapping to 13q14.2 were validated using immunohistochemistry, in situ hybridization, and qPCR. The power of SNP arrays lies in the detection of allele-specific aberrations; however, this aspect of the analysis remains challenging, particularly in the distinction between loss of heterozygosity (LOH) and copy neutral LOH. In summary, attempting to repair DNA that is damaged during fixation and storage may be a useful pretreatment step for genomic studies of large archival FFPE cohorts with long-term follow-up or for understanding rare cancer types, where fresh frozen material is scarce.
authors.affiliation: Cancer Genetics Laboratory, Queensland Institute of Medical Research, Herston QLD, Australia.
authors.forename: Abdel Nasser
authors.initials: AN
authors.lastname: Hosein
authors.forename: Sarah
authors.initials: S
authors.lastname: Song
authors.forename: Amy E
authors.initials: AE
authors.lastname: McCart Reed
authors.forename: Janani
authors.initials: J
authors.lastname: Jayanthan
authors.forename: Lynne E
authors.initials: LE
authors.lastname: Reid
authors.forename: Jamie R
authors.initials: JR
authors.lastname: Kutasovic
authors.forename: Margaret C
authors.initials: MC
authors.lastname: Cummings
authors.forename: Nic
authors.initials: N
authors.lastname: Waddell
authors.forename: Sunil R
authors.initials: SR
authors.lastname: Lakhani
authors.forename: Georgia
authors.initials: G
authors.lastname: Chenevix-Trench
authors.forename: Peter T
authors.initials: PT
authors.lastname: Simpson
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics*; D055028:Comparative Genomic Hybridization; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D056915:DNA Copy Number Variations; D005260:Female; D005404:Fixatives; D005557:Formaldehyde; D006801:Humans; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D012107:Research Design; D014015:Tissue Banks
chemical_list: D005404:Fixatives; D005557:Formaldehyde; D004247:DNA