title: [Effect of recombinant human S100A8 on the proliferation and migration of periodontal ligament cells].
abstract: OBJECTIVE
  To investigate the effect of S100A8 on the proliferation and migration of periodontal ligament cells (PDLCs), and to learn the role of S100A8 in the development of periodontitis.
  
  
  METHODS
  PDLCs were treated with S100A8 in vitro before MTT and flow cytometry assays were performed. Transwell assay and wound assay were conducted to test the migratory activity of the PDLCs as well.
  
  
  RESULTS
  In the study, 10⁻⁷-10⁻⁵ mol/L recombined human S100A8 suppressed the proliferation of the PDLCs, while their proliferation was significantly inhibited with 10⁻⁵ mol/L S100A8 treatment for 48 h. And 10⁻⁹-10⁻⁷ mol/L S100A8 enhanced the migratory activity of the PDLCs while the effect of 10⁻⁹ mol/L S100A8 was statistically significant.
  
  
  CONCLUSIONS
  Increased level of S100A8 in periodontitis could lead to the inhibition of cell proliferation and apoptosis of PDLCs, but S100A8 could promote the migration of PDLCs when its concentration decreased after treatment.
authors.affiliation: Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing 100081, China.
authors.forename: Yun-fei
authors.initials: YF
authors.lastname: Zheng
authors.forename: Lei
authors.initials: L
authors.lastname: Peng
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Jian-xia
authors.initials: JX
authors.lastname: Hou
authors.forename: Huan-xin
authors.initials: HX
authors.lastname: Meng
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D040501:Calgranulin A / Q000502:physiology*; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D010513:Periodontal Ligament / Q000166:cytology* / Q000187:drug effects*; D061251:Primary Cell Culture; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D040501:Calgranulin A; D011994:Recombinant Proteins