title: Expression of adiponectin, leptin, and their receptors in ovarian endometrioma.
abstract: OBJECTIVE
  To evaluate the expression of leptin, leptin receptor (ObR), adiponectin, and adiponectin receptor (AdipoR) in ovarian endometriomas compared with normal endometrium, and to analyze relationships among adipokines and their receptors.
  
  
  METHODS
  Experimental study.
  
  
  METHODS
  A clinic for the treatment of endometriosis and basic research laboratories.
  
  
  METHODS
  Forty-four women with endometriosis and 42 age-matched women with no laparoscopic evidence of endometriosis as control subjects.
  
  
  METHODS
  Endometrial tissue samples were obtained during laparoscopic surgery.
  
  
  METHODS
  Immunohistochemical staining for leptin, ObR, adiponectin, and AdipoR was performed with the use of tissue microarray. Clinical characteristics were reviewed from the patient's medical records. The effect of leptin on the expression of adiponectin was evaluated in endometriotic cell line using real-time reverse-transcription polymerase chain reaction.
  
  
  RESULTS
  Positive expression rates of leptin and ObR were significantly higher in ovarian endometrioma compared with normal endometrium, but those of adiponectin and AdipoR were similar (ovarian endometrioma vs. normal endometrium, respectively: leptin 100% vs. 59.5%; ObR 72.7% vs. 33.3%; adiponectin 31.8% vs. 42.9%; AdipoR 88.6% vs. 73.8%). Expression of adipokines and their receptors did not show any correlation with disease stage. A positive correlation was found between expression of ObR and adiponectin (correlation coefficient 0.488; P=.001). Leptin treatment in endometriotic cells induced mRNA expression of adiponectin.
  
  
  CONCLUSIONS
  These data suggest that leptin and its receptor are induced in ovarian endometriomas, and that the leptin/ObR system regulates adiponectin gene expression in endometriotic cells.
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, Catholic University of Daegu, Daegu, South Korea.
authors.forename: Youn Seok
authors.initials: YS
authors.lastname: Choi
authors.forename: Hoon Kyu
authors.initials: HK
authors.lastname: Oh
authors.forename: Jung-Hye
authors.initials: JH
authors.lastname: Choi
mesh_terms: D052242:Adiponectin / Q000096:biosynthesis*; D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D002461:Cell Line, Transformed; D004715:Endometriosis / Q000378:metabolism* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020738:Leptin / Q000096:biosynthesis*; D010053:Ovary / Q000378:metabolism* / Q000473:pathology; D054419:Receptors, Adiponectin / Q000096:biosynthesis*; D054411:Receptors, Leptin / Q000096:biosynthesis*
chemical_list: C499918:ADIPOQ protein, human; D052242:Adiponectin; D015415:Biomarkers; C515448:LEPR protein, human; D020738:Leptin; D054419:Receptors, Adiponectin; D054411:Receptors, Leptin