title: Quantitative analysis of morphine in dried blood spots by using morphine-d3 pre-impregnated dried blood spot cards.
abstract: Two different internal standard dried blood spot (DBS) pre-impregnation procedures (prior to blood spotting) were investigated. In the first procedure DBS pre-impregnation is performed by immersing the DBS card fully into an internal standard solution. In the second procedure pre-impregnation is performed by pipetting a certain volume of an internal standard solution onto the DBS card. Morphine-d3 was used as the model compound for all experiments. The pre-impregnation procedure by immersing was further investigated with respect to homogeneity of impregnation, influence of different blood spotting techniques and the influence of spotting different blood volumes on the internal standard distribution, calibration and stability of pre-impregnated cards. Finally, the immersing procedure was used for the analysis of morphine in dried blood spots and the results were compared to the conventional procedure in which the internal standard morphine-d3 was added to the extraction solvent. The new pre-impregnated cards couple simplicity of operation and convenient use in the field to results equivalent to the conventional procedure.
authors.affiliation: DSM Resolve, P.O. Box 18, 6160 MD Geleen, The Netherlands. john.mommers@dsm.com
authors.forename: John
authors.initials: J
authors.lastname: Mommers
authors.forename: Ynze
authors.initials: Y
authors.lastname: Mengerink
authors.forename: Erik
authors.initials: E
authors.lastname: Ritzen
authors.forename: Jos
authors.initials: J
authors.lastname: Weusten
authors.forename: Jac
authors.initials: J
authors.lastname: van der Heijden
authors.forename: Sjoerd
authors.initials: S
authors.lastname: van der Wal
mesh_terms: D002138:Calibration; D059788:Dried Blood Spot Testing / Q000295:instrumentation* / Q000592:standards; D006801:Humans; D009020:Morphine / Q000097:blood*; D012015:Reference Standards; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry
chemical_list: D009020:Morphine