title: Identification of mutations involved in the requirement of potassium for growth of typical Melissococcus plutonius strains.
abstract: Melissococcus plutonius is a fastidious honeybee pathogen, and the addition of KH(2)PO(4) to culture medium is required for its growth. Using genome sequences and a newly developed vector, we showed that mutations in genes encoding Na(+)/H(+) antiporter and cation-transporting ATPase are involved in the potassium requirement for growth.
authors.affiliation: Bacterial and Parasitic Disease Research Division, National Institute of Animal Health, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, Japan. p1013dt@affrc.go.jp
authors.forename: Daisuke
authors.initials: D
authors.lastname: Takamatsu
authors.forename: Rie
authors.initials: R
authors.lastname: Arai
authors.forename: Tohru
authors.initials: T
authors.lastname: Miyoshi-Akiyama
authors.forename: Kayo
authors.initials: K
authors.lastname: Okumura
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Okura
authors.forename: Teruo
authors.initials: T
authors.lastname: Kirikae
authors.forename: Atsuko
authors.initials: A
authors.lastname: Kojima
authors.forename: Makoto
authors.initials: M
authors.lastname: Osaki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001516:Bees / Q000382:microbiology*; D000252:Calcium-Transporting ATPases / Q000235:genetics*; D002874:Chromosome Mapping; D019295:Computational Biology; D056569:Enterococcaceae / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D005816:Genetic Complementation Test; D005822:Genetic Vectors / Q000235:genetics; D016680:Genome, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D011188:Potassium / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics*
chemical_list: D017923:Sodium-Hydrogen Exchangers; D000252:Calcium-Transporting ATPases; D011188:Potassium