title: Transcriptomic profiles of high and low antibody responders to smallpox vaccine.
abstract: Despite its eradication over 30 years ago, smallpox (as well as other orthopox viruses) remains a pathogen of interest both in terms of biodefense and for its use as a vector for vaccines and immunotherapies. Here we describe the application of mRNA-Seq transcriptome profiling to understanding immune responses in smallpox vaccine recipients. Contrary to other studies examining gene expression in virally infected cell lines, we utilized a mixed population of peripheral blood mononuclear cells in order to capture the essential intercellular interactions that occur in vivo, and would otherwise be lost, using single cell lines or isolated primary cell subsets. In this mixed cell population we were able to detect expression of all annotated vaccinia genes. On the host side, a number of genes encoding cytokines, chemokines, complement factors and intracellular signaling molecules were downregulated upon viral infection, whereas genes encoding histone proteins and the interferon response were upregulated. We also identified a small number of genes that exhibited significantly different expression profiles in subjects with robust humoral immunity compared with those with weaker humoral responses. Our results provide evidence that differential gene regulation patterns may be at work in individuals with robust humoral immunity compared with those with weaker humoral immune responses.
authors.affiliation: Mayo Vaccine Research Group, Mayo Clinic, Rochester, MN, USA.
authors.forename: R B
authors.initials: RB
authors.lastname: Kennedy
authors.forename: A L
authors.initials: AL
authors.lastname: Oberg
authors.forename: I G
authors.initials: IG
authors.lastname: Ovsyannikova
authors.forename: I H
authors.initials: IH
authors.lastname: Haralambieva
authors.forename: D
authors.initials: D
authors.lastname: Grill
authors.forename: G A
authors.initials: GA
authors.lastname: Poland
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D002478:Cells, Cultured; D015967:Gene Expression Regulation, Viral / Q000235:genetics / Q000276:immunology; D006367:HeLa Cells; D006801:Humans; D056724:Immunity, Humoral / Q000235:genetics / Q000276:immunology; D007114:Immunization; D012900:Smallpox Vaccine / Q000276:immunology*; D059467:Transcriptome / Q000276:immunology*; D014616:Vaccinia virus / Q000235:genetics / Q000276:immunology*; D014709:Vero Cells
chemical_list: D000914:Antibodies, Viral; D012900:Smallpox Vaccine