title: Altered hepatic retinyl ester concentration and acyl composition in response to alcohol consumption.
abstract: Retinoids (vitamin A and its metabolites) are essential micronutrients that regulate many cellular processes. Greater than 70% of the body's retinoid reserves are stored in the liver as retinyl ester (RE). Chronic alcohol consumption induces depletion of hepatic retinoid stores, and the extent of this has been correlated with advancing stages of alcoholic liver disease. The goal of this study was to analyze the mechanisms responsible for depletion of hepatic RE stores by alcohol consumption. A change in the fatty-acyl composition of RE in alcohol-fed mice was observed within two weeks after the start of alcohol consumption. Specifically, alcohol-feeding was associated with a significant decline in hepatic retinyl palmitate levels; however, total RE levels were maintained by a compensatory increase in levels of usually minor RE species, particularly retinyl oleate. Our data suggests that alcohol feeding initially stimulates a futile cycle of RE hydrolysis and synthesis, and that the change in RE acyl composition is associated with a change in the acyl composition of hepatic phosphatidylcholine. The alcohol-induced change in RE acyl composition was specific to the liver, and was not seen in lung or white adipose tissue. This shift in hepatic RE fatty acyl composition is a sensitive indicator of alcohol consumption and may be an early biomarker for events associated with the development of alcoholic liver disease.
authors.affiliation: Department of Medicine, Columbia University, New York, NY, 10032, USA.
authors.forename: Robin D
authors.initials: RD
authors.lastname: Clugston
authors.forename: Hongfeng
authors.initials: H
authors.lastname: Jiang
authors.forename: Man Xia
authors.initials: MX
authors.lastname: Lee
authors.forename: Paul D
authors.initials: PD
authors.lastname: Berk
authors.forename: Ira J
authors.initials: IJ
authors.lastname: Goldberg
authors.forename: Li-Shin
authors.initials: LS
authors.lastname: Huang
authors.forename: William S
authors.initials: WS
authors.lastname: Blaner