title: Antioxidant properties and inhibitory effects of Satureja khozestanica essential oil on LDL oxidation induced-CuSO(4) in vitro.
abstract: OBJECTIVE
  To assess various antioxidative activities of Satureja khozestanica essential oil (SKE) and its effect on oxidation of low density lipoprotein (LDL) induced by CuSO4 in vitro by monitoring the formation of conjugated dienes and malondialdehyde (MDA).
  
  
  METHODS
  The formation of conjugated dienes, lag time and MDA were measured. Inhibition of this Cu-induced oxidation was studied in the presence of several concentrations of SKE. Also total antioxidant activity and free radical scavenging of SKE were determinated.
  
  
  RESULTS
  It was demonstrated that SKE was able to inhibit LDL oxidation and decrease the resistance of LDL against oxidation. The inhibitory effects of SKE on LDL oxidation were dose-dependent at concentrations ranging from 50 to 200 µg/mL. Total antioxidant capacity of SKE was (3.20±0.40) nmol of ascorbic acid equivalents/g SKE. The SKE showed remarkable scavenging activity on 2, 2-diphenyl-picrylhydrazyl, IC50 (5.30±0.11) ng/mL.
  
  
  CONCLUSIONS
  This study shows that SKE is a source of potent antioxidants and prevents the oxidation of LDL in vitro and it may be suitable for use in food and pharmaceutical applications.
authors.affiliation: Department of Biochemistry, Lorestan University of Medical Sciences, Khoramabad, Iran.
authors.forename: Shahrokh
authors.initials: S
authors.lastname: Bagheri
authors.forename: Hassan
authors.initials: H
authors.lastname: Ahmadvand
authors.forename: Ali
authors.initials: A
authors.lastname: Khosrowbeygi
authors.forename: Farshid
authors.initials: F
authors.lastname: Ghazanfari
authors.forename: Narges
authors.initials: N
authors.lastname: Jafari
authors.forename: Habibolah
authors.initials: H
authors.lastname: Nazem
authors.forename: Reza Haji
authors.initials: RH
authors.lastname: Hosseini
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D019327:Copper Sulfate / Q000737:chemistry; D006801:Humans; D008077:Lipoproteins, LDL / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D008315:Malondialdehyde / Q000737:chemistry; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology*; D010084:Oxidation-Reduction / Q000187:drug effects; D031362:Satureja / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D008077:Lipoproteins, LDL; D009822:Oils, Volatile; D008315:Malondialdehyde; D019327:Copper Sulfate
keywords: Antioxidant properties; Essential oil; Low density lipoprotein; Oxidation; Satureja khozestanica