title: Effects of buprenorphine and meloxicam analgesia on induced cerebral ischemia in C57BL/6 male mice.
abstract: Laboratory mice constitute an extensively used model to study the pathologic and functional outcomes of cerebral ischemic stroke. The middle cerebral artery occlusion (MCAO) model requires surgical intervention, which potentially can result in postsurgical pain and stress. In the present study, we investigated whether buprenorphine and meloxicam, at clinically relevant doses provided pain relief without altering infarct volume in male C57BL/6 mice. Common known side-effects of buprenorphine, including decreased food consumption, were noted after surgery in buprenorphine-treated mice, but these effects were brief and seen only during the treatment period. Fecal corticosterone metabolites did not differ significantly between the groups. In the present study, buprenorphine treatment did not alter infarction volume when compared with that of mice that did not receive analgesia. In contrast, meloxicam treatment significantly reduced infarct volume and may be a confounder if used as an analgesic during MCAO surgery. Furthermore, investigation of behavioral profiles by using an automated behavioral scoring system showed that rearing and sniffing behaviors decreased as infarct volume increased. This suggests that studies of exploratory behavior may aid in developing new markers of short-term stroke-related behavioral deficiencies in laboratory mice.
authors.affiliation: Department of Experimental Medicine, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark. kiroja@sund.ku.dk
authors.forename: Kirsten R
authors.initials: KR
authors.lastname: Jacobsen
authors.forename: Natasha
authors.initials: N
authors.lastname: Fauerby
authors.forename: Zindy
authors.initials: Z
authors.lastname: Raida
authors.forename: Otto
authors.initials: O
authors.lastname: Kalliokoski
authors.forename: Jann
authors.initials: J
authors.lastname: Hau
authors.forename: Flemming F
authors.initials: FF
authors.lastname: Johansen
authors.forename: Klas Sp
authors.initials: KS
authors.lastname: Abelson
mesh_terms: D000700:Analgesics / Q000627:therapeutic use*; D000818:Animals; D002545:Brain Ischemia / Q000188:drug therapy / Q000473:pathology*; D002047:Buprenorphine / Q000627:therapeutic use*; D003345:Corticosterone / Q000378:metabolism; D020244:Infarction, Middle Cerebral Artery / Q000188:drug therapy / Q000473:pathology; D008297:Male; D000077239:Meloxicam; D051379:Mice; D008810:Mice, Inbred C57BL; D010146:Pain / Q000188:drug therapy / Q000662:veterinary*; D059408:Pain Management / Q000662:veterinary; D012107:Research Design; D013843:Thiazines / Q000627:therapeutic use*; D013844:Thiazoles / Q000627:therapeutic use*
chemical_list: D000700:Analgesics; D013843:Thiazines; D013844:Thiazoles; D002047:Buprenorphine; D000077239:Meloxicam; D003345:Corticosterone