title: A polymerase chain reaction-based approach for evaluation of telomere-associated sequences and interstitial telomeric sequences.
abstract: Telomere minisatellites could be present in both terminal and internal chromosomal regions. We monitored the progress of BAL-31 nuclease digestion on Arabidopsis thaliana genomic DNA prepared by standard isolation techniques to verify its cleavage at terminal and internal genomic regions. A subtelomeric position of candidate sequences was validated using conventional polymerase chain reaction (PCR), combining the C-strand-specific telomeric primer with a subtelomeric reverse primer, and confirmed by quantitative PCR (qPCR) using sequence-specific primer pairs on DNA samples after BAL-31 digestion. qPCR amplification showed a gradual decrease in subtelomeric sequence signals, in contrast to interstitial telomeric sequences from pericentromere and control sequences.
authors.affiliation: Institute of Biophysics, Academy of Sciences of the Czech Republic, 612 65 Brno, Czech Republic. evin@ibp.cz
authors.forename: Eva
authors.initials: E
authors.lastname: Sýkorová
authors.forename: Miloslava
authors.initials: M
authors.lastname: Fojtová
authors.forename: Vratislav
authors.initials: V
authors.lastname: Peška
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D001483:Base Sequence; D018744:DNA, Plant / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D016615:Telomere / Q000235:genetics*
chemical_list: D018744:DNA, Plant