title: Molecular mechanisms of HMW glutenin subunits from 1S(l) genome of Aegilops longissima positively affecting wheat breadmaking quality.
abstract: A wheat cultivar "Chinese Spring" chromosome substitution line CS-1S(l)(1B), in which the 1B chromosome was substituted by 1S(l) from Aegilops longissima, was developed and found to possess superior dough and breadmaking quality. The molecular mechanism of its super quality conformation is studied in the aspects of high molecular glutenin genes, protein accumulation patterns, glutenin polymeric proteins, protein bodies, starch granules, and protein disulfide isomerase (PDI) and PDI-like protein expressions. Results showed that the introduced HMW-GS 1S(l)×2.3* and 1S(l)y16* in the substitution line possesses long repetitive domain, making both be larger than any known x- and y-type subunits from B genome. The introduced subunit genes were also found to have a higher level of mRNA expressions during grain development, resulting in more HMW-GS accumulation in the mature grains. A higher abundance of PDI and PDI-like proteins was observed which possess a known function of assisting disulfide bond formation. Larger HMW-GS deposited in protein bodies were also found in the substitution line. The CS substitution line is expected to be highly valuable in wheat quality improvement since the novel HMW-GS are located on chromosome 1S(l), making it possible to combine with the known superior D×5+Dy10 subunits encoded by Glu-D1 for developing high quality bread wheat.
authors.affiliation: Key Laboratory of Genetics and Biotechnology, College of Life Science, Capital Normal University, Beijing, China.
authors.forename: Shunli
authors.initials: S
authors.lastname: Wang
authors.forename: Zitong
authors.initials: Z
authors.lastname: Yu
authors.forename: Min
authors.initials: M
authors.lastname: Cao
authors.forename: Xixi
authors.initials: X
authors.lastname: Shen
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Li
authors.forename: Wujun
authors.initials: W
authors.lastname: Ma
authors.forename: H
authors.initials: H
authors.lastname: Weißgerber
authors.forename: Friedrich
authors.initials: F
authors.lastname: Zeller
authors.forename: Sai
authors.initials: S
authors.lastname: Hsam
authors.forename: Yueming
authors.initials: Y
authors.lastname: Yan
mesh_terms: D001939:Bread / Q000592:standards*; D002523:Edible Grain / Q000235:genetics / Q000254:growth & development; D056625:Endosperm / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D019143:Evolution, Molecular; D005433:Flour / Q000592:standards; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D018745:Genome, Plant; D005983:Glutens / Q000737:chemistry* / Q000235:genetics*; D054643:INDEL Mutation; D008970:Molecular Weight; D010802:Phylogeny; D010940:Plant Proteins / Q000378:metabolism; D030821:Plants, Genetically Modified; D006109:Poaceae / Q000737:chemistry* / Q000145:classification / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D021122:Protein Subunits; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014158:Transcription, Genetic; D014908:Triticum / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development
chemical_list: D010940:Plant Proteins; D021122:Protein Subunits; D005983:Glutens; C037302:glutenin