title: Differential in gel electrophoresis (DIGE) comparative proteomic analysis of macrophages cell cultures in response to perthamide C treatment.
abstract: Secondary metabolites contained in marine organisms disclose diverse pharmacological activities, due to their intrinsic ability to recognize bio-macromolecules, which alter their expression and modulate their function. Thus, the identification of the cellular pathways affected by marine natural products is crucial to provide important functional information concerning their mechanism of action at the molecular level. Perthamide C, a marine sponge metabolite isolated from the polar extracts of Theonella swinhoei and endowed with a broad and interesting anti-inflammatory profile, was found in a previous study to specifically interact with heat shock protein-90 and glucose regulated protein-94, also disclosing the ability to reduce cisplatin-mediated apoptosis. In this paper, we evaluated the effect of this compound on the whole proteome of murine macrophages cells by two-dimensional DIGE proteomics. Thirty-three spots were found to be altered in expression by at least 1.6-fold and 29 proteins were identified by LC ESI-Q/TOF-MS. These proteins are involved in different processes, such as metabolism, structural stability, protein folding assistance and gene expression. Among them, perthamide C modulates the expression of several chaperones implicated in the folding of proteins correlated to apoptosis, such as Hsp90 and T-complexes, and in this context our data shed more light on the cellular effects and pathways altered by this marine cyclo-peptide.
authors.affiliation: Department of Pharmacy, University of Salerno, Via Ponte don Melillo, 84084 Fisciano (SA), Italy. a.vilasi@ibp.cnr.it
authors.forename: Annalisa
authors.initials: A
authors.lastname: Vilasi
authors.forename: Maria Chiara
authors.initials: MC
authors.lastname: Monti
authors.forename: Alessandra
authors.initials: A
authors.lastname: Tosco
authors.forename: Simona
authors.initials: S
authors.lastname: De Marino
authors.forename: Luigi
authors.initials: L
authors.lastname: Margarucci
authors.forename: Raffaele
authors.initials: R
authors.lastname: Riccio
authors.forename: Agostino
authors.initials: A
authors.lastname: Casapullo
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002853:Chromatography, Liquid; D002945:Cisplatin / Q000494:pharmacology; D015180:Electrophoresis, Gel, Two-Dimensional; D005786:Gene Expression Regulation / Q000187:drug effects; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D013058:Mass Spectrometry; D051379:Mice; D010456:Peptides, Cyclic / Q000302:isolation & purification / Q000494:pharmacology*; D017510:Protein Folding / Q000187:drug effects; D020543:Proteome / Q000187:drug effects; D040901:Proteomics; D047232:Theonella / Q000737:chemistry*
chemical_list: D018841:HSP90 Heat-Shock Proteins; D010456:Peptides, Cyclic; D020543:Proteome; C574057:perthamide C; D002945:Cisplatin