title: Towards an integrated device that utilizes adherent cells in a micro-free-flow electrophoresis chip to achieve separation and biosensing.
abstract: We immobilized adherent human embryonic kidney (HEK) cells--which are able to trace adenosine triphosphate (ATP)--inside a microfluidic free-flow electrophoresis (μFFE) chip in order to develop an integrated device combining separation and biosensing capabilities. HEK 293 cells loaded with fluorescent calcium indicators were used as a model system to enable the spatially and temporally resolved detection of ATP. The local position of a 20 μM ATP stream was successfully visualized by these cells during free-flow electrophoresis, demonstrating the on-line detection capability of this technique towards native, unlabeled compounds.
authors.affiliation: Institut für Analytische Chemie, Universität Leipzig, Johannisallee 29, 04103 Leipzig, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Jezierski
authors.forename: Anke S
authors.initials: AS
authors.lastname: Klein
authors.forename: Christian
authors.initials: C
authors.lastname: Benz
authors.forename: Michael
authors.initials: M
authors.lastname: Schaefer
authors.forename: Stefan
authors.initials: S
authors.lastname: Nagl
authors.forename: Detlev
authors.initials: D
authors.lastname: Belder
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation; D002448:Cell Adhesion; D004586:Electrophoresis / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D005456:Fluorescent Dyes; D057809:HEK293 Cells; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods
chemical_list: D005456:Fluorescent Dyes; D000255:Adenosine Triphosphate