title: Selection of the best chemical pretreatment for lignocellulosic substrate Prosopis juliflora.
abstract: Pretreatment is a pre-requisite step in bioethanol production from lignocellulosic biomass required to remove lignin and increase the porosity of the substrate for saccharification. In the present study, chemical pretreatment of Prosopis juliflora was performed using alkali (NaOH, KOH, and NH3), reducing agents (Na2S2O4, Na2SO3) and NaClO2 in different concentration ranges at room temperature (30±2 °C) to remove maximum lignin with minimum sugar loss. Further, biphasic acid hydrolysis of the various pretreated substrates was performed at mild temperatures. Considering the amount of holocellulose hydrolyzed and inhibitors released during hydrolysis, best chemical pretreatment was selected. Among all the chemicals investigated, pretreatment with sodium dithionite at concentration of 2% (w/v) removed maximum lignin (80.46±1.35%) with a minimum sugar loss (2.56±0.021%). Subsequent biphasic acid hydrolysis of the sodium dithionite pretreated substrate hydrolyzed 40.09±1.22% of holocellulose and released minimum amount of phenolics (1.04±0.022 g/L) and furans (0.41±0.012 g/L) in the hydrolysate.
authors.affiliation: Department of Microbiology, Osmania University, Hyderabad 500 007, AP, India.
authors.forename: Shaik
authors.initials: S
authors.lastname: Naseeruddin
authors.forename: K
authors.initials: K
authors.lastname: Srilekha Yadav
authors.forename: L
authors.initials: L
authors.lastname: Sateesh
authors.forename: Ananth
authors.initials: A
authors.lastname: Manikyam
authors.forename: Suseelendra
authors.initials: S
authors.lastname: Desai
authors.forename: L
authors.initials: L
authors.lastname: Venkateswar Rao
mesh_terms: D000143:Acids / Q000494:pharmacology*; D000468:Alkalies / Q000494:pharmacology*; D000641:Ammonia / Q000494:pharmacology; D004227:Dithionite / Q000494:pharmacology; D006868:Hydrolysis / Q000187:drug effects; D006878:Hydroxides / Q000494:pharmacology; D008031:Lignin / Q000302:isolation & purification / Q000378:metabolism*; D017680:Potassium Compounds / Q000494:pharmacology; D029862:Prosopis / Q000737:chemistry* / Q000187:drug effects*; D019163:Reducing Agents / Q000494:pharmacology*; D012965:Sodium Chloride / Q000494:pharmacology; D012972:Sodium Hydroxide / Q000494:pharmacology; D013447:Sulfites / Q000494:pharmacology; D013464:Sulfuric Acids / Q000494:pharmacology
chemical_list: D000143:Acids; D000468:Alkalies; D006878:Hydroxides; D017680:Potassium Compounds; D019163:Reducing Agents; D013447:Sulfites; D013464:Sulfuric Acids; C036909:lignocellulose; D004227:Dithionite; D012965:Sodium Chloride; D012972:Sodium Hydroxide; D000641:Ammonia; D008031:Lignin; C025026:sodium sulfite; C029943:potassium hydroxide