title: In vitro labeling of neural stem cells with poly-L-lysine coated super paramagnetic nanoparticles for green fluorescent protein transfection.
abstract: The magnetic nanoparticle-based transfection method is a relatively new technique for delivery of functional genes to target tissues. We aimed to evaluate the transfection efficiency of rat neural stem cell (NSC) using poly-L-lysine hydrobromide (PLL)-coated super paramagnetic iron oxide nanoparticles (SPION).
  
  
  
  The SPION was prepared and coated with PLL as transfection agent and the transfection efficiency was evaluated in rat NSC using enhanced green fluorescent protein-N1 plasmid containing GFP as a reporter gene. NSC was incubated for 24 h in cell culture media containing 25 µg/ml SPION and in different concentrations of PLL (0.25, 0.50, 0.75, 1 and 2 µg/ml). Cell viability was determined before and after transfection for every concentration using Trypan blue assay. Characterization of prepared uncoated (SPION) and coated (SPION-PLL) complexes were evaluated by a transmission electron microscope and the zeta potential.
  
  
  
  PLL at 0.75 μg/ml showed optimal results with 25 μg/ml SPION concentration compared with other PLL concentrations (0.25, 0.50, 1 and 2 μg/ml). The 18% efficiency of the transfected cells showed green fluorescence.
  
  
  
  Transfection with SPION is an efficient, non-viral gene transfere method.
authors.affiliation: Dept. of Nanobiotechnology, Faculty of Biological sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Salim
authors.initials: S
authors.lastname: Albukhaty
authors.affiliation: Dept. of Nanobiotechnology, Faculty of Biological sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Naderi-Manesh
authors.affiliation: 2Shefa Neurosciences Research Center, Khatam Al-Anbia Hospital, Tehran, Iran
authors.forename: Taki
authors.initials: T
authors.lastname: Tiraihi
mesh_terms: D000818:Animals; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003911:Dextrans; D049452:Green Fluorescent Proteins / Q000378:metabolism*; D058185:Magnetite Nanoparticles; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D058953:Neural Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D011107:Polylysine / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling; D014162:Transfection
chemical_list: D003911:Dextrans; D058185:Magnetite Nanoparticles; D049452:Green Fluorescent Proteins; D011107:Polylysine; C065507:ferumoxides
keywords: Neural Stem cells; Nanoparticles; Transfection