title: The oestrogenic and anti-platelet activities of dihydrobenzofuroisocoumarins and homoisoflavonoids from Liriope platyphylla roots.
abstract: The ethanolic extract of Liriope platyphylla (Liliaceae) roots showed potential oestrogenic and anti-platelet activities. Twenty-six compounds were isolated and classified as 10 skeletons, including two unusual new dihydrobenzofuroisocoumarins, (+)-platyphyllarin A (1) and B (2), one new butanoate, ethyltributanoate (3), and two new homoisoflavanones, (-)-liriopein A (4) and B (5), along with 21 known compounds, including six homoisoflavonoids, one chalcone, six amides, one lignan, one fatty acid derivative, one alkaloid, three benzenoids, and two steroids. The biosynthetic pathway of compounds 1 and 2 was proposed in the current investigation. The oestrogenic activity of the isolates was evaluated utilising the pER8:GUS reporter assay system in transgenic Arabidopsis plant as well as the SEAP reporter assay system in MCF-7 breast cancer cell-line; the anti-platelet activity was evaluated using the anti-platelet aggregation assay. Several components exhibited significant oestrogenic and anti-platelet activities; demonstrating for the first time the potential use of L. platyphylla as a nutritional supplement for cardiovascular and endocrine diseases.
authors.affiliation: Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Yu-Chi
authors.initials: YC
authors.lastname: Tsai
authors.forename: Shang-Yu
authors.initials: SY
authors.lastname: Chiang
authors.forename: Mohamed
authors.initials: M
authors.lastname: El-Shazly
authors.forename: Chin-Chung
authors.initials: CC
authors.lastname: Wu
authors.forename: Ludger
authors.initials: L
authors.lastname: Beerhues
authors.forename: Wan-Chun
authors.initials: WC
authors.lastname: Lai
authors.forename: Shou-Fang
authors.initials: SF
authors.lastname: Wu
authors.forename: Ming-Hong
authors.initials: MH
authors.lastname: Yen
authors.forename: Yang-Chang
authors.initials: YC
authors.lastname: Wu
authors.forename: Fang-Rong
authors.initials: FR
authors.lastname: Chang
mesh_terms: D001792:Blood Platelets / Q000187:drug effects* / Q000502:physiology; D045744:Cell Line, Tumor; D004967:Estrogens / Q000737:chemistry; D006801:Humans; D007529:Isoflavones / Q000737:chemistry / Q000494:pharmacology*; D019668:Liliaceae / Q000737:chemistry*; D031424:Liriope Plant / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D015539:Platelet Activation / Q000187:drug effects
chemical_list: D004967:Estrogens; D007529:Isoflavones; D010936:Plant Extracts