title: Prevalence of Porphyromonas gingivalis four rag locus genotypes in patients of orthodontic gingivitis and periodontitis.
abstract: Porphyromonas gingivalis is considered as a major etiological agent in periodontal diseases and implied to result in gingival inflammation under orthodontic appliance. rag locus is a pathogenicity island found in Porphyromonas gingivalis. Four rag locus variants are different in pathogenicity of Porphyromonas gingivalis. Moreover, there are different racial and geographic differences in distribution of rag locus genotypes. In this study, we assessed the prevalence of Porphyromonas gingivalis and rag locus genotypes in 102 gingival crevicular fluid samples from 57 cases of gingivitis patients with orthodontic appliances, 25 cases of periodontitis patients and 20 cases of periodontally healthy people through a 16S rRNA-based PCR and a multiplex PCR. The correlations between Porphyromona.gingivalis/rag locus and clinical indices were analyzed. The prevalence of Porphyromonas gingivalis and rag locus genes in periodontitis group was the highest among three groups and higher in orthodontic gingivitis than healthy people (p<0.01). An obviously positive correlation was observed between the prevalence of Porphyromonas gingivalis/rag locus and gingival index. rag-3 and rag-4 were the predominant genotypes in the patients of orthodontic gingivitis and mild-to-moderate periodontitis in Shandong. Porphyromonas.gingivalis carrying rag-1 has the strong virulence and could be associated with severe periodontitis.
authors.affiliation: Pediatric Research Institute, Qilu Children's Hospital of Shandong University, Ji'nan, Shandong, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Yujie
authors.initials: Y
authors.lastname: Zhang
authors.forename: Lili
authors.initials: L
authors.lastname: Wang
authors.forename: Yang
authors.initials: Y
authors.lastname: Guo
authors.forename: Shuiqing
authors.initials: S
authors.lastname: Xiao
mesh_terms: D000293:Adolescent; D000328:Adult; D016866:Bacteroidaceae Infections / Q000453:epidemiology / Q000382:microbiology*; D002648:Child; D005260:Female; D005798:Genes, Bacterial; D056426:Genetic Loci; D005838:Genotype; D005891:Gingivitis / Q000453:epidemiology / Q000382:microbiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000453:epidemiology / Q000382:microbiology*; D016966:Porphyromonas gingivalis / Q000235:genetics*; D015995:Prevalence; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D055815:Young Adult
chemical_list: D012336:RNA, Ribosomal, 16S