title: Coal-burning endemic fluorosis is associated with reduced activity in antioxidative enzymes and Cu/Zn-SOD gene expression.
abstract: To study the effect of fluorine on the oxidative stress in coal-burning fluorosis, we investigated the environmental characteristics of coal-burning endemic fluorosis combined with fluorine content surveillance in air, water, food, briquette, and clay binder samples from Bijie region, Guizhou Province, southwest of China. The activities of antioxidant enzymes including copper/zinc superoxide dismutase (Cu/Zn-SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and level of lipid peroxidation such as malondialdehyde (MDA) were measured in serum samples obtained from subjects residing in the Bijie region. Expression of the Cu/Zn-SOD gene was assessed by quantitative reverse transcriptase PCR (qRT-PCR). Our results showed that people suffering from endemic fluorosis (the high and low exposure groups) had much higher MDA level. Their antioxidant enzyme activities and Cu/Zn-SOD gene expression levels were lower when compared to healthy people (the control group). Fluorosis can decrease the activities of antioxidant enzymes, which was associated with exposure level of fluorine. Down-regulation of Cu/Zn-SOD expression may play an important role in the aggravation of oxidative stress in endemic fluorosis.
authors.affiliation: Department of Prevention Medicine, School of Public Health, Zunyi Medical College, Zunyi, 563003, People's Republic of China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.forename: Kang-ping
authors.initials: KP
authors.lastname: Cui
authors.forename: Yuan-yuan
authors.initials: YY
authors.lastname: Xu
authors.forename: Yan-ling
authors.initials: YL
authors.lastname: Gao
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Da-sheng
authors.initials: DS
authors.lastname: Li
authors.forename: Xiao-lei
authors.initials: XL
authors.lastname: Li
authors.forename: Hou-jin
authors.initials: HJ
authors.lastname: Huang
mesh_terms: D000293:Adolescent; D000328:Adult; D000393:Air Pollutants / Q000032:analysis; D016022:Case-Control Studies; D002374:Catalase / Q000097:blood; D002681:China / Q000453:epidemiology; D003031:Coal; D003032:Coal Mining; D060766:Drinking Water / Q000032:analysis / Q000737:chemistry; D004781:Environmental Exposure / Q000009:adverse effects / Q000032:analysis*; D004798:Enzymes / Q000097:blood*; D005260:Female; D005459:Fluorides / Q000032:analysis; D005461:Fluorine / Q000009:adverse effects / Q000652:urine; D009050:Fluorosis, Dental / Q000201:enzymology* / Q000453:epidemiology / Q000235:genetics*; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000235:genetics*; D055815:Young Adult
chemical_list: D000393:Air Pollutants; D003031:Coal; D060766:Drinking Water; D004798:Enzymes; D005461:Fluorine; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005459:Fluorides