title: Differential expression and regulation of Cryab in mouse uterus during preimplantation period.
abstract: The aim of this study was to examine the expression and regulation of the crystallin, alpha B (Cryab) gene in mouse uterus during the peri-implantation period by in situ hybridization and real-time PCR. There was no detectable Cryab mRNA signal on days 1-4 of pregnancy. On day 5 of pregnancy when embryo implanted, a high level of Cryab mRNA signal was found in the subluminal stroma surrounding the implanting blastocyst. On days 6-8, Cryab mRNA was strongly expressed in the primary decidua. By real-time PCR, a high level of Cryab expression was detected on days 7 and 8 of pregnancy, although Cryab expression was seen from days 1 to 8. Under in vivo and in vitro artificial decidualization, Cryab expression was significantly elevated. Compared with the progesterone-primed delayed implantation uterus, a high level of Cryab mRNA expression was observed in estrogen-activated implantation uterus. In the uterine stromal cells, cAMP, estrogen, and progesterone could induce the expression of Cryab gene. In the ovariectomized mouse uterus, estrogen could also induce the expression of Cryab while progesterone inhibited its expression. Our data suggest that Cryab may play an important role during mouse embryo implantation and decidualization and that estrogen and progesterone can regulate the expression of Cryab gene.
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun 130062, People's Republic of China.
authors.forename: Xue-Chao
authors.initials: XC
authors.lastname: Tian
authors.forename: Qu-Yuan
authors.initials: QY
authors.lastname: Wang
authors.forename: Dang-Dang
authors.initials: DD
authors.lastname: Li
authors.forename: Shou-Tang
authors.initials: ST
authors.lastname: Wang
authors.forename: Zhan-Qing
authors.initials: ZQ
authors.lastname: Yang
authors.forename: Bin
authors.initials: B
authors.lastname: Guo
authors.forename: Zhan-Peng
authors.initials: ZP
authors.lastname: Yue
mesh_terms: D000818:Animals; D003656:Decidua / Q000187:drug effects / Q000378:metabolism*; D010064:Embryo Implantation / Q000187:drug effects; D010065:Embryo Implantation, Delayed / Q000187:drug effects; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism*; D015914:Estrogen Replacement Therapy; D004967:Estrogens / Q000494:pharmacology; D005260:Female; D046910:Fertile Period / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D017403:In Situ Hybridization; D051379:Mice; D010052:Ovariectomy / Q000009:adverse effects; D010929:Placentation / Q000187:drug effects; D011247:Pregnancy; D011374:Progesterone / Q000494:pharmacology; D011372:Progestins / Q000494:pharmacology; D011555:Pseudopregnancy / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D017154:Stromal Cells / Q000187:drug effects / Q000378:metabolism; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*; D038203:alpha-Crystallin B Chain / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism
chemical_list: C525420:Cryab protein, mouse; D004967:Estrogens; D011372:Progestins; D012333:RNA, Messenger; D038203:alpha-Crystallin B Chain; D011374:Progesterone