title: Simultaneous quantification of paeoniflorin, nobiletin, tangeretin, liquiritigenin, isoliquiritigenin, liquiritin and formononetin from Si-Ni-San extract in rat plasma and tissues by liquid chromatography-tandem mass spectrometry.
abstract: In this study, a sensitive and reliable liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the determination of seven bioactive components including paeoniflorin, nobiletin, tangeretin, liquiritigenin, isoliquiritigenin, liquiritin and formononetin in rat plasma and tissues after oral administration of Si-Ni-San extract using astragaloside IV as internal standard (IS). The plasma and tissue samples were extracted by solid-phase extraction. Chromatographic separation was accomplished on a C18 column with a multiple-step gradient elution. The quantification was obtained by scanning with multiple reaction monitoring via an electrospray ionization source that was operated by switching between the positive and negative modes in two MS/MS scan segments. Full validation of the assay was implemented. In conclusion, this method demonstrated good linearity and specificity. The lower limits of quantification for the analytes were <7.5 ng/mL. Intra- and inter-day precisions (RSD) were <12.5% and accuracy (RE) ranged from -10.2 to 7.3%. The average recoveries of the analytes from rat plasma and tissues were >65.2% and 58.6%, respectively. The validated method was further applied to the determination of actual rat plasma and tissues after oral administration of Si-Ni-San extract. The results provided a meaningful basis for the clinical application of this prescription.
authors.affiliation: Laboratory of Pharmaceutics, Jiangsu Key laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, 210023, People's Republic of China.
authors.forename: Tianxue
authors.initials: T
authors.lastname: Li
authors.forename: Zhixiang
authors.initials: Z
authors.lastname: Yan
authors.forename: Chen
authors.initials: C
authors.lastname: Zhou
authors.forename: Jian
authors.initials: J
authors.lastname: Sun
authors.forename: Chuan
authors.initials: C
authors.lastname: Jiang
authors.forename: Xinghao
authors.initials: X
authors.lastname: Yang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001923:Brain Chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000032:analysis* / Q000493:pharmacokinetics*; D047309:Flavones / Q000032:analysis* / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D016018:Least-Squares Analysis; D008099:Liver / Q000737:chemistry; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013154:Spleen / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014018:Tissue Distribution
chemical_list: D004365:Drugs, Chinese Herbal; D047309:Flavones; C510945:shigyaku-san
keywords: LC-ESI-MS/MS; Si-Ni-San; multiple reaction monitoring; pharmacokinetics; quantification