title: Migration patterns and cell functions of adipose-derived stromal cells on self-assembled monolayers with different functional groups.
abstract: Microenvironments provide cues to stem cells and induce signals to direct their fate. With a view toward further understanding the correlation between surface chemistry and cell functions of stem cells, adipose-derived stromal cells (ADSCs) and self-assembled monolayers terminated with four different functional groups (-CH3, -NH2, -COOH and -OH) were used to assess cell adhesion, migration and differentiation potential in short-term incubation. Quantitative time-lapse microscopic analysis revealed that migration speed and patterns were strongly regulated by surface chemistry. ADSCs showed a mesenchymal migration pattern on the -COOH-modified surface. In contrast, cells on the -CH3-modified surface displayed an amoeboid-like migration pattern. Cell-migration speeds on the chemically-modified surfaces followed the sequence (by tail-groups): -CH3>-COOH>-OH>-NH2. After 1 day of incubation, ADSCs showed a round compact shape and adipogenic differentiation potential on the -CH3-modified surface. The round compact shape and extremely different migration pattern of ADSCs on -CH3 surfaces were attributed to the lower amount of exposed cell-binding domains of adsorbed proteins. ADSCs exhibited spindle-like shape and higher Collagen II expression on the -COOH-modified surface and well-spread morphology and higher Runx2 expression were observed on the -NH2- and -OH-modified surfaces. Surface chemistry presented a strong influence on cell functions of ADSCs, including cell adhesion, migration and mRNA expression in short-term incubation.
authors.affiliation: Department of Materials Science and Engineering , National Cheng Kung University, 1 University Road, Tainan, Taiwan.
authors.forename: Hsiao-Feng
authors.initials: HF
authors.lastname: Chieh
authors.forename: Fong-Chin
authors.initials: FC
authors.lastname: Su
authors.forename: Sheng-Che
authors.initials: SC
authors.lastname: Lin
authors.forename: Meng-Ru
authors.initials: MR
authors.lastname: Shen
authors.forename: Jiunn-Der
authors.initials: JD
authors.lastname: Liao
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D002454:Cell Differentiation; D002465:Cell Movement; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000648:ultrastructure; D005109:Extracellular Matrix / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017154:Stromal Cells / Q000166:cytology* / Q000378:metabolism; D013499:Surface Properties; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials