title: Silencing heme oxygenase-1 gene expression in retinal pigment epithelial cells inhibits proliferation, migration and tube formation of cocultured endothelial cells.
abstract: Heme oxygenase-1 (HO-1) plays an important role in the vasculature and in the angiogenesis of tumors, wounds and other environments. Retinal pigment epithelial (RPE) cells and choroidal endothelial cells (CECs) are the main cells involved in choroidal neovascularization (CNV), a process in which hypoxia plays an important role. Our aim was to evaluate the role of human RPE-cell HO-1 in the angiogenic activities of cocultured endothelial cells under hypoxia. Small interfering RNA (siRNA) for HO-1 was transfected into human RPE cell line ARPE-19, and zinc protoporphyrin (ZnPP) was used to inhibit HO-1 activity. Knockdown of HO-1 expression and inhibition of HO-1 activity resulted in potent reduction of the expression of vascular endothelial growth factor (VEGF) under hypoxia. Furthermore, knockdown of HO-1 suppressed the proliferation, migration and tube formation of cocultured endothelial cells. These findings indicated that HO-1 might have an angiogenic effect in CNV through modulation of VEGF expression and might be a potential target for treating CNV.
authors.affiliation: Ophthalmology Hospital, The First Affiliated Hospital of Harbin Medical University, 23 Youzheng Street, Harbin 150001, China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Zhang
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Lu
authors.forename: Makoto
authors.initials: M
authors.lastname: Matsukura
authors.forename: Jien
authors.initials: J
authors.lastname: Zhao
authors.forename: Makoto
authors.initials: M
authors.lastname: Shinohara
mesh_terms: D001483:Base Sequence; D015153:Blotting, Western; D002460:Cell Line; D002465:Cell Movement; D049109:Cell Proliferation; D018920:Coculture Techniques; D017931:DNA Primers; D020868:Gene Silencing; D051547:Heme Oxygenase-1 / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction; D034741:RNA, Small Interfering / Q000235:genetics; D055213:Retinal Pigment Epithelium / Q000166:cytology / Q000201:enzymology*
chemical_list: D017931:DNA Primers; D034741:RNA, Small Interfering; D051547:Heme Oxygenase-1