title: Silica nanorattle with enhanced protein loading: a potential vaccine adjuvant.
abstract: Nanoparticles are excellent carriers for drug and protein, and have the potential to be used in vaccine delivery system. Here, we prepared different structures silica nanoparticles such as silica nanorattles (SNs), mesoporous silica nanoparticles (MSNs) and solid silica nanoparticles (SSNs), and chosen ovalbumin (OVA) as model protein to study the potential application of silica nanoparticles in protein vaccine delivery system. The results showed that silica nanoparticles were efficient in protein loading and dependent on structure, size and incubation medium. According to the three structure particles, SNs were favorable to be used as protein carriers. Furthermore, we proved low cytotoxicity of silica nanorattle on RAW 264.7 cell line and biocompatibility in vivo. In addition, SNs was capable to up-regulate the humoral immunity reaction when mice were vaccinated with SNs-OVA formulation. Taken together, SNs was excellent carriers for protein vaccine and has the potential to be used as adjuvant.
authors.affiliation: Laboratory of Controllable Preparation and Application of Nanomaterials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China.
authors.forename: Tianlong
authors.initials: T
authors.lastname: Liu
authors.forename: Huiyu
authors.initials: H
authors.lastname: Liu
authors.forename: Changhui
authors.initials: C
authors.lastname: Fu
authors.forename: Linlin
authors.initials: L
authors.lastname: Li
authors.forename: Dong
authors.initials: D
authors.lastname: Chen
authors.forename: Yanqi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Fangqiong
authors.initials: F
authors.lastname: Tang
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D002460:Cell Line; D002470:Cell Survival; D004337:Drug Carriers / Q000737:chemistry*; D005260:Female; D007074:Immunoglobulin G / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010047:Ovalbumin / Q000008:administration & dosage* / Q000276:immunology; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D000276:Adjuvants, Immunologic; D004337:Drug Carriers; D007074:Immunoglobulin G; D012822:Silicon Dioxide; D010047:Ovalbumin