title: Efficient methods to generate reproducible mass spectra in matrix-assisted laser desorption ionization of peptides.
abstract: In our previous matrix-assisted laser desorption ionization (MALDI) studies of peptides, we found that their mass spectra were virtually determined by the effective temperature in the early matrix plume, Tearly, when samples were rather homogeneous. This empirical rule allowed acquisition of quantitatively reproducible spectra. A difficulty in utilizing this rule was the complicated spectral treatment needed to get Tearly. In this work, we found another empirical rule that the total number of particles hitting the detector, or TIC, was a good measure of the spectral temperature and, hence, selection of spectra with the same TIC resulted in reproducible spectra. We also succeeded in obtaining reproducible spectra throughout a measurement by controlling TIC near a preset value through feedback adjustment of laser pulse energy. Both TIC selection and TIC control substantially reduced the shot-to-shot spectral variation in a spot, spot-to-spot variation in a sample, and even sample-to-sample variation in MALDI using α-cyano-4-hydroxycinnamic acid or 2,5-dihydroxybenzoic acid as matrix. Based on the utilization of acquired data, TIC control was more efficient than TIC selection by an order of magnitude. Both techniques produced calibration curves with excellent linearity, suggesting their utility in quantification of peptides.
authors.affiliation: Department of Chemistry, Seoul National University, Seoul, 151-742, Korea.
authors.forename: Sung Hee
authors.initials: SH
authors.lastname: Ahn
authors.forename: Kyung Man
authors.initials: KM
authors.lastname: Park
authors.forename: Yong Jin
authors.initials: YJ
authors.lastname: Bae
authors.forename: Myung Soo
authors.initials: MS
authors.lastname: Kim
mesh_terms: D002138:Calibration; D003373:Coumaric Acids / Q000737:chemistry; D005841:Gentisates / Q000737:chemistry; D007477:Ions / Q000032:analysis / Q000737:chemistry; D010455:Peptides / Q000032:analysis / Q000737:chemistry*; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*; D013696:Temperature
chemical_list: D003373:Coumaric Acids; D005841:Gentisates; D007477:Ions; D010455:Peptides; C007175:alpha-cyano-4-hydroxycinnamate; C010925:2,5-dihydroxybenzoic acid