title: Chemical composition and nematicidal activity of essential oil of Agastache rugosa against Meloidogyne incognita.
abstract: The aim of this research was to determine the chemical composition and nematicidal activity of essential oil of Agastache rugosa flowering aerial parts against the root knot nematode, Meloidogyne incognita, and to isolate and identify any nematicidal constituents from the essential oil. The essential oil of A. rugosa aerial parts was obtained by hydrodistillation and analyzed by GC-FID and GC-MS. A total of 37 components of the essential oil were identified, with the principal compounds being methyleugenol (50.51%), estragole (8.55%), and eugenol (7.54%), followed by thymol (3.62%), pulegone (2.56%), limonene (2.49%) and caryophyllene (2.38%). Based on bioactivity-guided fractionation, the three active constituents were isolated from the essential oil and identified as methyleugenol, estragole and eugenol. The essential oil of A. rugosa exhibited strong nematicidal activity against M. incognita, with a LC50 value of 47.3 μg/mL. The components eugenol (LC50 = 66.6 μg/mL) and methyleugenol (LC50 = 89.4 μg/mL) exhibited stronger nematicidal activity against M. incognita (LC50 = 185.9 μg/mL). The results indicate that the essential oil of A. rugosa aerial parts and its constituent compounds have potential for development into natural nematicides for control of the root knot nematode.
authors.affiliation: Department of Entomology, China Agricultural University, Haidian District, Beijing 100193, China. hqliaau@163.com
authors.forename: He Qin
authors.initials: HQ
authors.lastname: Li
authors.forename: Qi Zhi
authors.initials: QZ
authors.lastname: Liu
authors.forename: Zhi Long
authors.initials: ZL
authors.lastname: Liu
authors.forename: Shu Shan
authors.initials: SS
authors.lastname: Du
authors.forename: Zhi Wei
authors.initials: ZW
authors.lastname: Deng
mesh_terms: D031327:Agastache / Q000737:chemistry*; D000081248:Allylbenzene Derivatives; D000818:Animals; D000840:Anisoles / Q000737:chemistry; D000969:Antinematodal Agents / Q000737:chemistry* / Q000494:pharmacology; D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes / Q000737:chemistry; D005054:Eugenol / Q000737:chemistry; D008401:Gas Chromatography-Mass Spectrometry; D007928:Lethal Dose 50; D000077222:Limonene; D039821:Monoterpenes / Q000737:chemistry; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology; D010938:Plant Oils / Q000737:chemistry* / Q000494:pharmacology; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes / Q000737:chemistry; D013729:Terpenes / Q000737:chemistry; D013943:Thymol / Q000737:chemistry; D014431:Tylenchoidea / Q000187:drug effects*
chemical_list: D000081248:Allylbenzene Derivatives; D000840:Anisoles; D000969:Antinematodal Agents; D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes; D039821:Monoterpenes; D009822:Oils, Volatile; D010938:Plant Oils; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes; D013729:Terpenes; D013943:Thymol; D005054:Eugenol; C039648:pulegone; D000077222:Limonene; C007633:estragole; C024714:caryophyllene