title: Human papillomavirus was not detected by PCR using multiple consensus primer sets in esophageal adenocarcinomas in Chinese patients.
abstract: The role of human papillomavirus (HPV) infection in the development of esophageal squamous cell carcinoma is well established; however, there are few reports on the role of HPV in esophageal adenocarcinoma. To evaluate the putative role of HPV infection in esophageal adenocarcinoma, 57 formalin-fixed, paraffin-embedded esophageal adenocarcinoma specimens were collected from four hospitals in Shanghai and Anyang, China, between 1999 and 2008. HPV DNA was analyzed using PCR with multiple sets of consensus primers for HPV, GP5+/6+, CPI/CPIIG, SPF10, pU-1M/pU2R, and pU31B/pU2R. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), the internal control, was amplified successfully in all 57 specimens. However, HPV amplification was not detected in any specimens with any of the consensus primer sets used. The present study indicates that HPV infection is not likely to be a major factor in the etiology of esophageal adenocarcinoma in the Chinese population.
authors.affiliation: Department of Nephrology, The Affiliated People's Hospital of Jiangsu University, Zhenjiang, Jiangsu Province, China.
authors.forename: Songtao
authors.initials: S
authors.lastname: Feng
authors.forename: Jianming
authors.initials: J
authors.lastname: Zheng
authors.forename: Xiang
authors.initials: X
authors.lastname: Du
authors.forename: Yunshan
authors.initials: Y
authors.lastname: Tan
authors.forename: Haijun
authors.initials: H
authors.lastname: Yang
authors.forename: Hongxia
authors.initials: H
authors.lastname: Zhang
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000230:Adenocarcinoma / Q000150:complications / Q000175:diagnosis* / Q000208:ethnology / Q000821:virology; D000293:Adolescent; D000328:Adult; D000368:Aged; D044466:Asian People; D002294:Carcinoma, Squamous Cell / Q000150:complications / Q000175:diagnosis* / Q000208:ethnology / Q000821:virology; D003127:Colposcopy; D017931:DNA Primers / Q000235:genetics; D004938:Esophageal Neoplasms / Q000150:complications / Q000175:diagnosis* / Q000208:ethnology / Q000821:virology; D005260:Female; D052162:Human papillomavirus 16 / Q000235:genetics* / Q000302:isolation & purification; D052161:Human papillomavirus 18 / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000150:complications / Q000175:diagnosis* / Q000208:ethnology / Q000821:virology; D016133:Polymerase Chain Reaction; D011446:Prospective Studies; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D012680:Sensitivity and Specificity; D012720:Severity of Illness Index; D002578:Uterine Cervical Dysplasia / Q000150:complications / Q000175:diagnosis* / Q000208:ethnology / Q000821:virology
chemical_list: D017931:DNA Primers; D012333:RNA, Messenger; D012367:RNA, Viral