title: Development of a novel vaccine against canine parvovirus infection with a clinical isolate of the type 2b strain.
abstract: OBJECTIVE
  In spite of an extensive vaccination program, parvoviral infections still pose a major threat to the health of dogs.
  
  
  METHODS
  We isolated a novel canine parvovirus (CPV) strain from a dog with enteritis. Nucleotide and amino acid sequence analysis of the isolate showed that it is a novel type 2b CPV with asparagine at the 426th position and valine at the 555th position in VP2. To develop a vaccine against CPV infection, we passaged the isolate 4 times in A72 cells.
  
  
  RESULTS
  The attenuated isolate conferred complete protection against lethal homologous CPV infection in dogs such that they did not develop any clinical symptoms, and their antibody titers against CPV were significantly high at 7-11 days post infection.
  
  
  CONCLUSIONS
  These results suggest that the virus isolate obtained after passaging can be developed as a novel vaccine against paroviral infection.
authors.affiliation: Department of Veterinary Infectious Diseases, College of Veterinary Medicine, Konkuk University, Seoul, Korea.
authors.forename: Seon Ah
authors.initials: SA
authors.lastname: Park
authors.forename: Seung-Yong
authors.initials: SY
authors.lastname: Park
authors.forename: Chang-Seon
authors.initials: CS
authors.lastname: Song
authors.forename: In-Soo
authors.initials: IS
authors.lastname: Choi
authors.forename: Hwi Yool
authors.initials: HY
authors.lastname: Kim
authors.forename: Joong-Bok
authors.initials: JB
authors.lastname: Lee
authors.forename: Nak-Hyung
authors.initials: NH
authors.lastname: Lee
keywords: Canine parvoviral infection; Clinical isolate; Dogs; Novel type 2b; Vaccines