title: Design and evaluation of theranostic perfluorocarbon particles for simultaneous antigen-loading and ¹⁹F-MRI tracking of dendritic cells.
abstract: Perfluorocarbon (PFC) particles are currently on the rise as cell labeling agents for ¹⁹F-MRI tracking of dendritic cell (DC)-based vaccines. In this work, we design theranostic PFC particles for single-step loading of DCs with both antigenic protein and with a liquid PFC for ¹⁹F-MRI detection of the antigen-loaded cells. Upon addition to DCs in vitro, the antigen-loaded PFC particles are efficiently internalized, resulting in intracellular presence of up to 40 pmol ¹⁹F atoms per cell. At the same time, the DCs become loaded with antigenic proteins, that can be efficiently processed, without important effects on cell viability or altering the DC's phenotype and the cell's capacity to respond to danger signals. In addition, antigen-loaded PFC particle containing DCs are capable of inducing extensive proliferation of antigen-specific CD8⁺ T cells in vitro. Importantly, the antigen-coated PFC particles allow in vitro ¹⁹F-MRI-based detection of the antigen-containing DCs with detection limits as low as 10³ cells μl⁻¹. The dual-modality characteristics of the designed particles could assure that only those DCs that have taken up the antigen, and hence are responsible for an immune response, are traceable via ¹⁹F-MRI. Taken together, these novel dual-modality particles represent an interesting strategy in the development of a traceable DC vaccine.
authors.affiliation: Lab for General Biochemistry and Physical Pharmacy, Faculty of Pharmacy, Ghent University, Harelbekestraat 72, 9000 Ghent, Belgium.
authors.forename: Heleen
authors.initials: H
authors.lastname: Dewitte
authors.forename: Bart
authors.initials: B
authors.lastname: Geers
authors.forename: Sayuan
authors.initials: S
authors.lastname: Liang
authors.forename: Uwe
authors.initials: U
authors.lastname: Himmelreich
authors.forename: Jo
authors.initials: J
authors.lastname: Demeester
authors.forename: Stefaan C
authors.initials: SC
authors.lastname: De Smedt
authors.forename: Ine
authors.initials: I
authors.lastname: Lentacker
mesh_terms: D000818:Animals; D000941:Antigens / Q000008:administration & dosage* / Q000032:analysis / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D002470:Cell Survival; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D005260:Female; D005466:Fluorocarbons / Q000737:chemistry*; D008213:Lymphocyte Activation; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D008863:Microspheres; D010047:Ovalbumin / Q000008:administration & dosage* / Q000032:analysis / Q000276:immunology
chemical_list: D000941:Antigens; D005466:Fluorocarbons; D010047:Ovalbumin