title: Generation of mouse models of lymphoid neoplasm using retroviral gene transduction of in vitro-induced germinal center B and T cells.
abstract: Evidence is accumulating that hematologic malignancies develop following acquisition of multiple genetic changes. Despite providing many insights into the way by which given genetic changes contribute to the development of disease, the generation of animal models is often laborious. We show a simplified method that allows the retroviral transduction of genes of interest into mouse B or T cells, thus leading to the rapid generation of models of lymphoid neoplasm in mice. Specifically, germinal center B cells induced in vitro from naive mouse B cells and infected with retroviruses for Myc and Bcl2 rapidly developed a neoplasm of immunoglobulin-expressing mature B cells in transplanted mice. Likewise, T cells induced in vitro from immature hematopoietic cells and infected with retroviruses for Myc, Bcl2, and Ccnd1 rapidly developed CD4(+)CD8(-) and CD4(+)CD8(+) T cell neoplasm in transplanted mice. These findings support the use of our simplified method as a versatile tool for lymphoma research.
authors.affiliation: Division of Molecular Medicine, Aichi Cancer Center Research Institute, Nagoya, Japan.
authors.forename: Kotaro
authors.initials: K
authors.lastname: Arita
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Maeda-Kasugai
authors.forename: Koichi
authors.initials: K
authors.lastname: Ohshima
authors.forename: Shinobu
authors.initials: S
authors.lastname: Tsuzuki
authors.forename: Miyuki
authors.initials: M
authors.lastname: Suguro-Katayama
authors.forename: Kennosuke
authors.initials: K
authors.lastname: Karube
authors.forename: Noriaki
authors.initials: N
authors.lastname: Yoshida
authors.forename: Toshiro
authors.initials: T
authors.lastname: Sugiyama
authors.forename: Masao
authors.initials: M
authors.lastname: Seto
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000473:pathology*; D019938:Cyclin D1 / Q000235:genetics; D004195:Disease Models, Animal; D019254:Genes, bcl-2; D016259:Genes, myc; D005822:Genetic Vectors; D066298:In Vitro Techniques; D008223:Lymphoma / Q000235:genetics* / Q000473:pathology; D051379:Mice; D012190:Retroviridae / Q000235:genetics*; D013601:T-Lymphocytes / Q000473:pathology*; D014161:Transduction, Genetic
chemical_list: C530105:Ccnd1 protein, mouse; D019938:Cyclin D1