title: Identification and characterization of proteins isolated from microvesicles derived from human lung cancer pleural effusions.
abstract: Microvesicles (MVs, also known as exosomes, ectosomes, microparticles) are released by various cancer cells, including lung, colorectal, and prostate carcinoma cells. MVs released from tumor cells and other sources accumulate in the circulation and in pleural effusion. Although recent studies have shown that MVs play multiple roles in tumor progression, the potential pathological roles of MV in pleural effusion, and their protein composition, are still unknown. In this study, we report the first global proteomic analysis of highly purified MVs derived from human nonsmall cell lung cancer (NSCLC) pleural effusion. Using nano-LC-MS/MS following 1D SDS-PAGE separation, we identified a total of 912 MV proteins with high confidence. Three independent experiments on three patients showed that MV proteins from PE were distinct from MV obtained from other malignancies. Bioinformatics analyses of the MS data identified pathologically relevant proteins and potential diagnostic makers for NSCLC, including lung-enriched surface antigens and proteins related to epidermal growth factor receptor signaling. These findings provide new insight into the diverse functions of MVs in cancer progression and will aid in the development of novel diagnostic tools for NSCLC.
authors.affiliation: Department of Molecular Biotechnology, WCU Program, Konkuk University, Seoul, Republic of Korea.
authors.forename: Jung Ok
authors.initials: JO
authors.lastname: Park
authors.forename: Do-Young
authors.initials: DY
authors.lastname: Choi
authors.forename: Dong-Sic
authors.initials: DS
authors.lastname: Choi
authors.forename: Hee Joung
authors.initials: HJ
authors.lastname: Kim
authors.forename: Jeong Won
authors.initials: JW
authors.lastname: Kang
authors.forename: Jae Hun
authors.initials: JH
authors.lastname: Jung
authors.forename: Jeong Hwa
authors.initials: JH
authors.lastname: Lee
authors.forename: Jayoung
authors.initials: J
authors.lastname: Kim
authors.forename: Michael R
authors.initials: MR
authors.lastname: Freeman
authors.forename: Kye Young
authors.initials: KY
authors.lastname: Lee
authors.forename: Yong Song
authors.initials: YS
authors.lastname: Gho
authors.forename: Kwang Pyo
authors.initials: KP
authors.lastname: Kim
mesh_terms: D000328:Adult; D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000737:chemistry* / Q000378:metabolism; D002853:Chromatography, Liquid; D030562:Databases, Protein; D066246:ErbB Receptors / Q000378:metabolism; D055354:Exosomes / Q000737:chemistry* / Q000378:metabolism; D005260:Female; D006801:Humans; D008168:Lung / Q000737:chemistry; D008175:Lung Neoplasms / Q000737:chemistry* / Q000378:metabolism; D008297:Male; D008854:Microscopy, Electron; D009363:Neoplasm Proteins / Q000032:analysis* / Q000737:chemistry / Q000145:classification; D010996:Pleural Effusion / Q000378:metabolism*; D020543:Proteome / Q000032:analysis / Q000737:chemistry; D040901:Proteomics; D015203:Reproducibility of Results; D015398:Signal Transduction; D053719:Tandem Mass Spectrometry
chemical_list: D009363:Neoplasm Proteins; D020543:Proteome; C512478:EGFR protein, human; D066246:ErbB Receptors
keywords: Cell biology; Exosome; Microvesicle; Nonsmall cell lung cancer; Pleural effusion