title: [Prediction and identification of Taenia solium oncosphere TSO45-4B antigen FnIII structure domain linear B cell epitopes].
abstract: OBJECTIVE
  To predict and identify Taenia solium oncosphere TSO45-4B antigen Fn III structure domain linear B cell epitopes.
  
  
  METHODS
  The B cell epitopes of TSO45-4B Fn III structure domain were predicted through the sequence analysis by using bioinformatics online tools and the protein space conformation was predicted by SWISS-MODEL. The peptides were synthesized according to the predicted linear epitopes. The immunoreactivity of sera of cysticercosis patients to the peptides synthesized was tested by using ELISA.
  
  
  RESULTS
  Two linear B cell epitopes of TSO45-4B Fn III structure domain were predicted, and one of the predicted epitope peptides synthesized could be recognized by the sera of cysticercosis patients.
  
  
  CONCLUSIONS
  Two linear B cell epitopes of TSO45-4B Fn III structure domain are predicted and one of them has been confirmed successfully.
authors.affiliation: Department of Microbiology and Parasitology, Bengbu Medical College, Anhui Key Laboratory of Infection and Immunity, Bengbu 233030, China.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Wang
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Wang
authors.forename: Xue-Lian
authors.initials: XL
authors.lastname: Chang
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Tao
authors.forename: Hui
authors.initials: H
authors.lastname: Xia
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fang
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000737:chemistry* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D018985:Epitopes, B-Lymphocyte / Q000737:chemistry* / Q000276:immunology; D005353:Fibronectins / Q000737:chemistry* / Q000276:immunology; D006801:Humans; D011487:Protein Conformation; D041201:Taenia solium / Q000737:chemistry* / Q000276:immunology
chemical_list: D000947:Antigens, Helminth; D018985:Epitopes, B-Lymphocyte; D005353:Fibronectins; C113407:TSOL-18 antigen