title: Antibacterial efficacy of prophylactic ozone treatment on patients with fixed orthodontic appliances.
abstract: OBJECTIVE
  This study investigated the efficacy of ozone on microflora of patients with fixed orthodontic appliances.
  
  
  METHODS
  Ten subjects undergoing fixed appliance therapy were enrolled in the study. Before any application, two elastic ligatures were removed with the aid of a sterile probe from the premolar brackets on the upper right and lower left jaws for baseline bacteriological counts. Then ozone gas was applied for 180 s by running the device on prophylaxis mode with included mouth tray according to the manufacturer's recommendations on all brackets. After the ozone application, two other elastic ligatures were removed from the opposite sides of the mouth of the patients. One week after the application two more elastic samples, which were not changed in the previous session, were collected from the upper right and lower left premolar brackets. The elastic ligatures were immersed in transfer solutions and cultured to determine the Streptococcus mutans and Lactobacillus acidophilus. Statistical analysis of the data was obtained by paired Student's t-tests.
  
  
  RESULTS
  The ozone treatment reduced the S. mutans and L. acidophilus immediately after the application and these reductions were found to be statistically significant (p = 0.038 and p = 0.020). Both the S. mutans and L. acidophilus values increased by the 1-week period and the S. mutans values were significantly higher than the baseline values (p = 0.01).
  
  
  CONCLUSIONS
  It can be concluded that the ozone treatment may have an instantaneous lethal effect on S. mutans and L. acidophilus; however, within the limitations of this study, a long-term preventive effect could not be observed.
authors.affiliation: Department of Pedodontics, Faculty of Dentistry.
authors.forename: Arzu
authors.initials: A
authors.lastname: Aykut-Yetkiner
authors.forename: Ece
authors.initials: E
authors.lastname: Eden
authors.forename: Fahinur
authors.initials: F
authors.lastname: Ertuğrul
authors.forename: Ecem
authors.initials: E
authors.lastname: Ergin
authors.forename: Mustafa
authors.initials: M
authors.lastname: Ateş
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D005260:Female; D006801:Humans; D008297:Male; D009967:Orthodontic Appliances; D010126:Ozone / Q000627:therapeutic use*; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents; D010126:Ozone