title: δ-Opioid receptor activation modified microRNA expression in the rat kidney under prolonged hypoxia.
abstract: Hypoxic/ischemic injury to kidney is a frequently encountered clinical problem with limited therapeutic options. Since microRNAs are differentially involved in hypoxic/ischemic events and δ-opioid receptor (DOR) activation is known to protect against hypoxic/ischemic injury, we speculated on the involvement of DOR activation in altering the microRNA (miRNA) expression in kidney under hypoxic condition. We selected 31 miRNAs based on microarray data for quantitative PCR analysis. Among them, 14 miRNAs were significantly altered after prolonged hypoxia, DOR activation or a combination of both. We found that 1) DOR activation alters miRNA expression profiles in normoxic conditions; 2) hypoxia differentially alters miRNA expression depending on the duration of hypoxia; and 3) DOR activation can modify hypoxia-induced changes in miRNA expression. For example, 10-day hypoxia reduced the level of miR-212 by over 70%, while DOR activation could mimic such reduction even in normoxic kidney. In contrast, the same stress increased miR-29a by >100%, which was reversed following DOR activation. These first data suggest that hypoxia comprehensively modifies the miRNA profile within the kidney, which can be mimicked or modified by DOR activation. Ascertaining the targeted pathways that regulate the diverse cellular and molecular functions of miRNA may provide new insights into potential therapies for hypoxic/ischemic injury of the kidney.
authors.affiliation: Research Institute of Modern Medicine, The Third Clinical College of Soochow University, Changzhou, Jiangsu, People's Republic of China.
authors.forename: Xiaozhou
authors.initials: X
authors.lastname: He
authors.forename: Yilin
authors.initials: Y
authors.lastname: Yang
authors.forename: Feng
authors.initials: F
authors.lastname: Zhi
authors.forename: Meredith L
authors.initials: ML
authors.lastname: Moore
authors.forename: Xuezhi
authors.initials: X
authors.lastname: Kang
authors.forename: Dongman
authors.initials: D
authors.lastname: Chao
authors.forename: Rong
authors.initials: R
authors.lastname: Wang
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Balboni
authors.forename: Severo
authors.initials: S
authors.lastname: Salvadori
authors.forename: Dong H
authors.initials: DH
authors.lastname: Kim
authors.forename: Ying
authors.initials: Y
authors.lastname: Xia
mesh_terms: D000818:Animals; D001835:Body Weight; D016000:Cluster Analysis; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D000860:Hypoxia; D007668:Kidney / Q000033:anatomy & histology / Q000378:metabolism*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D009929:Organ Size; D051381:Rats; D017465:Receptors, Opioid, delta / Q000378:metabolism*
chemical_list: D035683:MicroRNAs; D017465:Receptors, Opioid, delta