title: Evidence of small modulation of ethinylestradiol induced effects by concurrent exposure to trenbolone in male eelpout Zoarces viviparus.
abstract: The interaction of xenobiotics is common in aquatic ecosystems; therefore, we wanted to evaluate if trenbolone (TB) modulates the effects of 17α-ethinylestradiol (EE2). Male eelpout (Zoarces viviparus) were exposed to 5 ng L(-1) EE2 continuously for 19 d (EE2-C) or discontinuously (11 d, EE2-D) alone or in combination with low (50 ng L(-1), TBL) or high (500 ng L(-1), TBH) concentrations of TB (19 d). Exposure to EE2 caused reduced gonadosomatic index, increased plasma vitellogenin concentrations, up-regulated vtg and era mRNA expression and severe alterations in gonadal histology. TBL and TBH did not affect plasma vitellogenin, era or vtg mRNA expression. TBL and TBH did not counteract the EE2-induced increase in plasma vitellogenin and reduction in 11-ketotestosterone whereas TBH counteracted the EE2 induced increase in vtg and era mRNA expression. Exposure to TBH and EE2-C + TBH lead to severe gonadal histology alterations. TBL and EE2-D + TBH exposed fish showed less histopathological alterations.
authors.affiliation: Institute of Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark. ymvelascos@unillanos.edu.co
authors.forename: Yohana M
authors.initials: YM
authors.lastname: Velasco-Santamaría
authors.forename: Poul
authors.initials: P
authors.lastname: Bjerregaard
authors.forename: Bodil
authors.initials: B
authors.lastname: Korsgaard
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D004347:Drug Interactions; D052244:Endocrine Disruptors / Q000378:metabolism / Q000633:toxicity*; D004997:Ethinyl Estradiol / Q000378:metabolism / Q000633:toxicity*; D005399:Fishes / Q000502:physiology; D015870:Gene Expression / Q000187:drug effects; D008297:Male; D013091:Spermatogenesis / Q000187:drug effects; D013737:Testis / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D013739:Testosterone / Q000031:analogs & derivatives; D014204:Trenbolone Acetate / Q000378:metabolism / Q000633:toxicity*; D014819:Vitellogenins / Q000097:blood; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*
chemical_list: D015415:Biomarkers; D052244:Endocrine Disruptors; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; D013739:Testosterone; D004997:Ethinyl Estradiol; C003600:11-ketotestosterone; D014204:Trenbolone Acetate