title: A strategy to capture and characterize the synaptic transcriptome.
abstract: Here we describe a strategy designed to identify RNAs that are actively transported to synapses during learning. Our approach is based on the characterization of RNA transport complexes carried by molecular motor kinesin. Using this strategy in Aplysia, we have identified 5,657 unique sequences consisting of both coding and noncoding RNAs from the CNS. Several of these RNAs have key roles in the maintenance of synaptic function and growth. One of these RNAs, myosin heavy chain, is critical in presynaptic sensory neurons for the establishment of long-term facilitation, but not for its persistence.
authors.affiliation: Department of Neuroscience and Howard Hughes Medical Institute, Columbia University, New York, NY 10027, USA. sputhanv@scripps.edu
authors.forename: Sathyanarayanan V
authors.initials: SV
authors.lastname: Puthanveettil
authors.forename: Igor
authors.initials: I
authors.lastname: Antonov
authors.forename: Sergey
authors.initials: S
authors.lastname: Kalachikov
authors.forename: Priyamvada
authors.initials: P
authors.lastname: Rajasethupathy
authors.forename: Yun-Beom
authors.initials: YB
authors.lastname: Choi
authors.forename: Andrea B
authors.initials: AB
authors.lastname: Kohn
authors.forename: Mathew
authors.initials: M
authors.lastname: Citarella
authors.forename: Fahong
authors.initials: F
authors.lastname: Yu
authors.forename: Kevin A
authors.initials: KA
authors.lastname: Karl
authors.forename: Maxime
authors.initials: M
authors.lastname: Kinet
authors.forename: Irina
authors.initials: I
authors.lastname: Morozova
authors.forename: James J
authors.initials: JJ
authors.lastname: Russo
authors.forename: Jingyue
authors.initials: J
authors.lastname: Ju
authors.forename: Leonid L
authors.initials: LL
authors.lastname: Moroz
authors.forename: Eric R
authors.initials: ER
authors.lastname: Kandel
mesh_terms: D000818:Animals; D001048:Aplysia / Q000235:genetics*; D002490:Central Nervous System / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods*; D016678:Genome / Q000235:genetics; D059014:High-Throughput Nucleotide Sequencing; D017403:In Situ Hybridization; D016547:Kinesins / Q000378:metabolism; D017774:Long-Term Potentiation / Q000235:genetics; D018995:Myosin Heavy Chains / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D021381:Protein Transport / Q000235:genetics; D012313:RNA / Q000235:genetics / Q000378:metabolism; D034443:RNA Transport / Q000235:genetics; D017423:Sequence Analysis, RNA; D013569:Synapses / Q000235:genetics*; D059467:Transcriptome / Q000235:genetics*
chemical_list: D012313:RNA; D018995:Myosin Heavy Chains; D016547:Kinesins