title: A new human hepatoma cell line to study repeated cell toxicity.
abstract: Early toxicity screening of new drugs is performed to select candidates for development. Many cell models are used to assess basic cytotoxicity and to show a good correlation with acute toxicity. However, their correlation with chronic in vivo exposure is inadequate. The new hepatoma cell line (HBG BC2) possesses the capacities of being reversibly differentiated in vitro, and of maintaining a relatively higher metabolic rate when in the differentiated phase (3 weeks) as compared to Hep G2 cells. MTT reduction was used to evaluate the toxicity of propranolol, perhexiline, aspirin and paracetamol, after both single and repeated treatments (three times a week for 2 weeks). Under conditions of repeated treatment, cytotoxicity was observed at lower doses when compared with single administration. Moreover, the first non-toxic doses were in the same range as plasma concentrations measured in humans during therapeutic use. Our results suggest that the new human hepatoma HBG BC2 cell line may be of interest for the evaluation of cell toxicity under repeated treatment conditions.
authors.affiliation: Sanofi-Synthelabo Recherche, Toxicology-Porcheville, Genetic and Exploratory Toxicology Group, 2-8 Route de Rouen, 78440 Porcheville, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Fabre
authors.forename: Elsa
authors.initials: E
authors.lastname: Arrivet
authors.forename: Françoise
authors.initials: F
authors.lastname: Paillard
authors.forename: Valérie
authors.initials: V
authors.lastname: Wibaut-Berlaimont
authors.forename: Nicole
authors.initials: N
authors.lastname: Bichet
authors.forename: Nigel O
authors.initials: NO
authors.lastname: Roome
authors.forename: Annick
authors.initials: A
authors.lastname: Prenez
authors.forename: Joan-Albert
authors.initials: JA
authors.lastname: Vericat
mesh_terms: D001483:Base Sequence; D001711:Biotransformation; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D017931:DNA Primers; D015870:Gene Expression; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D018675:Toxicity Tests
chemical_list: D017931:DNA Primers