title: Nitric oxide production by Peromyscus yucatanicus (Rodentia) infected with Leishmania (Leishmania) mexicana.
abstract: Peromyscus yucatanicus (Rodentia: Cricetidae) is a primary reservoir of Leishmania (Leishmania) mexicana (Kinetoplastida: Trypanosomatidae). Nitric oxide (NO) generally plays a crucial role in the containment and elimination of Leishmania. The aim of this study was to determine the amount of NO produced by P. yucatanicus infected with L. (L.) mexicana. Subclinical and clinical infections were established in P. yucatanicus through inoculation with 1 x 10 2 and 2.5 x 10 6 promastigotes, respectively. Peritoneal macrophages were cultured alone or co-cultured with lymphocytes with or without soluble Leishmania antigen. The level of NO production was determined using the Griess reaction. The amount of NO produced was significantly higher (p ≤ 0.0001) in co-cultured macrophages and lymphocytes than in macrophages cultured alone. No differences in NO production were found between P. yucatanicus with subclinical L. (L.) mexicana infections and animals with clinical infections. These results support the hypothesis that the immunological mechanisms of NO production in P. yucatanicus are similar to those described in mouse models of leishmaniasis and, despite NO production, P. yucatanicus is unable to clear the parasite infection.
authors.affiliation: Laboratorio de Inmunología, Centro de Investigaciones Regionales Dr Hideyo Noguchi, Universidad Autónoma de Yucatán, Yucatán, México. nalleli.cervera@uady.mx
authors.forename: Elsy Nalleli
authors.initials: EN
authors.lastname: Loría-Cervera
authors.forename: Erika Ivett
authors.initials: EI
authors.lastname: Sosa-Bibiano
authors.forename: Liliana Estefanía
authors.initials: LE
authors.lastname: Villanueva-Lizama
authors.forename: Nicole Raymonde
authors.initials: NR
authors.lastname: Van Wynsberghe
authors.forename: Silvia Beatriz
authors.initials: SB
authors.lastname: Canto-Lara
authors.forename: José Luis
authors.initials: JL
authors.lastname: Batún-Cutz
authors.forename: Fernando José
authors.initials: FJ
authors.lastname: Andrade-Narváez
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D007894:Leishmania mexicana / Q000276:immunology*; D016773:Leishmaniasis, Cutaneous / Q000276:immunology*; D017737:Macrophages, Peritoneal / Q000276:immunology / Q000469:parasitology*; D009569:Nitric Oxide / Q000096:biosynthesis*; D010542:Peromyscus / Q000378:metabolism* / Q000469:parasitology
chemical_list: D009569:Nitric Oxide