title: An anti-complementary polysaccharide from the roots of Bupleurum chinense.
abstract: Bioactivity-guided fractionation of hot-water extract from the roots of Bupleurum chinense DC led to the isolation of a homogeneous polysaccharide BC-PS1 composed of Gal, GalA, Glc, Ara and Man in the ratio of 1.6:1.1:1.8:1.7:1.0, along with trace of Rha, Fuc and Xyl. The main linkages of the residues of BC-PS1 include terminal, 1,4-linked and 1,3-linked Galp (GalA), terminal and 1,4-linked Glcp, terminal and 1,5-linked Araf, 1,4,6-linked Manp. Bioassays revealed that the concentrations of BC-PS1 that resulted in 50% hemolytic inhibition on classical complement pathway (CH50) and alternative complement pathway (AP50) were 0.199±0.025 mg/mL and 0.371±0.017 mg/mL, respectively. Preliminary mechanism study indicated that BC-PS1 interacted with C1q, C2, C5 and C9.
authors.affiliation: School of Pharmacy, Fudan University, Shanghai 201203, People's Republic of China.
authors.forename: Hongye
authors.initials: H
authors.lastname: Di
authors.forename: Yunyi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Daofeng
authors.initials: D
authors.lastname: Chen
mesh_terms: D000328:Adult; D000818:Animals; D001777:Blood Coagulation / Q000187:drug effects; D029972:Bupleurum / Q000737:chemistry*; D002852:Chromatography, Ion Exchange; D003167:Complement Activation / Q000187:drug effects; D004353:Drug Evaluation, Preclinical; D004912:Erythrocytes / Q000187:drug effects; D006461:Hemolysis; D006801:Humans; D007155:Immunologic Factors / Q000302:isolation & purification / Q000494:pharmacology; D008297:Male; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry*; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D011817:Rabbits
chemical_list: D007155:Immunologic Factors; D010936:Plant Extracts; D011134:Polysaccharides