title: [Karyotype analysis using cells cultured from fetal bladder centesis].
abstract: OBJECTIVE
  To explore the feasibility of karyotype analysis using cells cultured from fetal bladder centesis samples.
  
  
  METHODS
  Samples were derived from fetal bladder centesis for 3 fetuses featuring giant bladder and oligohydramnios. Following in vitro culture, cells were routinely processed and stained for chromosome analysis.
  
  
  RESULTS
  For all 3 cases, cell culture has achieved success. Sufficient metaphase cells were obtained for chromosome counting and karyotype analysis. The karyotypes of the 3 fetuses were respectively 46, XY, 46, XX, t(1;5)(q22;q12)[7]/46, XX[4], and 46, XY.
  
  
  CONCLUSIONS
  Cells cultured from fetal bladder centesis may be used for karyotype analysis following in vitro culturing. This new approach can enable prenatal chromosome analysis for fetuses featuring smaller gestational weeks, giant bladder and oligohydramnios.
authors.affiliation: Hebei General Hospital, Shijiazhuang, P.R. China. gaojian8703@163.com
authors.forename: Jian
authors.initials: J
authors.lastname: Gao
authors.forename: Li-min
authors.initials: LM
authors.lastname: Rong
authors.forename: Bing
authors.initials: B
authors.lastname: Mei
authors.forename: Xiao-ping
authors.initials: XP
authors.lastname: Yu
authors.forename: Wen-chao
authors.initials: WC
authors.lastname: Guo
authors.forename: Yali
authors.initials: Y
authors.lastname: Li
mesh_terms: D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007621:Karyotyping; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D001743:Urinary Bladder / Q000002:abnormalities*