title: The cowpea trypsin inhibitor promoter drives expression in response to cellular maturation in Arabidopsis thaliana.
abstract: The bowman-birk type trypsin inhibitors accumulate in high concentration in legume and cereal seeds, especially during seed maturation and are considered to be involved in insect tolerance. The 5' flanking sequences of the trypsin inhibitor was isolated from cowpea genomic DNA using anchor PCR. Analysis of sequences showed presence of seed specific RY elements and also other elements associated with seed development such as abscisic acid responsive elements (ABA responsive elements; ABRE) and dehydration responsive elements (DRE). Spatial and temporal control of the promoter driven expression pattern was analyzed using gus as reporter. Expression was found to occur both in embryo and endosperm; starting from torpedo stage of embryogenesis and continuing till the stage of final maturation i.e. bent cotyledon stage. Additional expression analyses showed that the promoter actually drives expression in tissues like leaves, roots, stipules, etc., but followed a specific pattern. Comparative analysis of expression in seeds and other organs indicated that the promoter driven expression is in response to cellular maturation.
authors.affiliation: National Research Center on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi, 110 012 India.
authors.forename: S
authors.initials: S
authors.lastname: Anandhan
authors.forename: Insaf A
authors.initials: IA
authors.lastname: Qureshi
authors.forename: K R
authors.initials: KR
authors.lastname: Koundal
keywords: Bowman-Birk type Trypsin inhibitor; Cellular maturation; Regulation; Seed development; gus expression