title: Altered invertase activities of symptomatic tissues on Beet severe curly top virus (BSCTV) infected Arabidopsis thaliana.
abstract: Arabidopsis thaliana infected with Beet severe curly top virus (BSCTV) exhibits systemic symptoms such as stunting of plant growth, callus induction on shoot tips, and curling of leaves and shoot tips. The regulation of sucrose metabolism is essential for obtaining the energy required for viral replication and the development of symptoms in BSCTV-infected A. thaliana. We evaluated the changed transcript level and enzyme activity of invertases in the inflorescence stems of BSCTV-infected A. thaliana. These results were consistent with the increased pattern of ribulose-1,5-bisphosphate carboxylase/oxygenase activity and photosynthetic pigment concentration in virus-infected plants to supply more energy for BSCTV multiplication. The altered gene expression of invertases during symptom development was functionally correlated with the differential expression patterns of D-type cyclins, E2F isoforms, and invertase-related genes. Taken together, our results indicate that sucrose sensing by BSCTV infection may regulate the expression of sucrose metabolism and result in the subsequent development of viral symptoms in relation with activation of cell cycle regulation.
authors.affiliation: Department of Genetic Engineering, Sungkyunkwan University, Suwon, Korea.
authors.forename: Jungan
authors.initials: J
authors.lastname: Park
authors.forename: Soyeon
authors.initials: S
authors.lastname: Kim
authors.forename: Eunseok
authors.initials: E
authors.lastname: Choi
authors.forename: Chung-Kyun
authors.initials: CK
authors.lastname: Auh
authors.forename: Jong-Bum
authors.initials: JB
authors.lastname: Park
authors.forename: Dong-Giun
authors.initials: DG
authors.lastname: Kim
authors.forename: Young-Jae
authors.initials: YJ
authors.lastname: Chung
authors.forename: Taek-Kyun
authors.initials: TK
authors.lastname: Lee
authors.forename: Sukchan
authors.initials: S
authors.lastname: Lee
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics / Q000821:virology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D027461:Beta vulgaris / Q000821:virology; D002338:Carotenoids / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics; D002734:Chlorophyll / Q000378:metabolism; D016213:Cyclins / Q000235:genetics; D050684:E2F Transcription Factors / Q000235:genetics; D017791:Geminiviridae / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D005964:Glucosyltransferases / Q000378:metabolism; D056626:Inflorescence / Q000201:enzymology / Q000235:genetics / Q000821:virology; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics / Q000821:virology; D018547:Plant Stems / Q000201:enzymology / Q000235:genetics / Q000821:virology; D030821:Plants, Genetically Modified; D012273:Ribulose-Bisphosphate Carboxylase / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism; D043324:beta-Fructofuranosidase / Q000235:genetics* / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D018797:Cell Cycle Proteins; D016213:Cyclins; D050684:E2F Transcription Factors; D002734:Chlorophyll; D002338:Carotenoids; D013395:Sucrose; D005964:Glucosyltransferases; C022699:sucrose synthase; D043324:beta-Fructofuranosidase; D012273:Ribulose-Bisphosphate Carboxylase