title: Experimental infection of dogs with Leishmania and saliva as a model to study Canine Visceral Leishmaniasis.
abstract: BACKGROUND
  Canine Visceral Leishmaniasis (CVL) is a zoonotic disease caused by Leishmania infantum, transmitted by the bite of Lutzomyia longipalpis sand flies. Dogs are the main domestic reservoir of the parasite. The establishment of an experimental model that partially reproduces natural infection in dogs is very important to test vaccine candidates, mainly regarding those that use salivary proteins from the vector and new therapeutical approaches.
  
  
  RESULTS
  In this report, we describe an experimental infection in dogs, using intradermal injection of Leishmania infantum plus salivary gland homogenate (SGH) of Lutzomyia longipalpis. Thirty-five dogs were infected with 1×10(7) parasites combined with five pairs of Lutzomyia longipalpis salivary glands and followed for 450 days after infection and clinical, immunological and parasitological parameters were evaluated. Two hundred and ten days after infection we observed that 31,4% of dogs did not display detectable levels of anti-Leishmania antibodies but all presented different numbers of parasites in the lymph nodes. Animals with a positive xenodiagnosis had at least 3,35×10(5) parasites in their lymph nodes. An increase of IFN-γ and IL-10 levels was detected during infection. Twenty two percent of dogs developed symptoms of CVL during infection.
  
  
  CONCLUSIONS
  The infection model described here shows some degree of similarity when compared with naturally infected dogs opening new perspectives for the study of CVL using an experimental model that employs the combination of parasites and sand fly saliva both present during natural transmission.
authors.affiliation: Centro de Pesquisa Gonçalo Moniz, FIOCRUZ-BA, Bahia, Brazil.
authors.forename: Dirceu Joaquim
authors.initials: DJ
authors.lastname: Costa
authors.forename: Rayssa M de Araujo
authors.initials: RM
authors.lastname: Carvalho
authors.forename: Melissa
authors.initials: M
authors.lastname: Abbehusen
authors.forename: Clarissa
authors.initials: C
authors.lastname: Teixeira
authors.forename: Maiana
authors.initials: M
authors.lastname: Pitombo
authors.forename: Joelma
authors.initials: J
authors.lastname: Trigo
authors.forename: Flávia
authors.initials: F
authors.lastname: Nascimento
authors.forename: Lucilene
authors.initials: L
authors.lastname: Amorim
authors.forename: Ana Lucia
authors.initials: AL
authors.lastname: Abreu-Silva
authors.forename: Maria
authors.initials: M
authors.lastname: do Socorro Pires Cruz
authors.forename: José Carlos
authors.initials: JC
authors.lastname: Miranda
authors.forename: Kyoshi
authors.initials: K
authors.lastname: Fukutani
authors.forename: Camila I
authors.initials: CI
authors.lastname: de Oliveira
authors.forename: Aldina
authors.initials: A
authors.lastname: Barral
authors.forename: Manoel
authors.initials: M
authors.lastname: Barral-Netto
authors.forename: Cláudia
authors.initials: C
authors.lastname: Brodskyn
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D016207:Cytokines / Q000097:blood; D004195:Disease Models, Animal; D004283:Dog Diseases / Q000097:blood / Q000469:parasitology* / Q000635:transmission; D004285:Dogs; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D018314:Leishmania infantum / Q000276:immunology / Q000502:physiology*; D007898:Leishmaniasis, Visceral / Q000097:blood / Q000469:parasitology / Q000635:transmission / Q000662:veterinary*; D059208:Parasite Load; D011576:Psychodidae / Q000469:parasitology; D012463:Saliva / Q000469:parasitology*; D012469:Salivary Glands / Q000469:parasitology
chemical_list: D000913:Antibodies, Protozoan; D016207:Cytokines; D007074:Immunoglobulin G