title: Comparative analyses of laccase-catalyzed amination reactions for production of novel β-lactam antibiotics.
abstract: Seven novel β-lactam antibiotics with activities against Gram-positive bacterial strains, among them methicillin-resistant Staphylococcus aureus and vancomycin-resistant enterococci, were synthesized by amination of 2,5-dihydroxyphenylacetic acid in usable yields (30-60%). These products protected mice against an infection with S. aureus lethal to the control animals. The results show the usefulness of laccase for the synthesis of potential new antibiotics, in addition to the interdependence of the laccase substrates, the amino coupling partners, and the product formation, yield, and activity. The syntheses of β-lactam antibiotics with 2,5-dihydroxyaromatic acid substructures (para-substituted) are then compared with those of 3,4-dihydroxyaromatic acid substructures (ortho-substituted). Para-substituted laccase substrates were better reaction partners in these syntheses than ortho-substituted compounds.
authors.affiliation: Institute of Microbiology, Ernst-Moritz-Arndt-University Greifswald, Greifswald, Germany. annett.mikolasch@uni-greifswald.de
authors.forename: Annett
authors.initials: A
authors.lastname: Mikolasch
authors.forename: Katrin
authors.initials: K
authors.lastname: Manda
authors.forename: Rabea
authors.initials: R
authors.lastname: Schlüter
authors.forename: Michael
authors.initials: M
authors.lastname: Lalk
authors.forename: Sabine
authors.initials: S
authors.lastname: Witt
authors.forename: Simone
authors.initials: S
authors.lastname: Seefeldt
authors.forename: Elke
authors.initials: E
authors.lastname: Hammer
authors.forename: Frieder
authors.initials: F
authors.lastname: Schauer
authors.forename: Wolf-Dieter
authors.initials: WD
authors.lastname: Jülich
authors.forename: Ulrike
authors.initials: U
authors.lastname: Lindequist
mesh_terms: D000586:Amination; D000818:Animals; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis* / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D001554:Benzene / Q000737:chemistry / Q000378:metabolism; D055162:Biocatalysis; D001711:Biotransformation; D005260:Female; D042845:Laccase / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D020037:Sordariales / Q000201:enzymology; D047090:beta-Lactams / Q000138:chemical synthesis* / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D047090:beta-Lactams; D042845:Laccase; D001554:Benzene