title: Isolation of S-adenosyl-L-methionine synthetase gene from Panax ginseng C.A. meyer and analysis of its response to abiotic stresses.
abstract: A cDNA clone containing a S-adenosyl-L-methionine synthetase (SAMS) gene, named as PgSAM, was isolated from a commercial medicinal plant Panax ginseng. PgSAM is predicted to encode a precursor protein of 307 amino acid residues, and its sequence shares high homology with a number of other plant SAMS. PgSAM is expressed at different levels in various organs of ginseng. The expression of PgSAM in adventitious roots and hairy roots of P. ginseng were analyzed using reverse transcriptase (RT)-PCR and real-time PCR under various abiotic stresses. Salt, salicylic acid, abscisic acid and chilling stresses induced PgSAM significantly at different time points within 2-72 h post-treatment. This study revealed that PgSAM may help to protect the plants against various abiotic stresses.
authors.affiliation: Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University, 1 Seocheon, Giheung-gu Yongin-si, Gyeonggi-do, 449-701 South Korea.
authors.forename: Rama Krishna
authors.initials: RK
authors.lastname: Pulla
authors.forename: Yu-Jin
authors.initials: YJ
authors.lastname: Kim
authors.forename: Shohana
authors.initials: S
authors.lastname: Parvin
authors.forename: Ju-Sun
authors.initials: JS
authors.lastname: Shim
authors.forename: Jung-Hye
authors.initials: JH
authors.lastname: Lee
authors.forename: Yeon-Ju
authors.initials: YJ
authors.lastname: Kim
authors.forename: Jun-Gyo
authors.initials: JG
authors.lastname: In
authors.forename: Kalai Selvi
authors.initials: KS
authors.lastname: Senthil
authors.forename: Deok-Chun
authors.initials: DC
authors.lastname: Yang
keywords: Abiotic stress; Abscisic acid; Panax ginseng; S-Adenosyl-L-Methionine Synthetase (SAMS); Salicylic acid; Semi-quantitative RT-PCR; real-time PCR