title: Collagen modules for in situ delivery of mesenchymal stromal cell-derived endothelial cells for improved angiogenesis.
abstract: Modular tissue engineering is a strategy to create scalable, self-assembling, three-dimensional (3D) tissue constructs. This strategy was used to deliver endothelial-like cells derived from bone marrow mesenchymal stromal cells (EL-MSCs) to locally induce vascularization. First, tissue engineered modules were formed, comprising EL-MSCs and collagen-based cylinders. Seven days of module culture in a microfluidic chamber under continuous flow resulted in the formation of interstices, formed by random packing of the modules, which served as channels and were lined by the EL-MSCs. We observed maintenance of the endothelial phenotype of the EL-MSCs, as demonstrated by CD31 staining, and the cells proliferated well. Next, collagen modules covered with EL-MSCs, with or without embedded MSCs, were implanted subcutaneously in immune-compromised SCID/Bg mice. After 7 days, CD31-positive vessels were observed in the samples. These data demonstrate the feasibility of EL-MSCs coated collagen module as a strategy to locally stimulate angiogenesis and vasculogenesis. Copyright © 2013 John Wiley & Sons, Ltd.
authors.affiliation: MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, The Netherlands.
authors.forename: Karolina Janeczek
authors.initials: KJ
authors.lastname: Portalska
authors.affiliation: Institute of Biomaterials and Biomedical Engineering, University of Toronto, Ontario, Canada.
authors.forename: M Dean
authors.initials: MD
authors.lastname: Chamberlain
authors.affiliation: Institute of Biomaterials and Biomedical Engineering, University of Toronto, Ontario, Canada.
authors.forename: Chuen
authors.initials: C
authors.lastname: Lo
authors.affiliation: MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, The Netherlands.
authors.forename: Clemens
authors.initials: C
authors.lastname: van Blitterswijk
authors.affiliation: Institute of Biomaterials and Biomedical Engineering, University of Toronto, Ontario, Canada.
authors.forename: Michael V
authors.initials: MV
authors.lastname: Sefton
authors.affiliation: MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Enschede, The Netherlands.
authors.forename: Jan
authors.initials: J
authors.lastname: de Boer
mesh_terms: D000818:Animals; D002461:Cell Line, Transformed; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism / Q000637:transplantation; D064593:Heterografts; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D016513:Mice, SCID; D018919:Neovascularization, Physiologic
keywords: angiogenesis; collagen modules; endothelial differentiation; mesenchymal stromal cell; modular tissue engineering; vasculogenesis