title: A simple new competition assay for heparin binding in serum applied to multivalent PAMAM dendrimers.
abstract: We report a competition assay using our recently reported dye Mallard Blue, which allows us to identify synthetic heparin binders in competitive media, including human serum - using this we gain insight into the ability of PAMAM dendrimers to bind heparin, with the interesting result that low-generation G2-PAMAM is the preferred heparin binder.
authors.affiliation: Department of Chemistry, University of York, Heslington, York, YO10 5DD, UK.
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Bromfield
authors.forename: Paola
authors.initials: P
authors.lastname: Posocco
authors.forename: Maurizio
authors.initials: M
authors.lastname: Fermeglia
authors.forename: Sabrina
authors.initials: S
authors.lastname: Pricl
authors.forename: Julián
authors.initials: J
authors.lastname: Rodríguez-López
authors.forename: David K
authors.initials: DK
authors.lastname: Smith
mesh_terms: D001120:Arginine / Q000031:analogs & derivatives* / Q000737:chemistry; D004396:Coloring Agents / Q000737:chemistry*; D050091:Dendrimers / Q000737:chemistry* / Q000378:metabolism; D006493:Heparin / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D011485:Protein Binding; D044967:Serum / Q000737:chemistry; D013843:Thiazines / Q000737:chemistry*
chemical_list: D004396:Coloring Agents; D050091:Dendrimers; C104700:PAMAM Starburst; D013843:Thiazines; C584296:mallard blue; D006493:Heparin; D001120:Arginine