title: Porous collagen-hydroxyapatite scaffolds with mesenchymal stem cells for bone regeneration.
abstract: Current bone grafting materials have significant limitations for repairing maxillofacial and dentoalveolar bone deficiencies. An ideal bone tissue-engineering construct is still lacking. The purpose of the present study was first to synthesize and develop a collagen-hydroxyapatite (Col-HA) composite through controlled in situ mineralization on type I collagen fibrils with nanometer-sized apatite crystals, and then evaluate their biologic properties by culturing with mouse and human mesenchymal stem cells (MSCs). We synthesized Col-HA scaffolds with different Col:HA ratios. Mouse C3H10T1/2 MSCs and human periodontal ligament stem cells (hPDSCs) were cultured with scaffolds for cell proliferation and biocompatibility assays. We found that the porous Col-HA composites have good biocompatibility and biomimetic properties. The Col-HA composites with ratios 80:20 and 50:50 composites supported the attachments and proliferations of mouse MSCs and hPDSCs. These findings indicate that Col-HA composite complexes have strong potentials for bone tissue regeneration.
authors.affiliation: 1  University of Rochester Eastman Institute for Oral Health, Rochester, NY.
authors.forename: Li
authors.initials: L
authors.lastname: Ning
authors.forename: Hans
authors.initials: H
authors.lastname: Malmström
authors.forename: Yan-Fang
authors.initials: YF
authors.lastname: Ren
mesh_terms: D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry; D001861:Bone Regeneration / Q000502:physiology*; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques; D049109:Cell Proliferation / Q000502:physiology; D048430:Cell Shape; D011232:Chemical Precipitation; D024042:Collagen Type I / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry; D010513:Periodontal Ligament / Q000166:cytology; D016062:Porosity; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D024042:Collagen Type I; D017886:Durapatite
keywords: bone regeneration; collagen scaffolds; hydroxyapatite; mesenchymal stem cells; tissue engineering