title: Levels of supramolecular chirality of polyglutamine aggregates revealed by vibrational circular dichroism.
abstract: Polyglutamine (PolyQ) aggregates are a hallmark of several severe neurodegenerative diseases, expanded CAG-repeat diseases in which inheritance of an expanded polyQ sequence above a pathological threshold is associated with a high risk of disease. Application of vibrational circular dichroism (VCD) reveals that these PolyQ fibril aggregates exhibit a chiral supramolecular organization that is distinct from the supramolecular organization of previously observed amyloid fibrils. PolyQ fibrils grown from monomers with Q repeats 35 and above (Q≥35) exhibit approximately 10-fold enhancement of the same VCD spectrum compared to the already enhanced VCD of fibrils formed from Q repeats 30 and below (Q≤30).
authors.affiliation: University at Albany, State University of New York, Albany, NY 12222, USA.
authors.forename: Dmitry
authors.initials: D
authors.lastname: Kurouski
authors.forename: Karunakar
authors.initials: K
authors.lastname: Kar
authors.forename: Ronald
authors.initials: R
authors.lastname: Wetzel
authors.forename: Rina K
authors.initials: RK
authors.lastname: Dukor
authors.forename: Igor K
authors.initials: IK
authors.lastname: Lednev
authors.forename: Laurence A
authors.initials: LA
authors.lastname: Nafie
mesh_terms: D000682:Amyloid / Q000737:chemistry; D002942:Circular Dichroism; D006801:Humans; D007536:Isomerism; D007700:Kinetics; D010455:Peptides / Q000737:chemistry*; D055550:Protein Stability; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary
chemical_list: D000682:Amyloid; D010455:Peptides; C097188:polyglutamine