title: DNA-binding, photocleavage, cytotoxicity in vitro, apoptosis and cell cycle arrest studies of symmetric ruthenium(II) complexes.
abstract: Two novel ruthenium(II) complexes [Ru(dmb)2(addppn)](ClO4)2 (1) and [Ru(bpy)2(addppn)](ClO4)2 (2) were synthesized. The DNA-binding constants of complexes 1 and 2 were determined to be 4.78 (±0.49) × 10(5) and 7.42 (±0.53) × 10(5) M(-1). The results indicate that complexes 1 and 2 interact with CT DNA through intercalative mode. The cytotoxicity in vitro of the complexes toward BEL-7402, HeLa, MG-63 and SKBR-3 cells was assessed by MTT assay. The apoptosis was carried out with Hoechst 33258 staining method and flow cytometry. The cellular uptake was observed under fluorescence microscope. The cell cycle arrest shows that the antiproliferative mechanism induced by complexes 1 and 2 on BEL-7402 cells is G0/G1 phase arrest.
authors.affiliation: School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, PR China.
authors.forename: Yang-Yin
authors.initials: YY
authors.lastname: Xie
authors.forename: Hong-Liang
authors.initials: HL
authors.lastname: Huang
authors.forename: Jun-Hua
authors.initials: JH
authors.lastname: Yao
authors.forename: Gan-Jian
authors.initials: GJ
authors.lastname: Lin
authors.forename: Guang-Bin
authors.initials: GB
authors.lastname: Jiang
authors.forename: Yun-Jun
authors.initials: YJ
authors.lastname: Liu
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D059447:Cell Cycle Checkpoints / Q000187:drug effects*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D053837:DNA Cleavage / Q000187:drug effects / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D010777:Photochemistry; D012428:Ruthenium / Q000737:chemistry*; D013053:Spectrophotometry
chemical_list: D009942:Organometallic Compounds; D012428:Ruthenium; D004247:DNA