title: Comparative proteomics using lipid over-producing or less-producing mutants unravels lipid metabolisms in Chlamydomonas reinhardtii.
abstract: In this study, proteomic approach was employed to advance our understanding of genes associated with microalgal lipid production. We first generated random mutants of Chlamydomonas reinhardtii, which displayed increased or decreased lipid content, compared to that in the wild-type. Mutants were designated as CR12 and CR48. Then, the changes of protein expression associated with the mutations of Chlamydomonas reinhardtii were analyzed. We could identify proteins that were significantly up-regulated or down-regulated either in the wild-type or CR12 or CR48. Our results will help understand additional genes or pathways directly or indirectly linked to microalgal lipid production.
authors.affiliation: LED Agri-Bio Fusion Technology Research Center, Chonbuk National University, Iksan-si, Jeollabuk-do, Republic of Korea.
authors.forename: Yoon-E
authors.initials: YE
authors.lastname: Choi
authors.forename: Hyemin
authors.initials: H
authors.lastname: Hwang
authors.forename: Hyun-Soo
authors.initials: HS
authors.lastname: Kim
authors.forename: Joon-Woo
authors.initials: JW
authors.lastname: Ahn
authors.forename: Won-Joong
authors.initials: WJ
authors.lastname: Jeong
authors.forename: Ji-Won
authors.initials: JW
authors.lastname: Yang
mesh_terms: D016825:Chlamydomonas reinhardtii / Q000235:genetics* / Q000378:metabolism*; D002849:Chromatography, Gas; D015280:DNA Restriction-Modification Enzymes; D015180:Electrophoresis, Gel, Two-Dimensional; D018506:Gene Expression Regulation, Plant / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics; D007091:Image Processing, Computer-Assisted; D050356:Lipid Metabolism / Q000235:genetics*; D008055:Lipids / Q000096:biosynthesis*; D016296:Mutagenesis; D009154:Mutation / Q000235:genetics; D040901:Proteomics / Q000379:methods*; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D015280:DNA Restriction-Modification Enzymes; D008055:Lipids
keywords: Chlamydomonas reinhardtii; Comparative proteomics; Microalgal lipid biosynthesis; Random mutagenesis