title: [Effects of compound salvia miltiorrhiza injection on aquaporin 3 expression in human amniotic epithelial cells].
abstract: OBJECTIVE
  To study the effects of Compound Salvia miltiorrhiza Injection (CSI) on aquaporin 3 (AQP3) expression in human amniotic epithelial cells (hAECs), and to explore its mechanisms for treating oligohydramnios.
  
  
  METHODS
  The hAECs selected from 8 human term pregnancies with oligohydramnios and no other complications (as the test group)and 8 human term pregnancies with normal amniotic fluid volume (as the control group) were primarily cultured. The mRNA and protein expressions of AQP3 in hAECs were detected using reverse transcription-polymerase chain reaction and Western blot with various concentrations of CSI (0.000, 0.001, 0.010, 0.020, 0.060, and 0.100 mg/mL, respectively) at different time points (0, 6, 12,24, and 48 h, respectively).
  
  
  RESULTS
  (1) Compared with the control group, the AQP3 expression was down-regulated in the test group (P < 0.05). (2) The AQP3 expression in the two groups reached the peak when the concentration of CSI was 0.010 mg/mL, showing statistical difference when compared with other concentrations (P < 0.05). (3) The AQP3 expression reached the peak when 0.010 mg/mL CSI acted for 12 h, showing statistical difference when compared with other concentrations (P < 0.05). (4) The AQP3 expression was up-regulated in the two groups when 0.010 mg/mL CSI acted for 12 h. But the up-regulated AQP3 expression was more obvious in the test group than in the control group (P < 0.05).
  
  
  CONCLUSIONS
  CSI could regulate the AQP3 expression in hAECs. CSI showed more obvious effects on the AQP3 expression in hAECs of oligohydramnios human term pregnancies.
authors.affiliation: Department of Obstetrics and Gynecology, Second Affiliated Hospital of Wenzhou Medical College, Wenzhou 325027, China. zjwzzxq@163.com
authors.forename: Yi-Wen
authors.initials: YW
authors.lastname: Zhang
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Ma
authors.forename: Sheng-Di
authors.initials: SD
authors.lastname: Ding
authors.forename: Ai-Lan
authors.initials: AL
authors.lastname: Xie
authors.forename: Xue-Qiong
authors.initials: XQ
authors.lastname: Zhu
mesh_terms: D000650:Amnion / Q000166:cytology; D051400:Aquaporin 3 / Q000378:metabolism*; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D006801:Humans; D027561:Salvia miltiorrhiza
chemical_list: C496027:AQP3 protein, human; D004365:Drugs, Chinese Herbal; D051400:Aquaporin 3