title: A robust and efficient method for the isolation of DNA-free, pure and intact RNA from Mycobacterium tuberculosis.
abstract: We describe a robust method for the isolation of pure, intact RNA suitable for transcriptome studies from mycobacteria with consistent yields of 1 μg to 3 μg total RNA per 10(7) cells. The method reduces the use of hazardous chemicals and incorporates protocols for efficient removal of gDNA and rRNA.
authors.affiliation: Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021, India.
authors.forename: Balaji
authors.initials: B
authors.lastname: Venkataraman
authors.forename: Nidhi
authors.initials: N
authors.lastname: Gupta
authors.forename: Amita
authors.initials: A
authors.lastname: Gupta
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D020869:Gene Expression Profiling / Q000379:methods; D008967:Molecular Biology / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000235:genetics*; D012329:RNA, Bacterial / Q000302:isolation & purification*; D013048:Specimen Handling / Q000379:methods*
chemical_list: D012329:RNA, Bacterial