title: Photocytotoxicity of copper(II) complexes of curcumin and N-ferrocenylmethyl-L-amino acids.
abstract: Copper(II) complexes [Cu(Fc-aa)(cur)] (1-3) of curcumin (Hcur) and N-ferrocenylmethyl-L-amino acids (Fc-aa), viz., ferrocenylmethyl-L-tyrosine (Fc-TyrH), ferrocenylmethyl-L-tryptophan (Fc-TrpH) and ferrocenylmethyl-L-methionine (Fc-MetH), were prepared and characterized. The DNA photocleavage activity, photocytotoxicity and cellular localization in HeLa and MCF-7 cancer cells of these complexes were studied. Acetylacetonate (acac) complexes [Cu(Fc-aa)(acac)] (4-6) were prepared and used as controls. The chemical nuclease inactive complexes showed efficient pUC19 DNA cleavage activity in visible light. Complexes 1-3 showed high photocytotoxicity with low dark toxicity thus giving remarkable photodynamic effect. FACScan analysis showed apoptosis of the cancer cells. Fluorescence microscopic studies revealed primarily cytosolic localization of the complexes.
authors.affiliation: Department of Inorganic and Physical Chemistry, Indian Institute of Science, Sir C.V. Raman Avenue, Bangalore 560 012, India.
authors.forename: Tridib K
authors.initials: TK
authors.lastname: Goswami
authors.forename: Sudarshan
authors.initials: S
authors.lastname: Gadadhar
authors.forename: Bappaditya
authors.initials: B
authors.lastname: Gole
authors.forename: Anjali A
authors.initials: AA
authors.lastname: Karande
authors.forename: Akhil R
authors.initials: AR
authors.lastname: Chakravarty
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D003300:Copper / Q000737:chemistry*; D003474:Curcumin / Q000737:chemistry*; D053837:DNA Cleavage / Q000187:drug effects / Q000528:radiation effects; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D008027:Light; D061986:MCF-7 Cells; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D010777:Photochemistry
chemical_list: D000596:Amino Acids; D009942:Organometallic Compounds; D003300:Copper; D003474:Curcumin