title: Transplantation of bone-marrow-derived mesenchymal and epidermal stem cells contribute to wound healing with different regenerative features.
abstract: A new concept for wound therapy is the initiation of the regeneration of epidermal and dermal layers with appendages for skin function recovery. Bone-marrow-derived mesenchymal and epidermal stem cells (BMSCs and SSCs) are hypothesized to be able to home toward or to be transplanted to wound sites for skin repair and regeneration, but this awaits confirmation by further experimental and clinical evidence. In this study, the influence of the transplantation of BMSCs and SSCs with porous gelatin-β-tricalcium phosphate sponge as scaffolds on wound re-epithelization, collagen synthesis, skin tensile strength recovery, and skin appendage regeneration has been investigated. The transplantation of BMSCs or SSCs significantly accelerates wound re-epithelization, stimulates dermal collagen synthesis, and exhibits the trend to enhance the tensile strength recovery of skin. Furthermore, regenerative features of BMSCs and SSCs have been identified in activating blood vessel and hair follicle formation, respectively. These results not only provide experimental evidence for the application of BMSCs and SSCs as promising therapeutics for clinical wound treatment, but also display their characteristics in activating distinct skin appendage regeneration, which might have novel applications in skin tissue engineering.
authors.affiliation: Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, 866 Yuhangtang Road, 310058, Hangzhou City, People's Republic of China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Peng
authors.forename: Zu-Yao
authors.initials: ZY
authors.lastname: Mao
authors.forename: Xiao-Tian
authors.initials: XT
authors.lastname: Qi
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Ni
authors.initials: N
authors.lastname: Li
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Tabata
authors.forename: Jian-Qing
authors.initials: JQ
authors.lastname: Gao
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D002130:Calcium Phosphates / Q000494:pharmacology; D002469:Cell Separation; D048430:Cell Shape / Q000187:drug effects; D000078404:Epidermal Cells; D007150:Immunohistochemistry; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration / Q000502:physiology*; D013194:Staining and Labeling; D013718:Tensile Strength / Q000187:drug effects; D014945:Wound Healing / Q000187:drug effects
chemical_list: D002130:Calcium Phosphates; D019408:Platelet Endothelial Cell Adhesion Molecule-1; C018392:tricalcium phosphate