title: Preliminary pharmacological evaluation of Martynia annua Linn leaves for wound healing.
abstract: OBJECTIVE
  To evaluate the wound healing potential of fractions from ethanol extract of Martynia annua (M. annua) Linn leaves.
  
  
  METHODS
  Ethanol extract of M. annua Linn leaves was fractionate into three different fractions (MAF-A, MAF-B and MAF-C) which were screened for wound healing potential using two models: excision and incision on rats. The thin layer chromatography (TLC) profile of all fractions were analyzed and TLC of luteolin was also done. The Povidone-Iodine Ointment was used as reference for comparision. Excision and incision wounds were created on dorsal portion of rats for study. Wound contraction, biochemical parameters (protein level and hydroxyproline level) and histopathological study were performed in excision wound model whereas incision model was used for determination of tensile strength.
  
  
  RESULTS
  The wound contraction and tensile strength of skin tissues were observed significantly greater in MAF-C fraction treated group than other two fractions (P<0.01). In excision wound method (on day 18) protein content and hydroxyproline were found significantly higher in MAF-C group than control group (P<0.01). Histopathological study also showed better angiogenesis, matured collagen fibres and fibroblast cells as compared with the control group.
  
  
  CONCLUSIONS
  In conclusion, our findings suggest that fraction MAF-C from ethanol extract of M. annua leaves is found most effective in wound healing.
authors.affiliation: Department of Pharmaceutical Sciences, Dr. H. S. Gour University SAGAR (M.P.), India.
authors.forename: Lodhi
authors.initials: L
authors.lastname: Santram
authors.forename: A K
authors.initials: AK
authors.lastname: Singhai
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000302:isolation & purification / Q000627:therapeutic use*; D005260:Female; D006133:Growth Substances / Q000302:isolation & purification / Q000627:therapeutic use*; D008297:Male; D010936:Plant Extracts / Q000302:isolation & purification / Q000627:therapeutic use*; D018515:Plant Leaves / Q000737:chemistry; D017208:Rats, Wistar; D064028:Tracheophyta / Q000737:chemistry*; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000891:Anti-Infective Agents, Local; D006133:Growth Substances; D010936:Plant Extracts
keywords: Excision; Incision; Luteolin; Martynia annua; Povidone-iodine ointment; TLC; Tensile strength; Wound contraction; Wound healing