title: Effect of microformulation on the bioactivity of an anthocyanin-rich bilberry pomace extract ( Vaccinium myrtillus L.) in vitro.
abstract: In cell culture were compared the different release rates of anthocyanins from a bilberry pomace extract encapsulated either in food grade whey protein-based matrix capsules (WPC) or in pectin amid-based hollow spherical capsules (PHS). The impact of the formulations on typical anthocyanin-associated biological end points such as inhibition of the epidermal growth factor receptor (EGFR) and suppression of cell growth in HT29 colon carcinoma cells was assessed. The purpose was to find whether the release rates are sufficient to maintain biological activity and whether encapsulation affected EGFR inhibitory and growth suppressive properties of the extract. Even though anthocyanin release from extract-loaded capsules was proven under cell culture conditions, the inhibitory potential toward the EGFR was diminished. However, nonencapsulated extract as well as both extract-loaded encapsulation systems diminished the growth of HT29 cells to a comparable extent. The loss of EGFR inhibitory properties by encapsulation despite anthocyanin release indicates substantial contribution of other further constituents not monitored so far. Taken together, both applied encapsulation strategies allowed anthocyanin release and maintained biological activity with respect to growth inhibitory properties. However, the loss of EGFR inhibitory effects emphasizes the need for biological profiling to estimate process-induced changes of plant constituent's beneficial potencies.
authors.affiliation: Department of Food Chemistry and Toxicology, University of Vienna, Vienna, Austria.
authors.forename: Christopher
authors.initials: C
authors.lastname: Kropat
authors.forename: Michael
authors.initials: M
authors.lastname: Betz
authors.forename: Ulrich
authors.initials: U
authors.lastname: Kulozik
authors.forename: Sabine
authors.initials: S
authors.lastname: Leick
authors.forename: Heinz
authors.initials: H
authors.lastname: Rehage
authors.forename: Ute
authors.initials: U
authors.lastname: Boettler
authors.forename: Nicole
authors.initials: N
authors.lastname: Teller
authors.forename: Doris
authors.initials: D
authors.lastname: Marko
mesh_terms: D000872:Anthocyanins / Q000032:analysis*; D002214:Capsules; D004339:Drug Compounding / Q000009:adverse effects; D004355:Drug Stability; D066246:ErbB Receptors / Q000037:antagonists & inhibitors; D005638:Fruit / Q000737:chemistry*; D006131:Growth Inhibitors / Q000494:pharmacology; D019073:HT29 Cells / Q000737:chemistry / Q000187:drug effects / Q000473:pathology; D006801:Humans; D008894:Milk Proteins; D010368:Pectins; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D029800:Vaccinium myrtillus / Q000737:chemistry*; D000067816:Whey Proteins
chemical_list: D000872:Anthocyanins; D002214:Capsules; D006131:Growth Inhibitors; D008894:Milk Proteins; D010936:Plant Extracts; D000067816:Whey Proteins; D010368:Pectins; D066246:ErbB Receptors