title: [Application of a fluorescent dye method based on changes in membrane potential in detecting shellfish toxins].
abstract: OBJECTIVE
  To develop a rapid and sensitive assay for detecting the common sea food toxins including paralytic shellfish poisoning toxin (PST), tetrodotoxin (TTX) and neurotoxic shellfish poisoning toxin (NST) based on their toxicological character.
  
  
  METHODS
  Neuroblastoma cells were incubated with the fluorescent dye bis-oxonol, whose distribution across the membrane was potential-dependent. Changes in membrane potential of the cells induced by gonyautoxins (GTX2,3), brevetoxin (BTX) and TTX were observed respectively, using bis-oxonol.
  
  
  RESULTS
  Within 2 - 200 nmol/L of GTX2,3 or 20 - 600 nmol/L of TTX, veratridine-induced depolarization was shown to be inhibited by GTX2,3 or TTX in dose-dependent manner. Within 15 - 400 ng/ml, there was a dose-dependent relationship between the NSP-induced depolarization and toxin concentration.
  
  
  CONCLUSIONS
  It was likely to find a rapid, specific, and reliable method with bis-oxonol for detecting GTX2,3, TTX and BTX in sea food.
authors.affiliation: College of Life Sciences and Technology, Jinan University, Guangzhou 510632, China. gaoli_138@163.com
authors.forename: Lijuan
authors.initials: L
authors.lastname: Gao
authors.forename: Weidong
authors.initials: W
authors.lastname: Yang
authors.forename: Hongye
authors.initials: H
authors.lastname: Li
authors.forename: Jiesheng
authors.initials: J
authors.lastname: Liu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008387:Marine Toxins / Q000032:analysis*; D008564:Membrane Potentials / Q000187:drug effects*; D008974:Mollusca / Q000737:chemistry; D009447:Neuroblastoma / Q000473:pathology; D035964:Oxocins / Q000032:analysis; D012530:Saxitoxin / Q000031:analogs & derivatives* / Q000032:analysis; D012758:Shellfish / Q000032:analysis / Q000633:toxicity*; D013194:Staining and Labeling; D013779:Tetrodotoxin / Q000032:analysis; D000095702:Polyether Toxins
chemical_list: D005456:Fluorescent Dyes; D008387:Marine Toxins; D035964:Oxocins; D012530:Saxitoxin; D013779:Tetrodotoxin; C013007:gonyautoxins; C053342:brevetoxin; D000095702:Polyether Toxins