title: Identification of a new Newcastle disease virus isolate from Indonesia represents an ancestral lineage of class II genotype XIII.
abstract: An unknown virus was isolated from a mosquito pool collected in Jakarta during routine surveillance in 1979. Analysis of the sample using the Illumina platform resulted in the identification of a Newcastle disease virus (NDV) isolate. The sequence of the isolate indicated that it is an ancestral lineage of class II, genotype XIII. The source of the isolate is unusual, as newcastle disease virus is not believed to be vector-borne, although this mosquito pool was processed in a laboratory also handling samples for avian influenza surveillance and it is possible that this resulted in cross-contamination. This NDV isolate is still ancestral to most extant genotype XIII strains and provides a useful insight into historic NDV evolution.
authors.affiliation: Department of Pathology, Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, TX 77555-0609, USA. naforres@utmb.edu
authors.forename: Naomi L
authors.initials: NL
authors.lastname: Forrester
authors.forename: Steve G
authors.initials: SG
authors.lastname: Widen
authors.forename: Thomas G
authors.initials: TG
authors.lastname: Wood
authors.forename: Amelia P
authors.initials: AP
authors.lastname: Travassos da Rosa
authors.forename: Thomas G
authors.initials: TG
authors.lastname: Ksiazek
authors.forename: Nikos
authors.initials: N
authors.lastname: Vasilakis
authors.forename: Robert B
authors.initials: RB
authors.lastname: Tesh
mesh_terms: D000818:Animals; D002645:Chickens; D003465:Culex / Q000821:virology*; D005838:Genotype; D007214:Indonesia; D007303:Insect Vectors / Q000821:virology*; D008969:Molecular Sequence Data; D009521:Newcastle Disease / Q000635:transmission / Q000821:virology*; D009522:Newcastle disease virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D011201:Poultry Diseases / Q000635:transmission / Q000821:virology*