title: Consolidated bioprocessing of highly concentrated Jerusalem artichoke tubers for simultaneous saccharification and ethanol fermentation.
abstract: Consolidated bioprocessing (CBP) of Jerusalem artichoke tuber (Jat) for ethanol production is one of the most promising options for an alternate biofuel technology development. The technical barriers include the weak saccharolytic enzyme (inulinase) activity of the fermentation strain, and the well mixing of the high viscous fermentation slurry at the highly concentrated Jat loading. In this study, Saccharomyces cerevisiae DQ1 was found to produce relatively large amount of inulinase for hydrolysis of inulin in Jat, and the helical ribbon stirring bioreactor used provided well mixing performance under the high Jat loading. Even a highly concentrated Jat loading up to 35% (w/w) in the helical ribbon bioreactor for CBP was allowed. The results obtained from this study have demonstrated a feasibility of developing a CBP process technology in the helical ribbon bioreactor for ethanol production at a high yield 128.7 g/L and the theoretical yield 73.5%, respectively. This level of ethanol yield from Jat is relatively higher than others reported so far. The results of this study could provide a practical CBP process technology in the helical ribbon bioreactor for economically sustainable alternate biofuel production using highly concentrated inulin containing biomass feedstock such as Jat, at least 35%.
authors.affiliation: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
authors.forename: Lihao
authors.initials: L
authors.lastname: Guo
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Fengxian
authors.initials: F
authors.lastname: Hu
authors.forename: Dewey
authors.initials: D
authors.lastname: Dy Ryu
authors.forename: Jie
authors.initials: J
authors.lastname: Bao
mesh_terms: D018533:Biomass; D019149:Bioreactors; D004795:Enzyme Stability; D000431:Ethanol / Q000032:analysis / Q000378:metabolism*; D005285:Fermentation / Q000502:physiology*; D005632:Fructose / Q000032:analysis / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000032:analysis / Q000378:metabolism; D006368:Helianthus / Q000737:chemistry / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D042541:Intracellular Space / Q000378:metabolism; D035281:Plant Tubers / Q000737:chemistry / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000201:enzymology / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000032:analysis / Q000378:metabolism; D013696:Temperature
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D005632:Fructose; D000431:Ethanol; D006026:Glycoside Hydrolases; C023086:inulinase
keywords: Jerusalem artichoke tuber (Jat); Saccharomyces cerevisiae DQ1; consolidated bioprocessing (CBP); high titer ethanol; inulin; inulinase