title: Significance of the transient receptor potential canonical 2 (TRPC2) channel in the regulation of rat thyroid FRTL-5 cell proliferation, migration, adhesion and invasion.
abstract: Mammalian transient receptor potential (TRP) channels are involved in many physiologically important processes. Here, we have studied the significance of the TRPC2 channel in the regulation of rat thyroid FRTL-5 cell proliferation, migration, adhesion and invasion, using stable TRPC2 (shTRPC2) knock-down cells. In the shTRPC2 cells, proliferation was decreased due to a prolonged G1/S cell cycle phase. The tumor suppressor p53 and the cyclin-dependant kinase inhibitors p27 and p21 were upregulated. Cell invasion, adhesion and migration were also attenuated in shTRPC2 cells, probably due to decreased activity of both Rac and calpain, and a decreased secretion and activity of matrix metalloproteinase 2. The attenuated proliferation, migration, invasion and ATP-evoked calcium entry was mimicked by overexpressing a non-conducting, truncated TRPC2 (TRPC2-DN) in wild type cells, and was reversed by overexpression of TRPC2-GFP in shTRPC2 cells. In conclusion, TRPC2 is an important regulator of rat thyroid cell function.
authors.affiliation: Department of Biosciences, Åbo Akademi University, Tykistökatu 6A, 20520 Turku, Finland.
authors.forename: Pramod
authors.initials: P
authors.lastname: Sukumaran
authors.forename: Christoffer
authors.initials: C
authors.lastname: Löf
authors.forename: Ilari
authors.initials: I
authors.lastname: Pulli
authors.forename: Kati
authors.initials: K
authors.lastname: Kemppainen
authors.forename: Tero
authors.initials: T
authors.lastname: Viitanen
authors.forename: Kid
authors.initials: K
authors.lastname: Törnquist
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002154:Calpain / Q000235:genetics / Q000378:metabolism; D002448:Cell Adhesion; D002460:Cell Line; D002465:Cell Movement; D049109:Cell Proliferation; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000235:genetics / Q000378:metabolism; D059585:G1 Phase Cell Cycle Checkpoints; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D051381:Rats; D015398:Signal Transduction; D050052:TRPC Cation Channels / Q000235:genetics* / Q000378:metabolism; D013961:Thyroid Gland / Q000166:cytology / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism; D054462:p21-Activated Kinases / Q000235:genetics / Q000378:metabolism
chemical_list: D034741:RNA, Small Interfering; D050052:TRPC Cation Channels; C491857:Trpc2 protein, rat; D016159:Tumor Suppressor Protein p53; D050760:Cyclin-Dependent Kinase Inhibitor p27; D000255:Adenosine Triphosphate; D051057:Proto-Oncogene Proteins c-akt; D054462:p21-Activated Kinases; D002154:Calpain; D020778:Matrix Metalloproteinase 2; D002118:Calcium