title: [Effects end mechanisms of curcumol beta-cyclodextrin compound on the proliferation and apoptosls of esophageal carcinoma cell line TE-1].
abstract: OBJECTIVE
  To investigate the effects and mechanisms of Curcumol beta-cyclodextrin Compound (CbetaC) on the proliferation and apoptosis of esophageal carcinoma cell line TE-1.
  
  
  METHODS
  The CbetaC was prepared by saturated solution and confirmed by infrared absorption spectroscopy. The effects of CbetaC (at 25, 50, 100 mg/L) on the proliferation of human esophageal carcinoma cell line TE-1 in vitro was analyzed by MTT assay. The cell cycles and apoptosis were detected by flow cytometer. The relative expression of survivin mRNA was detected by real-time fluorescent quantitative PCR and calculated by the 2(-deltaCt) method. The protein expression of survivin was measured by Western blot.
  
  
  RESULTS
  Compared with the control group, results of MTT showed that CbetaC at each dose significantly inhibited the proliferation of TE-1 cells in a dose-dependent manner (P < 0.05). The results of flow cytometry showed that CbetaC resulted in the cell cycle arrest at G0/G1 and G2/M phase, and promoted the cell apoptosis. Besides, when compared with the control group, the protein and mRNA expressions of survivin obviously decreased in each CbetaC group (P < 0.05).
  
  
  CONCLUSIONS
  CbetaC could inhibit the proliferation of esophageal carcinoma cell TE-1 and promote the apoptosis. Its inhibition on the survivin expression was correlated with its inhibition on the malignant phenotypes of esophageal carcinoma cells.
authors.affiliation: Department of Chemoradiation, First Affiliated Hospital of Wenzhou Medical College, Zhejiang 325000, China. jz96329@163.com
authors.forename: Zhao
authors.initials: Z
authors.lastname: Jing
authors.forename: Cong-Ying
authors.initials: CY
authors.lastname: Xie
authors.forename: Zhi-Qin
authors.initials: ZQ
authors.lastname: Wu
authors.forename: Fang
authors.initials: F
authors.lastname: Xu
authors.forename: Chang-Lin
authors.initials: CL
authors.lastname: Zou
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004938:Esophageal Neoplasms / Q000473:pathology; D006801:Humans; D012717:Sesquiterpenes / Q000494:pharmacology*; D047392:beta-Cyclodextrins / Q000494:pharmacology
chemical_list: D012717:Sesquiterpenes; D047392:beta-Cyclodextrins; C022801:curcumol; C031215:betadex