title: A novel method of silkworm embryo preparation for immunohistochemistry.
abstract: It is difficult to obtain intact embryos, especially intact early embryos, from insect eggs because of their small sizes. Based on the means traditionally used to get silkworm embryos and the previous approaches used for getting Drosophila embryos, we established a novel method of silkworm embryo preparation. The new method is straightforward and easy to operate. Silkworm embryos could be prepared without severe damage in large quantities by this new protocol. In addition, the novel method of silkworm embryo preparation is quite suitable for immunohistochemistry.
authors.affiliation: Institute of Life Sciences, JiangSu University, Zhenjiang, 212013, Jiangsu, China. youzaizhi@126.com
authors.forename: Zaizhi
authors.initials: Z
authors.lastname: You
authors.forename: Chunfeng
authors.initials: C
authors.lastname: Sun
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.forename: Qin
authors.initials: Q
authors.lastname: Yao
authors.forename: Keping
authors.initials: K
authors.lastname: Chen
mesh_terms: D000818:Animals; D012831:Bombyx / Q000196:embryology*; D004772:Entomology / Q000379:methods*; D007150:Immunohistochemistry / Q000379:methods*