title: Two new steroidal saponins from Selaginella uncinata (Desv.) Spring and their protective effect against anoxia.
abstract: Four steroidal saponins were isolated from the anti-anoxic fraction of the 60% EtOH extract of Selaginella uncinata, including two new compounds, (3β, 7β, 12β, 25R)-spirost-5-ene-3, 7, 12-triol-3-O-α-L-rhamnopyranosyl-(1→2)-O-[α-L-rhamnopyranosyl-(1→4)]-O-β-D-glucopyranoside (1), (2α, 3β, 12β, 25R)-spirost-5-ene-2, 3, 12-triol-3-O-α-L-rhamnopyranosyl-(1→2)-O-[α-L-rhamnopyranosyl-(1→4)]-O-β-D-glucopyranoside (2) and two known compounds, (3β, 12β, 25R)-spirost-5-ene-3,12-diol-3-O-α-L-rhamnopyranosyl-(1→2)-O-[α-L-rhamnopyranosyl-(1→4)]-O-β-D-glucopyranoside, (3), (1α, 3β, 25R)-spirost-5-ene-2-diol-3-O-α-L-rhamnopyranosyl-(1→2)-O-[α-L-rhamnopyranosyl(1→4)]-O-β-D-glucopyranoside (4). The four compounds showed potent protective effect against anoxia in the anoxic PC12 cells assay, among which compounds 1 and 2 were the most active. To our knowledge, this is the first study to report the steroidal saponins in the plant S. uncinata and demonstrate their protective effect against anoxia in PC12 cell assay.
authors.affiliation: Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, PR China. Electronic address: junxiazheng@163.com.
authors.forename: Junxia
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhi
authors.affiliation: Institute of Traditional Chinese Medicine and Natural Products, Jinan University, Guangzhou 510632, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Dai
authors.affiliation: Key Lab for Research & Development of New Drugs, Research Institute of Tsinghua University in Shenzhen, Shenzhen 518057, PR China.
authors.forename: Naili
authors.initials: N
authors.lastname: Wang
authors.affiliation: Institute of Basic Medical Sciences, Beijing 100850, PR China.
authors.forename: Liying
authors.initials: L
authors.lastname: Wu
authors.affiliation: Institute of Basic Medical Sciences, Beijing 100850, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Fan
authors.affiliation: Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, PR China.
authors.forename: Yanxiong
authors.initials: Y
authors.lastname: Fang
authors.affiliation: Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, PR China.
authors.forename: Suqing
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, PR China.
authors.forename: Kun
authors.initials: K
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000860:Hypoxia / Q000517:prevention & control*; D016716:PC12 Cells; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D012503:Saponins / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D032503:Selaginellaceae / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D012503:Saponins
keywords: Protective effect against anoxia; Selaginella uncinata; Steroidal saponins