title: Development and validation studies for determination of phenylpropanoid-substituted flavan-3-ols in semipurified extract of Trichilia catigua by high-performance liquid chromatography with photodiode array detection.
abstract: The phenolic compounds are the main phytochemical constituents of the bark of Trichilia catigua and are commonly used for medicinal purposes. An HPLC method for the simultaneous quantification of phenolic compounds (procyanidin B2 (PB2), epicatechin (EPC), chinchonains Ia, Ib, IIa, IIb, catechin, and chrologenic acid) in T. catigua extract was developed and validated. A suitable chromatographic system was selected, which uses a gradient elution with methanol/ACN (75:25), and water both with 0.05% TFA, as mobile phase, column Luna, 280 nm, and flow 0.4 mL/min. Validation of the analytical method was based on the parameters: linearity, precision, LODs and LOQs, accuracy, robustness, and stability. The method showed linearity for PB2 and EPC, in the range 10-120 μg/mL with good correlation coefficients (>0.996). For precision, the repeatability ranged from 1.89 to 3.23%, and the values for accuracy for PB2 and EPC were 95 and 89%, respectively. The LODs and LOQs for PB2 were 1.36 and 4.12 μg/mL, and for EPC were 2.18 and 6.61 μg/mL, respectively. The method was robust under the conditions employed. The proposed method could be employed for quality assessment of T. catigua, as well as pharmaceutical products.
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Department of Pharmacy, Universidade Estadual de Maringá, Maringá, PR, Brazil.
authors.forename: Renata
authors.initials: R
authors.lastname: Longhini
authors.forename: Traudi
authors.initials: T
authors.lastname: Klein
authors.forename: Marcos Luciano
authors.initials: ML
authors.lastname: Bruschi
authors.forename: Waldir Veríssimo
authors.initials: WV
authors.lastname: da Silva
authors.forename: Juliana
authors.initials: J
authors.lastname: Rodrigues
authors.forename: Norberto Peporine
authors.initials: NP
authors.lastname: Lopes
authors.forename: João Carlos Palazzo
authors.initials: JC
authors.lastname: de Mello
mesh_terms: D044946:Biflavonoids / Q000032:analysis; D002392:Catechin / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D044950:Flavanones / Q000737:chemistry*; D054023:Lasers, Semiconductor; D057230:Limit of Detection; D029582:Meliaceae / Q000737:chemistry*; D015394:Molecular Structure; D028321:Plant Preparations / Q000737:chemistry*; D059808:Polyphenols / Q000737:chemistry*; D044945:Proanthocyanidins / Q000032:analysis
chemical_list: D044946:Biflavonoids; D044950:Flavanones; D028321:Plant Preparations; D059808:Polyphenols; D044945:Proanthocyanidins; C017674:procyanidin; D002392:Catechin