title: Differential microRNA signature of human mesenchymal stem cells from different sources reveals an "environmental-niche memory" for bone marrow stem cells.
abstract: Human mesenchymal stem cells (MSCs) are multipotent cells offering valuable hopes for the treatment of degenerative diseases. MSCs can be found among differentiated cells in many tissues and organs but, unfortunately, their phenotypic similarity hinders a robust cell characterization and discrimination from diverse tissue harvests. MicroRNAs (miRNAs) are crucial managers of gene expression with intriguing and still poorly known roles in stem cell maintenance and differentiation. To identify miRNAs that can discriminate among MSCs, we performed a whole-genome comparative miRNA expression profiling analysis on adipose (AD), bone marrow (BM) and cord blood (CB) derived MSCs, all three considered among the most promising in the field of regenerative medicine. miRNA expression patterns were very similar, meeting their extensive phenotypic and functional overlaps. An in-depth comparison of the few most differentially expressed miRNAs allowed the identification of a highly restricted molecular signature consisting of 5 BMMSC, 11 ADMSC and 11 CBMSC specific miRNAs. Functional analysis of their validated targets allowed the identification of an "environmental-niche memory" for BMMSC and an "epithelial" commitment for ADMSC, providing new insights into the molecular mechanisms discriminating between these MSCs, a crucial element to identify the most appropriate stem cell source for clinical application.
authors.affiliation: Cell Factory Franco Calori, Center of Transfusion Medicine, Cellular Therapy and Cryobiology, Department of Regenerative Medicine, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Via F. Sforza 35, 20122 Milano, Italy. enrico.ragni@policlinico.mi.it
authors.forename: Enrico
authors.initials: E
authors.lastname: Ragni
authors.forename: Tiziana
authors.initials: T
authors.lastname: Montemurro
authors.forename: Elisa
authors.initials: E
authors.lastname: Montelatici
authors.forename: Cristiana
authors.initials: C
authors.lastname: Lavazza
authors.forename: Mariele
authors.initials: M
authors.lastname: Viganò
authors.forename: Paolo
authors.initials: P
authors.lastname: Rebulla
authors.forename: Rosaria
authors.initials: R
authors.lastname: Giordano
authors.forename: Lorenza
authors.initials: L
authors.lastname: Lazzari
mesh_terms: D050156:Adipogenesis; D000273:Adipose Tissue / Q000737:chemistry / Q000166:cytology; D001853:Bone Marrow / Q000737:chemistry*; D016923:Cell Death; D049109:Cell Proliferation; D048430:Cell Shape; D002470:Cell Survival; D020219:Chondrogenesis; D003470:Culture Media / Q000737:chemistry; D005312:Fetal Blood / Q000737:chemistry / Q000166:cytology; D005434:Flow Cytometry; D020869:Gene Expression Profiling / Q000379:methods; D015894:Genome, Human; D006412:Hematopoietic Stem Cells / Q000737:chemistry / Q000166:cytology*; D006801:Humans; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000737:chemistry / Q000166:cytology*; D035683:MicroRNAs / Q000235:genetics*; D010012:Osteogenesis; D055153:Stem Cell Niche
chemical_list: D003470:Culture Media; D035683:MicroRNAs