title: Acute and subacute toxicity studies on triptolide and triptolide-loaded polymeric micelles following intravenous administration in rodents.
abstract: Except its anti-tumour effects, triptolide (TP) also shows multiple pharmacological side activities, such as immune-suppressive and male anti-fertility. To increase the therapeutic index of TP, a novel polymeric micelle system containing TP (TP-PM) has been developed to treat tumour. Our previous studies have demonstrated the good anti-tumour efficacy of TP-PM. This paper investigated the acute toxicity in mice and subacute toxicity in rats of TP-PM and TP. Results demonstrated that the LD50 for TP-PM and TP administered intravenously were 1.06 mg/kg and 0.83 mg/kg in mice, respectively. In subacute toxicity study, TP-PM and TP were administered intravenously at the dose levels of 0.1 mg/kg and 0.3 mg/kg for 14 d. Compared to the control, there was significant decrease in the serum AST activities, the testis ACP activities, thymus index, testis index, and significant increase in spleen index, and obvious histopathological changes in rats treated with TP, however, the toxicities of TP-PM on liver, kidney, testis and spleen are slighter than TP. Compared to TP, TP-PM significantly increased the ACP activity of the testis and decreased the MDA level in serum. So, the polymeric micelles may be a novel drug delivery carrier of TP for reducing the toxicities of TP.
authors.affiliation: College of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, PR China.
authors.forename: Lingyun
authors.initials: L
authors.lastname: Xu
authors.forename: Yinsheng
authors.initials: Y
authors.lastname: Qiu
authors.forename: Huibi
authors.initials: H
authors.lastname: Xu
authors.forename: Wuliji
authors.initials: W
authors.lastname: Ao
authors.forename: Waikei
authors.initials: W
authors.lastname: Lam
authors.forename: Xiangliang
authors.initials: X
authors.lastname: Yang
mesh_terms: D061605:Administration, Intravenous; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D004224:Diterpenes / Q000008:administration & dosage* / Q000633:toxicity*; D004337:Drug Carriers; D016503:Drug Delivery Systems; D004852:Epoxy Compounds / Q000008:administration & dosage / Q000633:toxicity; D005260:Female; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D007928:Lethal Dose 50; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D051379:Mice; D008823:Micelles; D010616:Phenanthrenes / Q000008:administration & dosage* / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D012377:Rodentia; D013154:Spleen / Q000187:drug effects / Q000473:pathology; D013737:Testis / Q000187:drug effects / Q000473:pathology; D023382:Toxicity Tests, Acute / Q000379:methods; D059551:Toxicity Tests, Subacute / Q000379:methods
chemical_list: D004224:Diterpenes; D004337:Drug Carriers; D004852:Epoxy Compounds; D008823:Micelles; D010616:Phenanthrenes; C001899:triptolide; D001219:Aspartate Aminotransferases