title: Reflex: intramolecular barcoding of long-range PCR products for sequencing multiple pooled DNAs.
abstract: We present an intramolecular reaction, Reflex™, to derive shorter, sequencer-ready, daughter polymerase chain reaction products from a pooled population of barcoded long-range polymerase chain reaction products, whilst still preserving the cognate DNA barcodes. Our Reflex workflow needs only a small number of primer extension steps to rapidly enable uniform sequence coverage of long contiguous sequence targets in large numbers of samples at low cost on desktop next-generation sequencers.
authors.affiliation: Population Genetics Technologies Ltd., Babraham Institute, Babraham, Cambridgeshire CB22 3AT, UK.
authors.forename: James A
authors.initials: JA
authors.lastname: Casbon
authors.forename: Andrew F
authors.initials: AF
authors.lastname: Slatter
authors.forename: Esther
authors.initials: E
authors.lastname: Musgrave-Brown
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Osborne
authors.forename: Conrad P
authors.initials: CP
authors.lastname: Lichtenstein
authors.forename: Sydney
authors.initials: S
authors.lastname: Brenner
mesh_terms: D019389:Cytochrome P-450 CYP2D6 / Q000235:genetics; D017931:DNA Primers / Q000737:chemistry; D006801:Humans; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D017931:DNA Primers; D019389:Cytochrome P-450 CYP2D6