title: Structure of the alanopine-containing O-polysaccharide and serological cross-reactivity of the lipopolysaccharide of Proteus vulgaris HSC 438 classified into a new Proteus serogroup, O76.
abstract: The O-polysaccharide was isolated by mild acid hydrolysis of the lipopolysaccharide of Proteus vulgaris HSC 438, and the following structure was established by chemical methods and one- and two-dimensional (1)H and (13)C NMR spectroscopy: →3)-β-d-Quip4NAlo-(1→3)-α-d-Galp6Ac-(1→6)-α-d-Glcp-(1→3)-α-l-FucpNAc-(1→3)-β-d-GlcpNAc-(1→, where d-Qui4N stands for 4-amino-4,6-dideoxy-d-glucose and Alo for N-((S)-1-carboxyethyl)-l-alanine (alanopine); only about half of the Gal residues are O-acetylated. This structure is unique among the Proteus O-polysaccharides, and therefore it is proposed to classify P. vulgaris HSC 438 into a new Proteus serogroup, O76. A serological cross-reactivity of HSC 438 O-antiserum and lipopolysaccharides of some other Proteus serogroups was observed and accounted for by shared epitopes on the O-polysaccharides or lipopolysaccharide core regions, including that associated with d-Qui4NAlo.
authors.affiliation: Department of General Microbiology, Institute of Microbiology, Biotechnology and Immunology, University of Łódź, ul. Narutowicza 65, 90-131 Łódź, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Siwińska
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 119991 Moscow, Russia.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 119991 Moscow, Russia.
authors.forename: Anna N
authors.initials: AN
authors.lastname: Kondakova
authors.affiliation: Department of General Microbiology, Institute of Microbiology, Biotechnology and Immunology, University of Łódź, ul. Narutowicza 65, 90-131 Łódź, Poland.
authors.forename: Dominika
authors.initials: D
authors.lastname: Drzewiecka
authors.affiliation: Department of General Microbiology, Institute of Microbiology, Biotechnology and Immunology, University of Łódź, ul. Narutowicza 65, 90-131 Łódź, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Zabłotni
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 119991 Moscow, Russia.
authors.forename: Nikolay P
authors.initials: NP
authors.lastname: Arbatsky
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 119991 Moscow, Russia.
authors.forename: Olga A
authors.initials: OA
authors.lastname: Valueva
authors.affiliation: Department of General Microbiology, Institute of Microbiology, Biotechnology and Immunology, University of Łódź, ul. Narutowicza 65, 90-131 Łódź, Poland.
authors.forename: Krystyna
authors.initials: K
authors.lastname: Zych
authors.affiliation: Department of General Microbiology, Institute of Microbiology, Biotechnology and Immunology, University of Łódź, ul. Narutowicza 65, 90-131 Łódź, Poland.
authors.forename: Zygmunt
authors.initials: Z
authors.lastname: Sidorczyk
authors.affiliation: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 119991 Moscow, Russia.
authors.forename: Yuriy A
authors.initials: YA
authors.lastname: Knirel
mesh_terms: D000409:Alanine / Q000031:analogs & derivatives* / Q000032:analysis; D003429:Cross Reactions; D008070:Lipopolysaccharides / Q000276:immunology*; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry* / Q000276:immunology; D011514:Proteus vulgaris / Q000737:chemistry* / Q000145:classification / Q000276:immunology*; D012703:Serotyping
chemical_list: D008070:Lipopolysaccharides; D019081:O Antigens; C026051:alanopine; D000409:Alanine