title: Serum cytokine profile in patients with hepatitis B e antigen-negative chronic active hepatitis B and inactive hepatitis B virus carriers.
abstract: An insufficient cellular immune response seems to be critical for the immunopathogenesis of chronic hepatitis B virus infection. We have previously demonstrated no differences of T-lymphocyte subsets in blood between inactive hepatitis B s antigen (HBsAg) carriers and patients with HBeAg-negative chronic active hepatitis B. This study investigated the peripheral blood cytokine profile in patients with HBeAg-negative chronic active hepatitis B infection (Group A, n = 21) and inactive HBsAg carriers (Group B, n = 13). Serum cytokines [interferon (IFN)-γ, tumor necrosis factor-α, interleukin (IL)-1b, IL-4, IL-12, IL-10, IL-2, IL-5, IL-8] were analyzed by using flow cytometry. Patients with chronic active disease presented with significantly decreased levels of IFN-γ and IL-10 compared to inactive carriers (P = 0.048 and P = 0.008, respectively). In HBeAg-negative chronic active hepatitis B patients, a significant negative correlation of IFN-γ levels with serum hepatitis B viral load was noted (P = 0.021). In conclusion, patients with HBeAg-negative chronic active hepatitis B and HBsAg inactive carriers display a different cytokine profile. Decreased Th1 response observed in patients with chronic active hepatitis B could be implicated in the persistence of virus replication and ongoing progression of liver disease.
authors.affiliation: Dimitra Dimitropoulou, Lydia Leonidou, Stelios F Assimakopoulos, Charalambos A Gogos, Department of Internal Medicine, University Hospital of Patras, 26504 Patras, Greece.
authors.forename: Dimitra
authors.initials: D
authors.lastname: Dimitropoulou
authors.forename: Marina
authors.initials: M
authors.lastname: Karakantza
authors.forename: Georgios L
authors.initials: GL
authors.lastname: Theodorou
authors.forename: Lydia
authors.initials: L
authors.lastname: Leonidou
authors.forename: Stelios F
authors.initials: SF
authors.lastname: Assimakopoulos
authors.forename: Athanasia
authors.initials: A
authors.lastname: Mouzaki
authors.forename: Charalambos A
authors.initials: CA
authors.lastname: Gogos
keywords: Cytokines; Flow cytometry; Hepatitis B; Immunoreactive fibronectin-γ