title: Delivery of proteases in aqueous two-phase systems enables direct purification of stem cell colonies from feeder cell co-cultures for differentiation into functional cardiomyocytes.
abstract: Patterning of bioactive enzymes with subcellular resolution is achieved by dispensing droplets of dextran (DEX) onto polyethylene glycol (PEG)-covered cells though a glass capillary needle connected to a pneumatic pump. This technique is applied to purify colonies of induced pluripotent stem cells (iPSCs) from mouse embryonic fibroblast (MEF) feeder cultures and inefficiently induced iPSC colonies by selectively dissociating the iPSCs with proteases.
authors.affiliation: Department of Biomedical Engineering and Department of Macromolecular Science and Engineering, University of Michigan, Ann Arbor, MI 48104, USA.
authors.forename: John P
authors.initials: JP
authors.lastname: Frampton
authors.forename: Huilin
authors.initials: H
authors.lastname: Shi
authors.forename: Albert
authors.initials: A
authors.lastname: Kao
authors.forename: Jack M
authors.initials: JM
authors.lastname: Parent
authors.forename: Shuichi
authors.initials: S
authors.lastname: Takayama
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002469:Cell Separation / Q000379:methods*; D018920:Coculture Techniques / Q000379:methods*; D003911:Dextrans / Q000737:chemistry; D061252:Feeder Cells / Q000166:cytology*; D057809:HEK293 Cells; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D032383:Myocytes, Cardiac / Q000166:cytology*; D010447:Peptide Hydrolases / Q000378:metabolism*; D011092:Polyethylene Glycols / Q000737:chemistry; D014867:Water / Q000737:chemistry*
chemical_list: D003911:Dextrans; D014867:Water; D011092:Polyethylene Glycols; D010447:Peptide Hydrolases
keywords: aqueous two-phase system; cardiomyocyte; dextran; polyethylene glycol; stem cell