title: Multiplex mass spectrometric imaging with polarity switching for concurrent acquisition of positive and negative ion images.
abstract: We have recently developed a multiplex mass spectrometry imaging (MSI) method which incorporates high mass resolution imaging and MS/MS and MS(3) imaging of several compounds in a single data acquisition utilizing a hybrid linear ion trap-Orbitrap mass spectrometer (Perdian and Lee, Anal. Chem. 82, 9393-9400, 2010). Here we extend this capability to obtain positive and negative ion MS and MS/MS spectra in a single MS imaging experiment through polarity switching within spiral steps of each raster step. This methodology was demonstrated for the analysis of various lipid class compounds in a section of mouse brain. This allows for simultaneous imaging of compounds that are readily ionized in positive mode (e.g., phosphatidylcholines and sphingomyelins) and those that are readily ionized in negative mode (e.g., sulfatides, phosphatidylinositols and phosphatidylserines). MS/MS imaging was also performed for a few compounds in both positive and negative ion mode within the same experimental set-up. Insufficient stabilization time for the Orbitrap high voltage leads to slight deviations in observed masses, but these deviations are systematic and were easily corrected with a two-point calibration to background ions.
authors.affiliation: Ames Laboratory, US Department of Energy, Ames, IA 50011, USA.
authors.forename: Andrew R
authors.initials: AR
authors.lastname: Korte
authors.forename: Young Jin
authors.initials: YJ
authors.lastname: Lee
mesh_terms: D000818:Animals; D000838:Anions / Q000737:chemistry*; D001923:Brain Chemistry; D002138:Calibration; D002412:Cations / Q000737:chemistry*; D006651:Histocytochemistry; D051379:Mice; D057054:Molecular Imaging / Q000379:methods*; D010743:Phospholipids / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D000838:Anions; D002412:Cations; D010743:Phospholipids