title: [Simultaneous determination of three antioxidants BHA, BHT and TBHQ in food by liquid chromatography and gas chromatography].
abstract: OBJECTIVE
  To develop a method for determining 3 kinds of antioxidants in food in same time with HPLC and GC.
  
  
  METHODS
  Firstly, extracted fat with petroleum ether, then extracted antioxidants with 13 ml methnol, centrifuged, repeated the above procedure twice, combined the extracts, next evaporated to 5 ml, constanted volume to 10 ml, then kepted in freezer (- 1h), lastly, the supernatant injected into high performance liquid chromatography (HPLC) and Gas chromatography (GC) which had been optimized condition.
  
  
  RESULTS
  The limits of quantitation for BHA,BHT and TBHQ were 0.002, 0.010 and 0.002g/kg respectively in HPLC, The limits of quantitation for BHA, BHT and TBHQ were 0.003, 0.002 and 0.005 g/kg respectively in GC, The mean recoveries at the two spiked levels were 82.8% - 109.0%. There were no significant difference between HPLC and GC.
  
  
  CONCLUSIONS
  The method can be used to determine the antioxidants in food accurately and sensitively, the comparative results are constant between liquid and gas chromatography.
authors.affiliation: China National Centre for Food Safety Risk Assessment, Beijing 100021, China. landuyue@126.com
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Congrong
authors.initials: C
authors.lastname: Fang
authors.forename: Dajin
authors.initials: D
authors.lastname: Yang
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D002083:Butylated Hydroxyanisole / Q000032:analysis*; D002084:Butylated Hydroxytoluene / Q000032:analysis*; D002849:Chromatography, Gas; D002853:Chromatography, Liquid; D005503:Food Additives / Q000032:analysis*; D006873:Hydroquinones / Q000032:analysis*
chemical_list: D000975:Antioxidants; D005503:Food Additives; D006873:Hydroquinones; D002084:Butylated Hydroxytoluene; D002083:Butylated Hydroxyanisole; C018855:2-tert-butylhydroquinone