title: Vagal nerve stimulation protects cardiac injury by attenuating mitochondrial dysfunction in a murine burn injury model.
abstract: Mitochondria play a central role in the integration and execution of a wide variety of apoptotic signals. In the present study, we examined the deleterious effects of burn injury on heart tissue. We explored the effects of vagal nerve stimulation (VNS) on cardiac injury in a murine burn injury model, with a focus on the protective effect of VNS on mitochondrial dysfunction in heart tissue. Mice were subjected to a 30% total body surface area, full-thickness steam burn followed by right cervical VNS for 10 min. and compared to burn alone. A separate group of mice were treated with the M3-muscarinic acetylcholine receptor (M3-AchR) antagonist 4-DAMP or phosphatidylinositol 3 Kinase (PI3K) inhibitor LY294002 prior to burn and VNS. Heart tissue samples were collected at 6 and 24 hrs after injury to measure changes in apoptotic signalling pathways. Burn injury caused significant cardiac pathological changes, cardiomyocyte apoptosis, mitochondrial swelling and decrease in myocardial ATP content at 6 and 24 hrs after injury. These changes were significantly attenuated by VNS. VNS inhibited release of pro-apoptotic protein cytochrome C and apoptosis-inducing factor from mitochondria to cytosol by increasing the expression of Bcl-2, and the phosphorylation level of Bad (pBad(136)) and Akt (pAkt(308)). These protective changes were blocked by 4-DAMP or LY294002. We demonstrated that VNS protected against burn injury-induced cardiac injury by attenuating mitochondria dysfunction, likely through the M3-AchR and the PI3K/Akt signalling pathways.
authors.affiliation: Division of Trauma, Surgical Critical Care and Burns, Department of Surgery, University of California San Diego Health Sciences, San Diego, CA 92103, USA.
authors.forename: Xiaojiong
authors.initials: X
authors.lastname: Lu
authors.forename: Todd
authors.initials: T
authors.lastname: Costantini
authors.forename: Nicole E
authors.initials: NE
authors.lastname: Lopez
authors.forename: Paul L
authors.initials: PL
authors.lastname: Wolf
authors.forename: Ann-Marie
authors.initials: AM
authors.lastname: Hageny
authors.forename: James
authors.initials: J
authors.lastname: Putnam
authors.forename: Brian
authors.initials: B
authors.lastname: Eliceiri
authors.forename: Raul
authors.initials: R
authors.lastname: Coimbra
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D017209:Apoptosis; D051033:Apoptosis Inducing Factor / Q000378:metabolism; D015153:Blotting, Western; D002056:Burns / Q000473:pathology* / Q000517:prevention & control; D045304:Cytochromes c / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008929:Mitochondria, Heart / Q000378:metabolism / Q000473:pathology*; D008933:Mitochondrial Swelling; D009206:Myocardium / Q000201:enzymology / Q000473:pathology*; D032383:Myocytes, Cardiac / Q000201:enzymology / Q000473:pathology; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D015398:Signal Transduction; D055536:Vagus Nerve Stimulation; D051025:bcl-Associated Death Protein / Q000378:metabolism
chemical_list: D051033:Apoptosis Inducing Factor; D019253:Proto-Oncogene Proteins c-bcl-2; D051025:bcl-Associated Death Protein; D000255:Adenosine Triphosphate; D045304:Cytochromes c; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt