title: Comparison of complete polyprotein sequences of two isolates of salmon alphavirus (SAV) type I and their behaviour in a salmonid cell line.
abstract: Salmon pancreas disease virus is an alphavirus (family Togaviridae) affecting mainly Atlantic salmon (Salmo salar L.). Both polyprotein sequences of the Scottish isolate (SAV4640) were determined and compared with those of Irish isolate SAVF93-125. High amino acid sequence similarity (99.4 %) was found. Six amino acid deletions were found in the E2 gene of SAV4640. SAVF93-125 demonstrated a high viral load in culture despite high Mx expression. Approximately 50 % of cells infected with SAVF93-125 exhibited a cytopathic effect by day 8. SAV4640 successfully entered the cells, inducing 10,500-fold higher Mx expression at day 2 compared to SAVF93-25; however, no replication was observed based on results of the nsP1 qRT-PCR.
authors.affiliation: Marine Scotland Science, 375 Victoria Road, Aberdeen, AB11 9DB, Scotland, UK, Iveta.Matejusova@scotland.gsi.gov.uk.
authors.forename: Iveta
authors.initials: I
authors.lastname: Matejusova
authors.forename: Katherine
authors.initials: K
authors.lastname: Lester
authors.forename: Ziduo
authors.initials: Z
authors.lastname: Li
authors.forename: Jimena
authors.initials: J
authors.lastname: Bravo
authors.forename: Fiona
authors.initials: F
authors.lastname: Bland
authors.forename: Bertrand
authors.initials: B
authors.lastname: Collet
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D005814:Genes, Viral; D008969:Molecular Sequence Data; D012487:Salmonidae; D014035:Togaviridae / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D014764:Viral Proteins