title: Ovicidal activity of Atalantia monophylla (L) Correa against Spodoptera litura Fab. (Lepidoptera: Noctuidae).
abstract: OBJECTIVE
  To evaluate the efficacy of Atalantia monophylla (A. monophylla) leaf in different solvent crude extracts and fractions against eggs of Spodoptera litura (S. litura).
  
  
  METHODS
  Hexane, ethyl acetate and chloroform solvent extracts of A. monophylla leaf and 12 fractions from hexane extract were screened at 5.0%, 2.5%, 1.0% and 0.5% for crude extracts and 1 000, 500, 250 and 125 mg/kg for fractions against the eggs of S. litura for the ovicidal activity. LC50 and LC90 were calculated using probit analysis.
  
  
  RESULTS
  Hexane crude extract showed maximum ovicidal activity of 61.94% at 5.0% concentration with a correlation value of r (2)=0.81, and least LC50 value of 3.06%. Hexane extract was fractionated using silica gel column chromatography and 12 fractions were obtained. Fraction 9 was active which showed maximum ovicidal activity of 75.61% at 1 000 mg/kg with the LC50 value of 318.65 mg/kg and LC90 value of 1 473.31 mg/kg. In linear regression analysis, significant and high correlation (r (2)=0.81%) was seen between concentration and ovicidal activity of hexane crude extracts and its active fraction.
  
  
  CONCLUSIONS
  As per our knowledge, this is the first report for ovicidal activity of A. monophylla against S. litura, A. monophylla could be used for the management of S. litura and other insect pests.
authors.affiliation: Entomology Research Institute, Loyola College, Chennai, 600 034, Tamil Nadu, India.
authors.forename: Kathirvelu
authors.initials: K
authors.lastname: Baskar
authors.forename: Chellaiah
authors.initials: C
authors.lastname: Muthu
authors.forename: Gnanaprakasam Antony
authors.initials: GA
authors.lastname: Raj
authors.forename: Selvadurai
authors.initials: S
authors.lastname: Kingsley
authors.forename: Savarimuthu
authors.initials: S
authors.lastname: Ignacimuthu
mesh_terms: D000818:Animals; D001681:Biological Assay; D006586:Hexanes / Q000737:chemistry; D006801:Humans; D007306:Insecticides / Q000494:pharmacology*; D007915:Lepidoptera / Q000187:drug effects* / Q000254:growth & development; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D027821:Rutaceae / Q000737:chemistry / Q000502:physiology*; D018411:Spodoptera / Q000187:drug effects* / Q000254:growth & development
chemical_list: D006586:Hexanes; D007306:Insecticides; D010936:Plant Extracts
keywords: Atalantia monophylla; Crude extracts; Fractions; Ovicidal activity; Spodoptera litura