title: The relationship between severity of the disease and circulating nucleosomes in psoriasis patients.
abstract: Psoriasis was initially considered to represent a disease of abnormal epidermal keratinocyte proliferation. Proliferation of keratinocytes is restricted by apoptotic cell death to maintain a constant thickness of epidermis. Nucleosomes are mainly released by apoptotic cells. Tumor necrosis factor-α (TNF-α) is an important factor affecting the apoptosis. In the present study, the relationship between TNF-α, nucleosome and the Psoriasis Area and Severity Index (PASI) score was investigated. The patients were divided according to PASI score into three groups (mild, moderately, severe). Serum TNF-α and nucleosome levels were measured using Enzyme-linked immunosorbent assay (ELISA) method. Our findings show a correct relationship between PASI scores and TNF-α and an inverse relationship between nucleosome and PASI score. According to the results obtained from the study, we believe that serum nucleosome levels can be used as a new indicator in follow-up of patients with psoriasis and monitoring of the effectiveness of drugs which used in the treatment of psoriasis.
authors.affiliation: Microbiology Department of Recep Tayyip Erdoğan University, Medical Faculty Hospital, Rize, 53000, Turkey. ar.dilek@hotmail.com
authors.forename: Aziz Ramazan
authors.initials: AR
authors.lastname: Dilek
authors.forename: Nursel
authors.initials: N
authors.lastname: Dilek
authors.forename: Yunus
authors.initials: Y
authors.lastname: Saral
authors.forename: Derya
authors.initials: D
authors.lastname: Yüksel
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D017209:Apoptosis; D015415:Biomarkers / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D015603:Keratinocytes / Q000276:immunology / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009707:Nucleosomes / Q000473:pathology*; D011237:Predictive Value of Tests; D011379:Prognosis; D011565:Psoriasis / Q000097:blood / Q000175:diagnosis / Q000276:immunology / Q000473:pathology*; D012720:Severity of Illness Index; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D015415:Biomarkers; D009707:Nucleosomes; D014409:Tumor Necrosis Factor-alpha