title: Genome engineering in actinomycetes using site-specific recombinases.
abstract: The rational modification of the actinomycetes genomes has a variety of applications in research, medicine, and biotechnology. The use of site-specific recombinases allows generation of multiple mutations, large DNA deletions, integrations, and inversions and may lead to significant progress in all of these fields. Despite their huge potential, site-specific recombinase-based technologies have primarily been used for simple marker removal from a chromosome. In this review, we summarise the site-specific recombination approaches for genome engineering in various actinomycetes.
authors.affiliation: Helmholtz-Institute for Pharmaceutical Research Saarland, Campus, Building C2.3, Saarbrücken 66123, Germany.
authors.forename: Maksym
authors.initials: M
authors.lastname: Myronovskyi
authors.forename: Andriy
authors.initials: A
authors.lastname: Luzhetskyy
mesh_terms: D039903:Actinobacteria / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D005827:Genetics, Microbial / Q000379:methods*; D060847:Metabolic Engineering / Q000379:methods*; D009154:Mutation; D045522:Recombinases / Q000378:metabolism*; D011995:Recombination, Genetic
chemical_list: D004269:DNA, Bacterial; D045522:Recombinases