title: Measles virus-derived peptide/food antigen adducts facilitate the establishment of antigen specific oral tolerance.
abstract: Allergy is a skewed T helper (Th)2 polarization response in the body; its treatment is not satisfactory currently. Oral tolerance dysfunction plays a critical role in the pathogenesis of allergy. The present study aims to restore the breached intestinal tolerance with an artificial adduct of a measles virus C protein-derived small peptide (MVCP) and a model antigen, ovalbumin (MOA), and to observe the effect of MOA on inhibition of intestinal allergy in a mouse model. The MOA was formed by the MVCP and ovalbumin. The effect of MOA on regulation of the properties of dendritic cells (DC) and CD4(+) T cells was observed with a cell culture model and a mouse model of the gut Th2 pattern inflammation. After treatment with MOA, mouse intestinal DCs showed high levels of aldehyde dehydrogenase (ALDH) activity and expressed transforming growth factor (TGF)-beta; the frequency of Treg in the intestine was also significantly increased. The treatment with MOA efficiently suppressed the antigen-specific Th2 pattern inflammation in the intestine. Administration with the MOA can induce the development of antigen-specific oral tolerance and inhibit the antigen-specific allergic reaction in the intestine. The adduct of MOA has the therapeutic potential for the allergen related immune inflammation.
authors.affiliation: Department of Gastroenterology, The 10th Hospital of Shanghai, Tongji University, Shanghai, China.
authors.forename: C
authors.initials: C
authors.lastname: He
authors.forename: C-H
authors.initials: CH
authors.lastname: Song
authors.forename: L
authors.initials: L
authors.lastname: Cheng
authors.forename: T
authors.initials: T
authors.lastname: Chen
authors.forename: C
authors.initials: C
authors.lastname: Liu
authors.forename: Z
authors.initials: Z
authors.lastname: Liu
authors.forename: P-C
authors.initials: PC
authors.lastname: Yang
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000276:immunology; D000485:Allergens / Q000276:immunology*; D000818:Animals; D000941:Antigens / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology; D005512:Food Hypersensitivity / Q000276:immunology* / Q000821:virology*; D007108:Immune Tolerance; D007249:Inflammation / Q000276:immunology; D007422:Intestines / Q000276:immunology; D008459:Measles virus / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000276:immunology; D010455:Peptides / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D016212:Transforming Growth Factor beta / Q000276:immunology; D014764:Viral Proteins / Q000276:immunology*
chemical_list: D000485:Allergens; D000941:Antigens; D010455:Peptides; D016212:Transforming Growth Factor beta; D014764:Viral Proteins; D010047:Ovalbumin; D000444:Aldehyde Dehydrogenase