title: [Identification of calcium/calmodulin-binding receptor-like kinase GsCBRLK-interactive proteins using yeast two-hybrid system].
abstract: GsCBRLK (calcium/calmodulin-binding receptor-like kinase from Glycine soja) links ABA (abscisic acid)- and salt-induced calcium/calmodulin signal in plant cells. In order to study the molecular mechanisms of GsCBLRK, the salt-treated Glycine soja cDNA library was screened with pBT3-STE-CBRLK as bait plasmid using yeast two hybrid system. Two positive clones (SNARE and 14-3-3 protein) were identified by constructing cDNA library of wild soybean under salt treatment, membrane system yeast two hybrid screening, multiple screen, rotary validation, bioinformatic analysis and interaction identification in yeast.
authors.affiliation: College of Life Sciences, Northeast Agriculture University, Harbin, China. yangshanshanhp@sina.com
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Yang
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Sun
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.forename: Hu
authors.initials: H
authors.lastname: Cai
authors.forename: Wei
authors.initials: W
authors.lastname: Ji
authors.forename: Xi
authors.initials: X
authors.lastname: Bai
authors.forename: Yan-Ming
authors.initials: YM
authors.lastname: Zhu
mesh_terms: D002352:Carrier Proteins / Q000378:metabolism*; D019295:Computational Biology / Q000379:methods; D015723:Gene Library; D010940:Plant Proteins / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D013025:Glycine max / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D002352:Carrier Proteins; D010940:Plant Proteins; D017346:Protein Serine-Threonine Kinases