title: Determination of macrocyclic lactones in food and feed.
abstract: A confirmatory high-performance liquid chromatography method with fluorescence detection (HPLC-FLD) was developed and validated for the simultaneous determination of the following anti-parasitic veterinary drugs in foodstuffs (liver, muscle and milk) and feed: abamectin, doramectin, emamectin, eprinomectin, ivermectin and moxidectin. Samples were extracted, purified and analysed by HPLC-FLD after prior derivatisation of analytes with N-methylimidazole, trifluoracetic anhydride and acetic acid to produce stable fluorescent derivatives. Recoveries were in the range of 73-98% (liver), 82-93% (muscle), 77-84% (milk), and 93-110% (feed). In all matrices the coefficients of variation for repeatability and intra-laboratory reproducibility were satisfactory. For emamectin in liver the worst method performance was observed. As this multi-analyte method fully met the required criteria and applicability both to food and feed, it has been successfully applied in National Control Plans in food and feed.
authors.affiliation: Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Perugia, Italy. r.galarini@izsum.it
authors.forename: Roberta
authors.initials: R
authors.lastname: Galarini
authors.forename: Giorgio
authors.initials: G
authors.lastname: Saluti
authors.forename: Simone
authors.initials: S
authors.lastname: Moretti
authors.forename: Danilo
authors.initials: D
authors.lastname: Giusepponi
authors.forename: Guglielmo
authors.initials: G
authors.lastname: Dusi
mesh_terms: D002851:Chromatography, High Pressure Liquid; D007783:Lactones / Q000032:analysis*; D047028:Macrocyclic Compounds / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence
chemical_list: D007783:Lactones; D047028:Macrocyclic Compounds