title: The stimulation of dendritic cells by amyloid beta 1-42 reduces BDNF production in Alzheimer's disease patients.
abstract: Dendritic cells (DCs), the main actors of immune responses and inflammation, may play a role in Alzheimer's disease (AD). Recent studies demonstrate that monocyte-derived DCs (MDDCs), generated in vitro in the presence of amyloid β1-42 peptide (Aβ1-42), show a functional alteration and an increased production of inflammatory molecules. Accordingly, MDDCs from AD patients show a more pronounced pro-inflammatory profile than DCs obtained from control subjects. In this study, we aimed at further investigating DC role in AD. Thus, we analyzed the in vitro effect of Aβ1-42 treatment on already differentiated DCs from AD patients, as compared to control subjects. We found that Aβ1-42 significantly decreases the expression of brain-derived neurotrophic factor (BDNF) in DCs derived from AD patients but not from control subjects. Thus, possibly due to their Aβ-induced reduction of neurotrophic support to neurons, DCs from AD patients might contribute to brain damage by playing a part in Aβ-dependent neuronal toxicity.
authors.affiliation: Department of Clinical and Behavioral Neurology, IRCCS Santa Lucia Foundation, Via Ardeatina 306, 00179 Rome, Italy. a.ciaramella@hsantalucia.it
authors.forename: Antonio
authors.initials: A
authors.lastname: Ciaramella
authors.forename: Francesca
authors.initials: F
authors.lastname: Salani
authors.forename: Federica
authors.initials: F
authors.lastname: Bizzoni
authors.forename: Maria Donata
authors.initials: MD
authors.lastname: Orfei
authors.forename: Roberto
authors.initials: R
authors.lastname: Langella
authors.forename: Francesco
authors.initials: F
authors.lastname: Angelucci
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Spalletta
authors.forename: Anna Rita
authors.initials: AR
authors.lastname: Taddei
authors.forename: Carlo
authors.initials: C
authors.lastname: Caltagirone
authors.forename: Paola
authors.initials: P
authors.lastname: Bossù
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000378:metabolism*; D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D000704:Analysis of Variance; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis*; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000187:drug effects* / Q000378:metabolism; D039721:Diagnostic and Statistical Manual of Mental Disorders; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D009483:Neuropsychological Tests; D010446:Peptide Fragments / Q000494:pharmacology*; D010587:Phagocytosis / Q000502:physiology; D013268:Stimulation, Chemical; D013601:T-Lymphocytes / Q000502:physiology
chemical_list: D016229:Amyloid beta-Peptides; D019208:Brain-Derived Neurotrophic Factor; D016207:Cytokines; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42)