title: Antibacterial activity of the alkaloid-enriched extract from Prosopis juliflora pods and its influence on in vitro ruminal digestion.
abstract: The purpose of this study was to assess the in vitro antimicrobial activity of alkaloid-enriched extracts from Prosopis juliflora (Fabaceae) pods in order to evaluate them as feed additives for ruminants. As only the basic chloroformic extract (BCE), whose main constituents were juliprosopine (juliflorine), prosoflorine and juliprosine, showed Gram-positive antibacterial activity against Micrococcus luteus (MIC = 25 μg/mL), Staphylococcus aureus (MIC = 50 μg/mL) and Streptococcus mutans (MIC = 50 μg/mL), its influence on ruminal digestion was evaluated using a semi-automated in vitro gas production technique, with monensin as the positive control. Results showed that BCE has decreased gas production as efficiently as monensin after 36 h of fermentation, revealing its positive influence on gas production during ruminal digestion. Since P. juliflora is a very affordable plant, this study points out this alkaloid enriched extract from the pods of Prosopis juliflora as a potential feed additive to decrease gas production during ruminal digestion.
authors.affiliation: Department of Instrumental and Basic Studies, State University of Southeast of Bahia, BR 415, Km 03, s/n, 45.700-000 Itapetinga, Bahia, Brazil. ronbatis@ufba.br.
authors.forename: Edilene T
authors.initials: ET
authors.lastname: Dos Santos
authors.forename: Mara Lúcia A
authors.initials: ML
authors.lastname: Pereira
authors.forename: Camilla Flávia P G
authors.initials: CF
authors.lastname: da Silva
authors.forename: Lourdes C
authors.initials: LC
authors.lastname: Souza-Neta
authors.forename: Regina
authors.initials: R
authors.lastname: Geris
authors.forename: Dirceu
authors.initials: D
authors.lastname: Martins
authors.forename: Antônio Euzébio G
authors.initials: AE
authors.lastname: Santana
authors.forename: Luiz Cláudio A
authors.initials: LC
authors.lastname: Barbosa
authors.forename: Herymá Giovane O
authors.initials: HG
authors.lastname: Silva
authors.forename: Giovana C
authors.initials: GC
authors.lastname: Freitas
authors.forename: Mauro P
authors.initials: MP
authors.lastname: Figueiredo
authors.forename: Fernando F
authors.initials: FF
authors.lastname: de Oliveira
authors.forename: Ronan
authors.initials: R
authors.lastname: Batista
mesh_terms: D000470:Alkaloids / Q000008:administration & dosage; D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry*; D002417:Cattle; D004063:Digestion; D005285:Fermentation; D005503:Food Additives / Q000008:administration & dosage / Q000737:chemistry; D066298:In Vitro Techniques; D007212:Indolizines / Q000008:administration & dosage; D008697:Methane / Q000096:biosynthesis; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry; D029862:Prosopis / Q000737:chemistry*; D012417:Rumen / Q000187:drug effects* / Q000382:microbiology / Q000502:physiology*
chemical_list: D000470:Alkaloids; D000900:Anti-Bacterial Agents; D005503:Food Additives; D007212:Indolizines; D010936:Plant Extracts; C508951:juliprosine; C048175:juliflorine; D008697:Methane