title: [Screening of hepatotoxicity fraction of Genkwa Flos and study on UPLC fingerprint of hepatotoxicity fraction].
abstract: OBJECTIVE
  To look for the active fraction of ethanol extract of Genkwa Flos (EGF) induced hepatotoxicity and develop an UPLC fingerprint of the active fraction.
  
  
  METHODS
  Target fraction of EGF induced hepatotoxicity was guided by the serum biochemical and histopathology methods. The UPLC method was applied to establish the chromatographic fingerprint. The separation was achieved on a BEH C18 column (2.1 mm x 50 mm, 1.7 microm) with a mobile phase consisting of acetonitrile and water containing 0.05% phosphate acid running gradient elution. The detection was carried out at 210 nm and the analysis was finished within 10 min.
  
  
  RESULTS
  The chloroform phase of EGF could be responsible for the hepatotoxicity of this herb. The common mode of the UPLC fingerprint was set up under the established condition. There were 17 common peaks in fourteen batches of herbs, eight of which were identified, and the similar degrees of the fourteen batches to the common mode were between 0.890-0.999.
  
  
  CONCLUSIONS
  It is easy to locate the chloroform extraction of EGF with hepatotoxicity. And the UPLC fingerprint was developed for the above fraction, which could provide valuable references for safe and effective clinical use of EGF.
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China. yuerduoduo0814@yahoo.cn
authors.forename: Yang
authors.initials: Y
authors.lastname: Yuan
authors.forename: Lu-Lu
authors.initials: LL
authors.lastname: Geng
authors.forename: He-Fei
authors.initials: HF
authors.lastname: Zhuang
authors.forename: Xia
authors.initials: X
authors.lastname: Meng
authors.forename: Ying
authors.initials: Y
authors.lastname: Peng
authors.forename: Kai-Shun
authors.initials: KS
authors.lastname: Bi
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Chen
mesh_terms: D000818:Animals; D019659:Asteraceae / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000633:toxicity*; D035264:Flowers / Q000737:chemistry*; D006801:Humans; D008099:Liver / Q000187:drug effects*; D008297:Male; D051381:Rats; D017208:Rats, Wistar
chemical_list: D004365:Drugs, Chinese Herbal