title: Marinobacter persicus sp. nov., a moderately halophilic bacterium from a saline lake in Iran.
abstract: A Gram-negative, non-endospore-forming, rod shaped, strictly aerobic, moderately halophilic bacterium, designated strain M9B(T), was isolated from the hypersaline lake Aran-Bidgol in Iran. Cells of strain M9B(T) were found to be motile and produce colonies with an orange-yellow pigment. Growth was determined to occur between 5 and 20 % (w/v) NaCl and the isolate grew optimally at 7.5-10 % (v/w) NaCl. The optimum pH and temperature for growth of the strain were determined to be pH 7.0 and 35 °C, respectively, while it was able to grow over pH and temperature ranges of 6-8 and 25-45 °C, respectively. Phylogenetic analysis based on the comparison of 16S rRNA gene sequences revealed that strain M9B(T) is a member of the genus Marinobacter. The closest relative to this strain was found to be Marinobacter hydrocarbonoclasticus MBIC 1303(T) with a similarity level of 97.7 %. DNA-DNA hybridization between the novel isolate and this phylogenetically related species was 13 ± 2 %. The major cellular fatty acids of the isolate were identified as C16:0, C19:1 ω6c, C18:1 ω9c and C16:1 ω9c. The polar lipid pattern of strain M9B(T) was determined to consist of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylserine and three phospholipids. Ubiquinone 9 (Q-9) was the only lipoquinone detected. The G+C content of the genomic DNA of this strain was determined to be 58.6 mol%. Phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data suggest that this strain represents a novel species of the genus Marinobacter, for which the name Marinobacter persicus sp. nov. is proposed. The type strain of Marinobacter persicus is strain M9B(T) (=IBRC-M 10445(T) = CCM 7970(T) = CECT 7991(T) = KCTC 23561(T)).
authors.affiliation: Extremophiles Laboratory, Department of Microbiology, Faculty of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, P. O. Box 14155-6455, Tehran, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Bagheri
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Amoozegar
authors.forename: Maryam
authors.initials: M
authors.lastname: Didari
authors.forename: Ali
authors.initials: A
authors.lastname: Makhdoumi-Kakhki
authors.forename: Peter
authors.initials: P
authors.lastname: Schumann
authors.forename: Cathrin
authors.initials: C
authors.lastname: Spröer
authors.forename: Cristina
authors.initials: C
authors.lastname: Sánchez-Porro
authors.forename: Antonio
authors.initials: A
authors.lastname: Ventosa
mesh_terms: D001482:Base Composition; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D007492:Iran; D060106:Lakes; D008055:Lipids / Q000032:analysis; D053520:Marinobacter / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013696:Temperature; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D008055:Lipids; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S