title: Comparison of the cytokine immune response to pathogenic Yersinia enterocolitica bioserotype 1B/O:8 and 2/O:9 in susceptible BALB/C and resistant C57BL/6 mice.
abstract: We investigated the lethality of pathogenic Yersinia enterocolitica bioserotypes 1B/O:8 and 2/O:9 in susceptible BALB/C and resistant C57BL/6 mice; the cytokine alterations and histopathological changes were observed comparing the two strains in BALB/C mice. The data showed the 50% lethal dose (LD50) for the pathogenic Y. enterocolitica bioserotype 1B/O:8 was 10³ cfu in both BALB/C and C57BL/6 mice; while the LD50 for the 2/O:9 was 10⁸ cfu in BALB/C mice and 10⁹ cfu in C57BL/6 mice, a large difference. After infection with the two strains in BALB/C mice, GM-CSF (granulocyte-macrophage colony stimulating factor), IFN-γ (interferon-γ), IL-1β (interleukin-1β), IL-2, IL-4, IL-5, IL-6, IL-10, IL-12, and TNF-α (tumor necrosis factor-α) appeared as a cytokine storm in a short period, reached peak values, and then quickly decreased. This appeared important for the immune response and cytokine immunopathogenesis in pathogenic Y. enterocolitica infections. In the initial infection stage, GM-CSF, IL-6, and TNF-α of 2/O:9 were higher than 1B/O:8; and subsequently the status was reversed. However, levels of IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-10, IL-12 following infection with 1B/O:8 were always higher than with 2/O:9. The histopathological changes in the liver and spleen in BALB/C mice infected with the two strains were similar at different times and doses. These observations show the different immunological effects and changes for pathogenic Y. enterocolitica 1B/O:8 and 2/O:9 infections using the mouse model.
authors.affiliation: National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, State Key Laboratory for Infectious Disease Prevention and Control, 102206 Beijing, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Wenpeng
authors.initials: W
authors.lastname: Gu
authors.forename: Haiyan
authors.initials: H
authors.lastname: Qiu
authors.forename: Shengli
authors.initials: S
authors.lastname: Xia
authors.forename: Han
authors.initials: H
authors.lastname: Zheng
authors.forename: Yuchun
authors.initials: Y
authors.lastname: Xiao
authors.forename: Junrong
authors.initials: J
authors.lastname: Liang
authors.forename: Huaiqi
authors.initials: H
authors.lastname: Jing
mesh_terms: D000818:Animals; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D008099:Liver / Q000276:immunology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D012703:Serotyping; D013045:Species Specificity; D013154:Spleen / Q000276:immunology / Q000473:pathology; D014774:Virulence / Q000276:immunology; D015009:Yersinia Infections / Q000276:immunology* / Q000382:microbiology / Q000473:pathology; D015008:Yersinia enterocolitica / Q000145:classification / Q000276:immunology* / Q000472:pathogenicity*
chemical_list: D016207:Cytokines