title: [Cloning and sequence analysis of cDNA encoding aquaporin (AQP) gene from Anopheles sinensis].
abstract: OBJECTIVE
  To clone and analyze the full-length sequence of aquaporin gene of Anopheles sinensis (AsAQP), so as to provide an insight into its biology functions.
  
  
  METHODS
  The degenerate primers were used to amplify conserved region of AQP from An. sinensis cDNA. After then, the full-length cDNA of AsAQP was obtained by rapid amplification of cDNA ends (RACE). Concurrently, the bioinformatics methods were applied to analyze the obtained sequence.
  
  
  RESULTS
  The obtained full-length cDNA of AsAQP consisted of 762 bp and 253 deduced amino acids with a predicted molecular mass of 63.2 kD. Bioinformatics analysis demonstrated that AsAQP had a typical structure with six membrane-spanning domains and an internal symmetry showing two highly conserved Asn-Pro-Ala (NPA) motif and possessing the consensus sequence of major intrinsic protein (MIP) superfamily. The AsAQP shared the identities of 76% and 78% with those of Culex quinquefasciatus and Aedes aegypti AQPs, respectively. Phylogenetic analysis indicated that AsAQP was clustered with Aedes and Culex AQPs.
  
  
  CONCLUSIONS
  The full-length AsAQP is cloned by degenerate primers and RACE from An. sinensis. The AsAQP gene is a member of MIP protein family, and has the typical function region. The study lays the foundation for further research on the function of AsAQP.
authors.affiliation: Jiangsu Institute of Parasitic Diseases, Key Laboratory on Technology for Parasitic Disease Prevention and Control, Ministry of Health, Wuxi 214064, China.
authors.forename: Jian-Xia
authors.initials: JX
authors.lastname: Tang
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.forename: Liang
authors.initials: L
authors.lastname: Bai
authors.forename: Ju-Lin
authors.initials: JL
authors.lastname: Li
authors.forename: Kun
authors.initials: K
authors.lastname: Liu
authors.forename: Hua-Yun
authors.initials: HY
authors.lastname: Zhou
authors.forename: Jun
authors.initials: J
authors.lastname: Cao
authors.forename: Qi
authors.initials: Q
authors.lastname: Gao
mesh_terms: D000818:Animals; D000852:Anopheles / Q000235:genetics*; D020346:Aquaporins / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D020346:Aquaporins; D018076:DNA, Complementary