title: Preventing the mixed detergent micelle effect in two-dimensional electrophoresis on Tris-Tricine gels.
abstract: Application of Tris-N-[Tris(hydroxymethyl)methyl]glycine gels for 2DE is hampered by formation of mixed CHAPS-SDS micelles resulting in typical swirling pattern in the low mass range, which makes reliable quantitative and qualitative gel evaluation impossible. Modification of 2DE strip equilibration procedure prevented the direct interaction between both detergents during equilibration process, thus substantially improving gel separation.
authors.affiliation: Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
authors.forename: Hana
authors.initials: H
authors.lastname: Konečná
authors.forename: Dana
authors.initials: D
authors.lastname: Fridrichová
authors.forename: Gabriela
authors.initials: G
authors.lastname: Lochmanová
authors.forename: Jan
authors.initials: J
authors.lastname: Bárta
authors.forename: Zbyněk
authors.initials: Z
authors.lastname: Zdráhal
mesh_terms: D003902:Detergents / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005998:Glycine / Q000031:analogs & derivatives* / Q000737:chemistry; D008823:Micelles; D010940:Plant Proteins / Q000032:analysis; D020543:Proteome / Q000032:analysis; D040901:Proteomics / Q000379:methods; D011198:Solanum tuberosum / Q000737:chemistry
chemical_list: D003902:Detergents; D008823:Micelles; D010940:Plant Proteins; D020543:Proteome; D005998:Glycine; C100184:tricine
keywords: 2DE; Mixed detergent micelles; Tris-Tricine gel