title: MicroRNA profiling during rat ventricular maturation: A role for miR-29a in regulating cardiomyocyte cell cycle re-entry.
abstract: Recent studies demonstrated that the mammalian heart possesses some capacity to proliferate. We observed cardiomyocyte proliferation within 4 weeks of age (P4W) in rats. We found 95 microRNAs that are differentially expressed in P4W cardiomyocytes. MicroRNA-29a was among the most highly up-regulated microRNAs in P4W cardiomyocytes. Overexpression of microRNA-29a suppressed the proliferation of H9c2 cell line. MicroRNA-29a inhibition induced cardiomyocytes to proliferate, accelerated the G1/S and G2/M transition, and up-regulated the cell cycle gene expression. Cyclin D2 (CCND2) was identified as a direct target of microRNA-29a. These findings indicate that microRNA-29a is involved in cardiomyocyte proliferation during postnatal development.
authors.affiliation: State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Disease, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100037, China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Cao
authors.forename: Jue
authors.initials: J
authors.lastname: Wang
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Wang
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.forename: Xin
authors.initials: X
authors.lastname: Yuan
authors.forename: Weicong
authors.initials: W
authors.lastname: Huang
authors.forename: Jian
authors.initials: J
authors.lastname: Meng
authors.forename: Haiyong
authors.initials: H
authors.lastname: Gu
authors.forename: Yu
authors.initials: Y
authors.lastname: Nie
authors.forename: Bingyang
authors.initials: B
authors.lastname: Ji
authors.forename: Shengshou
authors.initials: S
authors.lastname: Hu
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zheng
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D002453:Cell Cycle / Q000235:genetics*; D002455:Cell Division / Q000235:genetics; D049109:Cell Proliferation; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006352:Heart Ventricles / Q000254:growth & development* / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics* / Q000502:physiology; D046228:Microarray Analysis; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C526366:MIRN29 microRNA, rat; D035683:MicroRNAs