title: Trehangelins A, B and C, novel photo-oxidative hemolysis inhibitors produced by an endophytic actinomycete, Polymorphospora rubra K07-0510.
abstract: Three new natural products, designated trehangelins A, B and C, were isolated by solvent extraction, silica gel and octadecylsilyl silica gel column chromatographies and subsequent preparative HPLC from the cultured broth of an endophytic actinomycete strain, Polymorphospora rubra K07-0510. The trehangelins consisted of a trehalose moiety and two angelic acid moieties. Trehangelins A (IC50 value, 0.1 mg ml(-1)) and C (IC50 value, 0.4 mg ml(-1)), with symmetric structures, showed potent inhibitory activity against hemolysis of red blood cells induced by light-activated pheophorbide a. However, trehangelin B, with an asymmetric structure, displayed only a slight inhibition (IC50 value, 1.0 mg ml(-1)).
authors.affiliation: Research Organization for Infection Control Sciences, Kitasato University, Tokyo 108-8641, Japan. takuji@lisci.kitasato-u.ac.jp
authors.forename: Takuji
authors.initials: T
authors.lastname: Nakashima
authors.forename: Ryuki
authors.initials: R
authors.lastname: Okuyama
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Kamiya
authors.forename: Atsuko
authors.initials: A
authors.lastname: Matsumoto
authors.forename: Masato
authors.initials: M
authors.lastname: Iwatsuki
authors.forename: Yuki
authors.initials: Y
authors.lastname: Inahashi
authors.forename: Kenzaburo
authors.initials: K
authors.lastname: Yamaji
authors.forename: Yōko
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ōmura
mesh_terms: D039903:Actinobacteria / Q000737:chemistry*; D001688:Biological Products / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D055598:Chemical Phenomena; D002734:Chlorophyll / Q000009:adverse effects / Q000031:analogs & derivatives; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D060026:Endophytes / Q000737:chemistry; D004912:Erythrocytes / Q000187:drug effects; D057809:HEK293 Cells; D019073:HT29 Cells; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D020128:Inhibitory Concentration 50; D008968:Molecular Conformation; D029595:Orchidaceae / Q000382:microbiology; D018384:Oxidative Stress / Q000187:drug effects*; D018517:Plant Roots / Q000382:microbiology; D011837:Radiation-Protective Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D011838:Radiation-Sensitizing Agents / Q000009:adverse effects; D014199:Trehalose / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D001688:Biological Products; D011837:Radiation-Protective Agents; D011838:Radiation-Sensitizing Agents; C583207:trehangelin A; C583208:trehangelin B; C583209:trehangelin C; D002734:Chlorophyll; D014199:Trehalose; C032623:pheophorbide a