title: Prevalence of neutralizing factors against adeno-associated virus types 2 in age-related macular degeneration and polypoidal choroidal vasculopathy.
abstract: Adeno-associated virus type 2 (AAV2) mediated gene therapy providing a potential treatment in the eye. However, immune responses can limit virally mediated gene transfer and therapy. To assess preexisting AAV2 neutralizing factors (NF) titers in peripheral blood and the vitreous in patients with age-related macular degeneration (AMD) and polypoidal choroidal vasculopathy (PCV). 130 subjects were enrolled: 50 with neovascular AMD, 30 with PCV, and 50 controls. The serum and the vitreous were obtained for AAV2 NF assay. We found AAV2 NF are present in all of AMD, PCV patients and controls we tested. There were no significant differences in prevalence of NAb in serum between AMD, PCV and controls (P=0.999). There was no correlation between NF in serum and in vitreous (P>0.05), and NF in vitreous was significantly less than in serum. Our results for the first time showed in Chinese population, NF against AAV2 was present in serum of all the patients with AMD or PCV and controls, and there were no significant differences among these groups. Therefore, it demonstrated there were no correlations between AAV2 NF titer and these diseases. We found NF in vitreous was considerably less than in serum in all groups. We also found no direct correlation between NF in vitreous and in serum suggesting serum antibody levels may not be used to predict their counterparts in the vitreous. Our results will provide crucial information for future clinical studies in the development of new therapies based on AAV2 mediated gene delivery in the eye.
authors.affiliation: Department of Ophthalmology, People's Hospital, Peking University, 1 No.11 of XiZhiMen South Avenue, XiCheng District, Beijing, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Cheng
authors.forename: Lvzhen
authors.initials: L
authors.lastname: Huang
authors.forename: Xiaoxin
authors.initials: X
authors.lastname: Li
authors.forename: Huijun
authors.initials: H
authors.lastname: Qi
authors.forename: Peng
authors.initials: P
authors.lastname: Zhou
authors.forename: Wenzhen
authors.initials: W
authors.lastname: Yu
authors.forename: Yide A
authors.initials: YA
authors.lastname: Jiang
authors.forename: Samuel
authors.initials: S
authors.lastname: Wadsworth
authors.forename: Abraham
authors.initials: A
authors.lastname: Scaria
mesh_terms: D000368:Aged; D016022:Case-Control Studies; D020256:Choroidal Neovascularization / Q000821:virology*; D000229:Dependovirus / Q000235:genetics / Q000276:immunology*; D005260:Female; D015316:Genetic Therapy / Q000379:methods; D006801:Humans; D008268:Macular Degeneration / Q000821:virology*; D008297:Male; D008875:Middle Aged; D009500:Neutralization Tests / Q000379:methods; D016491:Peripheral Vascular Diseases / Q000821:virology*; D044967:Serum / Q000276:immunology; D014822:Vitreous Body / Q000821:virology