title: Investigation of the expression of mediators of neovascularization from mononuclear leukocytes in thromboangiitis obliterans.
abstract: The aim of this study was to investigate the expression of the cytokines, chemokines and effective molecules of peripheral blood mononuclear cells (PBMCs) that play a role in neovascularization in thromboangiitis obliterans (TAO). Lymphocytes from TAO patients (n = 20) and control subjects (healthy smokers [n = 16] and non-smokers [n = 17]) were evaluated using realtime polymerase chain reaction in order to examine the mRNA expression of CXCL1 and interleukin 8 (IL-8; inducers of collateral development by recruitment of circulating progenitor cells [CPCs]), endothelial cell growth factor A (VEGF-A) and inducible nitric oxide synthase (iNOS; inducers of angiogenesis) and interferon gamma (IFN-γ) and vascular endothelial growth factor receptor 1 (VEGFR-1; inhibitors of angiogenesis). CXCL1 expression was significantly higher in the TAO patients than control subjects. The expressions of IL-8, VEGFR-1 and IFN-γ were significantly higher in the TAO patients and smokers than in non-smokers. However, no differences in iNOS and VEGF-A expression were noted. In conclusion, PBMCs from TAO patients expressed cytokines that potentially recruit CPCs and promote arteriogenesis. However, TAO patients typically have low CPC levels, perhaps due to high oxidative stress. Further studies are recommended in order to investigate the efficacy of antioxidant therapy on the outcome of TAO before administration of angiogenic factors.
authors.forename: Bahare
authors.initials: B
authors.lastname: Fazeli
authors.forename: Houshang
authors.initials: H
authors.lastname: Rafatpanah
authors.forename: Hassan
authors.initials: H
authors.lastname: Ravari
authors.forename: Reza Farid
authors.initials: RF
authors.lastname: Hosseini
authors.forename: S A Rahim
authors.initials: SA
authors.lastname: Rezaee
mesh_terms: D000328:Adult; D054360:Chemokine CXCL1 / Q000235:genetics*; D003097:Collateral Circulation / Q000502:physiology*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics*; D007963:Leukocytes, Mononuclear / Q000502:physiology; D008297:Male; D018919:Neovascularization, Physiologic / Q000502:physiology*; D018384:Oxidative Stress / Q000502:physiology; D012907:Smoking / Q000097:blood*; D013234:Stem Cells / Q000502:physiology; D013919:Thromboangiitis Obliterans / Q000097:blood* / Q000209:etiology / Q000523:psychology; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000235:genetics*
chemical_list: D054360:Chemokine CXCL1; D016209:Interleukin-8; C501162:FLT1 protein, human; D040281:Vascular Endothelial Growth Factor Receptor-1