title: Isolation and characterization of endophytic fungi from Camptotheca acuminata.
abstract: In this study, a total of 161 endophytic fungal isolates from Camptotheca acuminata were obtained and classified to 16 taxa according to morphological and molecular analysis. These taxa were composed of 2 frequent genera (Botryosphaeria and Fusarium) and 14 infrequent groups such as Xylaria, Diaporthe, Rhizopus, Epicoccum, and Preussia, demonstrating that fungal endophytes in C. acuminata were highly abundant and diverse. Antimicrobial activity screening using filter-paper diffusion method showed that 47.6 % of the tested isolates had antimicrobial activity against at least one of the test microorganisms. Screening of fungal endophyte-derived camptothecin analogues by TLC and LC-MS/MS³ demonstrated that a strain Botryosphaeria dothidea, X-4 could produce 9-methoxycamptothecin (9-MCPT) when cultured in Sabouraud's dextrose broth for 12 days under shake flask and bench-scale fermention conditions. This work showed that the fungal endophytes from C. acuminata could be an alternative source for the production of 9-MCPT and other natural antimicrobial compounds.
authors.affiliation: School of Life Sciences, Yunnan University, Kunming, 650091, China.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Ding
authors.forename: Kaihui
authors.initials: K
authors.lastname: Liu
authors.forename: Baiwan
authors.initials: B
authors.lastname: Deng
authors.forename: Wenqiang
authors.initials: W
authors.lastname: Chen
authors.forename: Wenjun
authors.initials: W
authors.lastname: Li
authors.forename: Feihu
authors.initials: F
authors.lastname: Liu
mesh_terms: D000890:Anti-Infective Agents / Q000378:metabolism; D029769:Camptotheca / Q000382:microbiology*; D002166:Camptothecin / Q000031:analogs & derivatives / Q000032:analysis; D002853:Chromatography, Liquid; D002855:Chromatography, Thin Layer; D003470:Culture Media / Q000737:chemistry; D060026:Endophytes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D005658:Fungi / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D053719:Tandem Mass Spectrometry
chemical_list: D000890:Anti-Infective Agents; D003470:Culture Media; D002166:Camptothecin