title: A variety of mild stresses upregulate stanniocalcin-1 (STC-1) and induce mitohormesis in neural crest-derived cells.
abstract: We induced upregulation of stanniocalcin-1 (STC-1) by various mild and long lasting stresses and assayed its influence on mitochondrial membrane potential (MMP) in the neural crest-derived cell line Paju. The obtained data showed that starvation (24-96 h), exposure to 10nM TPA, and low concentrations (0.05-1 μM) of As2O3 significantly (3-5 times) upregulated Paju cell STC-1 RNA and stabilized the mitochondrial membrane potential (MMP). However, high concentrations of As2O3 (2.5-5.0 μM) increased intracellular ROS and free calcium levels and, consequently, suppressed STC-1 and MMP. The results show that cells preconditioned by various mild stresses expressed more STC-1 and their MMP were more resistant to a secondary exposure to As2O3 (2.5-5 μM, 96 h) demonstrating mitohormesis. We suggest that MMP deviation from control levels, to an extent innocuous to cell viability, is a general signal for STC-1-induction and MMP-protection. Our findings of Paju cell MMP-regulation may be of great importance for inventing new ways to prevent neurodegenerative diseases and unravel the mechanisms behind drug resistance.
authors.affiliation: Department of Stem Cell Biology, State Research Institute Centre for Innovative Medicine, Vilnius, Lithuania. daibironai@gmail.com
authors.forename: Daiva
authors.initials: D
authors.lastname: Bironaite
authors.forename: Johan Anders
authors.initials: JA
authors.lastname: Westberg
authors.forename: Leif Christer
authors.initials: LC
authors.lastname: Andersson
authors.forename: Algirdas
authors.initials: A
authors.lastname: Venalis
mesh_terms: D000310:Adrenal Gland Neoplasms / Q000473:pathology; D000970:Antineoplastic Agents / Q000494:pharmacology; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D059165:Hormesis / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects / Q000502:physiology*; D009447:Neuroblastoma / Q000473:pathology; D010087:Oxides / Q000494:pharmacology; D010703:Phorbol Esters / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism; D013217:Starvation; D013312:Stress, Physiological / Q000502:physiology*; D013997:Time Factors; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology*
chemical_list: D000970:Antineoplastic Agents; D001152:Arsenicals; D006023:Glycoproteins; D010087:Oxides; D010703:Phorbol Esters; D017382:Reactive Oxygen Species; C051795:teleocalcin; C032974:12-O-retinoylphorbol-13-acetate; D000077237:Arsenic Trioxide; D002118:Calcium