title: Mortality of therapeutic fish Garra rufa caused by Aeromonas sobria.
abstract: OBJECTIVE
  To investigate a case of mass mortality of Garra rufa (G. rufa) from a fish hatchery farm in Slovakia.
  
  
  METHODS
  Causative bacterial agent was swabbing out of affected fish skin area and subsequently identified using commercial test system. Antibiotic susceptibility was determined by the disk diffusion method.
  
  
  RESULTS
  Infected G. rufa was characterized by abnormal swimming behaviour, bleeding of skin lesions and local haemorrhages. Despite of using recommended aquatic antibiotic treatment no improvement was achieved and Aeromonas sobria (A. sobria) was identified as a causative agent of fish mortality. Due to massive fish mortality, antibiotic susceptibility of pure isolated culture of A. sobria was evaluated employing eight antibiotics against human infections. A. sobria was resistant only against one antibiotic, namely ampicilin.
  
  
  CONCLUSIONS
  These results indicate that A. sobria can act as a primary pathogen of G. rufa and may be a potential risk factor for immunodeficient or immunoincompetent patients during the ichthyotherapy.
authors.affiliation: Institute of Zoology, Slovak Academy of Sciences, Dubravska cesta 984 506 Bratislava, Slovakia ; Department of Microbiology, Faculty of Medicine, Slovak Medical University, Limbova 1 283 303, Bratislava, Slovakia.
authors.forename: Juraj
authors.initials: J
authors.lastname: Majtán
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Cerny
authors.forename: Alena
authors.initials: A
authors.lastname: Ofúkaná
authors.forename: Peter
authors.initials: P
authors.lastname: Takáč
authors.forename: Milan
authors.initials: M
authors.lastname: Kozánek
mesh_terms: D000333:Aeromonas / Q000187:drug effects* / Q000472:pathogenicity; D000667:Ampicillin / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015373:Bacterial Typing Techniques; D003530:Cyprinidae / Q000382:microbiology*; D024881:Drug Resistance, Bacterial; D005393:Fish Diseases / Q000188:drug therapy* / Q000382:microbiology / Q000401:mortality*; D005398:Fisheries; D008826:Microbial Sensitivity Tests; D018154:Slovakia
chemical_list: D000900:Anti-Bacterial Agents; D000667:Ampicillin
keywords: Aeromonas sobria; Antibiotic susceptibility; Antibiotic treatment; Garra rufa; Ichthyotherapy