title: HPLC analysis of camptothecin content in various parts of Nothapodytes foetida collected on different periods.
abstract: OBJECTIVE
  To investigate the content of topoisomerase I-DNA inhibitor alkaloid camptothecin (CPT) from various parts of Nothapodytes foetida (N. foetida) collected from the month of October to February.
  
  
  METHODS
  The content of CPT was quantified in the methanolic extract of various parts of N. foetida using high performance liquid chromatography (HPLC). Quantification was performed with the regression analysis and the method was validated as per ICH guidelines.
  
  
  RESULTS
  The results revealed that maximum concentrations of camptothecin were found in root (2.62%) collected in the month of February followed by fruits (January, 1.22%), stem (January, 0.81%) and leaves (February, 0.70%). Roots were found to have 3-fold higher concentration of CPT than the leaves and stem, while the fruits showed 2-fold higher concentration. Maximum concentration of camptothecin in fruits was observed in month of January, when they were not fully mature, which was 2-fold higher than that of young and fully mature fruits.
  
  
  CONCLUSIONS
  These findings indicate that the synthesis of CPT differs in different parts of N. foetida and the content varies periodically.
authors.affiliation: Department of Pharmacognosy, Poona College of Pharmacy, Bharati Vidyapeeth University, Pune, Maharashtra - 411038, India.
authors.forename: A G
authors.initials: AG
authors.lastname: Namdeo
authors.forename: A
authors.initials: A
authors.lastname: Sharma
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002166:Camptothecin / Q000302:isolation & purification / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005638:Fruit / Q000737:chemistry; D006801:Humans; D019684:Magnoliopsida / Q000737:chemistry*; D008517:Phytotherapy / Q000379:methods; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D012621:Seasons
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D002166:Camptothecin
keywords: Camptothecin; HPLC; Icacinaceae; Nothapodytes foetida