title: Promotion of neuronal differentiation of neural progenitor cells by using EGFR antibody functionalized collagen scaffolds for spinal cord injury repair.
abstract: The main challenge for neural progenitor cell (NPC)-mediated repair of spinal cord injury (SCI) is lack of favorable environment to direct its differentiation towards neurons rather than glial cells. The myelin associated inhibitors have been demonstrated to promote NPC differentiation into glial lineage. Herein, to inhibit the downstream signaling activated by myelin associated inhibitors, cetuximab, an epidermal growth factor receptor (EGFR) neutralizing antibody, functionalized collagen scaffold has been developed as a vehicle for NPC implantation. It was found that collagen-cetuximab 1 μg scaffolds enhanced neuronal differentiation and inhibited astrocytic differentiation of NPCs exposed to myelin proteins significantly in vitro. To test the therapeutic effect in vivo, NPCs expressing green fluorescent protein (GFP)-embedded scaffolds have been implanted into the 4 mm-long hemisection lesion of rats. We found that the collagen-cetuximab 5 μg scaffolds induced neuronal differentiation and decreased astrocytic differentiation of NPCs, enhanced axon regeneration, and promoted functional recovery markedly. A well-functionalized scaffold was constructed to improve the recovery of SCI, which could promote the neuronal differentiation of neural progenitor cells in vivo.
authors.affiliation: Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China.
authors.forename: Xiaoran
authors.initials: X
authors.lastname: Li
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Xiao
authors.forename: Jin
authors.initials: J
authors.lastname: Han
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.forename: Hanshan
authors.initials: H
authors.lastname: Xiao
authors.forename: Fukai
authors.initials: F
authors.lastname: Ma
authors.forename: Xianglin
authors.initials: X
authors.lastname: Hou
authors.forename: Xing
authors.initials: X
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Sun
authors.forename: Wenyong
authors.initials: W
authors.lastname: Ding
authors.forename: Yannan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Bing
authors.initials: B
authors.lastname: Chen
authors.forename: Jianwu
authors.initials: J
authors.lastname: Dai
mesh_terms: D000818:Animals; D000906:Antibodies / Q000494:pharmacology*; D061067:Antibodies, Monoclonal, Humanized / Q000494:pharmacology; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002452:Cell Count; D002454:Cell Differentiation / Q000187:drug effects*; D000068818:Cetuximab; D003094:Collagen / Q000494:pharmacology*; D066246:ErbB Receptors / Q000037:antagonists & inhibitors / Q000276:immunology*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D051379:Mice; D058953:Neural Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000637:transplantation; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function / Q000187:drug effects; D013119:Spinal Cord Injuries / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D058630:Spinal Cord Regeneration / Q000187:drug effects; D033581:Stem Cell Transplantation; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D000906:Antibodies; D061067:Antibodies, Monoclonal, Humanized; D049452:Green Fluorescent Proteins; D003094:Collagen; D066246:ErbB Receptors; D000068818:Cetuximab