title: The sld genetic defect: two intronic CA repeats promote insertion of the subsequent intron and mRNA decay.
abstract: The autosomal recessive mutation, sld, attenuates mucous cell expression in murine sublingual glands with corresponding effects on mucin 19 (Muc19). We conducted a systematic study including genetic mapping, sequencing, and functional analyses to elucidate a mutation to explain the sld phenotype in neonatal mice. Genetic mapping and gene expression analyses localized the sld mutation within the gene Muc19/Smgc, specifically attenuating Muc19 transcripts, and Muc19 knock-out mice mimic the sld phenotype in neonates. Muc19 transcription is unaffected in sld mice, whereas mRNA stability is markedly decreased. Decreased mRNA stability is not due to a defect in 3'-end processing nor to sequence differences in Muc19 transcripts. Comparative sequencing of the Muc19/Smgc gene identified four candidate intronic mutations within the Muc19 coding region. Minigene splicing assays revealed a novel splicing event in which insertion of two additional repeats within a CA repeat region of intron 53 of the sld genome enhances retention of intron 54, decreasing the levels of correctly spliced transcripts. Moreover, pateamine A, an inhibitor of nonsense-mediated mRNA decay, inhibits degradation of aberrant Muc19 transcripts. The mutation in intron 53 thus enhances aberrant splicing leading to degradation of aberrant transcripts and decreased Muc19 message stability, consistent with the sld phenotype. We propose a working model of the unique splicing event enhanced by the mutation, as well as putative explanations for the gradual but limited increase in Muc19 glycoprotein expression and its restricted localization to subpopulations of mucous cells in sld mice during postnatal gland development.
authors.affiliation: Department of Oral Biology, College of Dentistry, University of Florida, Gainesville, Florida 32610, USA.
authors.forename: Biswadip
authors.initials: B
authors.lastname: Das
authors.forename: Melanie N
authors.initials: MN
authors.lastname: Cash
authors.forename: Bently
authors.initials: B
authors.lastname: Robinson
authors.forename: Christopher S
authors.initials: CS
authors.lastname: Kuhns
authors.forename: Lisa R
authors.initials: LR
authors.lastname: Latchney
authors.forename: Margaret A
authors.initials: MA
authors.lastname: Fallon
authors.forename: Rosemary W
authors.initials: RW
authors.lastname: Elliott
authors.forename: Arthur R
authors.initials: AR
authors.lastname: Hand
authors.forename: David J
authors.initials: DJ
authors.lastname: Culp
mesh_terms: D017398:Alternative Splicing / Q000502:physiology; D000818:Animals; D005786:Gene Expression Regulation / Q000502:physiology; D007438:Introns / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D008954:Models, Biological; D009077:Mucins / Q000096:biosynthesis* / Q000235:genetics; D009154:Mutation; D016366:Open Reading Frames / Q000502:physiology; D020871:RNA Stability / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D013361:Sublingual Gland / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*
chemical_list: C482348:Muc19 protein, mouse; D009077:Mucins; D012333:RNA, Messenger
keywords: Gene Mapping; Muc19; Mucins; Mutant; Nonsense-mediated Decay; RNA Processing; RNA Splicing