title: Immunologic evaluation and validation of methods using synthetic peptides derived from Mycobacterium tuberculosis for the diagnosis of tuberculosis infection.
abstract: The goal of this study was to demonstrate the usefulness of an enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of pulmonary tuberculosis (PTB) and extrapulmonary TB (EPTB). This assay used 20 amino acid-long, non-overlapped synthetic peptides that spanned the complete Mycobacterium tuberculosis ESAT-6 and Ag85A sequences. The validation cohort consisted of 1,102 individuals who were grouped into the following five diagnostic groups: 455 patients with PTB, 60 patients with EPTB, 40 individuals with non-EPTB, 33 individuals with leprosy and 514 healthy controls. For the PTB group, two ESAT-6 peptides (12033 and 12034) had the highest sensitivity levels of 96.9% and 96.2%, respectively, and an Ag85A-peptide (29878) was the most specific (97.4%) in the PTB groups. For the EPTB group, two Ag85A peptides (11005 and 11006) were observed to have a sensitivity of 98.3% and an Ag85A-peptide (29878) was also the most specific (96.4%). When combinations of peptides were used, such as 12033 and 12034 or 11005 and 11006, 99.5% and 100% sensitivities in the PTB and EPTB groups were observed, respectively. In conclusion, for a cohort that consists entirely of individuals from Venezuela, a multi-antigen immunoassay using highly sensitive ESAT-6 and Ag85A peptides alone and in combination could be used to more rapidly diagnose PTB and EPTB infection.
authors.affiliation: Laboratorio de Inmunología de Enfermedades Infecciosas, Instituto de Biomedicina, Universidad Central de Venezuela, Caracas, Venezuela. zaraujogarcia@yahoo.com
authors.forename: Zaida
authors.initials: Z
authors.lastname: Araujo
authors.forename: Francesca
authors.initials: F
authors.lastname: Giampietro
authors.forename: María de los Angeles
authors.initials: Mde L
authors.lastname: Bochichio
authors.forename: Andrea
authors.initials: A
authors.lastname: Palacios
authors.forename: Jenifer
authors.initials: J
authors.lastname: Dinis
authors.forename: Jaime
authors.initials: J
authors.lastname: Isern
authors.forename: Jacobus Henry de
authors.initials: JH
authors.lastname: Waard
authors.forename: Elsa
authors.initials: E
authors.lastname: Rada
authors.forename: Rafael
authors.initials: R
authors.lastname: Borges
authors.forename: Carlos
authors.initials: C
authors.lastname: Fernández de Larrea
authors.forename: Angel
authors.initials: A
authors.lastname: Villasmil
authors.forename: Magnolia
authors.initials: M
authors.lastname: Vanegas
authors.forename: Jose Antonio
authors.initials: JA
authors.lastname: Enciso-Moreno
authors.forename: Manuel Alfonso
authors.initials: MA
authors.lastname: Patarroyo
mesh_terms: D000328:Adult; D000942:Antigens, Bacterial / Q000276:immunology; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D010455:Peptides / Q000276:immunology; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000175:diagnosis* / Q000276:immunology; D014397:Tuberculosis, Pulmonary / Q000175:diagnosis / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D010455:Peptides