title: [Identification of a novel HLA allele HLA-B*55:35].
abstract: OBJECTIVE
  To identify a novel human leukocyte antigen (HLA) B allele and explore its family heritage.
  
  
  METHODS
  A novel HLA allele was suspected upon routine HLA typing using a polymerase chain reaction-sequence specific oligonucleotide probe (PCR-SSOP) assay. The sequence was confirmed with DNA sequencing and compared with its closest matching allele, B*55:02. The family was also investigated.
  
  
  RESULTS
  An unusual reaction pattern was detected during routine HLA typing. The sequence was confirmed to be a novel HLA-B allele, which differed from the closest matching allele, B*55:02 in 7 nt positions in exon 2. Among the 7 mutations from 6 codons, there were two amino acids changes including 69Glu→Met and 70Glu→Ala.
  
  
  CONCLUSIONS
  A novel HLA-B allele has been identified and officially named as B*55:35 by the WHO Nomenclature Committee for Factors of the HLA System (GenBank accession number FJ898284).
authors.affiliation: China Marrow Donor Program HLA Typing Laboratory, Blood Center of Shandong Province, Jinan, P.R. China.
authors.forename: Yong-hong
authors.initials: YH
authors.lastname: Song
authors.forename: Yong-xin
authors.initials: YX
authors.lastname: Mao
authors.forename: Kui
authors.initials: K
authors.lastname: Dong
authors.forename: Xiao-jian
authors.initials: XJ
authors.lastname: Chen
authors.forename: Yan-xia
authors.initials: YX
authors.lastname: Gu
authors.forename: Chuan-fu
authors.initials: CF
authors.lastname: Zhu
mesh_terms: D000483:Alleles; D001483:Base Sequence; D015235:HLA-B Antigens / Q000235:genetics*; D006650:Histocompatibility Testing; D006801:Humans; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA
chemical_list: D015235:HLA-B Antigens; C082944:HLA-B55 antigen