title: Analysis of chemical composition and bioactive property evaluation of Indian propolis.
abstract: OBJECTIVE
  To analyze the chemical composition and to evaluate the bioactive potential of hydroalocoholic extract of propolis.
  
  
  METHODS
  Ethanol extract of propolis was analyzed by GC-MS, HPTLC and HPLC methods and in vitro antioxidant, anticholinesterase and cytotoxicity assay were performed.
  
  
  RESULTS
  GC-MS analysis revealed the presence of fatty acids, alcohols, and quercetin. Quercetin was identified and quantified by HPTLC and HPLC methods. Dose dependent DPPH and hydroxyl radical scavenging activity of hydroalcoholic extract of propolis was calculated as 16.20 and 34.33 µg/mL respectively. Inhibition of lipid peroxidation was significant and the IC50 value was calculated as 55.56µg/mL. Anticholinesterase activity was less observed. The cytotoxic activity against both breast (MCF-7) and lung cancer (A543) cell lines were significant and the IC50 value was calculated as 10 and 13 µg/mL respectively.
  
  
  CONCLUSIONS
  These findings showed that bioactive compounds present in propolis will alleviate many diseases and can be used for better human health.
authors.affiliation: PG and Research Department of Biotechnology, Kongunadu Arts and Science College, GN Mills, Coimbatore-029, Tamil nadu, India.
authors.forename: R
authors.initials: R
authors.lastname: Thirugnanasampandan
authors.forename: Sayana Beena
authors.initials: SB
authors.lastname: Raveendran
authors.forename: R
authors.initials: R
authors.lastname: Jayakumar
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000494:pharmacology; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007194:India; D020128:Inhibitory Concentration 50; D011429:Propolis / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D002800:Cholinesterase Inhibitors; D005227:Fatty Acids; D016166:Free Radical Scavengers; C013584:lipid peroxidation inhibitor; D011429:Propolis
keywords: Anticholinesterase; Antioxidant; Cytotoxicity; Densitometry; Propolis; Quercetin