title: [Frequencies of 9 bp deletion of mitochondrial DNA in ethnic Miao, Buyi and Dong from Guizhou].
abstract: OBJECTIVE
  To study the frequency of a 9 bp deletion polymorphism of mitochondrial DNA (mtDNA) in ethnic Miao, Buyi and Dong populations from Guizhou province.
  
  
  METHODS
  Polymerase chain reaction-polyacrylamide gel electrophoresis (PCR-PAGE) was used to detect the 9 bp deletion. The result was verified with DNA sequencing.
  
  
  RESULTS
  Two polymorphisms, including a standard pattern and a short pattern (the 9 bp deletion), were found among the three ethnic groups. The frequency of short pattern in 304 males was 23.0%. Respectively, those of Miao, Buyi and Dong ethnics were 28.6%, 26.8% and 13.7%. A statistically significant difference was detected among the three groups (P<0.05).
  
  
  CONCLUSIONS
  The frequencies of the 9 bp polymorphism were relatively high among ethnic Miao, Buyi and Dong populations from Guizhou, and there was a significant difference between the three.
authors.affiliation: Key Laboratory of Molecular Biology, Guiyang Medical University, Guizhou 550004, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: He
authors.forename: Ke-ren
authors.initials: KR
authors.lastname: Shan
authors.forename: Lin-yan
authors.initials: LY
authors.lastname: Ren
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.forename: Chan-juan
authors.initials: CJ
authors.lastname: Wang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Xie
authors.forename: Chan
authors.initials: C
authors.lastname: Zhang
authors.forename: Lin-jie
authors.initials: LJ
authors.lastname: Li
authors.forename: Zhi-zhong
authors.initials: ZZ
authors.lastname: Guan
mesh_terms: D001483:Base Sequence; D002681:China / Q000208:ethnology; D004272:DNA, Mitochondrial / Q000235:genetics*; D017353:Gene Deletion; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D004272:DNA, Mitochondrial