title: Mesoporous silicas synthesis and application for lignin peroxidase immobilization by covalent binding method.
abstract: Immobilization of enzymes on mesoporous silicas (MS) allows for good reusability. MS with two-dimensional hexagonal pores in diameter up to 14.13 nm were synthesized using Pluronic P123 as template and 1,3,5-triisopropylbenzene as a swelling agent in acetate buffer. The surface of MS was modified by the silanization reagents 3-aminopropyltriethoxysilane. Lignin peroxidase (LiP) was successfully immobilized on the modified MS through covalent binding method by four agents: glutaraldehyde, 1,4-phenylene diisothiocyanate, cyanotic chloride and water-soluble carbodiimide. Results showed that cyanotic chloride provided the best performance for LIP immobilization. The loaded protein concentration was 12.15 mg/g and the immobilized LiP activity was 812.9 U/L. Immobilized LiP had better pH stability. Acid Orange II was used to examine the reusability of immobilized LiP, showing more than 50% of the dye was decolorized at the fifth cycle.
authors.affiliation: Environmental Simulation and Pollution Control State Key Joint Laboratory, School of Environment, Tsinghua University, Beijing 100084, China. hzf07@mails.tsinghua.edu.cn
authors.forename: Zunfang
authors.initials: Z
authors.lastname: Hu
authors.forename: Longqian
authors.initials: L
authors.lastname: Xu
authors.forename: Xianghua
authors.initials: X
authors.lastname: Wen
mesh_terms: D002234:Carbodiimides / Q000737:chemistry; D004800:Enzymes, Immobilized; D005976:Glutaral / Q000737:chemistry; D010544:Peroxidases / Q000737:chemistry*; D011060:Poloxalene / Q000138:chemical synthesis; D016062:Porosity; D011437:Propylamines; D012821:Silanes / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D002234:Carbodiimides; D004800:Enzymes, Immobilized; D011437:Propylamines; D012821:Silanes; C464484:pluronic block copolymer P123; D012822:Silicon Dioxide; D011060:Poloxalene; D010544:Peroxidases; C042858:lignin peroxidase; C477625:amino-propyl-triethoxysilane; D005976:Glutaral