title: Evaluation of in-vitro antibacterial activity and anti-inflammatory activity for different extracts of Rauvolfia tetraphylla L. root bark.
abstract: OBJECTIVE
  To assess the in-vitro antibacterial activity and anti-inflammatory activity of orally administered different extracts (Hydro-alcoholic, methanolic, ethyl acetate and hexane) of Rauvolfia tetraphylla (R. tetraphylla) root bark in Carrageenan induced acute inflammation in rats.
  
  
  METHODS
  In-vitro antibacterial activity was evaluated for extracts against four Gram positive and four Gram negative bacteria by using cylinder plate assay. Hydro-alcoholic extract (70% v/v ethanol) at 200, 400 and 800 mg/kg doses and methanolic, ethyl acetate and hexane extracts at doses 100, 200 and 400 mg/kg were tested for anti-inflammatory activity in Carrageenan induced rat paw oedema model and paw thickness was measured every one hour up to 6 hrs.
  
  
  RESULTS
  All extracts of R. tetraphylla root bark showed good zone of inhibition against tested bacterial strains. In Carrageenan induced inflammation model, hydro-alcoholic and methanolic extract of R. tetraphylla root bark at three different doses produced significant (P<0.001) reduction when compared to vehicle treated control group and hexane, ethyl acetate extracts.
  
  
  CONCLUSIONS
  In the present study extracts of R. tetraphylla root bark shows good in-vitro antibacterial activity and in-vivo anti-inflammatory activity in rats.
authors.affiliation: A.U College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, A.P, India-530003.
authors.forename: B
authors.initials: B
authors.lastname: Ganga Rao
authors.forename: P
authors.initials: P
authors.lastname: Umamaheswara Rao
authors.forename: E
authors.initials: E
authors.lastname: Sambasiva Rao
authors.forename: T
authors.initials: T
authors.lastname: Mallikarjuna Rao
authors.forename: V S
authors.initials: VS
authors.lastname: Praneeth D
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D002351:Carrageenan / Q000009:adverse effects; D004195:Disease Models, Animal; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D005260:Female; D008297:Male; D008826:Microbial Sensitivity Tests; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry*; D051381:Rats; D011926:Rauwolfia / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D002351:Carrageenan
keywords: Anti-inflammatory activity; In-vitro antibacterial activity; Inflammation; Rauvolfia tetraphylla; Root bark