title: Identification and characterization of the actinomycin G gene cluster in Streptomyces iakyrus.
abstract: The gene cluster directing actinomycin G biosynthesis in Streptomyces iakyrus has been identified and sequenced. It contains one actinomycin synthetase I (ACMS I) gene and two copies of ACMS II and III genes. Genetic analysis demonstrates a unique partnership between the putative hydroxylation and chlorination activities as both acmG8 and acmG9 genes need to be transcribed for the biosynthesis of actinomycin G2-3, respectively.
authors.affiliation: Marine Biodiscovery Centre, Department of Chemistry, University of Aberdeen, Aberdeen AB24 3LZ, Scotland, UK.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Wang
authors.forename: Jioji
authors.initials: J
authors.lastname: Tabudravu
authors.forename: Mostafa Ezzat
authors.initials: ME
authors.lastname: Rateb
authors.forename: Krystal Joan
authors.initials: KJ
authors.lastname: Annand
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Qin
authors.forename: Marcel
authors.initials: M
authors.lastname: Jaspars
authors.forename: Zixin
authors.initials: Z
authors.lastname: Deng
authors.forename: Yi
authors.initials: Y
authors.lastname: Yu
authors.forename: Hai
authors.initials: H
authors.lastname: Deng
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis*; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D003609:Dactinomycin / Q000096:biosynthesis*; D005798:Genes, Bacterial; D054879:Halogenation; D006900:Hydroxylation; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000235:genetics*; D005810:Multigene Family; D009713:Nucleotidyltransferases / Q000235:genetics*; D010453:Peptide Synthases / Q000235:genetics*; D013302:Streptomyces / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D009097:Multienzyme Complexes; C586385:actinomycin G; D003609:Dactinomycin; D009713:Nucleotidyltransferases; C075125:actinomycin synthetase I; D010453:Peptide Synthases; C052258:actinomycin synthetase II; C052259:actinomycin synthetase III