title: MicroRNA-107 inhibits U87 glioma stem cells growth and invasion.
abstract: Glioma stem cells (GSCs) are thought to be critical for resistance to radiotherapy and chemotherapy and for tumor recurrence after surgery in glioma patients. Identification of new therapeutic strategies that can target GSCs may thus be critical for improving patient survival. MicroRNAs (miRNAs) are small non-coding RNAs that function as tumor suppressors or oncogenes. In this study, we confirmed that miR-107 was down-regulated in GSCs. To investigate the role of miR-107 in tumorigenesis of GSCs, a lentiviral vector over-expressing miR-107 in U87GSCs was constructed. We found that over-expression of miR-107 suppressed proliferation and down-regulated Notch2 protein and stem cell marker (CD133 and Nestin) expression in U87GSCs. Furthermore, enhanced miR-107 expression significantly inhibited U87GSC invasion and reduced matrix metalloproteinase-12 expression. miR-107 also suppressed U87GSCs xenograft growth in vivo. These findings suggest that miR-107 is involved in U87GSCs growth and invasion and may provide a potential therapeutic target for glioma treatment.
authors.affiliation: The National Key Clinic Specialty, Guangdong Provincial Key Laboratory on Brain Function Repair and Regeneration, Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.forename: Xiang-rong
authors.initials: XR
authors.lastname: Chen
authors.forename: Fan-fan
authors.initials: FF
authors.lastname: Chen
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.forename: Run
authors.initials: R
authors.lastname: Zhang
authors.forename: Ke
authors.initials: K
authors.lastname: Yan
authors.forename: Yong-jun
authors.initials: YJ
authors.lastname: Yi
authors.forename: Zhi-min
authors.initials: ZM
authors.lastname: Xu
authors.forename: Xiao-Dan
authors.initials: XD
authors.lastname: Jiang
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D001483:Base Sequence; D001932:Brain Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000201:enzymology / Q000235:genetics* / Q000473:pathology*; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D016086:Lentivirus / Q000378:metabolism; D053508:Matrix Metalloproteinase 12 / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009361:Neoplasm Invasiveness; D014411:Neoplastic Stem Cells / Q000201:enzymology / Q000473:pathology*; D064231:Nestin / Q000235:genetics / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D051883:Receptor, Notch2 / Q000235:genetics / Q000378:metabolism; D014161:Transduction, Genetic; D015854:Up-Regulation / Q000235:genetics; D023041:Xenograft Model Antitumor Assays
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; C549045:MIRN107 microRNA, human; D035683:MicroRNAs; D064231:Nestin; C000606023:PROM1 protein, human; D010455:Peptides; C000606024:Prom1 protein, mouse; D051883:Receptor, Notch2; C507122:MMP12 protein, human; D053508:Matrix Metalloproteinase 12