title: Mutate Chlorella sp. by nuclear irradiation to fix high concentrations of CO2.
abstract: To improve biomass productivity and CO2 fixation of microalgae under 15% (v/v) CO2 of flue gas, Chlorella species were mutated by nuclear irradiation and domesticated with high concentrations of CO2. The biomass yield of Chlorella pyrenoidosa mutated using 500 Gy of (60)Co γ irradiation increased by 53.1% (to 1.12 g L(-1)) under air bubbling. The mutants were domesticated with gradually increased high concentrations of CO2 [from 0.038% (v/v) to 15% (v/v)], which increased the biomass yield to 2.41 g L(-1). When light transmission and culture mixing in photo-bioreactors were enhanced at 15% (v/v) CO2, the peak growth rate of the domesticated mutant (named Chlorella PY-ZU1) was increased to 0.68 g L(-1) d(-1). When the ratio of gas flow rate (L min(-1)) to 1L of microalgae culture was 0.011, the peak CO2 fixation rate and the efficiency of Chlorella PY-ZU1 were 1.54 g L(-1) d(-1) and 32.7%, respectively.
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China. juncheng@zju.edu.cn
authors.forename: Jun
authors.initials: J
authors.lastname: Cheng
authors.forename: Yun
authors.initials: Y
authors.lastname: Huang
authors.forename: Jia
authors.initials: J
authors.lastname: Feng
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.forename: Junhu
authors.initials: J
authors.lastname: Zhou
authors.forename: Kefa
authors.initials: K
authors.lastname: Cen
mesh_terms: D018533:Biomass; D019149:Bioreactors; D057486:Carbon Cycle / Q000187:drug effects* / Q000528:radiation effects; D002245:Carbon Dioxide / Q000494:pharmacology*; D002467:Cell Nucleus / Q000528:radiation effects*; D002708:Chlorella / Q000187:drug effects* / Q000254:growth & development / Q000528:radiation effects*; D003037:Cobalt Radioisotopes; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D006863:Hydrogen-Ion Concentration / Q000187:drug effects / Q000528:radiation effects; D009154:Mutation / Q000235:genetics*
chemical_list: D003037:Cobalt Radioisotopes; D002245:Carbon Dioxide