title: Identification and differential expression of human carcinoma-associated antigens in hepatocellular carcinoma tissues.
abstract: This study was designed to identify and verify hepatocellular carcinoma (HCC)-associated human carcinoma antigens (HCAs) that may be useful as tumor markers for HCC. We found that BCE075 and BCD021 anti-HCA antibodies were immunostained in the liver tissue samples and showed specific staining. Their expression was increased in HCC compared with normal liver tissues (P = 0.008). Immunoprecipitation and mass spectrometry analyses of the proteins precipitated by these two antibodies were identified to be cytoskeleton-associated protein 4 (CLIMP63) and brain-type glycogen phosphorylase (PYGB). This study demonstrated that HCC tissues expressed specific HCA glycoproteins, suggesting that our mouse monoclonal anti-HCA antibodies could be useful for immunohistochemical analysis of HCA expression as potential biomarkers for HCC diagnosis.
authors.affiliation: Department of Hepatobiliary Disease I, Eastern Hepatobiliary Surgery Hospital, The Second Military Medical University, 225 Changhai Road, Shanghai 200438, China.
authors.forename: Dong-Xun
authors.initials: DX
authors.lastname: Zhou
authors.forename: Long-Yi
authors.initials: LY
authors.lastname: Zheng
authors.forename: Hai-xia
authors.initials: HX
authors.lastname: Qu
authors.forename: Feng-hai
authors.initials: FH
authors.lastname: Yu
authors.forename: Shan-shan
authors.initials: SS
authors.lastname: Zou
authors.forename: Yi
authors.initials: Y
authors.lastname: Hong
authors.forename: Ye-Xiong
authors.initials: YX
authors.lastname: Tan
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: He-Ping
authors.initials: HP
authors.lastname: Hu
authors.forename: Hong-Yang
authors.initials: HY
authors.lastname: Wang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm / Q000032:analysis*; D014408:Biomarkers, Tumor; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D025002:Glycogen Phosphorylase, Brain Form / Q000032:analysis*; D006801:Humans; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D008113:Liver Neoplasms / Q000473:pathology*; D013058:Mass Spectrometry; D008565:Membrane Proteins / Q000032:analysis*; D051379:Mice
chemical_list: D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; C081876:CKAP4 protein, human; D008565:Membrane Proteins; D025002:Glycogen Phosphorylase, Brain Form