title: Effect of acute tadalafil on sperm motility and acrosome reaction: in vitro and in vivo studies.
abstract: Effects of acute tadalafil on sperm motility and acrosome reaction were investigated, both in vitro and in vivo. Twenty asthenozoospermic and 20 normozoospermic patients as control were randomly enrolled. For in vitro part, 0.5 ml tadalafil solutions with different concentrations were added (0.2, 0.1, 0.05 and 0.025 μg ml(-1) , respectively) into semen samples. In both groups, samples treated with 0.2 μg ml(-1) tadalafil had significant increase in sperm motility after 2 h incubation. For in vivo part, oral administration of tadalafil (20 mg) or sildenafil (100 mg) was given. In both groups, computer-assisted semen analysis parameters showed no significant difference. After the administration of tadalafil (2 h) and sildenafil (1 h), there was no significant difference observed in premature acrosome reaction incidence rate. Taking both in vitro and in vivo results into consideration, acute on-demand administration of tadalafil would have no adverse effect on semen parameters.
authors.affiliation: Department of Urology, Shanghai Institute of Andrology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Yang
authors.forename: Y
authors.initials: Y
authors.lastname: Ma
authors.forename: H
authors.initials: H
authors.lastname: Yang
authors.forename: Y
authors.initials: Y
authors.lastname: Jin
authors.forename: K
authors.initials: K
authors.lastname: Hu
authors.forename: H-X
authors.initials: HX
authors.lastname: Wang
authors.forename: Y-X
authors.initials: YX
authors.lastname: Wang
authors.forename: Y-R
authors.initials: YR
authors.lastname: Huang
authors.forename: B
authors.initials: B
authors.lastname: Chen
mesh_terms: D020101:Acrosome Reaction / Q000187:drug effects; D000328:Adult; D053627:Asthenozoospermia / Q000188:drug therapy; D002243:Carbolines / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D008297:Male; D010879:Piperazines / Q000494:pharmacology; D011687:Purines / Q000494:pharmacology; D055101:Semen Analysis; D000068677:Sildenafil Citrate; D013081:Sperm Motility / Q000187:drug effects; D013450:Sulfones / Q000494:pharmacology; D000068581:Tadalafil
chemical_list: D002243:Carbolines; D010879:Piperazines; D011687:Purines; D013450:Sulfones; D000068581:Tadalafil; D000068677:Sildenafil Citrate
keywords: Acrosome reaction; PDE5 inhibitor; semen parameters; tadalafil