title: Stable gene-silence of Kif2a synergistic with 5-fluorouracil suppresses oral tongue squamous cell carcinoma growth in vitro and in vivo.
abstract: OBJECTIVE
  Squamous cell carcinoma of the oral tongue (SCCOT) is one of the most common malignant carcinomas in the head and neck. Recurrence and/or metastasis often results in failure of treatment and decreases the survival of the patients. The purpose of this study is to investigate the effect of gene-silence of Kif2a on SCCOT in viro and in vivo.
  
  
  METHODS
  Plasmid-mediated expression of Kif2a-siRNA (pGPU6/GFP/Kif2a) was employed to silence the expression of Kif2a in Tca8113 cells at both mRNA and protein levels. Tca8113 cell proliferation was measured by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay and growth of Tca8113 tumors was determined by intra-tumor injection of pGPU6/GFP/Kif2a in nude mice.
  
  
  RESULTS
  Gene-silence of Kif2a suppressed Tca8113 cell proliferation. pGPU6/GFP/Kif2a synergized the tumor suppression effect of 5-Fluorouracil (5-Fu) on Tca8113 cells.
  
  
  CONCLUSIONS
  Our data support that Kif2a is a potential molecular target for the therapeutics of recurrent and metastatic SCCOT.
authors.affiliation: Department of Pathology, Medical College of Qingdao University, Qingdao, Shandong, China.
authors.forename: Cheng-Qin
authors.initials: CQ
authors.lastname: Wang
authors.forename: Yu-Jun
authors.initials: YJ
authors.lastname: Li
authors.forename: Zhi-Min
authors.initials: ZM
authors.lastname: Wei
authors.forename: Chang-Jun
authors.initials: CJ
authors.lastname: Zhu
authors.forename: Xun
authors.initials: X
authors.lastname: Qu
authors.forename: Feng-Cai
authors.initials: FC
authors.lastname: Wei
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Xing
authors.forename: Wen-Juan
authors.initials: WJ
authors.lastname: Yu
mesh_terms: D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology*; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000628:therapy; D045744:Cell Line, Tumor; D005472:Fluorouracil / Q000494:pharmacology*; D020868:Gene Silencing; D006801:Humans; D016547:Kinesins / Q000235:genetics*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D010957:Plasmids; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D012097:Repressor Proteins / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014062:Tongue Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000628:therapy; D014162:Transfection
chemical_list: D000964:Antimetabolites, Antineoplastic; C509805:KIF2A protein, human; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D012097:Repressor Proteins; C477238:KIF2A protein, mouse; D016547:Kinesins; D005472:Fluorouracil