title: 7,8-Dihydroxy coumarin promotes chondrogenic differentiation of adipose-derived mesenchymal stem cells.
abstract: OBJECTIVE
  To investigate the effects of 7,8-dihydroxy coumarin on the chondrogenic differentiation of rat adipose-derived mesenchymal stem cells (ADMSCs).
  
  
  METHODS
  ADMSCs were cultured using a micromass suspension method and incubated with different concentrations of 7,8-dihydroxy coumarin and transforming growth factor (TGF)-β1 for 3 weeks: group A (negative control, no drug treatment); group B (positive control, 10 ng/ml TGF-β1); groups C, D and E (incubated with 25, 50 and 100 µg/ml 7,8-dihydroxy coumarin, respectively); groups F, G and H (incubated with 25, 50 and 100 µg/ml 7,8-dihydroxy coumarin, respectively, plus 10 ng/ml TGF-β1). Markers of chondrogenic differentiation were measured using histology, immunohistochemistry, enzyme-linked immunosorbent assay and reverse transcription-polymerase chain reaction.
  
  
  RESULTS
  When used alone, 7,8-dihydroxy coumarin only weakly induced the chondrogenic differentiation of ADMSCs. 7,8-Dihydroxy coumarin used in combination with TGF-β1 strongly induced chondrogenic differentiation of ADMSCs. For some of the markers of chondrogenic differentiation, the extent of the induction was 7,8-dihydroxy coumarin dose-dependent.
  
  
  CONCLUSIONS
  7,8-Dihydroxy coumarin appears to work synergistically with TGF-β1 to strongly induce chondrogenic differentiation of rat-derived ADMSCs.
authors.affiliation: Department of Plastic and Reconstructive Surgery, First Hospital of Jilin University, Changchun, Jilin Province, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Shao
authors.forename: Quan
authors.initials: Q
authors.lastname: Lin
authors.forename: Haipeng
authors.initials: H
authors.lastname: Liu
authors.forename: Duo
authors.initials: D
authors.lastname: Zhang
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D020219:Chondrogenesis / Q000187:drug effects*; D024043:Collagen Type II / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006025:Glycosaminoglycans / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling; D014468:Umbelliferones / Q000494:pharmacology*
chemical_list: D024043:Collagen Type II; D006025:Glycosaminoglycans; D012333:RNA, Messenger; D014468:Umbelliferones; C039952:daphnetin