title: A novel high-resolution melt PCR assay discriminates Anaplasma phagocytophilum and "Candidatus Neoehrlichia mikurensis".
abstract: "Candidatus Neoehrlichia mikurensis" (Anaplasmataceae) is an emerging pathogen transmitted by Ixodes ticks. Conventional PCR and the newly developed high-resolution melt PCR were used to detect and discriminate "Candidatus Neoehrlichia mikurensis" and Anaplasma phagocytophilum. Both bacterial species were frequently found in Ixodes ricinus and Ixodes hexagonus but virtually absent from Dermacentor reticulatus. In rodents, "Candidatus N. mikurensis" was significantly more prevalent than A. phagocytophilum, whereas in cats, only A. phagocytophilum was found.
authors.affiliation: Institute for Parasitology and Tropical Veterinary Medicine, Freie Universität Berlin, Berlin, Germany.
authors.forename: Jürgen
authors.initials: J
authors.lastname: Krücken
authors.forename: Cécile
authors.initials: C
authors.lastname: Schreiber
authors.forename: Denny
authors.initials: D
authors.lastname: Maaz
authors.forename: Mareen
authors.initials: M
authors.lastname: Kohn
authors.forename: Janina
authors.initials: J
authors.lastname: Demeler
authors.forename: Stefanie
authors.initials: S
authors.lastname: Beck
authors.forename: Eberhard
authors.initials: E
authors.lastname: Schein
authors.forename: Philipp
authors.initials: P
authors.lastname: Olias
authors.forename: Dania
authors.initials: D
authors.lastname: Richter
authors.forename: Franz-Rainer
authors.initials: FR
authors.lastname: Matuschka
authors.forename: Stefan
authors.initials: S
authors.lastname: Pachnicke
authors.forename: Klemens
authors.initials: K
authors.lastname: Krieger
authors.forename: Barbara
authors.initials: B
authors.lastname: Kohn
authors.forename: Georg
authors.initials: G
authors.lastname: von Samson-Himmelstjerna
mesh_terms: D041081:Anaplasma phagocytophilum; D000710:Anaplasmataceae / Q000145:classification* / Q000235:genetics*; D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods*; D003870:Dermacentor / Q000382:microbiology; D018884:Ixodes / Q000382:microbiology; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012377:Rodentia / Q000382:microbiology