title: S100A expression and interleukin-10 polymorphisms are associated with ulcerative colitis and diarrhea predominant irritable bowel syndrome.
abstract: BACKGROUND
  Both ulcerative colitis (UC) and diarrhea-predominant irritable bowel syndrome (IBS-D) are associated with alterations in enteric serotonin (5-HT) signaling.
  
  
  OBJECTIVE
  The purpose of this study was to compare the rectal and sigmoid colonic mucosal expression of S100A proteins and functional polymorphisms of the 5-HT transporter (5HTT) and interleukin-10 genes in patients with IBS-D or UC with healthy controls.
  
  
  METHODS
  mRNA expression of S100 proteins was measured in sigmoid and rectal biopsies and in rectal epithelium isolated by laser-captured microdissection. Leucocyte DNA was analyzed by PCR-based reaction fragment length polymorphisms and direct sequencing. Clinical symptoms were assessed by the self-rating depression scale and by the gastrointestinal symptom rating scale.
  
  
  RESULTS
  Fifty patients with IBS-D, 56 with UC and 50 healthy controls were studied. Colonic mucosal expression of S100A8 and S100A9 in UC was significantly higher than in IBS or controls and correlated with the UC disease activity index (r = 0.65, p < 0.001). S100A10 expression in the rectal epithelium of the IBS patients was significantly higher (0.643 vs. 0.402, p = 0.01) than in controls and correlated with the SDS scores (r = 0.41, p = 0.002). The frequency of IL10-819 CC genotype was significantly higher in IBS-D (10.7 vs. 0 %, p = 0.047) and UC (16 vs. 0 %, p = 0.007) than that in controls.
  
  
  CONCLUSIONS
  Overexpression of S100A10 in the rectum may play a role in IBS as it is involved in modulating 5-HT1B receptors. The IL10-819 CC is a candidate genotype for both IBS and UC in Japanese.
authors.affiliation: Department of Internal Medicine, Kawasaki Medical School, Okayama, Japan. shiotani@med.kawasaki-m.ac.jp
authors.forename: Akiko
authors.initials: A
authors.lastname: Shiotani
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kusunoki
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Kimura
authors.forename: Mnabu
authors.initials: M
authors.lastname: Ishii
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Imamura
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Tarumi
authors.forename: Noriaki
authors.initials: N
authors.lastname: Manabe
authors.forename: Tomoari
authors.initials: T
authors.lastname: Kamada
authors.forename: Jiro
authors.initials: J
authors.lastname: Hata
authors.forename: Ken
authors.initials: K
authors.lastname: Haruma
mesh_terms: D000328:Adult; D044466:Asian People; D001706:Biopsy; D016022:Case-Control Studies; D003093:Colitis, Ulcerative / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism*; D043183:Irritable Bowel Syndrome / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012007:Rectum / Q000378:metabolism / Q000473:pathology; D009418:S100 Proteins / Q000235:genetics / Q000378:metabolism*; D050486:Serotonin Plasma Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D005819:Genetic Markers; D012333:RNA, Messenger; D009418:S100 Proteins; C110294:S100A1 protein; D050486:Serotonin Plasma Membrane Transport Proteins; D016753:Interleukin-10