title: Conjugation of NaGdF4 upconverting nanoparticles on silica nanospheres as contrast agents for multi-modality imaging.
abstract: Here, we report the covalently conjugation of lanthanide doped NaGdF4:Yb(3+), Er(3+)@NaGdF4 upconverting nanoparticles (UCNPs) on methylphosphonate functionalized silica nanospheres (pSi NPs) for in vivo upconversion luminescence (UCL), T1-weighted magnetic resonance (MR), and X-ray computed tomography (CT) multi-modality imaging. The nanocomposites (pSi@UCNPs) were synthesized by a facile ligand exchange strategy. The hydrophobic pSi@UCNPs were transferred into aqueous solution by surface coating Pluronic F127. The Pluronic F127 coated pSi@UCNPs (pSi@UCNPs@F127) exhibit excellent stability in biological medium, inappreciable cytotoxicity and negligible organ toxicity. The pSi@UCNPs@F127 also shows brighter UCL, and higher CT/MR enhancements than that of Pluronic F127 coated NaGdF4:Yb(3+), Er(3+)@NaGdF4 UCNP. In detail, the capability of pSi@UCNPs@F127 as high performance contrast agents for in vivo multi-modality (UCL/MR/CT) imaging is evaluated successfully through small-animal experiments.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China.
authors.forename: Fuyao
authors.initials: F
authors.lastname: Liu
authors.forename: Xiuxia
authors.initials: X
authors.lastname: He
authors.forename: Liang
authors.initials: L
authors.lastname: Liu
authors.forename: Hongpeng
authors.initials: H
authors.lastname: You
authors.forename: Huimao
authors.initials: H
authors.lastname: Zhang
authors.forename: Zhenxin
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D016923:Cell Death; D045744:Cell Line, Tumor; D003287:Contrast Media; D003952:Diagnostic Imaging / Q000379:methods*; D005459:Fluorides; D005682:Gadolinium; D006801:Humans; D008279:Magnetic Resonance Imaging; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D054118:Nanospheres / Q000648:ultrastructure; D009369:Neoplasms / Q000175:diagnosis / Q000473:pathology; D020442:Poloxamer / Q000737:chemistry; D012822:Silicon Dioxide; D013057:Spectrum Analysis; D014057:Tomography, X-Ray Computed
chemical_list: D003287:Contrast Media; D020442:Poloxamer; D012822:Silicon Dioxide; D005682:Gadolinium; D005459:Fluorides