title: Rational medium optimization based on comparative metabolic profiling analysis to improve fumaric acid production.
abstract: To rationally guide fumaric acid production improvement, metabolic profiling approach was performed to analyze metabolite changes of Rhizopus oryzae FM19 under different fermentation conditions. A correlation between the metabolic profiling and fumaric acid production was revealed by principal component analysis as well as partial least squares. Citric acid, oxaloacetic acid, 2-oxoglutarate, lactic acid, proline, alanine, valine, leucine were identified to be mainly responsible for the metabolism difference, which were involved in the Embden-Meyerhof-Parnas, tricarboxylic acid cycle, amino acid metabolism and fatty acid metabolism. Through the further analysis of metabolites changes together with the above pathways, exogenous addition strategies were developed, which resulted in 14% increase of fumaric acid (up to 56.5 g/L) and less by-products. These results demonstrated that metabolic profiling analysis could be successfully applied to the rational guidance of medium optimization and the productivity improvement of value-added compounds.
authors.affiliation: Department of Biological Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors.forename: Guanyi
authors.initials: G
authors.lastname: Wang
authors.forename: Di
authors.initials: D
authors.lastname: Huang
authors.forename: Haishan
authors.initials: H
authors.lastname: Qi
authors.forename: Jianping
authors.initials: J
authors.lastname: Wen
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Jia
authors.forename: Yunlin
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D002244:Carbon / Q000378:metabolism; D003470:Culture Media; D005227:Fatty Acids / Q000378:metabolism; D005285:Fermentation; D005650:Fumarates / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D006863:Hydrogen-Ion Concentration; D016018:Least-Squares Analysis; D055442:Metabolome; D055432:Metabolomics; D025341:Principal Component Analysis; D012233:Rhizopus / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D003470:Culture Media; D005227:Fatty Acids; D005650:Fumarates; D002244:Carbon; C032005:fumaric acid