title: Distribution and expression of Kirre, an IgSF molecule, during postnatal development of rat cerebellum.
abstract: Immunoglobulin superfamily (IgSF) molecules are actively involved in cell-cell adhesion, neuronal migration, axonal guidance and synapse formation in the nervous system. Kirre, as a member of this family, has been implicated in mammalian neuronal differentiation and development. Although the distribution of rKirre (a rat homologue of Drosophila Kirre) mRNA was previously analyzed in adult rat cerebellum by in situ hybridization, the expression levels of transcript and protein were not well studied. Here, we showed that the expressions of rKirre mRNA and protein significantly increased during postnatal development of rat cerebellum. rKirre mRNA was mainly expressed in the granular layers and Purkinje cell layer in the developing cerebellum, revealing a possible involvement of rKirre in granule cell migration and Purkinje cell development. An essential relationship between rKirre and Purkinje cells was implied by the co-localization of rKirre and NF-200 on the cell bodies of Purkinje cells. These results suggest that rKirre may play a potential role in postnatal developing rat cerebellum.
authors.affiliation: Department of Surgery, Mercer University School of Medicine and Medical Center of Central Georgia, Macon, GA, USA.
authors.forename: Lingwei
authors.initials: L
authors.lastname: Kong
authors.forename: Roy C Y
authors.initials: RC
authors.lastname: Choi
authors.forename: Karl W K
authors.initials: KW
authors.lastname: Tsim
authors.forename: Naihe
authors.initials: N
authors.lastname: Jing
authors.forename: Don K
authors.initials: DK
authors.lastname: Nakayama
authors.forename: Zhongbiao
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D002465:Cell Movement; D002531:Cerebellum / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D016900:Neurofilament Proteins / Q000378:metabolism; D011689:Purkinje Cells / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C486607:Kirrel1 protein, rat; D008565:Membrane Proteins; D016900:Neurofilament Proteins; C051774:neurofilament protein H