title: Molecular detection of Theileria species in sheep from northern China.
abstract: Ovine theileriosis is a tick-borne disease that restricts the development of small ruminant husbandry in northern China. In this study, we report on a molecular epidemiological survey of ovine Theileria spp. in 198 blood samples taken from sheep in northern China. The DNA samples were screened by a nested polymerase chain reaction (PCR) targeting the 18S rRNA gene of ovine Theileria spp. The prevalence of ovine Theileria spp. in Yanji, Nongan, Longjing, Toudao and Jinchang was 80%, 40%, 37%, 24% and 32%, respectively. The sequencing analyses approved the present of the T. orientalis and/or T. luwenshuni in these regions. Taken together, we have demonstrated a high incidence of Theileria spp. in northern China that calls for the need to design effective control programs for ovine theileriosis.
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Shinuo
authors.initials: S
authors.lastname: Cao
authors.forename: Shoufa
authors.initials: S
authors.lastname: Zhang
authors.forename: Lijun
authors.initials: L
authors.lastname: Jia
authors.forename: Shujiang
authors.initials: S
authors.lastname: Xue
authors.forename: Longzheng
authors.initials: L
authors.lastname: Yu
authors.forename: Ketsarin
authors.initials: K
authors.lastname: Kamyingkird
authors.forename: Paul Franck Adjou
authors.initials: PF
authors.lastname: Moumouni
authors.forename: Ahmed Abd El Moniem
authors.initials: AA
authors.lastname: Moussa
authors.forename: Mo
authors.initials: M
authors.lastname: Zhou
authors.forename: Yuanming
authors.initials: Y
authors.lastname: Zhang
authors.forename: Mohamad Alaa
authors.initials: MA
authors.lastname: Terkawi
authors.forename: Tatsunori
authors.initials: T
authors.lastname: Masatani
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Nishikawa
authors.forename: Xuenan
authors.initials: X
authors.lastname: Xuan
mesh_terms: D000818:Animals; D001483:Base Sequence; D002681:China / Q000453:epidemiology; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D005843:Geography; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D015995:Prevalence; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012756:Sheep; D012757:Sheep Diseases / Q000453:epidemiology* / Q000469:parasitology*; D016794:Theileria / Q000235:genetics*; D013801:Theileriasis / Q000453:epidemiology*
chemical_list: D017931:DNA Primers; D012337:RNA, Ribosomal, 18S