title: Electrostatic and hydrophobic interactions differentially tune membrane binding kinetics of the C2 domain of protein kinase Cα.
abstract: The cellular activation of conventional protein kinase C (PKC) isozymes is initiated by the binding of their C2 domains to membranes in response to elevations in intracellular Ca(2+). Following this C2 domain-mediated membrane recruitment, the C1 domain binds its membrane-embedded ligand diacylglycerol, resulting in activation of PKC. Here we explore the molecular mechanisms by which the C2 domain controls the initial step in the activation of PKC. Using stopped-flow fluorescence spectroscopy to measure association and dissociation rate constants, we show that hydrophobic interactions are the major driving force in the binding of the C2 domain to anionic membranes, whereas electrostatic interactions dominate in membrane retention. Specifically, mutation of select hydrophobic or select basic residues in the Ca(2+)-binding loops reduces membrane affinity by distinct mechanisms; mutation of hydrophobic residues primarily alters association rate constants, whereas mutation of charged residues affects dissociation rate constants. Live cell imaging reveals that introduction of these mutations into full-length PKCα not only reduces the Ca(2+)-dependent translocation to plasma membrane but, by impairing the plasma membrane-sensing role of the C2 domain, causes phorbol ester-triggered redistribution of PKCα to other membranes, such as the Golgi. These data underscore the key role of the C2 domain in driving conventional PKC isozymes to the plasma membrane and reveal that not only the amplitude but also the subcellular location of conventional PKC signaling can be tuned by altering the affinity of this module for membranes.
authors.affiliation: Department of Pharmacology, La Jolla, California 92093-0721; Biomedical Sciences Graduate Program, University of California San Diego, La Jolla, California 92093-0721.
authors.forename: Angela M
authors.initials: AM
authors.lastname: Scott
authors.affiliation: Department of Pharmacology, La Jolla, California 92093-0721; Biomedical Sciences Graduate Program, University of California San Diego, La Jolla, California 92093-0721.
authors.forename: Corina E
authors.initials: CE
authors.lastname: Antal
authors.affiliation: Department of Pharmacology, La Jolla, California 92093-0721. Electronic address: anewton@ucsd.edu.
authors.forename: Alexandra C
authors.initials: AC
authors.lastname: Newton
mesh_terms: D000818:Animals; D019556:COS Cells; D002118:Calcium / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology* / Q000235:genetics; D002522:Chlorocebus aethiops; D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D057927:Hydrophobic and Hydrophilic Interactions; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D007700:Kinetics; D009154:Mutation; D051571:Protein Kinase C-alpha / Q000235:genetics / Q000378:metabolism*; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology; D055672:Static Electricity
chemical_list: D007527:Isoenzymes; C496628:PRKCA protein, human; D051571:Protein Kinase C-alpha; D002118:Calcium
keywords: C2 Domain; Calcium; Calcium Signaling; Calcium-binding Proteins; Membrane Translocation; Protein Kinase C (PKC); Signal Transduction