title: Investigation of SSEA-4 binding protein in breast cancer cells.
abstract: SSEA-4, a sialyl-glycolipid, has been commonly used as a pluripotent human embryonic stem cell marker, and its expression is correlated with the metastasis of some malignant tumors. However, there is no in-depth functional study related to the receptor and the role of this glycolipid. Here, we report the identification of an SSEA-4-binding protein in a breast cancer cell line, MCF-7. By using affinity capture and glycan microarray techniques, the intracellular FK-506 binding protein 4 (FKBP4) was identified to bind directly to SSEA-4. The biological significance of SSEA-4/FKBP4 interaction was investigated.
authors.affiliation: Department of Chemistry, National Taiwan University, Taipei 106, Taiwan.
authors.forename: Ting-Chun
authors.initials: TC
authors.lastname: Hung
authors.forename: Chih-Wei
authors.initials: CW
authors.lastname: Lin
authors.forename: Tsui-Ling
authors.initials: TL
authors.lastname: Hsu
authors.forename: Chung-Yi
authors.initials: CY
authors.lastname: Wu
authors.forename: Chi-Huey
authors.initials: CH
authors.lastname: Wong
mesh_terms: D001943:Breast Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D013058:Mass Spectrometry; D046228:Microarray Analysis; D011092:Polyethylene Glycols / Q000737:chemistry; D011134:Polysaccharides / Q000378:metabolism; D011485:Protein Binding; D055746:Stage-Specific Embryonic Antigens / Q000378:metabolism*; D016559:Tacrolimus / Q000378:metabolism; D022021:Tacrolimus Binding Proteins / Q000378:metabolism
chemical_list: D011134:Polysaccharides; D055746:Stage-Specific Embryonic Antigens; C066584:stage-specific embryonic antigen-4; D011092:Polyethylene Glycols; D022021:Tacrolimus Binding Proteins; D016559:Tacrolimus