title: Antiepileptic activity of lobeline isolated from the leaf of Lobelia nicotianaefolia and its effect on brain GABA level in mice.
abstract: OBJECTIVE
  To investigate the anticonvulsant activity of the lobeline isolated from the Lobelia nicotianaefolia in chemoconvulsant-induced seizures and its biochemical mechanism by investigating relationship between seizure activities and altered gamma amino butyric acid (GABA) in brain of mice in Pentylenetetrazol (PTZ) seizure models.
  
  
  METHODS
  The anticonvulsant activity of the isolated lobeline (5, 10, 20 and 30 mg/kg, i.p.) was investigated in PTZ and strychnine induced seizures in mice and the effect of isolated lobeline on brain GABA level in seizures induced by PTZ. Diazepam was used as reference anticonvulsant drugs for comparison.
  
  
  RESULTS
  Isolated lobeline (10, 20 and 30 mg/kg, i.p.) significantly delayed and antagonized (P < 0.050-0.001) the onset of PTZ-induced seizures. It also antagonized strychnine induced seizures. The mortality was also prevented in the test group of animals. In biochemical evaluation, isolated lobeline (5, 10 and 20 mg/kg, i.p.) significantly increased the brain GABA level. And at dose of 30 mg/kg GABA level showed slight decrease in PTZ model.
  
  
  CONCLUSIONS
  In our findings, isolated lobeline (20mg/kg) exhibited potent anticonvulsant activity against PTZ induced seizures. Also a biochemical evaluation suggested significant increase in barain GABA level at 20 mg/kg i.p. of isolated lobeline. Hence, we may propose that lobeline reduces epileptic seizures by enhancing the GABA release supporting the GABAergic mechanism.
authors.affiliation: Department of Pharmacognosy, Allana College of Pharmacy, University of Pune, Pune, Maharashtra, 411001 India.
authors.forename: Abrar M
authors.initials: AM
authors.lastname: Tamboli
authors.forename: Rukhsana A
authors.initials: RA
authors.lastname: Rub
authors.forename: Pinaki
authors.initials: P
authors.lastname: Ghosh
authors.forename: S L
authors.initials: SL
authors.lastname: Bodhankar
mesh_terms: D000818:Animals; D000927:Anticonvulsants / Q000008:administration & dosage* / Q000302:isolation & purification; D001921:Brain / Q000187:drug effects*; D001923:Brain Chemistry; D003292:Convulsants / Q000008:administration & dosage; D004195:Disease Models, Animal; D029746:Lobelia / Q000737:chemistry*; D008120:Lobeline / Q000008:administration & dosage* / Q000302:isolation & purification; D008297:Male; D051379:Mice; D010433:Pentylenetetrazole / Q000008:administration & dosage; D010936:Plant Extracts / Q000008:administration & dosage / Q000302:isolation & purification; D012640:Seizures / Q000139:chemically induced / Q000188:drug therapy*; D005680:gamma-Aminobutyric Acid / Q000032:analysis*
chemical_list: D000927:Anticonvulsants; D003292:Convulsants; D010936:Plant Extracts; D005680:gamma-Aminobutyric Acid; D008120:Lobeline; D010433:Pentylenetetrazole
keywords: Antiepileptic activity; Brain GABA level; Lobelia nicotianaefolia; Lobeline; Pentylenetetrazol (PTZ); Strychnine