title: A novel approach to automated genotyping of Mycobacterium tuberculosis using a panel of 15 MIRU VNTRs.
abstract: In this study, we present a new approach to variable number tandem repeats (VNTR) analysis using the QIAxcel capillary electrophoresis system and a software-integrated peak calling function. Allelic ladders representing 15 mycobacterial interspersed repetitive units (MIRU)-VNTR loci were used to define peak calling tables thereby enabling high precision Mycobacterium tuberculosis strain identification.
authors.affiliation: Osaka Prefectural Medical Center for Respiratory and Allergic Diseases, Japan. tom_matsumoto@sutv.zaq.ne.jp
authors.forename: Tomoshige
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Koshii
authors.forename: Kazu
authors.initials: K
authors.lastname: Sakane
authors.forename: Tomomi
authors.initials: T
authors.lastname: Murakawa
authors.forename: Yukio
authors.initials: Y
authors.lastname: Hirayama
authors.forename: Hisako
authors.initials: H
authors.lastname: Yoshida
authors.forename: Masashi
authors.initials: M
authors.lastname: Kurokawa
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Tamura
authors.forename: Takayuki
authors.initials: T
authors.lastname: Nagai
authors.forename: Ichiro
authors.initials: I
authors.lastname: Kawase
mesh_terms: D057205:Automation, Laboratory / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics*; D019075:Electrophoresis, Capillary / Q000379:methods; D018598:Minisatellite Repeats; D058889:Molecular Typing / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial