title: An in vivo tagging method reveals that Ras undergoes sustained activation upon transglutaminase-mediated protein serotonylation.
abstract: Don't interrupt! Protein serotonylation has been implicated in living cells, yet its role remains poorly defined because of the lack of characterization tools. We synthesized a serotonin derivative to enable selective tagging of serotonylation and to investigate its effect on Ras; the latter displayed undisrupted interaction with Raf-1 at the Ras binding domain.
authors.affiliation: Institute of Biological Chemistry, Academia Sinica, 128 Academia Road Section 2, Taipei 11529, Taiwan.
authors.forename: Jason Ching-Yao
authors.initials: JC
authors.lastname: Lin
authors.forename: Chi-Chi
authors.initials: CC
authors.lastname: Chou
authors.forename: Shijay
authors.initials: S
authors.lastname: Gao
authors.forename: Shang-Chuen
authors.initials: SC
authors.lastname: Wu
authors.forename: Kay-Hooi
authors.initials: KH
authors.lastname: Khoo
authors.forename: Chun-Hung
authors.initials: CH
authors.lastname: Lin
mesh_terms: D045744:Cell Line, Tumor; D004789:Enzyme Activation; D006801:Humans; D011499:Protein Processing, Post-Translational; D012701:Serotonin / Q000031:analogs & derivatives / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D011503:Transglutaminases / Q000378:metabolism*; D018631:ras Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D012701:Serotonin; D011503:Transglutaminases; D018631:ras Proteins