title: Label-free cell profiling.
abstract: A surface plasmon resonance (SPR) array imaging method is outlined for label-free cell profiling. Red blood cells (RBCs) were injected into a flow chamber on top of a spotted sensor surface. Spots contained antibodies to various RBC membrane antigens. A typical sensorgram showed an initial response corresponding to cell sedimentation (S) followed by a specific upward response (T) corresponding to specific binding of cells during a critical wash step. The full analysis cycle for RBC profiling was less than 6 min. The sensor surface could be regenerated at least 100 times, allowing the determination of a cell surface antigen profile of RBCs.
authors.affiliation: Medical Cell Biophysics Group, MIRA Institute, University of Twente, Enschede, The Netherlands. r.b.m.schasfoort@utwente.nl
authors.forename: Richard B M
authors.initials: RB
authors.lastname: Schasfoort
authors.forename: Arthur E H
authors.initials: AE
authors.lastname: Bentlage
authors.forename: Ivan
authors.initials: I
authors.lastname: Stojanovic
authors.forename: Alex
authors.initials: A
authors.lastname: van der Kooi
authors.forename: Ellen
authors.initials: E
authors.lastname: van der Schoot
authors.forename: Leon W M M
authors.initials: LW
authors.lastname: Terstappen
authors.forename: Gestur
authors.initials: G
authors.lastname: Vidarsson
mesh_terms: D000906:Antibodies / Q000276:immunology; D000941:Antigens / Q000276:immunology; D004912:Erythrocytes / Q000166:cytology* / Q000276:immunology; D006801:Humans; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: D000906:Antibodies; D000941:Antigens