title: Effect of linoleic acids on the release of β-carotene from carotenoid-producing Saccharomyces cerevisiae into sunflower oil.
abstract: In situ extraction is important for highly productive and cost-efficient processes in industrial biotechnology, but it is difficult to establish for intracellularly accumulating carotenoids like β-carotene. In this study, the organic solvent used in aqueous-organic two-phase media exerted a strong effect on the release of β-carotene from recombinant yeast cells. The carotenoid-synthesizing Saccharomyces cerevisiae strain YB/I/E was cultivated in two-liquid-phase media with 20% dodecane or 20% sunflower oil. Up to 0.6 µg/ml β-carotene was released into sunflower oil, but less than 0.1 µg/ml into dodecane, although biocompatibility and solubility of β-carotene is appropriate for both solvents. Addition of linoleic acid, the main component of sunflower oil, to the dodecane phase increased the amount of β-carotene released, indicating that linoleic acid is the component responsible for the β-carotene release into sunflower oil. These findings demonstrate that the effect of the organic solvent should be taken into consideration for further research on in situ extraction of carotenoids.
authors.affiliation: DECHEMA Research Institute, Frankfurt am Main, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Sonntag
authors.forename: Isabell
authors.initials: I
authors.lastname: Schmidt
authors.forename: Markus
authors.initials: M
authors.lastname: Buchhaupt
authors.forename: Jens
authors.initials: J
authors.lastname: Schrader
mesh_terms: D002338:Carotenoids / Q000378:metabolism*; D008041:Linoleic Acids / Q000494:pharmacology*; D010938:Plant Oils; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000378:metabolism*; D000074242:Sunflower Oil; D019207:beta Carotene / Q000378:metabolism*
chemical_list: D008041:Linoleic Acids; D010938:Plant Oils; D000074242:Sunflower Oil; D019207:beta Carotene; D002338:Carotenoids