title: Nox2 deficiency prevents hypertension-induced vascular dysfunction and hypertrophy in cerebral arterioles.
abstract: Oxidative stress is involved in many hypertension-related vascular diseases in the brain, including stroke and dementia. Thus, we examined the role of genetic deficiency of NADPH oxidase subunit Nox2 in the function and structure of cerebral arterioles during hypertension. Arterial pressure was increased in right-sided cerebral arterioles with transverse aortic banding for 4 weeks in 8-week-old wild-type (WT) and Nox2-deficient (-/y) mice. Mice were given N(G)-nitro-L-arginine methyl ester (L-NAME, 10 mg/kg) or vehicle to drink. We measured the reactivity in cerebral arterioles through open cranial window in anesthetized mice and wall cross-sectional area and superoxide levels ex vivo. Aortic constriction increased systolic and pulse pressures in right-sided carotid arteries in all groups of mice. Ethidium fluorescence showed increased superoxide in right-sided cerebral arterioles in WT, but not in Nox2-/y mice. Dilation to acetylcholine, but not sodium nitroprusside, was reduced, and cross-sectional areas were increased in the right-sided arterioles in WT, but were unchanged in Nox2-/y mice. L-NAME reduced dilation to acetylcholine but did not result in hypertrophy in right-sided arterioles of Nox2-/y  mice. In conclusion, hypertension-induced superoxide production derived from Nox2-containing NADPH oxidase promotes hypertrophy and causes endothelial dysfunction in cerebral arterioles, possibly involving interaction with nitric oxide.
authors.affiliation: Department of Pathology, University of Iowa Carver College of Medicine, 5231D RCP, 200 Hawkins Drive, Iowa City, IA 52242, USA ; Department of Neurological Sciences, University of Vermont, 149 Beaumont Avenue, HSRF 416, Burlington, VT 05405, USA.
authors.forename: Siu-Lung
authors.initials: SL
authors.lastname: Chan
authors.forename: Gary L
authors.initials: GL
authors.lastname: Baumbach