title: [Fast screening ninety-six pesticides in six kinds of agricultural products by high performance liquid chromatography-quadrupole/electrostatic field orbit trap high-resolution mass spectrometry].
abstract: A high-throughput method for the determination of 96 pesticides in six kinds of agricultural products by liquid chromatography-quadrupole/electrostatic field orbit trap high-resolution mass spectrometry was developed. After extraction with 0.1% acetic acid in acetonitrile solution and concentration, dispersive solid-phase extraction was further utilized to reduce the matrix interference. The chromatographic analysis was performed on a C18 column with methanol and 5 mmol/L ammonium acetate solution as the mobile phases with a gradient elution program. The 96 pesticide residues were analyzed in switching positive and negative modes at the same time. With the optimized mass resolution, accurate mass-to-charge ratio extraction of the target pesticide compounds in full scan mode could eliminate matrix interference effectively. Two-stage threshold-triggered full mass scan mode was utilized to further improve the accuracy of qualitative analysis. The linear ranges of all the 96 pesticides were from 1 microg/L to 200 microg/L with correlation coefficients greater than 0.99. By detecting spiked samples, the detection limits were 5 microg/kg for all the residues and the recoveries were in the range of 58% - 105% with the relative standard deviations (RSDs) between 8.8% and 18.3%.
authors.affiliation: Animal, Plant and Food Inspection Center of Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing 20001, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
authors.forename: Tao
authors.initials: T
authors.lastname: Ding
authors.forename: Han
authors.initials: H
authors.lastname: Liu
authors.forename: Huilan
authors.initials: H
authors.lastname: Chen
authors.forename: Zengyun
authors.initials: Z
authors.lastname: Zhao
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.forename: Chongyu
authors.initials: C
authors.lastname: Shen
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002523:Edible Grain / Q000737:chemistry; D005506:Food Contamination / Q000032:analysis; D013058:Mass Spectrometry; D010573:Pesticide Residues / Q000032:analysis*; D011198:Solanum tuberosum / Q000737:chemistry*; D013025:Glycine max / Q000737:chemistry*; D014675:Vegetables / Q000737:chemistry
chemical_list: D010573:Pesticide Residues