title: [Mutation of lambdapL/pR-cI857 system for production of bacterial ghost in Escherichia coli].
abstract: Bacterial ghost is intact envelope of Gram-negative bacteria, which is produced by the function of the lysis gene E from bacteriophage PhiX174. The expression of the lysis gene E is usually controlled by the thermosensitive lambdapL/pR-cI857 promoter. In this study, we described a mutation (T --> C) at the ninth nucleotide of the OR2 in the lambdapR promoter of the lambdapL/pR-cI857 system by overlap PCR. The bacteriolytic assay showed that the mutation in the lambdapL/pR-cI857 system enhanced the temperature of repressing the expression of gene E up to 37 degrees C. The lysis efficiency of altered lambdapR promoter in Escherichia coli DH5a and avian pathogenic E. coli DE17 was up to 99.9%. The expanded range of temperature will benefit for the production of bacterial ghost.
authors.affiliation: School of Animal Science and Technology, Shandong Agricultural University, Taian 271100, Shandong, China.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Dong
authors.forename: Xian'gan
authors.initials: X
authors.lastname: Han
authors.forename: Hao
authors.initials: H
authors.lastname: Bai
authors.forename: Liang
authors.initials: L
authors.lastname: He
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.forename: Tongjie
authors.initials: T
authors.lastname: Chai
authors.forename: Chan
authors.initials: C
authors.lastname: Ding
authors.forename: Haiwen
authors.initials: H
authors.lastname: Liu
authors.forename: Shengqing
authors.initials: S
authors.lastname: Yu
mesh_terms: D001433:Bacteriolysis / Q000502:physiology*; D010582:Bacteriophage lambda / Q000235:genetics*; D001483:Base Sequence; D002462:Cell Membrane / Q000502:physiology; D004269:DNA, Bacterial / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000502:physiology* / Q000821:virology; D005786:Gene Expression Regulation / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D011401:Promoter Regions, Genetic / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; C029555:E protein, bacteriophage X174; D014764:Viral Proteins