title: Modeling and analysis of Schistosoma Argonaute protein molecular spatial conformation.
abstract: OBJECTIVE
  To analyze the amino acid sequence composition, secondary structure, the spatial conformation of its domain and other characteristics of Argonaute protein.
  
  
  METHODS
  Bioinformatics tools and the internet server were used. Firstly, the amino acid sequence composition features of the Argonaute protein were analyzed, and the phylogenetic tree was constructed. Secondly, Argonaute protein's distribution of secondary structure and its physicochemical properties were predicted. Lastly, the protein functional expression form of the domain group was established through the Phyre-based analysis on the spatial conformation of Argonaute protein domains.
  
  
  RESULTS
  593 amino acids were encoded by Argonaute protein, the phylogenetic tree was constructed, and Argonaute protein's distribution of secondary structure and its physicochemical properties were obtained through analysis. In addition, the functional expression form which comprised the N-terminal PAZ domain and C-terminal Piwi domain for the Argonaute protein was obtained with Phyre.
  
  
  CONCLUSIONS
  The information relationship between the structure and function of the Argonaute protein can be initially established with bioinformatics tools and the internet server, and this provides the theoretical basis for further clarifying the function of Schistosoma Argonaute protein.
authors.affiliation: The Faculty of Biomedical Engineering of Zhengzhou University, Zhengzhou 450001, Henan Province, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhang
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Shang
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zhang
authors.forename: Yuntao
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D060565:Argonaute Proteins / Q000737:chemistry* / Q000235:genetics*; D055598:Chemical Phenomena; D016000:Cluster Analysis; D019295:Computational Biology / Q000379:methods; D008958:Models, Molecular; D010802:Phylogeny; D011487:Protein Conformation; D012547:Schistosoma / Q000737:chemistry* / Q000235:genetics*; D017386:Sequence Homology, Amino Acid
chemical_list: D060565:Argonaute Proteins
keywords: Amino acid sequence; Argonaute protein; Distribution of secondary structure; Domain; Phyre; Schistosoma; Spatial conformation