title: Studies on the effect of adenosine on calcium oscillation in hippocampal neurons.
abstract: Adenosine (Ade) is an antiepileptic agent. In order to investigate the possible mechanism of action of Ade, its effect on calcium (Ca2+) oscillations in hippocampal neurons of Sprague Dawley (SD) rats was explored. Primary hippocampal neurons were cultured from suckling neonatal SD rats. Cells were cultured for 7-9 days and the Ca2+oscillations in response to perfusion with Ade were detected using confocal laser scanning microscopy in combination with Fluo-3/AM labeling. This study found that Ade inhibits the spontaneous synchronized Ca2+oscillation frequency and amplitude in mature hippocampal neurons and such inhibition depends on the Ade dosage level to a certain extent. Ade also had a significant inhibitory effect on high potassium-induced Ca2+oscillation frequency and amplitude. Ade had a significant inhibitory effect on high-voltage-activated Ca2+channel-mediated Ca2+influx and Ca2+oscillations in neurons. This may be one of the mechanisms for Ade to exert antiepileptic effects as an endogenous substance.
authors.affiliation: Department of Neurology, The Affiliated Hospital of Binzhou Medical University, Binzhou 256603, P.R. China.
authors.forename: Jinbo
authors.initials: J
authors.lastname: Chen
authors.forename: Yuan
authors.initials: Y
authors.lastname: Wang
authors.forename: Yuliang
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiangming
authors.initials: X
authors.lastname: Yi
authors.forename: Ruli
authors.initials: R
authors.lastname: Ge
keywords: adenosine; calcium oscillation; confocal laser scanning microscope