title: Cratylia mollis 1, 4 lectin: a new biotechnological tool in IL-6, IL-17A, IL-22, and IL-23 induction and generation of immunological memory.
abstract: Cratylia mollis lectin has already established cytokine induction in Th1 and Th2 pathways. Thereby, this study aimed to evaluate Cramoll 1, 4 in IL-6, IL-17A, IL-22, and IL-23 induction as well as analyze immunologic memory mechanism by reinducing lymphocyte stimulation. Initially we performed a screening in cultured splenocytes where Cramoll 1, 4 stimulated IL-6 production 5x more than ConA (P < 0.05). The same behavior was observed with IL-22 where the increase was greater than 4x. Nevertheless, IL-17A induction was similar for both lectins. In PBMCs, the same splenocytes course was observed for IL-6 and IL-17A. Concerning the stimulation of IL-22 and IL-23 Cramoll 1, 4 was more efficient than ConA in cytokines stimulation mainly in IL-23 (P < 0.01). Analyzing reinduced lymphocyte stimulation, IL-17A production was higher (P < 0.001) when the first stimulus was realized with Cramoll 1, 4 at 1 μ g/mL and the second at 5 μ g/mL. IL-22 shows significant differences (P < 0.01) at the same condition. Nevertheless, IL-23 revels the best response when the first stimuli was realized with Cramoll1, 4 at 100 ng/mL and the second with 5 μ g/mL. We conclude that the Cramoll 1, 4 is able to induce IL-6, IL-17A, IL-22, and IL-23 cytokines in vitro better than Concavalin A, besides immunologic memory generation, being a potential biotechnological tool in Th17 pathway studies.
authors.affiliation: Laboratório de Imunomodulação e Novas Abordagens Terapêuticas, Universidade Federal de Pernambuco, Avenida Prof. Moraes Rêgo 1235, Cidade Universitária, 50670-901 Recife, PE, Brazil.
authors.forename: Priscilla Stela Santana
authors.initials: PS
authors.lastname: de Oliveira
authors.forename: Moacyr Jesus Barreto de Melo
authors.initials: MJ
authors.lastname: Rêgo
authors.forename: Rafael Ramos
authors.initials: RR
authors.lastname: da Silva
authors.forename: Mariana Brayner
authors.initials: MB
authors.lastname: Cavalcanti
authors.forename: Suely Lins
authors.initials: SL
authors.lastname: Galdino
authors.forename: Maria Tereza dos Santos
authors.initials: MT
authors.lastname: Correia
authors.forename: Luana Cassandra Breitenbach Barroso
authors.initials: LC
authors.lastname: Coelho
authors.forename: Maira Galdino da Rocha
authors.initials: MG
authors.lastname: Pitta
mesh_terms: D003208:Concanavalin A / Q000008:administration & dosage / Q000276:immunology; D007887:Fabaceae; D020381:Interleukin-17 / Q000276:immunology / Q000378:metabolism; D053759:Interleukin-23 / Q000276:immunology / Q000378:metabolism; D015850:Interleukin-6 / Q000276:immunology / Q000378:metabolism; D007378:Interleukins / Q000276:immunology / Q000378:metabolism; D037102:Lectins / Q000008:administration & dosage* / Q000276:immunology; D008213:Lymphocyte Activation / Q000187:drug effects* / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D000097043:Interleukin-22
chemical_list: D003208:Concanavalin A; D020381:Interleukin-17; D053759:Interleukin-23; D015850:Interleukin-6; D007378:Interleukins; D037102:Lectins; D000097043:Interleukin-22