title: A click-and-release pyrrolysine analogue.
abstract: What's the catch? A pyrrolysine analogue bearing a terminal alkyne and an ester functionality can be incorporated into recombinant proteins and render them amenable to capture by the click reaction and subsequent release through ester hydrolysis. The utility of this pyrrolysine-inspired technology is demonstrated for the identification of SUMOylation sites.
authors.affiliation: School of Life Sciences, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China.
authors.forename: Marianne M
authors.initials: MM
authors.lastname: Lee
authors.forename: Tomasz
authors.initials: T
authors.lastname: Fekner
authors.forename: Tsz-Ho
authors.initials: TH
authors.lastname: Tang
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Aurora Ho-Yin
authors.initials: AH
authors.lastname: Chan
authors.forename: Pang-Hung
authors.initials: PH
authors.lastname: Hsu
authors.forename: Shannon W
authors.initials: SW
authors.lastname: Au
authors.forename: Michael K
authors.initials: MK
authors.lastname: Chan
mesh_terms: D057930:Click Chemistry; D006801:Humans; D008239:Lysine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D058207:Sumoylation; D044502:Thymine DNA Glycosylase / Q000737:chemistry / Q000378:metabolism
chemical_list: D044502:Thymine DNA Glycosylase; C456839:pyrrolysine; D008239:Lysine