title: Identification of β-amyloid species in canine cerebrospinal fluid by mass spectrometry.
abstract: It is well known that several Aβ species, including Aβ40 and Aβ42, are present in cerebrospinal fluid (CSF). Experimental results suggest that these species could play a role in Alzheimer's disease and might also have diagnostic significance. In the present work, the canine CSF β-amyloid species profile has been identified by matrix-assisted laser desorption and ionization-time-of-flight/time of flight mass spectrometry (MALDI-TOF/TOF) analysis after immunoprecipitation with different Aβ-specific antibodies. The results show that species arising from combined β- and γ-secretase activities in humans, such as Aβ1-33, Aβ1-34, Aβ1-37, Aβ1-38, Aβ1-39, Aβ1-40, and Aβ1-42, are also present in dogs. Species arising from combined α- and β-secretase activities, as well as other Aβ-degrading enzymes, are also present in both human and canine CSF, with the exception of Aβ1-13, Aβ1-14, and Aβ1-18, which are not detected in dogs. A large number of species truncated at Glu-3 and Glu-11 have also been detected. To our knowledge, this work describes a most complete Aβ species profile from canine CSF. The similarities between the canine and human CSF Aβ profile reinforce the dog as a highly appropriate animal model for research in Alzheimer's disease.
authors.affiliation: R&D Laboratory, Araclon Biotech Ltd, Zaragoza, Spain. msarasa@araclon.com
authors.forename: Leticia
authors.initials: L
authors.lastname: Sarasa
authors.forename: José Antonio
authors.initials: JA
authors.lastname: Allué
authors.forename: Pedro
authors.initials: P
authors.lastname: Pesini
authors.forename: Angela
authors.initials: A
authors.lastname: González-Martínez
authors.forename: Manuel
authors.initials: M
authors.lastname: Sarasa
mesh_terms: D000544:Alzheimer Disease / Q000175:diagnosis / Q000209:etiology; D053829:Amyloid Precursor Protein Secretases / Q000134:cerebrospinal fluid; D016229:Amyloid beta-Peptides / Q000134:cerebrospinal fluid*; D000818:Animals; D015415:Biomarkers / Q000134:cerebrospinal fluid; D004195:Disease Models, Animal; D004285:Dogs; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*
chemical_list: D016229:Amyloid beta-Peptides; D015415:Biomarkers; D053829:Amyloid Precursor Protein Secretases