title: Evidence of oxidative stress in young and aged DJ-1-deficient mice.
abstract: Loss of DJ-1 function contributes to pathogenesis in Parkinson's disease. Here, we investigate the impact of aging and DJ-1 deficiency in transgenic mice. Ventral midbrain from young DJ-1-deficient mice revealed no change in 4-hydroxy-2-nonenal (4-HNE), but HSP60, HSP40 and striatal dopamine turnover were significantly elevated compared to wildtype. In aged mice, the chaperone response observed in wildtype animals was absent from DJ-1-deficient transgenics, and nigral 4-HNE immunoreactivity was enhanced. These changes were concomitant with increased striatal dopamine levels and uptake. Thus, increased oxidants and diminished protein quality control may contribute to nigral oxidative damage with aging in the model.
authors.affiliation: The Parkinson's Institute, Department of Basic Research, Sunnyvale, CA, USA.
authors.forename: Arjun V
authors.initials: AV
authors.lastname: Raman
authors.forename: Vivian P
authors.initials: VP
authors.lastname: Chou
authors.forename: Jennifer
authors.initials: J
authors.lastname: Atienza-Duyanen
authors.forename: Donato A
authors.initials: DA
authors.lastname: Di Monte
authors.forename: Frederick P
authors.initials: FP
authors.lastname: Bellinger
authors.forename: Amy B
authors.initials: AB
authors.lastname: Manning-Boğ
mesh_terms: D000367:Age Factors; D000375:Aging / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D001923:Brain Chemistry / Q000235:genetics; D059290:Dopaminergic Neurons / Q000378:metabolism / Q000502:physiology; D005260:Female; D008297:Male; D008636:Mesencephalon / Q000378:metabolism / Q000502:physiology; D051379:Mice; D008822:Mice, Transgenic; D017072:Neostriatum / Q000378:metabolism; D015513:Oncogene Proteins / Q000235:genetics*; D018384:Oxidative Stress / Q000235:genetics*; D054464:Peroxiredoxins; D000071617:Protein Deglycase DJ-1; D009435:Synaptic Transmission / Q000235:genetics / Q000502:physiology
chemical_list: D015513:Oncogene Proteins; D054464:Peroxiredoxins; C503466:PARK7 protein, mouse; D000071617:Protein Deglycase DJ-1