title: Mucilaginibacter calamicampi sp. nov., a member of the family Sphingobacteriaceae isolated from soil at a field of reeds.
abstract: A Gram-negative, aerobic, non-flagellated, non-gliding, rod-shaped bacterial strain, designated WR-R1Y(T), was isolated from soil at a field of reeds in South Korea. Strain WR-R1Y(T) grew optimally at 30 °C, at pH 7.0-7.5 and in the absence of NaCl. Neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain WR-R1Y(T) fell within the clade comprising Mucilaginibacter species, coherently clustering with the type strain of Mucilaginibacter composti, with which it exhibited the highest 16S rRNA gene sequence similarity value of 97.6 %. Sequence similarities to the type strains of the other Mucilaginibacter species and the other species used in the phylogenetic analysis were 93.1-96.9 % and <91.1 %, respectively. Strain WR-R1Y(T) contained MK-7 as the predominant menaquinone and iso-C15:0, summed feature 3 (C16:1 ω6c and/or C16:1 ω7c), C16:0 and iso-C17:0 3-OH as the major fatty acids. The major polar lipids were phosphatidylethanolamine and one unidentified aminophospholipid. The DNA G+C content of strain WR-R1Y(T) was 43.1 mol% and its mean DNA-DNA relatedness value with M. composti KACC 14956(T) was 17 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain WR-R1Y(T) is separate from other Mucilaginibacter species. On the basis of the data presented, strain WR-R1Y(T) represents a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter calamicampi sp. nov. is proposed. The type strain is WR-R1Y(T) (= KCTC 32214(T) = CCUG 63418(T)).
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea. jhyoon69@skku.edu
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
authors.forename: Sooyeon
authors.initials: S
authors.lastname: Park
authors.forename: Yong-Taek
authors.initials: YT
authors.lastname: Jung
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D041963:Bacteroidetes / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D001482:Base Composition; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008055:Lipids / Q000032:analysis; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D021521:Ribotyping; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D012988:Soil Microbiology; D013045:Species Specificity; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D005227:Fatty Acids; D008055:Lipids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2