title: The papain inhibitor (SPI) of Streptomyces mobaraensis inhibits bacterial cysteine proteases and is an antagonist of bacterial growth.
abstract: A novel papain inhibitory protein (SPI) from Streptomyces mobaraensis was studied to measure its inhibitory effect on bacterial cysteine protease activity (Staphylococcus aureus SspB) and culture supernatants (Porphyromonas gingivalis, Bacillus anthracis). Further, growth of Bacillus anthracis, Staphylococcus aureus, Pseudomonas aeruginosa, and Vibrio cholerae was completely inhibited by 10 μM SPI. At this concentration of SPI, no cytotoxicity was observed. We conclude that SPI inhibits bacterial virulence factors and has the potential to become a novel therapeutic treatment against a range of unrelated pathogenic bacteria.
authors.affiliation: Department of Chemical Engineering and Biotechnology, University of Applied Sciences of Darmstadt, Darmstadt, Germany.
authors.forename: Stephan
authors.initials: S
authors.lastname: Zindel
authors.forename: Wendy E
authors.initials: WE
authors.lastname: Kaman
authors.forename: Sabrina
authors.initials: S
authors.lastname: Fröls
authors.forename: Felicitas
authors.initials: F
authors.lastname: Pfeifer
authors.forename: Anna
authors.initials: A
authors.lastname: Peters
authors.forename: John P
authors.initials: JP
authors.lastname: Hays
authors.forename: Hans-Lothar
authors.initials: HL
authors.lastname: Fuchsbauer
mesh_terms: D001408:Bacillus anthracis / Q000187:drug effects / Q000254:growth & development; D001426:Bacterial Proteins / Q000378:metabolism / Q000494:pharmacology*; D057056:Cysteine Proteases / Q000378:metabolism*; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology*; D010206:Papain / Q000037:antagonists & inhibitors*; D016966:Porphyromonas gingivalis / Q000187:drug effects / Q000254:growth & development; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D013302:Streptomyces / Q000737:chemistry*; D014734:Vibrio cholerae / Q000187:drug effects / Q000254:growth & development
chemical_list: D001426:Bacterial Proteins; D015853:Cysteine Proteinase Inhibitors; C114917:spi protein, actinomycetes; D057056:Cysteine Proteases; D010206:Papain