title: Development of a biologically based fertilizer, incorporating Bacillus megaterium A6, for improved phosphorus nutrition of oilseed rape.
abstract: Sustainable methods with diminished impact on the environment need to be developed for the production of oilseed rape in China and other regions of the world. A biological fertilizer consisting of Bacillus megaterium A6 cultured on oilseed rape meal improved oilseed rape seed yield (P < 0.0001) relative to the nontreated control in 2 greenhouse pot experiments using natural soil. This treatment resulted in slightly greater yield than oilseed rape meal without strain A6 in 1 of 2 experiments, suggesting a role for strain A6 in improving yield. Strain A6 was capable of solubilizing phosphorus from rock phosphate in liquid culture and produced enzymes capable of mineralizing organic phosphorus (acid phosphatase, phytase) in liquid culture and in the biological fertilizer. The biologically based fertilizer, containing strain A6, improved plant phosphorus nutrition in greenhouse pot experiments resulting in significantly greater available phosphorus in natural soil and in significantly greater plant phosphorus content relative to the nontreated control. Seed yield and available phosphorus in natural soil were significantly greater with a synthetic chemical fertilizer treatment, reduced in phosphorus content, than the biological fertilizer treatment, but a treatment containing the biological fertilizer combined with the synthetic fertilizer provided the significantly greatest seed yield, available phosphorus in natural soil, and plant phosphorus content. These results suggest that the biological fertilizer was capable of improving oilseed rape seed yield, at least in part, through the phosphorus-solubilizing activity of B. megaterium A6.
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, People's Republic of China.
authors.forename: Xiaojia
authors.initials: X
authors.lastname: Hu
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Roberts
authors.forename: Lihua
authors.initials: L
authors.lastname: Xie
authors.forename: Jude E
authors.initials: JE
authors.lastname: Maul
authors.forename: Changbing
authors.initials: C
authors.lastname: Yu
authors.forename: Yinshui
authors.initials: Y
authors.lastname: Li
authors.forename: Shujie
authors.initials: S
authors.lastname: Zhang
authors.forename: Xing
authors.initials: X
authors.lastname: Liao
mesh_terms: D010832:6-Phytase; D001410:Bacillus megaterium; D029741:Brassica rapa / Q000254:growth & development* / Q000382:microbiology; D002681:China; D005308:Fertilizers / Q000382:microbiology; D008969:Molecular Sequence Data; D010758:Phosphorus / Q000378:metabolism; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology
chemical_list: D005308:Fertilizers; D012987:Soil; D010758:Phosphorus; D010832:6-Phytase