title: Controlled protein absorption and cell adhesion on polymer-brush-grafted poly(3,4-ethylenedioxythiophene) films.
abstract: Tailoring the surface of biometallic implants with protein-resistant polymer brushes represents an efficient approach to improve the biocompability and mechanical compliance with soft human tissues. A general approach utilizing electropolymerization to form initiating group (-Br) containing poly(3,4-ethylenedioxythiophen)s (poly(EDOT)s) is described. After the conducting polymer is deposited, neutral poly((oligo(ethylene glycol) methacrylate), poly(OEGMA), and zwitterionic poly([2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide), poly(SBMA), brushes are grafted by surface-initiated atom transfer radical polymerization. Quartz crystal microbalance (QCM) experiments confirm protein resistance of poly(OEGMA) and poly(SBMA)-grafted poly(EDOT)s. The protein binding properties of the surface are modulated by the density of polymer brushes, which is controlled by the feed content of initiator-containing monomer (EDOT-Br) in the monomer mixture solution for electropolymerization. Furthermore, these polymer-grafted poly(EDOT)s also prevent cells to adhere on the surface.
authors.affiliation: Yu Initiative Research Unit, RIKEN Advanced Science Institute, Wako, Saitama, Japan. haichaozhao@riken.jp
authors.forename: Haichao
authors.initials: H
authors.lastname: Zhao
authors.forename: Bo
authors.initials: B
authors.lastname: Zhu
authors.forename: Shyh-Chyang
authors.initials: SC
authors.lastname: Luo
authors.forename: Hsing-An
authors.initials: HA
authors.lastname: Lin
authors.forename: Aiko
authors.initials: A
authors.lastname: Nakao
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Yamashita
authors.forename: Hsiao-hua
authors.initials: HH
authors.lastname: Yu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry*; D001966:Bromine / Q000737:chemistry; D002448:Cell Adhesion / Q000502:physiology*; D058266:Dielectric Spectroscopy; D004798:Enzymes / Q000737:chemistry; D006801:Humans; D051379:Mice; D018625:Microscopy, Atomic Force; D041681:NIH 3T3 Cells / Q000166:cytology; D019906:Nuclear Magnetic Resonance, Biomolecular; D011108:Polymers / Q000737:chemistry*; D019736:Prostheses and Implants; D011506:Proteins / Q000737:chemistry*; D058525:Quartz Crystal Microbalance Techniques; D014131:Trace Elements / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D019086:Bridged Bicyclo Compounds, Heterocyclic; D004798:Enzymes; D011108:Polymers; D011506:Proteins; D014131:Trace Elements; C121383:poly(3,4-ethylene dioxythiophene); D001966:Bromine