title: A rapid and low-cost approach to evaluate the allergenicity of herbal injection using HPLC analysis.
abstract: Herbal medicines have ever been thought harmless, but it is obviously not true. Many adverse reports emerged with the development of their popular application in the world. Allergic reactions, especially serious immediate hypersensitivity, frequently occurred when herbal injections were used in clinic and made this ever prevailing agent nearly disappear in China. The aim of this study is to establish a rapid and economical method for the prediction of the allergenicity of herbal injections. Ovalbumin (OVA) and four other herbal injections, in which two of them were well known for their allergenicity, were selected to sensitize and stimulate the animals. Serotonin in the animal serum was detected with HPLC to reflect the anaphylactic response and compared with the other cytokines which could mediate the anaphylaxis, including histamine, IgE and β-hexosaminidase. The results suggest that serotonin can be detected quickly and has good correlation with the other allergy-related cytokines. It is a promising way for predicting the allergenicity of the herbal injections and those complicated natural products.
authors.affiliation: Pharmacy College, Nanjing University of Chinese Medicine, Nanjing 210029, PR China; Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing 210029, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Yu
authors.affiliation: Pharmacy College, Nanjing University of Chinese Medicine, Nanjing 210029, PR China. Electronic address: pgpnjutcm@163.com.
authors.forename: Guoping
authors.initials: G
authors.lastname: Peng
authors.affiliation: Pharmacy College, Nanjing University of Chinese Medicine, Nanjing 210029, PR China.
authors.forename: Cunyu
authors.initials: C
authors.lastname: Li
authors.affiliation: Pharmacy College, Nanjing University of Chinese Medicine, Nanjing 210029, PR China; Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing 210029, PR China.
authors.forename: Baoping
authors.initials: B
authors.lastname: Jiang
authors.affiliation: Pharmacy College, Nanjing University of Chinese Medicine, Nanjing 210029, PR China.
authors.forename: Haokun
authors.initials: H
authors.lastname: Xu
authors.affiliation: Pharmacy College, Nanjing University of Chinese Medicine, Nanjing 210029, PR China.
authors.forename: Ning
authors.initials: N
authors.lastname: Ding
authors.affiliation: Pharmacy College, Nanjing University of Chinese Medicine, Nanjing 210029, PR China.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06520-8026, USA.
authors.forename: John Q
authors.initials: JQ
authors.lastname: Leng
mesh_terms: D000485:Allergens / Q000008:administration & dosage*; D000707:Anaphylaxis / Q000097:blood / Q000139:chemically induced; D000818:Animals; D002681:China; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D016207:Cytokines / Q000097:blood; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage* / Q000009:adverse effects; D006168:Guinea Pigs; D006632:Histamine / Q000097:blood; D006969:Hypersensitivity, Immediate / Q000097:blood / Q000139:chemically induced*; D007073:Immunoglobulin E / Q000097:blood; D007267:Injections / Q000009:adverse effects; D008297:Male; D010047:Ovalbumin; D012701:Serotonin / Q000097:blood*; D001619:beta-N-Acetylhexosaminidases / Q000097:blood
chemical_list: D000485:Allergens; D016207:Cytokines; D004365:Drugs, Chinese Herbal; D012701:Serotonin; D007073:Immunoglobulin E; D006632:Histamine; D010047:Ovalbumin; D001619:beta-N-Acetylhexosaminidases
keywords: Allergic reaction; Herbal medicine; Immediate hypersensitivity; Safety evaluation; Serotonin