title: Antitumor effects of inductive hyperthermia using magnetic ferucarbotran nanoparticles on human lung cancer xenografts in nude mice.
abstract: BACKGROUND
  The effects of inductive hyperthermia on lung cancer have yet to be fully investigated. Magnetic nanoparticles used in inductive hyperthermia are made-to-order and expensive. This study was performed to investigate the use of ferucarbotran in inductive hyperthermia and to clarify whether inductive hyperthermia using ferucarbotran promotes antitumor effects in vivo using a lung cancer cell line.
  
  
  METHODS
  We injected A549 cells subcutaneously into the right thighs of BALB/c nu/nu nude mice. Forty mice with A549 xenografts were then classified into three groups. Group 1 was the control group. All mice in groups 2 and 3 had ferucarbotran injected into their tumors, and mice in group 3 were then subjected to alternating magnetic field irradiation. We evaluated tumor temperature during the hyperthermic procedure, the time course of tumor growth, histologic findings in tumors after hyperthermic treatment, and adverse events.
  
  
  RESULTS
  Intratumor temperature rose rapidly and was maintained at 43°C-45°C for 20 minutes in an alternating magnetic field. Tumor volumes in groups 1 and 2 increased exponentially, but tumor growth in group 3 was significantly suppressed. No severe adverse events were observed. Histologic findings for the tumors in group 3 revealed mainly necrosis.
  
  
  CONCLUSIONS
  Inductive hyperthermia using ferucarbotran is a beneficial and promising approach in the treatment of lung cancer. Ferucarbotran is a novel tool for further development of inductive hyperthermia.
authors.affiliation: Department of Respiratory Medicine, Cellular Transplantation Biology, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Araya
authors.forename: Kazuo
authors.initials: K
authors.lastname: Kasahara
authors.forename: Shingo
authors.initials: S
authors.lastname: Nishikawa
authors.forename: Hideharu
authors.initials: H
authors.lastname: Kimura
authors.forename: Takashi
authors.initials: T
authors.lastname: Sone
authors.forename: Hideo
authors.initials: H
authors.lastname: Nagae
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Ikehata
authors.forename: Isamu
authors.initials: I
authors.lastname: Nagano
authors.forename: Masaki
authors.initials: M
authors.lastname: Fujimura
keywords: alternating magnetic field; ferucarbotran; human use; inductive hyperthermia; lung cancer; magnetic nanoparticles