title: C60-ToF SIMS imaging of frozen hydrated HeLa cells.
abstract: Sample preparation continues to be a major challenge for secondary ion mass spectrometry studies of biological materials. Maintaining the native hydrated state of the material is important for preserving both chemical and spatial information. Here, we discuss a method which combines a sample wash and dry protocol discussed by Bermanet al1 (1)followed by plunge freezing in liquid ethane for a frozen-hydrated analysis of mammalian cells (HeLa). This method allows for the removal of the growth media and maintains the hydrated state of the cells so that they can be prepared frozen-hydrated without the need for a freeze-fracture device. The cells, which were grown on silicon, have been successfully re-grown after the cleaning procedure, confirming that a significant portion of the cells remain undamaged during the wash and dry. Results from preliminary SIMS measurements show that is it possible to detect a large variety of bio-molecular signals, including intact lipids from the plasma membrane in the mass range of 700-900 Da from single cells, with little external water interference at the surface.
authors.affiliation: The Dow Chemical Company, 727 Norristown Road, Spring House, Pennsylvania 19002, United States.
authors.forename: Alan M
authors.initials: AM
authors.lastname: Piwowar
authors.forename: Selda
authors.initials: S
authors.lastname: Keskin
authors.forename: Melissa Ortiz
authors.initials: MO
authors.lastname: Delgado
authors.forename: Kan
authors.initials: K
authors.lastname: Shen
authors.forename: Jonathan J
authors.initials: JJ
authors.lastname: Hue
authors.forename: Ingela
authors.initials: I
authors.lastname: Lanekoff
authors.forename: Andrew G
authors.initials: AG
authors.lastname: Ewing
authors.forename: Nicholas
authors.initials: N
authors.lastname: Winograd