title: LSCluster, a large-scale sequence clustering and aligning software for use in partial identity mapping and splice-variant analysis.
abstract: Many sequence analyses and multiple sequence alignment tools are widely used in biological research and are well described. However, large-scale proteome-wide analysis to identify potential splice-variants, describe the sequence differences compared to a progenitor sequence and cluster those sequences into individual groups for further analysis is a difficult task with the tools available, and a desktop-based, stand-alone search engine with the capabilities to align and cluster thousands of sequences and present the output in a deprecated format has been lacking. We have developed a novel software named LSCluster (Large-Scale CLUSTERing) which allows users to group tens of thousands of sequences based on sequence alignments or partial identity mapping, and can be used specifically for the detection of splicing variants and other pairs of sequences sharing identical fragments. One of the unique features of LSCluster is its ability to display the alignment output as a deprecated string thereby listing only differences in aligned sequences. The software (current version 2.0) is freely available through the PADB (Proteomic Analysis DataBase) initiative at www.PADB.org.
  
  
  CONCLUSIONS
  Large-scale proteome-wide analysis to identify potential splice-variants, describe the sequence differences compared to a progenitor sequence and cluster those sequences into individual groups for further analysis is a difficult task with the tools presently available. This work introduces a desktop-based, stand-alone search engine with the capabilities to align and cluster thousands of sequences and present the output in a deprecated format. We have developed a novel software named LSCluster (Large-Scale CLUSTERing) which allows users to group tens of thousands of sequences based on sequence alignments or partial identity mapping which can be used specifically for the detection of splicing variants and other pairs of sequences sharing identical fragments. One of the unique features of LSCluster is the ability to display the alignment output as a deprecated string listing only differences in aligned sequences. The software (current version 2.0) is freely available through the PADB (Proteomic Analysis DataBase) initiative at www.PADB.org.
authors.affiliation: Biomarkers and Systems Medicine Group, University of Glasgow, Glasgow, UK.
authors.forename: Holger
authors.initials: H
authors.lastname: Husi
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Skipworth
authors.forename: Kenneth C H
authors.initials: KC
authors.lastname: Fearon
authors.forename: James A
authors.initials: JA
authors.lastname: Ross
mesh_terms: D017398:Alternative Splicing / Q000502:physiology*; D030562:Databases, Protein; D016415:Sequence Alignment / Q000379:methods*; D020539:Sequence Analysis, Protein / Q000379:methods*; D012984:Software