title: Gan-Lu-Yin Inhibits Proliferation and Migration of Murine WEHI-3 Leukemia Cells and Tumor Growth in BALB/C Allograft Tumor Model.
abstract: The aim of this study was to explore the antitumor effect of Gan-Lu-Yin (GLY), a traditional Chinese herbal formula, on leukemia. Ethanolic extract of GLY was applied to evaluate its regulatory mechanisms in proliferation, migration, and differentiation of WEHI-3 leukemic cells as well as antitumor effect on BALB/c mice model. The results showed that GLY markedly reduced cell proliferation and migration with induced differentiation of WEHI-3 cells. The expression level of phosphorylated FAK, Akt, ERK1/2, and Rb was decreased p21 expression while level was increased in WEHI-3 treated with GLY. The results of cell cycle analysis revealed that GLY treatment could markedly induce G1 phase arrest and decrease cell population in S phase. Moreover, experimental results demonstrated that GLY decreased the protein expression and enzyme activity of MMP-2 and MMP-9. GLY treatment also reduced WEHI-3 leukemic infiltration in liver and spleen and tumor growth in animal model. Accordingly, GLY demonstrated an inhibitory effect on tumor growth with a regulatory mechanism partially through inhibiting FAK, Akt, and ERK expression in WEHI-3 cells. GLY may provide a promising antileukemic approach in the clinical application.
authors.affiliation: School of Pharmacy, China Medical University, 91 Hsueh-Shih Road, Taichung 40402, Taiwan ; Department of Pharmacy, Da Chien General Hospital, Miaoli 36052, Taiwan.
authors.forename: Fon-Chang
authors.initials: FC
authors.lastname: Liu
authors.forename: Chun-Hsu
authors.initials: CH
authors.lastname: Pan
authors.forename: Ming-Tsung
authors.initials: MT
authors.lastname: Lai
authors.forename: Shu-Jen
authors.initials: SJ
authors.lastname: Chang
authors.forename: Jing-Gung
authors.initials: JG
authors.lastname: Chung
authors.forename: Chieh-Hsi
authors.initials: CH
authors.lastname: Wu