title: Common variants in adiponectin gene are associated with coronary artery disease and angiographical severity of coronary atherosclerosis in type 2 diabetes.
abstract: BACKGROUND
  Adiponectin, an adipokine facilitating insulin action, has antiatherogenic effects. This study investigated whether common single nucleotide polymorphisms (SNPs) in the adiponectin gene influenced plasma adiponectin level and whether they were associated with the risk of coronary artery disease (CAD) and its angiographical severity in type 2 diabetes in Chinese population.
  
  
  METHODS
  11 tagging SNPs were genotyped in 1110 subjects with or without CAD in type 2 diabetes. Variants of adiponectin gene were determined by Taqman polymerase chain reaction method. The plasma adiponectin concentrations were measured by sandwich enzyme-linked immunosorbent assay. The severity and extent of coronary atherosclerosis were assessed using the angiographic Gensini score and Sullivan Extent score.
  
  
  RESULTS
  Among the 11 SNPs, the minor G allele of SNP rs266729 was significantly associated with higher odds of CAD (odds ratio (95% CI) = 1.49 (1.10 - 2.16), P = 0.022) after adjusting for covariates. In stepwise multivariate logistic regression, SNP rs266729 was a significant independent factor of CAD. Multivariate linear regression analysis revealed that rs266729 (β = -0.101, P < 0.0001), rs182052 (β = -0.044, P = 0.0035), and rs1501299 (β = 0.073, P < 0.0001) were significantly associated with adiponectin level, and also indicated that the minor G allele of SNP rs266729 had higher Gensini score (β = 0.139, P < 0.001) and Sullivan Extent score (β = 0.107, P < 0.001). Haplotypes analysis revealed different haplotype distributions in case and control subjects (P = 0.0003), with two common haplotypes GGG and GAG of the rs266729, rs182052, and rs1501299 being associated in heterozygotes with a greater than threefold increase in cardiovascular risk (odds ratio (95% CI)=3.39 (1.83 - 6.30), P = 0.0001).
  
  
  CONCLUSIONS
  In our population, genetic variants in the adiponectin gene influence plasma adiponectin levels, and one of them is a strong determinant of CAD susceptibility and its angiographical severity in type 2 diabetes. This study has provided further evidence for a role of adiponectin in the development of CAD.
authors.affiliation: Department of Cardiology, Capital Medical University, Beijing, China.
authors.forename: Guoxin
authors.initials: G
authors.lastname: Tong
authors.forename: Ningfu
authors.initials: N
authors.lastname: Wang
authors.forename: Jianhang
authors.initials: J
authors.lastname: Leng
authors.forename: Xiaoshan
authors.initials: X
authors.lastname: Tong
authors.forename: Yun
authors.initials: Y
authors.lastname: Shen
authors.forename: Jianmin
authors.initials: J
authors.lastname: Yang
authors.forename: Xianhua
authors.initials: X
authors.lastname: Ye
authors.forename: Liang
authors.initials: L
authors.lastname: Zhou
authors.forename: Yujie
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D052242:Adiponectin / Q000097:blood / Q000235:genetics*; D000368:Aged; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D002681:China; D017023:Coronary Angiography; D003324:Coronary Artery Disease / Q000150:complications / Q000208:ethnology / Q000235:genetics*; D003924:Diabetes Mellitus, Type 2 / Q000150:complications*; D003925:Diabetic Angiopathies / Q000208:ethnology / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D012720:Severity of Illness Index
chemical_list: C499918:ADIPOQ protein, human; D052242:Adiponectin