title: Anti-anaphylactic and anti-inflammatory activities of a bioactive alkaloid from the root bark of Plumeria acutifolia Poir.
abstract: OBJECTIVE
  To investigate the anti-anaphylactic, anti-inflammatory and membrane stabilizing properties of plumerianine (compound 1) isolated from the root bark of Plumeria acutifolia Poir.
  
  
  METHODS
  The anti-anaphylactic activity of compound 1 (10, 25 and 50 mg/kg) was studied by using models such as passive cutaneous anaphylaxis, passive paw anaphylaxis and its anti-inflammatory activity against carrageenin induced paw edema and cotton pellet granuloma in albino rats was also investigated using ketotifen and indomethacin as reference drugs.
  
  
  RESULTS
  A dose-dependent beneficial effect was observed on leakage of evans blue dye in skin challenged with antigen and on paw anaphylaxis induced by antiserum. The compound 1 also exhibited significant (P<0.01) inhibition of rat paw edema and granuloma tissue formation, including significant protection of RBC against the haemolytic effect of hypotonic solution, an indication of membrane-stabilizing activity.
  
  
  CONCLUSIONS
  Anti-anaphylactic activity of compound 1 may be possibly due to inhibition of the release of various inflammatory mediators. Anti-inflammatory activity of compound may be related to the inhibition of the early phase and late phase of inflammatory events.
authors.affiliation: Department of Pharmacognosy, School of Pharmaceutical Sciences, Vels University, Pallavaram, Chennai-117, Tamilnadu, France.
authors.forename: A
authors.initials: A
authors.lastname: Vijayalakshmi
authors.forename: V
authors.initials: V
authors.lastname: Ravichandiran
authors.forename: Malarkodi
authors.initials: M
authors.lastname: Velraj
authors.forename: S
authors.initials: S
authors.lastname: Hemalatha
authors.forename: G
authors.initials: G
authors.lastname: Sudharani
authors.forename: S
authors.initials: S
authors.lastname: Jayakumari
mesh_terms: D000707:Anaphylaxis / Q000188:drug therapy*; D000818:Animals; D018926:Anti-Allergic Agents / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D029062:Apocynaceae / Q000737:chemistry*; D004195:Disease Models, Animal; D004910:Erythrocyte Membrane / Q000187:drug effects; D004912:Erythrocytes; D005260:Female; D039823:Iridoids / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D051379:Mice; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D017208:Rats, Wistar
chemical_list: D018926:Anti-Allergic Agents; D000893:Anti-Inflammatory Agents; D039823:Iridoids; D010936:Plant Extracts; C534199:plumerianine
keywords: Anti-anaphylactic; Anti-inflammatory activity; Inflammatory mediator; Membrane stabilizing; Plumeria acutifolia; Plumerianine