title: Massive bone reconstruction with heat-treated bone graft loaded autologous bone marrow-derived stromal cells and β-tricalcium phosphate composites in canine models.
abstract: Bone marrow-derived stromal cells (BMSCs) contain mesenchymal stem cells that are capable of forming various mesenchymal tissues. We hypothesized that BMSCs and β-tricalcium phosphate (β-TCP) composites would promote the remodeling of large-sized autologous devitalized bone grafts; therefore, the aim of this study was to evaluate the effects of the composites on the remodeling of autologous devitalized bone grafts. Autologous BMSCs cultured in culture medium containing dexamethasone (10(-7)  M) were loaded into porous β-TCP granules under low-pressure. Theses BMSC/TCP composites were put into the bone marrow cavity of autologous heat-treated bone (femoral diaphysis, 65-mm long, 100°C, 30 min) and put back to the harvest site. In the contralateral side, β-TCP without BMSC were used in the same manner as the opposite side as the control. Treatment with the BMSC/TCP composites resulted in a significant increase in thickness, bone mineral density, and matured bone volume of the cortical bone at the center of the graft compared to the control. Histological analysis showed matured regenerated bone in the BMSC loaded group. These results indicate that BMSC/TCP composites facilitated bone regeneration and maturation at the graft site of large-sized devitalized bone. This method could potentially be applied for clinical use in the reconstruction of large bone defects such as those associated with bone tumors.
authors.affiliation: Department of Orthopaedic and Spinal Surgery, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Koyanagi
authors.forename: Keisuke
authors.initials: K
authors.lastname: Ae
authors.forename: Hidetsugu
authors.initials: H
authors.lastname: Maehara
authors.forename: Masato
authors.initials: M
authors.lastname: Yuasa
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Masaoka
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Yamada
authors.forename: Takashi
authors.initials: T
authors.lastname: Taniyama
authors.forename: Masanori
authors.initials: M
authors.lastname: Saito
authors.forename: Yuki
authors.initials: Y
authors.lastname: Funauchi
authors.forename: Toshitaka
authors.initials: T
authors.lastname: Yoshii
authors.forename: Atsushi
authors.initials: A
authors.lastname: Okawa
authors.forename: Shinichi
authors.initials: S
authors.lastname: Sotome
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001861:Bone Regeneration / Q000187:drug effects; D016723:Bone Remodeling / Q000187:drug effects*; D016025:Bone Transplantation; D002130:Calcium Phosphates / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D002516:Ceramics; D003907:Dexamethasone / Q000494:pharmacology; D004195:Disease Models, Animal; D004285:Dogs; D005269:Femur / Q000000981:diagnostic imaging / Q000601:surgery; D006358:Hot Temperature; D008297:Male; D008422:Materials Testing; D055595:Mechanical Phenomena; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects; D011859:Radiography
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D003907:Dexamethasone; C018392:tricalcium phosphate