title: Ectopic Ikaros expression positively correlates with lung cancer progression.
abstract: Ikaros, encoded by the IKZF1 gene, is a pivotal transcription factor whose expression and utilization is dynamically altered during hematopoietic development. However, the molecular mechanisms controlling the transcription of the IKZF1 gene are unclear in lung cancer cell lines. Here we show the role of Ikaros in a cohort of grade IIIA lung cancer patients, with particular emphasis on its relationship with clinical outcomes and expression levels. The expression levels of Ikaros were positively correlated with the prognosis in the lung cancer patients. We also demonstrated that Ikaros expression is ectopically activated in a panel of lung cancer cell lines primarily through demethylation of its promoter. Moreover, gain-of-function experiments revealed that Ikaros inhibits migration and invasion of lung cancer cells in vitro. Our results thus shed light on how Ikaros can act as a lineage competency factor to facilitate lung cancer progression.
authors.affiliation: Department of Lung Cancer, Lung Cancer Center, Tianjin Medical University Cancer Institute and Hospital, Tianjin, 300060, China.
authors.forename: Zhenfa
authors.initials: Z
authors.lastname: Zhang
authors.forename: Zhao
authors.initials: Z
authors.lastname: Xu
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Wang
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Zhi
authors.initials: Z
authors.lastname: Yao
authors.forename: Yanchao
authors.initials: Y
authors.lastname: Mu
authors.forename: Zhenyi
authors.initials: Z
authors.lastname: Ma
authors.forename: Zhe
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000368:Aged; D001483:Base Sequence; D002277:Carcinoma / Q000378:metabolism* / Q000401:mortality; D045744:Cell Line, Tumor; D002465:Cell Movement; D002681:China / Q000453:epidemiology; D019175:DNA Methylation; D018450:Disease Progression; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051740:Ikaros Transcription Factor / Q000235:genetics / Q000378:metabolism*; D008175:Lung Neoplasms / Q000378:metabolism* / Q000401:mortality; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009361:Neoplasm Invasiveness; D011401:Promoter Regions, Genetic
chemical_list: C497239:IKZF1 protein, human; D051740:Ikaros Transcription Factor