title: Cholesterol dependent uptake and interaction of doxorubicin in mcf-7 breast cancer cells.
abstract: Methods of fluorescence spectroscopy and microscopy-including intensity and lifetime (FLIM) images-are used to examine uptake, intracellular location and interaction of the chemotherapeutic drug doxorubicin in MCF-7 human breast cancer cells as a function of cholesterol content. By comparing cells with natural and decreased cholesterol levels after 2 h or 24 h incubation with doxorubicin, we observed that higher fluorescence intensities and possibly shortened fluorescence lifetimes-reflecting increased uptake of the drug and more pronounced drug response-are concomitant with higher membrane fluidity.
authors.affiliation: Institut für Angewandte Forschung, Hochschule Aalen, Anton-Huber Str. 21, 73430 Aalen, Germany. herbert.schneckenburger@htw-aalen.de.
authors.forename: Petra
authors.initials: P
authors.lastname: Weber
authors.forename: Michael
authors.initials: M
authors.lastname: Wagner
authors.forename: Herbert
authors.initials: H
authors.lastname: Schneckenburger
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000493:pharmacokinetics / Q000494:pharmacology; D001693:Biological Transport, Active; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism*; D002784:Cholesterol / Q000378:metabolism*; D004317:Doxorubicin / Q000493:pharmacokinetics* / Q000494:pharmacology*; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D008560:Membrane Fluidity; D008856:Microscopy, Fluorescence; D013050:Spectrometry, Fluorescence
chemical_list: D000903:Antibiotics, Antineoplastic; D004317:Doxorubicin; D002784:Cholesterol