title: Development and validation of a microRNA-based diagnostic assay for classification of renal cell carcinomas.
abstract: Renal cancers account for more than 3% of adult malignancies and cause more than 13,000 deaths per year in the US alone. The four most common types of kidney tumors include the malignant renal cell carcinomas; clear cell, papillary, chromophobe and the benign oncocytoma. These histological subtypes vary in their clinical course and prognosis, and different clinical strategies have been developed for their management. In some kidney tumor cases it can be very difficult for the pathologist to distinguish between tumor types on the basis of morphology and immunohistochemistry (IHC). In this publication we present the development and validation of a microRNA-based assay for classifying primary kidney tumors. The assay, which classifies the four main kidney tumor types, was developed based on the expression of a set of 24 microRNAs. A validation set of 201 independent samples was classified using the assay and analyzed blindly. The assay produced results for 92% of the samples with an accuracy of 95%.
authors.affiliation: Rosetta Genomics Ltd., Rehovot, Israel.
authors.forename: Yael
authors.initials: Y
authors.lastname: Spector
authors.forename: Eddie
authors.initials: E
authors.lastname: Fridman
authors.forename: Shai
authors.initials: S
authors.lastname: Rosenwald
authors.forename: Sofia
authors.initials: S
authors.lastname: Zilber
authors.forename: Yajue
authors.initials: Y
authors.lastname: Huang
authors.forename: Iris
authors.initials: I
authors.lastname: Barshack
authors.forename: Orit
authors.initials: O
authors.lastname: Zion
authors.forename: Heather
authors.initials: H
authors.lastname: Mitchell
authors.forename: Mats
authors.initials: M
authors.lastname: Sanden
authors.forename: Eti
authors.initials: E
authors.lastname: Meiri
mesh_terms: D000328:Adult; D002292:Carcinoma, Renal Cell / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D015331:Cohort Studies; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007668:Kidney / Q000378:metabolism / Q000473:pathology*; D007680:Kidney Neoplasms / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D057089:Pathology, Molecular / Q000379:methods; D015203:Reproducibility of Results
chemical_list: D035683:MicroRNAs