title: Effect of plant phenolic compounds on biofilm formation by Pseudomonas aeruginosa.
abstract: In the natural environment, bacteria predominantly exist in matrix-enclosed multicellular communities associated with various surfaces, referred to as biofilms. Bacteria in biofilms are extremely resistant to antibacterial agents thus causing serious problems for antimicrobial therapy. In this study, we showed that different plant phenolic compounds, at concentrations that did not or weakly suppressed bacterial growth, increased the capacity of Pseudomonas aeruginosa PAO1 to form biofilms. Biofilm formation of P. aeruginosa PAO1 was enhanced 3- to 7-fold under the action of vanillin and epicatechin, and 2- to 2.5-fold in the presence of 4-hydroxybenzoic, gallic, cinnamic, sinapic, ferulic, and chlorogenic acids. At higher concentrations, these compounds displayed an inhibiting effect. Similar experiments carried out for comparison with Agrobacterium tumefaciens C58 showed the same pattern. Vanillin, 4-hydroxybenzoic, and gallic acids at concentrations within the range of 40 to 400 μg/mL increased the production of N-3-oxo-dodecanoyl-homoserine lactone in P. aeruginosa PAO1 which suggests a possible relationship between stimulation of biofilm formation and Las Quorum Sensing system of this bacterium. Using biosensors to detect N-acyl-homoserine lactones (AHL), we demonstrated that the plant phenolics studied did not mimic AHLs.
authors.affiliation: Russian Academy of Sciences, Institute of Molecular Genetics, Moscow, Russia; D. Mendeleev University of Chemical Technology of Russia, Moscow, Russia.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Plyuta
authors.forename: Julia
authors.initials: J
authors.lastname: Zaitseva
authors.forename: Elena
authors.initials: E
authors.lastname: Lobakova
authors.forename: Natalia
authors.initials: N
authors.lastname: Zagoskina
authors.forename: Alexander
authors.initials: A
authors.lastname: Kuznetsov
authors.forename: Inessa
authors.initials: I
authors.lastname: Khmel
mesh_terms: D054742:Acyl-Butyrolactones / Q000378:metabolism; D018441:Biofilms / Q000187:drug effects*; D010636:Phenols / Q000494:pharmacology*; D064209:Phytochemicals / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000502:physiology
chemical_list: D054742:Acyl-Butyrolactones; D010636:Phenols; D064209:Phytochemicals
keywords: Biofilm; N-acyl-homoserine lactones; Pseudomonas aeruginosa; Quorum Sensing; plant phenols