title: High microvessel density in pancreatic ductal adenocarcinoma is associated with high grade.
abstract: The objectives of this work are to study angiogenesis in pancreatic ductal adenocarcinoma using computerized morphometric and image analysis and to compare the microvascular density in intratumoral and peritumoral areas and normal pancreatic tissue. Microvascular density was analyzed in 60 cases of pancreatic ductal adenocarcinoma and 30 samples of normal pancreatic tissue using an avidin-biotin immunoperoxidase technique with an anti-CD31 antibody. Microvascular density (MVD) was analyzed through digital microimaging and computerized analysis. The blood vessel density in the tumor was significantly higher than in peritumoral areas and in normal pancreatic tissue. Well differentiated pancreatic ductal adenocarcinomas contained higher MVD than poorly differentiated carcinomas. In pancreatic adenocarcinoma, MVD is higher than in peritumoral tissue or normal pancreatic tissue.
authors.affiliation: Department of Internal Medicine, Emergency County Hospital Craiova, University of Medicine and Pharmacy Craiova, Petru Rareş 2, Craiova, 200349, Romania. anca_barau@yahoo.com
authors.forename: Anca
authors.initials: A
authors.lastname: Barău
authors.forename: Amparo
authors.initials: A
authors.lastname: Ruiz-Sauri
authors.forename: Gerardo
authors.initials: G
authors.lastname: Valencia
authors.forename: Maria Del Carmen
authors.initials: Mdel C
authors.lastname: Gómez-Mateo
authors.forename: Luis
authors.initials: L
authors.lastname: Sabater
authors.forename: Antonio
authors.initials: A
authors.lastname: Ferrandez
authors.forename: Antonio
authors.initials: A
authors.lastname: Llombart-Bosch
mesh_terms: D000368:Aged; D021441:Carcinoma, Pancreatic Ductal / Q000098:blood supply* / Q000473:pathology*; D005260:Female; D006801:Humans; D007090:Image Interpretation, Computer-Assisted; D008297:Male; D055806:Microvessels / Q000473:pathology; D008875:Middle Aged; D060787:Neoplasm Grading; D009389:Neovascularization, Pathologic / Q000473:pathology*; D010190:Pancreatic Neoplasms / Q000098:blood supply* / Q000473:pathology*