title: Antioxidant activities of Indigofera cassioides Rottl. Ex. DC. using various in vitro assay models.
abstract: OBJECTIVE
  To evaluate the antioxidant potential of methanolic leaf extract of Indigofera cassioides (MEIC) using various in vitro antioxidant assay systems.
  
  
  METHODS
  Antioxidant and free radical scavenging activity of MEIC was assayed by using different in vitro models like ABTS, DPPH, nitric oxide, superoxide, hydrogen peroxide and hydroxyl radical. Reductive ability of the extract was tested by the complex formation with potassium ferricyanide. Further total phenol and flavonoid contents of the crude extract were also determined. Rutin and ascorbic acid were used as standards.
  
  
  RESULTS
  MEIC exhibited potent and concentration dependent free radical scavenging activity in all the tested models. Reductive ability was also found to increase with increase in MEIC concentration. Total phenol and flavonoid content determination showed that the extract is rich in phenols and flavonoids.
  
  
  CONCLUSIONS
  All the results of the in vitro antioxidant assays reveal potent antioxidant and free radical scavenging activity of the leaves of Indigofera cassioides, equivalent to that of standard ascorbic acid and rutin. This potent antioxidant activity may be attributed to its high phenolic and flavonoid contents.
authors.affiliation: Natural Products Laboratory, Swamy Vivekanandha College of Pharmacy, Tiruchengodu, Tamilnadu, India.
authors.forename: R Senthil
authors.initials: RS
authors.lastname: Kumar
authors.forename: B
authors.initials: B
authors.lastname: Rajkapoor
authors.forename: P
authors.initials: P
authors.lastname: Perumal
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry; D005609:Free Radicals / Q000032:analysis / Q000378:metabolism; D029883:Indigofera / Q000737:chemistry*; D008954:Models, Biological; D018384:Oxidative Stress / Q000187:drug effects; D010636:Phenols / Q000032:analysis / Q000737:chemistry; D010851:Picrates; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D013451:Sulfonic Acids
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D005419:Flavonoids; D005609:Free Radicals; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C004931:1,1-diphenyl-2-picrylhydrazyl
keywords: Antioxidant; Free radical scavenging activity; Free radicals; Indigofera cassioides; Oxidative stress; Total reducing power