title: A simple procedure for the preparation of precise spatial multicellular phospholipid polymer hydrogels.
abstract: A precise spatial multicellular polymer hydrogel matrix was prepared by successive assembly of cell-laden hydrogel layers alternated by hydrogel layers without cells based on the spontaneous hydrogel formation between 2 aqueous polymer solutions. The polymers used were a water-soluble 2-methacryloyloxyethyl phosphorylcholine polymer bearing phenylboronic acid groups (PMBV) and poly(vinyl alcohol) (PVA). Each cell-laden layer was deposited as a cell-laden PMBV solution on a PMBV/PVA precursor film. PMBV/PVA multilayer hydrogel was stacked on the top of a cell-laden layer by sequential coating with spinning of the PMBV and PVA solutions. This process allowed the formation of the PMBV/PVA multilayer hydrogel with finely controlled thickness. Finally, we succeeded in cell patterning by using a multilayer hydrogel matrix, forming a sandwich of 2 cell-laden layers separated by a PMBV/PVA multilayer hydrogel. The cells remained alive during the spinning process and maintained their metabolism for at least 24h. This precise spatial multicellular PMBV/PVA hydrogel can be used to examine interactions between many different cells and construct customized microenvironments for multicellular co-cultures.
authors.affiliation: Department of Materials Engineering, School of Engineering, The University of Tokyo 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
authors.forename: Botao
authors.initials: B
authors.lastname: Gao
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Konno
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Ishihara
mesh_terms: D001897:Boronic Acids / Q000737:chemistry; D002470:Cell Survival; D018914:Cells, Immobilized / Q000166:cytology* / Q000502:physiology; D018920:Coculture Techniques; D006367:HeLa Cells; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D020100:Hydrogels; D008689:Methacrylates / Q000737:chemistry*; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry; D014867:Water
chemical_list: D001897:Boronic Acids; D020100:Hydrogels; D008689:Methacrylates; D011108:Polymers; D014867:Water; D010767:Phosphorylcholine; C070638:2-methacryloyloxyethyl phosphorylcholine; D011142:Polyvinyl Alcohol; C010686:benzeneboronic acid