title: Animal model of rapid crystalloid infusion in rats.
abstract: OBJECTIVE
  To describe an animal model of rapid intravenous infusion with different volumes of crystalloid and discuss the clinical findings.
  
  
  METHODS
  Fifty six male Wistar rats were used, divided randomly in seven groups (n = 8). The rats of groups 1 to 6 received lactated Ringer's solution intravenously, in the rate of 25 ml/min, with different volumes proportional to blood volume (BV). The rats of group 0 were submitted to the same procedure, but did not receive the fluid (control group). The data included respiratory rate, heart rate, saturation of peripheral oxygen (SpO(2)) in two times (before and after the infusion), and upshots (respiratory arrest and death). Dunnett's test and ANOVA were used.
  
  
  RESULTS
  The clinical signs significantly changed in the 2, 2.5 and 3 fold BV groups. The respiratory arrest was observed in the 1.5, 2, 2.5 and 3 fold BV groups, but death was present only in 2.5 and 3 fold BV groups.
  
  
  CONCLUSIONS
  The infusion of crystalloid in the same volume of blood volume did not cause significant variation in respiratory and heart rate, saturation of peripheral oxygen and did not induce respiratory arrest. The infusion of a volume of 3 fold blood volume was lethal to all animals.
authors.affiliation: Postgraduate Program in Surgery Bases, Department of Surgery and Orthopedics, Faculty of Medicine of Botucatu, UNESP, Brazil.
authors.forename: Flavio Stillitano
authors.initials: FS
authors.lastname: Orgaes
authors.forename: Fausto Viterbo de
authors.initials: FV
authors.lastname: Oliveira Neto
authors.forename: Flavio Henrique
authors.initials: FH
authors.lastname: Mendes
authors.forename: Renato Florio
authors.initials: RF
authors.lastname: Yabiku
mesh_terms: D000818:Animals; D001810:Blood Volume / Q000187:drug effects; D000077324:Crystalloid Solutions; D005440:Fluid Therapy / Q000379:methods*; D006339:Heart Rate; D007262:Infusions, Intravenous / Q000379:methods; D007552:Isotonic Solutions / Q000008:administration & dosage*; D008297:Male; D023421:Models, Animal; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D056152:Respiratory Rate / Q000187:drug effects
chemical_list: D000077324:Crystalloid Solutions; D007552:Isotonic Solutions