title: [Effect of formaldehyde on acellular-nuclear DNA].
abstract: OBJECTIVE
  To explore the impact of formaldehyde on acellular-nuclear DNA, and initially to establish a new model of acellular nuclear DNA to test adducts.
  
  
  METHODS
  The acellular-nuclear DNA used as a test model, sealed boxes pre-positioned slides fixed with acellular-nuclear DNA. Different concentrations (4%, 1%, 0.25% and 0%) of formaldehyde were added to boxes. Six acellular-nuclear DNA slides were used in each group. DNA damage was measured with comet assay, and statistical analysis of the difference between the groups with SPSS 11.0 software.
  
  
  RESULTS
  DNA damage exposed to formaldehyde in such sequences: 0% = 0.25% > 1% > 4% with significant dose-response relationship.
  
  
  CONCLUSIONS
  Formaldehyde can directly react with acellular DNA, and form adducts and/or DNA-DNA crosslinking, acellular-Nuclear DNA model can be used to detect DNA adducts and cross-linked.
authors.affiliation: Department of Endocrinology, Chinese PLA General Hospital, Beijing 100853, China. Ljyang2828@163.com
authors.forename: Lijuan
authors.initials: L
authors.lastname: Yang
authors.forename: Yueyue
authors.initials: Y
authors.lastname: Wang
authors.forename: Haiyan
authors.initials: H
authors.lastname: Zhu
mesh_terms: D002467:Cell Nucleus / Q000187:drug effects; D020552:Comet Assay; D018736:DNA Adducts / Q000032:analysis*; D004249:DNA Damage / Q000187:drug effects*; D005557:Formaldehyde / Q000633:toxicity*; D006801:Humans; D009153:Mutagens / Q000633:toxicity*
chemical_list: D018736:DNA Adducts; D009153:Mutagens; D005557:Formaldehyde