title: Anticancer property of sediment actinomycetes against MCF-7 and MDA-MB-231 cell lines.
abstract: OBJECTIVE
  To investigate the anticancer property of marine sediment actinomycetes against two different breast cancer cell lines.
  
  
  METHODS
  In vitro anticancer activity was carried out against breast (MCF-7 and MDA-MB-231) cancer cell lines. Partial sequences of the 16s rRNA gene, phylogenetic tree construction, multiple sequence analysis and secondary structure analysis were also carried out with the actinomycetes isolates.
  
  
  RESULTS
  Of the selected five actinomycete isolates, ACT01 and ACT02 showed the IC50 value with (10.13±0.92) and (22.34±5.82) µg/mL concentrations, respectively for MCF-7 cell line at 48 h, but ACT01 showed the minimum (18.54±2.49 µg/mL) level of IC50 value with MDA-MB-231 cell line. Further, the 16s rRNA partial sequences of ACT01, ACT02, ACT03, ACT04 and ACT05 isolates were also deposited in NCBI data bank with the accession numbers of GQ478246, GQ478247, GQ478248, GQ478249 and GQ478250, respectively. The phylogenetic tree analysis showed that, the isolates of ACT02 and ACT03 were represented in group I and III, respectively, but ACT01 and ACT02 were represented in group II. The multiple sequence alignment of the actinomycete isolates showed that, the maximum identical conserved regions were identified with the nucleotide regions of 125 to 221st base pairs, 65 to 119th base pairs and 55, 48 and 31st base pairs. Secondary structure prediction of the 16s rRNA showed that, the maximum free energy was consumed with ACT03 isolate (-45.4 kkal/mol) and the minimum free energy was consumed with ACT04 isolate (-57.6 kkal/mol).
  
  
  CONCLUSIONS
  The actinomycete isolates of ACT01 and ACT02 (GQ478246 and GQ478247) which are isolated from sediment sample can be further used as anticancer agents against breast cancer cell lines.
authors.affiliation: School of Marine Sciences, Department of Oceanography and Coastal Area Studies, Alagappa University, Thondi Campus, Thondi-623 409, Ramanathapuram District, Tamil Nadu, India.
authors.forename: S
authors.initials: S
authors.lastname: Ravikumar
authors.forename: M
authors.initials: M
authors.lastname: Fredimoses
authors.forename: M
authors.initials: M
authors.lastname: Gnanadesigan
mesh_terms: D039903:Actinobacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001483:Base Sequence; D001943:Breast Neoplasms / Q000188:drug therapy*; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D005260:Female; D019015:Geologic Sediments / Q000382:microbiology*; D006801:Humans; D061986:MCF-7 Cells; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D016415:Sequence Alignment; D017423:Sequence Analysis, RNA
chemical_list: D000970:Antineoplastic Agents; D012336:RNA, Ribosomal, 16S
keywords: Actinomycetes; Anticancer property; Anticaner agent; Breast cancer; Cell line; MCF-7; MDA-MB-231; Multiple sequence analysis; Phylogenetic tree; Secondary structure analysis; Sediment