title: Expression and subcellular localization of organophosphate hydrolase in acephate-degrading Pseudomonas sp. strain Ind01 and its use as a potential biocatalyst for elimination of organophosphate insecticides.
abstract: Organophosphate hydrolase (OPH), the product of an organophosphate-degrading (opd) gene cloned from Brevundimonas diminuta, hydrolyses the triester linkage found in neurotoxic organophosphate (OP) insecticides and nerve agents. Despite the fact that OPHs have a broad substrate range, OP compounds with a P-S linkage, such as insecticides like acephate, are poor substrates for the enzyme. Expression of OPH in acephate-utilizing Pseudomonas sp. Ind01 generated a live biocatalyst capable of degrading a wide range of OP insecticides. The heterologously expressed OPH, which is a substrate of twin arginine transport (Tat) pathway, successfully targeted to the membrane of Pseudomonas sp. Ind01. The membrane-associated OPH had a size that coincided with the mature form of OPH (mOPH), suggesting successful processing and targeting of the expressed OPH to the membrane. Pseudomonas sp. Ind01 expressing OPH degraded a variety of OP insecticides besides using acephate as sole carbon source.
  
  
  CONCLUSIONS
  A biocatalyst capable of degrading a wide range of organophosphate (OP) insecticides was generated by expressing an organophosphate degradation gene in Pseudomonas sp. Ind01 involved in mineralization of acephate. The biocatalyst can be used to eliminate a wide range of OP insecticide residues from the environment.
authors.affiliation: Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, India.
authors.forename: A B
authors.initials: AB
authors.lastname: Pinjari
authors.forename: J P
authors.initials: JP
authors.lastname: Pandey
authors.forename: S
authors.initials: S
authors.lastname: Kamireddy
authors.forename: D
authors.initials: D
authors.lastname: Siddavattam
mesh_terms: D001673:Biodegradation, Environmental; D042063:Caulobacteraceae / Q000201:enzymology / Q000235:genetics; D006868:Hydrolysis; D007306:Insecticides / Q000378:metabolism*; D009943:Organophosphorus Compounds / Q000378:metabolism*; D009946:Organothiophosphorus Compounds / Q000378:metabolism; D010573:Pesticide Residues; D063088:Phosphoramides / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics* / Q000378:metabolism; D011549:Pseudomonas / Q000145:classification / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*
chemical_list: D007306:Insecticides; D009943:Organophosphorus Compounds; D009946:Organothiophosphorus Compounds; D010573:Pesticide Residues; D063088:Phosphoramides; C001969:acephate; C045290:phosphorylphosphatase; D010744:Phosphoric Monoester Hydrolases