title: Organelle proteomics: looking at less to see more.
abstract: The recent finding that the human genome comprises between 21 000 and 39 000 genes, a number much lower than expected, has in no way simplified the complexity associated with the understanding of how cells perform their functions. Elucidation of the molecular mechanisms underlying cell functions will require a global knowledge of the expressed proteins, including splice variant products, their post-translational modifications, their subcellular localizations and their assembly into molecular machines as deduced from protein-protein interactions, at any given time during the life of the cell or under any cellular conditions. Current and expected advances in mass spectrometry and bioinformatics might help the realization of these goals in a shorter time than is currently predicted.
authors.affiliation: Departement de pathologie et biologie cellulaire, Université de Montréal, C.P. 6128, Succ. Centre ville, Montreal H3C 3J7, Canada.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Brunet
authors.forename: Pierre
authors.initials: P
authors.lastname: Thibault
authors.forename: Etienne
authors.initials: E
authors.lastname: Gagnon
authors.forename: Paul
authors.initials: P
authors.lastname: Kearney
authors.forename: John J M
authors.initials: JJ
authors.lastname: Bergeron
authors.forename: Michel
authors.initials: M
authors.lastname: Desjardins
mesh_terms: D000818:Animals; D002966:Clathrin / Q000502:physiology; D004705:Endocytosis; D004721:Endoplasmic Reticulum / Q000502:physiology; D055354:Exosomes / Q000737:chemistry; D006801:Humans; D008247:Lysosomes / Q000737:chemistry; D013058:Mass Spectrometry; D015388:Organelles / Q000737:chemistry*; D040901:Proteomics
chemical_list: D002966:Clathrin