title: A robust, rapid, and simple method of producing olfactory receptors using commercial E. coli cell-free systems.
abstract: The first bottleneck in olfactory receptor (OR) studies is producing sufficient quantities of soluble, -functional, and stable receptors. Commercial cell-free in vitro translation systems can be used to produce milligrams of soluble and functional receptors within several hours directly from plasmid DNA. The receptors can be purified using immunoaffinity chromatography and gel filtration, and can be analyzed using gel electrophoresis and with other standard techniques.
authors.affiliation: Center for Bits and Atoms, Massachusetts Institute of Technology, Cambridge, MA, USA.
authors.forename: Karolina
authors.initials: K
authors.lastname: Corin
authors.forename: Brian
authors.initials: B
authors.lastname: Cook
authors.forename: Shuguang
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry; D002850:Chromatography, Gel; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D057809:HEK293 Cells; D006801:Humans; D008863:Microspheres; D018035:Receptors, Odorant / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D012685:Sepharose / Q000737:chemistry; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D018035:Receptors, Odorant; D012685:Sepharose