title: Gemcitabine-loaded albumin nanospheres (GEM-ANPs) inhibit PANC-1 cells in vitro and in vivo.
abstract: With the development of nanotechnology, special attention has been given to the nanomaterial application in tumor treatment. Here, a modified desolvation-cross-linking method was successfully applied to fabricate gemcitabine-loaded albumin nanospheres (GEM-ANPs), with 110 and 406 nm of mean diameter, respectively. The aim of this study was to assess the drug distribution, side effects, and antitumor activity of GEM-ANPs in vivo. The metabolic viability and flow cytometry analysis revealed that both GEM-ANPs, especially 406-nm GEM-ANPs, could effectively inhibit the metabolism and proliferation and promote the apoptosis of human pancreatic carcinoma (PANC-1) in vitro. Intravenous injection of 406-nm GEM-ANPs exhibited a significant increase of gemcitabine in the pancreas, liver, and spleen of Sprague-Dawley rats (p < 0.05). Moreover, no signs of toxic side effects analyzed by blood parameter changes were observed after 3 weeks of administration although a high dose (200 mg/kg) of GEM-ANPs were used. Additionally, in PANC-1-induced tumor mice, intravenous injection of 406-nm GEM-ANPs also could effectively reduce the tumor volume by comparison with free gemcitabine. With these findings, albumin nanosphere-loading approach might be efficacious to improve the antitumor activity of gemcitabine, and the efficacy is associated with the size of GEM-ANPs.
authors.affiliation: Department of Pancreatic Surgery, Huashan Hospital, Fudan University, Shanghai, 200040, China. galleyking@hotmail.com.
authors.forename: Ji
authors.initials: J
authors.lastname: Li
authors.forename: Yang
authors.initials: Y
authors.lastname: Di
authors.forename: Chen
authors.initials: C
authors.lastname: Jin
authors.forename: Deliang
authors.initials: D
authors.lastname: Fu
authors.forename: Feng
authors.initials: F
authors.lastname: Yang
authors.forename: Yongjian
authors.initials: Y
authors.lastname: Jiang
authors.forename: Lie
authors.initials: L
authors.lastname: Yao
authors.forename: Sijie
authors.initials: S
authors.lastname: Hao
authors.forename: Xiaoyi
authors.initials: X
authors.lastname: Wang
authors.forename: Sabin
authors.initials: S
authors.lastname: Subedi
authors.forename: Quanxing
authors.initials: Q
authors.lastname: Ni