title: trans-Resveratrol and ε-viniferin decrease glucose absorption in porcine jejunum and ileum in vitro.
abstract: trans-Resveratrol and ε-viniferin are used as dietary supplements. They are reported to be supportive in preventing arteriosclerosis and diabetes and a previous study could demonstrate an inhibitory potential on sodium-dependent glucose transport (SGLT1) in oocytes und mouse intestinal everted rings (Schulze et al., 2012, Genes Nutr. 6, S61). The in vitro effects of trans-resveratrol and ε-viniferin on intestinal glucose uptake in the porcine small intestines (Sus Scrofa) have not yet been evaluated. It was hypothesized that trans-resveratrol/ε-viniferin may have an adverse effect on porcine intestinal sodium-dependent glucose uptake. The effects on electrogenic small intestinal glucose absorption and sodium-dependent (3)H-glucose uptake in brush border membrane vesicles (BBMV) were evaluated. Pieces of mucosa were mounted into Ussing chambers and were incubated with either trans-resveratrol (0.3 mmol/L), ε-viniferin (0.3 mmol/L), or ethanol. Sodium-dependent glucose absorption into BBMV was measured. (3)H-glucose uptake studies were performed using the same concentrations of the respective substances. SGLT1-mediated glucose absorption was approximately 3-fold higher in ileum compared to jejunum. After preincubation with trans-resveratrol and ε-viniferin, glucose-induced increases of short-circuit currents were significantly decreased. BBMV-studies revealed comparable results and glucose uptake was also significantly decreased. As the glucose transport/uptake was decreased after preincubation with either trans-resveratrol or ε-viniferin this active transport mechanism was directly influenced by inhibiting the SGLT1 transport system.
authors.affiliation: Department of Physiology, University of Veterinary Medicine Hannover, Foundation, 30173 Hannover, Germany.
authors.forename: Maria
authors.initials: M
authors.lastname: Guschlbauer
authors.forename: Stefanie
authors.initials: S
authors.lastname: Klinger
authors.forename: Marion
authors.initials: M
authors.lastname: Burmester
authors.forename: Jana
authors.initials: J
authors.lastname: Horn
authors.forename: Sabine E
authors.initials: SE
authors.lastname: Kulling
authors.forename: Gerhard
authors.initials: G
authors.lastname: Breves
mesh_terms: D000818:Animals; D001572:Benzofurans / Q000494:pharmacology*; D001693:Biological Transport, Active / Q000187:drug effects; D005947:Glucose / Q000378:metabolism*; D007082:Ileum / Q000187:drug effects* / Q000378:metabolism*; D007408:Intestinal Absorption / Q000187:drug effects; D007413:Intestinal Mucosa / Q000378:metabolism; D007583:Jejunum / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D000077185:Resveratrol; D051269:Sodium-Glucose Transporter 1 / Q000378:metabolism; D013267:Stilbenes / Q000494:pharmacology*; D013552:Swine / Q000378:metabolism
chemical_list: D001572:Benzofurans; D051269:Sodium-Glucose Transporter 1; D013267:Stilbenes; C091891:epsilon-viniferin; D005947:Glucose; D000077185:Resveratrol