title: Hyperdry human amniotic membrane is useful material for tissue engineering: physical, morphological properties, and safety as the new biological material.
abstract: Human amniotic membrane (AM) has been used widely as graft biomaterial for a variety of clinical applications. But, there are some persistent problems related to the preparation, storage, and sterilization. To resolve these problems, we developed hyperdry AM (HD-AM) using far-infrared rays, depression of air, and microwaves and then sterilized by γ-ray irradiation. To elucidate the benefit of HD-AM as biological materials, compare with the physical and histological properties of HD-AM with a freeze-dried AM (FD-AM) as typical freeze-dried methods, evaluate the safety of HD-AM in vivo experiment used nude mice, and demonstrate the feasibility of HD-AM transplant in pterygium. The water permeability and the sieving coefficient of HD-AM were significantly lower than that of FD-AM. HD-AM has kept the morphological structure of epithelium and connective tissues. At 18 months after transplanted, single and multilayers of HD-AM in the intraperitoneal cavity was degraded without any infiltrated cells. For clinical treatment, recurrence of pterygium and regrowth of the subconjunctival fibrosis were not observed during the 6-month follow-up periods after the surgery. It was proposed that HD-AM was a safe and effective new biological material for clinical use including treatment for recurrent pterygium.
authors.affiliation: Department of Regenerative Medicine, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan.
authors.forename: Motonori
authors.initials: M
authors.lastname: Okabe
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Kitagawa
authors.forename: Toshiko
authors.initials: T
authors.lastname: Yoshida
authors.forename: Takuma
authors.initials: T
authors.lastname: Suzuki
authors.forename: Hiroki
authors.initials: H
authors.lastname: Waki
authors.forename: Chika
authors.initials: C
authors.lastname: Koike
authors.forename: Etsuko
authors.initials: E
authors.lastname: Furuichi
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Katou
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Nomura
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Uji
authors.forename: Atsushi
authors.initials: A
authors.lastname: Hayashi
authors.forename: Shigeru
authors.initials: S
authors.lastname: Saito
authors.forename: Toshio
authors.initials: T
authors.lastname: Nikaido
mesh_terms: D000650:Amnion / Q000637:transplantation* / Q000648:ultrastructure*; D000818:Animals; D005260:Female; D005612:Freeze Drying; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D011625:Pterygium / Q000601:surgery*; D023822:Tissue Engineering
keywords: biological material; clinical use; freeze-dried human amniotic membrane; hyperdry human amniotic membrane; recurrent pterygium