title: Preparation, purification and regioselective functionalization of protoescigenin--the main aglycone of escin complex.
abstract: A two-step chemical process for controlled degradation of escin, affording a mixture of olean-12-ene sapogenins, was elaborated and scaled up. The main component of the mixture--protoescigenin--was isolated and purified, in the form of its corresponding monohydrate, without resource to chromatographic methods. This material was further converted into the high purity 3,24;16,22-di-O,O-isopropylidene derivative in a validated large scale laboratory process.
authors.affiliation: Pharmaceutical Research Institute, Rydygiera 8, Warszawa 01-793, Poland.
authors.forename: Mariusz M
authors.initials: MM
authors.lastname: Gruza
authors.forename: Kamil
authors.initials: K
authors.lastname: Jatczak
authors.forename: Bogdan
authors.initials: B
authors.lastname: Zagrodzki
authors.forename: Marta
authors.initials: M
authors.lastname: Laszcz
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Koziak
authors.forename: Maura
authors.initials: M
authors.lastname: Malińska
authors.forename: Piotr
authors.initials: P
authors.lastname: Cmoch
authors.forename: Tomasz
authors.initials: T
authors.lastname: Giller
authors.forename: Oliwia
authors.initials: O
authors.lastname: Zegrocka-Stendel
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Woźniak
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Grynkiewicz
mesh_terms: D018360:Crystallography, X-Ray; D004928:Escin / Q000737:chemistry* / Q000302:isolation & purification*; D012502:Sapogenins / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D012502:Sapogenins; D004928:Escin