title: Mobility of acetylcholine receptors in command Helix lucorum neurons in a cellular analog of habituation.
abstract: We investigated the role of the mobility of acetylcholine receptors in the depression of an acetylcholine-induced inward current (ACh-current) of Helix lucorum (a land snail) command neurons of defensive behavior in a cellular analog of habituation. The inhibitors of endocytosis and exocytosis, actin microfilaments and cytoskeleton microtubules, serine/threonine protein kinases (PKA, PKG, calcium calmodulin-dependent PK II, p38 mitogen-activated PK), tyrosine kinases (including Src-family kinases), serine/threonine phosphatases (PP1, PP2A, PP2B, PPM1D), and tyrosine protein phosphatases altered the depression of the ACh-current. A comparison of experimentally calculated curves of the ACh-current of these neurons and those obtained by mathematical modeling revealed the following: (a) ACh-current depression is caused by the reduction in the number of membranous ACh-receptors, which results from the shift in the balance of multidirectional transport processes of receptors toward the predominance of ACh-receptor internalization over their recycling; (b) depression of ACh-current depends on the activity of serine/threonine and tyrosine protein kinases and protein phosphatases, whose one of the main targets is the neuron transport system-actin microfilaments and microtubules of cytoskeleton, as well as motor proteins.
authors.affiliation: Department of Higher Nervous Activity, Biological Faculty, Lomonosov State University, Moscow, 119991, Russia, as_pivovarov@mail.ru.
authors.forename: Arkady S
authors.initials: AS
authors.lastname: Pivovarov
authors.forename: Galina B
authors.initials: GB
authors.lastname: Murzina
authors.forename: Denis A
authors.initials: DA
authors.lastname: Makhnovsky
authors.forename: Mariya S
authors.initials: MS
authors.lastname: Tret'yakova
authors.forename: Natalya A
authors.initials: NA
authors.lastname: Vasil'yeva
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D002118:Calcium / Q000378:metabolism; D006185:Habituation, Psychophysiologic / Q000187:drug effects / Q000502:physiology*; D006372:Helix, Snails / Q000378:metabolism*; D008564:Membrane Potentials / Q000187:drug effects; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D011950:Receptors, Cholinergic / Q000378:metabolism*
chemical_list: D011950:Receptors, Cholinergic; D000109:Acetylcholine; D002118:Calcium