title: Regenerative electronic biosensors using supramolecular approaches.
abstract: A supramolecular interface for Si nanowire FETs has been developed with the aim of creating regenerative electronic biosensors. The key to the approach is Si-NWs functionalized with β-cyclodextrin (β-CD), to which receptor moieties can be attached with an orthogonal supramolecular linker. Here we demonstrate full recycling using the strongest biomolecular system known, streptavidin (SAv)-biotin. The bound SAv and the linkers can be selectively removed from the surface through competitive desorption with concentrated β-CD, regenerating the sensor for repeated use. An added advantage of β-CD is the possibility of stereoselective sensors, and we demonstrate here the ability to quantify the enantiomeric composition of chiral targets.
authors.affiliation: Department of Electrical Engineering, Yale University, New Haven, Connecticut 06520, United States.
authors.forename: Xuexin
authors.initials: X
authors.lastname: Duan
authors.forename: Nitin K
authors.initials: NK
authors.lastname: Rajan
authors.forename: David A
authors.initials: DA
authors.lastname: Routenberg
authors.forename: Jurriaan
authors.initials: J
authors.lastname: Huskens
authors.forename: Mark A
authors.initials: MA
authors.lastname: Reed
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D001710:Biotin / Q000378:metabolism; D019809:Streptavidin / Q000378:metabolism; D013974:Thyroxine / Q000737:chemistry / Q000378:metabolism; D014173:Transistors, Electronic; D047392:beta-Cyclodextrins / Q000378:metabolism
chemical_list: D047392:beta-Cyclodextrins; D001710:Biotin; D019809:Streptavidin; D013974:Thyroxine