title: The Fusarium toxin deoxynivalenol (DON) modulates the LPS induced acute phase reaction in pigs.
abstract: The systemic effects of the Fusarium toxin deoxynivalenol (DON) and of bacterial lipopolysaccharides (LPS) were studied in male castrated pigs (40.4 ± 3.7 kg) infused intravenously with either DON or LPS alone (100 μg DON/kg/h, 7.5 μg/LPS/kg/h), or together (100 μg DON plus 7.5 μg/LPS/kg/h). The Control group received a saline infusion (n=6/treatment, 24h observation period). An additional DON infusion did not exacerbate the clinical signs observed in LPS-infused pigs. For example, rectal temperature climaxed after 4h (40.4 ± 0.2°C) and 5h (40.1 ± 0.3°C), in the LPS and LPS+DON group, respectively. Saline and DON alone did not induce an acute phase reaction as indicated by unaltered plasma levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) while LPS caused a significant rise of both cytokines. TNF-alpha plasma peak concentrations were significantly higher in the LPS compared to the DON+LPS group (94.3 ± 17.2 ng/mL vs. 79.2 ± 15.7 ng/mL) while IL-6 climaxed earlier in the latter group (3h p.i. vs. 2h p.i.). From the tested clinical-chemical plasma characteristics the total bilirubin concentration and the ASAT activity were strongly elevated by the LPS infusion and additionally increased and decreased by DON, respectively. In conclusion, the LPS-induced effects were only marginally modified by DON.
authors.affiliation: Institute of Animal Nutrition, Friedrich-Loeffler-Institute (FLI), Federal Research Institute for Animal Health, Braunschweig, Germany. sven.daenicke@fli.bund.de
authors.forename: Sven
authors.initials: S
authors.lastname: Dänicke
authors.forename: Bianca
authors.initials: B
authors.lastname: Brosig
authors.forename: Susanne
authors.initials: S
authors.lastname: Kersten
authors.forename: Jeannette
authors.initials: J
authors.lastname: Kluess
authors.forename: Stefan
authors.initials: S
authors.lastname: Kahlert
authors.forename: Patricia
authors.initials: P
authors.lastname: Panther
authors.forename: Anne-Kathrin
authors.initials: AK
authors.lastname: Diesing
authors.forename: Hermann-Josef
authors.initials: HJ
authors.lastname: Rothkötter
mesh_terms: D000210:Acute-Phase Reaction / Q000139:chemically induced* / Q000276:immunology; D000818:Animals; D016207:Cytokines / Q000276:immunology; D004347:Drug Interactions; D019446:Endotoxemia / Q000139:chemically induced; D008070:Lipopolysaccharides / Q000633:toxicity*; D008297:Male; D009919:Orchiectomy; D013552:Swine; D014255:Trichothecenes / Q000633:toxicity*
chemical_list: D016207:Cytokines; D008070:Lipopolysaccharides; D014255:Trichothecenes; C007262:deoxynivalenol