title: Association and expression analysis of porcine HNF1A gene related to meat and carcass quality traits.
abstract: The aim of this study was to investigate the association and expression of HNF1A gene as a candidate gene for meat and carcass quality traits in pigs. Statistical analysis revealed that the g.8260 A>G polymorphism significantly associated with pH 24(H), meat percentage and muscle area in the F₂ Duroc × Pietrain (DuPi, n=313) and with pH 24(L), fat area and backfat thickness in the Pietrain (Pi, n=110) population. HNF1A mRNA and protein expressions were higher (p<0.05) in animals with the low post-mortem muscle pH 24(L). The promoter methylation profiling suggested that methylation was not involved on HNF1A expression regulation (p>0.05) in animal with divergent muscle pH. In conclusion, polymorphism in porcine HNF1A gene could be used as a candidate marker to improve the meat and carcass quality traits, with the consideration of breed-specific effect.
authors.affiliation: Institute of Animal Science, Animal Breeding and Husbandry Group, University of Bonn, 53115 Bonn, Germany.
authors.forename: Autchara
authors.initials: A
authors.lastname: Kayan
authors.forename: Muhammad Jasim
authors.initials: MJ
authors.lastname: Uddin
authors.forename: Hakan
authors.initials: H
authors.lastname: Kocamis
authors.forename: Dawit
authors.initials: D
authors.lastname: Tesfaye
authors.forename: Christian
authors.initials: C
authors.lastname: Looft
authors.forename: Ernst
authors.initials: E
authors.lastname: Tholen
authors.forename: Karl
authors.initials: K
authors.lastname: Schellander
authors.forename: Mehmet Ulas
authors.initials: MU
authors.lastname: Cinar
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D001947:Breeding; D004032:Diet; D004041:Dietary Fats / Q000032:analysis; D015870:Gene Expression; D005786:Gene Expression Regulation; D056726:Genetic Association Studies; D051537:Hepatocyte Nuclear Factor 1 / Q000235:genetics* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008460:Meat / Q000032:analysis* / Q000592:standards; D008745:Methylation; D018482:Muscle, Skeletal / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000378:metabolism; D034421:Sus scrofa
chemical_list: D004041:Dietary Fats; D012333:RNA, Messenger; D051537:Hepatocyte Nuclear Factor 1