title: A novel glucose colorimetric sensor based on intrinsic peroxidase-like activity of C60-carboxyfullerenes.
abstract: C60-carboxyfullerene, C60[C(COOH)2]2, has been proven to function as peroxidase mimetics that can catalyze the reaction of peroxidase substrate 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H2O2 to produce a blue color reaction. Kinetic studies further indicate that C60[C(COOH)2]2 has an even higher affinity to TMB than that of the natural enzyme, horseradish peroxidase (HRP). C60[C(COOH)2]2/glucose oxidase (GOx)/TMB system provides a novel colorimetric sensor for glucose and shows good response toward glucose detection over a range of 1.0-40μM with a limit of detection 0.5μM (3σ/slope). Moreover, this sensitive and selective sensor can be successfully applied for the quantitative determination of glucose in human serum. The results indicate that it is a simple, cheap, convenient, highly selective, sensitive, and easy handling colorimetric assay.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Molecular Nanostructure and Nanotechnology, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, PR China.
authors.forename: Ruimin
authors.initials: R
authors.lastname: Li
authors.forename: Mingming
authors.initials: M
authors.lastname: Zhen
authors.forename: Mirong
authors.initials: M
authors.lastname: Guan
authors.forename: Daiqin
authors.initials: D
authors.lastname: Chen
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Zhang
authors.forename: Jiechao
authors.initials: J
authors.lastname: Ge
authors.forename: Ping
authors.initials: P
authors.lastname: Gong
authors.forename: Chunru
authors.initials: C
authors.lastname: Wang
authors.forename: Chunying
authors.initials: C
authors.lastname: Shu
mesh_terms: D001560:Benzidines; D032701:Biomimetics; D015374:Biosensing Techniques / Q000379:methods*; D002264:Carboxylic Acids / Q000737:chemistry*; D002384:Catalysis; D003124:Colorimetry; D037741:Fullerenes / Q000737:chemistry*; D005947:Glucose / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D057230:Limit of Detection; D010084:Oxidation-Reduction; D010544:Peroxidases / Q000737:chemistry
chemical_list: D001560:Benzidines; C416673:C60 carboxyfullerene; D002264:Carboxylic Acids; D037741:Fullerenes; C021758:3,3',5,5'-tetramethylbenzidine; D006861:Hydrogen Peroxide; D010544:Peroxidases; D005947:Glucose