title: Novel protease-based diagnostic devices for detection of wound infection.
abstract: A gelatinase-based device for fast detection of wound infection was developed. Collective gelatinolytic activity in infected wounds was 23 times higher (p ≤ 0.001) than in noninfected wounds and blisters according to the clinical and microbiological description of the wounds. Enzyme activities of critical wounds showed 12-fold elevated enzyme activities compared with noninfected wounds and blisters. Upon incubation of gelatin-based devices with infected wound fluids, an incubation time of 30 minutes led to a clearly visible dye release. A 32-fold color increase was measured after 60 minutes. Both matrix metalloproteinases and elastases contributed to collective gelatinolytic enzyme activity as shown by zymography and inhibition experiments. The metalloproteinase inhibitor 1,10-phenanthroline (targeting matrix metalloproteinases) and the serine protease inhibitor phenylmethlysulfonyl fluoride (targeting human neutrophil elastase) inhibited gelatinolytic activity in infected wound fluid samples by 11-37% and 60-95%, respectively. Staphylococcus aureus and Pseudomonas aeruginosa, both known for gelatinase production, were isolated in infected wound samples.
authors.affiliation: Austrian Center of Industrial Biotechnology, Graz, Austria.
authors.forename: Andrea
authors.initials: A
authors.lastname: Heinzle
authors.forename: Nicole E
authors.initials: NE
authors.lastname: Papen-Botterhuis
authors.forename: Doris
authors.initials: D
authors.lastname: Schiffer
authors.forename: Konstantin P
authors.initials: KP
authors.lastname: Schneider
authors.forename: Barbara
authors.initials: B
authors.lastname: Binder
authors.forename: Michael
authors.initials: M
authors.lastname: Schintler
authors.forename: Ingrid K
authors.initials: IK
authors.lastname: Haaksman
authors.forename: Herman B
authors.initials: HB
authors.lastname: Lenting
authors.forename: Georg M
authors.initials: GM
authors.lastname: Gübitz
authors.forename: Eva
authors.initials: E
authors.lastname: Sigl
mesh_terms: D001419:Bacteria / Q000201:enzymology*; D004867:Equipment Design; D006801:Humans; D008828:Microbiological Techniques / Q000295:instrumentation*; D010447:Peptide Hydrolases / Q000096:biosynthesis*; D015203:Reproducibility of Results; D014946:Wound Infection / Q000175:diagnosis* / Q000201:enzymology / Q000382:microbiology
chemical_list: D010447:Peptide Hydrolases