title: Cytochrome c conjugated to ZnO-MAA nanoparticles: the study of interaction and influence on protein structure.
abstract: Nanoparticle-protein conjugates have potential for numerous applications due to the combination of the properties of both components. In this paper we studied the conjugation of horse heart cytochrome c with ZnO nanoparticles modified by mercaptoacetic acid (MAA) which may be a material with great potential in anticancer therapy as a consequence of synergic effect of both components. Cyt c adsorption to the ZnO-MAA NPs surface was studied by UV-vis spectroscopy and by a dynamic light scattering in various pH. The results indicate that the optimal pH for the association of protein with modified nanoparticles is in range 5.8-8.5 where 90-96% of cytochrome c was assembled on ZnO-MAA nanoparticles. The interaction of proteins with nanoparticles often results in denaturation or loss of protein function. Our observations from UV-vis spectroscopy and circular dichroism performed preserved protein structure after the interaction with modified nanoparticles.
authors.affiliation: CEITEC BUT, Brno University of Technology, Technická 10, 616 69 Brno, Czech Republic.
authors.forename: Michaela
authors.initials: M
authors.lastname: Simšíková
authors.forename: Marián
authors.initials: M
authors.lastname: Antalík
authors.forename: Mária
authors.initials: M
authors.lastname: Kaňuchová
authors.forename: Jiří
authors.initials: J
authors.lastname: Skvarla
mesh_terms: D000327:Adsorption; D000818:Animals; D002942:Circular Dichroism; D045304:Cytochromes c / Q000737:chemistry*; D006736:Horses; D006863:Hydrogen-Ion Concentration; D055767:Immobilized Proteins / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D009206:Myocardium / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D013053:Spectrophotometry; D013864:Thioglycolates / Q000737:chemistry*; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D055767:Immobilized Proteins; D013864:Thioglycolates; C017487:2-mercaptoacetate; D045304:Cytochromes c; D015034:Zinc Oxide