title: Topical application of the lectin Artin M accelerates wound healing in rat oral mucosa by enhancing TGF-β and VEGF production.
abstract: The lectin Artin M has been shown to accelerate the wound-healing process. The aims of this study were to evaluate the effects of Artin M on wound healing in the palatal mucosa of rats and to investigate the effects of Artin M on transforming growth factor beta (TGF-β) and vascular endothelial growth factor (VEGF) secretion by rat gingival fibroblasts. A surgical wound was created on the palatal mucosa of 72 rats divided into three groups according to treatment: C--Control (nontreated), A--Artin M gel, and V--Vehicle. Eight animals per group were sacrificed at 3, 5, and 7 days postsurgery for histology, immunohistochemistry and determination of the levels of cytokines, and growth factors. Gingival fibroblasts were incubated with 2.5 μg/mL of Artin M for 24, 48, and 72 hours. The expression of VEGF and TGF-β was determined by enzyme-linked immunosorbent assay. Histologically, at day 7, the Artin M group showed earlier reepithelialization, milder inflammatory infiltration, and increased collagen fiber formation, resulting in faster maturation of granular tissue than in the other groups (p < 0.05). Artin M-induced cell proliferation in vivo and promoted a greater expression of TGF-β and VEGF in both experiments (p < 0.05). Artin M was effective in healing oral mucosa wounds in rats and was associated with increased TGF-β and VEGF release, cell proliferation, reepithelialization, and collagen deposition and arrangement of fibers.
authors.affiliation: Department of Diagnosis and Surgery, Division of Periodontology, School of Dentistry, UNESP-Univ. Estadual Paulista, São Paulo, Brazil.
authors.forename: Yeon J
authors.initials: YJ
authors.lastname: Kim
authors.forename: Fernanda C
authors.initials: FC
authors.lastname: Carvalho
authors.forename: João A C
authors.initials: JA
authors.lastname: Souza
authors.forename: Pedro C G
authors.initials: PC
authors.lastname: Gonçalves
authors.forename: Andressa V B
authors.initials: AV
authors.lastname: Nogueira
authors.forename: Luis C
authors.initials: LC
authors.lastname: Spolidório
authors.forename: Maria C
authors.initials: MC
authors.lastname: Roque-Barreira
authors.forename: Joni A
authors.initials: JA
authors.lastname: Cirelli
mesh_terms: D000287:Administration, Topical; D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007150:Immunohistochemistry; D037102:Lectins / Q000008:administration & dosage*; D008297:Male; D009061:Mouth Mucosa / Q000187:drug effects / Q000293:injuries* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D016212:Transforming Growth Factor beta / Q000187:drug effects / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000187:drug effects / Q000378:metabolism*; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology
chemical_list: D037102:Lectins; D016212:Transforming Growth Factor beta; D042461:Vascular Endothelial Growth Factor A