title: Expression analysis of jasmonate-responsive lectins in plants.
abstract: The Nicotiana tabacum lectin, also designated Nictaba, is a nucleocytoplasmic carbohydrate-binding protein produced in tobacco leaves after application of specific jasmonates and upon insect herbivory. Here, we describe different techniques by which lectin production can be induced through exogenous jasmonate application on tobacco plants. Furthermore, we elaborate on the assays to detect Nictaba expression at RNA and protein levels as well as on the agglutination assays to identify the lectin activity.
authors.affiliation: Laboratory of Glycobiology and Biochemistry, Department of Molecular Biotechnology, Faculty of Bioscience Engineering, Ghent University, Gent, Belgium.
authors.forename: Nausicaä
authors.initials: N
authors.lastname: Lannoo
authors.forename: Els J M
authors.initials: EJ
authors.lastname: Van Damme
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D015153:Blotting, Western; D003517:Cyclopentanes / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D015870:Gene Expression; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D054883:Oxylipins / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D037121:Plant Lectins / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D037121:Plant Lectins; C072239:methyl jasmonate