title: Biomarkers of oxidative stress study V: ozone exposure of rats and its effect on lipids, proteins, and DNA in plasma and urine.
abstract: Ozone exposure effect on free radical-catalyzed oxidation products of lipids, proteins, and DNA in the plasma and urine of rats was studied as a continuation of the international Biomarker of Oxidative Stress Study (BOSS) sponsored by NIEHS/NIH. The goal was to identify a biomarker for ozone-induced oxidative stress and to assess whether inconsistent results often reported in the literature might be due to the limitations of the available methods for measuring the various types of oxidative products. The time- and dose-dependent effects of ozone exposure on rat plasma lipid hydroperoxides, malondialdehyde, F2-isoprostanes, protein carbonyls, methionine oxidation, and tyrosine- and phenylalanine oxidation products, as well as urinary malondialdehyde and F2-isoprostanes were investigated with various techniques. The criterion used to recognize a marker in the model of ozone exposure was that a significant effect could be identified and measured in a biological fluid seen at both doses at more than one time point. No statistically significant differences between the experimental and the control groups at either ozone dose and time point studied could be identified in this study. Tissue samples were not included. Despite all the work accomplished in the BOSS study of ozone, no available product of oxidation in biological fluid has yet met the required criteria of being a biomarker. The current negative findings as a consequence of ozone exposure are of great importance, because they document that in complex systems, as the present in vivo experiment, the assays used may not provide meaningful data of ozone oxidation, especially in human studies.
authors.affiliation: National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA. Electronic address: Kadiiska@niehs.nih.gov.
authors.forename: Maria B
authors.initials: MB
authors.lastname: Kadiiska
authors.affiliation: Faculty of Medicine, Uppsala University, Uppsala, Sweden; Biochemistry, Molecular Biology and Nutrition Department, University of Auvergne, Clermont-Ferrand, France.
authors.forename: Samar
authors.initials: S
authors.lastname: Basu
authors.affiliation: Department of Microbiology and Immunology, Weill Cornell Medical College, New York, NY, USA.
authors.forename: Nathan
authors.initials: N
authors.lastname: Brot
authors.affiliation: School of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, UK.
authors.forename: Christopher
authors.initials: C
authors.lastname: Cooper
authors.affiliation: Department of Food Science and Nutrition, University of Minnesota, St. Paul, MN, USA.
authors.forename: A
authors.initials: A
authors.lastname: Saari Csallany
authors.affiliation: The Heart Research Institute, University of Sydney, Sydney, Australia.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Davies
authors.affiliation: Rush University Medical Center, Chicago, IL, USA.
authors.forename: Magdalene M
authors.initials: MM
authors.lastname: George
authors.affiliation: Northwest Life Science Specialties, LLC., Vancouver, WA, USA.
authors.forename: Dennis M
authors.initials: DM
authors.lastname: Murray
authors.affiliation: Department of Pharmacology and Medicine, Vanderbilt University, Nashville, TN, USA.
authors.forename: L
authors.initials: L
authors.lastname: Jackson Roberts
authors.affiliation: Children's Hospital Oakland Research Institute, Oakland, CA, USA.
authors.forename: Mark K
authors.initials: MK
authors.lastname: Shigenaga
authors.affiliation: University of Southern California, Los Angeles, CA, USA.
authors.forename: Rajindar S
authors.initials: RS
authors.lastname: Sohal
authors.affiliation: Victor Chang Cardiac Research Institute, Darlinghurst, Australia.
authors.forename: Roland
authors.initials: R
authors.lastname: Stocker
authors.affiliation: Rush University Medical Center, Chicago, IL, USA.
authors.forename: David H
authors.initials: DH
authors.lastname: Van Thiel
authors.affiliation: Otto-von-Guericke-University, Magdeburg, Germany.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Wiswedel
authors.affiliation: United States Environmental Protection Agency, Research Triangle Park, NC, USA.
authors.forename: Gary E
authors.initials: GE
authors.lastname: Hatch
authors.affiliation: National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC, USA.
authors.forename: Ronald P
authors.initials: RP
authors.lastname: Mason
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D004247:DNA / Q000032:analysis* / Q000097:blood / Q000652:urine; D015237:Dinoprost / Q000031:analogs & derivatives / Q000032:analysis; D008054:Lipid Peroxides / Q000032:analysis; D008055:Lipids / Q000032:analysis* / Q000097:blood / Q000652:urine; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D008715:Methionine / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D010126:Ozone / Q000633:toxicity*; D011506:Proteins / Q000032:analysis*; D051381:Rats; D011916:Rats, Inbred F344
chemical_list: D015415:Biomarkers; D008054:Lipid Peroxides; D008055:Lipids; D011506:Proteins; C075750:8-epi-prostaglandin F2alpha; D008315:Malondialdehyde; D010126:Ozone; D004247:DNA; D008715:Methionine; D015237:Dinoprost
keywords: Biomarkers; Oxidative stress; Ozone exposure; Rats