title: Effects of ozone in normal human epidermal keratinocytes.
abstract: Ozone is a tropospheric pollutant that can form at ground level as a result of an interaction between sunlight and hydrocarbon engine emissions. As ozone is an extremely oxidative reaction product, epidermal cells are in the outer layer of defense against ozone. We exposed normal human epidermal keratinocytes (NHEK) to concentrations of ozone that have been measured in cities and assayed for its effects. Hydrogen peroxide and IL-1α levels both increased while ATP levels decreased. We found a decrease in the NAD-dependent histone deacetylase, sirtuin 3. Lastly, we found that ozone increased DNA damage as evaluated by Comet assay. Taken together, our results show increased damage to NHEK that will ultimately impair normal cellular function as a result of an environmentally relevant ozone exposure.
authors.forename: James T
authors.initials: JT
authors.lastname: McCarthy
authors.forename: Edward
authors.initials: E
authors.lastname: Pelle
authors.forename: Kelly
authors.initials: K
authors.lastname: Dong
authors.forename: Krupa
authors.initials: K
authors.lastname: Brahmbhatt
authors.forename: Dan
authors.initials: D
authors.lastname: Yarosh
authors.forename: Nadine
authors.initials: N
authors.lastname: Pernodet
mesh_terms: D000393:Air Pollutants / Q000633:toxicity*; D002478:Cells, Cultured; D000078404:Epidermal Cells; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D010126:Ozone / Q000633:toxicity*; D056566:Sirtuin 3 / Q000378:metabolism
chemical_list: D000393:Air Pollutants; D010126:Ozone; C466093:SIRT3 protein, human; D056566:Sirtuin 3