title: Rapid method demonstration project at four New Jersey marine beaches using real time quantitative Polymerase Chain Reaction (qPCR).
abstract: Real time quantitative Polymerase Chain Reaction (qPCR) was used at four marine bathing beaches in New Jersey as part of a demonstration project to evaluate the potential for use of qPCR as part of a routine beach monitoring program. Split sample analyses for Enterococcus spp. using membrane filtration (MF) and qPCR were performed for 11weeks during the summer of 2011 using swimming advisories based on qPCR results. Comparison of qPCR and MF results from split samples indicated that there was an 82% overall agreement rate between the two methods. Results from the qPCR tests were available by noon the same day of sample collection and swimming advisories were posted on a dedicated website. The qPCR method can be more labor intensive and requires a higher level of training to perform, however, qPCR was able to assess beach water quality in a timelier manner compared to conventional MF techniques.
authors.affiliation: Division of Environmental Science and Assessment, USEPA Region 2, 2890 Woodbridge Ave., Edison, NJ 08837, USA. ferretti.jim@epa.gov
authors.forename: James A
authors.initials: JA
authors.lastname: Ferretti
authors.forename: Hiep V
authors.initials: HV
authors.lastname: Tran
authors.forename: Sarah J
authors.initials: SJ
authors.lastname: Peterson
authors.forename: Virginia
authors.initials: V
authors.lastname: Loftin
mesh_terms: D001493:Bathing Beaches; D004784:Environmental Monitoring / Q000379:methods*; D009515:New Jersey; D016133:Polymerase Chain Reaction; D012623:Seawater / Q000382:microbiology*; D014871:Water Microbiology; D014876:Water Pollution / Q000032:analysis* / Q000706:statistics & numerical data