title: Biomarker discovery and applications for foods and beverages: proteomics to nanoproteomics.
abstract: Foods and beverages have been at the heart of our society for centuries, sustaining humankind - health, life, and the pleasures that go with it. The more we grow and develop as a civilization, the more we feel the need to know about the food we eat and beverages we drink. Moreover, with an ever increasing demand for food due to the growing human population food security remains a major concern. Food safety is another growing concern as the consumers prefer varied foods and beverages that are not only traded nationally but also globally. The 21st century science and technology is at a new high, especially in the field of biological sciences. The availability of genome sequences and associated high-throughput sensitive technologies means that foods are being analyzed at various levels. For example and in particular, high-throughput omics approaches are being applied to develop suitable biomarkers for foods and beverages and their applications in addressing quality, technology, authenticity, and safety issues. Proteomics are one of those technologies that are increasingly being utilized to profile expressed proteins in different foods and beverages. Acquired knowledge and protein information have now been translated to address safety of foods and beverages. Very recently, the power of proteomic technology has been integrated with another highly sensitive and miniaturized technology called nanotechnology, yielding a new term nanoproteomics. Nanoproteomics offer a real-time multiplexed analysis performed in a miniaturized assay, with low-sample consumption and high sensitivity. To name a few, nanomaterials - quantum dots, gold nanoparticles, carbon nanotubes, and nanowires - have demonstrated potential to overcome the challenges of sensitivity faced by proteomics for biomarker detection, discovery, and application. In this review, we will discuss the importance of biomarker discovery and applications for foods and beverages, the contribution of proteomic technology in this process, and a shift towards nanoproteomics to suitably address associated issues. This article is part of a Special Issue entitled: Translational plant proteomics.
authors.affiliation: Research Laboratory for Biotechnology and Biochemistry (RLABB), GPO Box 13265, Kathmandu, Nepal. Electronic address: gkagrawal123@gmail.com.
authors.forename: Ganesh Kumar
authors.initials: GK
authors.lastname: Agrawal
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Timperio
authors.forename: Lello
authors.initials: L
authors.lastname: Zolla
authors.forename: Vipul
authors.initials: V
authors.lastname: Bansal
authors.forename: Ravi
authors.initials: R
authors.lastname: Shukla
authors.forename: Randeep
authors.initials: R
authors.lastname: Rakwal
mesh_terms: D001628:Beverages / Q000032:analysis*; D015415:Biomarkers / Q000032:analysis*; D005504:Food Analysis; D036103:Nanotechnology; D040901:Proteomics / Q000639:trends*
chemical_list: D015415:Biomarkers
keywords: 2-DGE; Beverage; Biomarker; CFU; CLA; CPLL; DLDI; Food; GEPIs; HPLC; MALDI-MS; MS/MS; Nanoproteomics; Nanotechnology; PBMCs; PCA; PR; QCM; ROS; SAW; SERS; SPR; Translational proteomics; colony forming unit; combinatorial peptide ligand library; conjugated linoleic acid; direct laser desorption ionization mass spectroscopy; genetically engineered proteins for inorganics; high-performance liquid chromatography; matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry; peripheral blood mononuclear cells; polyphenol rich; principal component analysis; quartz crystal microbalance; reactive oxygen species; surface acoustic wave; surface enhanced Raman spectroscopy; surface plasmon resonance; tandem mass spectrometry; two-dimensional gel electrophoresis