title: Identifying MicroRNA and mRNA expression profiles in embryonic stem cells derived from parthenogenetic, androgenetic and fertilized blastocysts.
abstract: MicroRNAs (miRNAs) are a class of highly conserved small non-coding RNA molecules that play a pivotal role in several cellular functions. In this study, miRNA and messenger RNA (mRNA) profiles were examined by Illumina microarray in mouse embryonic stem cells (ESCs) derived from parthenogenetic, androgenetic, and fertilized blastocysts. The global analysis of miRNA-mRNA target pairs provided insight into the role of miRNAs in gene expression. Results showed that a total of 125 miRNAs and 2394 mRNAs were differentially expressed between androgenetic ESCs (aESCs) and fertilized ESCs (fESCs), a total of 42 miRNAs and 87 mRNAs were differentially expressed between parthenogenetic ESCs (pESCs) and fESCs, and a total of 99 miRNAs and 1788 mRNAs were differentially expressed between aESCs and pESCs. In addition, a total of 575, 5 and 376 miRNA-mRNA target pairs were observed in aESCs vs. fESCs, pESCs vs. fESCs, and aESCs vs. pESCs, respectively. Furthermore, 15 known imprinted genes and 16 putative uniparentally expressed miRNAs with high expression levels were confirmed by both microarray and real-time RT-PCR. Finally, transfection of miRNA inhibitors was performed to validate the regulatory relationship between putative maternally expressed miRNAs and target mRNAs. Inhibition of miR-880 increased the expression of Peg3, Dyrk1b, and Prrg2 mRNA, inhibition of miR-363 increased the expression of Nfat5 and Soat1 mRNA, and inhibition of miR-883b-5p increased Nfat5, Tacstd2, and Ppapdc1 mRNA. These results warrant a functional study to fully understand the underlying regulation of genomic imprinting in early embryo development.
authors.affiliation: Department of Animal Science, Chungbuk National University, Gaeshin-dong, Cheongju, Chungbuk 361-763, South Korea.
authors.forename: Xiang-Shun
authors.initials: XS
authors.lastname: Cui
authors.forename: Xing-Hui
authors.initials: XH
authors.lastname: Shen
authors.forename: Shao-Chen
authors.initials: SC
authors.lastname: Sun
authors.forename: Sun-Wha
authors.initials: SW
authors.lastname: Cho
authors.forename: Young-Tae
authors.initials: YT
authors.lastname: Heo
authors.forename: Yong-Kook
authors.initials: YK
authors.lastname: Kang
authors.forename: Teruhiko
authors.initials: T
authors.lastname: Wakayama
authors.forename: Nam-Hyung
authors.initials: NH
authors.lastname: Kim
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000378:metabolism*; D002478:Cells, Cultured; D016000:Cluster Analysis; D053595:Embryonic Stem Cells / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D055785:Gene Knockdown Techniques; D008297:Male; D051379:Mice; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000145:classification / Q000235:genetics*; D053652:Nuclear Transfer Techniques; D020411:Oligonucleotide Array Sequence Analysis; D010312:Parthenogenesis; D012333:RNA, Messenger / Q000145:classification / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger