title: [Screening of endophytic fungi from Huperzia serrata for acetylcholinesterase inhibitory activity and its taxonomic identification].
abstract: OBJECTIVE
  To screen out fungus strains with acetylcholinesterase inhibitory activity from Huperzia serrata.
  
  
  METHODS
  Endophytic fungi fermentation products from 59 H. serrata strains were stained with acetylcholinesterase hydrolyzed alpha-naphthaleneacetic ethyl ester and fast blue B salt, and screened for acetylcholinesterase inhibitory activity with thin-layer chromatography-bioautography. Target strains were classified and identified through the sequence analysis on 18s rDNA and 5.8s rDNA combined with morphological characteristics.
  
  
  RESULTS
  Fungus strain LQ2F01 from H. serrata showed positive color reaction in the screening for acetylcholinesterase inhibitory activity. The sequence analysis on 18s rDNA and 5.8s rDNA combined with morphological characteristics showed the strain LQ2F01 belonged to Acremonium.
  
  
  CONCLUSIONS
  Endophytic Fungi LQ2F01 from H. serrata shows identical acetylcholinesterase inhibitory activity with the host plant, which is of great significance to the development of natural medicines and the studies on the relationship between the endophytic gungi and the host plant.
authors.affiliation: The MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. biol2006@126.com
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Wang
authors.forename: Hui-Fang
authors.initials: HF
authors.lastname: Lv
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Hai-Xia
authors.initials: HX
authors.lastname: Hua
authors.forename: Jie-Hua
authors.initials: JH
authors.lastname: Wang
authors.forename: Zhi-Bi
authors.initials: ZB
authors.lastname: Hu
authors.forename: Wan-Kui
authors.initials: WK
authors.lastname: Li
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D000164:Acremonium / Q000235:genetics / Q000378:metabolism; D002800:Cholinesterase Inhibitors / Q000302:isolation & purification / Q000378:metabolism*; D002855:Chromatography, Thin Layer; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D003979:Diazonium Compounds / Q000378:metabolism; D005658:Fungi / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D032501:Huperzia / Q000382:microbiology*; D006868:Hydrolysis; D009280:Naphthaleneacetic Acids / Q000378:metabolism; D010802:Phylogeny; D012337:RNA, Ribosomal, 18S / Q000145:classification / Q000235:genetics; D012340:RNA, Ribosomal, 5.8S / Q000145:classification / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D002800:Cholinesterase Inhibitors; D004271:DNA, Fungal; D004275:DNA, Ribosomal; D003979:Diazonium Compounds; D009280:Naphthaleneacetic Acids; D012337:RNA, Ribosomal, 18S; D012340:RNA, Ribosomal, 5.8S; C016446:Fast Blue BB; C034182:1-naphthaleneacetic acid; D000110:Acetylcholinesterase