title: [Effects of Rheb overexpression in HL-60 and K562 leukemia cell lines].
abstract: mTOR (mammalian target of rapamycin) is the center for cellular activities. It controls many cell activities via inhibiting apoptosis and promoting cell growth. Rheb can activate mTOR signaling pathway and participate in genesis and development of multiple cancers. This study was purposed to explore the underlying role of Rheb in human myeloid leukemia by using the myeloid leukemia cell lines. Two myeloid leukemia cell lines HL-60 and K562 overexpressing Rheb were established with retrovirus containing Rheb. The mRNA and protein expressions of Rheb were determined by Real-Time PCR and Western blot respectively. Cell proliferation rate was examined by CCK-8 assay and apoptosis rate was analyzed using Annexin V and 7-AAD double-staining. The results showed that Rheb was overexpressed in both HL-60 and K562 cell lines. The Rheb overexpression cell lines were successfully established. It is found that overexpression of Rheb could promote cell growth. Furthermore, the overexpression of Rheb could accelerate cells entering into G2/M phase (P < 0.01), while did not affect the apoptosis. It is concluded that Rheb overexpression promotes myeloid leukemia cell proliferation through accelerating cell cycle progression.
authors.affiliation: Chinese Academy of Medical Sciences, Tianjin, China.
authors.forename: Qiao-Zhu
authors.initials: QZ
authors.lastname: Xu
authors.forename: Xiao-Min
authors.initials: XM
authors.lastname: Wang
authors.forename: Fang-Fang
authors.initials: FF
authors.lastname: Wang
authors.forename: Ya-Nan
authors.initials: YN
authors.lastname: Gao
authors.forename: Ying-Chi
authors.initials: YC
authors.lastname: Zhang
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Ju
authors.forename: Tao
authors.initials: T
authors.lastname: Cheng
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Yuan
authors.forename: Han-Zhi
authors.initials: HZ
authors.lastname: Liu
mesh_terms: D002453:Cell Cycle; D049109:Cell Proliferation; D018922:HL-60 Cells; D006801:Humans; D020014:K562 Cells; D020559:Monomeric GTP-Binding Proteins / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism*; D000076205:Ras Homolog Enriched in Brain Protein; D015398:Signal Transduction
chemical_list: D009479:Neuropeptides; C490211:RHEB protein, human; D000076205:Ras Homolog Enriched in Brain Protein; D020559:Monomeric GTP-Binding Proteins