title: Perioperative release of pro-regenerative biochemical signals from human renal allografts subjected to ischemia-reperfusion injury.
abstract: Complement-derived molecules modulate the intensity of renal ischemia-reperfusion injury and may lead to the generation of biochemical signals [such as stromal-derived factor-1 (SDF-1) or sphingosine-1-phosphate (S1P)], which stimulate tissue/organ regeneration after injury. We tested the association between perioperative C5b-9/membrane attack complex (MAC) levels and intensified erythrocyte lysis, and asked whether significant changes in the levels of pro-regenerative substances occur during the early phase of renal allograft reperfusion. Seventy-five recipients were enrolled and divided into the early, slow, and delayed graft function (DGF) groups. Perioperative blood samples were collected from the renal vein during consecutive minutes of reperfusion. Extracellular hemoglobin (eHb), albumin (plasma S1P transporter), 8-iPF2α-III isoprostane, SDF-1 and S1P concentrations were measured. Throughout the reperfusion period, erythrocyte lysis intensified and was most pronounced in the DGF group. However, perioperative eHb levels did not correlate significantly with C5b-9/MAC values, but rather with the intensity of oxidative stress. No significant changes were observed in S1P, its plasma transporter (albumin) or SDF-1 levels, which were relatively low in all groups throughout the reperfusion period. Our study therefore demonstrates that no known biochemical signal for bone marrow-derived stem cell mobilization is released from human renal allografts to the periphery during the early phase of reperfusion.
authors.affiliation: 1Department of Gastroenterology, Pomeranian Medical University, Szczecin, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Błogowski
authors.forename: Barbara
authors.initials: B
authors.lastname: Dolegowska
authors.forename: Marta
authors.initials: M
authors.lastname: Budkowska
authors.forename: Daria
authors.initials: D
authors.lastname: Sałata
authors.forename: Leszek
authors.initials: L
authors.lastname: Domański
authors.forename: Teresa
authors.initials: T
authors.lastname: Starzynska
mesh_terms: D000328:Adult; D064591:Allografts / Q000276:immunology / Q000378:metabolism*; D054377:Chemokine CXCL12 / Q000097:blood; D015938:Complement Membrane Attack Complex / Q000378:metabolism; D051799:Delayed Graft Function / Q000209:etiology / Q000503:physiopathology*; D004912:Erythrocytes / Q000276:immunology; D005260:Female; D006454:Hemoglobins / Q000378:metabolism; D006801:Humans; D028421:Isoprostanes / Q000097:blood; D007668:Kidney / Q000378:metabolism* / Q000473:pathology; D016030:Kidney Transplantation; D008246:Lysophospholipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D059035:Perioperative Period; D012038:Regeneration; D015427:Reperfusion Injury / Q000209:etiology / Q000503:physiopathology*; D013110:Sphingosine / Q000031:analogs & derivatives / Q000097:blood; D055815:Young Adult
chemical_list: C515255:CXCL12 protein, human; D054377:Chemokine CXCL12; D015938:Complement Membrane Attack Complex; D006454:Hemoglobins; D028421:Isoprostanes; D008246:Lysophospholipids; C438786:isoprostane F2alpha III; C060506:sphingosine 1-phosphate; D013110:Sphingosine
keywords: Ischemia–reperfusion injury; kidney regeneration; kidney transplantation