title: [Establishment and application of TaqMan real-time RT-PCR for the detection of hepatitis E virus].
abstract: OBJECTIVE
  To establish a specific TaqMan-based Real-time PCR assay for the detection of hepatitis E virus (HEV).
  
  
  METHODS
  According to the references, primers-probe sets which were located in ORF2, the conservative part of HEV genome were designed and therefore we established a HEV TaqMan real-time RT-PCR assay with great performance of specificity, sensitivity and reproducibility. And then it was used in the detection of HEV RNA in clinical samples.
  
  
  RESULTS
  The HEV Real-time RT-PCR assay established in this study were able to detect HEV RNA with a detection limit of 10 copies/reaction. When the detection of a same sample was repeated for several times, coefficients of variation (CV) was all less than 1.53%. Our data also suggested that there were 1.87 x 10(6)-8.12 x 10(9) RNA copies in 1 ml of the clinical samples.
  
  
  CONCLUSIONS
  The TaqMan-based Real-time PCR assay established in this study was specific and precise for the rapid detection of HEV RNA. It was applied successfully in the pathogen detection of clinical samples.
authors.affiliation: Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.
authors.forename: Qing-Ling
authors.initials: QL
authors.lastname: Meng
authors.forename: Feng
authors.initials: F
authors.lastname: Qiu
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Shen
authors.forename: Sheng-Li
authors.initials: SL
authors.lastname: Bi
mesh_terms: D017931:DNA Primers / Q000235:genetics; D016751:Hepatitis E / Q000821:virology*; D016752:Hepatitis E virus / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D019914:Taq Polymerase / Q000378:metabolism
chemical_list: D017931:DNA Primers; D012367:RNA, Viral; D019914:Taq Polymerase