title: Sinomenine decreases MyD88 expression and improves inflammation-induced joint damage progression and symptoms in rat adjuvant-induced arthritis.
abstract: Sinomenine (SIN) is the active principle of the Chinese medical plant Sinomenium acutum which is widely used for the treatment of rheumatoid arthritis (RA) in China. Recently, several groups indicated that myeloid differentiation primary response protein 88 (MyD88) might be associated with disease progression of RA. Here, we observed the effect of SIN on MyD88 expression and showed its therapeutic role in RA. First, immunohistochemical staining in clinical specimens showed that MyD88 was mainly located in characteristic pathological structures of RA synovial tissues. Second, we found that MyD88 was overexpressed in the synovial tissues of the rats with adjuvant-induced arthritis (AIA). Treatment with SIN markedly decreased the expression of MyD88 in AIA rats. Finally, we provided evidences that SIN suppressed inflammation response and inflammation-induced joint destructive progression and arthritis symptoms in AIA rats. Therefore, SIN is an effective therapeutic agent for RA. Targeting MyD88 signaling may provide new methods for the treatment of RA.
authors.affiliation: Department of Integrated Traditional and Western Medicine, Jinling Hospital, School of Medicine, Second Military Medical University (Shanghai), Nanjing, 210002, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Mu
authors.forename: Ru-Bing
authors.initials: RB
authors.lastname: Yao
authors.forename: Ling-Jie
authors.initials: LJ
authors.lastname: Zhao
authors.forename: Si-Yu
authors.initials: SY
authors.lastname: Shen
authors.forename: Zhi-Ming
authors.initials: ZM
authors.lastname: Zhao
authors.forename: Hui
authors.initials: H
authors.lastname: Cai
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000139:chemically induced / Q000188:drug therapy* / Q000235:genetics; D001172:Arthritis, Rheumatoid / Q000139:chemically induced / Q000188:drug therapy* / Q000235:genetics; D018450:Disease Progression; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use; D007249:Inflammation / Q000188:drug therapy; D007592:Joint Diseases / Q000188:drug therapy*; D008297:Male; D008516:Medicine, Chinese Traditional; D009019:Morphinans / Q000627:therapeutic use*; D053594:Myeloid Differentiation Factor 88 / Q000096:biosynthesis / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013583:Synovial Membrane / Q000378:metabolism; D051195:Toll-Like Receptor 2 / Q000096:biosynthesis; D051197:Toll-Like Receptor 4 / Q000096:biosynthesis
chemical_list: D004365:Drugs, Chinese Herbal; D009019:Morphinans; C507781:Myd88 protein, rat; D053594:Myeloid Differentiation Factor 88; C495343:Tlr2 protein, rat; C495347:Tlr4 protein, rat; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; C009271:sinomenine