title: Fabrication of functional fibronectin patterns by nanosecond excimer laser direct write for tissue engineering applications.
abstract: Laser direct write techniques represent a prospective alternative for engineering a new generation of hybrid biomaterials via the creation of patterns consisting of biological proteins onto practically any type of substrate. In this paper we report on the characterization of fibronectin features obtained onto titanium substrates by UV nanosecond laser transfer. Fourier-transform infrared spectroscopy measurements evidenced no modification in the secondary structure of the post-transferred protein. The molecular weight of the transferred protein was identical to the initial fibronectin, no fragment bands being found in the transferred protein's Western blot migration profile. The presence of the cell-binding domain sequence and the mannose groups within the transferred molecules was revealed by anti-fibronectin monoclonal antibody immunolabelling and FITC-Concanavalin-A staining, respectively. The in vitro tests performed with MC3T3-E1 osteoblast-like cells and Swiss-3T3 fibroblasts showed that the cells' morphology and spreading were strongly influenced by the presence of the fibronectin spots.
authors.affiliation: Equipe de Recherche sur les Relations Matrice Extracellulaire-Cellules, Université de Cergy-Pontoise, 2 Av. Adolphe Chauvin, 95302, Cergy Pontoise, France. sorin.grigorescu@inflpr.ro
authors.forename: S
authors.initials: S
authors.lastname: Grigorescu
authors.forename: M
authors.initials: M
authors.lastname: Hindié
authors.forename: E
authors.initials: E
authors.lastname: Axente
authors.forename: F
authors.initials: F
authors.lastname: Carreiras
authors.forename: K
authors.initials: K
authors.lastname: Anselme
authors.forename: J
authors.initials: J
authors.lastname: Werckmann
authors.forename: I N
authors.initials: IN
authors.lastname: Mihailescu
authors.forename: O
authors.initials: O
authors.lastname: Gallet
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D005353:Fibronectins / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D006801:Humans; D054018:Lasers, Excimer; D051379:Mice; D055616:Microtechnology / Q000295:instrumentation / Q000379:methods; D008954:Models, Biological; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D013499:Surface Properties / Q000528:radiation effects; D041701:Swiss 3T3 Cells; D023822:Tissue Engineering / Q000295:instrumentation*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D005353:Fibronectins