title: Relationship of angiogenesis and microglial activation to seizure-induced neuronal death in the cerebral cortex of Shetland Sheepdogs with familial epilepsy.
abstract: OBJECTIVE
  To determine whether angiogenesis and microglial activation were related to seizure-induced neuronal death in the cerebral cortex of Shetland Sheepdogs with familial epilepsy.
  
  
  METHODS
  Cadavers of 10 Shetland Sheepdogs from the same family (6 dogs with seizures and 4 dogs without seizures) and 4 age-matched unrelated Shetland Sheepdogs.
  
  
  METHODS
  Samples of brain tissues were collected after euthanasia and then fixed in neutral phosphate-buffered 10% formalin and routinely embedded in paraffin. The fixed samples were sectioned for H&E staining and immunohistochemical analysis.
  
  
  RESULTS
  Evidence of seizure-induced neuronal death was detected exclusively in samples of cerebral cortical tissue from the dogs with familial epilepsy in which seizures had been observed. The seizure-induced neuronal death was restricted to tissues from the cingulate cortex and sulci surrounding the cerebral cortex. In almost the same locations as where seizure-induced neuronal death was identified, microvessels appeared longer and more tortuous and the number of microvessels was greater than in the dogs without seizures and control dogs. Occasionally, the microvessels were surrounded by oval to flat cells, which had positive immunohistochemical results for von Willebrand factor. Immunohistochemical results for neurons and glial cells (astrocytes and microglia) were positive for vascular endothelial growth factor, and microglia positive for ionized calcium-binding adapter molecule 1 were activated (ie, had swollen cell bodies and long processes) in almost all the same locations as where seizure-induced neuronal death was detected. Double-label immunofluorescence techniques revealed that the activated microglia had positive results for tumor necrosis factor-α, interleukin-6, and vascular endothelial growth factor receptor 1. These findings were not observed in the cerebrum of dogs without seizures, whether the dogs were from the same family as those with epilepsy or were unrelated to them.
  
  
  CONCLUSIONS
  Signs of angiogenesis and microglial activation corresponded with seizure-induced neuronal death in the cerebral cortex of Shetland Sheepdogs with familial epilepsy. Microglial activation induced by vascular endothelial growth factor and associated proinflammatory cytokine production may accelerate seizure-induced neuronal death in dogs with epilepsy.
authors.affiliation: Department of Veterinary Pathology, Faculty of Agriculture, Tottori University, Tottori, Tottori 680-8553, Japan.
authors.forename: Masashi
authors.initials: M
authors.lastname: Sakurai
authors.forename: Takehito
authors.initials: T
authors.lastname: Morita
authors.forename: Takashi
authors.initials: T
authors.lastname: Takeuchi
authors.forename: Akinori
authors.initials: A
authors.lastname: Shimada
mesh_terms: D000818:Animals; D016923:Cell Death; D002540:Cerebral Cortex / Q000098:blood supply / Q000473:pathology*; D004283:Dog Diseases / Q000235:genetics / Q000473:pathology*; D004285:Dogs; D004827:Epilepsy / Q000235:genetics / Q000473:pathology / Q000662:veterinary*; D017628:Microglia / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000502:physiology