title: Oxidized low-density lipoprotein induces hematopoietic stem cell senescence.
abstract: We have investigated oxidized low-density lipoprotein (ox-LDL) induced senescence in hematopoietic stem cells (HCs). Mouse Sca-1+ HCs were separated and purified using the magnetic activated cell sorting technique. Ox-LDL induced significant senescence in HCs measured by SA-β-Gal staining, and reduced CFU-Mix colony-forming capacity, arresting cells at G0/G1 phase. In agreement with the cell cycle arrest, ox-LDL markedly reduced the expression of CDK4, cyclin D, and cyclin E. As possible contributing factors for cell senescence, ox-LDL also induced cellular oxidative stress and reduced telomerase activity.
authors.affiliation: Department of Histology and Embryology, Laboratory of Stem Cell and Tissue Engineering, Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Xian-Ping
authors.initials: XP
authors.lastname: Zhang
authors.forename: Gui-Hai
authors.initials: GH
authors.lastname: Zhang
authors.forename: Yu-Ying
authors.initials: YY
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wei
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Xu
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Wang
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Wang
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D016922:Cellular Senescence / Q000187:drug effects* / Q000235:genetics; D056741:Cyclin D / Q000235:genetics / Q000378:metabolism; D019927:Cyclin E / Q000235:genetics / Q000378:metabolism; D051358:Cyclin-Dependent Kinase 4 / Q000235:genetics / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D059585:G1 Phase Cell Cycle Checkpoints / Q000187:drug effects* / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects*; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D008297:Male; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects; D061251:Primary Cell Culture; D019098:Telomerase / Q000235:genetics / Q000378:metabolism; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
chemical_list: D015415:Biomarkers; D056741:Cyclin D; D019927:Cyclin E; D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; C495902:Cdk4 protein, mouse; D051358:Cyclin-Dependent Kinase 4; D019098:Telomerase; D001616:beta-Galactosidase
keywords: aging; hematopoietic stem cells; oxidized low-density lipoprotein