title: Effects of a single bout of walking exercise on blood coagulation parameters in obese women.
abstract: Obesity is associated with increased prevalence of thromboembolic events. We aimed to investigate whether obese women might benefit from vigorous aerobic exercise. Forty-two overweight and obese women performed a 30-min walking exercise test (treadmill ergometer) at an intensity of 70% of individual peak oxygen uptake. Blood samples were collected before and immediately after exercise. Thrombelastometry and platelet function measurements were performed on whole blood. Standard coagulation times, thrombin generation curves, markers of thrombin generation, fibrinolytic parameters, plasma levels of pro- and anticoagulatory factors, and microparticle procoagulant activity were determined in platelet-poor plasma samples. Thrombelastometry revealed a significant prolongation of clot formation time (P = 0.037) and a significant deceleration of fibrin build up (alpha angle, P = 0.034) after exercise. Calibrated automated thrombography revealed a significant exercise-induced decrease in endogenous thrombin potential (P = 0.039). Moreover, thrombin formation stopped earlier postexercise, reflected in shortened StartTail (P = 0.046). Significantly elevated tissue-plasminogen activator levels (P = 0.001) indicate an exercise-induced activation of the fibrinolytic system. White blood cell count increased significantly from pre- to postexercise (P = 0.045), indicating a mild exercise-induced leukocytosis. The results of this study demonstrate that vigorous aerobic exercise might be a suitable tool to protect obese women from thrombotic events. We show that a single bout of vigorous aerobic exercise is clearly associated with an activation of the fibrinolytic system and a decreased readiness of the postexercise samples to form a clot and to generate thrombin, the pivotal enzyme of hemostasis.
authors.affiliation: Institute of Physiological Chemistry, Medical University of Graz, Graz, Austria.
authors.forename: Manfred
authors.initials: M
authors.lastname: Lamprecht
authors.forename: Herve
authors.initials: H
authors.lastname: Moussalli
authors.forename: Gerhard
authors.initials: G
authors.lastname: Ledinski
authors.forename: Bettina
authors.initials: B
authors.lastname: Leschnik
authors.forename: Axel
authors.initials: A
authors.lastname: Schlagenhauf
authors.forename: Martin
authors.initials: M
authors.lastname: Koestenberger
authors.forename: Guenter
authors.initials: G
authors.lastname: Polt
authors.forename: Gerhard
authors.initials: G
authors.lastname: Cvirn
mesh_terms: D000328:Adult; D001777:Blood Coagulation / Q000502:physiology*; D015992:Body Mass Index; D015444:Exercise / Q000502:physiology*; D005080:Exercise Test; D005260:Female; D005342:Fibrinolysis; D006339:Heart Rate / Q000502:physiology; D006801:Humans; D007958:Leukocyte Count; D008875:Middle Aged; D009765:Obesity / Q000097:blood* / Q000503:physiopathology*; D010314:Partial Thromboplastin Time; D010973:Platelet Adhesiveness; D010974:Platelet Aggregation; D010979:Platelet Function Tests; D011517:Prothrombin Time; D011659:Pulmonary Gas Exchange; D057185:Sedentary Behavior; D013916:Thrombelastography; D013917:Thrombin / Q000378:metabolism; D016138:Walking / Q000502:physiology*
chemical_list: D013917:Thrombin
keywords: coagulation; exercise; obese women; thrombelastometry; thrombin generation