title: Potential diagnostic value of P16 expression in premalignant and malignant cervical lesions.
abstract: BACKGROUND
  The goal of this study was to evaluate the results of the expression of p16INK4a in normal uterine cervical epithelium, low-grade cervical intraepithelial neoplasia (CIN), high-grade CIN, squamous cell carcinoma (SCC), and adenocarcinoma of the cervix, in order to help draw a distinction between low risk and high risk patients with cervical lesions.
  
  
  METHODS
  [corrected] P16INK4a expression was evaluated by immunohistochemistry in 78 paraffin-embedded tissue samples including 39 normal cervical tissues, 11 low-grade CINs, 11 high-grade CINs, 22 cervical SCCs and 8 cervical adenocarcinomas. Two parameters in immunohistochemical p16 expression were evaluated: percentage of p16-positive cells, and reaction intensity.
  
  
  RESULTS
  The p16INK4a expression rate was 81.8% in low-grade CINs, 91% in high-grade CINs, 90% in SCCs and 75% in cervical adenocarcinomas. 10% of normal cervical samples expressed p16. Moreover, there was a significant relationship between the histological diagnoses and percentage of positive cells and reaction intensity of p16 (p < 0.005). The intensity of the reaction was the best parameter to evaluate the positivity of p16.
  
  
  CONCLUSIONS
  Over-expression of the p16INK4a was typical for dysplastic and neoplastic epithelia of the uterine cervix. However, p16INK4a-negative CINs and carcinomas did exist. Although negative p16INK4a expression does not definitely exclude the patient with cervical lesion from the high-risk group, immunohistochemical study for p16INK4a may be used as a supplementary test for an early diagnosis of cervical cancers.
authors.affiliation: Professor, Department of Pathology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Narges
authors.initials: N
authors.lastname: Izadi-Mood
authors.forename: Kourosh
authors.initials: K
authors.lastname: Asadi
authors.forename: Hadi
authors.initials: H
authors.lastname: Shojaei
authors.forename: Soheila
authors.initials: S
authors.lastname: Sarmadi
authors.forename: Seyed Ali
authors.initials: SA
authors.lastname: Ahmadi
authors.forename: Sanaz
authors.initials: S
authors.lastname: Sani
authors.forename: Leila Hashemi
authors.initials: LH
authors.lastname: Chelavi
keywords: Cervical intraepithelial neoplasia; Human papilloma virus; Immunohistochemistry; P16INK4a