title: Chemical composition, cytotoxic and antimicrobial activity of essential oils from Cassia bakeriana Craib. against aerobic and anaerobic oral pathogens.
abstract: The chemical composition of the essential oils from leaves, bark and wood of Cassia bakeriana Craib. was determined by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Alcohols, aldehydes and fatty acids were the major components in leaf and bark oil, while wood essential oil was rich in fatty acids. Terpenes such as linalool, (E)-nerolidol and phytol were present in low concentrations. The antimicrobial activity against aerobic and anaerobic oral bacteria was evaluated using the microdilution method, as was the cell viability test carried out with Vero cells. The oils from leaves and bark showed high antimicrobial activity, with minimum inhibitory concentrations between 62.5 and 125 µg·mL⁻¹ for most of the tested bacteria, including Streptococcus mutans, the main etiological agent of dental caries. Leaves oil displayed the lowest cytotoxic effect (EC₅₀ of 153 µg·mL⁻¹), while wood oil exhibited the highest toxicity to Vero cells. C. bakeriana oils are thus a source of biologically active compounds against aerobic and anaerobic oral microorganisms. This study is the first report on the chemical composition, antimicrobial activity and cytotoxicity of C. bakeriana.
authors.affiliation: Laboratory of Natural Products and Chromatography, Chemistry Institute, Federal University of Uberlândia, Uberlândia 38408-144, Brazil.
authors.forename: Luís C S
authors.initials: LC
authors.lastname: Cunha
authors.forename: Sérgio A L
authors.initials: SA
authors.lastname: de Morais
authors.forename: Carlos H G
authors.initials: CH
authors.lastname: Martins
authors.forename: Mário M
authors.initials: MM
authors.lastname: Martins
authors.forename: Roberto
authors.initials: R
authors.lastname: Chang
authors.forename: Francisco J T
authors.initials: FJ
authors.lastname: de Aquino
authors.forename: Alberto
authors.initials: A
authors.lastname: de Oliveira
authors.forename: Thaís da S
authors.initials: Tda S
authors.lastname: Moraes
authors.forename: Fabrício C
authors.initials: FC
authors.lastname: Machado
authors.forename: Cláudio V
authors.initials: CV
authors.lastname: da Silva
authors.forename: Evandro A
authors.initials: EA
authors.lastname: do Nascimento
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000494:pharmacology* / Q000633:toxicity; D001420:Bacteria, Aerobic / Q000187:drug effects*; D001421:Bacteria, Anaerobic / Q000187:drug effects*; D002366:Cassia / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D002522:Chlorocebus aethiops; D003731:Dental Caries / Q000382:microbiology; D008826:Microbial Sensitivity Tests; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology* / Q000633:toxicity; D013295:Streptococcus mutans / Q000187:drug effects; D014709:Vero Cells
chemical_list: D000890:Anti-Infective Agents; D009822:Oils, Volatile