title: Antipyretic and anticonvulsant activity of n-hexane fraction of Viola betonicifolia.
abstract: OBJECTIVE
  To investigate the antipyretic and anticonvulsant activities of n-hexane fraction of Viola betonicifolia (V. betonicifolia).
  
  
  METHODS
  The antipyretic effect was scrutinized using brewer's yeast induced pyrexia and anticonvlsion effect was tested using pentylenetetrazol and strychnine induced convulsion in mice.
  
  
  RESULTS
  N-hexane fraction of V. betonicifolia demonstrated highly significant antipyretic activity during various assessment times (1-5 h) when challenged in yeast induced pyrexia test. The effect was in a dose dependent manner with maximum attenuation (82.50%) observed at 300 mg/kg i.p. When tested in pentylenetetrazol induced convulsion test, the 1st stage (Ear and facial twitching) and 2nd stage (Convulsive wave through the body) was 100% protected during 24 h at all the test doses (300, 400 and 500 mg/kg i.p.), while the latency time of remaining stages was significantly increased. The maximum effect was observed by n-hexane fraction of V. betonicifolia at 400 and 500 mg/kg i.p., as the latency time for generalized clonic-tonic seizure (5th stage) was increased up to 25.34 min. However, n-hexane fraction of V. betonicifolia had no protection in strychnine induced convulsion test.
  
  
  CONCLUSIONS
  In conclusion, phytopharmacological studies provide scientific foundation to the folk uses of the plant in the treatment of pyrexia and neurological disorders.
authors.affiliation: Department of Pharmacy University of Peshawar-25120, Peshawar, Pakistan.
authors.forename: Naveed
authors.initials: N
authors.lastname: Muhammad
authors.forename: Muhammad
authors.initials: M
authors.lastname: Saeed
authors.forename: Haroon
authors.initials: H
authors.lastname: Khan
authors.forename: Naila
authors.initials: N
authors.lastname: Raziq
authors.forename: Syed Muhammad Ashhad
authors.initials: SM
authors.lastname: Halimi
authors.forename: Muzaffer
authors.initials: M
authors.lastname: Abass
mesh_terms: D000818:Animals; D000927:Anticonvulsants / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D058633:Antipyretics / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D004195:Disease Models, Animal; D005260:Female; D005334:Fever / Q000188:drug therapy / Q000209:etiology; D006586:Hexanes / Q000737:chemistry*; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D012640:Seizures / Q000139:chemically induced / Q000188:drug therapy; D032413:Viola / Q000737:chemistry*
chemical_list: D000927:Anticonvulsants; D058633:Antipyretics; D006586:Hexanes; D010936:Plant Extracts; C026385:n-hexane
keywords: Anticonvulsant; Antipyretic; N-hexane fraction; Viola betonicifolia