title: Optogenetics in the cerebellum: Purkinje cell-specific approaches for understanding local cerebellar functions.
abstract: The cerebellum consists of the cerebellar cortex and the cerebellar nuclei. Although the basic neuronal circuitry of the cerebellar cortex is uniform everywhere, anatomical data demonstrate that the input and output relationships of the cortex are spatially segregated between different cortical areas, which suggests that there are functional distinctions between these different areas. Perturbation of cerebellar cortical functions in a spatially restricted fashion is thus essential for investigating the distinctions among different cortical areas. In the cerebellar cortex, Purkinje cells are the sole output neurons that send information to downstream cerebellar and vestibular nuclei. Therefore, selective manipulation of Purkinje cell activities, without disturbing other neuronal types and passing fibers within the cortex, is a direct approach to spatially restrict the effects of perturbations. Although this type of approach has for many years been technically difficult, recent advances in optogenetics now enable selective activation or inhibition of Purkinje cell activities, with high temporal resolution. Here we discuss the effectiveness of using Purkinje cell-specific optogenetic approaches to elucidate the functions of local cerebellar cortex regions. We also discuss what improvements to current methods are necessary for future investigations of cerebellar functions to provide further advances.
authors.affiliation: Department of Physiology, The University of Tokyo School of Medicine, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. Electronic address: ttsubota@m.u-tokyo.ac.jp.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Tsubota
authors.forename: Yohei
authors.initials: Y
authors.lastname: Ohashi
authors.forename: Keita
authors.initials: K
authors.lastname: Tamura
mesh_terms: D000818:Animals; D001931:Brain Mapping; D002531:Cerebellum / Q000166:cytology* / Q000502:physiology*; D062308:Optogenetics / Q000379:methods*; D011689:Purkinje Cells / Q000502:physiology*
keywords: Cerebellar cortex; Cerebellar zone; Lentiviral vector; Optogenetics; Purkinje cell