title: Determination of lead in biological samples of children with different physiological consequences using cloud point extraction method.
abstract: In present study, lead (Pb) level in biological samples of children with physiological disorders (liver, bone, and gastrointestinal; age ranged 1-10 years) have been assessed. For comparison purpose, age-matched healthy children were also selected. Cloud point extraction (CPE) was employed for preconcentration of Pb in acid-digested biological samples prior to its determination by flame atomic absorption spectrometry (FAAS). Dithizone (diphenylthiocarbazone) and nonionic surfactant Triton X-114 (TX-114) were used as complexing reagent and extractant, respectively. The effects of several experimental variables on proposed CPE were evaluated. Under the optimum experimental conditions, the observed detection limit (LOD) and the enhancement factor (EF) were 0.08 μg L(-1) and 53, respectively. Relative standard deviation (RSD) of 10 μg L(-1) Pb was 3.4 %. It was observed that children with liver-, bone-, and gastrointestinal-related disorders had three- to fourfold higher Pb level in blood and scalp hair samples.
authors.affiliation: Faculty of Science, Department of Chemistry, Erciyes University, 38039 Kayseri, Turkey. shah_ceac@yahoo.com
authors.forename: Faheem
authors.initials: F
authors.lastname: Shah
authors.forename: Tasneem Gul
authors.initials: TG
authors.lastname: Kazi
authors.forename: Naeem
authors.initials: N
authors.lastname: Ullah
authors.forename: Hassan Imran
authors.initials: HI
authors.lastname: Afridi
mesh_terms: D002138:Calibration; D002648:Child; D002675:Child, Preschool; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007223:Infant; D007854:Lead / Q000032:analysis*; D008024:Ligands; D057230:Limit of Detection; D013054:Spectrophotometry, Atomic; D013696:Temperature
chemical_list: D008024:Ligands; D007854:Lead