title: [Chaperone therapy in the rat model of intracranial glioblastoma].
abstract: Molecular chaperons can effectively activate innate and adaptive anti-tumor immune response. In the model of intracranial glioma C6 in Wistar rats we assessed immunomodulatory activity of recombinant protein Hsp70 in case of local, intratumoral injection. Single intratumoral infusion of chaperone had led to dramatic delay in tumor volume growth (on MRI of rat brain), which was accompanied by increase in survival rates. Incubation of rat spleenocytes with C6 cells elevated the levels of INF-gamma, that shows an immunologically specific T-cell response. With immunohistochemical assay we observed a marked infiltration of the tumor by T-lymphocytes and NK-cells. Thus, purified Hsp70 can efficiently induce innate and adaptive anti-tumor response and could be used as adjuvant in treatment of malignant brain tumors of central nervous system.
authors.forename: M A
authors.initials: MA
authors.lastname: Shevtsov
authors.forename: V A
authors.initials: VA
authors.lastname: Kharatrian
authors.forename: A V
authors.initials: AV
authors.lastname: Pozdniakov
authors.forename: I V
authors.initials: IV
authors.lastname: Romanova
authors.forename: I V
authors.initials: IV
authors.lastname: Guzhova
authors.forename: B A
authors.initials: BA
authors.lastname: Margulis
mesh_terms: D056704:Adaptive Immunity / Q000187:drug effects; D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D001932:Brain Neoplasms / Q000188:drug therapy* / Q000276:immunology*; D004195:Disease Models, Animal; D005909:Glioblastoma / Q000188:drug therapy* / Q000276:immunology*; D018840:HSP70 Heat-Shock Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D007113:Immunity, Innate / Q000187:drug effects; D036502:Infusions, Intralesional; D007371:Interferon-gamma / Q000378:metabolism; D007694:Killer Cells, Natural / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000494:pharmacology; D016019:Survival Analysis; D013601:T-Lymphocytes / Q000187:drug effects; D014407:Tumor Cells, Cultured
chemical_list: D000276:Adjuvants, Immunologic; D000970:Antineoplastic Agents; D018840:HSP70 Heat-Shock Proteins; D011994:Recombinant Proteins; D007371:Interferon-gamma