title: Synthesis of a photocaged tamoxifen for light-dependent activation of Cre-ER recombinase-driven gene modification.
abstract: We report the design of a water-soluble, quaternized tamoxifen photoprobe and demonstrate its application in light-controlled induction of green fluorescent protein expression via a Cre-ER recombinase system.
authors.affiliation: Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA. minlay@stanford.edu
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Inlay
authors.forename: Veronica
authors.initials: V
authors.lastname: Choe
authors.forename: Sophia
authors.initials: S
authors.lastname: Bharathi
authors.forename: Nathaniel B
authors.initials: NB
authors.lastname: Fernhoff
authors.forename: James R
authors.initials: JR
authors.lastname: Baker
authors.forename: Irving L
authors.initials: IL
authors.lastname: Weissman
authors.forename: Seok Ki
authors.initials: SK
authors.lastname: Choi
mesh_terms: D000818:Animals; D002460:Cell Line; D004965:Estrogen Antagonists / Q000737:chemistry* / Q000494:pharmacology / Q000528:radiation effects; D049452:Green Fluorescent Proteins / Q000235:genetics; D019426:Integrases / Q000235:genetics*; D051379:Mice; D009578:Nitrobenzenes / Q000737:chemistry* / Q000528:radiation effects; D011960:Receptors, Estrogen / Q000235:genetics*; D011995:Recombination, Genetic; D013629:Tamoxifen / Q000737:chemistry* / Q000494:pharmacology / Q000528:radiation effects; D014466:Ultraviolet Rays
chemical_list: D004965:Estrogen Antagonists; D009578:Nitrobenzenes; D011960:Receptors, Estrogen; D013629:Tamoxifen; D049452:Green Fluorescent Proteins; C036077:nitrobenzene; C045073:Cre recombinase; D019426:Integrases