title: Secretome of human aortic valves.
abstract: One of the major challenges in cardiovascular medicine is to identify candidate biomarker proteins. Between different proteomics studies, the secretome is a valuable tool in the search for biomarkers locally released by a studied tissue and remains particularly important while working with vascular tissues, since the secreted proteins would be probably shed to the blood. In this chapter, we described a method to validate the origin of secreted proteins in human aortic valves, demonstrating their synthesis and release by the tissue and ruling out blood origin.
authors.affiliation: Laboratorio de Fisiopatología Vascular, Hospital Nacional de Parapléjicos, SESCAM, Toledo, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: de la Cuesta
authors.forename: Gloria
authors.initials: G
authors.lastname: Alvarez-Llamas
authors.forename: Felix
authors.initials: F
authors.lastname: Gil-Dones
authors.forename: Verónica M
authors.initials: VM
authors.lastname: Darde
authors.forename: Enrique
authors.initials: E
authors.lastname: Calvo
authors.forename: Juan Antonio
authors.initials: JA
authors.lastname: López
authors.forename: Fernando
authors.initials: F
authors.lastname: Vivanco
authors.forename: María G
authors.initials: MG
authors.lastname: Barderas
mesh_terms: D001021:Aortic Valve / Q000378:metabolism* / Q000473:pathology; D001024:Aortic Valve Stenosis / Q000378:metabolism* / Q000473:pathology; D002853:Chromatography, Liquid; D017077:Culture Media, Conditioned / Q000737:chemistry*; D006801:Humans; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism; D010449:Peptide Mapping; D059748:Proteolysis; D020543:Proteome / Q000737:chemistry* / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D046509:Tissue Culture Techniques; D014357:Trypsin
chemical_list: D017077:Culture Media, Conditioned; D010446:Peptide Fragments; D020543:Proteome; D014357:Trypsin