title: Comparison of protective and curative potential of Daucus carota root extract on renal ischemia reperfusion injury in rats.
abstract: BACKGROUND
  Daucus carota Linn (Apiaceae), a useful vegetable, is traditionally used in treating kidney and hepatic dysfunctions.
  
  
  OBJECTIVE
  To evaluate the protective and curative potential of D. carota root extract on renal ischemia reperfusion injury in rats.
  
  
  METHODS
  Wistar rats were selected with 8 + 8 groups (n = 6). Renal pedicles of rats were occluded for 45 min and allowed for reperfusion period. In protective and curative studies, 14 days prior and 14 days after the induction of ischemia/reperfusion (I/R), rats received petroleum ether extract (PEE 250 and 500 mg/kg), fractional methanol extract (FME 250 and 500 mg/kg) and direct methanol extract (DME 250 and 500 mg/kg) of Daucus carota root, orally, once daily.
  
  
  RESULTS
  PEE at a dose of 500 mg/kg significantly (p < 0.001) reduced the levels of serum creatinine (0.853-3.090 mg/dl), uric acid (1.300-3.500 mg/dl) and urea (58.26-132.00 mg/dl) compared to disease control. FME at a dose of 500 mg/kg body weight significantly (p < 0.001) reduced the levels of serum creatinine (0.960-3.090 mg/dl), uric acid (1.700-3.500 mg/dl) and urea (77.17-132.00 mg/dl) compared to disease control. DME at a dose of 500 mg/kg body weight significantly (p < 0.001) reduced the levels of serum creatinine (1.173-3.090 mg/dl), uric acid (2.267-3.500 mg/dl) and urea (84.75-132.00 mg/dl) compared to disease control.
  
  
  CONCLUSIONS
  Findings demonstrate that postconditioning with the D. carota root extract significantly improves kidney function in I/R rats.
authors.affiliation: Department of Pharmacology, Siddhartha Institute of Pharmacy, Dehradun, Uttarakhand, India.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Afzal
authors.forename: Imran
authors.initials: I
authors.lastname: Kazmi
authors.forename: Rupinder
authors.initials: R
authors.lastname: Kaur
authors.forename: Aftab
authors.initials: A
authors.lastname: Ahmad
authors.forename: Mohammad
authors.initials: M
authors.lastname: Pravez
authors.forename: Firoz
authors.initials: F
authors.lastname: Anwar
mesh_terms: D058186:Acute Kidney Injury / Q000188:drug therapy* / Q000503:physiopathology / Q000517:prevention & control; D000284:Administration, Oral; D000818:Animals; D003404:Creatinine / Q000097:blood; D018552:Daucus carota / Q000737:chemistry*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D057775:Ischemic Postconditioning / Q000379:methods; D019194:Ischemic Preconditioning / Q000379:methods; D007677:Kidney Function Tests; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D018517:Plant Roots; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000150:complications / Q000188:drug therapy*; D014508:Urea / Q000097:blood; D014527:Uric Acid / Q000097:blood
chemical_list: D010936:Plant Extracts; D014527:Uric Acid; D014508:Urea; D003404:Creatinine