title: Label-free detection of single-stranded DNA binding protein based on a cantilever array.
abstract: We report a simple and sensitive method for label-free detection of single-stranded DNA-binding protein (SSBP) based on an array of microfabricated cantilevers. The single-stranded DNA (ssDNA) was immobilized on the surface of the sensing cantilevers to detect SSBP, while the reference cantilevers were modified with 6-mercapto-1-hexanol to detect any unwanted cantilever deflection. The differential deflection signals that reveal specific SSBP-ssDNA binding have been found to depend on the SSBP concentration. Using the cantilever array sensor we can detect SSBP in the concentration range from 0.01 to 7 μg mL(-1). Other proteins, such as thrombin or bovine serum albumin induced no significant deflection of the cantilevers. Our results show the potential for the application of cantilever array sensor system as a powerful tool to detect proteins with high sensitivity and specificity.
authors.affiliation: Department of Chemistry, Nanchang University, Nanchang 330031, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Hou
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Bai
authors.forename: Chunyan
authors.initials: C
authors.lastname: Xing
authors.forename: Baoping
authors.initials: B
authors.lastname: Lu
authors.forename: Jinhui
authors.initials: J
authors.lastname: Hao
authors.forename: Xi
authors.initials: X
authors.lastname: Ke
authors.forename: Ningyu
authors.initials: N
authors.lastname: Gu
authors.forename: Bailin
authors.initials: B
authors.lastname: Zhang
authors.forename: Jilin
authors.initials: J
authors.lastname: Tang
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004277:DNA, Single-Stranded / Q000737:chemistry; D004268:DNA-Binding Proteins / Q000032:analysis* / Q000737:chemistry; D004867:Equipment Design; D000441:Hexanols / Q000737:chemistry; D040081:Protein Array Analysis / Q000295:instrumentation / Q000379:methods*; D011485:Protein Binding; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013917:Thrombin / Q000737:chemistry
chemical_list: C503488:6-mercapto-1-hexanol; D004277:DNA, Single-Stranded; D004268:DNA-Binding Proteins; D000441:Hexanols; D013438:Sulfhydryl Compounds; D012710:Serum Albumin, Bovine; D013917:Thrombin