title: In vitro enzymatic degradation of a biological tissue fixed by alginate dialdehyde.
abstract: Biological tissues must be chemically fixed before they can be implanted in humans as tissue engineering scaffolds. To provide an ideal tissue engineering scaffold material, which is biodegradable and cytocompatible, a novel crosslinking agent, alginate dialdehyde (ADA), was employed to fix biological tissues by our group. The study mainly investigated the enzymatic degradation of ADA fixed biological tissues in vitro. Glutaraldehyde, the most commonly used crosslinking agent for biological tissue fixation, was employed as a control. The results suggested that, the ADA fixation could enhance the resistance against enzymatic degradation of biological tissues effectively. Meanwhile, compared to glutaraldehyde-fixed tissues, the ADA-fixed tissues could also degrade gradually over time. Moreover, the ADA crosslinking reagent itself had a stimulatory effect on cell proliferation when at an appropriate concentration. The results obtained in this study demonstrate that ADA fixation might provide a successful example of the biodegradable scaffold materials in tissue engineering.
authors.affiliation: College of Polymer Science and Engineering, Sichuan University, Chengdu, Sichuan Province 610065, PR China.
authors.forename: Yuanting
authors.initials: Y
authors.lastname: Xu
authors.forename: Chengcheng
authors.initials: C
authors.lastname: Huang
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Xixun
authors.initials: X
authors.lastname: Yu
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Peng
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Gu
authors.forename: Yaping
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000447:Aldehydes / Q000737:chemistry*; D000464:Alginates / Q000737:chemistry*; D000818:Animals; D001011:Aorta; D001672:Biocompatible Materials; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D017364:Collagenases / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D000431:Ethanol / Q000737:chemistry; D051379:Mice; D010504:Periodic Acid / Q000737:chemistry; D013552:Swine; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
chemical_list: D000447:Aldehydes; D000464:Alginates; D001672:Biocompatible Materials; D003432:Cross-Linking Reagents; D010504:Periodic Acid; D000431:Ethanol; C009288:metaperiodate; D017364:Collagenases; C417249:collagenase 1