title: Thyroid-stimulating hormone and free thyroxine on the ADVIA Centaur immunoassay system: a multicenter assessment of analytical performance.
abstract: OBJECTIVE
  We assessed the analytical performance of the TSH and FT4 assays on ADVIA Centaur in a multicenter national evaluation.
  
  
  METHODS
  A precision study and a method comparison were performed. Reference values stated by the manufacturer were checked from 379 normal subjects.
  
  
  RESULTS
  For TSH and FT4, the intra-assay CVs were below 2.3 and 5.2%, respectively, and the inter-assay CVs below 4.4% and 7.2%, respectively. Therefore, the precision and reproducibility were acceptable. Bland-Altman bias plots revealed good correlation and agreement with Cobas assays. TSH and FT4 data yielded reference ranges of 0.64-3.24 mIU/L and 10.5-18.9 pmol/L, respectively.
  
  
  CONCLUSIONS
  These assays demonstrate reliable characteristics. The reference ranges obtained can be used for interpretation of thyroid function.
authors.affiliation: Laboratoire d'Hormonologie, Hôpitaux Universitaires de Strasbourg, 1 place de l'Hôpital, 67091 Strasbourg Cedex, France. nathalie.reix@chru-strasbourg.fr
authors.forename: Nathalie
authors.initials: N
authors.lastname: Reix
authors.forename: Catherine
authors.initials: C
authors.lastname: Massart
authors.forename: Michèle
authors.initials: M
authors.lastname: d'Herbomez
authors.forename: Françoise
authors.initials: F
authors.lastname: Gasser
authors.forename: Béatrice
authors.initials: B
authors.lastname: Heurtault
authors.forename: Arnaud
authors.initials: A
authors.lastname: Agin
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation* / Q000592:standards; D008163:Luminescent Measurements / Q000295:instrumentation; D008297:Male; D008875:Middle Aged; D012016:Reference Values; D015203:Reproducibility of Results; D013960:Thyroid Function Tests / Q000295:instrumentation; D013972:Thyrotropin / Q000097:blood*; D013974:Thyroxine / Q000097:blood*
chemical_list: D013972:Thyrotropin; D013974:Thyroxine
keywords: Evaluation; Free thyroxine; Reference values; Thyroid-stimulating hormone