title: Greener liquid chromatography using a guard column with micellar mobile phase for separation of some pharmaceuticals and determination of parabens.
abstract: In this research, a greener chromatography employing a short column, Zorbax SB C18 cartridge (12.5 × 4.6 mm, 5 μm) commonly used as a guard column in a reverse phase high performance liquid chromatography (RP-HPLC), was utilized as the analytical column in conjunction with a more eco-friendly micellar mobile phase of sodium dodecyl sulfate (SDS) for separation tertiary mixtures of local anesthetics and antihistamines; and binary mixture of colds drugs; and quaternary mixture of some parabens with different separation conditions. The chromatographic behavior of these analytes was studied to demonstrate separation efficiency of this guard column in a micellar mobile phase. Moreover, this column and SDS mobile phase was exploited for determination of parabens in 64 samples of cosmetic product, both those that were produced locally in the community and those that were commercially manufactured. Linear calibration graphs of the parabens as detected at 254 nm were obtained in the range of 1-100 μmol L(-1) with R(2)>0.9990. Percentage recoveries were 92.4-109.2 with %RSD<3, and the limit of detection and quantitation were 0.04-0.10 and 0.20-0.80 μmol L(-1), respectively. This analytical system is not only greener but also faster and employing simpler sample preparation than a conventional liquid chromatographic system.
authors.affiliation: Department of Chemistry, Faculty of Science and Technology, Thammasat University, Pathum Thani 12120, Thailand. napaporn@tu.ac.th
authors.forename: Napaporn
authors.initials: N
authors.lastname: Youngvises
authors.forename: Thanatcha
authors.initials: T
authors.lastname: Chaida
authors.forename: Supada
authors.initials: S
authors.lastname: Khonyoung
authors.forename: Nattawan
authors.initials: N
authors.lastname: Kuppithayanant
authors.forename: Warawut
authors.initials: W
authors.lastname: Tiyapongpattana
authors.forename: Arunporn
authors.initials: A
authors.lastname: Itharat
authors.forename: Jaroon
authors.initials: J
authors.lastname: Jakmunee
mesh_terms: D000779:Anesthetics, Local / Q000032:analysis*; D002138:Calibration; D056148:Chromatography, Reverse-Phase / Q000295:instrumentation / Q000379:methods*; D045424:Complex Mixtures / Q000737:chemistry; D003358:Cosmetics / Q000737:chemistry*; D055772:Green Chemistry Technology; D006633:Histamine Antagonists / Q000032:analysis*; D006801:Humans; D057230:Limit of Detection; D008823:Micelles; D010226:Parabens / Q000032:analysis*; D012967:Sodium Dodecyl Sulfate
chemical_list: D000779:Anesthetics, Local; D045424:Complex Mixtures; D003358:Cosmetics; D006633:Histamine Antagonists; D008823:Micelles; D010226:Parabens; D012967:Sodium Dodecyl Sulfate