title: Possible relation between the NOS3 gene GLU298ASP polymorphism and bladder cancer in Turkey.
abstract: Endothelial nitric oxide synthase (eNOS), encoded by the NOS3 gene, has been suggested to play an important role in uncontrolled cell growth in several cancer types. The objective of this study was to evaluate the role of the NOS3 Glu298Asp polymorphism in bladder cancer susceptibility in a Turkish population. We determined the genotypes of 66 bladder cancer cases and 88 healthy controls. Genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism analysis. A significant association for NOS3 Glu298Asp heterozygotes genotypes and T allely were found between healthy controls and bladder cancer, respectively (p<0.001: p=0.002). There were no significant associations between any genotypes and the stage, grade, and histological type of bladder cancer. Our study suggested an increased risk role of NOS3 GT genotype in bladder cancer susceptibility in our Turkish population.
authors.affiliation: Haydarpasa Numune Training Hospital, Urology Department, Istanbul, Turkey.
authors.forename: Levent
authors.initials: L
authors.lastname: Verim
authors.forename: Bahar
authors.initials: B
authors.lastname: Toptas
authors.forename: Nazli Ezgi
authors.initials: NE
authors.lastname: Ozkan
authors.forename: Canan
authors.initials: C
authors.lastname: Cacina
authors.forename: Saime
authors.initials: S
authors.lastname: Turan
authors.forename: Gurbet
authors.initials: G
authors.lastname: Korkmaz
authors.forename: Ilhan
authors.initials: I
authors.lastname: Yaylim
mesh_terms: D000483:Alleles; D049109:Cell Proliferation; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D052250:Nitric Oxide Synthase Type III / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D020033:Protein Isoforms / Q000235:genetics; D014421:Turkey; D001749:Urinary Bladder Neoplasms / Q000235:genetics*
chemical_list: D020033:Protein Isoforms; D009569:Nitric Oxide; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III