title: RNA-seq identified a super-long intergenic transcript functioning in adipogenesis.
abstract: RNA transcripts are generally classified into polyA-plus and polyA-minus subgroups due to the presence or absence of a polyA tail at the 3' end. Even though a number of physiologically and pathologically important polyA-minus RNAs have been recently identified, a systematic analysis of the expression and function of these transcripts in adipogenesis is still elusive. To study the potential function of the polyA-minus RNAs in adipogenesis, a dynamic expressional profiling was performed in the induced differentiation of 3T3-L1 cells. In addition to identifying thousands of novel intergenic transcripts, differentiation-synchronized expression was characterized for many of them. Among these, several large intergenic transcripts were found to be upregulated by more than 19-fold during differentiation. Further study demonstrated a fat tissue-specific expression pattern for these regions and identified an adipogenesis-associated long non-coding RNA. Collectively, these lines of evidence contribute to the characterization of a super-long intergenic transcript functioning in adipogenesis.
authors.affiliation: Laboratory of Nucleic Acid Technology and Laboratory of State Key Laboratory of Natural and Biomimetic Drugs, Institute of Molecular Medicine, School of Pharmaceutical Sciences, Peking University, Beijing, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yi
authors.forename: Feng
authors.initials: F
authors.lastname: Yang
authors.forename: Xiaoqiao
authors.initials: X
authors.lastname: Liu
authors.forename: Hongbo
authors.initials: H
authors.lastname: Chen
authors.forename: Ting
authors.initials: T
authors.lastname: Ji
authors.forename: Lixiang
authors.initials: L
authors.lastname: Jiang
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Wang
authors.forename: Zhangping
authors.initials: Z
authors.lastname: Yang
authors.forename: Li-He
authors.initials: LH
authors.lastname: Zhang
authors.forename: Xianfeng
authors.initials: X
authors.lastname: Ding
authors.forename: Zicai
authors.initials: Z
authors.lastname: Liang
authors.forename: Quan
authors.initials: Q
authors.lastname: Du
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism*; D050156:Adipogenesis / Q000235:genetics*; D000818:Animals; D020869:Gene Expression Profiling; D006801:Humans; D051379:Mice; D009928:Organ Specificity; D011061:Poly A / Q000235:genetics; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017423:Sequence Analysis, RNA; D014158:Transcription, Genetic; D015854:Up-Regulation
chemical_list: D062085:RNA, Long Noncoding; D012333:RNA, Messenger; D011061:Poly A
keywords: adipogenesis; high-throughput sequencing; large intergenic non-coding RNA; obesity; polyA-minus RNA