title: [Study on tissue cultivation of adventitious roots of Pseudostellaria heterophylla].
abstract: OBJECTIVE
  To systematically optimize the cultivation conditions of adventitious roots of Pseudostellaria heterophylla.
  
  
  METHODS
  Tissue cultivation technology and ultraviolet spectrophotometry were adopted to observe the effect of inoculum volume, sucrose concentration, inorganic salt concentration, number of cultivation days, gradual scale-up cultivation and bubble different angles of bioreactor on the growth of adventitious roots of P. heterophylla, and determine the content of constituents such as saponin, polysaccharide and amino acid.
  
  
  RESULTS
  The propagation multiple of adventitious roots reached the maximum when the inoculum was 6 g in a 1 L culture shake flask. With the increase in sucrose concentration, the dry weight propagation multiples of adventitious roots followed an up and down trend. The inorganic salt concentration in a cultivation dish had a greater effect on the growth of adventitious roots, particularly 3/4 MS was the most favorable for the growth of adventitious roots. The growth curve of P. heterophylla was "S", with the biomass reaching the maximum at the 28th day.
  
  
  CONCLUSIONS
  The inoculum volume, sucrose concentration, inorganic salt concentration, gradual scale-up cultivation and angles of bubble bioreactor had a significant effect on the growth of adventitious roots of P. heterophylla. The contents of saponin and amino acid in adventitious roots were higher than that in cultivated P. heterophylla, whereas the polysaccharide content were lower than that in cultivated P. heterophylla.
authors.affiliation: Tongren Polytechnic College, Tongren 554300, China.
authors.forename: Yu-Yong
authors.initials: YY
authors.lastname: Liang
authors.forename: Shuang-Shuang
authors.initials: SS
authors.lastname: Yin
authors.forename: Bei-Mei
authors.initials: BM
authors.lastname: Zuo
authors.forename: Wen-Yuan
authors.initials: WY
authors.lastname: Gao
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D018533:Biomass; D019149:Bioreactors; D029748:Caryophyllaceae / Q000254:growth & development* / Q000378:metabolism; D018517:Plant Roots / Q000254:growth & development* / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism; D012503:Saponins / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors; D046509:Tissue Culture Techniques / Q000295:instrumentation / Q000379:methods*
chemical_list: D000596:Amino Acids; D011134:Polysaccharides; D012503:Saponins