title: Fermentanomics informed amino acid supplementation of an antibody producing mammalian cell culture.
abstract: Fermentanomics, or a global understanding of a culture state on the molecular level empowered by advanced techniques like NMR, was employed to show that a model hybridoma culture supplied with glutamine and glucose depletes aspartate, cysteine, methionine, tryptophan, and tyrosine during antibody production. Supplementation with these amino acids prevents depletion and improves culture performance. Furthermore, no significant changes were observed in the distribution of glycans attached to the IgG3 in cultures supplemented with specific amino acids, arguing that this strategy can be implemented without fear of impact on important product quality attributes. In summary, a targeted strategy of quantifying media components and designing a supplementation strategy can improve bioprocess cell cultures when enpowered by fermentanomics tools.
authors.affiliation: Div. of Monoclonal Antibodies, CDER, FDA, Silver Spring, MD, USA.
authors.forename: Erik K
authors.initials: EK
authors.lastname: Read
authors.forename: Scott A
authors.initials: SA
authors.lastname: Bradley
authors.forename: Tim A
authors.initials: TA
authors.lastname: Smitka
authors.forename: Cyrus D
authors.initials: CD
authors.lastname: Agarabi
authors.forename: Scott C
authors.initials: SC
authors.lastname: Lute
authors.forename: Kurt A
authors.initials: KA
authors.lastname: Brorson
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D000818:Animals; D000906:Antibodies / Q000378:metabolism*; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D005285:Fermentation; D006825:Hybridomas / Q000378:metabolism; D051379:Mice; D011134:Polysaccharides / Q000378:metabolism
chemical_list: D000596:Amino Acids; D000906:Antibodies; D003470:Culture Media; D011134:Polysaccharides