title: Association of prostate cancer susceptibility variant (MSMB) rs10993994 with risk of spermatogenic failure.
abstract: β-Microseminoprotein (MSMB) is one of the most abundant proteins in human seminal plasma. It has been identified that MSMB increased significantly in oligoasthenoteratozoospermic patients compared with fertile controls. We hypothesized that the functional polymorphism (rs10993994) of MSMB gene could be a risk factor for spermatogenic failure. For this study, 338 patients with idiopathic oligozoospermia or azoospermia and 382 fertile controls were recruited from an infertility clinic. Semen analysis was performed by computer-assisted semen analysis system. The functional polymorphism of MSMB gene was genotyped using TaqMan method. Sixty three seminal plasma samples were used to test the expression of MSMB by enzyme-linked immunosorbent assay (ELISA). The TT genotype and T allele were associated with an increased risk of idiopathic infertility with azoospermia (TT genotype: OR, 1.75; 95% CI, 1.03-2.95; T allele: OR, 1.34; 95% CI, 1.03-1.75). However, no differences were found in risk for the TT genotype or T allele among men with oligozoospermia. In addition, idiopathic infertile males have significantly higher MSMB expression levels than fertile controls. We present the first epidemiologic evidence supporting the involvement of common genetic polymorphism in MSMB gene in spermatogenic failure. These results suggest that men carrying the variant have an increased risk of spermatogenic failure associated with male infertility. Further studies are needed to confirm the roles of the polymorphism in idiopathic azoospermia and investigate the biological mechanism of elevated MSMB expression in infertile males.
authors.affiliation: State Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, Nanjing 210029, China. wwu@njmu.edu.cn
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.forename: Jing
authors.initials: J
authors.lastname: Lu
authors.forename: Beilei
authors.initials: B
authors.lastname: Yuan
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Qin
authors.forename: Minjian
authors.initials: M
authors.lastname: Chen
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Niu
authors.forename: Bin
authors.initials: B
authors.lastname: Xu
authors.forename: Chuncheng
authors.initials: C
authors.lastname: Lu
authors.forename: Yankai
authors.initials: Y
authors.lastname: Xia
authors.forename: Daozhen
authors.initials: D
authors.lastname: Chen
authors.forename: Jiahao
authors.initials: J
authors.lastname: Sha
authors.forename: Xinru
authors.initials: X
authors.lastname: Wang
mesh_terms: D000328:Adult; D000483:Alleles; D044466:Asian People / Q000235:genetics; D053713:Azoospermia / Q000208:ethnology / Q000235:genetics*; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D015972:Gene Expression Regulation, Neoplastic; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease / Q000208:ethnology; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008297:Male; D016017:Odds Ratio; D009845:Oligospermia / Q000235:genetics; D011471:Prostatic Neoplasms / Q000235:genetics*; D029584:Prostatic Secretory Proteins / Q000235:genetics*; D012307:Risk Factors; D055101:Semen Analysis
chemical_list: D029584:Prostatic Secretory Proteins; C043076:beta-microseminoprotein