title: On the metabolism of the mycotoxin deoxynivalenol in the isolated perfused rat liver.
abstract: An isolated rat liver was perfused with deoxynivalenol (DON) at a dose of 3 mg in a recirculating perfusion system. To identify glucuronide conjugates equal amounts of bile samples, perfusate and liver homogenates were incubated with and without (control) a β-glucuronidase preparation and analyzed by thin layer chromatography and capillary gas liquid chromatography - chemical ionization mass spectrometry. A total of 40.4% of the administered dose of DON was found to be conjugated with glucuronic acid (perfusate 20.4%, bile 19.2%, liver 0.8%), while only 1.3% of the parent DON (perfusate 1.1%, bile 0.2%) was detected. The cleavage of DON-glucuronide was demonstrated by incubating DON-glucuronide containing bile samples with intestine contents under anaerobic conditions.
authors.affiliation: Institute for Medical Microbiology, Infectious and Epidemic Diseases Faculty of Veterinary Medicine, University of Munich, Veterinärstrasse 13, D-8000, Munich 22, FRG.
authors.forename: M
authors.initials: M
authors.lastname: Gareis
authors.forename: J
authors.initials: J
authors.lastname: Bauer
authors.forename: B
authors.initials: B
authors.lastname: Gedek