title: [Identification of EST-SSRs in Taxus cuspidata based on high-throughput sequencing].
abstract: OBJECTIVE
  Taxus species are highly valued for the production of taxol. Based on high-throughput sequenceing, EST-SSRs were explored and studied in the transcriptome of Taxus cuspidata.
  
  
  METHODS
  T cuspidata leaf cDNA was extracted and sequenced by 454 GS FLX Titanium. High-quality sequences were assembled using Newbler Assembler Software, which produced unique sequences. SSRs motif was explored using simple sequence repeat identification tool (Perl Script). Primers were designed using PRIMER3.
  
  
  RESULTS
  A total of 81 148 high-quality reads from the needles of T. cuspidata were produced using the Roche GS FLX Titanium system. A total of 20 557 unique sequences were obtained. There were 753 simple sequence repeat motifs identified. Primers of PCR were obtained for 519 EST-SSRs, randomly selected cloning sequencing revealed that 87.5% of ESTs were the same as the results of Sanger sequencing.
  
  
  CONCLUSIONS
  The results provide the first EST-SSRs collection in Taxus and are essential for future efforts of gene discovery, functional genomics, and genome annotation in related species.
authors.affiliation: College of Pharmacy, Guilin Medical University, Guilin 541004, China. qwu9516@gmail.com
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.forename: Xiao-Qun
authors.initials: XQ
authors.lastname: Duan
authors.forename: Xu
authors.initials: X
authors.lastname: Chen
authors.forename: Pei-Gen
authors.initials: PG
authors.lastname: Xiao
mesh_terms: D017931:DNA Primers / Q000235:genetics; D020224:Expressed Sequence Tags; D020869:Gene Expression Profiling / Q000379:methods; D018506:Gene Expression Regulation, Plant; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D018895:Microsatellite Repeats / Q000235:genetics*; D020946:Taxus / Q000235:genetics*; D059467:Transcriptome / Q000235:genetics
chemical_list: D017931:DNA Primers