title: Reversible immobilization of invertase on Cu-chelated polyvinylimidazole-grafted iron oxide nanoparticles.
abstract: Polyvinylimidazole (PVI)-grafted iron oxide nanoparticles (PVIgMNP) were prepared by grafting of telomere of PVI on the iron oxide nanoparticles. Different metal ions (Cu(2+), Zn(2+), Cr(2+), Ni(2+)) ions were chelated on polyvinylimidazole-grafted iron oxide nanoparticles, and then the metal-chelated magnetic particles were used in the adsorption of invertase. The maximum invertase immobilization capacity of the PVIgMNP-Cu(2+) beads was observed to be 142.856 mg/g (invertase/PVIgMNP) at pH 5.0. The values of the maximum reaction rate (V max) and Michaelis-Menten constant (Km) were determined for the free and immobilized enzymes. The enzyme adsorption-desorption studies, pH effect on the adsorption efficiency, affinity of different metal ions, the kinetic parameters and storage stability of free and immobilized enzymes were evaluated.
authors.affiliation: Department of Chemistry, Faculty of Arts and Sciences, Fatih University, Buyukcekmece Kampusu, 34500, B.Cekmece, Istanbul, Turkey.
authors.forename: Kerem
authors.initials: K
authors.lastname: Uzun
authors.forename: Emre
authors.initials: E
authors.lastname: Çevik
authors.forename: Mehmet
authors.initials: M
authors.lastname: Şenel
authors.forename: Abdülhadi
authors.initials: A
authors.lastname: Baykal
mesh_terms: D000327:Adsorption; D002614:Chelating Agents / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000737:chemistry*; D053768:Metal Nanoparticles; D046529:Microscopy, Electron, Transmission; D011145:Polyvinyls / Q000737:chemistry*; D013696:Temperature; D043324:beta-Fructofuranosidase / Q000737:chemistry*
chemical_list: D002614:Chelating Agents; D004800:Enzymes, Immobilized; D005290:Ferric Compounds; D007093:Imidazoles; D011145:Polyvinyls; C000499:ferric oxide; C072974:polyvinylimidazole; D003300:Copper; D043324:beta-Fructofuranosidase