title: [Cloning of Blastocystis sp subtype 3 small-subunit ribosomal DNA].
abstract: OBJECTIVE
  Different sub-types or genetic variations of Blastocystis sp. are thought to play a role in the differential symptoms caused by the parasite or asymptomatic cases. In this study, it was aimed to clone a fragment of SSUrDNA gene of Blastocystis from a patient in order to define its phylogenetic subtype.
  
  
  METHODS
  In this study, DNA isolation from the stool of a Blastocystis infected patient was performed. Blastocystis specific primers were used to amplify a SSUrDNA genomic fragment. The amplified DNA fragment was cloned into a plasmid and sequenced using plasmid specific primers. The obtained DNA sequence was analyzed using BLAST and a phylogenetic tree was constructed using the software MEGA.
  
  
  RESULTS
  It was found that the Blastocystis isolate in our study is subtype 3.
  
  
  CONCLUSIONS
  Cloning and sequencing of the target genomic region is suggested for phylogenetic analysis studies.
authors.affiliation: Erciyes Üniversitesi Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, Kayseri, Türkiye.
authors.forename: Çağrı
authors.initials: Ç
authors.lastname: Şakalar
authors.forename: Yunus
authors.initials: Y
authors.lastname: Uyar
authors.forename: Mehmet Ali
authors.initials: MA
authors.lastname: Yürürdurmaz
authors.forename: Sadık
authors.initials: S
authors.lastname: Tokar
authors.forename: Huzeyfe
authors.initials: H
authors.lastname: Yeşilkaya
authors.forename: Esra
authors.initials: E
authors.lastname: Gürbüz
authors.forename: Salih
authors.initials: S
authors.lastname: Kuk
authors.forename: Süleyman
authors.initials: S
authors.lastname: Yazar
mesh_terms: D016844:Blastocystis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D016776:Blastocystis Infections / Q000469:parasitology*; D003001:Cloning, Molecular; D017931:DNA Primers; D016054:DNA, Protozoan / Q000235:genetics* / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000235:genetics* / Q000302:isolation & purification; D005243:Feces / Q000469:parasitology; D014644:Genetic Variation; D006801:Humans; D010802:Phylogeny; D054679:Ribosome Subunits, Small / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D016054:DNA, Protozoan; D004275:DNA, Ribosomal