title: Tetrapisispora taiwanensis sp. nov. and Tetrapisispora pingtungensis sp. nov., two ascosporogenous yeast species isolated from soil.
abstract: Two novel yeast species, Tetrapisispora taiwanensis sp. nov. and Tetrapisispora pingtungensis sp. nov., belonging to the Saccharomycetaceae within the Saccharomycetales, are proposed to accommodate six strains isolated from samples of Taiwanese forest soil between 2005 and 2010. Sequence analysis of the internal transcribed spacer (ITS) and the D1/D2 domains of the large-subunit (LSU) rRNA gene revealed that the two species are phylogenetically closely related to species of the genus Tetrapisispora. Moreover, the assimilation spectrum of carbon and nitrogen sources and morphological characteristics are very similar to those of other Tetrapisispora species. The molecular, morphological and physiological characteristics described above indicate that these two species should be classified as members of the genus Tetrapisispora. The two species can be differentiated from each other and from other Tetrapisispora species based on their LSU D1/D2 rRNA gene and ITS sequences. Thus, the two species could be regarded as novel species of the genus Tetrapisispora, and the names Tetrapisispora taiwanensis sp. nov. (type strain SJ6S04(T) =CBS 10586(T) =NBRC 102652(T) =BCRC 23090(T)) and Tetrapisispora pingtungensis sp. nov. (type strain NC2S06(T) =CBS 12780(T) =BCRC 23409(T)) are proposed. The type strains of the two species were isolated from forest soil in Leinhuatsu Park, Nantou, in 2006 and from Hungchun, Pingtung, in 2009, respectively.
authors.affiliation: Department of Hospitality Management, Chu Hwa University of Medical Technology, 91 Wenhua 1st Street, Zender, Tainan, 717 Taiwan.
authors.forename: Shan-Fu
authors.initials: SF
authors.lastname: Chen
authors.affiliation: Department of Applied Science, National Hsinchu University of Education, 521 Nanda Road, Hsinchu, 300 Taiwan.
authors.forename: Shih Feng
authors.initials: SF
authors.lastname: Lo
authors.affiliation: Department of Applied Science, National Hsinchu University of Education, 521 Nanda Road, Hsinchu, 300 Taiwan.
authors.forename: Chin-Feng
authors.initials: CF
authors.lastname: Chang
authors.affiliation: Department of Applied Science, National Hsinchu University of Education, 521 Nanda Road, Hsinchu, 300 Taiwan.
authors.forename: Ching-Fu
authors.initials: CF
authors.lastname: Lee
mesh_terms: D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010802:Phylogeny; D004718:Saccharomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013624:Taiwan; D014197:Trees / Q000382:microbiology
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer