title: Fabrication and bioproperties of raspberry-type hybrid nanoparticles of Au-thioethyl pendant ligand@chitosan.
abstract: Synthesis of nanoparticles with desired size/morphology has enormous importance, especially in the compelling field of nanotechnology. In this case, a novel kind of raspberry-type hybrid nanoparticles was prepared by hybridization of chitosan (CS) with thioethyl pendant ligand (TPL) modified Au nanoparticles (Au-TPL@CS NPs). Such method was based on ionic gelation using sodium tripolyphosphate as a counterion. The blood compatibility of Au-TPL@CS NPs was characterized by coagulation tests, plasma recalcification time, hemolysis assay, morphological changes of red blood cells (RBCs) and complement activation in vitro. The results showed that Au-TPL@CS NPs exhibited good blood compatibility. The possible underlying mechanism was also present. Finally, the direct electron transfer reactivity of the Hemoglobin/Au-TPL@CS NPs/multi-walled carbon nanotubes/glassy carbon electrode was investigated with cyclic voltammetry measurements. The biosensor exhibited a good electrocatalytic activity to the reduction of H2O2. Such new type of Au-TPL@CS NPs provides a promising platform of biological system for early illness detection and treatment in future.
authors.affiliation: School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Huang
authors.forename: Fengyu
authors.initials: F
authors.lastname: Tong
authors.forename: Yeting
authors.initials: Y
authors.lastname: Chen
authors.forename: Qinshu
authors.initials: Q
authors.lastname: Zhu
authors.forename: Lei
authors.initials: L
authors.lastname: Xing
authors.forename: Mengqi
authors.initials: M
authors.lastname: Lv
authors.forename: Chun
authors.initials: C
authors.lastname: Mao
authors.forename: Zuhong
authors.initials: Z
authors.lastname: Lu
authors.forename: Jian
authors.initials: J
authors.lastname: Shen
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology; D001777:Blood Coagulation; D048271:Chitosan / Q000138:chemical synthesis* / Q000737:chemistry; D003167:Complement Activation / Q000187:drug effects; D055664:Electrochemical Techniques; D004566:Electrodes; D004583:Electrons; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects; D006046:Gold / Q000737:chemistry*; D006461:Hemolysis / Q000187:drug effects; D008024:Ligands; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D055616:Microtechnology / Q000379:methods*; D037742:Nanotubes, Carbon; D011817:Rabbits; D013457:Sulfur Compounds / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D000925:Anticoagulants; D008024:Ligands; D037742:Nanotubes, Carbon; D013457:Sulfur Compounds; D006046:Gold; D048271:Chitosan