title: Chromatographic determination of aliphatic aldehydes in human serum after pre-column derivatization using 2,2'-furil, a novel fluorogenic reagent.
abstract: A novel, highly sensitive and selective fluorimetric liquid chromatographic method for simultaneous determination of medium chain aliphatic aldehydes was developed. The method was based on the derivatization of aliphatic aldehydes with 1,2-di(2-furyl)-1,2-ethanedione (2,2'-furil), a novel fluorogenic reagent, to form highly fluorescent difurylimidazole derivatives. The fluorescence derivatives were separated in less than 20min on a reversed-phase ODS column using an isocratic elution with a mixture of methanol-water (80:20, v/v%). The detection limits were from 0.19 to 0.50nM (1-10fmol/injection) at a signal-to-noise ratio (S/N) of 3. This method was successfully applied for monitoring of aliphatic aldehydes in healthy human sera by a simple pretreatment procedure without interferences from serum constituents.
authors.affiliation: Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt.
authors.forename: Marwa
authors.initials: M
authors.lastname: Fathy Bakr Ali
authors.forename: Naoya
authors.initials: N
authors.lastname: Kishikawa
authors.forename: Kaname
authors.initials: K
authors.lastname: Ohyama
authors.forename: Horria
authors.initials: H
authors.lastname: Abdel-Mageed Mohamed
authors.forename: Hanaa
authors.initials: H
authors.lastname: Mohamed Abdel-Wadood
authors.forename: Ashraf
authors.initials: A
authors.lastname: Mohamed Mohamed
authors.forename: Naotaka
authors.initials: N
authors.lastname: Kuroda
mesh_terms: D000447:Aldehydes / Q000097:blood* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D005663:Furans / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D015203:Reproducibility of Results; D059629:Signal-To-Noise Ratio
chemical_list: D000447:Aldehydes; D005456:Fluorescent Dyes; D005663:Furans; C511839:alpha-furil
keywords: 1,2-Di(2-furyl)-1,2-ethanedione (2,2′-furil); Aliphatic aldehydes (lipid peroxidation); Difurylimidazole derivatives; Pre-column fluorogenic derivatization; Serum analysis