title: Development and evaluation of a calibrator material for nucleic acid-based assays for diagnosing aspergillosis.
abstract: Twelve laboratories evaluated candidate material for an Aspergillus DNA calibrator. The DNA material was quantified using limiting-dilution analysis; the mean concentration was determined to be 1.73 × 10(10) units/ml. The calibrator can be used to standardize aspergillosis diagnostic assays which detect and/or quantify nucleic acid.
authors.affiliation: Emory University School of Medicine, Atlanta, Georgia, USA. gmlyon@emory.edu
authors.forename: G Marshall
authors.initials: GM
authors.lastname: Lyon
authors.forename: Deborah
authors.initials: D
authors.lastname: Abdul-Ali
authors.forename: Juergen
authors.initials: J
authors.lastname: Loeffler
authors.forename: P Lewis
authors.initials: PL
authors.lastname: White
authors.forename: Brian
authors.initials: B
authors.lastname: Wickes
authors.forename: Monica L
authors.initials: ML
authors.lastname: Herrera
authors.forename: Barbara D
authors.initials: BD
authors.lastname: Alexander
authors.forename: Lindsey R
authors.initials: LR
authors.lastname: Baden
authors.forename: Cornelius
authors.initials: C
authors.lastname: Clancy
authors.forename: David
authors.initials: D
authors.lastname: Denning
authors.forename: M Hong
authors.initials: MH
authors.lastname: Nguyen
authors.forename: Michele
authors.initials: M
authors.lastname: Sugrue
authors.forename: L Joseph
authors.initials: LJ
authors.lastname: Wheat
authors.forename: John R
authors.initials: JR
authors.lastname: Wingard
authors.forename: J Peter
authors.initials: JP
authors.lastname: Donnelly
authors.forename: Rosemary
authors.initials: R
authors.lastname: Barnes
authors.forename: Thomas F
authors.initials: TF
authors.lastname: Patterson
authors.forename: Angela M
authors.initials: AM
authors.lastname: Caliendo
mesh_terms: D001228:Aspergillosis / Q000175:diagnosis*; D001230:Aspergillus / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics*; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods / Q000592:standards*; D025202:Molecular Diagnostic Techniques / Q000379:methods / Q000592:standards*; D012015:Reference Standards
chemical_list: D004271:DNA, Fungal