title: Tumor suppressive miR-509-5p contributes to cell migration, proliferation and antiapoptosis in renal cell carcinoma.
abstract: OBJECTIVE
  The aim of this study was to determine the expression and function of miR-509-5p in renal cell carcinoma (RCC).
  
  
  METHODS
  In this research, we have conducted quantitative real-time polymerase chain reaction (qRT-PCR) assay to determine the expression level of miR-509-5p in tissues and plasma from renal cell carcinoma patients. We preformed in vitro migration scratch assay, flow cytometry analysis and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay to determine the exact function of miR-509-5p.
  
  
  RESULTS
  We evaluated the expression level of miR-509-5p in RCC tissues and paired adjacent normal tissues from 42 patients and found that miR-509-5p expression in 42 RCC specimens was significantly down-regulated compared to that in adjacent normal tissue. Furthermore, the level of miR-509-5p in RCC patients' plasma was significantly lower than that in control plasma. In addition, the overexpression of miR-509-5p suppressed the proliferation of RCC cell (786-0), induced cell apoptosis and inhibited cell migration in vitro.
  
  
  CONCLUSIONS
  In this study, we have shown that miR-509-5p played an important role in RCC by inhibiting cell proliferation and migration and by promoting cell apoptosis. In addition, miR-509-5p expression was significantly lower in RCC patient plasma compared to that in normal individuals.
authors.affiliation: Department of Urology, Zhongnan Hospital, Wuhan University, 169 Donghu Road, Wuhan, China, zhangweibin1969@163.com.
authors.forename: W-B
authors.initials: WB
authors.lastname: Zhang
authors.forename: Z-Q
authors.initials: ZQ
authors.lastname: Pan
authors.forename: Q-S
authors.initials: QS
authors.lastname: Yang
authors.forename: X-M
authors.initials: XM
authors.lastname: Zheng
mesh_terms: D000293:Adolescent; D000328:Adult; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D002292:Carcinoma, Renal Cell / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002648:Child; D002675:Child, Preschool; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007223:Infant; D007680:Kidney Neoplasms / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D008297:Male; D035683:MicroRNAs / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D014162:Transfection; D055815:Young Adult
chemical_list: D014408:Biomarkers, Tumor; C571982:MIRN509 microRNA, human; D035683:MicroRNAs