title: Highly water-soluble BODIPY-based fluorescent probe for sensitive and selective detection of nitric oxide in living cells.
abstract: A highly water-soluble BODIPY dye bearing electron-rich o-diaminophenyl groups at 2,6-positions was prepared as a highly sensitive and selective fluorescent probe for detection of nitric oxide (NO) in living cells. The fluorescent probe displays an extremely weak fluorescence with fluorescence quantum yield of 0.001 in 10 mM phosphate buffer (pH 7.0) in the absence of NO as two electron-rich o-diaminophenyl groups at 2,6-positions significantly quench the fluorescence of the BODIPY dye via photoinduced electron transfer mechanism. The presence of NO in cells enhances the dye fluorescence dramatically. The fluorescent probe demonstrates excellent water solubility, membrane permeability, and compatibility with living cells for sensitive detection of NO.
authors.affiliation: Department of Chemistry, Michigan Technological University, Houghton, Michigan 49931, United States.
authors.forename: Giri K
authors.initials: GK
authors.lastname: Vegesna
authors.forename: Srinivas R
authors.initials: SR
authors.lastname: Sripathi
authors.forename: Jingtuo
authors.initials: J
authors.lastname: Zhang
authors.forename: Shilei
authors.initials: S
authors.lastname: Zhu
authors.forename: Weilue
authors.initials: W
authors.lastname: He
authors.forename: Fen-Tair
authors.initials: FT
authors.lastname: Luo
authors.forename: Wan Jin
authors.initials: WJ
authors.lastname: Jahng
authors.forename: Megan
authors.initials: M
authors.lastname: Frost
authors.forename: Haiying
authors.initials: H
authors.lastname: Liu
mesh_terms: D000818:Animals; D001896:Boron Compounds / Q000737:chemistry*; D002460:Cell Line; D005456:Fluorescent Dyes / Q000737:chemistry*; D057230:Limit of Detection; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D009569:Nitric Oxide / Q000032:analysis*; D012995:Solubility; D014867:Water / Q000737:chemistry*
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D014867:Water; D009569:Nitric Oxide