title: In vitro evaluation of combined sulfated silk fibroin scaffolds for vascular cell growth.
abstract: A combined sulfated silk fibroin scaffold is fabricated by modifying a knitted silk scaffold with sulfated silk fibroin sponges. In vitro hemocompatibility evaluation reveals that the combined sulfated silk fibroin scaffolds reduce platelet adhesion and activation, and prolong the activated partial thromboplastin time (APTT), thrombin time (TT), and prothrombin time (PT). The response of porcine endothelial cells (ECs) and smooth muscle cells (SMCs) on the scaffolds is studied to evaluate the cytocompatibility of the scaffolds. Vascular cells are seeded on the scaffolds and cultured for 2 weeks. The scaffolds demonstrate enhanced EC adhesion, proliferation, and maintenance of cellular functions. Moreover, the scaffolds inhibit SMC proliferation and induce expression of contractile SMC marker genes.
authors.affiliation: Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing 100191, People's Republic of China. haifengliu@buaa.edu.cn
authors.forename: Haifeng
authors.initials: H
authors.lastname: Liu
authors.forename: Xili
authors.initials: X
authors.lastname: Ding
authors.forename: Yanxue
authors.initials: Y
authors.lastname: Bi
authors.forename: Xianghui
authors.initials: X
authors.lastname: Gong
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Li
authors.forename: Gang
authors.initials: G
authors.lastname: Zhou
authors.forename: Yubo
authors.initials: Y
authors.lastname: Fan
mesh_terms: D000818:Animals; D001808:Blood Vessels / Q000166:cytology* / Q000187:drug effects*; D002452:Cell Count; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D004247:DNA / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D005349:Fibroins / Q000494:pharmacology* / Q000648:ultrastructure; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008422:Materials Testing; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D010314:Partial Thromboplastin Time; D010973:Platelet Adhesiveness / Q000187:drug effects; D011517:Prothrombin Time; D017550:Spectroscopy, Fourier Transform Infrared; D013431:Sulfates / Q000494:pharmacology*; D034421:Sus scrofa; D013918:Thrombin Time; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D013431:Sulfates; D004247:DNA; D005349:Fibroins