title: Genotyping of Toxoplasma gondii isolates from wild boars in Peninsular Malaysia.
abstract: Toxoplasma gondii is a parasitic protozoan that infects nearly one-third of the world population. The present study was done to isolate and genotype T. gondii from wild boar from forests of Pahang, Malaysia. A total of 30 wild boars' blood, heads and hearts were obtained for this study and 30 (100.0%) were found to be seropositive when assayed with modified agglutination test (MAT ≥ 6). The positive samples were inoculated into mice and T. gondii was only isolated from samples that had strong seropositivity (MAT ≥ 1:24).The isolates were subjected to PCR-RFLP analysis and all the Peninsular Malaysia isolates of T. gondii are of clonal type I.
authors.affiliation: Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Penang, Malaysia.
authors.forename: Vignesh R
authors.initials: VR
authors.lastname: Puvanesuaran
authors.forename: Rahmah
authors.initials: R
authors.lastname: Noordin
authors.forename: Venugopal
authors.initials: V
authors.lastname: Balakrishnan
mesh_terms: D000372:Agglutination Tests; D000818:Animals; D005819:Genetic Markers; D060005:Genotyping Techniques; D008296:Malaysia; D051379:Mice; D012150:Polymorphism, Restriction Fragment Length; D034421:Sus scrofa / Q000469:parasitology*; D014122:Toxoplasma / Q000235:genetics* / Q000302:isolation & purification*; D014124:Toxoplasmosis, Animal / Q000469:parasitology*
chemical_list: D005819:Genetic Markers