title: Yeasts from autochthonal cheese starters: technological and functional properties.
abstract: OBJECTIVE
  The aim of this work was to identify 20 yeasts isolated from autochthonal cheese starters and evaluate their technological and functional properties.
  
  
  RESULTS
  The capacities of the yeasts to grow at different temperatures, pH, NaCl and lactic acid concentrations as well as the proteolytic and lipolytic activities were studied. Moreover, survival to simulated gastrointestinal digestion, hydrophobicity, antimicrobial activity against pathogens and auto- and co-aggregation abilities were evaluated. The sequentiation of a fragment from the 26S rDNA gene indicated that Kluyveromyces marxianus was the predominant species, followed by Saccharomyces cerevisiae, Clavispora lusitaniae, Kluyveromyces lactis and Galactomyces geotrichum. RAPD with primer M13 allowed a good differentiation among strains from the same species. All strains normally grew at pH 4.7-5.5 and temperatures between 15 and 35°C. Most of them tolerated 10% NaCl and 3% lactic acid. Some strains showed proteolytic (eight isolates) and/or lipolytic (four isolates) capacities. All strains evidenced high gastrointestinal resistance, moderate hydrophobicity, intermediate auto-aggregation and variable co-aggregation abilities. No strains inhibited the growth of the pathogens assayed.
  
  
  CONCLUSIONS
  Some strains from dairy sources showed interesting functional and technological properties.
  
  
  CONCLUSIONS
  This study has been the first contribution to the identification and characterization of yeasts isolated from autochthonal cheese starters in Argentina. Many strains could be proposed as potential candidates to be used as probiotics and/or as co-starters in cheese productions.
authors.affiliation: Instituto de Lactología Industrial (INLAIN, UNL - CONICET), Santa Fe, Argentina.
authors.forename: A
authors.initials: A
authors.lastname: Binetti
authors.forename: M
authors.initials: M
authors.lastname: Carrasco
authors.forename: J
authors.initials: J
authors.lastname: Reinheimer
authors.forename: V
authors.initials: V
authors.lastname: Suárez
mesh_terms: D001118:Argentina; D002611:Cheese / Q000382:microbiology*; D041981:Gastrointestinal Tract / Q000382:microbiology; D007716:Kluyveromyces / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology; D008066:Lipolysis; D050296:Microbial Viability; D019936:Probiotics; D059748:Proteolysis; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology; D012965:Sodium Chloride / Q000494:pharmacology; D013696:Temperature; D015003:Yeasts / Q000254:growth & development / Q000302:isolation & purification / Q000502:physiology*
chemical_list: D012965:Sodium Chloride
keywords: dairy starter yeasts; dairy yeasts; probiotic yeasts; yeast characterization; yeast identification