title: The use of hydrophilic poly(N,N-dimethylacrylamide) for promoting engulfment of magnetic gamma-Fe2O3 nanoparticles by mammalian cells.
abstract: gamma-Fe2O3 nanoparticles obtained by coprecipitation of Fe(II) and Fe(III) chlorides with a base and subsequent oxidation were coated with a shell of hydrophilic biocompatible poly(N,N-dimethylacrylamide) (PDMAAm). Various initiators were attached to the iron oxide surface to enable the use of the "grafting-from" approach for immobilization of PDMAAm. They included 2,2'-azobis(2-methylpropanimidamide) dihydrochloride (AMPA), 2,2'-azobis(N-hydroxy-2-methylpropanimidamide) dihydrochloride (ABHA) and 4-cyano-4-{[1-cyano-3-(N-hydroxycarbamoyl)-1-methylpropyl]azo}pentanoic acid (CCHPA). Engulfment of PDMAAm-coated y-Fe2O3 nanoparticles by murine J774.2 macrophages was investigated. Only some nanoparticles were engulfed by the macrophages. PDMAAm-AMPA-gamma-Fe2O3 and PDMAAm-ABHA-y-Fe2O3 nanoparticles were rapidly engulfed by the cells. In contrast, neat y-Fe2O3 and PDMAAm-CCHPA-gamma-Fe2O3 particles induced formation of transparent vacuoles indicating toxicity of the particles. Thus, PDMAAm-coated AMPA- and ABHA-gamma-Fe2O3 nanoparticles can be recommended as non-toxic labels for mammalian cells.
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovského Sq. 2, 162 06 Prague 6, Czech Republic.
authors.forename: Beata Anna
authors.initials: BA
authors.lastname: Zasońska
authors.forename: Nataliya
authors.initials: N
authors.lastname: Boiko
authors.forename: Daniel
authors.initials: D
authors.lastname: Horák
authors.forename: Olga
authors.initials: O
authors.lastname: Klyuchivska
authors.forename: Hana
authors.initials: H
authors.lastname: Macková
authors.forename: Milan J
authors.initials: MJ
authors.lastname: Benes
authors.forename: Michal
authors.initials: M
authors.lastname: Babic
authors.forename: Miroslava
authors.initials: M
authors.lastname: Trchová
authors.forename: Jirina
authors.initials: J
authors.lastname: Hromádková
authors.forename: Rostyslav
authors.initials: R
authors.lastname: Stoika
mesh_terms: D000178:Acrylamides / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D002460:Cell Line; D005290:Ferric Compounds / Q000494:pharmacology*; D057927:Hydrophobic and Hydrophilic Interactions / Q000187:drug effects*; D008027:Light; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D060328:Magnetic Phenomena; D059346:Magnets; D008322:Mammals / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D058105:Polymerization / Q000187:drug effects; D012542:Scattering, Radiation; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000178:Acrylamides; D005290:Ferric Compounds; C429790:poly(N,N-dimethylacrylamide); C000499:ferric oxide