title: Cytotoxic effects of palladium (II) and platinum (II) complexes with O,O'-dialkyl esters of (S,S)-ethylenediamine-N,N'-di-2-(4-methyl) pentanoic acid on human colon cancer cell lines.
abstract: OBJECTIVE
  As novel therapeutic agents relevant to colon cancer therapy are explored continuously, we tested 4 R2edda-type ligand precursors O,O'-dialkyl esters of (S,S)-ethylenediamine-N,N'-di-2-(4-methyl)pentanoic acid (L1.2HCl-L4.2HCl) and corresponding palladium(II) and platinum(II) complexes against the human colon cancer cell lines CaCo-2, SW480 and HCT116.
  
  
  METHODS
  The effects of the tested compounds on cell viability were determined using MTT colorimetric technique.
  
  
  RESULTS
  Analysis of cancer cell viability showed that all tested ligand precursors, palladium(II) and platinum(II) complexes were cytotoxic on human colon cancer cells in dose-dependent manner. The cytotoxic activity of all palladium(II) and platinum(II) complexes toward selected cancer cells was significantly higher in comparison to cisplatin. Among the tested platinum(II) and palladium(II) complexes the lowest activity was observed for the compounds with the shortest ester chain and the highest activity was noted for palladium(II) complex No.2 with the n-Pr group in ester chain and for platinum(II) complex No.7 with the n-Bu group in ester chain.
  
  
  CONCLUSIONS
  Palladium(II) complex No.2 and platinum(II) complex No.7 seem to be good candidates for future pharmacological evaluation in the field of colon cancer research and treatment.
authors.affiliation: Centre for Molecular Medicine and Stem Cell Research, University of Kragujevac, Kragujevac, Serbia.
authors.forename: V
authors.initials: V
authors.lastname: Volarevic
authors.forename: J M
authors.initials: JM
authors.lastname: Vujic
authors.forename: M
authors.initials: M
authors.lastname: Milovanovic
authors.forename: T
authors.initials: T
authors.lastname: Kanjevac
authors.forename: A
authors.initials: A
authors.lastname: Volarevic
authors.forename: S R
authors.initials: SR
authors.lastname: Trifunovic
authors.forename: N
authors.initials: N
authors.lastname: Arsenijevic
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D018938:Caco-2 Cells; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology; D003110:Colonic Neoplasms / Q000473:pathology*; D004305:Dose-Response Relationship, Drug; D005029:Ethylenediamines / Q000737:chemistry / Q000494:pharmacology*; D045325:HCT116 Cells; D006801:Humans; D015394:Molecular Structure; D009944:Organoplatinum Compounds / Q000737:chemistry / Q000494:pharmacology*; D010165:Palladium / Q000737:chemistry / Q000494:pharmacology*; D010421:Pentanoic Acids / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship
chemical_list: D000970:Antineoplastic Agents; D005029:Ethylenediamines; D009944:Organoplatinum Compounds; D010421:Pentanoic Acids; D010165:Palladium; D002945:Cisplatin