title: Açai (Euterpe oleracea Mart.) feeding attenuates dimethylhydrazine-induced rat colon carcinogenesis.
abstract: This study investigated the protective effect of spray-dried açaí powder (AP) intake on colon carcinogenesis induced by 1,2-dimethylhydrazine (DMH) in male Wistar rats. After 4 weeks of DMH administrations, the groups were fed with standard diet, a diet containing 2.5% or 5.0% AP or a diet containing 0.2% N-acetylcysteine (NAC) for 10 weeks, using aberrant crypt foci (ACF) as the endpoint. Additionally, two groups were fed with standard diet or a diet containing 5.0% AP for 20 weeks, using colon tumors as the endpoint. In ACF assay, a reduction in the number of aberrant crypts (ACs) and ACF (1-3 AC) were observed in the groups fed with 5.0% AP (37% AC and 47% ACF inhibition, p=0.036) and 0.2% NAC (39% AC and 41% ACF inhibition, p=0.042). In tumor assay, a reduction in the number of invasive tumors (p<0.005) and tumor multiplicity (p=0.001) was observed in the group fed with 5.0% AP. Also, a reduction in tumor Ki-67 cell proliferation (p=0.003) and net growth index (p=0.001) was observed in the group fed with 5.0% AP. Therefore the findings of this study indicate that AP feeding may reduce the development of chemically-induced rat colon carcinogenesis.
authors.affiliation: UNESP - Univ. Estadual Paulista, School of Medicine, Program of Post-Graduation in Pathology, Botucatu 18618-970, SP, Brazil.
authors.forename: Mariana F
authors.initials: MF
authors.lastname: Fragoso
authors.forename: Guilherme R
authors.initials: GR
authors.lastname: Romualdo
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Ribeiro
authors.forename: Luis F
authors.initials: LF
authors.lastname: Barbisan
mesh_terms: D019813:1,2-Dimethylhydrazine / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis; D028023:Arecaceae; D063646:Carcinogenesis; D002273:Carcinogens / Q000633:toxicity*; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D018031:Connexin 43 / Q000378:metabolism; D051381:Rats; D051176:beta Catenin / Q000378:metabolism
chemical_list: D002273:Carcinogens; D018031:Connexin 43; D051176:beta Catenin; D019813:1,2-Dimethylhydrazine
keywords: Aberrant crypt foci; Açaí pulp; Colon carcinogenesis; Spray-dry