title: Blood type B antigen modulates cell migration through regulating cdc42 expression and activity in HaCaT cells.
abstract: ABO blood group is determined by carbohydrate antigens, called ABH antigens. It has been known that the change of carbohydrate antigen expression, including ABH antigens, has correlation with the tumor metastasis and survival; however, the exact mechanism remains to be elucidated. ABH antigens are expressed not only in blood cells but also in several tissues. In epidermis, ABH antigen is expressed in the uppermost spinous and granular layer. We investigated the role of ABH antigens on the cell migration of HaCaT keratinocytes, which express B antigen. Knock-down of B antigen expression by small interference RNA of FUT1 inhibited HaCaT cell migration. At that time, we found that lamellipodia and actin fiber were also reduced by knock-down of B antigen expression. The transcription of cdc42, a kind of Rho GTPase which plays a key role in actin polymerization, was reduced by down-regulated B antigen expression. Furthermore, the reduced B antigen expression also inhibited the interaction of cdc42 and N-WASP. Collectively, our data provide a clue how ABH antigens regulate the cell migration mechanism.
authors.affiliation: Department of Dermatology, Seoul National University College of Medicine, Medical Research Center, Seoul National University, Medical Research Center, Seoul National University, Seoul, Korea.
authors.forename: Ji-Yong
authors.initials: JY
authors.lastname: Jung
authors.forename: Jang-Hee
authors.initials: JH
authors.lastname: Oh
authors.forename: Dong Hun
authors.initials: DH
authors.lastname: Lee
authors.forename: Serah
authors.initials: S
authors.lastname: Lee
authors.forename: Jin Ho
authors.initials: JH
authors.lastname: Chung
mesh_terms: D000017:ABO Blood-Group System / Q000378:metabolism*; D057134:Antibodies, Neutralizing / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D024042:Collagen Type I / Q000494:pharmacology; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D005647:Fucosyltransferases / Q000378:metabolism; D055785:Gene Knockdown Techniques; D006801:Humans; D038982:Integrin alpha2beta1 / Q000378:metabolism; D015603:Keratinocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D011554:Pseudopodia / Q000187:drug effects / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D014110:Touch / Q000187:drug effects; D014158:Transcription, Genetic / Q000187:drug effects; D014162:Transfection; D014945:Wound Healing / Q000187:drug effects; D020764:cdc42 GTP-Binding Protein / Q000235:genetics / Q000378:metabolism*; D000097631:Galactoside 2-alpha-L-fucosyltransferase
chemical_list: D000017:ABO Blood-Group System; D057134:Antibodies, Neutralizing; D024042:Collagen Type I; D005647:Fucosyltransferases; D038982:Integrin alpha2beta1; D034741:RNA, Small Interfering; D020764:cdc42 GTP-Binding Protein; D000097631:Galactoside 2-alpha-L-fucosyltransferase