title: [Study on optimization of induction system of test-tube tuberous roots from leaves of Rehmannia glutinosa].
abstract: OBJECTIVE
  To study the effect of sucrose and plant growth substances of different concentrations on the induction of test-tube tuberous roots of Rehmannia glutinosa, in order to establish an efficient system for the induction of test-tube tuberous roots from leaves of R. glutinosa.
  
  
  METHODS
  Leaves from test-tube seedlings of 85-5 R. glutinosa were used as explants. After rooting induction, they were transferred to medium with orthogonal design for inducing test-tube tuberous roots of R. glutinosa.
  
  
  CONCLUSIONS
  NAA played a significant role in induction of test-tube tuberous roots of R. glutinosa, followed by sucrose and 6-BA. With leaves from test-tube seedlings as the explants, the optimal medium for inducing test-tube tuberous roots of R. glutinosa was MS + BA 3.0 mg x L(-1) + NAA 0.1 mg x L(-1) + sucrose 7%. The study provides an efficient induction system for studies on artificial seeds and secondary metabolism with test-tube tuberous roots of R. glutinosa.
authors.affiliation: Key Laboratory of Plant Resources and Biology of Anhui Province, School of Life Science, Huaibei Normal University, Huaibei 235000, China. xuejp2000@yahoo.com.cn
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Xue
authors.forename: Tao
authors.initials: T
authors.lastname: Xue
authors.forename: Lan
authors.initials: L
authors.lastname: Guo
authors.forename: Yan-Fang
authors.initials: YF
authors.lastname: Zhu
authors.forename: He-Dong
authors.initials: HD
authors.lastname: Lu
authors.forename: Ai-Min
authors.initials: AM
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Sheng
mesh_terms: D001593:Benzyl Compounds; D004305:Dose-Response Relationship, Drug; D007701:Kinetin / Q000494:pharmacology; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects / Q000254:growth & development*; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development*; D011687:Purines; D032267:Rehmannia / Q000187:drug effects / Q000254:growth & development*; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development; D013395:Sucrose / Q000494:pharmacology; D046509:Tissue Culture Techniques / Q000295:instrumentation / Q000379:methods*
chemical_list: D001593:Benzyl Compounds; D009280:Naphthaleneacetic Acids; D010937:Plant Growth Regulators; D011687:Purines; D013395:Sucrose; C480551:benzylaminopurine; D007701:Kinetin