title: Dynamics of amino acid utilization in Phaeobacter inhibens DSM 17395.
abstract: Time-resolved utilization of multiple amino acids by Phaeobacter inhibens DSM 17395 was studied during growth with casamino acids. The 15 detected amino acids could be grouped according to depletion rate into four different categories, i.e. from rapid (category I) to nondepletion (category IV). Upon entry into stationary growth phase, amino acids of category I (e.g. glutamate) were (almost) completely depleted, while those of categories II (e.g. leucine) and III (e.g. serine) were further consumed at varying rates and to different extents. Thus, cultures entered stationary growth phase despite the ample presence of organic nutrients, i.e. under nonlimiting conditions. Integrated proteomic and metabolomic analysis identified 1747 proteins and 94 intracellular metabolites. Of these, 180 proteins and 86 metabolites displayed altered abundance levels during growth. Most strikingly, abundance and activity profiles of alanine dehydrogenase concomitantly increased with the onset of enhanced alanine utilization during transition into stationary growth phase. Most enzymes of amino acid and central metabolism, however, displayed unaltered abundances across exponential and stationary growth phases. In contrast, metabolites of the Entner-Doudoroff pathway and gluconeogenesis as well as cellular fatty acids increased markedly in abundance in early stationary growth phase.
authors.affiliation: Institute for Chemistry and Biology of the Marine Environment (ICBM), Carl von Ossietzky University Oldenburg, Oldenburg, Germany.
authors.forename: Hajo
authors.initials: H
authors.lastname: Zech
authors.forename: Michael
authors.initials: M
authors.lastname: Hensler
authors.forename: Sebastian
authors.initials: S
authors.lastname: Koßmehl
authors.forename: Katharina
authors.initials: K
authors.lastname: Drüppel
authors.forename: Lars
authors.initials: L
authors.lastname: Wöhlbrand
authors.forename: Kathleen
authors.initials: K
authors.lastname: Trautwein
authors.forename: Thomas
authors.initials: T
authors.lastname: Colby
authors.forename: Jürgen
authors.initials: J
authors.lastname: Schmidt
authors.forename: Richard
authors.initials: R
authors.lastname: Reinhardt
authors.forename: Kerstin
authors.initials: K
authors.lastname: Schmidt-Hohagen
authors.forename: Dietmar
authors.initials: D
authors.lastname: Schomburg
authors.forename: Ralf
authors.initials: R
authors.lastname: Rabus
mesh_terms: D000596:Amino Acids / Q000096:biosynthesis / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism; D003470:Culture Media / Q000494:pharmacology; D030562:Databases, Protein; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D055432:Metabolomics; D020543:Proteome / Q000378:metabolism; D040901:Proteomics; D041904:Roseobacter / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; D003470:Culture Media; D020543:Proteome
keywords: Casamino acids; Catabolic Dynamics; Metabolomics; Microbiology; Phaeobacter inhibens; Physiology