title: Systems proteomics of healthy and diseased chromatin.
abstract: Differences in chromatin-associated proteins allow the same genome to participate in multiple cell types and to respond to an array of stimuli in any given cell. To understand the fundamental properties of chromatin and to reveal its cell- and/or stimulus-specific behaviors, quantitative proteomics is an essential technology. This chapter details the methods for fractionation and quantitative mass spectrometric analysis of chromatin from hearts or isolated adult myocytes, detailing some of the considerations for applications to understanding heart disease. The state-of-the-art methodology for data interpretation and integration through bioinformatics is reviewed.
authors.affiliation: Department of Anesthesiology, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA.
authors.forename: Haodong
authors.initials: H
authors.lastname: Chen
authors.forename: Emma
authors.initials: E
authors.lastname: Monte
authors.forename: Thomas M
authors.initials: TM
authors.lastname: Vondriska
authors.forename: Sarah
authors.initials: S
authors.lastname: Franklin
mesh_terms: D000818:Animals; D002318:Cardiovascular Diseases / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D002467:Cell Nucleus / Q000737:chemistry / Q000378:metabolism; D005591:Chemical Fractionation; D002843:Chromatin / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology; D019295:Computational Biology; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D006801:Humans; D013058:Mass Spectrometry; D051379:Mice; D009206:Myocardium / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology; D032383:Myocytes, Cardiac / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D059748:Proteolysis; D020543:Proteome / Q000235:genetics / Q000378:metabolism*
chemical_list: D002843:Chromatin; D020543:Proteome