title: Enhanced lipidic algae biomass production using gas transfer from a fermentative Rhodosporidium toruloides culture to an autotrophic Chlorella protothecoides culture.
abstract: In order to produce single-cell oil for biodiesel, a yeast and a microalga were, for the first time, grown in two separate reactors connected by their gas-phases, taking advantage of their complementary nutritional metabolisms, i.e., respiration and photosynthesis. The yeast Rhodosporidium toruloides was used for lipid production, originating a carbon dioxide-enriched outlet gas stream which in turn was used to stimulate the autotrophic growth of Chlorella protothecoides in a vertical-alveolar-panel (VAP) photobioreactor. The microalgal biomass productivity was 0.015 gL(-1)h(-1), and its lipid productivity attained 2.2 mgL(-1)h(-1) when aerated with the outlet gas stream from the yeast fermenter. These values represent an increase of 94% and 87%, respectively, as compared to a control culture aerated with air. The CO2 bio-fixed by the microalgal biomass reached an estimated value of 29 mgL(-1)h(-1) in the VAP receiving the gas stream from the fermenter, a value 1.9 times higher than that measured in the control VAP.
authors.affiliation: LNEG, U. Bioenergia, edificio F, Estrada do Paço do Lumiar, 22, 1649-038 Lisbon, Portugal.
authors.forename: C A
authors.initials: CA
authors.lastname: Santos
authors.forename: M L
authors.initials: ML
authors.lastname: Caldeira
authors.forename: T
authors.initials: T
authors.lastname: Lopes da Silva
authors.forename: J M
authors.initials: JM
authors.lastname: Novais
authors.forename: A
authors.initials: A
authors.lastname: Reis
mesh_terms: D064751:Ammonium Compounds / Q000378:metabolism; D052818:Autotrophic Processes; D001487:Basidiomycota / Q000254:growth & development* / Q000378:metabolism; D018533:Biomass; D002245:Carbon Dioxide / Q000378:metabolism; D018929:Cell Culture Techniques; D002462:Cell Membrane / Q000378:metabolism; D002708:Chlorella / Q000254:growth & development*; D005227:Fatty Acids / Q000378:metabolism; D005285:Fermentation; D005434:Flow Cytometry; D005740:Gases / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D052836:Heterotrophic Processes; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008055:Lipids / Q000737:chemistry*; D010100:Oxygen / Q000378:metabolism; D058433:Photobioreactors / Q000382:microbiology; D013997:Time Factors
chemical_list: D064751:Ammonium Compounds; D005227:Fatty Acids; D005740:Gases; D008055:Lipids; D002245:Carbon Dioxide; D005947:Glucose; D010100:Oxygen