title: In vivo NADH fluorescence imaging indicates effect of aquaporin-4 deletion on oxygen microdistribution in cortical spreading depression.
abstract: Using in vivo two-photon imaging, we show that mice deficient in aquaporin-4 (AQP4) display increased fluorescence of nicotinamide adenine dinucleotide (NADH) when subjected to cortical spreading depression. The increased NADH signal, a proxy of tissue hypoxia, was restricted to microwatershed areas remote from the vasculature. Aqp4 deletion had no effects on the hyperemia response, but slowed [K(+)]o recovery. These observations suggest that K(+) uptake is suppressed in Aqp4(-/-) mice as a consequence of decreased oxygen delivery to tissue located furthest away from the vascular source of oxygen, although increased oxygen consumption may also contribute to our observations.
authors.affiliation: Division of Glial Disease and Therapeutics, Center for Translational Neuromedicine, University of Rochester Medical Center, Rochester, New York, USA.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Thrane
authors.forename: Takahiro
authors.initials: T
authors.lastname: Takano
authors.forename: Vinita
authors.initials: V
authors.lastname: Rangroo Thrane
authors.forename: Fushun
authors.initials: F
authors.lastname: Wang
authors.forename: Weiguo
authors.initials: W
authors.lastname: Peng
authors.forename: Ole Petter
authors.initials: OP
authors.lastname: Ottersen
authors.forename: Maiken
authors.initials: M
authors.lastname: Nedergaard
authors.forename: Erlend A
authors.initials: EA
authors.lastname: Nagelhus
mesh_terms: D000818:Animals; D051401:Aquaporin 4 / Q000235:genetics / Q000502:physiology*; D002560:Cerebrovascular Circulation / Q000187:drug effects / Q000502:physiology; D013181:Cortical Spreading Depression / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D005260:Female; D017353:Gene Deletion; D002534:Hypoxia, Brain / Q000378:metabolism*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D008954:Models, Biological; D009243:NAD / Q000737:chemistry / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D010101:Oxygen Consumption / Q000502:physiology; D011189:Potassium Chloride / Q000378:metabolism / Q000494:pharmacology
chemical_list: C496031:Aqp4 protein, mouse; D051401:Aquaporin 4; D009243:NAD; D011189:Potassium Chloride; D010100:Oxygen