title: Bluetongue virus RNA detection by real-time rt-PCR in post-vaccination samples from cattle.
abstract: Bluetongue virus serotype 8 (BTV-8) was responsible for a large outbreak among European ruminant populations in 2006-2009. In spring 2008, a massive vaccination campaign was undertaken, leading to the progressive disappearance of the virus. During surveillance programmes in Western Europe in 2010-2011, a low but significant number of animals were found weakly positive using BTV-specific real-time RT-PCR, raising questions about a possible low level of virus circulation. An interference of the BTV-8 inactivated vaccine on the result of the real-time RT-PCR was also hypothesized. Several studies specifically addressed the potential association between a recent vaccination and BTV-8 RNA detection in the blood of sheep. Results were contradictory and cattles were not investigated. To enlighten this point, a large study was performed to determine the risks of detection of bluetongue vaccine-associated RNA in the blood and spleen of cattle using real-time RT-PCR. Overall, the results presented clearly demonstrate that vaccine viral RNA can reach the blood circulation in sufficient amounts to be detected by real-time RT-PCR in cattle. This BTV-8 vaccine RNA carriage appears as short lasting.
authors.affiliation: Department of Virology, Unit of Vesicular and Exotic Diseases, CODA-CERVA, Ukkel, Belgium.
authors.forename: I
authors.initials: I
authors.lastname: De Leeuw
authors.forename: M
authors.initials: M
authors.lastname: Garigliany
authors.forename: G
authors.initials: G
authors.lastname: Bertels
authors.forename: T
authors.initials: T
authors.lastname: Willems
authors.forename: D
authors.initials: D
authors.lastname: Desmecht
authors.forename: K
authors.initials: K
authors.lastname: De Clercq
mesh_terms: D000818:Animals; D001819:Bluetongue / Q000453:epidemiology* / Q000517:prevention & control / Q000821:virology; D001820:Bluetongue virus / Q000235:genetics / Q000302:isolation & purification*; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control* / Q000821:virology*; D005060:Europe / Q000453:epidemiology; D012367:RNA, Viral / Q000032:analysis*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D014611:Vaccination / Q000379:methods / Q000662:veterinary*; D015164:Vaccines, Inactivated / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology
chemical_list: D012367:RNA, Viral; D015164:Vaccines, Inactivated; D014765:Viral Vaccines
keywords: RNA detection; bluetongue; disease freedom; post-vaccination; vaccine