title: Profiling of jasmonic acid-related metabolites and hormones in wounded leaves.
abstract: The endogenous concentration of N-jasmonoyl-L-isoleucine (JA-Ile) is regulated by the balance between biosynthesis and deactivation and controls plant developmental processes and stress responses. Therefore, profiling of its precursors and metabolites is required to understand the mechanism by which the JA-Ile concentration is regulated. Also, other hormones, such as indole-3-acetic acid, abscisic acid, salicylic acid, and ethylene, have been suggested to interact with JA-Ile signaling. Profiling of these hormones and their metabolites should give us insights into their interaction mode. Liquid chromatography-electrospray ionization-tandem mass spectrometry has enabled us to develop a highly sensitive and high-throughput comprehensive quantification analysis of phytohormones.
authors.affiliation: Life Science Group, Agilent Technologies, Tokyo, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Jikumaru
authors.forename: Mitsunori
authors.initials: M
authors.lastname: Seo
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Matsuura
authors.forename: Yuji
authors.initials: Y
authors.lastname: Kamiya
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D003517:Cyclopentanes / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D055442:Metabolome; D054883:Oxylipins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism*; D012015:Reference Standards; D021241:Spectrometry, Mass, Electrospray Ionization / Q000592:standards; D053719:Tandem Mass Spectrometry / Q000592:standards
chemical_list: D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; C011006:jasmonic acid