title: [Study on the correlation between membrane protein Flotillin-1 and PrPc endocytosis].
abstract: OBJECTIVE
  To explore whether the membrane-associated protein Flotillin-1 has relationship with endocytosis of PrPc.
  
  
  METHODS
  The expression of Flotillin-1 in different cell lines was detected with the method of Western Blot; the interaction between Flotillin-1 and PrPc in Cells which were treated with copper ions was observed using immunoprecipitation method.
  
  
  RESULTS
  (1) Flotillin-1 was widely expressed in many cell lines without significant difference in the amounts of expression level; (2) Only in the appearance of copper ions, the protein complexes of PrPc and Flotillin-1 can be detected with the method of IP, which were related to copper ions concentration and processing time.
  
  
  CONCLUSIONS
  The membrane-associated protein Flotillin-1 has the relationship with the endocytosis of PrPc.
authors.affiliation: State key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Ren
authors.forename: Ke
authors.initials: K
authors.lastname: Wang
authors.forename: Yin
authors.initials: Y
authors.lastname: Xu
authors.forename: Long-Zhu
authors.initials: LZ
authors.lastname: Li
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Yu-E
authors.initials: YE
authors.lastname: Yan
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Dong
authors.forename: Chen
authors.initials: C
authors.lastname: Gao
mesh_terms: D002460:Cell Line; D002462:Cell Membrane / Q000235:genetics / Q000378:metabolism; D004705:Endocytosis; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D018621:PrPC Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D021381:Protein Transport
chemical_list: D008565:Membrane Proteins; D018621:PrPC Proteins; C106354:flotillins