title: Altered phenotype and function of dendritic cells in individuals with chronic periodontitis.
abstract: OBJECTIVE
  To investigate the effects of periodontal bacterial lysates on maturation and function of mature monocyte-derived dendritic cells (m-MDDCs) derived from individuals with chronic periodontitis (CP) or healthy periodontal tissue (HP).
  
  
  METHODS
  m-MDDCs derived from peripheral blood monocytes, cultured for 7 days in presence of interleukin (IL)-4 and granulocyte-macrophage colony stimulating factor (GM-CSF), were stimulated with lysates of Streptococcus sanguinis, Prevotella intermedia, Porphyromonas gingivalis, or Treponema denticola on day 4, and were then phenotyped. IL-10, IL-12 and IFN-gamma concentration in the supernatant of cultures were measured.
  
  
  RESULTS
  Expression of HLA-DR was lower in bacterial-unstimulated mature m-MDDC from CP compared to HP (p=0.04), while expression of CD1a and CD123 were higher in CP. The expression pattern of HLA-DR, CD11c, CD123, and CD1a did not change on bacterial stimulation, regardless of the bacteria. Stimulation with P. intermedia upregulated CD80 and CD86 in CP cells (p≤0.05). Production of IL-12p70 by bacterial-unstimulated m-MDDCs was 5.8-fold greater in CP compared to HP. Bacterial stimulation further increased IL-12p70 production while decreasing IL-10. Significantly more IFN-gamma was produced in co-cultures of CP m-MDDCs than with HP m-MDDCs when cells were stimulated with P. intermedia (p=0.009).
  
  
  CONCLUSIONS
  Bacterial-unstimulated m-MDDC from CP exhibited a more immature phenotype but a cytokine profile biased towards proinflammatory response; this pattern was maintained/exacerbated after bacterial stimulation. P. intermedia upregulated co-stimulatory molecules and IFN-gamma expression in CP m-MDDC. These events might contribute to periodontitis pathogenesis.
authors.affiliation: Department of Periodontics, School of Dentistry of the Federal University of Bahia, Avenue Araujo Pinho 62, Canela, Salvador, Bahia, Brazil. patricia.cury@ufba.br
authors.forename: P R
authors.initials: PR
authors.lastname: Cury
authors.forename: J P
authors.initials: JP
authors.lastname: Carmo
authors.forename: V V
authors.initials: VV
authors.lastname: Horewicz
authors.forename: J N
authors.initials: JN
authors.lastname: Santos
authors.forename: J A
authors.initials: JA
authors.lastname: Barbuto
mesh_terms: D015703:Antigens, CD / Q000378:metabolism*; D002457:Cell Extracts; D055113:Chronic Periodontitis / Q000097:blood / Q000235:genetics* / Q000276:immunology; D018920:Coculture Techniques; D016207:Cytokines / Q000096:biosynthesis*; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism*; D005434:Flow Cytometry; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000276:immunology*; D006684:HLA-DR Antigens / Q000378:metabolism*; D006801:Humans; D015847:Interleukin-4 / Q000276:immunology*; D010519:Periodontium / Q000382:microbiology / Q000473:pathology; D010641:Phenotype; D016966:Porphyromonas gingivalis / Q000472:pathogenicity; D018713:Prevotella intermedia / Q000472:pathogenicity; D013298:Streptococcus sanguis / Q000472:pathogenicity; D048373:Treponema denticola / Q000472:pathogenicity
chemical_list: D015703:Antigens, CD; D002457:Cell Extracts; D016207:Cytokines; D006684:HLA-DR Antigens; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor
keywords: Immune response; Periodontal bacteria; Periodontal disease