title: MicroRNA-17/20a functions to inhibit cell migration and can be used a prognostic marker in oral squamous cell carcinoma.
abstract: OBJECTIVE
  Oral squamous cell carcinoma (OSCC) accounts for>90% oral cancer which is a leading cause of cancer death worldwide. Early diagnosis may well offer an opportunity to increase survival to this neoplasm. Micro(mi)RNA-interfered cancer progression is crucial, yet its migration machinery of OSCC is still unknown. To access whether the possible miRNA prognostic markers and underlying mechanisms, we developed a highly migratory TW2.6 MS-10 cells from TW2.6 cells to investigate the issue.
  
  
  METHODS
  miRNA profiling was performed on TW2.6 and TW2.6 MS-10. Target miRNA was correlated to pathological status in OSCC patients by real-time RT-PCR. A downstream effector was identified using a bioinformatics analysis, and a 3'-untranslated region (UTR) reporter assay was used.
  
  
  RESULTS
  An miRNA cluster, miR-17-92, including miR-17, miR-19b, miR-20a, and miR-92a, was found to be significantly down-regulated in TW2.6 MS-10 compared to TW2.6 cells. Overexpression of this cluster decreased the migratory ability of OSCC cell lines. We further demonstrated that miR-17 and miR-20a are the main miRNAs of miR-17-92 cluster which modulate OSCC migration. Clinically, miR-17/20a showed negative correlation with TNM stage and lymphatic metastasis. Through a bioinformatics screening analysis and 3'UTR reporter assay, we confirmed the integrin (ITG) β8 as a direct target of miR-17/20a, and knockdown of ITGβ8 reduced cell migratory capability of OSCC.
  
  
  CONCLUSIONS
  miR-17/20a acts as a prognostic predictor of OSCC patients' outcome and a tumor migration suppressor miRNA.
authors.affiliation: Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan; Graduate Institute of Oral Biology, School of Dentistry, National Taiwan University, Taipei, Taiwan.
authors.forename: Cheng-Chi
authors.initials: CC
authors.lastname: Chang
authors.affiliation: Graduate Institute of Oral Biology, School of Dentistry, National Taiwan University, Taipei, Taiwan.
authors.forename: Yu-Jen
authors.initials: YJ
authors.lastname: Yang
authors.affiliation: Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan.
authors.forename: Yue-Ju
authors.initials: YJ
authors.lastname: Li
authors.affiliation: Department of Pediatrics, National Taiwan University Hospital Yun-Lin Branch, Yunlin County, Taiwan; Department of Pediatrics, National Taiwan University Hospital, Taipei, Taiwan.
authors.forename: Szu-Ta
authors.initials: ST
authors.lastname: Chen
authors.affiliation: Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan.
authors.forename: Been-Ren
authors.initials: BR
authors.lastname: Lin
authors.affiliation: Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan.
authors.forename: Tai-Sheng
authors.initials: TS
authors.lastname: Wu
authors.affiliation: Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan; Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan.
authors.forename: Sze-Kwen
authors.initials: SK
authors.lastname: Lin
authors.affiliation: Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan; Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan.
authors.forename: Mark Yen-Ping
authors.initials: MY
authors.lastname: Kuo
authors.affiliation: Department of Otolaryngology, National Taiwan University Hospital, Taipei, Taiwan. Electronic address: tanct5222@ntu.edu.tw.
authors.forename: Ching-Ting
authors.initials: CT
authors.lastname: Tan
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement; D016000:Cluster Analysis; D006801:Humans; D035683:MicroRNAs / Q000502:physiology*; D009062:Mouth Neoplasms / Q000235:genetics / Q000473:pathology*; D011379:Prognosis
chemical_list: C524941:MIRN17 microRNA, human; C534451:MIRN20a microRNA, human; D035683:MicroRNAs
keywords: 3′ untranslated region; 3′UTR; CT; IPA; ITGβ8; LAMA3; Migration; OSCC; Oral squamous cell carcinoma; ROC; comparative threshold; ingenuity pathway analysis; integrin β8; laminin α3; miRNA; miRNA-17; miRNA-20a; microRNA; oral squamous cell carcinoma; receiver-operating characteristics