title: A novel anti-human ICOSL monoclonal antibody that enhances IgG production of B cells.
abstract: ICOSL, a newly identified member of the B7 superfamily, plays a major role in immune responses. In this study, a functional anti-human ICOSL monoclonal antibody (MAb) 3B3 was obtained and characterized by means of flow cytometry, Western blot, and competition assay. This MAb could specifically recognize a distinct epitope of the ICOSL molecule. As a functional antibody, MAb 3B3 could inhibit the proliferation of T lymphocytes stimulated by ICOSL-L929 transfectants. Furthermore, it could enhance IgG production of PWM-driven B cells. The results indicate that the ICOS-ICOSL signal is critically involved in specific humoral immunity.
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Fengming
authors.initials: F
authors.lastname: Wang
authors.forename: Qiuping
authors.initials: Q
authors.lastname: Cai
authors.forename: Shuang
authors.initials: S
authors.lastname: Shen
authors.forename: Youguo
authors.initials: Y
authors.lastname: Chen
authors.forename: Chao
authors.initials: C
authors.lastname: Hao
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
mesh_terms: D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000494:pharmacology*; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D001667:Binding, Competitive; D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018604:Epitope Mapping; D006801:Humans; D006825:Hybridomas; D056724:Immunity, Humoral; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000276:immunology; D060945:Inducible T-Cell Co-Stimulator Ligand / Q000037:antagonists & inhibitors / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D011043:Pokeweed Mitogens / Q000276:immunology / Q000494:pharmacology; D011485:Protein Binding; D013601:T-Lymphocytes / Q000187:drug effects / Q000502:physiology
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; C498514:ICOSLG protein, human; D007074:Immunoglobulin G; D060945:Inducible T-Cell Co-Stimulator Ligand; D011043:Pokeweed Mitogens