title: Identification and characterization of the interaction between viroplasm-associated proteins from two different plant-infecting reoviruses and eEF-1A of rice.
abstract: A rice protein homologous to eukaryotic translation elongation factor 1A (eEF-1A) was found to interact with the Pns6 of rice ragged stunt virus (RRSV), the type member of the genus Oryzavirus, family Reoviridae, in yeast two-hybrid screening. The interaction between the rice protein, designated OseEF-1A, and RRSV Pns6 was confirmed by bimolecular fluorescence complementation. Besides Pns6, OseEF-1A also interacted with the viroplasm matrix protein, Pns10, of RRSV. When expressed together, OseEF-1A co-localized with RRSV Pns10 in epidermal cells of Nicotiana benthamiana. Pns6 of southern rice black-streaked dwarf virus (SRBSDV), a newly reported member of the genus Fijivirus, family Reoviridae, was the only non-structural SRBSDV protein studied here that also interacted with OseEF-1A. Like Pns6 of rice black-streaked dwarf virus (RBSDV), SRBSDV Pns6 interacted with itself and co-localized with Pns9-1 in N. benthamiana. In the presence of Pns6, OseEF-1A co-localized with Pns9-1, the putative viroplasm matrix protein of SRBSDV.
authors.affiliation: Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Songbai
authors.forename: Du
authors.initials: D
authors.lastname: Zhenguo
authors.forename: Yang
authors.initials: Y
authors.lastname: Liang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhengjie
authors.forename: Wu
authors.initials: W
authors.lastname: Kangcheng
authors.forename: Li
authors.initials: L
authors.lastname: Guangpu
authors.forename: Wu
authors.initials: W
authors.lastname: Zujian
authors.forename: Xie
authors.initials: X
authors.lastname: Lianhui
mesh_terms: D060054:Agrobacterium; D002478:Cells, Cultured; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D012275:Oryza / Q000821:virology*; D010942:Plant Viruses / Q000235:genetics / Q000378:metabolism*; D012087:Reoviridae / Q000235:genetics / Q000378:metabolism*; D014026:Nicotiana / Q000166:cytology / Q000821:virology; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D014764:Viral Proteins