title: [Study on optimization of SRAP-PCR reaction system for Pinellia ternata in Suzhou].
abstract: OBJECTIVE
  To investigate the optimization system of SRAP-PCR molecular marker technology in the analysis on Pinellia ternata.
  
  
  METHODS
  SRAP-PCR reaction system for P. ternata was optimized by L16 (5(4)) orthogonal design with five elements (dNTPs, Mg2+, the template DNA, primers, Taq enzyme) and four standards.
  
  
  RESULTS
  The most suitable forward primer for SRAP for Pinellia ternata was 5'-TGAGTCCAAACCGGAAG-3', while the reverse primer was 5'-GACTGCGTACGAATTACG-3'. The optimized reaction system contained 70 ng DNA template, 0.9 micromol x L(-1) primer, 0.20 mmol x L(-1) dNTP s, 1.5 - 2.0 mmol x L(-1) Mg2+, and 2 U Taq enzyme.
  
  
  CONCLUSIONS
  SRAP-PCR system for P. ternata is established to lay a foundation for future construction of SRAP genetic map of P. ternata.
authors.affiliation: Anhui Key Laboratory of Plant Resources and Biology, School of Life Science, Huaibei Normal University, Huaibei 235000, China.
authors.forename: Ai-Min
authors.initials: AM
authors.lastname: Zhang
authors.forename: He-Dong
authors.initials: HD
authors.lastname: Lu
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Xue
authors.forename: Xing-Kui
authors.initials: XK
authors.lastname: Tao
authors.forename: Tao
authors.initials: T
authors.lastname: Xue
authors.forename: Wei
authors.initials: W
authors.lastname: Sheng
authors.forename: Yan-Fang
authors.initials: YF
authors.lastname: Zhu
mesh_terms: D002681:China; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics*; D004586:Electrophoresis; D008274:Magnesium / Q000378:metabolism; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D009711:Nucleotides / Q000235:genetics; D030021:Pinellia / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D019914:Taq Polymerase / Q000378:metabolism; D013698:Templates, Genetic
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D009711:Nucleotides; D019914:Taq Polymerase; D008274:Magnesium