title: Oceanobacillus chungangensis sp. nov., isolated from a sand dune.
abstract: A Gram-stain-positive, spore-forming, rod-shaped, motile, strictly aerobic bacterial strain, designated CAU 1051(T), was isolated from a sand dune and its taxonomic position was investigated using a polyphasic approach. Strain CAU 1051(T) grew optimally at pH 5.0 and 30 °C. NaCl was not required for growth but up to 10.0 % (w/v) NaCl was tolerated. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CAU 1051(T) formed a distinct lineage within the genus Oceanobacillus and was most closely related to Oceanobacillus profundus CL-MP28(T), Oceanobacillus caeni S-11(T), and Oceanobacillus picturae LMG 19492(T) (96.8 %, 95.6 % and 95.3 % similarity, respectively). DNA-DNA reassociation analysis showed that strain CAU 1051(T) displayed 28.2±0.7 % relatedness to O. profundus KCTC 13625(T). Strain CAU 1051(T) contained MK-7 as the only isoprenoid quinone and anteiso-C15 : 0 as the major fatty acid. The cell wall peptidoglycan of strain CAU 1051(T) contained meso-diaminopimelic acid. The polar lipids were composed of diphosphatidylglycerol, phosphatidylglycerol, six unidentified phospholipids, an unidentified glycolipid, and six unidentified polar lipids. The major whole-cell sugars were glucose and ribose. The DNA G+C content was 36.3 mol%. On the basis of phenotypic data and phylogenetic inference, strain CAU 1051(T) represents a novel species of the genus Oceanobacillus for which the name Oceanobacillus chungangensis sp. nov. is proposed. The type strain is CAU 1051(T) ( = KCTC 33035(T) = CCUG 63270(T)).
authors.affiliation: Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
authors.forename: Dong Chae
authors.initials: DC
authors.lastname: Lee
authors.affiliation: Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
authors.forename: Hyeonji
authors.initials: H
authors.lastname: Kang
authors.affiliation: Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
authors.forename: Veeraya
authors.initials: V
authors.lastname: Weerawongwiwat
authors.affiliation: Department of Dermatology, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
authors.forename: Beomjoon
authors.initials: B
authors.lastname: Kim
authors.affiliation: School of Electrical and Electronics, Chung-Ang University College of Engineering, Seoul, Republic of Korea.
authors.forename: Young-Wan
authors.initials: YW
authors.lastname: Choi
authors.affiliation: Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
authors.forename: Wonyong
authors.initials: W
authors.lastname: Kim
mesh_terms: D001406:Bacillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012822:Silicon Dioxide; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid; D012822:Silicon Dioxide; C062629:menaquinone 7