title: More about sampling and estimation of mercaptans in air samples.
abstract: Several strategies have been developed for sampling and determination of volatile thiols. The selectivity and sensitivity of the proposed methodologies are achieved by using a specific derivatizing reagent. The different procedures assayed are based on air sampling followed by derivatization of the analytes with OPA and isoleucine in alkaline solution. The derivatization products are separated and determined by liquid chromatography and fluorescence detection. To start, the derivatization conditions and stability of the derivates have been studied in order to establish the storage conditions. In general, the strategies studied consisted on trapping and detivatization the thiol compound on different support; a solution (Impinger) or sorbent (C₁₈ cartridges or glass fiber filter). The analytical properties of the different strategies have been obtained and compared. Procedures are recommended upon specific situations.
authors.affiliation: Departamento de Química Analítica, Facultad de Química, Universidad de Valencia, C/Dr. Moliner 50, - Burjassot, Valencia E46100, Spain.
authors.forename: Y
authors.initials: Y
authors.lastname: Moliner-Martínez
authors.forename: R
authors.initials: R
authors.lastname: Herráez-Hernández
authors.forename: C
authors.initials: C
authors.lastname: Molins-Legua
authors.forename: J
authors.initials: J
authors.lastname: Verdú-Andrés
authors.forename: M
authors.initials: M
authors.lastname: Avella-Oliver
authors.forename: P
authors.initials: P
authors.lastname: Campíns-Falcó
mesh_terms: D000388:Air / Q000032:analysis*; D000393:Air Pollutants / Q000032:analysis*; D000468:Alkalies; D002138:Calibration; D002853:Chromatography, Liquid; D005456:Fluorescent Dyes; D006801:Humans; D007532:Isoleucine / Q000737:chemistry; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013438:Sulfhydryl Compounds / Q000032:analysis*; D009764:o-Phthalaldehyde / Q000737:chemistry
chemical_list: D000393:Air Pollutants; D000468:Alkalies; D005456:Fluorescent Dyes; D012996:Solutions; D013438:Sulfhydryl Compounds; D007532:Isoleucine; D009764:o-Phthalaldehyde