title: Biological significance of the overexpression of HSP70 and alpha B-crystallin in rat substantia nigra exposed to different doses of permethrin.
abstract: The aim of this study was to investigate the possible role of the Heat Shock Protein 70 (HSP70) and Alpha B-crystallin (αBC) in the substantia nigra of rats exposed to permethrin at different doses on the apoptotic cell status. The orogastric gavage method was used to administer the different doses of permethrin (75 mg kg-1 in Group I, 150 mg kg-1 in group II, 300 mg kg-1 in group III) to the rats. Using the Western blot test, all the permethrin-treated groups showed a dose-dependent increase in the expression of HSP70 and αBC when compared to the control group. TUNEL positive apoptotic cells were not detected in the dopaminergic neurons of the substantia nigra after treatment with permethrin; however, upon immunofluorescent staining, intense positive reactions for HSP70 and αBC were observed in all of the treated groups. No immunopositive cells were detected in the tissue sections of the control group. These results suggest that the different administered doses of permethrin did not cause apoptotic cell death in the substantia nigra dopaminergic neurons; however, they did induce an increase in HSP70 and αBC expression. Thus, it appears that HSP70 and αBC could play a neuroprotective role in permethrin-induced neurotoxicity.
authors.affiliation: Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Ondokuz Mayis, Samsun, Turkey. dguvenc@omu.edu.tr
authors.forename: Dilek
authors.initials: D
authors.lastname: Guvenc
authors.forename: Yonca Betil
authors.initials: YB
authors.lastname: Kabak
authors.forename: Enes
authors.initials: E
authors.lastname: Atmaca
authors.forename: Abdurrahman
authors.initials: A
authors.lastname: Aksoy
authors.forename: Tolga
authors.initials: T
authors.lastname: Guvenc
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002801:Cholinesterase Reactivators / Q000633:toxicity*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004781:Environmental Exposure; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D008297:Male; D018696:Neuroprotective Agents / Q000378:metabolism; D020258:Neurotoxicity Syndromes / Q000209:etiology*; D026023:Permethrin / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013378:Substantia Nigra / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D038203:alpha-Crystallin B Chain / Q000378:metabolism*
chemical_list: D002801:Cholinesterase Reactivators; D018840:HSP70 Heat-Shock Proteins; D018696:Neuroprotective Agents; D038203:alpha-Crystallin B Chain; D026023:Permethrin