title: Zinc modulation of calcium activity at the photoreceptor terminal: a calcium imaging study.
abstract: There is abundant experimental evidence that zinc ions (Zn(2+)) are present in the synaptic vesicles of vertebrate photoreceptors, and that they are co-released with glutamate. Here we show that increasing the concentration of extracellular zinc (2 μM-2 mM) suppresses the entry of calcium into the synaptic terminals of isolated salamander double cones. The resultant dose-dependent curve was fit by an inverse Hill equation having an IC50 of 38 μM, and Hill coefficient of 1.1. Because there is currently no reliable way to measure the concentration of extracellular zinc, it is not known whether the zinc released under normal circumstances is of physiological significance. In an attempt to circumvent this problem we used zinc chelators to reduce the available pool of endogenous zinc. This enabled us to determine how the absence of zinc affected calcium entry. We found that when intra- or extra-cellular zinc was chelated by 250 μM of membrane-permeable TPEN or 500 μM of membrane-impermeable histidine, there was a significant rise in the depolarization-induced intracellular calcium level within photoreceptor terminals. This increase in internal [Ca(2+)] will undoubtedly lead to a concomitant increase in glutamate release. In addition, we found that blocking the L-type calcium channels that are expressed on the synaptic terminals of photoreceptors with 50 μM nicardipine or 100 μM verapamil abolished the effects of zinc chelation. These findings are a good indication that, when released in vivo, the zinc concentration is sufficient to suppress voltage-gated calcium channels, and reduce the rate of glutamate release from photoreceptor terminals.
authors.affiliation: Department of Biological Sciences, Hunter College and the Graduate Center, CUNY, 695 Park Avenue, New York, NY 10065, USA. ianastassov77@gmail.com
authors.forename: Ivan
authors.initials: I
authors.lastname: Anastassov
authors.forename: Wen
authors.initials: W
authors.lastname: Shen
authors.forename: Harris
authors.initials: H
authors.lastname: Ripps
authors.forename: Richard L
authors.initials: RL
authors.lastname: Chappell
mesh_terms: D000557:Ambystoma; D000814:Aniline Compounds / Q000378:metabolism; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D020746:Calcium Channels, L-Type / Q000187:drug effects / Q000378:metabolism; D002614:Chelating Agents / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005029:Ethylenediamines / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000378:metabolism; D018698:Glutamic Acid / Q000378:metabolism; D006639:Histidine / Q000494:pharmacology; D008856:Microscopy, Fluorescence; D017729:Presynaptic Terminals / Q000187:drug effects* / Q000378:metabolism; D017949:Retinal Cone Photoreceptor Cells / Q000187:drug effects* / Q000378:metabolism; D014966:Xanthenes / Q000378:metabolism; D015032:Zinc / Q000494:pharmacology*
chemical_list: D000814:Aniline Compounds; D002121:Calcium Channel Blockers; D020746:Calcium Channels, L-Type; D002614:Chelating Agents; D005029:Ethylenediamines; C409648:Fluo 4; D005456:Fluorescent Dyes; D014966:Xanthenes; D018698:Glutamic Acid; D006639:Histidine; D015032:Zinc; C044387:N,N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine; D002118:Calcium