title: Effect of mesenchymal stem cells on anti-Thy1,1 induced kidney injury in albino rats.
abstract: OBJECTIVE
  To evaluate the effect of mesenchymal stem cells (MSCs) in rats with anti-Thy1,1 nephritis.
  
  
  METHODS
  Female albino rats were divided into three groups, control group, anti-Thy1,1 group and treatment with i.v. MSCs group. MSCs were derived from bone marrow of male albino rats, Y-chromosome gene was detected by polymerase chain reaction in the kidney. Serum urea and creatinine were estimated for all groups. Kidney of all studied groups was examined histologically and histochemically (total carbohydrates and total proteins). DNA fragmentation and expression of α-SMA were detected.
  
  
  RESULTS
  Kidney of animals injected with anti-Thy1,1 showed inflammatory leucocytic infiltration, hypertrophied glomeruli, tubular necrosis and congestion in the renal blood vessels. The kidney tissue also showed reduction of carbohydrates and total proteins together with increase in apoptosis and in expression of α-SMA. Moreover, the levels of urea and creatinine were elevated. Treating animals with MSCs revealed that kidney tissue displayed an improvement in the histological and histochemical changes. Apoptosis and α-SMA expression were decreased, and the levels of urea and creatinine decreased.
  
  
  CONCLUSIONS
  The obtained results demonstrated the potential of MSCs to ameliorate the structure and function of the kidney in rats with anti-Thy1,1 nephritis possibly through the release of paracrine growth factor(s).
authors.affiliation: Department of Zoology, Faculty of Science, Menoufia University, Shebin El-kom, Egypt.
authors.forename: Saber
authors.initials: S
authors.lastname: Sakr
authors.forename: Laila
authors.initials: L
authors.lastname: Rashed
authors.forename: Waheba
authors.initials: W
authors.lastname: Zarouk
authors.forename: Rania
authors.initials: R
authors.lastname: El-Shamy
mesh_terms: D000818:Animals; D005260:Female; D007518:Isoantibodies / Q000633:toxicity*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D009393:Nephritis / Q000139:chemically induced / Q000601:surgery*; D051381:Rats
chemical_list: D007518:Isoantibodies; C035678:anti-Thy antibody
keywords: Anti-Thy1,1; Apoptosis; Mesenchymal stem cells; Nephritis; α-SMA