title: Identification of bacterial infections and clinical manifestation associated with cytomegalovirus in liver transplantation patients.
abstract: BACKGROUND
  Liver transplantation has become the most effective therapy for the treatment of patients with end-stage liver disease. With new immunosuppressive agents the incidence of acute rejection has been significantly reduced, but infection has become a serious problem.
  
  
  OBJECTIVE
  Our objective was to correlate cytomegalovirus (CMV) positivity of antigenemia and polymerase chain reaction (PCR) with clinical manifestations and bacterial infections among patients undergoing liver transplantation.
  
  
  METHODS
  This prospective study included patients monitored for 6 months for early detection of CMV infection. Sample collections were performed at the time of surgery and weekly until the second month followed by fortnightly in the third month, and monthly in the fourth to sixth month. CMV infection was defined by positive antigenemia (>3 cells) or 2 positive PCR tests associated or not with clinical symptoms. The methodology for the diagnosis of bacterial infection was through biochemical tests and the automated VITEK/bioMérieux (identification and antibiogram) using samples of urine and blood cultures. Chi-square test was used for dicotomic variables with significant differences when P < .05.
  
  
  RESULTS
  Sixteen patients (32%) had CMV infections, including 13 (81%) with concomitant infections. Thirty-four patients (68%) did not have CMV infections and 8 of these (24%) had bacterial infection. There was a high correlation with bacterial infections among CMV-positive patients.
  
  
  CONCLUSIONS
  Bacterial infections after liver transplantation were associated with CMV infection.
authors.affiliation: State University of Campinas, Campinas, Brazil.
authors.forename: A
authors.initials: A
authors.lastname: Milan
authors.forename: A M
authors.initials: AM
authors.lastname: Sampaio
authors.forename: A C
authors.initials: AC
authors.lastname: Guardia
authors.forename: C R
authors.initials: CR
authors.lastname: Pavan
authors.forename: P D
authors.initials: PD
authors.lastname: Andrade
authors.forename: S H A
authors.initials: SH
authors.lastname: Bonon
authors.forename: S C B
authors.initials: SC
authors.lastname: Costa
authors.forename: E C
authors.initials: EC
authors.lastname: Ataíde
authors.forename: I F S F
authors.initials: IF
authors.lastname: Boin
authors.forename: R S B
authors.initials: RS
authors.lastname: Stucchi
mesh_terms: D001424:Bacterial Infections / Q000150:complications*; D003587:Cytomegalovirus / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000150:complications*; D006801:Humans; D016031:Liver Transplantation; D016133:Polymerase Chain Reaction