title: A panel of specific monoclonal antibodies directed against various phosphorylated histones H3.
abstract: Modification of histone plays a critical role in the epigenetic regulation of gene expression. However, unlike the widely studied roles of histone methylation or acetylation of histone H3, relatively little is known about the molecular mechanisms involved in translating histone phosphorylation into a specific outcome. The present study reports on the development of antibodies (MAbs) directed against phosphorylated histone H3 (S10, T11, S28, S31, and T32), produced by the hybridization of mouse myeloma cells with lymph node cells from an immunized rat or mouse. The MAbs produced specifically recognize different sites of phosphorylation on histone H3. All of these MAbs are suitable for immunoblotting and immunofluorescence analysis. We believe that these antibodies should significantly facilitate our efforts to investigate epigenetic regulation.
authors.affiliation: Department of Bioengineering, Graduate School of Engineering, Osaka City University, Osaka, Japan.
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Yoshimi
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Ohkawa
authors.forename: Masayuki
authors.initials: M
authors.lastname: Azuma
authors.forename: Taro
authors.initials: T
authors.lastname: Tachibana
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000737:chemistry*; D000918:Antibody Specificity; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006367:HeLa Cells; D006657:Histones / Q000737:chemistry / Q000276:immunology*; D006801:Humans; D006825:Hybridomas; D051379:Mice; D008810:Mice, Inbred C57BL; D010446:Peptide Fragments / Q000737:chemistry / Q000276:immunology; D010750:Phosphoproteins / Q000737:chemistry / Q000276:immunology; D051381:Rats; D011921:Rats, Inbred WKY
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D006657:Histones; D010446:Peptide Fragments; D010750:Phosphoproteins