title: Expression of neutral β-glucosidase from Scytalidium thermophilum in Candida glabrata for ethanol production from alkaline-pretreated rice straw.
abstract: We successfully expressed the neutral β-glucosidase (BGL4) from Scytalidium thermophilum in the thermotolerant yeast Candida glabrata. Compared to the strain expressing Aspergillus acidic β-glucosidase (BGL1), the BGL4-expressing strain showed a higher cellobiose fermentation ability at pH 6.0 and 40°C, leading to a higher ethanol production from alkaline-pretreated rice straw.
authors.affiliation: National Food Research Institute, National Agriculture and Food Research Organization (NARO), 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Wang
authors.forename: Masakazu
authors.initials: M
authors.lastname: Ike
authors.forename: Riki
authors.initials: R
authors.lastname: Shiroma
authors.forename: Ken
authors.initials: K
authors.lastname: Tokuyasu
authors.forename: Yoshikiyo
authors.initials: Y
authors.lastname: Sakakibara
mesh_terms: D001203:Ascomycota / Q000201:enzymology* / Q000235:genetics; D001230:Aspergillus / Q000201:enzymology; D041221:Candida glabrata / Q000235:genetics* / Q000378:metabolism*; D002475:Cellobiose / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D003904:Mitosporic Fungi / Q000201:enzymology / Q000235:genetics; D012275:Oryza; D013696:Temperature; D001617:beta-Glucosidase / Q000235:genetics / Q000378:metabolism*
chemical_list: D002475:Cellobiose; D000431:Ethanol; D001617:beta-Glucosidase
keywords: Bioethanol; Candida glabrata; Cellobiose; Scytalidium thermophilum; β-Glucosidase