title: Treatment of nonhealing diabetic lower extremity ulcers with skin graft and autologous platelet gel: a case series.
abstract: Lower extremity ulcers in diabetic patients are difficult to treat. Recently, the use of human blood platelet-derived components in this indication has been raising interest. In this study, we have evaluated the safety and efficacy of the combination of autologous platelet gel (PG) and skin graft for treating large size recalcitrant ulcers. Eight consecutive diabetic patients aged 25 to 82 with nine nonhealing lower extremity ulcers (median size of 50 cm(2); range 15-150 cm(2)) were treated. Skin ulcer was debrided, and the wound was sprayed after 7 to 10 days with autologous platelet-rich plasma and thrombin. Thin split-thickness skin graft with multiple slits was then applied on the wound bed and fixed with staples or cat-gut sutures. There were no adverse reactions observed during the study. Eight out of 9 skin grafts took well. The interval between skin graft and complete wound healing ranged from 2 to 3 weeks in the 8 successful cases. No ulcer recurrence was noted in those patients during the follow-up period of 2 to 19 months. In this study, the combination of autologous platelet gel and skin grafting has proven beneficial to heal large-size recalcitrant ulcers.
authors.affiliation: Division of Plastic Surgery, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, 11490 Taipei, Taiwan.
authors.forename: Yuan-Sheng
authors.initials: YS
authors.lastname: Tzeng
authors.forename: Shou-Cheng
authors.initials: SC
authors.lastname: Deng
authors.forename: Chih-Hsing
authors.initials: CH
authors.lastname: Wang
authors.forename: Jui-Che
authors.initials: JC
authors.lastname: Tsai
authors.forename: Tim-Mo
authors.initials: TM
authors.lastname: Chen
authors.forename: Thierry
authors.initials: T
authors.lastname: Burnouf
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D048909:Diabetes Complications / Q000188:drug therapy* / Q000473:pathology; D017719:Diabetic Foot / Q000188:drug therapy* / Q000473:pathology; D005260:Female; D016523:Foot Ulcer / Q000188:drug therapy* / Q000473:pathology; D006801:Humans; D035002:Lower Extremity / Q000473:pathology; D008297:Male; D008875:Middle Aged; D053657:Platelet-Rich Plasma; D016038:Skin Transplantation; D013917:Thrombin / Q000008:administration & dosage*; D014945:Wound Healing
chemical_list: D013917:Thrombin