title: Polysaccharide-hair cationic polypeptide nanogels: self-assembly and enzymatic polymerization of amylose primer-modified cholesteryl poly(L-lysine).
abstract: In this study, we prepared a new associating polymer, ChMaPLL, by the substitution of the poly(L-lysine) moiety with oligosaccharide amylose primer and cholesterol. ChMaPLL formed positively charged polypeptide nanogels (~50 nm) via self-assembly in water. The nanogels showed a secondary structural transition to an α-helix structure induced by poly(L-lysine) in response to an increase in pH. Oligosaccharides of the nanogels reacted with the phosphorylase a enzyme. Amylose-conjugated nanogels were obtained by enzymatic polymerization. The elongation of the saccharide chain shielded the positive charge of the nanogels. The multiresponsive polysaccharide-polypeptide hybrid nanogels might prove to be useful in the areas of biotechnology and biomedicine.
authors.affiliation: Department of Materials Processing, Graduate School of Engineering, Tohoku University, Aoba-ku, Sendai, Japan.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Morimoto
authors.forename: Mio
authors.initials: M
authors.lastname: Yamazaki
authors.forename: Junko
authors.initials: J
authors.lastname: Tamada
authors.forename: Kazunari
authors.initials: K
authors.lastname: Akiyoshi
mesh_terms: D000688:Amylose / Q000737:chemistry*; D001704:Biopolymers / Q000737:chemistry*; D002784:Cholesterol / Q000737:chemistry*; D002942:Circular Dichroism; D005456:Fluorescent Dyes; D006197:Hair / Q000737:chemistry*; D006801:Humans; D049329:Nanostructures; D010316:Particle Size; D011107:Polylysine / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: D001704:Biopolymers; D005456:Fluorescent Dyes; D011134:Polysaccharides; D011107:Polylysine; D000688:Amylose; D002784:Cholesterol