title: Cell-free microRNAs: potential biomarkers in need of standardized reporting.
abstract: MicroRNAs are abundantly present and surprisingly stable in multiple biological fluids. These findings have been followed by numerous reverse transcription real-time quantitative PCR (RT-qPCR)-based reports revealing the clinical potential of using microRNA levels in body fluids as a biomarker of disease. Despite a rapidly increasing body of literature, the field has failed to adopt a set of standardized criteria for reporting the methodology used in the quantification of cell-free microRNAs. Not only do many studies based on RT-qPCR fail to address the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) criteria but frequently there is also a distinct lack of detail in descriptions of sample source and RNA isolation. As a direct result, it is often impossible to compare the results of different studies, which in turn, hinders progress in the field. To address this point, we propose a simple set of criteria to be used in conjunction with MIQE to reveal the true potential of cell-free microRNAs as biomarkers.
authors.affiliation: Asbestos Diseases Research Institute, University of Sydney Sydney, NSW, Australia.
authors.forename: Michaela B
authors.initials: MB
authors.lastname: Kirschner
authors.forename: Nico
authors.initials: N
authors.lastname: van Zandwijk
authors.forename: Glen
authors.initials: G
authors.lastname: Reid
keywords: cell-free microRNA; isolation; quantification; reporting; standard