title: Nrf2/ARE is the potential pathway to protect Sprague-Dawley rats against oxidative stress induced by quinocetone.
abstract: 3-methyl-2-quinoxalin benzenevinylketo-1, 4-dioxide (Quinocetone, QCT) is a newly used veterinary drug which has been proven to promote feed efficiency and growth of animals; however, its potential toxicity can't be ignored. Therefore, the present study was aimed to investigate the nephrotoxicity of QCT and the oxidative stress induced by it. Sprague-Dawley rats (SD rats) were randomly divided into four groups with doses of 2400, 800, 50 and 0mg/kg/day with administration of QCT for 4 weeks. Results proved that QCT could induce nephrotoxicity and this phenomenon had dose dependent manner. Simultaneously, this phenomenon was accompanied by intracellular reactive oxygen species (ROS) accumulation, enhanced lipid peroxidation and inhibited antioxidant system, i.e. glutathione S-transferase (GST), glutathione peroxidase (GPx) and glutathione reductase (GSH). Additionally, the higher expression of Nrf2 in QCT treated groups illustrated that QCT-induced oxidative stress would be partly mitigated by the induction of phase II detoxifying enzymes via increasing Nrf2 expression.
authors.affiliation: Department of Nutrition and Food Hygiene, Hubei Key Laboratory of Food Nutrition and Safety, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Yu
authors.forename: Mengjing
authors.initials: M
authors.lastname: Xu
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Yang
authors.forename: Ying
authors.initials: Y
authors.lastname: Rong
authors.forename: Ping
authors.initials: P
authors.lastname: Yao
authors.forename: Hong
authors.initials: H
authors.lastname: Yan
authors.forename: Di
authors.initials: D
authors.lastname: Wang
authors.forename: Liegang
authors.initials: L
authors.lastname: Liu
mesh_terms: D000818:Animals; D061745:Antioxidant Response Elements / Q000187:drug effects* / Q000235:genetics; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000097:blood; D002374:Catalase / Q000097:blood; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000097:blood; D007674:Kidney Diseases / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D007677:Kidney Function Tests; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D051267:NF-E2-Related Factor 2 / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects* / Q000235:genetics; D011810:Quinoxalines / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D051267:NF-E2-Related Factor 2; C495637:Nfe2l2 protein, rat; D011810:Quinoxalines; D017382:Reactive Oxygen Species; C502851:quinocetone; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione