title: TNF-α up-regulates cellular inhibitor of apoptosis protein 2 (c-IAP2) via c-Jun N-terminal kinase (JNK) pathway in nasopharyngeal carcinoma.
abstract: Inhibitor of apoptosis proteins (IAPs) contribute to both tumor progression and tumor metastasis. Here, we show that pro-inflammatory cytokine TNF-α induced the up-regulation of c-IAP2 in the potential metastatic nasopharyngeal carcinoma (NPC) cells in a dose- and time-dependent manner. This up-regulation is tolerant, as the pre-treatment of NPC cells with TNF-α reversed the up-regulation of c-IAP2 induced by TNF-α re-stimulation. TNF-α activated MAKP signals, including ERK, JNK and p38, and NF-κB signal, but only inhibition of JNK signal transduction reversed the induction of c-IAP2, suggesting that JNK signaling contributed to the c-IAP2 induction. The results from in vitro scratch wound-healing assays showed that TNF-α promoted cell invasion, which was reversed by the inhibition of JNK signaling. Taken together, these studies suggested that pro-inflammation cytokine TNF-α may be a promoter for NPC metastasis, and the anti-inflammatory therapy may be of benefit to the prevention of NPC metastasis.
authors.affiliation: Cancer Center, Renmin Hospital of Wuhan University, Wuhan 430060, China.
authors.forename: Qibin
authors.initials: Q
authors.lastname: Song
authors.forename: Guihua
authors.initials: G
authors.lastname: Wang
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Chu
authors.forename: Lina
authors.initials: L
authors.lastname: Zhou
authors.forename: Manli
authors.initials: M
authors.lastname: Jiang
authors.forename: Qing
authors.initials: Q
authors.lastname: He
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.forename: Jian
authors.initials: J
authors.lastname: Qin
authors.forename: Jinyue
authors.initials: J
authors.lastname: Hu
mesh_terms: D002277:Carcinoma; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000096:biosynthesis* / Q000235:genetics; D020935:MAP Kinase Signaling System / Q000187:drug effects; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism*; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology*; D015854:Up-Regulation
chemical_list: D051034:Inhibitor of Apoptosis Proteins; D047888:Receptors, Tumor Necrosis Factor, Type I; D014409:Tumor Necrosis Factor-alpha