title: Detection of Pepino mosaic virus isolates from tomato by one-step reverse transcription loop-mediated isothermal amplification.
abstract: Loop-mediated isothermal amplification (LAMP) of DNA is a novel technique for the amplification of DNA under isothermal conditions. For the first time, we applied this method to develop a simple and sensitive tool for the detection of Pepino mosaic virus (PepMV). PepMV is an emerging pathogen that causes yield and quality losses in tomato crops. Specific RT-LAMP primers for PepMV detection were designed based on triple gene block sequences. The reaction was performed in a single tube at 65 °C for 30 min. The RT-LAMP assay is easy to perform and inexpensive, and it may be applied in the rapid and specific diagnosis of PepMV.
authors.affiliation: Department of Virology and Bacteriology, Institute of Plant Protection-National Research Institute, ul. Wł. Węgorka 20, 60-318, Poznań, Poland, beatahasiow@tlen.pl.
authors.forename: Beata
authors.initials: B
authors.lastname: Hasiów-Jaroszewska
authors.forename: Natasza
authors.initials: N
authors.lastname: Borodynko
mesh_terms: D005838:Genotype; D018551:Solanum lycopersicum / Q000821:virology*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D010935:Plant Diseases / Q000821:virology; D010942:Plant Viruses / Q000302:isolation & purification*; D048348:Reverse Transcription; D012680:Sensitivity and Specificity