title: Primary pathogenicity analysis of a Chinese Entamoeba histolytica isolate.
abstract: This study is the first to isolate an Entamoeba histolytica strain from Chinese amoebic patients and to conduct a detailed examination of its virulence. A fecal sample that contains cysts of E. histolytica was obtained from Guangxi province. The sample was cultured axenically and then cloned by limiting dilution, and named as XLAC. In vitro and in vivo tests were conducted to evaluate the virulence of the Entamoeba isolate. The E. histolytica strain XLAC was successfully cloned and cultured axenically. DNA regions that contain hexokinase, glucose-6-phosphate isomerase, phosphoglucomutase, and heavy subunit of lectin genes were amplified by PCR. The PCR products were then sequenced. Virulence analysis suggested that the XLAC strain was similar to the HM1:IMSS strain at the genetic level. In vitro and in vivo tests also implicated these strains to be similar. These findings may be attributed to the low expression levels of pathogenic genes obtained through realtime PCR. The XLAC strain restored its virulence after it was injected into hamster liver. This study may be a good model for studying virulence changes in E. histolytica.
authors.affiliation: Department of Medical Microbiology and Parasitology, Shanghai Medical College of Fudan University, Shanghai 200032, China.
authors.forename: Muxia
authors.initials: M
authors.lastname: Luo
authors.forename: Meng
authors.initials: M
authors.lastname: Feng
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Min
authors.forename: Xueping
authors.initials: X
authors.lastname: Li
authors.forename: Junlong
authors.initials: J
authors.lastname: Cai
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tachibana
authors.forename: Xunjia
authors.initials: X
authors.lastname: Cheng
mesh_terms: D000818:Animals; D017209:Apoptosis; D002448:Cell Adhesion; D002681:China; D006224:Cricetinae; D004748:Entamoeba histolytica / Q000302:isolation & purification* / Q000472:pathogenicity*; D004749:Entamoebiasis / Q000469:parasitology / Q000473:pathology; D004912:Erythrocytes / Q000378:metabolism / Q000382:microbiology / Q000469:parasitology; D005786:Gene Expression Regulation; D006801:Humans; D019169:Jurkat Cells; D037102:Lectins / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000469:parasitology / Q000473:pathology; D008297:Male; D010587:Phagocytosis; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D053058:Trophozoites / Q000502:physiology
chemical_list: D037102:Lectins; D021122:Protein Subunits