title: Serotype IV and invasive group B Streptococcus disease in neonates, Minnesota, USA, 2000-2010.
abstract: Group B Streptococcus (GBS) is a major cause of invasive disease in neonates in the United States. Surveillance of invasive GBS disease in Minnesota, USA, during 2000-2010 yielded 449 isolates from 449 infants; 257 had early-onset (EO) disease (by age 6 days) and 192 late-onset (LO) disease (180 at age 7-89 days, 12 at age 90-180 days). Isolates were characterized by capsular polysaccharide serotype and surface-protein profile; types III and Ia predominated. However, because previously uncommon serotype IV constitutes 5/31 EO isolates in 2010, twelve type IV isolates collected during 2000-2010 were studied further. By pulsed-field gel electrophoresis, they were classified into 3 profiles; by multilocus sequence typing, representative isolates included new sequence type 468. Resistance to clindamycin or erythromycin was detected in 4/5 serotype IV isolates. Emergence of serotype IV GBS in Minnesota highlights the need for serotype prevalence monitoring to detect trends that could affect prevention strategies.
authors.affiliation: Department of Laboratory Medicine, University of Minnesota Medical School, University of Minnesota Hospital, Minneapolis, Minnesota 55455, USA. ferri002@umn.edu
authors.forename: Patricia
authors.initials: P
authors.lastname: Ferrieri
authors.forename: Ruth
authors.initials: R
authors.lastname: Lynfield
authors.forename: Roberta
authors.initials: R
authors.lastname: Creti
authors.forename: Aurea E
authors.initials: AE
authors.lastname: Flores
mesh_terms: D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D002981:Clindamycin / Q000627:therapeutic use; D004269:DNA, Bacterial / Q000097:blood / Q000145:classification / Q000235:genetics*; D024881:Drug Resistance, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D004917:Erythromycin / Q000627:therapeutic use; D005838:Genotype; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008910:Minnesota / Q000453:epidemiology; D058885:Multilocus Sequence Typing; D012189:Retrospective Studies; D012703:Serotyping; D013290:Streptococcal Infections / Q000097:blood / Q000188:drug therapy / Q000453:epidemiology / Q000382:microbiology*; D013292:Streptococcus agalactiae / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D002981:Clindamycin; D004917:Erythromycin
keywords: CPS; GBS; Minnesota; United States; antimicrobial resistance; bacteria; capsular polysaccharide; clindamycin resistance; early-onset GBS disease; group B Streptococcus; invasive GBS disease; late-onset GBS disease; neonatal GBS disease; neonates; prophylaxis; serotype; serotype IV; streptococci