title: CuS nanoparticles as a mimic peroxidase for colorimetric estimation of human blood glucose level.
abstract: CuS nanoparticles (NPs) was synthesized through a simple and green method using water soluble precursor complex [CuL2(H2O)2]Cl2 (L=pyridine 2-carboxamide) and was characterized by X-ray diffraction analysis (XRD), transmission electron microscopy (TEM) and UV-Vis spectroscopic techniques. The as-prepared CuS NPs (covellite) was demonstrated to possess intrinsic peroxidase-like activity using 3,3',5,5'-tetramethylbenzidine (TMB), as a peroxidase substrate, in presence of H2O2 which show good affinity towards both TMB and H2O2. Using this TMB-H2O2 catalyzed color reaction; the CuS NPs was exploited as a new type of biosensor for detection and estimation of glucose through a simple, cheap and selective colorimetric method in a linear range from 2 to 1800 μM with a detection limit of 0.12 μM. On the basis of the developed reaction process, we can easily monitor human blood glucose level.
authors.affiliation: Department of Chemistry, Bengal Engineering and Science University, Shibpur, Howrah 711103, West Bengal, India.
authors.forename: Amit Kumar
authors.initials: AK
authors.lastname: Dutta
authors.forename: Sudipto
authors.initials: S
authors.lastname: Das
authors.forename: Suvendu
authors.initials: S
authors.lastname: Samanta
authors.forename: Partha Kumar
authors.initials: PK
authors.lastname: Samanta
authors.forename: Bibhutosh
authors.initials: B
authors.lastname: Adhikary
authors.forename: Papu
authors.initials: P
authors.lastname: Biswas
mesh_terms: D001560:Benzidines / Q000378:metabolism; D001786:Blood Glucose / Q000032:analysis*; D002384:Catalysis; D003124:Colorimetry / Q000379:methods*; D003300:Copper / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009195:Peroxidase / Q000378:metabolism
chemical_list: D001560:Benzidines; D001786:Blood Glucose; C021758:3,3',5,5'-tetramethylbenzidine; D003300:Copper; D006861:Hydrogen Peroxide; D009195:Peroxidase; C017846:cupric sulfide