title: [Bacterial diversity within different sections of summer sea-ice samples from the Prydz Bay, Antarctica].
abstract: OBJECTIVE
  In order to assess bacterial abundance and diversity within three different sections of summer sea-ice samples collected from the Prydz Bay, Antarctica.
  
  
  METHODS
  Fluorescence in situ hybridization was applied to determine the proportions of Bacteria in sea-ice. Bacterial community composition within sea ice was analyzed by 16S rRNA gene clone library construction. Correlation analysis was performed between the physicochemical parameters and the bacterial diversity and abundance within sea ice.
  
  
  RESULTS
  The result of fluorescence in situ hybridization shows that bacteria were abundant in the bottom section, and the concentration of total organic carbon, total organic nitrogen and phosphate may be the main factors for bacterial abundance. In bacterial 16S rRNA gene libraries of sea-ice, nearly complete 16S rRNA gene sequences were grouped into three distinct lineages of Bacteria (gamma-Proteobacteria, alpha-Proteobacteria and Bacteroidetes). Most clone sequences were related to cultured bacterial isolates from the marine environment, arctic and Antarctic sea-ice with high similarity. The member of Bacteroidetes was not detected in the bottom section of sea-ice. The bacterial communities within sea-ice were little heterogeneous at the genus-level between different sections, and the concentration of NH4+ may cause this distribution.
  
  
  CONCLUSIONS
  The number of bacteria was abundant in the bottom section of sea-ice. Gamma-proteobacteria was the dominant bacterial lineage in sea-ice.
authors.affiliation: SOA Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai 200136, China. jifeima007@163.com
authors.forename: Jifei
authors.initials: J
authors.lastname: Ma
authors.forename: Zongjun
authors.initials: Z
authors.lastname: Du
authors.forename: Wei
authors.initials: W
authors.lastname: Luo
authors.forename: Yong
authors.initials: Y
authors.lastname: Yu
authors.forename: Yixin
authors.initials: Y
authors.lastname: Zeng
authors.forename: Bo
authors.initials: B
authors.lastname: Chen
authors.forename: Huirong
authors.initials: H
authors.lastname: Li
mesh_terms: D000864:Antarctic Regions; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D044822:Biodiversity; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D017753:Ecosystem; D046448:Ice Cover / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012621:Seasons
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal