title: Monitoring of Ochratoxin A biosynthesis related genes in Penicillium verrucosum by differential display reverse transcription PCR (DDRT-PCR) experiments.
abstract: Differential display reverse transcription polymerase chain reaction experiments were performed to monitore Ochratoxin A biosynthetic genes inPenicillium verrucosum BFE487. A stringent prerequisite for the performance of this type of experiments is the knowledge of the differential expression of OTA genes. Differentially expressed total RNA from permissive/restrictive growth conditions and alternatively OTA(+) wild type/OTA mutant strains have been isolated, reverse transcribed and used as a template in DDRT-PCR experiments. About 70 differentially expressed cDNA fragments could be isolated, cloned and sequenced.
authors.affiliation: Federal Research Center for Nutrition Institute of Hygiene, Toxicology Haid-und-Neu-Str. 9, D-76131, Karlsruhe, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Färber
authors.forename: R
authors.initials: R
authors.lastname: Geisen