title: Tissue sample preparation for biomarker discovery.
abstract: Global protein expression studies, an approach known as "proteomics," can offer important clues for understanding tumor biology that cannot be obtained by other approaches. Proteomic studies have provided protein expression profiles of tumors that can be used to develop novel diagnostic and therapeutic biomarkers. In this chapter, we describe the strategy and design of proteomic studies, as well as the protocols for tissue sample collection and preparation for biomarker discovery, especially tumor biomarkers, followed by a few examples of our recent proteomic studies.
authors.affiliation: Department of Orthopedic Surgery, Juntendo University School of Medicine, Tokyo, Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Suehara
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kubota
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Saito
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis* / Q000737:chemistry; D020869:Gene Expression Profiling; D006801:Humans; D009369:Neoplasms / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D013048:Specimen Handling
chemical_list: D014408:Biomarkers, Tumor