title: Molecular detection and phylogenetic analysis of Anaplasma bovis from Haemaphysalis longicornis feeding on grazing cattle in Korea.
abstract: Ticks are vectors of various pathogens that affect humans and animals throughout the world. Anaplasma bovis is one of the most important tick-borne pathogens that cause cattle diseases but there is still very little information available about this agent in Korea. In the present study, 535 Haemaphysalis longicornis tick pools were analyzed from grazing cattle in five Korean provinces. A. bovis was detected in 50 (9.3%) of 535 tick pools using 16S rRNA-based PCR. A. bovis infections were detected for the first time in ticks feeding on cattle in Chungbuk, Geongbuk, and Jeonbuk provinces in Korea. The 50 positive PCR products were sequenced successfully and compared with sequences in GenBank. Phylogenetic analysis of the Korean isolates classified them into four genotypes with nucleotide sequence identities of 99.4-100%. Two of the four genotypes had high similarity (99.8-100%) with known sequences. The other two genotypes have never been identified.
authors.affiliation: Parasitology and Insect Disease Research Laboratory, Animal, Plant and Fisheries Quarantine and Inspection Agency, 480 Anyang 6 dong, Anyang 420-480, Republic of Korea. dt.thanhhuong@ibt.ac.vn
authors.forename: Huong Thi Thanh
authors.initials: HT
authors.lastname: Doan
authors.forename: Jin Hyeong
authors.initials: JH
authors.lastname: Noh
authors.forename: Se Eun
authors.initials: SE
authors.lastname: Choe
authors.forename: Mi Sun
authors.initials: MS
authors.lastname: Yoo
authors.forename: Young Ha
authors.initials: YH
authors.lastname: Kim
authors.forename: Kondreddy Eswar
authors.initials: KE
authors.lastname: Reddy
authors.forename: Dong Van
authors.initials: DV
authors.lastname: Quyen
authors.forename: Lien Thi Kim
authors.initials: LT
authors.lastname: Nguyen
authors.forename: Thuy Thi Dieu
authors.initials: TT
authors.lastname: Nguyen
authors.forename: Chang Hee
authors.initials: CH
authors.lastname: Kweon
authors.forename: Suk Chan
authors.initials: SC
authors.lastname: Jung
authors.forename: Ki Yoon
authors.initials: KY
authors.lastname: Chang
authors.forename: Seung Won
authors.initials: SW
authors.lastname: Kang
mesh_terms: D000709:Anaplasma / Q000235:genetics* / Q000302:isolation & purification*; D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000469:parasitology*; D010802:Phylogeny; D056910:Republic of Korea / Q000453:epidemiology; D013984:Tick Infestations / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D013987:Ticks / Q000382:microbiology*
keywords: Anaplasma bovis; Grazing cattle; Haemaphysalis longicornis; PCR