title: Green improved processes to extract bioactive phenolic compounds from brown macroalgae using Sargassum muticum as model.
abstract: A comparative study between "alternative" extraction processes such as centrifugal partition extraction (CPE), supercritical fluid extraction (SFE) and pressurized liquid extraction (PLE) and classical solid/liquid used in the laboratory are currently focusing on the efficiency (selectivity and productivity) to obtain bioactive phenolic compounds from the phaeophyte Sargassum muticum model. The choice of the best process was based on several measurements: (i) the total phenolic content measured by the colorimetric Folin-Ciocalteu assay, (ii) radical scavenger and antioxidant activities assessed by the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay, and the β-carotene bleaching method and finally (iii) the method productivity. Irrespective of the solvent used in the processes, alternative methods are always sharply more effective than classical ones. With the exception of SFE which does not allow extracting the totality of the active phenolic compounds, two of the other extraction methods were particularly promising. First, CPE afforded the most important yields in concentrated phenolic compounds (PC) (22.90±0.65% DW) also displaying the best activities (0.52±0.02 and 0.58±0.19 mg/mL for IC50 and AAC700, respectively). Secondly, PLE using an EtOH:water mixture 75:25 (v/v) allowed a good PC extraction (10.18±0.25% DW) with huge efficiency. Despite a lesser activity of the extracts (0.77±0.01 and 1.59±0.15 mg/mL for IC50 and AAC700, respectively) PLE is a green process and potentially complies European norms requirements for the prospective valorization of phenolic compounds from S. muticum in Brittany.
authors.affiliation: LEMAR UMR CNRS UBO IRD IFREMER 6539, Université de Bretagne Occidentale (UBO), Institut Universitaire Européen de la Mer (IUEM), Technopôle Brest-Iroise, Rue Dumont d'Urville, 29280 Plouzané, France. anaelle.tanniou@live.fr
authors.forename: Tanniou
authors.initials: T
authors.lastname: Anaëlle
authors.forename: Esteban
authors.initials: E
authors.lastname: Serrano Leon
authors.forename: Vandanjon
authors.initials: V
authors.lastname: Laurent
authors.forename: Ibanez
authors.initials: I
authors.lastname: Elena
authors.forename: Jose A
authors.initials: JA
authors.lastname: Mendiola
authors.forename: Cerantola
authors.initials: C
authors.lastname: Stéphane
authors.forename: Kervarec
authors.initials: K
authors.lastname: Nelly
authors.forename: La Barre
authors.initials: LB
authors.lastname: Stéphane
authors.forename: Marchal
authors.initials: M
authors.lastname: Luc
authors.forename: Stiger-Pouvreau
authors.initials: SP
authors.lastname: Valérie
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification*; D001713:Biphenyl Compounds / Q000737:chemistry; D055772:Green Chemistry Technology; D009682:Magnetic Resonance Spectroscopy; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification*; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry; D044647:Sargassum / Q000737:chemistry*; D019207:beta Carotene / Q000737:chemistry
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D019207:beta Carotene; C004931:1,1-diphenyl-2-picrylhydrazyl