title: Nukbone® promotes proliferation and osteoblastic differentiation of mesenchymal stem cells from human amniotic membrane.
abstract: Bovine bone matrix Nukbone® (NKB) is an osseous tissue-engineering biomaterial that retains its mineral and organic phases and its natural bone topography and has been used as a xenoimplant for bone regeneration in clinics. There are not studies regarding its influence of the NKB in the behavior of cells during the repairing processes. The aim of this research is to demonstrate that NKB has an osteoinductive effect in human mesenchymal stem cells from amniotic membrane (AM-hMSCs). Results indicated that NKB favors the AM-hMSCs adhesion and proliferation up to 7 days in culture as shown by the scanning electron microscopy and proliferation measures using an alamarBlue assay. Furthermore, as demonstrated by reverse transcriptase polymerase chain reaction, it was detected that two gene expression markers of osteoblastic differentiation: the core binding factor and osteocalcin were higher for AM-hMSCs co-cultured with NKB in comparison with cultivated cells in absence of the biomaterial. As the results indicate, NKB possess the capability for inducing successfully the osteoblastic differentiation of AM-hMSC, so that, NKB is an excellent xenoimplant option for repairing bone tissue defects.
authors.affiliation: Depto Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City 04510, Mexico.
authors.forename: Nayeli
authors.initials: N
authors.lastname: Rodríguez-Fuentes
authors.forename: Ana G
authors.initials: AG
authors.lastname: Rodríguez-Hernández
authors.forename: Juana
authors.initials: J
authors.lastname: Enríquez-Jiménez
authors.forename: Luz E
authors.initials: LE
authors.lastname: Alcántara-Quintana
authors.forename: Lizeth
authors.initials: L
authors.lastname: Fuentes-Mera
authors.forename: María C
authors.initials: MC
authors.lastname: Piña-Barba
authors.forename: Armando
authors.initials: A
authors.lastname: Zepeda-Rodríguez
authors.forename: Javier R
authors.initials: JR
authors.lastname: Ambrosio
mesh_terms: D000650:Amnion; D000818:Animals; D002417:Cattle; D002454:Cell Differentiation; D049109:Cell Proliferation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010006:Osteoblasts / Q000166:cytology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D054457:Tissue Scaffolds