title: [Experimental study on Yougui recipe in preventing osteolysis surrounding artificial prosthesis].
abstract: OBJECTIVE
  To explore effects of Yougui recipe (see text) and salmon calcitonin acetate in preventing osteolysis surrounding artificial prosthesis.
  
  
  METHODS
  Thirty-two SD male rats with weighted (250 +/- 20) g, aged 8 weeks, were randomly divided into four groups: blank group, model group, salmon calcitonin acetate group and Yougui recipe (see text) group, and 8 rats in each group. Blank group did not undergo any process, other 24 rats underwent anesthesia by chloral hydrate, their knee joints were exposed through medial patellar side,drilling from fermoral condyle nest to marrow cavity,high density of polythlene particles were injected into hole, titanium nail were put into, bone wax closed the window, then suturing step by step. After the molding, saline were used to gavaged in blank group and model group, Yougui recipe (see text) for Yougui recipe (see text) group, salmon calcitonin maximus injection for calcitonin group. After 10 weeks' mediation, rats were executed, and arterial blood and bilateral femoral organization were collected to biochemical, imaging morphology, tissue pathology and molecular biology detection.
  
  
  RESULTS
  The key gene expression of activiting osteoclast were inhibited in Yougui recipe (see text) group and calcitonin group. The level of OPG, Ca, ALP in Yougui recipe group were higher than calcitonin group (P<0.01); the content of RANKL were lower (P<0.01). There were no significance meaning in RANK, Trap5b, P between two groups.
  
  
  CONCLUSIONS
  Both of Yougui recipe (see text) and calcitonin can slow and treat surrounding osteolysis of artificial joint prosthesis, and Yougui recipe (see text) has better effect in promoting bone formation. The effect of Yougui recipe (see text) in promoting bone formation, inhibiting osteoclasts to provide a new method to treating surrounding osteolysis of artificial joint prosthesis.
authors.affiliation: Zhejiang Province Chinese Medicine Hospital, Hangzhou 310006, Zhejiang, China.
authors.forename: Yun-Huo
authors.initials: YH
authors.lastname: Cai
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
authors.forename: Qiang
authors.initials: Q
authors.lastname: Mao
authors.forename: Hang-Xing
authors.initials: HX
authors.lastname: Bao
authors.forename: Jin-Fa
authors.initials: JF
authors.lastname: Wang
authors.forename: Miao-Feng
authors.initials: MF
authors.lastname: Hu
authors.forename: Lu-Wei
authors.initials: LW
authors.lastname: Xiao
authors.forename: Pei-Jian
authors.initials: PJ
authors.lastname: Tong
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D007375:Interleukin-1 / Q000235:genetics; D015850:Interleukin-6 / Q000235:genetics; D008297:Male; D010014:Osteolysis / Q000209:etiology* / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D019736:Prostheses and Implants / Q000009:adverse effects*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014057:Tomography, X-Ray Computed; D025262:Vacuolar Proton-Translocating ATPases / Q000235:genetics
chemical_list: D015415:Biomarkers; D004365:Drugs, Chinese Herbal; D007375:Interleukin-1; D015850:Interleukin-6; C552041:yougui; D025262:Vacuolar Proton-Translocating ATPases