title: A monoclonal antibody against transmissible gastroenteritis virus generated via immunization of a DNA plasmid bearing TGEV S1 gene.
abstract: Transmissible gastroenteritis virus (TGEV) is a member of the coronaviruses. The viral spike (S) protein of TGEV mediates interaction between TGEV and its susceptible cells. Herein, DNA plasmid bearing TGEV S1 gene (the N terminal half of TGEV S gene) was used to immunize BALB/c mice followed by generation of a monoclonal antibody (MAb) using the hybridoma technique. The generated MAb (1H4) was identified by ELISA. Immunofluorescence assays showed that MAb 1H4 was able to detect infection of cells with TGEV. The MAb 1H4 distinguished TGEV from other control viruses. Additionally, although the type of MAb 1H4 was IgM, it could reduce cell infection by TGEV in a dose-dependent manner.
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Zhao
authors.forename: Jiayi
authors.initials: J
authors.lastname: Zhu
authors.forename: Weijuan
authors.initials: W
authors.lastname: Zhu
authors.forename: Xunliang
authors.initials: X
authors.lastname: Li
authors.forename: Ye
authors.initials: Y
authors.lastname: Tao
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Lv
authors.forename: Xue
authors.initials: X
authors.lastname: Wang
authors.forename: Jiechao
authors.initials: J
authors.lastname: Yin
authors.forename: Cheng
authors.initials: C
authors.lastname: He
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Ren
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D006825:Hybridomas / Q000276:immunology; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000235:genetics; D064370:Spike Glycoprotein, Coronavirus; D005760:Transmissible gastroenteritis virus / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D008562:Membrane Glycoproteins; D064370:Spike Glycoprotein, Coronavirus; D014759:Viral Envelope Proteins; C578557:spike glycoprotein, SARS-CoV; C578553:spike protein, mouse hepatitis virus