title: An anti-human CD13 monoclonal antibody that suppresses the suppressive function of Treg cells.
abstract: CD13 (CD13/aminopeptidase N, APN, or CD13/APN) is a widely expressed type II membrane-bound metalloprotease. It is often overexpressed on cancer cells and expressed on CD4(+)CD25(hi) Treg cell subpopulation with higher suppressive ability. It has been determined to be a promising target in cancer diagnosis and therapy. In this study, a functional anti-human CD13 monoclonal antibody, MAb 9E4, was obtained and the specificity of this MAb was verified by flow cytometry. This MAb effectively recognized the CD13 molecule expressed on a series of malignant cell lines. Furthermore, we demonstrated that MAb 9E4 suppresses the suppressive function of Treg cells. This functional anti-human CD13 MAb provides a valuable tool for further study targeting the CD13 positive Treg cells.
authors.affiliation: General Surgery Department, The Second Affiliated Hospital, Soochow University, Suzhou, Jiangsu Province, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Ge
authors.forename: Songwen
authors.initials: S
authors.lastname: Ju
authors.forename: Peng
authors.initials: P
authors.lastname: Yang
authors.forename: Yongfei
authors.initials: Y
authors.lastname: Tan
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Ge
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Cao
authors.forename: Yanpin
authors.initials: Y
authors.lastname: Zhao
authors.forename: Haorong
authors.initials: H
authors.lastname: Wu
authors.forename: Songguang
authors.initials: S
authors.lastname: Ju
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000494:pharmacology; D061067:Antibodies, Monoclonal, Humanized; D060890:B7-H1 Antigen / Q000378:metabolism; D018826:CD13 Antigens / Q000276:immunology*; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology*; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects*; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D061067:Antibodies, Monoclonal, Humanized; D060890:B7-H1 Antigen; D016212:Transforming Growth Factor beta; C584259:monoclonal antibody 9E4; D016753:Interleukin-10; D018826:CD13 Antigens