title: Coagulation activity and white thrombus formation in the microminipig.
abstract: Swine are becoming increasingly attractive as animal models for clinical research and the recently developed Microminipig (MMPig) has emerged as a possible experimental animal model. In this study, we demonstrated age-dependent changes in hematological parameters and coagulation activity in healthy MMPigs (58 male and 67 females, aged 0-34 months), and investigated white thrombus formation (WTF) using an in vitro microchip flow-chamber system (four males and four females, aged 22-23 months). There was no clear sex or age-dependent differences in any hematological parameters. While activated partial thromboplastin time (APTT) was shorter than prothrombin time (PT), with APTT:PT of 0.88:1, microchip flow-chamber system analysis showed that WTF time was shorter than that in humans, suggesting a possible thrombotic tendency in the MMPig. These results could be useful to life science researchers in the use of the MMPig as an experimental model animal for thrombus formation.
authors.affiliation: Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1, Sakuragaoka, Kagoshima, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Miura
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kawaguchi
authors.forename: Tomoka
authors.initials: T
authors.lastname: Nagasato
authors.forename: Tomonobu
authors.initials: T
authors.lastname: Yamada
authors.forename: Takashi
authors.initials: T
authors.lastname: Ito
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Izumi
authors.forename: Hisayo
authors.initials: H
authors.lastname: Shameshima
authors.forename: Noriaki
authors.initials: N
authors.lastname: Miyoshi
authors.forename: Akihide
authors.initials: A
authors.lastname: Tanimoto
authors.forename: Ikuro
authors.initials: I
authors.lastname: Maruyama
mesh_terms: D000818:Animals; D001777:Blood Coagulation / Q000502:physiology*; D001780:Blood Coagulation Tests; D004909:Erythrocyte Indices; D005260:Female; D008297:Male; D013552:Swine / Q000097:blood / Q000502:physiology*; D013927:Thrombosis