title: Antiviral activity of Bacillus sp. isolated from the marine sponge Petromica citrina against bovine viral diarrhea virus, a surrogate model of the hepatitis C virus.
abstract: The Hepatitis C virus causes chronic infections in humans, which can develop to liver cirrhosis and hepatocellular carcinoma. The Bovine viral diarrhea virus is used as a surrogate model for antiviral assays for the HCV. From marine invertebrates and microorganisms isolated from them, extracts were prepared for assessment of their possible antiviral activity. Of the 128 tested, 2 were considered active and 1 was considered promising. The best result was obtained from the extracts produced from the Bacillus sp. isolated from the sponge Petromica citrina. The extracts 555 (500 µg/mL, SI>18) and 584 (150 µg/mL, SI 27) showed a percentage of protection of 98% against BVDV, and the extract 616, 90% of protection. All of them showed activity during the viral adsorption. Thus, various substances are active on these studied organisms and may lead to the development of drugs which ensure an alternative therapy for the treatment of hepatitis C.
authors.affiliation: Laboratório de Virologia, Departamento de Genética Evolução e Bioagentes, Instituto de Biologia/Universidade Estadual de Campinas-UNICAMP, Cx. Postal 6109, CEP 13083-970 , Campinas/SP/Brazil. jusantiago_farmacia@yahoo.com.br
authors.forename: Juliana Cristina Santiago
authors.initials: JC
authors.lastname: Bastos
authors.forename: Luciana Konecny
authors.initials: LK
authors.lastname: Kohn
authors.forename: Fabiana
authors.initials: F
authors.lastname: Fantinatti-Garboggini
authors.forename: Marina Aiello
authors.initials: MA
authors.lastname: Padilla
authors.forename: Eduardo Furtado
authors.initials: EF
authors.lastname: Flores
authors.forename: Bárbara Pereira
authors.initials: BP
authors.lastname: da Silva
authors.forename: Cláudia Beatriz Afonso
authors.initials: CB
authors.lastname: de Menezes
authors.forename: Clarice Weis
authors.initials: CW
authors.lastname: Arns
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001407:Bacillus / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification; D002417:Cattle; D002460:Cell Line; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D030241:Diarrhea Virus 1, Bovine Viral / Q000187:drug effects* / Q000502:physiology; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D011161:Porifera / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D053585:Virus Attachment / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D004269:DNA, Bacterial