title: Preparation and electrochemical behavior of L-glutamate electrochemical biosensor.
abstract: Using the nano-porous pseudo carbon paste electrode (Nano-PPCPE) as the working electrode, mixing L-glutamate oxidase (L-GLOD), catalase (Cat) and bovine serum albumin (BSA) with phosphate buffer (PB, pH = 7.4), followed by cross-linking with glutaraldehyde, a novel L-glutamate electrochemical biosensor was successfully formed. It was demonstrated that the modified nano-PPCPE exhibits a high selectivity and sensitivity in comparison with the modified CPE. The L-glutamate biosensor showed a linear range from 5 x 10(-7) M to 1 x 10(-5) M with the detection limit of 2.5 x 10(-7) M.
authors.affiliation: Hunan Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou 412007, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Deng
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Liming
authors.initials: L
authors.lastname: Zhang
authors.forename: Zhuoxuan
authors.initials: Z
authors.lastname: Lu
authors.forename: Song
authors.initials: S
authors.lastname: Li
authors.forename: Lijian
authors.initials: L
authors.lastname: Xu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D004566:Electrodes; D018698:Glutamic Acid / Q000032:analysis*; D053758:Nanoparticles / Q000737:chemistry; D016062:Porosity
chemical_list: D018698:Glutamic Acid