title: Cytidine 5'-diphosphocholine ameliorates hyperoxic lung injury in a neonatal rat model.
abstract: BACKGROUND
  Bronchopulmonary dysplasia (BPD) is an important cause of morbidity. The aim of this study was to evaluate the preventive effect of cytidine 5'-diphosphocholine (CDP-choline) treatment on hyperoxic lung injury in a neonatal rat model.
  
  
  METHODS
  A total of 30 newborn pups were divided into control, hyperoxia, and hyperoxia + CDP-choline groups. After birth, pups in the control group were kept in room air and received saline injections, whereas those in hyperoxia and hyperoxia + CDP-choline groups were exposed to 95% O₂ and received daily injections of saline and CDP-choline throughout postnatal day 10, respectively. Histopathological scoring, radial alveolar count, lamellar body membrane protein expression, fibrosis, proinflammatory cytokine levels, lung tissue and bronchoalveolar lavage (BAL) fluid phospholipid content, and apoptosis were evaluated.
  
  
  RESULTS
  Hyperoxia-induced severe lung damage was reduced significantly by CDP-choline treatment. Radial alveolar count and lamellar body membrane protein expression were significantly recovered, and the number of terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling-positive cells, active caspase-3 expression, and tissue proinflammatory cytokine levels were decreased by CDP-choline administration. Lung tissue and BAL phospholipid contents showed significant increases after CDP-choline administration.
  
  
  CONCLUSIONS
  These data show that CDP-choline ameliorates hyperoxic lung injury in a neonatal rat model. It may therefore be suggested that CDP-choline may be a novel therapeutic option for the prevention of BPD.
authors.affiliation: Division of Neonatology, Department of Pediatrics, Gulhane Military Medical Academy, Ankara, Turkey. drmerih@yahoo.com
authors.forename: Merih
authors.initials: M
authors.lastname: Cetinkaya
authors.forename: Mehmet
authors.initials: M
authors.lastname: Cansev
authors.forename: Ilker M
authors.initials: IM
authors.lastname: Kafa
authors.forename: Cuneyt
authors.initials: C
authors.lastname: Tayman
authors.forename: Ferhat
authors.initials: F
authors.lastname: Cekmez
authors.forename: Fuat Emre
authors.initials: FE
authors.lastname: Canpolat
authors.forename: Turan
authors.initials: T
authors.lastname: Tunc
authors.forename: S Umit
authors.initials: SU
authors.lastname: Sarici
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D003566:Cytidine Diphosphate Choline / Q000494:pharmacology / Q000627:therapeutic use*; D004195:Disease Models, Animal; D018496:Hyperoxia / Q000188:drug therapy*; D055370:Lung Injury / Q000188:drug therapy*; D051381:Rats
chemical_list: D003566:Cytidine Diphosphate Choline