title: Stress and death of cnidarian host cells play a role in cnidarian bleaching.
abstract: Coral bleaching occurs when there is a breakdown of the symbiosis between cnidarian hosts and resident Symbiodinium spp. Multiple mechanisms for the bleaching process have been identified, including apoptosis and autophagy, and most previous work has focused on the Symbiodinium cell as the initiator of the bleaching cascade. In this work we show that it is possible for host cells to initiate apoptosis that can contribute to death of the Symbiodinium cell. First we found that colchicine, which results in apoptosis in other animals, causes cell death in the model anemone Aiptasia sp. but not in cultured Symbiodinium CCMP-830 cells or in cells freshly isolated from host Aiptasia (at least within the time frame of our study). In contrast, when symbiotic Aiptasia were incubated in colchicine, cell death in the resident Symbiodinium cells was observed, suggesting a host effect on symbiont mortality. Using live-cell confocal imaging of macerated symbiotic host cell isolates, we identified a pattern where the initiation of host cell death was followed by mortality of the resident Symbiodinium cells. This same pattern was observed in symbiotic host cells that were subjected to temperature stress. This research suggests that mortality of symbionts during temperature-induced bleaching can be initiated in part by host cell apoptosis.
authors.affiliation: Department of Zoology, Oregon State University, Corvallis, OR 97331-2914, USA. paxtonca@gmail.com
authors.forename: Camille W
authors.initials: CW
authors.lastname: Paxton
authors.forename: Simon K
authors.initials: SK
authors.lastname: Davy
authors.forename: Virginia M
authors.initials: VM
authors.lastname: Weis
mesh_terms: D000818:Animals; D020169:Caspases / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects; D002469:Cell Separation; D003063:Cnidaria / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D003078:Colchicine / Q000494:pharmacology; D004141:Dinoflagellida / Q000187:drug effects / Q000502:physiology*; D018869:Heat-Shock Response / Q000187:drug effects; D008954:Models, Biological; D009930:Organic Chemicals / Q000378:metabolism; D012615:Sea Anemones / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D013312:Stress, Physiological / Q000187:drug effects; D013559:Symbiosis / Q000187:drug effects; D013696:Temperature; D013997:Time Factors
chemical_list: D009930:Organic Chemicals; C402795:SYTOX Green; D020169:Caspases; D003078:Colchicine
keywords: Aiptasia; Cnidaria; Symbiodinium; apoptosis; coral bleaching; innate immunity; symbiosis