title: Worldwide genealogy of Entamoeba histolytica: an overview to understand haplotype distribution and infection outcome.
abstract: Although Entamoeba histolytica is one of the most prevalent intestinal parasites, how the different strains of this species are distributed all over the world and how different genotypes are associated with the infection outcome are yet to be fully understood. Recently, the use of a number of molecular markers has made the characterization of several genotypes in those regions with high incidence of amoebiasis possible. This work proposes the first genealogy of E. histolytica, with an haplotype network based on two tRNA gene-linked array of Short Tandem Repeats (STRs) reported until today, and 47 sequences from 39 new isolates of Mexican Amoebic Liver Abscesses (ALA) samples. One hundred and three sequences were obtained from D-A locus, their information about the geographic region of isolation as well as clinical diagnosis were also collected. One hundred and five sequences from N-K2 locus were also obtained as well as the region of isolation, but the information about clinical diagnosis was not available in all cases. The most abundant and widely distributed haplotype in the world is the one of E. histolytica HM1:IMSS strain. This was found in Mexico, Bangladesh, Japan, China and USA and is associated to symptomatic patients as well as asymptomatic cyst passers. Many other haplotypes were found only in a single country. Both genealogies suggest that there are no lineages within the networks that may be related to a particular geographic region or infection outcome. A concatenated analysis of the two molecular markers revealed 12 different combinations, which suggests the possibility of genetic recombination events. The present study is the first to propose a global genealogy of this species and suggests that there are still many genotypes to be discovered. The genotyping of new isolates will help to understand the great diversity and genetic structure of this parasite.
authors.affiliation: Departamento de Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México, México DF, Mexico.
authors.forename: Valeria
authors.initials: V
authors.lastname: Zermeño
authors.forename: Cecilia
authors.initials: C
authors.lastname: Ximénez
authors.forename: Patricia
authors.initials: P
authors.lastname: Morán
authors.forename: Alicia
authors.initials: A
authors.lastname: Valadez
authors.forename: Olivia
authors.initials: O
authors.lastname: Valenzuela
authors.forename: Edgar
authors.initials: E
authors.lastname: Rascón
authors.forename: Daniel
authors.initials: D
authors.lastname: Diaz
authors.forename: René
authors.initials: R
authors.lastname: Cerritos
mesh_terms: D000569:Americas; D001208:Asia; D004748:Entamoeba histolytica / Q000235:genetics*; D004749:Entamoebiasis / Q000453:epidemiology* / Q000469:parasitology; D005789:Genealogy and Heraldry; D056426:Genetic Loci; D014943:Global Health; D006239:Haplotypes; D006801:Humans; D008800:Mexico / Q000453:epidemiology; D018895:Microsatellite Repeats / Q000235:genetics; D063868:Patient Outcome Assessment; D011110:Polymorphism, Genetic