title: Cofactor engineering for advancing chemical biotechnology.
abstract: Cofactors provide redox carriers for biosynthetic reactions, catabolic reactions and act as important agents in transfer of energy for the cell. Recent advances in manipulating cofactors include culture conditions or additive alterations, genetic modification of host pathways for increased availability of desired cofactor, changes in enzyme cofactor specificity, and introduction of novel redox partners to form effective circuits for biochemical processes and biocatalysts. Genetic strategies to employ ferredoxin, NADH and NADPH most effectively in natural or novel pathways have improved yield and efficiency of large-scale processes for fuels and chemicals and have been demonstrated with a variety of microbial organisms.
authors.affiliation: Department of Biochemistry and Cell Biology, Rice University, Houston, TX 77005, USA.
authors.forename: Yipeng
authors.initials: Y
authors.lastname: Wang
authors.forename: Ka-Yiu
authors.initials: KY
authors.lastname: San
authors.forename: George N
authors.initials: GN
authors.lastname: Bennett
mesh_terms: D001709:Biotechnology / Q000379:methods*; D003067:Coenzymes / Q000096:biosynthesis / Q000378:metabolism*; D005288:Ferredoxins / Q000378:metabolism; D060847:Metabolic Engineering / Q000379:methods*; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D013379:Substrate Specificity
chemical_list: D003067:Coenzymes; D005288:Ferredoxins; D009243:NAD; D009249:NADP