title: [Effect of banxia xiexin decoction on leptin and endothelin-1 of gastric ulcer rat and the optimal combination screening of active components].
abstract: OBJECTIVE
  To select the optimal combination of five active component of Banxia Xiexin Decoction on gastric ulcer rat, and observe its effect on Leptin and ET-1.
  
  
  METHODS
  Eighty-seven SD rats were randomly divided into normal group, sham-operated group and acetic acid-induced gastric ulcer group, omeprazole group as a positive control, five active components (glycyrrhetic acid, beta-sitosterol, berberine, baicalin and ginsenoside) of Banxia Xiexin Decoction were divided into groups by L16 orthogonal design. The ulcer area, and the content of Leptin and ET-1, and the mRNA expression level of both were detected.
  
  
  RESULTS
  Among the sixteen orthogonal design groups, the ulcer area of these groups using both beta-sitosterol and berberine was the smallest (P < 0.05), the content of Leptin of these groups using both glycyrrhetic acid and ginsenoside was the highest in blood serum (P < 0.05), the group using glycyrrhetic acid had the minimum concentration of ET-1 in blood plasma. Compared with model group, berberine could raise the mRNA expression level of Leptin (P < 0.01), and beta-sitosterol could lower the mRNA expression level of ET-1 (P < 0.01).
  
  
  CONCLUSIONS
  The pathogenesis of gastric ulcer may be related with the down-regulation of concentration and mRNA expression level of Leptin, and upregulation of concentration and mRNA expression level of ET-1, the active components in Banxia Xiexin Decoction may upregulated Leptin and inhibit ET-1 to accelerate the healing of gastric ulcer.
authors.affiliation: Medical College, Jinan University, Guangzhou 510632, China. youran032003@yahoo.com.cn
authors.forename: Xue-Bing
authors.initials: XB
authors.lastname: Liang
authors.forename: Jun
authors.initials: J
authors.lastname: Sun
authors.forename: Guo-Ping
authors.initials: GP
authors.lastname: Zhao
mesh_terms: D000085:Acetates / Q000009:adverse effects; D000818:Animals; D001599:Berberine / Q000008:administration & dosage; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry* / Q000494:pharmacology*; D019332:Endothelin-1 / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D005419:Flavonoids / Q000008:administration & dosage; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D020738:Leptin / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D008297:Male; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012855:Sitosterols / Q000008:administration & dosage; D013276:Stomach Ulcer / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology
chemical_list: D000085:Acetates; D004365:Drugs, Chinese Herbal; D019332:Endothelin-1; D005419:Flavonoids; D020738:Leptin; D012333:RNA, Messenger; D012855:Sitosterols; C518364:Xiexin decoction; D001599:Berberine; C038044:baicalin; C025473:gamma-sitosterol