title: Report: Simultaneous determination of naphazoline hydrochloride, chlorpheniramine maleate and methylene blue in their ternary mixture.
abstract: Validated spectrophotometric and chemometric methods were developed for determination of Naphazoline Hydrochloride (NAP), Chlorpheniramine maleate (CLO) and Methylene blue (MB) in their ternary mixture. Method A was a spectrophotometric method, where NAP and MB were determined using second derivative (D²) spectrophoto metric method using the peak amplitudes at 299 nm and 337 nm for NAP and MB respectively , while CLO was determined using second derivative ratio (DD²) spectrophotometric method using the peak amplitude at 276.6 nm. Method B used the chemometric techniques; principal component regression (PCR) and partial least squares (PLS) for determination of NAP, CLO and MB using the information contained in the absorption spectra of their ternary mixture solutions. The proposed methods have been successfully applied for the analysis of NAP, CLO and MB in their pharmaceutical formulation and the obtained results were statistically compared with the reported methods.
authors.affiliation: Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Alshaheed Shehata Ahmed Hegazy, Egypt. dr.nourali@hotmail.com
authors.forename: Nouruddin Wageih
authors.initials: NW
authors.lastname: Ali
authors.forename: Maha Ahmad
authors.initials: MA
authors.lastname: Hegazy
authors.forename: Mohamad
authors.initials: M
authors.lastname: Abdelkawy
authors.forename: Rehab Magdy
authors.initials: RM
authors.lastname: Abdelfatah
mesh_terms: D002744:Chlorpheniramine / Q000032:analysis* / Q000737:chemistry; D045424:Complex Mixtures / Q000737:chemistry*; D016018:Least-Squares Analysis; D008751:Methylene Blue / Q000032:analysis* / Q000737:chemistry; D009278:Naphazoline / Q000032:analysis* / Q000737:chemistry; D012996:Solutions / Q000737:chemistry; D013053:Spectrophotometry / Q000379:methods
chemical_list: D045424:Complex Mixtures; D012996:Solutions; D002744:Chlorpheniramine; D009278:Naphazoline; D008751:Methylene Blue