title: Expression of two zona pellucida genes is regulated by 17α-ethinylestradiol in adult rare minnow Gobiocypris rarus.
abstract: Zona pellucida (ZP) proteins are glycoproteins synthesized in liver, ovary or in both tissues in fish. In the present study, we aimed to determine the responsiveness of ZP2 and ZP3 to 17α-ethinylestradiol (EE2) in adult rare minnow Gobiocypris rarus. The full length of ZP3 cDNA was firstly characterized and its tissue distribution revealed that ZP3 mRNA was predominantly expressed in ovary of G. rarus. The gene expression profiles of ZP2, ZP3 and vitellogenin (VTG) were analyzed in gonad and liver of adult G. rarus exposed to EE2 at 1, 5, 25, and 125 ng/L for 3 and 6 days. The results show that ZP2 is more sensitive than ZP3 in gonads of both genders, and VTG in liver is extremely sensitive to EE2 in male fish. However, at lower concentrations (1 and 5 ng/L), the ZP2 in testes shows higher responsiveness to EE2 compared with VTG in rare minnow. The 5' flanking regions of ZP2 and ZP3 were isolated and the comparison of transcription factors in the regions of ZP2 and ZP3 suggested that the disparity for the responsiveness of ZP2 and ZP3 to EE2 could partly be a result of differential cis-elements such as oocyte-specific protein (Osp1) binding sites or/and sex-determining region Y (SRY) binding site.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100 China.
authors.forename: Cong
authors.initials: C
authors.lastname: Yuan
authors.forename: Yingying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Guojun
authors.initials: G
authors.lastname: Hu
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.forename: Yao
authors.initials: Y
authors.lastname: Zheng
authors.forename: Jiancao
authors.initials: J
authors.lastname: Gao
authors.forename: Yanping
authors.initials: Y
authors.lastname: Yang
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.forename: Zaizhao
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003530:Cyprinidae / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D018076:DNA, Complementary / Q000235:genetics; D004527:Egg Proteins / Q000235:genetics* / Q000378:metabolism*; D004997:Ethinyl Estradiol / Q000378:metabolism / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D006066:Gonads / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000378:metabolism*; D016415:Sequence Alignment; D014018:Tissue Distribution; D014157:Transcription Factors; D014158:Transcription, Genetic; D014819:Vitellogenins / Q000378:metabolism; D015044:Zona Pellucida / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology*; D000072216:Zona Pellucida Glycoproteins
chemical_list: D018076:DNA, Complementary; D004527:Egg Proteins; D008562:Membrane Glycoproteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; D014157:Transcription Factors; D014819:Vitellogenins; D000072216:Zona Pellucida Glycoproteins; D004997:Ethinyl Estradiol