title: [Effects of the deletion of RsbV gene on virulence of Listeria monocytogenes].
abstract: OBJECTIVE
  To study the effect of RsbV gene deletion on the virulence of Listeria monocytogenes.
  
  
  METHODS
  The fragment with RsbV deletion was generated by gene overlap extension PCR (SOE-PCR) and the mutant with RsbV deletion was obtained by homologous recombination based on the wild strain LM-XS5. The differences in virulence between the two strains were determined by LD50, bacterial counts in liver and spleen, and qRT-PCR experiments.
  
  
  RESULTS
  LD50 of the RsbV gene-deleted strain was 10(4) higher than that of the wild strain. The numbers of gene-deleted strain in the mouse's liver and spleen were significantly fewer than that of the wild strain (P < 0.05). Results of qRT-PCR show that four virulence factors' expression levels of the RsbV gene-deleted strain were significantly lower than that of the wild strain (P < 0.05). The RsbV gene-deleted strain induced a strong immune response in mice against the wild strain.
  
  
  CONCLUSIONS
  RsbV regulates the expression of four virulence gene (inlA, LLO, PlcA and PrfA) of Listeria monocytogenes; The virulence of the RsbV gene-deleted strain is significantly reduced, but it still has good immunogenicity.
authors.affiliation: Key Laboratory of the High Incidence of Local and National Disease of Ministry of' Education, Shihezi University, Shihezi 832003, China. zhangzaichao@sina.cn
authors.forename: Zaichao
authors.initials: Z
authors.lastname: Zhang
authors.forename: Qingling
authors.initials: Q
authors.lastname: Meng
authors.forename: Jun
authors.initials: J
authors.lastname: Qiao
authors.forename: Chuangfu
authors.initials: C
authors.lastname: Chen
authors.forename: Xuepeng
authors.initials: X
authors.lastname: Cai
authors.forename: Lihong
authors.initials: L
authors.lastname: Yang
authors.forename: Weisheng
authors.initials: W
authors.lastname: Wang
authors.forename: Kuojun
authors.initials: K
authors.lastname: Cai
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D008089:Listeria monocytogenes / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity*; D008088:Listeriosis / Q000382:microbiology*; D051379:Mice; D008969:Molecular Sequence Data; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D037521:Virulence Factors