title: Anti-hyperlipidemic effects and potential mechanisms of action of the caffeoylquinic acid-rich Pandanus tectorius fruit extract in hamsters fed a high fat-diet.
abstract: Hyperlipidemia is considered to be one of the greatest risk factors contributing to the prevalence and severity of cardiovascular diseases. In this work, we investigated the anti-hyperlipidemic effect and potential mechanism of action of the Pandanus tectorius fruit extract in hamsters fed a high fat-diet (HFD). The n-butanol fraction of the P. tectorius fruit ethanol extract (PTF-b) was rich in caffeoylquinic acids (CQAs). Administration of PTF-b for 4 weeks effectively decreased retroperitoneal fat and the serum levels of total cholesterol (TC), triglycerides (TG) and low density lipoprotein-cholesterol (LDL-c) and hepatic TC and TG. The lipid signals (fatty acids, and cholesterol) in the liver as determined by nuclear magnetic resonance (NMR) were correspondingly reduced. Realtime quantitative PCR showed that the mRNA levels of PPARα and PPARα-regulated genes such as ACO, CPT1, LPL and HSL were largely enhanced by PTF-b. The transcription of LDLR, CYP7A1, and PPARγ was also upregulated. Treatment with PTF-b significantly stimulated the activation of AMP-activated protein kinase (AMPK) as well as the activity of serum and hepatic lipoprotein lipase (LPL). Together, these results suggest that administration of the PTF-b enriched in CQAs moderates hyperlipidemia and improves the liver lipid profile. These effects may be caused, at least in part, by increasing the expression of PPARα and its downstream genes and by upregulation of LPL and AMPK activities.
authors.affiliation: Pharmacology and Toxicology Research Center, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, China.
authors.forename: Xiaopo
authors.initials: X
authors.lastname: Zhang
authors.forename: Chongming
authors.initials: C
authors.lastname: Wu
authors.forename: Haifeng
authors.initials: H
authors.lastname: Wu
authors.forename: Linghui
authors.initials: L
authors.lastname: Sheng
authors.forename: Yan
authors.initials: Y
authors.lastname: Su
authors.forename: Xue
authors.initials: X
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Luan
authors.forename: Guibo
authors.initials: G
authors.lastname: Sun
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Sun
authors.forename: Yu
authors.initials: Y
authors.lastname: Tian
authors.forename: Yubin
authors.initials: Y
authors.lastname: Ji
authors.forename: Peng
authors.initials: P
authors.lastname: Guo
authors.forename: Xudong
authors.initials: X
authors.lastname: Xu
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000097:blood / Q000378:metabolism; D008078:Cholesterol, LDL / Q000097:blood / Q000378:metabolism; D006224:Cricetinae; D059305:Diet, High-Fat / Q000009:adverse effects*; D005638:Fruit / Q000737:chemistry*; D000960:Hypolipidemic Agents / Q000627:therapeutic use*; D050356:Lipid Metabolism / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D031673:Pandanaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000627:therapeutic use*; D011801:Quinic Acid / Q000031:analogs & derivatives* / Q000627:therapeutic use; D014280:Triglycerides / Q000097:blood / Q000378:metabolism
chemical_list: D008078:Cholesterol, LDL; D000960:Hypolipidemic Agents; D010936:Plant Extracts; D014280:Triglycerides; C472707:caffeoylquinic acid; D011801:Quinic Acid; D002784:Cholesterol