title: Foamy virus Gag p71-p68 cleavage is required for template switch of the reverse transcriptase.
abstract: In contrast to orthoretroviruses, processing of foamy viral p71 Gag is limited to a single cleavage site. Nevertheless, Gag maturation is essential for infectivity, but deletion of p3 results in a modest drop in infectivity. Here, we show that Gag processing of p71 to p68 and p3 is essential for full-length cDNA synthesis, while inactivation of Gag cleavage results in cDNAs containing only the RU5 region; cDNAs encompassing the U3 region were almost undetectable.
authors.affiliation: Universität Würzburg, Institut für Virologie und Immunbiologie, Würzburg, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Spannaus
authors.forename: Anna
authors.initials: A
authors.lastname: Schneider
authors.forename: Maximilian J
authors.initials: MJ
authors.lastname: Hartl
authors.forename: Birgitta M
authors.initials: BM
authors.lastname: Wöhrl
authors.forename: Jochen
authors.initials: J
authors.lastname: Bodem
mesh_terms: D016282:Aspartic Acid Endopeptidases / Q000378:metabolism*; D015153:Blotting, Western; D018076:DNA, Complementary / Q000096:biosynthesis*; D015683:Gene Products, gag / Q000235:genetics / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D011499:Protein Processing, Post-Translational / Q000235:genetics / Q000502:physiology*; D012194:RNA-Directed DNA Polymerase / Q000378:metabolism*; D016092:Spumavirus / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity
chemical_list: D018076:DNA, Complementary; D015683:Gene Products, gag; D012194:RNA-Directed DNA Polymerase; D016282:Aspartic Acid Endopeptidases; C096363:proteinase, foamy virus