title: Molecular viewing of actin polymerizing actions and beyond: combination analysis of single-molecule speckle microscopy with modeling, FRAP and s-FDAP (sequential fluorescence decay after photoactivation).
abstract: Live-cell single-molecule imaging is a powerful tool to elucidate the in vivo biochemistry of cytoskeletal proteins. However, it is often somewhat difficult to interpret how a bulk population of the observed molecule might behave as a whole. We review our recent studies in which the combination of image analysis with modeling and bulk kinetics measurements such as FRAP (fluorescence recovery after photobleaching) clarified basic problems in the regulation of actin remodeling pathways.
authors.affiliation: Laboratory of Single-Molecule Cell Biology, Tohoku University Graduate School of Life Sciences, Aoba-ku, Sendai, Miyagi, 980-8578, Japan. nwatanabe@m.tohoku.ac.jp
authors.forename: Naoki
authors.initials: N
authors.lastname: Watanabe
authors.forename: Sawako
authors.initials: S
authors.lastname: Yamashiro
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Vavylonis
authors.forename: Tai
authors.initials: T
authors.lastname: Kiuchi
mesh_terms: D000199:Actins / Q000737:chemistry*; D003599:Cytoskeleton / Q000378:metabolism; D036681:Fluorescence Recovery After Photobleaching / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D008853:Microscopy / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods; D011554:Pseudopodia / Q000378:metabolism
chemical_list: D000199:Actins