title: Novel pharmacokinetic studies of the Chinese formula Huang-Lian-Jie-Du-Tang in MCAO rats.
abstract: Our previous studies showed that after oral administration of an Huang-Lian-Jie-Du-Tang (HLJDT) decoction, there is a higher concentration of the pure components, berberine, baicalin and gardenoside in the plasma of Middle cerebral artery occlusion (MCAO) rats than in sham-operated rats, The aim of the present study was to determine whether these components could be reliably measured in MCAO rat tissues. First, the plasma concentration-time profiles of berberine, palmatine, baicalin, baicalein and gardenoside were characterised in MCAO rats after oral administration of the aqueous extract of HLJDT. Subsequently, liver, lung and kidney tissues were obtained from sudden death MCAO rats in the absorption phase (0.25 h), the distribution phase (1.0 h) and the elimination phase (8.0 h) after administration of the HLJDT aqueous extract. An HPLC method was developed and validated for the determination of the distribution characteristics of berberine, palmatine, baicalin, baicalein and gardenoside simultaneously from the above-mentioned rat tissues. The results indicated that berberine, palmatine, baicalin and baicalein distributed rapidly and accumulated at high levels in the lung, while gardenoside distributed widely in the lung and the kidney. To the best of our knowledge, this is the first report to describe the distribution of the active ingredients derived from HLJDT in MCAO rat tissues. The tissue distribution results provide a biopharmaceutical basis for the design of the clinic application of HLJDT in cerebrovascular disease.
authors.affiliation: Separation Engineering of Chinese Traditional Medicine Compound, Nanjing University of Chinese Medicine, Nanjing 210029, China. Huaxuzhu@gmail.com
authors.forename: Huaxu
authors.initials: H
authors.lastname: Zhu
authors.forename: Zhilei
authors.initials: Z
authors.lastname: Qian
authors.forename: Feng
authors.initials: F
authors.lastname: He
authors.forename: Mengzhu
authors.initials: M
authors.lastname: Liu
authors.forename: Linmei
authors.initials: L
authors.lastname: Pan
authors.forename: Qichun
authors.initials: Q
authors.lastname: Zhang
authors.forename: Yuping
authors.initials: Y
authors.lastname: Tang
mesh_terms: D000818:Animals; D001599:Berberine / Q000493:pharmacokinetics / Q000627:therapeutic use; D001600:Berberine Alkaloids / Q000493:pharmacokinetics / Q000627:therapeutic use; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics* / Q000627:therapeutic use; D044950:Flavanones / Q000493:pharmacokinetics / Q000627:therapeutic use; D005419:Flavonoids / Q000493:pharmacokinetics / Q000627:therapeutic use; D020244:Infarction, Middle Cerebral Artery / Q000188:drug therapy*; D039823:Iridoids / Q000493:pharmacokinetics / Q000627:therapeutic use; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution
chemical_list: D001600:Berberine Alkaloids; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005419:Flavonoids; D039823:Iridoids; C045607:huang-lien-chieh-tu-tang; D001599:Berberine; C038044:baicalin; C006680:baicalein; C005413:palmatine; C056587:gardenoside
keywords: A sensitive and reliable HPLC method; Distribution characteristics; Five active ingredients derived from HLJDT; Huang-Lian-Jie-Du-Tang; MCAO rat tissues