title: Determination of retinol and α-tocopherol in human seminal plasma using an HPLC with UV detection.
abstract: Oxidative stress has been proposed as one of the potential causes for infertility in men. Retinol and α-tocopherol have an important role in the spermatozoa defences against oxidative stress. A method is described here for the simultaneous determination of retinol and α-tocopherol in human seminal plasma with a suitable sample preparation procedure to prevent retinol and α-tocopherol degradation. After adequate sample preparation, the samples were determined by reversed-phase column chromatography with UV detection. The analytical performance of this method was satisfactory. The intra-assay and inter-assay coefficients of variation were below 10%. The recoveries were as follows: 90.7% (CV 8.1%) for retinol and 98.2% (CV 4.8%) for α-tocopherol. No significant differences in both retinol and α-tocopherol concentration between the smokers and nonsmokers (15 ± 7 nm and 1.86 ± 0.29 μm versus 15 ± 6 nm and 1.93 ± 0.45 μm) were found. A selective high-performance liquid chromatography method for the determination of retinol and α-tocopherol in human seminal plasma was developed.
authors.affiliation: Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic.
authors.forename: R
authors.initials: R
authors.lastname: Kanďár
authors.forename: P
authors.initials: P
authors.lastname: Drábková
authors.forename: K
authors.initials: K
authors.lastname: Myslíková
authors.forename: R
authors.initials: R
authors.lastname: Hampl
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase; D006801:Humans; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D012661:Semen / Q000378:metabolism*; D012907:Smoking / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D014801:Vitamin A / Q000378:metabolism*; D055815:Young Adult; D024502:alpha-Tocopherol / Q000378:metabolism*
chemical_list: D014801:Vitamin A; D024502:alpha-Tocopherol
keywords: Butylated hydroxytoluene; high-performance liquid chromatography; retinol; seminal plasma; tocopherol