title: Separation, antitumor activities, and encapsulation of polypeptide from Chlorella pyrenoidosa.
abstract: Chlorella pyrenoidosa is a unicellular green algae and has been a popular foodstuff worldwide. However, no reports on the antitumor peptides from such a microalgae are available in the literature. In this study, using low-temperature high-pressure extraction, enzymatic hydrolysis, ion exchange, and gel filtration chromatography, we separated a polypeptide that exhibited inhibitory activity on human liver cancer HepG2 cells, and named the polypeptide CPAP (C. pyrenoidosa antitumor polypeptide). Furthermore, the micro- and nanoencapsulation of CPAP were investigated by using two methods: complex coacervation and ionotropic gelation. The in vitro release tests revealed that CPAP was well preserved against gastric enzymatic degradation after micro/nanoencapsulation and the slowly controlled release in the intestine could be potentially achieved. These results suggest that CPAP may be a useful ingredient in food, nutraceutical, and pharmaceutical applications.
authors.affiliation: Lab. of Biotechnology, College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, P.R. China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Wang
authors.forename: Xuewu
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000493:pharmacokinetics / Q000494:pharmacology*; D002708:Chlorella / Q000737:chemistry*; D002850:Chromatography, Gel; D010450:Endopeptidases; D056945:Hep G2 Cells; D006801:Humans; D053769:Nanocapsules / Q000737:chemistry; D010316:Particle Size; D010446:Peptide Fragments / Q000737:chemistry / Q000302:isolation & purification / Q000493:pharmacokinetics / Q000494:pharmacology; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification / Q000493:pharmacokinetics / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000493:pharmacokinetics / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D053769:Nanocapsules; D010446:Peptide Fragments; D010455:Peptides; D010936:Plant Extracts; D010940:Plant Proteins; D010450:Endopeptidases