title: The growth, lipid and hydrocarbon production of Botryococcus braunii with attached cultivation.
abstract: The green alga Botryococcus braunii is regarded as a potential source of renewable fuel due to its high lipid and hydrocarbon contents. However, the slow growth rate damaged its feasibility for biofuel production. In this study, a novel method of 'attached cultivation' was introduced to incubate B. braunii FACHB 357 (B race). A high biomass productivity of 6.5 gm(-2)d(-1) was achieved in single layer attached system at early stage of cultivation. At day 10, the biomass, lipid and hydrocarbon productivities were 5.5, 2.34 and 1.06 gm(-2)d(-1), respectively. Under nitrogen starvation condition, both of the contents of lipid and hydrocarbon were increased, whereas the profile of hydrocarbon kept almost unchanged, while the content for oleic acid (18:1) increased and linolenic acid (18:3) decreased. With a multi-layer photobioreactor, a biomass productivity of 49.1 gm(-2)d(-1) or a photosynthetic efficiency of 14.9% (visible light) were obtained under continuous illumination of 500 μmolm(-2)s(-1).
authors.affiliation: Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, PR China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Cheng
authors.forename: Bei
authors.initials: B
authors.lastname: Ji
authors.forename: Lili
authors.initials: L
authors.lastname: Gao
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Junfeng
authors.initials: J
authors.lastname: Wang
authors.forename: Tianzhong
authors.initials: T
authors.lastname: Liu
mesh_terms: D018533:Biomass; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D000460:Chlorophyta / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism* / Q000528:radiation effects; D005227:Fatty Acids / Q000378:metabolism; D006838:Hydrocarbons / Q000378:metabolism*; D008027:Light; D008055:Lipids / Q000096:biosynthesis*; D009584:Nitrogen / Q000494:pharmacology; D058433:Photobioreactors
chemical_list: D005227:Fatty Acids; D006838:Hydrocarbons; D008055:Lipids; D009584:Nitrogen