title: Misexpression experiment of Tbx5 in axolotl (Ambystoma mexicanum) hindlimb blastema.
abstract: Axolotls (Ambystoma mexicanum) have the ability to regenerate amputated limbs throughout their life span. In the present study, we attempted to elucidate how axolotls can specify limb type correctly during the regeneration process. We misexpressed Tbx5 in regenerating hindlimb blastema, and consequently a forelimb-like hindlimb regenerated from the hindlimb blastema. On the other hand, no change was observed in Tbx5-overexpressing forelimb blastema, and thus we considered that Tbx5 plays a key role in the specification of forelimb during the regeneration process of axolotl limbs. However, axolotls' fore- and hindlimbs have very similar structures except for the number of fingers, and it was very difficult to judge whether the forelimb-like regenerate was a true forelimb or merely a forelimb-like hindlimb. Therefore, in order to confirm our conclusion, we have to investigate other genes that are expressed differentially between fore- and hindlimbs in future experiments.
authors.affiliation: Division of Anatomy II, Kanazawa Medical University, 1-1 Daigaku, Uchinada-machi, Kahoku-gun, 920-0293 Japan. simoort1@kanazawa-med.ac.jp
authors.forename: Takashi
authors.initials: T
authors.lastname: Shimokawa
authors.forename: Rieko
authors.initials: R
authors.lastname: Kominami
authors.forename: Satoru
authors.initials: S
authors.lastname: Yasutaka
authors.forename: Harumichi
authors.initials: H
authors.lastname: Shinohara
mesh_terms: D000558:Ambystoma mexicanum / Q000378:metabolism*; D000671:Amputation, Surgical; D000818:Animals; D005552:Forelimb / Q000502:physiology / Q000601:surgery; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D006614:Hindlimb / Q000502:physiology* / Q000601:surgery; D012038:Regeneration / Q000502:physiology*; D020825:T-Box Domain Proteins / Q000378:metabolism*
chemical_list: D020825:T-Box Domain Proteins; C103172:T-box transcription factor 5