title: Microanalysis of stomach cancer glycosaminoglycans.
abstract: Glycosaminoglycans (GAGS) are anionic, linear, polysaccharides involved in cell signaling. The GAG content, composition and structure of human tissue have been suggested to play a role in cancer and might provide useful diagnostic or prognostic markers. The current study examines 17 stomach tissue biopsy samples taken from normal individuals and from patients with gastric cancers. An ultrasensitive liquid chromatography (LC) - mass spectrometry assay was applied to individual biopsy samples as small 250 μg providing GAG content and disaccharide composition. The results of these analyses show a significant increase in non-sulfated chondroitin/dermatan sulfate concentration in all cancer samples when compared to normal tissues. In addition in advanced gastric cancer, a significant decrease is observed in hyaluronan.
authors.affiliation: Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, 110 8th Street, Troy, NY, 12180, USA.
authors.forename: Amanda
authors.initials: A
authors.lastname: Weyers
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
authors.forename: Jong-Hwan
authors.initials: JH
authors.lastname: Park
authors.forename: Yong-Seok
authors.initials: YS
authors.lastname: Kim
authors.forename: Sun-Moon
authors.initials: SM
authors.lastname: Kim
authors.forename: Sang-Eok
authors.initials: SE
authors.lastname: Lee
authors.forename: Fuming
authors.initials: F
authors.lastname: Zhang
authors.forename: Kyung Bok
authors.initials: KB
authors.lastname: Lee
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Linhardt
mesh_terms: D000236:Adenoma / Q000378:metabolism*; D000328:Adult; D000368:Aged; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006025:Glycosaminoglycans / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000378:metabolism*
chemical_list: D006025:Glycosaminoglycans