title: The human secretome atlas initiative: implications in health and disease conditions.
abstract: Proteomic analysis of human body fluids is highly challenging, therefore many researchers are redirecting efforts toward secretome profiling. The goal is to define potential biomarkers and therapeutic targets in the secretome that can be traced back in accessible human body fluids. However, currently there is a lack of secretome profiles of normal human primary cells making it difficult to assess the biological meaning of current findings. In this study we sought to establish secretome profiles of human primary cells obtained from healthy donors with the goal of building a human secretome atlas. Such an atlas can be used as a reference for discovery of potential disease associated biomarkers and eventually novel therapeutic targets. As a preliminary study, secretome profiles were established for six different types of human primary cell cultures and checked for overlaps with the three major human body fluids including plasma, cerebrospinal fluid and urine. About 67% of the 1054 identified proteins in the secretome of these primary cells occurred in at least one body fluid. Furthermore, comparison of the secretome profiles of two human glioblastoma cell lines to this new human secretome atlas enabled unambiguous identification of potential brain tumor biomarkers. These biomarkers can be easily monitored in different body fluids using stable isotope labeled standard proteins. The long term goal of this study is to establish a comprehensive online human secretome atlas for future use as a reference for any disease related secretome study. This article is part of a Special Issue entitled: An Updated Secretome.
authors.affiliation: Center for Genetic Medicine Research, Children's National Medical Center, Washington DC 20010, USA.
authors.forename: Kristy J
authors.initials: KJ
authors.lastname: Brown
authors.forename: Haeri
authors.initials: H
authors.lastname: Seol
authors.forename: Dinesh K
authors.initials: DK
authors.lastname: Pillai
authors.forename: Binu-John
authors.initials: BJ
authors.lastname: Sankoorikal
authors.forename: Catherine A
authors.initials: CA
authors.lastname: Formolo
authors.forename: Jenny
authors.initials: J
authors.lastname: Mac
authors.forename: Nathan J
authors.initials: NJ
authors.lastname: Edwards
authors.forename: Mary C
authors.initials: MC
authors.lastname: Rose
authors.forename: Yetrib
authors.initials: Y
authors.lastname: Hathout
mesh_terms: D015415:Biomarkers / Q000032:analysis / Q000097:blood / Q000378:metabolism; D001826:Body Fluids / Q000737:chemistry / Q000378:metabolism; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D005909:Glioblastoma / Q000097:blood / Q000175:diagnosis / Q000378:metabolism; D006801:Humans; D020543:Proteome / Q000032:analysis / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D015415:Biomarkers; D020543:Proteome
keywords: Biomarker; Body fluid; Glioblastoma; Primary cell; Secretome; Stable isotope