title: Physicochemical and biological characterization of pep-1/elastin complexes.
abstract: Transdermal drug delivery of proteins is challenging because the skin acts as a natural and protective barrier. Several techniques including using the cell-penetrating peptides have been studied to increase the penetration of therapeutic proteins into and through the skin. Cell-penetrating peptides facilitate and improve the transduction of large and hydrophilic cargo molecules through plasma membrane. We have recently reported an efficient skin delivery of elastin protein in complex with a cell-penetrating peptide called Pep-1. As the biophysical characteristics of cell-penetrating peptide/protein complexes have been linked with their biological responses, in this study, we investigated biophysical properties of Pep-1/elastin complexes (ratio 10:1) stored in three temperatures (-20 °C, 4 °C and 25 °C) by photon correlation spectroscopy, circular dichroism and isothermal denaturation. We also evaluated the ability of transduction of this complex into cells and skin tissue using both fluorescence microscopy and Kodak In-Vivo FX Pro Imaging System.
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Tehran University of Medical Sciences, Tehran 1417614411, Iran.
authors.forename: Saman
authors.initials: S
authors.lastname: Ahmad Nasrollahi
authors.forename: Changiz
authors.initials: C
authors.lastname: Taghibiglou
authors.forename: Shamileh
authors.initials: S
authors.lastname: Fouladdel
authors.forename: Rasoul
authors.initials: R
authors.lastname: Dinarvand
authors.forename: Ali A
authors.initials: AA
authors.lastname: Moosavi Movahedi
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Azizi
authors.forename: Effat S
authors.initials: ES
authors.lastname: Farboud
mesh_terms: D000279:Administration, Cutaneous; D000595:Amino Acid Sequence; D000818:Animals; D002463:Cell Membrane Permeability; D057846:Cell-Penetrating Peptides / Q000737:chemistry*; D003543:Cysteamine / Q000031:analogs & derivatives* / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry*; D004549:Elastin / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008969:Molecular Sequence Data; D041681:NIH 3T3 Cells; D010455:Peptides / Q000737:chemistry*; D012867:Skin / Q000378:metabolism; D012869:Skin Absorption
chemical_list: D057846:Cell-Penetrating Peptides; D004337:Drug Carriers; C503924:Pep-1 peptide; D010455:Peptides; D003543:Cysteamine; D004549:Elastin
keywords: cell permeability; cell-penetrating peptides; circular dichroism; globular nanoparticles; photon correlation spectroscopy