title: Cloning, tissue expression and polymorphisms of chicken Krüppel-like factor 7 gene.
abstract: Krüppel-like factor 7 (KLF7) has been extensively studied in mammalian species, but its role in birds is still unclear. In the current study, cloning and sequencing showed that the full-length coding region of chicken KLF7 (Gallus gallus KLF7, gKLF7) was 891 bp long, encoding 296 amino acids. In addition, real-time RT-PCR analysis showed that gKLF7 was broadly expressed in all 15 chicken tissues selected, and its expression was significantly different in spleen, proventriculus, abdominal fat, brain, leg muscle, gizzard and heart between fat and lean broilers at 7 weeks of age. Additionally, one novel single nucleotide polymorphism (SNP), XM_426569.3: c. A141G, was identified in the second exon of gKLF7. Association analysis showed that this locus was significantly associated with fatness traits in Arbor Acres broiler random population and the eighth generation of Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHLF) population (P < 0.05). These results suggest that gKLF7 might be a candidate gene for chicken fatness traits.
authors.affiliation: Key Laboratory of Chicken Genetics and Breeding, Ministry of Agriculture, Harbin 150030, China.
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Zhang
authors.forename: Zhi-Peng
authors.initials: ZP
authors.lastname: Wang
authors.forename: Kun
authors.initials: K
authors.lastname: Zhang
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.forename: Hui
authors.initials: H
authors.lastname: Li
mesh_terms: D050153:Abdominal Fat; D000818:Animals; D001823:Body Composition / Q000235:genetics*; D001835:Body Weight / Q000235:genetics*; D002645:Chickens / Q000235:genetics*; D003001:Cloning, Molecular; D051741:Kruppel-Like Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014018:Tissue Distribution
chemical_list: C480503:KLF7 protein, human; D051741:Kruppel-Like Transcription Factors
keywords: Krüppel-like factor 7 (KLF7); SNP; chicken; cloning; expression