title: An electrochemical glutathione biosensor: ubiquinone as a transducer.
abstract: In this paper, coenzyme Q10 (Ubiquinone, CoQ10) was used for the first time as a transducer to construct electrochemical biosensor for effectively detecting γ-L-glutamyl-L-cysteinyl-glycine (glutathione, GSH). CoQ10 modified electrode was fabricated by attaching its gel mixed with multi-walled carbon nanotubes (MWNTs)/ionic liquid (IL). In the optimum conditions, based on the increasing of reduction peak current of CoQ10 caused by GSH through voltammetric technology, it was found that the peak current of CoQ10 was linear with the concentration of GSH in the range from 4.0×10(-9) to 2.0×10(-7)mol L(-1) at the pH 7.00, and the limit of detection was 3.2×10(-10)mol L(-1) (S/N=3). The results revealed that this method could be used to determine GSH in actual blood samples with the superiority of excellent selectivity, high stability and sensitivity. The strategy explored here might provide a new pathway to design novel multi-function transducers for detecting GSH, which has unique characteristic and potential application in the fields of sensor and medical diagnosis.
authors.affiliation: Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou 730070, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ru
authors.forename: Jie
authors.initials: J
authors.lastname: Du
authors.forename: Dong-Dong
authors.initials: DD
authors.lastname: Qin
authors.forename: Bao-Mei
authors.initials: BM
authors.lastname: Huang
authors.forename: Zhong-Hua
authors.initials: ZH
authors.lastname: Xue
authors.forename: Xi-Bin
authors.initials: XB
authors.lastname: Zhou
authors.forename: Xiao-Quan
authors.initials: XQ
authors.lastname: Lu
mesh_terms: D015374:Biosensing Techniques; D055664:Electrochemical Techniques / Q000295:instrumentation*; D005978:Glutathione / Q000032:analysis*; D006801:Humans; D057230:Limit of Detection; D014451:Ubiquinone / Q000737:chemistry*
chemical_list: D014451:Ubiquinone; D005978:Glutathione