title: Serum antibody response to group II chaperonin from Methanobrevibacter oralis and human chaperonin CCT.
abstract: Both group I (HSP60) and group II (CCT) chaperonins are targets of autoantibodies. Autoimmune reactions to HSP60 have been well characterized, while immune reactions to group II chaperonin have not been clarified. Methanobrevibacter oralis is a suspected periodontal pathogen with group II chaperonin. In this study, serum responses to M. oralis chaperonin, human HSP60, and CCT subunits were examined using sera from patients with periodontitis and autoimmune diseases. In comparison with healthy controls, periodontitis patients showed significantly higher responses to CCT4 and CCT8 on dot blot analysis. Signals for CCT3 and CCT8 in autoimmune disease patients were significantly higher than in controls. Significant differences were also demonstrated by Western blotting in anti-CCT4 response in both patient groups. All subjects showed strong reactivity to M. oralis chaperonin and faint signals to human HSP60. Autoantibodies were raised against CCT rather than HSP60; and CCT3, CCT4, and CCT8 were shown to be the main targets. Host immune systems may be frequently exposed to chaperonins of Archaea in various habitats. Although further studies of the cross-reactivity between M. oralis chaperonin and human CCT are required, anti-CCT autoantibodies may be involved in the pathogenesis of periodontitis and autoimmune diseases.
authors.affiliation: Department of Pathophysiology-Periodontal Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors.forename: Kimito
authors.initials: K
authors.lastname: Hirai
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Maeda
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Omori
authors.forename: Tadashi
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Susumu
authors.initials: S
authors.lastname: Kokeguchi
authors.forename: Shogo
authors.initials: S
authors.lastname: Takashiba
mesh_terms: D000328:Adult; D000368:Aged; D000907:Antibodies, Bacterial / Q000097:blood; D001323:Autoantibodies / Q000097:blood; D001327:Autoimmune Diseases / Q000097:blood* / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology; D018834:Chaperonin 60 / Q000276:immunology* / Q000494:pharmacology; D056404:Chaperonin Containing TCP-1 / Q000276:immunology*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D008297:Male; D041581:Methanobrevibacter / Q000276:immunology*; D008875:Middle Aged; D010518:Periodontitis / Q000097:blood* / Q000276:immunology; D021122:Protein Subunits / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology / Q000494:pharmacology
chemical_list: D000907:Antibodies, Bacterial; D001323:Autoantibodies; D001426:Bacterial Proteins; D018834:Chaperonin 60; D007074:Immunoglobulin G; D021122:Protein Subunits; D011994:Recombinant Proteins; D056404:Chaperonin Containing TCP-1