title: [Cultivation of Panax ginseng adventitious roots in bubble bioreactors].
abstract: OBJECTIVE
  To study cultivation of Panax ginseng adventitious roots in bubble bioreactors.
  
  
  METHODS
  The adventitious roots were obtained through tissue culture different types of bioreactors. The contents of ginsenosides Re, Rb1 and Rg1 were determined by HPLC while the contents of polysaccharides were determined by ultraviolet spectrophotometry.
  
  
  RESULTS
  The results showed that of the three types tested, the most efficient bioreactor for cultivation of the ginseng adventitious roots was the cone-type bioreactors (with the 120 degrees ), in which, the growth curve of adventitious roots was S-shaped. The maximum biomass was obtained on the 40th day, with the fresh weight, dry weight and growth rate reaching the maximum, which were 113.15 g, 9.62 g and 63.13 times respectively, and the concomitant contents of polysaccharide and ginsenoside were 2.73% and 2.25 mg x g(-1).
  
  
  CONCLUSIONS
  The results showed that the most efficient bioreactor for cultivation of the ginseng adventitious roots was the cone-type bioreactors (with the 120 degrees). These results provide a theoretical reference for developing an efficient production process of active metabolites of ginseng in the scale-up cultivation.
authors.affiliation: Institute of Biological Engineering of Traditional Chinese Medicine, Tianjin University of Science and Technology, Tianjin 300457, China.
authors.forename: Bei-Mei
authors.initials: BM
authors.lastname: Zuo
authors.forename: Wen-Yuan
authors.initials: WY
authors.lastname: Gao
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.forename: Shuang-Shuang
authors.initials: SS
authors.lastname: Yin
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Zhang
mesh_terms: D018533:Biomass; D019149:Bioreactors / Q000145:classification / Q000592:standards*; D002851:Chromatography, High Pressure Liquid; D036145:Ginsenosides / Q000378:metabolism; D005894:Panax / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics* / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism; D015203:Reproducibility of Results; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors; D046509:Tissue Culture Techniques / Q000379:methods*
chemical_list: D036145:Ginsenosides; D011134:Polysaccharides; C049864:ginsenoside Re; C442759:ginsenoside Rb1; C035054:ginsenoside Rg1