title: Tbx3 and Nr5α2 play important roles in pig pluripotent stem cells.
abstract: Pigs are valuable animal models in pre-clinical research due to their anatomical and similarity to human-beings. Little is known about porcine embryonic development and porcine pluripotent stem cells. Recently, porcine-induced pluripotent stem cells (piPSCs) have been generated with Oct4 (Pou5f1), Sox2, Klf4 and c-Myc (termed OSKM, 4 F). Here, we found two other factors (Tbx3 and Nr5α2, termed TN), with important roles in piPSCs induction. They could improve the generation of piPSCs by supplementing these two factors on the basis of OSKM (OSKMTN, 6 F) orientated to mouse ESCs-like. Surprisingly, Nr5α2 alone could induce piPSCs formation in the presence or absence of c-Myc. These results suggested that Tbx3 and Nr5α2 may have vital roles in Sus scrofa and proposed new insights into pig pluripotent stem cells.
authors.affiliation: College of Life Science, Northeast Agricultural University of China, Harbin, 150030, People's Republic China.
authors.forename: Jianyu
authors.initials: J
authors.lastname: Wang
authors.forename: Qi
authors.initials: Q
authors.lastname: Gu
authors.forename: Jie
authors.initials: J
authors.lastname: Hao
authors.forename: Yundan
authors.initials: Y
authors.lastname: Jia
authors.forename: Binghua
authors.initials: B
authors.lastname: Xue
authors.forename: Honghong
authors.initials: H
authors.lastname: Jin
authors.forename: Jing
authors.initials: J
authors.lastname: Ma
authors.forename: Renyue
authors.initials: R
authors.lastname: Wei
authors.forename: Tang
authors.initials: T
authors.lastname: Hai
authors.forename: Qingran
authors.initials: Q
authors.lastname: Kong
authors.forename: Gerelchimeg
authors.initials: G
authors.lastname: Bou
authors.forename: Ping
authors.initials: P
authors.lastname: Xia
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhou
authors.forename: Liu
authors.initials: L
authors.lastname: Wang
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D058732:Embryoid Bodies / Q000166:cytology / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D005455:Fluorescent Antibody Technique; D015870:Gene Expression; D018398:Homeodomain Proteins / Q000235:genetics; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D000090062:Kruppel-Like Factor 4; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D050814:Octamer Transcription Factor-3 / Q000235:genetics; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055748:SOXB1 Transcription Factors / Q000235:genetics; D033581:Stem Cell Transplantation / Q000379:methods; D013552:Swine; D020825:T-Box Domain Proteins / Q000235:genetics*; D013724:Teratoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D013997:Time Factors; D014183:Transplantation, Heterologous
chemical_list: D018398:Homeodomain Proteins; C000715467:KLF4 protein, human; C000715468:Klf4 protein, mouse; D000090062:Kruppel-Like Factor 4; D050814:Octamer Transcription Factor-3; D018160:Receptors, Cytoplasmic and Nuclear; D055748:SOXB1 Transcription Factors; D020825:T-Box Domain Proteins; D000469:Alkaline Phosphatase