title: Sparstolonin B suppresses lipopolysaccharide-induced inflammation in human umbilical vein endothelial cells.
abstract: Sparstolonin B (SsnB) is an isocoumarin compound isolated from the tubers of both Sparganium stoloniferum and Scirpus yagara. We previously demonstrated that SsnB blocked the Toll-like receptor (TLR) 2- and TLR4-triggered inflammatory signaling in macrophages by inhibiting the recruitment of MyD88 to the TIR domains of TLR2 and TLR4. The present study was designed to examine the effects of SsnB on vascular inflammatory responses in human umbilical vein endothelial cells (HUVECs) challenged by lipopolysaccharide (LPS, a TLR4 ligand). We found that SsnB dose-dependently attenuated the LPS-induced expression of interleukin (IL)-1β and monocyte chemoattractant protein 1 both at the transcription and translation levels in HUVEC. LPS-induced endothelial cell adhesion molecules, intercellular adhesion molecular-1 and vascular cell adhesion molecule-1 expressions were also reduced by treatment with SsnB. In addition, co-incubation with SsnB attenuated THP-1 monocyte adhesion to LPS-activated HUVECs. Furthermore, SsnB efficiently suppressed LPS-induced phosphorylation of extracellular -signal-regulated kinase (Erk1/2) and Akt in HUVECs. These findings show that SsnB can suppress endothelial cell inflammation, suggesting that SsnB might be suitable for development as a therapeutic agent for inflammatory cardiovascular disease.
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210046, China. liangqiaoli2008@163.com
authors.forename: Qiaoli
authors.initials: Q
authors.lastname: Liang
authors.forename: Fang
authors.initials: F
authors.lastname: Yu
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Cui
authors.forename: Jin'ao
authors.initials: J
authors.lastname: Duan
authors.forename: Qinan
authors.initials: Q
authors.lastname: Wu
authors.forename: Prakash
authors.initials: P
authors.lastname: Nagarkatti
authors.forename: Daping
authors.initials: D
authors.lastname: Fan
mesh_terms: D018360:Crystallography, X-Ray; D015536:Down-Regulation / Q000187:drug effects* / Q000502:physiology; D006576:Heterocyclic Compounds, 4 or More Rings / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000473:pathology; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D006576:Heterocyclic Compounds, 4 or More Rings; D008070:Lipopolysaccharides; D010936:Plant Extracts; C561322:sparstolonin B