title: Evaluation of ICSH schistocyte measurement guidelines in France.
abstract: BACKGROUND
  The schistocytes are fragmented red blood cells mainly observed in the setting of hemolytic anemias where they remain an important criterion for the diagnosis. As the identification of these cells is still problematic, the International Council for Standardization in Hematology (ICSH) set up a consensus report in November, 2011. The French Group of Cellular Hematology (GFHC) aimed to collect the opinion of French biologists directly confronted to schistocytes measurements, about these guidelines.
  
  
  METHODS
  Among the 578 professionals, 169 (29%) answered to the 10 questions dealing with the identification and measurements of schistocytes as proposed by the ICSH.
  
  
  RESULTS
  A consensus was reached for the urgent need of such guidelines documents, especially in the current background of the European accreditation EN ISO 15189 rules. A traduction in native (French) language was warmly wished in order to facilitate the diffusion of the information. The pathologic threshold for the diagnosis of thrombotic microangiopathic anemia (TMA) (>1%) remained questionable. For half of the biologists, the new fragmented red blood cell (FRC) parameter recently provided by two manufacturers of automated blood cell counters was still doubtful for routine use.
  
  
  CONCLUSIONS
  This survey assessed the impact of international 'guidelines' on the French biological community. The will to implement validated recommendations was strong, reflecting the awareness of the biologists to standardize the laboratory investigations.
authors.affiliation: Service d'Hématologie Biologique, University Hospital, Nancy, France; Sciences Academy, Nancy, France.
authors.forename: J-F
authors.initials: JF
authors.lastname: Lesesve
authors.forename: H
authors.initials: H
authors.lastname: El Adssi
authors.forename: J
authors.initials: J
authors.lastname: Watine
authors.forename: W
authors.initials: W
authors.lastname: Oosterhuis
authors.forename: F
authors.initials: F
authors.lastname: Régnier
mesh_terms: D000743:Anemia, Hemolytic / Q000175:diagnosis* / Q000473:pathology; D004913:Erythrocytes, Abnormal / Q000473:pathology*; D005602:France; D006282:Health Personnel; D006801:Humans; D017410:Practice Guidelines as Topic / Q000592:standards*; D011785:Quality Assurance, Health Care; D011795:Surveys and Questionnaires
keywords: Schistocyte; evidence based medicine; guidelines; thrombotic microangiopathy