title: Simultaneous capture and in situ analysis of circulating tumor cells using multiple hybrid nanoparticles.
abstract: Using hybrid nanoparticles (HNPs), we demonstrate simultaneous capture, in situ protein expression analysis, and cellular phenotype identification of circulating tumor cells (CTCs). Each HNP consists of three parts: (i) antibodies that bind specifically to a known biomarker for CTCs, (ii) a quantum dot that emits fluorescence signals, and (iii) biotinylated DNA that allows capture and release of CTC-HNP complex to an in-house developed capture & recovery chip (CRC). To evaluate our approach, cells representative of different breast cancer subtypes (MCF-7: luminal; SK-BR-3: HER2; and MDA-MB-231: basal-like) were captured onto CRC and expressions of EpCAM, HER2, and EGFR were detected concurrently. The average capture efficiency of CTCs was 87.5% with identification accuracy of 92.4%. Subsequently, by cleaving the DNA portion with restriction enzymes, captured cells were released at efficiencies of 86.1%. Further studies showed that these recovered cells are viable and can proliferate in vitro. Using HNPs, it is possible to count, analyze in situ protein expression, and culture CTCs, all from the same set of cells, enabling a wide range of molecular- and cellular-based studies using CTCs.
authors.affiliation: In Vitro Diagnostics Lab, Bio Research Center, Samsung Advanced Institute of Technology (SAIT), Samsung Electronics Co., Ltd., Kyunggi-do 446-712, Republic of Korea.
authors.forename: Hun Joo
authors.initials: HJ
authors.lastname: Lee
authors.forename: Hyeon-Yeol
authors.initials: HY
authors.lastname: Cho
authors.forename: Jin Ho
authors.initials: JH
authors.lastname: Oh
authors.forename: Kak
authors.initials: K
authors.lastname: Namkoong
authors.forename: Jeong Gun
authors.initials: JG
authors.lastname: Lee
authors.forename: Jong-Myeon
authors.initials: JM
authors.lastname: Park
authors.forename: Soo Suk
authors.initials: SS
authors.lastname: Lee
authors.forename: Nam
authors.initials: N
authors.lastname: Huh
authors.forename: Jeong-Woo
authors.initials: JW
authors.lastname: Choi
mesh_terms: D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D000951:Antigens, Neoplasm / Q000097:blood / Q000302:isolation & purification; D014408:Biomarkers, Tumor / Q000097:blood*; D001710:Biotin / Q000737:chemistry; D001943:Breast Neoplasms / Q000097:blood* / Q000175:diagnosis; D015815:Cell Adhesion Molecules / Q000097:blood / Q000302:isolation & purification; D004247:DNA / Q000737:chemistry; D000071858:Epithelial Cell Adhesion Molecule; D066246:ErbB Receptors / Q000097:blood / Q000302:isolation & purification; D005260:Female; D005453:Fluorescence; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D061986:MCF-7 Cells; D053758:Nanoparticles / Q000737:chemistry*; D009360:Neoplastic Cells, Circulating / Q000276:immunology*; D045663:Quantum Dots / Q000737:chemistry; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000097:blood / Q000302:isolation & purification
chemical_list: D000906:Antibodies; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D001710:Biotin; D015815:Cell Adhesion Molecules; D004247:DNA; D000071858:Epithelial Cell Adhesion Molecule; D066246:ErbB Receptors; D018719:Erb-b2 Receptor Tyrosine Kinases; C499379:EPCAM protein, human; C512478:EGFR protein, human; C508053:ERBB2 protein, human