title: Biodegradation of nicotine by a novel nicotine-degrading bacterium, Pseudomonas plecoglossicida TND35 and its new biotransformation intermediates.
abstract: Tobacco wastes that contain nicotine alkaloids are harmful to human health and the environment. In the investigation, a novel nicotine-biodegrading bacterium TND35 was isolated and identified as Pseudomonas plecoglossicida on the basis of phenotypic, biochemical characteristics and 16S rRNA sequence homology. We have studied the nicotine biodegradation potential of strain TND35 by detecting the intermediate metabolites using an array of approaches such as HPLC, GC-MS, NMR and FT-IR. Biotransformation metabolites, N-methylmyosmine, 4-hydroxy-1-(3-pyridyl)-1-butanone (HPB) and other three new intermediate metabolites namely, 3,5-bis (1-methylpyrrolidin-2-yl) pyridine, 2,3-dihydro-1-methyl-5-(pyridin-3-yl)-1H-pyrrol-2-ol and 5-(pyridin-3-yl)-1H-pyrrol-2(3H)-one have been identified. Interestingly, these intermediate metabolites suggest that the strain TND35 employs a novel nicotine biodegradation pathway, which is different from the reported pathways of Aspergillus oryzae 112822, Arthrobacter nicotinovorans pAO1, Agrobacterium tumefaciens S33 and other species of Pseudomonas. The metabolite, HPB reported in this study can also be used as biochemical marker for tobacco related cancer studies.
authors.affiliation: Department of Biotechnology, School of Life Sciences, Pondicherry University, Puducherry, India.
authors.forename: Gurusamy
authors.initials: G
authors.lastname: Raman
authors.forename: KasiNadar
authors.initials: K
authors.lastname: Mohan
authors.forename: Venkat
authors.initials: V
authors.lastname: Manohar
authors.forename: Natarajan
authors.initials: N
authors.lastname: Sakthivel
mesh_terms: D001673:Biodegradation, Environmental; D001711:Biotransformation; D002074:Butanones / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry; D007700:Kinetics; D009538:Nicotine / Q000378:metabolism*; D011549:Pseudomonas / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011725:Pyridines / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000145:classification / Q000235:genetics*; D012989:Soil Pollutants / Q000378:metabolism*; D062789:Tobacco Products; D014866:Waste Products
chemical_list: D002074:Butanones; D011725:Pyridines; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D014866:Waste Products; D009538:Nicotine; C482281:4-hydroxy-1-(3-pyridyl)-1-butanone