title: Phenotyping jasmonate regulation of senescence.
abstract: Osmotic stress induces several senescence-like processes in leaves, such as specific changes in gene expression and yellowing. These processes are dependent on the accumulation of jasmonates and on intact jasmonate signaling. This chapter describes the treatment of Arabidopsis thaliana leaves with sorbitol as an osmotic stress agent and the determination of the elicited phenotypes encompassing chlorophyll loss, degradation of plastidial membrane lipids, and induction of genes regulated by senescence and jasmonate.
authors.affiliation: Pharmaceutical Biology, Julius-von-Sachs-Institute for Biosciences, University of Wuerzburg, Wuerzburg, Germany.
authors.forename: Martin A
authors.initials: MA
authors.lastname: Seltmann
authors.forename: Susanne
authors.initials: S
authors.lastname: Berger
mesh_terms: D017360:Arabidopsis / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D002734:Chlorophyll / Q000302:isolation & purification / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism*; D004075:Diglycerides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D015870:Gene Expression; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D008563:Membrane Lipids / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D009997:Osmotic Pressure; D054883:Oxylipins / Q000378:metabolism*; D010641:Phenotype; D010937:Plant Growth Regulators / Q000502:physiology*; D018515:Plant Leaves / Q000502:physiology*; D018087:Plastids / Q000378:metabolism; D015398:Signal Transduction; D013012:Sorbitol / Q000494:pharmacology
chemical_list: D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D004075:Diglycerides; D008563:Membrane Lipids; D054883:Oxylipins; D010937:Plant Growth Regulators; D002734:Chlorophyll; D013012:Sorbitol; C011006:jasmonic acid