title: Wound healing effect of flavonoid rich fraction and luteolin isolated from Martynia annua Linn. on streptozotocin induced diabetic rats.
abstract: OBJECTIVE
  To evaluate wound healing potential of flavonoid fractions of Martynia annua (M. annua) Linn. leaves in diabetic rats on the basis of folkloric information and preliminary study.
  
  
  METHODS
  The flavonoid compound luteolin and apigenin were isolated from dried leaves of plant by column chromatography. The two concentrations (0.2% and 0.5% w/w) of luteolin and flavonoid fraction were selected for topically applied as ointment on diabetic wound. The Povidone Iodine Ointment USP was used as a reference. On 18th days, protein content, hydroxyproline and antioxidants (SOD, CAT and GSH) level in granuloma tissues were determined.
  
  
  RESULTS
  The results showed that, percent wound contraction were observed significantly (P<0.01) greater in MAF fraction and 0.5% w/w of luteolin treatment groups. Presence of matured collagen fibres and fibroblasts with better angiogenesis were observed in histopathological studies.
  
  
  CONCLUSIONS
  In conclusion, our findings suggest that flavonoid fraction (MAF) and luteolin (0.5% w/w) may have potential benefit in enhancing wound healing in diabetic condition, possibly due to free-radical scavenging activity of plant.
authors.affiliation: Department of Pharmaceutical Sciences, Dr. H. S. Gour University, Sagar (M.P.), India.
authors.forename: Santram
authors.initials: S
authors.lastname: Lodhi
authors.forename: Abhay K
authors.initials: AK
authors.lastname: Singhai
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000473:pathology / Q000503:physiopathology; D004305:Dose-Response Relationship, Drug; D003875:Drug Eruptions / Q000209:etiology; D005260:Female; D005347:Fibroblasts / Q000187:drug effects; D005419:Flavonoids / Q000008:administration & dosage / Q000009:adverse effects / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000494:pharmacology; D006909:Hydroxyproline / Q000378:metabolism; D047311:Luteolin / Q000008:administration & dosage / Q000009:adverse effects / Q000494:pharmacology; D008297:Male; D009824:Ointments; D029601:Pedaliaceae; D008517:Phytotherapy / Q000379:methods*; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D018515:Plant Leaves; D011506:Proteins / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000737:chemistry; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D016166:Free Radical Scavengers; D009824:Ointments; D010936:Plant Extracts; D011506:Proteins; D047311:Luteolin; D006909:Hydroxyproline