title: Separation and characterization of dextran extracted from deteriorated sugarcane.
abstract: In this research work, dextran was extracted from deteriorated sugarcane by alcohol precipitation and purified by gel filtration chromatography. Total acid hydrolysis and enzymatic degradation were utilized to confirm the purity of separated polysaccharide. Using the more recently available techniques such as ((1)H,(13)C) and two-dimensional (COSY and HMQC) NMR spectral analysis, methylation GC-MS and MALDI-TOF mass spectrometry, the structure of sugarcane dextran (SC-Dex) was investigated. On the basis of all spectra, SC-Dex showed a branched polysaccharide that contained only d-glucose residues in consecutive α-(1-6) linkages in the main chain with α-(1-3) branches. Methylation analysis showed that, the degree of α-(1-3) branching levels was 4.37%. Several structural fragments were identified from MALDI-TOF spectrum with peak-to-peak mass difference of 162gmol(-1), which confirmed that the repeat unit in SC-Dex was d-glucose. The surface morphology of SC-Dex, revealed the spherically shaped and porous structure. Using HPSEC-MALLS-RI system, the average molecular weight of SC-Dex was estimated to be 1.753×10(6)gmol(-1) with an index of polydispersity value of 1.069.
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China. mohanad.81@hotmail.com
authors.forename: Mohanad
authors.initials: M
authors.lastname: Bashari
authors.forename: Camel
authors.initials: C
authors.lastname: Lagnika
authors.forename: Denis
authors.initials: D
authors.lastname: Ocen
authors.forename: Haiying
authors.initials: H
authors.lastname: Chen
authors.forename: Jinpeng
authors.initials: J
authors.lastname: Wang
authors.forename: Xueming
authors.initials: X
authors.lastname: Xu
authors.forename: Zhengyu
authors.initials: Z
authors.lastname: Jin
mesh_terms: D002850:Chromatography, Gel; D003911:Dextrans / Q000737:chemistry* / Q000302:isolation & purification; D005947:Glucose / Q000737:chemistry; D006868:Hydrolysis; D008745:Methylation; D031786:Saccharum / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014866:Waste Products
chemical_list: D003911:Dextrans; D014866:Waste Products; D005947:Glucose