title: Protective effects of Keemun black tea polysaccharides on acute carbon tetrachloride-caused oxidative hepatotoxicity in mice.
abstract: This study was designed to investigate chemical characterization of the water-soluble polysaccharides extracted from Keemun black tea (KBTP), and their antioxidant and hepatoprotective effects against CCl4-induced oxidative damage in mice. HPLC analysis revealed that KBTP is the typical acidic heteropolysaccharides and consisted of nine monosaccharides. Furthermore, KBTP showed highly ferric-reducing antioxidant power and scavenging effects against DPPH, OH and O2(-) in vitro. Administration of KBTP (200, 400 and 800 mg/kg bw) in mice ahead of CCl4 injection could observably antagonize the CCl4-induced increases in serum ALT, AST, TG and TC, and the hepatic MDA and 8-iso-PGF2a levels, respectively. Mice with KBTP pretreatment displayed a better profile of hepatosomatic index and the improved GSH and SOD activities in comparison with CCl4-intoxicated mice. These biochemical results were further supported with liver histopathological assessment, revealing that KBTP has an observable prevention of liver damage induced by CCl4 in mice.
authors.affiliation: Key Laboratory of Ministry of Education for Medicinal Resource and Natural Pharmaceutical Chemistry, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an 710062, China.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Sun
authors.forename: Xingbin
authors.initials: X
authors.lastname: Yang
authors.forename: Xinshan
authors.initials: X
authors.lastname: Lu
authors.forename: Dongying
authors.initials: D
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001835:Body Weight; D002251:Carbon Tetrachloride / Q000633:toxicity*; D056486:Chemical and Drug Induced Liver Injury / Q000209:etiology / Q000517:prevention & control*; D002784:Cholesterol / Q000097:blood; D002851:Chromatography, High Pressure Liquid; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008297:Male; D051379:Mice; D009929:Organ Size; D018384:Oxidative Stress / Q000187:drug effects*; D011134:Polysaccharides / Q000494:pharmacology*; D013662:Tea / Q000737:chemistry*; D014280:Triglycerides / Q000097:blood
chemical_list: D011134:Polysaccharides; D013662:Tea; D014280:Triglycerides; D002784:Cholesterol; D002251:Carbon Tetrachloride; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase
keywords: Antioxidant effect; HPLC; Hepatoprotective activity; Keemun black tea; Liver damage; Polysaccharides