title: Apolipoprotein E and its mimetic peptide suppress Th1 and Th17 responses in experimental autoimmune encephalomyelitis.
abstract: Apolipoprotein E (apoE) has been detected to possess anti-inflammatory properties that can contribute to protection against experimental autoimmune encephalomyelitis (EAE). However, its impact on Th1 and Th17 responses in EAE is unclear. In this study, we induced EAE in apoE-/- mice and wild-type mice. We observed that the absence of apoE resulted in the increased proportion of Th1 and Th17 cells in the spleens and brains, as well as up-regulated expressions of proinflammatory cytokines (IL-17, IFN-γ, TNF-α, IL-12, IL-1β and IL-6) and transcription factors (RORγt and T-bet) in the CNS. ApoE-/- mice also showed the increased release of proinflammatory cytokines by macrophages in vitro. In addition, we used a mimetic peptide of apoE, which mimic the functions of apoE except for lipid transport. ApoE mimetic peptide could reverse the above negative effect in EAE. Thus, apoE can modulate Th1 and Th17 responses, likely through its inhibitory effect on the secretion of cytokines by macrophages. Our result also suggests that apoE mimetic peptide might be developed into a therapeutic agent for multiple sclerosis.
authors.affiliation: Department of Neurology, The First Affiliated Hospital, Guangxi Medical University, Nanning, Guangxi, China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Wei
authors.forename: Minghua
authors.initials: M
authors.lastname: Zheng
authors.forename: Peixiao
authors.initials: P
authors.lastname: Liang
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Wei
authors.forename: Xiaoduan
authors.initials: X
authors.lastname: Yin
authors.forename: Yulan
authors.initials: Y
authors.lastname: Tang
authors.forename: Yimin
authors.initials: Y
authors.lastname: Xue
mesh_terms: D000818:Animals; D001057:Apolipoproteins E / Q000235:genetics / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000188:drug therapy* / Q000473:pathology; D005260:Female; D005434:Flow Cytometry; D007150:Immunohistochemistry; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D063308:Myelin-Oligodendrocyte Glycoprotein / Q000494:pharmacology; D010446:Peptide Fragments / Q000494:pharmacology; D010455:Peptides / Q000494:pharmacology; D060888:Real-Time Polymerase Chain Reaction; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D018417:Th1 Cells / Q000187:drug effects*; D058504:Th17 Cells / Q000187:drug effects*
chemical_list: D001057:Apolipoproteins E; D016207:Cytokines; D063308:Myelin-Oligodendrocyte Glycoprotein; D010446:Peptide Fragments; D010455:Peptides