title: Simultaneous isolation of xanthomegnin, viomellein, rubrosulphin, viopurpurin, and brevianamide A by preparative HPLC.
abstract: A method for the isolation of xanthomegnin, viomellein, rubrosulphin, viopurpurin, and brevianamide A from Penicillium viridicatum (DSM 2447) is described. After extraction, HPLC was performed with a preparative silicagel column, eluted with toluene / ethyl acetate / formic acid (27/9/1, v/v/v) and dichloromethane / acetic acid (9/1, v/v). The toxins were detected with a UV-monitor. It was possible to isolate them in an absolutely pure state. The described method is operationally simple and very efficient.
authors.affiliation: Institut für Biochemie, Johannes-Gutenberg-Universität, Johann-Joachim-Becher-Weg 30, D-6500, Mainz, FRG.
authors.forename: C M
authors.initials: CM
authors.lastname: Jansen
authors.forename: K
authors.initials: K
authors.lastname: Dose