title: [2-DG enhances TRAIL-induced apoptosis of leukemia HL-60 cells].
abstract: This study was purposed to investigate the effects of 2-deoxy-D-glucose (2-DG) on sensitizing HL-60 cells to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis and its possible mechanism. The proliferative inhibition of HL-60 cells treated with different concentrations of 2-DG and TRAIL was measured by MTT assay. The cells were treated with 2-DG, TRAIL, and 2-DG combined with TRAIL at the concentration < IC50 value, i.e. 10 mmol/L for 2-DG and 100 ng/ml for TRAIL. Apoptosis was analyzed by flow cytometry with PI staining; the expression of RIP1, GRP78, and PARP was analyzed by Western blot; the activity of caspase-3 was detected by special detection kit. The results showed that the combined treatment of HL-60 cells for 48 h induced an apoptotic rate of (45.1 ± 4.3)%, which was significantly higher than that of treated with 2-DG or TRAIL alone; at the same time, the combined treatment potentiated the expression of GRP78 and caspase-3 activity, and down-regulated the expression of RIP1. It is concluded that 2-DG can sensitize HL-60 cells to TRAIL-induced apoptosis, which may be correlated with excessive endoplasmic reticulum stress response, down-regulation of RIP1, and increase of caspase-3 activity.
authors.affiliation: Anhui Engineering Technology Research Center of Biochemical Pharmaceuticals, Anhui Province, China.
authors.forename: Su-Rong
authors.initials: SR
authors.lastname: Zhao
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Duan
authors.forename: Pei
authors.initials: P
authors.lastname: Zhang
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.forename: Chen-Chen
authors.initials: CC
authors.lastname: Jiang
authors.forename: Zhi-Wen
authors.initials: ZW
authors.lastname: Jiang
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D003847:Deoxyglucose / Q000494:pharmacology*; D000091342:Endoplasmic Reticulum Chaperone BiP; D018922:HL-60 Cells; D006360:Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D028861:Nuclear Pore Complex Proteins / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000378:metabolism; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000378:metabolism / Q000494:pharmacology*
chemical_list: C119724:AGFG1 protein, human; D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; D006360:Heat-Shock Proteins; D028861:Nuclear Pore Complex Proteins; D016601:RNA-Binding Proteins; D053221:TNF-Related Apoptosis-Inducing Ligand; D003847:Deoxyglucose; C505407:CASP3 protein, human; D053148:Caspase 3