title: Molecular cloning, characterization and expression analysis of F-type lectin from pearl oyster Pinctada fucata.
abstract: F-type lectin is an important type of pattern recognition receptor that can recognize and bind carbohydrate moieties on the surface of potential pathogens through its carbohydrate recognition domains (CRDs). This paper reports the cloning of an F-type lectin (designated as pfF-type lectin) from the pearl oyster (Pinctada fucata) using rapid amplification of cDNA ends (RACE) PCR. The full-length cDNA of this pfF-type lectin contains an open reading frame (ORF) of 588 bp coding for196 amino acids. A signal peptide at the N-terminus of the deduced polypeptide was predicted by the signal P program and the cleavage site is located between the positions of Gly(19)and Tyr(20). Conserved domain search at NCBI revealed the pfF-type lectin domain extends from Lys(55)to Val(192). Semi-quantitative analysis in adult tissues showed that the pfF-type lectin mRNA was abundantly expressed in haemocytes and gill and rarely expressed in other tissues tested. After challenge with lipopolysaccharide (LPS), expression of pfF-type lectin mRNA in haemocytes was increased, reaching the highest level at 4 h, then dropping to basal levels at 36 h. These results suggest that F-type lectin play a critical role in the innate immune system of the pearl oyster P. fucata.
authors.affiliation: Marine Biotechnology Division, Central Marine Fisheries Research Institute (CMFRI), Ernakulam North PO, Kochi, India. anju.moonjely@gmail.com
authors.forename: A
authors.initials: A
authors.lastname: Anju
authors.forename: J
authors.initials: J
authors.lastname: Jeswin
authors.forename: P C
authors.initials: PC
authors.lastname: Thomas
authors.forename: K K
authors.initials: KK
authors.lastname: Vijayan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000502:physiology; D037102:Lectins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008070:Lipopolysaccharides / Q000502:physiology; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010802:Phylogeny; D049911:Pinctada / Q000737:chemistry / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment / Q000662:veterinary
chemical_list: D018076:DNA, Complementary; D037102:Lectins; D008070:Lipopolysaccharides; D012333:RNA, Messenger