title: Regulation of milk protein solubility by a whey-derived proline-rich peptide product.
abstract: The effects of a bovine whey peptide product enriched in proline (wPRP) on the solubility of milk proteins were tested under ambient conditions or following heat treatment at 75 and 100 °C, for 1 and 15 min, followed by post-incubation storage at either ambient temperature or 4 °C for up to 7 d. wPRP promoted solubilisation of milk proteins in a concentration-dependent manner without heat treatment and also after heat treatment at 75 and 100 °C, and the effect was enhanced after storage under either ambient or refrigerated storage conditions. Interactions of wPRP and milk proteins were monitored by particle size analysis and tryptic digestion and specifically linked with solubilisation of αS1 casein (αS1-Cn), which supported observed changes in milk protein solubility. The results suggested that wPRP preferably prevented or reversed physical versus covalent protein aggregation, with the relaxation of hydrophobic interactions at 4 °C providing an additive effect. This application of wPRP represents a novel approach to stabilisation of dairy proteins following thermal processing with industrial usefulness yet to be explored.
authors.affiliation: CSIRO Animal, Food and Health Sciences, 671 Sneydes Road, Werribee, Victoria, 3030, Australia.
authors.forename: Sophie N
authors.initials: SN
authors.lastname: Selby-Pham
authors.forename: Kate
authors.initials: K
authors.lastname: Howell
authors.forename: Hema
authors.initials: H
authors.lastname: Jegasothy
authors.forename: Paul
authors.initials: P
authors.lastname: Sheean
authors.forename: Tanoj
authors.initials: T
authors.lastname: Singh
authors.forename: Cheryl
authors.initials: C
authors.lastname: Taylor
authors.forename: Louise E
authors.initials: LE
authors.lastname: Bennett
mesh_terms: D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D056148:Chromatography, Reverse-Phase / Q000379:methods; D019075:Electrophoresis, Capillary / Q000379:methods; D008894:Milk Proteins / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism; D010316:Particle Size; D010455:Peptides / Q000737:chemistry; D011392:Proline; D012995:Solubility; D000067816:Whey Proteins
chemical_list: D008894:Milk Proteins; D010455:Peptides; D000067816:Whey Proteins; D011392:Proline