title: Overexpressions of microRNA-9 and microRNA-200c in human breast cancers are associated with lymph node metastasis.
abstract: OBJECTIVE
  The aim of this study was to investigate the relationship between the expressions of microRNA-9 (miR-9) and microRNA-200c (miR-200c) in human breast cancers and clinicopathological features.
  
  
  METHODS
  We investigated the expressions of miR-9 and miR-200c in 68 patients with breast cancers using the quantitative reverse transcription-polymerase chain reaction method, and assessed the E-cadherin status using the immunohistochemistry method.
  
  
  RESULTS
  The relative expression levels of miR-9 and miR-200c in breast cancer patients with lymph node metastasis were higher than that of patients without lymph node metastasis. The expression of miR-9 correlated inversely with E-cadherin expression.
  
  
  CONCLUSIONS
  The results showed that higher expressions of miR-9 and miR-200c in human breast cancers were associated with lymph node metastasis. This study indicated that the elevation of miR-9 and miR-200c in human breast cancers can induce an invasive phenotype and may serve as a molecular diagnostic marker for patients with breast cancer.
authors.affiliation: 1 Oncobiology Key Lab of Heilongjiang Common Institution of Higher Learning, Department of Medical Oncology, The Affiliated Tumor Hospital of Harbin Medical University , Harbin, Heilongjiang Province, People's Republic of China .
authors.forename: Jincai
authors.initials: J
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Zhao
authors.forename: Dabei
authors.initials: D
authors.lastname: Tang
authors.forename: Jin
authors.initials: J
authors.lastname: Wu
authors.forename: Guodong
authors.initials: G
authors.lastname: Yao
authors.forename: Qingyuan
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D001943:Breast Neoplasms / Q000737:chemistry / Q000235:genetics* / Q000473:pathology*; D015820:Cadherins / Q000032:analysis; D018270:Carcinoma, Ductal, Breast / Q000737:chemistry / Q000235:genetics* / Q000556:secondary*; D005260:Female; D015870:Gene Expression; D006801:Humans; D008207:Lymphatic Metastasis; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism*
chemical_list: D015820:Cadherins; C533896:MIRN200 microRNA, human; C541874:MIRN92 microRNA, human; D035683:MicroRNAs; D012334:RNA, Neoplasm
keywords: E-cadherin; breast cancer; metastasis; microRNA-200c; microRNA-9