title: Genotype and growing environment interaction shows a positive correlation between substrates of raffinose family oligosaccharides (RFO) biosynthesis and their accumulation in chickpea ( Cicer arietinum L.) seeds.
abstract: To develop genetic improvement strategies to modulate raffinose family oligosaccharides (RFO) concentration in chickpea ( Cicer arietinum L.) seeds, RFO and their precursor concentrations were analyzed in 171 chickpea genotypes from diverse geographical origins. The genotypes were grown in replicated trials over two years in the field (Patancheru, India) and in the greenhouse (Saskatoon, Canada). Analysis of variance revealed a significant impact of genotype, environment, and their interaction on RFO concentration in chickpea seeds. Total RFO concentration ranged from 1.58 to 5.31 mmol/100 g and from 2.11 to 5.83 mmol/100 g in desi and kabuli genotypes, respectively. Sucrose (0.60-3.59 g/100 g) and stachyose (0.18-2.38 g/100 g) were distinguished as the major soluble sugar and RFO, respectively. Correlation analysis revealed a significant positive correlation between substrate and product concentration in RFO biosynthesis. In chickpea seeds, raffinose, stachyose, and verbascose showed a moderate broad sense heritability (0.25-0.56), suggesting the use of a multilocation trials based approach in chickpea seed quality improvement programs.
authors.affiliation: Department of Plant Sciences, University of Saskatchewan, 51 Campus Drive Saskatoon, Saskatchewan S7N 5A8, Canada.
authors.forename: Manu P
authors.initials: MP
authors.lastname: Gangola
authors.forename: Yogendra P
authors.initials: YP
authors.lastname: Khedikar
authors.forename: Pooran M
authors.initials: PM
authors.lastname: Gaur
authors.forename: Monica
authors.initials: M
authors.lastname: Båga
authors.forename: Ravindra N
authors.initials: RN
authors.lastname: Chibbar
mesh_terms: D000349:Africa; D001208:Asia; D029863:Cicer / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D004777:Environment; D005838:Genotype; D009844:Oligosaccharides / Q000378:metabolism; D011887:Raffinose / Q000032:analysis / Q000096:biosynthesis*; D012639:Seeds / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D013020:South America; D013395:Sucrose / Q000378:metabolism
chemical_list: D009844:Oligosaccharides; C005695:stachyose; D013395:Sucrose; C570815:verbascose; D011887:Raffinose