title: Efficacy evaluation of the protein isolated from Peganum harmala seeds as an antioxidant in liver of rats.
abstract: OBJECTIVE
  To evaluate the curative effect of the 132 KD protein isolated from the seeds of Peganum harmala (P. harmala) L. against carbon tetrachloride (CCl(4)) induced oxidative stress in rats.
  
  
  METHODS
  Animals were post treated intraperitoneally with 132 KD isolated protein at doses of 4 and 8 mg/kg body weight and bovine serum albumin (BSA) (8 mg/kg body weight) as well as vitamin C (250 mg/kg body weight p.o.) for 7 d after they challenged with CCl(4) orally (1 mL/kg body weight) in olive oil (50%) for 2 d.
  
  
  RESULTS
  The purified protein from seeds of P. harmala plant showed in vitro antioxidant activity with DPPH assay. Administration of CCl(4) induced induction in serum aminotransferases (AST, ALT), alkaline phosphatase (ALP), lipid profile parameters and liver malondialdehyde (MDA) and decrease in serum total protein, liver superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH) levels. 132 KD protein treatment of rats post CCl4 intoxication successfully alleviated the toxic effects of CCl(4).
  
  
  CONCLUSIONS
  The isolated protein possessed strong antioxidant activity comparable to that of BSA (negative control) and vitamin C (positive control).
authors.affiliation: Zoology Department, Faculty of Science, Cairo University, Egypt. soliman.amel5@gmail.com
authors.forename: Amel M
authors.initials: AM
authors.lastname: Soliman
authors.forename: Helal S
authors.initials: HS
authors.lastname: Abu-El-Zahab
authors.forename: Gamiaa A
authors.initials: GA
authors.lastname: Alswiai
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D002251:Carbon Tetrachloride / Q000633:toxicity; D002374:Catalase / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism / Q000517:prevention & control; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000378:metabolism; D007274:Injections, Intraperitoneal; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D032443:Peganum; D008517:Phytotherapy / Q000379:methods*; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D010940:Plant Proteins / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D012639:Seeds; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D010940:Plant Proteins; D002251:Carbon Tetrachloride; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione