title: Anticancer activities of (-)-epigallocatechin-3-gallate encapsulated nanoliposomes in MCF7 breast cancer cells.
abstract: The chemopreventive actions exerted by green tea are thought to be due to its major polyphenol, (-)-epigallocatechin-3-gallate (EGCG). However, the low level of stability and bioavailability in the body makes administering EGCG at chemopreventive doses unrealistic. We synthesized EGCG encapsulated chitosan-coated nanoliposomes (CSLIPO-EGCG), and observed their antiproliferative and proapoptotic effect in MCF7 breast cancer cells. CSLIPO-EGCG significantly enhanced EGCG stability, improved sustained release, increased intracellular EGCG content in MCF7 cells, induced apoptosis of MCF7 cells, and inhibited MCF7 cell proliferation compared to native EGCG and void CSLIPO. The CSLIPO-EGCG retained its antiproliferative and proapoptotic effectiveness at 10 μM or lower, at which native EGCG does not have any beneficial effects. This study portends a potential breakthrough in the prevention or even treatment of breast cancer by using biocompatible and biodegradable CSLIPO-EGCG with enhanced chemopreventive efficacy and minimized immunogenicity and side-effects.
authors.affiliation: Department of Nutrition, Texas Tech University, Lubbock, TX, USA.
authors.forename: Rita Castillo Cohen
authors.initials: RC
authors.lastname: de Pace
authors.forename: Xinli
authors.initials: X
authors.lastname: Liu
authors.forename: Ming
authors.initials: M
authors.lastname: Sun
authors.forename: Shufang
authors.initials: S
authors.lastname: Nie
authors.forename: Jia
authors.initials: J
authors.lastname: Zhang
authors.forename: Qingsong
authors.initials: Q
authors.lastname: Cai
authors.forename: Weimin
authors.initials: W
authors.lastname: Gao
authors.forename: Xuan
authors.initials: X
authors.lastname: Pan
authors.forename: Zhaoyang
authors.initials: Z
authors.lastname: Fan
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
mesh_terms: D016588:Anticarcinogenic Agents / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms / Q000188:drug therapy; D002392:Catechin / Q000008:administration & dosage / Q000031:analogs & derivatives*; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry; D005260:Female; D006801:Humans; D008081:Liposomes; D061986:MCF-7 Cells; D058726:Nanoconjugates / Q000008:administration & dosage*
chemical_list: D016588:Anticarcinogenic Agents; D008081:Liposomes; D058726:Nanoconjugates; D002392:Catechin; D048271:Chitosan; C045651:epigallocatechin gallate