title: Seasonal abundance and potential of Japanese encephalitis virus infection in mosquitoes at the nesting colony of ardeid birds, Thailand.
abstract: OBJECTIVE
  To investigate the abundance and seasonal dynamics of mosquitoes, and to detect Japanese encephalitis virus (JEV) in these mosquitoes at the nesting colony of ardeid birds.
  
  
  METHODS
  Mosquitoes were collected bimonthly from July 2009 to May 2010 by Centers for Disease Control. Light traps and dry ice, as a source of CO2, were employed to attract mosquitoes. Mosquitoes were first identified, pooled into groups of upto 50 mosquitoes by species, and tested for JEV infection by viral isolation and reverse transcriptase polymerase chain reaction.
  
  
  RESULTS
  A total of 20 370 mosquitoes comprising 14 species in five genera were collected. The five most abundant mosquito species collected were Culex tritaeniorhynchus (95.46%), Culex vishnui (2.68%), Culex gelidus (0.72%), Anopheles peditaeniatus (0.58%) and Culex quinquefasciatus (0.22%). Mosquito peak densities were observed in July. All of 416 mosquito pools were negative for JEV.
  
  
  CONCLUSIONS
  This study provides new information about mosquito species and status of JEV infection in mosquitoes in Thailand. Further study should be done to continue a close survey for the presence of this virus in the ardeid birds.
authors.affiliation: The Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals, Faculty of Veterinary Science, Mahidol University, Nakorn Pathom, Thailand.
authors.forename: Tanasak
authors.initials: T
authors.lastname: Changbunjong
authors.forename: Thekhawet
authors.initials: T
authors.lastname: Weluwanarak
authors.forename: Namaoy
authors.initials: N
authors.lastname: Taowan
authors.forename: Parut
authors.initials: P
authors.lastname: Suksai
authors.forename: Tatiyanuch
authors.initials: T
authors.lastname: Chamsai
authors.forename: Poonyapat
authors.initials: P
authors.lastname: Sedwisai
mesh_terms: D000818:Animals; D001715:Bird Diseases / Q000453:epidemiology* / Q000821:virology; D001717:Birds; D009033:Culicidae / Q000502:physiology / Q000821:virology*; D004664:Encephalitis Virus, Japanese / Q000302:isolation & purification*; D004672:Encephalitis, Japanese / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D011157:Population Dynamics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012621:Seasons; D013785:Thailand / Q000453:epidemiology; D014776:Virus Cultivation / Q000662:veterinary
keywords: Abundance; Ardeid bird; Japanese encephalitis virus; Mosquito; Vector