title: Construction of a functional silk-based biomaterial complex with immortalized chondrocytes in vivo.
abstract: To explore the feasibility of constructing a functional biomaterial complex with regenerated silk fibroin membrane and immortalized chondrocytes in vivo. Rat auricular chondrocytes (RACs) were transfected with the lentivirus vector pGC-FU-hTERT-3FLAG or pGC-FU-GFP-3FLAG, encoding the human telomerase reverse transcriptase (hTERT) or GFP gene. The effects of regenerated silk fibroin film on the adhesion, growth of immortalized chondrocytes and expression of collagen II in vitro were analyzed with immunofluorescent histochemistry. Immortalized RACs were transformed. Induction by nutrient medium promoted higher expression levels of collagen II in transformed chondrocytes. The regenerated silk fibroin film was not cytotoxic to immortalized chondrocytes and had no adverse influence on their adhesion. Collagen II expression was good in the immortalized chondrocytes in vivo. The construction of a silk-based biomaterial complex with immortalized chondrocytes may provide a feasible kind of functional biomaterial for the repair of cartilage defects in clinical applications.
authors.affiliation: Department of Otolaryngology, Eye and ENT Hospital of Shanghai Medical School, Fudan University, Shanghai, China.
authors.forename: Yusu
authors.initials: Y
authors.lastname: Ni
authors.forename: Yi
authors.initials: Y
authors.lastname: Jiang
authors.forename: Jianchuan
authors.initials: J
authors.lastname: Wen
authors.forename: Zhenzhong
authors.initials: Z
authors.lastname: Shao
authors.forename: Xin
authors.initials: X
authors.lastname: Chen
authors.forename: Shan
authors.initials: S
authors.lastname: Sun
authors.forename: Huiqian
authors.initials: H
authors.lastname: Yu
authors.forename: Wen
authors.initials: W
authors.lastname: Li
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D002461:Cell Line, Transformed; D049109:Cell Proliferation / Q000187:drug effects; D019902:Chondrocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D024043:Collagen Type II / Q000378:metabolism; D016616:Cryoultramicrotomy; D005349:Fibroins / Q000494:pharmacology / Q000648:ultrastructure; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D008297:Male; D018613:Microscopy, Confocal; D051381:Rats; D017207:Rats, Sprague-Dawley; D047011:Silk / Q000494:pharmacology*; D040521:Subcutaneous Tissue / Q000187:drug effects / Q000473:pathology; D019098:Telomerase / Q000378:metabolism; D054457:Tissue Scaffolds / Q000737:chemistry; D014162:Transfection
chemical_list: D001672:Biocompatible Materials; D024043:Collagen Type II; D047011:Silk; D049452:Green Fluorescent Proteins; D005349:Fibroins; D019098:Telomerase
keywords: biomaterials; cartilage; cytocompatibility; silk fibroin