title: Surfaceomics and surface-enhanced Raman spectroscopy of environmental microbes: matching cofactors with redox-active surface proteins.
abstract: Trypsin shaving is a targeted proteomic method for identifying cell-surface exposed proteins on bacterial cells. For the identification of redox-active cell-surface proteins, trypsin-shaving datasets can be matched with surface-enhanced Raman spectra of intact cells to identify the cofactors associated with the cell-surface proteins. Together, these approaches could help resolve questions about the presence of cell-surface electron transport components in environmental microorganisms, especially microbes that oxidize and reduce metals and metalloids as electron donors and acceptors.
authors.affiliation: Department of Plant and Microbial Biology, University of California, Berkeley, CA, USA.
authors.forename: Hans K
authors.initials: HK
authors.lastname: Carlson
authors.forename: Anthony T
authors.initials: AT
authors.lastname: Iavarone
authors.forename: John D
authors.initials: JD
authors.lastname: Coates
mesh_terms: D001419:Bacteria / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D003067:Coenzymes / Q000378:metabolism*; D004783:Environmental Microbiology; D008565:Membrane Proteins / Q000378:metabolism*; D010084:Oxidation-Reduction; D013059:Spectrum Analysis, Raman; D014357:Trypsin / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D003067:Coenzymes; D008565:Membrane Proteins; D014357:Trypsin
keywords: Microbiology; SERRS; SERS; Surfaceome; Trypsin shaving