title: High-throughput sequencing: a roadmap toward community ecology.
abstract: High-throughput sequencing is becoming increasingly important in microbial ecology, yet it is surprisingly under-used to generate or test biogeographic hypotheses. In this contribution, we highlight how adding these methods to the ecologist toolbox will allow the detection of new patterns, and will help our understanding of the structure and dynamics of diversity. Starting with a review of ecological questions that can be addressed, we move on to the technical and analytical issues that will benefit from an increased collaboration between different disciplines.
authors.affiliation: Département de biologie, chimie et géographie, Université du Québec à Rimouski 300 Allée des Ursulines, Rimouski, QC, G5L 3A1, Canada ; Québec Centre for Biodiversity Sciences, Stewart Biological Sciences Building 1205 Dr. Penfield Avenue, Montréal, QC, H3A 1B1, Canada.
authors.forename: Timothée
authors.initials: T
authors.lastname: Poisot
authors.forename: Bérangère
authors.initials: B
authors.lastname: Péquin
authors.forename: Dominique
authors.initials: D
authors.lastname: Gravel
keywords: Biogeography; community ecology; high-throughput sequencing; microbial ecology