title: Assessment of heavy metal content and DNA damage in Hypsiboas faber (anuran amphibian) in coal open-casting mine.
abstract: The aims of the study were to determine the heavy metal content in the tissues of Hypsiboas faber from a coal mining area and to compare the DNA damage in the blood cells of these animals with that of animals living in an unpolluted area. The heavy metal content was detected according to the technique of Particle-Induced X-ray Emission (PIXE) and the DNA damage was assessed by the Comet assay. Our results reveal that the specimens of H. faber collected from the coal mining area exhibited elements of order Fe>Cu>Al>Zn>Rb>Mn>Br, independently of the organ. The values of Comet assay parameters (DNA damage index and DNA damage frequency) were significantly higher in specimens collected from the coal mining area than in the reference animals. Our study concludes that the coal mining residues are genotoxic to amphibians and may have adverse effects on soil, water, vegetation and wild animals.
authors.affiliation: Laboratório de Ecologia de Paisagem e de Vertebrados, Programa de Pós-Graduação em Ciências Ambientais, Unidade Acadêmica de Humanidades, Ciências e Educação, Universidade do Extremo Sul Catarinense (UNESC), Criciúma, SC, Brazil. jjz@unesc.net
authors.forename: Jairo José
authors.initials: JJ
authors.lastname: Zocche
authors.forename: Adriani Paganini
authors.initials: AP
authors.lastname: Damiani
authors.forename: Giana
authors.initials: G
authors.lastname: Hainzenreder
authors.forename: Rodrigo Ávila
authors.initials: RÁ
authors.lastname: Mendonça
authors.forename: Poliana Bernardo
authors.initials: PB
authors.lastname: Peres
authors.forename: Carla Eliete Iochims Dos
authors.initials: CE
authors.lastname: Santos
authors.forename: Rafaela
authors.initials: R
authors.lastname: Debastiani
authors.forename: Johnny Ferraz
authors.initials: JF
authors.lastname: Dias
authors.forename: Vanessa Moraes de
authors.initials: VM
authors.lastname: Andrade
mesh_terms: D000818:Animals; D001001:Anura / Q000378:metabolism*; D001938:Brazil; D001966:Bromine / Q000032:analysis / Q000633:toxicity; D003032:Coal Mining; D020552:Comet Assay; D004249:DNA Damage; D004784:Environmental Monitoring; D004785:Environmental Pollutants / Q000032:analysis* / Q000633:toxicity; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008297:Male; D008670:Metals / Q000032:analysis* / Q000633:toxicity; D018482:Muscle, Skeletal / Q000737:chemistry
chemical_list: D004785:Environmental Pollutants; D008670:Metals; D001966:Bromine