title: Soil remediation with a microbial community established on a carrier: strong hints for microbial communication during 1,2,4-Trichlorobenzene degradation.
abstract: The objective of the present study was to get more insight into the mechanisms that govern the high mineralization potential of a microbial community attached on a carrier material, as we found in an earlier study (Wang et al., 2010). A 1,2,4-Trichlorobenzene (1,2,4-TCB) degrading microbial community - attached (MCCP) and non-attached (MCLM) on clay particles - was inoculated into a simplified mineral medium system. Signaling molecules (AHLs), cell growth and 1,2,4-TCB mineralization were measured at different sampling points. The production of AHLs in the MCCP system increased continuously with increasing key degrader (Bordetella sp.) cell growth and a positive correlation was observed between the production of AHLs and 1,2,4-TCB mineralization. In the MCLM system, however, 1,2,4-TCB mineralization was lower than in the MCCP system; the AHLs production per Bordetella cell was higher than in MCCP and there was no correlation between AHLs and mineralization. Moreover, in the MCCP system less different AHLs were produced than in the MCLM system. These results indicate that a microbial community attached on a carrier material has an advantage over a non-attached community: it produces signaling molecules with much less energy and effort to achieve a well-directed cell-to-cell communication resulting in a high and effective mineralization.
authors.affiliation: State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Agnes
authors.initials: A
authors.lastname: Fekete
authors.forename: Mourad
authors.initials: M
authors.lastname: Harir
authors.forename: Xiao
authors.initials: X
authors.lastname: Chen
authors.forename: Ulrike
authors.initials: U
authors.lastname: Dörfler
authors.forename: Michael
authors.initials: M
authors.lastname: Rothballer
authors.forename: Xin
authors.initials: X
authors.lastname: Jiang
authors.forename: Philippe
authors.initials: P
authors.lastname: Schmitt-Kopplin
authors.forename: Reiner
authors.initials: R
authors.lastname: Schroll
mesh_terms: D054742:Acyl-Butyrolactones / Q000378:metabolism; D001419:Bacteria / Q000166:cytology / Q000378:metabolism; D001673:Biodegradation, Environmental; D002722:Chlorobenzenes / Q000302:isolation & purification / Q000378:metabolism*; D008903:Minerals / Q000378:metabolism; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D054742:Acyl-Butyrolactones; D002722:Chlorobenzenes; D008903:Minerals; D012987:Soil; D012989:Soil Pollutants; C009947:1,2,4-trichlorobenzene
keywords: 1,2,4-Trichlorobenzene; Carrier material; Cell-to-cell communication; Mineralization; N-acyl-homoserine lactone