title: FAK-Src signalling is important to renal collecting duct morphogenesis: discovery using a hierarchical screening technique.
abstract: This report describes a hierarchical screening technique for identification of pathways that control the morphogenesis of the renal collecting duct system. The multi-step screen involves a first round using a 2-dimensional, cell-line-based scrape-healing assay, then a second round using a 3-dimensional tubulogenesis assay; both of these rounds use new cell lines described in this report. The final stage is ex vivo organ culture. We demonstrate the utility of the screen by using it to identify the FAK-Src-pathway signalling as being important for collecting duct development, specifically for the cell proliferation on which this development depends.
authors.affiliation: Centre for Integrative Physiology, University of Edinburgh , Edinburgh EH8 9XD , UK.
authors.forename: Guangping
authors.initials: G
authors.lastname: Tai
authors.forename: Peter
authors.initials: P
authors.lastname: Hohenstein
authors.forename: Jamie A
authors.initials: JA
authors.lastname: Davies
keywords: Branching morphogenesis; Cell proliferation; Collecting duct; FAK; Kidney; Screening; Src; Tubulogenesis; Ureteric bud