title: Effects of thoracic irradiation on pulmonary endothelial compared to alveolar type-II cells in fibrosis-prone C57BL/6NTac mice.
abstract: OBJECTIVE
  Thoracic irradiation results in an acute inflammatory response, latent period, and late fibrosis. Little is known about the mechanisms involved in triggering late radiation fibrosis.
  
  
  METHODS
  Thoracic irradiated fibrosis prone C57BL/6NTac mice were followed for detectable mRNA transcripts in isolated lung cells and micro-RNA in whole-tissues, and the effect of administration of water-soluble oxetanyl sulfoxide MMS350 was studied. Marrow stromal cell motility in medium from fibrotic-phase explanted pulmonary endothelial and alveolar type-II cells was measured.
  
  
  RESULTS
  RNA and micro-RNA expression in lung correlated with fibrosis. MMS350 reduced pro-fibrotic gene expression in both endothelial and alveolar type-II cells in irradiated mice. Conditioned medium from irradiated cells did not alter cell motility in vitro.
  
  
  CONCLUSIONS
  These studies should facilitate identification of potential new drug targets for ameliorating irradiation-induced pulmonary fibrosis.
authors.affiliation: Professor and Chairman, Department of Radiation Oncology, University of Pittsburgh Cancer Institute, UPMC Cancer Pavilion, 5150 Centre Avenue, Pittsburgh, PA 15232, U.S.A.
authors.forename: Ronny
authors.initials: R
authors.lastname: Kalash
authors.forename: Hebist
authors.initials: H
authors.lastname: Berhane
authors.forename: Julie
authors.initials: J
authors.lastname: Goff
authors.forename: Frank
authors.initials: F
authors.lastname: Houghton
authors.forename: Michael W
authors.initials: MW
authors.lastname: Epperly
authors.forename: Tracy
authors.initials: T
authors.lastname: Dixon
authors.forename: Xichen
authors.initials: X
authors.lastname: Zhang
authors.forename: Melissa M
authors.initials: MM
authors.lastname: Sprachman
authors.forename: Peter
authors.initials: P
authors.lastname: Wipf
authors.forename: Darcy
authors.initials: D
authors.lastname: Franicola
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Joel S
authors.initials: JS
authors.lastname: Greenberger
mesh_terms: D056809:Alveolar Epithelial Cells / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D000818:Animals; D016207:Cytokines / Q000096:biosynthesis; D004195:Disease Models, Animal; D004988:Ethers, Cyclic / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000528:radiation effects; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics; D011650:Pulmonary Alveoli / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D011658:Pulmonary Fibrosis / Q000209:etiology* / Q000235:genetics / Q000473:pathology; D017564:Radiation Pneumonitis / Q000235:genetics / Q000473:pathology; D011878:Radiotherapy / Q000009:adverse effects*; D015398:Signal Transduction / Q000187:drug effects / Q000528:radiation effects; D017154:Stromal Cells / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D013454:Sulfoxides / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects / Q000528:radiation effects
chemical_list: D016207:Cytokines; D004988:Ethers, Cyclic; C586473:MMS350; D035683:MicroRNAs; D013454:Sulfoxides