title: [The influence of representative herbs of clearing and detoxifying drugs effect on inflammatory cytokines expression of mice lung homogenate infected by influenza virus FM1].
abstract: OBJECTIVE
  In order to screen out a certain kind of traditional medicine which has a better role in immune regulatory, the influence of representatives of heat clearing and detoxicating herb on inflammatory cytokines protein expression of mice lung homogenate infected by FM1 have been observed.
  
  
  METHODS
  Modeling mice infected by FM1. On the first, third, fifth and seventh day after FM1 infection, tumor necrosis factor-alpha (TNF-alpha), interleukin-1 (IL-1), interleukin-6 (IL-6), interleukin-10 (IL-10), and gamma-interferon (IFN-gamma) expression in mice lung homogenate of normal control group, model control group, scutellari group, isatidis group, pulsatilla group, polygonum cuspidatum group and oldenlandia group have been tested by ELISA method.
  
  
  RESULTS
  The expression of TNF-alpha, IL-6, IFN-gamma and IL-10 in mice lung homogenate reaches its peak on the third day after FM1 infection, significantly higher than the control group (P < 0.05). Scutellari and isatidis are two representatives of heat clearing and detoxicating herb, which can decrease the expression of TNF-alpha, IL-6 and IL-1 and increase the expression of IL-10, IFN-gamma. The effect are more pronounced and statistically significant (P < 0.05) on the third and fifth day after infection, pulsatilla, polygonum cuspidatum and oldenlandia can also regulate the inflammatory cytokines, but the effect are not so obvious as scutellari and isatidis.
  
  
  CONCLUSIONS
  Scutellari and isatidis, two representatives of heat clearing and detoxicating herb, have a good intervention on immune damage caused by influenza virus through adjusting the balance of inflammatory cytokines and anti-inflammatory cytokines.
authors.affiliation: Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China. wang601@vip.sina.com
authors.forename: Cheng-Xiang
authors.initials: CX
authors.lastname: Wang
authors.forename: Hong-Yun
authors.initials: HY
authors.lastname: Cao
authors.forename: Miao
authors.initials: M
authors.lastname: Cheng
authors.forename: Hong-Ri
authors.initials: HR
authors.lastname: Xu
authors.forename: Ming-Zhe
authors.initials: MZ
authors.lastname: Wang
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Xu
authors.forename: Hui-Yong
authors.initials: HY
authors.lastname: Yu
authors.forename: Qian
authors.initials: Q
authors.lastname: Li
mesh_terms: D000818:Animals; D002642:Chick Embryo; D016207:Cytokines / Q000235:genetics* / Q000276:immunology; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D018836:Inflammation Mediators / Q000276:immunology*; D009980:Influenza A virus / Q000187:drug effects / Q000502:physiology*; D007251:Influenza, Human / Q000188:drug therapy* / Q000235:genetics* / Q000276:immunology / Q000821:virology; D008168:Lung / Q000276:immunology* / Q000821:virology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D016207:Cytokines; D004365:Drugs, Chinese Herbal; D018836:Inflammation Mediators