title: Analysis of glycoproteins for biomarker discovery.
abstract: Glycoproteins play an important role in cell signaling and cell-cell interaction. The alterations of glycoproteins are often relevant to progression of diseases, and these changed glycoproteins can be important biomarkers. The lectin-based glycoproteomic technology has extensively been used for high-throughput screening of potential glycoprotein biomarkers. Here we describe a multi-lectin affinity chromatography and label-free quantitative glycoproteomic approach for discovery of glycoprotein biomarkers relevant to differentiation of glioblastoma stem cells.
authors.affiliation: Department of Surgery, University of Michigan Medical Center, Ann Arbor, MI, USA.
authors.forename: Jintang
authors.initials: J
authors.lastname: He
authors.forename: Yashu
authors.initials: Y
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
authors.forename: David M
authors.initials: DM
authors.lastname: Lubman
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D002450:Cell Communication; D002846:Chromatography, Affinity; D005909:Glioblastoma / Q000378:metabolism*; D006023:Glycoproteins / Q000032:analysis* / Q000737:chemistry; D057166:High-Throughput Screening Assays; D006801:Humans; D014411:Neoplastic Stem Cells / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction
chemical_list: D014408:Biomarkers, Tumor; D006023:Glycoproteins