title: Stat3β mitigates development of atherosclerosis in apolipoprotein E-deficient mice.
abstract: The transcription factor Stat3 is an activator of systemic inflammatory genes. Two isoforms of Stat3 are generated by alternative splicing, Stat3α and Stat3β. The β isoform lacks the transactivation domain but retains other functions, including dimerization and DNA binding. Stat3β-deficient mice exhibit elevated expression of systemic inflammatory genes and are hyperresponsive to lipopolysaccharide, suggesting that Stat3β functions predominantly as a suppressor of systemic inflammation. To test whether Stat3β deficiency would provoke pathologic effects associated with chronic inflammation, we asked whether selective removal of Stat3β would exacerbate the development of atherosclerosis in apolipoprotein E-deficient mice. In apoE(-/-)Stat3β(-/-) mice atherosclerotic plaque formation was significantly enhanced relative to apoE(-/-)Stat3β(+/+) controls. The ability of Stat3β deficiency to promote atherosclerosis was more pronounced in female mice, but could be unmasked in males by feeding a high fat diet. Infiltrating macrophages were not increased in aortas of apoE(-/-)Stat3β(-/-) mice. In contrast, the proportion of pro-inflammatory TH17 cells was significantly elevated in aortic infiltrates from apoE(-/-)Stat3β(-/-) mice, relative to paired apoE(-/-)Stat3β(+/+) littermates. These observations indicate that Stat3β can suppress pathologic sequelae associated with chronic inflammation. Our findings further suggest that in Stat3β-deficient mice the unopposed action of Stat3α may enhance atherogenesis in part by promoting differentiation of TH17 cells.
authors.affiliation: Department of Molecular Biology and Genetics and Institute for Cell Engineering, The Johns Hopkins University School of Medicine, Room 619, 733 N. Broadway, Baltimore, MD 21205, USA.
authors.forename: Jihyun
authors.initials: J
authors.lastname: Lee
authors.forename: William M
authors.initials: WM
authors.lastname: Baldwin
authors.forename: Chih-Yuan
authors.initials: CY
authors.lastname: Lee
authors.forename: Stephen
authors.initials: S
authors.lastname: Desiderio
mesh_terms: D000818:Animals; D001011:Aorta / Q000473:pathology; D001057:Apolipoproteins E / Q000172:deficiency* / Q000235:genetics; D050197:Atherosclerosis / Q000378:metabolism*; D001835:Body Weight; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism; D002478:Cells, Cultured; D002784:Cholesterol / Q000097:blood; D016207:Cytokines / Q000378:metabolism; D059305:Diet, High-Fat; D005260:Female; D017737:Macrophages, Peritoneal / Q000378:metabolism; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D050796:STAT3 Transcription Factor / Q000235:genetics / Q000378:metabolism*; D014280:Triglycerides / Q000097:blood
chemical_list: D001057:Apolipoproteins E; D016207:Cytokines; D050796:STAT3 Transcription Factor; D014280:Triglycerides; D002784:Cholesterol