title: Polyglycerol coatings of glass vials for protein resistance.
abstract: Proteins are surface active molecules which undergo non-specific adsorption when getting in contact with surfaces such as the primary packaging material. This process is critical as it may cause a loss of protein content or protein aggregation. To prevent unspecific adsorption, protein repellent coatings are of high interest. We describe the coating of industrial relevant borosilicate glass vials with linear methoxylated polyglycerol, hyperbranched polyglycerol, and hyperbranched methoxylated polyglycerol. All coatings provide excellent protein repellent effects. The hyperbranched, non-methoxylated coating performed best. The protein repellent properties were maintained also after applying industrial relevant sterilization methods (≥200 °C). Marginal differences in antibody stability between formulations stored in bare glass vials and coated vials were detected after 3 months storage; the protein repellent effect remained largely stable. Here, we describe a new material suitable for the coating of primary packaging material of proteins which significantly reduces the protein adsorption and thus could present an interesting new possibility for biomedical applications.
authors.affiliation: Department for Pharmacy, Pharmaceutical Technology and Biopharmaceutics, Ludwig-Maximilians University, Munich, Germany.
authors.forename: Kerstin
authors.initials: K
authors.lastname: Höger
authors.forename: Tobias
authors.initials: T
authors.lastname: Becherer
authors.forename: Wei
authors.initials: W
authors.lastname: Qiang
authors.forename: Rainer
authors.initials: R
authors.lastname: Haag
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Friess
authors.forename: Sarah
authors.initials: S
authors.lastname: Küchler
mesh_terms: D000327:Adsorption; D001896:Boron Compounds / Q000737:chemistry; D004349:Drug Packaging; D004356:Drug Storage; D005898:Glass / Q000737:chemistry*; D005990:Glycerol / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D055550:Protein Stability; D011506:Proteins / Q000737:chemistry*; D017640:Silicates / Q000737:chemistry; D013242:Sterilization; D013997:Time Factors
chemical_list: D001896:Boron Compounds; D007074:Immunoglobulin G; D011108:Polymers; D011506:Proteins; D017640:Silicates; C043941:polyglycerol; D005990:Glycerol
keywords: Glass vials; HPG(OH); HPG(OMe); Hyperbranched polyglycerol; LPG(OMe); PEG; PG; Packaging material; Protein adsorption; Surface coating; hyperbranched methoxylated polyglycerol; hyperbranched polyglycerol; linear methoxylated polyglycerol; poly(ethylene glycol); polyglycerol