title: Starchy biomass-powered enzymatic biofuel cell based on amylases and glucose oxidase multi-immobilized bioanode.
abstract: The present study reports the design of a novel bioanode to directly utilize starch as a fuel in an enzymatic biofuel cell. The enzymatic fuel cell is based on three enzymes (alpha-amylase, glucoamylase and glucose oxidase). The carbon paste electrode containing these three enzymes and tetrathiafulvalene can both saccharize and oxidize starchy biomass. In cyclic voltammetry, catalytic currents were successfully observed with both glucose and starchy white rice used as a substrate. Finally, a membrane-less white rice/O2 biofuel cell was assembled and the electrochemical performance was evaluated. The three enzyme based electrode was used as a bioanode and an immobilized bilirubin oxidase (derived from Myrothecium verrucaria) electrode was used as a biocathode. The biofuel cell delivered an open circuit voltage of 0.522V and power density of up to 99.0 μWcm(-2). Our results show that a readily available fuel can be used for enzymatic fuel cells, and will lead to new designs.
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada, Kobe 657-8501, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Takuya
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Shota
authors.initials: S
authors.lastname: Shimada
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Tanaka
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
mesh_terms: D000681:Amylases / Q000737:chemistry*; D001203:Ascomycota / Q000201:enzymology; D001426:Bacterial Proteins / Q000737:chemistry; D001674:Bioelectric Energy Sources; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry; D005087:Glucan 1,4-alpha-Glucosidase / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry*; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000737:chemistry*; D013213:Starch / Q000737:chemistry*; D016985:Streptococcus bovis / Q000201:enzymology
chemical_list: D001426:Bacterial Proteins; D004800:Enzymes, Immobilized; D005656:Fungal Proteins; D013213:Starch; D005949:Glucose Oxidase; D044925:Oxidoreductases Acting on CH-CH Group Donors; C042401:bilirubin oxidase; D000681:Amylases; D005087:Glucan 1,4-alpha-Glucosidase