title: [Expression of PD-L1 in lymphoma and its effect on lymphoma proliferation and chemotherapy resistance].
abstract: In order to investigate the effect of PD-L1 on lymphoma cell proliferation and chemotherapy resistance, and explore a new and promising approach for therapy of lymphomas. The level of PD-L1 expression in tissues of patients with lymphoma and cell lines were detected by immunohistochemistry and flow cytometry. Both the stable high-PDL1-A20 cell strain and low-PDL1-A20 cell strain were sorted by flow cytometry, then inoculated into BALB/c mice as to establish a lymphoma bearing mouse model for observing tumor genesis. Finally, the effect of PD-L1 on routinely chemotherapy drug-cisplatin were observed. The results showed that the PD-L1 highly and widely expressed in lymphoma tissue and cell strains, the stable high-PD-L1 cell strain sorted by flow cytometry have higher lymphoma genesis than that of low-PD-L1 cell strain. It is concluded that PD-L1 can promote lymphoma genesis, meanwhile the PD-L1 may resist effect of cisplatin on lymphoma cells through anti-apoptosis.
authors.affiliation: Department of Hematology and Lymphoma Research Center, Peking University Third Hospital, Beijing, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Ke
mesh_terms: D000818:Animals; D017209:Apoptosis; D060890:B7-H1 Antigen / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002945:Cisplatin / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm; D008223:Lymphoma / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL
chemical_list: D060890:B7-H1 Antigen; D002945:Cisplatin