title: Human leucocyte antigen-defined microchimerism early post-transplant does not predict for stable lung allograft function.
abstract: Microchimerism is the presence of foreign cells in an individual below 1% of total cells, which can occur in the setting of solid organ transplantation. This study quantitated donor-derived cellular subsets longitudinally in human leucocyte antigen (HLA)-mismatched lung transplant recipients (LTR) during the first post-operative year and evaluated the pattern of peripheral microchimerism with clinical outcomes. Peripheral blood mononuclear cells (PBMC) isolated from non-HLA-B44 LTR who received HLA-B44 allografts were sorted flow cytometrically into three cellular subsets. Real-time quantitative polymerase chain reaction (q-PCR) demonstrated that donor-derived HLA-B44 microchimerism is a common phenomenon, observed in 61% of patients. The level of donor-derived cells varied across time and between LTR with frequencies of 38% in the B cells/monocytes subset, 56% in the T/NK cells subset and 11% in the dendritic cells (DC) subset. Observations highlighted that microchimerism was not necessarily associated with favourable clinical outcomes in the first year post-lung transplantation.
authors.affiliation: Department of Medicine, Monash University, Central Clinical School, Melbourne, Vic., Australia.
authors.forename: L C
authors.initials: LC
authors.lastname: Rowntree
authors.forename: J
authors.initials: J
authors.lastname: Bayliss
authors.forename: T H O
authors.initials: TH
authors.lastname: Nguyen
authors.forename: T C
authors.initials: TC
authors.lastname: Kotsimbos
authors.forename: N A
authors.initials: NA
authors.lastname: Mifsud
mesh_terms: D000328:Adult; D000368:Aged; D016175:B-Lymphocyte Subsets / Q000276:immunology; D001483:Base Sequence; D046528:Chimerism; D015331:Cohort Studies; D017931:DNA Primers / Q000235:genetics; D005260:Female; D059948:HLA-B44 Antigen / Q000235:genetics*; D006801:Humans; D007694:Killer Cells, Natural / Q000276:immunology; D016040:Lung Transplantation / Q000276:immunology* / Q000502:physiology; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000276:immunology; D012189:Retrospective Studies; D016176:T-Lymphocyte Subsets / Q000276:immunology; D014019:Tissue Donors; D014184:Transplantation, Homologous; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: D017931:DNA Primers; D059948:HLA-B44 Antigen