title: Nematicidal activity of allylisothiocyanate from horseradish (Armoracia rusticana) roots against Meloidogyne incognita.
abstract: In recent years, there has been a great development in the search for new natural pesticides for crop protection aiming a partial or total replacement of currently used chemical nematicides. Glucosinolate breakdown products are volatile and are therefore good candidates for nematodes fumigants. In this article, the methanol-aqueous extract (1:1, w/v) of horseradish (Armoracia rusticana) fresh roots (MAH) was in vitro tested for nematicidal activity against second stage (J2) Meloidogyne incognita. The EC50 of MAH after 3 days of J2 immersion in test solutions was 251 ± 46 mg/L. The chemical composition analysis of the extract carried out by the GC-MS technique showed that allylisothicyanate was the most abundant compound. This pure compound induced J2 paralysis with an EC50 of 52.6 ± 45.6 and 6.6 ± 3.4 mg/L after 1 h and 3 days of incubation. The use of LC-MS/MS showed for the first time that horseradish root is rich in polyphenols. The study of isothiocyanate degradation in soil showed that allylisothiocyanate was the most quickly degradable compound (half-life <10 min), whereas no significant differences in half-life time were noted between degradation in regular and autoclaved soil.
authors.affiliation: Department of Life and Environmental Sciences, University of Cagliari, Via Ospedale 72, 09124 Cagliari, Italy.
authors.forename: Nadhem
authors.initials: N
authors.lastname: Aissani
authors.forename: Paola
authors.initials: P
authors.lastname: Tedeschi
authors.forename: Annalisa
authors.initials: A
authors.lastname: Maietti
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Brandolini
authors.forename: Vincenzo Luigi
authors.initials: VL
authors.lastname: Garau
authors.forename: Pierluigi
authors.initials: P
authors.lastname: Caboni
mesh_terms: D000818:Animals; D000969:Antinematodal Agents / Q000494:pharmacology*; D031215:Armoracia / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D006207:Half-Life; D017879:Isothiocyanates / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry*; D012987:Soil / Q000737:chemistry; D014431:Tylenchoidea / Q000187:drug effects*
chemical_list: D000969:Antinematodal Agents; D017879:Isothiocyanates; D010936:Plant Extracts; D012987:Soil; C004471:allyl isothiocyanate