title: Measurement of ferritin and anti-ferritin autoantibodies in serum and colostrum of Holstein and Japanese Black cows.
abstract: Anti-ferritin autoantibody is a ferritin-binding protein commonly found in mammals; it is thought to form an immune complex with ferritin and thereby mediate the rapid clearance of circulating ferritin. The aim of this study is to determine concentrations of ferritin and anti-ferritin autoantibodies (immunoglobulin (Ig)M, IgG and IgA) in serum and colostrum of Holstein (H) and Japanese Black (JB) cows within 24 h of normal calving. Blood and colostrum samples were collected from cows of various ages (2-11 years) and calving number (1-8 live births). Mean ferritin concentrations were higher in colostrum than in serum for both breeds, and higher colostrum ferritin concentrations were found in H than JB cows. IgA antibodies in serum and colostrum from both breeds had negligible ferritin-binding activity. For both breeds, IgM and IgG antibodies had higher ferritin-binding activity in colostrum than in serum. There was a significant correlation between IgM and IgG ferritin-binding activities in serum and colostrum of H and JB cows. These results suggest that ferritin and IgM and IgG autoantibodies are actively transferred from the blood stream to the colostrum at prepartum or early lactation.
authors.affiliation: Laboratory of Veterinary Biochemistry, School of Veterinary Medicine, Kitasato University, Aomori, Japan.
authors.forename: Yoshiya
authors.initials: Y
authors.lastname: Kanno
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Ohtsuka
authors.forename: Yasunaga
authors.initials: Y
authors.lastname: Yoshikawa
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Watanabe
authors.forename: Koichi
authors.initials: K
authors.lastname: Orino
mesh_terms: D000818:Animals; D001323:Autoantibodies / Q000097:blood* / Q000378:metabolism*; D002417:Cattle; D003126:Colostrum / Q000276:immunology* / Q000378:metabolism*; D005260:Female; D005293:Ferritins / Q000097:blood* / Q000276:immunology* / Q000378:metabolism; D007070:Immunoglobulin A / Q000276:immunology / Q000378:metabolism; D007074:Immunoglobulin G / Q000276:immunology / Q000378:metabolism; D007075:Immunoglobulin M / Q000276:immunology / Q000378:metabolism; D007774:Lactation / Q000276:immunology / Q000378:metabolism; D011247:Pregnancy; D011485:Protein Binding
chemical_list: D001323:Autoantibodies; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D005293:Ferritins
keywords: Holstein; Japanese Black; anti-ferritin autoantibody; colostrum; ferritin