title: Upregulated hPuf-A promotes breast cancer tumorigenesis.
abstract: hPuf-A is a member of RNA-binding PUF family that regulates mRNA translation. Redistribution of hPuf-A from the nucleolus to the nucleoplasm upon genotoxic stress modulates the poly(ADP-ribosyl)ation activity of PARP-1. Here, we report a novel function of hPuf-A involved in promoting breast cancer progression. Immunohistochemical studies showed higher expression levels of hPuf-A in stage I, II, III, and IV breast cancer specimens in contrast with those of hPuf-A in ductal carcinoma in situ. The presence of hPuf-A is highly associated with colony formation capacities in breast cancer T47D and MDA-MB-231 cells. Xenograft growth of hPuf-A-silenced and hPuf-A overexpressing MDA-MB-231 cells in nude mice was substantially in concert with colony formation capacities. This promoting effect of hPuf-A in tumorigenesis might be correlated with the regulation of its associated mRNAs, such as RbAp48 and DDX3. Collectively, hPuf-A may have diagnostic values in breast cancer progression.
authors.affiliation: Department of Physiology, Mackay Memorial Hospital, Taipei, Taiwan, pitcher.5552@ms2.mmh.org.tw.
authors.forename: Chi-Chen
authors.initials: CC
authors.lastname: Fan
authors.forename: Liek-Yeow
authors.initials: LY
authors.lastname: Lee
authors.forename: Ming-Ying
authors.initials: MY
authors.lastname: Yu
authors.forename: Chi-Yuan
authors.initials: CY
authors.lastname: Tzen
authors.forename: Chi
authors.initials: C
authors.lastname: Chou
authors.forename: Mau-Sun
authors.initials: MS
authors.lastname: Chang
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D063646:Carcinogenesis / Q000378:metabolism* / Q000473:pathology; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000378:metabolism* / Q000556:secondary; D045744:Cell Line, Tumor; D005260:Female; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism* / Q000556:secondary; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D015778:Minor Histocompatibility Antigens; D009367:Neoplasm Staging; D009368:Neoplasm Transplantation; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D047368:Tumor Burden; D015854:Up-Regulation
chemical_list: D015778:Minor Histocompatibility Antigens; C479948:PUM3 protein, human; D016601:RNA-Binding Proteins