title: Hardystonite improves biocompatibility and strength of electrospun polycaprolactone nanofibers over hydroxyapatite: a comparative study.
abstract: The aim of this study was to compare physico-chemical and biological properties of hydroxyapatite (HA) and hardystonite (HS) based composite scaffolds. Hardystonite (Ca2ZnSi2O7) powders were synthesized by a sol-gel method while polycaprolactone-hardystonite (PCL-HS) and polycaprolactone-hydroxyapatite (PCL-HA) were fabricated in nanofibrous form by electrospinning. The physico-chemical and biological properties such as tensile strength, cell proliferation, cell infiltration and alkaline phosphatase activity were determined on both kinds of scaffolds. We found that PCL-HS scaffolds had better mechanical strength compared to PCL-HA scaffolds. Addition of HA and HS particles to PCL did not show any inhibitory effect on blood biocompatibility of scaffolds when assessed by hemolysis assay. The in vitro cellular behavior was evaluated by growing murine adipose-tissue-derived stem cells (mE-ASCs) over the scaffolds. Enhanced cell proliferation and improved cellular infiltrations on PCL-HS scaffolds were observed when compared to HA containing scaffolds. PCL-HS scaffolds exhibited a significant increase in alkaline phosphatase (ALP) activity and better mineralization of the matrix in comparison to PCL-HA scaffolds. These results clearly demonstrate the stimulatory role of Zn and Si present in HS based composite scaffolds, suggesting their potential application for bone tissue engineering.
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai, 400076, India.
authors.forename: Amit K
authors.initials: AK
authors.lastname: Jaiswal
authors.forename: Hemlata
authors.initials: H
authors.lastname: Chhabra
authors.forename: Sachin S
authors.initials: SS
authors.lastname: Kadam
authors.forename: Kishore
authors.initials: K
authors.lastname: Londhe
authors.forename: Vivek P
authors.initials: VP
authors.lastname: Soni
authors.forename: Jayesh R
authors.initials: JR
authors.lastname: Bellare
mesh_terms: D001672:Biocompatible Materials; D017886:Durapatite / Q000737:chemistry*; D057139:Nanofibers / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D017886:Durapatite