title: Grafting of phosphorylcholine functional groups on polycarbonate urethane surface for resisting platelet adhesion.
abstract: In order to improve the resistance of platelet adhesion on material surface, 2-methacryloyloxyethyl phosphorylcholine (MPC) was grafted onto polycarbonate urethane (PCU) surface via Michael reaction to create biomimetic structure. After introducing primary amine groups via coupling tris(2-aminoethyl)amine (TAEA) onto the polymer surface, the double bond of MPC reacted with the amino group to obtain MPC modified PCU. The modified surface was characterized by Fourier transform infrared (FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS). The results verified that MPC was grafted onto PCU surface by Michael reaction method. The MPC grafted PCU surface had a low water contact angle and a high water uptake. This means that the hydrophilic PC functional groups improved the surface hydrophilicity significantly. In addition, surface morphology of MPC grafted PCU film was imaged by atomic force microscope (AFM). The results showed that the grafted surface was rougher than the blank PCU surface. In addition, platelet adhesion study was evaluated by scanning electron microscopy (SEM) observation. The PCU films after treated with platelet-rich plasma demonstrated that much fewer platelets adhered to the MPC-grafted PCU surface than to the blank PCU surface. The antithrombogenicity of the MPC-grafted PCU surface was determined by the activated partial thromboplastin time (APTT). The result suggested that the MPC modified PCU may have potential application as biomaterials in blood-contacting and some subcutaneously implanted devices.
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Weijin Road 92, Tianjin 300072, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Gao
authors.forename: Yakai
authors.initials: Y
authors.lastname: Feng
authors.forename: Jian
authors.initials: J
authors.lastname: Lu
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Miao
authors.initials: M
authors.lastname: Zhao
authors.forename: Changcan
authors.initials: C
authors.lastname: Shi
authors.forename: Musammir
authors.initials: M
authors.lastname: Khan
authors.forename: Jintang
authors.initials: J
authors.lastname: Guo
mesh_terms: D001792:Blood Platelets / Q000166:cytology*; D002448:Cell Adhesion; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D010767:Phosphorylcholine / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D011076:Polycarboxylate Cement / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D014520:Urethane / Q000737:chemistry*
chemical_list: D011076:Polycarboxylate Cement; D010767:Phosphorylcholine; C005506:polycarbonate; D014520:Urethane