title: Development and validation of a robust automated analysis of plasma phospholipid fatty acids for metabolic phenotyping of large epidemiological studies.
abstract: A fully automated, high-throughput method was developed to profile the fatty acids of phospholipids from human plasma samples for application to a large epidemiological sample set (n > 25,000). We report here on the data obtained for the quality-control materials used with the first 860 batches, and the validation process used. The method consists of two robotic systems combined with gas chromatography, performing lipid extraction, phospholipid isolation, hydrolysis and derivatization to fatty-acid methyl esters, and on-line analysis. This is the first report showing that fatty-acid profiling is an achievable strategy for metabolic phenotyping in very large epidemiological and genetic studies.
authors.affiliation: Medical Research Council Human Nutrition Research, Elsie Widdowson Laboratory, 120 Fulbourn Road, Cambridge, CB1 9NL, UK.
authors.forename: Laura Yun
authors.initials: LY
authors.lastname: Wang
authors.affiliation: Medical Research Council Human Nutrition Research, Elsie Widdowson Laboratory, 120 Fulbourn Road, Cambridge, CB1 9NL, UK.
authors.forename: Keith
authors.initials: K
authors.lastname: Summerhill
authors.affiliation: Medical Research Council Human Nutrition Research, Elsie Widdowson Laboratory, 120 Fulbourn Road, Cambridge, CB1 9NL, UK.
authors.forename: Carmen
authors.initials: C
authors.lastname: Rodriguez-Canas
authors.affiliation: Medical Research Council Human Nutrition Research, Elsie Widdowson Laboratory, 120 Fulbourn Road, Cambridge, CB1 9NL, UK.
authors.forename: Ian
authors.initials: I
authors.lastname: Mather
authors.affiliation: MRC Epidemiology Unit, Institute of Metabolic Science, Box 285, Addenbrooke's Hospital, Hills Road, Cambridge, CB2 0QQ UK.
authors.forename: Pinal
authors.initials: P
authors.lastname: Patel
authors.affiliation: Medical Research Council Human Nutrition Research, Elsie Widdowson Laboratory, 120 Fulbourn Road, Cambridge, CB1 9NL, UK.
authors.forename: Michael
authors.initials: M
authors.lastname: Eiden
authors.affiliation: Medical Research Council Human Nutrition Research, Elsie Widdowson Laboratory, 120 Fulbourn Road, Cambridge, CB1 9NL, UK.
authors.forename: Stephen
authors.initials: S
authors.lastname: Young
authors.affiliation: MRC Epidemiology Unit, Institute of Metabolic Science, Box 285, Addenbrooke's Hospital, Hills Road, Cambridge, CB2 0QQ UK.
authors.forename: Nita G
authors.initials: NG
authors.lastname: Forouhi
authors.affiliation: Medical Research Council Human Nutrition Research, Elsie Widdowson Laboratory, 120 Fulbourn Road, Cambridge, CB1 9NL, UK.
authors.forename: Albert
authors.initials: A
authors.lastname: Koulman