title: Expression of NFkB1, GADD45A and JNK1 in salivary gland carcinomas of different histotypes.
abstract: The class of salivary gland tumours is very heterogenous, both in a histopathological and clinical sense. Since they are uncommon lesions, their clinical management is still problematic. Molecular mechanisms underlying the development of these cancer types may be fundamental for the diagnosis, treatment and prognosis of this disease. In this study, the gene expression of nuclear factor-kappa B (NKkB1/p65), c-Jun N-terminal kinase (JNK1) and growth arrest and DNA damage (GADD45A), which all play an important role in inflammatory and cell survival mechanisms, was assessed in benign and malignant neoplasms of the salivary gland. The absolute mRNA content of paraffin embedded samples of salivary gland cancer was determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) using specific primers for NFkB1, GADD45A and JNK1. Expression values (relative to HPRT) were statistically evaluated. Among the detected alterations in gene expression, the only difference reaching statistical significance was in the case of NFkB1 in adenocystic carcinomas (p=0.05). Given the importance of these signalling mechanisms in the biology of tumorigenesis, these results may be implemented in further research and these genes might become targets for innovative diagnostic and therapeutic strategies.
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Faculty of Medicine, University of Pécs, Szigeti u. 12., H-7624, Pécs, Hungary.
authors.forename: Gyula
authors.initials: G
authors.lastname: Gobel
authors.forename: István
authors.initials: I
authors.lastname: Szanyi
authors.forename: Péter
authors.initials: P
authors.lastname: Révész
authors.forename: Miklós
authors.initials: M
authors.lastname: Bauer
authors.forename: Imre
authors.initials: I
authors.lastname: Gerlinger
authors.forename: Árpád
authors.initials: Á
authors.lastname: Németh
authors.forename: István
authors.initials: I
authors.lastname: Ember
authors.forename: Katalin
authors.initials: K
authors.lastname: Gocze
authors.forename: Katalin
authors.initials: K
authors.lastname: Gombos
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D018797:Cell Cycle Proteins / Q000096:biosynthesis* / Q000235:genetics; D004249:DNA Damage; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D008875:Middle Aged; D048055:Mitogen-Activated Protein Kinase 8 / Q000096:biosynthesis* / Q000235:genetics; D052002:NF-kappa B p50 Subunit / Q000096:biosynthesis* / Q000235:genetics; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000235:genetics; D012468:Salivary Gland Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D014162:Transfection; D000097642:GADD45 Proteins
chemical_list: D018797:Cell Cycle Proteins; D048055:Mitogen-Activated Protein Kinase 8; D052002:NF-kappa B p50 Subunit; D009687:Nuclear Proteins; C439070:GADD45A protein, human; D000097642:GADD45 Proteins; C489548:NFKB1 protein, human