title: Exploring the relationship between 5'AMP-activated protein kinase and markers related to type 2 diabetes mellitus.
abstract: The importance of 5'AMP-activated protein kinase (AMPK) in regulating glucose and fatty acid metabolism is increasing. Thus, it is regarded as a new pharmacological target for treatment of obesity, insulin resistance and type 2 diabetes mellitus (T2DM). In order to explore the relationships between AMPK and diabetes mellitus, urines samples from four groups of C57 mice, i.e., the normal male and female C57 mice, female C57-AMPK gene knocked-out mice, and male C57-AMPK gene knocked-out mice, were studied by coupling GC/MS with a powerful machine learning method, random forest. The experimentation has been designed as two steps: firstly, the normal male and female mice were compared with male and female C57-AMPK gene knocked-out mice, respectively; then the differences between male C57-AMPK gene knocked-out mice and female C57-AMPK gene knocked-out mice were further detected. Finally, not only the differences between the normal C57 mice and C57-AMPK gene knocked-out mice were observed, but also the gender-related metabolites differences of the C57-AMPK gene knocked-out mice were obviously visualized. The results obtained with this research demonstrate that combining GC/MS profiling with random forest is a useful approach to analyze metabolites and to screen the potential biomarkers for exploring the relationships between AMPK and diabetes mellitus.
authors.affiliation: Research Center of Modernization of Traditional Chinese Medicines, Central South University, Changsha 410083, PR China.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Huang
authors.forename: Rui-Hua
authors.initials: RH
authors.lastname: He
authors.forename: Lun-Zhao
authors.initials: LZ
authors.lastname: Yi
authors.forename: Hua-Lin
authors.initials: HL
authors.lastname: Xie
authors.forename: Dong-sheng
authors.initials: DS
authors.lastname: Cao
authors.forename: Yi-Zeng
authors.initials: YZ
authors.lastname: Liang
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D003924:Diabetes Mellitus, Type 2 / Q000201:enzymology*; D005260:Female; D055785:Gene Knockdown Techniques; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D015415:Biomarkers; D055372:AMP-Activated Protein Kinases