title: Emulsified isoflurane postconditioning produces cardioprotection against myocardial ischemia-reperfusion injury in rats.
abstract: Emulsified isoflurane (EIso) preconditioning can induce cardioprotection. We investigated whether EIso application after ischemia protects hearts against reperfusion injury and whether it is mediated by the inhibition of apoptosis. Rats were subjected to 30-min coronary occlusion followed by 180-min reperfusion. At the onset of reperfusion, rats were intravenously administered saline (sham, control group), 30 % intralipid (IL group) or 2 ml kg(-1) EIso (EIso group) for 30 min. After reperfusion, infarct sizes, myocardial apoptosis and expression of Bcl-2, Bax and caspase-3 proteins were determined. Hemodynamic parameters were not different among groups. Compared with control and intralipid group, EIso limited infarct size, inhibited apoptosis, increased the expression of Bcl-2, decreased the expression of Bax, cleaved caspase-3, and enhanced Bcl-2/Bax ratio. EIso protects hearts against reperfusion injury when administered at the onset of reperfusion, which may be mediated by the inhibition of apoptosis via modulation of the expression of pro- and anti-apoptotic proteins.
authors.affiliation: Department of Anesthesiology and Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu 610041, People's Republic of China.
authors.forename: Zhao-Yang
authors.initials: ZY
authors.lastname: Hu
authors.forename: Geoffrey W
authors.initials: GW
authors.lastname: Abbott
authors.forename: Ya-Dong
authors.initials: YD
authors.lastname: Fang
authors.forename: Yue-Sheng
authors.initials: YS
authors.lastname: Huang
authors.forename: Jin
authors.initials: J
authors.lastname: Liu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D004655:Emulsions / Q000008:administration & dosage; D057775:Ischemic Postconditioning / Q000379:methods*; D007530:Isoflurane / Q000008:administration & dosage*; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000517:prevention & control*; D010743:Phospholipids / Q000008:administration & dosage; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D013024:Soybean Oil / Q000008:administration & dosage; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis
chemical_list: D004655:Emulsions; D010743:Phospholipids; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; C545823:soybean oil, phospholipid emulsion; D013024:Soybean Oil; D007530:Isoflurane