title: Topical tacrolimus does not negatively impact acute skin wound healing.
abstract: Despite the increasing use of topical tacrolimus, there is little information about its effect on skin wound healing. To determine effects on acute cutaneous wound healing, two full-thickness skin wounds were imparted on the backs of 45 hairless mice, which were then divided into vehicle-, topical tacrolimus- and topical steroid-treated group. Each drug was topically applied once daily. The wound area was assessed by using dermoscopic images every two days after wounding. At 3, 7 and 11 days after wounding, 10 wounds in each group were collected for semi-quantitative analysis of histological features including re-epithelialization, polymorphonuclear leucocytes, fibroblasts and collagen. We also checked the mRNA expression levels of EGF, TGF-β, TNF-α and IL-1α. While topical application of clobetasol propionate was found to delay re-epithelialization and infiltration of polymorphonuclear leucocyte, topical treatment with tacrolimus showed patterns similar to that of the vehicle. In the tacrolimus-treated group, mRNA expression levels of IL-1α and TGF-β were slightly decreased, while the others were similar with the vehicle-treated group. Unlike steroid, topical tacrolimus, therefore, did not disturb the wound healing process in a murine skin wound model.
authors.forename: Sun
authors.initials: S
authors.lastname: Namkoong
authors.forename: Jimin
authors.initials: J
authors.lastname: Chung
authors.forename: Jiyeon
authors.initials: J
authors.lastname: Yoo
authors.forename: Minyoung
authors.initials: M
authors.lastname: Jung
authors.forename: Jiwon
authors.initials: J
authors.lastname: Gye
authors.forename: Ji Seok
authors.initials: JS
authors.lastname: Kim
authors.forename: Jee Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Sung Ku
authors.initials: SK
authors.lastname: Ahn
authors.forename: Byung Cheol
authors.initials: BC
authors.lastname: Park
authors.forename: Myung Hwa
authors.initials: MH
authors.lastname: Kim
authors.forename: Seung Phil
authors.initials: SP
authors.lastname: Hong
mesh_terms: D000208:Acute Disease; D000287:Administration, Topical; D000818:Animals; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D051379:Mice; D008812:Mice, Hairless; D012867:Skin / Q000187:drug effects / Q000293:injuries*; D013256:Steroids / Q000494:pharmacology; D016559:Tacrolimus / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D007166:Immunosuppressive Agents; D013256:Steroids; D016559:Tacrolimus