title: Coupled expression of Cu/Zn-superoxide dismutase and catalase in cassava improves tolerance against cold and drought stresses.
abstract: Recently we reported that the joint expression of cassava Cu/Zn superoxide dismutase (MeCu/ZnSOD) and catalase (MeCAT1) prolonged the shelf life of cassava storage-roots by the stabilization of reactive oxygen species (ROS) homeostasis after harvest. Since oxidative damage is a major feature of plants exposed to environmental stresses, transgenic cassava showing increased expression of the cytosolic MeCu/ZnSOD and the peroxisomal MeCAT1 should have improved resistance against other abiotic stresses. After cold treatment, the transgenic cassava maintained higher SOD and CAT activities and lower malendialdehyde content than those of wild type plants (WT). Detached leaves of transgenic cassava also showed slower transpirational water loss than those of WT. When plants were not watered for 30 d, transgenic lines exhibited a significant increase in water retention ability, accumulated 13% more proline and 12% less malendialdehyde than WT's, and showed enhanced activity of SOD and CAT. These results imply that manipulation of the antioxidative mechanism allows the development of staple crops with improved tolerance to abiotic stresses.
authors.affiliation: 1 National Key Laboratory of Plant Molecular Genetics and National Center for Plant Gene Research; Institute of Plant Physiology and Ecology; Shanghai Institutes for Biological Sciences; Chinese Academy of Sciences; Shanghai, PR China.
authors.forename: Jia
authors.initials: J
authors.lastname: Xu
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Duan
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: John R
authors.initials: JR
authors.lastname: Beeching
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
mesh_terms: D000064:Acclimatization; D002374:Catalase / Q000378:metabolism*; D003080:Cold Temperature; D055864:Droughts; D002365:Manihot / Q000201:enzymology*; D013312:Stress, Physiological; D013482:Superoxide Dismutase / Q000378:metabolism*; D014867:Water / Q000502:physiology*
chemical_list: D014867:Water; D002374:Catalase; D013482:Superoxide Dismutase
keywords: ROS scavenging; abiotic stress resistance; cassava; catalase; superoxide dismutase