title: Unraveling chemokine and chemokine receptor expression patterns using genetically engineered mice.
abstract: Over the past 25 years, genetically engineered mouse models have become an integral and invaluable research tool to develop our understanding of mammalian physiology and pathology. This unit describes methods for generating transgenic mice, focusing on reporter animals relevant to chemokine receptor and ligand expression. Specifically, we describe the use of bacterial artificial chromosome (BAC) engineering and embryonic stem cell manipulation to generate "knock in" and transgenic mice.
authors.affiliation: The Rayne Institute, University College London, London, UK.
authors.forename: Simon
authors.initials: S
authors.lastname: Yona
authors.forename: Ki-Wook
authors.initials: KW
authors.lastname: Kim
authors.forename: Rebecca
authors.initials: R
authors.lastname: Haffner
authors.forename: Steffen
authors.initials: S
authors.lastname: Jung
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D018925:Chemokines / Q000235:genetics / Q000378:metabolism*; D022202:Chromosomes, Artificial, Bacterial; D053595:Embryonic Stem Cells / Q000502:physiology; D005786:Gene Expression Regulation; D055879:Gene Knock-In Techniques; D051379:Mice; D008822:Mice, Transgenic; D019707:Receptors, Chemokine / Q000235:genetics / Q000378:metabolism*
chemical_list: D018925:Chemokines; D019707:Receptors, Chemokine