title: Development of a MALDI-TOF MS strategy for the high-throughput analysis of biomarkers: on-target aptamer immobilization and laser-accelerated proteolysis.
abstract: An aptamer-based strategy was developed for the high-throughput analysis of protein biomarkers, such as lysozyme, by on-target MALDI-TOF MS. The aptamers were immobilized on the target plate through formation of covalent bonds with a stable and porous gold layer. An infrared laser was subsequently applied for fast proteolysis. High sensitivities were observed both in standard solutions and human urine.
authors.affiliation: Department of Chemistry and Institute of Biomedical Science, Fudan University, 220 Handan Road, Shanghai, 200433, China.
authors.forename: Xueyang
authors.initials: X
authors.lastname: Zhang
authors.forename: Shaochun
authors.initials: S
authors.lastname: Zhu
authors.forename: Ya
authors.initials: Y
authors.lastname: Xiong
authors.forename: Chunhui
authors.initials: C
authors.lastname: Deng
authors.forename: Xiangmin
authors.initials: X
authors.lastname: Zhang
mesh_terms: D052158:Aptamers, Peptide; D015415:Biomarkers; D006046:Gold / Q000737:chemistry*; D057166:High-Throughput Screening Assays; D006801:Humans; D059748:Proteolysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D052158:Aptamers, Peptide; D015415:Biomarkers; D006046:Gold