title: Inkjet-printed polyaniline patterns for exocytosed molecule detection from live cells.
abstract: Polyaniline (PANi) patterns on flexible substrate are fabricated for biomolecule detection from live cells. PANi patterns are prepared by inkjet printing on polyethylene terephthalate film. Subsequently, arginine-glycine-aspartate (RGD) peptide is immobilized on the PANi pattern to selectively adhere cells. Rat pheochromocytoma PC12 cells are cultured on the RGD-immobilized PANi pattern, and patterned with high selectivity and growth. Additionally, the cells show focal adhesion on the RGD-immobilized PANi pattern, which are confirmed with vinculin staining and scanning electron microscopic images. To monitor dynamic biomolecular release from PC12 cells, RGD-immobilized PANi pattern is used for a real-time electrical signal detector. RGD-immobilized PANi patterning and sensing system represents outstanding ability to translate and amplify exocytosis molecules into a detectable signal as a transducer.
authors.affiliation: World Class University (WCU) program of Chemical Convergence for Energy & Environment (C2E2), School of Chemical and Biological Engineering, Seoul National University, 599 Gwanangro, Gwanak-gu, Seoul 151-742, Korea.
authors.forename: Wan-Kyu
authors.initials: WK
authors.lastname: Oh
authors.forename: Sojin
authors.initials: S
authors.lastname: Kim
authors.forename: Kyoung-Hwan
authors.initials: KH
authors.lastname: Shin
authors.forename: Yongjin
authors.initials: Y
authors.lastname: Jang
authors.forename: Moonjung
authors.initials: M
authors.lastname: Choi
authors.forename: Jyongsik
authors.initials: J
authors.lastname: Jang
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry*; D000818:Animals; D005089:Exocytosis; D022001:Focal Adhesions; D016716:PC12 Cells; D051381:Rats
chemical_list: D000814:Aniline Compounds; C416807:polyaniline