title: [Peripheral blood monocyte hepcidin in patients with multiple myeloma is associated with anemia of chronic disease].
abstract: Disorders of iron utilization caused by abnormal elevation of hepcidin levels are the main mechanism of anemia of chronic disease. Hepcidin is mainly produced by the liver. Recently it has been found that monocytes are another source of hepcidin. The increased hepcidin in serum and urine of multiple myeloma patients may be one cause of anemia of chronic disease (ACD). However it is unclear whether the peripheral blood monocyte hepcidin is involved in the pathogenesis of anemia of chronic disease. This study was purposed to investigate the role of monocyte hepcidin in multiple myeloma patients with anemia of chronic disease. The clinical data and peripheral venous blood of multiple myeloma patients were collected.Serum concentration of IL-6 and TNF-α was detected by ELISA. Peripheral blood monocytes were isolated by CD14(+) magnetic beads. Hepcidin, IL-6 and TNF-α mRNA of monocytes were detected by real time quantitative PCR. The results showed that the expression level of monocyte hepcidin mRNA in myeloma patients was higher than that in normal controls. In untreated patients, the expression level of monocyte hepcidin mRNA was negatively correlated with hemoglobin, and positively correlated with serum ferritin and IL-6 levels, but unrelated with TNF-α levels.It is concluded that the increased monocyte hepcidin levels in multiple myeloma patients may play an etiologic role in ACD.
authors.affiliation: Department of Hematology, Chinese Academy of Medical Sciences, Beijing, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Han
authors.forename: Dao-Bin
authors.initials: DB
authors.lastname: Zhou
authors.forename: Ming-Hui
authors.initials: MH
authors.lastname: Duan
authors.forename: Xuan
authors.initials: X
authors.lastname: Wang
authors.forename: Jie-Ping
authors.initials: JP
authors.lastname: Zhang
authors.forename: Yong-Qiang
authors.initials: YQ
authors.lastname: Zhao
authors.forename: Ti
authors.initials: T
authors.lastname: Shen
authors.forename: Yong-Ji
authors.initials: YJ
authors.lastname: Wu
mesh_terms: D000328:Adult; D000368:Aged; D000740:Anemia / Q000209:etiology*; D016022:Case-Control Studies; D002908:Chronic Disease; D005260:Female; D005293:Ferritins / Q000097:blood; D064451:Hepcidins / Q000097:blood*; D006801:Humans; D015850:Interleukin-6 / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism; D009101:Multiple Myeloma / Q000097:blood* / Q000150:complications; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D064451:Hepcidins; C508600:IL6 protein, human; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D005293:Ferritins