title: [The application of cation exchange resins in the separation of total flavonoids and alkaloids from seed embryo of Nelumbo nucifera].
abstract: OBJECTIVE
  To explore the optimal separation condition of the total flavonoids and alkaloids from seed embryo of Nelumbo nucifera.
  
  
  METHODS
  Types of cation exchange resins, concentration of extract liquid, solution volume, velocity of adsorption and eluent were evaluated in separating efficiency with measuring the adsorption ratio and eluent ratio.
  
  
  RESULTS
  D152 resin was finally chosen. After enrichment and purification with it, the purity of flavonoids and alkaloids were significantly increased by 27.08 and 7.86 times, respectively.
  
  
  CONCLUSIONS
  This method is simple, feasible and suitable for the separation of total flavonoids and alkaloids from seed embryo of Nelumbo nucifera.
authors.affiliation: Xiangya Hosipital, Central South University, Changsha 410008, China. xtcj2003@163.com
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Shao
authors.initials: S
authors.lastname: Liu
authors.forename: Xin-Zhong
authors.initials: XZ
authors.lastname: Li
mesh_terms: D000327:Adsorption; D000470:Alkaloids / Q000032:analysis / Q000302:isolation & purification*; D002411:Cation Exchange Resins / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol / Q000737:chemistry; D005419:Flavonoids / Q000032:analysis / Q000302:isolation & purification*; D031653:Nelumbo / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods*
chemical_list: D000470:Alkaloids; D002411:Cation Exchange Resins; D005419:Flavonoids; D000431:Ethanol