title: Disparate proteome responses of pathogenic and nonpathogenic aspergilli to human serum measured by activity-based protein profiling (ABPP).
abstract: Aspergillus fumigatus is the primary pathogen causing the devastating pulmonary disease Invasive Aspergillosis in immunocompromised individuals. There is high genomic synteny between A. fumigatus and closely related rarely pathogenic Neosartorya fischeri and Aspergillus clavatus genomes. We applied activity-based protein profiling to compare unique or overexpressed activity-based probe-reactive proteins of all three fungi over time in minimal media growth and in response to human serum. We found 360 probe-reactive proteins exclusive to A. fumigatus, including known virulence associated proteins, and 13 proteins associated with stress response exclusive to A. fumigatus culture in serum. Though the fungi are highly orthologous, A. fumigatus has a significantly greater number of ABP-reactive proteins across varied biological process. Only 50% of expected orthologs of measured A. fumigatus reactive proteins were observed in N. fischeri and A. clavatus. Activity-based protein profiling identified a number of processes that were induced by human serum in A. fumigatus relative to N. fischeri and A. clavatus. These included actin organization and assembly, transport, and fatty acid, cell membrane, and cell wall synthesis. Additionally, signaling proteins regulating vegetative growth, conidiation, and cell wall integrity, required for appropriate cellular response to external stimuli, had higher activity-based probe-protein reaction over time in A. fumigatus and N. fisheri, but not in A. clavatus. Together, we show that measured proteins and physiological processes identified solely or significantly over-represented in A. fumigatus reveal a unique adaptive response to human protein not found in closely related, but rarely pathogenic aspergilli. These unique activity-based probe-protein responses to culture condition may reveal how A. fumigatus initiates pulmonary invasion leading to Invasive Aspergillosis.
authors.affiliation: Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99352, USA.
authors.forename: Susan D
authors.initials: SD
authors.lastname: Wiedner
authors.forename: Charles
authors.initials: C
authors.lastname: Ansong
authors.forename: Bobbie-Jo
authors.initials: BJ
authors.lastname: Webb-Robertson
authors.forename: LeeAnna M
authors.initials: LM
authors.lastname: Pederson
authors.forename: Suereta
authors.initials: S
authors.lastname: Fortuin
authors.forename: Beth A
authors.initials: BA
authors.lastname: Hofstad
authors.forename: Anil K
authors.initials: AK
authors.lastname: Shukla
authors.forename: Ellen A
authors.initials: EA
authors.lastname: Panisko
authors.forename: Richard D
authors.initials: RD
authors.lastname: Smith
authors.forename: Aaron T
authors.initials: AT
authors.lastname: Wright
mesh_terms: D001232:Aspergillus fumigatus / Q000254:growth & development / Q000378:metabolism* / Q000472:pathogenicity; D005656:Fungal Proteins / Q000378:metabolism*; D006801:Humans; D040081:Protein Array Analysis; D020543:Proteome; D044967:Serum; D014774:Virulence
chemical_list: D005656:Fungal Proteins; D020543:Proteome