title: Physical mapping integrated with syntenic analysis to characterize the gene space of the long arm of wheat chromosome 1A.
abstract: BACKGROUND
  Bread wheat (Triticum aestivum L.) is one of the most important crops worldwide and its production faces pressing challenges, the solution of which demands genome information. However, the large, highly repetitive hexaploid wheat genome has been considered intractable to standard sequencing approaches. Therefore the International Wheat Genome Sequencing Consortium (IWGSC) proposes to map and sequence the genome on a chromosome-by-chromosome basis.
  
  
  RESULTS
  We have constructed a physical map of the long arm of bread wheat chromosome 1A using chromosome-specific BAC libraries by High Information Content Fingerprinting (HICF). Two alternative methods (FPC and LTC) were used to assemble the fingerprints into a high-resolution physical map of the chromosome arm. A total of 365 molecular markers were added to the map, in addition to 1122 putative unique transcripts that were identified by microarray hybridization. The final map consists of 1180 FPC-based or 583 LTC-based contigs.
  
  
  CONCLUSIONS
  The physical map presented here marks an important step forward in mapping of hexaploid bread wheat. The map is orders of magnitude more detailed than previously available maps of this chromosome, and the assignment of over a thousand putative expressed gene sequences to specific map locations will greatly assist future functional studies. This map will be an essential tool for future sequencing of and positional cloning within chromosome 1A.
authors.affiliation: Faculty of Engineering and Natural Sciences, Sabanci University, Tuzla, Istanbul, Turkey.
authors.forename: Stuart J
authors.initials: SJ
authors.lastname: Lucas
authors.forename: Bala Anı
authors.initials: BA
authors.lastname: Akpınar
authors.forename: Melda
authors.initials: M
authors.lastname: Kantar
authors.forename: Zohar
authors.initials: Z
authors.lastname: Weinstein
authors.forename: Fatma
authors.initials: F
authors.lastname: Aydınoğlu
authors.forename: Jan
authors.initials: J
authors.lastname: Safář
authors.forename: Hana
authors.initials: H
authors.lastname: Simková
authors.forename: Zeev
authors.initials: Z
authors.lastname: Frenkel
authors.forename: Abraham
authors.initials: A
authors.lastname: Korol
authors.forename: Federica
authors.initials: F
authors.lastname: Magni
authors.forename: Federica
authors.initials: F
authors.lastname: Cattonaro
authors.forename: Sonia
authors.initials: S
authors.lastname: Vautrin
authors.forename: Arnaud
authors.initials: A
authors.lastname: Bellec
authors.forename: Hélène
authors.initials: H
authors.lastname: Bergès
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Doležel
authors.forename: Hikmet
authors.initials: H
authors.lastname: Budak
mesh_terms: D002874:Chromosome Mapping / Q000379:methods*; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D032461:Chromosomes, Plant / Q000235:genetics*; D014908:Triticum / Q000235:genetics*