title: Preparation and evaluation of (99m)Tc-DMSA lyophilized kit for renal imaging.
abstract: Dimercaptosuccinic acid (DMSA) has been evaluated and used with technetium 99m ((99m)Tc) in imaging of kidneys. DMSA lyophilized kits were prepared and radiolabelled with (99m)Tc. Paper and thin-layer chromatography have been employed using various eluent systems for the radiochemical analysis, percentage labeling and binding capacity of (99m)Tc-DMSA. Female albino rabbits were used for this study. Biological data obtained after intravenous injection of radiolabelled DMSA to female albino rabbits revealed 32.42% uptake and long retention time in the kidneys. On the basis of animal biodistribution data, it is suggested that DMSA when labeled with (99m)Tc is useful complex for renal imaging and can be successfully applied as a diagnostic tool in nuclear medicine. Clinical biodistribution and radiation dosimetry studies are planned in future.
authors.affiliation: Department of Pharmacy, University of Balochistan, Quetta, Pakistan. suj55@yahoo.com
authors.forename: Syed Umer
authors.initials: SU
authors.lastname: Jan
authors.forename: Hafiz Ghulam
authors.initials: HG
authors.lastname: Abbass
mesh_terms: D000818:Animals; D004355:Drug Stability; D005260:Female; D005612:Freeze Drying / Q000379:methods; D007668:Kidney / Q000000981:diagnostic imaging* / Q000378:metabolism; D011817:Rabbits; D011874:Radiometry / Q000379:methods; D011877:Radionuclide Imaging / Q000379:methods; D019275:Radiopharmaceuticals / Q000138:chemical synthesis / Q000737:chemistry* / Q000493:pharmacokinetics; D011933:Reagent Kits, Diagnostic; D019783:Technetium Tc 99m Dimercaptosuccinic Acid / Q000138:chemical synthesis / Q000737:chemistry* / Q000493:pharmacokinetics; D013696:Temperature
chemical_list: D019275:Radiopharmaceuticals; D011933:Reagent Kits, Diagnostic; D019783:Technetium Tc 99m Dimercaptosuccinic Acid