title: Association between polymorphisms in renin-angiotensin system genes and primary ovarian insufficiency in Korean women.
abstract: OBJECTIVE
  The purpose of this study was to evaluate the relationship between angiotensin-converting enzyme (ACE; insertion/deletion), angiotensinogen (AGT M235T), and angiotensin II type 1 receptor (AT1R 1166A>C) and the prevalence of primary ovarian insufficiency (POI) in Korean women.
  
  
  METHODS
  A total of 133 women with POI and 238 controls were genotyped for polymorphic sites in each gene using polymerase chain reaction-restriction fragment length polymorphism analysis.
  
  
  RESULTS
  ACE ID and ID + II variants occurred more frequently in women with POI than in controls (odds ratio [OR], 1.830; 95% CI, 1.040-3.221; P = 0.040; and OR, 1.797; 95% CI, 1.055-3.060; P = 0.031, respectively). The AT1R 1166AC genotype occurred more frequently in participants with POI than in controls (OR, 3.171; 95% CI, 1.562-6.436; P = 0.002). Comparing the combined genotype frequencies of ACE/AT1R revealed that the frequencies of ID/AA, ID/AC, and II/AC were higher in participants than in controls (OR, 1.916; 95% CI, 1.053-3.485; P = 0.043; OR, 3.544; 95% CI, 1.207-10.407; P = 0.036; and OR, 7.875; 95% CI, 2.224-27.881; P = 0.001, respectively). The TT/AC genotype for combined genotyping of AGT/AT1R was more frequently observed in the POI group than in the control group (OR, 3.472; 95% CI, 1.450-8.313; P = 0.006). In multifactor dimensionality reduction-based haplotype analysis, the I-T-C genotype of ACE/AGT/AT1R was a possible predisposing factor for POI (OR, 4.678; 95% CI, 1.721-12.717; P = 0.002).
  
  
  CONCLUSIONS
  This study demonstrates that polymorphisms in the renin-angiotensin system are related to the prevalence of POI. Thus, these renin-angiotensin system genes may serve as a novel marker for predicting the development of POI.
authors.affiliation: Department of Obstetrics and Gynecology, School of Medicine, College of Life Science, CHA University, Seongnam, South Korea.
authors.forename: Yong Wook
authors.initials: YW
authors.lastname: Jung
authors.forename: Young Joo
authors.initials: YJ
authors.lastname: Jeon
authors.forename: Hye Mi
authors.initials: HM
authors.lastname: Park
authors.forename: Bo Eun
authors.initials: BE
authors.lastname: Lee
authors.forename: Hyungchul
authors.initials: H
authors.lastname: Rah
authors.forename: Woo Sik
authors.initials: WS
authors.lastname: Lee
authors.forename: Tae Ki
authors.initials: TK
authors.lastname: Yoon
authors.forename: Nam Keun
authors.initials: NK
authors.lastname: Kim
mesh_terms: D000328:Adult; D000808:Angiotensinogen / Q000235:genetics; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D005838:Genotype; D006801:Humans; D054643:INDEL Mutation; D007703:Peptidyl-Dipeptidase A / Q000235:genetics; D011110:Polymorphism, Genetic; D016649:Primary Ovarian Insufficiency / Q000235:genetics*; D044140:Receptor, Angiotensin, Type 1 / Q000235:genetics; D056910:Republic of Korea
chemical_list: D044140:Receptor, Angiotensin, Type 1; D000808:Angiotensinogen; D007703:Peptidyl-Dipeptidase A