title: Comment on "Drosophila dosage compensation involves enhanced Pol II recruitment to male X-linked promoters".
abstract: Conrad et al. (Reports, 10 August 2012, p. 742) reported a doubling of RNA polymerase II (Pol II) occupancy at X-linked promoters to support 5' recruitment as the key mechanism for dosage compensation in Drosophila. However, they employed an erroneous data-processing step, overestimating Pol II differences. Reanalysis of the data fails to support the authors' model for dosage compensation.
authors.affiliation: Center for Biomedical Informatics, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: F
authors.initials: F
authors.lastname: Ferrari
authors.forename: Y L
authors.initials: YL
authors.lastname: Jung
authors.forename: P V
authors.initials: PV
authors.lastname: Kharchenko
authors.forename: A
authors.initials: A
authors.lastname: Plachetka
authors.forename: A A
authors.initials: AA
authors.lastname: Alekseyenko
authors.forename: M I
authors.initials: MI
authors.lastname: Kuroda
authors.forename: P J
authors.initials: PJ
authors.lastname: Park
mesh_terms: D000818:Animals; D004257:DNA Polymerase II / Q000378:metabolism*; D004303:Dosage Compensation, Genetic; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins / Q000378:metabolism*; D005260:Female; D050172:Genes, X-Linked; D008297:Male; D011401:Promoter Regions, Genetic; D014960:X Chromosome / Q000235:genetics*
chemical_list: D029721:Drosophila Proteins; D004257:DNA Polymerase II