title: [Expression of endothelial nitric oxide synthase mRNA in human RBCs during storage].
abstract: This study was purposed to identify endothelial nitric oxide synthase (eNOS) mRNA in human RBCs during storage and to investigate the relationship of its changing profile and preservation time at 4°C. RT-PCR and gene sequencing were applied to identify eNOS-mRNA in banked RBC. Real time PCR was used to study the relationship of eNOS-mRNA expression and preservation time. The results showed that eNOS mRNA was detected in RBC. Compared with fresh RBC, the content of eNOS mRNA in RBC was 0.868 ± 0.119 stored for 1 week, which was 0.379 ± 0.289, 0.108 ± 0.134, 0.141 ± 0.141, 0.125 ± 0.12 stored for 2, 3, 4 and 5 weeks respectively. It is concluded that eNOS mRNA exists in human RBC and its content is decreasing gradually along with the prolongation of storage time in banked RBC. Stored for 3 weeks, the content of eNOS-mRNA remains to be at lower level of concentration in human RBC.
authors.affiliation: Blood Research Laboratory, Chengdu Blood Center, Sichuan Province, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Chen
authors.forename: Chang-Hua
authors.initials: CH
authors.lastname: Zhou
authors.forename: Ying
authors.initials: Y
authors.lastname: Hong
authors.forename: Tian-Xiang
authors.initials: TX
authors.lastname: Gong
mesh_terms: D001782:Blood Donors; D001793:Blood Preservation; D004912:Erythrocytes / Q000378:metabolism*; D006801:Humans; D052250:Nitric Oxide Synthase Type III / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; C496314:NOS3 protein, human; D052250:Nitric Oxide Synthase Type III