title: Chemokine receptor interactions with virus-like particles.
abstract: Virus-like particles (VLPs) presenting conformational envelope proteins on their surface represent an invaluable tool to study molecular interactions between viruses and cellular receptors/co-receptors, eliminating biological risks associated with working with live native viruses. The availability of target cells expressing specific chemokine receptors facilitates the dissection of specific interactions between human immunodeficiency virus (HIV) viral envelope proteins and these receptors in the laboratory. Here, we describe a method to evaluate HIV-VLP binding to cellular chemokine co-receptors, by carboxyfluorescein succinimidyl ester labeling and cellular uptake.
authors.affiliation: Molecular Biology and Viral Oncology, Department of Experimental Oncology, Istituto Nazionale Tumori "Fond G. Pascale", Naples, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Buonaguro
authors.forename: Maria
authors.initials: M
authors.lastname: Tagliamonte
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Visciano
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D005452:Fluoresceins / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods; D011485:Protein Binding; D019707:Receptors, Chemokine / Q000235:genetics / Q000378:metabolism*; D061987:Sf9 Cells; D013388:Succinimides / Q000378:metabolism; D013997:Time Factors; D014162:Transfection; D058425:Vaccines, Virus-Like Particle / Q000235:genetics / Q000378:metabolism*; D053586:Virus Internalization; D054299:env Gene Products, Human Immunodeficiency Virus / Q000235:genetics / Q000378:metabolism*
chemical_list: C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D005452:Fluoresceins; D005456:Fluorescent Dyes; D019707:Receptors, Chemokine; D013388:Succinimides; D058425:Vaccines, Virus-Like Particle; D054299:env Gene Products, Human Immunodeficiency Virus