title: Induction of a zearalenone degrading enzyme caused by the substrate and its derivatives.
abstract: The Fusarium toxin zearalenone (ZON) is very harmful to animal and man due to its estrogenic effect, immunotoxicity and genotoxicity. Therefore, it is of high importance to establish a system for the detoxification of ZON. In large screening programmes, only the mycoparasiteGliocladium roseum (DSM 62726) was found to be capable of detoxifying ZON, by not yet characterized enzyme(s). It is the only known microorganism hydrolyzing the lactonic bond within the macrocyclic ring system of ZON. The resulting products are less toxic because they loose their estrogenic capacity. The extent of toxin degradation is enhanced when enzyme production inG roseum is induced by the substrate ZON itself and its derivatives. ZON and its derivatives differ in the ability to induce enzyme production. This was investigated underin vitro conditions. Differences were found in the required amount of the inducing substances and time optimum of induction in order to get maximal degradation of ZON. The regulation and biochemical properties of the enzyme are to be characterized as a prerequisite to develop applications aimed at the detoxification of ZON in food and feeding-stuff. Our aim is to isolate the unknown enzyme which is capable of the detoxification of this mycotoxin.
authors.affiliation: Institute of Phytomedicine (360), University of Hohenheim, Otto-Sander-Str. 5, 70593, Stuttgart, Germany.
authors.forename: I
authors.initials: I
authors.lastname: Matthies
authors.forename: G
authors.initials: G
authors.lastname: Woerfel
authors.forename: P
authors.initials: P
authors.lastname: Karlovsky