title: Serum miR-19a expression correlates with worse prognosis of patients with non-small cell lung cancer.
abstract: OBJECTIVE
  The aim of this study was to investigate the expression levels of miR-19a in non-small cell lung cancer (NSCLC) tissue and serum, and to clarify the relationships of serum miR-19a expression with clinical factors and prognosis of NSCLC patients.
  
  
  METHODS
  Expression levels of miR-19a in 25 paired NSCLC, paracancerous tissues and serum, and sera from 103 controls and 201 NSCLC patients were respectively detected using real-time quantitative PCR.
  
  
  RESULTS
  Compared with the paracancerous tissue, miR-19a was overexpressed in NSCLC tissue (P = 0.006), and there was a strong correlation between expression levels of miR-19 in 25 paired sera and tissues (P = 0.001). Serum miR-19a expression in NSCLC patients was significantly upregulated compared with those in healthy individuals (P = 0.001). High serum miR-19a expression was significantly correlated with TNM stage and lymph node metastasis (P = 0.004 and 0.017, respectively). Survival analysis revealed that overall survival rate of patients with high serum miR-19a expression was significantly worse than those of patients with low serum miR-19a expression (hazard ratio = 1.438, 95% confidence interval 1.007-2.052, P = 0.046).
  
  
  CONCLUSIONS
  High serum miR-19a expression may be an independent poor prognostic factor for survival in NSCLC patients.
authors.affiliation: Department of Respiratory Medicine, Affiliated Hospital of Putian University, Putian, Fujian, China.
authors.forename: Qunying
authors.initials: Q
authors.lastname: Lin
authors.forename: Tingjian
authors.initials: T
authors.lastname: Chen
authors.forename: Qingyu
authors.initials: Q
authors.lastname: Lin
authors.forename: Guosheng
authors.initials: G
authors.lastname: Lin
authors.forename: Juan
authors.initials: J
authors.lastname: Lin
authors.forename: Guohuan
authors.initials: G
authors.lastname: Chen
authors.forename: Lijing
authors.initials: L
authors.lastname: Guo
mesh_terms: D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000097:blood*; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood* / Q000737:chemistry / Q000473:pathology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000097:blood* / Q000737:chemistry / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D035683:MicroRNAs / Q000032:analysis* / Q000097:blood*; D008875:Middle Aged; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D012189:Retrospective Studies; D012907:Smoking / Q000009:adverse effects
chemical_list: D014408:Biomarkers, Tumor; C549214:MIRN19A microRNA, human; D035683:MicroRNAs