title: Inhibitory effect of neuropilin-1 monoclonal antibody (NRP-1 MAb) on glioma tumor in mice.
abstract: This article reports the effect of Neuropilin-1 monoclonal antibody (NRP-1 MAb) on glioma cell lines in vitro and the effect on nude mice bearing glioma tumor (U87) in vivo. MTT, the scratch test and transwell test were used to evaluate the inhibitory effect of NRP-1 MAb on glioma cell lines. The tissue distribution and tumor targeting capability of NRP-1 MAb in U87 Xenografts nude mice was determined in Optical Imaging. Anti-glioma experiment was carried out by NRP-1 MAb on U87 Xenografts nude mice. NRP-1 MAb was showed to inhibit the proliferation, migration and invasion of glioma cells. NRP-1 MAb can specifically target the tumor in U87 Xenografts nude mice and reduce the proliferation activity of the tumor. The results of this study indicate that NRP-1 MAb can inhibit the proliferation, migration and metastasis of glioma cell lines and can target the glioma tumor to reduce tumor growth. Therefore, NRP-1 MAb may be an effective agent for glioma-targeted therapy.
authors.affiliation: Department of Neurosurgery, Zhongda Hospital Southeast University, Nanjing, 210009, China. neuro_clk@hotmail.com
authors.forename: Lukui
authors.initials: L
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Miao
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Tang
authors.forename: Haipeng
authors.initials: H
authors.lastname: Zhang
authors.forename: Shengyu
authors.initials: S
authors.lastname: Wang
authors.forename: Fanghong
authors.initials: F
authors.lastname: Luo
authors.forename: Jianghua
authors.initials: J
authors.lastname: Yan
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology / Q000627:therapeutic use*; D001932:Brain Neoplasms / Q000188:drug therapy* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D003952:Diagnostic Imaging; D005910:Glioma / Q000188:drug therapy* / Q000473:pathology; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D008856:Microscopy, Fluorescence; D009361:Neoplasm Invasiveness; D039942:Neuropilin-1 / Q000276:immunology*; D061848:Optical Imaging; D051381:Rats; D047368:Tumor Burden / Q000187:drug effects
chemical_list: D000911:Antibodies, Monoclonal; D039942:Neuropilin-1