title: Circovirus in tissues of dogs with vasculitis and hemorrhage.
abstract: We characterized the complete genome of a novel dog circovirus (DogCV) from the liver of a dog with severe hemorrhagic gastroenteritis, vasculitis, and granulomatous lymphadenitis. DogCV was detected by PCR in fecal samples from 19/168 (11.3%) dogs with diarrhea and 14/204 (6.9%) healthy dogs and in blood from 19/409 (3.3%) of dogs with thrombocytopenia and neutropenia, fever of unknown origin, or past tick bite. Co-infection with other canine pathogens was detected for 13/19 (68%) DogCV-positive dogs with diarrhea. DogCV capsid proteins from different dogs varied by up to 8%. In situ hybridization and transmission electron microscopy detected DogCV in the lymph nodes and spleens of 4 dogs with vascular compromise and histiocytic inflammation. The detection of a circovirus in tissues of dogs expands the known tropism of these viruses to a second mammalian host. Our results indicate that circovirus, alone or in co-infection with other pathogens, might contribute to illness and death in dogs.
authors.affiliation: Blood Systems Research Institute, and Department of Laboratory Medicine, University of California, San Francisco, California, USA.
authors.forename: Linlin
authors.initials: L
authors.lastname: Li
authors.forename: Sabrina
authors.initials: S
authors.lastname: McGraw
authors.forename: Kevin
authors.initials: K
authors.lastname: Zhu
authors.forename: Christian M
authors.initials: CM
authors.lastname: Leutenegger
authors.forename: Stanley L
authors.initials: SL
authors.lastname: Marks
authors.forename: Steven
authors.initials: S
authors.lastname: Kubiski
authors.forename: Patricia
authors.initials: P
authors.lastname: Gaffney
authors.forename: Florante N
authors.initials: FN
authors.lastname: Dela Cruz
authors.forename: Chunlin
authors.initials: C
authors.lastname: Wang
authors.forename: Eric
authors.initials: E
authors.lastname: Delwart
authors.forename: Patricia A
authors.initials: PA
authors.lastname: Pesavento
mesh_terms: D000818:Animals; D002140:California / Q000453:epidemiology; D018173:Circoviridae Infections / Q000150:complications / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004279:DNA, Viral / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D003967:Diarrhea / Q000150:complications / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D004283:Dog Diseases / Q000453:epidemiology* / Q000821:virology; D004285:Dogs; D005243:Feces / Q000821:virology; D005260:Female; D006471:Gastrointestinal Hemorrhage / Q000150:complications / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D016679:Genome, Viral; D006801:Humans; D017403:In Situ Hybridization; D008099:Liver / Q000473:pathology / Q000821:virology; D008198:Lymph Nodes / Q000473:pathology / Q000821:virology; D008297:Male; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D013154:Spleen / Q000473:pathology / Q000821:virology; D014657:Vasculitis / Q000150:complications / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology
chemical_list: D004279:DNA, Viral
keywords: canine vascular disease; circovirus; deep sequencing; dogs; granulomatous lymphadenitis; hemorrhagic gastroenteritis; necrotizing vasculitis; vasculitis; viruses