title: [The inhibition effect of protocatechuic acid on the mRNA expression of APP on M146L cell].
abstract: OBJECTIVE
  To investigate the effect of protocatechuic acid on the mRNA expression of APP in double transfected (human APP gene and presenlin-1 gene) Chinese hamster ovary (CHO) cells (M146L).
  
  
  METHODS
  Abeta42 overexpressing cell model was established in vitro by culturing Chinese hamster ovary cells stably expressing amyloid beta-protein precursor and mutant presenilin (M146L). The MTT assay was used to test cytotoxicity of protocatechuic acid, and reverse transcription polymerase chain reaction (RT-PCR) was carried out to determine the mRNA expression level of APP.
  
  
  RESULTS
  The MTT assay showed that protocatechuic acid at suitable concentrations didn't have cytotoxicity on M146L cell survival. Protocatechuic acid at the concentration of 0.25 mmol/L, 0.5 mmol/L and 1.0 mmol/L significantly inhibited the mRNA expression of APP.
  
  
  CONCLUSIONS
  The study suggests that the suitable dose of protocatechuic acid could inhibit the mRNA expression of APP in M146L cell,and its mechanism needs further study.
authors.affiliation: Ganzhou People's Hospital, Ganzhou 34100, China. xuexiaoyan816@sina.com
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Xue
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhang
authors.forename: Xiao-Wen
authors.initials: XW
authors.lastname: Zhou
authors.forename: Xing
authors.initials: X
authors.lastname: Zhou
authors.forename: Huan-Min
authors.initials: HM
authors.lastname: Luo
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism / Q000473:pathology*; D016229:Amyloid beta-Peptides / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D016564:Amyloid beta-Protein Precursor / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D029064:Araceae / Q000737:chemistry*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006224:Cricetinae; D004305:Dose-Response Relationship, Drug; D062385:Hydroxybenzoates / Q000008:administration & dosage / Q000494:pharmacology*; D053764:Presenilin-1 / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D014162:Transfection
chemical_list: D016229:Amyloid beta-Peptides; D016564:Amyloid beta-Protein Precursor; D062385:Hydroxybenzoates; D053764:Presenilin-1; D012333:RNA, Messenger; C009091:protocatechuic acid