title: Basophils are required for the induction of Th2 immunity to haptens and peptide antigens.
abstract: The relative contributions of basophils and dendritic cells in Th2 skewing to foreign antigen exposure remain unclear. Here we report the ability of basophils to induce Th2 polarization upon epicutaneous sensitization with different antigens using basophil conditionally depleted Bas TRECK transgenic mice. Basophils are responsible for Th2 skewing to haptens and peptide antigens, but not protein antigens in vivo. Consistent with this, basophils cannot take up or process ovalbumin protein in significant quantities, but present ovalbumin peptide to T cells for Th2 differentiation via major histocompatibility complex class II. Intriguingly, basophils promote Th2 skewing upon ovalbumin protein exposure in the presence of dendritic cells. Taken together, our results suggest that basophils alone are able to induce Th2 skewing with haptens and peptide antigens but require dendritic cells for the induction of Th2 for protein antigens upon epicutaneous immunization.
authors.affiliation: Department of Dermatology, Kyoto University Graduate School of Medicine, Kyoto 606-8507, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Otsuka
authors.forename: Saeko
authors.initials: S
authors.lastname: Nakajima
authors.forename: Masato
authors.initials: M
authors.lastname: Kubo
authors.forename: Gyohei
authors.initials: G
authors.lastname: Egawa
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Honda
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kitoh
authors.forename: Takashi
authors.initials: T
authors.lastname: Nomura
authors.forename: Sho
authors.initials: S
authors.lastname: Hanakawa
authors.forename: Catharina
authors.initials: C
authors.lastname: Sagita Moniaga
authors.forename: Bongju
authors.initials: B
authors.lastname: Kim
authors.forename: Satoshi
authors.initials: S
authors.lastname: Matsuoka
authors.forename: Takeshi
authors.initials: T
authors.lastname: Watanabe
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Miyachi
authors.forename: Kenji
authors.initials: K
authors.lastname: Kabashima
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000187:drug effects; D000941:Antigens / Q000276:immunology*; D001491:Basophils / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D002454:Cell Differentiation / Q000276:immunology; D004167:Diphtheria Toxin / Q000494:pharmacology; D004423:Ear / Q000473:pathology; D006241:Haptens / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D007109:Immunity / Q000187:drug effects / Q000276:immunology*; D007074:Immunoglobulin G / Q000378:metabolism; D007249:Inflammation / Q000276:immunology / Q000473:pathology; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008822:Mice, Transgenic; D010047:Ovalbumin / Q000276:immunology; D010455:Peptides / Q000276:immunology* / Q000494:pharmacology; D018418:Th2 Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology*
chemical_list: D000941:Antigens; D004167:Diphtheria Toxin; D006241:Haptens; D000949:Histocompatibility Antigens Class II; D007074:Immunoglobulin G; D010455:Peptides; D010047:Ovalbumin