title: [Protective effects of crude polysaccharides from Chroogomphus rutilus on SH-SY5Y cells impaired by MPP(+)].
abstract: The aim of the present study was to investigate the protective effects of crude polysaccharides from Chroogomphus rutilus on dopaminergic neurons impaired by MPP(+). SH-SY5Y cells were pretreated with crude polysaccharides (200, 400 and 800 μg/mL), and then MPP(+) was added to cell medium. After 48 h of incubation, MTT method was used to detect the survival rate of SH-SY5Y cells damaged by MPP(+). Annexin V-FITC staining and flow cytometry were used to detect apoptotic rate. The results showed that pretreating SH-SY5Y cells with crude polysaccharides (400 and 800 μg/mL) increased the survival rates, and reduced the apoptotic rates of SH-SY5Y cells. To rule out the possibility that crude polysaccharides may decrease actual concentration of MPP(+) by direct binding, we washed off crude polysaccharides before MPP(+) addition. Under this experimental condition, MTT results showed the survival rates of the SH-SY5Y cells were still significantly increased by 800 μg/mL crude polysaccharides pretreatment. These results suggest a protective effect of polysaccharides on the SH-SY5Y cells. Most of this protection is contributed by direct action of polysaccharides on the cells, not by binding with MPP(+). It is indicated that the crude polysaccharides from Chroogomphus rutilus can be developed as a potential drug for Parkinson's disease prevention and treatment in the future.
authors.affiliation: College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, China.
authors.forename: Xue-Qian
authors.initials: XQ
authors.lastname: Zhang
authors.forename: Yan-Qin
authors.initials: YQ
authors.lastname: Wang
authors.forename: Li-An
authors.initials: LA
authors.lastname: Wang
authors.forename: Zhen-Lon
authors.initials: ZL
authors.lastname: Guan
mesh_terms: D015655:1-Methyl-4-phenylpyridinium / Q000009:adverse effects*; D017209:Apoptosis / Q000187:drug effects; D001487:Basidiomycota / Q000737:chemistry*; D045744:Cell Line, Tumor; D059290:Dopaminergic Neurons / Q000187:drug effects*; D006801:Humans; D011134:Polysaccharides / Q000494:pharmacology*
chemical_list: D011134:Polysaccharides; D015655:1-Methyl-4-phenylpyridinium