title: Protective effects of dexmedetomidine in pneumoperitoneum-related ischaemia-reperfusion injury in rat ovarian tissue.
abstract: OBJECTIVE
  To determine the effects of dexmedetomidine on pneuomoperitoneum-related ischaemia-reperfusion (I/R) injury in rat ovarian tissue.
  
  
  METHODS
  Animals were randomized into three groups: Group S (n=8), no pneumoperitoneum; Group C (n=8), pneumoperitoneum; and Group D (n=8), 100μg intraperitoneal dexmedetomidine 30min before pneumoperitoneum. Ovarian tissue was collected from all rats 30min after desufflation, and fresh frozen for histological and biochemical evaluation.
  
  
  RESULTS
  Body weight was similar in all three groups (202.62±28.86, 211.00±14.45 and 212.87±15.71g in Groups S, D and C, respectively). The mean malondialdehyde level was higher in Group C than the other groups (p<0.03). When the histological samples of ovarian tissue were compared, vascular congestion, haemorrhage, follicular cell degeneration and infiltrative cell infiltration scores were higher in Group C compared with the other groups (p<0.05). Significantly lower scores for the histological parameters were found in Group D compared with Group C (p<0.05). Similar scores for follicular cell degeneration and inflammatory cell infiltration were found in Group D and Group S (p>0.05). Although vascular congestion and haemorrhage scores were significantly lower compared with Group C, higher scores were found for Group D compared with Group S (p<0.05).
  
  
  CONCLUSIONS
  Pneumoperitoneum caused oxidative injury in rat ovarian tissue. Dexmedetomidine reduced oxidative stress and histological injury related to I/R.
authors.affiliation: Department of Anaesthesiology and Critical Care, Karadeniz Technical University, Faculty of Medicine, Turkey. bahanurcekic@yahoo.com
authors.forename: B
authors.initials: B
authors.lastname: Cekic
authors.forename: A
authors.initials: A
authors.lastname: Besir
authors.forename: E
authors.initials: E
authors.lastname: Yulug
authors.forename: S
authors.initials: S
authors.lastname: Geze
authors.forename: M
authors.initials: M
authors.lastname: Alkanat
mesh_terms: D058647:Adrenergic alpha-2 Receptor Agonists / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D020927:Dexmedetomidine / Q000494:pharmacology / Q000627:therapeutic use*; D004353:Drug Evaluation, Preclinical; D005260:Female; D008315:Malondialdehyde / Q000378:metabolism; D010049:Ovarian Diseases / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*; D010053:Ovary / Q000098:blood supply / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D011028:Pneumoperitoneum, Artificial / Q000009:adverse effects*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000209:etiology / Q000473:pathology / Q000517:prevention & control*
chemical_list: D058647:Adrenergic alpha-2 Receptor Agonists; D008315:Malondialdehyde; D020927:Dexmedetomidine
keywords: Dexmedetomidine; Ischaemia–reperfusion injury; Microscopy; Ovary; Pneumoperitoneum