title: ING4 inhibits the translation of proto-oncogene MYC by interacting with AUF1.
abstract: The ING4 tumor suppressor plays a significant role in various cancer-related cellular processes. AUF1 affects the stability and/or translation of multiple mRNAs via binding to an AU-rich element in the 3'-untranslated regions. In this study, we identify AUF1 as a novel and direct binding partner of ING4. mRNP immunoprecipitation assays indicated that ING4, AUF1 and MYC mRNA present in the same mRNP complex. ING4 suppressed MYC protein expression without altering MYC mRNA levels, and abolished the cell proliferation induced by AUF1 in K562 cells. These results suggest that ING4 may regulate MYC translation by its association with AUF1.
authors.affiliation: Shanghai-MOST Key Laboratory for Disease and Health Genomics, Chinese National Human Genome Center at Shanghai, Shanghai 201203, China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Lu
authors.forename: Chunyang
authors.initials: C
authors.lastname: Pan
authors.forename: Lantian
authors.initials: L
authors.lastname: Zhang
authors.forename: Chenhong
authors.initials: C
authors.lastname: Ding
authors.forename: Fei
authors.initials: F
authors.lastname: Chen
authors.forename: Qinwan
authors.initials: Q
authors.lastname: Wang
authors.forename: Kesheng
authors.initials: K
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
mesh_terms: D018797:Cell Cycle Proteins / Q000737:chemistry / Q000378:metabolism*; D049109:Cell Proliferation; D005786:Gene Expression Regulation; D057809:HEK293 Cells; D006367:HeLa Cells; D000081035:Heterogeneous Nuclear Ribonucleoprotein D0; D034502:Heterogeneous-Nuclear Ribonucleoprotein D / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D047468:Immunoprecipitation; D020014:K562 Cells; D019913:Nuclear Localization Signals; D011485:Protein Binding; D014176:Protein Biosynthesis; D054730:Protein Interaction Domains and Motifs; D020033:Protein Isoforms; D000090063:Proto-Oncogene Mas; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012261:Ribonucleoproteins / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D018797:Cell Cycle Proteins; C000630986:HNRNPD protein, human; D000081035:Heterogeneous Nuclear Ribonucleoprotein D0; D034502:Heterogeneous-Nuclear Ribonucleoprotein D; D018398:Homeodomain Proteins; C483230:ING4 protein, human; C000715470:MAS1 protein, human; C489427:MYC protein, human; D019913:Nuclear Localization Signals; D020033:Protein Isoforms; D000090063:Proto-Oncogene Mas; D016271:Proto-Oncogene Proteins c-myc; D012333:RNA, Messenger; D012261:Ribonucleoproteins; D025521:Tumor Suppressor Proteins; C026452:messenger ribonucleoprotein