title: In vivo phage display--a discovery tool in molecular biomedicine.
abstract: In vivo phage display is a high-throughput method for identifying target ligands specific for different vascular beds. Targeting is possible due to the heterogeneous expression of receptors and other antigens in a particular vascular bed. Such expression is additionally influenced by the physiological or pathological status of the vasculature. In vivo phage display represents a technique that is usable in both, vascular mapping and targeted drug development. In this review, several important methodological aspects of in vivo phage display experiments are discussed. These include choosing an appropriate phage library, an appropriate animal model and the route of phage library administration. In addition, peptides or antibodies identified by in vivo phage display homing to specific types of vascular beds, including the altered vasculature present in several types of diseases are summarized. Still, confirmation in independent experiments and reproduction of identified sequences are needed for enhancing the clinical applicability of in vivo phage display research.
authors.affiliation: Institute of Molecular Biomedicine, Comenius University, Bratislava, Slovakia; Division of Nephrology, RWTH University, Aachen, Germany.
authors.forename: Janka
authors.initials: J
authors.lastname: Bábíčková
authors.forename: Ľubomíra
authors.initials: Ľ
authors.lastname: Tóthová
authors.forename: Peter
authors.initials: P
authors.lastname: Boor
authors.forename: Peter
authors.initials: P
authors.lastname: Celec
mesh_terms: D061505:Cell Surface Display Techniques; D016503:Drug Delivery Systems; D057166:High-Throughput Screening Assays; D006801:Humans; D057054:Molecular Imaging; D062346:Molecular Medicine; D009389:Neovascularization, Pathologic; D019151:Peptide Library
chemical_list: D019151:Peptide Library
keywords: Molecular medicine; Peptide identification; Phage library; Screening; Targeting