title: In vitro transformation of the tremorgenic mycotoxin verruculogen.
abstract: A quantity of 50 mg of crystalline verruculogen was prepared from batch cultures ofAspergillus fumigatus for the use in thein vitro studies with S-9 liver fractions, feces suspensions, and cultures ofEscherichia coli.Incubation of verruculogen with S-9 liver fractions from swine resulted in the transformation of the parent compound into TR-2 toxin and 3 other more polar.TR-2 toxin was also shown to be the main transformation product when incubat-ing verruculogen with fecal suspensions or pure cultures ofE. coli (0149: K88). Incubation times of more than 2 hours led to a complete degradation of verruculogen as well as TR -2 toxin.
authors.affiliation: Institute for Medical Microbiology, Infectious and Epidemic Diseases, Veterinary Faculty, University of Munich, Veterinaerstr 13, W-8000, Munich 22, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Bott
authors.forename: J
authors.initials: J
authors.lastname: Bauer
authors.forename: M
authors.initials: M
authors.lastname: Gareis
authors.forename: C
authors.initials: C
authors.lastname: Enders
authors.forename: B
authors.initials: B
authors.lastname: Kollarczik
authors.forename: B
authors.initials: B
authors.lastname: Gedek