title: Neutralizing activity and protective immunity to ricin toxin conferred by B subunit (RTB)-specific Fab fragments.
abstract: SylH3 and 24B11 are murine monoclonal antibodies directed against different epitopes on ricin toxin's binding (RTB) subunit that have been shown to passively protect mice against ricin challenge. Here we report that Fab fragments of SylH3 and 24B11 neutralize ricin in a cell based assay, and in a mouse challenge model as effectively as their respective full length parental IgGs. These data demonstrate that immunity to ricin can occur independent of Fc-mediated clearance.
authors.affiliation: Division of Infectious Disease, Wadsworth Center, New York State Department of Health, Albany, NY 12208, USA.
authors.forename: Anastasiya
authors.initials: A
authors.lastname: Yermakova
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Mantis
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000494:pharmacology*; D057134:Antibodies, Neutralizing / Q000737:chemistry / Q000494:pharmacology*; D000992:Antitoxins / Q000737:chemistry / Q000494:pharmacology*; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D012276:Ricin / Q000737:chemistry / Q000633:toxicity*; D018675:Toxicity Tests
chemical_list: D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D000992:Antitoxins; D007140:Immunoglobulin Fab Fragments; D012276:Ricin
keywords: Biodefense; Mouse; Neutralizing antibodies; Toxin