title: [Detection and clinicopathologic significance of microsatellite alteration and p53 mutation of neuroendocrine cells in colorectal adenocarcinoma].
abstract: OBJECTIVE
  To study the possible clonal origin of neuroendocrine cells in colorectal adenocarcinoma.
  
  
  METHODS
  Twenty-six microsatellite loci were screened using laser capture microdissection, DNA extraction and whole genome amplification. Microsatellite instability (MSI) and loss of heterozygosity (LOH) in adenocarcinoma cells and neuroendocrine cells amongst 30 cases of colorectal carcinoma with neuroendocrine differentiation were detected using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP)-silver staining. The mutation status of p53 was evaluated by PCR-sequencing. The clonal origin of neuroendocrine cells in colorectal adenocarcinoma was determined.
  
  
  RESULTS
  Amongst the 30 cases studied, the prevalence of MSI was 16.9% while that of LOH was 8.5%. The rate showed no statistically significant difference between adenocarcinoma cells and neuroendocrine cells. In 6 cases, the microsatellite alteration was entirely consistent. In 23 cases, the rate of microsatellite alteration consistency was greater than that of inconsistency. In 1 case, the consistency and inconsistency rates were identical. There was statistically significant difference between consistency and inconsistency of microsatellite alteration. The prevalence of p53 mutation was 16.7% which was the same for both adenocarcinoma cells and neuroendocrine cells.
  
  
  CONCLUSIONS
  Adenocarcinoma cells and neuroendocrine cells in colorectal adenocarcinoma with neuroendocrine differentiation have similar biologic changes. It is likely that they are of identical origin.
authors.affiliation: Pathology and Forensic Medicine Institute, Zhejiang University School of Medicine, Hangzhou 310058, China.
authors.forename: Hai-yong
authors.initials: HY
authors.lastname: Zhang
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Wei
authors.forename: Ling-ling
authors.initials: LL
authors.lastname: Wang
authors.forename: Bu-yi
authors.initials: BY
authors.lastname: Zhang
authors.forename: Zhong-sheng
authors.initials: ZS
authors.lastname: Zhao
authors.forename: Yan-bo
authors.initials: YB
authors.lastname: Lü
authors.forename: Gen-you
authors.initials: GY
authors.lastname: Yao
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D004252:DNA Mutational Analysis; D006801:Humans; D061346:Laser Capture Microdissection; D019656:Loss of Heterozygosity; D053842:Microsatellite Instability; D055099:Neuroendocrine Cells / Q000473:pathology; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: D016159:Tumor Suppressor Protein p53