title: Cholesterol reduction from milk using β-cyclodextrin immobilized on glass.
abstract: β-Cyclodextrin (β-CD) was converted into β-CD-undecenyl ether by chemical modification and subsequently covalently attached to a glass surface. The functionalized glass surface was characterized by static water contact angle and x-ray photoelectron spectroscopy. Both techniques confirmed that an excellent monolayer of β-CD was formed on the glass surface. The β-CD solid surface was used to reduce cholesterol levels in milk. In 4h, 73.6% of the cholesterol was extracted at 25°C with shaking at 170rpm. This is the highest value ever reported for milk using β-CD immobilized on a solid surface. The same surface was repeatedly used for 10 cycles and maintained its efficiency with 72±2% cholesterol reduction observed in all the cycles. X-ray photoelectron spectroscopy analysis completed after 5 and 10 cycles of cholesterol reduction showed that the β-CD on the glass surface was not degraded. The high efficiency and long-term stability of the functional monolayer was attributed to the specific structure of β-CD, which is composed of a relatively low number of functional groups and long spacer chain lengths that provide great flexibility.
authors.affiliation: Department of Bioscience and Biotechnology & Center for Biotechnology Research in UBITA, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Muhammad Nazir
authors.initials: MN
authors.lastname: Tahir
authors.forename: Chanho
authors.initials: C
authors.lastname: Kwon
authors.forename: Daham
authors.initials: D
authors.lastname: Jeong
authors.forename: Eunae
authors.initials: E
authors.lastname: Cho
authors.forename: Seung R
authors.initials: SR
authors.lastname: Paik
authors.forename: Seunho
authors.initials: S
authors.lastname: Jung
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D005511:Food Handling / Q000379:methods*; D005898:Glass; D008892:Milk / Q000737:chemistry*; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D047392:beta-Cyclodextrins; D002784:Cholesterol; C031215:betadex