title: Correlating excipient effects on conformational and storage stability of an IgG1 monoclonal antibody with local dynamics as measured by hydrogen/deuterium-exchange mass spectrometry.
abstract: The effects of sucrose and arginine on the conformational and storage stability of an IgG1 monoclonal antibody (mAb) were monitored by differential scanning calorimetry (DSC) and size-exclusion chromatography (SEC), respectively. Excipient effects on protein physical stability were then compared with their effects on the local flexibility of the mAb in solution at pH 6, 25°C using hydrogen/deuterium-exchange mass spectrometry (H/D-MS). Compared with a 0.1 M NaCl control, sucrose (0.5 M) increased conformational stability (T(m) values), slowed the rate of monomer loss, reduced the formation of insoluble aggregates, and resulted in a global trend of small decreases in local flexibility across most regions of the mAb. In contrast, the addition of arginine (0.5 M) decreased the mAb's conformational stability, increased the rate of loss of monomer with elevated levels of soluble and insoluble aggregates, and led to significant increases in the local flexibility in specific regions of the mAb, most notably within the constant domain 2 of the heavy chain (C(H)2). These results provide new insights into the effect of sucrose and arginine on the local dynamics of IgG1 domains as well as preliminary correlations between local flexibility within specific segments of the C(H)2 domain (notably heavy chain 241-251) and the mAb's overall physical stability.
authors.affiliation: Department of Pharmaceutical Chemistry, Macromolecule and Vaccine Stabilization Center, University of Kansas, Lawrence, Kansas 66047, USA.
authors.forename: Prakash
authors.initials: P
authors.lastname: Manikwar
authors.forename: Ranajoy
authors.initials: R
authors.lastname: Majumdar
authors.forename: John M
authors.initials: JM
authors.lastname: Hickey
authors.forename: Santosh V
authors.initials: SV
authors.lastname: Thakkar
authors.forename: Hardeep S
authors.initials: HS
authors.lastname: Samra
authors.forename: Hasige A
authors.initials: HA
authors.lastname: Sathish
authors.forename: Steven M
authors.initials: SM
authors.lastname: Bishop
authors.forename: C Russell
authors.initials: CR
authors.lastname: Middaugh
authors.forename: David D
authors.initials: DD
authors.lastname: Weis
authors.forename: David B
authors.initials: DB
authors.lastname: Volkin
mesh_terms: D000911:Antibodies, Monoclonal / Q000737:chemistry*; D001120:Arginine / Q000737:chemistry*; D004356:Drug Storage; D005079:Excipients / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry*; D013058:Mass Spectrometry; D056004:Molecular Dynamics Simulation; D011487:Protein Conformation / Q000187:drug effects; D055550:Protein Stability / Q000187:drug effects; D013395:Sucrose / Q000737:chemistry*
chemical_list: D000911:Antibodies, Monoclonal; D005079:Excipients; D007074:Immunoglobulin G; D013395:Sucrose; D001120:Arginine