title: 17β-Estradiol induces changes in cytokine levels in head kidney and blood of juvenile sea bass (Dicentrarchus labrax, L., 1758).
abstract: The cytokine network is involved in the immune system communication. As estrogens influence the cytokine expression in mammals, this study investigated the impact of exogenous estrogenic pollutants on selected cytokines in Dicentrarchus labrax. The gene expression of Interleukin 6, Tumour Necrosis Factor α, Transforming Growth Factor β1 and Interleukin 1β was assessed and accomplished with protein measurements in the blood for the last two. Impacts through 17β-estradiol mainly occurred at the beginning of organ regionalisation, thus falling together with a developmentally induced increase of Interleukin 1β and Tumour Necrosis Factor α gene expression in 102 dph fish. 17β-estradiol depressed this modification after 35 days of exposure and the cytokine gene expression tended to be generally down-regulated independently of the 17β-estradiol concentrations after 56 days of exposure. This impact was confirmed at the protein level, showing that 17β-estradiol affects the fine control of the cytokine network in sea bass.
authors.affiliation: Laboratoire d'Écotoxicologie - Milieux Aquatiques (LEMA), SFR SCALE 4116, Université du Havre, 25 Rue Philippe Lebon, 76058 Le Havre Cedex, France. frauke.seemann@univ-lehavre.fr
authors.forename: Frauke
authors.initials: F
authors.lastname: Seemann
authors.forename: Thomas
authors.initials: T
authors.lastname: Knigge
authors.forename: Béatrice
authors.initials: B
authors.lastname: Rocher
authors.forename: Christophe
authors.initials: C
authors.lastname: Minier
authors.forename: Tiphaine
authors.initials: T
authors.lastname: Monsinjon
mesh_terms: D000818:Animals; D001492:Bass / Q000254:growth & development / Q000378:metabolism*; D016207:Cytokines / Q000097:blood / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004781:Environmental Exposure; D004784:Environmental Monitoring; D004958:Estradiol / Q000378:metabolism*; D004967:Estrogens / Q000378:metabolism; D029941:Fish Proteins / Q000097:blood / Q000378:metabolism; D005786:Gene Expression Regulation; D060226:Head Kidney / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D007107:Immune System / Q000254:growth & development; D060888:Real-Time Polymerase Chain Reaction; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D016207:Cytokines; D004967:Estrogens; D029941:Fish Proteins; D014874:Water Pollutants, Chemical; D004958:Estradiol