title: Lab-on-a-Chip for anticancer drug screening using quantum dots probe based apoptosis assay.
abstract: Quantitative data acquisition of the drug induced cell apoptosis is one of the most important aspects of anticancer drug assessment. Traditional methods using photo-bleachable organic dyes to detect the apoptotic cells, may lead to some difficulties in long time observation of the cells. Moreover, the culture plates inevitably require large number of cells resulting in inefficiency in analysis of fewer cell samples such as a patient's own cells. To address these problems, we developed a microfluidic platform to assess the adherent cell cytotoxicity for anticancer drug screening using Annexin V conjugated quantum dots and Calcein AM as dual apoptotic probes. The results showed that camptothecin possessed more potent cytotoxic effect on these adherent tumor cells. Interestingly, the MCF-7 exhibited a certain resistance to these two anti-tumor drugs. For the first time, we achieved the evaluation of the impacts of model anti-cancer drugs on different adherent tumor cell lines using bio-functional quantum dots probe based cytotoxicity assays in a microfluidic device.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhao
authors.forename: Jun-Tao
authors.initials: JT
authors.lastname: Caot
authors.forename: Zeng-Qiang
authors.initials: ZQ
authors.lastname: Wu
authors.forename: Jian-Xin
authors.initials: JX
authors.lastname: Li
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Zhu
mesh_terms: D000970:Antineoplastic Agents; D017209:Apoptosis / Q000187:drug effects; D001681:Biological Assay / Q000379:methods*; D002166:Camptothecin / Q000494:pharmacology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003198:Computer Simulation; D004354:Drug Screening Assays, Antitumor / Q000379:methods*; D005047:Etoposide / Q000494:pharmacology; D006801:Humans; D056656:Lab-On-A-Chip Devices; D045663:Quantum Dots
chemical_list: D000970:Antineoplastic Agents; D005047:Etoposide; D002166:Camptothecin