title: The phenyl-thiophenyl propenone RK-I-123 reduces the levels of reactive oxygen species and suppresses the activation of NF-κB and AP-1 and IL-8 expression in Helicobacter pylori-infected gastric epithelial AGS cells.
abstract: OBJECTIVE
  To investigate whether the phenyl-thiophenyl propenone RK-I-123 suppresses interleukin-8 (IL-8) expression and activation of mitogen-activated protein kinases (MAPKs) and transcription factors (nuclear factor-κB [NF-κB] and activator protein-1 [AP-1]) by reducing reactive oxygen species (ROS) levels in Helicobacter pylori-infected gastric epithelial cells.
  
  
  METHODS
  Helicobacter pylori in Korean isolates, human gastric epithelial AGS cells.
  
  
  METHODS
  AGS cells pretreated with or without RK-I-123 were cultured in the presence of H. pylori at a bacterium/cell ratio of 300:1.
  
  
  METHODS
  Reactive oxygen species and IL-8 levels were determined by dichlorofluorescein fluorescence and enzyme-linked immunosorbent assay. The IL-8 mRNA expression was analyzed by the real-time reverse transcription-polymerase chain reaction (RT-PCR). The MAPK and IκBα levels were determined by western blotting. The activation of NF-κB and AP-1 was determined by the electrophoretic mobility shift assay.
  
  
  RESULTS
  Helicobacter pylori induced an increase in ROS and IL-8 expression and activation of MAPKs and transcription factors (NF-κB and AP-1) together with the degradation of IκBα in AGS cells, all of which were inhibited by RK-I-123.
  
  
  CONCLUSIONS
  The RK-I-123 suppressed the H. pylori-induced IL-8 expression and activation of MAPKs, NF-κB, and AP-1 by reducing ROS levels in AGS cells. The RK-I-123 may be a potential candidate for the treatment of H. pylori-induced gastric inflammation.
authors.affiliation: Department of Food and Nutrition, Brain Korea 21 Project, College of Human Ecology, Yonsei University, Seoul 120-749, Korea.
authors.forename: Sung Hee
authors.initials: SH
authors.lastname: Jang
authors.forename: Soonok
authors.initials: S
authors.lastname: Cho
authors.forename: Eung-Seok
authors.initials: ES
authors.lastname: Lee
authors.forename: Jung Mogg
authors.initials: JM
authors.lastname: Kim
authors.forename: Hyeyoung
authors.initials: H
authors.lastname: Kim
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002478:Cells, Cultured; D047188:Chalcones / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects* / Q000276:immunology / Q000382:microbiology; D005753:Gastric Mucosa / Q000166:cytology / Q000382:microbiology; D016481:Helicobacter Infections / Q000276:immunology* / Q000382:microbiology; D016480:Helicobacter pylori; D006801:Humans; D016209:Interleukin-8 / Q000276:immunology*; D016328:NF-kappa B / Q000276:immunology; D017382:Reactive Oxygen Species / Q000276:immunology; D013876:Thiophenes / Q000494:pharmacology*; D018808:Transcription Factor AP-1 / Q000276:immunology
chemical_list: C584561:1-phenyl-3-(thiophen-2-yl)prop-2-en-1-one; D000893:Anti-Inflammatory Agents; D047188:Chalcones; D016209:Interleukin-8; D016328:NF-kappa B; D017382:Reactive Oxygen Species; D013876:Thiophenes; D018808:Transcription Factor AP-1