title: Comparative proteomic profiling in compatible and incompatible interactions between hop roots and Verticillium albo-atrum.
abstract: Verticillium wilt, caused by the soil borne fungal pathogen Verticillium albo-atrum, is a serious threat to hop (Humulus lupulus L.) production in several hop-growing regions. A proteomic approach was applied to analyse the response of root tissue in compatible and incompatible interactions between hop and V. albo-atrum at 10, 20 and 30 days after inoculation, using two-dimensional difference gel electrophoresis (2D-DIGE) coupled with de novo sequencing of derivatized peptides. Approximately 1200 reproducible spots were detected on the gels, of which 102 were identified. In the compatible interaction, 252 spots showed infection-specific changes in spot abundance and an accumulation of defence-related proteins, such as chitinase, β-glucanase, thaumatin-like protein, peroxidase and germin-like protein, was observed. However, no significant infection-specific changes were detected in the incompatible interaction. The results indicate that resistance in this pathosystem may be conferred by constitutive rather than induced defence mechanisms. The identification and high abundance of two mannose/glucose-specific lectin isoforms present only in the roots of the resistant cultivar suggests function of lectins in hop resistance against V. albo-atrum.
authors.affiliation: Biotechnical Faculty, Department of Agronomy, University of Ljubljana, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia.
authors.forename: Stanislav
authors.initials: S
authors.lastname: Mandelc
authors.forename: Isaak
authors.initials: I
authors.lastname: Timperman
authors.forename: Sebastjan
authors.initials: S
authors.lastname: Radišek
authors.forename: Bart
authors.initials: B
authors.lastname: Devreese
authors.forename: Bart
authors.initials: B
authors.lastname: Samyn
authors.forename: Branka
authors.initials: B
authors.lastname: Javornik
mesh_terms: D000595:Amino Acid Sequence; D054884:Host-Pathogen Interactions / Q000502:physiology*; D027582:Humulus / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology*; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000382:microbiology; D037121:Plant Lectins / Q000378:metabolism; D010940:Plant Proteins / Q000032:analysis / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism / Q000382:microbiology*; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D057825:Two-Dimensional Difference Gel Electrophoresis; D020172:Verticillium / Q000378:metabolism* / Q000472:pathogenicity*
chemical_list: D037121:Plant Lectins; D010940:Plant Proteins