title: Species-specific impact of the autophagy machinery on Chikungunya virus infection.
abstract: Chikungunya virus (CHIKV) is a recently re-emerged arbovirus that triggers autophagy. Here, we show that CHIKV interacts with components of the autophagy machinery during its replication cycle, inducing a cytoprotective effect. The autophagy receptor p62 protects cells from death by binding ubiquitinated capsid and targeting it to autophagolysosomes. By contrast, the human autophagy receptor NDP52--but not its mouse orthologue--interacts with the non-structural protein nsP2, thereby promoting viral replication. These results highlight the distinct roles of p62 and NDP52 in viral infection, and identify NDP52 as a cellular factor that accounts for CHIKV species specificity.
authors.affiliation: Institut Pasteur, Biology of Infection Unit, Paris, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Judith
authors.forename: Serge
authors.initials: S
authors.lastname: Mostowy
authors.forename: Mehdi
authors.initials: M
authors.lastname: Bourai
authors.forename: Nicolas
authors.initials: N
authors.lastname: Gangneux
authors.forename: Mickaël
authors.initials: M
authors.lastname: Lelek
authors.forename: Marianne
authors.initials: M
authors.lastname: Lucas-Hourani
authors.forename: Nadège
authors.initials: N
authors.lastname: Cayet
authors.forename: Yves
authors.initials: Y
authors.lastname: Jacob
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Prévost
authors.forename: Philippe
authors.initials: P
authors.lastname: Pierre
authors.forename: Frédéric
authors.initials: F
authors.lastname: Tangy
authors.forename: Christophe
authors.initials: C
authors.lastname: Zimmer
authors.forename: Pierre-Olivier
authors.initials: PO
authors.lastname: Vidalain
authors.forename: Thérèse
authors.initials: T
authors.lastname: Couderc
authors.forename: Marc
authors.initials: M
authors.lastname: Lecuit
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D018354:Alphavirus Infections / Q000821:virology*; D000818:Animals; D001343:Autophagy; D002213:Capsid / Q000378:metabolism; D065632:Chikungunya Fever; D002646:Chikungunya virus / Q000502:physiology*; D006367:HeLa Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D007113:Immunity, Innate; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D010588:Phagosomes / Q000378:metabolism / Q000821:virology; D011485:Protein Binding; D021381:Protein Transport; D000071456:Sequestosome-1 Protein; D020123:Sirolimus / Q000494:pharmacology; D013045:Species Specificity; D017361:Viral Nonstructural Proteins / Q000378:metabolism; D014779:Virus Replication
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C094297:CALCOCO2 protein, human; C477012:MAP1LC3A protein, human; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; C099718:SQSTM1 protein, human; D000071456:Sequestosome-1 Protein; D017361:Viral Nonstructural Proteins; D020123:Sirolimus