title: Exploration of Brazilian biodiversity and selection of a new oleaginous yeast strain cultivated in raw glycerol.
abstract: The objective of this study was to use glycerol generated from the synthesis of biodiesel to study the oleaginous potential of wild yeasts. An initial selection was performed via a rapid and qualitative technique by staining with Sudan Black B. Initially 129 yeasts were present, from which 5 were selected and cultivated in liquid medium containing pure or raw glycerol. The yeast LEB-M3, isolated from the Pantanal, presented lipid content of 20.46% and 56.58% for cultivation in pure and raw glycerol, respectively. This strain was genotypically identified as Candida sp. The fatty acid profile showed predominance of oleic acid (C18:1), 57.35% for cultivation in pure glycerol, and in raw glycerol linoleic acid (C18:2) was predominant (46.0%). It was possible to select a yeast with high lipid concentrations 9.14 g/L and fatty acid profile similar to vegetable oils commonly used in the synthesis of biodiesel.
authors.affiliation: Laboratory of Bioprocess Engineering, Faculty of Food Engineering - UNICAMP, Campinas, Brazil. susanduarte@hotmail.com
authors.forename: Susan Hartwig
authors.initials: SH
authors.lastname: Duarte
authors.forename: Cristiane Conte Paim
authors.initials: CC
authors.lastname: de Andrade
authors.forename: Gislaine
authors.initials: G
authors.lastname: Ghiselli
authors.forename: Francisco
authors.initials: F
authors.lastname: Maugeri
mesh_terms: D044822:Biodiversity; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D001938:Brazil; D002175:Candida / Q000187:drug effects* / Q000254:growth & development*; D005227:Fatty Acids / Q000378:metabolism; D005990:Glycerol / Q000494:pharmacology*; D008055:Lipids / Q000737:chemistry*
chemical_list: D005227:Fatty Acids; D008055:Lipids; D005990:Glycerol