title: On-line solid phase extraction using organic-inorganic hybrid monolithic columns for the determination of trace β-lactam antibiotics in milk and water samples.
abstract: A rapid and simple method with on-line solid phase extraction (SPE) has been developed for the simultaneous determination of beta-lactam antibiotics (BLAs) (amoxicillin, cephradine, and cefazolin sodium) in aquatic environment and milk. The epoxy-based organic-inorganic hybrid monolithic column was used as SPE sorbent to simultaneously monitor three analytes. The morphology of monolithic column and pressure drop across the columns were characterized. Excellent permeability and high selectivity were obtained. The linear range of the standard curve was from 2.0 to 500 ng/mL (r(2)≥0.999). Precision for inter- and intra-day assay showed acceptable results for quantitative assay with relative standard deviation (RSD) less than 11%. The accuracy and recovery were found to be within the range of 93-103% and 83-105%. The results indicated that the prepared monolithic column could provide excellent reproducibility and implied that the prepared monolith was feasible to be used as an on-line SPE sorbent material.
authors.affiliation: College of Pharmacy, Hebei University, Hebei Province Key Laboratory of Pharmaceutical Quality Control, Baoding 071002, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Haiyan
authors.initials: H
authors.lastname: Liu
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhang
authors.forename: Huan
authors.initials: H
authors.lastname: Lei
authors.forename: Ligai
authors.initials: L
authors.lastname: Bai
authors.forename: Gengliang
authors.initials: G
authors.lastname: Yang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D001559:Benzhydryl Compounds / Q000737:chemistry; D002681:China; D002851:Chromatography, High Pressure Liquid; D004784:Environmental Monitoring; D004853:Epoxy Resins / Q000737:chemistry; D008892:Milk / Q000737:chemistry*; D009862:Online Systems; D010636:Phenols / Q000737:chemistry; D045483:Rivers / Q000737:chemistry; D012821:Silanes / Q000737:chemistry; D052616:Solid Phase Extraction; D014874:Water Pollutants, Chemical / Q000032:analysis*; D047090:beta-Lactams / Q000032:analysis*; D000098826:Bisphenol A Compounds
chemical_list: D000900:Anti-Bacterial Agents; D001559:Benzhydryl Compounds; D004853:Epoxy Resins; D010636:Phenols; D012821:Silanes; D014874:Water Pollutants, Chemical; D047090:beta-Lactams; C040733:tetraethoxysilane; C006780:bisphenol A; D000098826:Bisphenol A Compounds