title: Protective effect of magnesium lithospermate B against dextran sodiumsulfate induced ulcerative colitis in mice.
abstract: Anti-platelet drugs have been used to treat inflammatory bowel disease. In this study, we observed the therapeutic effects of magnesium lithospermate B, a main component of salvianolate, on colitis induced by dextran sodiumsulfate (DSS). Colitis was induced by 5% DSS oral administration in BALB/C male mice. Magnesium lithospermate B (60-240mg/kg) was given by subcutaneous injection for 2 weeks. Then, mice were sacrificed; serum and colon tissues were collected for biomarker assay, histological examination, immunohistochemical study and real-time quantitative polymerase chain reaction. DSS induced gross bleeding, inflammation, crypt damage and mucosal damage in colon. Treatment with magnesium lithospermate B could reduce colon inflammation induced by DSS. Magnesium lithospermate B could reverse the high CD40/CD40L expression and hypercoagulable state induced by DSS in colon. This study showed that magnesium lithospermate B could be used to treat colitis. The protective effects of magnesium lithospermate B may be due to its effects on CD40/CD40L expression and blood clotting status.
authors.affiliation: Department of Gastroenterology, Huashan Hospital, Fudan University, China.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Jiang
authors.forename: Yibin
authors.initials: Y
authors.lastname: Jiang
authors.forename: Dayu
authors.initials: D
authors.lastname: Sun
authors.forename: Lan
authors.initials: L
authors.lastname: Rong
mesh_terms: D000818:Animals; D019013:CD40 Antigens / Q000235:genetics / Q000378:metabolism; D023201:CD40 Ligand / Q000235:genetics / Q000378:metabolism; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D016264:Dextran Sulfate; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D015850:Interleukin-6 / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D020011:Protective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D058258:Serpin E2 / Q000097:blood; D010959:Tissue Plasminogen Activator / Q000097:blood
chemical_list: D019013:CD40 Antigens; D004365:Drugs, Chinese Herbal; D015850:Interleukin-6; D020011:Protective Agents; D058258:Serpin E2; C511869:Serpine2 protein, mouse; C061091:lithospermate B; D023201:CD40 Ligand; D016264:Dextran Sulfate; D010959:Tissue Plasminogen Activator