title: Glycyrrhizic acid nanoparticles inhibit LPS-induced inflammatory mediators in 264.7 mouse macrophages compared with unprocessed glycyrrhizic acid.
abstract: Glycyrrhizic acid (GA), the main component of radix glycyrrhizae, has a variety of pharmacological activities. In the present study, suspensions of GA nanoparticles with the average particle size about 200 nm were prepared by a supercritical antisolvent (SAS) process. Comparative studies were undertaken using lipopolysaccardide (LPS)-stimulated mouse macrophages RAW 264.7 as in vitro inflammatory model. Several important inflammation mediators such as NO, PGE2, TNF-α and IL-6 were examined. These markers were highly stimulated by LPS and were inhibited both by nano-GA and unprocessed GA in a dose-dependent manner, especially PGE2 and TNF-α. However nano-GA and unprocessed GA inhibited NO only at a high concentration. In general, we found that GA nanoparticle suspensions exhibited much better anti-inflammatory activities compared to unprocessed GA.
authors.affiliation: Key Laboratory of Forest Plant Ecology, Northeast Forestry University, Harbin, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Meng
authors.initials: M
authors.lastname: Luo
authors.forename: Yujie
authors.initials: Y
authors.lastname: Fu
authors.forename: Song
authors.initials: S
authors.lastname: Wang
authors.forename: Thomas
authors.initials: T
authors.lastname: Efferth
authors.forename: Yuangang
authors.initials: Y
authors.lastname: Zu
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry*; D019695:Glycyrrhizic Acid / Q000737:chemistry* / Q000494:pharmacology*; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D009569:Nitric Oxide; D013499:Surface Properties; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D004337:Drug Carriers; D015850:Interleukin-6; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D019695:Glycyrrhizic Acid
keywords: glycyrrhizic acid; inflammatory cytokines; mouse macrophages RAW 264.7; nanoparticle