title: Accelerating repaired basement membrane after bevacizumab treatment on alkali-burned mouse cornea.
abstract: To understand the corneal regeneration induced by bevacizumab, we investigated the structure changes of stroma and basement membrane regeneration. A Stick soaked in 0.5 N NaOH onto the mouse cornea and 2.5 mg/ml of bevacizumab was delivered into an alkali-burned cornea (2 μl) by subconjunctival injections at 1 hour and 4 days after injury. At 7 days after injury, basement membrane regeneration was observed by transmission electron microscope. Uneven and thin epithelial basement membrane, light density of hemidesmosomes, and edematous collagen fibril bundles are shown in the alkali-burned cornea. Injured epithelial basement membrane and hemidesmosomes and edematous collagen fibril bundles resulting from alkali-burned mouse cornea was repaired by bevacizumab treatment. This study demonstrates that bevacizumab can play an important role in wound healing in the cornea by accelerating the reestablishment of basement membrane integrity that leads to barriers for scar formation.
authors.affiliation: Department of Optometry, Eulji University, Seongnam 461-713, Korea. kjl@eulji.ac.kr
authors.forename: Koon-Ja
authors.initials: KJ
authors.lastname: Lee
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Lee
authors.forename: Sung Ho
authors.initials: SH
authors.lastname: Lee
authors.forename: Tae Hoon
authors.initials: TH
authors.lastname: Choi
mesh_terms: D000468:Alkalies / Q000633:toxicity*; D020533:Angiogenesis Inhibitors / Q000627:therapeutic use*; D000818:Animals; D061067:Antibodies, Monoclonal, Humanized / Q000627:therapeutic use*; D000068258:Bevacizumab; D002057:Burns, Chemical; D024141:Collagen Type IV / Q000378:metabolism; D019573:Epithelium, Corneal / Q000187:drug effects / Q000473:pathology; D005126:Eye Burns / Q000139:chemically induced / Q000188:drug therapy*; D022002:Hemidesmosomes / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D046529:Microscopy, Electron, Transmission; D012038:Regeneration
chemical_list: D000468:Alkalies; D020533:Angiogenesis Inhibitors; D061067:Antibodies, Monoclonal, Humanized; D024141:Collagen Type IV; D000068258:Bevacizumab