title: Efficient optimization of ultra-high- performance supercritical fluid chromatographic separation of Rosa sericea by response surface methodology.
abstract: An approach for rapid optimization of ultra-high-performance supercritical fluid chromatographic (UHPSFC) gradient by response surface methodology was developed for fast separation of complex crude extracts of the leaves of Rosa sericea. The optimization was performed with Box-Behnken designs and the multicriteria response variables were described using Derringer's desirability. Based on factorial design experiments, five factors were selected for Box-Behnken designs to optimize the UHPSFC conditions, which led to 46 experiments being performed within 8 h. An evaporative light-scattering detector (ELSD) was used, and quantitative analysis of main components in R. sericea samples was employed to evaluate the statistical significance of the parameters on UHPSFC-ELSD analytes response. The results indicated that the optimized UHPSFC-ELSD method is very sensitive with LODs and LOQs below 1.19 and 4.55 μg/mL, respectively. The overall intra- and interday variations were less than 3.91 and 6.41%, respectively. The recovery of the method ranged from 95.66 to 104.22%, with RSD < 5.91%. This newly developed UHPSFC-ELSD method was demonstrated to be fast and sensitive in analyzing complex herbal extracts of Traditional Chinese Medicines.
authors.affiliation: Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, P R China.
authors.forename: Jin-Rong
authors.initials: JR
authors.lastname: Li
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.forename: Bing
authors.initials: B
authors.lastname: Xia
authors.forename: Li-Sheng
authors.initials: LS
authors.lastname: Ding
authors.forename: Hong-Xi
authors.initials: HX
authors.lastname: Xu
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D057230:Limit of Detection; D018515:Plant Leaves / Q000737:chemistry; D015203:Reproducibility of Results; D027826:Rosa / Q000737:chemistry*
keywords: Box-Behnken design; Evaporative light scattering detector; Response surface methodology; Rosa sericea; Ultra-high performance supercritical fluid chromatography