title: Potentiated therapeutic angiogenesis by primed human mesenchymal stem cells in a mouse model of hindlimb ischemia.
abstract: BACKGROUND
  Human bone marrow-derived mesenchymal stem cells (hMSCs) are advantageous for cell-based therapy to treat ischemic diseases owing to their capacity to secrete various paracrine factors with potent angiogenic activity.
  
  
  METHODS
  In this study, we describe a method to increase secreted levels of VEGF and HGF from hMSCs without genetic modification.
  
  
  RESULTS
  We demonstrated that transplantation of primed hMSCs into ischemic limbs led to significantly greater improvements in tissue perfusion and limb salvage by increasing capillary formation compared with nonprimed hMSCs. The primed hMSCs also exhibited greater survival in vivo and secreted human VEGF and HGF in the ischemic tissue, supporting enhanced angiogenesis and cell survival.
  
  
  CONCLUSIONS
  These findings indicate that priming hMSCs via methods described in this study enhances secretion of critical proangiogenic factors resulting in an enhanced therapeutic effect of cells for the treatment of ischemic diseases.
authors.affiliation: National Research Laboratory for Cardiovascular Stem Cells & IRICT, Seoul National University Hospital, Seoul, Republic of Korea.
authors.forename: Eun Ju
authors.initials: EJ
authors.lastname: Lee
authors.forename: Hwan-Woo
authors.initials: HW
authors.lastname: Park
authors.forename: Hyo-Jin
authors.initials: HJ
authors.lastname: Jeon
authors.forename: Hyo-Soo
authors.initials: HS
authors.lastname: Kim
authors.forename: Mi-Sook
authors.initials: MS
authors.lastname: Chang
mesh_terms: D000328:Adult; D000818:Animals; D004195:Disease Models, Animal; D017228:Hepatocyte Growth Factor / Q000378:metabolism; D006614:Hindlimb / Q000098:blood supply* / Q000473:pathology / Q000503:physiopathology*; D006801:Humans; D007511:Ischemia / Q000473:pathology / Q000503:physiopathology* / Q000628:therapy*; D023821:Limb Salvage; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D018919:Neovascularization, Physiologic; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D042461:Vascular Endothelial Growth Factor A; D017228:Hepatocyte Growth Factor