title: cDNA-AFLP-based transcript profiling for genome-wide expression analysis of jasmonate-treated plants and plant cultures.
abstract: cDNA-AFLP is a commonly used, robust, and reproducible tool for genome-wide expression analysis in any species, without requirement of prior sequence knowledge. Quantitative expression data are generated by gel-based visualization of cDNA-AFLP fingerprints obtained by selective PCR amplification of subsets of restriction fragments from a double-stranded cDNA template. Differences in gene expression levels across the samples are reflected in different band intensities on the high-resolution polyacrylamide gels. The differentially expressed genes can be identified by direct sequencing of re-amplified cDNA-AFLP tags purified from the gels. The cDNA-AFLP technique is especially useful for profiling of transcriptional responses of jasmonate-treated plants or plant (tissue) cultures and the discovery of jasmonate-responsive genes.
authors.affiliation: Department of Plant Systems Biology, VIB, Gent, Belgium.
authors.forename: Janine
authors.initials: J
authors.lastname: Colling
authors.forename: Jacob
authors.initials: J
authors.lastname: Pollier
authors.forename: Nokwanda P
authors.initials: NP
authors.lastname: Makunga
authors.forename: Alain
authors.initials: A
authors.lastname: Goossens
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D046508:Culture Techniques; D003517:Cyclopentanes / Q000494:pharmacology*; D018076:DNA, Complementary / Q000235:genetics*; D015870:Gene Expression; D020869:Gene Expression Profiling; D018745:Genome, Plant; D054883:Oxylipins / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction
chemical_list: D003517:Cyclopentanes; D018076:DNA, Complementary; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; C011006:jasmonic acid