title: Improving the understanding of oligonucleotide-nanoparticle conjugates using DNA-binding fluorophores.
abstract: A novel fluorescent-based method for characterisation of oligonucleotide-nanoparticle conjugates (ONPCs) is reported. We exploit the ability of the double-stranded DNA-binding dye, SYBR Green I, to develop a powerful analytical tool to investigate the melting properties and hybridisation behavior of ONPCs as well as their corresponding DNA-mediated assemblies.
authors.affiliation: Centre for Molecular Nanometrology, Department of Pure and Applied Chemistry, University of Strathclyde, 295 Cathedral Street, Glasgow G1 1YL, UK. luca.guerrini@ctqc.org
authors.forename: Luca
authors.initials: L
authors.lastname: Guerrini
authors.forename: Lee
authors.initials: L
authors.lastname: Barrett
authors.forename: Jennifer A
authors.initials: JA
authors.lastname: Dougan
authors.forename: Karen
authors.initials: K
authors.lastname: Faulds
authors.forename: Duncan
authors.initials: D
authors.lastname: Graham
mesh_terms: D052160:Benzothiazoles; D004247:DNA / Q000737:chemistry*; D003959:Diamines; D005095:Exoribonucleases / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D009693:Nucleic Acid Hybridization / Q000379:methods; D009930:Organic Chemicals / Q000737:chemistry*; D011804:Quinolines
chemical_list: D052160:Benzothiazoles; D003959:Diamines; D005456:Fluorescent Dyes; D009930:Organic Chemicals; D011804:Quinolines; C098022:SYBR Green I; D004247:DNA; D005095:Exoribonucleases; C065486:oligonucleotidase