title: Putative drug and vaccine target protein identification using comparative genomic analysis of KEGG annotated metabolic pathways of Mycoplasma hyopneumoniae.
abstract: In the present study, a computational comparative and subtractive genomic/proteomic analysis aimed at the identification of putative therapeutic target and vaccine candidate proteins from Kyoto Encyclopedia of Genes and Genomes (KEGG) annotated metabolic pathways of Mycoplasma hyopneumoniae was performed for drug design and vaccine production pipelines against M.hyopneumoniae. The employed comparative genomic and metabolic pathway analysis with a predefined computational systemic workflow extracted a total of 41 annotated metabolic pathways from KEGG among which five were unique to M. hyopneumoniae. A total of 234 proteins were identified to be involved in these metabolic pathways. Although 125 non homologous and predicted essential proteins were found from the total that could serve as potential drug targets and vaccine candidates, additional prioritizing parameters characterize 21 proteins as vaccine candidate while druggability of each of the identified proteins evaluated by the DrugBank database prioritized 42 proteins suitable for drug targets.
authors.affiliation: Laboratory of Veterinary Pharmacokinetics & Pharmacodynamics, College of Veterinary Medicine, Kyungpook National University, Daegu 702-701, Republic of Korea.
authors.forename: Dereje
authors.initials: D
authors.lastname: Damte
authors.forename: Joo-Won
authors.initials: JW
authors.lastname: Suh
authors.forename: Seung-Jin
authors.initials: SJ
authors.lastname: Lee
authors.forename: Sileshi Belew
authors.initials: SB
authors.lastname: Yohannes
authors.forename: Md Akil
authors.initials: MA
authors.lastname: Hossain
authors.forename: Seung-Chun
authors.initials: SC
authors.lastname: Park
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D019295:Computational Biology; D062126:Databases, Chemical; D015195:Drug Design; D016680:Genome, Bacterial; D006801:Humans; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D058977:Molecular Sequence Annotation; D045705:Mycoplasma hyopneumoniae / Q000187:drug effects / Q000378:metabolism* / Q000472:pathogenicity; D014614:Vaccines, Synthetic / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D014614:Vaccines, Synthetic