title: Dual-responsive surfaces modified with phenylboronic acid-containing polymer brush to reversibly capture and release cancer cells.
abstract: Artificial stimuli-responsive surfaces that can mimic the dynamic function of living systems have attracted much attention. However, there exist few artificial systems capable of responding to dual- or multistimulation as the natural system does. Herein, we synthesize a pH and glucose dual-responsive surface by grafting poly(acrylamidophenylboronic acid) (polyAAPBA) brush from aligned silicon nanowire (SiNW) array. The as-prepared surface can reversibly capture and release targeted cancer cells by precisely controlling pH and glucose concentration, exhibiting dual-responsive AND logic. In the presence of 70 mM glucose, the surface is pH responsive, which can vary from a cell-adhesive state to a cell-repulsive state by changing the pH from 6.8 to 7.8. While keeping the pH at 7.8, the surface becomes glucose responsive--capturing cells in the absence of glucose and releasing cells by adding 70 mM glucose. Through simultaneously changing the pH and glucose concentration from pH 6.8/0 mM glucose to pH 7.8/70 mM glucose, the surface is dual responsive with the capability to switch between cell capture and release for at least 5 cycles. The cell capture and release process on this dual-responsive surface is noninvasive with cell viability higher than 95%. Moreover, topographical interaction between the aligned SiNW array and cell protrusions greatly amplifies the responsiveness and accelerates the response rate of the dual-responsive surface between cell capture and release. The responsive mechanism of the dual-responsive surface is systematically studied using a quartz crystal microbalance, which shows that the competitive binding between polyAAPBA/sialic acid and polyAAPBA/glucose contributes to the dual response. Such dual-responsive surface can significantly impact biomedical and biological applications including cell-based diagnostics, in vivo drug delivery, etc.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Liu
authors.forename: Yingying
authors.initials: Y
authors.lastname: Li
authors.forename: Kang
authors.initials: K
authors.lastname: Sun
authors.forename: Junbing
authors.initials: J
authors.lastname: Fan
authors.forename: Pengchao
authors.initials: P
authors.lastname: Zhang
authors.forename: Jingxin
authors.initials: J
authors.lastname: Meng
authors.forename: Shutao
authors.initials: S
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Jiang
mesh_terms: D000178:Acrylamides / Q000737:chemistry*; D001897:Boronic Acids / Q000737:chemistry*; D005947:Glucose / Q000138:chemical synthesis / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D061986:MCF-7 Cells; D015394:Molecular Structure; D053770:Nanowires / Q000737:chemistry; D010316:Particle Size; D012825:Silicon / Q000737:chemistry; D013499:Surface Properties
chemical_list: D000178:Acrylamides; D001897:Boronic Acids; C585173:poly(acrylamidophenylboronicacid); D005947:Glucose; C010686:benzeneboronic acid; D012825:Silicon