title: Evaluation of intracellular lipid bodies in Chlamydomonas reinhardtii strains by flow cytometry.
abstract: A comparative study of Chlamydomonas reinhardtii wild type CC124 and a cell wall-less mutant sta6-1 is described using FACS in conjunction with two different lipophilic fluorescent dyes, Nile Red and BODIPY 505/515. The results indicate that BODIPY 505/515 is more effective for the vital staining of intracellular lipid bodies and single cell sorting than Nile Red. While BODIPY 505/515 stained cells continued to grow after single cell sorting using FACS, Nile Red stained cells failed to recover from sorting. In addition, a comprehensive study was performed to establish a quantitative baseline for future studies for either lipid accumulation and/or microalgal growth by measuring various parameters such as cell count, size, fatty acid contents/composition, and optical/confocal images of the wild type and mutant.
authors.affiliation: Department of Chemical and Biomolecular Engineering, KAIST, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Natarajan
authors.initials: N
authors.lastname: Velmurugan
authors.forename: Minji
authors.initials: M
authors.lastname: Sung
authors.forename: Sung Sun
authors.initials: SS
authors.lastname: Yim
authors.forename: Min S
authors.initials: MS
authors.lastname: Park
authors.forename: Ji Won
authors.initials: JW
authors.lastname: Yang
authors.forename: Ki Jun
authors.initials: KJ
authors.lastname: Jeong
mesh_terms: D001896:Boron Compounds / Q000378:metabolism; D048429:Cell Size; D016825:Chlamydomonas reinhardtii / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism*; D002849:Chromatography, Gas; D004952:Esters / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005410:Flame Ionization; D005434:Flow Cytometry / Q000379:methods*; D005453:Fluorescence; D042541:Intracellular Space / Q000378:metabolism*; D008055:Lipids / Q000737:chemistry*; D009154:Mutation / Q000235:genetics; D010078:Oxazines / Q000378:metabolism; D013194:Staining and Labeling; D013213:Starch / Q000378:metabolism
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D004952:Esters; D005227:Fatty Acids; D008055:Lipids; D010078:Oxazines; D013213:Starch; C044808:nile red