title: Breaking the conservation of guanine residues in the catalytic loop of 10-23 DNAzyme by position-specific nucleobase modifications for rate enhancement.
abstract: 10-23 DNAzyme has been used as a very good model for exploring the potential of functional nucleic acids. Its 15-mer catalytic core has been demonstrated to be the optimized in vitro selection result. However, with the introduction of protein-like functional groups through 2'-deoxyguanosine analogues, its two highly conserved guanine residues (G2 and G14) can be modified for more efficient 10-23 DNAzyme analogues. This result implies that chemical modifications of functional nucleic acids with well-designed nucleoside analogues of each canonical residue could be used as the first step in the efforts toward more powerful functions of nucleic acids.
authors.affiliation: School of Pharmacological Sciences, Guangxi Medical University, Nanning, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhiwen
authors.initials: Z
authors.lastname: Li
authors.forename: Gaofeng
authors.initials: G
authors.lastname: Liu
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Di
authors.initials: D
authors.lastname: Zhang
authors.forename: Maosheng
authors.initials: M
authors.lastname: Cheng
authors.forename: Zhibing
authors.initials: Z
authors.lastname: Zheng
authors.forename: Keliang
authors.initials: K
authors.lastname: Liu
authors.forename: Junlin
authors.initials: J
authors.lastname: He
mesh_terms: D001483:Base Sequence; D002384:Catalysis; D020134:Catalytic Domain; D021881:DNA, Catalytic / Q000737:chemistry / Q000378:metabolism*; D006147:Guanine / Q000737:chemistry*; D007700:Kinetics
chemical_list: D021881:DNA, Catalytic; D006147:Guanine