title: [Effects of drug serum of plantaginis semen on bone formation function of rat osteoblast in vitro].
abstract: OBJECTIVE
  To study the influence of Plantaginis Semen on cell proliferation, differentiation and function of rat osteoblasts, and investigate the regulation effects of rat osteoblast epithelial sodium channel (ENaC) on bone formation.
  
  
  METHODS
  The animal serum was prepared by serum pharmacology means. The cells were got by separating and inducing the SD neonatal rat's skull bone. Cell proliferation and differentiation were evaluated by CCK-8 assay kit and AKP assay kit respectively. Regulation effects on mRNA expression of ENaC and osteogenesis gene were investigated by semi-quantitative PCR.
  
  
  RESULTS
  Plantaginis Semen stimulated the osteoblasts proliferation and differentiation,the difference between treatment group and control group had statistical significance (P < 0.01) in a dose-dependent manner. The effects of Plantaginis Semen serum on alpha-ENaC gene expression paralleled those on osteogenic gene (OC, ALP, OP) expression level.
  
  
  CONCLUSIONS
  Plantaginis Semen stimulates proliferation, differentiation and the mRNA expression of ENaC and osteogenesis gene in rat osteoblasts. Our results suggest that ENaC participate in the effects of Plantaginis Semen serum on osteoblast bone formation. Regulation of ENaC channel expression and function may provide a new clue for research on treatment of osteoporosis with traditional Chinese medicine.
authors.affiliation: School of Life Science and Biopharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Jia-Jian
authors.initials: JJ
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Lu
authors.forename: Guo-Zhu
authors.initials: GZ
authors.lastname: Yang
authors.forename: Xing-Yan
authors.initials: XY
authors.lastname: Lu
authors.forename: Jian
authors.initials: J
authors.lastname: Huang
authors.forename: Qing-Nan
authors.initials: QN
authors.lastname: Li
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology*; D053503:Epithelial Sodium Channels / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D010012:Osteogenesis; D010943:Plantago / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D012639:Seeds / Q000737:chemistry; D044967:Serum; D012886:Skull / Q000166:cytology
chemical_list: D004365:Drugs, Chinese Herbal; D053503:Epithelial Sodium Channels; D012333:RNA, Messenger; D000469:Alkaline Phosphatase