title: Binding of teicoplanin and vancomycin to bovine serum albumin in vitro: a multispectroscopic approach and molecular modeling.
abstract: In this paper, the binding properties of teicoplanin and vancomycin to bovine serum albumin (BSA) were investigated using fluorescence quenching, synchronous fluorescence, Fourier transform infrared (FTIR), circular dichroism (CD) and UV-vis spectroscopic techniques and molecular docking under simulative physiological conditions. The results obtained from fluorescence quenching data revealed that the drug-BSA interaction altered the conformational structure of BSA. Meanwhile, the 3D fluorescence, CD, FTIR and UV-vis data demonstrated that the conformation of BSA was slightly altered in the presence of teicoplanin and vancomycin, with different reduced α-helical contents. The binding distances for the drug-BSA system were provided by the efficiency of fluorescence resonance energy transfer (FRET). Furthermore, the thermodynamic analysis implied that hydrogen bond and van der Waals' forces were the main interaction for the drug-BSA systems, which agreed well with the results from the molecular modeling study. The results obtained herein will be of biological significance in future toxicological and pharmacological investigation.
authors.affiliation: Department of Orthopedics, the First Affiliated Hospital of Jinan University, Guangzhou, 510630, China.
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Lin
authors.forename: Genlong
authors.initials: G
authors.lastname: Jiao
authors.forename: Guodong
authors.initials: G
authors.lastname: Sun
authors.forename: Lili
authors.initials: L
authors.lastname: Zhang
authors.forename: Shilong
authors.initials: S
authors.lastname: Wang
authors.forename: Hanchao
authors.initials: H
authors.lastname: Liu
authors.forename: Zhizhong
authors.initials: Z
authors.lastname: Li
mesh_terms: D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002942:Circular Dichroism; D008958:Models, Molecular; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D017334:Teicoplanin / Q000737:chemistry*; D014640:Vancomycin / Q000737:chemistry*
chemical_list: D012710:Serum Albumin, Bovine; D017334:Teicoplanin; D014640:Vancomycin
keywords: bovine serum albumin; molecular docking; spectroscopy; teicoplanin; vancomycin