title: Expression of connexin 43 and E-cadherin protein and mRNA in non-small cell lung cancers in Chinese patients.
abstract: OBJECTIVE
  Connexin 43 (Cx43) and E-cadherin are important biomarkers related with cancer. Their expression at protein and mRNA levels was here investigated in 50 primary lung carcinoma tissues and 20 samples of adjacent normal tissue of Chinese patients with non-small cell lung cancer (NSCLC).
  
  
  METHODS
  Protein and mRNA expression were evaluated by ABC immunohistochemistry and RT-PCR.
  
  
  RESULTS
  (1) The positive expression rates of Cx43 and E-cadherin protein were higher in the adjacent normal tissues than those in the primary lung carcinoma tissues; (2) the positive expression rates of Cx43 and E-cadherin protein decreased with NSCLC progression; (3) the expression of E-cadherin protein was not related with the pathological type of NSCLC; and (4) the relative quantity of the Cx43 or E-cadherin mRNA expression was correlated with the the histological type, clinical stage, cancer cell differentiation and the lymph node metastasis.
  
  
  CONCLUSIONS
  The data suggested that the Cx43 and E-cadherin are reduced with NSCLC progression, and might be important biomarkers for judging the metastasis and prognosis.
authors.affiliation: First Affiliated Hospital, Zhengzhou University, Zhengzhou, China. bcd2009@126.com
authors.forename: Jun-Qiang
authors.initials: JQ
authors.lastname: Zhao
authors.forename: Fang-Jie
authors.initials: FJ
authors.lastname: Sun
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Yu-Quan
authors.initials: YQ
authors.lastname: Wu
authors.forename: Gui-Shan
authors.initials: GS
authors.lastname: Li
authors.forename: Qing-Yong
authors.initials: QY
authors.lastname: Chen
authors.forename: Jia-Xiang
authors.initials: JX
authors.lastname: Wang
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics; D015820:Cadherins / Q000096:biosynthesis* / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002454:Cell Differentiation; D002681:China; D018031:Connexin 43 / Q000096:biosynthesis* / Q000235:genetics; D018450:Disease Progression; D005260:Female; D015870:Gene Expression; D006801:Humans; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008207:Lymphatic Metastasis / Q000235:genetics / Q000473:pathology; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000096:biosynthesis
chemical_list: D014408:Biomarkers, Tumor; D015820:Cadherins; D018031:Connexin 43; C500216:GJA1 protein, human; D012333:RNA, Messenger