title: Antimicrobial activity of Leucas aspera engineered silver nanoparticles against Aeromonas hydrophila in infected Catla catla.
abstract: This research describes green synthesis of silver nanoparticles (AgNPs) utilizing Leucas aspera. The synthesized nanoparticles were characterized by UV-visible spectroscopy (UV-vis), dynamic light scattering (DLS), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR) and inductively coupled plasmon optical emission spectroscopy (ICP-OES). UV-vis analysis proved the wavelength of the sample to be 429 nm, resembling the surface resonance peak (SPR) specific for AgNPs. DLS analysis indicated particles with superior stability with an average diameter of 189.3 nm. TEM results showed that the particles were in the size range of 29-45 nm. FTIR prediction indicated the presence of possible polyphenol and protein encapsulates on the AgNPs. Antimicrobial activity of the AgNPs was tested against Aeromonas hydrophila. Catla catla, the model organism used for the experiment was divided into six groups with 15 animals in each group. In vivo analysis of biochemical parameters and histological architecture provided evidence for the antibacterial effect of AgNPs in the fish model.
authors.affiliation: Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli-24, Tamil Nadu, India.
authors.forename: Jacob Joe
authors.initials: JJ
authors.lastname: Antony
authors.forename: Murugaiyan
authors.initials: M
authors.lastname: Nivedheetha
authors.forename: Durairaj
authors.initials: D
authors.lastname: Siva
authors.forename: Ganesapandy
authors.initials: G
authors.lastname: Pradeepha
authors.forename: Palanivel
authors.initials: P
authors.lastname: Kokilavani
authors.forename: Seenivasan
authors.initials: S
authors.lastname: Kalaiselvi
authors.forename: Arunachalam
authors.initials: A
authors.lastname: Sankarganesh
authors.forename: Athmanathan
authors.initials: A
authors.lastname: Balasundaram
authors.forename: Vivekanandan
authors.initials: V
authors.lastname: Masilamani
authors.forename: Shanmugam
authors.initials: S
authors.lastname: Achiraman
mesh_terms: D016980:Aeromonas hydrophila / Q000187:drug effects*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology*; D002347:Carps / Q000382:microbiology*; D016905:Gram-Negative Bacterial Infections / Q000188:drug therapy*; D019686:Lamiaceae / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D010316:Particle Size; D010936:Plant Extracts / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D012834:Silver / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D013499:Surface Properties
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D012834:Silver