title: MiR-126 suppresses colon cancer cell proliferation and invasion via inhibiting RhoA/ROCK signaling pathway.
abstract: Recent data strongly suggests the profound role of miRNAs in cancer progression. Here, we showed miR-126 expression was much lower in HCT116, SW620 and HT-29 colon cancer cells with highly metastatic potential and miR-126 downregulation was more frequent in colorectal cancers with metastasis. Restored miR-126 expression inhibited HT-29 cell growth, cell-cycle progression and invasion. Mechanically, microarray results combined with bioinformatic and experimental analysis demonstrated miR-126 exerted cancer suppressor role via inhibiting RhoA/ROCK signaling pathway. These results suggest miR-126 function as a potential tumor suppressor in colon cancer progression and miR-126/RhoA/ROCK may be a novel candidate for developing rational therapeutic strategies.
authors.affiliation: Department of Gastroenterology, The Third Xiangya Hospital of Central South University, Tongzhipo Road 138#, Changsha, Hunan, People's Republic of China. linanxy3yy@csu.edu.cn
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.forename: Anliu
authors.initials: A
authors.lastname: Tang
authors.forename: Shuo
authors.initials: S
authors.lastname: Huang
authors.forename: Zeng
authors.initials: Z
authors.lastname: Li
authors.forename: Xiayu
authors.initials: X
authors.lastname: Li
authors.forename: Shourong
authors.initials: S
authors.lastname: Shen
authors.forename: Jian
authors.initials: J
authors.lastname: Ma
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Wang
mesh_terms: D015153:Blotting, Western; D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D053263:Gene Regulatory Networks; D045325:HCT116 Cells; D019073:HT29 Cells; D006801:Humans; D017403:In Situ Hybridization; D035683:MicroRNAs / Q000235:genetics*; D008957:Models, Genetic; D009361:Neoplasm Invasiveness; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics*; D054460:rho-Associated Kinases / Q000235:genetics* / Q000378:metabolism; D020742:rhoA GTP-Binding Protein / Q000235:genetics* / Q000378:metabolism
chemical_list: C527111:MIRN126 microRNA, human; D035683:MicroRNAs; C066737:RHOA protein, human; D054460:rho-Associated Kinases; D020742:rhoA GTP-Binding Protein