title: Characterization of migration parameters on peripheral and central nervous system T cells following treatment of experimental allergic encephalomyelitis with CRYAB.
abstract: CRYAB, a small heat shock protein, was previously shown to decrease neuroinflammation in experimental allergic encephalomyelitis (EAE). We investigated whether the expression of cell adhesion molecules and chemokine receptors on peripheral and spinal cord T cells, that could possibly affect their migration to the central nervous system, was altered following EAE CRYAB treatment. Less LFA-1+ lymphocytes and lower levels of iTAC, MCP-5 and MIG were observed in spinal cords of CRYAB-injected EAE animals. In addition, fewer blood T cells expressed CCR6, CXCR4 and CCR7 and in vivo-derived CRYAB EAE CD4+ lymphocytes were less migratory towards a MIP-3alpha gradient in vitro.
authors.affiliation: Department of Clinical Neurosciences, University of Calgary, Calgary, Alberta, Canada T2N 4N1.
authors.forename: Adeola I
authors.initials: AI
authors.lastname: Oyebamiji
authors.forename: Trisha M
authors.initials: TM
authors.lastname: Finlay
authors.forename: Rachael M
authors.initials: RM
authors.lastname: Hough
authors.forename: Vahid
authors.initials: V
authors.lastname: Hoghooghi
authors.forename: Erin-Mai F
authors.initials: EM
authors.lastname: Lim
authors.forename: Connie H Y
authors.initials: CH
authors.lastname: Wong
authors.forename: Shalina S
authors.initials: SS
authors.lastname: Ousman
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects* / Q000276:immunology; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D005260:Female; D008198:Lymph Nodes / Q000166:cytology; D051379:Mice; D057507:Mice, 129 Strain; D009103:Multiple Sclerosis / Q000188:drug therapy / Q000276:immunology / Q000473:pathology; D015213:Neuroimmunomodulation / Q000187:drug effects / Q000276:immunology; D054399:Receptors, CCR6 / Q000378:metabolism; D054400:Receptors, CCR7 / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000378:metabolism; D013116:Spinal Cord / Q000276:immunology / Q000473:pathology; D013154:Spleen / Q000166:cytology; D038203:alpha-Crystallin B Chain / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*
chemical_list: C498126:CCR6 protein, mouse; C514936:CXCR4 protein, mouse; C515371:Ccr7 protein, mouse; C525420:Cryab protein, mouse; D054399:Receptors, CCR6; D054400:Receptors, CCR7; D019718:Receptors, CXCR4; D038203:alpha-Crystallin B Chain