title: Human papillomavirus types 16, 18, and 31 share similar endocytic requirements for entry.
abstract: Human papillomavirus type 18 (HPV18), one of the HPVs with malignant potential, enters cells by an unknown endocytic mechanism. The key cellular requirements for HPV18 endocytosis were tested in comparison to those for HPV16 and -31 endocytoses. HPV18 (like HPV16 and -31) entry was independent of clathrin, caveolin, dynamin, and lipid rafts but required actin polymerization and tetraspanin CD151, and the viruses were routed to the same LAMP-1-positive compartment. Hence, the viruses shared similar cellular requirements for endocytic entry.
authors.affiliation: Department of Medical Microbiology and Hygiene, University Medical Centre of the Johannes Gutenberg University, Mainz, Germany.
authors.forename: Gilles
authors.initials: G
authors.lastname: Spoden
authors.forename: Lena
authors.initials: L
authors.lastname: Kühling
authors.forename: Nicole
authors.initials: N
authors.lastname: Cordes
authors.forename: Bettina
authors.initials: B
authors.lastname: Frenzel
authors.forename: Martin
authors.initials: M
authors.lastname: Sapp
authors.forename: Klaus
authors.initials: K
authors.lastname: Boller
authors.forename: Luise
authors.initials: L
authors.lastname: Florin
authors.forename: Mario
authors.initials: M
authors.lastname: Schelhaas
mesh_terms: D000199:Actins / Q000378:metabolism; D034285:Dynamin II; D004591:Electrophoresis, Polyacrylamide Gel; D004705:Endocytosis / Q000502:physiology*; D006367:HeLa Cells; D052162:Human papillomavirus 16 / Q000502:physiology*; D052161:Human papillomavirus 18 / Q000502:physiology*; D058952:Human papillomavirus 31 / Q000502:physiology*; D006801:Humans; D051907:Lysosomal Membrane Proteins / Q000378:metabolism; D021962:Membrane Microdomains; D018613:Microscopy, Confocal; D008854:Microscopy, Electron; D008856:Microscopy, Fluorescence; D058105:Polymerization; D060185:Tetraspanin 24 / Q000378:metabolism; D053586:Virus Internalization; D052118:Lysosomal-Associated Membrane Protein 1
chemical_list: D000199:Actins; D034285:Dynamin II; D051907:Lysosomal Membrane Proteins; D060185:Tetraspanin 24; C489116:LAMP1 protein, human; D052118:Lysosomal-Associated Membrane Protein 1