title: Supramolecular pseudo-block gene carriers based on bioreducible star polycations.
abstract: A series of supramolecular pseudo-block polycations (CD-SS-pDM/Ad-pPEGs) were realized by assembling bioreducible β-cyclodextrin-cored star poly (2-dimethyl amino)ethyl methacrylate with different molecular weight and an adamantine-ended linear poly(poly(ethylene glycol)ethyl ether methacrylate) (pPEGEEMA) via the host-guest interaction. The pseudo-block CD-SS-pDM/Ad-pPEG carriers were investigated in terms of DNA binding capability, cytotoxicity, gene transfection in HepG2 and COS7 cell lines, and in vivo anti-tumor activity. The pseudo-block carriers exhibited undiminished pDNA-condensing abilities compared with the starting star carriers. Meanwhile, the pseudo-block carriers displayed lower cytotoxicity and higher gene transfection efficiencies at various N/P ratios. These results are consistent with the favorable properties of pPEGEEMA as expected. Furthermore, cellular internalization results and in vivo anti-tumor activity analysis demonstrated that assembled pPEGEEMA could enhance the stability of pseudo-block carriers, thus improving their cellular internalization and gene transfection efficiency. The present study demonstrated that supramolecular pseudo-block polycations via the host-guest interaction is an effective means to produce new gene carriers.
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, College of Materials Science & Engineering, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Hu
authors.forename: Wei
authors.initials: W
authors.lastname: Yuan
authors.forename: Na-Na
authors.initials: NN
authors.lastname: Zhao
authors.forename: Jie
authors.initials: J
authors.lastname: Ma
authors.forename: Wan-Tai
authors.initials: WT
authors.lastname: Yang
authors.forename: Fu-Jian
authors.initials: FJ
authors.lastname: Xu
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D001672:Biocompatible Materials / Q000737:chemistry*; D019556:COS Cells; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D004247:DNA / Q000378:metabolism; D004121:Dimethyl Sulfoxide / Q000737:chemistry; D004705:Endocytosis / Q000187:drug effects; D005021:Ethylamines / Q000138:chemical synthesis / Q000737:chemistry*; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D007150:Immunohistochemistry; D009682:Magnetic Resonance Spectroscopy; D008689:Methacrylates / Q000138:chemical synthesis / Q000737:chemistry*; D051379:Mice; D018625:Microscopy, Atomic Force; D010316:Particle Size; D010957:Plasmids / Q000378:metabolism; D011073:Polyamines / Q000737:chemistry*; D000071228:Polyelectrolytes; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*; D055672:Static Electricity; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D047392:beta-Cyclodextrins / Q000138:chemical synthesis / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D001672:Biocompatible Materials; D004247:DNA; D004121:Dimethyl Sulfoxide; D005021:Ethylamines; D049452:Green Fluorescent Proteins; D008689:Methacrylates; D011073:Polyamines; D000071228:Polyelectrolytes; D011092:Polyethylene Glycols; D016159:Tumor Suppressor Protein p53; D047392:beta-Cyclodextrins; C510335:2-dimethylaminoethyl methacrylate; C485184:enhanced green fluorescent protein; C524499:polyethylene glycol methacrylate