title: [Tissue cultivation of tiller buds of Epimedium wushanense].
abstract: OBJECTIVE
  To established the rapid tissue propagation system of Epimedium wushanense, in order to provide theoretical basis for industrialized seed cultivation.
  
  
  METHODS
  Tiller buds E. wushanense were used as explants, with MS, B5, WPM as basic media, and added with different concentrations of plant growth regulators such as 6-BA, NAA and GA3, in order to conduct a systematic study on induction and propagation conditions for tiller buds.
  
  
  RESULTS
  The suitable method for sterilizing bud was to disinfect with 75% ethanol for 30 s, and then treated with 0.1% HgCl2 for (4 + 2) min for consecutively twice, which could control the pollution rate below 5% and the survival rate above 75%. The optimal medium for bud induction was WPM + 6-BA 2.0 mg x L(-1) + NAA 0.1 mg x L(-1) + GA3 0.5 mg x L(-1), with the induction rate of 75.5%; meanwhile, the basic medium and 6-BA showed significant effect on the induction rate. The propagation medium suitable for buds was MS +6-BA 2.0 mg x L(-1) + NAA 0.5 mg x L(-1), with the propagation rate of 3.3. The optimal growth of rooting medium was 1/2 WPM + IBA 0.5 mg x L(-1) + activated carbon which (0.05%), with the rooting rate of 90%, three to six strong seedlings in each plant.
  
  
  CONCLUSIONS
  The disinfection method suitable for tiller buds and the medium combination suitable for induction, propagation and rooting of adventitious buds are screened out to establish the rapid cultivation system for tiller buds of E. wushanense.
authors.affiliation: Institute of Chinese Medicinal, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Hai-Qin
authors.initials: HQ
authors.lastname: Zhou
authors.forename: Guo-Sheng
authors.initials: GS
authors.lastname: Zhu
authors.forename: Qiao-Sheng
authors.initials: QS
authors.lastname: Guo
authors.forename: Zuo-Yi
authors.initials: ZY
authors.lastname: Liu
authors.forename: Ning
authors.initials: N
authors.lastname: Zhou
mesh_terms: D034181:Epimedium / Q000187:drug effects / Q000254:growth & development*; D010937:Plant Growth Regulators / Q000494:pharmacology; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development*; D018547:Plant Stems / Q000187:drug effects / Q000254:growth & development*; D013997:Time Factors; D046509:Tissue Culture Techniques / Q000379:methods*
chemical_list: D010937:Plant Growth Regulators