title: [Morphology, immunohistochemistry and hTERC gene in-situ hybridization in Barrett's esophagus].
abstract: OBJECTIVE
  To study the clinicopathologic features and differential diagnosis of proximal gastric mucosa and mucosa of Barrett's esophagus (BE) in biopsy specimens.
  
  
  METHODS
  Thirty-eight cases of Barrett's esophagus (diagnosed using WHO criteria) and 44 cases of proximal gastric mucosa were studied by immunohistochemistry (for CK7, CK20, CK4, CK8, S-100 protein, MUC6, COX2 and bcl-2) and fluorescence in-situ hybridization (FISH) (for hTERC gene). The pathologic features were analyzed.
  
  
  RESULTS
  The differences in expression of CK7, CK20, MUC6, COX2 and bcl-2 between BE and proximal gastric mucosa with intestinal metaplasia were not statistically significant (P > 0.05). There was however a statistically significant difference in expression of S-100 protein (P < 0.05). The expression of CK7/CK4 and CK7/CK8 in BE showed positive correlation (P < 0.05). However, such correlation was not demonstrated in proximal gastric mucosa (P > 0.05). The results of hTERC gene expression by FISH showed a statistically significant difference between the two groups: 57.9% (22/38) in BE and 13.6% (6/44) in proximal gastric mucosa (P < 0.05).
  
  
  CONCLUSIONS
  The significance of CK7 and CK20 expression is uncertain in the differential diagnosis between BE and proximal gastric mucosa. On the other hand, positivity for CK7/CK4/CK8 may support the diagnosis of BE and play a role in distinguishing between the two groups. S-100 protein expression and detection of hTERC gene amplification also contribute to the diagnosis of BE.
authors.affiliation: Department of Pathology, General Hospital of Beijing Military Area, Beijing 100700, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.forename: Lu-ping
authors.initials: LP
authors.lastname: Wang
authors.forename: Sheng
authors.initials: S
authors.lastname: Xu
authors.forename: Guang-zhi
authors.initials: GZ
authors.lastname: Yang
mesh_terms: D001471:Barrett Esophagus / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D005753:Gastric Mucosa / Q000378:metabolism / Q000473:pathology; D005784:Gene Amplification; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D053554:Keratin-20 / Q000378:metabolism; D053527:Keratin-4 / Q000378:metabolism; D053552:Keratin-7 / Q000378:metabolism; D053533:Keratin-8 / Q000378:metabolism; D008679:Metaplasia / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D012313:RNA / Q000235:genetics*; D012189:Retrospective Studies; D009418:S100 Proteins / Q000378:metabolism*; D019098:Telomerase / Q000235:genetics*
chemical_list: D053554:Keratin-20; D053527:Keratin-4; D053552:Keratin-7; D053533:Keratin-8; D009418:S100 Proteins; C422952:telomerase RNA; D012313:RNA; D019098:Telomerase