title: Sensitivity and specificity of a commercial BSE kit for the detection of ovine scrapie.
abstract: To examine the sensitivity of a commercially available bovine spongiform encephalopathy (BSE) kit (NippIBL) for the detection of ovine scrapie, 50 scrapie-positive ovine samples from the UK, and 54 scrapie-negative ovine samples from Japan were obtain and tested using this kit. The sensitivity and specificity of NippIBL for ovine samples were 96% and 100%, respectively. The detection limit of the abnormal isoform of prion protein (PrP(Sc) ) of NippIBL was examined using diluted scrapie-positive samples. The sensitivity of NippIBL to ovine scrapie was 3-10 times superior to that of another commercial BSE diagnosis kit. Thus, the NippIBL kit proved more effective for the detection of ovine scrapie.
authors.affiliation: Nippi Research Institute of Biomatrix, Toride, Japan. t-yamamoto@nippi-inc.co.jp
authors.forename: Takuji
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Yuko
authors.initials: Y
authors.lastname: Ushiki-Kaku
authors.forename: Takashi
authors.initials: T
authors.lastname: Yokoyama
authors.forename: Shunji
authors.initials: S
authors.lastname: Hattori
mesh_terms: D000818:Animals; D002417:Cattle; D016643:Encephalopathy, Bovine Spongiform / Q000175:diagnosis*; D004797:Enzyme-Linked Immunosorbent Assay; D011328:Prions / Q000032:analysis; D011933:Reagent Kits, Diagnostic / Q000662:veterinary*; D012608:Scrapie / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D012756:Sheep
chemical_list: D011328:Prions; D011933:Reagent Kits, Diagnostic