title: Characterisation of an ELISA detecting immunoglobulin G to Mycobacterium avium subsp. paratuberculosis in bovine colostrum.
abstract: Although colostrum has been used to detect specific immunoglobulin (Ig) G to Mycobacterium avium subsp. paratuberculosis (MAP) in cattle, confounding, non-specific reactions can be a problem. The objectives of this study were to determine the proportion of non-specific ELISA reactions in samples of colostrum taken between 0 and 4 days-in-milk (DIM), and to assess the probability of an animal testing positive for MAP specific IgG over this time-period. Non-specific reactions were found in 3/365 (0.8%) of samples. The odds of an animal testing positive on day of calving were 130 times higher than at 4 DIM. The findings suggest colostral samples may have enhanced diagnostic potential over milk samples in determining if cattle have been exposed to or infected with MAP.
authors.affiliation: Department of Large Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Grønnegårdsvej 8, 1870 Frederiksberg C, Denmark.
authors.forename: Lisa Marie-Louise
authors.initials: LM
authors.lastname: Zervens
authors.forename: Søren Saxmose
authors.initials: SS
authors.lastname: Nielsen
authors.forename: Gregers
authors.initials: G
authors.lastname: Jungersen
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005260:Female; D007074:Immunoglobulin G / Q000737:chemistry*; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology / Q000302:isolation & purification*; D010283:Paratuberculosis / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D013045:Species Specificity
chemical_list: D007074:Immunoglobulin G
keywords: Cattle; Colostrum; ELISA; Mycobacterium avium subsp. paratuberculosis (MAP)