title: Escherichia coli bactofection using Lipofectamine.
abstract: Successful gene delivery into mammalian cells using bactofection requires entry of the bacterial vector via cell surface integrin receptors followed by release of plasmid DNA into the cellular environment. We show, for the first time, that addition of the DNA transfection reagent Lipofectamine improves entry of invasive Escherichia coli into HeLa cells and enhances up to 2.8-fold green fluorescent protein (GFP) expression from a reporter plasmid. The addition of Lipofectamine may be applicable to other bacterial vectors to increase their DNA delivery efficiency into mammalian cells.
authors.affiliation: School of Science, Monash University, 46150 Selangor Darul Ehsan, Malaysia. kumaran.narayanan@monash.edu
authors.forename: Kumaran
authors.initials: K
authors.lastname: Narayanan
authors.forename: Choon Weng
authors.initials: CW
authors.lastname: Lee
authors.forename: Aurelian
authors.initials: A
authors.lastname: Radu
authors.forename: Edmund Ui Hang
authors.initials: EU
authors.lastname: Sim
mesh_terms: D004926:Escherichia coli / Q000737:chemistry / Q000378:metabolism*; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008055:Lipids / Q000494:pharmacology*; D010957:Plasmids; D014162:Transfection / Q000379:methods*
chemical_list: D008055:Lipids; C086724:Lipofectamine; D049452:Green Fluorescent Proteins
keywords: Bactofection; DNA delivery; E. coli; Invasin; Lipofectamine; Transfection reagent