title: Translocation of cell penetrating peptides on Chlamydomonas reinhardtii.
abstract: Engineering of algal cells by delivering macromolecules through cell wall and plasma membrane presents many difficulties with the conventional methods. Recent research has shown that a new delivery method, namely cell penetrating peptide (CPP), has the ability to translocate into animal, plant, fungal, and bacterial cells. This study reports the apparent translocation of CPPs into algal cells of Chlamydomonas reinhardtii and the successful delivery of the conjugated fluorochrome. Although translocation efficiency was specific to each CPP studied, pVEC (peptide vascular endothelial cadherin) showed the highest translocation efficiency in comparison with penetratin (PEN), trans-activating transcriptional (TAT) peptide, and transportan (TRA). The maximum translocation of pVEC into the algal cell was reached in 15 min of incubation at 25°C. More importantly, translocation with pVEC demonstrated an absence of cytotoxicity. Thus, we suggested that pVEC is an attractive candidate for delivering macromolecules into algal cells for use in industrial applications.
authors.affiliation: Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea.
authors.forename: Arumuganainar
authors.initials: A
authors.lastname: Suresh
authors.forename: Yeu-Chun
authors.initials: YC
authors.lastname: Kim
mesh_terms: D015820:Cadherins; D002352:Carrier Proteins; D057846:Cell-Penetrating Peptides / Q000737:chemistry / Q000493:pharmacokinetics*; D016825:Chlamydomonas reinhardtii / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D016503:Drug Delivery Systems / Q000379:methods*; D016650:Fluorescein-5-isothiocyanate / Q000032:analysis / Q000737:chemistry / Q000493:pharmacokinetics; D018613:Microscopy, Confocal; D008956:Models, Chemical; D010455:Peptides; D014357:Trypsin
chemical_list: D015820:Cadherins; D002352:Carrier Proteins; D057846:Cell-Penetrating Peptides; D010455:Peptides; C437783:pVEC peptide; C414409:penetratin; D014357:Trypsin; D016650:Fluorescein-5-isothiocyanate
keywords: cell penetrating peptides; delivery vector; microalgae; pVEC; translocation