title: Toxicity study of isolated polypeptide from wool hydrolysate.
abstract: The cytotoxicity of wool polypeptide has been evaluated by both cell and animal models. Wool was dissolved in sodium hydroxide solution, the pH value of the solution was adjusted to 5.55 and the precipitate was harvested as wool polypeptide. The spray-dried polypeptide was collected as powders and characterized by SEM, FTIR and TG-DSC. The cell culturing results showed that wool polypeptide had no obvious negative effect on cell viability in vitro. Both acute oral toxicity and subacute 30-day oral toxicology studies showed that wool polypeptide had no influence on body weight, feed consumption, blood chemistry, and hematology at any dose levels. There were no treatment related findings on gross or detailed necroscopy, organ weights, organ/body weight ratios and histology. Our study indicated the absence of toxicity in wool polypeptide and supported its safe use as a food ingredient or drug carrier.
authors.affiliation: Institute of Textiles and Clothing, The Hong Kong Polytechnic University, Hong Kong.
authors.forename: Jiashen
authors.initials: J
authors.lastname: Li
authors.forename: Yi
authors.initials: Y
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xuan
authors.initials: X
authors.lastname: Liu
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhao
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Han
authors.forename: Dangxia
authors.initials: D
authors.lastname: Zhou
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001774:Blood Chemical Analysis; D001835:Body Weight / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004435:Eating / Q000187:drug effects; D005260:Female; D005347:Fibroblasts / Q000187:drug effects; D059022:Food Safety; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D008813:Mice, Inbred ICR; D064347:Microscopy, Electrochemical, Scanning; D010455:Peptides / Q000008:administration & dosage / Q000302:isolation & purification* / Q000633:toxicity*; D011208:Powders / Q000032:analysis / Q000494:pharmacology; D011492:Protein Hydrolysates / Q000008:administration & dosage / Q000633:toxicity; D017207:Rats, Sprague-Dawley; D017550:Spectroscopy, Fourier Transform Infrared; D059551:Toxicity Tests, Subacute; D014935:Wool / Q000737:chemistry*
chemical_list: D010455:Peptides; D011208:Powders; D011492:Protein Hydrolysates