title: The activity of class I, II, III and IV of alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase (ALDH) in brain cancer.
abstract: The brain being highly sensitive to the action of alcohol is potentially susceptible to its carcinogenic effects. Alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) are the main enzymes involved in ethanol metabolism, which leads to the generation of carcinogenic acetaldehyde. Human brain tissue contains various ADH isoenzymes and possess also ALDH activity. The purpose of this study was to compare the capacity for ethanol metabolism measured by ADH isoenzymes and ALDH activity in cancer tissues and healthy brain cells. The samples were taken from 62 brain cancer patients (36 glioblastoma, 26 meningioma). For the measurement of the activity of class I and II ADH isoenzymes and ALDH activity, the fluorometric methods were used. The total ADH activity and activity of class III and IV isoenzymes were measured by the photometric method. The total activity of ADH, and activity of class I ADH were significantly higher in cancer cells than in healthy tissues. The other tested classes of ADH and ALDH did not show statistically significant differences of activity in cancer and in normal cells. Analysis of the enzymes activity did not show significant differences depending on the location of the tumor. The differences in the activity of total alcohol dehydrogenase, and class I isoenzyme between cancer tissues and healthy brain cells might be a factor for metabolic changes and disturbances in low mature cancer cells and additionally might be a reason for higher level of acetaldehyde which can intensify the carcinogenesis.
authors.affiliation: Department of Biochemical Diagnostics, Medical University, Sklodowskiej-Curie 24 A, 15-276, Bialystok, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Laniewska-Dunaj
authors.forename: Wojciech
authors.initials: W
authors.lastname: Jelski
authors.forename: Karolina
authors.initials: K
authors.lastname: Orywal
authors.forename: Jan
authors.initials: J
authors.lastname: Kochanowicz
authors.forename: Robert
authors.initials: R
authors.lastname: Rutkowski
authors.forename: Maciej
authors.initials: M
authors.lastname: Szmitkowski
mesh_terms: D000426:Alcohol Dehydrogenase / Q000378:metabolism*; D000444:Aldehyde Dehydrogenase / Q000378:metabolism*; D001932:Brain Neoplasms / Q000201:enzymology*; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged
chemical_list: D007527:Isoenzymes; D000426:Alcohol Dehydrogenase; D000444:Aldehyde Dehydrogenase