title: Vesicle deposition and subsequent membrane-melittin interactions on different substrates: a QCM-D experiment.
abstract: Quartz crystal microbalance with dissipation (QCM-D) technique is one of the most effective methods to monitor the dynamic behaviors of a layer on a solid surface. Moreover, it has been reported recently that it is able to provide a fingerprint for the peptide-membrane interactions. In this work, QCM-D technique combined with computer simulations was employed to investigate the deposition and transformation of vesicles, as well as the subsequent membrane-melittin interactions on different substrates. A range of substrate surfaces, i.e. naked SiO2 without or with Au/polyelectrolyte coating, were produced. The nature of the substrate determined whether the adsorbed vesicles were present as a high-quality supported bilayer or an assembled vesicle matrix, which consequently influenced the membrane-melittin interactions. It was indicated by the related computer simulations that the lipid packing state of the membrane was a key factor to determine the mechanism of membrane-peptide interactions. Furthermore, this work might be a good example of the application of QCM-D for the exploration of membrane-active peptides.
authors.affiliation: Department of Physics, Nanjing University, Nanjing, People's Republic of China.
authors.forename: Nai-Yan
authors.initials: NY
authors.lastname: Lu
authors.forename: Kai
authors.initials: K
authors.lastname: Yang
authors.forename: Jing-Liang
authors.initials: JL
authors.lastname: Li
authors.forename: Bing
authors.initials: B
authors.lastname: Yuan
authors.forename: Yu-Qiang
authors.initials: YQ
authors.lastname: Ma
mesh_terms: D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism; D003198:Computer Simulation; D008051:Lipid Bilayers / Q000737:chemistry* / Q000378:metabolism; D008555:Melitten / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D058525:Quartz Crystal Microbalance Techniques; D012822:Silicon Dioxide / Q000378:metabolism*; D013379:Substrate Specificity; D013499:Surface Properties; D053835:Unilamellar Liposomes / Q000737:chemistry*
chemical_list: D008051:Lipid Bilayers; D053835:Unilamellar Liposomes; D008555:Melitten; D012822:Silicon Dioxide