title: An effective antibiofilm agent against Pseudomonas aeruginosa biofilm from traditional Thai herbal recipes used for wound treatments.
abstract: The presence of bacterial biofilm, particularly formed by Pseudomonas aeruginosa, has been considered an important factor responsible for wound chronicity. The objective of this study was to investigate the antibiofilm activity of water and ethanol extracts obtained from three traditional herbal recipes (THR-SK004, THR-SK010, and THR-SK011) on biofilm formation and on mature biofilm of a reference strain of P. aeruginosa. The effects of the extracts on the biofilm mass were evaluated by using crystal violet (CV) assay. The respiratory activity of preformed biofilm of P. aeruginosa after treatment with the extract was determined by MTT reduction assay. Scanning electron microscopy was used to furnish images of biofilm reduction after the recipe treatment. Tested ethanol extracts displayed antibiofilm activity, but the water extracts exhibited low biofilm inhibition activity at the tested concentrations. Remarkable reduction in biofilm formation of P. aeruginosa was found after treatment with the THR-SK010 ethanol extract (THR-SK010E). Treatments with this extract resulted in prevention of biofilm formation of P. aeruginosa on both polystyrene and glass surfaces. Almost 50% reduction in the bacterial metabolic activity in the preformed biofilm was seen after exposure to the extract-supplemented buffer for 12 hr. After a 24-hr treatment with THR-SK010E at 62.5 μg/ml, 97.3% of the preformed biofilms were destroyed. Promising antibiofilm activity was displayed by the THR-SK010 ethanol extract, suggesting further investigation to explore the possible utilization of the herbal recipe as an antibiofilm agent, especially for wound treatment.
authors.affiliation: 1 Faculty of Traditional Thai Medicine, Prince of Songkla University , Hat Yai, Songkhla, Thailand .
authors.forename: Sasitorn
authors.initials: S
authors.lastname: Chusri
authors.forename: Wittaya
authors.initials: W
authors.lastname: Jittanon
authors.forename: Katesarin
authors.initials: K
authors.lastname: Maneenoon
authors.forename: Supayang Piyawan
authors.initials: SP
authors.lastname: Voravuthikunchai
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D000431:Ethanol / Q000737:chemistry; D005898:Glass; D006801:Humans; D008519:Medicine, Traditional / Q000379:methods; D008826:Microbial Sensitivity Tests; D008855:Microscopy, Electron, Scanning; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011137:Polystyrenes; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000254:growth & development / Q000648:ultrastructure; D012997:Solvents / Q000737:chemistry; D013785:Thailand; D014867:Water / Q000737:chemistry; D014945:Wound Healing
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D011137:Polystyrenes; D012997:Solvents; D014867:Water; D000431:Ethanol