title: Enhancing HSP70-ShRNA transfection in 22RV1 prostate cancer cells by combination of sonoporation, liposomes and HTERT/CMV chimeric promoter.
abstract: Gene therapy is a potentially viable approach for treating hormone-refractory prostate cancer (HRPC), it requires efficient delivery systems and a target gene. Inducing carcinoma cell apoptosis by inhibition of heat shock protein 70 (HSP70) overexpression has been emerging as an attractive strategy for cancer therapy. In our study, the high tumor-specificity of human telomerase reverse transcriptase (HTERT) expression prompted the use of an HTERT/cytomegalovirus (CMV) chimeric promoter to drive HSP70-ShRNA expression to induce HRPC 22RV1 cell apoptosis. At the same time, sonoporation induced by ultrasound-targeted microbubble destruction (UTMD) was utilized for delivery of plasmid loaded with HTERT/CMV promoter. Our results indicated the combination of sonoporation, low-dose liposomes and HTERT/CMV chimeric promoter as a delivery system has the potential to promote efficient gene transfer with lower cytotoxicity.
authors.affiliation: Department of Ultrasound, Shanghai First People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200080, P.R. China.
authors.forename: Yun Hua
authors.initials: YH
authors.lastname: Li
authors.forename: Li Fang
authors.initials: LF
authors.lastname: Jin
authors.forename: Lian-Fang
authors.initials: LF
authors.lastname: Du
authors.forename: Qiu Sheng
authors.initials: QS
authors.lastname: Shi
authors.forename: Long
authors.initials: L
authors.lastname: Liu
authors.forename: Xiao
authors.initials: X
authors.lastname: Jia
authors.forename: Ying
authors.initials: Y
authors.lastname: Wu
authors.forename: Fan
authors.initials: F
authors.lastname: Li
authors.forename: Hang Hui
authors.initials: HH
authors.lastname: Wang
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D003587:Cytomegalovirus / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D015316:Genetic Therapy; D005822:Genetic Vectors; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics*; D006801:Humans; D008081:Liposomes / Q000008:administration & dosage; D008297:Male; D011401:Promoter Regions, Genetic; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology / Q000628:therapy; D034741:RNA, Small Interfering; D019098:Telomerase / Q000235:genetics; D014162:Transfection / Q000379:methods*
chemical_list: D018840:HSP70 Heat-Shock Proteins; D008081:Liposomes; D034741:RNA, Small Interfering; C509186:TERT protein, human; D019098:Telomerase