title: [Effect of chitosan oligosacharide on the inhibition and apoptosis of ovarian carcinoma SKOV-3 cells].
abstract: OBJECTIVE
  To explore the effect and mechanism of inhibition and apoptosis of SKOV-3 cells induced by Chitosan oligosaccharide.
  
  
  METHODS
  Human ovarian cancer SKOV-3 cells were treated by various concentrations of Chitosan oligosaccharide. The inhibition of cell proliferation was tested by MTT. Apoptosis induced by Chitosan oligosaccharide was analysed by flow cytometry and agarose gel SKOV-3 cells DNA ladder electrophoresis.
  
  
  RESULTS
  Chitosan oligosaccharide could efficiently inhibit SKOV-3 cells proliferation in a dose-dependent manner. Also, the significantly apoptosis of SKOV-3 cells induced by Chitosan oligosaccharide was confirmed through flew cytometry with Annexin V/PI double labels and agarose gel DNA ladder electrophoresis.
  
  
  CONCLUSIONS
  Chitosan oligosaccharide can efficiently inhibit SKOV-3 cells proliferation and induce its obviously apoptosis.
authors.affiliation: Binzhou Medical University, Yantai 264003, China. gzh3308@163.com
authors.forename: Zong-Hua
authors.initials: ZH
authors.lastname: Gao
authors.forename: Yao-Feng
authors.initials: YF
authors.lastname: Lv
authors.forename: Guan-Hua
authors.initials: GH
authors.lastname: Huo
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D048271:Chitosan / Q000008:administration & dosage / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D010051:Ovarian Neoplasms / Q000473:pathology*
chemical_list: D000970:Antineoplastic Agents; D048271:Chitosan