title: Functional polymorphisms in FAS and FASL contribute to risk of squamous cell carcinoma of the larynx and hypopharynx in a Chinese population.
abstract: Accumulating evidences indicate that the functional FAS-1377G>A, -670A>G and FASL-844T>C polymorphisms affect the risk of several kinds of cancers. However, their roles in the development of larynx and hypopharynx squamous cell carcinoma (SCC) were still unknown in the Chinese. In the current study, we examined whether these functional genetic variants were associated with the risk of larynx and hypopharynx squamous SCC in a Han Chinese population. The FAS and FASL polymorphisms were genotyped in 300 patients with laryngeal and hypopharyngeal SCC and 300 control subjects by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). Logistic regression analysis revealed that subjects carrying the FASL-844CT or TT genotype had a significantly decreased risk of developing laryngeal and hypopharyngeal SCC [odds ratio (OR)=0.69; 95% confidence interval (CI)=0.51-0.93; P=0.016; or, OR=0.41; 95% CI=0.20-0.86; P=0.009] compared with those carrying the CC genotype. Joint gene-smoking and gene-drinking effects were also observed, with the OR of CC genotype for smokers or drinkers were 5.15 (95%CI=3.24-8.97) or 12.52 (95%CI=7.31-22.47), respectively. Therefore, the FASL-844T>C polymorphism is associated with genetic susceptibility of developing laryngeal and hypopharyngeal SCC in a Han Chinese population.
authors.affiliation: Department of Thoracic Surgery, Zhejiang Cancer Research Institute, Zhejiang Province Cancer Hospital, Zhejiang Cancer Center, Hangzhou 310022, China.
authors.forename: Jiwen
authors.initials: J
authors.lastname: Wang
authors.forename: Jia
authors.initials: J
authors.lastname: Gao
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Li
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Gao
authors.forename: Li
authors.initials: L
authors.lastname: Peng
authors.forename: Dangui
authors.initials: D
authors.lastname: Yan
authors.forename: Lisheng
authors.initials: L
authors.lastname: Liu
authors.forename: Dongmei
authors.initials: D
authors.lastname: Li
authors.forename: Lili
authors.initials: L
authors.lastname: Wei
authors.forename: Jun
authors.initials: J
authors.lastname: Qi
authors.forename: Changchun
authors.initials: C
authors.lastname: Zhou
mesh_terms: D000368:Aged; D000428:Alcohol Drinking / Q000235:genetics; D044466:Asian People / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000208:ethnology / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D016001:Confidence Intervals; D055088:Early Detection of Cancer; D053222:Fas Ligand Protein / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease / Q000208:ethnology; D006801:Humans; D007012:Hypopharyngeal Neoplasms / Q000208:ethnology / Q000235:genetics*; D007013:Hypopharynx / Q000473:pathology; D007822:Laryngeal Neoplasms / Q000208:ethnology / Q000235:genetics* / Q000473:pathology; D007830:Larynx / Q000473:pathology; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D012907:Smoking / Q000235:genetics; D019014:fas Receptor / Q000235:genetics*
chemical_list: C491901:FAS protein, human; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D019014:fas Receptor