title: Competitive adsorption-desorption of IgM monomers-dimers on silica and modified silica surfaces.
abstract: Understanding competitive adsorption-desorption of proteins onto surfaces is an important area of research in food processing and biomedical engineering. Here, we demonstrate, how electrospray-differential mobility analysis that has been traditionally used for characterizing bionanoparticles, can be used for quantifying complex competitive adsorption-desorption of oligomeric proteins or multiprotein systems using monomers and dimers of IgM as a model example onto silica and modified silica surfaces. Using ES-DMA, we show that IgM dimers show a preference to stay adsorbed to different surfaces although monomers adsorb more easily and desorption rates of monomers and dimers of IgM are surface-type-dependent and are not significantly affected by shear. We anticipate that this demonstration will make ES-DMA a popular "label-free" method for studying multicomponent multi-oligomeric protein adsorption to different surfaces in the future.
authors.affiliation: University of Maryland, College Park, MD 20742, United States.
authors.forename: Suvajyoti
authors.initials: S
authors.lastname: Guha
authors.forename: Joshua
authors.initials: J
authors.lastname: Wayment
authors.forename: Vinayak
authors.initials: V
authors.lastname: Rastogi
authors.forename: Mingdong
authors.initials: M
authors.lastname: Li
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Tarlov
authors.forename: Michael R
authors.initials: MR
authors.lastname: Zachariah
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D007075:Immunoglobulin M / Q000737:chemistry*; D055503:Protein Multimerization; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D007075:Immunoglobulin M; D012822:Silicon Dioxide