title: A rapid DNA assembling strategy mediated by direct full-length polymerase chain reaction.
abstract: An efficient DNA assembling strategy was developed here modified from Class-IIS endonuclease mediated DNA splicing by directed ligation (SDL). Benefited from the full-length PCR directly using ligation products as template, this strategy required less effort and less time to obtain the assembled full-length DNA. The advantages of this strategy made it a rapid and easy-to-perform gene splicing and multiple site-directed mutagenesis approach especially practicable when more fragments need to be assembled at the same time.
authors.affiliation: The SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, the MOE Key Laboratory for Standardization of Chinese Medicines and Shanghai Key Laboratory of Complex Prescription, Institute of Chinese Material Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Lu-Bin
authors.initials: LB
authors.lastname: Zhou
authors.forename: Qing-Qing
authors.initials: QQ
authors.lastname: Lin
authors.forename: Jing-Xian
authors.initials: JX
authors.lastname: Zhang
authors.forename: Shu-Juan
authors.initials: SJ
authors.lastname: Zhao
authors.forename: Zhi-Bi
authors.initials: ZB
authors.lastname: Hu
mesh_terms: D001234:Aspergillus niger / Q000235:genetics; D003001:Cloning, Molecular / Q000379:methods*; D018076:DNA, Complementary; D023061:Gene Order; D016681:Genome, Fungal; D016133:Polymerase Chain Reaction
chemical_list: D018076:DNA, Complementary