title: Lack of association between CTLA-4 and PDCD1 polymorphisms and acute rejection in German liver transplant recipients.
abstract: Cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) and programmed cell-death 1 (PDCD1) are two genes encoding coinhibitory immunoreceptors that are involved in regulation transplant rejection and tolerance induction. Thus, CTLA-4 and PDCD1 may be good candidate genes to evaluate in liver transplant rejection. In this retrospective study, we investigated whether four functional single nucleotide polymorphisms (SNP) of the CTLA-4 gene and PDCD1 gene were associated with susceptibility to liver transplant rejection. The SNPs -1772T>C (rs733618), -1661A>G (rs4553808) of the CTLA-4 gene, and the SNPs 7146G>A (rs11568821), 7209C>T (rs41386349) of the PDCD1 gene were genotyped by polymerase chain reaction allele specific restriction enzyme analysis (PCR-ASRA) in 100 liver recipients with acute rejection, 104 liver transplant recipients without acute rejection and 100 healthy control individuals. For the selected SNPs we did not detect any significant difference in genotypic and allelic frequencies between liver transplant recipients with and without acute rejection. In conclusion, our results suggest that the tested SNPs may not be associated with susceptibility to acute liver transplant rejection in a Caucasian population.
authors.affiliation: University Hospital Hamburg-Eppendorf, Department of Hepatobiliary and Transplant Surgery, Martinistrasse 52, 20246 Hamburg, Germany. h.thude@uke.de
authors.forename: Hansjörg
authors.initials: H
authors.lastname: Thude
authors.forename: Anna Dorothea
authors.initials: AD
authors.lastname: Schipler
authors.forename: Andras
authors.initials: A
authors.lastname: Treszl
authors.forename: Sven
authors.initials: S
authors.lastname: Peine
authors.forename: Martina
authors.initials: M
authors.lastname: Koch
authors.forename: Martina
authors.initials: M
authors.lastname: Sterneck
authors.forename: Björn
authors.initials: B
authors.lastname: Nashan
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D060908:CTLA-4 Antigen / Q000235:genetics* / Q000276:immunology; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D005858:Germany; D006084:Graft Rejection / Q000235:genetics* / Q000276:immunology; D006801:Humans; D016031:Liver Transplantation; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D020641:Polymorphism, Single Nucleotide; D061026:Programmed Cell Death 1 Receptor / Q000235:genetics*; D012189:Retrospective Studies; D044465:White People / Q000235:genetics*
chemical_list: D060908:CTLA-4 Antigen; D061026:Programmed Cell Death 1 Receptor
keywords: AZA; CD; CNI; CTLA-4; Cl; HBV; HCV; LTX; MMF; OR; PCR; PCR-ASRA; PDCD1; SD; SNP; azathioprine; base pairs; bp; calcineurin inhibitor; cluster of differentiation; confidence interval; cytotoxic T-lymphocyte-associated antigen 4; f; female; hepatitis B virus; hepatitis C virus; liver transplantation; m; mTOR; male; mammalian of rapamycin; mycophenolate mofetil; odds ratio; polymerase chain reaction; polymerase chain reaction-allele specific restriction enzyme analysis; programmed cell-death 1; sCTLA-4; single nucleotide polymorphism; soluble cytotoxic T-lymphocyte-associated antigen 4; standard deviation