title: Amniotic membrane extract-loaded double-layered wound dressing: evaluation of gel properties and wound healing.
abstract: The conservative single-layered wound dressing system is decomposed when mixed in polyvinyl alcohol (PVA) solution, which means it cannot be used with a temperature-sensitive drug. The goal of this investigation was to make an amniotic membrane extract (AME)-loaded double-layered wound dressing with an improved healing result compared to the conservative single-layered wound dressing systems. The double-layered wound dressing was developed with PVA/sodium alginate using a freeze-melting technique; one layer was PVA layer and the other was the drug-loaded sodium alginate layer. Its gel properties were assessed compared to single-layered wound dressings. Moreover, in vivo wound-healing effects and histopathology were calculated compared to commercial products. The double-layered wound dressing gave a similar gel fraction and Young's module as single-layered wound bandages developed with only PVA, and a similar inflammation ability and WVTR as single-layered wound dressings developed with PVA and sodium alginate. Our data indicate that these double-layered wound bandages were just as swellable, but more elastic and stronger than single-layered wound dressings comprised of the same polymers and quantities, possibly giving an acceptable level of moisture and accumulation of exudates in the wound zone. Compared to the commercial product, the double-layered wound dressing comprising 6.7% PVA, 0.5% sodium alginate and 0.01% AME significantly enhanced the wound-healing effect in the wound-healing test. Histological investigations showed that superior full-thickness wound-healing effects compared to the commercial product. Therefore, the double-layered wound dressing would be an outstanding wound-dressing system with improved wound healing and good gel property.
authors.affiliation: College of Pharmacy, Yeungnam University , Gyongsan , South Korea .
authors.forename: Yeung Keun
authors.initials: YK
authors.lastname: Choi
authors.forename: Fakhar Ud
authors.initials: FU
authors.lastname: Din
authors.forename: Dong Wuk
authors.initials: DW
authors.lastname: Kim
authors.forename: Yong-Il
authors.initials: YI
authors.lastname: Kim
authors.forename: Jong Oh
authors.initials: JO
authors.lastname: Kim
authors.forename: Sae Kwang
authors.initials: SK
authors.lastname: Ku
authors.forename: Jeong-Chan
authors.initials: JC
authors.lastname: Ra
authors.forename: Jae-Wook
authors.initials: JW
authors.lastname: Huh
authors.forename: Jangik I
authors.initials: JI
authors.lastname: Lee
authors.forename: Dong Hwan
authors.initials: DH
authors.lastname: Sohn
authors.forename: Chul Soon
authors.initials: CS
authors.lastname: Yong
authors.forename: Han-Gon
authors.initials: HG
authors.lastname: Choi
mesh_terms: D000464:Alginates / Q000737:chemistry; D000650:Amnion / Q000737:chemistry*; D000818:Animals; D001684:Biological Dressings; D004195:Disease Models, Animal; D005782:Gels; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D008297:Male; D064347:Microscopy, Electrochemical, Scanning; D011142:Polyvinyl Alcohol / Q000737:chemistry; D017207:Rats, Sprague-Dawley; D012996:Solutions; D013499:Surface Properties; D013718:Tensile Strength; D014020:Tissue Extracts / Q000008:administration & dosage / Q000737:chemistry* / Q000627:therapeutic use; D014945:Wound Healing / Q000187:drug effects*; D014950:Wounds, Penetrating / Q000188:drug therapy* / Q000473:pathology
chemical_list: D000464:Alginates; D005782:Gels; D006603:Hexuronic Acids; D012996:Solutions; D014020:Tissue Extracts; D020723:Glucuronic Acid; D011142:Polyvinyl Alcohol
keywords: Amniotic membrane extract; double-layered wound dressing; polyvinyl alcohol; sodium alginate; wound healing effect