title: Hypoxia increases serum amyloid A3 (SAA3) in differentiated 3T3-L1 adipocytes.
abstract: Hypoxia has been implicated as a possible cause of adipose tissue inflammation. Furthermore, the acute phase protein serum amyloid A (SAA) has been associated with the modulation of the adipogenic process, and it is well-known that obese individuals have increased levels of SAA. The effect of hypoxia in the expression and production of SAA was examined in murine 3T3-L1 adipocytes. Hypoxia leads to a substantial increase in SAA3 mRNA and protein level, apparently in a time-dependent manner (threefold in 48 h), in fully differentiated 3T3-L1, followed by reestablishment of gene expression to basal levels after 24 h of reoxygenation. Hypoxia-induced SAA may be one of the key molecules to the development of the inflammatory response in adipose tissue.
authors.affiliation: Department of Clinical and Toxicological Analysis, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580, São Paulo, CEP 05508-900, Brazil, edsonmendes@usp.br.
authors.forename: Edson Mendes
authors.initials: EM
authors.lastname: de Oliveira
authors.forename: Silvana
authors.initials: S
authors.lastname: Sandri
authors.forename: Franciele Hinterholz
authors.initials: FH
authors.lastname: Knebel
authors.forename: Caroline Garcia Iglesias
authors.initials: CG
authors.lastname: Contesini
authors.forename: Ana
authors.initials: A
authors.lastname: Campa
authors.forename: Fabíola Branco
authors.initials: FB
authors.lastname: Filippin-Monteiro
mesh_terms: D016475:3T3 Cells; D017667:Adipocytes / Q000378:metabolism*; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D002454:Cell Differentiation; D015687:Cell Hypoxia; D002460:Cell Line; D002470:Cell Survival; D007249:Inflammation / Q000378:metabolism; D051379:Mice; D009765:Obesity / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis; D000685:Serum Amyloid A Protein / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; C076498:Saa3 protein, mouse; D000685:Serum Amyloid A Protein