title: A new monoclonal anti-human CD83 antibody for flow cytometry, western blot analysis, and ELISA.
abstract: CD83 is expressed on mature dendritic cells (DCs) and many immune cells, including B and T lymphocytes upon activation. CD83 is essential for the development of CD4 single positive T cells and involved in the regulation of peripheral T and B cell responses. Here we generated a new monoclonal antibody against human CD83, which we named ELBE-1. ELBE-1 recognizes a linear epitope and cannot only be applied for Western blot analysis and flow cytometry, but also detects soluble CD83 (sCD83) by ELISA. As with other anti-CD83 antibodies described thus far, ELBE-1 does not influence T cell activation. Nevertheless, it represents a useful new tool for the detection and functional characterization of CD83.
authors.affiliation: Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Papp
authors.forename: Ulricke
authors.initials: U
authors.lastname: Richardt
authors.forename: Bernhard
authors.initials: B
authors.lastname: Fleischer
authors.forename: Anke
authors.initials: A
authors.lastname: Osterloh
mesh_terms: D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000737:chemistry* / Q000276:immunology; D015703:Antigens, CD / Q000276:immunology*; D015153:Blotting, Western; D019556:COS Cells; D049109:Cell Proliferation; D002522:Chlorocebus aethiops; D018920:Coculture Techniques; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping; D061252:Feeder Cells; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D006825:Hybridomas; D007136:Immunoglobulins / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D013601:T-Lymphocytes / Q000502:physiology; D000097802:CD83 Antigen
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D015703:Antigens, CD; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D000097802:CD83 Antigen