title: In vitro antiproliferative effect of a water-soluble Laminaria japonica polysaccharide on human melanoma cell line A375.
abstract: A water-soluble polysaccharide WPS-2-1, purified from Laminaria japonica, has been found to have antitumor activity. In this study, WPS-2-1 exhibited high anti-proliferative activity on A375 cells in a dosedependent manner. Further investigation indicated that WPS-2-1 induced A375 cells apoptosis. Moreover, WPS-2-1-induced apoptosis was associated with the alteration in expressions of Bcl-2 family proteins. Mitochonadrial apoptotic pathway was involved in WPS-2-1-induced apoptosis, which included the loss of mitochondrial membrane and activation of caspase-3/9. The results in this study suggested that WPS-2-1 could effectively inhibit proliferation of A375 cells in vitro and induce apoptosis via mitochondrial apoptotic pathway. It might serve as a potential antitumor agent.
authors.affiliation: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou, China.
authors.forename: Zhenfei
authors.initials: Z
authors.lastname: Peng
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.forename: Zhexiang
authors.initials: Z
authors.lastname: Fang
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.forename: Jiulin
authors.initials: J
authors.lastname: Wu
authors.forename: Qiqing
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D007795:Laminaria / Q000737:chemistry*; D008545:Melanoma / Q000378:metabolism / Q000473:pathology*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D011134:Polysaccharides / Q000494:pharmacology*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D011134:Polysaccharides; D051028:bcl-2-Associated X Protein
keywords: A375 cells; Antitumor activity; Apoptosis; Bcl-2 family proteins; Laminaria japonica polysaccharide