title: DNA replication depends on photosynthetic electron transport in cyanobacteria.
abstract: The freshwater cyanobacterium Synechococcus elongatus PCC 7942 exhibits light-dependent growth. Although it has been reported that DNA replication also depends on light irradiation in S. elongatus 7942, the involvement of the light in the regulation of DNA replication remains unclear. To elucidate the regulatory pathway of DNA replication by light, we studied the effect of several inhibitors, including two electron transport inhibitors, 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) and 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB), on DNA replication in S. elongatus 7942. DCMU inhibited only DNA replication initiation, whereas DBMIB blocked both the initiation and progression of DNA replication. These results suggest that DNA replication depends on the photosynthetic electron transport activity and initiation and progression of DNA replication are regulated in different ways.
authors.affiliation: Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan.
authors.forename: Ryudo
authors.initials: R
authors.lastname: Ohbayashi
authors.forename: Satoru
authors.initials: S
authors.lastname: Watanabe
authors.forename: Yu
authors.initials: Y
authors.lastname: Kanesaki
authors.forename: Rei
authors.initials: R
authors.lastname: Narikawa
authors.forename: Taku
authors.initials: T
authors.lastname: Chibazakura
authors.forename: Masahiko
authors.initials: M
authors.lastname: Ikeuchi
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Yoshikawa
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D004261:DNA Replication / Q000528:radiation effects; D004579:Electron Transport; D015964:Gene Expression Regulation, Bacterial / Q000528:radiation effects; D008027:Light; D010788:Photosynthesis; D046940:Synechococcus / Q000235:genetics* / Q000378:metabolism* / Q000528:radiation effects
chemical_list: D001426:Bacterial Proteins