title: Highly H2O2-sensitive electrospun quantum dots nanocomposite films for fluorescent biosensor.
abstract: Bright CdSe quantum dots (QDs)/polycaprolactone (PCL) nanocomposite fluorescent films were fabricated by electronspinning. By using chloroform and N,N-dimethylformamide as electronspinning solvent, the oil-soluble CdSe QDs were uniformly distributed in PCL fibers, and were directly employed as optical probe without any modification processing. The fluorescences of CdSe QDs/PCL nanocomposite films were quickly quenched when the films were contacted with H2O2, solution. In the presence of glucose oxidase (GOD), the fluorescence intensities of these fluorescent films exhibit a liner change with the concentrations of glucose. The H2O2-sensitive electrospun QDs nanocomposite films are highly uniform and repeatable, demonstrating the potential to fabricate stable, sensitive and recyclable fluorescent biosensor for the detection different H2O2-generating oxidases and their substrates.
authors.affiliation: Laboratory of Controllable Preparation and Application of Nanomaterials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, People's Republic of China.
authors.forename: Longfei
authors.initials: L
authors.lastname: Tan
authors.forename: Xiaolong
authors.initials: X
authors.lastname: He
authors.forename: Dong
authors.initials: D
authors.lastname: Chen
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wu
authors.forename: Hongbo
authors.initials: H
authors.lastname: Li
authors.forename: Xiangling
authors.initials: X
authors.lastname: Ren
authors.forename: Xianwei
authors.initials: X
authors.lastname: Meng
authors.forename: Fangqiong
authors.initials: F
authors.lastname: Tang
mesh_terms: D015374:Biosensing Techniques; D019187:Cadmium Compounds / Q000138:chemical synthesis / Q000737:chemistry; D055664:Electrochemical Techniques / Q000295:instrumentation*; D005453:Fluorescence; D005949:Glucose Oxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D011091:Polyesters / Q000138:chemical synthesis / Q000737:chemistry; D045663:Quantum Dots; D018036:Selenium Compounds / Q000138:chemical synthesis / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: D019187:Cadmium Compounds; D011091:Polyesters; D018036:Selenium Compounds; C016240:polycaprolactone; C058667:cadmium selenide; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase