title: Altered expression of β-catenin, E-cadherin, and E-cadherin promoter methylation in epithelial ovarian carcinoma.
abstract: The aim of the study was to evaluate the immunoexpression of E-cadherin, β-catenin, and Ki-67, as well as the promoter methylation of E-cadherin gene in epithelial ovarian cancer (EOC), as well as to find a possible relationship between the immunoexpression and hypermethylation. Promoter methylation was studied using methylation-specific PCR in 86 malignant cases, 14 low malignant potential (LMP) tumors and 19 benign cystadenomas. Immunohistochemical expression was carried out in 64 malignant cases, 8 LMP tumors, and 11 benign cystadenomas. Immunoexpression of E-cadherin was reduced in EOC, while 100 % expression was seen in LMP tumors and benign cystadenomas. An interesting observation was the nuclear expression of E-cadherin in a high percentage of cancers, which showed a positive correlation with Ki-67. Β-Catenin expression showed heterogeneous localization with increased nuclear localization, which was significantly higher in cases that did not express E-cadherin. Promoter methylation of E-cadherin was 36, 14, and 11 % in EOC, LMP tumors, and benign cystadenomas, respectively. Our results suggest that reduced expression of E-cadherin is associated with promoter methylation of E-cadherin gene, in addition to providing evidence for the aberrant nuclear localization of E-cadherin in EOC.
authors.affiliation: Department of Biochemistry, Kidwai Memorial Institute of Oncology, Dr. M. H. Marigowda Road, Bangalore, 560029, India. rahulbhagat1234@gmail.com
authors.forename: Rahul
authors.initials: R
authors.lastname: Bhagat
authors.forename: C S
authors.initials: CS
authors.lastname: Premalata
authors.forename: V
authors.initials: V
authors.lastname: Shilpa
authors.forename: V R
authors.initials: VR
authors.lastname: Pallavi
authors.forename: G
authors.initials: G
authors.lastname: Ramesh
authors.forename: C R
authors.initials: CR
authors.lastname: Vijay
authors.forename: Lakshmi
authors.initials: L
authors.lastname: Krishnamoorthy
mesh_terms: D015820:Cadherins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism*; D000077216:Carcinoma, Ovarian Epithelial; D002467:Cell Nucleus / Q000378:metabolism; D019175:DNA Methylation; D005260:Female; D006801:Humans; D019394:Ki-67 Antigen / Q000096:biosynthesis / Q000378:metabolism*; D009375:Neoplasms, Glandular and Epithelial / Q000235:genetics / Q000378:metabolism*; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D051176:beta Catenin / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*
chemical_list: D015820:Cadherins; D019394:Ki-67 Antigen; D051176:beta Catenin