title: Early increase in alveolar macrophage prostaglandin 15d-PGJ2 precedes neutrophil recruitment into lungs of cytokine-insufflated rats.
abstract: Early detection and prevention is an important goal in acute respiratory distress syndrome research. We determined the concentration of the anti-inflammatory 15-deoxy-Δ(12,14)-prostaglandin-J2 (15d-PGJ2) and other components of the cyclopentenone prostaglandin cascade in relation to lung inflammation in cytokine (IL-1/LPS)-insufflated rats. We found that 15d-PGJ2 levels increase in the bronchoalveolar lavage (BAL) fluid of rats insufflated with cytokines 2 h before. BAL 15d-PGJ2 increases preceded neutrophil recruitment, lung injury, and oxidative stress in the lungs of cytokine-insufflated rats. 15d-PGJ2 was localized in alveolar macrophages that decreased following cytokine insufflation. 15d-PGJ2 may constitute an early biomarker of lung inflammation and may reflect an endogenous attempt to regulate ongoing inflammation in macrophages and elsewhere after cytokine insufflation.
authors.affiliation: Department of Anesthesiology, University of Colorado SOM, AO-1, MS 8202, 12631 E 17th Ave, Aurora, CO, 80045, USA, Ana.Fernandez-Bustamante@ucdenver.edu.
authors.forename: Ana
authors.initials: A
authors.lastname: Fernandez-Bustamante
authors.forename: Jelena
authors.initials: J
authors.lastname: Klawitter
authors.forename: Paul
authors.initials: P
authors.lastname: Wilson
authors.forename: Nancy D
authors.initials: ND
authors.lastname: Elkins
authors.forename: Amanda
authors.initials: A
authors.lastname: Agazio
authors.forename: Takahiro
authors.initials: T
authors.lastname: Shibata
authors.forename: Koji
authors.initials: K
authors.lastname: Uchida
authors.forename: Uwe
authors.initials: U
authors.lastname: Christians
authors.forename: John E
authors.initials: JE
authors.lastname: Repine
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology*; D002452:Cell Count; D016207:Cytokines / Q000008:administration & dosage; D007249:Inflammation; D007327:Insufflation; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008070:Lipopolysaccharides; D008168:Lung / Q000166:cytology / Q000276:immunology; D016676:Macrophages, Alveolar / Q000378:metabolism*; D020556:Neutrophil Infiltration / Q000276:immunology*; D018384:Oxidative Stress; D015230:Prostaglandin D2 / Q000031:analogs & derivatives* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012128:Respiratory Distress Syndrome / Q000175:diagnosis / Q000378:metabolism*
chemical_list: C477819:15-deoxyprostaglandin J2; D015415:Biomarkers; D016207:Cytokines; D008070:Lipopolysaccharides; D007770:L-Lactate Dehydrogenase; D015230:Prostaglandin D2