title: Isolation and characterization of biologically active melanin from Actinoalloteichus sp. MA-32.
abstract: Melanins are enigmatic pigments and biological macromolecules that are produced by a wide variety of microorganisms including several species of bacteria and fungi. The present study was carried out on isolation and characterization of melanin from marine actinobacteria, Actinoalloteichus sp. MA-32. Medium composition and culture conditions for the melanin production by Actinoalloteichus sp. MA-32 were optimized using two statistical methods: Plackett-Burman design applied to find the key ingredients and conditions for the best yield of melanin production and central composite design used to optimize the concentration of the four significant variables: glycerol, L-tyrosine, NaCl and trace salt solution. The melanin was optimally active at pH 7-9 and temperature 45-60°C and it was most stable up to pH 11 and 4% of NaCl concentration. Melanin was examined by UV-vis absorption spectroscopy and infrared spectrometry. Melanin has potential antibacterial activity as it showed greater antagonistic and it has a strong antioxidant potential observed in the in vitro evaluation of its DPPH radical-scavenging activity, superoxide radical-scavenging activity, nitric oxide-scavenging activity, reducing power and metal chelating activity. The observed activities indicate that melanin might be a novel potential antioxidant. This study suggested that the melanin could potentially be used as a natural antioxidant in the food, cosmetic and pharmaceutical industries.
authors.affiliation: Marine Biotechnology Laboratory, Department of Chemistry and Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, Republic of Korea.
authors.forename: Panchanathan
authors.initials: P
authors.lastname: Manivasagan
authors.forename: Jayachandran
authors.initials: J
authors.lastname: Venkatesan
authors.forename: Kalimuthu
authors.initials: K
authors.lastname: Senthilkumar
authors.forename: Kannan
authors.initials: K
authors.lastname: Sivakumar
authors.forename: Se-Kwon
authors.initials: SK
authors.lastname: Kim
mesh_terms: D000192:Actinomycetales / Q000737:chemistry* / Q000235:genetics; D000900:Anti-Bacterial Agents / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D059001:Aquatic Organisms / Q000737:chemistry / Q000235:genetics; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000737:chemistry; D002614:Chelating Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008543:Melanins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D010084:Oxidation-Reduction; D010802:Phylogeny; D010851:Picrates / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019163:Reducing Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D054712:Salinity; D013481:Superoxides / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D001713:Biphenyl Compounds; D002614:Chelating Agents; D016166:Free Radical Scavengers; D008543:Melanins; D010851:Picrates; D012336:RNA, Ribosomal, 16S; D019163:Reducing Agents; D013481:Superoxides; C004931:1,1-diphenyl-2-picrylhydrazyl