title: [The effect of eukaryotic expression plasmid ARHI on gastric cancer].
abstract: OBJECTIVE
  To study the effect of expressed aplasia ras homolog member I (ARHI) on the malignant biological behaviors of gastric cancer including the proliferation, migration and invasion of the cell.
  
  
  METHODS
  The eukaryotic expression plasmid of ARHI was constructed and transfected into MKN-28 cell with lipofectamine 2000 as pEGFP-ARHI group, transfected with pEGFP-N1 as pEGFP-N1 group, and untreated MKN-28 as control group. The expression of ARHI was detected by Western blotting and fluorescence microscope. CCK-8 assay was used to analyze the cell proliferation, the wound-healing assay and transwell assay were performed to investigate the effects on migration and invasion.
  
  
  RESULTS
  Compared with the pEGFP-N1 group and control group, proliferation, invasion and migration of the pEGFP-ARHI group were depressed (P < 0.05).
  
  
  CONCLUSIONS
  Recombination eukaryotic expression pEGFP-ARHI could partially reverse the malignant phenotypes of gastric cancer cell MKN-28.
authors.affiliation: Department of Abdominal Cancer, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Li-Bo
authors.initials: LB
authors.lastname: Li
authors.forename: Qiu-Lin
authors.initials: QL
authors.lastname: Tang
authors.forename: Ming-Xing
authors.initials: MX
authors.lastname: Li
authors.forename: Wei-Bing
authors.initials: WB
authors.lastname: Leng
authors.forename: Liu
authors.initials: L
authors.lastname: Chen
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Yuan
authors.forename: Rong-Hui
authors.initials: RH
authors.lastname: Li
authors.forename: Feng
authors.initials: F
authors.lastname: Bi
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D010957:Plasmids; D013274:Stomach Neoplasms; D014162:Transfection; D020741:rho GTP-Binding Proteins / Q000235:genetics*
chemical_list: C117386:DIRAS3 protein, human; D020741:rho GTP-Binding Proteins