title: Association of interleukin-1 beta (+3954) gene polymorphism and gingival crevicular fluid levels in patients with aggressive and chronic periodontitis.
abstract: OBJECTIVE
  The immune mechanisms and genetic variations that regulate genetic expression, production and biological activity of IL-1beta, are thought to play an important role in the pathogenesis of periodontal disease. The aims of the present study were to analyse interleukin (IL)-1beta (+3954) genotype and allele frequency in both chronic and aggressive periodontitis patients, and also to investigate whether this polymorphism is associated with gingival crevicular fluid (GCF) IL-1beta levels, periodontal disease severity and clinical parameters in subjects of Turkish origin.
  
  
  METHODS
  A total of 147 individuals were enrolled in the study including 56 aggressive periodontitis (AP), 44 chronic periodontitis (CP) patients and 47 healthy controls (C). Single nucleotide polymorphism at IL-1beta (+3954) is analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). GCF samples were analyzed for IL-1beta, using enzyme linked immunosorbent assay (ELISA).
  
  
  RESULTS
  The distribution of genotypes and allele frequencies for IL-1beta (+3954) were similar among the groups, in spite of a trend toward a higher frequency of allele 2 in the patient groups. The genotype distribution and allele frequencies were also not different after stratification of subjects according to the clinical attachment level (CAL < 4 mm and CAL > 4mm). No differences were found between the GCF IL-1beta levels of the different genotypes. Allele 2 was associated with increased bleeding on probing (BOP) sites in chronic periodontitis patients.
  
  
  CONCLUSIONS
  The results of this study do not support that genetic polymorphism in the IL-1beta (+3954) could be identified as a susceptibility or severity factor in aggressive periodontitis, in the present population. The association of allele 2 frequency and higher percentage of BOP sites in chronic periodontitis suggest that IL-1beta (+3954) potentially play a significant but not major role in the clinical outcome.
authors.affiliation: Department of Oral Pathology, Faculty of Dentistry, Gazi University, Ankara, Turkey. ozlemozertr@gmail.com
authors.forename: O Ozer
authors.initials: OO
authors.lastname: Yücel
authors.forename: E
authors.initials: E
authors.lastname: Berker
authors.forename: L
authors.initials: L
authors.lastname: Mescil
authors.forename: K
authors.initials: K
authors.lastname: Eratalay
authors.forename: E
authors.initials: E
authors.lastname: Tepe
authors.forename: I
authors.initials: I
authors.lastname: Tezcan
mesh_terms: D000293:Adolescent; D000328:Adult; D010520:Aggressive Periodontitis / Q000235:genetics* / Q000378:metabolism; D000704:Analysis of Variance; D055113:Chronic Periodontitis / Q000235:genetics* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D005883:Gingival Crevicular Fluid / Q000378:metabolism*; D006801:Humans; D053583:Interleukin-1beta / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D010512:Periodontal Index; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D012720:Severity of Illness Index; D014421:Turkey; D055815:Young Adult
chemical_list: D053583:Interleukin-1beta