title: Characterization of an acid-tolerant β-1,4-glucosidase from Fusarium oxysporum and its potential as an animal feed additive.
abstract: An extracellular β-glucosidase (BGL) from Fusarium oxysporum was purified to homogeneity by a single chromatography step on a gel filtration column. The optimum activity of BGL on cellobiose was observed at pH 5.0 and 60 °C. Under the same conditions, the K(m) and V(max) values for p-nitrophenyl β-D-glucopyranoside and cellobiose were 2.53 mM, 268 U mg protein(-1) and 20.3 mM, 193 U mg protein(-1), respectively. The F. oxysporum BGL enzyme was highly stable at acidic pH (t 1/2 = 470 min at pH 3). A commercial BGL Novo188 (Novozymes) and F. oxysporum BGL were compared in their ability to supplement Celluclast 1.5 L (Novozymes). In comparison with the commercial Novo188 (267 mg g substrate(-1)), F. oxysporum BGL supplementation released more reducing sugars (330 mg g substrate(-1)) from cellulose under simulated gastric conditions. These properties make F. oxysporum BGL a good candidate as a new commercial BGL to improve the nutrient bioavailability of animal feed.
authors.affiliation: Department of Chemical Engineering, Konkuk University, Seoul, 143-701, Republic of Korea.
authors.forename: Zongpei
authors.initials: Z
authors.lastname: Zhao
authors.forename: Priyadharsini
authors.initials: P
authors.lastname: Ramachandran
authors.forename: Tae-Su
authors.initials: TS
authors.lastname: Kim
authors.forename: Zhilei
authors.initials: Z
authors.lastname: Chen
authors.forename: Marimuthu
authors.initials: M
authors.lastname: Jeya
authors.forename: Jung-Kul
authors.initials: JK
authors.lastname: Lee
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D004795:Enzyme Stability; D005503:Food Additives / Q000737:chemistry* / Q000302:isolation & purification; D005670:Fusarium / Q000737:chemistry / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008970:Molecular Weight; D013379:Substrate Specificity; D013696:Temperature; D001617:beta-Glucosidase / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D005503:Food Additives; D001617:beta-Glucosidase