title: Engineered heterologous FPP synthases-mediated Z,E-FPP synthesis in E. coli.
abstract: Production of Z-type farnesyl diphosphate (FPP) has not been reported in Escherichia coli. Here we present the fusion enzyme (ILRv) of E. coli E,E-FPP synthase (IspA) and Mycobacterium tuberculosis Z,E-FPP synthase (Rv1086), which can produce primarily Z,E-FPP rather than E,E-FPP, the predominant stereoisomer found in most organisms. Z,E-farnesol (FOH) was produced from E. coli harboring the bottom portion of the MVA pathway and the fusion FPP synthase (ILRv) at a titer of 115.6 mg/L in 2YT medium containing 1% (v/v) glycerol as a carbon source and 5 mM mevalonate. The Z,E-FOH production was improved by 15-fold, compared with 7.7 mg/L obtained from the co-overexpression of separate IspA and Rv1086. The Z,E-FPP was not metabolized in native metabolic pathways of E. coli. It would be of interest to produce Z,E-FPP derived sesquiterpenes from recombinant E. coli due to no loss of Z,E-FPP substrate in endogenous metabolism of the host strain.
authors.affiliation: Division of Applied Life Science-BK21 Program, PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Chonglong
authors.initials: C
authors.lastname: Wang
authors.forename: Jia
authors.initials: J
authors.lastname: Zhou
authors.forename: Hui-Jeong
authors.initials: HJ
authors.lastname: Jang
authors.forename: Sang-Hwal
authors.initials: SH
authors.lastname: Yoon
authors.forename: Jae-Yean
authors.initials: JY
authors.lastname: Kim
authors.forename: Seung-Goo
authors.initials: SG
authors.lastname: Lee
authors.forename: Eui-Sung
authors.initials: ES
authors.lastname: Choi
authors.forename: Seon-Won
authors.initials: SW
authors.lastname: Kim
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D005204:Farnesol / Q000378:metabolism; D051228:Geranyltranstransferase / Q000096:biosynthesis* / Q000235:genetics; D008798:Mevalonic Acid / Q000378:metabolism; D009169:Mycobacterium tuberculosis / Q000201:enzymology* / Q000235:genetics; D011106:Polyisoprenyl Phosphates / Q000096:biosynthesis*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics; D012717:Sesquiterpenes
chemical_list: D001426:Bacterial Proteins; D011106:Polyisoprenyl Phosphates; D011993:Recombinant Fusion Proteins; D012717:Sesquiterpenes; D005204:Farnesol; C004808:farnesyl pyrophosphate; D051228:Geranyltranstransferase; D008798:Mevalonic Acid