title: Content of the Alternaria mycotoxin tenuazonic acid in food commodities determined by a stable isotope dilution assay.
abstract: The Alternaria mycotoxin tenuazonic acid (TA) was quantified in fruit juices (n = 50), cereals (n = 12) and spices (n = 38) using a recently developed stable isotope dilution assay (SIDA). [(13) C6,(15) N]-TA was used as the internal standard. Method validation revealed low limits of detection (LODs) of 0.15 μg/kg (fruit juices), 1.0 μg/kg (cereals) and 17 μg/kg (spices). The respective limits of quantitation were about three times higher. Recovery was about 100% for all matrices. The precision (relative standard deviation of replicate analyses of naturally contaminated samples) was 4.2% (grape juice; 1.7 μg/kg), 3.5% (whole wheat flour; 36 μg/kg) and 0.9% (curry powder; 215 μg/kg). The median content of TA in the analyzed samples was 1.8 μg/kg (fruit juices), 16 μg/kg (cereals) and 500 μg/kg (spices). Positive samples amounted to 86% (fruit juices), 92% (cereals) and 87% (spices).
authors.affiliation: Technische Universität München, Alte Akademie 10, 85354, Freising-Weihenstephan, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Asam
authors.forename: Martina
authors.initials: M
authors.lastname: Lichtenegger
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Michael
authors.initials: M
authors.lastname: Rychlik
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D007553:Isotope Labeling / Q000379:methods; D009183:Mycotoxins / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013720:Tenuazonic Acid / Q000032:analysis*
chemical_list: D009183:Mycotoxins; D013720:Tenuazonic Acid