title: Molecular cloning and characterization of two 12 kDa FK506-binding protein genes in the Chinese oak silkworm, Antheraea pernyi.
abstract: Two 12 kDa FK506-binding protein (FKBP12) genes were isolated and characterized from Chinese oak silkworm Antheraea pernyi , an important agricultural and edible insect, designated ApFKBP12 A and B, respectively. Both ApFKBP12 A and B contained 108 amino acids with 82% sequence identity. Phylogenetic analysis showed that FKBP12 B sequences of A. pernyi, Bombyx mori , and Danaus plexippus were clearly separated from FKBP12 A sequences of these three species, suggesting that insect FKBP12 A and B may have been evolving independently. RT-PCR analyses revealed that two ApFKBP12 genes were expressed during the four developmental stages and in all tested tissues, and that the mRNA expression level of the ApFKBP12 A gene was significantly higher than that of the ApFKBP12 B gene. After heat shock treatment, expressions of the two FKBP12 genes were up-regulated, but at different time points. The results suggested that each paralogue of the FKBP12 genes may play a distinct functional role in the development of A. pernyi.
authors.affiliation: Insect Resource Center for Engineering and Technology of Liaoning Province, College of Bioscience and Biotechnology, Shenyang Agricultural University, Liaoning, Shenyang 110866, China.
authors.forename: Mo
authors.initials: M
authors.lastname: Chen
authors.forename: Miao-Miao
authors.initials: MM
authors.lastname: Chen
authors.forename: Rui
authors.initials: R
authors.lastname: Yao
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Huan
authors.initials: H
authors.lastname: Wang
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Li
authors.forename: Yan-Qun
authors.initials: YQ
authors.lastname: Liu
mesh_terms: D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D015723:Gene Library; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D022061:Tacrolimus Binding Protein 1A / Q000235:genetics* / Q000378:metabolism
chemical_list: D019476:Insect Proteins; D022061:Tacrolimus Binding Protein 1A