title: [Construction of Corynebacterium crenatum AS 1.542 δ argR and analysis of transcriptional levels of the related genes of arginine biosynthetic pathway].
abstract: OBJECTIVE
  ArgR, coded by the argR gene from Corynebacterium crenatum AS 1.542, acts as a negative regulator in arginine biosynthetic pathway. However, the effect of argR on transcriptional levels of the related biosynthetic genes has not been reported. Here, we constructed a deletion mutant of argR gene: C. crenatum AS 1.542 Delta argR using marker-less knockout technology, and compared the changes of transcriptional levels of the arginine biosynthetic genes between the mutant strain and the wild-type strain.
  
  
  METHODS
  We used marker-less knockout technology to construct C. crenatum AS 1.542 Delta argR and analyzed the changes of the relate genes at the transcriptional level using real-time fluorescence quantitative PCR.
  
  
  RESULTS
  C. crenatum AS 1.542 Delta argR was successfully obtained and the transcriptional level of arginine biosynthetic genes in this mutant increased significantly with an average of about 162.1 folds.
  
  
  CONCLUSIONS
  The arginine biosynthetic genes in C. crenatum are clearly controlled by the negative regulator ArgR. However, the deletion of this regulator does not result in a clear change in arginine production in the bacteria.
authors.affiliation: Key Laboratory of Functional Small organic molecule, Ministry of Education, College of Life Science, Jiangxi Normal University, Nanchang 330022, China. xuelanchen14@yahoo.com.cn
authors.forename: Xuelan
authors.initials: X
authors.lastname: Chen
authors.forename: Li
authors.initials: L
authors.lastname: Tang
authors.forename: Haitao
authors.initials: H
authors.lastname: Jiao
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Xiong
mesh_terms: D001120:Arginine / Q000096:biosynthesis*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D053898:Biosynthetic Pathways; D003352:Corynebacterium / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D012097:Repressor Proteins; D001120:Arginine