title: Expression of recombinant glycoproteins in mammalian cells: towards an integrative approach to structural biology.
abstract: Mammalian cells are rapidly becoming the system of choice for the production of recombinant glycoproteins for structural biology applications. Their use has enabled the structural investigation of a whole new set of targets including large, multi-domain and highly glycosylated eukaryotic cell surface receptors and their supra-molecular assemblies. We summarize the technical advances that have been made in mammalian expression technology and highlight some of the structural insights that have been obtained using these methods. Looking forward, it is clear that mammalian cell expression will provide exciting and unique opportunities for an integrative approach to the structural study of proteins, especially of human origin and medically relevant, by bridging the gap between the purified state and the cellular context.
authors.affiliation: Division of Structural Biology, University of Oxford, Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford OX3 7BN, UK. radu@strubi.ox.ac.uk
authors.forename: A Radu
authors.initials: AR
authors.lastname: Aricescu
authors.forename: Raymond J
authors.initials: RJ
authors.lastname: Owens
mesh_terms: D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D003412:Cricetulus; D015870:Gene Expression; D006023:Glycoproteins / Q000096:biosynthesis* / Q000737:chemistry*; D006031:Glycosylation; D057809:HEK293 Cells; D006801:Humans; D008322:Mammals; D008967:Molecular Biology; D011487:Protein Conformation; D011956:Receptors, Cell Surface / Q000096:biosynthesis / Q000737:chemistry; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000737:chemistry*; D015398:Signal Transduction; D013194:Staining and Labeling
chemical_list: D006023:Glycoproteins; D011956:Receptors, Cell Surface; D011994:Recombinant Proteins