title: Micropatterning of cells on electron-irradiated poly(dimethylsiloxane) surface.
abstract: In this study, a facile route to fabricate micropatterns of cells is presented on the basis of electron irradiation of poly(dimethylsiloxane) (PDMS). PDMS films were irradiated with electron beams through a pattern mask with micrometer-sized grids. After irradiation, the changes in the chemical composition, morphology, and wettability of the PDMS surface were investigated by using an X-ray photoelectron spectrometer, an atomic force microscope, and a contact anglometer. The results of the surface analysis revealed that the hydrophobic PDMS surface was changed into a hydrophilic one by the electron irradiation. Furthermore, on the basis of cell culturing on the selectively-irradiated PDMS, cells such as NIH3T3 and L929 were selectively adhered to and proliferated on the irradiated regions of the PDMS surface, resulting in the micropatterns of the cells on the PDMS surface.
authors.affiliation: Advanced Radiation Technology Institute, 1266 Sinjeong-dong, Jeongeup-si, Jeollabuk-do 580-185, Republic of Korea.
authors.forename: Eun Je
authors.initials: EJ
authors.lastname: Lee
authors.forename: In-Tae
authors.initials: IT
authors.lastname: Hwang
authors.forename: Chan-Hee
authors.initials: CH
authors.lastname: Jung
authors.forename: Ho-Je
authors.initials: HJ
authors.lastname: Kwon
authors.forename: Jae-Hak
authors.initials: JH
authors.lastname: Choi
authors.forename: Min Goo
authors.initials: MG
authors.lastname: Hur
authors.forename: Sung Oh
authors.initials: SO
authors.lastname: Cho
authors.forename: Kwanwoo
authors.initials: K
authors.lastname: Shin
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D004129:Dimethylpolysiloxanes / Q000494:pharmacology*; D004583:Electrons; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects*; D051379:Mice; D018625:Microscopy, Atomic Force; D055616:Microtechnology / Q000379:methods*; D041681:NIH 3T3 Cells; D056951:Photoelectron Spectroscopy; D013499:Surface Properties; D014867:Water / Q000737:chemistry
chemical_list: D004129:Dimethylpolysiloxanes; D014867:Water