title: Proteomic analysis of interstitial aortic valve cells acquiring a pro-calcific profile.
abstract: Cell-driven processes are now considered of relevance for the pathogenesis of aortic stenosis. In particular, during calcific valve degeneration, interstitial valve cells (VIC) resident in the leaflet can acquire an osteogenic/pro-calcific profile and actively contribute to matrix mineralization. The proteomic study described in this chapter is undertaken to investigate modifications in the proteome of bovine aortic VIC acquiring a calcifying phenotype. This approach can be useful to clarify cellular pathways involved in VIC pro-calcific differentiation and identify innovative therapeutic targets.
authors.affiliation: Department of Medicine, Padova University, Padova, Italy.
authors.forename: Millioni
authors.initials: M
authors.lastname: Renato
authors.forename: Elisa
authors.initials: E
authors.lastname: Bertacco
authors.forename: Cinzia
authors.initials: C
authors.lastname: Franchin
authors.forename: Giorgio
authors.initials: G
authors.lastname: Arrigoni
authors.forename: Marcello
authors.initials: M
authors.lastname: Rattazzi
mesh_terms: D000818:Animals; D001021:Aortic Valve / Q000378:metabolism* / Q000473:pathology; D001024:Aortic Valve Stenosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002114:Calcinosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002417:Cattle; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D015870:Gene Expression; D032342:Muscle Cells / Q000378:metabolism* / Q000473:pathology; D010449:Peptide Mapping; D010641:Phenotype; D040901:Proteomics; D015398:Signal Transduction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization