title: The expression and significance of neuropilin-1 (NRP-1) on glioma cell lines and glioma tissues.
abstract: We detected the expressions of neuropilin-1 (NRP-1) on glioma cell lines and glioma tissues by neuropilin-1 monoclonal antibody (NRP-1 MAb). The expressions of NRP-1 on glioma cell lines and glioma tissues were detected by Western blot, Immunofluorescence staining and Immunocytochemistry staining, which indicated that the NRP-1 was over-expressed on glioma cell lines (U251, U87 and C6) and glioma tissues. NRP-1 MAb prepared successfully could combine specifically to membranes of glioma cell lines (U251, U87, and C6). It lays a foundation for further research on biological treatment of glioma.
authors.affiliation: Department of Neurosurgery, Zhongda Hospital Southeast University, Nanjing, 210009, China. neuro_clk@hotmail.com
authors.forename: Lukui
authors.initials: L
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Miao
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Tang
authors.forename: Haipeng
authors.initials: H
authors.lastname: Zhang
authors.forename: Shengyu
authors.initials: S
authors.lastname: Wang
authors.forename: Fanghong
authors.initials: F
authors.lastname: Luo
authors.forename: Jianghua
authors.initials: J
authors.lastname: Yan
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D005455:Fluorescent Antibody Technique; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D060787:Neoplasm Grading; D039942:Neuropilin-1 / Q000378:metabolism*; D051381:Rats
chemical_list: D039942:Neuropilin-1