title: Detection of specific antibodies against tembusu virus in ducks by use of an E protein-based enzyme-linked immunosorbent assay.
abstract: We developed an enzyme-linked immunosorbent assay (ELISA) using eukaryotically expressed E protein as the antigen (termed E-ELISA) to detect antibodies to tembusu virus (TMUV) in ducks. The E-ELISA did not react with antisera to other known pathogens, indicating the E protein is specific for recognizing anti-TMUV antibodies. Compared to the serum neutralization test, the specificity and sensitivity of the E-ELISA was 93.2 and 97.8%, respectively. Therefore, this E-ELISA is a sensitive and rapid method for detecting antibodies against TMUV in ducks.
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin, China.
authors.forename: Xiuchen
authors.initials: X
authors.lastname: Yin
authors.forename: Rang
authors.initials: R
authors.lastname: Lv
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Chen
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
authors.forename: Ronghong
authors.initials: R
authors.lastname: Hua
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral; D004372:Ducks; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005416:Flavivirus / Q000276:immunology*; D018177:Flavivirus Infections / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D012680:Sensitivity and Specificity; D014730:Veterinary Medicine / Q000379:methods*; D014759:Viral Envelope Proteins; D014773:Virology / Q000379:methods*
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D014759:Viral Envelope Proteins; C065696:glycoprotein E, Flavivirus