title: Crystal structure of the guanylate kinase domain from discs large homolog 1 (DLG1/SAP97).
abstract: Discs large homolog 1 (DLG1/SAP97) is involved in the development and regulation of neuronal and immunological synapses. DLG1 is a member of the membrane associated guanylate kinase (MAGUK) family of proteins, which function as molecular scaffolds. The C-terminal guanylate kinase (GK) domain of DLG1 binds peptides with a phosphorylated serine residue. In this study, we solved the crystal structure of the GK domain of human DLG1. The C-terminal tail of DLG1 is bound to the peptide-binding site of an adjacent symmetry-related DLG1 GK molecule. The binding direction of the C-terminal tail to the peptide-binding site is opposite to that of the phosphorylated LGN peptide in complex with the rat DLG1 GK domain. The C-terminal tail forms a 310 helix, which is also different from the conformation of the phosphorylated LGN peptide. Nevertheless, the side chain interactions of the C-terminal tail with the DLG1 GK domain are similar to those of the phosphorylated LGN peptide.
authors.affiliation: Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, Tokyo 113-0033, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Mori
authors.forename: Yuta
authors.initials: Y
authors.lastname: Tezuka
authors.forename: Akihiko
authors.initials: A
authors.lastname: Arakawa
authors.forename: Noriko
authors.initials: N
authors.lastname: Handa
authors.forename: Mikako
authors.initials: M
authors.lastname: Shirouzu
authors.forename: Tetsu
authors.initials: T
authors.lastname: Akiyama
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Yokoyama
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry*; D000818:Animals; D001665:Binding Sites; D018360:Crystallography, X-Ray; D000074288:Discs Large Homolog 1 Protein; D051528:Guanylate Kinases / Q000737:chemistry; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry*; D008958:Models, Molecular; D011487:Protein Conformation; D054730:Protein Interaction Domains and Motifs; D051381:Rats; D011994:Recombinant Proteins / Q000737:chemistry; D013045:Species Specificity
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C513314:DLG1 protein, human; D000074288:Discs Large Homolog 1 Protein; C501119:Dlg1 protein, rat; D008565:Membrane Proteins; D011994:Recombinant Proteins; D051528:Guanylate Kinases