title: A multicomponent method for Fusarium toxins in cereal based food and feed samples using HPLC-MS/MS.
abstract: A reliable, sensitive and selective multicomponent method has been developed to determine 12 differentFusarium mycotoxins (trichothecenes type A and B, zearalenone) simultaneously in cereal and grain samples using liquid chromatography tandem mass spectrometry (LC-ESI-MS/MS). The sample preparation based on a standard extraction step followed by two different kinds of solid phase clean-up (multifunctional MycoSep(®) material) for trichothecenes, and an immuno-affinity purification which combined antibodies for aflatoxins, ochratoxin A and zearalenone (AOZ-IAC). For quantification of zearalenone (ZON) an internal standard (zearalanone, ZAN) was used, whereas for trichothecenes a recovery standard (verrucarol, VOL) was applied. The average recoveries for the trichothecenes ranged from 65% for nivalenol (NIV) up to 96% for deoxynivalenol (DON) and 89% for zearalenone. The limit of quantification is different for each of the individual trichothecenes and in the range of 1 ppb to 10 ppb.
authors.affiliation: Eurofins/Wiertz-Eggert-Jörissen, Stenzelring 14b, D-21107, Hamburg, scarlett.biselli@wej de.
authors.forename: S
authors.initials: S
authors.lastname: Biselli
authors.forename: H
authors.initials: H
authors.lastname: Wegner
authors.forename: C
authors.initials: C
authors.lastname: Hummert