title: Motility and fertility of boar semen after liquid preservation at 5°C for more than 2 weeks.
abstract: This study investigated the effects of skim milk on the quality and fertility of boar spermatozoa under long-term chilled preservation. Semen samples were stored in Modena solution supplemented with 0 (control) to 50 mg/mL skim milk at 5°C for 4 weeks; spermatozoa stored with 7.5 and 15 mg/mL of skim milk (7.5-SM and 15-SM groups, respectively) exhibited significantly higher motility indices than those of the control group up to 3 weeks (P < 0.05), and the 7.5-SM group showed improved motility indices even after 4 weeks (P < 0.05). In vitro fertilization using spermatozoa in the 7.5-SM and 15-SM groups stored at 5°C for 2 weeks showed significantly higher fertilization rates of spermatozoa and the development rates to blastocyst than the control group (P < 0.05), and the 7.5-SM group showed similar rates of fertilization and blastocyst formation in the fresh non-stored spermatozoa group. After artificial insemination using spermatozoa stored for 2 weeks in the 7.5-SM group, healthy piglets were obtained. Boar spermatozoa can be stored at 5°C in a Modena solution containing skim milk. Supplementation of 7.5 mg/mL skim milk improves boar spermatozoa motility and fertility even after liquid preservation at 5°C for 2 weeks.
authors.affiliation: The United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Namula
authors.forename: Yoko
authors.initials: Y
authors.lastname: Sato
authors.forename: Risa
authors.initials: R
authors.lastname: Kodama
authors.forename: Kouta
authors.initials: K
authors.lastname: Morinaga
authors.forename: Vien Viet
authors.initials: VV
authors.lastname: Luu
authors.forename: Masayasu
authors.initials: M
authors.lastname: Taniguchi
authors.forename: Michiko
authors.initials: M
authors.lastname: Nakai
authors.forename: Fuminori
authors.initials: F
authors.lastname: Tanihara
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kikuchi
authors.forename: Takashi
authors.initials: T
authors.lastname: Nagai
authors.forename: Takeshige
authors.initials: T
authors.lastname: Otoi
mesh_terms: D000818:Animals; D003080:Cold Temperature; D005307:Fertilization in Vitro / Q000662:veterinary; D008297:Male; D012661:Semen / Q000502:physiology*; D055101:Semen Analysis; D012662:Semen Preservation / Q000379:methods*; D013081:Sperm Motility; D013552:Swine / Q000502:physiology*; D013997:Time Factors
keywords: AI; boar semen; chilled preservation; skim milk; sperm quality