title: Pooled human platelet lysate versus fetal bovine serum-investigating the proliferation rate, chromosome stability and angiogenic potential of human adipose tissue-derived stem cells intended for clinical use.
abstract: OBJECTIVE
  Because of an increasing focus on the use of adipose-derived stem cells (ASCs) in clinical trials, the culture conditions for these cells are being optimized. We compared the proliferation rates and chromosomal stability of ASCs that had been cultured in Dulbecco's modified Eagle's Medium (DMEM) supplemented with either pooled human platelet lysate (pHPL) or clinical-grade fetal bovine serum (FBS) (DMEM(pHPL) versus DMEM(FBS)).
  
  
  METHODS
  ASCs from four healthy donors were cultured in either DMEM(pHPL) or DMEM(FBS), and the population doubling time (PDT) was calculated. ASCs from two of the donors were expanded in DMEM(pHPL) or DMEM(FBS) and cultured for the final week before harvesting with or without the addition of vascular endothelial growth factor. We assessed the chromosomal stability (through the use of array comparative genomic hybridization), the expression of ASC and endothelial surface markers and the differentiation and angiogenic potential of these cells.
  
  
  RESULTS
  The ASCs that were cultured in pHPL exhibited a significantly shorter PDT of 29.6 h (95% confidence interval, 22.3-41.9 h) compared with those cultured in FBS, for which the PDT was 123.9 h (95% confidence interval, 95.6-176.2 h). Comparative genomic hybridization analyses revealed no chromosomal aberrations. Cell differentiation, capillary structure formation and cell-surface marker expression were generally unaffected by the type of medium supplement that was used or by the addition of vascular endothelial growth factor.
  
  
  CONCLUSIONS
  We observed that the use of pHPL as a growth supplement for ASCs facilitated a significantly higher proliferation rate compared with FBS without compromising genomic stability or differentiation capacity.
authors.affiliation: Department of Plastic Surgery, Breast Surgery & Burns 2102, Copenhagen University Hospital Rigshospitalet, Copenhagen, Denmark. stigfrederik@gmail.com
authors.forename: Stig-Frederik
authors.initials: SF
authors.lastname: Trojahn Kølle
authors.forename: Roberto S
authors.initials: RS
authors.lastname: Oliveri
authors.forename: Peter V
authors.initials: PV
authors.lastname: Glovinski
authors.forename: Maria
authors.initials: M
authors.lastname: Kirchhoff
authors.forename: Anders Bruun
authors.initials: AB
authors.lastname: Mathiasen
authors.forename: Jens Jørgen
authors.initials: JJ
authors.lastname: Elberg
authors.forename: Peter Stemann
authors.initials: PS
authors.lastname: Andersen
authors.forename: Krzysztof Tadeusz
authors.initials: KT
authors.lastname: Drzewiecki
authors.forename: Anne
authors.initials: A
authors.lastname: Fischer-Nielsen
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D001792:Blood Platelets / Q000378:metabolism*; D002417:Cattle; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D043171:Chromosomal Instability / Q000235:genetics*; D005260:Female; D006801:Humans; D018919:Neovascularization, Physiologic / Q000502:physiology*; D044967:Serum / Q000378:metabolism*; D013234:Stem Cells / Q000378:metabolism / Q000502:physiology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A
keywords: adipose tissue–derived stem cells; chromosomal stability; fetal bovine serum; mesenchymal stromal cells; pooled human platelet lysate