title: Ameloblasts serum-free conditioned medium: bone morphogenic protein 4-induced odontogenic differentiation of mouse induced pluripotent stem cells.
abstract: Induced pluripotent stem (iPS) cells possess the ability of self-renewal and can differentiate into cells of the three germ layers, both in vitro and in vivo. Here we report a new method to efficiently induce differentiation of mouse iPS cells into the odontogenic lineage. Using ameloblasts serum-free conditioned medium (ASF-CM), we successfully generated ameloblast-like cells from mouse iPS cells. Importantly, culturing mouse iPS cells in ASF-CM supplemented with BMP4 (ASF-BMP4) promoted odontogenic differentiation, which was evident by the upregulation of ameloblast-specific as well as odontoblast-specific genes. On the other hand, culturing mouse iPS cells in ASF-CM supplemented with noggin (ASF-noggin), an inhibitor of BMP4, abrogated this effect. These results suggest that mouse iPS cells can be induced by ASF-BMP4 to differentiate into ameloblast-like and odontoblast-like cells. The results of our study raise the possibility of using patient-specific iPS cells for tooth regeneration in the future. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Department of Stomatology, General Hospital of Beijing Military Command, Beijing, 100700, People's Republic of China.|Research and Development Centre for Tissue Engineering, School of Stomatology, Fourth Military Medical University, Xi'an, 710032, Shaanxi, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Stomatology, The 464 Hospital of Beijing PLA, Tianjin, 300191, People's Republic of China.
authors.forename: Ying-Feng
authors.initials: YF
authors.lastname: Liu
authors.affiliation: Research and Development Centre for Tissue Engineering, School of Stomatology, Fourth Military Medical University, Xi'an, 710032, Shaanxi, People's Republic of China.|Department of Orthodontics, School of Stomatology, Fourth Military Medical University, Xi'an, 710032, Shaanxi, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Orthodontics, School of Stomatology, Fourth Military Medical University, Xi'an, 710032, Shaanxi, People's Republic of China.
authors.forename: Yin-Zhong
authors.initials: YZ
authors.lastname: Duan
authors.affiliation: Research and Development Centre for Tissue Engineering, School of Stomatology, Fourth Military Medical University, Xi'an, 710032, Shaanxi, People's Republic of China.|Department of Oral Histology and Pathology, School of Stomatology, Fourth Military Medical University, Xi'an, 710032, Shaanxi, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
mesh_terms: D000565:Ameloblasts / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D055415:Bone Morphogenetic Protein 4 / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D017077:Culture Media, Conditioned / Q000494:pharmacology; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D009805:Odontogenesis / Q000187:drug effects*
chemical_list: C525810:Bmp4 protein, mouse; D055415:Bone Morphogenetic Protein 4; D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free
keywords: ameloblasts serum-free conditioned medium; bone morphogenic protein 4; microenviroment; mouse induced pluripotent stem cells; odontogenic lineage; tooth regeneration