title: Excystment-dependent alteration of protein expression in terrestrial ciliate Colpoda cucullus.
abstract: Protein expression during the excystment of Colpoda cucullus was studied by SDS-PAGE. The expression levels of 60-, 50- and 49-kDa proteins were markedly changed from the early to later stage of excystment. The 60-kDa protein (p60) was temporarily expressed first, and its expression was inhibited by actinomycin D. LC-MS/MS analysis showed that the amino acid sequences of p60 partially coincided with those of the Paramecium tetraurelia unnamed protein homologous to DEAD-box RNA helicase. These results suggest that p60 expression is enhanced by transcriptional regulation and may be involved in initiating the molecular events leading to cellular morphogenesis.
authors.affiliation: Department of Biological Science, Faculty of Science, Kochi University.
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Sogame
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Kojima
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Takeshita
authors.forename: Eiji
authors.initials: E
authors.lastname: Kinoshita
authors.forename: Ryoji
authors.initials: R
authors.lastname: Funadani
authors.forename: Tatsuomi
authors.initials: T
authors.lastname: Matsuoka
mesh_terms: D000595:Amino Acid Sequence; D016798:Ciliophora / Q000235:genetics* / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D008970:Molecular Weight; D015800:Protozoan Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D012987:Soil / Q000469:parasitology
chemical_list: D015800:Protozoan Proteins; D012987:Soil