title: Spray dried chitosan-EDTA superior microparticles as solid substrate for the oral delivery of amphotericin B.
abstract: The present investigation was aimed at synthesis of chitosan-EDTA superior microparticles (COECH) bearing high oil adsorbing and oil desorbing properties. These superior particles were prepared by thermal amide conjugation of COO(-) group of EDTA with NH2 group of chitosan employing spray-drying technique. The synthesis was optimized using 4(2) full factorial design. The particles showed high oil adsorbing capacity as well as oil desorbing capacity with enhanced dispersive components of surface free energy as compared to Aerosil 200. In addition, these COECH microparticles showed higher amphotericin B loading capacity, enhancement in the in vitro dissolution performance (12-fold) and produces nanoemulsion in the size range of 70-90 nm. Further, the results were in consonance with those observed during ex vivo performance. Thus, the findings revealed simple synthesis of COECH microparticles that showed superior properties of solid substrate for the development of amphotericin B nanoemulsion.
authors.affiliation: Pharmaceutics Division, Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala 147002, India.
authors.forename: Kuldeep
authors.initials: K
authors.lastname: Singh
authors.forename: A K
authors.initials: AK
authors.lastname: Tiwary
authors.forename: Vikas
authors.initials: V
authors.lastname: Rana
mesh_terms: D000284:Administration, Oral; D000666:Amphotericin B / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D000935:Antifungal Agents / Q000737:chemistry* / Q000378:metabolism; D000981:Antiprotozoal Agents / Q000737:chemistry* / Q000378:metabolism; D002152:Calorimetry, Differential Scanning; D048271:Chitosan / Q000138:chemical synthesis* / Q000737:chemistry; D003890:Desiccation; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism; D004339:Drug Compounding; D004492:Edetic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D004655:Emulsions; D066298:In Vitro Techniques; D007408:Intestinal Absorption; D007421:Intestine, Small / Q000378:metabolism; D008863:Microspheres; D034421:Sus scrofa; D013816:Thermodynamics
chemical_list: D000935:Antifungal Agents; D000981:Antiprotozoal Agents; D004337:Drug Carriers; D004655:Emulsions; C114304:chitosan-EDTA; D000666:Amphotericin B; D048271:Chitosan; D004492:Edetic Acid