title: Evaluation of various electrode materials for detection of oxidized low-density lipoproteins.
abstract: Measurement of oxidized low-density lipoprotein (LDL) generated by oxidative stress of various kinds might be useful for evaluating the risk of cardiovascular disease. We evaluated some electrode materials to detect oxidized LDL electrochemically. Some carbon nanotube dispersions were studied as electrode materials. Native LDL was isolated from normal human serum using ultracentrifugation. Oxidized LDL was prepared by treating the native LDL with CuSO4. Electrodes were fabricated by depositing the nanotube dispersion on a gold electrode, with subsequent drying. The potential change of the electrode against a reference electrode was monitored before and after adding native LDL or oxidized LDL. Only acid-treated carbon nanotubes were able to discriminate both LDL preparations, perhaps because of the carboxylic acid groups introduced on the nanotube by acid treatment.
authors.affiliation: Faculty of Health Sciences, Hokkaido University, Sapporo, Hokkaido, 060-0812, Japan.
authors.forename: Seiji
authors.initials: S
authors.lastname: Takeda
authors.forename: Shu-Ping
authors.initials: SP
authors.lastname: Hui
authors.forename: Hirotoshi
authors.initials: H
authors.lastname: Fuda
authors.forename: Shigeki
authors.initials: S
authors.lastname: Jin
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Sakurai
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ishii
authors.forename: Koichi
authors.initials: K
authors.lastname: Mukasa
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Sueoka
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Chiba
mesh_terms: D000328:Adult; D017607:Aluminum Compounds / Q000737:chemistry; D004566:Electrodes; D006801:Humans; D008077:Lipoproteins, LDL / Q000032:analysis*; D018020:Lithium Compounds / Q000737:chemistry; D008422:Materials Testing; D037742:Nanotubes, Carbon / Q000737:chemistry; D010084:Oxidation-Reduction
chemical_list: D017607:Aluminum Compounds; D008077:Lipoproteins, LDL; D018020:Lithium Compounds; D037742:Nanotubes, Carbon; C099252:oxidized low density lipoprotein; C042073:lithium aluminum hydride