title: In vitro comparison of induction capacity and biomineralization ability of mineral trioxide aggregate and a bioceramic root canal sealer.
abstract: OBJECTIVE
  To compare the effect of mineral trioxide aggregate (MTA) and iRoot SP, a bioceramic root canal sealer, on the cell viability, hard tissue deposition capacity and odontogenic differentiation of human tooth germ stem cells (hTGSCs).
  
  
  METHODS
  The dental materials MTA, iRoot SP and Dycal were packed into Teflon rings and placed on transwell inserts for toxicity evaluations by the MTS assay on days 3 and 7. Dycal was used as a positive control for the cell viability assay. Teflon rings were cocultured with hTGSCs, followed by the induction of odontogenic differentiation. The odontogenic differentiation of hTGSCs and biomineralization ability of the materials were evaluated by analysing the mRNA expression levels of dentine sialophosphoprotein (DSPP) and collagen type 1A (COL1A) by real-time polymerase chain reaction expression analysis, measurement of alkaline phosphatase (ALP) activity and visualization of calcium deposits by von Kossa staining.
  
  
  RESULTS
  MTA and iRoot SP exhibited no cytotoxicity, but Dycal caused cytotoxicity (P < 0.05) of almost all of the cells after 7 days. MTA significantly stimulated (P < 0.05) the odontogenic differentiation of hTGSCs compared with iRoot SP. MTA and iRoot SP increased (P < 0.05) the mRNA levels of COL1A and DSPP mRNA compared with noninduced hTGSCs, which served as a negative control (NC). iRoot SP, however, significantly decreased (P < 0.05) COL1A and DSPP mRNA expression levels compared with the PC.
  
  
  CONCLUSIONS
  MTA and iRoot SP induced hTGSC differentiation into odontoblast-like cells, but MTA might provide more inductive potential and hard tissue deposition compared with iRoot SP.
authors.affiliation: Department of Endodontics, Faculty of Dentistry, Yeditepe University, Istanbul, Turkey.
authors.forename: E P
authors.initials: EP
authors.lastname: Güven
authors.forename: P N
authors.initials: PN
authors.lastname: Taşlı
authors.forename: M E
authors.initials: ME
authors.lastname: Yalvac
authors.forename: N
authors.initials: N
authors.lastname: Sofiev
authors.forename: M B
authors.initials: MB
authors.lastname: Kayahan
authors.forename: F
authors.initials: F
authors.lastname: Sahin
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D017607:Aluminum Compounds; D001672:Biocompatible Materials; D002113:Calcification, Physiologic; D017610:Calcium Compounds; D002470:Cell Survival; D002516:Ceramics; D004338:Drug Combinations; D006801:Humans; D066298:In Vitro Techniques; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates
chemical_list: D017607:Aluminum Compounds; D001672:Biocompatible Materials; D017610:Calcium Compounds; D004338:Drug Combinations; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate; D000469:Alkaline Phosphatase
keywords: calcium salt deposition; calcium silicate cements; human tooth germ stem cells (hTGSCs); iRoot SP; mineral trioxide aggregate (MTA); odontogenic differentiation