title: Assessment of genotoxic effects of flumorph by the comet assay in mice organs.
abstract: The present study investigated the genotoxic effects of flumorph in various organs (brain, liver, spleen, kidney and sperm) of mice. The DNA damage, measured as comet tail length (µm), was determined using the alkaline comet assay. The comet assay is a sensitive assay for the detection of genotoxicity caused by flumorph using mice as a model. Statistically significant increases in comet assay for both dose-dependent and duration-dependent DNA damage were observed in all the organs assessed. The organs exhibited the maximum DNA damage in 96 h at 54 mg/kg body weight. Brain showed maximum DNA damage followed by spleen > kidney > liver > sperm. Our data demonstrated that flumorph had induced systemic genotoxicity in mammals as it caused DNA damage in all tested vital organs, especially in brain and spleen.
authors.affiliation: 1Key Laboratory of Pesticide Chemistry and Application, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: T
authors.initials: T
authors.lastname: Zhang
authors.forename: Q
authors.initials: Q
authors.lastname: Zhao
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: Ning
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002452:Cell Count; D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D005659:Fungicides, Industrial / Q000633:toxicity*; D008297:Male; D051379:Mice; D009025:Morpholines / Q000633:toxicity*; D009153:Mutagens
chemical_list: D005659:Fungicides, Industrial; D009025:Morpholines; D009153:Mutagens; C528737:flumorph
keywords: DNA damage; alkaline comet assay; flumorph; genotoxicity