title: Production and characterization of an extracellular polysaccharide from Streptomyces violaceus MM72.
abstract: The isolation, optimization, purification and characterization of an extracellular polysaccharide (EPS) from a marine actinobacterium, Streptomyces violaceus MM72 were investigated. Medium composition and culture conditions for the EPS production by S. violaceus MM72 were optimized using two statistical methods: Plackett-Burman design applied to find the key ingredients and conditions for the best yield of EPS production and central composite design used to optimize the concentration of the three significant variables: glucose, tryptone and NaCl. The preferable culture conditions for EPS production were pH 7.0, temperature 35°C and NaCl concentration 2.0% for 120h with fructose and yeast extract as best carbon and nitrogen sources, respectively. The results showed that S. violaceus MM72 produced a kind of EPS having molecular weight of 8.96×10(5)Da. In addition, the EPS showed strong DPPH radical-scavenging activity, superoxide scavenging and metal chelating activities while moderate inhibition of lipid peroxidation and reducing activities determined in this study. These results showed the great potential of EPS produced by S. violaceus MM72 could be used in industry in place of synthetic compounds. The EPS from S. violaceus MM72 may be a new source of natural antioxidants with potential value for health, food and therapeutics.
authors.affiliation: Marine Biotechnology Laboratory, Department of Chemistry and Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, Republic of Korea. manimaribtech@gmail.com
authors.forename: Panchanathan
authors.initials: P
authors.lastname: Manivasagan
authors.forename: Palaniappan
authors.initials: P
authors.lastname: Sivasankar
authors.forename: Jayachandran
authors.initials: J
authors.lastname: Venkatesan
authors.forename: Kalimuthu
authors.initials: K
authors.lastname: Senthilkumar
authors.forename: Kannan
authors.initials: K
authors.lastname: Sivakumar
authors.forename: Se-Kwon
authors.initials: SK
authors.lastname: Kim
mesh_terms: D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors; D003470:Culture Media; D005163:Factor Analysis, Statistical; D005285:Fermentation; D016166:Free Radical Scavengers / Q000302:isolation & purification* / Q000494:pharmacology; D005632:Fructose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D015227:Lipid Peroxidation / Q000187:drug effects; D008970:Molecular Weight; D010461:Peptones / Q000378:metabolism; D010851:Picrates / Q000037:antagonists & inhibitors; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification* / Q000494:pharmacology; D012965:Sodium Chloride / Q000378:metabolism; D013302:Streptomyces / Q000737:chemistry / Q000378:metabolism*; D013481:Superoxides / Q000037:antagonists & inhibitors; D013696:Temperature
chemical_list: D001713:Biphenyl Compounds; D003470:Culture Media; D016166:Free Radical Scavengers; D010461:Peptones; D010851:Picrates; D011135:Polysaccharides, Bacterial; D013481:Superoxides; D005632:Fructose; D012965:Sodium Chloride; C001830:tryptones; C004931:1,1-diphenyl-2-picrylhydrazyl; D005947:Glucose