title: Downregulated expression of hepatoma-derived growth factor (HDGF) reduces gallbladder cancer cell proliferation and invasion.
abstract: Hepatoma-derived growth factor (HDGF), a heparin-binding growth factor, has a wide range of biological functions, including mitogenic activity and vascular development. Recent studies demonstrated that HDGF also acted as an oncogene with aberrantly increased activity in multiple human cancers; however, little is known about the biological function of HDGF in gallbladder cancer (GBC). In this study, we focused on the clinical significance and biological functions of HDGF in GBC and found that Nuclear HDGF protein overexpression was frequently detected in GBC tissues. Patients with nuclear HDGF-positive tumors had worse overall survival than patients with HDGF-negative tumors. Furthermore, treatment of GBC lines with HDGF-targeting siRNA oligonucleotides (HDGF-siRNA) significantly reduced the proliferation of GBC-SD and SGC-996 cell lines and diminished both anchorage-independent growth on soft agar and cell migration. These data indicate that HDGF acts as a putative oncogene in GBC and could be a novel diagnostic and therapeutic target for GBC.
authors.affiliation: Department of General Surgery, Xinhua hospital, School of Medicine, Shanghai Jiaotong University, Room 615, NO.8 Building, 1665 kongjiang road, Shanghai 200092, People's Republic of China.
authors.forename: Maolan
authors.initials: M
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Shen
authors.forename: Xiangsong
authors.initials: X
authors.lastname: Wu
authors.forename: Bingtai
authors.initials: B
authors.lastname: Zhang
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.forename: Hao
authors.initials: H
authors.lastname: Weng
authors.forename: Qian
authors.initials: Q
authors.lastname: Ding
authors.forename: Zhujun
authors.initials: Z
authors.lastname: Tan
authors.forename: Guofeng
authors.initials: G
authors.lastname: Gao
authors.forename: Jiasheng
authors.initials: J
authors.lastname: Mu
authors.forename: Jiahua
authors.initials: J
authors.lastname: Yang
authors.forename: Yijun
authors.initials: Y
authors.lastname: Shu
authors.forename: Runfa
authors.initials: R
authors.lastname: Bao
authors.forename: Qichen
authors.initials: Q
authors.lastname: Ding
authors.forename: Wenguang
authors.initials: W
authors.lastname: Wu
authors.forename: Yang
authors.initials: Y
authors.lastname: Cao
authors.forename: Yingbin
authors.initials: Y
authors.lastname: Liu
mesh_terms: D048708:Cell Growth Processes / Q000502:physiology; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000737:chemistry / Q000378:metabolism; D016009:Chi-Square Distribution; D015536:Down-Regulation; D005260:Female; D005706:Gallbladder Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D036341:Intercellular Signaling Peptides and Proteins / Q000096:biosynthesis* / Q000235:genetics; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009361:Neoplasm Invasiveness; D034622:RNA Interference; D016896:Treatment Outcome
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; C051610:hepatoma-derived growth factor