title: Spatiotemporal patterns of Gem expression after rat spinal cord injury.
abstract: Gem is an atypical protein of the Ras superfamily that plays a role in regulating voltage-gated Ca(2+) channels and cytoskeletal reorganization. To elucidate the certain expression and biological function in central nervous system (CNS), we performed an acute spinal cord contusion injury model in adult rats. Western blot analysis showed a marked up-regulation of Gem after spinal cord injury (SCI). Immunohistochemistry revealed wide distribution of Gem in spinal cord, including neurons and glial cells. Double immunofluorescent staining for proliferating cell nuclear antigen (PCNA) and phenotype-specific markers indicated increases of Gem expression in proliferating microglia and astrocytes. Our data suggest that Gem may be implicated in the proliferation of microglia and astrocytes after SCI.
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, People's Republic of China.
authors.forename: Hai
authors.initials: H
authors.lastname: Wen
authors.forename: Jianhua
authors.initials: J
authors.lastname: Cao
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Yu
authors.forename: Binbin
authors.initials: B
authors.lastname: Sun
authors.forename: Tao
authors.initials: T
authors.lastname: Ding
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Debao
authors.initials: D
authors.lastname: Li
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.forename: Long
authors.initials: L
authors.lastname: Long
authors.forename: Guangfei
authors.initials: G
authors.lastname: Xu
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
mesh_terms: D000818:Animals; D039481:CD11b Antigen / Q000378:metabolism; D002452:Cell Count; D004195:Disease Models, Animal; D005786:Gene Expression Regulation / Q000502:physiology*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D008297:Male; D020559:Monomeric GTP-Binding Proteins / Q000378:metabolism*; D009457:Neuroglia / Q000378:metabolism / Q000473:pathology; D009474:Neurons / Q000378:metabolism / Q000473:pathology; D010751:Phosphopyruvate Hydratase / Q000235:genetics; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000378:metabolism* / Q000473:pathology; D013119:Spinal Cord Injuries / Q000473:pathology / Q000503:physiopathology*; D013997:Time Factors
chemical_list: D039481:CD11b Antigen; D005904:Glial Fibrillary Acidic Protein; D018809:Proliferating Cell Nuclear Antigen; C581695:Gem protein, rat; D020559:Monomeric GTP-Binding Proteins; D010751:Phosphopyruvate Hydratase