title: Conjugated polyelectrolyte materials for promoting progenitor cell growth without serum.
abstract: The discovery of new active biomaterials for promoting progenitor cell growth and differentiation in serum-free medium is still proving more challenging for the clinical treatments of degenerative diseases. In this work, a conjugated polyelectrolyte, polythiophene derivative (PMNT), was discovered to significantly drive the cell cycle progression from G₁ to S and G₂ phases and thus efficiently promote the cell growth without the need of serum. Furthermore, the fluorescent characteristic of PMNT makes it simultaneously able to trace its cellular uptake and localization by cell imaging. cDNA microarray study shows that PMNT can greatly regulate genes related to cell growth or differentiation. To the best of our knowledge, this is the first example of cell growth or differentiation promotion by polyelectrolyte material without the need of serum, thereby providing an important demonstration of degenerative biomaterial discovery through polymer design.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China.
authors.forename: Gaomai
authors.initials: G
authors.lastname: Yang
authors.forename: Libing
authors.initials: L
authors.lastname: Liu
authors.forename: Fengting
authors.initials: F
authors.lastname: Lv
authors.forename: Shu
authors.initials: S
authors.lastname: Wang
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D016895:Culture Media, Serum-Free; D005456:Fluorescent Dyes / Q000494:pharmacology; D051379:Mice; D011108:Polymers / Q000494:pharmacology*; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects*; D013876:Thiophenes / Q000494:pharmacology*
chemical_list: D001672:Biocompatible Materials; D016895:Culture Media, Serum-Free; D005456:Fluorescent Dyes; D011108:Polymers; D013876:Thiophenes; C066730:polythiophene