title: [Effect on different concentrations of exogenous hormones on baicalin in Scutellaria baicalensis callus].
abstract: OBJECTIVE
  To determine the content of baicalin in Scutellaria baicalensis callus induced by different doncentrations of exogenous hormones.
  
  
  METHODS
  HPLC system was adopted to determine baicalin in S. baicalensis callus. Chromatographic conditions: ODS column was adopted, with methanol-water-phosphate (47: 53: 0.2) as the mobile phase. The flow velocity was 1 mL x min(-1), the detective wavelength was 280 nm, and the temperature of column was room temperature.
  
  
  RESULTS
  S. baicalensis callus induced by 6-BA 1.0 mg x L(-1) + NAA 0.5 mg x L(-1) showed the highest baicalin content, up to 49.78 mg x g(-1).
  
  
  CONCLUSIONS
  The experiment is such a simple, rapid and stable method for determining the baicalin content that it can be used for determining the baicalin content in S. baicalensis callus.
authors.affiliation: Tianjin University of Traditional Chiness Medicine, Tianjin 300193, China. wgx0502@126.com
authors.forename: Gui-Xiang
authors.initials: GX
authors.lastname: Wan
authors.forename: Lin
authors.initials: L
authors.lastname: Ma
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
mesh_terms: D015084:2,4-Dichlorophenoxyacetic Acid / Q000494:pharmacology; D001593:Benzyl Compounds; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000378:metabolism*; D007701:Kinetin / Q000494:pharmacology; D009280:Naphthaleneacetic Acids / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000494:pharmacology*; D011687:Purines; D031364:Scutellaria baicalensis / Q000187:drug effects* / Q000378:metabolism; D046509:Tissue Culture Techniques / Q000379:methods*
chemical_list: D001593:Benzyl Compounds; D005419:Flavonoids; D009280:Naphthaleneacetic Acids; D010937:Plant Growth Regulators; D011687:Purines; D015084:2,4-Dichlorophenoxyacetic Acid; C038044:baicalin; C480551:benzylaminopurine; D007701:Kinetin