title: NOX, the main regulator in oxidative stress in experimental models of phenylketonuria?
abstract: OBJECTIVE
  To reveal the role of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) in the integration of the redox signal in the oxidative molecular regulation mechanism in phenylketonuria (PKU).
  
  
  METHODS
  The blood samples were obtained from Pahenu2-BTBR PKU and wild-type mice, respectively. Phe concentration, total antioxidant capacity (T-AOC), glutathione (GSH) and maleic dialdehyde (MDA) were analyzed. After collection of the mononuclear cells, reverse transcription polymerase chain reaction (RT-PCR) for NOX was performed. In addition, NOX activity and superoxide in mononuclear cells were determined.
  
  
  RESULTS
  Compared to the control group, Phe concentration, T-AOC and MDA were markedly increased in PKU mice (p<0.01, p<0.05, p<0.01, respectively). However, the GSH level in PKU mice was less than that in control group (p<0.05). The mRNA level of subunits of NOX included p47phox and p67phox, were increased in PKU mice (p<0.05), however, the gp91phox had no obvious change in the two groups (p>0.05). NOX activity and superoxide were also remarkably elevated in PKU mice (p<0.05).
  
  
  CONCLUSIONS
  NOX may play an important role in the integration of the redox signal in the oxidative molecular regulation mechanism in PKU.
authors.forename: Yingzhong
authors.initials: Y
authors.lastname: He
authors.forename: Xuefan
authors.initials: X
authors.lastname: Gu
authors.forename: Lihua
authors.initials: L
authors.lastname: Lu
authors.forename: Lili
authors.initials: L
authors.lastname: Liang
authors.forename: Jialin
authors.initials: J
authors.lastname: Gao
authors.forename: Xinshun
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005978:Glutathione / Q000097:blood; D051379:Mice; D019255:NADPH Oxidases / Q000502:physiology*; D018384:Oxidative Stress; D010649:Phenylalanine / Q000097:blood; D010661:Phenylketonurias / Q000378:metabolism*; D013481:Superoxides / Q000097:blood
chemical_list: D013481:Superoxides; D010649:Phenylalanine; D019255:NADPH Oxidases; D005978:Glutathione