title: Separation and characterization of ciprofloxacin, difloxacin, lomefloxacin, norfloxacin, and ofloxacin oxidation products under potassium permanganate treatment in acidic medium by UPLC-MS/MS.
abstract: A simple, sensitive and reproducible ultra-performance liquid chromatography method for determination of ciprofloxacin, difloxacin, lomefloxacin, norfloxacin and ofloxacin oxidation stability under permanganate treatment in acidic conditions at pH from 3.0 to 6.0, was developed. Chromatographic separations were carried out using the Acquity UPLC BEH C18 column; (2.1×100 mm, 1.7 μm particle size). The column was maintained at 40°C, and eluted under isocratic conditions using 83% of eluent A and 17% of eluent B over 6.5 min, at a flow rate of 0.3 mL min(-1). Eluent A: water/formic acid (0.1 v/v%); eluent B: acetonitrile/formic acid (0.1 v/v%). An oxidation process followed kinetic of the second order reaction and depended upon solution acidity. Oxidation of fluoroquinolones proceeded at piperazine moiety yielding respective hydroxy and oxo analogs, and remaining the quinolone fragment intact. Structures of products formed were assigned on a basis of UPLC/MS/MS fragmentation pathways.
authors.affiliation: Department of Inorganic and Analytical Chemistry, Jagiellonian University Medical College, Faculty of Pharmacy, Kraków, Poland.
authors.forename: Urszula
authors.initials: U
authors.lastname: Hubicka
authors.forename: Paweł
authors.initials: P
authors.lastname: Zmudzki
authors.forename: Barbara
authors.initials: B
authors.lastname: Zuromska-Witek
authors.forename: Paweł
authors.initials: P
authors.lastname: Zajdel
authors.forename: Maciej
authors.initials: M
authors.lastname: Pawłowski
authors.forename: Jan
authors.initials: J
authors.lastname: Krzek
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D004355:Drug Stability; D024841:Fluoroquinolones / Q000737:chemistry / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D015394:Molecular Structure; D010084:Oxidation-Reduction; D011196:Potassium Permanganate / Q000737:chemistry*; D015203:Reproducibility of Results; D012996:Solutions; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D012996:Solutions; D011196:Potassium Permanganate