title: A general synthetic method toward uridylylated picornavirus VPg proteins.
abstract: Small proteins called viral protein genome-linked (VPg), attached to the 5'-end of the viral RNA genome are found as common structure in the large family of picornaviruses. The replication of these viruses is primed by this VPg protein linked to a single uridylyl residue. We report a general procedure to obtain such nucleoproteins employing a pre-uridylylated tyrosine building block in an on-line solid phase-based approach.
authors.affiliation: Leiden Institute of Chemistry, Leiden University, P. O. Box 9502, RA, 2300, Leiden, The Netherlands.
authors.forename: Gerbrand J
authors.initials: GJ
authors.lastname: van der Heden van Noort
authors.forename: Catherine H
authors.initials: CH
authors.lastname: Schein
authors.forename: Herman S
authors.initials: HS
authors.lastname: Overkleeft
authors.forename: Gijsbert A
authors.initials: GA
authors.lastname: van der Marel
authors.forename: Dmitri V
authors.initials: DV
authors.lastname: Filippov
mesh_terms: D015394:Molecular Structure; D010849:Picornaviridae / Q000737:chemistry*; D014443:Tyrosine / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D014529:Uridine / Q000737:chemistry* / Q000378:metabolism; D014764:Viral Proteins / Q000737:chemistry* / Q000378:metabolism*
chemical_list: D014764:Viral Proteins; D014443:Tyrosine; D014529:Uridine