title: [Extraction technology and antioxidant activity of total flavonoids and total triterpenoids from Tetrastigma planicaule].
abstract: OBJECTIVE
  To study the optimum extraction technology of total flavonoids and total triterpenoids from Tetrastigma planicaule and their antioxidant activity.
  
  
  METHODS
  Optimized the extraction of total flavonoids and total triterpenoids using an L9 (3(4)) orthogonal array design, and the antioxidant activity was extimated by FRAP assay, salicylicl acid assay and ABTS assay.
  
  
  RESULTS
  The best extraction conditions for total flavonoids from Tetrastigma planicaule were as follows: 70 degrees C of 70% ethanol ultrasound-assisted extracting for 1 h and extracting three times, and total triterpenoids was:70 degrees C of 60% ethanol microwave extracting for 5 min and extracting two times. Compared with the positive control samples VC and tea polyphenol, the activity of reducing Fe3+ antioxidant and scavenging ABTS + * of flavonoids from Tetrastigma planicaule was higher than that of tea polyphenol, but scavenging OH * was lower than theirs;
  
  
  CONCLUSIONS
  The method of using ultrasound-assisted extraction to extract total flavonoids and microwave extraction to extract total triterpenoids is the best, the extracts of Tetrastigma planicaule also shows certain antioxidative activity.
authors.affiliation: Youjiang Medical University for Nationalities, Baise 533000, China. panqiao112@163.com
authors.forename: Qiao-Dan
authors.initials: QD
authors.lastname: Pan
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Xiong
authors.forename: Wen-Dong
authors.initials: WD
authors.lastname: Chen
authors.forename: Qing-Hua
authors.initials: QH
authors.lastname: Du
authors.forename: Jin-Jue
authors.initials: JJ
authors.lastname: Li
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D000431:Ethanol / Q000737:chemistry; D005419:Flavonoids / Q000302:isolation & purification* / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology; D008872:Microwaves; D010084:Oxidation-Reduction / Q000187:drug effects; D018547:Plant Stems / Q000737:chemistry; D012997:Solvents; D013678:Technology, Pharmaceutical / Q000379:methods*; D014315:Triterpenes / Q000302:isolation & purification* / Q000494:pharmacology; D014465:Ultrasonics; D027844:Vitaceae / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D016166:Free Radical Scavengers; D012997:Solvents; D014315:Triterpenes; D000431:Ethanol