title: Dietary organic selenium improves growth, survival and resistance to Vibrio mimicus in cultured marron, Cherax cainii (Austin, 2002).
abstract: To determine the effects of dietary organic selenium (OS) supplementation on the growth performance and immune competence of marron, Cherax cainii (Austin, 2002), a group of marron were fed 0.2 g kg(-1) of Sel-Plex(®) supplemented basal diet and then compared with another group (control) of marron fed basal diet without any supplementation. After 90 days of feeding, final weight, average weekly gains (AWG), relative gain rate (RGR), specific growth rate (SGR), survival, total and differential haemocyte counts (THC and DHC), were compared between the two groups. Surviving marron from each group were then divided into three sub-groups (three tanks per sub-group with seven marron per tank); (1) first sub-group was injected with 20 μL of 3.24 × 10(6) cfu Vibrio mimicus; (2) the second sub-group was injected with 20 μL normal saline and (3) the third sub-group was not subjected to injection and became the control group. THC, DHC, neutral red retention time (NRRT) and Vibrio ranks of post-injected marron were evaluated for 96 h, at every 24-h interval. The results showed that after 90 days of feeding, final weight, AWG, RGR, SGR, survival, THC, proportion of hyaline cells of OS-fed marron were significantly higher (P < 0.05) than the control group, whereas proportion of granular and semigranular cells were not affected by dietary OS. After challenging with V. mimicus, survival rate of marron without dietary OS significantly decreased (P < 0.05) as compared to the control group of marron. THC of marron in all sub-groups were significantly reduced (P < 0.05) after the challenge. However, THC and granular cells of sub-groups fed OS were higher than other sub-groups. Vibrio ranks and NRRT of marron fed OS were significantly lower and slower, respectively, than marron fed without OS. These findings demonstrated the benefits of OS inclusion in the marron diet in terms of growth, health and disease resistance.
authors.affiliation: Sustainable Aquatic Resources and Biotechnology, Department of Environment and Agriculture, School of Science, Curtin University, Perth, Western Australia 6845, Australia. rudysatriana@yahoo.com
authors.forename: Rudy Agung
authors.initials: RA
authors.lastname: Nugroho
authors.forename: Ravi
authors.initials: R
authors.lastname: Fotedar
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D017756:Aquaculture; D003400:Astacoidea / Q000187:drug effects* / Q000254:growth & development / Q000276:immunology / Q000502:physiology; D019587:Dietary Supplements / Q000032:analysis; D007113:Immunity, Innate; D016566:Organoselenium Compounds / Q000008:administration & dosage*; D044164:Vibrio mimicus / Q000502:physiology
chemical_list: D016566:Organoselenium Compounds