title: Rnh1 promotes differentiation and myelination via RhoA in oligodendrocytes.
abstract: Increases in Rattus norvegicus ribonuclease/angiogenin inhibitor 1 (Rnh1) are observed in rat primary neuron injury and/or the regeneration process and in differentiated oligodendrocytes. However, the roles of Rnh1 in the central nervous system are still largely unexplored. RhoA is an important signaling protein that has been implicated in oligodendrocyte differentiation and myelination. We demonstrate enhanced differentiation and myelination of oligodendrocytes mediated by Rnh1 in vitro. We further show that Rnh1 is expressed in oligodendrocyte precursors and oligodendrocytes. Importantly, Rnh1 strongly affects oligodendrocyte differentiation through RhoA-ROCK signaling. Moreover, changes in Rnh1 expression in oligodendrocytes regulates the expression and phosphorylation of Fyn, a regulator of RhoA activity. Finally, Rnh1 promotes myelination in vitro. These results show that Rnh1-mediated RhoA inactivation enhances the differentiation and myelination in oligodendrocytes. Overall, Rnh1 might contribute to oligodendrocyte differentiation and myelination processes in vitro.
authors.affiliation: State Key Laboratory of Proteomics and Department of Neurobiology, Institute of Basic Medical Sciences, The Academy of Military Medical Sciences, Beijing, 100850, People's Republic of China.
authors.forename: C F
authors.initials: CF
authors.lastname: Zhao
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: H P
authors.initials: HP
authors.lastname: Que
authors.forename: S G
authors.initials: SG
authors.lastname: Yang
authors.forename: T
authors.initials: T
authors.lastname: Liu
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Liu
authors.forename: H D
authors.initials: HD
authors.lastname: Hui
authors.forename: Shaojun
authors.initials: S
authors.lastname: Liu
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000502:physiology; D009186:Myelin Sheath / Q000235:genetics / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009836:Oligodendroglia / Q000166:cytology / Q000378:metabolism*; D010766:Phosphorylation / Q000502:physiology; D051076:Proto-Oncogene Proteins c-fyn / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015398:Signal Transduction / Q000502:physiology*; D054460:rho-Associated Kinases / Q000235:genetics / Q000378:metabolism; D020742:rhoA GTP-Binding Protein / Q000235:genetics / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D009419:Nerve Tissue Proteins; C028666:Rnh1 protein, rat; C494961:Fyn protein, rat; D051076:Proto-Oncogene Proteins c-fyn; D054460:rho-Associated Kinases; D020742:rhoA GTP-Binding Protein