title: Analysis of genomic mutation and immunohistochemistry of platelet-derived growth factor receptors in canine vascular tumours.
abstract: We examined whether mutation of the platelet-derived growth factor receptor protein tyrosine kinase (PDGFR)-α and PDGFR-β genes contributes to their overexpression in canine vascular tumours. Genomic sequences of trans- or juxtamembrane regions of PDGFR-α and PDGFR-β were analysed with immunohistochemical staining and polymerase chain reaction-direct sequencing using DNA from paraffin-embedded neoplastic tissues of 27 hemangiosarcomas (HSAs) and 20 hemangiomas (HAs). Immunohistochemically, 75% of the HA cases were positive for PDGFR-α and almost most of the HA cases were negative for PDGFR-β. Of the HSA cases, 55.6% were negative for PDGFR-α and 63% were strongly positive for PDGFR-β. Among the HA cases, 1 missense mutation was detected in PDGFR-α exon 18 and 1 in PDGFR-β exon 17. Two HSA cases had missense mutations in exon 14 and 1 in exon 17 of PDGFR-β. Thus, genomic mutation of trans- or juxtamembrane regions of PDGFRs was not the main mechanism driving the activation of receptors in HSA and HA.
authors.affiliation: Laboratory of Veterinary Pathology, Department of Veterinary Medicine, Gifu University, Gifu, Japan.|Department of Veterinary Pathology, Faculty of Veterinary Medicine, Kafr El-Sheikh, Egypt.
authors.forename: S
authors.initials: S
authors.lastname: Abou Asa
authors.affiliation: Laboratory of Clinical Oncology, Department of Veterinary Medicine, Gifu University, Gifu, Japan.|Comparative Cancer Center, Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Mori
authors.affiliation: Laboratory of Clinical Oncology, Department of Veterinary Medicine, Gifu University, Gifu, Japan.|Comparative Cancer Center, Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Maruo
authors.affiliation: Department of Veterinary Pathology, Faculty of Veterinary Medicine, Kafr El-Sheikh, Egypt.
authors.forename: A
authors.initials: A
authors.lastname: Khater
authors.affiliation: Laboratory of Veterinary Pathology, Department of Veterinary Medicine, Gifu University, Gifu, Japan.
authors.forename: A
authors.initials: A
authors.lastname: El-Sawak
authors.affiliation: Department of Veterinary Pathology, Faculty of Veterinary Medicine, Kafr El-Sheikh, Egypt.
authors.forename: E
authors.initials: E
authors.lastname: Abd el-Aziz
authors.affiliation: Laboratory of Veterinary Pathology, Department of Veterinary Medicine, Gifu University, Gifu, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yanai
authors.affiliation: Laboratory of Veterinary Pathology, Department of Veterinary Medicine, Gifu University, Gifu, Japan.|Comparative Cancer Center, Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Sakai
mesh_terms: D000818:Animals; D017931:DNA Primers; D004283:Dog Diseases / Q000235:genetics*; D004285:Dogs; D005260:Female; D006391:Hemangioma / Q000235:genetics / Q000662:veterinary*; D006394:Hemangiosarcoma / Q000235:genetics / Q000662:veterinary*; D007150:Immunohistochemistry / Q000662:veterinary; D007564:Japan; D008297:Male; D009154:Mutation; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011505:Protein-Tyrosine Kinases / Q000235:genetics; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000032:analysis / Q000235:genetics*; D020797:Receptor, Platelet-Derived Growth Factor beta / Q000032:analysis / Q000235:genetics*; D019043:Vascular Neoplasms / Q000235:genetics / Q000662:veterinary*
chemical_list: D017931:DNA Primers; D011505:Protein-Tyrosine Kinases; D020796:Receptor, Platelet-Derived Growth Factor alpha; D020797:Receptor, Platelet-Derived Growth Factor beta
keywords: PDGFR; dog; hemangioma; hemangiosarcoma; immunohistochemistry; mutation