title: Intra-axonal synthesis of eukaryotic translation initiation factors regulates local protein synthesis and axon growth in rat sympathetic neurons.
abstract: Axonal protein synthesis is a complex process involving selective mRNA localization and translational regulation. In this study, using in situ hybridization and metabolic labeling, we show that the mRNAs encoding eukaryotic translation initiation factors eIF2B2 and eIF4G2 are present in the axons of rat sympathetic neurons and are locally translated. We also report that a noncoding microRNA, miR16, modulates the axonal expression of eIF2B2 and eIF4G2. Transfection of axons with precursor miR16 and anti-miR16 showed that local miR16 levels modulated axonal eIF2B2 and eIF4G2 mRNA and protein levels, as well as axon outgrowth. siRNA-mediated knock-down of axonal eIF2B2 and eIF4G2 mRNA also resulted in a significant decrease in axonal eIF2B2 and eIF4G2 protein. Moreover, results of metabolic labeling studies showed that downregulation of axonal eIF2B2 and eIF4G2 expression also inhibited local protein synthesis and axon growth. Together, these data provide evidence that miR16 mediates axonal growth, at least in part, by regulating the local protein synthesis of eukaryotic translation initiation factors eIF2B2 and eIF4G2 in the axon.
authors.affiliation: Laboratory of Molecular Biology, National Institute of Mental Health, National Institutes of Health, Bethesda, Maryland 20892-1381, USA.
authors.forename: Amar N
authors.initials: AN
authors.lastname: Kar
authors.forename: Margaret A
authors.initials: MA
authors.lastname: MacGibeny
authors.forename: Noreen M
authors.initials: NM
authors.lastname: Gervasi
authors.forename: Anthony E
authors.initials: AE
authors.lastname: Gioio
authors.forename: Barry B
authors.initials: BB
authors.lastname: Kaplan
mesh_terms: D000320:Adrenergic Fibers / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D001369:Axons / Q000378:metabolism* / Q000502:physiology; D002478:Cells, Cultured; D015536:Down-Regulation / Q000502:physiology; D020717:Eukaryotic Initiation Factor-2B / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000502:physiology; D039603:Eukaryotic Initiation Factor-4G / Q000037:antagonists & inhibitors / Q000096:biosynthesis* / Q000502:physiology; D005260:Female; D008297:Male; D014176:Protein Biosynthesis / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017783:Superior Cervical Ganglion / Q000378:metabolism / Q000502:physiology
chemical_list: C491300:EIF4G2 protein, human; D020717:Eukaryotic Initiation Factor-2B; D039603:Eukaryotic Initiation Factor-4G