title: Use of a commercial enzyme-linked immunosorbent assay for rapid detection of Giardia duodenalis in dog stools in the environment: a Bayesian evaluation.
abstract: Giardia duodenalis is considered a potentially zoonotic protozoan. Some animal species, including infected dogs, may play an important role in the spread of Giardia cysts through environmental contamination with their feces. In the present study, a commercial enzyme-linked immunosorbent assay (ELISA) was used to examine 143 samples of dog feces collected in urban areas as an indicator of the risk of field contamination. Using a Bayesian statistical approach, the ELISA showed a sensitivity of 88.9% and a specificity of 95.8% with positive and negative predictive values of 89.6% and 95.4%, respectively. The test affords the advantage of rapid processing of fecal samples without a complex technical structure and extensive costly labor. Moreover, the present results show that the assay provides public health veterinarians with a practical tool that can be used in screening programs, as a valid alternative or as an adjunct to other tests, in order to assess the biological risk of exposure to G. duodenalis cysts from dogs in human settlements. However, the test may not be completely accurate for human health risk evaluation, as it does not discriminate between zoonotic and non-zoonotic isolates.
authors.affiliation: Department of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, Italy. rpapini@vet.unipi.it
authors.forename: Roberto
authors.initials: R
authors.lastname: Papini
authors.forename: Giulia
authors.initials: G
authors.lastname: Carreras
authors.forename: Marianna
authors.initials: M
authors.lastname: Marangi
authors.forename: Francesca
authors.initials: F
authors.lastname: Mancianti
authors.forename: Annunziata
authors.initials: A
authors.lastname: Giangaspero
mesh_terms: D000818:Animals; D001499:Bayes Theorem; D004283:Dog Diseases / Q000469:parasitology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005243:Feces / Q000469:parasitology*; D016829:Giardia lamblia / Q000302:isolation & purification*; D005873:Giardiasis / Q000175:diagnosis / Q000662:veterinary*; D006801:Humans; D012680:Sensitivity and Specificity; D015047:Zoonoses
keywords: Bayesian analysis; Giardia duodenalis; dog stools; environment; enzyme-linked immunosorbent assay