title: Expression of NF-κB and osteopontin of synovial fluid of patients with knee osteoarthritis.
abstract: OBJECTIVE
  To explore the significance of osteopontin and nuclear factor κB (NF-κB) expression in patients with knee osteoarthritis.
  
  
  METHODS
  RT-PCR and enzyme-linked immunosorbent assay were used to measure the Osteopontin (OPN) and NF-κB concentration of knee joint synovial fluid of patients with knee osteoarthritis and trauma fractures, and analyze the relationship between the expressiones of them.
  
  
  RESULTS
  OPN and NF-κB expression at the mRNA and protein levels of patients with knee osteoarthritis were significantly higher than the control group. the result showed statistical significance (P<0.05). There was a positive correlation between the OPN levels in synovial fluid of patients with knee osteoarthritis and NF-κB expression levels (P<0.05).
  
  
  CONCLUSIONS
  The high expression of OPN and NF-κB are closely related to occurrence and development of knee osteoarthritis.
authors.affiliation: Department of Orthopedics, Second Xiang-Ya Hospital, Central South University, Changsha, Hunan, China 410010.
authors.forename: Li-Feng
authors.initials: LF
authors.lastname: Qin
authors.forename: Wan-Chun
authors.initials: WC
authors.lastname: Wang
authors.forename: Han
authors.initials: H
authors.lastname: Fang
authors.forename: Xin-Zhan
authors.initials: XZ
authors.lastname: Mao
authors.forename: Guo-Liang
authors.initials: GL
authors.lastname: Huang
authors.forename: You
authors.initials: Y
authors.lastname: Chen
authors.forename: Hou-De
authors.initials: HD
authors.lastname: Zhou
authors.forename: Yi
authors.initials: Y
authors.lastname: Shen
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Qin
authors.forename: Dan
authors.initials: D
authors.lastname: Peng
mesh_terms: D000328:Adult; D000368:Aged; D015153:Blotting, Western; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D020370:Osteoarthritis, Knee / Q000378:metabolism*; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018709:Statistics, Nonparametric; D013582:Synovial Fluid / Q000378:metabolism*
chemical_list: D016328:NF-kappa B; D012333:RNA, Messenger; D053495:Osteopontin