title: Graphene oxide as nanocarrier for sensitive electrochemical immunoassay of clenbuterol based on labeling amplification strategy.
abstract: A novel electrochemical immunosensor for sensitive detection of clenbuterol (CLB) is fabricated using glucose oxidase (GOD)-functionalized grahene oxide (GO) nanocomposites to label CLB. The immunosensor was constructed by layer-by-layer assembly colloidal prussian blue (PB), multiwalled carbon nanotubes (MWCNTs) and CLB antibodies (Abs) on a glassy carbon electrode (GCE). In this competitive immunoassay system, PB acts as the redox mediator to reduce H2O2 originated from the catalyst cycle of GOD. The high ratio of GOD to GO effectively amplified the signal for this competitive-type immunoassay. Under optimized conditions, the immunosensor shows a wide linear range from 0.5 to 1,000 ng/mL with a low detection limit of 0.25 ng/mL. The dual signal amplification of GOD-functionalized GO nanocomposites as a label is promising to be applied to design other sensitive immunosenseors.
authors.affiliation: Department of Chemistry, East China Normal University, Shanghai 200062, PR China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Lai
authors.forename: Jing
authors.initials: J
authors.lastname: Bai
authors.forename: Xinhao
authors.initials: X
authors.lastname: Shi
authors.forename: Yanbo
authors.initials: Y
authors.lastname: Zeng
authors.forename: Yuezhong
authors.initials: Y
authors.lastname: Xian
authors.forename: Jie
authors.initials: J
authors.lastname: Hou
authors.forename: Litong
authors.initials: L
authors.lastname: Jin
mesh_terms: D000318:Adrenergic beta-Agonists / Q000032:analysis*; D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D001234:Aspergillus niger / Q000201:enzymology; D002976:Clenbuterol / Q000032:analysis*; D055664:Electrochemical Techniques / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry; D010087:Oxides / Q000737:chemistry*; D013552:Swine
chemical_list: D000318:Adrenergic beta-Agonists; D004800:Enzymes, Immobilized; D010087:Oxides; D006046:Gold; D006108:Graphite; D005949:Glucose Oxidase; D002976:Clenbuterol