title: Dynamics of PEGylated-dextran-spermine nanoparticles for gene delivery to leukemic cells.
abstract: Leukemic cells are hard-to-transfect cell lines. Many transfection reagents which can provide high gene transfer efficiency in common adherent cell lines are not effective to transfect established blood cell lines or primary leukemic cells. This study aims to examine a new class of cationic polymer non-viral vector, PEGylated-dextran-spermine (PEG-D-SPM), to determine its ability to transfect the leukemic cells. Here, the optimal conditions of the complex preparation (PEG-D-SPM/plasmid DNA (pDNA)) were examined. Different weight-mixing (w/w) ratios of PEG-D-SPM/pDNA complex were prepared to obtain an ideal mixing ratio to protect encapsulated pDNA from DNase degradation and to determine the optimal transfection efficiency of the complex. Strong complexation between polymer and pDNA in agarose gel electrophoresis and protection of pDNA from DNase were detected at ratios from 25 to 15. Highest gene expression was detected at w/w ratio of 18 in HL60 and K562 cells. However, gene expression from both leukemic cell lines was lower than the control MCF-7 cells. The cytotoxicity of PEG-D-SPM/pDNA complex at the most optimal mixing ratios was tested in HL60 and K562 cells using MTS assay and the results showed that the PEG-D-SPM/pDNA complex had no cytotoxic effect on these cell lines. Spherical shape and nano-nature of PEG-D-SPM/pDNA complex at ratio 18 was observed using transmission electron microscopy. As PEG-D-SPM showed modest transfection efficiency in the leukemic cell lines, we conclude that further work is needed to improve the delivery efficiency of the PEG-D-SPM.
authors.affiliation: Medical Genetics Laboratory, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
authors.forename: R
authors.initials: R
authors.lastname: Amini
authors.forename: F Azizi
authors.initials: FA
authors.lastname: Jalilian
authors.forename: S
authors.initials: S
authors.lastname: Abdullah
authors.forename: A
authors.initials: A
authors.lastname: Veerakumarasivam
authors.forename: H
authors.initials: H
authors.lastname: Hosseinkhani
authors.forename: A S
authors.initials: AS
authors.lastname: Abdulamir
authors.forename: A J
authors.initials: AJ
authors.lastname: Domb
authors.forename: D
authors.initials: D
authors.lastname: Ickowicz
authors.forename: R
authors.initials: R
authors.lastname: Rosli
mesh_terms: D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D015973:Gene Expression Regulation, Leukemic; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D018922:HL-60 Cells; D006801:Humans; D020014:K562 Cells; D061986:MCF-7 Cells; D046911:Macromolecular Substances / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D010957:Plasmids / Q000737:chemistry / Q000235:genetics; D011092:Polyethylene Glycols / Q000737:chemistry / Q000494:pharmacology*; D013096:Spermine / Q000737:chemistry / Q000494:pharmacology*; D014162:Transfection
chemical_list: D046911:Macromolecular Substances; D013096:Spermine; D011092:Polyethylene Glycols