title: Fluorescent properties of oligonucleotides doubly modified with an indole-fused cytosine analog and 2-aminopurine.
abstract: Single- and double-stranded oligodeoxynucleotides (ODNs) incorporating both 2-aminopurine (2AP) and an indole-fused cytosine analog (PPI) were prepared and studied for their fluorescence properties. PPI and 2AP can be excited simultaneously by irradiation at 300 nm, with emission observed at 500 nm for PPI and 370 nm for 2AP. We demonstrated the utility of these properties in the dual fluorescence labeling of ODNs giving well-separated emission peaks. In addition, both of the fluorescence signals of a doubly modified ODN changed independently, reflecting the local duplex formation at the regions containing 2AP or PPI. Potential applications of this strategy for the dual fluorescence labeling of oligonucleotides with 2AP and PPI include monitoring local structure alterations of functional nucleic acids and the multiplex detection of biologically important nucleic acids.
authors.affiliation: Department of Life Science, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8501, Japan. kseio@bio.titech.ac.jp
authors.forename: Kohji
authors.initials: K
authors.lastname: Seio
authors.forename: Takashi
authors.initials: T
authors.lastname: Kanamori
authors.forename: Munefumi
authors.initials: M
authors.lastname: Tokugawa
authors.forename: Hiroki
authors.initials: H
authors.lastname: Ohzeki
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Masaki
authors.forename: Hirosuke
authors.initials: H
authors.lastname: Tsunoda
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ohkubo
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Sekine
mesh_terms: D015075:2-Aminopurine / Q000031:analogs & derivatives* / Q000737:chemistry*; D001483:Base Sequence; D003596:Cytosine / Q000031:analogs & derivatives* / Q000737:chemistry*; D005456:Fluorescent Dyes; D007211:Indoles / Q000737:chemistry*; D057054:Molecular Imaging; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D007211:Indoles; D009841:Oligonucleotides; D015075:2-Aminopurine; D003596:Cytosine