title: The effects of glutathione on malondialdehyde expression and seminiferous tubule damage in experimental testicular torsion-detorsion in Wistar rats.
abstract: BACKGROUND
  Testicular damage caused by ischemia reperfusion injury can be determined by malondialdehyde (MDA) expression and grading of the histopathological damage to seminiferous tubules. The aim of this study was to investigate the effect of Tationil Glutathione administration on testicular damage following experimental torsion and detorsion.
  
  
  METHODS
  Eighteen Wistar albino rats, 5.5-6 months old and weighing 250-300 g, were divided into three equal groups. In the first group (T), torsion was created by rotating the left testis 720° in a clockwise direction and maintained for 4 h. In the T/D group, after 4 h of torsion, detorsion was performed and maintained for 3 h. In the T/D-GLUT group, we injected 25 mg iv gluthatione before performing detorsion (onset time of agent is ±5 min).
  
  
  RESULTS
  The lowest malondialdehyde (MDA) expression was observed in the T/D-GLUT rats (P < 0.05). Grading of the histopathological damage to seminiferous tubules showed the damage to be worst in T/D and least in T/D-GLUT rats (P < 0.05).
  
  
  CONCLUSIONS
  Tationil Glutathione inhibits formation of reactive oxygen species in testicular tissue during ischemia and reperfusion injury caused by experimental torsion and detorsion in Wistar rats.
authors.affiliation: Division of Pediatric Surgery, Faculty of Medicine, Padjadjaran University, Hasan Sadikin General Hospital, Bandung, Indonesia. Electronic address: runbilommi@live.com.
authors.forename: Ruankha
authors.initials: R
authors.lastname: Bilommi
authors.forename: Bustanul A
authors.initials: BA
authors.lastname: Nawas
authors.forename: Dikki D
authors.initials: DD
authors.lastname: Kusmayadi
authors.forename: Rizki
authors.initials: R
authors.lastname: Diposarosa
authors.forename: Arhans
authors.initials: A
authors.lastname: Chairul
authors.forename: Bethy S
authors.initials: BS
authors.lastname: Hernowo
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005978:Glutathione / Q000494:pharmacology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D012671:Seminiferous Tubules / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013086:Spermatic Cord Torsion / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D013737:Testis / Q000187:drug effects / Q000378:metabolism / Q000473:pathology
chemical_list: D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D005978:Glutathione
keywords: Experimental testicular torsion; Ischemia reperfusion injury