title: The responses of γδ T-cells against acute Pseudomonas aeruginosa pulmonary infection in mice via interleukin-17.
abstract: Interleukin (IL)-17-producing T-lymphocytes play a crucial role in inflammation, yet the potential roles of the cells in acute bacterial pulmonary infection remain unclear. Here, we investigated the role of IL-17-producing γδ T-cells in a mouse model of acute Pseudomonas aeruginosa pulmonary infection. Results showed that augmentation of IL-17, IL-22 and IL-23 was associated with the development of acute bacterial pulmonary infection. However, IL-17 was markedly reduced following the blockade of γδ T-cell activity in vivo. The levels of the chemokines, including granulocyte colony-stimulating factor (G-CSF), keratinocyte chemoattractant (KC), macrophage inflammatory protein-1α (MIP-1) and macrophage inflammatory protein (MIP-2), were also noticeably decreased in the anti-γδ T Cell Receptor(TCR) mice after 8 h infection. Following the depletion of γδ T-cells, the bacterial load was consistently increased. Anti-TCRγδ-treated mice had changes similar to those in the the anti-IL-17-treated mice. The mRNA and protein levels of IL-22 and IL-23, and the mRNA level of RORγt were all markedly decreased in the anti-TCRγδ mice. Overall, our results demonstrated that at the early stage of acute P. aeruginosa pulmonary infection, γδ T-cells are the major source of IL-17 and play a pivotal role in the host immune response and defense against bacteria.
authors.affiliation: Department of Pulmonary Medicine, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors.forename: Jialin
authors.initials: J
authors.lastname: Liu
authors.forename: Hongping
authors.initials: H
authors.lastname: Qu
authors.forename: Qingyun
authors.initials: Q
authors.lastname: Li
authors.forename: Liang
authors.initials: L
authors.lastname: Ye
authors.forename: Guanhua
authors.initials: G
authors.lastname: Ma
authors.forename: Huanying
authors.initials: H
authors.lastname: Wan
mesh_terms: D000818:Animals; D058491:Bacterial Load; D004195:Disease Models, Animal; D020381:Interleukin-17 / Q000276:immunology*; D047589:Leukocyte Reduction Procedures; D008168:Lung / Q000276:immunology / Q000382:microbiology; D051379:Mice; D008810:Mice, Inbred C57BL; D018410:Pneumonia, Bacterial / Q000276:immunology* / Q000382:microbiology; D011552:Pseudomonas Infections / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D013047:Specific Pathogen-Free Organisms; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D020381:Interleukin-17