title: EBE, an AP2/ERF transcription factor highly expressed in proliferating cells, affects shoot architecture in Arabidopsis.
abstract: We report about ERF BUD ENHANCER (EBE; At5g61890), a transcription factor that affects cell proliferation as well as axillary bud outgrowth and shoot branching in Arabidopsis (Arabidopsis thaliana). EBE encodes a member of the APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF) transcription factor superfamily; the gene is strongly expressed in proliferating cells and is rapidly and transiently up-regulated in axillary meristems upon main stem decapitation. Overexpression of EBE promotes cell proliferation in growing calli, while the opposite is observed in EBE-RNAi lines. EBE overexpression also stimulates axillary bud formation and outgrowth, while repressing it results in inhibition of bud growth. Global transcriptome analysis of estradiol-inducible EBE overexpression lines revealed 48 EBE early-responsive genes, of which 14 were up-regulated and 34 were down-regulated. EBE activates several genes involved in cell cycle regulation and dormancy breaking, including D-type cyclin CYCD3;3, transcription regulator DPa, and BRCA1-ASSOCIATED RING DOMAIN1. Among the down-regulated genes were DORMANCY-ASSOCIATED PROTEIN1 (AtDRM1), AtDRM1 homolog, MEDIATOR OF ABA-REGULATED DORMANCY1, and ZINC FINGER HOMEODOMAIN5. Our data indicate that the effect of EBE on shoot branching likely results from an activation of genes involved in cell cycle regulation and dormancy breaking.
authors.affiliation: Max-Planck Institute of Molecular Plant Physiology, Cooperative Research Group, D-14476 Golm/Potsdam, Germany.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Mehrnia
authors.forename: Salma
authors.initials: S
authors.lastname: Balazadeh
authors.forename: María-Inés
authors.initials: MI
authors.lastname: Zanor
authors.forename: Bernd
authors.initials: B
authors.lastname: Mueller-Roeber
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics; D049109:Cell Proliferation; D016213:Cyclins / Q000235:genetics; D004958:Estradiol / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018519:Meristem / Q000235:genetics / Q000502:physiology; D008780:Methyltransferases / Q000235:genetics; D009154:Mutation; D018520:Plant Shoots / Q000235:genetics / Q000254:growth & development*; D018547:Plant Stems / Q000235:genetics; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D002352:Carrier Proteins; C496944:CycD3 protein, Arabidopsis; D016213:Cyclins; C585931:ERF BUD ENHANCER protein, Arabidopsis; C491109:MARD1 protein, Arabidopsis; D014157:Transcription Factors; D004958:Estradiol; D008780:Methyltransferases; C470731:DRM1 protein, Arabidopsis