title: Gender-related difference in bloodstain RNA ratio stored under uncontrolled room conditions for 28 days.
abstract: Bloodstain age is a parameter that can be used in crime scene investigations. Bloodstain age can be determined by measuring the 18S rRNA:β-actin mRNA ratio by Reverse Transcription-quantitative PCR (RT-qPCR). Since this ratio is a function of time, it can be used as an estimator of bloodstain age. However, it is important to validate the technique in a variety of scenarios before it can be applied. We investigated 18S rRNA:β-actin mRNA ratio in bloodstains from sixteen Chinese subjects in 28 days under uncontrolled room conditions. The ratio changed in a linear fashion. It was also found that the subjects' gender affected the relationship between time and the RNA ratio.
authors.affiliation: Department of Forensic Medicine, Norman Bethune College of Medical Sciences, Jilin University, Xinmin Street, Changchun 130021, PR China.
authors.forename: Baiyu
authors.initials: B
authors.lastname: Qi
authors.forename: Lingzhu
authors.initials: L
authors.lastname: Kong
authors.forename: Yingqiang
authors.initials: Y
authors.lastname: Lu
mesh_terms: D000199:Actins / Q000235:genetics*; D000328:Adult; D001801:Blood Stains; D005260:Female; D005554:Forensic Medicine / Q000379:methods; D006801:Humans; D016014:Linear Models; D008297:Male; D012333:RNA, Messenger / Q000097:blood*; D012337:RNA, Ribosomal, 18S / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D012737:Sex Factors; D013048:Specimen Handling; D013997:Time Factors; D055815:Young Adult
chemical_list: D000199:Actins; D012333:RNA, Messenger; D012337:RNA, Ribosomal, 18S