title: Pulsed laser deposition and in vitro characteristics of triphasic - HASi composition on titanium.
abstract: Pulsed laser deposition was used to deposit bioactive triphasic glass-ceramic composition (HASi) over titanium substrate using dense HASi target. Bioactive glass compositions are considered the most useful synthetic materials for immediate bone attachment because of its bioresorption, osteoconduction and osteointegration characteristics under in vivo conditions. The disadvantage of its brittleness associated with bioactive glass-ceramics has prompted its coating over metallic implants for the combination of duo mechanical and bioactive properties. The hard HASi target was able to undergo laser ablation under ambient gas pressure without bulk erosion of the target. Laser deposition was found to be efficient in depositing triphasic composition for immediate bone integration. The target and deposits were analyzed for the phase, composition and microstructural characteristics by means of X-ray diffraction, Fourier transform infrared spectroscopy, energy-dispersive X-ray analysis and scanning electron microscopy. Simultaneously, the adherent nature and mechanical behaviour of deposits were confirmed by scratch test and micro-indentation methods. Further, the in vitro dissolution and bioactivity were assessed by soaking in simulated body fluid followed by elemental analysis using inductively coupled plasma spectroscopy. The deposits were found to be cell-friendly, which was indicated by the phenomenology of stem cells under in vitro conditions.
authors.affiliation: 1Bioceramic Laboratory, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, Kerala, India.
authors.forename: Rajesh
authors.initials: R
authors.lastname: Palangadan
authors.forename: Anil
authors.initials: A
authors.lastname: Sukumaran
authors.forename: Francis B
authors.initials: FB
authors.lastname: Fernandez
authors.forename: Annie
authors.initials: A
authors.lastname: John
authors.forename: Harikrishna
authors.initials: H
authors.lastname: Varma
mesh_terms: D001672:Biocompatible Materials; D001826:Body Fluids; D001862:Bone Resorption; D001842:Bone and Bones / Q000502:physiology; D002478:Cells, Cultured; D002516:Ceramics; D005898:Glass; D066298:In Vitro Techniques; D007834:Lasers; D008855:Microscopy, Electron, Scanning; D016348:Osseointegration; D011208:Powders; D012995:Solubility; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D014025:Titanium / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D011208:Powders; D014025:Titanium
keywords: Calcium phosphate; bioactive coating; bioactive glass; laser deposition; titanium implant; triphasic