title: Effect of short-term storage on quality parameters, DNA integrity, and oxidative stress in Russian (Acipenser gueldenstaedtii) and Siberian (Acipenser baerii) sturgeon sperm.
abstract: The sperm of Russian sturgeon (A. gueldenstaedtii) and Siberian sturgeon (A. baerii) was used to evaluate the effects of short-term (liquid) storage on functional parameters (spermatozoa motility and velocity), DNA integrity and oxidative stress indices. Spermatozoa showed >50% motility during 6 days of storage with an average velocity of 133.12±15.4 to 87.9±11.23μm s(-1) in both species. No motile spermatozoa were recorded after nine days of storage. Analysis of Russian sturgeon sperm showed no significant differences in DNA damage expressed as percent tail DNA and Olive Tail Moment for first three days of storage. In Siberian sturgeon significant differences in DNA damage were detected after two days of storage. The level of oxidative stress indices (TBARS, CP) and antioxidant activity (SOD) increased significantly with storage time in both species. Results of this study can be utilized for successful reproduction management and cryopreservation protocols of these endangered species.
authors.affiliation: Faculty of Fisheries and Protection of Water, Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia, Vodnany, Czech Republic. a_shalutyna@mail.ru
authors.forename: Anna
authors.initials: A
authors.lastname: Shaliutina
authors.forename: Martin
authors.initials: M
authors.lastname: Hulak
authors.forename: Ievgenia
authors.initials: I
authors.lastname: Gazo
authors.forename: Pavla
authors.initials: P
authors.lastname: Linhartova
authors.forename: Otomar
authors.initials: O
authors.lastname: Linhart
mesh_terms: D000818:Animals; D020552:Comet Assay / Q000662:veterinary; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004249:DNA Damage; D056727:Endangered Species; D005399:Fishes / Q000235:genetics / Q000502:physiology*; D008297:Male; D018384:Oxidative Stress / Q000235:genetics / Q000502:physiology; D050050:Protein Carbonylation; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility / Q000502:physiology; D013094:Spermatozoa / Q000502:physiology*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D017392:Thiobarbituric Acid Reactive Substances; D004247:DNA