title: Differential expression and in vivo secretion of the antimicrobial peptides psoriasin (S100A7), RNase 7, human beta-defensin-2 and -3 in healthy human skin.
abstract: Antimicrobial peptides (AMP) are key players in the skin's defense system. Previous observations suggest a site- and age-dependent expression of individual AMP. We investigated the expression and secretion patterns of four important AMP in a representative collective of healthy human skin samples. Levels of psoriasin, RNase 7 and hBD-3 expression - assessed by immunohistochemistry - varied between different body localisations. Older individuals expressed hBD-2 more frequently. No gender-related expression was observed. The in vivo secretion of psoriasin, measured in skin washing fluids using ELISA, was related to body localisation and age, whereas RNase 7 secretion showed no significant differences regarding these variables. HBD-2 and -3 secretion could not be detected. Our findings suggest the usage of control samples matching localisation and approximate age (in the case of hBD-2) for comparative immunohistochemical analysis. To avoid bias through great interindividual differences, sufficient large collectives should be used for in vivo secretion analyses.
authors.forename: Maike
authors.initials: M
authors.lastname: Wittersheim
authors.forename: Jesko
authors.initials: J
authors.lastname: Cordes
authors.forename: Ulf
authors.initials: U
authors.lastname: Meyer-Hoffert
authors.forename: Jürgen
authors.initials: J
authors.lastname: Harder
authors.forename: Jürgen
authors.initials: J
authors.lastname: Hedderich
authors.forename: Regine
authors.initials: R
authors.lastname: Gläser
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D007113:Immunity, Innate / Q000502:physiology*; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D012260:Ribonucleases / Q000378:metabolism*; D000075023:S100 Calcium Binding Protein A7; D009418:S100 Proteins / Q000378:metabolism*; D012867:Skin / Q000276:immunology / Q000378:metabolism*; D055815:Young Adult; D023083:beta-Defensins / Q000378:metabolism*
chemical_list: C422950:DEFB103A protein, human; C106641:DEFB4A protein, human; D000075023:S100 Calcium Binding Protein A7; D009418:S100 Proteins; C089242:S100A7 protein, human; D023083:beta-Defensins; D012260:Ribonucleases; C470000:Ribonuclease 7