title: How does the 'ancient' asexual Philodina roseola (Rotifera: Bdelloidea) handle potential UVB-induced mutations?
abstract: Like other obligate asexuals, bdelloid rotifers are expected to suffer from degradation of their genomes through processes including the accumulation of deleterious mutations. However, sequence-based analyses in this regard remain inconclusive. Instead of looking for historical footprints of mutations in these ancient asexuals, we directly examined the susceptibility and ability to repair point mutations by the bdelloid Philodina roseola by inducing cyclobutane-pyrimidine dimers (CPDs) via exposure to UVB radiation (280-320 nm). For comparison, we performed analogous experiments with the facultative asexual monogonont rotifer Brachionus rubens. Different strategies were found for the two species. Philodina roseola appeared to shield itself from CPD induction through uncharacterized UV-absorbing compounds and, except for the genome reconstruction that occurs after desiccation, was largely unable to repair UVB-induced damage. By contrast, B. rubens was more susceptible to UVB irradiation, but could repair all induced damage in ~2 h. In addition, whereas UV irradiation had a significant negative impact on the reproductive output of P. roseola, and especially so after desiccation, that of B. rubens was unaffected. Although the strategy of P. roseola might suffice under natural conditions where UVB irradiation is less intense, the lack of any immediate CPD repair mechanisms in this species remains perplexing. It remains to be investigated how typical these results are for bdelloids as a group and therefore how reliant these animals are on desiccation-dependent genome repair to correct potential DNA damage given their obligate asexual lifestyle.
authors.affiliation: AG Systematics and Evolutionary Biology, IBU-Faculty V, Carl von Ossietzky Universität Oldenburg, Oldenburg, Germany. claus.fischer@uni-oldenburg.de
authors.forename: Claus
authors.initials: C
authors.lastname: Fischer
authors.forename: Wilko H
authors.initials: WH
authors.lastname: Ahlrichs
authors.forename: Anita G J
authors.initials: AG
authors.lastname: Buma
authors.forename: Willem H
authors.initials: WH
authors.lastname: van de Poll
authors.forename: Olaf R P
authors.initials: OR
authors.lastname: Bininda-Emonds
mesh_terms: D000818:Animals; D016296:Mutagenesis / Q000528:radiation effects*; D009154:Mutation / Q000235:genetics* / Q000528:radiation effects*; D011740:Pyrimidine Dimers / Q000378:metabolism; D012100:Reproduction, Asexual / Q000528:radiation effects*; D012403:Rotifera / Q000235:genetics* / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: D011740:Pyrimidine Dimers
keywords: UV damage; anhydrobiosis; asexual reproduction; bdelloid rotifer; cyclobutane-pyrimidine dimers; deleterious mutations; desiccation tolerance; genome degradation; genome repair