title: An artificial fusion protein between bone morphogenetic protein 2 and titanium-binding peptide is functional in vivo.
abstract: The purpose of this study was to investigate osteogenesis using an artificial fusion protein (AFP) composed of modified bone morphogenetic protein 2 (BMP-2) with a titanium (Ti)-binding peptide (TBP) motif on a Ti surface in vivo. In the in vivostudy, 5-μm thick Ti was coated with electron cyclotron resonance sputtering on a porous carbon scaffold which was then dipped in one of three different mixtures of collagen gel: (1) collagen gel only, (2) collagen gel with TBP, and (3) collagen gel with the AFP between BMP-2 and the TBP motif (AFP-TBP-BMP-2). These scaffolds were then implanted into rat abdominal muscles and were studied histologically at various times and the expression of several bone-related protein messenger RNAs (mRNAs) was also analyzed. The Ti-coated scaffold of the collagen gel with AFP-TBP-BMP-2 produced cartilage in the muscle and the expression of alkaline phosphatase, bone sialoprotein, and runt-related gene 2 mRNAs was significantly increased. These results suggest that the scaffold of the collagen gel with AFP-TBP-BMP-2 accelerates osteogenesis in vivo.
authors.affiliation: Department of Clinical Pathophysiology, Tokyo Dental College, Chiba, Japan.
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Yuasa
authors.forename: Eitoyo
authors.initials: E
authors.lastname: Kokubu
authors.forename: Katsutoshi
authors.initials: K
authors.lastname: Kokubun
authors.forename: Kenichi
authors.initials: K
authors.lastname: Matsuzaka
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Shiba
authors.forename: Kenji
authors.initials: K
authors.lastname: Kashiwagi
authors.forename: Takashi
authors.initials: T
authors.lastname: Inoue
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology*; D002356:Cartilage / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D020219:Chondrogenesis / Q000187:drug effects / Q000235:genetics; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D058574:Integrin-Binding Sialoprotein / Q000235:genetics / Q000378:metabolism; D010455:Peptides / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011993:Recombinant Fusion Proteins / Q000494:pharmacology*; D054457:Tissue Scaffolds; D014025:Titanium / Q000494:pharmacology*
chemical_list: D055396:Bone Morphogenetic Protein 2; D050659:Core Binding Factor Alpha 1 Subunit; D058574:Integrin-Binding Sialoprotein; D010455:Peptides; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D014025:Titanium
keywords: artificial protein; bone morphogenetic protein 2; in vivo; porous carbon; titanium-binding peptide