title: Label-free dsDNA-Cu NPs-based fluorescent probe for highly sensitive detection of L-histidine.
abstract: A new and facile strategy using double-stranded DNA-copper nanoparticles (dsDNA-Cu NPs) as fluorescence reporters for the highly sensitive and selective detection of l-histidine was demonstrated. In the dsDNA-Cu NPs-based sensing system, the fluorescence was quenched considerably upon the addition of l-histidine. Under the optimized experimental conditions, the probe exhibits excellent performance (e.g., a satisfactory detection limit of 5 μM and high specificity). Our in situ method requires no covalent attachment of DNA to a fluorophore, which could significantly reduce the cost and simplify the procedure for l-histidine detection. Moreover, the proposed sensing system could be applicable for the detection of target biomolecule in complex biological samples. These striking properties make it an attractive platform for the direct detection of l-histidine.
authors.affiliation: Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Hunan University, Changsha 410082, China.
authors.forename: Ya-Ru
authors.initials: YR
authors.lastname: Liu
authors.forename: Rong
authors.initials: R
authors.lastname: Hu
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.forename: Xiao-Bing
authors.initials: XB
authors.lastname: Zhang
authors.forename: Weihong
authors.initials: W
authors.lastname: Tan
authors.forename: Guo-Li
authors.initials: GL
authors.lastname: Shen
authors.forename: Ru-Qin
authors.initials: RQ
authors.lastname: Yu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D003300:Copper / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006639:Histidine / Q000032:analysis*; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D005456:Fluorescent Dyes; D006639:Histidine; D003300:Copper; D004247:DNA