title: Genome-scale genetic engineering in Escherichia coli.
abstract: Genome engineering has been developed to create useful strains for biological studies and industrial uses. However, a continuous challenge remained in the field: technical limitations in high-throughput screening and precise manipulation of strains. Today, technical improvements have made genome engineering more rapid and efficient. This review introduces recent advances in genome engineering technologies applied to Escherichia coli as well as multiplex automated genome engineering (MAGE), a recent technique proposed as a powerful toolkit due to its straightforward process, rapid experimental procedures, and highly efficient properties.
authors.affiliation: Department of Chemistry, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-749, Republic of Korea.
authors.forename: Jaehwan
authors.initials: J
authors.lastname: Jeong
authors.forename: Namjin
authors.initials: N
authors.lastname: Cho
authors.forename: Daehee
authors.initials: D
authors.lastname: Jung
authors.forename: Duhee
authors.initials: D
authors.lastname: Bang
mesh_terms: D001709:Biotechnology / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics*; D005818:Genetic Engineering; D016680:Genome, Bacterial; D057166:High-Throughput Screening Assays; D011995:Recombination, Genetic
keywords: Genome engineering; Multiplex automated genome engineering; λ Red recombination