title: Role of mitochondrial fission in neuronal injury in pilocarpine-induced epileptic rats.
abstract: Mitochondrial fission has been reported to be involved in oxidative stress, apoptosis and many neurological diseases. However, the role of mitochondrial fission in seizures, which could induce oxidative stress and neuronal loss, remains unknown. In this study, we used pilocarpine to elicit seizures in rats. Meanwhile, we used mitochondrial division inhibitor 1 (mdivi-1), a selective inhibitor of mitochondrial fission protein dynamin-related protein1 (Drp1), to suppress mitochondrial fission in epileptic model of rats in vivo. We found that mitochondrial fission was increased after seizures and the inhibition of mitochondrial fission by mdivi-1 significantly attenuated oxidative stress and reduced neuronal loss after seizures, shown by the decreased 8-hydroxy deoxyguanosine (8-oHdG) content, the increased superoxide dismutase (SOD) activity, the reduced expression of cytochrome c and caspase3 and the increased surviving neurons in the hippocampus. These results indicated that mitochondrial fission is up-regulated after seizures and the inhibition of mitochondrial fission is protective against neuronal injury in seizures, the underlying mechanism may be through the mitochondria/reactive oxygen species (ROS)/cytochrome c pathway.
authors.affiliation: Department of Neurology, Qilu Hospital of Shandong University, 107#, Wenhua Xi Road, Jinan 250012, China.
authors.forename: X
authors.initials: X
authors.lastname: Qiu
authors.forename: L
authors.initials: L
authors.lastname: Cao
authors.forename: X
authors.initials: X
authors.lastname: Yang
authors.forename: X
authors.initials: X
authors.lastname: Zhao
authors.forename: X
authors.initials: X
authors.lastname: Liu
authors.forename: Y
authors.initials: Y
authors.lastname: Han
authors.forename: Y
authors.initials: Y
authors.lastname: Xue
authors.forename: H
authors.initials: H
authors.lastname: Jiang
authors.forename: Z
authors.initials: Z
authors.lastname: Chi
mesh_terms: D000818:Animals; D004827:Epilepsy / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D008297:Male; D063154:Mitochondrial Dynamics / Q000502:physiology*; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D010862:Pilocarpine / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010862:Pilocarpine