title: [Genetic transformation of Pinellia ternata with Agrobacterium tumefaciens-mediated sHSP genes].
abstract: OBJECTIVE
  To establish an efficient genetic transformation system of Pinellia ternata.
  
  
  METHODS
  With petioles from test-tube seedlings of P. ternata as explants, Agrobacterium tumefaciens mediation method was adopted to explore the effect of phenolic substances, A. tumefaciens's concentration, infection time, pre-incubation time and co-cultivation time on genetic transformation efficiency of P. ternata.
  
  
  CONCLUSIONS
  The genetic transformation efficiency could be effectively enhanced by infecting in A. tumefaciens culture containing AS 40 mg x L(-1) for 15 min for three days. The petioles were put into the differentiation medium containing 150 mg x L(-1) Kan and 350 mg x L(-1) Carb to screening and cultivation. After around 30 days, microtubers could be observed at both sides of the petioles. Gus staining and PCR verification on the regenerated plants showed that the exogenous gene sHSP had been integrated into genome of P. ternata.
authors.affiliation: Anhui Key Laboratory of Plant Resources and Biology, School of Life science, Huaibei Normal University, Huaibei 235000, China.
authors.forename: Zhao-Yang
authors.initials: ZY
authors.lastname: Guo
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Cui
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Xue
authors.forename: Yan-Fang
authors.initials: YF
authors.lastname: Zhu
authors.forename: Ai-Min
authors.initials: AM
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Sheng
authors.forename: Jing-Tong
authors.initials: JT
authors.lastname: Teng
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D050888:Heat-Shock Proteins, Small / Q000235:genetics*; D030021:Pinellia / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D046509:Tissue Culture Techniques / Q000379:methods; D014170:Transformation, Genetic
chemical_list: D018744:DNA, Plant; D050888:Heat-Shock Proteins, Small; D005966:Glucuronidase