title: Immunomodulating pectic polysaccharides from waste rose petals of Rosa damascena Mill.
abstract: A water-soluble polysaccharide (RP-1) was obtained from distilled rose petals of Rosa damascena Mill. as an attempt for valorization of the waste. RP-1 showed in vitro intestinal immune system modulating activity through Peyer's patch cells and IL-6 producing activity from macrophages. RP-1 lost most of its immunomodulating activity by degradation of the carbohydrate moiety with periodate. RP-1 was fractionated by anion-exchange and gel filtration chromatography and some of the fractions showed significant intestinal immune system modulating activity. The active fractions were suggested to be pectic polysaccharides and type II arabino-3,6-galactan from the component sugar analyses and the reactivity with Yariv antigen. When some active fractions were digested with endo α-d-(1→4)-polygalacturonase, highest molecular weight fragments which were considered as rhamnogalacturonan I, showed potent immunomodulating activities. To our knowledge, this is a first report which explores the possibility for utilization of waste rose petals as a source of immunomodulating pectic polysaccharides.
authors.affiliation: Kitasato Institute for Life Sciences & Graduate School of Infection Control Science, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan. antons@uni-plovdiv.net
authors.forename: Anton
authors.initials: A
authors.lastname: Slavov
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kiyohara
authors.forename: Haruki
authors.initials: H
authors.lastname: Yamada
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002852:Chromatography, Ion Exchange; D005260:Female; D005685:Galactans / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D056747:Immunomodulation / Q000187:drug effects; D007220:Industrial Waste; D015850:Interleukin-6 / Q000096:biosynthesis / Q000276:immunology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D010368:Pectins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010504:Periodic Acid / Q000737:chemistry; D010581:Peyer's Patches / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D011096:Polygalacturonase / Q000737:chemistry; D027826:Rosa / Q000737:chemistry*
chemical_list: D005685:Galactans; D007220:Industrial Waste; D015850:Interleukin-6; C042491:rhamnogalacturonan I; D010504:Periodic Acid; D010368:Pectins; C009288:metaperiodate; D011096:Polygalacturonase