title: Quantitative determination of the Fusarium mycotoxins beauvericin, enniatin A, A1, B and B1 in pig plasma using high performance liquid chromatography-tandem mass spectrometry.
abstract: A sensitive and reliable method was developed for the identification and quantification of beauvericin, enniatin A, A1, B and B1 in pig plasma using liquid chromatography combined with heated electrospray ionization tandem mass spectrometry. Sample clean-up consisted of a deproteinization step using acetonitrile, followed by evaporation of the supernatant and resuspension of the dry residue in acetonitrile/water (80/20, v/v). The method was in-house validated: matrix-matched calibration graphs were prepared for all compounds and correlation and goodness-of-fit coefficients ranged between 0.9980 and 0.9995 and between 5.2% and 11.3%, respectively. The within- and between-run precision and accuracy were evaluated and the results fell within the ranges specified. The limits of quantification were 0.1 ng/mL for enniatin A and A1 and 0.2 ng/mL for beauvericin, enniatin B and B1, whereas limits of detection were ≤ 10 pg/mL for all analytes. The method has been applied for the analysis of real plasma samples from one pig that received an oral bolus (0.05 mg/kg BW) of the investigated mycotoxins. At the applied dosage, the results indicated the suitability of the method for use in toxicokinetic studies with enniatins.
authors.affiliation: Department of Pharmacology, Toxicology and Biochemistry, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium. mathias.devreese@ugent.be
authors.forename: Mathias
authors.initials: M
authors.lastname: Devreese
authors.forename: Siegrid
authors.initials: S
authors.lastname: De Baere
authors.forename: Patrick
authors.initials: P
authors.lastname: De Backer
authors.forename: Siska
authors.initials: S
authors.lastname: Croubels
mesh_terms: D000097:Acetonitriles; D000818:Animals; D001798:Blood Proteins / Q000737:chemistry; D002138:Calibration; D011232:Chemical Precipitation; D002851:Chromatography, High Pressure Liquid; D047630:Depsipeptides / Q000097:blood / Q000302:isolation & purification*; D005670:Fusarium / Q000737:chemistry; D057230:Limit of Detection; D009183:Mycotoxins / Q000097:blood / Q000302:isolation & purification*; D021241:Spectrometry, Mass, Electrospray Ionization; D013552:Swine; D053719:Tandem Mass Spectrometry
chemical_list: D000097:Acetonitriles; D001798:Blood Proteins; D047630:Depsipeptides; D009183:Mycotoxins; C100264:enniatins; C004456:beauvericin; C032159:acetonitrile