title: Interleukin-10 controls human peripheral PMN activation triggered by lipopolysaccharide.
abstract: Large amounts of anti-inflammatory mediators, such as interleukin (IL)-10, are produced and found early in the course of sepsis. We explore the role of IL-10 on neutrophil (PMN) activation/function using an in vitro model. Isolated human PMN were pre-incubated with lipopolysaccharide (LPS) and/or IL-10 for 18h. Subsequently, a second LPS exposure was performed and CD11b and CD66b up-regulation, and the reactive oxygen species (ROS) generation were measured 2h later. We found that IL-10 prevented PMN activation and the secretion of TNF-α and IL-8 induced by the first LPS contact. In the absence of IL-10, a second LPS exposure induced additive effects that were prevented by IL-10. Only ROS generation was highly affected by the blockade of PMN-secreted TNF-α or IL-8. Additionally, IL-10 prevented other possible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrine activating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposure.
authors.affiliation: Laboratorio de Fisiologia de los Procesos Inflamatorios, Instituto de Medicina Experimental IMEX-CONICET/Academia Nacional de Medicina de Buenos Aires, Argentina.
authors.forename: Daiana
authors.initials: D
authors.lastname: Martire-Greco
authors.forename: Nahuel
authors.initials: N
authors.lastname: Rodriguez-Rodrigues
authors.forename: Verónica I
authors.initials: VI
authors.lastname: Landoni
authors.forename: Barbara
authors.initials: B
authors.lastname: Rearte
authors.forename: Martín A
authors.initials: MA
authors.lastname: Isturiz
authors.forename: Gabriela C
authors.initials: GC
authors.lastname: Fernández
mesh_terms: D019898:Autocrine Communication / Q000187:drug effects; D015550:Cell Degranulation / Q000187:drug effects; D002469:Cell Separation; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism*; D016209:Interleukin-8 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D018375:Neutrophil Activation / Q000187:drug effects*; D009504:Neutrophils / Q000187:drug effects* / Q000378:metabolism* / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism; D013682:Teichoic Acids / Q000494:pharmacology
chemical_list: C508609:IL10 protein, human; D016209:Interleukin-8; D008070:Lipopolysaccharides; D017382:Reactive Oxygen Species; D013682:Teichoic Acids; D016753:Interleukin-10; C009900:lipoteichoic acid