title: Simultaneous determination of five flavonoids during the growth of Fructus Sophorae by capillary electrophoresis.
abstract: A method of simultaneous determination of five flavonoids during the growth of Fructus Sophorae by β-cyclodextrin (β-CD-) modified capillary zone electrophoresis was developed. The effects of various parameters such as buffer concentration, pH, applied voltage, and β-CD concentrations were investigated. After a series of optimization processes, the determination of five flavonoids in Fructus Sophorae was successfully achieved in 20 mmol/L borax buffer (pH 9.5), 25 kV applied voltage, and 8 mmol/L β-CD. The linearity, detection limits, repeatability, and recovery were satisfactory. Thus, the proposed β-CD-modified capillary zone electrophoresis method was satisfactorily used to analyze Fructus Sophorae samples. The results can be useful for the quality control and medicinal resource development of Fructus Sophorae.
authors.affiliation: Department of Chemistry, Dezhou University, Dezhou, 253023, China, 2003liyumei@sina.com.
authors.forename: Yu-Mei
authors.initials: YM
authors.lastname: Li
mesh_terms: D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D019075:Electrophoresis, Capillary; D005419:Flavonoids / Q000032:analysis*; D005638:Fruit / Q000737:chemistry; D015394:Molecular Structure; D029909:Sophora / Q000737:chemistry*; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D047392:beta-Cyclodextrins; C031215:betadex