title: Influence of low-power laser illumination on pro-inflammatory response in human endothelial cells stimulated with interleukin-1a.
abstract: BACKGROUND
  Regarding recent findings on the proven influence of laser light on healing and endothelial regeneration processes, this study was conducted in order to examine the influence of low-power laser illumination on the endothelial inflammatory response.
  
  
  METHODS
  Human umbilical vein endothelial cells (HUVECs) isolated from umbilical cord, cultured in standard conditions, were harvested and passaged in 24-well plates. Laser influence on HUVEC inflammatory response was measured by stimulating them with Interleukin-1β (IL-1β) followed by laser light illumination. A 808-nm wave length laser diode and light energy doses of 1.5 and 4.5 J/cm(2) were used (50 mW for 90 and 270 s respectively). The response was measured by assessing Cluster of differentiation 54 (CD54), Cluster of differentiation 62E (CD62E), Monocyte chemotactic protein-1 (MCP-1) expression and von Willebrand factor (vWF) release, at 6 and 24 h after stimulation. The MCP-1 and vWF activity in cell supernatants was measured with an enzyme-linked immunosorbent assay (ELISA) kit. Cytofluorometry was used to assess CD54 and CD62E expression.
  
  
  RESULTS
  MCP-1 concentration in supernatants from HUVECs was significantly lower 6 h after 4.5 J/cm(2) stimulation compared to IL-1β-stimulated cells. No changes in MCP-1 levels after IL-1β stimulation plus 1.5 J/cm(2) illumination, compared to IL-1β stimulated HUVECs were noted. IL-1β stimulation significantly enhanced the concentration of vWF and the expression of CD54 and CD62E. Both energies of laser light illumination inhibited the IL-1β-induced increase of CD54 and CD62E concentration. vWF activity after illumination was comparable to that of unstimulated cells. There were no significant differences in the viable cell count between the groups tested.
  
  
  CONCLUSIONS
  Low-power laser illumination diminishes the pro-inflammatory and pro-coagulant activity of IL-1β-stimulated HUVECs.
authors.affiliation: Cardiology Department, Medical University of Wroclaw, Wroclaw ul.Borowska 213, 50-556 Wroclaw, Poland. mprot@poczta.onet.pl
authors.forename: Marcin
authors.initials: M
authors.lastname: Protasiewicz
authors.forename: Wiktor
authors.initials: W
authors.lastname: Kuliczkowski
authors.forename: Anna Kamila
authors.initials: AK
authors.lastname: Woznicka
authors.forename: Pawel
authors.initials: P
authors.lastname: Szymkiewicz
authors.forename: Arkadiusz
authors.initials: A
authors.lastname: Derkacz
authors.forename: Mysiak
authors.initials: M
authors.lastname: Andrzej
authors.forename: Piotr
authors.initials: P
authors.lastname: Dziegiel
mesh_terms: D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D018932:Chemokine CCL2 / Q000378:metabolism; D019040:E-Selectin / Q000378:metabolism; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D007249:Inflammation / Q000378:metabolism*; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D053582:Interleukin-1alpha / Q000494:pharmacology*; D007834:Lasers; D014841:von Willebrand Factor / Q000378:metabolism
chemical_list: D018932:Chemokine CCL2; D019040:E-Selectin; D053582:Interleukin-1alpha; D014841:von Willebrand Factor; D018799:Intercellular Adhesion Molecule-1