title: HPLC-MS-Method for A-Trichothecenes.
abstract: Gaschromatography with electron capture detection belongs to the most important methods for analysing the A-trichothecenes T-2 toxin, HT-2 toxin and diacetoxyscirpenol. The need of a derivatisation for these mycotoxins prior detection is the main disadvantage of this method. In this paper a sensitive high performance liquid chromatography method to analyse the A-trichothecenes in grain is described. The clean up was performed with multifunctional columns (MycoSep(TM), Romer Labs, Inc., MO). The detection limits of these methods are 3.5 μg/kg for T-2 toxin, 6.1 μg/kg for HT-2 toxin and 4.1 μg/kg for diacetoxyscirpenol. Limits of quantification were 12.7 μg/kg for T-2, 21.9 μg/kg for HT-2 and 14.8 μg/kg for DAS, respectively recovery rates were about 100% for each compound.
authors.affiliation: Biomin G.T.I. GmbH, Industriestr. 21, A-3130, Herzogenburg.
authors.forename: N
authors.initials: N
authors.lastname: Thimm
authors.forename: J
authors.initials: J
authors.lastname: Handl
authors.forename: M
authors.initials: M
authors.lastname: Binder