title: Potential implication of N-acetylcysteine detoxification on adhesive endodontics.
abstract: Methacrylate resin-based dentin adhesives and root canal sealers used for bonding of filling materials inside the root canal system are cytotoxic and result in reduction in cell proliferation to a variable extent. An in vitro study to examine the detoxifying effects of N-acetylcysteine (NAC) on four commercial adhesive systems on adhesive-induced cytotoxicity and cell survival was conducted so that the use of methacrylate resin-based materials for filling root canals could be optimized. The finding that NAC co-treatment protected the cells from adhesive-induced toxicity by increasing cellular proliferation, attenuating cell cycle arrest, and reducing cell death suggests the null hypothesis that NAC has no effect on dentin adhesive-induced cell death and cell cycle arrest should be rejected.
authors.affiliation: State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, PR China.
authors.forename: Huang
authors.initials: H
authors.lastname: Xueqing
authors.forename: Zuo
authors.initials: Z
authors.lastname: Chenqi
authors.forename: Du
authors.initials: D
authors.lastname: Xijin
authors.forename: Chen
authors.initials: C
authors.lastname: Huabing
authors.forename: Huang
authors.initials: H
authors.lastname: Cui
authors.forename: Franklin
authors.initials: F
authors.lastname: Tay
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival; D003188:Composite Resins / Q000633:toxicity*; D003738:Dental Cements / Q000633:toxicity*; D017220:Dentin-Bonding Agents / Q000633:toxicity*; D005347:Fibroblasts / Q000187:drug effects*; D066298:In Vitro Techniques; D008689:Methacrylates / Q000633:toxicity*; D011109:Polymethacrylic Acids / Q000633:toxicity*; D011137:Polystyrenes
chemical_list: C554641:Adper single bond 2; D003188:Composite Resins; D003738:Dental Cements; D017220:Dentin-Bonding Agents; C498353:G-Bond; D008689:Methacrylates; D011109:Polymethacrylic Acids; D011137:Polystyrenes; C425843:Prime and Bond NT; C584661:easy bond; D000111:Acetylcysteine