title: A statistical approach for optimization of polyhydroxybutyrate production by marine Bacillus subtilis MSBN17.
abstract: The important biological macromolecule polyhydroxybutyrate (PHB) producing Bacillus subtilis was isolated from the marine sponge Callyspongia diffusa and identified by means of 16S rRNA analysis. The central composite design (CCD) was used to optimize the PHB production using cheap raw materials such as pulp industry waste (PIW), tamarind kernel powder (TKP), palm jaggery (PJ) and green gram flour (GGF). The extracted polymer was characterized by (1)H NMR analysis. The PIW was fed at three different intervals and the maximum production of PHB (19.08g/L) was attained after a period of 40h of incubation of B. subtilis. Dissolved oxygen, sodium chloride and nitrogen source were found to be the critical control factors that affected the PHB polymer production. The present investigation demonstrates an inexpensive model of producing PHB green thermoplastics in vitro for biomedical applications.
authors.affiliation: Department of Bioinformatics, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India.
authors.forename: G
authors.initials: G
authors.lastname: Sathiyanarayanan
authors.forename: G
authors.initials: G
authors.lastname: Saibaba
authors.forename: G
authors.initials: G
authors.lastname: Seghal Kiran
authors.forename: Joseph
authors.initials: J
authors.lastname: Selvin
mesh_terms: D000818:Animals; D001412:Bacillus subtilis / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D019149:Bioreactors; D047128:Callyspongia / Q000382:microbiology; D003470:Culture Media; D005163:Factor Analysis, Statistical; D005285:Fermentation; D055772:Green Chemistry Technology; D006885:Hydroxybutyrates / Q000378:metabolism*; D007220:Industrial Waste; D009584:Nitrogen / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012965:Sodium Chloride / Q000378:metabolism
chemical_list: D003470:Culture Media; D006885:Hydroxybutyrates; D007220:Industrial Waste; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; D009584:Nitrogen; D010100:Oxygen