title: Bordetella holmesii: initial genomic analysis of an emerging opportunist.
abstract: Bordetella holmesii is an emerging opportunistic pathogen that causes respiratory disease in healthy individuals and invasive infections among patients lacking splenic function. We used 16S rRNA gene analysis to confirm B. holmesii as the cause of bacteremia in a child with sickle cell disease. Semiconductor-based draft genome sequencing provided insight into B. holmesii phylogeny and potential virulence mechanisms and also identified a toluene-4-monoxygenase locus unique among bordetellae.
authors.affiliation: Department of Pediatrics, Columbia University, New York, NY, USA.
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Planet
authors.forename: Apurva
authors.initials: A
authors.lastname: Narechania
authors.forename: Saul R
authors.initials: SR
authors.lastname: Hymes
authors.forename: Christina
authors.initials: C
authors.lastname: Gagliardo
authors.forename: Richard C
authors.initials: RC
authors.lastname: Huard
authors.forename: Susan
authors.initials: S
authors.lastname: Whittier
authors.forename: Phyllis
authors.initials: P
authors.lastname: Della-Latta
authors.forename: Adam J
authors.initials: AJ
authors.lastname: Ratner
mesh_terms: D000755:Anemia, Sickle Cell / Q000150:complications* / Q000235:genetics; D016470:Bacteremia / Q000382:microbiology*; D001884:Bordetella / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D001885:Bordetella Infections / Q000150:complications / Q000382:microbiology*; D002648:Child; D005260:Female; D016680:Genome, Bacterial; D023281:Genomics / Q000379:methods*; D006801:Humans; D009894:Opportunistic Infections / Q000150:complications / Q000382:microbiology*; D010105:Oxygenases / Q000235:genetics; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D012336:RNA, Ribosomal, 16S; D010105:Oxygenases; C068610:toluene-4-monooxygenase