title: Identification, expression and subcellular localization of ESRG.
abstract: ESRG (embryonic stem cell related gene, also known as HESRG), is a novel human gene first cloned and identified by our group with microarray analysis. Interestingly, it is expressed specifically in undifferentiated human embryonic stem cells (hESCs), while its expression pattern and its role in hESCs remain unclear. Here, full-length 3151nt ESRG cDNA was further identified by RNA ligase mediated rapid amplification of cDNA ends (RLM-RACE) technique. Meanwhile, an alternatively splicing ESRG transcript (ESRG-B) of 2837nt in length was also found. Surprisingly, bioinformatics analyses showed that the open reading frames (ORFs) of ESRG and ESRG-B were identical. Both of them consist of 669nt and encode a 222aa protein with a predicted molecular size of 24 kDa. The ESRG protein was located in the nuclei of hESCs as demonstrated by immunocytochemical staining and Western blotting using ESRG specific antibody generated by us. In contrast, ESRG located in the cytoplasm of COS7 cells when it was forced to be expressed in these cells by gene transfection strategy, suggesting there may be some special proteins present only in hESCs which can help ESRG protein transport into the nuclei of hESCs. By spatial expression analysis, we further discovered that ESRG only expressed in the ovary tissue and hESCs instead of other tissues or cell lines. Our current data provide us with an important basis for conducting further studies on the functions and regulatory mechanisms underlying the role of ESRG in hESCs.
authors.affiliation: Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, Key Laboratory for Carcinogenesis of Chinese Ministry of Health, Changsha, Hunan 410078, PR China.
authors.forename: Guifei
authors.initials: G
authors.lastname: Li
authors.forename: Caiping
authors.initials: C
authors.lastname: Ren
authors.forename: Jia
authors.initials: J
authors.lastname: Shi
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.forename: Xiangling
authors.initials: X
authors.lastname: Feng
authors.forename: Weidong
authors.initials: W
authors.lastname: Liu
authors.forename: Bin
authors.initials: B
authors.lastname: Zhu
authors.forename: Chang
authors.initials: C
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Kaitai
authors.initials: K
authors.lastname: Yao
authors.forename: Xingjun
authors.initials: X
authors.lastname: Jiang
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D019556:COS Cells; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism*; D002522:Chlorocebus aethiops; D003593:Cytoplasm / Q000378:metabolism; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005333:Fetus / Q000378:metabolism; D020869:Gene Expression Profiling; D006801:Humans; D051379:Mice; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D010053:Ovary / Q000196:embryology / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D011506:Proteins / Q000235:genetics* / Q000378:metabolism*; D062085:RNA, Long Noncoding; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C524884:ESRG lncRNA, human; D020033:Protein Isoforms; D011506:Proteins; D062085:RNA, Long Noncoding