title: [Proteome analysis on interaction between Anoectochilus roxburghii and Mycorrhizal fungus].
abstract: OBJECTIVE
  To study the mechanism of plant growing promoted by Mycorrhizal fungus through the difference of proteomes.
  
  
  METHODS
  The differential proteomes between uninoculated and inoculated endophytic fungi, Epulorhiza sp. on Anoectochilus roxburghii were analyzed by two-dimensional gel electrophoresis and MALDI-TOF/TOF mass spectrum.
  
  
  CONCLUSIONS
  Twenty-seven protein spots were analyzed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Twenty-two candidate proteins were identified by database comparisons. The function of these proteins mostly involved in signal transduction, metabolic regulation, as well as photosynthesis and substance metabolism. The results indicate that the regulator control system of plant is influenced by fungi action, and the positive regulation improves substance metabolism and photosynthesis, which results in strong plant and higher resistance. It is also deduced that silent genes may exist in endosymbiosis plants.
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Gao
authors.forename: Shun-Xing
authors.initials: SX
authors.lastname: Guo
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
authors.forename: Li-Chun
authors.initials: LC
authors.lastname: Zhang
mesh_terms: D001487:Basidiomycota / Q000502:physiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D054884:Host-Pathogen Interactions; D038821:Mycorrhizae / Q000502:physiology*; D029595:Orchidaceae / Q000254:growth & development / Q000378:metabolism* / Q000382:microbiology*; D010940:Plant Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D010940:Plant Proteins; D020543:Proteome