title: The relationship between insulin resistance and CpG island methylation of LMNA gene in polycystic ovary syndrome.
abstract: We sought to investigate the relationship between the changes of CpG island methylation status of LMNA gene and insulin resistance in polycystic ovary syndrome (PCOS) patients. The genome-wide methylation microarray screening was done in three PCOS cases of insulin resistance and one case of a normal woman. The PCOS insulin resistance-related genes were identified as indicated by the results of gene chip screening. Then, 24 cases of insulin-resistant PCOS patients and 24 cases of normal individuals were studied to identify the effects of the candidate genes using genome-wide study of DNA from the peripheral blood analyzed by MassARRAY(®)EpiTYPER™ DNA methylation analysis technique. We found that the methylation status of CpG island in the promoter area of LMNA gene was changed. The 20 CG sites in CpG island of LMNA gene were examined using case control experiment among which 12 CpG sites differed significantly (P < 0.05) between two groups while the remaining eight CpG sites differed non-significantly. We, therefore, concluded that the changes in the hypermethylation status of CpG island of LMNA gene were related to the insulin resistance in PCOS patients, indicating that this gene may be involved in the regulation of PCOS-associated insulin resistance.
authors.affiliation: Obstetric and Gynecology Hospital of Fudan University, Shanghai, China, wangting_03@163.com.
authors.forename: Wang
authors.initials: W
authors.lastname: Ting
authors.forename: Qian
authors.initials: Q
authors.lastname: Yanyan
authors.forename: Huang
authors.initials: H
authors.lastname: Jian
authors.forename: Hua
authors.initials: H
authors.lastname: Keqin
authors.forename: Ma
authors.initials: M
authors.lastname: Duan
mesh_terms: D000328:Adult; D018899:CpG Islands; D004247:DNA / Q000378:metabolism; D019175:DNA Methylation; D005260:Female; D006801:Humans; D007333:Insulin Resistance; D034904:Lamin Type A / Q000235:genetics* / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D011085:Polycystic Ovary Syndrome / Q000235:genetics* / Q000503:physiopathology*; D024661:Potassium Channels, Inwardly Rectifying / Q000235:genetics; D012269:Ribosomal Proteins / Q000235:genetics; D000099161:Kcnj11 Channel
chemical_list: D004247:DNA; D034904:Lamin Type A; D024661:Potassium Channels, Inwardly Rectifying; D012269:Ribosomal Proteins; D000099161:Kcnj11 Channel; C494847:LMNA protein, human