title: Dual effects of peroxisome proliferator-activated receptor γ on embryonic stem cell self-renewal in presence and absence of leukemia inhibitory factor.
abstract: The aim of this study was to evaluate the influence of peroxisome proliferator-activated receptor γ (PPARγ) on self-renewal of mouse embryonic stem cells (mESCs) in the presence and absence of leukemia inhibitory factor (LIF). We demonstrated that in the presence of LIF, the activation of PPARγ by Rosiglitazone led to an increased proliferation of mESCs whereas PPARγ antagonist (GW9662) reversed this effect. Additionally, upon PPARγ activation, LIF increased PPARγ expression and resulted in the degradation of suppressor of cytokine signaling 3 (SOCS3), an important negative regulator of LIF/signal transducers and activators of transcription 3 (STAT3)-pathway. In the absence of LIF, Rosiglitazone decreased proliferation of mESCs. In this state, our results showed that extracellular signal-regulated kinase (ERK) proteins were activated and resulted in the suppression of Nanog expression, an important pluripotency determinant, whereas it did not affect Oct4 expression. These results suggest that the pivotal role of PPARγ on mESC self-renewal depends on the presence and absence of LIF.
authors.affiliation: Department of Cellular Biotechnology at Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Peymani
authors.forename: Ali
authors.initials: A
authors.lastname: Ghoochani
authors.forename: Kamran
authors.initials: K
authors.lastname: Ghaedi
authors.forename: Fereshteh
authors.initials: F
authors.lastname: Karamali
authors.forename: Khadijeh
authors.initials: K
authors.lastname: Karbalaie
authors.forename: Abbas
authors.initials: A
authors.lastname: Kiani-Esfahani
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Rabiee
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Nasr-Esfahani
authors.forename: Hossein
authors.initials: H
authors.lastname: Baharvand
mesh_terms: D000813:Anilides / Q000494:pharmacology; D000818:Animals; D002454:Cell Differentiation; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000502:physiology*; D018398:Homeodomain Proteins / Q000378:metabolism; D053605:Leukemia Inhibitory Factor / Q000502:physiology*; D051379:Mice; D000071317:Nanog Homeobox Protein; D047495:PPAR gamma / Q000819:agonists / Q000037:antagonists & inhibitors / Q000502:physiology*; D021381:Protein Transport / Q000187:drug effects; D000077154:Rosiglitazone; D050796:STAT3 Transcription Factor / Q000378:metabolism; D015398:Signal Transduction; D045162:Thiazolidinediones / Q000494:pharmacology
chemical_list: C457499:2-chloro-5-nitrobenzanilide; D000813:Anilides; D018398:Homeodomain Proteins; D053605:Leukemia Inhibitory Factor; D000071317:Nanog Homeobox Protein; C475540:Nanog protein, mouse; D047495:PPAR gamma; D050796:STAT3 Transcription Factor; C494087:Stat3 protein, mouse; D045162:Thiazolidinediones; D000077154:Rosiglitazone