title: Positive association between plasma levels of oxidized low-density lipoprotein and myeloperoxidase after hemodialysis in patients with diabetic end-stage renal disease.
abstract: End-stage renal disease (ESRD) patients undergoing hemodialysis (HD) have a high prevalence of cardiovascular events. Low-density lipoprotein (LDL) in dialysis patients has been shown to be susceptible to in vitro peroxidation; therefore, oxidized-LDL (ox-LDL) could be generated in these patients. Moreover, myeloperoxidase (MPO) released from activated neutrophils may play a role in the induction of LDL oxidation. The purpose of this study was to investigate the relationship between plasma ox-LDL levels, plasma MPO levels, and serum high-sensitivity C-reactive protein (hs-CRP) levels during initial HD in patients with diabetic ESRD. Patients (n=28) had serial venous blood samples drawn before and after HD at the initial, second, and third sessions. Plasma ox-LDL levels were measured using a specific monoclonal antibody (DLH3), and plasma MPO levels were measured using an enzyme-linked immunosorbent assay kit. Plasma ox-LDL levels and MPO levels after a single HD session increased significantly (ox-LDL, P<0.005; MPO, P<0.0001) compared with levels before that HD session. However, the increase was transient since the levels returned to pre-HD session levels. Additionally, plasma MPO levels showed a positive correlation with plasma ox-LDL levels during HD (R=0.62, P=0.0029). No significant change was observed in serum hs-CRP levels before and after each HD session. This study demonstrates that plasma MPO levels are directly associated with plasma ox-LDL levels in diabetic ESRD patients during initial HD. These findings suggest a pivotal role for MPO and ox-LDL in the progression and acceleration of atherosclerosis in patients undergoing HD.
authors.affiliation: Department of Pathology, Osaka City University Graduate School of Medicine, Tokyo, Japan.
authors.forename: Chizuko
authors.initials: C
authors.lastname: Kitabayashi
authors.forename: Takahiko
authors.initials: T
authors.lastname: Naruko
authors.forename: Kenichi
authors.initials: K
authors.lastname: Sugioka
authors.forename: Kei
authors.initials: K
authors.lastname: Yunoki
authors.forename: Masashi
authors.initials: M
authors.lastname: Nakagawa
authors.forename: Mayumi
authors.initials: M
authors.lastname: Inaba
authors.forename: Masahiko
authors.initials: M
authors.lastname: Ohsawa
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Konishi
authors.forename: Masahito
authors.initials: M
authors.lastname: Imanishi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Inoue
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Itabe
authors.forename: Minoru
authors.initials: M
authors.lastname: Yoshiyama
authors.forename: Kazuo
authors.initials: K
authors.lastname: Haze
authors.forename: Anton E
authors.initials: AE
authors.lastname: Becker
authors.forename: Makiko
authors.initials: M
authors.lastname: Ueda
mesh_terms: D050197:Atherosclerosis / Q000097:blood*; D048909:Diabetes Complications / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000628:therapy*; D008077:Lipoproteins, LDL / Q000097:blood*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology; D009195:Peroxidase / Q000097:blood*
chemical_list: D008077:Lipoproteins, LDL; C099252:oxidized low density lipoprotein; D009195:Peroxidase
keywords: atherosclerosis; end-stage renal disease; inflammation; oxidative stress