title: Mouse monoclonal antibody specific for outer membrane protein A of Escherichia coli.
abstract: Outer membrane protein A (OmpA) is a major outer membrane protein of Escherichia coli and other Enterobacteriaeae. Although the structural features of OmpA have been well studied, its roles in the pathogenesis of various bacterial infections have not been fully elucidated. Here, we report the generation of mouse monoclonal antibody (MAb) 49.4-15, which specifically recognizes OmpA of E. coli, using immunoblot and confocal microscopic examinations. MAb 49.4-15 might be a useful tool for studying the expression and function of E. coli OmpA.
authors.affiliation: Department of Microbiology and Immunology, Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Abe
authors.forename: Ikuko
authors.initials: I
authors.lastname: Haruta
authors.forename: Naoko
authors.initials: N
authors.lastname: Yanagisawa
authors.forename: Junji
authors.initials: J
authors.lastname: Yagi
mesh_terms: D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000096:biosynthesis* / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D015151:Immunoblotting; D051379:Mice; D018613:Microscopy, Confocal
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D001425:Bacterial Outer Membrane Proteins; C078167:OMPA outer membrane proteins