title: [Authentication of Lonicera japonica using bidirectional PCR amplification of specific alleles].
abstract: OBJECTIVE
  To identify SNP in flos Lonicerae, and authenticate Lonicera japonica from its adulterants and the mixture by using bidirectional PCR amplification of specific alleles (Bi-PASA).
  
  
  METHODS
  SNP of L. japonica and its adulterants was identified by using ClustulW to align trnL-trnF sequences of the Lonicera genus from GenBank database. Bi-PASA primer was designed and the PCR reaction systems including annealing temperature optimized. Optimized result was performed in 84 samples to authenticate L. japonica, its adulterants and the mixture.
  
  
  RESULTS
  When the annealing temperature was 61 degrees C, DNA from L. japonica would be amplified 468 bp whereas PCR products from all of the 9 adulterants were 324 bp. The established method also can detect 5% of intentional adulteration DNA into L. japonica.
  
  
  CONCLUSIONS
  The Bi-SPASA could authenticate L. japonica from its adulterants and the mixture.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Jiang
authors.forename: Ya-Hua
authors.initials: YH
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Chen
authors.forename: Yuan
authors.initials: Y
authors.lastname: Yuan
authors.forename: Shu-Fang
authors.initials: SF
authors.lastname: Lin
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Wu
mesh_terms: D000483:Alleles; D058893:DNA Barcoding, Taxonomic / Q000379:methods; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics*; D035264:Flowers / Q000235:genetics; D031239:Lonicera / Q000145:classification / Q000235:genetics*; D010946:Plants, Medicinal / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide; D012356:RNA, Transfer, Leu / Q000235:genetics; D012360:RNA, Transfer, Phe / Q000235:genetics; D015203:Reproducibility of Results; D013045:Species Specificity
chemical_list: D018744:DNA, Plant; D012356:RNA, Transfer, Leu; D012360:RNA, Transfer, Phe