title: Improvement of drug elution in thin mineralized collagen coatings with PLGA-PEG-PLGA micelles.
abstract: A mineralized collagen (MC) coating on metallic implants has shown great potential as orthopedic material due to high biological responses. However, their drug delivery capacity remains unsatisfactory since a serious burst release may occur and long-term release is hard to be achieved. Aiming to improve the drug-eluting capability, we incorporated drug-loaded PLGA-PEG-PLGA (PPP) micelles into the thin coating. The in vitro release profiles showed that the burst release in the initial 8 h of the modified coating decreased from 81% to 58% compared to MC coating alone; meanwhile, the release duration was prolonged from 3 days to 1 week. Additionally, the release kinetics of vancomycin hydrochloride (VH, the model drug) could be adjusted by changing the size and concentration of PPP micelles. Interestingly, less initial release of VH caused by micelle immobilization did not affect the antibacterial activity in the early stage of implantation according to the antimicrobial test. The cytocompatibility assay demonstrated that the VH-loaded PPP micelles did not have negative effect on the bioactivity of coating which greatly enhanced cell activity compared to bare Ti substrates. Thus, the MC coatings with PPP micelles could be an effective implant route for bone repair.
authors.affiliation: Department of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou, 310027, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Ling
authors.forename: Mengfei
authors.initials: M
authors.lastname: Yu
authors.forename: Wenjian
authors.initials: W
authors.lastname: Weng
authors.forename: Huiming
authors.initials: H
authors.lastname: Wang
authors.forename: Kui
authors.initials: K
authors.lastname: Cheng
authors.forename: Jun
authors.initials: J
authors.lastname: Lin
authors.forename: Piyi
authors.initials: P
authors.lastname: Du
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003094:Collagen / Q000494:pharmacology*; D016503:Drug Delivery Systems; D007700:Kinetics; D051379:Mice; D008823:Micelles; D008826:Microbial Sensitivity Tests; D008903:Minerals / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D011092:Polyethylene Glycols / Q000737:chemistry*; D011098:Polyglactin 910 / Q000737:chemistry*; D016062:Porosity; D013052:Spectrometry, X-Ray Emission; D014640:Vancomycin / Q000494:pharmacology*; D014961:X-Ray Diffraction
chemical_list: D000890:Anti-Infective Agents; D020099:Coated Materials, Biocompatible; D008823:Micelles; D008903:Minerals; C520330:polyethylene glycol-poly(lactic-co-glycolic acid)-polyethylene glycol; D011098:Polyglactin 910; D011092:Polyethylene Glycols; D014640:Vancomycin; D003094:Collagen
keywords: PLGA-PEG-PLGA micelles; drug delivery; mineralized collagen; thin coating; titanium implant