title: Human platelet-specific antigen frequencies in Indonesian population.
abstract: BACKGROUND
  Alloantibodies against human platelet antigens (HPAs) are responsible for the development of alloimmune thrombocytopenia including platelet transfusion refractoriness (PTR) and neonatal alloimmune thrombocytopenia (NAIT). Therefore, transfusion of HPA-compatible platelets is of importance for the management of these diseases.
  
  
  OBJECTIVE
  Determination of the allele frequency of the major HPA systems for Indonesian blood donors and the development of the first HPA-typed donor registry in Indonesia.
  
  
  METHODS
  DNA derived from 500 Indonesian healthy blood donors was genotyped for HPA-1 to HPA-6 and HPA-15 alleles by the use of polymerase chain reaction sequence-specific primer method.
  
  
  RESULTS
  The gene frequencies of the rare allelic variants HPA-1b, -2b, -3b, -4b, -5b, -6b and -15b were 0·023, 0·060, 0·493, 0·052, 0·032, 0·044 and 0·049, respectively. However, donors homozygous for the HPA-1b, -2b and -6b were not found in this cohort, indicating that the risks of alloimmunisation caused by incompatibility of these three HPA systems are extremely low. In contrast, alloimmunisation against HPA-3, -4, -5 and -15 systems is anticipated.
  
  
  CONCLUSIONS
  The development of an HPA-genotyped registry for donors homozygous for HPA-1b, -2b and -6b is desired for the optimum management of PTR patients and children with NAIT.
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Universitas Indonesia. asmarinah.si@gmx.de
authors.lastname: Asmarinah
authors.forename: R
authors.initials: R
authors.lastname: Dharma
authors.forename: N K
authors.initials: NK
authors.lastname: Ritchie
authors.forename: S
authors.initials: S
authors.lastname: Rahayu
authors.forename: E
authors.initials: E
authors.lastname: Putricahya
authors.forename: S
authors.initials: S
authors.lastname: Santoso
mesh_terms: D016824:Antigens, Human Platelet / Q000235:genetics* / Q000378:metabolism; D001782:Blood Donors; D001787:Blood Group Incompatibility / Q000097:blood / Q000235:genetics; D001792:Blood Platelets / Q000378:metabolism; D005260:Female; D005787:Gene Frequency / Q000235:genetics*; D006801:Humans; D007214:Indonesia; D008297:Male; D017713:Platelet Transfusion / Q000009:adverse effects / Q000379:methods; D054098:Thrombocytopenia, Neonatal Alloimmune / Q000097:blood / Q000209:etiology / Q000235:genetics
chemical_list: D016824:Antigens, Human Platelet
keywords: Indonesian; gene frequency; human platelet antigen; platelet donor registry