title: Translucent titanium coating altered the composition of focal adhesions and promoted migration of osteoblast-like MG-63 cells on glass.
abstract: "TiGlass" was designed and was known to promote initial adhesion and increase migration of rat calvarial osteoblats. In this article, migration study and a series of epifluorescence microscopic studies were conducted to find out the composition of focal adhesion on titanium surface. The translucent titanium surface was applied in random migration analysis and immunofluorescence cell staining. In the immunofluorescent double staining, phosphorylated focal adhesion kinase was tested with vinculin. Various integrin subunits were then tested with vinculin to study the composition of activated focal adhesions. Integrin subunit α5 and αV were tested against β3; integrin subunits α5, αV, β3, and αVβ3 were tested with F-actin, respectively. The MG-63 cells began migration earlier and migrated faster on "TiGlass." Immunofluorescent double staining revealed that all focal adhesion kinase in the focal adhesions were activated on both the surfaces. The osteoblast was inferred to made adhesion to titanium and glass through integrins. The focal adhesions on glass were found to be composed of integrin subunits αV and β3. However, on "TiGlass," integrin subunits α5 might have supplemented the adhesion to titanium. Results from double staining of integrin subunits α5, αV, β3, and αVβ3 with F-actin also supported integrin subunits α5 might have involved in adhesion of titanium.
authors.affiliation: Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan.
authors.forename: Yi
authors.initials: Y
authors.lastname: Ho
authors.forename: Sang-Heng
authors.initials: SH
authors.lastname: Kok
authors.forename: Juo-Song
authors.initials: JS
authors.lastname: Wang
authors.forename: Li-Deh
authors.initials: LD
authors.lastname: Lin
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects*; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D005455:Fluorescent Antibody Technique; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism; D022001:Focal Adhesions / Q000187:drug effects / Q000378:metabolism*; D005898:Glass / Q000737:chemistry*; D006801:Humans; D016023:Integrins / Q000378:metabolism; D050505:Mutant Proteins / Q000378:metabolism; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D021122:Protein Subunits / Q000378:metabolism; D051381:Rats; D013194:Staining and Labeling; D014025:Titanium / Q000494:pharmacology*; D016596:Vinculin / Q000378:metabolism
chemical_list: D000199:Actins; D020099:Coated Materials, Biocompatible; D016023:Integrins; D050505:Mutant Proteins; D021122:Protein Subunits; D016596:Vinculin; D014025:Titanium; D051416:Focal Adhesion Protein-Tyrosine Kinases
keywords: adhesion; migration; osteoblast; titanium; translucent