title: Intestinal metabolism of T-2 toxin in the pig cecum model.
abstract: T-2 toxin, a toxic member of the group A trichothecenes, is produced by various Fusarium species that can potentially affect human health. As the intestine plays an important role in the metabolism of T-2 toxin for animals and humans, the degradation and metabolism of T-2 toxin was studied using the pig cecum in vitro model system developed in the author's group. In order to study the intestinal degradation of T-2 toxin by pig microbiota, incubation was performed with the cecal chyme from four different pigs in repeat determinations. A large variation in the intestinal degradation of T-2 toxin was observed for individual pigs. T-2 toxin was degraded almost completely in one out of four pigs, in which only 3.0 ± 0.1 % of T-2 toxin was left after 24 h incubation. However, in the other three incubations with pig cecal suspension, 54.1 ± 11.7-68.9 ± 16.1 % of T-2 toxin were still detectable after 24 h incubation time. The amount of HT-2 toxin was increased along with the incubation time, and HT-2 toxin accounted for 85.2 ± 0.7 % after 24 h in the most active cecum. HT-2 toxin was the only detectable metabolite formed by the intestinal bacteria. This study suggests that the toxicity of T-2 toxin for pigs is caused by the combination of T-2 and HT-2 toxins.
authors.affiliation: MOA Key Laboratory of Food Safety Evaluation/National Reference Laboratory of Veterinary Drug Residues (HZAU), Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Wu
authors.forename: Anna
authors.initials: A
authors.lastname: Engemann
authors.forename: Benedikt
authors.initials: B
authors.lastname: Cramer
authors.forename: Tanja
authors.initials: T
authors.lastname: Welsch
authors.forename: Zonghui
authors.initials: Z
authors.lastname: Yuan
authors.forename: Hans-Ulrich
authors.initials: HU
authors.lastname: Humpf
mesh_terms: D000818:Animals; D001682:Biological Availability; D002432:Cecum / Q000378:metabolism* / Q000382:microbiology; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism; D008954:Models, Biological; D013552:Swine; D013605:T-2 Toxin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D014255:Trichothecenes / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity
chemical_list: D014255:Trichothecenes; D013605:T-2 Toxin; C012351:HT-2 toxin