title: Functional characterization of Upf1 targets in Schizosaccharomyces pombe.
abstract: Nonsense-mediated mRNA decay (NMD) is a highly conserved mechanism of mRNA degradation. NMD eliminates mRNAs containing premature termination codons (PTCs), preventing the production of truncated proteins with possible deleterious effects. However, there is mounting evidence that NMD factors, like Upf1, Upf2 and Upf3, participate in general regulation of gene expression, affecting the expression of genes lacking PTCs. We have used the fission yeast Schizosaccharomyces pombe to identify mRNAs directly regulated by NMD. Using a combination of genetic and biochemical approaches, we have defined a population of fission yeast mRNAs specifically regulated by Upf1. We show that other components of the Upf complex, Upf2 and Upf3, are required for binding of Upf1 to its RNA targets and for the proper response of fission yeast to oxidative stress. Finally, we investigated the physiological importance of this phenomenon, and demonstrate that the Upf1-dependent downregulation of some of its direct targets is necessary for normal resistance to oxidative stress.
authors.affiliation: Centro de Biología Molecular Severo Ochoa, UAM-CSIC Madrid, Spain.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Matia-González
authors.forename: Ayesha
authors.initials: A
authors.lastname: Hasan
authors.forename: Gøril H
authors.initials: GH
authors.lastname: Moe
authors.forename: Juan
authors.initials: J
authors.lastname: Mata
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Rodríguez-Gabriel
mesh_terms: D018389:Codon, Nonsense / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D015966:Gene Expression Regulation, Fungal; D059365:Nonsense Mediated mRNA Decay; D018384:Oxidative Stress; D020365:RNA Helicases / Q000235:genetics / Q000378:metabolism*; D020871:RNA Stability; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism; D012568:Schizosaccharomyces / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D018389:Codon, Nonsense; D012331:RNA, Fungal; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D029702:Schizosaccharomyces pombe Proteins; C513657:Upf1 protein, S pombe; D020365:RNA Helicases
keywords: NMD; fission yeast; mRNA binding