title: Comparative surface antimicrobial properties of synthetic biocides and novel human apolipoprotein E derived antimicrobial peptides.
abstract: Medical device infection remains a major clinical concern. Biocidal compounds have been incorporated into medical device materials ideally to inhibit bacterial colonisation whilst exhibiting relatively low cytotoxicity. We compared the antibacterial activity, anti-biofilm efficacy and cytotoxicity of a novel peptide derivative of human apolipoprotein E (apoEdpL-W) to that of commonly used biocides, before and after coating onto a range of standard polymers. Since the antimicrobial function of most biocides frequently involves associations with cellular membranes, we have also studied the detailed interactions of the test antimicrobials with phospholipid bilayers, using the quartz crystal microbalance device combined with dual-polarisation interferometry. ApoEdpL-W displayed broad-spectrum antibacterial activity and marked efficacy against nascent Staphylococcus aureus biofilms. Compounds showed better antimicrobial activity when combined with hydrogel materials than with non-porous materials. The membrane interactions of apoEdpL-W were most similar to that of PHMB, with both agents appearing to readily bind and insert into lipid bilayers, possibly forming pores. However apoEdpL-W showed lower cytotoxicity than PHMB, its efficacy was less affected by the presence of serum, and it demonstrated the highest level of biocompatibility of all the biocides, as indicated by our measurement of its antimicrobial biocompatibility index. This work shows the potential of apoEdpL-W as an effective antiseptic coating agent.
authors.affiliation: Faculty of Life Sciences, The University of Manchester, Manchester, M13 9PL, UK.
authors.forename: Sarah
authors.initials: S
authors.lastname: Forbes
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: McBain
authors.forename: Susan
authors.initials: S
authors.lastname: Felton-Smith
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Jowitt
authors.forename: Holly L
authors.initials: HL
authors.lastname: Birchenough
authors.forename: Curtis B
authors.initials: CB
authors.lastname: Dobson
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000494:pharmacology*; D001057:Apolipoproteins E / Q000737:chemistry*; D001419:Bacteria / Q000187:drug effects; D001672:Biocompatible Materials / Q000494:pharmacology; D001718:Birefringence; D016923:Cell Death / Q000187:drug effects; D004202:Disinfectants / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D019793:Fluorescein / Q000378:metabolism; D006801:Humans; D008567:Membranes, Artificial; D051379:Mice; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D008969:Molecular Sequence Data; D011108:Polymers; D058525:Quartz Crystal Microbalance Techniques
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D001057:Apolipoproteins E; D001672:Biocompatible Materials; D004202:Disinfectants; D008567:Membranes, Artificial; D011108:Polymers; D019793:Fluorescein