title: A novel fluorescent "Turn-Off/Turn-On" system for the detection of acid phosphatase activity.
abstract: Acid phosphatase (ACP) can be sensitively, conveniently and efficiently detected via a new fluorescent "Turn-Off/Turn-On" system. Inexpensive (NaPO(3))(6) is carefully introduced into this system as a quencher of the aggregation-caused quenching (ACQ) probe. In our method, the limit of detection (LOD) is quite low for detecting ACP, which is an important biomarker and indication of several diseases.
authors.affiliation: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan, Hubei 430072, P.R. China.
authors.forename: Pu
authors.initials: P
authors.lastname: Guo
authors.forename: Shengyong
authors.initials: S
authors.lastname: Yan
authors.forename: Yimin
authors.initials: Y
authors.lastname: Zhou
authors.forename: Changcheng
authors.initials: C
authors.lastname: Wang
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Xu
authors.forename: Xiaocheng
authors.initials: X
authors.lastname: Weng
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhou
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism*; D000818:Animals; D057075:Enzyme Assays / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D057230:Limit of Detection; D010710:Phosphates / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D010710:Phosphates; D000135:Acid Phosphatase; C018279:sodium phosphate