title: Inhibition of BCR/ABL protein expression by miR-203 sensitizes for imatinib mesylate.
abstract: Selective inhibition of BCR/ABL expression by RNA interference has been demonstrated as an effective strategy in CML treatment and a reversal to imatinib resistance. microRNAs (miRNAs) are small regulatory RNAs involved in post-transcriptional gene regulation. miR-203 is supposed to directly regulate ABL and BCR/ABL expression, however, the role of miR-203 in imatinib-resistant cells is not clear. Here, we report that overexpression of miR-203 in BaF3-BCR/ABL cells with T315I mutant inhibited cell growth and colony formation ability. Furthermore, miR-203 increased sensitivity to imatinib in BaF3-BCR/ABL(T315I) cells, thereby antagonizing the main mechanism of resistance to imatinib.
authors.affiliation: Key Laboratory of Laboratory Medical Diagnostics and Department of Clinical Hematology Designated by the Ministry of Education, Chongqing Medical University, Chongqing, China.
authors.forename: Yajuan
authors.initials: Y
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Yuan
authors.forename: Kun
authors.initials: K
authors.lastname: Tao
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Qing
authors.initials: Q
authors.lastname: Xiao
authors.forename: Zhenglan
authors.initials: Z
authors.lastname: Huang
authors.forename: Liang
authors.initials: L
authors.lastname: Zhong
authors.forename: Weixi
authors.initials: W
authors.lastname: Cao
authors.forename: Jianping
authors.initials: J
authors.lastname: Wen
authors.forename: Wenli
authors.initials: W
authors.lastname: Feng
mesh_terms: D000328:Adult; D000818:Animals; D001549:Benzamides / Q000494:pharmacology*; D015153:Blotting, Western; D002453:Cell Cycle / Q000187:drug effects / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D016044:Fusion Proteins, bcr-abl / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D000068877:Imatinib Mesylate; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D010879:Piperazines / Q000494:pharmacology*; D011743:Pyrimidines / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D001549:Benzamides; C546703:MIRN203 microRNA, human; D035683:MicroRNAs; D010879:Piperazines; D011743:Pyrimidines; D000068877:Imatinib Mesylate; D016044:Fusion Proteins, bcr-abl