title: Application of Fourier transform-second-harmonic generation imaging to the rat cervix.
abstract: We present the application of Fourier transform-second-harmonic generation (FT-SHG) imaging to evaluate the arrangement of collagen fibers in five nonpregnant rat cervices. Tissue slices from the mid-cervix and near the external orifice of the cervix were analyzed in both two-dimensions (2D) and three-dimensions (3D). We validate that the cervical microstructure can be quantitatively assessed in three dimensions using FT-SHG imaging and observe collagen fibers oriented both in and out-of-plane in the outermost and the innermost layers, which cannot be observed using 2D FT-SHG analysis alone. This approach has the potential to be a clinically applicable method for measuring progressive changes in collagen organization during cervical remodeling in humans.
authors.affiliation: Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
authors.forename: T Y
authors.initials: TY
authors.lastname: Lau
authors.forename: H K
authors.initials: HK
authors.lastname: Sangha
authors.forename: E K
authors.initials: EK
authors.lastname: Chien
authors.forename: B L
authors.initials: BL
authors.lastname: McFarlin
authors.forename: A J
authors.initials: AJ
authors.lastname: Wagoner Johnson
authors.forename: K C
authors.initials: KC
authors.lastname: Toussaint
mesh_terms: D000818:Animals; D002584:Cervix Uteri / Q000000981:diagnostic imaging* / Q000648:ultrastructure; D003094:Collagen / Q000648:ultrastructure; D005260:Female; D021621:Imaging, Three-Dimensional / Q000379:methods*; D011859:Radiography; D051381:Rats; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D003094:Collagen