title: Repression of antibiotic downregulator WblA by AdpA in Streptomyces coelicolor.
abstract: The upstream region of antibiotic downregulatory wblA in Streptomyces coelicolor was found to contain AdpA binding motifs. A key morphological regulator, AdpA was shown to specifically bind these motifs by electrophoretic mobility shift assay. An adpA disruption mutant exhibited increased wblA transcription, suggesting that AdpA negatively regulates wblA transcription in S. coelicolor.
authors.affiliation: Department of Biological Engineering, Inha University, Incheon, Republic of Korea.
authors.forename: Han-Na
authors.initials: HN
authors.lastname: Lee
authors.forename: Jin-Su
authors.initials: JS
authors.lastname: Kim
authors.forename: Pil
authors.initials: P
authors.lastname: Kim
authors.forename: Heung-Shick
authors.initials: HS
authors.lastname: Lee
authors.forename: Eung-Soo
authors.initials: ES
authors.lastname: Kim
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D024202:Electrophoretic Mobility Shift Assay; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic / Q000235:genetics; D017422:Sequence Analysis, DNA; D048372:Streptomyces coelicolor / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D015534:Trans-Activators; D014157:Transcription Factors; C081543:whiB protein, Streptomyces