title: Scaling up, characterization of levan and its inhibitory role in carcinogenesis initiation stage'.
abstract: Honey isolate Bacillus subtilis M was cultivated in shake flasks and in 16-l bioreactor cultures to investigate cell growth, bio-metabolites production kinetics and bioprocess scalability. The respective maximal levan and levansucrase productions of 59.5 g/l and 74.1 U/ml were achieved in bioreactor cultures under pH controlled condition (pH=7.0) after only 24 h. Crude levan (levE) was isolated, characterized and fractionated into F1, F2, and F3 with different molecular weight (21.8, 13.118, 9.53 kDa). (1)H NMR and (13)C NMR spectroscopy proved that LevE and their fractions were mainly β-(2, 6)-linked levan-type polysaccharide. The cancer chemo-preventive activity indicated that the levE and its fraction 3 were promising inhibitors of cytochrome P-450 1A activity, inducers of glutathione-S-transferase activity in Murine hepatomaHepa1c1c7cells and possessed highest radical scavenging affinity to both ROO and OH. They inhibited the induced-DNA fragmentation. None of the tested samples triggered apoptosis or necrosis in splenocytes, except F2.
authors.affiliation: Department of Chemistry of Natural and Microbial Products, National Research Centre, Dokki, Giza, Egypt. mona_esawy@hotmail.com
authors.forename: Mona A
authors.initials: MA
authors.lastname: Esawy
authors.forename: Hassan
authors.initials: H
authors.lastname: Amer
authors.forename: Amira M
authors.initials: AM
authors.lastname: Gamal-Eldeen
authors.forename: Hesham A
authors.initials: HA
authors.lastname: El Enshasy
authors.forename: Wafaa A
authors.initials: WA
authors.lastname: Helmy
authors.forename: Mona A M
authors.initials: MA
authors.lastname: Abo-Zeid
authors.forename: Roslinda
authors.initials: R
authors.lastname: Malek
authors.forename: Eman F
authors.initials: EF
authors.lastname: Ahmed
authors.forename: Ghada E A
authors.initials: GE
authors.lastname: Awad
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D000975:Antioxidants / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D001412:Bacillus subtilis / Q000378:metabolism*; D019149:Bioreactors; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D019363:Cytochrome P-450 CYP1A1 / Q000037:antagonists & inhibitors; D053938:DNA Fragmentation; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D005630:Fructans / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D005982:Glutathione Transferase / Q000378:metabolism; D006602:Hexosyltransferases / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D015162:Micronucleus Tests; D009369:Neoplasms / Q000201:enzymology*; D013154:Spleen / Q000166:cytology
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D004791:Enzyme Inhibitors; D005630:Fructans; C072599:levan; D019363:Cytochrome P-450 CYP1A1; D006602:Hexosyltransferases; C021513:levansucrase; D005982:Glutathione Transferase