title: Secreted osteoclastogenic factor of activated T cells (SOFAT), a novel osteoclast activator, in chronic periodontitis.
abstract: A novel activated human T cell-secreted cytokine, referred as secreted osteoclastogenic factor of activated T cells (SOFAT), that induce osteoclastogenesis in a RANKL-independent manner was recently described. This study evaluated the role of SOFAT in periodontal tissues and periodontitis. Gingival biopsies were harvested from systemically healthy non-periodontitis (n=15) and chronic periodontitis patients (n=15). The mRNA and protein levels of SOFAT were measured by qPCR and by enzyme-linked immunosorbent assay, respectively. Moreover, RAW 264.7 cells were cultured with SOFAT or Receptor activator of nuclear factor-kB ligand (RANKL) and stained for tartrate-resistant acid phosphatase (TRAP). Also, mice received a palatal injection between the first and second upper molar of SOFAT (100 ng/ml) or saline solution (0.9%). The upper jaw was removed, histologically processed and stained with hematoxilin and eosin to observe the presence of osteoclast-like cells. The mRNA and protein levels of SOFAT were significantly higher in the gingival tissue of the periodontitis group when compared to non-periodontitis one (p<0.05). In addition, SOFAT potently induced TRAP-positive multinucleated cell formation by RAW 264.7 cells as well as induced the formation of osteoclast-like cells in the periodontal ligament in mice. The present study demonstrated that SOFAT may play an important role in periodontitis.
authors.affiliation: Laboratory of Immunology and Molecular Biology, São Leopoldo Mandic Institute and Research Center, Campinas/SP, Brazil.
authors.forename: Christian Rado
authors.initials: CR
authors.lastname: Jarry
authors.forename: Poliana Mendes
authors.initials: PM
authors.lastname: Duarte
authors.forename: Fabiana Furtado
authors.initials: FF
authors.lastname: Freitas
authors.forename: Cristina Gomes
authors.initials: CG
authors.lastname: de Macedo
authors.forename: Juliana Trindade
authors.initials: JT
authors.lastname: Clemente-Napimoga
authors.forename: Eduardo
authors.initials: E
authors.lastname: Saba-Chujfi
authors.forename: Fabricio
authors.initials: F
authors.lastname: Passador-Santos
authors.forename: Vera Cavalcanti
authors.initials: VC
authors.lastname: de Araújo
authors.forename: Marcelo Henrique
authors.initials: MH
authors.lastname: Napimoga
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000328:Adult; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D055113:Chronic Periodontitis / Q000235:genetics / Q000378:metabolism*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D008213:Lymphocyte Activation; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D010010:Osteoclasts / Q000276:immunology*; D010012:Osteogenesis; D053245:RANK Ligand / Q000378:metabolism*; D013601:T-Lymphocytes / Q000378:metabolism; D000071681:Tartrate-Resistant Acid Phosphatase
chemical_list: D016207:Cytokines; D007527:Isoenzymes; D053245:RANK Ligand; C546006:THNSL2 protein, human; D000135:Acid Phosphatase; C000605511:Acp5 protein, mouse; D000071681:Tartrate-Resistant Acid Phosphatase