title: Antitumor and immunomodulatory activity of a polysaccharide from fungus Coprinus comatus (Mull.:Fr.) Gray.
abstract: In our study, a water-soluble polysaccharide (CCPa-1) was successfully purified from the fruiting bodies of Coprinus comatus by DEAE-cellulose and Sepharose CL-6B column chromatography. The molecular weight was evaluated to be 53.6 kDa as determined by high performance size exclusion chromatography (HPSEC). Sugar composition analysis revealed that CCPa-1 consisted primarily of galactose, glucose and arabinose in a molar ratio of 6.6:1.2:2.2. CCPa-1 could not only inhibit the growth of sarcoma 180 (S180) tumor transplanted in mice, but also increase the relative spleen/thymus indexes and body weight of tumor bearing mice. Moreover, Con A- or LPS-induced splenocyte proliferation was also enhanced after CCPa-1 administration in tumor-bearing mice. Furthermore, CCPa-1 significantly enhanced the Con A- or LPS-induced splenocyte proliferation and increased the production of TNF-α and IL-2. All the data demonstrated that CCPa-1 had a potential application as natural antitumor agent with immunomodulatory activity.
authors.affiliation: Department of Genetics and Molecular Biology, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi 710061, PR China.
authors.forename: Xiao-Gang
authors.initials: XG
authors.lastname: Jiang
authors.forename: Ming-Xue
authors.initials: MX
authors.lastname: Lian
authors.forename: Yan
authors.initials: Y
authors.lastname: Han
authors.forename: She-Min
authors.initials: SM
authors.lastname: Lv
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D003302:Coprinus / Q000737:chemistry*; D062610:Fungal Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D007155:Immunologic Factors / Q000302:isolation & purification / Q000494:pharmacology*; D007376:Interleukin-2 / Q000097:blood; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D012513:Sarcoma, Experimental / Q000188:drug therapy / Q000276:immunology / Q000473:pathology; D013154:Spleen / Q000187:drug effects / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: D000970:Antineoplastic Agents; D062610:Fungal Polysaccharides; D007155:Immunologic Factors; D007376:Interleukin-2; D014409:Tumor Necrosis Factor-alpha