title: Unusual glycosaminoglycans from a deep sea hydrothermal bacterium improve fibrillar collagen structuring and fibroblast activities in engineered connective tissues.
abstract: Biopolymers produced by marine organisms can offer useful tools for regenerative medicine. Particularly, HE800 exopolysaccharide (HE800 EPS) secreted by a deep-sea hydrothermal bacterium displays an interesting glycosaminoglycan-like feature resembling hyaluronan. Previous studies demonstrated its effectiveness to enhance in vivo bone regeneration and to support osteoblastic cell metabolism in culture. Thus, in order to assess the usefulness of this high-molecular weight polymer in tissue engineering and tissue repair, in vitro reconstructed connective tissues containing HE800 EPS were performed. We showed that this polysaccharide promotes both collagen structuring and extracellular matrix settle by dermal fibroblasts. Furthermore, from the native HE800 EPS, a low-molecular weight sulfated derivative (HE800 DROS) displaying chemical analogy with heparan-sulfate, was designed. Thus, it was demonstrated that HE800 DROS mimics some properties of heparan-sulfate, such as promotion of fibroblast proliferation and inhibition of matrix metalloproteinase (MMP) secretion. Therefore, we suggest that the HE800EPS family can be considered as an innovative biotechnological source of glycosaminoglycan-like compounds useful to design biomaterials and drugs for tissue engineering and repair.
authors.affiliation: Biochemistry Department, Dental School, Paris Descartes University, PRES Sorbonne Paris Cité, 1 rue Maurice Arnoux, Montrouge 92120, France. senni_k@yahoo.com
authors.forename: Karim
authors.initials: K
authors.lastname: Senni
authors.forename: Farida
authors.initials: F
authors.lastname: Gueniche
authors.forename: Sylvie
authors.initials: S
authors.lastname: Changotade
authors.forename: Dominique
authors.initials: D
authors.lastname: Septier
authors.forename: Corinne
authors.initials: C
authors.lastname: Sinquin
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Ratiskol
authors.forename: Didier
authors.initials: D
authors.lastname: Lutomski
authors.forename: Gaston
authors.initials: G
authors.lastname: Godeau
authors.forename: Jean
authors.initials: J
authors.lastname: Guezennec
authors.forename: Sylvia
authors.initials: S
authors.lastname: Colliec-Jouault
mesh_terms: D001672:Biocompatible Materials / Q000302:isolation & purification / Q000494:pharmacology; D001709:Biotechnology / Q000379:methods; D049109:Cell Proliferation / Q000187:drug effects; D003238:Connective Tissue / Q000378:metabolism; D024022:Fibrillar Collagens / Q000737:chemistry; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D006025:Glycosaminoglycans / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D011135:Polysaccharides, Bacterial / Q000302:isolation & purification / Q000494:pharmacology*; D023822:Tissue Engineering / Q000379:methods*; D014733:Vibrio / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D024022:Fibrillar Collagens; D006025:Glycosaminoglycans; D011135:Polysaccharides, Bacterial