title: [Polymorphism of regulatory regions of growth factor gene of vascular endothelium VEGFA in breast cancer patients with family history].
abstract: Analyzed the association of functional polymorphisms of VEGFA at positions -2578 of promoter region and at position +936 3' of untranslated region in a group of healthy women and breast cancer patients with the aim to detect informative markers associated with the risk of disease. The study included 395 DNA samples from women with breast cancer and 298 healthy women. Genotyping of polymorphisms of C-2578A and C +936 T VEGFA was performed by restrictase analysis of amplification products (RFLP-analysis). There was revealed the increase of the frequency-2578AA genotype in patients with breast cancer (OR 1.55, 95% CI 1,55-2,29), moreover, the frequency of this genotype increased in the group of patients with a common family history and with breast cancer in close relatives of relatively healthy women (OR = 2,03 95% CI 1,26-3,27 and OR = 2,22 95% CI 1,13-4,33, respectively). Genotype frequencies of -2578AA/+ 936 SS and -2578SS/+ 936 CT were significantly higher in the group of patients with a factor of family history as compared to the group of patients without it (OR = 1,84 95% CI 1,01-3,34 and OR = 2,91 95% CI 1,09-8,05 respectively).
authors.forename: A V
authors.initials: AV
authors.lastname: Shevchenko
authors.forename: V I
authors.initials: VI
authors.lastname: Konenkov
authors.forename: E Iu
authors.initials: EIu
authors.lastname: Garbukov
authors.forename: M N
authors.initials: MN
authors.lastname: Stakheeva
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001943:Breast Neoplasms / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D016017:Odds Ratio; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics; D012307:Risk Factors; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A