title: Free radical scavenging and total antioxidant capacity of root extracts of Anchomanes difformis Engl. (Araceae).
abstract: Antioxidants activities from plants sources have attracted a wide range of interest across the world in recent times. This is due to growing concern for safe and alternative sources of antioxidants. The free radical scavenging activity using 1,1-diphenyl-2-picrylhydrazyl radical (DPPH), reducing power assay, total antioxidant capacity of the phosphomolybdenum method and the total phenolics content using the Folin-Ciocalteu reagent were carried out on the acetone, n-butanol and methanol root extracts of Anchomanes difformis. The results of the total phenolics content expressed in mg/100 g of gallic acid equivalent (GAE) showed that the n-butanol extract has significantly (p < 0.05) higher phenolics content (381 +/- 1.13) than the methanol and acetone extracts. All the extracts displayed strong concentration dependent radical scavenging activity. It was also observed that the n-butanol extract showed higher activity of 70.87% and 78.59% at low concentrations of 31.25 microg/mL and 62.5 microg/mL, respectively, than methanol and acetone extracts. The results also showed that the n-butanol extract has strongest reducing ability which is comparable to that of gallic acid at all the concentrations tested. Phytochemical screening on the extracts revealed the presence of flavonoids, saponins, and tannins. The results suggest that n-butanol extract of the plant is very rich in antioxidant compounds worthy of further investigations.
authors.affiliation: Department of Chemistry, Ahmadu Bello University, Zaria, Nigeria. aliyubabando@gmail.com
authors.forename: Abubakar B
authors.initials: AB
authors.lastname: Aliyu
authors.forename: Mohammed A
authors.initials: MA
authors.lastname: Ibrahim
authors.forename: Aliyu M
authors.initials: AM
authors.lastname: Musa
authors.forename: Aisha O
authors.initials: AO
authors.lastname: Musa
authors.forename: Joyce J
authors.initials: JJ
authors.lastname: Kiplimo
authors.forename: Adebayo O
authors.initials: AO
authors.lastname: Oyewale
mesh_terms: D020001:1-Butanol / Q000737:chemistry; D000096:Acetone / Q000737:chemistry; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D029064:Araceae / Q000737:chemistry*; D001713:Biphenyl Compounds / Q000737:chemistry; D016166:Free Radical Scavengers / Q000737:chemistry; D005707:Gallic Acid / Q000737:chemistry; D000432:Methanol / Q000737:chemistry; D008982:Molybdenum / Q000737:chemistry; D010636:Phenols / Q000032:analysis; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D018517:Plant Roots; D010946:Plants, Medicinal; D012997:Solvents / Q000737:chemistry; D017973:Tungsten Compounds / Q000737:chemistry
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; C029556:Folin's phenol reagent; D016166:Free Radical Scavengers; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D012997:Solvents; D017973:Tungsten Compounds; D000096:Acetone; D005707:Gallic Acid; D008982:Molybdenum; D020001:1-Butanol; C004931:1,1-diphenyl-2-picrylhydrazyl; D000432:Methanol