title: A generic micropatterning platform to direct human mesenchymal stem cells from different origins towards myogenic differentiation.
abstract: Human mesenchymal stem cells (MSCs) derived from various origins show varied differentiation capability. Recent work shows that cell shape manipulation via micropatterning can modulate the differentiation of bone-marrow-derived MSCs. Herein, the effect of micropatterning on the myogenesis of MSCs isolated from three different sources (bone marrow, fetal tissue, and adipose) is reported. All the well-aligned cells, regardless of source, predominantly commit to myogenic lineage, as shown by the significant upregulation of myogenic gene markers and positive myosin heavy chain staining. It is demonstrated that our novel micropattern can be used as a generic platform for inducing myogenesis of MSCs from different sources and may also have the potential to be extended to induce other lineage commitment.
authors.affiliation: Division of Systems and Engineering Management, School of Mechanical and Aerospace Engineering, Nanyang Technological Univeristy, 50 Nanyang Avenue, 639798 Singapore, Singapore.
authors.forename: Ting
authors.initials: T
authors.lastname: Yu
authors.forename: Chee Kai
authors.initials: CK
authors.lastname: Chua
authors.forename: Chor Yong
authors.initials: CY
authors.lastname: Tay
authors.forename: Feng
authors.initials: F
authors.lastname: Wen
authors.forename: Haiyang
authors.initials: H
authors.lastname: Yu
authors.forename: Jerry K Y
authors.initials: JK
authors.lastname: Chan
authors.forename: Mark S K
authors.initials: MS
authors.lastname: Chong
authors.forename: David Tai
authors.initials: DT
authors.lastname: Leong
authors.forename: Lay Poh
authors.initials: LP
authors.lastname: Tan
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D001854:Bone Marrow Cells / Q000166:cytology; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects; D019070:Cell Lineage / Q000187:drug effects / Q000235:genetics; D004129:Dimethylpolysiloxanes / Q000494:pharmacology; D005333:Fetus / Q000166:cytology; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007150:Immunohistochemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D024510:Muscle Development / Q000187:drug effects / Q000235:genetics; D055495:Neurogenesis / Q000187:drug effects / Q000235:genetics; D010012:Osteogenesis / Q000187:drug effects / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D004129:Dimethylpolysiloxanes; D012333:RNA, Messenger