title: [Development and evaluation of a quantitative double antibodies sandwich ELISA assay for rIFN-alpha1b].
abstract: OBJECTIVE
  To develop a double antibody sandwich ELISA assay for quantitative determination of recombinant human interferon alpha1b.
  
  
  METHODS
  Mouse monoclonal antibodies with different binding site on rIFN-alpha1b were screened to select optimized candidates as coating and HRP-labeled index antibodies respectively. And a double antibodies sandwich ELISA was assembled; the reliable lower detection limit, specificity, accuracy and reproducibility were evaluated and validated.
  
  
  RESULTS
  The quantitative sandwich ELISA had a reliable lower detection limit of 10 ng/ml, with a liner detection range 10-100 ng/ml (R2 = 0.992), variation coefficient inter-plates is less than 10%.
  
  
  CONCLUSIONS
  The developed sandwich ELISA was a sensitive and specific, accuracy and reproducibility method for quantitative determination of recombinant human interferon alpha1b in final product.
authors.affiliation: Daxing Industrial Development Zone, Beijing, China. mywu5@sohu.com
authors.forename: Mei-Ying
authors.initials: MY
authors.lastname: Wu
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Ai
authors.forename: Yan
authors.initials: Y
authors.lastname: Cao
authors.forename: Shuang
authors.initials: S
authors.lastname: Wu
authors.forename: Xiao-Xia
authors.initials: XX
authors.lastname: Niu
authors.forename: Yong-Qing
authors.initials: YQ
authors.lastname: Cheng
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D016898:Interferon-alpha / Q000097:blood*; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000911:Antibodies, Monoclonal; D016898:Interferon-alpha