title: Cuf2 boosts the transcription of APC/C activator Fzr1 to terminate the meiotic division cycle.
abstract: The number of nuclear divisions in meiosis is strictly limited to two. Although the precise mechanism remains unknown, this seems to be achieved by adjusting the anaphase-promoting complex/cyclosome (APC/C) activity to degrade cyclin. Here, we describe a fission yeast cuf2 mutant that enters into a third nuclear division cycle, represented by ectopic spindle assembly and abnormal chromosome segregation. Cuf2 is a meiotic transcription factor, and its critical target is fzr1(+)/mfr1(+), which encodes a meiotic APC/C activator. fzr1Δ also enters a third nuclear division. Thus, Cuf2 ensures termination of the M-phase cycle by boosting Fzr1 expression to generate functional gametes.
authors.affiliation: Laboratory of Gene Function, Kazusa DNA Research Institute, 2-6-7 Kazusa-kamatari, Kisarazu, Chiba 292-0818, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Aoi
authors.forename: Kunio
authors.initials: K
authors.lastname: Arai
authors.forename: Masaya
authors.initials: M
authors.lastname: Miyamoto
authors.forename: Yuji
authors.initials: Y
authors.lastname: Katsuta
authors.forename: Akira
authors.initials: A
authors.lastname: Yamashita
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Sato
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamamoto
mesh_terms: D064200:Cdh1 Proteins; D018797:Cell Cycle Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D019926:Cyclin B / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D055786:Gene Knockout Techniques; D008540:Meiosis; D012568:Schizosaccharomyces / Q000166:cytology / Q000502:physiology*; D029702:Schizosaccharomyces pombe Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D059010:Single-Cell Analysis; D013172:Spores, Fungal / Q000166:cytology / Q000502:physiology; D014158:Transcription, Genetic
chemical_list: C498823:Cdc13 protein, S pombe; D064200:Cdh1 Proteins; D018797:Cell Cycle Proteins; C574673:Cuf2 protein, S pombe; D019926:Cyclin B; C432695:Mes1 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins; C577587:mfr1 protein, S pombe