title: Preparation of functional magnetic nanoparticles mediated with PEG-4000 and application in Pseudomonas aeruginosa rapid detection.
abstract: A rapid detection method of Pseudomonas aeruginosa based on magnetic separation and chemiluminescence was developed in this paper. Magnetic nanoparticles (MNPs) were prepared by solvothermal method with PEG-4000 as a surfactant, and then were modified. The prepared MNPs present a uniform morphology and good dispersion. The sizes of MNPs can be controlled by adjusting the dosage of FeCl3 x 6H2O. The obtained particles were characterized with Scanning electron microscope (SEM), Transmission electronic microscopy (TEM) and Fourier transform infrared (FTIR). The biotin-dUTP-labeled DNA fragments of gyrB gene were amplified by polymerase chain reaction (PCR), and Pseudomonas aeruginosa was successfully detected with detection limit as low as 7.5 fM of gyrB fragments.
authors.affiliation: State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Tang
authors.forename: Zhiyang
authors.initials: Z
authors.lastname: Li
authors.forename: Nongyue
authors.initials: N
authors.lastname: He
authors.forename: Liming
authors.initials: L
authors.lastname: Zhang
authors.forename: Chao
authors.initials: C
authors.lastname: Ma
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Li
authors.forename: Chuanyan
authors.initials: C
authors.lastname: Li
authors.forename: Zhifei
authors.initials: Z
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Deng
authors.forename: Lei
authors.initials: L
authors.lastname: He
mesh_terms: D004587:Electrophoresis, Agar Gel; D008163:Luminescent Measurements; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011092:Polyethylene Glycols / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification* / Q000648:ultrastructure; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D058185:Magnetite Nanoparticles; D011092:Polyethylene Glycols