title: A carbon sandwich environmental cell for wet specimens.
abstract: A carbon sandwich environmental cell for wet specimens was developed for environmental transmission electron microscopy (E-TEM). A plastic film with many holes was used to form carbon capsules. The carbon sandwich environmental cells were composed of the spacer and two carbon films and enclosing the samples and experimental solution. The thickness of the water layer can be controlled by changing the conditions used to prepare the plastic spacers. The quality of the images was improved over our previous E-TEM, which could circulate gas around samples. A resolution of 2 nm was obtained by using loosely condensed DNAs.
authors.affiliation: Department of Applied Physics, Tokyo University of Agriculture and Technology, Koganei, Tokyo 184-8588, Japan.
authors.forename: Yuhri
authors.initials: Y
authors.lastname: Inayoshi
authors.forename: Hiroki
authors.initials: H
authors.lastname: Minoda
mesh_terms: D002244:Carbon / Q000737:chemistry; D046529:Microscopy, Electron, Transmission / Q000295:instrumentation / Q000379:methods*; D011487:Protein Conformation; D013048:Specimen Handling / Q000379:methods*
chemical_list: D002244:Carbon
keywords: biological specimen; environmental TEM; wet specimen