title: Different photochemical events of a genetically encoded phenyl azide define and modulate GFP fluorescence.
abstract: Expanding the genetic code opens new avenues to modulate protein function in real time. By genetically incorporating photoreactive phenyl azide, the fluorescent properties of green fluorescent protein (GFP) can be modulated by light. Depending on the residue in GFP programmed to incorporate the phenyl azide, different effects on function and photochemical pathways are observed.
authors.affiliation: School of Biosciences, Cardiff University, Cardiff, UK.
authors.forename: Samuel C
authors.initials: SC
authors.lastname: Reddington
authors.forename: Pierre J
authors.initials: PJ
authors.lastname: Rizkallah
authors.forename: Peter D
authors.initials: PD
authors.lastname: Watson
authors.forename: Rachel
authors.initials: R
authors.lastname: Pearson
authors.forename: Eric M
authors.initials: EM
authors.lastname: Tippmann
authors.forename: D Dafydd
authors.initials: DD
authors.lastname: Jones
mesh_terms: D001386:Azides / Q000737:chemistry*; D005453:Fluorescence; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000235:genetics*; D010777:Photochemistry; D015202:Protein Engineering
chemical_list: D001386:Azides; D049452:Green Fluorescent Proteins