title: Pectic polysaccharides isolated from Malian medicinal plants protect against Streptococcus pneumoniae in a mouse pneumococcal infection model.
abstract: The aim of this study was to investigate whether the pectic polysaccharides BP-II, Oc50A1.I.A and CC1P1 isolated from the Malian medicinal plants Biophytum petersianum, Opilia celtidifolia and Cola cordifolia, respectively, were able to protect against Streptococcus pneumoniae infection in mice. The pectin preparations were administered intraperitoneally 3 h before challenge with S. pneumoniae serotype 6B. Blood samples were obtained from all animals before and at 3 h, 24 h and 72 h after challenge with the pneumococci. The number of bacteria in blood was recorded and the blood concentration of a range of cytokines measured. The pretreatment with BP-II, Oc50A1.I.A and CC1P1 demonstrated a protective activity against S. pneumoniae serotype 6B infection, albeit at different range of concentrations. The pectins showed no direct antibacterial effects towards S. pneumonia; however, they induced the production of a range of cytokines and chemokines. We have previously shown that BP-II, Oc50A1.I.A and CC1P1 exhibit complement fixation activity and also that BP-II and Oc50A1.I.A stimulate macrophages to produce NO. The observed clinical effect might therefore be linked to the ability of the pectic polysaccharides to stimulate the innate immune system.
authors.affiliation: Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, N-0316 Oslo, Norway. k.t.inngjerdingen@farmasi.uio.no
authors.forename: K T
authors.initials: KT
authors.lastname: Inngjerdingen
authors.forename: B K
authors.initials: BK
authors.lastname: Langerud
authors.forename: H
authors.initials: H
authors.lastname: Rasmussen
authors.forename: T K
authors.initials: TK
authors.lastname: Olsen
authors.forename: I
authors.initials: I
authors.lastname: Austarheim
authors.forename: T E
authors.initials: TE
authors.lastname: Grønhaug
authors.forename: I S
authors.initials: IS
authors.lastname: Aaberge
authors.forename: D
authors.initials: D
authors.lastname: Diallo
authors.forename: B S
authors.initials: BS
authors.lastname: Paulsen
authors.forename: T E
authors.initials: TE
authors.lastname: Michaelsen
mesh_terms: D000818:Animals; D016470:Bacteremia / Q000276:immunology / Q000517:prevention & control; D018925:Chemokines / Q000097:blood / Q000276:immunology / Q000378:metabolism; D016207:Cytokines / Q000097:blood / Q000276:immunology / Q000378:metabolism; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D008070:Lipopolysaccharides / Q000276:immunology / Q000494:pharmacology; D051379:Mice; D008826:Microbial Sensitivity Tests; D010368:Pectins / Q000276:immunology* / Q000302:isolation & purification / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry*; D011008:Pneumococcal Infections / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control; D012703:Serotyping; D013296:Streptococcus pneumoniae / Q000145:classification / Q000187:drug effects / Q000276:immunology*; D013997:Time Factors
chemical_list: D018925:Chemokines; D016207:Cytokines; D008070:Lipopolysaccharides; D010368:Pectins