title: Physicochemical properties and antioxidant activities of acidic polysaccharides from wampee seeds.
abstract: Two novel polysaccharide fractions, named WP1 and WP3, were extracted and purified from wampee [Clausena lansium (Lour.) Skeels] seeds. Their physicochemical properties, in vitro and in vivo antioxidant activities were investigated. Results indicated that both WP1 and WP3 were acidic heteropolysaccharides with uronic acid content of 45.35% and 56.79%, respectively. The molar ratio of rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, arabinose of WP1 was 5.01:1.50:39.53:1:10.76:23.50 while WP3 was 5.76:1.73:27.27:1:4.72:8.77. Furthermore, WP3 not only exhibited stronger in vitro antioxidant activities than WP1, but also more significantly increased the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) level in serum of SD rats induced by high-fat diet.
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wu
authors.forename: Tian
authors.initials: T
authors.lastname: Min
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: Furao
authors.initials: F
authors.lastname: Lai
authors.forename: Yuqian
authors.initials: Y
authors.lastname: Tang
authors.forename: Xihong
authors.initials: X
authors.lastname: Yang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D001089:Arabinose / Q000737:chemistry; D054700:Clausena / Q000737:chemistry*; D059305:Diet, High-Fat; D005690:Galactose / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D005979:Glutathione Peroxidase / Q000097:blood; D006603:Hexuronic Acids / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008297:Male; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012210:Rhamnose / Q000737:chemistry; D012639:Seeds / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000097:blood
chemical_list: D000975:Antioxidants; D006603:Hexuronic Acids; D011134:Polysaccharides; C007819:galacturonic acid; D020723:Glucuronic Acid; D001089:Arabinose; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005947:Glucose; D012210:Rhamnose; D005690:Galactose