title: Creation of endoglucanase-secreting Streptomyces lividans for enzyme production using cellulose as the carbon source.
abstract: We screened for high-activity endoglucanase (EG) as a first step toward the creation of cellulose-assimilating Streptomyces lividans transformants. EGs derived from Thermobifida fusca YX, Tfu0901, and S. lividans, cellulase B (CelB), were successfully expressed. Genes encoding Tfu0901 or CelB were introduced into S. lividans using the integrative vector pTYM18 and the high-copy-number vector pUC702, and EG activity was detected in the supernatant of each transformant. To achieve coexpression of EG and transglutaminase, the transglutaminase gene was introduced into EG-secreting S. lividans using pUC702. S. lividans coexpressing EG and transglutaminase effectively assimilated phosphoric acid swollen cellulose. The yield of Streptomyces cinnamoneus transglutaminase in the culture supernatant was 7.2 mg/L, which was 18 times higher than that of the control strain. To demonstrate the versatility of our system, we also created an EG-producing S. lividans transformant capable of coexpressing endoxylanase. The EG-secreting S. lividans transformants constructed here can be used to produce other useful compounds through cellulose fermentation.
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.
authors.forename: Shuhei
authors.initials: S
authors.lastname: Noda
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Kawai
authors.forename: Takaya
authors.initials: T
authors.lastname: Miyazaki
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Tanaka
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
mesh_terms: D001709:Biotechnology / Q000379:methods; D002244:Carbon / Q000378:metabolism*; D002480:Cellulase / Q000235:genetics / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D015870:Gene Expression; D060847:Metabolic Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D048170:Streptomyces lividans / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D011503:Transglutaminases / Q000235:genetics / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D002244:Carbon; D002482:Cellulose; D011503:Transglutaminases; D002480:Cellulase