title: Transcriptome sequencing, annotation and expression analysis of Nannochloropsis sp. at different growth phases.
abstract: Nannochloropsis sp. is an oil-rich microalga. The lipid content of cells at stationary growth phase was much higher than that of cells at logarithm growth phase, but very little information focusing on the abundances of gene transcripts concerning lipid accumulation is available. In present study, the second generation Illumina sequencing was used to investigate the genes and pathways of Nannochloropsis sp. in order to identify those involved in lipid metabolism. In total, 29,203 unigenes were obtained from logarithm and stationary growth phases. The transcripts of some unigenes were differentially abundant at two growth phases. Among the unigenes obtained, about 195 unigenes were involved in lipid metabolism and 315 unigenes were putatively transcription factors. These genes are a very useful resource for the future studies of Nannochloropsis sp.
authors.affiliation: Research Center for Marine Ecology, The First Institute of Oceanography, State Oceanic Administration of China, No.6 Xianxialing Road, Hightech Park, Qingdao 266061, PR China.
authors.forename: Minggang
authors.initials: M
authors.lastname: Zheng
authors.forename: Jinhu
authors.initials: J
authors.lastname: Tian
authors.forename: Guanpin
authors.initials: G
authors.lastname: Yang
authors.forename: Li
authors.initials: L
authors.lastname: Zheng
authors.forename: Guogang
authors.initials: G
authors.lastname: Chen
authors.forename: Jiluan
authors.initials: J
authors.lastname: Chen
authors.forename: Bo
authors.initials: B
authors.lastname: Wang
mesh_terms: D019295:Computational Biology / Q000379:methods; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D053858:Metabolic Networks and Pathways; D058086:Microalgae / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D058977:Molecular Sequence Annotation; D020411:Oligonucleotide Array Sequence Analysis; D058009:Stramenopiles / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome
chemical_list: D014157:Transcription Factors