title: Therapeutic effect of Rhizoma Dioscoreae Nipponicae on gouty arthritis based on the SDF-1/CXCR 4 and p38 MAPK pathway: an in vivo and in vitro study.
abstract: Rhizoma Dioscoreae Nipponicae (RDN) is a widely used traditional Chinese herb, which is used to treat arthroncus, arthrodynia and arthritis. As is known to us, inflammatory mechanisms have played an important role in the occurrence, course and prognosis of gouty arthritis (GA). The aim of this study was to determine the characteristic expressed proteins of synovium in GA rat and synovial cell. The rat model of GA was induced by monosodium urate (MSU) crystal. Tissue samples were assayed by immunohistochemical method. The effects of RDN on Stromal cell-derived factor 1 (SDF-1), CXCR 4 and p38 mitogen-activated protein kinase (MAPK) were investigated in MSU crystal-induced rat. The levels of SDF-1 and mitogen-activated kinase kinase (MKK) 3/6 were measured by Western Blot in interleukin-1β (IL-1β) incubated fibroblast-like synoviocytes (FLS). A significant increase in the levels of SDF-1, CXCR 4 and p38 MAPK were observed in MSU crystal-induced rat. The increased SDF-1 and MKK 3/6 levels were observed in IL-1β incubated FLS. With the treatment of RDN, the above changes were reverted back to near normal levels. RDN might have some therapeutic effects on GA through SDF-1/CXCR 4 and p38 MAPK pathway, and dioscin may be the active compound in RDN to exert therapeutic effect on GA.
authors.affiliation: Chinese Medicine Toxicological Laboratory, Institute of Traditional Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin, 150040, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Lu
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Dong-hua
authors.initials: DH
authors.lastname: Yu
authors.forename: Xu-zhao
authors.initials: XZ
authors.lastname: Li
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhou
authors.forename: Shu-min
authors.initials: SM
authors.lastname: Liu
mesh_terms: D000818:Animals; D015210:Arthritis, Gouty / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D054377:Chemokine CXCL12 / Q000378:metabolism*; D027742:Dioscorea / Q000737:chemistry*; D004144:Diosgenin / Q000031:analogs & derivatives / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D053583:Interleukin-1beta; D048371:MAP Kinase Kinase 3 / Q000378:metabolism; D048669:MAP Kinase Kinase 6 / Q000378:metabolism; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D019718:Receptors, CXCR4 / Q000378:metabolism*; D027343:Rhizome / Q000737:chemistry; D015398:Signal Transduction / Q000187:drug effects; D013583:Synovial Membrane / Q000166:cytology / Q000187:drug effects; D014527:Uric Acid; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: C575935:CXCL12 protein, rat; D054377:Chemokine CXCL12; C486264:Cxcr4 protein, rat; D004365:Drugs, Chinese Herbal; D053583:Interleukin-1beta; D019718:Receptors, CXCR4; D014527:Uric Acid; C019357:dioscin; D048051:p38 Mitogen-Activated Protein Kinases; D048371:MAP Kinase Kinase 3; D048669:MAP Kinase Kinase 6; D004144:Diosgenin
keywords: CXCR 4; Dioscorea nipponica Makino (Dioscoreaceae); MKK 3/6; SDF-1; gouty arthritis; p38 MAPK