title: Characterization, purification of Poncirin from edible citrus Ougan (Citrus reticulate cv. Suavissima) and its growth inhibitory effect on human gastric cancer cells SGC-7901.
abstract: Poncirin is a bitter flavanone glycoside with various biological activities. Poncirin was isolated from four different tissues (flavedo, albedo, segment membrane, and juice sac) of Ougan fruit (Citrus reticulate cv. Suavissima). The highest content of poncirin was found in the albedo of Ougan fruit (1.37 mg/g DW). High speed counter-current chromatography (HSCCC) combined with D101 resin chromatography was utilized for the separation and purification of poncirin from the albedo of Ougan fruit. After this two-step purification, poncirin purity increased from 0.14% to 96.56%. The chemical structure of the purified poncirin was identified by both HPLC-PDA and LC-MS. Poncirin showed a significant in vitro inhibitory effect on the growth of the human gastric cancer cells, SGC-7901, in a dose-dependent manner. Thus, poncirin from Ougan fruit, may be beneficial for gastric cancer prevention. The purification method demonstrated here will be useful for further studies on the pharmacological mechanism of poncirin activity, as well as for guiding the consumption of Ougan fruit.
authors.affiliation: Laboratory of Fruit Quality Biology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China. adesun2006@zju.edu.cn.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhu
authors.forename: Fenglei
authors.initials: F
authors.lastname: Luo
authors.forename: Yixiong
authors.initials: Y
authors.lastname: Zheng
authors.forename: Jiukai
authors.initials: J
authors.lastname: Zhang
authors.forename: Jianzhen
authors.initials: J
authors.lastname: Huang
authors.forename: Chongde
authors.initials: C
authors.lastname: Sun
authors.forename: Xian
authors.initials: X
authors.lastname: Li
authors.forename: Kunsong
authors.initials: K
authors.lastname: Chen
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D002957:Citrus / Q000737:chemistry*; D003377:Countercurrent Distribution; D004354:Drug Screening Assays, Antitumor; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D006801:Humans; D012997:Solvents; D013056:Spectrophotometry, Ultraviolet; D013274:Stomach Neoplasms / Q000473:pathology*
chemical_list: D005419:Flavonoids; D012997:Solvents; C119641:poncirin