title: A bioluminescent assay system for whole-cell determination of hormones.
abstract: A bioluminescent-assay system was fabricated for an efficient determination of bioactive small molecules in physiological samples. The following three components were newly created for this assay system: (i) a single-chain probe exerting a 7.2-times stronger optical intensity than conventional ones, (ii) a high throughput assay device uniquely designed for the assay system with ca. one-fourth smaller standard deviation (S.D.) to samples than without the device, (iii) a buffer cocktail optimized for the assay system. The advantages of the assay system were evaluated by determining (i) the stress hormone levels in human saliva and (ii) multicolor imaging of genomic and nongenomic effects of woman sex hormones. This study guides on how to fabricate an efficient assay system for bioactive small molecules with convenience and high precision.
authors.affiliation: National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305–8569, Japan. kimu-sb@aist.go.jp
authors.forename: Sung Bae
authors.initials: SB
authors.lastname: Kim
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Suzuki
authors.forename: Akira
authors.initials: A
authors.lastname: Kimura
mesh_terms: D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry; D012739:Gonadal Steroid Hormones / Q000032:analysis; D006728:Hormones / Q000032:analysis*; D006801:Humans; D006854:Hydrocortisone / Q000032:analysis; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D012463:Saliva / Q000378:metabolism
chemical_list: D005456:Fluorescent Dyes; D012739:Gonadal Steroid Hormones; D006728:Hormones; D006854:Hydrocortisone