title: Evaluation of (131/123)I-5-iodo-2'-deoxycytidine as a novel proliferation probe in a tumor mouse model.
abstract: This study evaluated a radioiodinated deoxycytidine analog, (131)I-5-iodo-2'-deoxycytidine ([(131)I]ICdR), as a novel proliferation probe and compared it with (131)I-5-iodo-2'-deoxyuridine ([(131)I]IUdR) in a NG4TL4 sarcoma-bearing mouse model. As an imaging agent, the biological characteristics of [(123)I]IUdR is not satisfactory due to its metabolic instability and short biological half-life in vivo. With [(123)I]ICdR/SPECT it was possible to clearly delineate the tumor lesion at 1h post-injection (tumor-to-muscle ratio 7.74) in tumor-bearing mice. The results of biodistribution were consistent with those observed in scintigraphic imaging. This study demonstrated that [(131)I]ICdR is a more promising SPECT probe than [(131)I]IUdR for imaging proliferation.
authors.affiliation: Department of Biomedical Imaging and Radiological Sciences, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Wen-Yi
authors.initials: WY
authors.lastname: Chang
authors.forename: Wei-Ti
authors.initials: WT
authors.lastname: Kuo
authors.forename: Chun-Yi
authors.initials: CY
authors.lastname: Wu
authors.forename: Chih-Yuan
authors.initials: CY
authors.lastname: Lin
authors.forename: Pei-Chia
authors.initials: PC
authors.lastname: Chan
authors.forename: Chih-Chieh
authors.initials: CC
authors.lastname: Shen
authors.forename: Ren-Shan
authors.initials: RS
authors.lastname: Liu
authors.forename: Hsin-Ell
authors.initials: HE
authors.lastname: Wang
authors.forename: Chuan-Lin
authors.initials: CL
authors.lastname: Chen
mesh_terms: D000818:Animals; D001972:Bromodeoxycytidine / Q000031:analogs & derivatives; D045744:Cell Line, Tumor; D003841:Deoxycytidine / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D003857:Deoxyuridine / Q000493:pharmacokinetics*; D005260:Female; D007457:Iodine Radioisotopes / Q000493:pharmacokinetics; D007553:Isotope Labeling / Q000379:methods; D008657:Metabolic Clearance Rate; D051379:Mice; D015336:Molecular Probe Techniques; D009928:Organ Specificity; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000138:chemical synthesis / Q000493:pharmacokinetics; D012509:Sarcoma / Q000000981:diagnostic imaging* / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D007457:Iodine Radioisotopes; D019275:Radiopharmaceuticals; D003841:Deoxycytidine; D001972:Bromodeoxycytidine; C011288:ibacitabine; D003857:Deoxyuridine