title: [The expression and function of anti-apoptotic Bfl-1 in prostate cancer].
abstract: OBJECTIVE
  To determine the expression of Bcl2 related protein A1(Bfl-1) mRNA in prostate cancer cell lines and tissues, and to explore the functions of Bfl-1 in prostate adenocarcinoma.
  
  
  METHODS
  RT-PCR, real-time quantitative PCR (Q-PCR)and in situ hybridization (ISH) were used to detect the expression of Bfl-1 mRNA in prostate cancer cell lines, tissues and benign prostate hyperplasia (BPH) tissue samples. The relationship between Bfl-1 expression and clinicopathological parameters was analyzed. Antisense oligonucleotides (ASONs) were used to interfere the expression of Bfl-1 and its effects on prostate cancer cells. MTT was used to detect the survival, morphologic changes of prostate cancer cells was observed by inverted microscope.
  
  
  RESULTS
  Bfl-1 mRNA, detected by RT-PCR, Q-PCR and ISH, was overexpressed in the androgen-independent prostate cancer cell lines PC-3 and DU145, but not detectable in the androgen-dependent prostate cancer cell line LNCaP and BPH tissue samples (P < 0.05). Significantly higher Bfl-1 mRNA levels were observed in higher stage and metastatic prostate cancer cases than those without metastasis or of low stage. ASONs targeting Bfl-1 significantly inhibited androgen-independent prostate cancer cell growth (P < 0.05), cell was rounding off or fragmentation.
  
  
  CONCLUSIONS
  Bfl-1 is involved in maintaining the hormone-independent prostate cancer cell growth. Bfl-1 may become a new therapeutic target in advanced prostate cancer.
authors.affiliation: Laboratory of Pathology, Department of Pathology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Xing-Lan
authors.initials: XL
authors.lastname: Li
authors.forename: Xue-Qin
authors.initials: XQ
authors.lastname: Chen
authors.forename: Ling
authors.initials: L
authors.lastname: Nie
authors.forename: Miao
authors.initials: M
authors.lastname: Xu
authors.forename: Qiu-Yao
authors.initials: QY
authors.lastname: Li
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Shang
authors.forename: Ni
authors.initials: N
authors.lastname: Chen
authors.forename: Rui
authors.initials: R
authors.lastname: Huang
authors.forename: Hao
authors.initials: H
authors.lastname: Zeng
authors.forename: Qiao
authors.initials: Q
authors.lastname: Zhou
mesh_terms: D017209:Apoptosis; D002460:Cell Line; D002471:Cell Transformation, Neoplastic; D006801:Humans; D017403:In Situ Hybridization; D008297:Male; D015778:Minor Histocompatibility Antigens; D016376:Oligonucleotides, Antisense; D011470:Prostatic Hyperplasia / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D012333:RNA, Messenger
chemical_list: C096816:BCL2-related protein A1; D015778:Minor Histocompatibility Antigens; D016376:Oligonucleotides, Antisense; D019253:Proto-Oncogene Proteins c-bcl-2; D012333:RNA, Messenger