title: The inhibitory effect of small interference RNA protein kinase C-alpha on the experimental proliferative vitreoretinopathy induced by dispase in mice.
abstract: OBJECTIVE
  To evaluate the effects of small interference RNA protein kinase C-alpha (siRNA-PKCα) on experimental proliferative vitreoretinopathy (PVR) induced by dispase in mice.
  
  
  METHODS
  C57BL/6 mice PVR models (4-6 weeks old) were induced by intravitreal injection of dispase and then equally divided into six groups. After 1 week, the five treatment groups received 2 μL, intravitreal injections of siRNA-PKCα at a concentration of 250 nM, 500 nM, 750 nM, 1000 nM, and 1500 nM, respectively, while the negative control group received 2 μL of 500 nM no-silencing siRNA. SiRNA-PKCα was transfected by a square wave electroporator. Postoperative ophthalmic observations of lens clarity and the fundus of the eyes were performed periodically. The eyeballs of the mice were enucleated and imbedded in optimal cutting temperature to perform histological and immunofluorescence analysis at the end of a 4-week observation period.
  
  
  RESULTS
  Four weeks after the siRNA-PKCα injections, there are 100% lens dissolution and 100% PVR in the 250 nM group and 70%, 70%, 70%, and 50% PVR in the 500 nM, 750 nM, 1000 nM, and 1500 nM groups, respectively, which is significantly different from the negative group. Abnormalities in fundus appearance were related to the concentrations of siRNA-PKCα; a higher concentration of siRNA-PKCα resulted in a more normal fundus. Histological sections by hematoxylin-eosin staining of the eyes support the clinical observation. Immunofluorescence analysis showed that RPE65, glutamine synthase, glial acidic fibrillary protein, and α-smooth muscle actin were increasing in the retina with the decreasing concentration of siRNA-PKCα, indicating that intraocular siRNA-PKCα can partly inhibit changes of markers for glia cells, fibroblast cells, retinal pigment epithelium cells, and Müller cells in the process of PVR.
  
  
  CONCLUSIONS
  Gene therapy with siRNA-PKCα could effectively inhibit PVR in mice and provide us with a novel therapeutic target on PVR.
authors.affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, People's Republic of China. gaoqy@mail.sysu.edu.cn
authors.forename: Qianying
authors.initials: Q
authors.lastname: Gao
authors.forename: Wencong
authors.initials: W
authors.lastname: Wang
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Lan
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Yang
authors.forename: Yongguang
authors.initials: Y
authors.lastname: Yuan
authors.forename: Juan
authors.initials: J
authors.lastname: Tan
authors.forename: Yao
authors.initials: Y
authors.lastname: Zong
authors.forename: Zhaoxin
authors.initials: Z
authors.lastname: Jiang
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D010450:Endopeptidases; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D016133:Polymerase Chain Reaction; D051571:Protein Kinase C-alpha / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000235:genetics / Q000378:metabolism; D012160:Retina / Q000737:chemistry / Q000378:metabolism / Q000473:pathology; D014162:Transfection / Q000379:methods; D018630:Vitreoretinopathy, Proliferative / Q000139:chemically induced / Q000235:genetics / Q000473:pathology / Q000628:therapy*
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering; D051571:Protein Kinase C-alpha; D010450:Endopeptidases; C023139:dispase
keywords: dispase; proliferative vitreoretinopathy; protein kinase Cα; small interference RNA