title: Enzymatic electrochemical detection coupled to multivariate calibration for the determination of phenolic compounds in environmental samples.
abstract: An approach based on the electrochemical detection of the horseradish peroxidase enzymatic reaction by means of square wave voltammetry was developed for the determination of phenolic compounds in environmental samples. First, a systematic optimization procedure of three factors involved in the enzymatic reaction was carried out using response surface methodology through a central composite design. Second, the enzymatic electrochemical detection coupled with a multivariate calibration method based in the partial least-squares technique was optimized for the determination of a mixture of five phenolic compounds, i.e. phenol, p-aminophenol, p-chlorophenol, hydroquinone and pyrocatechol. The calibration and validation sets were built and assessed. In the calibration model, the LODs for phenolic compounds oscillated from 0.6 to 1.4 × 10(-6) mol L(-1). Recoveries for prediction samples were higher than 85%. These compounds were analyzed simultaneously in spiked samples and in water samples collected close to tanneries and landfills.
authors.affiliation: Laboratorio de Sensores y Biosensores, Cátedra de Química Analítica I, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, 3000 Santa Fe, Argentina. shernand@fbcb.unl.edu.ar
authors.forename: Silvia R
authors.initials: SR
authors.lastname: Hernandez
authors.forename: Silvina V
authors.initials: SV
authors.lastname: Kergaravat
authors.forename: Maria Isabel
authors.initials: MI
authors.lastname: Pividori
mesh_terms: D000627:Aminophenols / Q000032:analysis*; D002138:Calibration; D002396:Catechols / Q000032:analysis*; D002733:Chlorophenols / Q000032:analysis*; D055664:Electrochemical Techniques; D005163:Factor Analysis, Statistical; D006735:Horseradish Peroxidase / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D006873:Hydroquinones / Q000032:analysis*; D057230:Limit of Detection; D019800:Phenol / Q000032:analysis*; D014867:Water / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D000627:Aminophenols; D002396:Catechols; D002733:Chlorophenols; D006873:Hydroquinones; D014874:Water Pollutants, Chemical; D014867:Water; D019800:Phenol; C029107:4-chlorophenol; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; C034221:catechol; C026729:4-aminophenol; C031927:hydroquinone