title: [Effect of subchronic realgar exposure on Glu and Gln in infant rat brain].
abstract: OBJECTIVE
  To study the effect of realgar on Glu and Gln on rat brain tissues.
  
  
  METHODS
  Forty-eight Wistar rats were divided into 4 groups randomly:control group,low dosage group, moderate dosage group and high dosage group. The treatment groups were treated with realgar by gastric perfusion at a dosage of 0.3 g/kg, 0.9 g/kg, 2.7 g/kg and the control group ones were orally given the same volume of 0.5% sodium carboxymethylcellulose (CMC-Na) for 6 weeks. The contents of inorganic arsenic, monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA) in brain tissues were measured by hydride generation-atomic absorption (HG-AAS) method. The contents of amino acid neurotransmitters in brain tissues of rats were determined by means of high performance liquid chromatography with precolumn derivatization.
  
  
  RESULTS
  The levels of MMA and DMA in brain increased as the dosage of realgar increased, while the second methylation index declined. Compared with control group,the levels of Glu was significantly decreased in realgar treated group (P < 0.05); Gln also tended to decrease and that of low dosage group was obviously decreased compared with controls.
  
  
  CONCLUSIONS
  Realgar exposure can cause accumulation of MMA and DMA,declination of second methylation index and the reduction of Glu and Gln in brain tissue.
authors.affiliation: College of Public Health, China Medical University, Shenyang 110001, China. changpeipei2000@yahoo.com.cn
authors.forename: Bei
authors.initials: B
authors.lastname: Chang
authors.forename: Tao-Guang
authors.initials: TG
authors.lastname: Huo
authors.forename: Ying-Hua
authors.initials: YH
authors.lastname: Zhang
authors.forename: Wei-Kai
authors.initials: WK
authors.lastname: Li
authors.forename: Hui-Lei
authors.initials: HL
authors.lastname: Yang
authors.forename: Hong
authors.initials: H
authors.lastname: Jiang
authors.forename: Gui-Fan
authors.initials: GF
authors.lastname: Sun
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001151:Arsenic / Q000378:metabolism* / Q000633:toxicity; D020261:Arsenic Poisoning; D001152:Arsenicals / Q000008:administration & dosage* / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D002101:Cacodylic Acid / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D005260:Female; D018698:Glutamic Acid / Q000378:metabolism*; D005973:Glutamine / Q000378:metabolism*; D008297:Male; D008745:Methylation; D051381:Rats; D013440:Sulfides / Q000008:administration & dosage* / Q000633:toxicity
chemical_list: D001152:Arsenicals; D013440:Sulfides; D005973:Glutamine; D018698:Glutamic Acid; C058317:arsenic disulfide; D002101:Cacodylic Acid; C020300:monomethylarsonic acid; D001151:Arsenic