title: Two novel furostanol saponins from the tubers of Ophiopogon japonicus.
abstract: Phytochemical investigation of the fresh tubers of Ophiopogon japonicus led to the isolation of two new furostanol saponins (1 and 2) together with two known steroidal saponins (3 and 4). Comprehensive spectroscopic analysis allowed the chemical structures of two new compounds to be elucidated as (25R)-26-O-[β-d-glucopyranosyl-(1 → 2)-β-d-glucopyranosyl]-5-ene-furost-1β,3β,22α,26-tetraol-3-O-α-l-rhamnopyranosyl-(1 → 2)-[β-d-xylopyranosyl-(1 → 4)]-β-d-glucopyranoside (1, ophiopogonin P) and (25R)-26-O-[β-d-glucopyranosyl-(1 → 6)-β-d-glucopyranosyl]-5-ene-furost-1β,3β,22α,26-tetraol-3-O-α-l-rhamnopyranosyl-(1 → 2)-[β-d-xylopyranosyl-(1 → 4)]-β-d-glucopyranoside (2, ophiopogonin Q). Furostanol saponins with the disaccharide chain linked at C-26 hydroxy group of the aglycone have been rarely reported from natural sources.
authors.affiliation: Learning Key Laboratory for Pharmacoproteomics of Hunan Province, Institute of Pharmacy and Pharmacology, University of South China, Hengyang 421001, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Guo
authors.forename: Yi-Xun
authors.initials: YX
authors.lastname: Liu
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Kang
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.forename: He-Shui
authors.initials: HS
authors.lastname: Yu
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhao
authors.forename: Cheng-Qi
authors.initials: CQ
authors.lastname: Xiong
authors.forename: Bai-Ping
authors.initials: BP
authors.lastname: Ma
mesh_terms: D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D031441:Ophiopogon / Q000737:chemistry*; D035281:Plant Tubers / Q000737:chemistry; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification*; D013237:Stereoisomerism; D013261:Sterols / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D004365:Drugs, Chinese Herbal; D012503:Saponins; D013261:Sterols; C582493:ophiopogonin P; C582472:ophiopogonin Q