title: Chemical structure and in vitro antitumor activity of rhamnolipids from Pseudomonas aeruginosa BN10.
abstract: A newly isolated indigenous strain BN10 identified as Pseudomonas aeruginosa was found to produce glycolipid (i.e., rhamnolipid-type) biosurfactants. Two representative rhamnolipidic fractions, RL-1 and RL-2, were separated on silica gel columns and their chemical structure was elucidated by a combination of nuclear magnetic resonance and mass spectroscopy. Subsequently, their cytotoxic effect on cancer cell lines HL-60, BV-173, SKW-3, and JMSU-1 was investigated. RL-1 was superior in terms of potency, causing 50 % inhibition of cellular viability at lower concentrations, as compared to RL-2. Furthermore, the results from fluorescent staining analysis demonstrated that RL-1 inhibited proliferation of BV-173 pre-B human leukemia cells by induction of apoptotic cell death. These findings suggest that RL-1 could be of potential for application in biomedicine as a new and promising therapeutic agent.
authors.affiliation: Institute of Microbiology, Bulgarian Academy of Sciences, Acad. G. Bonchev str., bl 26, 1113 Sofia, Bulgaria.
authors.forename: Neli
authors.initials: N
authors.lastname: Christova
authors.forename: Boryana
authors.initials: B
authors.lastname: Tuleva
authors.forename: Anton
authors.initials: A
authors.lastname: Kril
authors.forename: Milka
authors.initials: M
authors.lastname: Georgieva
authors.forename: Spiro
authors.initials: S
authors.lastname: Konstantinov
authors.forename: Ivan
authors.initials: I
authors.lastname: Terziyski
authors.forename: Biliana
authors.initials: B
authors.lastname: Nikolova
authors.forename: Ivanka
authors.initials: I
authors.lastname: Stoineva
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000494:pharmacology; D041702:BALB 3T3 Cells / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival; D006017:Glycolipids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D011550:Pseudomonas aeruginosa / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013501:Surface-Active Agents / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D000970:Antineoplastic Agents; D006017:Glycolipids; D013501:Surface-Active Agents; C418382:rhamnolipid; C583946:rhamnolipid-1, Pseudomonas aeruginosa