title: Factors influencing the deposition of hydroxyapatite coating onto hollow glass microspheres.
abstract: Hydroxyapatite (HA) and HA coated microcarriers for cell culture and delivery have attracted more attention recently, owing to the rapid progress in the field of tissue engineering. In this research, a dense and uniform HA coating with the thickness of about 2 μm was successfully deposited on hollow glass microspheres (HGM) by biomimetic process. The influences of SBF concentration, immersion time, solid/liquid ratio and activation of HGM on the deposition rate and coating characteristics were discussed. X-ray diffraction (XRD) and Fourier transform infrared spectrum (FTIR) analyses revealed that the deposited HA is poorly crystalline. The thickness of HA coating showed almost no increase after immersion in 1.5SBF for more than 15 days with the solid/liquid ratio of 1:150. At the same time, SBF concentration, solid/liquid ratio and activation treatment played vital roles in the formation of HA coating on HGM. This poorly crystallized HA coated HGM could have potential use as microcarrier for cell culture.
authors.affiliation: Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials, Ministry of Education, Shandong University, Ji'nan 250061, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jiao
authors.forename: Gui-Yong
authors.initials: GY
authors.lastname: Xiao
authors.forename: Wen-Hua
authors.initials: WH
authors.lastname: Xu
authors.forename: Rui-Fu
authors.initials: RF
authors.lastname: Zhu
authors.forename: Yu-Peng
authors.initials: YP
authors.lastname: Lu
mesh_terms: D020099:Coated Materials, Biocompatible; D017886:Durapatite / Q000737:chemistry*; D005898:Glass; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D014961:X-Ray Diffraction
chemical_list: D020099:Coated Materials, Biocompatible; D017886:Durapatite