title: Lignin degradation by selected fungal species.
abstract: As biological decomposition of plant biomass represents a popular alternative environmental-friendly and economically justified process, screening of ligninolytic enzyme systems of various fungal species is a topical study area. The goal of the study was to obtain clear insight into the dynamics of laccase, Mn-dependent peroxidase, and Mn-independent peroxidase activity and levels of wheat straw lignin degradation in seven wood-rotting fungi. The best laccase producers were Pleurotus ostreatus and Pleurotus eryngii. Lenzites betulinus and Fomitopsis pinicola were the best Mn-dependent peroxidase producers, and P. ostreatus the weakest one. The peak of Mn-independent peroxidase was noted in Dichomytus squalens, and the minimum value in P. ostreatus. The profiles of the three enzymes, obtained by isoelectric focusing, were variable depending on the species and cultivation period. D. squalens was the best lignin degrader (34.1% of total lignin amount), and P. ostreatus and P. eryngii the weakest ones (7.1% and 14.5%, respectively).
authors.affiliation: University of Belgrade, Faculty of Biology, Takovska 43, 11000 Belgrade, Serbia. knezevica@bio.bg.ac.rs
authors.forename: Aleksandar
authors.initials: A
authors.lastname: Knežević
authors.forename: Ivan
authors.initials: I
authors.lastname: Milovanović
authors.forename: Mirjana
authors.initials: M
authors.lastname: Stajić
authors.forename: Nikola
authors.initials: N
authors.lastname: Lončar
authors.forename: Ilija
authors.initials: I
authors.lastname: Brčeski
authors.forename: Jelena
authors.initials: J
authors.lastname: Vukojević
authors.forename: Jasmina
authors.initials: J
authors.lastname: Cilerdžić
mesh_terms: D001673:Biodegradation, Environmental; D005285:Fermentation; D005658:Fungi / Q000201:enzymology / Q000378:metabolism*; D007525:Isoelectric Focusing; D042845:Laccase / Q000378:metabolism; D008031:Lignin / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism; D014908:Triticum / Q000378:metabolism
chemical_list: D008031:Lignin; D042845:Laccase; D010544:Peroxidases; C051129:manganese peroxidase