title: Regulation of conjugated hemoglobin on micelles through copolymer chain sequences and the protein's isoelectric aggregation.
abstract: Amphiphilic triblock copolymers with carbonyl groups located either in the middle segment or in the third side block are synthesized by adjusting feeding sequence of the comonomers. The conjugation of hemoglobin (Hb) on the copolymer micelles is realized by condensation reactions of carbonyl with the amino groups of Hb, and the gas-binding capacity of Hb is well preserved. Interestingly, the reassembly behavior of Hb-conjugated micelles (HbM) is explored by adjusting the pH. As for triblock copolymers with a carbonyl-functionalized segment as the third block, Hb is rearranged into the inner core of micelles when the pH is adjusted close to the isoelectric point of Hb. Therefore, it may provide a new needed route for fabrication of protein carriers, which is different from the traditional encapsulation technique.
authors.affiliation: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Taihang
authors.initials: T
authors.lastname: Li
authors.forename: Gao
authors.initials: G
authors.lastname: Chen
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Li
authors.forename: Lesan
authors.initials: L
authors.lastname: Yan
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Xie
authors.forename: Xiabin
authors.initials: X
authors.lastname: Jing
authors.forename: Yubin
authors.initials: Y
authors.lastname: Huang
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D002460:Cell Line; D004337:Drug Carriers / Q000737:chemistry* / Q000627:therapeutic use; D006454:Hemoglobins / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D008823:Micelles; D019906:Nuclear Magnetic Resonance, Biomolecular; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry* / Q000627:therapeutic use
chemical_list: D001672:Biocompatible Materials; D004337:Drug Carriers; D006454:Hemoglobins; D008823:Micelles; D011108:Polymers; D011506:Proteins; D011092:Polyethylene Glycols
keywords: biocompatibility; chain sequences; hemoglobin; isoelectric aggregation; micelles