title: Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry for Identification of In Vitro and In Vivo Metabolites of Bornyl Gallate in Rats.
abstract: Bornyl gallate (BG) is a potential drug candidate synthesized by the reaction of two natural products, gallic acid and borneol. Previous studies have strongly suggested that BG is worthy of further investigation due to antioxidant, antiatherosclerosis activities, and obvious activity of stimulating intersegmental vessel growth in zebrafish. This work was designed to elucidate the metabolic profile of BG through analyzing its metabolites in vitro and in vivo by a chromatographic separation coupled with a mass spectrometry. The metabolites of BG were characterized from the rat liver microsome incubation solution, as well as rat urine and plasma after oral administration. Chromatographic separation was performed on an Agilent TC-C18 column (250 mm × 4.6 mm, 5  μ m) with gradient elution using methanol and water containing 0.2% (V : V) formic acid as the mobile phase. Metabolites identification involved analyzing the retention behaviors, changes of molecular weights and MS/MS fragment patterns of BG and the metabolites. Five compounds were identified as isomers of hydroxylated BG metabolites in vitro. The major metabolites of BG in rat urine and plasma proved to be BG-O-glucuronide and O-methyl BG-O-glucuronide. The proposed method confirmed to be a reliable and sensitive alternative for characterizing metabolic pathways of BG.
authors.affiliation: College of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Lan
authors.forename: Liujiao
authors.initials: L
authors.lastname: Bian
authors.forename: Xinfeng
authors.initials: X
authors.lastname: Zhao
authors.forename: Pu
authors.initials: P
authors.lastname: Jia
authors.forename: Xue
authors.initials: X
authors.lastname: Meng
authors.forename: Yizhen
authors.initials: Y
authors.lastname: Wu
authors.forename: Shixiang
authors.initials: S
authors.lastname: Wang
authors.forename: Sha
authors.initials: S
authors.lastname: Liao
authors.forename: Jie
authors.initials: J
authors.lastname: Yu
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zheng