title: Multiplexed tyrosine kinase activity detection in cancer cells using a hydrogel immobilized substrate.
abstract: Kinases play a key role in cellular signaling, and the overactivation or overexpression of these kinases has been linked to a variety of cancers. Tyrosine kinase inhibitors treat the mechanism of these cancers by targeting the specific kinases that are overactive. Some patients, however, do not respond to these inhibitors or develop resistance to these inhibitors during treatment. Additionally, even within cancers of the same tissue type, different kinases may be overactive in different patients. For example, some lung cancers overexpress epidermal growth factor receptor (EGFR) and respond to EGFR inhibitors, whereas other lung cancers do not overexpress EGFR and receive no benefit from this treatment. Even among patients exhibiting EGFR overexpression, some do not respond to EGFR kinase inhibitors because other kinases, such as Met kinase, are also overactivated. Here we describe a quantitative and specific multiplexed microfluidic assay using a hydrogel immobilized substrate for measuring the kinase activity of Met and Abl kinase from cancer cells. We immobilized kinase-specific substrates on macroporous hydrogel micropillars in microchannels. These microchannels were incubated with 6 μl of a kinase reaction solution containing cancer cell lysate, and we measured kinase activity via fluorescence detection of a phosphotyrosine antibody. We showed that the assay can specifically measure the activity of both Met and Abl kinase within one microchannel and has the potential to measure the activity of as many as five kinases within one microchannel. The assay also detected Met kinase inhibition from lysates of cancer cells grown in the Met kinase inhibitor PHA665752.
authors.affiliation: Department of Chemical and Biological Engineering, University of Wisconsin-Madison, 1415 Engineering Drive, Madison, WI 53706, USA.
authors.forename: Alicia D
authors.initials: AD
authors.lastname: Powers
authors.forename: Wenquing
authors.initials: W
authors.lastname: Han
authors.forename: Bi
authors.initials: B
authors.lastname: Liu
authors.forename: Sean P
authors.initials: SP
authors.lastname: Palecek
mesh_terms: D045744:Cell Line, Tumor; D066246:ErbB Receptors / Q000032:analysis; D005453:Fluorescence; D051417:Focal Adhesion Kinase 1 / Q000032:analysis; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D007211:Indoles / Q000494:pharmacology; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D016374:Oncogene Proteins v-abl / Q000032:analysis; D019000:Phosphotyrosine / Q000032:analysis; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D011505:Protein-Tyrosine Kinases / Q000032:analysis*; D019859:Proto-Oncogene Proteins c-met / Q000032:analysis / Q000037:antagonists & inhibitors; D012996:Solutions; D013450:Sulfones / Q000494:pharmacology
chemical_list: C480541:5-((2,6-dichlorobenzyl)sulfonyl)-3-((3,5-dimethyl-4-((2-(pyrrolidin-1-ylmethyl)pyrrolidin-1-yl)carbonyl)-1H-pyrrol-2-yl)methylene)-1,3-dihydro-2H-indol-2-one; D007211:Indoles; D016374:Oncogene Proteins v-abl; D047428:Protein Kinase Inhibitors; D012996:Solutions; D013450:Sulfones; D019000:Phosphotyrosine; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; C512478:EGFR protein, human; D066246:ErbB Receptors; C491743:MET protein, human; D011505:Protein-Tyrosine Kinases; D019859:Proto-Oncogene Proteins c-met; D051417:Focal Adhesion Kinase 1; C496050:PTK2 protein, human