title: Biosynthesis and preparation of T-2 Toxin, HT-2 Toxin and Neosolantol.
abstract: Biosynthesis of trichothecenes by strains ofF. sporotriahioides KF 9 6 and KF 530 was performed on rice as a medium. Three toxins in significant amounts were produced, with yield: T-2 toxin 0.7g from 600g of drv culture, being the highest of the three metabolites, HT-2 toxin 0.06g an Neosolaniol 0.015g. Toxins were extracted with methanol from ground and defatted dry culture. Liquid/liquid partition, using chloroform/water saturated solution of sodium bicarbonate as a preliminary purification was applied. Silicagel and charcoal columns were used in further purification. Toxin were separated by preparative TLC and crystallized from methanol.
authors.affiliation: Department of Chemistry, Agricultural University, ul. Wojska Polskiego 75, 60-625, Poznań, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Kostecki
authors.forename: H
authors.initials: H
authors.lastname: Jeleń
authors.forename: P
authors.initials: P
authors.lastname: Gliński
authors.forename: J
authors.initials: J
authors.lastname: Chełkowski