title: Polymerase chain reaction with insertion sequence-specific and -unrelated primers: a new tool for the identification of IS1548 insertion targets in Streptococcus agalactiae.
abstract: We developed a PCR method with outward insertion sequence-specific and -unrelated primers to identify IS1548 targets in the genome of unsequenced Streptococcus agalactiae strains. Our rapid and easy method allowed the identification of previously known but also of yet unnoticed integration sites in the three clinical isolates tested.
authors.affiliation: Équipe "Bactéries et Risque Materno-Foetal", UMR1282 Infectiologie et Santé Publique, Université de Tours, F-37032 Tours, France. maud.flechard@voila.fr
authors.forename: Maud
authors.initials: M
authors.lastname: Fléchard
authors.forename: Agnès
authors.initials: A
authors.lastname: Rosenau
authors.forename: Laurent
authors.initials: L
authors.lastname: Mereghetti
authors.forename: Philippe
authors.initials: P
authors.lastname: Gilot
mesh_terms: D017931:DNA Primers / Q000235:genetics*; D004251:DNA Transposable Elements; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D005827:Genetics, Microbial / Q000379:methods*; D006801:Humans; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D013290:Streptococcal Infections / Q000382:microbiology; D013292:Streptococcus agalactiae / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D017931:DNA Primers; D004251:DNA Transposable Elements; D004269:DNA, Bacterial