title: Tissue preparation for MALDI-MS imaging of protein and peptides.
abstract: Matrix-assisted laser desorption/ionization (MALDI) imaging is rapidly gaining importance in the -biomarkers field because it is able to detect several analytes at the same time and to assign to each one of them not only an m/z value but also spatial distribution. Here we present the detailed description of sample preparation for protein and peptide MALDI imaging assays. This chapter describes the microtomy performed in a cryostat to produce tissue slides mounted onto glass slides suitable for MALDI. Sample preparation will include matrix coating procedure with a sensor-controlled aerosol. Finally, we will show some examples of how data can be visualized to suit the purposes of the research.
authors.affiliation: Protein Chemistry Laboratory, Advance Technology Program, SAIC-Frederick, Frederick National Laboratory for Cancer Research, Frederick, MD, USA.
authors.forename: Simona
authors.initials: S
authors.lastname: Colantonio
authors.forename: Roberta M
authors.initials: RM
authors.lastname: Smith
mesh_terms: D000595:Amino Acid Sequence; D006801:Humans; D008867:Microtomy; D010455:Peptides / Q000032:analysis* / Q000737:chemistry; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D013048:Specimen Handling / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D010455:Peptides; D011506:Proteins