title: Rinsing of allograft bone does not improve implant fixation: a study in 12 dogs.
abstract: OBJECTIVE
  Impacted morselized allograft bone is a well-established method for reconstructing bone defects at revision surgery. However, the incorporation of bone graft is not always complete, and a substantial volume of fibrous tissue has been found around grafted implants. We hypothesized that rinsing the bone graft may improve graft incorporation by removing the majority of immunogenic factors present in blood, marrow, and fat.
  
  
  METHODS
  We implanted a cylindrical (10- × 6-mm) porous-coated Ti implant into each proximal tibia of 12 dogs. The implants were surrounded by a 2.5-mm gap into which morselized fresh frozen allograft bone was impacted. The bone graft was either (1) untreated or (2) rinsed in 37°C saline for 3 × 1 min. After 4 weeks, the animals were killed and implant fixation was evaluated by mechanical push-out and histomorphometry.
  
  
  RESULTS
  The groups (rinsed vs. control) were similar regarding mechanical implant fixation (mean (SD)): shear strength (MPa) 2.7 (1.0) vs. 2.9 (1.2), stiffness (MPa/mm) 15 (6.7) vs. 15 (5.6), and energy absorption (kJ/m(2)) 0.5 (0.2) vs. 0.6 (0.4), The same was evident for the new bone formation on the implant surface and around the implant: ongrowth (%) 6 vs. 7 and ingrowth (%) 9 vs. 9. Although not statistically significant, a 61% reduction in fibrous tissue ongrowth and 50% reduction in ingrowth were found in the rinsed group.
  
  
  CONCLUSIONS
  Within the limits of this experimental model, we did not detect any benefits of rinsing morselized allograft bone prior to impaction grafting.
authors.affiliation: Orthopaedic Research Laboratory, Aarhus University Hospital, Denmark. jeppe.barckman@ki.au.dk
authors.forename: Jeppe
authors.initials: J
authors.lastname: Barckman
authors.forename: Jorgen
authors.initials: J
authors.lastname: Baas
authors.forename: Mette
authors.initials: M
authors.lastname: Sørensen
authors.forename: Joan E
authors.initials: JE
authors.lastname: Bechtold
authors.forename: Kjeld
authors.initials: K
authors.lastname: Søballe
mesh_terms: D000818:Animals; D016025:Bone Transplantation / Q000379:methods*; D020099:Coated Materials, Biocompatible; D004285:Dogs; D005260:Female; D020007:Implants, Experimental; D016348:Osseointegration; D011475:Prosthesis Failure; D012086:Reoperation / Q000379:methods; D033081:Shear Strength; D013314:Stress, Mechanical; D007507:Therapeutic Irrigation / Q000379:methods; D013977:Tibia / Q000473:pathology / Q000601:surgery; D016474:Weight-Bearing
chemical_list: D020099:Coated Materials, Biocompatible