title: A fluorescence ratiometric sensor for hypochlorite based on a novel dual-fluorophore response approach.
abstract: A fluorescence ratiometric sensor for OCl(-) has been developed based on a novel dual fluorophore response approach. The sensor molecule contains a coumarin fluorophore and a rhodamine fluorophore, and the two fluorophores are directly linked to an OCl(-) recognition group. The structure of the sensor was characterized by ESI-MS, NMR, and X-ray crystallographic analysis. Upon treatment with OCl(-), both fluorophores in the sensor responded simultaneously at two separate optical windows, with large enhancement of the fluorescence ratio (I578/I501) from 0.01 to 39.55. The fluorescence ratios for the sensor showed a good linearity with the concentration of OCl(-) in the range of 0.2-40 μM and the detection limits is 0.024 μM (SN(-1)=3). Investigation of reaction products indicated that the sensor reaction with OCl(-) produced two new fluorescent molecules, which were responsible for the fluorescence changes in two optical windows. In addition, the sensor showed high selectivity to OCl(-) over other reactive oxygen species, reactive nitrogen species, cations, and anions. The sensor has also been successfully applied to detection of OCl(-) in natural water samples with satisfactory recovery.
authors.affiliation: Functional Molecular Materials Research Centre, Scientific Research Academy & School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, PR China. Linglianglong@gmail.com
authors.forename: Lingliang
authors.initials: L
authors.lastname: Long
authors.forename: Dongdong
authors.initials: D
authors.lastname: Zhang
authors.forename: Xiufen
authors.initials: X
authors.lastname: Li
authors.forename: Jinfang
authors.initials: J
authors.lastname: Zhang
authors.forename: Chi
authors.initials: C
authors.lastname: Zhang
authors.forename: Liping
authors.initials: L
authors.lastname: Zhou
mesh_terms: D003374:Coumarins / Q000138:chemical synthesis* / Q000737:chemistry; D018360:Crystallography, X-Ray; D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D006997:Hypochlorous Acid / Q000032:analysis*; D008968:Molecular Conformation; D012235:Rhodamines / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D013141:Spiro Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D003374:Coumarins; D005456:Fluorescent Dyes; D012235:Rhodamines; D013141:Spiro Compounds; D014867:Water; D006997:Hypochlorous Acid