title: [Hepatitis A virus mimotope mapping by phage display peptide library].
abstract: OBJECTIVE
  A 12 mer phage display peptide library was used to identify hepatitis A virus mimotopes of antigenic determinants, to provide the feasibility of virus epitope mapping by using this approach.
  
  
  METHODS
  Using purified anti-hepatitis A virus monoclonal antibody as affinity selective molecule, phage display peptide library was biopanned and positive clones were selected by ELISA, competition assay and DNA sequencing.
  
  
  RESULTS
  10 ELISA positive clones were chosen for DNA sequencing, and the displayed peptide sequences were deduced. 9 of them showed identical nucleotide sequence, and similarity in their amino acid sequence with VP1 of HAV HM175 was found, but no sequence homology was found between the other phage clone and the capsid proteins of HAV. Those peptides may behave as mimotopes of HAV.
  
  
  CONCLUSIONS
  The mimotope of HAV was selected by using phage display peptide library screening. The results provide the potential of this method to search for the mimotopes of the virus.
authors.affiliation: Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
authors.forename: Jing-Yuan
authors.initials: JY
authors.lastname: Cao
authors.forename: Jian-Dong
authors.initials: JD
authors.lastname: Li
authors.forename: Hui-Hui
authors.initials: HH
authors.lastname: Zheng
authors.forename: Sheng-Li
authors.initials: SL
authors.lastname: Bi
authors.forename: De-Xin
authors.initials: DX
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000956:Antigens, Viral / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D018604:Epitope Mapping; D000939:Epitopes; D006506:Hepatitis A / Q000821:virology; D030041:Hepatitis A virus / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D019151:Peptide Library
chemical_list: D000956:Antigens, Viral; D000939:Epitopes; D019151:Peptide Library