title: In vitro induction of tumor necrosis factor-α by ochratoxin A (OTA) from rat liver: role of Kupffer cells.
abstract: Tumor necrosis factor-α (TNF-α) is released from blood-free perfused rat liver by the fungal metabolite ochratoxin A. Here we have identified Kupffer cells as the sole source of OTA-mediated cytokine release. If single cell preparation of Kupffer cells, hepatocytes, or sinusoidal endothelial cells were prepared from rat livers, only Kupffer cells released TNF-α upon incubation with 2.5 μmol/l OTA. OTA failed to induce TNF-α release in the blood-free perfused isolated rat liver when Kupffer cells were blockedin vitro by 15 μmol/l gadolinium chloride. When rats were pretreatedin vivo with the Kupffer cell depleting clodronate liposomes, OTA-mediated TNF-α release was abrogated in the isolated perfused liver model.
authors.affiliation: Faculty of Veterinary Medicine, Institute of Pharmacology and Toxicology, Justus-Liebig-University Giessen, Frankfurters Strasse 107, 35392, Giessen, Germany.
authors.forename: L
authors.initials: L
authors.lastname: Al-Anati
authors.forename: R
authors.initials: R
authors.lastname: Reinehr
authors.forename: N
authors.initials: N
authors.lastname: Van Rooijen
authors.forename: E
authors.initials: E
authors.lastname: Petzinger