title: Structural studies of an immunoenhancing glucan of an ectomycorrhizal fungus Ramaria botrytis.
abstract: A water-soluble glucan was isolated from the alkaline extract of an ectomycorrhizal fungus, Ramaria botrytis. On the basis of sugar analysis, methylation analysis, Smith degradation, partial hydrolysis, and 1D/2D NMR studies, the structure of the repeating unit of the glucan was established as: [structure: see text]. This glucan showed immunostimulating activity by NO production on RAW 264.7, a murine macrophage cell line. Splenocyte and thymocyte proliferation were measured using, respectively, single cell suspensions of spleen and thymus obtained from normal mice.
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Sunil K
authors.initials: SK
authors.lastname: Bhanja
authors.forename: Dilip
authors.initials: D
authors.lastname: Rout
authors.forename: Pradip
authors.initials: P
authors.lastname: Patra
authors.forename: Chanchal K
authors.initials: CK
authors.lastname: Nandan
authors.forename: Birendra
authors.initials: B
authors.lastname: Behera
authors.forename: Tapas K
authors.initials: TK
authors.lastname: Maiti
authors.forename: Syed S
authors.initials: SS
authors.lastname: Islam
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry* / Q000494:pharmacology; D000818:Animals; D001487:Basidiomycota / Q000737:chemistry*; D002236:Carbohydrate Conformation; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005936:Glucans / Q000737:chemistry* / Q000276:immunology / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D051379:Mice; D009569:Nitric Oxide / Q000096:biosynthesis; D019906:Nuclear Magnetic Resonance, Biomolecular; D013154:Spleen / Q000166:cytology / Q000187:drug effects; D013329:Structure-Activity Relationship; D060168:Thymocytes / Q000166:cytology / Q000187:drug effects
chemical_list: D000276:Adjuvants, Immunologic; D005936:Glucans; D009569:Nitric Oxide