title: Bacillus thermotolerans sp. nov., a thermophilic bacterium capable of reducing humus.
abstract: A novel thermotolerant bacterium, designated SgZ-8(T), was isolated from a compost sample. Cells were non-motile, endospore-forming, Gram-staining positive, oxidase-negative and catalase-positive. The isolate was able to grow at 20-65 °C (optimum 50 °C) and pH 6.0-9.0 (optimum 6.5-7.0), and tolerate up to 9.0 % NaCl (w/v) under aerobic conditions. Anaerobic growth occurred with anthraquinone-2,6-disulphonate (AQDS), fumarate and NO3(-) as electron acceptors. Phylogenetic analysis based on the16S rRNA and gyrB genes grouped strain SgZ-8(T) into the genus Bacillus, with the highest similarity to Bacillus badius JCM 12228(T) (96.2 % for 16S rRNA gene sequence and 83.5 % for gyrB gene sequence) among all recognized species in the genus Bacillus. The G+C content of the genomic DNA was 49.3 mol%. The major isoprenoid quinone was menaquinone 7 (MK-7) and the polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid. The major cellular fatty acid was iso-C16 : 0. On the basis of its phenotypic and phylogenetic properties, chemotaxonomic analysis and the results of physiological and biochemical tests, strain SgZ-8(T) ( = CCTCC AB 2012108(T) = KACC 16706(T)) was designated the type strain of a novel species of the genus Bacillus, for which the name Bacillus thermotolerans sp. nov. is proposed.
authors.affiliation: Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, PR China.
authors.forename: Guiqin
authors.initials: G
authors.lastname: Yang
authors.affiliation: 101 Research Institute of the Ministry of Civil Affairs, Beijing 100070, PR China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, PR China.
authors.forename: Shungui
authors.initials: S
authors.lastname: Zhou
authors.affiliation: 101 Research Institute of the Ministry of Civil Affairs, Beijing 100070, PR China.
authors.forename: Dehui
authors.initials: D
authors.lastname: Yang
authors.affiliation: Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, PR China.
authors.forename: Yueqiang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, PR China.
authors.forename: Dingmei
authors.initials: D
authors.lastname: Wang
mesh_terms: D000880:Anthraquinones / Q000378:metabolism; D001407:Bacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D005650:Fumarates / Q000378:metabolism; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012987:Soil; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives
chemical_list: D000880:Anthraquinones; D004269:DNA, Bacterial; D005227:Fatty Acids; D005650:Fumarates; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012987:Soil; C408124:anthraquinone-2,6-disulfonate; D024482:Vitamin K 2; C062629:menaquinone 7