title: Influence of genistein on c-Jun, c-Fos and Fos-B of AP-1 subunits expression in skin keratinocytes, fibroblasts and keloid fibroblasts cultured in vitro.
abstract: Genistein is a well known flavonoid that exhibits antioxidant, antiproliferative, proapoptotic, antiangiogenic, as well as estrogenic and anti-estrogenic activity. Extensive studies in the field of dermatology and cosmetology in recent years revealed that genistein is a promising anti-aging, anti-photoaging and anti-carcinogenic agent for skin care. Essential role in skin prematuring aging and carcinogenesis play AP-1 transcription factors that are activated, among others, by environmental factors: ultraviolet light and free radicals. Genistein is a potent antioxidant and inhibitor of AP-1 activity. The aim of the study was to investigate genistein as a potential regulator of C-JUN, C-FOS and FOS-B of AP-1 subunits expression in skin keratinocytes, fibroblasts and keloid fibroblasts cultured in vitro. In presented study, genistein modulated C-JUN expression in epidermal keratinocytes and dermal fibroblasts cultured in vitro. The expression of C-JUN was higher in keratinocytes treated with 37 and 370 microM genistein. In dermal fibroblasts genistein regulated C-JUN expression in dose-dependent manner. The expression of C-JUN was lower in fibroblasts treated with 370 microM genistein and higher in fibroblasts treated with 37 microM genistein. Genistein in 370 microM concentration inhibited C-FOS expression in fibroblasts, whereas in 370 and 37 microM concentration genistein inhibited FOS-B expression in keratinocytes. Furthermore, genistein was able to modulate C-JUN and C-FOS genes expression in keloid fibroblasts cultured in vitro. In these cells, transcriptional activity of C-JUN and C-FOS expression depended on employed concentration of genistein. The expression of C-JUN and C-FOS was higher in keloid fibroblasts treated with 370 microM genistein and lower in keloid fibroblasts treated with 37 microM genistein.
authors.affiliation: Department of Cosmetology, School of Pharmacy, Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, 3 Kasztanowa St., 41-200 Sosnowiec, Poland. magda@sum.edu.pl
authors.forename: Magdalena
authors.initials: M
authors.lastname: Jurzak
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Adamczyk
mesh_terms: D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003879:Dermatologic Agents / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005786:Gene Expression Regulation; D019833:Genistein / Q000494:pharmacology*; D006801:Humans; D007627:Keloid / Q000378:metabolism / Q000473:pathology*; D015603:Keratinocytes / Q000187:drug effects* / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism*; D016755:Proto-Oncogene Proteins c-jun / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D018808:Transcription Factor AP-1 / Q000235:genetics / Q000378:metabolism*
chemical_list: D003879:Dermatologic Agents; C064269:FOSB protein, human; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D012333:RNA, Messenger; D018808:Transcription Factor AP-1; D019833:Genistein