title: Next-generation sequencing of cancer consensus genes in lymphoma.
abstract: Sensitive identification of mutations in genes related to the pathogenesis of cancer is a prerequisite for risk-stratified therapies. Next-generation sequencing (NGS) in lymphoma has revealed genetic heterogeneity which makes clinical translation challenging. We established a 454-based targeted resequencing platform for robust high-throughput sequencing from limited material of patients with lymphoma. Hotspot mutations in the most frequently mutated cancer consensus genes were amplified in a two-step multiplex-polymerase chain reation (PCR) which was optimized for homogeneous coverage of all regions of interest. We show that targeted resequencing based on NGS technologies allows highly sensitive detection of mutations and assessment of clone size. The application of this or similar techniques will help the development of genotype-specific treatment approaches in lymphoma.
authors.affiliation: Department of Translational Oncology, National Center for Tumor Diseases (NCT) and German Cancer Research Center (DKFZ), Heidelberg, Germany.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Hüllein
authors.forename: Alexander
authors.initials: A
authors.lastname: Jethwa
authors.forename: Tatjana
authors.initials: T
authors.lastname: Stolz
authors.forename: Carolin
authors.initials: C
authors.lastname: Blume
authors.forename: Leopold
authors.initials: L
authors.lastname: Sellner
authors.forename: Martin
authors.initials: M
authors.lastname: Sill
authors.forename: Christian
authors.initials: C
authors.lastname: Langer
authors.forename: Anna
authors.initials: A
authors.lastname: Jauch
authors.forename: Anna
authors.initials: A
authors.lastname: Paruzynski
authors.forename: Christof
authors.initials: C
authors.lastname: von Kalle
authors.forename: Manfred
authors.initials: M
authors.lastname: Schmidt
authors.forename: Hanno
authors.initials: H
authors.lastname: Glimm
authors.forename: Thorsten
authors.initials: T
authors.lastname: Zenz
mesh_terms: D019943:Amino Acid Substitution; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D005091:Exons; D005787:Gene Frequency; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D006801:Humans; D008223:Lymphoma / Q000175:diagnosis / Q000235:genetics*; D009154:Mutation; D009857:Oncogenes / Q000235:genetics*