title: High throughput SNP detection system based on magnetic nanoparticles separation.
abstract: Single-nucleotide polymorphism (SNP) was one-base variations in DNA sequence that can often be helpful to find genes associations for hereditary disease, communicable disease and so on. We developed a high throughput SNP detection system based on magnetic nanoparticles (MNPs) separation and dual-color hybridization or single base extension. This system includes a magnetic separation unit for sample separation, three high precision robot arms for pipetting and microtiter plate transferring respectively, an accurate temperature control unit for PCR and DNA hybridization and a high accurate and sensitive optical signal detection unit for fluorescence detection. The cyclooxygenase-2 gene promoter region--65G > C polymorphism locus SNP genotyping experiment for 48 samples from the northern Jiangsu area has been done to verify that if this system can simplify manual operation of the researchers, save time and improve efficiency in SNP genotyping experiments. It can realize sample preparation, target sequence amplification, signal detection and data analysis automatically and can be used in clinical molecule diagnosis and high throughput fluorescence immunological detection and so on.
authors.affiliation: State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.forename: Yingying
authors.initials: Y
authors.lastname: Jia
authors.forename: Man
authors.initials: M
authors.lastname: Ma
authors.forename: Zhiyang
authors.initials: Z
authors.lastname: Li
authors.forename: Hongna
authors.initials: H
authors.lastname: Liu
authors.forename: Song
authors.initials: S
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Deng
authors.forename: Liming
authors.initials: L
authors.lastname: Zhang
authors.forename: Zhuoxuan
authors.initials: Z
authors.lastname: Lu
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Nongyue
authors.initials: N
authors.lastname: He
mesh_terms: D000328:Adult; D001331:Automation; D004247:DNA / Q000302:isolation & purification; D005453:Fluorescence; D060005:Genotyping Techniques; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008832:Microchemistry; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012815:Signal Processing, Computer-Assisted; D013696:Temperature
chemical_list: D058185:Magnetite Nanoparticles; D004247:DNA