title: Deposition of tocopherol and tocotrienol in the tissues of red hybrid tilapia, Oreochromis sp., fed vitamin E-free diets supplemented with different plant oils.
abstract: Vitamin E, a potent antioxidant consisting of four isomers each (α, β, γ, δ) of tocopherol (T) and tocotrienol (T3), is found naturally in plant oils at different concentrations. In this study, four semi-purified isonitrogenous and isolipidic (10 %) diets containing canola oil, cold-pressed soybean oil, wheat germ oil, or palm fatty acid distillates (PFAD) as the sole vitamin E source were fed to triplicate groups of red hybrid tilapia (Oreochromis sp.) fingerlings (14.82 ± 0.05 g) for 45 days. Vitamin E concentrations and composition were measured in the muscle, liver, skin, and adipose tissue. Deposition of α-T (53.4-93.1 % of total vitamin E) predominated over deposition of other isomers, except in the liver of fish fed the SBO diet, where α-T and γ-T deposition was in the ratio 40:60. T3 deposition (2.6-29.4 %) was only detected in tissues of fish fed the PFAD diet; adipose tissue was the major storage depot. Fish fed the SBO diet contained significantly more (P < 0.05) muscle thiobarbituric acid-reactive substances. Muscle fatty acid composition reflected dietary fatty acid profile. This is the first study to compare the deposition in fish tissues of the naturally occurring vitamin E isomers present in plant oils. The type and concentration of endogenous vitamin E and the fatty acid composition of plant oils can affect the oxidative stability of tilapia tissues.
authors.affiliation: Fish Nutrition Laboratory, School of Biological Sciences, Universiti Sains Malyasia, 11800, Penang, Malaysia.
authors.forename: Kuan-Shern
authors.initials: KS
authors.lastname: Lee
authors.forename: Kah-Hay
authors.initials: KH
authors.lastname: Yuen
authors.forename: Wing-Keong
authors.initials: WK
authors.lastname: Ng
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D017756:Aquaculture; D001823:Body Composition; D005227:Fatty Acids / Q000032:analysis; D008099:Liver / Q000378:metabolism; D009132:Muscles / Q000378:metabolism; D010938:Plant Oils; D011897:Random Allocation; D012867:Skin / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D017210:Tilapia / Q000254:growth & development / Q000378:metabolism*; D024505:Tocopherols / Q000378:metabolism*; D024508:Tocotrienols / Q000378:metabolism*
chemical_list: D005227:Fatty Acids; D010938:Plant Oils; D017392:Thiobarbituric Acid Reactive Substances; D024508:Tocotrienols; D024505:Tocopherols