title: Toward potent antibiofilm degradable medical devices: a generic method for the antibacterial surface modification of polylactide.
abstract: The effects of biomaterials on their environment must be carefully modulated in most biomedical applications. Among other approaches, this modulation can be obtained through the modification of the biomaterial surface. This paper proposes a simple and versatile strategy to produce non-leaching antibacterial polylactide (PLA) surfaces without any degradation of the polyester chains. The method is based on a one-pot procedure that provides a "clickable" PLA surface via anionic activation which is then functionalized with an antibacterial quaternized poly(2-(dimethylamino)ethyl methacrylate) (QPDMAEMA) by covalent immobilization on the surface. The anti-adherence and antibiofilm activities of modified PLA surfaces are assessed for different QPDMAEMA molecular weights and different quaternization agents. Antibacterial PLA surfaces are shown to be very active against Gram-negative and Gram-positive strains, with adherence reduction factors superior to 99.999% and a marked reduction in biofilm on the most potent surfaces. In addition to this substantial antibacterial activity, the proposed PLA surfaces are also cytocompatible, as demonstrated through the proliferation of L929 fibroblasts.
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), Artificial Biopolymers Group, UMR CNRS 5247, University of Montpellier 1, University of Montpellier 2, Faculty of Pharmacy, 15 Av. C. Flahault, Montpellier 34093, France.
authors.forename: Sarah
authors.initials: S
authors.lastname: El Habnouni
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Lavigne
authors.forename: Vincent
authors.initials: V
authors.lastname: Darcos
authors.forename: Barbara
authors.initials: B
authors.lastname: Porsio
authors.forename: Xavier
authors.initials: X
authors.lastname: Garric
authors.forename: Jean
authors.initials: J
authors.lastname: Coudane
authors.forename: Benjamin
authors.initials: B
authors.lastname: Nottelet
mesh_terms: D020341:Absorbable Implants / Q000382:microbiology*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D018407:Bacterial Physiological Phenomena / Q000187:drug effects*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D003460:Crystallization / Q000379:methods; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D009757:Nylons / Q000737:chemistry / Q000494:pharmacology*; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry* / Q000494:pharmacology; D013499:Surface Properties
chemical_list: D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D008689:Methacrylates; D009757:Nylons; D011091:Polyesters; C407037:poly(2-(dimethylamino)ethyl methacrylate); C033616:poly(lactide)