title: Reconstruction of three dimensional structures from electron micrographs.
abstract: General principles are formulated for the objective reconstruction of a three dimensional object from a set of electron microscope images. These principles are applied to the calculation of a three dimensional density map of the tail of bacteriophage T4.
authors.affiliation: MRC Laboratory of Molecular Biology, Hills Road, Cambridge.
authors.forename: D J
authors.initials: DJ
authors.lastname: De Rosier
authors.forename: A
authors.initials: A
authors.lastname: Klug
mesh_terms: D017122:Bacteriophage T4 / Q000648:ultrastructure; D007091:Image Processing, Computer-Assisted; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008854:Microscopy, Electron / Q000379:methods*