title: Differential protein expression analysis of degenerative aortic stenosis by iTRAQ labeling.
abstract: Degenerative aortic stenosis is the most common worldwide cause of valve replacement. While it shares certain risk factors with coronary artery disease, it is not delayed or reversed by reducing exposure to risk factors (e.g., therapies that lower lipids). Therefore, it is necessary to better understand its pathophysiology for preventive measures to be taken. In order to identify proteins that are involved in AS, we designed a simple method for protein extraction, digestion, labeling, identification, and differential protein expression analysis of both normal and stenotic aortic valve to identify biomarkers of this pathology, which may facilitate early diagnosis and treatment.
authors.affiliation: Department of Vascular Physiopathology, Hospital Nacional de Paraplejicos, SESCAM, Toledo, Spain.
authors.forename: Sergio
authors.initials: S
authors.lastname: Alonso-Orgaz
authors.forename: Tatiana
authors.initials: T
authors.lastname: Martin-Rojas
authors.forename: Enrique
authors.initials: E
authors.lastname: Calvo
authors.forename: Juan Antonio
authors.initials: JA
authors.lastname: López
authors.forename: Fernando
authors.initials: F
authors.lastname: Vivanco
authors.forename: María G
authors.initials: MG
authors.lastname: Barderas
mesh_terms: D001021:Aortic Valve / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D001024:Aortic Valve Stenosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D010449:Peptide Mapping; D059748:Proteolysis; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D013194:Staining and Labeling / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D020543:Proteome