title: [Effect of Huangqi gegen decoction (HGD) on TGF-beta1/Smad3 pathway in diabetic cardiomyopathy rats].
abstract: OBJECTIVE
  To study the effect of HGD on diabetic cardiomyopathy and its mechanism.
  
  
  METHODS
  The T2-DM rats model was established by combining high fat diet with STZ. The blood glucose, insulin, myocardial fibrosis and TGF-beta1/Smad3 signaling pathway were observed; TGF-beta1 and Smad3 mRNA expression were detected by RT-PCR method, protein expression detected by immunohistochemical method.
  
  
  RESULTS
  HGD obviously reduced fasting blood glucose, insulin, improved insulin resistance, reduced myocardial hydroxyproline contents, lowered cardiac index, significantly inhibited over-expression of TGF-beta1/SMAD3 mRNA and protein in diabetic rats cardiac.
  
  
  CONCLUSIONS
  HGD can obviously prevent experimental diabetic myocardial fibrosis through the regulation effect on TGFbeta1/Smad3 signaling pathway.
authors.affiliation: School of Traditional Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China.
authors.forename: Yan-Fen
authors.initials: YF
authors.lastname: Chen
authors.forename: Chun-Yi
authors.initials: CY
authors.lastname: Wang
authors.forename: Wei-Min
authors.initials: WM
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Gao
authors.forename: Qing-He
authors.initials: QH
authors.lastname: Wu
authors.forename: Chun-Ping
authors.initials: CP
authors.lastname: Tang
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Chao-Yan
authors.initials: CY
authors.lastname: Yang
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000150:complications* / Q000378:metabolism; D058065:Diabetic Cardiomyopathies / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects; D051900:Smad3 Protein / Q000235:genetics / Q000378:metabolism*; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism*
chemical_list: D001786:Blood Glucose; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D051900:Smad3 Protein; D053773:Transforming Growth Factor beta1