title: Changes in tau phosphorylation in hibernating rodents.
abstract: Tau is a cytoskeletal protein present mainly in the neurons of vertebrates. By comparing the sequence of tau molecule among different vertebrates, it was found that the variability of the N-terminal sequence in tau protein is higher than that of the C-terminal region. The N-terminal region is involved mainly in the binding of tau to cellular membranes, whereas the C-terminal region of the tau molecule contains the microtubule-binding sites. We have compared the sequence of Syrian hamster tau with the sequences of other hibernating and nonhibernating rodents and investigated how differences in the N-terminal region of tau could affect the phosphorylation level and tau binding to cell membranes. We also describe a change, in tau phosphorylation, on a casein kinase 1 (ck1)-dependent site that is found only in hibernating rodents. This ck1 site seems to play an important role in the regulation of tau binding to membranes.
authors.affiliation: Laboratorio Cajal de Circuitos Corticales, Universidad Politécnica de Madrid, Campus Montegancedo, Pozuelo de Alarcón, Spain.
authors.forename: Gonzalo
authors.initials: G
authors.lastname: León-Espinosa
authors.forename: Esther
authors.initials: E
authors.lastname: García
authors.forename: Vega
authors.initials: V
authors.lastname: García-Escudero
authors.forename: Félix
authors.initials: F
authors.lastname: Hernández
authors.forename: Javier
authors.initials: J
authors.lastname: Defelipe
authors.forename: Jesús
authors.initials: J
authors.lastname: Avila
mesh_terms: D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D019556:COS Cells; D047389:Casein Kinase I / Q000378:metabolism; D002522:Chlorocebus aethiops; D006224:Cricetinae; D004791:Enzyme Inhibitors / Q000494:pharmacology; D019143:Evolution, Molecular; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism; D006801:Humans; D007093:Imidazoles / Q000494:pharmacology; D008094:Lithium / Q000494:pharmacology; D008297:Male; D008647:Mesocricetus; D010766:Phosphorylation / Q000187:drug effects / Q000502:physiology*; D011485:Protein Binding; D017422:Sequence Analysis, DNA; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism; D014162:Transfection; D016875:tau Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: C493177:4-(4-(2,3-dihydrobenzo(1,4)dioxin-6-yl)-5-pyridin-2-yl-1H-imidazol-2-yl)benzamide; D001549:Benzamides; D004791:Enzyme Inhibitors; D007093:Imidazoles; C054369:MAPT protein, human; D016875:tau Proteins; D008094:Lithium; D047389:Casein Kinase I; D038362:Glycogen Synthase Kinase 3