title: Effect of two synthetic disulfide bond variants of a 13-mer toxin from Conus californicus on the transcription of pro-inflammatory cytokines induced by LPS.
abstract: This study presents the effects of two synthetic disulfide bond variants of cal16b, a 13-mer Ca²⁺ channel blocker conotoxin, on pro- and anti-inflammatory cytokine gene transcription in the murine macrophage-like cell line J774A.1 stimulated with LPS. The globular form (cal16b_1) acted as an anti-inflammatory agent; in contrast, the ribbon disulfide variant (cal16b_2) had a pro-inflammatory effect. Our results suggest that the pro- and anti-inflammatory effects are mediated by the three-dimensional structure.
authors.affiliation: Marine Science Faculty, Universidad Autónoma de Baja California-UABC, Km 103, Carretera Tijuana-Ensenada, CP. 22800 Ensenada, B.C., México.
authors.forename: K E
authors.initials: KE
authors.lastname: Cervantes-Luevano
authors.forename: Johanna
authors.initials: J
authors.lastname: Bernaldez
authors.forename: Alexei
authors.initials: A
authors.lastname: Licea
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002460:Cell Line; D020916:Conotoxins / Q000494:pharmacology*; D052078:Conus Snail / Q000737:chemistry*; D016207:Cytokines / Q000378:metabolism*; D004220:Disulfides / Q000494:pharmacology*; D005786:Gene Expression Regulation; D007249:Inflammation / Q000378:metabolism; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D020916:Conotoxins; D016207:Cytokines; D004220:Disulfides; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha