title: Preparation of visible-light-excited europium biolabels for time-resolved luminescence cell imaging application.
abstract: By using a visible-light-excited ternary Eu(3+) complex, BHHBCB-Eu(3+)-BPT (BHHBCB: 1,2-bis[4'-(1",1",1",2",2",3",3"-heptafluoro-4″,6″-hexanedion-6″-yl)-benzyl]-4-chlorosulfobenzene; BPT: 2-(N,N-diethylanilin-4-yl)-4,6-bis(pyrazol-1-yl)-1,3,5-triazine), as a luminophore, two kinds of novel visible-light-excited europium materials, the silica-encapsulated BHHBCB-Eu(3+)-BPT (Eu@SiO2) nanoparticles and BHHBCB-Eu(3+)-BPT-conjugated bovine serum albumin (BSA-BHHBCB-Eu(3+)-BPT), were prepared for biolabeling and time-resolved luminescence cell imaging applications. The Eu@SiO2 nanoparticles, prepared by the copolymerization of 3-aminopropyl(triethoxy)silane-BHHBCB-Eu(3+)-BPT conjugate, free 3-aminopropyl(triethoxy) silane and tetraethyl orthosilicate in a water-in-oil reverse microemulsion, are monodispersed, spherical and uniform in size, and strongly luminescent with an excitation peak at ≈ 400 nm and a long luminescence lifetime of 346 μs. The BSA-BHHBCB-Eu(3+)-BPT, prepared by covalent binding of BHHBCB-Eu(3+)-BPT to BSA, shows also strong visible-light-excited luminescence with a excitation peak at ≈ 400 nm and a long luminescence lifetime of 402μs. The two materials were used for labeling transferrin and folic acid. Using the time-resolved luminescence imaging of living HeLa cells, the cell-surface receptors of transferrin and folic acid were successfully visualized by the prepared biolabels based on the ligand-receptor affinity binding interaction. The results demonstrated the feasibility of the new materials as visible-light-excited biolabels for the time-resolved luminescence cell imaging.
authors.affiliation: State Key Laboratory of Fine Chemicals, School of Chemistry, Dalian University of Technology, Dalian 116024, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.forename: Lu
authors.initials: L
authors.lastname: Tian
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Ye
authors.forename: Bo
authors.initials: B
authors.lastname: Song
authors.forename: Jingli
authors.initials: J
authors.lastname: Yuan
mesh_terms: D003952:Diagnostic Imaging; D005063:Europium / Q000737:chemistry* / Q000528:radiation effects; D005492:Folic Acid / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008027:Light; D049449:Luminescence; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry* / Q000528:radiation effects / Q000648:ultrastructure; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000528:radiation effects; D012822:Silicon Dioxide / Q000737:chemistry* / Q000528:radiation effects; D014168:Transferrin / Q000737:chemistry
chemical_list: D014168:Transferrin; D012710:Serum Albumin, Bovine; D005063:Europium; D012822:Silicon Dioxide; D005492:Folic Acid