title: [Inhibitory effect of human umbilical cord-derived mesenchymal stem cells on interleukin-17 production in peripheral blood T cells from spondyloarthritis patients].
abstract: In this study, the inhibitory effect of human umbilical cord-derived mesenchymal stem cells (hUCMSC) on interleukin-17 (IL-17) production in peripheral blood T cells from patients with spondyloarthritis (SpA) were investigated, in order to explore the therapeutic potential of hUCMSC in the SpA. Peripheral blood mononuclear cells (PBMNC) were isolated from patients with SpA (n = 12) and healthy subjects (n = 6). PBMNC were cultured in vitro with hUCMSC or alone. The expression of IL-17 in CD4(+) T cells or γ/δ T cells were determined in each subject group by flow cytometry. IL-17 concentrations in PBMNC culture supernatants were measured by ELISA. The results indicated that the proportion of IL-17-producing CD4(+) T cells and IL-17-producing γ/δ T cells of SpA patients were 4.5 folds and 5 folds of healthy controls [CD3(+)CD4(+)IL-17(+) cells (3.42 ± 0.82)% vs (0.75 ± 0.25)%, P < 0.01; CD3(+)γδTCR(+)IL-17(+) cells (0.30 ± 0.10)% vs (0.06 ± 0.02)%, P < 0.01]. After co-culture of PBMNC in patients with hUCMSC, the increased proportions of CD3(+)CD4(+)IL-17(+) cells and CD3(+)γδTCR(+)IL-17(+) cells in SpA patients were inhibited significantly by hUCMSC [CD3(+)CD4(+)IL-17(+) cells (3.42 ± 0.82)% vs (1.81 ± 0.59)% (P < 0.01); CD3(+)γδTCR(+)IL-17(+) cells (0.30 ± 0.10)% vs (0.16 ± 0.06)% (P < 0.01]. In response to phytohemagglutinin (PHA, 1 µg/ml), PBMNC from SpA patients secreted more IL-17 than that from healthy control [(573.95 ± 171.68) pg/ml vs (115.53 ± 40.41) pg/ml (P < 0.01)]. In the presence of hUCMSC, PBMNC of SpA patients produced less amount of IL-17 [(573.95 ± 171.68) pg/ml vs (443.20 ± 147.94) pg/ml, (P < 0.01)]. It is concluded that the IL-17 production in peripheral blood T cells from SpA patients can be inhibited by hUCMSC, which have therapeutic potential for SpA.
authors.affiliation: Department of Rheumatology, Chinese PLA General Hospital, Beijing, China.
authors.forename: Zhi-Fang
authors.initials: ZF
authors.lastname: Huang
authors.forename: Jian
authors.initials: J
authors.lastname: Zhu
authors.forename: Shuang-Hong
authors.initials: SH
authors.lastname: Lu
authors.forename: Jiang-Lin
authors.initials: JL
authors.lastname: Zhang
authors.forename: Xian-Da
authors.initials: XD
authors.lastname: Chen
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: DU
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Yang
authors.forename: Ya-Kun
authors.initials: YK
authors.lastname: Song
authors.forename: Dong-Ying
authors.initials: DY
authors.lastname: Wu
authors.forename: Bing
authors.initials: B
authors.lastname: Liu
authors.forename: Feng
authors.initials: F
authors.lastname: Huang
mesh_terms: D006801:Humans; D020381:Interleukin-17 / Q000378:metabolism*; D007963:Leukocytes, Mononuclear / Q000166:cytology; D018655:Lymphocyte Count; D059630:Mesenchymal Stem Cells; D025241:Spondylarthritis / Q000097:blood* / Q000378:metabolism / Q000628:therapy; D013601:T-Lymphocytes / Q000378:metabolism*; D014470:Umbilical Cord / Q000166:cytology
chemical_list: D020381:Interleukin-17