title: [Detection of guanylate cyclase C mRNA and cytokeratin 20 mRNA in peripheral blood and analysis of prognosis in early to moderate colorectal cancer patients].
abstract: OBJECTIVE
  To investigate the associations of guanylate cyclase C (GC-C) mRNA and cytokeratin 20 (CK20) mRNA with metastasis and prognosis in early to moderate colorectal cancer patients.
  
  
  METHODS
  GC-C mRNA and CK 20 mRNA in peripheral blood of 74 colorectal cancer patients without distant metastasis were detected by fluorescent quantitative PCR (FQ-PCR). Based on their clinicopathological and postoperative follow-up data, the relationship and clinical significance of these data with metastasis hazards and prognosis factors were analyzed.
  
  
  RESULTS
  The positive rate of GC-C mRNA in 74 colorectal cancer patients was 33.8% (25/74), and CK20 mRNA was 31.1% (23/74). The 1-, 2-, 3- year disease-free survival rates of patients were 94.6%, 82.4% and 78.4% respectively. There were significant differences in positive rates of GC-C mRNA and CK20 mRNA, tumor differentiation, mesentery lymph node metastasis, tumor embolus in vessel and postoperative chemotherapy associated with 3-year disease free survival rate by Kaplan-Meier analysis (all P<0.05). While mesentery lymph node metastasis and tumor embolus in vessel were independent risk factors of 3-year disease-free survival (P<0.05). CK20 mRNA and tumor embolus in vessel were independent risk factors of 3-year disease-free survival by analysis stratified with clinical stage (P<0.05).
  
  
  CONCLUSIONS
  Detection of CK20 mRNA and GC-C mRNA in peripheral blood may be important for early detection of early metastasis of colorectal cancer.
authors.affiliation: Department of Colorectal Cancer, Zhejiang Cancer Hospital, Hangzhou, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Jian-guo
authors.initials: JG
authors.lastname: Feng
authors.forename: Jun
authors.initials: J
authors.lastname: Qian
authors.forename: Hai-xing
authors.initials: HX
authors.lastname: Ju
authors.forename: Yin-bo
authors.initials: YB
authors.lastname: Chen
authors.forename: Hai-yang
authors.initials: HY
authors.lastname: Feng
authors.forename: Yu-ping
authors.initials: YP
authors.lastname: Zhu
authors.forename: De-chuan
authors.initials: DC
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015179:Colorectal Neoplasms / Q000097:blood*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D053554:Keratin-20 / Q000097:blood* / Q000235:genetics; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D012333:RNA, Messenger / Q000097:blood / Q000235:genetics; D017461:Receptors, Atrial Natriuretic Factor / Q000097:blood* / Q000235:genetics; D012307:Risk Factors
chemical_list: D053554:Keratin-20; D012333:RNA, Messenger; D017461:Receptors, Atrial Natriuretic Factor; C073616:atrial natriuretic factor receptor C