title: The identification of candidate radio marker genes using a coexpression network analysis in gamma-irradiated rice.
abstract: Plant physiological and biochemical processes are significantly affected by gamma irradiation stress. In addition, gamma-ray (GA) differentially affects gene expression across the whole genome. In this study, we identified radio marker genes (RMGs) responding only to GA stress compared with six abiotic stresses (chilling, cold, anoxia, heat, drought and salt) in rice. To analyze the expression patterns of differentially expressed genes (DEGs) in gamma-irradiated rice plants against six abiotic stresses, we conducted a hierarchical clustering analysis by using a complete linkage algorithm. The up- and downregulated DEGs were observed against six abiotic stresses in three and four clusters among a total of 31 clusters, respectively. The common gene ontology functions of upregulated DEGs in clusters 9 and 19 are associated with oxidative stress. In a Pearson's correlation coefficient analysis, GA stress showed highly negative correlation with salt stress. On the basis of specific data about the upregulated DEGs, we identified the 40 candidate RMGs that are induced by gamma irradiation. These candidate RMGs, except two genes, were more highly induced in rice roots than in other tissues. In addition, we obtained other 38 root-induced genes by using a coexpression network analysis of the specific upregulated candidate RMGs in an ARACNE algorithm. Among these genes, we selected 16 RMGs and 11 genes coexpressed with three RMGs to validate coexpression network results. RT-PCR assay confirmed that these genes were highly upregulated in GA treatment. All 76 genes (38 root-induced genes and 38 candidate RMGs) might be useful for the detection of GA sensitivity in rice roots.
authors.affiliation: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Jeonbuk, 580-185, Republic of Korea.
authors.forename: Sun-Hee
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Plant Genomics Lab, Department of Applied Plant Sciences, Kangwon National University, Chuncheon, 200-713, Republic of Korea.
authors.forename: Sun-Goo
authors.initials: SG
authors.lastname: Hwang
authors.affiliation: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Jeonbuk, 580-185, Republic of Korea.
authors.forename: Jung Eun
authors.initials: JE
authors.lastname: Hwang
authors.affiliation: Plant Genomics Lab, Department of Applied Plant Sciences, Kangwon National University, Chuncheon, 200-713, Republic of Korea.
authors.forename: Cheol Seong
authors.initials: CS
authors.lastname: Jang
authors.affiliation: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Jeonbuk, 580-185, Republic of Korea.
authors.forename: Vijayanand
authors.initials: V
authors.lastname: Velusamy
authors.affiliation: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Jeonbuk, 580-185, Republic of Korea.
authors.forename: Jin-Baek
authors.initials: JB
authors.lastname: Kim
authors.affiliation: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Jeonbuk, 580-185, Republic of Korea.
authors.forename: Sang Hoon
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Jeonbuk, 580-185, Republic of Korea.
authors.forename: Bo-Keun
authors.initials: BK
authors.lastname: Ha
authors.affiliation: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Jeonbuk, 580-185, Republic of Korea.
authors.forename: Si-Yong
authors.initials: SY
authors.lastname: Kang
authors.affiliation: Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Jeonbuk, 580-185, Republic of Korea.
authors.forename: Dong Sub
authors.initials: DS
authors.lastname: Kim
mesh_terms: D000222:Adaptation, Physiological; D015536:Down-Regulation / Q000528:radiation effects; D055864:Droughts; D005720:Gamma Rays; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects*; D063990:Gene Ontology; D005819:Genetic Markers / Q000528:radiation effects; D012275:Oryza / Q000235:genetics* / Q000528:radiation effects; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000528:radiation effects; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological; D015854:Up-Regulation / Q000528:radiation effects
chemical_list: D005819:Genetic Markers; D010940:Plant Proteins; D012965:Sodium Chloride