title: Preparation, chromatographic evaluation and comparison between linear peptide- and cyclopeptide-bonded stationary phases.
abstract: In this paper, linear and cyclic tetrapeptide bonded stationary phases, were synthesized by the solid-phase synthesis method. Each amino acid coupling step was monitored by the ninhydrin test using UV/visible spectrophotometry. The proposed materials were characterized by UV spectroscopy, Fourier transform infrared spectroscopy, elemental analysis and thermogravimetric analysis, which proved the successful immobilization of tetrapeptide on the silica support. The cyclopeptide stationary phase displayed mixed-mode behavior and had the size selectivity for polycyclic aromatic hydrocarbon such as anthracene, while the linear peptide stationary phase only exhibited typical hydrophilic interaction chromatography (HILIC) characteristics and was more hydrophilic under the same mobile phase condition. Retention behaviors of polar compounds on the two stationary phases were studied through varying column temperature, the water content, pH and ionic strength in mobile phase. Applications in the separation of the mixture of nucleosides, sulfa compounds, organic acids and isomers of substituted benzoic acid compounds were demonstrated.
authors.affiliation: Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Lanzhou University, Lanzhou 730000, China.
authors.forename: Jiao
authors.initials: J
authors.lastname: Li
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Li
authors.forename: Tong
authors.initials: T
authors.lastname: Chen
authors.forename: Luan
authors.initials: L
authors.lastname: Xu
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Liu
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhang
authors.forename: Haixia
authors.initials: H
authors.lastname: Zhang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D056148:Chromatography, Reverse-Phase / Q000295:instrumentation / Q000379:methods*; D057927:Hydrophobic and Hydrophilic Interactions; D009842:Oligopeptides / Q000737:chemistry*; D010456:Peptides, Cyclic / Q000737:chemistry*; D011487:Protein Conformation; D012822:Silicon Dioxide / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared / Q000295:instrumentation / Q000379:methods; D013818:Thermogravimetry / Q000295:instrumentation / Q000379:methods
chemical_list: D009842:Oligopeptides; D010456:Peptides, Cyclic; D012822:Silicon Dioxide