title: Improving the default data analysis workflow for large autoimmune biomarker discovery studies with ProtoArrays.
abstract: Contemporary protein microarrays such as the ProtoArray® are used for autoimmune antibody screening studies to discover biomarker panels. For ProtoArray data analysis, the software Prospector and a default workflow are suggested by the manufacturer. While analyzing a large data set of a discovery study for diagnostic biomarkers of the Parkinson's disease (ParkCHIP), we have revealed the need for distinct improvements of the suggested workflow concerning raw data acquisition, normalization and preselection method availability, batch effects, feature selection, and feature validation. In this work, appropriate improvements of the default workflow are proposed. It is shown that completely automatic data acquisition as a batch, a re-implementation of Prospector's pre-selection method, multivariate or hybrid feature selection, and validation of the selected protein panel using an independent test set define in combination an improved workflow for large studies.
authors.affiliation: Medizinisches Proteom-Center, Ruhr-University Bochum, Bochum, Germany. michael.turewicz@rub.de
authors.forename: Michael
authors.initials: M
authors.lastname: Turewicz
authors.forename: Caroline
authors.initials: C
authors.lastname: May
authors.forename: Maike
authors.initials: M
authors.lastname: Ahrens
authors.forename: Dirk
authors.initials: D
authors.lastname: Woitalla
authors.forename: Ralf
authors.initials: R
authors.lastname: Gold
authors.forename: Swaantje
authors.initials: S
authors.lastname: Casjens
authors.forename: Beate
authors.initials: B
authors.lastname: Pesch
authors.forename: Thomas
authors.initials: T
authors.lastname: Brüning
authors.forename: Helmut E
authors.initials: HE
authors.lastname: Meyer
authors.forename: Eckhard
authors.initials: E
authors.lastname: Nordhoff
authors.forename: Miriam
authors.initials: M
authors.lastname: Böckmann
authors.forename: Christian
authors.initials: C
authors.lastname: Stephan
authors.forename: Martin
authors.initials: M
authors.lastname: Eisenacher
mesh_terms: D000544:Alzheimer Disease / Q000276:immunology; D001323:Autoantibodies / Q000032:analysis*; D015415:Biomarkers / Q000032:analysis; D019295:Computational Biology / Q000379:methods*; D030562:Databases, Protein; D006801:Humans; D010300:Parkinson Disease / Q000276:immunology; D040081:Protein Array Analysis / Q000379:methods*; D015203:Reproducibility of Results; D012984:Software
chemical_list: D001323:Autoantibodies; D015415:Biomarkers
keywords: Autoantibodies; Bioinformatics; Biological markers; Parkinson's disease; Protein array analysis