title: [The effect of PNS on the content and activity of alpha-secretase in the brains of SAMP8 mice with alzheimer's disease].
abstract: OBJECTIVE
  To explore the effects of PNS on the content and activity of alpha-secretase in the brains of SAMP8 mice with Alzheimer's disease.
  
  
  METHODS
  SAMP8 mice were randomly divided into four groups: PNS high-dosage group, PNS low-dosage group, huperzine A group and control group. The high-dosage group and low-dosage group were treated with 200 and 100 mg/kg PNS respectively per day and the huperzine A group was treated with 0.3 mg/kg huperzine A per day, all by intragastric administration for 8 consecutive weeks. The same volume of double distilled water was given to the control group. The activity of a-secretase was assayed by direct immunofluorescent method(DIF). Western blot was used to detect the content of alpha-secretase including ADAM9, ADAM10 and ADAM17 proteins.
  
  
  RESULTS
  The Relative Fluorescence Units (RFU) of PNS high-dosage and low-dosage groups were higher than that of control group (P < 0.01). The results of western blot showed that the level of ADAM9 protein expression in PNS high-dosage, low-dosage and huperzine A groups was significantly higher than that of control group (P < 0.05) while the levels of ADAM10 protein expression in PNS high-dosage, low-dosage and huperzine A groups was significantly lower than that of control group (P < 0.05), while level of ADAM17 of huperzine A group was higher than that of control group (P < 0.05).
  
  
  CONCLUSIONS
  PNS can increase activity of alpha-secretase in the brain of SAMP8 mouse via increasing the level of ADAM9 protein expression.
authors.affiliation: Guangxi Medical University, Nanning 530021,China. hj0221104@163.com
authors.forename: Jin-Lan
authors.initials: JL
authors.lastname: Huang
authors.forename: Deng-Pan
authors.initials: DP
authors.lastname: Wu
authors.forename: Lu
authors.initials: L
authors.lastname: Lu
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.forename: Zhen-Guo
authors.initials: ZG
authors.lastname: Zhong
mesh_terms: D051722:ADAM Proteins / Q000378:metabolism; D000072197:ADAM10 Protein; D000072198:ADAM17 Protein; D000375:Aging / Q000378:metabolism; D000544:Alzheimer Disease / Q000188:drug therapy* / Q000378:metabolism; D053829:Amyloid Precursor Protein Secretases / Q000378:metabolism*; D016564:Amyloid beta-Protein Precursor / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D053723:Panax notoginseng; D011897:Random Allocation; D012503:Saponins / Q000494:pharmacology*
chemical_list: D016564:Amyloid beta-Protein Precursor; D008565:Membrane Proteins; D012503:Saponins; D053829:Amyloid Precursor Protein Secretases; D051722:ADAM Proteins; C498316:Adam9 protein, mouse; D000072197:ADAM10 Protein; C491234:Adam10 protein, mouse; D000072198:ADAM17 Protein; C000606463:Adam17 protein, mouse