title: Preparation of molecularly imprinted polymers on the surface of magnetic carbon nanotubes with a pseudo template for rapid simultaneous extraction of four fluoroquinolones in egg samples.
abstract: Fluoroquinolones (FQs) have emerged as one of the most important class of antibiotics. Due to their low concentration in bio-matrix samples which contain a lot of interfering substances, the efficient solid phase extraction and accurate determination of FQs remain a challenge. In this paper, a new strategy for the isolation and enrichment of FQs from egg samples was obtained by molecularly imprinted polymers on the surface of magnetic carbon nanotubes (MCNTs@MIP), which not only can be collected and separated rapidly by an external magnetic field, but also have a high specific surface area, outstanding mechanical properties and specific recognition for FQs. MCNTs@MIP were prepared using ofloxacin as a pseudo template, methacrylic acid as a functional monomer, and ethylene glycol dimethacrylate as a cross-linker. The characteristics of the MCNTs@MIP were assessed by transmission electron microscopy (TEM), multipoint Brunauer-Emmett-Teller (BET) analysis, vibrating sample magnetometry (VSM), X-ray diffraction (XRD) and Fourier transform infrared (FT-IR) spectroscopy. The results of the adsorption experiments not only demonstrated rapid dynamic adsorption but also showed a high selectivity toward FQs. An extraction method using MCNTs@MIP coupled with high performance liquid chromatography (HPLC) was developed for the determination of four FQs in egg samples. The recovery of four FQs ranged from 95.2% ± 3.2% to 100.7% ± 3.1% and the detection limits ranged from 0.25-0.40 ng g(-1). The results demonstrate that the proposed method based on pseudo template MCNTs@MIP is a promising approach for the preconcentration, purification, and simultaneous analysis of four FQs in bio-matrix samples.
authors.affiliation: Department of Analytical Chemistry, China Pharmaceutical University, 24 Tongjia Lane, Nanjing 210009, Jiangsu, China.
authors.forename: Deli
authors.initials: D
authors.lastname: Xiao
authors.forename: Pierre
authors.initials: P
authors.lastname: Dramou
authors.forename: Nanqian
authors.initials: N
authors.lastname: Xiong
authors.forename: Hua
authors.initials: H
authors.lastname: He
authors.forename: Danhua
authors.initials: D
authors.lastname: Yuan
authors.forename: Hao
authors.initials: H
authors.lastname: Dai
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.forename: Xiaomei
authors.initials: X
authors.lastname: He
authors.forename: Jun
authors.initials: J
authors.lastname: Peng
authors.forename: Nan
authors.initials: N
authors.lastname: Li
mesh_terms: D000327:Adsorption; D002851:Chromatography, High Pressure Liquid; D024841:Fluoroquinolones / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification*; D059346:Magnets / Q000737:chemistry; D054802:Molecular Imprinting; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010063:Ovum / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis*; D013499:Surface Properties; D013997:Time Factors
chemical_list: D024841:Fluoroquinolones; D037742:Nanotubes, Carbon; D011108:Polymers