title: Identification of sequence-related amplified polymorphism markers linked to the red leaf trait in ornamental kale (Brassica oleracea L. var. acephala).
abstract: Artistic diversiform leaf color is an important agronomic trait that affects the market value of ornamental kale. In the present study, genetic analysis showed that a single-dominant gene, Re (red leaf), determines the red leaf trait in ornamental kale. An F2 population consisting of 500 individuals from the cross of a red leaf double-haploid line 'D05' with a white leaf double-haploid line 'D10' was analyzed for the red leaf trait. By combining bulked segregant analysis and sequence-related amplified polymorphism technology, we identified 3 markers linked to the Re/re locus. A genetic map of the Re locus was constructed using these sequence-related amplified polymorphism markers. Two of the markers, Me8Em4 and Me8Em17, were located on one side of Re/re at distances of 2.2 and 6.4 cM, whereas the other marker, Me9Em11, was located on the other side of Re/re at a distance of 3.7 cM. These markers could be helpful for the subsequent cloning of the red trait gene and marker-assisted selection in ornamental kale breeding programs.
authors.affiliation: Department of Horticulture, Shenyang Agricultural University, Shenyang, China.
authors.forename: Y S
authors.initials: YS
authors.lastname: Wang
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Liu
authors.forename: Y F
authors.initials: YF
authors.lastname: Li
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.forename: X F
authors.initials: XF
authors.lastname: Yang
authors.forename: H
authors.initials: H
authors.lastname: Feng
mesh_terms: D001937:Brassica / Q000235:genetics*; D017343:Genes, Plant; D008040:Genetic Linkage; D005819:Genetic Markers; D010641:Phenotype; D010860:Pigments, Biological / Q000235:genetics*; D018515:Plant Leaves / Q000235:genetics*; D011110:Polymorphism, Genetic; D040641:Quantitative Trait Loci; D019655:Quantitative Trait, Heritable; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D005819:Genetic Markers; D010860:Pigments, Biological