title: RAPD and phytochemical analysis of Thymus moroderi plantlets after cryopreservation.
abstract: Cryopreservation is at present the most reliable strategy to preserve plant germplasm. When aromatic plants are the object of conservation it is necessary to assess not only the genetic but also the phytochemical stability to ensure that plant material maintains its qualities after storage. In this work we present molecular and phytochemical stability data related to a previously described vitrification-based cryopreservation protocol for Thymus moroderi Pau ex Martínez. RAPD markers have been used to assess the genetic stability of T. moroderi explants and revealed 0.34 percent of variation in the cryopreserved material studied. Phytochemical data collected from GC-MS analysis of dichloromethane extracts from cryopreserved plantlets rendered a profile in which 1,8-cineole (14.5 percent), camphor (5.9 percent) and borneol (5.2 percent) were the major components. Both data confirmed the suitability of the cryopreservation protocol applied.
authors.affiliation: Laboratorio de Biotecnologia Vegetal, Instituto Universitario de Investigacion CIBIO (Centro Iberoamericano de la Biodiversidad), Universidad de Alicante, Crta. San Vicente del Raspeig s/n. E-03690 San Vicente del Raspeig, Alicante, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Marco-Medina
authors.forename: José Luis
authors.initials: JL
authors.lastname: Casas
mesh_terms: D000064:Acclimatization; D001892:Camphanes / Q000032:analysis; D002164:Camphor / Q000032:analysis; D015925:Cryopreservation / Q000379:methods; D003511:Cyclohexanols / Q000032:analysis; D018744:DNA, Plant / Q000235:genetics; D000077591:Eucalyptol; D039821:Monoterpenes / Q000032:analysis; D019105:Random Amplified Polymorphic DNA Technique; D046930:Thymus Plant / Q000737:chemistry* / Q000235:genetics* / Q000502:physiology; D058989:Vitrification; D055549:Volatile Organic Compounds / Q000032:analysis
chemical_list: D001892:Camphanes; D003511:Cyclohexanols; D018744:DNA, Plant; D039821:Monoterpenes; D055549:Volatile Organic Compounds; D002164:Camphor; C022871:isoborneol; D000077591:Eucalyptol