title: Roughened titanium surfaces with silane and further RGD peptide modification in vitro.
abstract: The strategy to achieve osteoregeneration of dental implants during early-stage regeneration is strongly related to surface conditions for achieving highly successful effects after implantation. Surface modifications, namely, mechanical ground, silanization, bonded and sandblasted with pentasequence Gly-Arg-Gly-Asp-Ser (GRGDS) peptide, and acid-etched with Arg-Gly-Asp (RGD) peptide, were compared for their ability to support cell attachment, proliferation, and differentiation on titanium surfaces. The characteristics and comparative in vitro bio-interactions toward osteoprogenitor cells were tested in the four groups with various surface modifications. Compared with the other groups, the sandblasted and acid-etched, and silane with subsequent RGD peptide modified surfaces had the smallest wetting angle, absence of a significant cell viability difference, and largest quantity of alkaline phosphatase production during the expressions of early-stage cell differentiation. The method of synthesizing GRGDS peptides on roughened titanium surfaces has the potential to provide a combination of early bone regeneration and implant of long-term anchored capabilities.
authors.affiliation: Advanced Medical Devices and Composites Laboratory, Department of Fiber and Composite Materials, College of Engineering, Feng Chia University, Taichung 40724, Taiwan, ROC. wencchen@fcu.edu.tw
authors.forename: Wen-Cheng
authors.initials: WC
authors.lastname: Chen
authors.forename: Chia-Ling
authors.initials: CL
authors.lastname: Ko
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D015921:Dental Implants; D051379:Mice; D008807:Mice, Inbred BALB C; D009842:Oligopeptides / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology; D012821:Silanes / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D017155:Wettability
chemical_list: D015921:Dental Implants; D009842:Oligopeptides; D012821:Silanes; C047981:arginyl-glycyl-aspartic acid; D014025:Titanium