title: Label-free microRNA detection based on exchange-induced remnant magnetization.
abstract: We report a technique that is based on exchange-induced remnant magnetization for microRNA (miRNA) detection. In sequence-specific exchange reactions between label-free miRNA and magnetically labelled RNA with one mismatched base, the decrease in magnetization quantitatively represents the target miRNA. The detection limit reaches the zeptomole regime, with no amplification or washing procedures. Therefore, our technique will be suitable for precise miRNA profiling to aid in early diagnosis of cancers.
authors.affiliation: Department of Chemistry, University of Houston, Houston, Texas 77204, USA.
authors.forename: Li
authors.initials: L
authors.lastname: Yao
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Wang
authors.forename: Shoujun
authors.initials: S
authors.lastname: Xu
mesh_terms: D020137:Base Pair Mismatch; D001483:Base Sequence; D004247:DNA / Q000032:analysis / Q000235:genetics; D057230:Limit of Detection; D008280:Magnetics / Q000379:methods*; D059346:Magnets / Q000737:chemistry*; D035683:MicroRNAs / Q000032:analysis* / Q000235:genetics; D012680:Sensitivity and Specificity; D017423:Sequence Analysis, RNA / Q000379:methods*
chemical_list: D035683:MicroRNAs; D004247:DNA