title: Bioautography-guided isolation of antibacterial compounds of essential oils from Thai spices against histamine-producing bacteria.
abstract: The outbreak of histamine fish poisoning has been being an issue in food safety and international trade. The growth of contaminated bacterial species including Morganella morganii which produce histidine decarboxylase causes histamine formation in fish during storage. Histamine, the main toxin, causes mild to severe allergic reaction. At present, there is no well-established solution for histamine fish poisoning. This study was performed to determine the antibacterial activity of essential oils from Thai spices against histamine-producing bacteria. Among the essential oils tested, clove, lemongrass and sweet basil oils were found to possess the antibacterial activity. Clove oil showed the strongest inhibitory activity against Morganella morganii, followed by lemongrass and sweet basil oils. The results indicated that clove, lemongrass and sweet basil oils could be useful for the control of histamine-producing bacteria. The attempt to identify the active components using preparative TLC and GC/MS found eugenol, citral and methyl chavicol as the active components of clove, lemongrass and sweet basil oils, respectively. The information from this study would be useful in the research and development for the control of histamine-producing bacteria in fish or seafood products to reduce the incidence of histamine fish poisoning.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
authors.forename: Pattamapan
authors.initials: P
authors.lastname: Lomarat
authors.forename: Phanida
authors.initials: P
authors.lastname: Phanthong
authors.forename: Karn
authors.initials: K
authors.lastname: Wongsariya
authors.forename: Mullika Traidej
authors.initials: MT
authors.lastname: Chomnawang
authors.forename: Nuntavan
authors.initials: N
authors.lastname: Bunyapraphatsara
mesh_terms: D032429:Alpinia / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D031743:Cymbopogon / Q000737:chemistry; D006632:Histamine / Q000096:biosynthesis*; D006640:Histidine Decarboxylase / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D020613:Morganella morganii / Q000187:drug effects* / Q000378:metabolism; D018649:Ocimum basilicum / Q000737:chemistry; D009822:Oils, Volatile / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D017365:Spices; D027842:Syzygium / Q000737:chemistry; D030521:Zingiberaceae / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D009822:Oils, Volatile; D006632:Histamine; D006640:Histidine Decarboxylase