title: Ethyl acetate extract of the Mauritian sponge Jaspis sp. induces cell arrest in human promyelocytic leukemia cells.
abstract: Marine sponges are considered as a gold mine of new natural products possessing numerous biological activities. We examined the cytotoxic properties of the ethyl acetate extract (JDE) of the previously unrecorded sponge, Jaspis sp. collected from Mauritius Waters. JDE displayed an interesting IC50 of 0.057±0.04μg/mL on HL-60 cells evaluated by MTS assay. Mitochondrial membrane potential change, microscopic analysis and DNA fragmentation assays also confirmed JDE induced apoptosis on HL-60 cells. Annexin V staining demonstrated that JDE induced apoptosis at different concentrations. Treatment with 100ng/mL of JDE led to an accumulation of cells in G2/M phase after 24 h, causing a significant increase of cells (24h: 5.84%; 48h: 13.41%) in sub-G1 phase suggesting that JDE can induce cell cycle arrest in G2/M phase.
authors.affiliation: Mauritius Oceanography Institute, Quatre-Bornes, Mauritius.
authors.forename: Girish
authors.initials: G
authors.lastname: Beedessee
authors.forename: Avin
authors.initials: A
authors.lastname: Ramanjooloo
authors.forename: Geneviève
authors.initials: G
authors.lastname: Aubert
authors.forename: Laure
authors.initials: L
authors.lastname: Eloy
authors.forename: Deepak
authors.initials: D
authors.lastname: Arya
authors.forename: Rob W M
authors.initials: RW
authors.lastname: van Soest
authors.forename: Thierry
authors.initials: T
authors.lastname: Cresteil
authors.forename: Daniel E P
authors.initials: DE
authors.lastname: Marie
mesh_terms: D000085:Acetates / Q000737:chemistry; D000818:Animals; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D045424:Complex Mixtures / Q000494:pharmacology*; D003603:Cytotoxins / Q000494:pharmacology*; D053938:DNA Fragmentation; D018922:HL-60 Cells; D006801:Humans; D015473:Leukemia, Promyelocytic, Acute; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D011161:Porifera; D012997:Solvents / Q000737:chemistry
chemical_list: D000085:Acetates; D045424:Complex Mixtures; D003603:Cytotoxins; D012997:Solvents; C007650:ethyl acetate