title: [Construction and characterization of ompH gene knockout mutant of avian Pasteurella multocida C48-3].
abstract: OBJECTIVE
  To study the role of the outer membrane protein H (OmpH) in pathogenicity of avian Pasteurella multocida.
  
  
  METHODS
  The ompH knock-out mutant of avian P. multocida C48-3 was constructed by homologous recombination. The DNA replacement was confirmed by PCR, RT-PCR and Western blot. We compared the differences of biological characteristics such as growth rate, capsular structure, adhesion ability and virulence between the ompH knockout mutant of C48-3 Delta ompH and parent strain C48-3, as well as the complemented strain C48-3C.
  
  
  RESULTS
  C48-3 Delta ompH was successfully constructed. Electron microscopy examination of C48-3 Delta ompH shows the absence of capsular material compared to the parent strain C48-3 and complemented strain C48-3C. The adhesion assay shows that the number of C48-3 Delta ompH adhered to CEF cells was significantly lower than that of C48-3 and C48-3C. C48-3 Delta ompH was relatively attenuated in mice by intraperitoneal injection.
  
  
  CONCLUSIONS
  The construction of C48-3 Delta ompH would facilitate further study on pathogenesis of avian Pasteurella multocida.
authors.affiliation: College of Biology and Environmental Sciences, Jishou University, Jishou 416000, China. ulum@jsu.edu.cn
authors.forename: Wulumuhan
authors.initials: W
authors.lastname: Nazierbieke
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Zhang
authors.forename: Fengjuan
authors.initials: F
authors.lastname: Gong
authors.forename: Sawada
authors.initials: S
authors.lastname: Takuo
authors.forename: Entomack
authors.initials: E
authors.lastname: Borrathybay
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D002642:Chick Embryo; D005260:Female; D055786:Gene Knockout Techniques; D051379:Mice; D008807:Mice, Inbred BALB C; D016979:Pasteurella multocida / Q000235:genetics* / Q000472:pathogenicity* / Q000502:physiology; D011201:Poultry Diseases / Q000382:microbiology*; D014774:Virulence
chemical_list: D001425:Bacterial Outer Membrane Proteins; C067128:ompH protein, bacteria