title: High levels of polymorphism found through cross-amplification of microsatellite loci in a Ctenomys pearsoni (Rodentia, Ctenomyidae) population.
abstract: Ctenomys pearsoni (Pearson's tuco-tuco) is a subterranean rodent native to Uruguay. We tested the amplification pattern of 12 microsatellite loci, designed for C. sociabilis and C. haigi in a C. pearsoni population. DNA extractions were made from hair samples, and PCR amplification products were run on an ABI 3100 microcapillary gel. Eight loci were selected to form a highly polymorphic panel that could be used to efficiently screen populations of this species. In DNA from 35 tuco-tucos, the mean polymorphic information content value was 0.6536 and the mean expected heterozygosity was 0.7166. Paternity non-exclusion probabilities for seven independent loci were NE-1P = 0.0766 and NE-2P = 0.0108, and combined non-exclusion P(ID) was 6.2 x 10(-7). This panel of microsatellite loci has sufficient power to make inferences regarding group structure, mating strategies and evolutionary relationships among populations.
authors.affiliation: Genética de la Conservación, Departamento de Genética, Instituto de Investigaciones Biológicas Clemente Estable, Unidad Asociada Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay.
authors.forename: N
authors.initials: N
authors.lastname: Mannise
authors.forename: S
authors.initials: S
authors.lastname: González
authors.forename: J E
authors.initials: JE
authors.lastname: Maldonado
authors.forename: G
authors.initials: G
authors.lastname: Izquierdo
authors.forename: G
authors.initials: G
authors.lastname: Francescoli
authors.forename: M
authors.initials: M
authors.lastname: Cosse
mesh_terms: D000483:Alleles; D000818:Animals; D056426:Genetic Loci; D005828:Genetics, Population; D018895:Microsatellite Repeats; D021141:Nucleic Acid Amplification Techniques; D011110:Polymorphism, Genetic; D012377:Rodentia / Q000235:genetics*