title: [Determination of deoxynivalenol and deepoxy-deoxynivalenol in milk].
abstract: A HPLC method with UV/diode array detection for the determination of deoxynivalenol (DON) and deepoxy-deoxynivalenol (DOM-1) in milk was developed. Milk was incubated with β-glucuronidase and then defatted. After purification by immunoaffinity chromatography, DON and DOM-1 were separated on a C18 reversed phase column with acetonitril/water (10/90) as the mobile phase and detected at 218 nm. Limits of quantification were 1 μg/l for both toxins, with mean recoveries (1-10 μg/l) of 97% (DON) and 84% (DOM-1), respectively. Milk samples (pasteurized, UHT; n=32) from German retail shops were analysed by this method. Neither DON/DOM-1 nor their glucuronides were found in any sample. These results are consistent with published studies indicating that in lactating cows, DON and DOM-1 are mostly eliminated through urine, and that the carry-over into milk is negligible.
authors.affiliation: Institut für Tierärztliche Nahrungsmittelkunde, Justus-Liebig-Universität, Ludwigstr. 21, D-35390, Giessen, Milchwissenschaften@vetmed on-gessen.ce.
authors.forename: V
authors.initials: V
authors.lastname: Curtui
authors.forename: C
authors.initials: C
authors.lastname: Seidler
authors.forename: E
authors.initials: E
authors.lastname: Schneider
authors.forename: E
authors.initials: E
authors.lastname: Usleber