title: Myosin-Va-dependent cell-to-cell transfer of RNA from Schwann cells to axons.
abstract: To better understand the role of protein synthesis in axons, we have identified the source of a portion of axonal RNA. We show that proximal segments of transected sciatic nerves accumulate newly-synthesized RNA in axons. This RNA is synthesized in Schwann cells because the RNA was labeled in the complete absence of neuronal cell bodies both in vitro and in vivo. We also demonstrate that the transfer is prevented by disruption of actin and that it fails to occur in the absence of myosin-Va. Our results demonstrate cell-to-cell transfer of RNA and identify part of the mechanism required for transfer. The induction of cell-to-cell RNA transfer by injury suggests that interventions following injury or degeneration, particularly gene therapy, may be accomplished by applying them to nearby glial cells (or implanted stem cells) at the site of injury to promote regeneration.
authors.affiliation: Department of Proteins and Nucleic Acids, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay. sotelo@iibce.edu.uy
authors.forename: José R
authors.initials: JR
authors.lastname: Sotelo
authors.forename: Lucía
authors.initials: L
authors.lastname: Canclini
authors.forename: Alejandra
authors.initials: A
authors.lastname: Kun
authors.forename: José R
authors.initials: JR
authors.lastname: Sotelo-Silveira
authors.forename: Lei
authors.initials: L
authors.lastname: Xu
authors.forename: Horst
authors.initials: H
authors.lastname: Wallrabe
authors.forename: Aldo
authors.initials: A
authors.lastname: Calliari
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Rosso
authors.forename: Karina
authors.initials: K
authors.lastname: Cal
authors.forename: John A
authors.initials: JA
authors.lastname: Mercer
mesh_terms: D000199:Actins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001369:Axons / Q000378:metabolism*; D001692:Biological Transport; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D002450:Cell Communication; D015870:Gene Expression; D018995:Myosin Heavy Chains / Q000235:genetics / Q000378:metabolism*; D024701:Myosin Type V / Q000235:genetics / Q000378:metabolism*; D012313:RNA / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D012583:Schwann Cells / Q000166:cytology / Q000378:metabolism*; D012584:Sciatic Nerve / Q000166:cytology / Q000293:injuries / Q000378:metabolism*; D053778:Thiazolidines / Q000494:pharmacology
chemical_list: D000199:Actins; D019086:Bridged Bicyclo Compounds, Heterocyclic; C513081:Myo5a protein, rat; D053778:Thiazolidines; D012313:RNA; D024701:Myosin Type V; D018995:Myosin Heavy Chains; C037067:latrunculin A