title: Purification, characterization and antiviral activity of two heteropolysaccharides from Duchesneae Indicae.
abstract: This study was designed to uncover the antiviral compounds contained in Duchesneae Indicae. Two non-sulfated acidic heteropolysaccharides (DIP30 and DIP60) with anti-varicella-zoster virus (anti-VZV) activity were purified and investigated with a combination of DEAE C-52 ion-exchange chromatography, Sphacryl S-300 gel chromatography, high performance size exclusion chromatography, pre-column derivation with 1-phenyl-3-methyl-5-pyrazolone (PMP) method, infrared (IR) spectroscopy, (1)H and (13)C nuclear magnetic resonance (NMR). Results indicated that molecular weight (MW) of DIP30 and DIP60 are 1.98×10(3) kDa and 34 kDa, respectively. Both DIP30 and DIP60 have a backbone of β-conformation and consist of more than four kinds of monosaccharides. The anti-VZV 50% effective concentrations (EC50) of DIP30 and DIP60 are 265.2±35.4 μg/ml and 325.6±42 μg/ml, respectively, suggesting that DIP30 and DIP60 could be explored as novel antiviral agents.
authors.affiliation: School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, PR China.
authors.forename: Haifei
authors.initials: H
authors.lastname: Jiang
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.forename: An
authors.initials: A
authors.lastname: Zhou
authors.forename: Guangrong
authors.initials: G
authors.lastname: Xie
authors.forename: Luying
authors.initials: L
authors.lastname: Yan
authors.forename: Yutiao
authors.initials: Y
authors.lastname: Jiang
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Ye
authors.initials: Y
authors.lastname: Shen
authors.forename: Jianhua
authors.initials: J
authors.lastname: Chen
mesh_terms: D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002850:Chromatography, Gel; D004353:Drug Evaluation, Preclinical; D014645:Herpesvirus 3, Human / Q000187:drug effects*; D006801:Humans; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D031987:Potentilla / Q000737:chemistry*; D052616:Solid Phase Extraction; D017550:Spectroscopy, Fourier Transform Infrared; D013431:Sulfates / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000998:Antiviral Agents; D010936:Plant Extracts; D011134:Polysaccharides; D013431:Sulfates