title: A non-targeted approach for extended liquid chromatography-mass spectrometry profiling of free and esterified jasmonates after wounding.
abstract: Upon wounding or herbivory, plants quickly react by activating various defense mechanisms. A major part of these defenses is thought to be regulated by the jasmonate pathway through the induction of jasmonic acid and its biologically active jasmonoyl-isoleucine conjugate. Yet, these well-known phytohormones are only two among the numerous compounds that compose the jasmonate family. Here, we describe a method based on ultrahigh-pressure liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry that can potentially profile the full range of known free and esterified jasmonates in a non-targeted manner. The developed approach is illustrated by the analysis of Arabidopsis thaliana leaves after mechanical wounding.
authors.affiliation: Chemical Analytical Service of the Swiss Plant Science Web, Institute of Biology, University of Neuchâtel, Neuchâtel, Switzerland.
authors.forename: Gaëtan
authors.initials: G
authors.lastname: Glauser
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Wolfender
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods / Q000592:standards; D003517:Cyclopentanes / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods / Q000592:standards; D054883:Oxylipins / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D010937:Plant Growth Regulators / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism*; D012015:Reference Standards; D052616:Solid Phase Extraction
chemical_list: D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; C011006:jasmonic acid