title: Renal intercalated cells are rather energized by a proton than a sodium pump.
abstract: The Na(+) concentration of the intracellular milieu is very low compared with the extracellular medium. Transport of Na(+) along this gradient is used to fuel secondary transport of many solutes, and thus plays a major role for most cell functions including the control of cell volume and resting membrane potential. Because of a continuous leak, Na(+) has to be permanently removed from the intracellular milieu, a process that is thought to be exclusively mediated by the Na(+)/K(+)-ATPase in animal cells. Here, we show that intercalated cells of the mouse kidney are an exception to this general rule. By an approach combining two-photon imaging of isolated renal tubules, physiological studies, and genetically engineered animals, we demonstrate that inhibition of the H(+) vacuolar-type ATPase (V-ATPase) caused drastic cell swelling and depolarization, and also inhibited the NaCl absorption pathway that we recently discovered in intercalated cells. In contrast, pharmacological blockade of the Na(+)/K(+)-ATPase had no effects. Basolateral NaCl exit from β-intercalated cells was independent of the Na(+)/K(+)-ATPase but critically relied on the presence of the basolateral ion transporter anion exchanger 4. We conclude that not all animal cells critically rely on the sodium pump as the unique bioenergizer, but can be replaced by the H(+) V-ATPase in renal intercalated cells. This concept is likely to apply to other animal cell types characterized by plasma membrane expression of the H(+) V-ATPase.
authors.affiliation: Institut National de la Santé et de la Recherche Médicale, Unité Mixte de Recherche de Santé 872, Centre de Recherche Paris Centre de Recherche des Cordeliers, Faculté de Médecine Paris Descartes, Sorbonne Paris Cité, F-75006 Paris, France.
authors.forename: Régine
authors.initials: R
authors.lastname: Chambrey
authors.forename: Ingo
authors.initials: I
authors.lastname: Kurth
authors.forename: Janos
authors.initials: J
authors.lastname: Peti-Peterdi
authors.forename: Pascal
authors.initials: P
authors.lastname: Houillier
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Purkerson
authors.forename: Françoise
authors.initials: F
authors.lastname: Leviel
authors.forename: Moritz
authors.initials: M
authors.lastname: Hentschke
authors.forename: Anselm A
authors.initials: AA
authors.lastname: Zdebik
authors.forename: George J
authors.initials: GJ
authors.lastname: Schwartz
authors.forename: Christian A
authors.initials: CA
authors.lastname: Hübner
authors.forename: Dominique
authors.initials: D
authors.lastname: Eladari
mesh_terms: D000042:Absorption; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D027963:Chloride-Bicarbonate Antiporters / Q000235:genetics; D007150:Immunohistochemistry; D007477:Ions; D007668:Kidney / Q000378:metabolism*; D008564:Membrane Potentials; D051379:Mice; D018345:Mice, Knockout; D010477:Perfusion; D017494:Proton Pumps / Q000502:physiology; D012964:Sodium / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism / Q000502:physiology*; D025262:Vacuolar Proton-Translocating ATPases / Q000378:metabolism
chemical_list: D027963:Chloride-Bicarbonate Antiporters; D007477:Ions; D017494:Proton Pumps; C480025:Slc4a9 protein, mouse; D012965:Sodium Chloride; D012964:Sodium; D025262:Vacuolar Proton-Translocating ATPases; D000254:Sodium-Potassium-Exchanging ATPase
keywords: ion transporter; plasma membrane; proton pump