title: Visualization of brain circuits using two-photon fluorescence micro-optical sectioning tomography.
abstract: Neural circuits are fundamental for brain functions. However, obtaining long range continuous projections of neurons in the entire brain is still challenging. Here a two-photon fluorescence micro-optical sectioning tomography (2p-fMOST) method is developed for high-throughput, high-resolution visualization of the brain circuits. Two-photon imaging technology is used to obtain high resolution, and acoustical optical deflector (AOD), an inertia-free beam scanner is used to realize fast and prolonged stable imaging. The combination of these techniques with imaging and then sectioning method of a plastic-embedded mouse brain facilitated the acquisition of a three-dimensional data set of a fluorescent mouse brain with a resolution adequate to resolve the spines. In addition, the brain circuit tracing ability is showed by several neurons projecting across different brain regions. Besides brain imaging, 2p-fMOST could be used in many studies that requires sub-micro resolution or micro resolution imaging of a large sample.
authors.affiliation: Britton Chance Center for Biomedical Photonics, Huazhong University of Science and Technology-Wuhan National Laboratory for Optoelectronics, Wuhan 430074, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zheng
authors.forename: Zhongqing
authors.initials: Z
authors.lastname: Yang
authors.forename: Anan
authors.initials: A
authors.lastname: Li
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Lv
authors.forename: Zhenqiao
authors.initials: Z
authors.lastname: Zhou
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Wang
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Qi
authors.forename: Shiwei
authors.initials: S
authors.lastname: Li
authors.forename: Qingming
authors.initials: Q
authors.lastname: Luo
authors.forename: Hui
authors.initials: H
authors.lastname: Gong
authors.forename: Shaoqun
authors.initials: S
authors.lastname: Zeng
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation*; D009415:Nerve Net / Q000166:cytology*; D041622:Tomography, Optical / Q000295:instrumentation*