title: Seleniivibrio woodruffii gen. nov., sp. nov., a selenate- and arsenate-respiring bacterium in the Deferribacteraceae.
abstract: A Gram-type-negative, obligately anaerobic, selenate-respiring bacterium, strain S4(T), was isolated from activated sludge of a wastewater treatment plant in New Jersey after enrichment with 10 mM selenate as the sole electron acceptor. In addition to its selenate-respiring capability, strain S4(T) also respired arsenate with acetate as carbon source and electron donor. Fermentative growth was not observed. The optimum growth temperature was 37 °C and optimum pH was pH 7. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain S4(T) is a novel member of the family Deferribacteraceae, with the type strain of Denitrovibrio acetiphilus as its closest cultivated relative, with 91.5 % sequence similarity. The cellular fatty acid profile was composed predominantly of straight-chain fatty acids C14 : 0, C15 : 0, C16 : 0, C17 : 0 and C18 : 0, which distinguishes this organism from its closest relatives. The DNA G+C content was 47.7 mol%. Together, these findings support the conclusion that strain S4(T) represents a novel genus and species, for which the name Seleniivibrio woodruffii gen. nov., sp. nov. is proposed. The type strain of Seleniivibrio woodruffii is S4(T) ( = DSM 24984(T) = ATCC BAA-2290(T)).
authors.affiliation: Rutgers University, Department of Biochemistry and Microbiology, School of Environmental and Biological Sciences, 76 Lipman Drive, New Brunswick, NJ 08901, USA.
authors.forename: Ines
authors.initials: I
authors.lastname: Rauschenbach
authors.affiliation: Rutgers University, Department of Biochemistry and Microbiology, School of Environmental and Biological Sciences, 76 Lipman Drive, New Brunswick, NJ 08901, USA.
authors.forename: Valeriya
authors.initials: V
authors.lastname: Posternak
authors.affiliation: Rutgers University, Department of Biochemistry and Microbiology, School of Environmental and Biological Sciences, 76 Lipman Drive, New Brunswick, NJ 08901, USA.
authors.forename: Pasquale
authors.initials: P
authors.lastname: Cantarella
authors.affiliation: Rutgers University, Department of Biochemistry and Microbiology, School of Environmental and Biological Sciences, 76 Lipman Drive, New Brunswick, NJ 08901, USA.
authors.forename: Jennifer
authors.initials: J
authors.lastname: McConnell
authors.affiliation: Rutgers University, Department of Cell Biology and Neuroscience, 604 Allison Road, Piscataway, NJ 08854, USA.
authors.forename: Valentin
authors.initials: V
authors.lastname: Starovoytov
authors.affiliation: Rutgers University, Department of Biochemistry and Microbiology, School of Environmental and Biological Sciences, 76 Lipman Drive, New Brunswick, NJ 08901, USA.
authors.forename: Max M
authors.initials: MM
authors.lastname: Häggblom
mesh_terms: D001149:Arsenates / Q000378:metabolism*; D001421:Bacteria, Anaerobic / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006090:Gram-Negative Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D009515:New Jersey; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D064586:Selenic Acid / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D062065:Wastewater / Q000382:microbiology
chemical_list: D001149:Arsenates; D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D064586:Selenic Acid; D012722:Sewage; D062065:Wastewater; C025657:arsenic acid