title: Characterization of electron transfer dissociation in the Orbitrap Velos HCD cell.
abstract: Electron transfer dissociation (ETD) is commonly employed in ion traps utilizing rf fields that facilitate efficient electron transfer reactions. Here, we explore performing ETD in the HCD collision cell on an Orbitrap Velos instrument by applying a static DC gradient axially to the rods. This gradient enables simultaneous three dimensional, charge sign independent, trapping of cations and anions, initiating electron transfer reactions in the center of the HCD cell where oppositely charged ions clouds overlap. Here, we evaluate this mode of operation for a number of tryptic peptide populations and the top-down sequence analysis of ubiquitin. Our preliminary data show that performing ETD in the HCD cell provides similar fragmentation as ion trap-ETD but requires further optimization to match performance of ion trap-ETD.
authors.affiliation: Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, 3584 CH, Utrecht, The Netherlands.
authors.forename: Christian K
authors.initials: CK
authors.lastname: Frese
authors.forename: Dirk
authors.initials: D
authors.lastname: Nolting
authors.forename: A F Maarten
authors.initials: AF
authors.lastname: Altelaar
authors.forename: Jens
authors.initials: J
authors.lastname: Griep-Raming
authors.forename: Shabaz
authors.initials: S
authors.lastname: Mohammed
authors.forename: Albert J R
authors.initials: AJ
authors.lastname: Heck
mesh_terms: D002412:Cations; D004583:Electrons; D006367:HeLa Cells; D006801:Humans; D007477:Ions / Q000737:chemistry; D013058:Mass Spectrometry / Q000295:instrumentation*; D010446:Peptide Fragments / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000295:instrumentation; D025801:Ubiquitin / Q000737:chemistry
chemical_list: D002412:Cations; D007477:Ions; D010446:Peptide Fragments; D010455:Peptides; D025801:Ubiquitin