title: 8OHdG is not a biomarker for Huntington disease state or progression.
abstract: OBJECTIVE
  To evaluate plasma 8-hydroxy-deoxy-guanosine (8OHdG) levels as a potential biomarker of premanifest and early Huntington disease (HD).
  
  
  METHODS
  Personnel from 2 independent laboratories quantified 8OHdG in blinded longitudinal plasma samples taken 24 months apart from 160 TRACK-HD participants, as well as samples containing control plasma with added ("spiked") 8OHdG. One laboratory used a liquid chromatography-electrochemical array (LCECA) assay, and the other used liquid chromatography-mass spectrometry (LCMS).
  
  
  RESULTS
  The LCMS assay was more accurate than the LCECA assay for measurements of "spiked" 8OHdG levels in plasma. Neither assay demonstrated cross-sectional differences in plasma 8OHdG among controls, premanifest HD, and early symptomatic HD. Similarly, neither assay showed longitudinal changes in any disease group over 24 months.
  
  
  CONCLUSIONS
  Plasma concentration of 8OHdG is not a biomarker of disease state or progression in HD. We recommend that future putative biomarker studies use blinded sample analysis, standard curves, independent analytical methods, and strict quality control of sample collection and storage.
authors.affiliation: CHDI Management/CHDI Foundation, Princeton, NJ, USA. beth.borowsky@chdifoundation.org
authors.forename: Beth
authors.initials: B
authors.lastname: Borowsky
authors.forename: John
authors.initials: J
authors.lastname: Warner
authors.forename: Blair R
authors.initials: BR
authors.lastname: Leavitt
authors.forename: Sarah J
authors.initials: SJ
authors.lastname: Tabrizi
authors.forename: Raymund A C
authors.initials: RA
authors.lastname: Roos
authors.forename: Alexandra
authors.initials: A
authors.lastname: Durr
authors.forename: Chris
authors.initials: C
authors.lastname: Becker
authors.forename: Cristina
authors.initials: C
authors.lastname: Sampaio
authors.forename: Allan J
authors.initials: AJ
authors.lastname: Tobin
authors.forename: Howard
authors.initials: H
authors.lastname: Schulman
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D015415:Biomarkers / Q000097:blood; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000097:blood; D018450:Disease Progression; D005260:Female; D006801:Humans; D006816:Huntington Disease / Q000097:blood / Q000175:diagnosis* / Q000473:pathology*; D008137:Longitudinal Studies; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D016037:Single-Blind Method
chemical_list: D015415:Biomarkers; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine