title: Production of zearalenone-4-glucoside, a-zearalenol-4-glucoside and ß-zearalenol-4-glucoside.
abstract: The work at hand describes the production of the zearalenone (ZON) metabolites zearalenone-4-glucoside (ZON-4G), a-zearalenol-4-glucoside (oc-ZOL-4G) and ß-zearalenol-4-glucoside (ß-ZOL-4G). In a first step a genetically modified yeast strain, expressing theArabidopsis thaliana UDP-glu-cosyltransferase UGT73C6, was treated with ZON to produce ZON-4G. The substance was purified by solid phase extraction and subsequent reversed phase preparative HPLC prior to the reduction with sodium borohydride to yield 0C-ZOL-4G and ß-ZOL-4G. The identity and purity of the substances were confirmed by(13)C-and(1)H-NMR as well as by HPLC-UV. In total, 50 mg of ZON were used to produce 5 mg of a-ZOL-4G with a purity of 98%, 6 mg of ß-ZOL-4G with a purity of 99% and 5 mg of ZON-4G with a purity of 99%.
authors.affiliation: Christian Doppler Laboratory for Mycotoxin Research, Dept. for Agrobiotechnology (IFA-Tulln), University of Natural Resources and Applied Life Sciences Vienna, Konrad Lorenz Str. 20, 3430, Tulln, Austria.
authors.forename: P
authors.initials: P
authors.lastname: Krenn
authors.forename: F
authors.initials: F
authors.lastname: Berthiller
authors.forename: W
authors.initials: W
authors.lastname: Schweiger
authors.forename: C
authors.initials: C
authors.lastname: Hametner
authors.forename: R
authors.initials: R
authors.lastname: Ludwig
authors.forename: G
authors.initials: G
authors.lastname: Adam
authors.forename: R
authors.initials: R
authors.lastname: Krska
authors.forename: R
authors.initials: R
authors.lastname: Schuhmacher