title: Proteomic analysis of brain mitochondrial proteome and mitochondrial complexes.
abstract: We describe various complementary techniques to achieve multidimensional mitochondrial proteome fractionation and analysis. Previously described methods for 2D-DIGE/mass spectrometry and 1D-SDS-PAGE/Western techniques and protein complex analysis by BN-PAGE/Western and sucrose gradient ultracentrifugation/SDS-PAGE/mass spectrometry are optimized to characterize the brain mitochondrial proteome. This approach allows for a comprehensive identification of mitochondrial proteomic differences between health and disease conditions.
authors.affiliation: Department of Biochemistry, Institute for Biomolecular Design, University of Alberta, Edmonton, AB, Canada.
authors.forename: Ana
authors.initials: A
authors.lastname: Lopez-Campistrous
authors.forename: Carlos
authors.initials: C
authors.lastname: Fernandez-Patron
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D001923:Brain Chemistry; D002499:Centrifugation, Density Gradient; D005591:Chemical Fractionation; D004591:Electrophoresis, Polyacrylamide Gel; D015870:Gene Expression; D006801:Humans; D007525:Isoelectric Focusing; D008928:Mitochondria / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010449:Peptide Mapping / Q000379:methods*; D059748:Proteolysis; D020543:Proteome / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D051381:Rats; D053719:Tandem Mass Spectrometry
chemical_list: D020543:Proteome