title: The evolution of simulation techniques for dynamic bone tissue engineering in bioreactors.
abstract: Bone tissue engineering aims to overcome the drawbacks of current bone regeneration techniques in orthopaedics. Bioreactors are widely used in the field of bone tissue engineering, as they help support efficient nutrition of cultured cells with the possible combination of applying mechanical stimuli. Beneficial influencing parameters of in vitro cultures are difficult to find and are mostly determined by trial and error, which is associated with significant time and money spent. Mathematical simulations can support the finding of optimal parameters. Simulations have evolved over the last 20 years from simple analytical models to complex and detailed computational models. They allow researchers to simulate the mechanical as well as the biological environment experienced by cells seeded on scaffolds in a bioreactor. Based on the simulation results, it is possible to give recommendations about specific parameters for bone bioreactor cultures, such as scaffold geometries, scaffold mechanical properties, the level of applied mechanical loading or nutrient concentrations. This article reviews the evolution in simulating various aspects of dynamic bone culture in bioreactors and reveals future research directions.
authors.affiliation: Institute for Biomechanics, Swiss Federal Institute of Technology Zürich (ETHZ), Switzerland.
authors.forename: Jolanda Rita
authors.initials: JR
authors.lastname: Vetsch
authors.affiliation: Institute for Biomechanics, Swiss Federal Institute of Technology Zürich (ETHZ), Switzerland.
authors.forename: Ralph
authors.initials: R
authors.lastname: Müller
authors.affiliation: Institute for Biomechanics, Swiss Federal Institute of Technology Zürich (ETHZ), Switzerland.
authors.forename: Sandra
authors.initials: S
authors.lastname: Hofmann
mesh_terms: D019149:Bioreactors; D001861:Bone Regeneration; D001842:Bone and Bones / Q000473:pathology*; D002478:Cells, Cultured; D003198:Computer Simulation; D004867:Equipment Design; D005109:Extracellular Matrix / Q000378:metabolism; D006801:Humans; D008962:Models, Theoretical; D009985:Orthopedics; D010006:Osteoblasts / Q000166:cytology; D010477:Perfusion; D015203:Reproducibility of Results; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
keywords: bioreactor; bone; dynamic tissue engineering; mechanical stimuli; scaffold; simulation