title: PNA-assembled graphene oxide for sensitive and selective detection of DNA.
abstract: DNA detection based on peptide nucleic acid (PNA)-DNA hybridization is emerging as an important method in the area of DNA microarrays and biosensors because PNA shows remarkable hybridization properties. In this work, we provide a novel, simple, sensitive, and selective strategy based on a PNA-graphene oxide (GO) assembled biosensor for fluorescence turn-on detection of DNA, in which the new nanomaterial GO was used as a scaffold for PNA and a quencher for the fluorophore. The PNA-GO assembled biosensor is capable of distinguishing sequence specificity including complementary, one-base mismatched and non-complementary targets. Moreover, the results show that the biosensor is able to detect target DNA down to hundreds of picomolar. This sensing platform has been demonstrated to be highly sensitive and specific, and we expect that it will find great applications in the field of biomedicine and disease diagnostics.
authors.affiliation: School of Laboratory Medicine, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Guo
authors.forename: Danxin
authors.initials: D
authors.lastname: Du
authors.forename: Lina
authors.initials: L
authors.lastname: Tang
authors.forename: Yong
authors.initials: Y
authors.lastname: Ning
authors.forename: Qunfeng
authors.initials: Q
authors.lastname: Yao
authors.forename: Guo-Jun
authors.initials: GJ
authors.lastname: Zhang
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D006108:Graphite / Q000737:chemistry*; D007700:Kinetics; D057230:Limit of Detection; D010087:Oxides / Q000737:chemistry*; D020135:Peptide Nucleic Acids / Q000737:chemistry* / Q000235:genetics; D013050:Spectrometry, Fluorescence; D013696:Temperature
chemical_list: D010087:Oxides; D020135:Peptide Nucleic Acids; D006108:Graphite; D004247:DNA