title: [Analysis on pathogen detection for 53 influenza outbreaks].
abstract: OBJECTIVE
  In order to provide a scientific basis for influenza prevention and control, analyzing the epidemic characteristics and laws of influenza outbreaks in Xuzhou area during 2005-2011.
  
  
  METHODS
  Using fluorescent-PCR method to detect influenza virus nucleic acid on Nasopharyngeal swab specimens collected from influenza outbreak cases during 2005-2011 and fast classifying influenza virus A1 (H1N1), A3 (H3N2), new H1N1 BV (Victoria) and BY (Yamagate) on subtypes. At the same time, isolating the influenza virus with MDCK cells, and sending them to the National Influenza Center for review, after the preliminary identification of the isolated influenza virus.
  
  
  RESULTS
  During 2005-2011, there are 53 influenza outbreaks in Xuzhou area, which caused by influenza virus subtype BV accounting for 26.42% (14/53), A3 accounting for 49.1% (26/53), A3 and A1 mixture accounting for 3.77% (2/53) and the new H1N1 accounting for 20.75% (11/53). The outbreaks in 2007 and 2009 mainly caused by A3, and show that the winter spring (January) and summer autumn (September) as two popular peaks during 2005-2011; BV mainly causes the outbreaks from Feb. to Jun.
  
  
  CONCLUSIONS
  In Xuzhou area, since the winter of 2005, influenza virus subtype BV, the A3, and new H1N1 has alternately as mainly predominant strain, caused local influenza outbreaks. In which BV has increased trend year by year during 2005-2011. The students in primary and secondary schools are the major crowd of influenza outbreaks. Fluorescent-PCR detection methods could be a preferred method for reliable and rapid diagnostic of epidemic influenza outbreaks.
authors.affiliation: Xuzhou Disease Control and Prevention Center, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Tong
authors.forename: Jin-Chuan
authors.initials: JC
authors.lastname: Yang
authors.forename: Chuan-Ling
authors.initials: CL
authors.lastname: Zhang
authors.forename: Bao-An
authors.initials: BA
authors.lastname: Wang
authors.forename: Ren
authors.initials: R
authors.lastname: Ding
authors.forename: Yang-Guang
authors.initials: YG
authors.lastname: Du
authors.forename: Jia-Lu
authors.initials: JL
authors.lastname: Yan
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Liu-Ying
authors.initials: LY
authors.lastname: Tang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D004196:Disease Outbreaks; D005260:Female; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D053122:Influenza A Virus, H3N2 Subtype / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D009980:Influenza A virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D007251:Influenza, Human / Q000453:epidemiology* / Q000821:virology*; D008297:Male; D008875:Middle Aged; D062486:Public Health Surveillance; D012621:Seasons; D055815:Young Adult