title: Endogenous abscisic acid is involved in methyl jasmonate-induced reactive oxygen species and nitric oxide production but not in cytosolic alkalization in Arabidopsis guard cells.
abstract: We recently demonstrated that endogenous abscisic acid (ABA) is involved in methyl jasmonate (MeJA)-induced stomatal closure in Arabidopsis thaliana. In this study, we investigated whether endogenous ABA is involved in MeJA-induced reactive oxygen species (ROS) and nitric oxide (NO) production and cytosolic alkalization in guard cells using an ABA-deficient Arabidopsis mutant, aba2-2, and an inhibitor of ABA biosynthesis, fluridon (FLU). The aba2-2 mutation impaired MeJA-induced ROS and NO production. FLU inhibited MeJA-induced ROS production in wild-type guard cells. Pretreatment with 0.1 μM ABA, which does not induce stomatal closure in the wild type, complemented the insensitivity to MeJA of the aba2-2 mutant. However, MeJA induced cytosolic alkalization in both wild-type and aba2-2 guard cells. These results suggest that endogenous ABA is involved in MeJA-induced ROS and NO production but not in MeJA-induced cytosolic alkalization in Arabidopsis guard cells.
authors.affiliation: Graduate School of Natural Science and Technology, Okayama University, 1-1-1 Tsushima-Naka, Okayama 700-8530, Japan.
authors.forename: Wenxiu
authors.initials: W
authors.lastname: Ye
authors.forename: Mohammad Anowar
authors.initials: MA
authors.lastname: Hossain
authors.forename: Shintaro
authors.initials: S
authors.lastname: Munemasa
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Izumi C
authors.initials: IC
authors.lastname: Mori
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Murata
mesh_terms: D000040:Abscisic Acid / Q000235:genetics / Q000378:metabolism*; D000085:Acetates / Q000378:metabolism; D017360:Arabidopsis / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D003517:Cyclopentanes / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism*; D006540:Herbicides / Q000494:pharmacology*; D009154:Mutation; D009569:Nitric Oxide / Q000378:metabolism*; D054883:Oxylipins / Q000378:metabolism; D054046:Plant Stomata / Q000378:metabolism; D011728:Pyridones / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D006540:Herbicides; D054883:Oxylipins; D011728:Pyridones; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; C013351:fluridone; D000040:Abscisic Acid; C072239:methyl jasmonate
keywords: 2′,7′-bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester; 2′,7′-dichlorodihydrofluorescein diacetate; 4,5-diaminofluorescein-2 diacetate; ABA; Arabidopsis thaliana; BCECF-AM; Cytosolic alkalization; DAF-2DA; FLU; H(2)DCF-DA; MeJA; Methyl jasmonate; Nitric oxide; ROS; Reactive oxygen species; [Ca(2+)](cyt); abscisic acid; cytosolic free calcium concentration; cytosolic pH; fluridon; methyl jasmonate; pH(cyt); reactive oxygen species