title: Inhibition of store-operated Ca2+ entry suppresses EGF-induced migration and eliminates extravasation from vasculature in nasopharyngeal carcinoma cell.
abstract: Store-operated Ca(2+) entry (SOCE) mediates Ca(2+) responses evoked by extracellular signaling molecules to promote increases in cytosolic Ca(2+), thereby triggering downstream signal transduction. Here we demonstrated that either the pharmacological blockage of Ca(2+) influx through SOCE or the knockdown of Orai1, a key molecule of SOCE, suppressed the epidermal growth factor-induced migration by disturbing Ca(2+) signaling in nasopharyngeal carcinoma (NPC) cell. Furthermore, Orai1 depletion led to a delayed cell attachment to the extracellular matrix surface in vitro and eliminated the extravasation of microinjected cells from vasculature in a zebrafish hematogenous metastasis model. Our findings thus indicate that SOCE acts as a predominant Ca(2+) signaling involved in NPC cell metastasis, and may serve as a candidate target for anti-metastasis therapy in NPC.
authors.affiliation: Medical Photonics Research Center, Hamamatsu University School of Medicine, 1-20-1 Handayama, Hamamatsu 431-3192, Japan.
authors.forename: Jinyan
authors.initials: J
authors.lastname: Zhang
authors.forename: Jiazhang
authors.initials: J
authors.lastname: Wei
authors.forename: Masamitsu
authors.initials: M
authors.lastname: Kanada
authors.forename: Libo
authors.initials: L
authors.lastname: Yan
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhang
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Watanabe
authors.forename: Susumu
authors.initials: S
authors.lastname: Terakawa
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002118:Calcium / Q000378:metabolism*; D002121:Calcium Channel Blockers / Q000494:pharmacology; D020013:Calcium Signaling / Q000187:drug effects / Q000502:physiology*; D002277:Carcinoma; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D004815:Epidermal Growth Factor / Q000378:metabolism*; D006801:Humans; D052139:Intracellular Calcium-Sensing Proteins; D017136:Ion Transport; D008565:Membrane Proteins / Q000378:metabolism*; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D015398:Signal Transduction; D015027:Zebrafish
chemical_list: D002121:Calcium Channel Blockers; D052139:Intracellular Calcium-Sensing Proteins; D008565:Membrane Proteins; C572578:SARAF protein, human; D004815:Epidermal Growth Factor; D002118:Calcium