title: Biochemical characterization of 4-α-glucanotransferase from Saccharophagus degradans 2-40 and its potential role in glycogen degradation.
abstract: 4-α-Glucanotransferase, an enzyme encoded by malQ, transfers 1,4-α-glucan to an acceptor carbohydrate to produce long linear maltodextrins of varying lengths. To investigate the biochemical characteristics of the malQ gene (Sde0986) from Saccharophagus degradans 2-40 and to understand its physiological role in vivo, the malQ gene was cloned and expressed in Escherichia coli. The amino acid sequence of MalQ was found to be 36-47% identical to that of amylomaltases from gammaproteobacteria. MalQ is a monomeric enzyme that belongs to a family of 77 glycoside hydrolases, with a molecular mass of 104 kDa. The optimal pH and temperature for MalQ toward maltotriose were determined to be 8.5 and 35 °C, respectively. Furthermore, the enzyme displayed glycosyl transfer activity on maltodextrins of various sizes to yield glucose and long linear maltodextrins. MalQ, however, could be distinguished from other bacterial and archaeal amylomaltases in that it did not produce maltose and cyclic glucan. Reverse transcription PCR results showed that malQ was not induced by maltose and was highly expressed in the stationary phase. These data suggest that the main physiological role of malQ in S. degradans is in the degradation of glycogen, although the gene is commonly known to be involved in maltose metabolism in E. coli.
authors.affiliation: The Microbiological Resource Research Institute, Pusan National University, Busan, Korea.
authors.forename: Sungmin
authors.initials: S
authors.lastname: Hwang
authors.forename: Kyoung-Hwa
authors.initials: KH
authors.lastname: Choi
authors.forename: Jieun
authors.initials: J
authors.lastname: Kim
authors.forename: Jaeho
authors.initials: J
authors.lastname: Cha
mesh_terms: D034241:Alteromonadaceae / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D004795:Enzyme Stability; D006003:Glycogen / Q000378:metabolism*; D006004:Glycogen Debranching Enzyme System / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016415:Sequence Alignment
chemical_list: D001426:Bacterial Proteins; D006004:Glycogen Debranching Enzyme System; D006003:Glycogen; C020569:4 alpha-glucanotransferase