title: [Species differentiation and quality assessment of Ziziphus jujuba var. spinosa based on the HPLC fingerprint and quantitative analysis].
abstract: OBJECTIVE
  To establish an HPLC method of a characteristical chemical fingerprint analysis in combination with simultaneous determination of four bioactive components for species differentiation and quality assessment of Ziziphus jujuba var. spinosa.
  
  
  METHODS
  The chromatographic separation was performed on an Agilent TC-C18 BDS (250 mm x 4.6 mm, 5 microm) column. The mobile phase consisted of acetonitrile and water in a linear gradient elution procedure. The evaporator tube temperature of ELSD was set at 110.5 degrees C with the nebulizing gas flow rate of 3.1 mL/min and the flow rate of mobile phase was 1.0 mL/min. The column was maintained at 30 degrees C. The injection volume was 20 microL.
  
  
  RESULTS
  HPLC methodology for both chemical fingerprint analysis and quantitative determination of four active ingredients were validated, respectively. According to the contents of the four ingredients and the chemical fingerprints of Ziziphus jujuba var. spinosa using principal component analysis, Ziziphus jujuba var. spinosa was different from the fake derived from the seeds of Ziziphus mauritiana.
  
  
  CONCLUSIONS
  The developed HPLC method is exclusive and repetitive for the species identification and quality evaluation of Ziziphus jujuba var. spinosa.
authors.affiliation: Guang'anmen hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China. zhangcy727@yahoo.com.cn
authors.forename: Cui-Ying
authors.initials: CY
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Guo
authors.forename: Hui-Ping
authors.initials: HP
authors.lastname: Cheng
authors.forename: Han-Ming
authors.initials: HM
authors.lastname: Cui
authors.forename: Ping
authors.initials: P
authors.lastname: Wu
authors.forename: Yu
authors.initials: Y
authors.lastname: Dong
authors.forename: Shi-Jing
authors.initials: SJ
authors.lastname: Huang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004340:Drug Contamination; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000592:standards; D005638:Fruit / Q000737:chemistry; D013287:Illicit Drugs; D053978:Pentacyclic Triterpenes; D025341:Principal Component Analysis; D011786:Quality Control; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis*; D012639:Seeds / Q000737:chemistry*; D014315:Triterpenes / Q000032:analysis; D031957:Ziziphus / Q000737:chemistry* / Q000145:classification; D000094062:Betulinic Acid
chemical_list: D004365:Drugs, Chinese Herbal; D013287:Illicit Drugs; D053978:Pentacyclic Triterpenes; D012503:Saponins; D014315:Triterpenes; C540008:jujuboside B; C087808:jujuboside A; D000094062:Betulinic Acid