title: [PCR-SSCP molecular identification of Panax ginseng and P. quinquefolius based on ITS2 bar coding SNPs].
abstract: OBJECTIVE
  To establish a method for identifying Panax ginseng and P. quinquefolius with PCR-SSCP, on the basis of specific SNP identification sites of their ITS2 bar codes.
  
  
  METHODS
  ITS2 sequences of P. ginseng and P. quinquefolius recorded in GenBank were searched to conduct a comparative analysis and screen out specific SNP identification sites of their ITS2 bar codes. Based on that, the Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP) method was adopted for analyzing 11 P. ginseng samples and 10 P. quinquefolius samples and verifying sequencing of their PCR products.
  
  
  RESULTS
  The P. ginseng and P. quinquefolius samples had the same agarose mages of PCR-SSCP with the standard medicines. There were significant differences between the PCR-SSCP agarose mages of P. ginseng and P. quinquefolius, with identifical identification results between PCR-SSCP and sequencing.
  
  
  CONCLUSIONS
  Compared with the sequencing method, PCR-SSCP is so rapid and accurate that it can be used for identifying P. ginseng and P. quinquefolius medicines.
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. zhanxjhzl@163.com
authors.forename: Xin-Jie
authors.initials: XJ
authors.lastname: Zhan
authors.forename: Cheng
authors.initials: C
authors.lastname: Tian
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Chun-Sheng
authors.initials: CS
authors.lastname: Liu
mesh_terms: D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000032:analysis / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D005894:Panax / Q000145:classification / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational; D015203:Reproducibility of Results; D013045:Species Specificity
chemical_list: D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer