title: PolyNaSS grafting on titanium surfaces enhances osteoblast differentiation and inhibits Staphylococcus aureus adhesion.
abstract: Titanium surface modifications to simultaneously prevent bacterial adhesion but promote bone-cell functions could be highly beneficial for improving implant osseointegration. In the present in vitro study, the effect of sulfonate groups on titanium surfaces was investigated with respect to both S. aureus adhesion and osteoblast functions pertinent to new bone formation. Commercial pure titanium (cpTi) squares were oxydized (Tiox), grafted with poly(sodium styrene sulfonate) groups (Tigraft) by covalent bonding using radical polymerization, and were characterized by infrared spectroscopy (HATR-FTIR) and colorimetry. Bacterial adhesion study showed that Tigraft exhibited high inhibition of S. aureus adhesion S at levels >90 %, when compared to cpTi (P < 0.05). In contrast osteoblasts adhesion was similar on all three titanium surfaces. While the kinetics of cell proliferation were similar on the three titanium surfaces, Alkaline phosphatase-specific activity of osteoblasts cultured on Tigraft surfaces was twofold higher than that observed on either on Tiox or cpTi surfaces (P < 0.01). More importantly, the amount and the distribution of calcium-containing nodules was different. The total area covered by calcium-containing nodules was 2.2-fold higher on the Tigraft as compared to either Tiox or cpTi surfaces (P < 0.01). These results provide evidence that poly(sodium styrene sulfonate) groups grafting on cpTi simultaneously inhibits bacteria adhesion but promote osteoblast function pertinent to new bone formation. Such modified titanium surfaces offer a promising strategy for preventing biofilm-related infections and enhancing osteointegration of implants in orthopaedic and dental applications.
authors.affiliation: Laboratoire de Bio ingénierie et Biomécanique Ostéoarticulaires, UMR CNRS 7052, 10, Avenue de Verdun, 75010, Paris, France.
authors.forename: A
authors.initials: A
authors.lastname: Alcheikh
authors.forename: G
authors.initials: G
authors.lastname: Pavon-Djavid
authors.forename: G
authors.initials: G
authors.lastname: Helary
authors.forename: H
authors.initials: H
authors.lastname: Petite
authors.forename: V
authors.initials: V
authors.lastname: Migonney
authors.forename: F
authors.initials: F
authors.lastname: Anagnostou
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects*; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D004622:Embryo, Mammalian; D008422:Materials Testing; D008826:Microbial Sensitivity Tests; D016348:Osseointegration / Q000187:drug effects; D010006:Osteoblasts / Q000187:drug effects* / Q000502:physiology; D010012:Osteogenesis / Q000187:drug effects; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D011137:Polystyrenes / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012964:Sodium / Q000737:chemistry / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000502:physiology; D013452:Sulfonium Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology
chemical_list: D020099:Coated Materials, Biocompatible; D011108:Polymers; D011137:Polystyrenes; D013452:Sulfonium Compounds; C024075:styrofoam; D012964:Sodium; D014025:Titanium