title: Arginase II inhibitory activity of flavonoid compounds from Scutellaria indica.
abstract: Arginase II has recently reported as a novel therapeutic target for the treatment of cardiovascular diseases such as atherosclerosis. In the course of screening plants used in natural medicines as arginase II inhibitory activity, a methanol extract of Scutellaria indica showed significant inhibitory effect. Further fractionation and repeated column chromatography led to the isolation of a new flavan-type (1), and seven known compounds (2-8). The chemical structures of isolated compounds were elucidated based on extensive 1D and 2D NMR spectroscopic data. The isolates 1-8 were investigated in vitro for their arginase II inhibitory activity using enzyme solution prepared from kidney of anesthetized C57BL/6 mice. Compounds 3 and 5 significantly inhibited arginase II activity with IC₅₀ values of 25.1 and 11.6 μM, respectively, whereas the other compounds were apparently inactive.
authors.affiliation: College of Pharmacy, Catholic University of Daegu, Gyeongsan, Gyeongbuk 712-702, Republic of Korea.
authors.forename: Sang Won
authors.initials: SW
authors.lastname: Kim
authors.forename: To Dao
authors.initials: TD
authors.lastname: Cuong
authors.forename: Tran Manh
authors.initials: TM
authors.lastname: Hung
authors.forename: Sungwoo
authors.initials: S
authors.lastname: Ryoo
authors.forename: Jeong Hyung
authors.initials: JH
authors.lastname: Lee
authors.forename: Byung Sun
authors.initials: BS
authors.lastname: Min
mesh_terms: D000818:Animals; D001119:Arginase / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D005419:Flavonoids / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D031363:Scutellaria / Q000201:enzymology*
chemical_list: D004791:Enzyme Inhibitors; D005419:Flavonoids; D010936:Plant Extracts; C481673:Arg2 protein, mouse; D001119:Arginase