title: [Determination of DON-3-Glucoside in artificially and naturally contaminated wheat with LC-MS/MS].
abstract: Naturally contaminated and artificiallyFusarium spp. inoculated wheat was analyzed for deoxynivalenol, deoxynivalenol-3-glucoside, 3-acetyl-deoxynivalenol and 15-acetyl-deoxynivalenol. After extraction and clean-up with MycoSep columns, the trichothecenes were determined using a LC-ESI-MS/MS method. Deoxynivalenol-3-glucoside was detectable in 4 out of 4 artificially inoculated and in 22 out of 25 naturally contaminated wheat samples. For the latter, the average relative deoxynivalenol-3-glucoside concentration was about 6% of the deoxynivalenol concentration. The maximum relative deoxynivalenol-3-glucoside concentration was 12% as compared to the concentration of deoxynivalenol. In all samples, the concentration of deoxynivalenol-3-glucoside was higher than the concentrations of 3-acetyl-deoxynivalenol or 15-acetyl-deoxynivalenol.
authors.affiliation: Christian Doppler Laboratory for Mycotoxin Research, Department for Agrobiotechnology (IFA-Tulln), University of Natural Resources and Applied Life Sciences, Vienna, Konrad Lorenz Strasse 20, 3430, Tulln, Austria.
authors.forename: F
authors.initials: F
authors.lastname: Berthiller
authors.forename: R
authors.initials: R
authors.lastname: Krska
authors.forename: C
authors.initials: C
authors.lastname: Dall'asta
authors.forename: M
authors.initials: M
authors.lastname: Lemmens
authors.forename: G
authors.initials: G
authors.lastname: Adam
authors.forename: R
authors.initials: R
authors.lastname: Schuhmacher