title: Interleukin-10 Serum Levels after Vaccination with In Vivo Prepared Toxoplasma gondii Excreted/Secreted Antigens.
abstract: OBJECTIVE
  Toxoplasma gondii is a worldwide prevalent zoonotic parasite which causes toxoplasmosis. An appropriate vaccine for animals could interrupt the circle between animals and humans. Our previous study showed that excreted/secreted antigens (E/SA), derived from the peritoneum of mice infected with T. gondii tachyzoites could be considered as a good candidate for animal vaccination. Interleukin-10 (IL-10) inhibits proliferation of B and T lymphocytes and induces homeostasis in immune system responses. However, since IL-10 has also been shown to suppress the killing of T. gondii by human macrophages, the aim of this study was to evaluate IL-10 serum levels after vaccination with T. gondii E/SA prepared in vivo.
  
  
  METHODS
  T. gondii tachyzoites were inoculated in the peritoneum of mice and harvested E/SA were used as a vaccine, with and without adjuvant, in T. gondii infected and un-infected mice. IL-10 serum levels were evaluated using the ELISA technique.
  
  
  RESULTS
  The data showed that although serum levels of IL-10 were not changed at the early phases, they were elevated at the end phases of vaccination with T. gondii E/SA.
  
  
  CONCLUSIONS
  Based on these and our previous results, it can be concluded that in vivo prepared T. gondii E/SA could be considered as a good candidate for animal vaccination.
authors.affiliation: Molecular Medicine Research Center, Rafsanjan University of Medical Science, Rafsanjan, Iran.
authors.forename: Seyed Hossein
authors.initials: SH
authors.lastname: Abdollahi
authors.forename: Fateme
authors.initials: F
authors.lastname: Ayoobi
authors.forename: Hossein
authors.initials: H
authors.lastname: Khorramdelazad
authors.forename: Gholamhossein
authors.initials: G
authors.lastname: Hassanshahi
authors.forename: Behzad Nasiri
authors.initials: BN
authors.lastname: Ahmadabadi
authors.forename: Mohammadtaghi
authors.initials: M
authors.lastname: Rezayati
authors.forename: Ali
authors.initials: A
authors.lastname: Ravary
authors.forename: Ali
authors.initials: A
authors.lastname: Shamsizadeh
authors.forename: Mohammad Kazemi
authors.initials: MK
authors.lastname: Arababadi
keywords: Excreted/secreted antigens; IL-10; Toxoplasma gondii; Vaccination