title: Novel PCR-based methods enhance characterization of vaginal microbiota in a bacterial vaginosis patient before and after treatment.
abstract: Deep characterization, even by next-generation sequencing, of the vaginal microbiota in healthy women or posttreatment bacterial vaginosis patients is limited by the dominance of lactobacilli. To improve detection, we offer two approaches: quantitative PCR (qPCR) using phylogenetic branch-inclusive primers and sequencing of broad-spectrum amplicons generated with oligomers that block amplification of lactobacilli.
authors.affiliation: Department of Medicine, Division of Infectious Diseases, Wayne State University School of Medicine, Detroit, MI, USA.
authors.forename: Janet A
authors.initials: JA
authors.lastname: Lambert
authors.forename: Apoorv
authors.initials: A
authors.lastname: Kalra
authors.forename: Cristina T
authors.initials: CT
authors.lastname: Dodge
authors.forename: Susan
authors.initials: S
authors.lastname: John
authors.forename: Jack D
authors.initials: JD
authors.lastname: Sobel
authors.forename: Robert A
authors.initials: RA
authors.lastname: Akins
mesh_terms: D017931:DNA Primers / Q000235:genetics; D005260:Female; D014644:Genetic Variation; D006801:Humans; D007778:Lactobacillus / Q000235:genetics; D054892:Metagenome / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014621:Vagina / Q000382:microbiology*; D016585:Vaginosis, Bacterial / Q000188:drug therapy* / Q000382:microbiology*
chemical_list: D017931:DNA Primers