title: [Analysis on correlation between 3-hydroxy-3-methylglutary-coenzyme A reductase gene polymorphism of Glycyrrhiza uralensis and content of glycyrrhizic acid].
abstract: OBJECTIVE
  To reveal the 3-hydroxy-3-methylglutary-coenzyme A reductase (HMGR) gene polymorphism of Glycyrrhiza uralensis, and the correlation between HMGR gene polymorphism and the content of glycyrrhizic acid.
  
  
  METHODS
  Liquorice plants containing different content of glycyrrhizic acid were used as materials. RT-PCR was used to amplify their HMGR gene sequences, which were connected with vector pMD19-T for clone sequencing. Multiple alignments were performed to analyse HMGR gene polymorphism of G. uralensis. Then the correlation between HMGR gene polymorphism and the content of glycyrrhizic acid was revealed.
  
  
  RESULTS
  HMGR gene sequences polymorphism included codon mutation, base substitution mutation, copy number polymorphism and allele heterozygosity. There were 4 types of mutations in HMGR gene coding amino acid sequences, namely -HSL, -HSV, GALLV, GALSV. Among them, -HSV type was common in liquorice plants, -HSL type only existed in liquorice plants with low content of glycyrrhizic acid, and GALSV type only existed in liquorice plants with high content of glycyrrhizic acid.
  
  
  CONCLUSIONS
  HMGR gene sequences of G. uralensis are highly polymorphic and related to the content of glycyrrhizic acid.
authors.affiliation: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. liuyliwd@vip.sina.com
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Qiao-Xian
authors.initials: QX
authors.lastname: Xu
authors.forename: Xue-Yong
authors.initials: XY
authors.lastname: Wang
authors.forename: Chun-Sheng
authors.initials: CS
authors.lastname: Liu
authors.forename: Hong-Hao
authors.initials: HH
authors.lastname: Chen
mesh_terms: D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000737:chemistry / Q000145:classification / Q000235:genetics; D029882:Glycyrrhiza uralensis / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D019695:Glycyrrhizic Acid / Q000378:metabolism*; D006903:Hydroxymethylglutaryl CoA Reductases / Q000235:genetics* / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; D007527:Isoenzymes; D010940:Plant Proteins; D019695:Glycyrrhizic Acid; D006903:Hydroxymethylglutaryl CoA Reductases