title: Overexpression of MicroRNA-1 improves the efficacy of mesenchymal stem cell transplantation after myocardial infarction.
abstract: BACKGROUND
  The aim of this research was to study whether transplantation of mesenchymal stem cells (MSCs) overexpressing microRNA-1 into mouse infarcted myocardium can enhance cardiac myocyte differentiation and improve cardiac function efficiently.
  
  
  METHODS
  Eight-week-old female C57BL/6 mice underwent ligation of the left coronary artery to produce models of myocardial infarction. The ligated animals were randomly divided into 4 groups (20 in each). One week later, they were intramyocardially injected at the heart infarcted zone with microRNA-1-transduced MSCs (MSC(miR-1) group), mock-vector-transduced MSCs (MSC(null) group), MSCs (MSC group) or medium (PBS group). At 4 weeks post-transplantation, transthoracic echocardiographic assessment, histological evaluation and Western blot were performed.
  
  
  RESULTS
  The transplanted MSCs were able to differentiate into cardiomyocytes in the infarcted zone. Cardiac function in the MSC, MSC(null) and MSC(miR-1) groups was significantly improved compared to the PBS group (p < 0.01 or p < 0.001). However, treatment of MSCs expressing microRNA-1 was more effective for cardiac repair and improved cardiac function more efficiently by enhancing cell survival and cardiac myocyte differentiation compared to the MSC group or the MSC(null) groups (p < 0.05 or p < 0.01, respectively).
  
  
  CONCLUSIONS
  Transplantation of microRNA-1-transfected MSCs was more conducive to repair of infarct injury and improved heart function by enhancing transplanted cells survival and cardiomyogenic differentiation.
authors.affiliation: Department of Cardiology, Second Xiangya Hospital of Central South University, Changsha, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Huang
authors.forename: Mei-Ling
authors.initials: ML
authors.lastname: Li
authors.forename: Zhen-Fei
authors.initials: ZF
authors.lastname: Fang
authors.forename: Xin-Qun
authors.initials: XQ
authors.lastname: Hu
authors.forename: Qi-Ming
authors.initials: QM
authors.lastname: Liu
authors.forename: Zhen-Jiang
authors.initials: ZJ
authors.lastname: Liu
authors.forename: Liang
authors.initials: L
authors.lastname: Tang
authors.forename: Yan-Shu
authors.initials: YS
authors.lastname: Zhao
authors.forename: Sheng-Hua
authors.initials: SH
authors.lastname: Zhou
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D015687:Cell Hypoxia / Q000502:physiology; D003331:Coronary Vessels; D004195:Disease Models, Animal; D005260:Female; D006085:Graft Survival; D015552:Injections, Intralesional; D008026:Ligation; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000378:metabolism* / Q000494:pharmacology; D009200:Myocardial Contraction / Q000502:physiology; D009203:Myocardial Infarction / Q000628:therapy*; D010641:Phenotype; D011897:Random Allocation; D014162:Transfection
chemical_list: D015415:Biomarkers; D035683:MicroRNAs