title: Anti-oxidant and anti-inflammatory activity of leaf extracts and fractions of Mangifera indica.
abstract: OBJECTIVE
  To evaluate the anti-oxidant and anti-inflammatory activity of leaf extracts and fractions of Mangifera indica in in vitro conditions.
  
  
  METHODS
  In vitro DPPH radical scavenging activity and lipoxygenase (LOX) inhibition assays were used to evaluate the anti-oxidant and anti-inflammatory activities respectively. Methanolic extract (MEMI), successive water extract (SWMI) and ethyl acetate fraction (EMEMI), n-butanol fraction (BMEMI) and water soluble fraction (WMEMI) of methanolic extract were evaluated along with respective reference standards.
  
  
  RESULTS
  In in vitro DPPH radical scavenging activity, the MEMI, EMEMI and BMEMI have offered significant antioxidant activity with IC(50) values of 13.37, 3.55 and 14.19 μg/mL respectively. Gallic acid, a reference standard showed significant antioxidant activity with IC(50) value of 1.88 and found to be more potent compared to all the extracts and fractions. In in vitro LOX inhibition assay, the MEMI, EMEMI and BMEMI have showed significant inhibition of LOX enzyme activity with IC(50) values of 96.71, 63.21 and 107.44 μg/mL respectively. While, reference drug Indomethacin also offered significant inhibition against LOX enzyme activity with IC(50) of 57.75. Furthermore, MEMI was found to more potent than SWMI and among the fractions EMEMI was found to possess more potent antioxidant and anti-inflammatory activity.
  
  
  CONCLUSIONS
  These findings suggest that the MEMI and EMEMI possess potent anti-oxidant and anti-inflammatory activities in in vitro conditions.
authors.affiliation: Department of Pharmacognosy, Government College of Pharmacy, Bangalore-560 027, Karnataka, India. mohangcp@gmail.com
authors.forename: C G
authors.initials: CG
authors.lastname: Mohan
authors.forename: M
authors.initials: M
authors.lastname: Deepak
authors.forename: G L
authors.initials: GL
authors.lastname: Viswanatha
authors.forename: G
authors.initials: G
authors.lastname: Savinay
authors.forename: V
authors.initials: V
authors.lastname: Hanumantharaju
authors.forename: C E
authors.initials: CE
authors.lastname: Rajendra
authors.forename: Praveen D
authors.initials: PD
authors.lastname: Halemani
mesh_terms: D000893:Anti-Inflammatory Agents / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry*; D001713:Biphenyl Compounds / Q000737:chemistry; D008084:Lipoxygenase / Q000032:analysis; D016859:Lipoxygenase Inhibitors / Q000737:chemistry; D031022:Mangifera; D010851:Picrates / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D016859:Lipoxygenase Inhibitors; D010851:Picrates; D010936:Plant Extracts; C004931:1,1-diphenyl-2-picrylhydrazyl; D008084:Lipoxygenase