title: Identification of phenotypic markers of B cells from patients with Chagas disease.
abstract: Chagas disease was discovered more than a hundred years ago, but its pathogenesis is still not completely understood. Autoimmunity is one of the mechanisms shown to contribute to its pathogenesis, which may indicate an important participation of B lymphocytes. Patients with Chagas disease have shown increased percentage of B cells producing IL-10. However, there are no reports of the phenotypic markers of B cells producing IL-10 in patients with Chagas disease. For the first time in the literature, we evaluated the phenotypic profile of distinct markers of B cells from peripheral blood of noninfected individuals and patients with Chagas disease. Our results showed that patients with Chagas disease had a higher expression of CD21 and CD24 on the surface of CD19+ B cells, while CD43 and CD23 were expressed equally in all groups. Moreover, the expression of MHC-II (HLA-DR), CD80, CD86, caspase-3, granzyme B and intracellular IL-10 and TGF-β by CD19+ B cells was higher in patients with Chagas disease. The results of IL-10 production within CD19+ CD5+ CD1d+ B cells showed a higher percentage of this cytokine in patients with Chagas disease. Thus, our data bring a new knowledge about distinct markers of B cells in immune responses of Chagas disease.
authors.affiliation: René Rachou Research Center, FIOCRUZ, Belo Horizonte, MG, Brazil.
authors.forename: R C G
authors.initials: RC
authors.lastname: Fares
authors.forename: R
authors.initials: R
authors.lastname: Correa-Oliveira
authors.forename: F F
authors.initials: FF
authors.lastname: de Araújo
authors.forename: T S L
authors.initials: TS
authors.lastname: Keesen
authors.forename: A T
authors.initials: AT
authors.lastname: Chaves
authors.forename: J A
authors.initials: JA
authors.lastname: Fiuza
authors.forename: K S
authors.initials: KS
authors.lastname: Ferreira
authors.forename: M O C
authors.initials: MO
authors.lastname: Rocha
authors.forename: J A S
authors.initials: JA
authors.lastname: Gomes
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000032:analysis; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis; D053148:Caspase 3 / Q000378:metabolism; D014355:Chagas Disease / Q000276:immunology* / Q000378:metabolism; D005260:Female; D006684:HLA-DR Antigens / Q000276:immunology / Q000378:metabolism; D006801:Humans; D016130:Immunophenotyping; D016753:Interleukin-10 / Q000096:biosynthesis / Q000276:immunology; D008297:Male; D008875:Middle Aged; D016212:Transforming Growth Factor beta / Q000378:metabolism; D014349:Trypanosoma cruzi / Q000276:immunology
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; D006684:HLA-DR Antigens; D016212:Transforming Growth Factor beta; D016753:Interleukin-10; D053148:Caspase 3
keywords: B lymphocytes; Chagas disease; IL-10; immunoregulation; phenotypic markers