title: High frequency oscillatory ventilation versus conventional ventilation in a newborn piglet model with acute lung injury.
abstract: BACKGROUND
  High frequency oscillatory ventilation (HFOV) is considered a protective strategy for human lungs. This study was designed to define microscopic structural features of lung injury following HFOV with a high lung volume strategy in newborn piglets with acute lung injury.
  
  
  METHODS
  After acute lung injury with saline lavage, newborn piglets were randomly assigned to 5 study groups (6 in each group): control (no mechanical ventilation), conventional mechanical ventilation for 24 hours, conventional ventilation for 48 hours, HFOV for 24 hours, and HFOV for 48 hours. The right upper lung tissue was divided into the gravitation-dependent and gravitation-nondependent regions after the completion of mechanical ventilation. Under light microscopy, the numbers of polymorphonuclear leukocytes (PMNLs), alveolar macrophages, red blood cells, and hyaline membrane/alveolar edema were assessed in all lung tissues. Oxygenation index was continuously monitored.
  
  
  RESULTS
  Our results showed that the degree of histopathologic lung damage in the gravitation-dependent region was greater than that in the gravitation-nondependent region. Compared with the control group, PMNLs, red blood cells and hyaline membrane/alveolar edemas were significantly increased and alveolar macrophages were significantly decreased in lung tissues of conventional ventilation and HFOV piglets. In HFOV with high lung volume strategy piglets, lung tissues had significantly fewer PMNLs, red blood cells, and hyaline membrane/alveolar edemas, and oxygenation was improved significantly, compared to those of the conventional ventilation piglets.
  
  
  CONCLUSIONS
  Histopathologic lung damage in newborn piglets with lung injury was more severe in the gravitation-dependent region than in the gravitation-nondependent region. HFOV with high lung volume strategy reduced pulmonary PMNL infiltration, hemorrhage, alveolar edema, and hyaline membrane formation with improved oxygenation.
authors.affiliation: Department of Pediatrics, Guangdong Second Provincial People's Hospital, Guangzhou, China. wanhaifu@163.com
authors.forename: Wanhai
authors.initials: W
authors.lastname: Fu
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Qin
authors.forename: Chuming
authors.initials: C
authors.lastname: You
authors.forename: Qiong
authors.initials: Q
authors.lastname: Meng
authors.forename: Youwei
authors.initials: Y
authors.lastname: Zhao
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000473:pathology* / Q000628:therapy*; D000818:Animals; D000831:Animals, Newborn; D002452:Cell Count; D004195:Disease Models, Animal; D004487:Edema / Q000473:pathology*; D004912:Erythrocytes; D006112:Gravitation; D006819:Hyaline Membrane Disease / Q000473:pathology*; D016676:Macrophages, Alveolar; D009504:Neutrophils; D012121:Respiration, Artificial / Q000379:methods*; D012965:Sodium Chloride; D013552:Swine
chemical_list: D012965:Sodium Chloride