title: Physiological consequences of mutations in the htpG heat shock gene of Escherichia coli.
abstract: Mutation of the heat shock gene, htpG, causes severe defects of several cellular functions in Escherichia coli. A null htpG mutant constructed by gene replacement was impaired in the biosynthesis and secretion of several enzymes, and in biofilm formation and proteolysis. A significant decrease in the activity of β-lactamase in the ΔhtpG mutant was observed at 42°C. The alkaline phosphatase activity in sonicates of cells propagated at this raised temperature was lower in the ΔhtpG mutant than in the wild-type strain. The ability of the ΔhtpG mutant to degrade abnormal proteins was also impaired compared with the wild-type, but was increased at 42°C. Assays based on bioluminescence and crystal violet staining demonstrated that biofilm formation was diminished in the ΔhtpG mutant at the elevated temperature. All these defects can be complemented upon introducing htpG wild allele.
authors.affiliation: Department of Bacterial Genetics, Institute of Microbiology, University of Warsaw, Warsaw, Poland. grudam@biol.uw.edu.pl
authors.forename: Anna M
authors.initials: AM
authors.lastname: Grudniak
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Pawlak
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Bartosik
authors.forename: Krystyna I
authors.initials: KI
authors.lastname: Wolska
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D018441:Biofilms; D002172:Canavanine / Q000378:metabolism; D004926:Escherichia coli / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism*; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism*; D008163:Luminescent Measurements; D018832:Molecular Chaperones / Q000378:metabolism; D009154:Mutation; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D018841:HSP90 Heat-Shock Proteins; C489352:HtpG protein, E coli; D018832:Molecular Chaperones; D002172:Canavanine; D000469:Alkaline Phosphatase; D001618:beta-Lactamases