title: Studies on structural and physical characteristics of a novel exopolysaccharide from Pseudozyma sp. NII 08165.
abstract: The aim of this work was to study the production of exopolysaccharide (EPS) from a novel ustilaginomycetes yeast strain Pseudozyma sp. NII 08165. The culture produced 3.5g/l EPS on fourth day of fermentation in a glucose-based medium. The structural characterization revealed that the EPS was a polymer of glucose, galactose and mannose in the ratio of 2.4:5.0:2.6 with a molecular weight of 1.7MDa. The pseudoplastic behaviour of aqueous EPS with a thermal stability up to 220°C indicated its potential utility as a thickening or gelling agent in food industry. SEM studies of the EPS showed that it had compact film-like structure, which could make it a useful in preparing plasticized films. The AFM studies showed that EPS had spike-shaped microstructure. Physical properties of the exopolysaccharide determined further indicated its possible potential in different industrial applications.
authors.affiliation: Biotechnology Division, CSIR - National Institute for Interdisciplinary Science and Technology, Trivandrum 695019, India.
authors.forename: Kuttuvan Valappil
authors.initials: KV
authors.lastname: Sajna
authors.forename: Rajeev K
authors.initials: RK
authors.lastname: Sukumaran
authors.forename: Lalitha Devi
authors.initials: LD
authors.lastname: Gottumukkala
authors.forename: Himani
authors.initials: H
authors.lastname: Jayamurthy
authors.forename: Kiran S
authors.initials: KS
authors.lastname: Dhar
authors.forename: Ashok
authors.initials: A
authors.lastname: Pandey
mesh_terms: D003470:Culture Media; D005285:Fermentation; D062610:Fungal Polysaccharides / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D005690:Galactose / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D006358:Hot Temperature; D008358:Mannose / Q000737:chemistry; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D014587:Ustilaginales / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism
chemical_list: D003470:Culture Media; D062610:Fungal Polysaccharides; D005947:Glucose; D008358:Mannose; D005690:Galactose