title: A DOTAM-based paraCEST agent favoring TSAP geometry for enhanced amide proton chemical shift dispersion and temperature sensitivity.
abstract: The Tm(3+) chelate of DOTAM [1,4,7,10-tetrakis(carbamoylmethyl)-1,4,7,10-tetraazacyclododecane] possessing sterically demanding t-butyl amide substitution favors TSAP geometry. This chelate displayed a paraCEST signal associated with the highly shifted amide proton signal at approximately -100 ppm that was beyond the frequency of macromolecule magnetization transfer. This signal also displayed high temperature dependence (0.57 ppm °C(-1)) in the range of 35-42 °C and at neutral pH.
authors.affiliation: Centre for Functional and Metabolic Mapping, Robarts Research Institute, London, Ontario, Canada.
authors.forename: Todd K
authors.initials: TK
authors.lastname: Stevens
authors.forename: Mark
authors.initials: M
authors.lastname: Milne
authors.forename: Adam A H
authors.initials: AA
authors.lastname: Elmehriki
authors.forename: Mojmír
authors.initials: M
authors.lastname: Suchý
authors.forename: Robert
authors.initials: R
authors.lastname: Bartha
authors.forename: Robert H E
authors.initials: RH
authors.lastname: Hudson
mesh_terms: D000081:Acetamides / Q000737:chemistry*; D000577:Amides / Q000737:chemistry*; D003287:Contrast Media / Q000138:chemical synthesis*; D006573:Heterocyclic Compounds, 1-Ring / Q000737:chemistry*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D011522:Protons
chemical_list: C522450:1,4,7,10-tetrakis(carbamoylmethyl)-1,4,7,10-tetraazacyclododecane; D000081:Acetamides; D000577:Amides; D003287:Contrast Media; D006573:Heterocyclic Compounds, 1-Ring; D011522:Protons