title: A new quantitative method to determine the uptake of SPIONs in animal tissue and its application to determine the quantity of nanoparticles in the liver and lung of Balb-c mice exposed to the SPIONs.
abstract: We propose a new method for determining the quantity of superparamagnetic iron oxide nanoparticles (Fe3O4, SPIONs) embedded in animal tissue using magnetization measurements. With this method, the smallest detectable quantity of magnetite nanoparticles in a tissue sample is -1 microg. We showed that this method has proved being efficient. In this study, we focused in determining the quantity of SPION confined in lung and liver tissue of mice injected with -13 nm magnetite superparamagnetic nanoparticles. Furthermore, the method allowed us to detect the magnetite nanoparticles present in animal tissues without letting the natural iron ions present in the tissue or blood interfere with the measurements.
authors.affiliation: Centro Atómico Bariloche and Instituto Balseiro/CONICET (8400) S. C. de Bariloche, RN, Argentina.
authors.forename: R D
authors.initials: RD
authors.lastname: Zysler
authors.forename: E
authors.initials: E
authors.lastname: Lima
authors.forename: M
authors.initials: M
authors.lastname: Vasquez Mansilla
authors.forename: H E
authors.initials: HE
authors.lastname: Troiani
authors.forename: M L
authors.initials: ML
authors.lastname: Mojica Pisciotti
authors.forename: P
authors.initials: P
authors.lastname: Gurman
authors.forename: A
authors.initials: A
authors.lastname: Lamagna
authors.forename: L
authors.initials: L
authors.lastname: Colombo
mesh_terms: D000818:Animals; D003911:Dextrans / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008168:Lung / Q000378:metabolism*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D036103:Nanotechnology / Q000379:methods*; D009928:Organ Specificity
chemical_list: D003911:Dextrans; D058185:Magnetite Nanoparticles; C065507:ferumoxides