title: Special AT-rich sequence-binding protein 1 promotes cell growth and metastasis in colorectal cancer.
abstract: OBJECTIVE
  To evaluate the expression of special AT-rich sequence-binding protein 1 (SATB1) gene in colorectal cancer and its role in colorectal cancer cell proliferation and invasion.
  
  
  METHODS
  Immunohistochemistry was used to detect the protein expression of SATB1 in 30 colorectal cancer (CRC) tissue samples and pair-matched adjacent non-tumor samples. Cell growth was investigated after enhancing expression of SATB1. Wound-healing assay and Transwell assay were used to investigate the impact of SATB1 on migratory and invasive abilities of SW480 cells in vitro. Nude mice that received subcutaneous implantation or lateral tail vein were used to study the effects of SATB1 on tumor growth or metastasis in vivo.
  
  
  RESULTS
  SATB1 was over-expressed in CRC tissues and CRC cell lines. SATB1 promotes cell proliferation and cell cycle progression in CRC SW480 cells. SATB1 overexpression could promote cell growth in vivo. In addition, SATB1 could significantly raise the ability of cell migration and invasion in vitro and promote the ability of tumor metastasis in vivo. SATB1 could up-regulate matrix metalloproteases 2, 9, cyclin D1 and vimentin, meanwhile SATB1 could down-regulate E-cadherin in CRC.
  
  
  CONCLUSIONS
  SATB1 acts as a potential growth and metastasis promoter in CRC. SATB1 may be useful as a therapeutic target for CRC.
authors.affiliation: Department of Medical Oncology, Second Affiliated Hospital, Zhejiang University College of Medicine, Hangzhou 310000, Zhejiang Province, China.
authors.forename: Xue-Feng
authors.initials: XF
authors.lastname: Fang
authors.forename: Zhi-Bo
authors.initials: ZB
authors.lastname: Hou
authors.forename: Xin-Zheng
authors.initials: XZ
authors.lastname: Dai
authors.forename: Cong
authors.initials: C
authors.lastname: Chen
authors.forename: Jing
authors.initials: J
authors.lastname: Ge
authors.forename: Hong
authors.initials: H
authors.lastname: Shen
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Li
authors.forename: Li-Ke
authors.initials: LK
authors.lastname: Yu
authors.forename: Ying
authors.initials: Y
authors.lastname: Yuan
mesh_terms: D000818:Animals; D015703:Antigens, CD; D015820:Cadherins / Q000378:metabolism; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000378:metabolism*; D019938:Cyclin D1 / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D036961:Matrix Attachment Region Binding Proteins / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D015854:Up-Regulation; D014746:Vimentin / Q000378:metabolism; D014945:Wound Healing
chemical_list: D015703:Antigens, CD; C530104:CCND1 protein, human; C513384:CDH1 protein, human; D015820:Cadherins; D036961:Matrix Attachment Region Binding Proteins; C076189:SATB1 protein, human; D014746:Vimentin; D019938:Cyclin D1; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9
keywords: Colorectal cancer; Invasion; Migration; Proliferation; Special AT-rich sequence-binding protein 1