title: The function and properties of the transcriptional regulator COS1 in Magnaporthe oryzae.
abstract: The conidiophore stalk-less1 (COS1) gene encodes a novel transcription factor in Magnaporthe oryzae, and mutation of COS1 (M2942) resulted in developmental failure of conidiophores. COS1 putatively encodes a 491-amino-acid protein, which contains four multiple adjacent C2H2-type zinc-finger domains. The motifs are homologous to the zinc-finger protein Azf1 of Saccharomyces cerevisiae. Here, we report that the differences of expression profile between M2942 and the wild-type isolate Y34 by RNA-Sequence. DNA sequence analysis of promoter regions of those of COS1-dependent genes showed enrichment in the DNA sequence AAAAGAAA (A4GA3), the putative COS1-binding motif. Gel shift experiments showed that COS1 binds to DNA elements with A4GA3 motif. These suggest that many of the COS1-dependent transcripts may be regulated directly by COS1 binding.
authors.affiliation: State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Li
authors.forename: Xiuxiu
authors.initials: X
authors.lastname: Han
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Liu
authors.forename: Chaozu
authors.initials: C
authors.lastname: He
mesh_terms: D001665:Binding Sites; D004271:DNA, Fungal / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D055786:Gene Knockout Techniques; D020082:Magnaporthe / Q000166:cytology / Q000235:genetics* / Q000254:growth & development; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D013172:Spores, Fungal / Q000166:cytology / Q000254:growth & development; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D016335:Zinc Fingers
chemical_list: D004271:DNA, Fungal; D005656:Fungal Proteins; D014157:Transcription Factors