title: The 16S rRNA and mcrA gene based comparative diversity of methanogens in cattle fed on high fibre based diet.
abstract: In the present study, the diversity of rumen methanogens in crossbred Karan Fries cattle was determined by constructing 16S rRNA and mcrA (methyl coenzyme-M reductase α subunit) gene libraries using specific primers. All thirteen OTUs or phylotypes from 16S rRNA library clustered with order Methanobacteriales, twelve of which aligned with Methanobrevibacter spp., whereas one OTU resemble with Methanosphaera stadtmanae. Out of eighteen OTUs identified from mcrA gene library, fifteen clustered with order Methanobacteriales, two resemble with Methanomicrobiales and remaining one grouped with Methanosarcinales. These results revealed that Methanobrevibacter phylotype was predominantly present in Karan Fries crossbred cattle fed on high fibrous diet containing wheat straw. Compared to 16S rRNA gene, mcrA gene OTUs clustered in three orders providing better insights of rumen methanogens diversity in cattle.
authors.affiliation: Nutrition Biotechnology Lab, Dairy Cattle Nutrition Division, National Dairy Research Institute, Karnal-132001, Haryana, India. nutritionbiotechnology@gmail.com
authors.forename: Sunil Kumar
authors.initials: SK
authors.lastname: Sirohi
authors.forename: Prem Prashant
authors.initials: PP
authors.lastname: Chaudhary
authors.forename: Nasib
authors.initials: N
authors.lastname: Singh
authors.forename: Dheer
authors.initials: D
authors.lastname: Singh
authors.forename: Anil Kumar
authors.initials: AK
authors.lastname: Puniya
mesh_terms: D000818:Animals; D002417:Cattle; D004262:DNA Restriction Enzymes / Q000235:genetics*; D004032:Diet; D008297:Male; D017012:Methanobacteriales / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S; D012417:Rumen / Q000382:microbiology
chemical_list: D012336:RNA, Ribosomal, 16S; D004262:DNA Restriction Enzymes; C060629:endodeoxyribonuclease McrA