title: Detection of a novel bunyavirus associated with fever, thrombocytopenia and leukopenia syndrome in Henan Province, China, using real-time reverse transcription PCR.
abstract: A novel bunyavirus associated with fever, thrombocytopenia and leukopenia syndrome (FTLS) was discovered in Henan Province, China. Here, we report the development of an assay for this novel bunyavirus based on real-time reverse transcription PCR (RT-PCR). The assay exhibited high sensitivity and specificity without cross-reactivity towards 13 other viruses that cause similar symptoms. To evaluate the performance of this assay in detecting clinical samples, we analysed 261 serum samples from patients in Henan Province between 2007 and 2010. Of these samples, 91.95 % were bunyavirus positive. Compared with serological assays, the real-time PCR assay was much more sensitive in identifying infected patients 1 to 7 days after the onset of symptoms.
authors.affiliation: Center for Disease Control and Prevention of Henan Province, Zhengzhou, 450016, PR China.
authors.forename: Xueyong
authors.initials: X
authors.lastname: Huang
authors.affiliation: Beijing BGI-GBI Biotech Co., Ltd, Beijing, 101300, PR China.|State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Dongdajie Road 20, Beijing, 100071, PR China.
authors.forename: Licheng
authors.initials: L
authors.lastname: Liu
authors.affiliation: Center for Disease Control and Prevention of Henan Province, Zhengzhou, 450016, PR China.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Du
authors.affiliation: Center for Disease Control and Prevention of Henan Province, Zhengzhou, 450016, PR China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Ma
authors.affiliation: Center for Disease Control and Prevention of Henan Province, Zhengzhou, 450016, PR China.
authors.forename: Yujiao
authors.initials: Y
authors.lastname: Mu
authors.affiliation: Beijing BGI-GBI Biotech Co., Ltd, Beijing, 101300, PR China.
authors.lastname: Pengzhi Wang
authors.affiliation: Center for Disease Control and Prevention of Henan Province, Zhengzhou, 450016, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Ma
authors.affiliation: Center for Disease Control and Prevention of Henan Province, Zhengzhou, 450016, PR China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Tang
authors.affiliation: Center for Disease Control and Prevention of Henan Province, Zhengzhou, 450016, PR China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Wang
authors.affiliation: Center for Disease Control and Prevention of Henan Province, Zhengzhou, 450016, PR China.
authors.forename: Kai
authors.initials: K
authors.lastname: Kang
authors.affiliation: Center for Disease Control and Prevention of Xinyang City, Xinyang, 464000, PR China.
authors.forename: Shiqiang
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, 101300, PR China.
authors.forename: Weili
authors.initials: W
authors.lastname: Wu
authors.affiliation: Center for Disease Control and Prevention of Henan Province, Zhengzhou, 450016, PR China.
authors.forename: Haomin
authors.initials: H
authors.lastname: Chen
authors.affiliation: Center for Disease Control and Prevention of Henan Province, Zhengzhou, 450016, PR China.
authors.forename: Guohua
authors.initials: G
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Dongdajie Road 20, Beijing, 100071, PR China.
authors.forename: Yinhui
authors.initials: Y
authors.lastname: Yang
authors.lastname: Yongqiangj
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Dongdajie Road 20, Beijing, 100071, PR China.
authors.lastname: Jiang
authors.affiliation: Center for Disease Control and Prevention of Henan Province, Zhengzhou, 450016, PR China.
authors.forename: Bianli
authors.initials: B
authors.lastname: Xu
authors.affiliation: Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, 101300, PR China.|Beijing BGI-GBI Biotech Co., Ltd, Beijing, 101300, PR China.|State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Dongdajie Road 20, Beijing, 100071, PR China.
authors.forename: Weijun
authors.initials: W
authors.lastname: Chen
mesh_terms: D002043:Bunyaviridae / Q000235:genetics* / Q000302:isolation & purification*; D002044:Bunyaviridae Infections / Q000453:epidemiology / Q000473:pathology / Q000821:virology*; D002681:China / Q000453:epidemiology; D005334:Fever / Q000821:virology; D005455:Fluorescent Antibody Technique; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007970:Leukopenia / Q000821:virology; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013577:Syndrome; D013921:Thrombocytopenia / Q000821:virology
chemical_list: D007074:Immunoglobulin G