title: Ultrastructural changes in the air-blood barrier in mice after intratracheal instillations of Asian sand dust and gold nanoparticles.
abstract: The purpose of this study was to investigate a possible translocation pathway of intratracheally instilled gold nanoparticles after the induction of acute pulmonary injury by Asian sand dust. ICR mice were intratracheally instilled with 800μg Asian sand particles (CJ-2 particles) 24h before instillation of 50-nm gold nanoparticles. Lungs from mice treated with Asian sand particles and gold nanoparticles showed an acute focal inflammation with an increased expression of proinflammatory cytokines (IL-6 and TNF-α) and oxidative stress markers (Cu/Zn SOD and iNOS) in alveolar macrophages, type I alveolar epithelial cells, and endothelial cells at the alveolar walls. Electron microscopy revealed a destruction of the alveolar walls with an increased number of endocytic vesicles in the cytoplasm of both type I epithelial cells and endothelial cells; gold nanoparticles were demonstrated in these endocytic vesicles. These findings suggest that translocation of the exposed nanoparticles may be enhanced in the lung tissues with acute inflammatory changes.
authors.affiliation: Department of Veterinary Pathology, Tottori University, Minami 4-101, Koyama, Tottori-shi, Tottori 680-8553, Japan; The United Graduated School of Veterinary Science, Yamaguchi University, Yoshida 1667-1, Yamaguchi-shi, Yamaguchi 753-8515, Japan.
authors.forename: Kasem
authors.initials: K
authors.lastname: Rattanapinyopituk
authors.forename: Akinori
authors.initials: A
authors.lastname: Shimada
authors.forename: Takehito
authors.initials: T
authors.lastname: Morita
authors.forename: Masako
authors.initials: M
authors.lastname: Togawa
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Hasegawa
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Seko
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Inoue
authors.forename: Hirohisa
authors.initials: H
authors.lastname: Takano
mesh_terms: D000393:Air Pollutants / Q000633:toxicity; D000818:Animals; D001208:Asia; D015824:Blood-Air Barrier / Q000648:ultrastructure*; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D004391:Dust; D004730:Endothelium, Vascular / Q000648:ultrastructure; D006046:Gold / Q000633:toxicity*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000633:toxicity*; D012822:Silicon Dioxide / Q000633:toxicity*
chemical_list: D000393:Air Pollutants; D004391:Dust; D006046:Gold; D012822:Silicon Dioxide
keywords: Air–blood barrier; Cu/Zn SOD; IL-6; Kosa; Laminin; TNF-α; Translocation pathway; iNOS