title: Antibodies to cannabinoid type 1 receptor co-react with stomatin-like protein 2 in mouse brain mitochondria.
abstract: Anti-cannabinoid type 1 receptor (CB1 ) polyclonal antibodies are widely used to detect the presence of CB1 in a variety of brain cells and their organelles, including neuronal mitochondria. Surprisingly, we found that anti-CB1 sera, in parallel with CB1 , also recognize the mitochondrial protein stomatin-like protein 2. In addition, we show that the previously reported effect of synthetic cannabinoid WIN 55,212-2 on mitochondrial complex III respiration is not detectable in purified mitochondrial preparations. Thus, our study indicates that a direct relationship between endocannabinoid signaling and mitochondrial functions in the cerebral cortex seems unlikely, and that caution should be taken interpreting findings obtained using anti-CB1 antibodies.
authors.affiliation: Department of Neurobiology, Yale University School of Medicine and Kavli Institute for Neuroscience, New Haven, CT, USA. yury.morozov@yale.edu
authors.forename: Yury M
authors.initials: YM
authors.lastname: Morozov
authors.forename: Martin H
authors.initials: MH
authors.lastname: Dominguez
authors.forename: Luis
authors.initials: L
authors.lastname: Varela
authors.forename: Marya
authors.initials: M
authors.lastname: Shanabrough
authors.forename: Marco
authors.initials: M
authors.lastname: Koch
authors.forename: Tamas L
authors.initials: TL
authors.lastname: Horvath
authors.forename: Pasko
authors.initials: P
authors.lastname: Rakic
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000196:embryology / Q000276:immunology*; D001923:Brain Chemistry; D045744:Cell Line, Tumor; D003429:Cross Reactions; D005260:Female; D007106:Immune Sera / Q000276:immunology*; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000737:chemistry / Q000276:immunology*; D051379:Mice; D008928:Mitochondria / Q000648:ultrastructure; D024101:Mitochondrial Proteins / Q000032:analysis / Q000737:chemistry / Q000276:immunology*; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000276:immunology*; D043884:Receptor, Cannabinoid, CB1 / Q000032:analysis / Q000276:immunology*
chemical_list: D007106:Immune Sera; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D009419:Nerve Tissue Proteins; D043884:Receptor, Cannabinoid, CB1; C586438:stomatin-like protein 2, mouse
keywords: CB
  1; SLP-2; antibody specificity; marijuana; mitochondrial respiration; serology