title: A monoclonal antibody against a novel Sialomucin CD300LG.
abstract: CD300LG is a novel O-glycosylated member of the CD300 antigen-like family. Besides a classical mucin-like domain, it contains a V-type Ig domain. CD300LG binds lymphocyte L-selectin via its Ig domain and supports lymphocyte rolling via its mucin-like domain. The unique structure and function of CD300LG suggest it may play an important role in inflammation. For preparation of a monoclonal antibody (MAb) against human CD300LG, prokaryotic and eukaryotic expressing human CD300LG proteins were used as immunogen and detection antigen, respectively. One stable strain of hybridomas (3C7C5A6) was successfully established using the hybridoma technique. The Western blot and immunohistochemistry analyses demonstrated that the MAb was directed against human CD300LG with high specificity. This antibody could possibly facilitate studies on the pathomechanism of inflammation and may have the potential to be a means of effective anti-inflammation.
authors.affiliation: Department of Immunology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Jiang
authors.forename: Haibin
authors.initials: H
authors.lastname: Wang
authors.forename: Zhiguang
authors.initials: Z
authors.lastname: Li
authors.forename: Dapeng
authors.initials: D
authors.lastname: Wei
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Yang
authors.forename: Xulei
authors.initials: X
authors.lastname: Zheng
authors.forename: Jianhong
authors.initials: J
authors.lastname: Bi
authors.forename: Chongjie
authors.initials: C
authors.lastname: Zhang
mesh_terms: D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000096:biosynthesis / Q000737:chemistry*; D000918:Antibody Specificity; D015703:Antigens, CD / Q000096:biosynthesis / Q000276:immunology*; D015153:Blotting, Western; D016466:CHO Cells; D003412:Cricetulus; D004926:Escherichia coli; D005260:Female; D006801:Humans; D006825:Hybridomas; D007114:Immunization; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D018482:Muscle, Skeletal / Q000378:metabolism; D011971:Receptors, Immunologic / Q000096:biosynthesis / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000276:immunology
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D015703:Antigens, CD; C584286:CD300LG protein, human; D011971:Receptors, Immunologic; D011993:Recombinant Fusion Proteins