title: Poly(L-lactic acid)/hydroxyapatite nanocylinders as nanofibrous structure for bone tissue engineering scaffolds.
abstract: Poly (L-lactic acid) (PLLA), a biodegradable and biocompatible polyester, has been used as material of for tissue engineered scaffold and regenerative medicine. In this study, hydroxyapatite (HAp) and PLLA composite was electrospun. PLLA fibers were treated with an amino groups containing base in order to fabricate amino groups modified polymeric microcylinders. Simvastatin was loaded into the PLLA/HAp microcylinders. Our study focuses on the incorporation HAp and Simvastatin into the fibrous and cylindrical structure, its effects on the loading and release of simvastatin, and biological responses to osteoblast cells. The aspect ratio of microcylinders was tunable by varying the aminolysis treatment time and density of the base. The effects of the PLLA/HAp composites on osteoblast cell proliferation were evaluated. The PLLA/HAp may interact with osteoblast and obtain desired effects that were stimulating osteoblast function and restraining the osteoclast function for bone tissue regeneration. The potential of the developed composite microcylinders could be a promising way to fabricate the microfibrous structural scaffold that accelerates cell proliferation and functions for bone tissue engineering.
authors.affiliation: Department of Maxillofacial Biomedical Engineering, School of Dentistry, Kyung Hee University, Seoul, 130-701, Korea.
authors.forename: Jung Bok
authors.initials: JB
authors.lastname: Lee
authors.forename: Ha Na
authors.initials: HN
authors.lastname: Park
authors.forename: Wan-Kyu
authors.initials: WK
authors.lastname: Ko
authors.forename: Min Soo
authors.initials: MS
authors.lastname: Bae
authors.forename: Dong Nyoung
authors.initials: DN
authors.lastname: Heo
authors.forename: Dae Hyeok
authors.initials: DH
authors.lastname: Yang
authors.forename: Il Keun
authors.initials: IK
authors.lastname: Kwon
mesh_terms: D000588:Amines / Q000737:chemistry; D000818:Animals; D001842:Bone and Bones / Q000187:drug effects / Q000502:physiology*; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D017886:Durapatite / Q000494:pharmacology*; D019344:Lactic Acid / Q000494:pharmacology*; D051379:Mice; D041681:NIH 3T3 Cells; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D011091:Polyesters; D011108:Polymers / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000588:Amines; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D017886:Durapatite