title: Protein-assisted fabrication of nano-reduced graphene oxide for combined in vivo photoacoustic imaging and photothermal therapy.
abstract: Theranostic agents are attracting a great deal of attention in personalized medicine. Here, we developed a protein-based, facile method for fabrication of nanosized, reduced graphene oxide (nano-rGO) with high stability and low cytotoxicity. We constructed highly integrated photoacoustic/ultrasonic dual-modality imaging and photothermal therapy platforms, and further demonstrated that the prepared nano-rGO can be used as ready-to-use theranostic agents for both photoacoustic imaging and photothermal therapy without further surface modification. Intravenous administration of nano-rGO in tumor-bearing mice showed rapid and significant photoacoustic signal enhancement in the tumor region, indicating its excellence for passive targeting and photoacoustic imaging. Meanwhile, using a continuous-wave near-infrared laser, cancer cells in vivo were efficiently ablated, due to the photothermal effect of nano-rGO. The results suggest that the nano-rGO with protein-assisted fabrication was well suited for photoacoustic imaging and photothermal therapy of tumor, which is promising for theranostic nanomedicine.
authors.affiliation: Guangdong Key Laboratory of Nanomedicine, Shenzhen Key Laboratory of Cancer Nanotechnology, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
authors.forename: Zonghai
authors.initials: Z
authors.lastname: Sheng
authors.forename: Liang
authors.initials: L
authors.lastname: Song
authors.forename: Jiaxiang
authors.initials: J
authors.lastname: Zheng
authors.forename: Dehong
authors.initials: D
authors.lastname: Hu
authors.forename: Meng
authors.initials: M
authors.lastname: He
authors.forename: Mingbin
authors.initials: M
authors.lastname: Zheng
authors.forename: Guanhui
authors.initials: G
authors.lastname: Gao
authors.forename: Ping
authors.initials: P
authors.lastname: Gong
authors.forename: Pengfei
authors.initials: P
authors.lastname: Zhang
authors.forename: Yifan
authors.initials: Y
authors.lastname: Ma
authors.forename: Lintao
authors.initials: L
authors.lastname: Cai
mesh_terms: D000818:Animals; D002417:Cattle; D002470:Cell Survival; D005260:Female; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D006979:Hyperthermia, Induced / Q000379:methods*; D007259:Infrared Rays; D061986:MCF-7 Cells; D051379:Mice; D008819:Mice, Nude; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009928:Organ Specificity; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry*; D061088:Photoacoustic Techniques / Q000379:methods*; D056951:Photoelectron Spectroscopy; D010789:Phototherapy / Q000379:methods*; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet; D019265:Spectroscopy, Near-Infrared; D018675:Toxicity Tests; D014465:Ultrasonics; D023041:Xenograft Model Antitumor Assays
chemical_list: D010087:Oxides; D012710:Serum Albumin, Bovine; D006108:Graphite