title: Qualitative and quantitative mass spectrometry imaging of drugs and metabolites.
abstract: Mass spectrometric imaging (MSI) has rapidly increased its presence in the pharmaceutical sciences. While quantitative whole-body autoradiography and microautoradiography are the traditional techniques for molecular imaging of drug delivery and metabolism, MSI provides advantageous specificity that can distinguish the parent drug from metabolites and modified endogenous molecules. This review begins with the fundamentals of MSI sample preparation/ionization, and then moves on to both qualitative and quantitative applications with special emphasis on drug discovery and delivery. Cutting-edge investigations on sub-cellular imaging and endogenous signaling peptides are also highlighted, followed by perspectives on emerging technology and the path for MSI to become a routine analysis technique.
authors.affiliation: Department of Chemistry, University of Wisconsin-Madison, 777 Highland Avenue, Madison, WI 53705-2222, USA.
authors.forename: Christopher B
authors.initials: CB
authors.lastname: Lietz
authors.forename: Erin
authors.initials: E
authors.lastname: Gemperline
authors.forename: Lingjun
authors.initials: L
authors.lastname: Li
mesh_terms: D000818:Animals; D003952:Diagnostic Imaging / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000378:metabolism*
chemical_list: D004364:Pharmaceutical Preparations
keywords: ADME; Molecular imaging; PK/PD; Pharmaceuticals; Quantitative mass spectrometry; Spatial distribution