title: Differential transcription patterns in wild-type and glycoprotein G-deleted infectious laryngotracheitis viruses.
abstract: Infectious laryngotracheitis virus (ILTV) causes severe respiratory disease in poultry throughout the world. Recently the role of glycoprotein G (gG) in ILTV pathogenesis has been investigated and it has been shown to have chemokine-binding activity. An ILTV vaccine candidate deficient in gG has been developed and the deletion has been shown to alter the host's immune response to the virus. To understand the effect of the gG gene on transcription of other viral genes, the global expression profile of 72 ILTV genes in gG-deleted and wild-type ILTVs were investigated both in vivo and in vitro using quantitative reverse transcription-polymerase chain reaction. Several genes were differentially expressed in the different viruses in LMH cell cultures or in the tracheas of infected birds, and the expression of a number of genes, including ICP27, gC, gJ, Ul7 and UL40, differed significantly both in vivo and in vitro, suggesting that they had direct or indirect roles in virulence. This study has provided insights into the interactions between gG and other ILTV genes that may have a role in virulence.
authors.affiliation: Asia-Pacific Centre for Animal Health, The University of Melbourne, Melbourne, Victoria, Australia. alim@alumni.unimelb.edu.au
authors.forename: Alireza
authors.initials: A
authors.lastname: Mahmoudian
authors.forename: Philip F
authors.initials: PF
authors.lastname: Markham
authors.forename: Amir H
authors.initials: AH
authors.lastname: Noormohammadi
authors.forename: Joanne M
authors.initials: JM
authors.lastname: Devlin
authors.forename: Glenn F
authors.initials: GF
authors.lastname: Browning
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002645:Chickens; D017931:DNA Primers / Q000235:genetics; D020869:Gene Expression Profiling / Q000662:veterinary; D015967:Gene Expression Regulation, Viral / Q000235:genetics*; D006566:Herpesviridae Infections / Q000662:veterinary*; D001352:Herpesvirus 1, Gallid / Q000235:genetics* / Q000472:pathogenicity; D011201:Poultry Diseases / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D014759:Viral Envelope Proteins / Q000235:genetics*; D014765:Viral Vaccines / Q000235:genetics*; D014774:Virulence
chemical_list: D017931:DNA Primers; D014759:Viral Envelope Proteins; D014765:Viral Vaccines