title: Suppression of cellular apoptosis susceptibility (CSE1L) inhibits proliferation and induces apoptosis in colorectal cancer cells.
abstract: The cellular apoptosis susceptibility (CSE1L) gene has been demonstrated to regulate multiple cellular mechanisms including the mitotic spindle check point as well as proliferation and apoptosis. However, the importance of CSE1L in human colon cancer is largely unknown. In the present study, we examined expression levels of CSE1L mRNA by semiquantitative RT-PCR. A lentivirus-mediated small interfering RNA (siRNA) was used to knock down CSE1L expression in the human colon cancer cell line RKO. Changes in CSE1L target gene expression were determined by RT-PCR. Cell proliferation was examined by a high content screening assay. In vitro tumorigenesis was measured by colony-formation assay. Cell cycle distribution and apoptosis were detected by flow cytometric analysis. We found CSE1L mRNA to be expressed in human colon cancer cells. Using a lentivirus based RNAi approach, CSE1L expression was significantly inhibited in RKO cells, causing cell cycle arrest in the G2/M and S phases and a delay in cell proliferation, as well as induction of apoptosis and an inhibition of colony growth capacity. Collectively, the results suggest that silencing of CSE1L may be a potential therapeutic approach for colon cancer.
authors.affiliation: Department of General Surgery and Laparoscopic Center, Second Affiliated Hospital, Zhejiang University School of Medicine, 2Hangzhou, China.
authors.forename: Jin-Hui
authors.initials: JH
authors.lastname: Zhu
authors.forename: De-Fei
authors.initials: DF
authors.lastname: Hong
authors.forename: Yong-Mao
authors.initials: YM
authors.lastname: Song
authors.forename: Li-Feng
authors.initials: LF
authors.lastname: Sun
authors.forename: Zhi-Fei
authors.initials: ZF
authors.lastname: Wang
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival / Q000235:genetics; D029563:Cellular Apoptosis Susceptibility Protein / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D006801:Humans; D059566:M Phase Cell Cycle Checkpoints / Q000235:genetics; D034622:RNA Interference; D012333:RNA, Messenger / Q000096:biosynthesis; D034741:RNA, Small Interfering; D059807:S Phase Cell Cycle Checkpoints / Q000235:genetics
chemical_list: D029563:Cellular Apoptosis Susceptibility Protein; D012333:RNA, Messenger; D034741:RNA, Small Interfering