title: In non-small cell lung cancer mitogenic signaling leaves Sprouty1 protein levels unaffected.
abstract: Sprouty1 protein belongs to a family of receptor tyrosine kinase-mediated signaling inhibitors, whose members are usually regulated by growth factors to form a negative feedback loop. Correspondingly fluctuations of Sprouty1 mRNA in response to single growth factors have been observed. In this report, we investigate Sprouty1 protein levels and show that in non-small cell lung carcinoma-derived cells, the expression levels are unaffected by the serum content in the cellular environment. Although cells harboring K-Ras mutations express insignificant higher Sprouty1 levels, ectopic expression of constitutive active Ras in normal human lung fibroblasts fails to augment Sprouty1 protein content. Furthermore, serum starvation for three days has no influence on Sprouty1 expression and addition of serum or of singular growth factors leaves Sprouty protein levels unchanged. Cell cycle analysis reveals that Sprouty1 levels remain constant throughout the whole cell cycle. These data demonstrate that Sprouty1 expression is not connected with mitogenic signaling and cell proliferation.
authors.affiliation: Institute of Cancer Research, Department of Medicine I, Comprehensive Cancer Center, Medical University of Vienna, Vienna, Austria.
authors.forename: Rosana Maria
authors.initials: RM
authors.lastname: Kral
authors.forename: Christoph-Erik
authors.initials: CE
authors.lastname: Mayer
authors.forename: Vanita
authors.initials: V
authors.lastname: Vanas
authors.forename: Andrea
authors.initials: A
authors.lastname: Gsur
authors.forename: Hedwig
authors.initials: H
authors.lastname: Sutterlüty-Fall
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism* / Q000473:pathology; D002453:Cell Cycle; D045744:Cell Line, Tumor; D016895:Culture Media, Serum-Free; D004815:Epidermal Growth Factor / Q000494:pharmacology; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D051525:Fibroblast Growth Factor 9 / Q000494:pharmacology; D011905:Genes, ras; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D008565:Membrane Proteins / Q000235:genetics*; D008938:Mitosis; D009154:Mutation; D010750:Phosphoproteins / Q000235:genetics*; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D016895:Culture Media, Serum-Free; D051525:Fibroblast Growth Factor 9; D008565:Membrane Proteins; D010750:Phosphoproteins; C486055:SPRY1 protein, human; D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor; D020794:Receptor Protein-Tyrosine Kinases
keywords: NSCLC; Sprouty; Spry1; cell cycle