title: A comparative study of the influence of three pure titanium plates with different micro- and nanotopographic surfaces on preosteoblast behaviors.
abstract: There is a great demand for dental implants with the ability to accelerate periimplant bone regeneration. Modification of surface micro- and nanotopographies has been revealed to affect bone cell metabolism. In this study, we utilized dielectric barrier discharge (DBD) technology to modify commercially pure titanium (Ti-tr) surfaces and then investigated the cytocompability of DBD-modified Ti surface when compared with machined (Ti-m) and polished (Ti-p) Ti surfaces. These three kinds of Ti plates exhibited different surface energies and topographies at the micro- and nanoscale levels. The DBD-treated pure Ti surface significantly enhances cell adhesion, spread, and proliferation of MC3T3-E1 preosteoblast cells compared with the Ti-p and Ti-m surfaces, suggesting that Ti-tr has better cytocompatibility compared with the other two surfaces. Preosteoblast cells on Ti-m surface exhibited higher alkaline phosphatase activity than cells on Ti-tr and Ti-p surfaces 14 days after seeding. No significant difference in alkaline phosphatase activity was observed between cells grown on Ti-tr and Ti-p surfaces. Our study demonstrated that DBD modification significantly enhanced cell adhesion, spread, and proliferation of preosteoblasts with no negative effects on cell differentiation. Microtopography and nanotopography of the surfaces of different materials and chemical/energetic properties have a synergistic effect on cell attachment, proliferation, and differentiation.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Xiangya Hospital School of Stomatology, Central South University, Changsha, 410078, China; Shanghai Key Laboratory of Stomatology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zuo
authors.forename: Xunzhi
authors.initials: X
authors.lastname: Huang
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Zhong
authors.forename: Bangshang
authors.initials: B
authors.lastname: Zhu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Sun
authors.forename: Chengyu
authors.initials: C
authors.lastname: Jin
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Quan
authors.forename: Zhangui
authors.initials: Z
authors.lastname: Tang
authors.forename: Wantao
authors.initials: W
authors.lastname: Chen
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D022001:Focal Adhesions / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D018625:Microscopy, Atomic Force; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry* / Q000494:pharmacology*; D014867:Water / Q000737:chemistry
chemical_list: D014867:Water; D014025:Titanium; D000469:Alkaline Phosphatase
keywords: dielectric barrier discharges; oral implant; osteoblast; surface topography; titanium