title: A chemically sulfated polysaccharide from Grifola frondos induces HepG2 cell apoptosis by notch1-NF-κB pathway.
abstract: Sulfated polysaccharides have been known to inhibit proliferation in tumor cells. However, the molecular mechanisms involved in sulfated polysaccharides-induced apoptosis are still uncharacterized. In this study, the effect of a chemically sulfated polysaccharide obtained from Grifola frondosa (S-GFB) on HepG2 cell proliferation and apoptosis-related mechanism were investigated. It was found that S-GFB inhibited proliferation of HepG2 cells in a dose-dependent manner with IC50 at 48 h of 61 μg ml(-1). The results of scanning electron micrographs indicated that S-GFB induced typical apoptotic morphological feature in HepG2 cells. Flow cytometric analysis demonstrated that S-GFB caused apoptosis of HepG2 cells through cells arrested at S phase. Western-blotting results showed that S-GFB inhibited notch1 expression, IκB-α degradation and NF-κB/p65 translocation from cytoplasm into nucleus. Simultaneously, the apoptotic mechanism of HepG2 cells induced by S-GFB was associated with down regulation of FLIP, and activation of caspase-3 and caspase-8. Taken together, these findings suggest that the S-GFB induces apoptosis through a notch1/NF-κB/p65-mediated caspase pathway.
authors.affiliation: Key Laboratory of Food Nutrition and Safety (Tianjin University of Science & Technology), Ministry of Education, Tianjin, China. wangchunling@tust.edu.cn
authors.forename: Chun-ling
authors.initials: CL
authors.lastname: Wang
authors.forename: Meng
authors.initials: M
authors.lastname: Meng
authors.forename: Sheng-bin
authors.initials: SB
authors.lastname: Liu
authors.forename: Li-rui
authors.initials: LR
authors.lastname: Wang
authors.forename: Li-hua
authors.initials: LH
authors.lastname: Hou
authors.forename: Xiao-hong
authors.initials: XH
authors.lastname: Cao
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D053446:CASP8 and FADD-Like Apoptosis Regulating Protein / Q000378:metabolism; D053148:Caspase 3 / Q000378:metabolism; D053181:Caspase 8 / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D048691:Grifola; D056945:Hep G2 Cells; D006801:Humans; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D051881:Receptor, Notch1 / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D051996:Transcription Factor RelA / Q000378:metabolism*
chemical_list: D053446:CASP8 and FADD-Like Apoptosis Regulating Protein; C497761:NOTCH1 protein, human; D011134:Polysaccharides; D051881:Receptor, Notch1; D051996:Transcription Factor RelA; D053148:Caspase 3; D053181:Caspase 8