title: Resistance to the quorum-quenching compounds brominated furanone C-30 and 5-fluorouracil in Pseudomonas aeruginosa clinical isolates.
abstract: The quorum-quenching compounds brominated furanone C-30 and 5-fluorouracil inhibit the pathogenicity of the Pseudomonas aeruginosa laboratory strains PA01 and PA14; however, there is no report studying the effectiveness of these compounds for clinical isolates. Therefore, the effect of both quorum quenchers on the production of pyocyanin, elastase and alkaline protease of eight clinical strains from children was evaluated. Although both compounds were in general effective for the attenuation of these factors, three strains resistant to C-30 were found. For 5-fluorouracil, PA01 and some clinical isolates showed resistance for at least one phenotype.
authors.affiliation: Departamento de Bioquímica, Instituto Nacional de Cardiología, México, México. garrod13420@cardiologia.org.mx
authors.forename: Rodolfo
authors.initials: R
authors.lastname: García-Contreras
authors.forename: Mariano
authors.initials: M
authors.lastname: Martínez-Vázquez
authors.forename: Norma
authors.initials: N
authors.lastname: Velázquez Guadarrama
authors.forename: Alejandra Guadalupe
authors.initials: AG
authors.lastname: Villegas Pañeda
authors.forename: Takahiro
authors.initials: T
authors.lastname: Hashimoto
authors.forename: Toshinari
authors.initials: T
authors.lastname: Maeda
authors.forename: Héctor
authors.initials: H
authors.lastname: Quezada
authors.forename: Thomas K
authors.initials: TK
authors.lastname: Wood
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000032:analysis / Q000378:metabolism; D002648:Child; D024881:Drug Resistance, Bacterial; D010450:Endopeptidases / Q000032:analysis / Q000378:metabolism; D005472:Fluorouracil / Q000494:pharmacology*; D005663:Furans / Q000494:pharmacology*; D006801:Humans; D010196:Pancreatic Elastase / Q000032:analysis / Q000378:metabolism; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000302:isolation & purification / Q000378:metabolism; D011710:Pyocyanine / Q000032:analysis / Q000378:metabolism; D053038:Quorum Sensing / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D005663:Furans; C494198:furanone C-30; D011710:Pyocyanine; D010450:Endopeptidases; D010196:Pancreatic Elastase; C087822:alkaline protease; D005472:Fluorouracil