title: Monitoring of residues of ochratoxin A in blood and kidney of chicken using HPLC-FLD.
abstract: Detoxification of ochratoxin A can be achieved by chemical or enzymatic hydrolyzation, the products of such reactions are ochratoxin α and phenylalanine. Ochratoxin α like ochratoxin A, is a fluorescing molecule, therefore sensitive analysis is possible at very low concentration levels. Methods have been established that make it possible to look for residues of ochratoxin A and its main metabolite ochratoxin α in blood and tissues at very low concentration levels. Plasma is extracted by the use of small amounts of chloroform; the extract is cleaned with water and afterwards evaporated to dryness]. The residue is re-dissolved and analysed by HPLC-FLD. Using this method a limit of detection of 0.5μg/l for both ochratoxin A and ochratoxin α can be reached.
authors.affiliation: IFA-Tulln, Center for Analytical Chemistry, Konrad Lorenz Str. 20, A-3430, Tulln.
authors.forename: E
authors.initials: E
authors.lastname: Fuchs
authors.forename: E
authors.initials: E
authors.lastname: Binder
authors.forename: G
authors.initials: G
authors.lastname: Schatzmayr
authors.forename: D
authors.initials: D
authors.lastname: Heidler
authors.forename: A
authors.initials: A
authors.lastname: Klimitsch
authors.forename: R
authors.initials: R
authors.lastname: Krska