title: The non-coding RNA llme23 drives the malignant property of human melanoma cells.
abstract: Several lines of evidence support the notion that increased RNA-binding ability of polypyrimidine tract-binding (PTB) protein-associated splicing factor (PSF) and aberrant expression of long non-coding RNAs (lncRNAs) are associated with mouse and human tumors. To identify the PSF-binding lncRNA involved in human oncogenesis, we screened a nuclear RNA repertoire of human melanoma cell line, YUSAC, through RNA-SELEX affinity chromatography. A previously unreported lncRNA, termed as Llme23, was found to bind immobilized PSF resin. The specific binding of Llme23 to both recombinant and native PSF protein was confirmed in vitro and in vivo. The expression of PSF-binding Llme23 is exclusively detected in human melanoma lines. Knocking down Llme23 remarkably suppressed the malignant property of YUSAC cells, accompanied by the repressed expression of proto-oncogene Rab23. These results may indicate that Llme23 can function as an oncogenic RNA and directly associate the PSF-binding lncRNA with human melanoma.
authors.affiliation: Center for Functional Genomics and Bioinformatics, Ministry of Education, Key Laboratory for Bio-Resource and Eco-Environment, College of Life Science, Sichuan University, Chengdu 610064, China.
authors.forename: Chuan-Fang
authors.initials: CF
authors.lastname: Wu
authors.forename: Guang-Hong
authors.initials: GH
authors.lastname: Tan
authors.forename: Cheng-Chuan
authors.initials: CC
authors.lastname: Ma
authors.forename: Ling
authors.initials: L
authors.lastname: Li
mesh_terms: D015153:Blotting, Western; D063646:Carcinogenesis / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic; D045325:HCT116 Cells; D057809:HEK293 Cells; D056945:Hep G2 Cells; D006801:Humans; D061986:MCF-7 Cells; D008545:Melanoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D000072670:PTB-Associated Splicing Factor; D011485:Protein Binding; D000090063:Proto-Oncogene Mas; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014183:Transplantation, Heterologous; D020691:rab GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: C000715470:MAS1 protein, human; D000072670:PTB-Associated Splicing Factor; D000090063:Proto-Oncogene Mas; D062085:RNA, Long Noncoding; D016601:RNA-Binding Proteins; C486614:RAB23 protein, human; D020691:rab GTP-Binding Proteins