title: Epigallocatechin-3-gallate promotes apoptosis and expression of the caspase 9a splice variant in PC3 prostate cancer cells.
abstract: Growing evidence suggests that the flavonoid epigallocatechin-3-gallate (EGCG), notably abundant in green tea, has health-promoting properties. We examined the effect of EGCG on cell survival and apoptosis in the prostate cancer cell line PC3. Cell survival was reduced and apoptosis increased significantly with a low dose of 1 µM EGCG. The ability of the anticancer drug cisplatin to promote apoptosis was enhanced by EGCG. Furthermore, EGCG, both alone and in combination with cisplatin, promoted the expression of the pro-apoptotic splice isoform of caspase 9.
authors.affiliation: Centre for Research in Bioscience, Faculty of Health and Life Sciences, University of the West of England, Coldharbour Lane, Frenchay, Bristol BS16 1QY, UK.
authors.forename: Rachel M
authors.initials: RM
authors.lastname: Hagen
authors.forename: Veronica S
authors.initials: VS
authors.lastname: Chedea
authors.forename: Christopher P
authors.initials: CP
authors.lastname: Mintoff
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Bowler
authors.forename: H Ruth
authors.initials: HR
authors.lastname: Morse
authors.forename: Michael R
authors.initials: MR
authors.lastname: Ladomery
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D053453:Caspase 9 / Q000096:biosynthesis / Q000235:genetics*; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D020033:Protein Isoforms / Q000235:genetics
chemical_list: D020033:Protein Isoforms; D002392:Catechin; C045651:epigallocatechin gallate; D053453:Caspase 9; D002945:Cisplatin