title: Manipulating magnetic 3D spheroids in hanging drops for applications in tissue engineering and drug screening.
abstract: Magnetic spheroid manipulation can be carried out in hanging drops to generate distinctly structured heterotypic microtissues through sequential addition of cells or spheroid to homotypic spheroids. These spheroids can also be incorporated in a droplet-based assay to screen for therapeutic efficacy in prolonged studies. This simple and versatile technique can offer potential benefits in tissue engineering and drug screening applications.
authors.affiliation: Molecular Engineering Laboratory, A*STAR, Proteos #03-13, 61 Biopolis Drive, Singapore 138673, Singapore. hohbv@bmsi.a-star.edu.sg.
authors.forename: Vincent H B
authors.initials: VH
authors.lastname: Ho
authors.forename: Wei Mei
authors.initials: WM
authors.lastname: Guo
authors.forename: Charlotte L
authors.initials: CL
authors.lastname: Huang
authors.forename: Shu Fen
authors.initials: SF
authors.lastname: Ho
authors.forename: Su Yin
authors.initials: SY
authors.lastname: Chaw
authors.forename: Ern Yu
authors.initials: EY
authors.lastname: Tan
authors.forename: Kee Woei
authors.initials: KW
authors.lastname: Ng
authors.forename: Joachim S C
authors.initials: JS
authors.lastname: Loo
mesh_terms: D004353:Drug Evaluation, Preclinical / Q000379:methods*; D057809:HEK293 Cells; D006801:Humans; D060328:Magnetic Phenomena; D018874:Spheroids, Cellular / Q000166:cytology*; D023822:Tissue Engineering / Q000379:methods*; D014407:Tumor Cells, Cultured
keywords: drug screening; magnetic separation; multicellular spheroids; nanoparticles; tissue engineering