title: A dual enzyme microgel with high antioxidant ability based on engineered seleno-ferritin and artificial superoxide dismutase.
abstract: An antioxidant microgel with both glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities is reported. Using computational design and genetic engineering methods, the main catalytic components of GPx are fabricated onto the surface of ferritin. The resulting seleno-ferritin (Se-Fn) monomers can self-assemble into nanocomposites that exhibit remarkable GPx activity due to the well organized multi-GPx catalytic centers. Subsequently, a porphyrin derivative is synthesized as an SOD mimic, and is employed to construct a synergistic dual enzyme system by crosslinking Se-Fn nanocomposites into a microgel. Significantly, this dual enzyme microgel is demonstrated to display better antioxidant ability than single GPx or SOD mimics in protecting cells from oxidative damage.
authors.affiliation: State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, PR China.
authors.forename: Yuzhou
authors.initials: Y
authors.lastname: Gao
authors.forename: Chunxi
authors.initials: C
authors.lastname: Hou
authors.forename: Lipeng
authors.initials: L
authors.lastname: Zhou
authors.forename: Dongmei
authors.initials: D
authors.lastname: Zhang
authors.forename: Chunqiu
authors.initials: C
authors.lastname: Zhang
authors.forename: Lu
authors.initials: L
authors.lastname: Miao
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.forename: Zeyuan
authors.initials: Z
authors.lastname: Dong
authors.forename: Quan
authors.initials: Q
authors.lastname: Luo
authors.forename: Junqiu
authors.initials: J
authors.lastname: Liu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D055162:Biocatalysis; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel; D005293:Ferritins / Q000378:metabolism*; D005782:Gels / Q000737:chemistry*; D005979:Glutathione Peroxidase / Q000378:metabolism; D015227:Lipid Peroxidation; D008933:Mitochondrial Swelling; D008958:Models, Molecular; D011166:Porphyrins / Q000138:chemical synthesis / Q000737:chemistry; D015202:Protein Engineering; D012643:Selenium / Q000378:metabolism*; D013057:Spectrum Analysis; D013482:Superoxide Dismutase / Q000378:metabolism*; D013997:Time Factors
chemical_list: D000975:Antioxidants; D005782:Gels; D011166:Porphyrins; C051232:5,10,15,20-tetra(4-hydroxyphenyl)porphyrin; D005293:Ferritins; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D012643:Selenium