title: Structural and functional based identification of the bean (Phaseolus) microRNAs and their targets from expressed sequence tags.
abstract: MicroRNAs (miRNAs) are small, 18-26 nucleotides long, non-coding RNAs that play role in post-transcriptional gene regulation. Many of these are evolutionarily conserved. This suggests a powerful approach to predict new miRNAs in other species. In this research, structural and functional approaches were combined to make computational prediction of potential miRNAs and their targets in Bean (Phaseolus). Total 55 novel miRNAs were detected from 38 miRNAs families in Bean (Phaseolus). These families are; miR156, 160, 164, 168, 170, 171, 172, 319, 393, 396, 397, 398, 408, 414, 438, 444, 535, 1310, 1424, 1426, 1848, 1860, 1863, 2055, 2091, 2093, 2094, 2102, 2103, 2105, 2864, 2866, 2925, 2926, 4221, 4245, 4246 and 4250. In the 55 putative miRNAs; 28 miRNAs belong to Phaseolus acutifolius, 23 to Phaseolus vulgaris, 4 to Phaseolus coccineus. All the mature miRNAs reside in the stem portion of the hairpin structures. Total 146 potential protein targets were predicted for these miRNAs.
authors.affiliation: Department of Botany, University of Balochistan, Sariab Road, Quetta, Pakistan. barozaikhan@gmail.com
authors.forename: Muhammad Younas Khan
authors.initials: MY
authors.lastname: Barozai
authors.forename: Muhammad
authors.initials: M
authors.lastname: Din
authors.forename: Iftikhar Ahmed
authors.initials: IA
authors.lastname: Baloch
mesh_terms: D001483:Base Sequence; D017124:Conserved Sequence; D020224:Expressed Sequence Tags; D018506:Gene Expression Regulation, Plant; D035683:MicroRNAs / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D027805:Phaseolus / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D018749:RNA, Plant; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D035683:MicroRNAs; D018749:RNA, Plant; D016601:RNA-Binding Proteins