title: Production and characterization of polyclonal and monoclonal antibodies against Aflatoxin B1 oxime-BSA in an enzyme-linked immunosorbent assay.
abstract: From a single aflatoxin B1 oxime - bovine serum albumin conjugate, polyclonal and monoclonal antibody preparations were produced. The four rabbit polyclonal antisera were specific for aflatoxin Bi in a microtitration plate enzyme - linked immunosorbent assay. The monoclonal antibodies showed a wide range of differing specificities, recognizing, for example, aflatoxins B1, B2, G1 and G2; B1 and B2; B1 and G1; and G1 alone. No antibody preparations reacted with aflatoxin M1. The significance of these results to the strategy of anti-aflatoxin antibody production for use in quantitative enzyme immunoassays is discussed.
authors.affiliation: AFRC Institute of Food Research, Colney Lane, NR4 7UA, Norwich, UK.
authors.forename: C
authors.initials: C
authors.lastname: Ward
authors.forename: A
authors.initials: A
authors.lastname: Wilkinson
authors.forename: S
authors.initials: S
authors.lastname: Bramham
authors.forename: H
authors.initials: H
authors.lastname: Lee
authors.forename: H S
authors.initials: HS
authors.lastname: Chan
authors.forename: G
authors.initials: G
authors.lastname: Butcher
authors.forename: A
authors.initials: A
authors.lastname: Hutchings
authors.forename: M
authors.initials: M
authors.lastname: Morgan