title: [Association of HLA-G 14bp gene polymorphisms and plasma sHLA-G level with susceptibility to EV71 infection in children].
abstract: OBJECTIVE
  Explore the relationship between the HLA-G 14bp insertion/deletion polymorphism and the infection of Enterovirus 71 (EV71) for children.
  
  
  METHODS
  We genotyped HLA-G 14bp insertion/deletion polymorphism of 125 severe HFMD children infected with EV71 and 133 normal controls by PCR-PAGE;detected the plasma sHLA-G level of 66 heavy type and 15 critical type and 133 normal controls by ELISA.
  
  
  RESULTS
  Frequencies of the genotype 14 bp - / - ,14 bp + / - and 14 bp + / + were 49.6% , 42.4% and 8.0% for the severe HFMD children infected with EV71, and 34.6%, 48.9% and 16.5% for the normal controls, respectively. A significant difference was observed for the frequencies of the HLA-G 14bp genotype between the two groups(chi2 = 7.850, P = 0.020). And for the allele frequencies. The plasma sHLA-G levels in heavy type were dramatically higher than that in normal controls (Z = -9.692, P = 0.000). The plasma sHLA-G levels in children with critical HFMD were dramatically higher than that with heavy type (Z = -2.420, P = 0.016).
  
  
  CONCLUSIONS
  There was a relationship between the HLA-G 14 bp insertion/deletion polymorphism and the susceptibility to the severe HFMD children infected with EV71 and the plasma sHLA-G might be considered as a index for auxiliary diagnosis the severe HFMD infected with EV71.
authors.affiliation: The Second Affiliated Hospital of Wenzhou Medical College, Wenzhou, China.
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Chen
authors.forename: Hui-Yan
authors.initials: HY
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Gao
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: Yu
authors.forename: Dong
authors.initials: D
authors.lastname: Chen
authors.forename: Xiao-Qun
authors.initials: XQ
authors.lastname: Zheng
mesh_terms: D002648:Child; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D004198:Disease Susceptibility; D029821:Enterovirus A, Human / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D004769:Enterovirus Infections / Q000097:blood / Q000453:epidemiology / Q000235:genetics* / Q000821:virology*; D005260:Female; D059951:HLA-G Antigens / Q000097:blood* / Q000235:genetics*; D006801:Humans; D007223:Infant; D008297:Male; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D011110:Polymorphism, Genetic; D017384:Sequence Deletion
chemical_list: D059951:HLA-G Antigens