title: Monoclonal antibody against membrane protein of transmissible gastroenteritis virus.
abstract: Transmissible gastroenteritis virus (TGEV) is a porcine coronavirus that can cause piglet diarrhea with high mortality rates. TGEV membrane (M) protein not only plays a vital role in the process of virus assembly and budding, but also induces the production of interferon-α during infection. In this study, a monoclonal antibody (MAb) designated 7G7, against the TGEV M protein was generated by inoculating BALB/c mice with TGEV followed by hybridoma technique. Immunofluorescence assays indicated that MAb 7G7 was capable of detecting cell infection by TGEV. Virus-based ELISA demonstrated that MAb 7G7 can be used as a highly specific diagnostic reagent for TGEV.
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Xiangfang District, Harbin, China.
authors.forename: Xuejiao
authors.initials: X
authors.lastname: Sun
authors.forename: Yudong
authors.initials: Y
authors.lastname: Ren
authors.forename: Yu
authors.initials: Y
authors.lastname: Li
authors.forename: Jiayi
authors.initials: J
authors.lastname: Zhu
authors.forename: Weijuan
authors.initials: W
authors.lastname: Zhu
authors.forename: Fan
authors.initials: F
authors.lastname: Ding
authors.forename: Guangxing
authors.initials: G
authors.lastname: Li
authors.forename: Chunfeng
authors.initials: C
authors.lastname: Wang
authors.forename: Ming
authors.initials: M
authors.lastname: Gao
authors.forename: Yunhang
authors.initials: Y
authors.lastname: Gao
authors.forename: Liyan
authors.initials: L
authors.lastname: Cao
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Ren
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D000918:Antibody Specificity; D000086862:Coronavirus M Proteins; D017931:DNA Primers / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D019084:Fluorescent Antibody Technique, Indirect; D005761:Gastroenteritis, Transmissible, of Swine / Q000175:diagnosis* / Q000821:virology*; D006825:Hybridomas / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D013552:Swine; D014763:Viral Matrix Proteins / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000086862:Coronavirus M Proteins; D017931:DNA Primers; C000706238:M protein, Transmissible gastroenteritis virus; D014763:Viral Matrix Proteins