title: Simple and efficient cell disruption of extremely small quantities of mycelium of phytopathogenic mycotoxin-producing moulds for quantitative extraction of genomic DNA.
abstract: DNA was efficiently and quantitatively isolated from extremely small quantities of mycelia (0.1-10 mg) of different phytopathogenic moulds by grinding freeze-dried mycelia with glass beads and then using a commercial DNA extraction kit. The efficiency of disruption of the mycelia and the quantitative DNA extraction was proved by microscopy and the quantification of isolated DNA by real time PCR.
authors.affiliation: Institut für Ernährungs- und Lebensmittelwissenschaften, Lebensmittel-Mikrobiologie und Hygiene, Rheinische Friedrich-Wilhelms-Universität Bonn, Meckenheimer Allee 168, 53115, Bonn, Germany.
authors.forename: V
authors.initials: V
authors.lastname: Saß
authors.forename: J
authors.initials: J
authors.lastname: Milles
authors.forename: J
authors.initials: J
authors.lastname: Krämer
authors.forename: A
authors.initials: A
authors.lastname: Prange