title: Preparation and evaluation of lysozyme-loaded nanoparticles coated with poly-γ-glutamic acid and chitosan.
abstract: To improve the application of lysozymes, methods for coating lysozymes with poly-γ-glutamic acid and chitosan were studied. Several lysozyme-loaded chitosan/poly-γ-glutamic acid composite nanosystems for loading and controlling the release of lysozymes were established. The lysozyme loading content and efficiency of the different systems were examined. The antibacterial activity of the composite nanoparticles was also investigated. Results showed that when the lysozymes were coated with poly-γ-glutamic acid and further rewrapped with chitosan, smooth spherical composite nanoparticles were obtained; the loading efficiency and loading content reached 76% and 40%, respectively. The lysozyme release in vitro was slow and presented a two-stage programmed release. Antibacterial testing in vitro indicated that lysozyme-loaded nanoparticles coated with poly-γ-glutamic acid/chitosan had outstanding antibacterial activity. An obvious assembly of bacterial cells and composite nanoparticles was observed during co-incubation. Therefore, the poly-γ-glutamic acid/chitosan composite coating broadened the antibacterial spectrum of the composite lysozyme nanoreagent, and presented satisfactory antibacterial effect. The lysozyme-loaded chitosan/poly-γ-glutamic acid nanocoating system established in this research could provide reference for coating and controlled releasing of alkaline proteins.
authors.affiliation: Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao Road, Hangzhou 310021, Zhejiang Province, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.forename: Yaoxing
authors.initials: Y
authors.lastname: Xu
authors.forename: Hai
authors.initials: H
authors.lastname: Feng
authors.forename: Sida
authors.initials: S
authors.lastname: Fu
authors.forename: Jiangwu
authors.initials: J
authors.lastname: Tang
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Dongchang
authors.initials: D
authors.lastname: Sun
authors.forename: Hua
authors.initials: H
authors.lastname: Jiang
authors.forename: Shaochun
authors.initials: S
authors.lastname: Xu
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects / Q000254:growth & development; D003692:Delayed-Action Preparations / Q000737:chemistry / Q000494:pharmacology*; D004339:Drug Compounding; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D009113:Muramidase / Q000737:chemistry / Q000494:pharmacology*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011099:Polyglutamic Acid / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development
chemical_list: D000900:Anti-Bacterial Agents; D003692:Delayed-Action Preparations; D011099:Polyglutamic Acid; D009113:Muramidase