title: Assessment the levels of tartrate-resistant acid phosphatase (TRAP) on mice fed with eggshell calcium citrate malate.
abstract: Optimized conditions were obtained by one-factor-at-a-time test (OFAT) and ternary quadratic regression orthogonal composite design (TQROCD) respectively. By pulse electric fields (PEF) technology, the process of eggshell calcium citrate malate (ESCCM), eggshell calcium citrate (ESCC) and eggshells calcium malate (ESCM) were comprehensive compared. The levels of tartrate-resistant acid phosphatase (TRAP) and the bioavailability on mice fed with eggshell calcium citrate malate (ESCCM) treated by pulsed electric field (PEF) were evaluated. Results showed that the rates of calcium dissolution of the different acids studied can be arranged as ESCCM (7.90 mg/mL)>ESCC (7.12 mg/mL)>ESCM (7.08 mg/mL) from highest to lowest rate of dissolution. At the same dose 133.0 mg kg(-1) d(-1), the levels of TRAP in the ESCCM treatment groups were significantly lower than those in ESCM and ESCC (P<0.05). Bone calcium content in the mice fed with ESCCM was generally higher than fed with ESCM and ESCC.
authors.affiliation: Laboratory of Nutrition and Functional Food, Jilin University, Changchun 130062, PR China.
authors.forename: Yiding
authors.initials: Y
authors.lastname: Yu
authors.forename: Mingdi
authors.initials: M
authors.lastname: Zhang
authors.forename: Songyi
authors.initials: S
authors.lastname: Lin
authors.forename: Liyan
authors.initials: L
authors.lastname: Wang
authors.forename: Jingbo
authors.initials: J
authors.lastname: Liu
authors.forename: Gregory
authors.initials: G
authors.lastname: Jones
authors.forename: Hsiang-Chi
authors.initials: HC
authors.lastname: Huang
mesh_terms: D000042:Absorption; D000135:Acid Phosphatase / Q000378:metabolism*; D000818:Animals; D001682:Biological Availability; D002118:Calcium / Q000008:administration & dosage* / Q000302:isolation & purification / Q000493:pharmacokinetics; D019343:Citric Acid / Q000008:administration & dosage* / Q000302:isolation & purification / Q000493:pharmacokinetics; D004531:Eggs; D004572:Electrolysis; D005269:Femur / Q000378:metabolism; D007527:Isoenzymes / Q000378:metabolism*; D008293:Malates / Q000008:administration & dosage* / Q000302:isolation & purification / Q000493:pharmacokinetics; D008297:Male; D051379:Mice; D010010:Osteoclasts / Q000201:enzymology; D000071681:Tartrate-Resistant Acid Phosphatase
chemical_list: D007527:Isoenzymes; D008293:Malates; D019343:Citric Acid; C083976:calcium citrate malate; D000135:Acid Phosphatase; C000605511:Acp5 protein, mouse; D000071681:Tartrate-Resistant Acid Phosphatase; D002118:Calcium