title: Phosphorylation regulates coilin activity and RNA association.
abstract: The Cajal body (CB) is a domain of concentrated components found within the nucleus of cells in an array of species that is functionally important for the biogenesis of telomerase and small nuclear ribonucleoproteins. The CB is a dynamic structure whose number and size change during the cell cycle and is associated with other nuclear structures and gene loci. Coilin, also known as the marker protein for the CB, is a phosphoprotein widely accepted for its role in maintaining CB integrity. Recent studies have been done to further elucidate functional activities of coilin apart from its structural role in the CB in an attempt to explore the rationale for coilin expression in cells that have few CBs or lack them altogether. Here we show that the RNA association profile of coilin changes in mitosis with respect to that during interphase. We provide evidence of transcriptional and/or processing dysregulation of several CB-related RNA transcripts as a result of ectopic expression of both wild-type and phosphomutant coilin proteins. We also show apparent changes in transcription and/or processing of these transcripts upon coilin knockdown in both transformed and primary cell lines. Additionally, we provide evidence of specific coilin RNase activity regulation, on both U2 and hTR transcripts, by phosphorylation of a single residue, serine 489. Collectively, these results point to additional functions for coilin that are regulated by phosphorylation.
authors.affiliation: Department of Biochemistry, The University of Mississippi Medical Center , Jackson, MS 39216-4505 , USA.
authors.forename: Hanna J
authors.initials: HJ
authors.lastname: Broome
authors.forename: Zunamys I
authors.initials: ZI
authors.lastname: Carrero
authors.forename: Heather E
authors.initials: HE
authors.lastname: Douglas
authors.forename: Michael D
authors.initials: MD
authors.lastname: Hebert
keywords: Cajal body; Telomerase; snRNA