title: Specific detection by real-time reverse-transcription PCR assays of a novel avian influenza A(H7N9) strain associated with human spillover infections in China.
abstract: In response to a recent outbreak in China, detection assays for a novel avian influenza A(H7N9) virus need to be implemented in a large number of public health laboratories. Here we present real-time reverse-transcription polymerase chain reaction (RT-PCR) assays for specific detection of this virus, along with clinical validation data and biologically-safe positive controls.
authors.affiliation: Institute of Virology, University of Bonn Medical Centre, Bonn, Germany.
authors.forename: V M
authors.initials: VM
authors.lastname: Corman
authors.forename: M
authors.initials: M
authors.lastname: Eickmann
authors.forename: O
authors.initials: O
authors.lastname: Landt
authors.forename: T
authors.initials: T
authors.lastname: Bleicker
authors.forename: S
authors.initials: S
authors.lastname: Brünink
authors.forename: M
authors.initials: M
authors.lastname: Eschbach-Bludau
authors.forename: M
authors.initials: M
authors.lastname: Matrosovich
authors.forename: S
authors.initials: S
authors.lastname: Becker
authors.forename: C
authors.initials: C
authors.lastname: Drosten
mesh_terms: D000818:Animals; D001717:Birds / Q000821:virology; D002681:China; D006801:Humans; D009980:Influenza A virus / Q000235:genetics* / Q000302:isolation & purification; D005585:Influenza in Birds / Q000635:transmission / Q000821:virology*; D007251:Influenza, Human / Q000175:diagnosis / Q000821:virology*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*