title: Magnetic nanoparticles-based extraction and verification of nucleic acids from different sources.
abstract: In many molecule biology and genetic technology studies, the amount of available DNA can be one of the important criteria for selecting the samples from different sources. Compared with those genomic DNA methods using organic solvents or other traditional commercial kits, the method based on magnetic nanoparticles (MNPs) and adsorption technology has many remarkable advantages like being time-saving and cost effective without the laborious centrifugation or precipitation steps, and more importantly it has the great potential and especially suitable for automated DNA extraction and up-scaling. In this paper, the extraction efficiency of genomic nucleic acids based on magnetic nanoparticles from four different sources including bacteria, yeast, human blood and virus samples are compared and verified. After measurement and verification of the extracted genomic nucleic acids, it was shown that all these genomic nucleic acids extracted using the MNPs method can be of high yield and be available for next molecule biological steps.
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Nanjing 210096, P R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: Ma
authors.forename: Chuanyan
authors.initials: C
authors.lastname: Li
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Ningning
authors.initials: N
authors.lastname: Ma
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Li
authors.forename: Zhiyang
authors.initials: Z
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Deng
authors.forename: Zhifei
authors.initials: Z
authors.lastname: Wang
authors.forename: Zhijiang
authors.initials: Z
authors.lastname: Xi
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Tang
authors.forename: Nongyue
authors.initials: N
authors.lastname: Hel
mesh_terms: D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004587:Electrophoresis, Agar Gel; D016678:Genome; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009696:Nucleic Acids / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D015183:Restriction Mapping; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D058185:Magnetite Nanoparticles; D009696:Nucleic Acids; D012822:Silicon Dioxide; D004247:DNA