title: Down-regulation of GRIM-19 is associated with STAT3 overexpression in breast carcinomas.
abstract: To evaluate the association of gene associated with retinoic-interferon-induced mortality 19 (GRIM-19) with clinicopathologic features as well as its target gene signal transducer and activator of transcription 3 (STAT3) in patients with breast cancer, GRIM-19 and STAT3 expression was measured immunohistochemically in 108 breast samples and by Western blotting in 20 breast cancer tissues and corresponding nontumorous tissues. Expression of GRIM-19 was severely depressed in the carcinomas relative to matched nontumorous tissues (P < .001), and STAT3 was overexpressed in breast cancer tissues (P < .001), conclusions supported by Western blot analysis. Nonexpression of GRIM-19 was significantly associated with lymph node metastasis (P < .001), advanced tumor-node-metastasis stage (P = .02), and triple-negative phenotype (P = .03). Furthermore, down-regulation of GRIM-19 correlated with STAT3 overexpression (r = 0.56; P < .001). Thus, GRIM-19 is suppressed in primary breast carcinomas, with a corresponding increase in STAT3 activity.
authors.affiliation: Department of Breast Surgery, Qilu Hospital of Shandong University, Jinan 250012, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhou
authors.forename: Lan
authors.initials: L
authors.lastname: Chao
authors.forename: Guohua
authors.initials: G
authors.lastname: Rong
authors.forename: Chenggang
authors.initials: C
authors.lastname: Wang
authors.forename: Rong
authors.initials: R
authors.lastname: Ma
authors.forename: Xiao
authors.initials: X
authors.lastname: Wang
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D000328:Adult; D000368:Aged; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism; D015153:Blotting, Western; D001940:Breast / Q000378:metabolism / Q000473:pathology; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D015536:Down-Regulation; D005260:Female; D006801:Humans; D007124:Immunoenzyme Techniques; D008198:Lymph Nodes / Q000473:pathology; D008207:Lymphatic Metastasis; D008408:Mastectomy; D008875:Middle Aged; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism*; D009367:Neoplasm Staging; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism; D011960:Receptors, Estrogen / Q000378:metabolism; D011980:Receptors, Progesterone / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000378:metabolism*
chemical_list: D051017:Apoptosis Regulatory Proteins; D014408:Biomarkers, Tumor; D009247:NADH, NADPH Oxidoreductases; D018719:Erb-b2 Receptor Tyrosine Kinases; D011960:Receptors, Estrogen; D011980:Receptors, Progesterone; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; C439057:NDUFA13 protein, human; C508053:ERBB2 protein, human
keywords: Breast cancer; GRIM-19; Immunohistochemistry; STAT3; Western blot