title: Yarrowia lipolytica dehydrogenase/reductase: an enzyme tolerant for lipophilic compounds and carbohydrate substrates.
abstract: Yarrowia lipolytica short chain dehydrogenase/reductase (YlSDR) was expressed in Escherichia coli, purified and characterized in vitro. The substrate scope for YlSDR mediated oxidation was investigated with alcohols and unprotected carbohydrates spectrophotometrically, revealing a preference for secondary compared to primary alcohols. In reduction direction, YlSDR was highly active on ribulose and fructose, suggesting that the enzyme is a mannitol-2-dehydrogenase. In order to explore substrate tolerance especially for space-demanding, lipophilic protecting groups, 5-O-trityl-D-ribitol and 5-O-trityl-α,β-D-ribose were investigated as substrates: YlSDR oxidized 5-O-trityl-D-ribitol and 5-O-trityl-α,β-D-ribose and reduced the latter at the expense of NADP(H).
authors.affiliation: Acib GmbH, Petersgasse 14, Graz 8010, Austria.
authors.forename: Kamila
authors.initials: K
authors.lastname: Napora
authors.forename: Tanja M
authors.initials: TM
authors.lastname: Wrodnigg
authors.forename: Patrick
authors.initials: P
authors.lastname: Kosmus
authors.forename: Martin
authors.initials: M
authors.lastname: Thonhofer
authors.forename: Karen
authors.initials: K
authors.lastname: Robins
authors.forename: Margit
authors.initials: M
authors.lastname: Winkler
mesh_terms: D004926:Escherichia coli / Q000378:metabolism; D064429:Fatty Acid Synthases / Q000235:genetics / Q000378:metabolism*; D005632:Fructose / Q000378:metabolism; D007700:Kinetics; D008354:Mannitol Dehydrogenases / Q000235:genetics / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D010429:Pentoses / Q000378:metabolism*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D012255:Ribitol / Q000378:metabolism*; D012266:Ribose / Q000378:metabolism; D013379:Substrate Specificity; D025062:Yarrowia / Q000201:enzymology*
chemical_list: D010429:Pentoses; D011994:Recombinant Proteins; D005632:Fructose; D012255:Ribitol; D009249:NADP; C100182:ribulose; D012266:Ribose; D008354:Mannitol Dehydrogenases; C036543:short chain trans-2-enoyl-CoA reductase; D009247:NADH, NADPH Oxidoreductases; D064429:Fatty Acid Synthases