title: Application of surface plasmon resonance spectroscopy for adsorption studies of different types of components on poly(dimethylsiloxane).
abstract: Surface plasmon resonance (SPR) spectroscopy is utilized to study in real-time and, by label-free means, the reversible and quasi-irreversible adsorption of small ionic or neutral molecules, pharmaceuticals, and proteins on poly(dimethylsiloxane) (PDMS) surfaces. The SPR sensor is covered with 0.2% (w/v) PDMS in octane. During the timescale of a typical lab-on-a-chip analysis or an electrophoretic separation, it was found that small neutral components containing a hydrophobic part do not adsorb or absorb onto PDMS, while larger, water-soluble polymer-like materials like proteins generally irreversibly adsorb to PDMS. The technique can be used to monitor the kinetics of adsorption and desorption of the molecules. For the non-specific adsorption of teicoplanin to PDMS, a Langmuir-like adsorption isotherm was obtained (K(d)=32±2 μmol L(-1)).
authors.affiliation: Department of Chemistry and Biochemistry, California State University, 5151 State University Drive, Los Angeles, CA 90032 8202, USA.
authors.forename: Attila
authors.initials: A
authors.lastname: Gaspar
authors.forename: Frank A
authors.initials: FA
authors.lastname: Gomez