title: Real-time monitoring in vitro cellular cytotoxicity of silica nanotubes using electric cell-substrate impedance sensing (ECIS).
abstract: An electrical measurement known as Electric Cell-substrate Impedance Sensing (ECIS) has become increasingly applied to the study of cellular viability, proliferation and cytotoxicity with the advantages of label-free, non-invasion and real-time monitoring capability in comparison with other conventional methods (MTS, MTT). With this technique, cells are grown on the micro-sized gold electrodes where the micro-ampere alternative current is applied to measure the impedance changes due to the physiological changes caused by internal or external stimuli. In another field, Silica Nanotubes (SNTs) are a novel class of inorganic structures with promising potentials in bio-separation, drug delivery, imaging and other biomedical applications. In this study, by using ECIS-based self-fabricated cell chip, Cells were cultured on the working electrodes and separately exposure to the 0, 2 microm, 2 microm and 10 microm long at the varying concentrations of SNTs to evaluate the cellular responses such as viability, multiplication time and cytotoxicity. Final results were additionally compared with the MTS method as a reference to review the reliability
authors.affiliation: School of Integrative Engineering, Department of Biomedical Engineering, Chung-Ang University, Heuseok-dong, Dongjak-gu, Seoul 156-756, Republic of Korea.
authors.forename: Trong Binh
authors.initials: TB
authors.lastname: Tran
authors.forename: Phuong Diem
authors.initials: PD
authors.lastname: Nguyen
authors.forename: Soong Ho
authors.initials: SH
authors.lastname: Um
authors.forename: Sang Jun
authors.initials: SJ
authors.lastname: Son
authors.forename: Junhong
authors.initials: J
authors.lastname: Min
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D003199:Computer Systems; D017097:Electric Impedance; D006801:Humans; D043942:Nanotubes / Q000633:toxicity* / Q000648:ultrastructure; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000633:toxicity*
chemical_list: D012822:Silicon Dioxide