title: Performance improvement of the one-dot lateral flow immunoassay for aflatoxin B1 by using a smartphone-based reading system.
abstract: This study was conducted to develop a simple, rapid, and accurate lateral flow immunoassay (LFIA) detection method for point-of-care diagnosis. The one-dot LFIA for aflatoxin B1 (AFB1) was based on the modified competitive binding format using competition between AFB1 and colloidal gold-AFB1-BSA conjugate for antibody binding sites in the test zone. A Smartphone-based reading system consisting of a Samsung Galaxy S2 Smartphone, a LFIA reader, and a Smartphone application for the image acquisition and data analysis. The detection limit of one-dot LFIA for AFB1 is 5 μg/kg. This method provided semi-quantitative analysis of AFB1 samples in the range of 5 to 1,000 μg/kg. Using combination of the one-dot LFIA and the Smartphone-based reading system, it is possible to conduct a more fast and accurate point-of-care diagnosis.
authors.affiliation: Post-Harvest & Food Engineering Division, Department of Agricultural Engineering, National Academy of Agricultural Sciences, Suwon 441707, Korea. sdlee96@gmail.com
authors.forename: Sangdae
authors.initials: S
authors.lastname: Lee
authors.forename: Giyoung
authors.initials: G
authors.lastname: Kim
authors.forename: Jihea
authors.initials: J
authors.lastname: Moon
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis*; D040421:Cell Phone / Q000295:instrumentation*; D003429:Cross Reactions; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods*; D057230:Limit of Detection; D012212:Rheology / Q000295:instrumentation*
chemical_list: D016604:Aflatoxin B1