title: Two-step method for encapsulation of oregano essential oil in chitosan nanoparticles: preparation, characterization and in vitro release study.
abstract: In this study, oregano essential oil (OEO) has been encapsulated in chitosan nanoparticles by a two-step method, i.e., oil-in-water emulsion and ionic gelation of chitosan with sodium tripolyphosphate (TPP). The success of OEO encapsulation was confirmed by Fourier transform infrared (FT-IR) spectroscopy, UV-vis spectrophotometry, thermogravimetric analysis (TGA) and X-ray diffraction (XRD) techniques. The obtained nanoparticles exhibited a regular distribution and spherical shape with size range of 40-80 nm as observed by scanning electron microscopy (SEM) and atomic force microscopy (AFM). As determined by TGA technique, the encapsulation efficiency (EE) and loading capacity (LC) of OEO-loaded chitosan nanoparticles were about 21-47% and 3-8%, respectively, when the initial OEO content was 0.1-0.8 g/g chitosan. In vitro release studies showed an initial burst effect and followed by a slow drug release.
authors.affiliation: Department of Fisheries, Faculty of Marine Sciences, Tarbiat Modares University, P.O. Box 46414-356, Noor, Iran.
authors.forename: Seyed Fakhreddin
authors.initials: SF
authors.lastname: Hosseini
authors.forename: Mojgan
authors.initials: M
authors.lastname: Zandi
authors.forename: Masoud
authors.initials: M
authors.lastname: Rezaei
authors.forename: Farhid
authors.initials: F
authors.lastname: Farahmandghavi
mesh_terms: D048271:Chitosan / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D009822:Oils, Volatile / Q000737:chemistry*; D031341:Origanum; D010316:Particle Size; D011122:Polyphosphates / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry; D014961:X-Ray Diffraction
chemical_list: D009822:Oils, Volatile; D011122:Polyphosphates; D048271:Chitosan; C005692:triphosphoric acid