title: Capillary electrophoretic and extraction conditions for the analysis of Catha edulis FORKS active principles.
abstract: A capillary electrophoretic method, which allowed the detection and separation of the active principles of Catha edulis, i.e. cathinone, cathine and phenylpropanolamine, was developed. A suitable internal standard (nicotinamide), which permitted the quantification of the analytes reducing the variability of the migration times due to EOF changes, was identified. The analytical method was validated, assessing linearity, sensitivity and repeatability, showing optimal features for the analysis of the vegetable material. Moreover extraction conditions were investigated to achieve the exhaustion of the plant material in the fastest and most efficient way to meet the requirements of the Court.
authors.affiliation: Dipartimento di Scienze Farmaceutiche, Università degli Studi di Milano, Via Mangiagalli 25, 20133 Milano, Italy. gabriella.roda@unimi.it
authors.forename: Gabriella
authors.initials: G
authors.lastname: Roda
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Liberti
authors.forename: Sebastiano
authors.initials: S
authors.lastname: Arnoldi
authors.forename: Antonella
authors.initials: A
authors.lastname: Argo
authors.forename: Chiara
authors.initials: C
authors.lastname: Rusconi
authors.forename: Sonia
authors.initials: S
authors.lastname: Suardi
authors.forename: Veniero
authors.initials: V
authors.lastname: Gambaro
mesh_terms: D000470:Alkaloids / Q000032:analysis / Q000737:chemistry; D029754:Catha / Q000737:chemistry*; D019075:Electrophoresis, Capillary; D015394:Molecular Structure; D010665:Phenylpropanolamine / Q000032:analysis / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D015203:Reproducibility of Results
chemical_list: D000470:Alkaloids; D010936:Plant Extracts; C005331:norpseudoephedrine; D010665:Phenylpropanolamine; C023665:cathinone