title: Crosslinked gelatin nanofibres: preparation, characterisation and in vitro studies using glial-like cells.
abstract: Gelatin (GL) nanofibrous matrices mimicking the complex biological structure of the natural extracellular matrix (ECM) were prepared from aqueous solutions by electrospinning technique. GL nanofibres with a diameter size of around 300nm were obtained optimising the process and solution parameters. To increase the GL stability in aqueous environment γ-glycidoxypropyltrimethoxysilane (GPTMS) was used as GL crosslinker. GPTMS crosslinking did not modify the nanofibrous matrix morphology: fibre diameter and membrane pores size were 327±45 nm and 1.64±0.37 μm, respectively. The produced GPTMS crosslinked GL nanofibres (GL/GPTMS_NF) were found to support the in vitro adhesion, proliferation and survival of neonatal olfactory bulb ensheating cells (NOBECs).
authors.affiliation: Politecnico di Torino, Department of Mechanical and Aerospace Engineering, Politecnico di Torino, Corso Duca degli Abruzzi 24, Torino, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Tonda-Turo
authors.forename: E
authors.initials: E
authors.lastname: Cipriani
authors.forename: S
authors.initials: S
authors.lastname: Gnavi
authors.forename: V
authors.initials: V
authors.lastname: Chiono
authors.forename: C
authors.initials: C
authors.lastname: Mattu
authors.forename: P
authors.initials: P
authors.lastname: Gentile
authors.forename: I
authors.initials: I
authors.lastname: Perroteau
authors.forename: M
authors.initials: M
authors.lastname: Zanetti
authors.forename: G
authors.initials: G
authors.lastname: Ciardelli
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D003432:Cross-Linking Reagents / Q000737:chemistry; D005109:Extracellular Matrix / Q000378:metabolism; D005780:Gelatin / Q000737:chemistry* / Q000378:metabolism; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D012995:Solubility; D054457:Tissue Scaffolds
chemical_list: D003432:Cross-Linking Reagents; D005780:Gelatin