title: Mitochondrial injury induced by nanosized titanium dioxide in A549 cells and rats.
abstract: The nanosized titanium dioxide (nano-TiO2) is an important nanoscale compound applied in many different fields because of its superior performance. Here, an anatase nano-TiO2 showed cytotoxicity in a dosage-dependent manner, which was in accordance with changes of A549 cell ultrastructure, A549 cell viability and intracellular ATP level. The lungs of rats treated with single intratracheal instillation of nano-TiO2 were injured, which was demonstrated by changes of alveolar epithelial cell ultrastructure, lung tissue pathology and lung tissue MDA level. The results of this study indicated that nano-TiO2 should be related to the generation of intracellular reactive oxygen species (ROS), which injured mitochondria and prevented the synthesis of ATP. The cells were approaching to apoptosis eventually. In macroscopic view, the lungs inevitably suffered.
authors.affiliation: Department of Biophysics, College of Basic Medical Sciences, Second Military Medical University, Xiangyin RD, Shanghai 200433, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Tang
authors.forename: Fude
authors.initials: F
authors.lastname: Wang
authors.forename: Chan
authors.initials: C
authors.lastname: Jin
authors.forename: Hao
authors.initials: H
authors.lastname: Liang
authors.forename: Xinhua
authors.initials: X
authors.lastname: Zhong
authors.forename: Yongji
authors.initials: Y
authors.lastname: Yang
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D045744:Cell Line, Tumor; D004847:Epithelial Cells / Q000187:drug effects / Q000648:ultrastructure; D006801:Humans; D008168:Lung / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D053758:Nanoparticles / Q000633:toxicity*; D011650:Pulmonary Alveoli / Q000648:ultrastructure; D051381:Rats; D014025:Titanium / Q000633:toxicity*
chemical_list: C009495:titanium dioxide; D008315:Malondialdehyde; D000255:Adenosine Triphosphate; D014025:Titanium