title: Her2neu amplification associates with Co-deletion 1p/14q in recurrent meningiomas.
abstract: BACKGROUND
  The current methods to predict recurrence and aggressive behaviour of meningiomas rely mainly on histological grading, histological subtype, proliferative index, as well as brain invasion. In many instances, histological grade alone fails to predict recurrence in the grade I and grade II meningiomas. Deletions of 1p and 14q have previously been reported to correlate with poor prognosis in terms of either recurrence or higher histological grades. The Her2neu (ErbB2) amplification has been shown to be a useful predictor of aggressive behaviour in breast and ovarian tumours, but its significance in meningioma is so far uncertain.
  
  
  METHODS
  In order to determine the cytogenetic differences between 22 recurrent and 25 non-recurrent meningiomas of all grades, we used fluorescent in situ hybridization (FISH) DNA probes for 1p36, 14q11.2 and 17q11.2-12 (Her2neu) on formalin fixed paraffin embedded (FFPE) tissue from the Brain Tumour Tissue Bank (BTTB), London Health Science Center (LHSC).
  
  
  RESULTS
  We showed a positive association for meningioma recurrence correlated with 1p36 deletion plus or minus 14q 11.2 deletions in all grades of meningiomas. The Her2neu amplification was strongly associated with 1p/14q co-deletion in cases of recurrent meningiomas, especially the higher grade tumours.
  
  
  CONCLUSIONS
  These cytogenetic markers can be applied in addition to histological grading for predicting the risk of recurrence and biological behaviour.
authors.affiliation: Department of Molecular Pathology, London Health Science Center, London, Ontario, Canada.
authors.forename: Brenda O
authors.initials: BO
authors.lastname: Hamilton
authors.forename: Joanne S
authors.initials: JS
authors.lastname: Sy
authors.forename: Joseph F
authors.initials: JF
authors.lastname: Megyesi
authors.forename: Lee Cyn
authors.initials: LC
authors.lastname: Ang
mesh_terms: D002872:Chromosome Deletion; D002878:Chromosomes, Human, Pair 1 / Q000235:genetics; D002883:Chromosomes, Human, Pair 14 / Q000235:genetics*; D003582:Cytogenetics; D005260:Female; D056726:Genetic Association Studies; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008137:Longitudinal Studies; D008297:Male; D008577:Meningeal Neoplasms / Q000235:genetics*; D008579:Meningioma / Q000235:genetics*; D009364:Neoplasm Recurrence, Local / Q000235:genetics*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics*
chemical_list: D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human