title: Identification and characterization of pheromone receptors and interplay between receptors and pheromone binding proteins in the diamondback moth, Plutella xyllostella.
abstract: Moths depend on olfactory cues such as sex pheromones to find and recognize mating partners. Pheromone receptors (PRs) and Pheromone binding proteins (PBPs) are thought to be associated with olfactory signal transduction of pheromonal compounds in peripheral olfactory reception. Here six candidate pheromone receptor genes in the diamondback moth, Plutella xyllostella were identified and cloned. All of the six candidate PR genes display male-biased expression, which is a typical characteristic of pheromone receptors. In the Xenopus-based functional study and in situ hybridization, PxylOR4 is defined as another pheromone receptor in addition to the previously characterized PxylOR1. In the study of interaction between PRs and PBPs, PxylPBPs could increase the sensitivity of the complex expressing oocyte cells to the ligand pheromone component while decreasing the sensitivity to pheromone analogs. We deduce that activating pheromone receptors in olfactory receptor neurons requires some role of PBPs to pheromone/PBP complex. If the chemical signal is not the pheromone component, but instead, a pheromone analog with a similar structure, the complex would have a decreased ability to activate downstream pheromone receptors.
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Mengjing
authors.initials: M
authors.lastname: Sun
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: William B
authors.initials: WB
authors.lastname: Walker
authors.forename: Chengcheng
authors.initials: C
authors.lastname: Liu
authors.forename: Kejian
authors.initials: K
authors.lastname: Lin
authors.forename: Shaohua
authors.initials: S
authors.lastname: Gu
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jingjiang
authors.initials: J
authors.lastname: Zhou
authors.forename: Guirong
authors.initials: G
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D057506:Arthropod Antennae / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics* / Q000378:metabolism; D055696:Olfactory Perception / Q000502:physiology; D009865:Oocytes / Q000378:metabolism; D010802:Phylogeny; D011485:Protein Binding; D043563:Receptors, Pheromone / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D015398:Signal Transduction; D014982:Xenopus laevis
chemical_list: D002352:Carrier Proteins; D019476:Insect Proteins; D043563:Receptors, Pheromone; C403713:pheromone binding protein, insect