title: Role of promoter element in c-mpl gene expression induced by TPO.
abstract: Thrombopoietin (TPO) and its receptor, c-Mpl, play the crucial role for the development of megakaryocyte and considered to regulate megakaryocytopoiesis. Previously we reported that TPO increased the c-mpl promoter activity determined by a transient expression system using a vector containing the luciferase gene as a reporter and the expression of the c-mpl gene is modulated by transcription through a protein kinase C (PKC)-dependent pathway in the megakaryoblastic cells. In this research, to elucidate the required elements in c-mpl promoter, the promoter activity of the deletion constructs and site-directed mutagenesis were measured by a transient transfection assay system. Destruction of -77GATA in c-mpl promoter decreased the activity by 22.8%. Our study elucidated that -77GATA involved in TPO-induced c-mpl gene expression in a human megakaryoblastic cell line, CMK.
authors.affiliation: Department of Anatomy, School of Life Dentistry at Tokyo, The Nippon Dental University, 1-9-20 Fujimi, Chiyoda-ku, Tokyo 102-8159, Japan. ma-suno@tky.ndu.ac.jp
authors.forename: Masataka
authors.initials: M
authors.lastname: Sunohara
authors.forename: Shigeru
authors.initials: S
authors.lastname: Morikawa
authors.forename: Akira
authors.initials: A
authors.lastname: Fuse
authors.forename: Iwao
authors.initials: I
authors.lastname: Sato
mesh_terms: D002454:Cell Differentiation / Q000502:physiology; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D007947:Leukemia, Megakaryoblastic, Acute / Q000235:genetics / Q000473:pathology / Q000503:physiopathology; D008533:Megakaryocytes / Q000473:pathology; D011401:Promoter Regions, Genetic / Q000502:physiology*; D011493:Protein Kinase C / Q000502:physiology; D053628:Receptors, Thrombopoietin / Q000235:genetics*; D011994:Recombinant Proteins / Q000494:pharmacology; D015398:Signal Transduction / Q000502:physiology; D034061:Thrombopoiesis / Q000235:genetics / Q000502:physiology*; D013926:Thrombopoietin / Q000494:pharmacology*
chemical_list: D053628:Receptors, Thrombopoietin; D011994:Recombinant Proteins; C082218:MPL protein, human; D013926:Thrombopoietin; D011493:Protein Kinase C