title: Evaluation of preincubation time interval in testicular biopsy to obtain optimum sperm parameters.
abstract: OBJECTIVE
  In sever oligospermia; one of the paths used for surgical sperm retrieval (SSR) is to extract sperm via a testicular biopsy. The aim of our study is to determine the reliable time interval between testicular biopsy and intracytoplasmic sperm injection (ICSI) procedure in order to obtain optimumsperm parameters (count, motility and normal morphology).
  
  
  METHODS
  This cohort study was carried out on 30 patients which were candidates for ICSI. After collection and keeping the samples obtained from the testicular biopsy in Ham's F10 environment, the concentration, motility and morphology of the sperm in each sample was evaluated immediately as well as 2 and 4 hours after processing. The Data were then compared with each other. For the statistical analysis, Friedman, Willcoxon and Cochran tests were used.
  
  
  RESULTS
  The mean of sperm concentration was 5.69 ± 6.14 million and the motility was10.83 ± 12.63% at 2 hours following biopsy which was significantly higher than those obtained after 0 and 4 hours of the biopsy (p <0.05).
  
  
  CONCLUSIONS
  The reliable preincubation time which resulted in the highest rate of spermatozoa parameters after testicular biopsy and before incubation was 2 hours.
authors.affiliation: 1. Fatemeh-Zahra Infertility and Reproductive Health Research Center, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Yousef-Reza
authors.initials: YR
authors.lastname: Yousefnia Pasha
authors.forename: Seyed Gholam-Ali
authors.initials: SG
authors.lastname: Jorsaraei
authors.forename: Mahtab
authors.initials: M
authors.lastname: Zeinalzadeh
authors.forename: Masoumeh
authors.initials: M
authors.lastname: Golsorkhtabar Amiri
authors.forename: Arsalan-Ali
authors.initials: AA
authors.lastname: Ramaji
keywords: Preincubation Time; Sperm Parameters; Testicular Biopsy