title: Sulforaphane controls TPA-induced MMP-9 expression through the NF-κB signaling pathway, but not AP-1, in MCF-7 breast cancer cells.
abstract: Sulforaphane [1-isothiocyanato-4-(methylsulfinyl)-butane] is an isothiocyanate found in some cruciferous vegetables, especially broccoli. Sulforaphane has been shown to display anti-cancer properties against various cancer cell lines. Matrix metalloproteinase-9 (MMP-9), which degrades the extracellular matrix (ECM), plays an important role in cancer cell invasion. In this study, we investigated the effect of sulforaphane on 12-O-tetradecanoyl phorbol-13-acetate (TPA)-induced MMP-9 expression and cell invasion in MCF-7 cells. TPA-induced MMP-9 expression and cell invasion were decreased by sulforaphane treatment. TPA substantially increased NF-κB and AP-1 DNA binding activity. Pre-treatment with sulforaphane inhibited TPA-stimulated NF-κB binding activity, but not AP-1 binding activity. In addition, we found that sulforaphane suppressed NF-κB activation, by inhibiting phosphorylation of IκB in TPA-treated MCF-7 cells. In this study, we demonstrated that the inhibition of TPA-induced MMP-9 expression and cell invasion by sulforaphane was mediated by the suppression of the NF-κB pathway in MCF-7 cells.
authors.affiliation: Department of Anesthesiology and Pain Medicine, Bucheon St. Mary's Hospital, The Catholic University of Korea, Bucheon 420-717, Korea. anes36@catholic.ac.kr
authors.forename: Young-Rae
authors.initials: YR
authors.lastname: Lee
authors.forename: Eun-Mi
authors.initials: EM
authors.lastname: Noh
authors.forename: Ji-Hey
authors.initials: JH
authors.lastname: Han
authors.forename: Jeong-Mi
authors.initials: JM
authors.lastname: Kim
authors.forename: Bo-Mi
authors.initials: BM
authors.lastname: Hwang
authors.forename: Byeong-Soo
authors.initials: BS
authors.lastname: Kim
authors.forename: Sung-Ho
authors.initials: SH
authors.lastname: Lee
authors.forename: Sung Hoo
authors.initials: SH
authors.lastname: Jung
authors.forename: Hyun Jo
authors.initials: HJ
authors.lastname: Youn
authors.forename: Eun Yong
authors.initials: EY
authors.lastname: Chung
authors.forename: Jong-Suk
authors.initials: JS
authors.lastname: Kim
mesh_terms: D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D002273:Carcinogens / Q000633:toxicity; D002465:Cell Movement / Q000187:drug effects; D006801:Humans; D051550:I-kappa B Kinase / Q000378:metabolism; D017879:Isothiocyanates; D061986:MCF-7 Cells; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D010766:Phosphorylation; D015398:Signal Transduction / Q000187:drug effects*; D013454:Sulfoxides; D013755:Tetradecanoylphorbol Acetate / Q000633:toxicity; D013861:Thiocyanates / Q000494:pharmacology*; D018808:Transcription Factor AP-1 / Q000378:metabolism
chemical_list: D016588:Anticarcinogenic Agents; D002273:Carcinogens; D017879:Isothiocyanates; D016328:NF-kappa B; D013454:Sulfoxides; D013861:Thiocyanates; D018808:Transcription Factor AP-1; D051550:I-kappa B Kinase; D020780:Matrix Metalloproteinase 9; C016766:sulforaphane; D013755:Tetradecanoylphorbol Acetate