title: Induction of broadly neutralising HCV antibodies in mice by integration-deficient lentiviral vector-based pseudotyped particles.
abstract: BACKGROUND
  Integration-deficient lentiviral vectors (IDLVs) are a promising platform for immunisation to elicit both humoral immunity and cellular mediated immunity (CMI). Here, we compared the specific immunity in mice immunised via different regimens (homologous and cocktail) with IDLV-based HCV pseudoparticles (HCVpps) carrying pseudotyped glycoproteins E1E2 and bearing the HCV NS3 gene. Humoral and cell-mediated immune responses were also evaluated after IDLV-HCVpp immunisation combined with heterologous rAd5-CE1E2 priming protocols. Sera from the mice effectively elicited anti-E1, -E2, and -NS3 antibody responses, and neutralised various HCVpp subtypes (1a, 1b, 2a, 3a and 5a). No significant CMI was detected in the groups immunised with IDLV-based HCVpps. In contrast, the combination of rAd5-CE1E2 priming and IDLV-based HCVpp boosting induced significant CMI against multiple antigens (E1, E2, and NS3).
  
  
  CONCLUSIONS
  IDLV-based HCVpps are a promising vaccination platform and the combination of rAd5-CE1E2 and IDLV-based HCVpp prime-boost strategy should be further explored for the development of a cross-protective HCV vaccine.
authors.affiliation: Key Laboratory of Medical Virology and Viral Diseases, Ministry of Health, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping District, Beijing, People's Republic of China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Deng
authors.forename: Jie
authors.initials: J
authors.lastname: Guan
authors.forename: Bo
authors.initials: B
authors.lastname: Wen
authors.forename: Na
authors.initials: N
authors.lastname: Zhu
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.forename: Jindong
authors.initials: J
authors.lastname: Song
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.forename: Wenjie
authors.initials: W
authors.lastname: Tan
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000276:immunology; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood / Q000276:immunology; D002460:Cell Line; D056738:Cross Protection / Q000276:immunology; D005260:Female; D023061:Gene Order; D005822:Genetic Vectors / Q000235:genetics*; D016174:Hepacivirus / Q000235:genetics / Q000276:immunology*; D018937:Hepatitis C Antibodies / Q000097:blood / Q000276:immunology*; D006801:Humans; D056724:Immunity, Humoral; D007114:Immunization; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D016086:Lentivirus / Q000235:genetics*; D051379:Mice; D014771:Virion / Q000276:immunology* / Q000648:ultrastructure; D016662:Virus Integration
chemical_list: D057134:Antibodies, Neutralizing; D018937:Hepatitis C Antibodies; D007074:Immunoglobulin G