title: Sugar-attached upconversion lanthanide nanoparticles: a novel tool for high-throughput lectin assay.
abstract: To create a novel high-throughput lectin assay (HTPLA) method based on the emission of a luminophore by highly penetrable near-infrared excitation, sugar-attached upconversion lanthanide nanoparticles (LNPs) were synthesized as a tool to highlight the aggregates caused by the sugar-mediated specific bridging between LNP and lectin. The emissions from a mannose-coated LNP in the aggregates with a mannose-binding lectin were much stronger than those from the non-aggregated samples, being sensitive enough for HTPLA. A galactose-coated LNP was also applicable to a macrophage aggregation assay for the sugar specificity of its surface lectin.
authors.affiliation: Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, J2-10, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.forename: Takuya
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Masayuki
authors.initials: M
authors.lastname: Iizuka
authors.forename: Tatsumi
authors.initials: T
authors.lastname: Tanaka
authors.forename: Izumi
authors.initials: I
authors.lastname: Sotokawa
authors.forename: Atsushi
authors.initials: A
authors.lastname: Shimoyama
authors.forename: Yasutoshi
authors.initials: Y
authors.lastname: Murayama
authors.forename: Eigo
authors.initials: E
authors.lastname: Otsuji
authors.forename: Shun-ichiro
authors.initials: S
authors.lastname: Ogura
authors.forename: Hideya
authors.initials: H
authors.lastname: Yuasa
mesh_terms: D000818:Animals; D002449:Cell Aggregation; D005690:Galactose / Q000737:chemistry; D057166:High-Throughput Screening Assays; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D037102:Lectins / Q000032:analysis*; D008163:Luminescent Measurements; D008264:Macrophages / Q000737:chemistry / Q000166:cytology; D008358:Mannose / Q000737:chemistry; D051379:Mice; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D019265:Spectroscopy, Near-Infrared
chemical_list: D028581:Lanthanoid Series Elements; D037102:Lectins; D008358:Mannose; D005690:Galactose