title: The triterpenoids of Ganoderma tsugae prevent stress-induced myocardial injury in mice.
abstract: Ganoderma mushrooms (Lingzhi in Chinese) have well-documented health benefits. Ganoderma tsugae (G. tsugae), one of the ganoderma species, has been commercially cultivated as a dietary supplement. Because G. tsugae has high antioxidant activity and because oxidative stress is often associated with cardiac injury, we hypothesized that G. tsugae protects against cardiac injury by alleviating oxidative stress. We tested the hypothesis using a work-overload-induced myocardial injury model created by challenging mice with isoproterenol (ISO). Remarkably, oral G. tsugae protected the mice from ISO-induced myocardial injury. Moreover, the triterpenoid fraction of G. tsugae, composed of a mixture of nine structurally related ganoderic acids (GAs), provided cardioprotection by inhibiting the ISO-induced expression of Fas/Fas ligand, oxidative stress, and apoptosis. The antioxidant activity of GAs was tested in cultured cardio-myoblast H9c2 cells against the insult of H₂O₂. GAs dissipated the cellular reactive oxygen species imposed by H₂O₂ and prevented cell death. Our findings uncovered the cardioprotective activity of G. tsugae and identified GAs as the bioactive components against cardiac insults.
authors.affiliation: Department of Cell Biology and Anatomy, College of Medicine, National Cheng Kung University, Tainan, Taiwan.
authors.forename: Qian-Yu
authors.initials: QY
authors.lastname: Kuok
authors.forename: Chen-Yu
authors.initials: CY
authors.lastname: Yeh
authors.forename: Bor-Chyuan
authors.initials: BC
authors.lastname: Su
authors.forename: Pei-Ling
authors.initials: PL
authors.lastname: Hsu
authors.forename: Hao
authors.initials: H
authors.lastname: Ni
authors.forename: Ming-Yie
authors.initials: MY
authors.lastname: Liu
authors.forename: Fan-E
authors.initials: FE
authors.lastname: Mo
mesh_terms: D000363:Agaricales / Q000737:chemistry; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D009202:Cardiomyopathies / Q000188:drug therapy*; D002460:Cell Line; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D032902:Ganoderma / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000009:adverse effects; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D020940:Reishi; D014315:Triterpenes / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D004365:Drugs, Chinese Herbal; D017382:Reactive Oxygen Species; D014315:Triterpenes; D006861:Hydrogen Peroxide
keywords: Antioxidant; Cardioprotection; Ganoderic acids; Ganoderma tsugae; Oxidative stress