title: Isolation and in vitro partial characterization of hemolytic proteins from the nematocyst venom of the jellyfish Stomolophus meleagris.
abstract: Jellyfish venom contains various toxins and can cause itching, edema, muscle aches, shortness of breath, blood pressure depression, shock or even death after being stung. Hemolytic protein is one of the most hazardous components in the venom. The present study investigated the hemolytic activity of the nematocyst venom from jellyfish Stomolophus meleagris. Anion exchange chromatography, DEAE Sepharose Fast Flow, and gel filtration chromatography, Superdex200 had been employed to isolate hemolytic proteins from the nematocyst venom of jellyfish S. meleagris. Hemolysis of chicken red blood cells was used to quantify hemolytic potency of crude nematocyst venom and chromatography fractions during the purification process. Native-PAGE profile displayed one protein band in the purified hemolytic protein (SmTX); however, two protein bands with apparent molecular weights of ≈ 45 kDa and 52 kDa were observed in the reducing SDS-PAGE analysis. Approximately 70 μg/mL of SmTX caused 50% hemolysis (HU50) of the erythrocyte suspension. The hemolytic activity of SmTX was shown to be temperature and pH dependent, with the optimum temperature and pH being 37°C and pH 5.0. The present study is the first report of isolation and partial characterization of hemolytic proteins from the nematocyst venom of the jellyfish S. meleagris. The mechanism of the hemolytic activity of SmTX is not clear and deserves further investigation.
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China.
authors.forename: Rongfeng
authors.initials: R
authors.lastname: Li
authors.forename: Huahua
authors.initials: H
authors.lastname: Yu
authors.forename: Ronge
authors.initials: R
authors.lastname: Xing
authors.forename: Song
authors.initials: S
authors.lastname: Liu
authors.forename: Yukun
authors.initials: Y
authors.lastname: Qing
authors.forename: Kecheng
authors.initials: K
authors.lastname: Li
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.forename: Xiangtao
authors.initials: X
authors.lastname: Meng
authors.forename: Jinhui
authors.initials: J
authors.lastname: Cui
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Li
mesh_terms: D000818:Animals; D002645:Chickens; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003064:Cnidarian Venoms / Q000737:chemistry / Q000633:toxicity*; D004912:Erythrocytes / Q000187:drug effects* / Q000473:pathology; D006461:Hemolysis; D006863:Hydrogen-Ion Concentration; D058894:Nematocyst; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D037401:Scyphozoa; D013696:Temperature
chemical_list: D003064:Cnidarian Venoms; D011506:Proteins
keywords: Hemolysis; Isolation; Jellyfish Stomolophus meleagris; Nematocyst venom; SmTX