title: hE-cadherin-Fc fusion protein coated surface enhances the adhesion and proliferation of human mesenchymal stem cells.
abstract: A fusion protein consisting of human E-cadherin extracellular domain and the immunoglobulin G Fc region (hE-cadherin-Fc) was prepared and used as a cell-cell adhesion biomimicking matrix for the in vitro expansion of human mesenchymal stem cells (hMSCs) for use in regenerative medicine. The hE-cadherin-Fc was stably immobilized onto a polystyrene plate due to the hydrophobicity of the Fc domain, enhancing the surface wettability and topography of the plate. The hE-cadherin-Fc matrix markedly promoted the cell adhesion and proliferation of hMSCs compared with the tissue culture-treated plate (TC-PS) and the gelatin-coated plate. Furthermore, the expanded hMSCs on the hE-cadherin-Fc were positive for CD105, similar to those from the gelatin. Additionally, the expression of E-cadherin and β-catenin in the hMSCs was improved on the hE-cadherin-Fc matrix, suggesting that the interactions of the hE-cadherin-Fc matrix with the hMSCs were substitutes for the cell-cell adhesion junctions during the initial culture stage in the absence of intercellular interactions. The hE-cadherin-Fc was shown to be a promising artificial ECM for the in vitro expansion of hMSCs.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Nankai District, Tianjin, China.
authors.forename: Jianbin
authors.initials: J
authors.lastname: Xu
authors.forename: Chuanshun
authors.initials: C
authors.lastname: Zhu
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Nan
authors.initials: N
authors.lastname: Jiang
authors.forename: Suhua
authors.initials: S
authors.lastname: Li
authors.forename: Zhongyu
authors.initials: Z
authors.lastname: Su
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Akaike
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
mesh_terms: D015820:Cadherins / Q000737:chemistry*; D002448:Cell Adhesion; D049109:Cell Proliferation; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013499:Surface Properties
chemical_list: D015820:Cadherins; D007141:Immunoglobulin Fc Fragments; D011993:Recombinant Fusion Proteins