title: Concerted expression of the thermogenic and bioenergetic mitochondrial protein machinery in brown adipose tissue.
abstract: Brown adipose tissue (BAT) is specialized in non-shivering thermogenesis through the expression of the mitochondrial uncoupling protein-1 (UCP1). In this paper, we describe the relationship between UCP1 and proteins involved in ATP synthesis. By the use of BATIRKO mice, which have enhanced UCP1 expression in BAT, an increase in ATP synthase as well as in ubiquinol cytochrome c reductase levels was observed. Alterations in mitochondrial mass or variations in ATP levels were not observed in BAT of these mice. In addition, using a protocol of brown adipocyte differentiation, the concerted expression of UCP1 with ATP synthase was found. These two scenarios revealed that increases in the uncoupling machinery of brown adypocites must be concomitantly followed by an enhancement of proteins involved in ATP synthesis. These concerted changes reflect the need to maintain ATP production in an essentially uncoupling cell type.
authors.affiliation: Faculty of Pharmacy, Department of Biochemistry and Molecular Biology II, Complutense University of Madrid, Madrid, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Guillen
authors.forename: Alberto
authors.initials: A
authors.lastname: Bartolome
authors.forename: Rocio
authors.initials: R
authors.lastname: Vila-Bedmar
authors.forename: Ana
authors.initials: A
authors.lastname: García-Aguilar
authors.forename: Almudena
authors.initials: A
authors.lastname: Gomez-Hernandez
authors.forename: Manuel
authors.initials: M
authors.lastname: Benito
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D003576:Electron Transport Complex IV / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005951:Glucose Tolerance Test; D047468:Immunoprecipitation; D007473:Ion Channels / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D024101:Mitochondrial Proteins / Q000378:metabolism*; D011972:Receptor, Insulin / Q000378:metabolism; D000071256:Uncoupling Protein 1
chemical_list: D007473:Ion Channels; D024101:Mitochondrial Proteins; C000604372:Ucp1 protein, mouse; D000071256:Uncoupling Protein 1; D000255:Adenosine Triphosphate; D003576:Electron Transport Complex IV; D011972:Receptor, Insulin
keywords: ATP SYNTHASE; BROWN ADIPOSE TISSUE; MITOCHONDRIAL BIOENERGETICS; UCP1