title: Production of ethanol from mannitol by the yeast strain Saccharomyces paradoxus NBRC 0259.
abstract: Mannitol is a promising marine macroalgal carbon source. However, organisms that produce ethanol from mannitol are limited; to date, only the yeast Pichia angophorae and the bacterium Escherichia coli KO11 have been reported to possess this capacity. In this study, we searched a yeast strain with a high capacity to produce ethanol from mannitol and selected Saccharomyces paradoxus NBRC 0259 for its ability to produce ethanol from mannitol. This ability was enhanced after a 3-day cultivation of this strain in medium containing mannitol; the enhanced strain was renamed S. paradoxus NBRC 0259-3. We compared the ability of strain NBRC 0259-3 to produce ethanol from mannitol and glucose, under several conditions, with those of P. angophorae and E. coli KO11. As a result, we concluded that S. paradoxus NBRC 0259-3 strain is the most suitable yeast strain for the production of ethanol from mannitol.
authors.affiliation: Laboratory of Basic and Applied Molecular Biotechnology, Division of Food and Biological Science, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan.
authors.forename: Anri
authors.initials: A
authors.lastname: Ota
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Kawai
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Oda
authors.forename: Keishi
authors.initials: K
authors.lastname: Iohara
authors.forename: Kousaku
authors.initials: K
authors.lastname: Murata
mesh_terms: D004926:Escherichia coli / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism*; D005427:Flocculation; D005947:Glucose / Q000378:metabolism; D008353:Mannitol / Q000378:metabolism*; D010843:Pichia / Q000378:metabolism; D012440:Saccharomyces / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D000431:Ethanol; D008353:Mannitol; D005947:Glucose
keywords: Ethanol; Flocculation; Macroalgae; Mannitol; Saccharomyces paradoxus; Yeast