title: A graphene oxide-based FRET sensor for rapid and sensitive detection of matrix metalloproteinase 2 in human serum sample.
abstract: Graphene oxide (GO) has been widely used to develop fluorescence resonance energy transfer (FRET) biosensors for tumor markers (e.g., matrix metalloproteinases, MMPs) due to its superior fluorescence quenching capacity and unique adsorption characteristics for biomolecules. In this study, fluorescein isothiocyanate-labeled peptide (Pep-FITC) was assembled onto the GO surface through covalent binding to construct a GO-Pep-FITC FRET sensor for sensitive, rapid, and accurate detection of MMP-2 in complex serum samples. Compared to similar GO-based FRET sensors fabricated through physical adsorption, the as prepared ones via covalent binding are significantly more stable under physiological conditions, enabling their detection of MMP-2 with high sensitivity (detection limit: 2.5ng/mL). More importantly, it allows for rapid MMP-2 detection (within 3h) even in complex biological samples with satisfactory accuracy and the relative standard deviation ≤7.03%. Our studies further suggest that such a platform developed here for sensitive, rapid, and accurate detection of biomarkers holds great promise for clinical diagnosis of protease-related diseases.
authors.affiliation: Key Laboratory of Luminescence and Real-Time Analysis of the Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China. eqsong@swu.edu.cn
authors.forename: Erqun
authors.initials: E
authors.lastname: Song
authors.forename: Dan
authors.initials: D
authors.lastname: Cheng
authors.forename: Yang
authors.initials: Y
authors.lastname: Song
authors.forename: Mingdong
authors.initials: M
authors.lastname: Jiang
authors.forename: Jifei
authors.initials: J
authors.lastname: Yu
authors.forename: Yunyun
authors.initials: Y
authors.lastname: Wang
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D020778:Matrix Metalloproteinase 2 / Q000097:blood / Q000302:isolation & purification*; D053758:Nanoparticles / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D010087:Oxides; D002244:Carbon; D006108:Graphite; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2