title: A divergent clade of circular single-stranded DNA viruses from pig feces.
abstract: Using metagenomics and molecular cloning methods, we characterized five novel small, circular viral genomes from pig feces that are distantly related to chimpanzee and porcine stool-associated circular viruses, (ChiSCV and PoSCV1). Phylogenetic analysis placed these viruses into a highly divergent clade of this rapidly growing new viral family. This new clade of viruses, provisionally named porcine stool-associated circular virus 2 and 3 (PoSCV2 and PoSCV3), encodes a stem-loop structure (presumably the origin of DNA replication) in the small intergenic region and a replication initiator protein commonly found in other biological systems that replicate their genomes via the rolling-circle mechanism. Furthermore, these viruses also exhibit three additional overlapping open reading frames in the large intergenic region between the capsid and replication initiator protein genes.
authors.affiliation: Virus and Prion Diseases, Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, PO Box 70, Ames, IA, 50010, USA, Andrew.Cheung@ars.usda.gov.
authors.forename: Andrew K
authors.initials: AK
authors.lastname: Cheung
authors.forename: Terry F
authors.initials: TF
authors.lastname: Ng
authors.forename: Kelly M
authors.initials: KM
authors.lastname: Lager
authors.forename: Darrell O
authors.initials: DO
authors.lastname: Bayles
authors.forename: David P
authors.initials: DP
authors.lastname: Alt
authors.forename: Eric L
authors.initials: EL
authors.lastname: Delwart
authors.forename: Roman M
authors.initials: RM
authors.lastname: Pogranichniy
authors.forename: Marcus E
authors.initials: ME
authors.lastname: Kehrli
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004267:DNA Viruses / Q000235:genetics* / Q000302:isolation & purification*; D005243:Feces / Q000821:virology*; D014644:Genetic Variation; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D013552:Swine; D013553:Swine Diseases / Q000821:virology; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D014764:Viral Proteins