title: [Effects of long-term intervention of moxa smoke on T lymphocyte subsets and CD4+ CD25+ Treg in peripheral blood of Wistar rats].
abstract: OBJECTIVE
  To investigate the cellular immune regulation of the long-term intervention of moxa smoke.
  
  
  METHODS
  Thirty-two Wistar rats were randomly divided into a blank group, a low concentration group, a medium concentration group and a high concentration group, 8 cases in each group. In addition to the blank group, rats in the other groups were exposed to the corresponding concentration moxa smoke for 20 min every day, the T lymphocyte subsets and proportion of the CD4+ CD25+ Treg in CD4+ T cells in peripheral blood were tested by flow cytometry after 6 months.
  
  
  RESULTS
  Compared with the blank group, the proportions of CD3+ CD4+, CD3+ CD8+ T cells and CD3+ CD4/CD3+ CD8+ in the other 3 moxa smoke groups were not significantly different (P > 0.05), while the proportions of the CD4+ CD25+ Treg in CD4+ T cells were significantly lower (P < 0.05), but no statistically significant differences among those 3 moxa smoke intervention groups (P > 0.05).
  
  
  CONCLUSIONS
  Long-term moxa smoke intervention has no significant effect on the proportions of CD3+ CD4+, CD3+ CD8+ T cells and CD3+ CD4+/CD3+ CD8+, but it can decrease the proportions of the CD4+ CD25+ Treg in CD4+ T cells in peripheral blood of rats. The way produced by pretreatment with moxa smoke may play immunomodulatory effect.
authors.affiliation: Acupuncture and Massage College, Beijing University of CM, Beijing 100029, China. lp.1984@163.com
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.forename: Xiu-Jie
authors.initials: XJ
authors.lastname: Pan
authors.forename: Li
authors.initials: L
authors.lastname: Han
authors.forename: Jia
authors.initials: J
authors.lastname: Yang
authors.forename: Hai
authors.initials: H
authors.lastname: Hu
authors.forename: Hong
authors.initials: H
authors.lastname: Cai
authors.forename: Ruo-Xi
authors.initials: RX
authors.lastname: Li
authors.forename: Mao-Xiang
authors.initials: MX
authors.lastname: Zhu
authors.forename: Zhi-Hua
authors.initials: ZH
authors.lastname: Yang
authors.forename: Bai-Xiao
authors.initials: BX
authors.lastname: Zhao
mesh_terms: D000818:Animals; D018655:Lymphocyte Count; D008297:Male; D009071:Moxibustion; D051381:Rats; D017208:Rats, Wistar; D012906:Smoke / Q000032:analysis*; D016176:T-Lymphocyte Subsets / Q000187:drug effects* / Q000276:immunology; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects* / Q000276:immunology; D013997:Time Factors
chemical_list: D012906:Smoke