title: MicroRNA-32 (miR-32) regulates phosphatase and tensin homologue (PTEN) expression and promotes growth, migration, and invasion in colorectal carcinoma cells.
abstract: BACKGROUND
  Colorectal carcinoma (CRC) is one of the leading causes of cancer-related mortality worldwide. MicroRNAs (miRNAs, miRs) play important roles in carcinogenesis. MiR-32 has been shown to be upregulated in CRC. In this study, we identified the potential effects of miR-32 on some important biological properties of CRC cells, and clarified the regulation of PTEN by miR-32.
  
  
  METHODS
  The effect of miR-32 on PTEN expression was assessed in CRC cell lines with miR-32 mimics/inhibitor to increase/decrease miR-32 expression. Furthermore, the roles of miR-32 in regulating CRC cells biological properties were analyzed with miR-32 mimics/inhibitor-transfected cells. The 3'-untranslated region (3'-UTR) of PTEN combined with miR-32 was verified by dual-luciferase reporter assay.
  
  
  RESULTS
  Gain-of-function and loss-of-function studies showed that overexpression of miR-32 promoted SW480 cell proliferation, migration, and invasion, reduced apoptosis, and resulted in downregulation of PTEN at a posttranscriptional level. However, miR-32 knock-down inhibited these processes in HCT-116 cells and enhanced the expression of PTEN protein. In addition, we further identified PTEN as the functional downstream target of miR-32 by directly targeting the 3'-UTR of PTEN.
  
  
  CONCLUSIONS
  Our results demonstrated that miR-32 was involved in tumorigenesis of CRC at least in part by suppression of PTEN.
authors.affiliation: Department of Gastroenterology, The affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong, China.
authors.forename: Weiyun
authors.initials: W
authors.lastname: Wu
authors.forename: Jingfang
authors.initials: J
authors.lastname: Yang
authors.forename: Xiao
authors.initials: X
authors.lastname: Feng
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Shicai
authors.initials: S
authors.lastname: Ye
authors.forename: Pengchun
authors.initials: P
authors.lastname: Yang
authors.forename: Wenkai
authors.initials: W
authors.lastname: Tan
authors.forename: Guoli
authors.initials: G
authors.lastname: Wei
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D020413:3' Untranslated Regions; D017209:Apoptosis / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology*; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D045325:HCT116 Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D051059:PTEN Phosphohydrolase / Q000235:genetics* / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; C568529:MIRN32 microRNA, human; D035683:MicroRNAs; D051059:PTEN Phosphohydrolase