title: Identification of Mycobacterium species in FFPE granulomatous lymphadenitis tissue using REBA Myco-ID®.
abstract: METHODS
  Tuberculous lymphadenitis is the most common form of lymphadenopathy; its main histopathological finding is granulomatous inflammation.
  
  
  OBJECTIVE
  A reverse blot hybridisation assay, REBA Myco-ID®, was applied to formalin-fixed paraffin-embedded (FFPE) tissue showing granulomatous lymphadenitis to define the causative agents.
  
  
  METHODS
  A total of 119 granulomatous lymphadenitis cases observed between 2000 and 2010 were studied. All tissue samples were treated by haematoxylin and eosin and Ziehl-Neelsen stain. Mycobacterium tuberculosis and non-tuberculous mycobacteria (NTM) were identified using the REBA Myco-ID assay, and resistance to rifampicin (RMP) and isoniazid (INH) was determined using REBA MTB-MDR®.
  
  
  RESULTS
  Of the 119 cases, 113 (95%) were positive with the REBA Myco-ID assay, while 20 (16.8%) were positive on acid-fast bacilli smear. Of the 113 positive REBA Myco-ID cases, 110 (92.43%) were identified as M. tuberculosis, 2 (1.7%) as NTM, and 1 (0.8%) as co-infection with M. tuberculosis and M. chelonae. Only 1 (0.9%) of the 110 M. tuberculosis cases was identified as RMP-resistant.
  
  
  CONCLUSIONS
  REBA Myco-ID is a highly sensitive and specific assay for detecting M. tuberculosis and NTM. M. tuberculosis is the main cause of granulomatous lymphadenitis.
authors.affiliation: Department of Pathology, Wonju College of Medicine, Yonsei University, Wonju, Republic of Korea.
authors.forename: J
authors.initials: J
authors.lastname: Munkhdelger
authors.forename: H-Y
authors.initials: HY
authors.lastname: Wang
authors.forename: Y
authors.initials: Y
authors.lastname: Choi
authors.forename: P M
authors.initials: PM
authors.lastname: Wairagu
authors.forename: D
authors.initials: D
authors.lastname: Lee
authors.forename: S
authors.initials: S
authors.lastname: Park
authors.forename: S
authors.initials: S
authors.lastname: Kim
authors.forename: B-Y
authors.initials: BY
authors.lastname: Jeon
authors.forename: H
authors.initials: H
authors.lastname: Lee
authors.forename: K H
authors.initials: KH
authors.lastname: Park
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000995:Antitubercular Agents / Q000494:pharmacology*; D015373:Bacterial Typing Techniques / Q000379:methods; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D007538:Isoniazid / Q000494:pharmacology; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D009161:Mycobacterium / Q000187:drug effects / Q000302:isolation & purification*; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods; D012293:Rifampin / Q000494:pharmacology; D012680:Sensitivity and Specificity; D014388:Tuberculosis, Lymph Node / Q000382:microbiology* / Q000473:pathology; D055815:Young Adult
chemical_list: D000995:Antitubercular Agents; D007538:Isoniazid; D012293:Rifampin