title: Determination of fumonisins and hydrolyzed fumonisin B1 in microbial culture media by LC/ESI-MS.
abstract: In order to analyze liquid growth media for fumonisin B1, B2 and hydrolyzed fumonisin B1 (HFB1) after incubation with microorganisms for degradation studies, a liquid chromatography/electrospray ionisation mass spectrometry (LC/ESI-MS) method was developed. The use of phytosphingosine hydrochloride (PSH) and T-2-toxin-d3 (T2d3) as internal standard has been tested. The detection limit established with PSH as an internal standard was about 20 ng/mL for FB1 and FB2 and about 50 ng/mL for HFB1. The developed method allows the rapid, simultaneous and quantitative determination of these analytes in microbial culture media without any cleanup.
authors.affiliation: Institute for Agrobiotechnology, Center for Analytical Chemistry, Konrad Lorenz Str. 20, A-3430, Tulln.
authors.forename: E
authors.initials: E
authors.lastname: Vekiru
authors.forename: E
authors.initials: E
authors.lastname: Fuchs
authors.forename: G
authors.initials: G
authors.lastname: Schatzmayr
authors.forename: M
authors.initials: M
authors.lastname: Täubel
authors.forename: E M
authors.initials: EM
authors.lastname: Binder
authors.forename: R
authors.initials: R
authors.lastname: Krska