title: Deduced sequences of the membrane fusion and attachment proteins of canine distemper viruses isolated from dogs and wild animals in Korea.
abstract: Canine distemper virus (CDV) causes highly contagious respiratory, gastrointestinal, and neurological diseases in wild and domestic animal species. Despite a broad vaccination campaign, the disease is still a serious problem worldwide. In this study, six field CDV strains were isolated from three dogs, two raccoon dogs, and one badger in Korea. The full sequence of the genes encoding fusion (F) and hemagglutinin (H) proteins were compared with those of other CDVs including field and vaccine strains. The phylogenetic analysis for the F and H genes indicated that the two CDV strains isolated from dogs were most closely related to Chinese strains in the Asia-1 genotype. Another four strains were closely related to Japanese strains in the Asia-2 genotype. The six currently isolated strains shared 90.2-92.1% and 88.2-91.8% identities with eight commercial vaccine strains in their nucleotide and amino acid sequences of the F protein, respectively. They also showed 90.1-91.4% and 87.8-90.7% identities with the same vaccine strains in their nucleotide and deduced amino acid sequences of the H protein, respectively. Different N-linked glycosylation sites were identified in the F and H genes of the six isolates from the prototype vaccine strain Onderstepoort. Collectively, these results demonstrate that at least two different CDV genotypes currently exist in Korea. The considerable genetic differences between the vaccine strains and wild-type isolates would be a major factor of the incomplete protection of dogs from CDV infections.
authors.affiliation: Department of Infectious Diseases, College of Veterinary Medicine, Konkuk University, Seoul 143-701, South Korea.
authors.forename: Chae-Wun
authors.initials: CW
authors.lastname: Bae
authors.forename: Joong-Bok
authors.initials: JB
authors.lastname: Lee
authors.forename: Seung-Yong
authors.initials: SY
authors.lastname: Park
authors.forename: Chang-Seon
authors.initials: CS
authors.lastname: Song
authors.forename: Nak-Hyung
authors.initials: NH
authors.lastname: Lee
authors.forename: Kun-Ho
authors.initials: KH
authors.lastname: Seo
authors.forename: Young-Sun
authors.initials: YS
authors.lastname: Kang
authors.forename: Choi-Kyu
authors.initials: CK
authors.lastname: Park
authors.forename: In-Soo
authors.initials: IS
authors.lastname: Choi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000835:Animals, Wild / Q000821:virology; D004216:Distemper / Q000821:virology*; D004217:Distemper Virus, Canine / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D004285:Dogs; D006389:Hemagglutinins, Viral / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D045969:Mustelidae / Q000821:virology*; D010802:Phylogeny; D046031:Raccoon Dogs / Q000821:virology*; D056910:Republic of Korea; D017386:Sequence Homology, Amino Acid; D014760:Viral Fusion Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D006389:Hemagglutinins, Viral; D014760:Viral Fusion Proteins; C076619:F protein, Phocine distemper virus