title: Chemical composition of two different extracts of berries harvested in Serbia.
abstract: Total phenolic content (TPC), total anthocyanin content (TAC), free and total ellagic acid content, sugars, minerals, and radical-scavenging activity were determined in nine berries harvested in Serbia. More than 30 phenolic compounds were identified; among them, 11 polyphenols and cis,trans-abscisic acid were quantified using UHPLC coupled with an LTQ-Orbitrap XL mass analyzer. For the first time chrysin, naringenin, pinocembrin, and galangin were quantified in some of the investigated berry species. The extraction efficiency of the two extraction systems, methanol and acetone, was investigated. It was found that acetone is a better extracting solvent for TPC, whereas more TAC was extracted by methanol. TPC in acetone extracts ranged from 177.51 to 459.71 mg gallic acid equiv/100 g frozen weight. TAC ranged from 5.39 to 96.94 mg cyanidin-3-glucoside/100 g frozen weight in methanol extracts. The amounts of both free and total ellagic acid were found to be higher in the acetone extract in comparison to the methanol extract.
authors.affiliation: Faculty of Chemistry, University of Belgrade , P.O. Box 51, 11158 Belgrade, Serbia.
authors.forename: Aleksandra V
authors.initials: AV
authors.lastname: Pavlović
authors.forename: Dragana Č
authors.initials: DČ
authors.lastname: Dabić
authors.forename: Nebojša M
authors.initials: NM
authors.lastname: Momirović
authors.forename: Biljana P
authors.initials: BP
authors.lastname: Dojčinović
authors.forename: Dušanka M
authors.initials: DM
authors.lastname: Milojković-Opsenica
authors.forename: Zivoslav Lj
authors.initials: ZLj
authors.lastname: Tešić
authors.forename: Maja M
authors.initials: MM
authors.lastname: Natić
mesh_terms: D000872:Anthocyanins / Q000032:analysis*; D002241:Carbohydrates / Q000032:analysis; D004610:Ellagic Acid / Q000032:analysis*; D044950:Flavanones / Q000032:analysis; D005419:Flavonoids / Q000032:analysis; D016166:Free Radical Scavengers / Q000032:analysis; D005638:Fruit / Q000737:chemistry*; D005960:Glucosides / Q000032:analysis; D010636:Phenols / Q000032:analysis*; D010936:Plant Extracts / Q000032:analysis*; D059808:Polyphenols / Q000032:analysis; D027824:Rosaceae / Q000737:chemistry; D055771:Serbia; D014131:Trace Elements / Q000032:analysis
chemical_list: D000872:Anthocyanins; D002241:Carbohydrates; D044950:Flavanones; D005419:Flavonoids; D016166:Free Radical Scavengers; D005960:Glucosides; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D014131:Trace Elements; C462279:cyanidin-3-O-beta-glucopyranoside; C037032:galangin; D004610:Ellagic Acid; C043561:chrysin; C016063:pinocembrin; C005273:naringenin