title: Virus spread and immune response following anterior chamber inoculation of HSV-1 lacking the Beclin-binding domain (BBD).
abstract: The autophagy response induced by HSV-1 infection is antagonized by the Beclin-binding domain (BBD). The purpose of this study was to determine if lack of the BBD affects viral spread and immune response in the eyes and brain. Our results showed that lack of the BBD increases autophagy response and activation of NLRP3 inflammasome, which in turn induces a more rapid innate immune response mediated by macrophage/microglia and NK cells in the injected eye, limiting virus replication and retinal damage. We conclude that autophagy plays a role in controlling HSV-1 infection by more rapid induction of the innate immune response.
authors.affiliation: Department of Cellular Biology and Anatomy, Medical College of Georgia, Georgia Regents University, Augusta, GA, USA. mzhang@gru.edu
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.forename: Jason
authors.initials: J
authors.lastname: Covar
authors.forename: Nancy Y
authors.initials: NY
authors.lastname: Zhang
authors.forename: Wen
authors.initials: W
authors.lastname: Chen
authors.forename: Brendan
authors.initials: B
authors.lastname: Marshall
authors.forename: Juan
authors.initials: J
authors.lastname: Mo
authors.forename: Sally S
authors.initials: SS
authors.lastname: Atherton
mesh_terms: D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000737:chemistry / Q000276:immunology*; D001343:Autophagy / Q000276:immunology; D000071186:Beclin-1; D018792:Encephalitis, Viral / Q000276:immunology / Q000821:virology; D005260:Female; D006561:Herpes Simplex / Q000276:immunology*; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000254:growth & development* / Q000276:immunology*; D007113:Immunity, Innate / Q000276:immunology; D058847:Inflammasomes / Q000276:immunology; D008565:Membrane Proteins / Q000737:chemistry / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D017434:Protein Structure, Tertiary; D012173:Retinitis / Q000276:immunology / Q000821:virology*; D014779:Virus Replication / Q000276:immunology
chemical_list: D051017:Apoptosis Regulatory Proteins; C491997:BECN1 protein, human; D000071186:Beclin-1; D058847:Inflammasomes; D008565:Membrane Proteins