title: The clinical isolate Pseudomonas aeruginosa MMA83 carries two copies of the blaNDM-1 gene in a novel genetic context.
abstract: The genetic context of the blaNDM-1 gene in the genome of Pseudomonas aeruginosa MMA83 was investigated. Sequencing of the cosmid selected for the blaNDM-1 gene revealed the presence of two blaNDM-1 copies in the genome of P. aeruginosa MMA83 in a unique genetic environment. Additionally, mating assays, DNA-DNA hybridization, and an S1 nuclease assay strongly suggest that the blaNDM-1 gene in P. aeruginosa MMA83 is chromosome borne.
authors.affiliation: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia.
authors.forename: Branko
authors.initials: B
authors.lastname: Jovcić
authors.forename: Zorica
authors.initials: Z
authors.lastname: Lepsanović
authors.forename: Jelena
authors.initials: J
authors.lastname: Begović
authors.forename: Bojan
authors.initials: B
authors.lastname: Rakonjac
authors.forename: Jelena
authors.initials: J
authors.lastname: Perovanović
authors.forename: Ljubisa
authors.initials: L
authors.lastname: Topisirović
authors.forename: Milan
authors.initials: M
authors.lastname: Kojić
mesh_terms: D001483:Base Sequence; D002874:Chromosome Mapping; D002876:Chromosomes, Bacterial / Q000235:genetics*; D003360:Cosmids; D004269:DNA, Bacterial / Q000235:genetics; D018628:Gene Dosage; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D011552:Pseudomonas Infections / Q000188:drug therapy* / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D001618:beta-Lactamases; C552864:beta-lactamase NDM-1