title: Autologous muscle-derived cells for the treatment of female stress urinary incontinence: a 2-year follow-up of a Polish investigation.
abstract: OBJECTIVE
  We evaluated the safety, feasibility and initial effects of therapy with muscle-derived cells (MDCs) for women with stress urinary incontinence (SUI).
  
  
  METHODS
  MDCs were isolated from an upper-arm muscle biopsy from 16 women with SUI. Cells were isolated by enzymatic digestion and expanded in vitro for 8-10 weeks. A quantity of 0.6-25 × 10(6) of the obtained cells were injected transurethrally into the urethral rhabdosphincter of women under local anesthesia. The cells were placed circumferentially at the 9, 12, and 3 O'clock positions with endoscopic guidance.
  
  
  RESULTS
  The initial results of the treatment of SUI with adult muscle-derived stem cells demonstrate the safety and feasibility of using these cells. The 2-year follow-up revealed a 75% success rate, with some patients achieving complete improvement (50%) and some patients achieving partial improvement (25%), suggesting that the prospects for this method are encouraging.
  
  
  CONCLUSIONS
  Stem cell therapy promises to become a minimally invasive method for the regeneration of the urethral rhabdosphincter muscle. Injecting a small number of cells does not preclude obtaining the desired therapeutic result.
authors.affiliation: Department of Gynaecology and Oncology, Jagiellonian University Collegium Medicum, Cracow, Poland.
authors.forename: Klaudia
authors.initials: K
authors.lastname: Stangel-Wojcikiewicz
authors.forename: Danuta
authors.initials: D
authors.lastname: Jarocha
authors.forename: Monika
authors.initials: M
authors.lastname: Piwowar
authors.forename: Robert
authors.initials: R
authors.lastname: Jach
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Uhl
authors.forename: Antoni
authors.initials: A
authors.lastname: Basta
authors.forename: Marcin
authors.initials: M
authors.lastname: Majka
mesh_terms: D064592:Autografts; D002478:Cells, Cultured; D004724:Endoscopy; D005240:Feasibility Studies; D005260:Female; D006801:Humans; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000166:cytology / Q000637:transplantation*; D011044:Poland; D020127:Recovery of Function; D012038:Regeneration; D033581:Stem Cell Transplantation / Q000009:adverse effects / Q000379:methods*; D013997:Time Factors; D016896:Treatment Outcome; D034941:Upper Extremity; D014521:Urethra / Q000503:physiopathology*; D001743:Urinary Bladder / Q000503:physiopathology*; D014550:Urinary Incontinence, Stress / Q000175:diagnosis / Q000503:physiopathology / Q000628:therapy*; D014563:Urodynamics
keywords: muscle-derived cells; stress urinary incontinence; therapy