title: Fourier transform infrared microspectroscopy reveals unique phenotypes for human embryonic and induced pluripotent stem cell lines and their progeny.
abstract: Fourier transform infrared (FTIR) microspectroscopy was employed to elucidate the macromolecular phenotype of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) and their differentiated progeny. Undifferentiated hESCs and hiPSC lines were found to be not clearly distinguishable from each other. However, although both hESC and hiPSC variants appeared to undergo similar changes during differentiation in terms of cell surface antigens, the derived cell types from all cell lines could be discriminated using FTIR spectroscopy. We foresee a possible future role for FTIR microspectroscopy as a powerful and objective investigative and quality control tool in regenerative medicine.
authors.affiliation: Monash Immunology and Stem Cell Laboratories, Monash University, Building 75, STRIP 1, West Ring Road, Clayton, Victoria 3800, Australia; Centre for Biospectroscopy and the School of Chemistry, Monash University, Clayton, Victoria 3800, Australia.
authors.forename: Julie
authors.initials: J
authors.lastname: Cao
authors.forename: Elizabeth S
authors.initials: ES
authors.lastname: Ng
authors.forename: Don
authors.initials: D
authors.lastname: McNaughton
authors.forename: Edouard G
authors.initials: EG
authors.lastname: Stanley
authors.forename: Andrew G
authors.initials: AG
authors.lastname: Elefanty
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Tobin
authors.forename: Philip
authors.initials: P
authors.lastname: Heraud
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D016002:Discriminant Analysis; D053595:Embryonic Stem Cells / Q000502:physiology*; D005347:Fibroblasts / Q000502:physiology; D005434:Flow Cytometry; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000502:physiology*; D016018:Least-Squares Analysis; D051379:Mice; D015999:Multivariate Analysis; D025341:Principal Component Analysis; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D000954:Antigens, Surface
keywords: Fourier transform infrared microspectroscopy; human embryonic stem cells; human induced pluripotent stem cells; regenerative medicine