title: Bone marrow processing for transplantation using Cobe Spectra cell separator.
abstract: Concentration of bone marrow aspirates is an important prerequisite prior to infusion of ABO incompatible allogeneic marrow and prior to cryopreservation and storage of autologous marrow. In this paper we present our experience in processing 15 harvested bone marrow for ABO incompatible allogeneic and autologous bone marrow (BM) transplantation using Cobe Spectra® cell separator. BM processing resulted in the median recovery of 91.5% CD34+ cells, erythrocyte depletion of 91% and volume reduction of 81%. BM processing using cell separator is safe and effective technique providing high rate of erythrocyte depletion and volume reduction, and acceptable recovery of the CD34+ cells.
authors.affiliation: Department of Transfusion Medicine, Institute for Mother and Child Health Care of Serbia, Dr. Vukan Čupić, Belgrade, Serbia. imdtransfuzija@yahoo.com
authors.forename: Dobrila
authors.initials: D
authors.lastname: Veljković
authors.forename: Olivera Šerbić
authors.initials: OŠ
authors.lastname: Nonković
authors.forename: Zorica
authors.initials: Z
authors.lastname: Radonjić
authors.forename: Miloš
authors.initials: M
authors.lastname: Kuzmanović
authors.forename: Zeljko
authors.initials: Z
authors.lastname: Zečević
mesh_terms: D000017:ABO Blood-Group System; D000293:Adolescent; D000741:Anemia, Aplastic / Q000628:therapy; D018952:Antigens, CD34 / Q000378:metabolism; D001787:Blood Group Incompatibility; D001853:Bone Marrow / Q000378:metabolism* / Q000473:pathology; D001854:Bone Marrow Cells / Q000166:cytology; D016026:Bone Marrow Transplantation / Q000379:methods*; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D004912:Erythrocytes / Q000166:cytology; D005260:Female; D006801:Humans; D007223:Infant; D015470:Leukemia, Myeloid, Acute / Q000628:therapy; D051359:Lymphohistiocytosis, Hemophagocytic / Q000628:therapy; D008228:Lymphoma, Non-Hodgkin / Q000628:therapy; D008297:Male; D009190:Myelodysplastic Syndromes / Q000628:therapy; D009447:Neuroblastoma / Q000628:therapy; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000628:therapy
chemical_list: D000017:ABO Blood-Group System; D018952:Antigens, CD34