title: In vitro cholesterol-lowering properties of Lactobacillus plantarum AN6 isolated from aji-narezushi.
abstract: Aji-narezushi is a traditional lactic acid-fermented fish. In this study, we screened for lactose-utilizing, acidophilic, bile-resistant and cholesterol-lowering lactic acid bacteria (LAB) from aji-narezushi for use as starter strains for fermented foods, as well as for use as probiotics. Of the 301 LAB isolates, 277 fermented lactose, and among these, 171 grew in de Man, Rogosa and Sharpe broth adjusted to pH 3·5. Thirty-four of the isolates were grown in a broth containing 3% (w/v) bile. All of the isolates were lactobacilli. Seven isolates that demonstrated cholesterol-lowering activity in ethanolic solution were selected. All of the isolates were identified as Lactobacillus plantarum. Lactobacillus plantarum AN6 showed the highest cholesterol-lowering activity. AN6 was more resistant to acid, salt and bile than the type strain NBRC15891(T). One-half of the cholesterol-lowering effect remained after boiling AN6 for 10 min. The Fourier transform infrared (FT-IR) analysis indicated that the content of cell wall polysaccharides in AN6 is higher than ones in the type strain. These results indicate that Lact. plantarum AN6 can be used as a profitable starter organism and probiotic.
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Tokyo, Japan. kuda@kaiyodai.ac.jp
authors.forename: T
authors.initials: T
authors.lastname: Kuda
authors.forename: T
authors.initials: T
authors.lastname: Yazaki
authors.forename: M
authors.initials: M
authors.lastname: Ono
authors.forename: H
authors.initials: H
authors.lastname: Takahashi
authors.forename: B
authors.initials: B
authors.lastname: Kimura
mesh_terms: D000818:Animals; D001646:Bile / Q000378:metabolism; D001647:Bile Acids and Salts / Q000378:metabolism; D002784:Cholesterol / Q000378:metabolism*; D005285:Fermentation; D048191:Lactiplantibacillus plantarum / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D007785:Lactose / Q000378:metabolism; D019936:Probiotics; D017747:Seafood / Q000382:microbiology*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D001647:Bile Acids and Salts; D002784:Cholesterol; D007785:Lactose
keywords: Fourier transform infrared; Lactobacillus plantarum; cholesterol lowering; narezushi