title: Anti-inflammatory and antimicrobial evaluation of neovestitol and vestitol isolated from Brazilian red propolis.
abstract: The objective of this study was to evaluate anti-inflammatory and antimicrobial activities of neovestitol and vestitol isolated from Brazilian red propolis (BRP). BRP ethanolic extract (EEP), neovestitol, and vestitol were evaluated by anti-inflammatory properties using a neutrophil migration assay. The antimicrobial activity was evaluated by minimal inhibitory and bactericidal concentrations (MIC and MBC) against Streptococcus mutans , Streptococcus sobrinus , Staphylococcus aureus , and Actinomyces naeslundii . Neovestitol, vestitol, and EEP inhibited neutrophil migration at a dose of 10 mg/kg. Regarding antimicrobial activity, neovestitol showed MICs ranging from <6.25 to 25-50 μg/mL and MBCs ranging from 25-50 to 50-100 μg/mL, while vestitol showed MICs ranging from 25-50 to 50-100 μg/mL and MBCs ranging from 25-50 to 50-100 μg/mL. Both isoflavonoids neovestitol and vestitol are consistent bioactive compounds displaying anti-inflammatory and antimicrobial activities that can strongly act in a low dose and concentration and have a promising potential to be applied in the pharmaceutical and food industries.
authors.affiliation: Department of Physiological Science, Piracicaba Dental School, University of Campinas (UNICAMP), C.P. 52, 13414-903 Piracicaba, São Paulo, Brazil.
authors.forename: Bruno
authors.initials: B
authors.lastname: Bueno-Silva
authors.forename: Severino M
authors.initials: SM
authors.lastname: Alencar
authors.forename: Hyun
authors.initials: H
authors.lastname: Koo
authors.forename: Masaharu
authors.initials: M
authors.lastname: Ikegaki
authors.forename: Gil V J
authors.initials: GV
authors.lastname: Silva
authors.forename: Marcelo H
authors.initials: MH
authors.lastname: Napimoga
authors.forename: Pedro L
authors.initials: PL
authors.lastname: Rosalen
mesh_terms: D000190:Actinomyces / Q000187:drug effects; D000818:Animals; D000890:Anti-Infective Agents / Q000302:isolation & purification* / Q000494:pharmacology; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification* / Q000494:pharmacology; D001938:Brazil; D005419:Flavonoids / Q000302:isolation & purification* / Q000494:pharmacology; D007249:Inflammation / Q000188:drug therapy; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008826:Microbial Sensitivity Tests; D011429:Propolis / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects; D013295:Streptococcus mutans / Q000187:drug effects; D016986:Streptococcus sobrinus / Q000187:drug effects; D000094162:Phytoalexins
chemical_list: D000890:Anti-Infective Agents; D000893:Anti-Inflammatory Agents; D005419:Flavonoids; D011429:Propolis; C515147:vestitol; D000094162:Phytoalexins