title: Anti-inflammatory and analgesic activities of the hydrolyzed sasanquasaponins from the defatted seeds of Camellia oleifera.
abstract: The defatted seeds of Camellia oleifera (Abel.) are used for stopping itching and pain in old days, the effective compounds need to be investigated. Sasanquasaponin as a rich fraction was extracted with 70% ethanol, purified by AB-8 macro-reticular resin, crystallized in 80% ethanol, and further hydrolyzed by 4% hydroxyl potassium or 2 M hydrochloride. Anti-inflammatory activities of the extracts were measured by carrageenan-induced paw edema in rats and croton oil induced ear inflammation in mice; the analgesic activities were analyzed by hot plate test, acetic acid induced writhing in mice; the levels of pain mediators of IL-1β, TNF-α and PGE₂ were determined; the antioxidative activities in vivo were evaluated by MDA, SOD and GSH-Px in serum of rats. The extracts showed significant (p < 0.01) anti-inflammatory and analgesic activities, remarkably (p < 0.01) inhibited production of pro-inflammatory cytokines and PGE2, decreased MDA and increased SOD and GSH-Px in serum. Inhibition of IL-1β, TNF-α and PGE2 may contribute to their anti-inflammatory and analgesic effects; elimination of free radicals is also involved. The sapogenin and acid hydrolyzed product have better anti-inflammatory, analgesic effects, and stronger antioxidative activity than sasanquasaponin and alkaline hydrolyzed product, and they are better candidate medicines for inflammation and pain.
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510640, People's Republic of China. yeyong@scut.edu.cn
authors.forename: Yong
authors.initials: Y
authors.lastname: Ye
authors.forename: Haiting
authors.initials: H
authors.lastname: Xing
authors.forename: Xuelan
authors.initials: X
authors.lastname: Chen
mesh_terms: D000700:Analgesics / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*; D028244:Camellia; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*; D004487:Edema / Q000188:drug therapy / Q000473:pathology; D005260:Female; D006868:Hydrolysis; D008297:Male; D051379:Mice; D010146:Pain / Q000188:drug therapy / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*; D012639:Seeds
chemical_list: C497504:22-O-angeloylcamelliagenin C-3-O-(glucopyranosyl-1-2)(glucopyranosyl-1-2-O-arabinopyranosyl-1-3-)glucopyranosiduronic acid; D000700:Analgesics; D000894:Anti-Inflammatory Agents, Non-Steroidal; D004365:Drugs, Chinese Herbal; D012503:Saponins