title: Proteomic changes in Bacteroides fragilis exposed to subinhibitory concentration of piperacillin/tazobactam.
abstract: Bacteroides fragilis is the anaerobe most frequently isolated from clinical specimens and piperacillin/tazobactam is among the drugs that can be used to treat polymicrobial infections in which this bacteria is often involved. During antibiotic therapy, inhibitory concentrations of antibiotics are always followed by subinhibitory concentrations which can generate phenotypic changes in bacteria. So, in this study we aimed to evaluate changes in the proteomic profile of B. fragilis grown in a sub-MIC of PTZ, using 2-D electrophoresis followed by matrix-assisted laser desorption/ionization time-of-flight/time of-flight. Analysis of the 2-DE gels showed 18 spots with significantly different volume percentages between experimental conditions and 12 were successfully identified by MS/MS. Two proteins with decreased abundance in sub-MIC condition were involved in the glycolysis (glyceraldehyde-3-phosphate dehydrogenase and triose phosphate isomerase), others two involved in amino acid metabolism (Oxoacyl-(acyl-carrier protein) synthase II and dihydrodipicolinate reductase), and finally, one protein involved in fatty acid metabolism (UDP-N-acetylglucosamine acyltransferase). Among the proteins with increased abundance, we founded three ATP synthase (alpha, beta, and alpha type V), which could be involved in antibiotic bacterial resistance by efflux pump, one protein involved in glycolysis (enolase), and one involved in protein degradation (aminoacyl-histidine dipeptidase). In conclusion, our data show overall changes in the proteome of B. fragilis conducted by sub-MIC of PTZ, whose consequences on bacterial physiology deserve further investigation.
authors.affiliation: Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, C4-204 Departamento de Microbiologia, Laboratório de Microbiologia Oral e Anaeróbios, Av. Antônio Carlos, 6627 Pampulha, CEP-31270-901 Belo Horizonte, Minas Gerais, Brazil. lucianocvbio@gmail.com
authors.forename: Luciano de Castro
authors.initials: Lde C
authors.lastname: Veloso
authors.forename: Kênia Valéria
authors.initials: KV
authors.lastname: dos Santos
authors.forename: Hélida Monteiro
authors.initials: HM
authors.lastname: de Andrade
authors.forename: Simone da Fonseca
authors.initials: Sda F
authors.lastname: Pires
authors.forename: Simone Gonçalves
authors.initials: SG
authors.lastname: dos Santos
authors.forename: Mariana Jesus
authors.initials: MJ
authors.lastname: Vaz Trindade
authors.forename: Luiz de Macêdo
authors.initials: Lde M
authors.lastname: de Farias
authors.forename: Maria Auxiliadora Roque
authors.initials: MA
authors.lastname: de Carvalho
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001441:Bacteroides fragilis / Q000737:chemistry* / Q000187:drug effects*; D024881:Drug Resistance, Bacterial / Q000187:drug effects*; D010397:Penicillanic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D010878:Piperacillin / Q000494:pharmacology; D000077725:Piperacillin, Tazobactam Drug Combination; D040901:Proteomics
chemical_list: D000900:Anti-Bacterial Agents; D000077725:Piperacillin, Tazobactam Drug Combination; D010397:Penicillanic Acid; D010878:Piperacillin
keywords: Bacteroides fragilis; Piperacillin/tazobactam; Proteome; Subinhibitory concentration; Two-dimensional electrophoresis