title: Influence of Intron II microsatellite polymorphism in human toll-like receptor 2 gene in leprosy.
abstract: Leprosy is a chronic granulomatous infection caused by the obligate intracellular organism Mycobacterium leprae. TLR2 plays a key role when activated by M. leprae lipoproteins initiating protective responses which induce bacterial killing and therefore control of disease spread. Microsatellite polymorphisms in intron2 of TLR2 gene have been reported to be associated with development of clinical features of several infectious diseases. The study aims to evaluate the influence of GT microsatellite on the expression of TLR2 which could make humans prone to M. leprae infections. A total of 279 individuals were enrolled in the study, 88 were leprosy patients, 95 were house hold contacts (HHC) and 96 were healthy controls (HC). Genotyping was done using PCR-Sequencing method. TLR2 mRNA expression was analyzed by RT-PCR. IL-10 and IFN-γ levels were measured using ELISA in MLSA stimulated cell culture supernatants. Statistical analysis was performed using Chi-Square (χ(2)) test and t-tests. Allele/genotype of TLR2 microsatellite which includes longer GT repeats was associated with low TLR2 mRNA expression and high IL-10 production while that including shorter GT repeats was associated with high TLR2 mRNA expression and low IL-10 production. High IL10 producing allele of TLR2 microsatellite might predispose house hold contacts to leprosy.
authors.affiliation: LEPRA India, Blue Peter Public Health & Research Centre, Cherlapally, Hyderabad, India. chandra.suryadevara@gmail.com
authors.forename: Naveen Chandra
authors.initials: NC
authors.lastname: Suryadevara
authors.forename: Venkata Sanjeev Kumar
authors.initials: VS
authors.lastname: Neela
authors.forename: Kamakshi Prudhula
authors.initials: KP
authors.lastname: Devalraju
authors.forename: Suman
authors.initials: S
authors.lastname: Jain
authors.forename: Krovvidi S R
authors.initials: KS
authors.lastname: SivaSai
authors.forename: Vijaya Lakshmi
authors.initials: VL
authors.lastname: Valluri
authors.forename: Subbanna
authors.initials: S
authors.lastname: Jonnalagada
authors.forename: M P J S
authors.initials: MP
authors.lastname: Anandaraj
mesh_terms: D000483:Alleles; D016022:Case-Control Studies; D018900:Dinucleotide Repeats; D015870:Gene Expression; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D016753:Interleukin-10 / Q000096:biosynthesis; D007438:Introns; D007918:Leprosy / Q000235:genetics* / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D012333:RNA, Messenger / Q000235:genetics; D051195:Toll-Like Receptor 2 / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D051195:Toll-Like Receptor 2; D016753:Interleukin-10; D007371:Interferon-gamma