title: Multi-microarray identifies lower AQP9 expression in adjuvant chemotherapy nonresponders with stage III colorectal cancer.
abstract: Approximately 25% of stage III colorectal cancer patients do not benefit from standard adjuvant chemotherapies. To identify biomarkers for nonresponders, fresh-frozen tissues of 19 nonresponders and 16 responders were analyzed with gene expression and microRNA arrays. Fifty-nine genes and 17 miRNAs were differentially expressed by at least two folds. AQP9, SATB2, and WIF1 were simultaneously lower expressed in the nonresponders and modulated by the differentially expressed miRNAs. RT-PCR validated the differential expression of AQP9 (p=0.035). In conclusion, lower AQP9 gene expression is related with non-response to adjuvant chemotherapy, showing potential as predictive marker and therapeutic target.
authors.affiliation: Department of Colorectal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
authors.forename: Ruoxu
authors.initials: R
authors.lastname: Dou
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Deng
authors.forename: Lanlan
authors.initials: L
authors.lastname: Huang
authors.forename: Shijun
authors.initials: S
authors.lastname: Fu
authors.forename: Shuyun
authors.initials: S
authors.lastname: Tan
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Lian
authors.forename: Lekun
authors.initials: L
authors.lastname: Fang
authors.forename: Xinjuan
authors.initials: X
authors.lastname: Fan
authors.forename: Gang
authors.initials: G
authors.lastname: Jin
authors.forename: Huanliang
authors.initials: H
authors.lastname: Liu
authors.forename: Jianping
authors.initials: J
authors.lastname: Wang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000970:Antineoplastic Agents / Q000494:pharmacology; D020346:Aquaporins / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism*; D017024:Chemotherapy, Adjuvant / Q000379:methods*; D015179:Colorectal Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D036961:Matrix Attachment Region Binding Proteins / Q000378:metabolism; D035683:MicroRNAs / Q000378:metabolism; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D012097:Repressor Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D016896:Treatment Outcome
chemical_list: C109669:AQP9 protein, human; D048868:Adaptor Proteins, Signal Transducing; D000970:Antineoplastic Agents; D020346:Aquaporins; D014408:Biomarkers, Tumor; D036961:Matrix Attachment Region Binding Proteins; D035683:MicroRNAs; D012097:Repressor Proteins; C521297:SATB2 protein, human; D014157:Transcription Factors; C485569:WIF1 protein, human