title: AntagomiR-27a targets FOXO3a in glioblastoma and suppresses U87 cell growth in vitro and in vivo.
abstract: OBJECTIVE
  To study the effect of the antagomiR-27a inhibitor on glioblastoma cells.
  
  
  METHODS
  The miR- 27a expression level in specimens of human glioblastoma and normal human brain tissues excised during decompression for traumatic brain injury was assessed using qRT-PCR; The predicted target gene of miR-27a was screened out through bioinformatics databases, and the predicted gene was verified using genetic report assays; the effect of antagomiR-27a on the invasion and proliferation of glioma cells was analyzed using MTT assays and 5-ethynyl-2'-deoxyuridine (EdU) labeling. A xenograft glioblastoma model in BALB-c nude mice was established to detect the effect of antagomiR-27a on tumour growth.
  
  
  RESULTS
  qRT-PCR results showed that miR-27a significantly increased in specimens from glioblastoma comparing with normal human brain tissues. Th miR-27a inhibitor significantly suppressed invasion and proliferation of glioblastoma cells. FOXO3a was verified as a new target of miR-27a by Western blotting and reporter analyzes. Tumor growth in vivo was suppressed by administration of the miR-27a inhibitor.
  
  
  CONCLUSIONS
  MiR-27a may be up-regulated in human glioblastoma, and antagomiR-27a could inhibit the proliferation and invasion ability of glioblastoma cells.
authors.affiliation: Department of Neurosurgery, Wuxi People's Hospital of Nanjing Medical University, Wuxi, China.
authors.forename: Yun-Fei
authors.initials: YF
authors.lastname: Ge
authors.forename: Jun
authors.initials: J
authors.lastname: Sun
authors.forename: Chun-Jie
authors.initials: CJ
authors.lastname: Jin
authors.forename: Bo-Qiang
authors.initials: BQ
authors.lastname: Cao
authors.forename: Zhi-Feng
authors.initials: ZF
authors.lastname: Jiang
authors.forename: Jun-Fei
authors.initials: JF
authors.lastname: Shao
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D000071316:Forkhead Box Protein O3; D051858:Forkhead Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000235:genetics; D005909:Glioblastoma / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D058990:Molecular Targeted Therapy; D009361:Neoplasm Invasiveness; D009368:Neoplasm Transplantation; D009841:Oligonucleotides / Q000235:genetics / Q000378:metabolism / Q000627:therapeutic use*; D023041:Xenograft Model Antitumor Assays
chemical_list: C499517:FOXO3 protein, human; D000071316:Forkhead Box Protein O3; D051858:Forkhead Transcription Factors; C531388:MIRN27 microRNA, human; D035683:MicroRNAs; D009841:Oligonucleotides