title: CD43 expression is associated with inferior survival in the non-germinal centre B-cell subgroup of diffuse large B-cell lymphoma.
abstract: We evaluated the prognostic significance of CD43 (SPN), a membrane glycoprotein, in 140 patients with diffuse large B-cell lymphoma (DLBCL) by tissue microarray (TMA) immunostaining, and gene expression profiling (GEP) in 43 patients. CD43 protein was expressed in 19% of the cases and was strongly related to the non-germinal centre B-cell (non-GCB) subgroup by both TMA and GEP. Patients with CD43(+) DLBCL had an inferior 3-year overall survival (OS) compared to those with CD43(-) DLBCL (50% vs. 76%, P = 0·01). Within the non-GCB subgroup, patients with CD43(+) DLBCL had a particularly poor 3-year OS (32% vs. 71%, P < 0·001). Gene set enrichment analysis within the activated B-cell subgroup revealed significant enrichment in the stromal-1 signature in CD43(-) cases. We conclude that CD43 is an adverse prognostic marker in DLBCL, and is preferentially expressed in the non-GCB subgroup. The dismal outcome of CD43(+) cases in the non-GCB subgroup may be explained, at least in part, by a less favourable microenvironment.
authors.affiliation: Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE, USA. mitrovic@mef.hr
authors.forename: Zdravko
authors.initials: Z
authors.lastname: Mitrovic
authors.forename: Javeed
authors.initials: J
authors.lastname: Iqbal
authors.forename: Kai
authors.initials: K
authors.lastname: Fu
authors.forename: Lynette M
authors.initials: LM
authors.lastname: Smith
authors.forename: Martin
authors.initials: M
authors.lastname: Bast
authors.forename: Timothy C
authors.initials: TC
authors.lastname: Greiner
authors.forename: Patricia
authors.initials: P
authors.lastname: Aoun
authors.forename: James O
authors.initials: JO
authors.lastname: Armitage
authors.forename: Julie M
authors.initials: JM
authors.lastname: Vose
authors.forename: Dennis D
authors.initials: DD
authors.lastname: Weisenburger
authors.forename: Wing C
authors.initials: WC
authors.lastname: Chan
mesh_terms: D000368:Aged; D016000:Cluster Analysis; D005260:Female; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D051917:Leukosialin / Q000235:genetics / Q000378:metabolism*; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics / Q000378:metabolism* / Q000401:mortality*; D008297:Male; D008875:Middle Aged; D011379:Prognosis
chemical_list: D051917:Leukosialin