title: Haplotypes and effects on growth traits of bovine Wnt7a gene in Chinese Qinchuan cattle.
abstract: Wnt7a is a member of the WNT gene family, which encodes secreted signaling proteins and responds to many biological processes. Specifically Wnt7a influences satellite stem cells and regulates the regenerative potential of the muscle. However, similar researches about the bovine Wnt7a gene are lacking. Therefore, in this study, polymorphisms of the bovine Wnt7a gene were detected in 488 individuals from Chinese Qinchuan cattle by DNA pooling, forced PCR-RFLP, and DNA sequencing methods. 3 novel SNPs were identified, two SNPs (g.T4926C and g.A21943G) were in the intron and the last one (g.C63777T) was in the exon. Five haplotypes involved in these three variant sites in the Wnt7a gene were identified and their effects on growth traits were analyzed. The results revealed that haplotype 1 had the highest haplotype frequencies and was highly significantly associated with body height (P<0.01), body weight (P<0.05), chest width (P<0.05) and height at hip cross (P<0.01) respectively.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xue
authors.forename: Yujia
authors.initials: Y
authors.lastname: Sun
authors.forename: Wenjiao
authors.initials: W
authors.lastname: Guo
authors.forename: Ziqi
authors.initials: Z
authors.lastname: Yang
authors.forename: Huibin
authors.initials: H
authors.lastname: Tian
authors.forename: Chunlei
authors.initials: C
authors.lastname: Zhang
authors.forename: Chuzhao
authors.initials: C
authors.lastname: Lei
authors.forename: Xianyong
authors.initials: X
authors.lastname: Lan
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
mesh_terms: D000483:Alleles; D000818:Animals; D049628:Body Size / Q000235:genetics*; D002417:Cattle; D033481:Chromosomes, Mammalian / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D056426:Genetic Loci; D056914:Genomic Structural Variation; D006239:Haplotypes; D007438:Introns; D015810:Linkage Disequilibrium; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D051153:Wnt Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D051153:Wnt Proteins