title: Development of a monoclonal antibody-based enzyme-linked immunosorbent assay for tetrabromobisphenol A.
abstract: Tetrabromobisphenol A (TBBPA) has been an important brominated flame retardant worldwide and has become a widely concerned environmental pollutant due to its persistence in the environment. In this study, a monoclonal antibody (MAb, designated 3D9G6) against TBBPA was produced, and an indirect competitive enzyme-linked immunoassay (icELISA) for detecting trace TBBPA was developed. The limit of detection and the half maximum inhibition concentration of TBBPA in phosphate-buffered saline were 0.8 and 3.87 ng·mL(-1), respectively. The assay specificity was studied with TBBPA structural analogs, such as bisphenol A, decabromodiphenyl ether, octabromobisphenol-S, and hexabromocyclododecane, and the results showed that none of these can be recognized by the MAb even at a concentration of up to 4000 ng·mL(-1). The average recoveries of TBBPA in water and soil samples were 96% and 87%, respectively. This icELISA can be applied for the detection of trace levels of TBBPA in water and soil samples.
authors.affiliation: Department of Applied Chemistry, College of Science, China Agricultural University , Beijing, People's Republic of China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Xu
authors.forename: Junjun
authors.initials: J
authors.lastname: Ou
authors.forename: Yongliang
authors.initials: Y
authors.lastname: Cui
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Cong
authors.initials: C
authors.lastname: Lv
authors.forename: Kecheng
authors.initials: K
authors.lastname: Liu
authors.forename: Baoming
authors.initials: B
authors.lastname: Wang
authors.forename: Ting
authors.initials: T
authors.lastname: Xu
authors.forename: Qing X
authors.initials: QX
authors.lastname: Li
authors.forename: Shangzhong
authors.initials: S
authors.lastname: Liu
mesh_terms: D000818:Animals; D058846:Antibodies, Monoclonal, Murine-Derived / Q000737:chemistry*; D000918:Antibody Specificity; D004797:Enzyme-Linked Immunosorbent Assay / Q000592:standards; D005260:Female; D005411:Flame Retardants / Q000032:analysis; D006241:Haptens / Q000032:analysis / Q000276:immunology*; D006825:Hybridomas; D051379:Mice; D008807:Mice, Inbred BALB C; D011075:Polybrominated Biphenyls / Q000032:analysis / Q000276:immunology*; D012015:Reference Standards; D012989:Soil Pollutants / Q000032:analysis / Q000276:immunology; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000276:immunology*
chemical_list: D058846:Antibodies, Monoclonal, Murine-Derived; D005411:Flame Retardants; D006241:Haptens; D011075:Polybrominated Biphenyls; D012989:Soil Pollutants; D014874:Water Pollutants, Chemical; C020806:tetrabromobisphenol A