title: Effect of botulinum toxin A on proliferation and apoptosis in the T47D breast cancer cell line.
abstract: The present study was performed to assess the activity of the botulinum toxin A on breast cancer cells. The T47D cell line was exposed to diverse concentrations of the botulinum toxin A and cell viability and apoptosis were estimated using MTT and propidium iodine/annexin V methods, respectively. Botulinum toxin A exerted greater cytotoxic activity in T47D cells in comparison with MCF10A normal cells; this appeared to be via apoptotic processes caspase-3 and -7. In conclusion, botulinum toxin A induces caspase-3 and -7 dependent apoptotic processes in the T47D breast cancer cell line.
authors.affiliation: Department of Research Support, Instituto Nacional de Rehabilitacion, Mexico City, Mexico.
authors.forename: Cindy
authors.initials: C
authors.lastname: Bandala
authors.forename: Jose Luis Martin
authors.initials: JL
authors.lastname: Perez-Santos
authors.forename: Eleazar
authors.initials: E
authors.lastname: Lara-Padilla
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Delgado Lopez
authors.forename: Maricruz
authors.initials: M
authors.lastname: Anaya-Ruiz
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D019274:Botulinum Toxins, Type A / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy*; D053148:Caspase 3 / Q000378:metabolism; D053179:Caspase 7 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D006801:Humans; D009465:Neuromuscular Agents / Q000494:pharmacology*
chemical_list: D009465:Neuromuscular Agents; D053148:Caspase 3; D053179:Caspase 7; D019274:Botulinum Toxins, Type A