title: In vitro and in vivo studies of a novel nanohydroxyapatite/superhydrophilic vertically aligned carbon nanotube nanocomposites.
abstract: An association between in vitro and in vivo studies has been demonstrated for the first time, using a novel nanohydroxyapatite/superhydrophilic vertically aligned multiwalled carbon nanotube (nHAp/VAMWCNT-O2) nanocomposites. Human osteoblast cell culture and bone defects were used to evaluate the in vitro extracellular matrix (ECM) calcification process and bone regeneration, respectively. The in vitro ECM calcification process of nHAp/VAMWCNT-O2 nanocomposites were investigated using alkaline phosphatase assay. The in vivo biomineralization studies were carried out on bone defects of C57BL/6/JUnib mice. Scanning electron microscopy, micro-energy dispersive spectroscopy, X-ray photoelectron spectroscopy, and X-ray difractometry analyses confirmed the presence of the nHAp crystals. nHAp/VAMWCNT-O2 nanocomposites induced in vitro calcification of the ECM of human osteoblast cells in culture after only 24 h. Bone regeneration with lamellar bone formation after 9 weeks was found in the in vivo studies. Our findings make these new nanocomposites very attractive for application in bone tissue regeneration.
authors.affiliation: Laboratory of Biomedical Nanotechnology (NANOBIO), Universidade do Vale do Paraiba (UNIVAP), Av. Shishima Hifumi 2911, Sao Jose dos Campos, SP, 12224-000, Brazil. loboao@yahoo.com
authors.forename: Anderson Oliveira
authors.initials: AO
authors.lastname: Lobo
authors.forename: Idalia A W B
authors.initials: IA
authors.lastname: Siqueira
authors.forename: Marcele F
authors.initials: MF
authors.lastname: das Neves
authors.forename: Fernanda Roberta
authors.initials: FR
authors.lastname: Marciano
authors.forename: Evaldo Jose
authors.initials: EJ
authors.lastname: Corat
authors.forename: Marcus Alexandre F
authors.initials: MA
authors.lastname: Corat
mesh_terms: D000818:Animals; D002113:Calcification, Physiologic / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D017886:Durapatite / Q000737:chemistry* / Q000494:pharmacology; D048091:Guided Tissue Regeneration / Q000295:instrumentation / Q000379:methods; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008297:Male; D008422:Materials Testing; D051379:Mice; D008810:Mice, Inbred C57BL; D053761:Nanocomposites / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods
chemical_list: D037742:Nanotubes, Carbon; D017886:Durapatite