title: Myosin-V opposes microtubule-based cargo transport and drives directional motility on cortical actin.
abstract: Intracellular transport is driven by motor proteins that either use microtubules or actin filaments as their tracks, but the interplay between these transport pathways is poorly understood. Whereas many microtubule-based motors are known to drive long-range transport, several actin-based motors have been proposed to function predominantly in cargo tethering. How these opposing activities are integrated on cargoes that contain both types of motors is unknown. Here we use inducible intracellular transport assays to show that acute recruitment of myosin-V to kinesin-propelled cargo reduces their motility near the cell periphery and enhances their localization at the actin-rich cell cortex. Myosin-V arrests rapid microtubule-based transport without the need for regulated auto- or other inhibition of kinesin motors. In addition, myosin-V, despite being an ineffective long-range transporter, can drive slow, medium-range (1-5 μm), point-to-point transport in cortical cell regions. Altogether, these data support a model in which myosin-V establishes local cortical delivery of kinesin-bound cargos through a combination of tethering and active transport.
authors.affiliation: Division of Cell Biology, Department of Biology, Faculty of Science, Utrecht University, 3584 CH Utrecht, The Netherlands. l.kapitein@uu.nl
authors.forename: Lukas C
authors.initials: LC
authors.lastname: Kapitein
authors.forename: Petra
authors.initials: P
authors.lastname: van Bergeijk
authors.forename: Joanna
authors.initials: J
authors.lastname: Lipka
authors.forename: Nanda
authors.initials: N
authors.lastname: Keijzer
authors.forename: Phebe S
authors.initials: PS
authors.lastname: Wulf
authors.forename: Eugene A
authors.initials: EA
authors.lastname: Katrukha
authors.forename: Anna
authors.initials: A
authors.lastname: Akhmanova
authors.forename: Casper C
authors.initials: CC
authors.lastname: Hoogenraad
mesh_terms: D000199:Actins / Q000378:metabolism*; D000818:Animals; D001693:Biological Transport, Active; D019556:COS Cells; D002465:Cell Movement; D002522:Chlorocebus aethiops; D016547:Kinesins / Q000378:metabolism*; D051379:Mice; D008870:Microtubules / Q000378:metabolism*; D024701:Myosin Type V / Q000378:metabolism*; D016133:Polymerase Chain Reaction
chemical_list: D000199:Actins; D024701:Myosin Type V; D016547:Kinesins