title: Ribozyme-based transfer RNA switches for post-transcriptional control of amino acid identity in protein synthesis.
abstract: Protein mutants are studied in a variety of contexts in the life sciences. However, individual mutations need to be generated in order to transcribe and translate the respective protein variants. Here, we introduce a novel strategy for controlling the incorporation of different amino acids in response to an amber stop codon by utilizing switchable designer transfer RNAs in Escherichia coli .
authors.affiliation: Department of Chemistry and Konstanz Research School Chemical Biology (KoRS-CB), University of Konstanz , Universitätsstr. 10, 78457 Konstanz, Germany.
authors.forename: Athanasios
authors.initials: A
authors.lastname: Saragliadis
authors.forename: Jörg S
authors.initials: JS
authors.lastname: Hartig
mesh_terms: D000596:Amino Acids / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D016337:RNA, Catalytic / Q000737:chemistry / Q000378:metabolism*; D012343:RNA, Transfer / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D011506:Proteins; D016337:RNA, Catalytic; D012343:RNA, Transfer