title: Time-course proteomic profile of Candida albicans during adaptation to a fetal serum.
abstract: Candida albicans is a commensal organism; however, it causes fatal diseases if the host immunity is compromised. The mortality rate is very high due to the lack of effective treatment, leading to ceaseless demand for novel pharmaceuticals. In this study, time-course proteomics of C. albicans during adaptation to fetal bovine serum (FBS) was described. Time-course proteomics is a promising way to understand the exact process of going adaptation in dynamically changing environments. Candida albicans was cultivated in yeast nitrogen base (YNB) ± FBS media, and we identified 1418 proteins in the endpoint samples incubated for 0 or 60 min by a LC-MS/MS system with a long monolithic silica capillary column. Next, we carried out time-course proteomics of the YNB + FBS samples to identify top-priority proteins for adaption to FBS. We identified 16 proteins as nascent/newly synthesized proteins, and they were recognized as candidates of important virulent factors. Gene ontology analysis revealed that transport-related proteins were enriched in the 16 proteins, indicating that C. albicans probably put priority in time on the acquisition of essential elements. Time-course proteomics of C. albicans revealed the order of priority to adapt to FBS. Depicting time-course dynamics will lead to profound understandings of virulence of C. albicans.
authors.affiliation: Japan Society for the Promotion of Science, Sakyo-ku, Kyoto, Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Aoki
authors.forename: Tomomi
authors.initials: T
authors.lastname: Ueda
authors.forename: Yohei
authors.initials: Y
authors.lastname: Tatsukami
authors.forename: Nao
authors.initials: N
authors.lastname: Kitahara
authors.forename: Hironobu
authors.initials: H
authors.lastname: Morisaka
authors.forename: Kouichi
authors.initials: K
authors.lastname: Kuroda
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Ueda
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D002176:Candida albicans / Q000737:chemistry* / Q000254:growth & development*; D002417:Cattle; D002853:Chromatography, Liquid; D003470:Culture Media / Q000737:chemistry; D005656:Fungal Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis*; D044967:Serum / Q000382:microbiology*; D053719:Tandem Mass Spectrometry; D013997:Time Factors
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D020543:Proteome