title: Drop coating deposition Raman spectroscopy of liposomes: role of cholesterol.
abstract: Drop coating deposition Raman (DCDR) spectroscopy was used to study liposomes (DPPC and asolectin) with growing proportion of cholesterol. Deposited samples of both liposomes on special hydrophobic surface formed a dried drop with a circular shape with a ring of concentrated liposomes at the outer edge. The presence of cholesterol in liposome causes a diminishing of the drop size and an increasing in diameter of the ring, but DPPC with 20% of cholesterol forms the compact drop without the ring. Raman spectra contain characteristics of both lipids and cholesterol, liposomes do not change their initial phase state after drying. Spectral mapping shows that maximum Raman intensity originated from the inner part of the ring. Our results suggest that DCDR spectroscopy can be used for studying lipids containing cholesterol in situ.
authors.affiliation: Charles University in Prague, Faculty of Mathematics and Physics, Institute of Physics, Ke Karlovu 5, Prague 2, CZ-121 16, Czech Republic. kocisova@karlov.mff.cuni.cz
authors.forename: Eva
authors.initials: E
authors.lastname: Kočišová
authors.forename: Andrej
authors.initials: A
authors.lastname: Antalík
authors.forename: Marek
authors.initials: M
authors.lastname: Procházka
mesh_terms: D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D002784:Cholesterol / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D008081:Liposomes / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry; D013059:Spectrum Analysis, Raman
chemical_list: D008081:Liposomes; D010713:Phosphatidylcholines; D015060:1,2-Dipalmitoylphosphatidylcholine; C024990:asolectin; D002784:Cholesterol
keywords: Asolectin; Cholesterol; DPPC; Drop coating deposition Raman spectroscopy; Liposome