title: X-box binding protein 1 enhances adipogenic differentiation of 3T3-L1 cells through the downregulation of Wnt10b expression.
abstract: Differentiation of preadipocytes into adipocytes is controlled by various transcription factors. Recently, the pro-adipogenic function of XBP1, a transcription factor upregulated by endoplasmic reticulum stress, has been reported. In this study, we demonstrated that XBP1 suppresses the expression of Wnt10b, an anti-adipogenic Wnt, during the differentiation of 3T3-L1 preadipocytes. The expression pattern of XBP1 was reciprocal to that of Wnt10b during the early stage of adipogenesis. The intracellular protein levels of β-catenin were negatively regulated by XBP1. Direct binding of XBP1 to the Wnt10b promoter and the subsequent decrease of the β-catenin signalling pathway represent a novel adipogenic differentiation mechanism.
authors.affiliation: Department of Biochemistry, The Catholic University of Korea, Seoul 137-701, Republic of Korea.
authors.forename: Yoon Mi
authors.initials: YM
authors.lastname: Cho
authors.forename: Dae Hun
authors.initials: DH
authors.lastname: Kim
authors.forename: Su-Nam
authors.initials: SN
authors.lastname: Kwak
authors.forename: Seong-Whan
authors.initials: SW
authors.lastname: Jeong
authors.forename: Oh-Joo
authors.initials: OJ
authors.lastname: Kwon
mesh_terms: D041721:3T3-L1 Cells; D050156:Adipogenesis; D000818:Animals; D022762:CCAAT-Enhancer-Binding Proteins / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D020868:Gene Silencing; D051379:Mice; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D000071701:Regulatory Factor X Transcription Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D051153:Wnt Proteins / Q000235:genetics / Q000378:metabolism*; D060449:Wnt Signaling Pathway; D000071717:X-Box Binding Protein 1; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism
chemical_list: D022762:CCAAT-Enhancer-Binding Proteins; C530718:CEBPA protein, mouse; D004268:DNA-Binding Proteins; D000071701:Regulatory Factor X Transcription Factors; D014157:Transcription Factors; D051153:Wnt Proteins; C102068:Wnt10b protein, mouse; D000071717:X-Box Binding Protein 1; C000605544:Xbp1 protein, mouse; D051176:beta Catenin