title: [The clinical efficiency of calf blood extract gel on moderate to severe dry eye induced by chronic graft versus host diseases after bone marrow transplantation].
abstract: OBJECTIVE
  To investigate the clinical efficiency of calf blood extract gel on dry eye induced by chronic graft versus host diseases after bone marrow transplantation.
  
  
  METHODS
  It was a two-stage cross-over design double-blind controlled study. Twelve patients (twenty-four eyes) diagnosis dry eye induced by chronic graft versus diseases in Ocular Surface Out-patient Clinic of Xiamen University Affiliated Xiamen Eye Center 2009 from 2010 to were divide into two groups: group A accepted the treatment of autologous serum in the first stage and group B accepted the treatment of calf blood extract gel, after one month of elution, group A accepted calf blood extract gel and group B accepted autologous serum. The signs and symptoms with different therapies were recorded at the time of pre and post therapies, which were analyzed by Wilcoxon analysis and two-stage cross-over analysis.
  
  
  RESULTS
  Ocular dry eye symptoms including visual tiredness, dry and unsmooth sensation, foreign body sensation, photophobia, pain, redness and visual acuity had been improved in both autologous serum therapy (U = 22.5, 43.2, 27.0, 17.4, 21.5, 38.5, 23.0, P < 0.05) and calf blood extract gel therapy (U = 333.0, 24.5, 29.0, 40.5, 26.0, 36.0, 51.0, P < 0.05) after two-week treatment. Corneal FL had significantly been improved (2.00 ± 1.00, 3.00 ± 1.50) (Group A U = 273.0, Group B U = 135.0, P < 0.01). Ocular dry eye signs and symptoms including visual tiredness, dry and unsmooth sensation, burning sensation, photophobia, pain, tearing, redness, visual acuity, corneal FL, TFBUT and S It hadn't significantly improved between two kinds of therapies (F = 1.45, 2.43, 2.14, 1.91, 1.63, 0.51, 1.19, 0.68, 2.75, 0.77, 1.23, P > 0.05) or between two kinds of offering drug orders (F = 3.17, 2.62, 0.91, 1.42, 0.89, 2.17, 0.95, 1.54, 3.21, 6.72, 1.37, P > 0.05) in the two-stage cross-over design. Only foreign body sensation had significantly statistical difference between two kinds of drug (F = 11.38, P < 0.05), while without significant statistical difference between two kinds of offering drug orders (F = 2.62, P > 0.05).
  
  
  CONCLUSIONS
  Calf blood extract gel can be consider as a alternative for the treatment of dry eye induced by cGVHDs, because of its functions on releasing ocular dry eye symptoms and promoting corneal epithelial cells repair.
authors.affiliation: Eye Institute of Xiamen University, Xiamen Eye Center Affiliated Xiamen University, Xiamen 361005, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Zu-Guo
authors.initials: ZG
authors.lastname: Liu
authors.forename: Yi
authors.initials: Y
authors.lastname: Shao
authors.forename: Jing-Yao
authors.initials: JY
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Lin
mesh_terms: D000183:Actihaemyl / Q000627:therapeutic use*; D000328:Adult; D000818:Animals; D001781:Blood Component Removal; D016026:Bone Marrow Transplantation / Q000009:adverse effects; D002417:Cattle; D018592:Cross-Over Studies; D004311:Double-Blind Method; D015352:Dry Eye Syndromes / Q000209:etiology / Q000628:therapy*; D005260:Female; D006086:Graft vs Host Disease / Q000150:complications; D006801:Humans; D008297:Male
chemical_list: D000183:Actihaemyl