title: MiR-590-5P inhibits growth of HepG2 cells via decrease of S100A10 expression and Inhibition of the Wnt pathway.
abstract: Hepatocellular carcinoma is one of the most common and lethal cancers worldwide, especially in developing countries. In the present study, we found that the expression of a microRNA, miR-590-5P, was down-regulated and S100A10 was up-regulated in six hepatocellular carcinoma cell lines. The reporter gene assay showed that overexpression of miR-590-5P effectively reduced the activity of luciferase expressed by a vector bearing the 3' untranslated region of S100A10 mRNA. Ectopic miR-590-5P overexpression mediated by lentiviral infection decreased expression of S100A10. Infection of Lv-miR-590-5P inhibited cell growth and induced cell cycle G1 arrest in HepG2 cells. In addition, miR-590-5P expression suppressed the expression of Wnt5a, cMyc and cyclin D1, and increased the phosphorylation of β-catenin and expression of Caspase 3, which may contribute to the inhibitory effect of miR-590-5P on cell growth. Taken together, our data suggest that down-regulation of miR-590-5P is involved in hepatocellular carcinoma and the restoration of miR-590-5P can impair the growth of cancer cells, suggesting that miR-590-5P may be a potential target molecule for the therapy of hepatocellular carcinoma.
authors.affiliation: Department of Geraeology, Yancheng City No.1 People's Hospital, Yancheng 224001, China. rengenf@163.com.
authors.forename: Xiangxiang
authors.initials: X
authors.lastname: Shan
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Miao
authors.forename: Rengen
authors.initials: R
authors.lastname: Fan
authors.forename: Haixin
authors.initials: H
authors.lastname: Qian
authors.forename: Ping
authors.initials: P
authors.lastname: Chen
authors.forename: Hongqi
authors.initials: H
authors.lastname: Liu
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Yan
authors.forename: Jianping
authors.initials: J
authors.lastname: Li
authors.forename: Fen
authors.initials: F
authors.lastname: Zhou
mesh_terms: D020413:3' Untranslated Regions; D017306:Annexin A2 / Q000235:genetics*; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D059447:Cell Cycle Checkpoints; D049109:Cell Proliferation; D015536:Down-Regulation; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012334:RNA, Neoplasm / Q000235:genetics; D009418:S100 Proteins / Q000235:genetics*; D015854:Up-Regulation; D060449:Wnt Signaling Pathway; D000099248:S100 Calcium Binding Protein A10
chemical_list: D020413:3' Untranslated Regions; D017306:Annexin A2; D035683:MicroRNAs; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D009418:S100 Proteins; D000099248:S100 Calcium Binding Protein A10; C568221:MIRN590 microRNA, human