title: Synthesizing oncogenic signal-processing systems that function as both "signal counters" and "signal blockers" in cancer cells.
abstract: RNA-protein interaction plays a significant role in regulating eukaryotic translation. This phenomenon raises questions about the ability of artificial biological systems to take the advantage of protein-RNA interaction. Here, we designed an oncogenic signal-processing system expressing both a Renilla luciferase reporter gene controlled by RNA-protein interaction in its 5'-untranslated region (5'-UTR) and a Firefly luciferase normalization gene. To test the ability of the designed system, we then constructed vectors targeting the nuclear factor-κB (NF-κB) or the β-catenin signal. We found that the inhibition (%) of luciferase expression was correlated to the targeted protein content, allowing quantitative measurement of oncogenic signal intensity in cancer cells. The systems inhibited the expression of oncogenic signal downstream genes and induced bladder cancer cell proliferation inhibition and apoptosis without affecting normal urothelial cells. Compared to traditional methods (ELISA and quantitative immunoblotting), the bio-systems provided highly accurate, consistent, and reproducible quantification of protein signals and were able to discriminate between cancerous and non-cancerous cells. In conclusion, the synthetic systems function as both "signal counters" and "signal blockers" in cancer cells. This approach provides a synthetic biology platform for oncogenic signal measurement and cancer treatment.
authors.affiliation: Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Sungang Road, Shenzhen 518039, China.
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Liu
authors.forename: Weiren
authors.initials: W
authors.lastname: Huang
authors.forename: Dexi
authors.initials: D
authors.lastname: Zhou
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Han
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Duan
authors.forename: Xiaoyue
authors.initials: X
authors.lastname: Zhang
authors.forename: Hu
authors.initials: H
authors.lastname: Zhang
authors.forename: Zhimao
authors.initials: Z
authors.lastname: Jiang
authors.forename: Yaoting
authors.initials: Y
authors.lastname: Gui
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Cai
mesh_terms: D045744:Cell Line, Tumor; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D023061:Gene Order; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D009369:Neoplasms / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D001749:Urinary Bladder Neoplasms / Q000235:genetics / Q000378:metabolism; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism
chemical_list: D016328:NF-kappa B; D012333:RNA, Messenger; D051176:beta Catenin; D008156:Luciferases