title: Fabrication of glucose biosensor for whole blood based on Au/hyperbranched polyester nanoparticles multilayers by antibiofouling and self-assembly technique.
abstract: Acknowledging the benefits of hyperbranched polymers and their nanoparticles, herein we report the design and synthesis of sulfonic acid group functionalized hydroxyl-terminated hyperbranched polyester (H30-SO3H) nanoparticles and their biomedical application. The H30-SO3H nanoparticles were characterized by transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy and proton nuclear magnetic resonance spectroscopy ((1)H NMR). The good hemocompatibility of H30-SO3H nanoparticles was also investigated by coagulation tests, complement activation and platelet activation. The novel glucose biosensor was fabricated by immobilizing the positively charged Au nanoparticles, H30-SO3H nanoparticles and glucose oxidase (GOx) onto the surface of glassy carbon electrode (GCE). It can be applied in whole blood directly, which was based on the good hemocompatibility and antibiofouling property of H30-SO3H nanoparticles. The biosensor had good electrocatalytic activity toward glucose with a wide linear range (0.2-20 mM), a low detection limit 1.2×10(-5) M in whole blood and good anti-interference property. The development of materials science will offer a novel platform for application to substance detection in whole blood.
authors.affiliation: Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, China.
authors.forename: Chong
authors.initials: C
authors.lastname: Sun
authors.forename: Xiaohan
authors.initials: X
authors.lastname: Chen
authors.forename: Qiaorong
authors.initials: Q
authors.lastname: Han
authors.forename: Min
authors.initials: M
authors.lastname: Zhou
authors.forename: Chun
authors.initials: C
authors.lastname: Mao
authors.forename: Qinshu
authors.initials: Q
authors.lastname: Zhu
authors.forename: Jian
authors.initials: J
authors.lastname: Shen
mesh_terms: D057219:Biofouling; D015374:Biosensing Techniques; D001786:Blood Glucose / Q000032:analysis*; D004566:Electrodes; D004579:Electron Transport; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D055767:Immobilized Proteins / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*
chemical_list: D001786:Blood Glucose; D055767:Immobilized Proteins; D011091:Polyesters; D006046:Gold; D005949:Glucose Oxidase