title: miR-19a promotes cell growth and tumorigenesis through targeting SOCS1 in gastric cancer.
abstract: Accumulating evidence has shown that microRNAs are involved in cancer development and progression. However, it remains unknown about the potential role of miR-19a in the pathogenesis of gastric cancer. Here, we report that suppressor of cytokine signaling 1 (SOCS1) is a novel target of miR-19a in gastric cancer cells and that miR-19a expression is inversely correlated with SOCS1 expression in gastric cancer cells and a subset of gastric cancer tissues. Ectopic expression of miR-19a dramatically promoted proliferation and tumorigenicity of gastric cancer cells both in vitro and in vivo. Moreover, we showed that silencing of SOCS1 promoted cell growth and colony formation resembling that of miR-19a overexpression, whereas re-introduction of SOCS1 (without the 3'-UTR) attenuated the pro-tumorigenic functions. Taken together, our findings suggest that the SOCS1 gene is a direct target of miR-19a, which functions as an oncogenic miRNA in gastric cancer by repressing the expression of tumor suppressor SOCS1.
authors.affiliation: Department of General Surgery, First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan, China. jyinglai@yahoo.com.cn
authors.forename: Shuang
authors.initials: S
authors.lastname: Qin
authors.forename: Fang
authors.initials: F
authors.lastname: Ai
authors.forename: Wei-Fang
authors.initials: WF
authors.lastname: Ji
authors.forename: Wang
authors.initials: W
authors.lastname: Rao
authors.forename: He-Cheng
authors.initials: HC
authors.lastname: Zhang
authors.forename: Wen-Jian
authors.initials: WJ
authors.lastname: Yao
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D064593:Heterografts; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D009368:Neoplasm Transplantation; D009841:Oligonucleotides / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D000071222:Suppressor of Cytokine Signaling 1 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
chemical_list: D020413:3' Untranslated Regions; C549214:MIRN19A microRNA, human; D035683:MicroRNAs; D009841:Oligonucleotides; C108944:SOCS1 protein, human; D000071222:Suppressor of Cytokine Signaling 1 Protein; D050826:Suppressor of Cytokine Signaling Proteins