title: [Detection of weak D antigen by flow cytometry].
abstract: Flow cytometry was previously applied for analysis of Rh(D) antigen density, therefore it was suggested that the flow cytometry may be used for routine detection of weak D positive phenotypes. This study was purposed to evaluate its practicability. Six weak D positive and 7 DEL individuals were detected by using saline, IAT and absorption/elution test from 2010 to 2011 years. By RHD genotyping, zygosity analysis and sequencing, 3 cases of weak D type 15, 3 cases of partial D type DVI-III and 7 cases of DEL carrying RHD1227A alleles were identified. Taking 2 normal Rh(D)-positive and 2 D-negative samples as controls, all the samples were tested by using flow cytometry, and the median fluorescence intensities were observed as well. The results indicated that all weak D type 15 and partial D type DVI samples were detected to be positive by flow cytometry, as compared with 2 Rh(D)-negative samples (P < 0.05). Seven 7 DEL samples were tested to be negative (P > 0.05), although one of 7 DEL was tested as "±" in IAT and strong positive in absorption/elution. The RHD zygosity analysis showed this DEL individual as RHD(+)/RHD(+) homozygote. It is concluded that the sensitivity of detecting D antigen by flow cytometry is similar to that of IAT, but lower than absorption/elution test. As for detecting weak D or partial D antigens, IAT is easier than flow cytometry; as for identifying DEL, the flow cytometry is not sensitive enough.
authors.affiliation: Shenzhen Blood Center, Shenzhen, Guangdong Province, China.
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Wu
authors.forename: Hong-Xian
authors.initials: HX
authors.lastname: Xu
authors.forename: Wen
authors.initials: W
authors.lastname: Xiong
authors.forename: Chao-Peng
authors.initials: CP
authors.lastname: Shao
mesh_terms: D000328:Adult; D000483:Alleles; D001782:Blood Donors; D001788:Blood Grouping and Crossmatching; D005434:Flow Cytometry / Q000379:methods*; D005838:Genotype; D006801:Humans; D010641:Phenotype; D012204:Rh-Hr Blood-Group System / Q000097:blood* / Q000235:genetics* / Q000276:immunology
chemical_list: D012204:Rh-Hr Blood-Group System; C026410:Rho(D) antigen