title: Minimally invasive bipolar fractional radiofrequency treatment upregulates anti-senescence pathways.
abstract: BACKGROUND
  The sirtuin gene family has been implicated in various anti-senescence pathways. Its connection, if any, with the skin wound healing response has yet to be elucidated.
  
  
  OBJECTIVE
  The goal of our study was to better understand the effects of FRF treatment on the sirtuin anti-senescence pathway in skin.
  
  
  METHODS
  Human abdominal skin was treated with FRF, and then harvested at 0, 2, 14, and 28 days post-treatment to assess for temporal changes in gene expression levels.
  
  
  RESULTS
  Decreased levels of SIRT1, 3, 5, and 7 were observed immediately post-FRF treatment. By Day 2, SIRT1, 6, and 7 expressions increased 50-100%. SIRT6 and 7 expression continued to increase through Day 28. Expression levels of apoptosis genes FoxO3 and p53 decreased, while Bax levels increased by Day 28.
  
  
  CONCLUSIONS
  Our results raise the possibility that sirtuin activity may be used as an accurate corollary to clinical improvement in skin quality.
authors.affiliation: University of California Berkeley, Berkeley, California, USA.
authors.forename: Sachin
authors.initials: S
authors.lastname: Rangarajan
authors.forename: Apexa
authors.initials: A
authors.lastname: Trivedi
authors.forename: Anan Abu
authors.initials: AA
authors.lastname: Ubeid
authors.forename: Basil M
authors.initials: BM
authors.lastname: Hantash
mesh_terms: D017209:Apoptosis / Q000502:physiology; D015415:Biomarkers / Q000378:metabolism; D003357:Cosmetic Techniques / Q000295:instrumentation; D004599:Electric Stimulation Therapy / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D000071316:Forkhead Box Protein O3; D051858:Forkhead Transcription Factors / Q000378:metabolism; D006801:Humans; D020133:Reverse Transcriptase Polymerase Chain Reaction; D037761:Sirtuins / Q000378:metabolism*; D012867:Skin / Q000378:metabolism*; D015595:Skin Aging / Q000502:physiology; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D015854:Up-Regulation; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: C494826:BAX protein, human; D015415:Biomarkers; C499517:FOXO3 protein, human; D000071316:Forkhead Box Protein O3; D051858:Forkhead Transcription Factors; D016159:Tumor Suppressor Protein p53; D051028:bcl-2-Associated X Protein; D037761:Sirtuins