title: Determination of iron(III) based on the fluorescence quenching of rhodamine B derivative.
abstract: A new method for determination of iron(III) has been developed using a kind of rhodamine B derivative fluorescent probe, rhodamine amide (RHA), in acidic HAc-NaAc buffer solution. In this approach, the heavy atom effect of I₃(-) was applied to quench the fluorescence of RHA. When iron(III) and KI coexisted in HAc-NaAc buffer solution, iron(III) reacted with the excess KI to produce I₃(-) that quenched the fluorescence of RHA through the formation of a non-fluorescence compound. The results showed that the fluorescence intensity decrease of RHA presented a good linear relationship with the iron(III) concentrations in the range from 0.5 to 5.0 μmol L(-1) with the correlation coefficient of 0.9970, and the detection limit was 0.3 μmol L(-1) iron(III). The approach was applied to determination of iron(III) in water samples, and the recovery was found to be from 80.7% to 100. 8%.
authors.affiliation: Key Laboratory of Analysis and Detection Technology for Food Safety, Ministry of Education, Department of Chemistry, Fuzhou University, Fuzhou 350002, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Du
authors.forename: Min
authors.initials: M
authors.lastname: Chen
authors.forename: Yingxue
authors.initials: Y
authors.lastname: Zhang
authors.forename: Feng
authors.initials: F
authors.lastname: Luo
authors.forename: Chunyan
authors.initials: C
authors.lastname: He
authors.forename: Meijin
authors.initials: M
authors.lastname: Li
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
mesh_terms: D005290:Ferric Compounds / Q000032:analysis*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000032:analysis*; D057230:Limit of Detection; D011193:Potassium Iodide / Q000737:chemistry; D012235:Rhodamines / Q000737:chemistry*; D012996:Solutions; D013050:Spectrometry, Fluorescence / Q000379:methods; D014867:Water / Q000737:chemistry*
chemical_list: D005290:Ferric Compounds; D005456:Fluorescent Dyes; D012235:Rhodamines; D012996:Solutions; D014867:Water; D011193:Potassium Iodide; D007501:Iron