title: Globular adiponectin modulates expression of programmed cell death 4 and miR-21 in RAW 264.7 macrophages through the MAPK/NF-κB pathway.
abstract: MicroRNA-21 and programmed cell death 4 (PDCD4), a downstream target of miR-21, mediate diverse physiological responses. Here we demonstrate that globular adiponectin (gAcrp) modulates expression of miR-21 and PDCD4 in RAW 264.7 macrophages. These effects were abrogated by inhibitors of ERK1/2, JNK or NF-κB. Conditioned media collected from gAcrp-stimulated RAW 264.7 macrophages caused similar effects as direct gAcrp treatment, showing the paracrine effect of gAcrp. These data indicate that gAcrp modulates the miR-21/PDCD4 axis through the ERK and JNK/NF-κB pathways in RAW 264.7 macrophages and further suggest that the miR-21/PDCD4 axis may be a novel target mediating adiponectin-induced biological responses.
authors.affiliation: College of Pharmacy, Yeungnam University, Gyeongsanbuk-do 712-749, Republic of Korea.
authors.forename: Amit
authors.initials: A
authors.lastname: Subedi
authors.forename: Mi Jin
authors.initials: MJ
authors.lastname: Kim
authors.forename: Saroj
authors.initials: S
authors.lastname: Nepal
authors.forename: Eung-Seok
authors.initials: ES
authors.lastname: Lee
authors.forename: Jung-Ae
authors.initials: JA
authors.lastname: Kim
authors.forename: Dong-Hwan
authors.initials: DH
authors.lastname: Sohn
authors.forename: Kyung
authors.initials: K
authors.lastname: Song
authors.forename: Sung Hee
authors.initials: SH
authors.lastname: Lee
authors.forename: Won Sang
authors.initials: WS
authors.lastname: Park
authors.forename: Byeong-Seon
authors.initials: BS
authors.lastname: Jeong
authors.forename: Pil-Hoon
authors.initials: PH
authors.lastname: Park
mesh_terms: D052242:Adiponectin / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology* / Q000502:physiology; D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000235:genetics* / Q000378:metabolism; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D019899:Paracrine Communication / Q000502:physiology; D017510:Protein Folding; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D052242:Adiponectin; C499919:Adipoq protein, mouse; D051017:Apoptosis Regulatory Proteins; D017077:Culture Media, Conditioned; C531950:MIRN21 microRNA, mouse; D035683:MicroRNAs; D016328:NF-kappa B; C498443:Pdcd4 protein, mouse; D016601:RNA-Binding Proteins