title: Protective effect of ethyl acetate fraction of Acacia ferruginea DC. against ethanol-induced gastric ulcer in rats.
abstract: BACKGROUND
  In traditional systems of medicine, stem bark of Acacia ferruginea DC. is used for the treatment of itching, leucoderma, ulcers, stomatitis and diseases of the blood. In the present study, we determined antioxidant and anti-ulcerogenic activities of Acacia ferruginea stem bark.
  
  
  METHODS
  Acetone extract and its sub-fractions of Acacia ferruginea stem bark were subjected to assess their antioxidant potential using various in vitro systems such as DPPH(•), ABTS(•+) scavenging, FRAP and phosphomolybdenum reduction activities. Based on the antioxidant potential, the ethyl acetate fraction was used to evaluate the protective effect of ethanol-induced gastric damage in rat model. Enzyme activities such as superoxide dismutase, glutathione, catalase and lipid peroxidation were also determined in the stomach tissues.
  
  
  RESULTS
  Ethyl acetate fraction (AFE) of Acacia ferruginea stem bark registered higher antioxidant and free radical scavenging activities than the crude acetone extract and other fractions. In addition, AFE exhibited that the IC50 values of DPPH (2.5µg/ml) and ABTS (1.8µg/ml) were lower when compared to the standard quercetin (12.4µg/ml and 4.7µg/ml, respectively). In ethanol induced gastric ulcer, administration of AFE at doses of 10mg/kg, 50mg/kg and 100mg/kg body weight prior to ethanol ingestion significantly protected the stomach ulceration. Consequently significant changes were observed in enzyme activities such as SOD, CAT, GSH and LPO in the stomach tissues when compared with ethanol control group.
  
  
  CONCLUSIONS
  It is concluded that the ethyl acetate fraction of Acacia ferruginea stem bark possessed higher antioxidant and anti-ulcerogenic activities. Based on the results, we suggest that Acacia ferruginea stem bark has potential to provide a therapeutic approach to ethanol mediated ulcer as an effective anti-ulcer agent.
authors.affiliation: Department of Biotechnology, Daegu University, Gyeongsan, Gyeongbuk 712-714, Republic of Korea.
authors.forename: Kandhasamy
authors.initials: K
authors.lastname: Sowndhararajan
authors.forename: Sun Chul
authors.initials: SC
authors.lastname: Kang
mesh_terms: D000045:Acacia; D000085:Acetates / Q000737:chemistry; D000096:Acetone / Q000737:chemistry; D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology / Q000627:therapeutic use*; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use*; D002374:Catalase / Q000378:metabolism; D000431:Ethanol; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008517:Phytotherapy; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D012997:Solvents / Q000737:chemistry; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000085:Acetates; D000897:Anti-Ulcer Agents; D000975:Antioxidants; D010936:Plant Extracts; D012997:Solvents; D000096:Acetone; D000431:Ethanol; C007650:ethyl acetate; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione