title: The effect of low and high plasma levels of insulin-like growth factor-1 (IGF-1) on the morphology of major organs: studies of Laron dwarf and bovine growth hormone transgenic (bGHTg) mice.
abstract: It is well known that somatotrophic/insulin signaling affects lifespan in experimental animals. To study the effects of insulin-like growth factor-1 (IGF-1) plasma level on the morphology of major organs, we analyzed lung, heart, liver, kidney, bone marrow, and spleen isolated from 2-year-old growth hormone receptor knockout (GHR-KO) Laron dwarf mice (with low circulating plasma levels of IGF-1) and 6-month-old bovine growth hormone transgenic (bGHTg) mice (with high circulating plasma levels of IGF-1). The ages of the two mutant strains employed in our studies were selected based on their overall ~50% survival (Laron dwarf mice live up to ~4 years and bGHTg mice up to ~1 year). Morphological analysis of the organs of long-living 2-year-old Laron dwarf mice revealed a lower biological age for their organs compared with normal littermates, with more brown adipose tissue (BAT) surrounding the main body organs, lower levels of steatosis in liver, and a lower incidence of leukocyte infiltration in different organs. By contrast, the organs of 6-month-old, short-living bGHTg mice displayed several abnormalities in liver and kidney and a reduced content of BAT around vital organs.
authors.affiliation: Department of Physiology Pomeranian Medical University, Szczecin, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Piotrowska
authors.forename: Sylwia J
authors.initials: SJ
authors.lastname: Borkowska
authors.forename: Barbara
authors.initials: B
authors.lastname: Wiszniewska
authors.forename: Maria
authors.initials: M
authors.lastname: Laszczyńska
authors.forename: Sylwia
authors.initials: S
authors.lastname: Słuczanowska-Głabowska
authors.forename: Aaron M
authors.initials: AM
authors.lastname: Havens
authors.forename: John J
authors.initials: JJ
authors.lastname: Kopchick
authors.forename: Andrzej
authors.initials: A
authors.lastname: Bartke
authors.forename: Russel S
authors.initials: RS
authors.lastname: Taichman
authors.forename: Magda
authors.initials: M
authors.lastname: Kucia
authors.forename: Mariusz Z
authors.initials: MZ
authors.lastname: Ratajczak
mesh_terms: D002001:Adipose Tissue, Brown / Q000473:pathology; D000818:Animals; D002417:Cattle; D003720:Densitometry; D005234:Fatty Liver / Q000473:pathology; D013006:Growth Hormone / Q000235:genetics / Q000378:metabolism; D022781:Hepatocytes / Q000166:cytology; D007334:Insulin-Like Growth Factor I / Q000096:biosynthesis* / Q000502:physiology*; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics; D007668:Kidney / Q000473:pathology; D007962:Leukocytes / Q000166:cytology; D008099:Liver / Q000473:pathology; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D010729:Phosphoenolpyruvate Carboxykinase (GTP) / Q000235:genetics; D010949:Plasma / Q000378:metabolism; D051381:Rats; D013154:Spleen / Q000473:pathology; D013997:Time Factors; D014018:Tissue Distribution
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; C073294:growth hormone, bovine; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone; C582554:Pck1 protein, rat; D010729:Phosphoenolpyruvate Carboxykinase (GTP)