title: Deficiency in IL-17-committed Vγ4(+) γδ T cells in a spontaneous Sox13-mutant CD45.1(+) congenic mouse substrain provides protection from dermatitis.
abstract: Interleukin 17 (IL-17)-committed γδ T cells (γδT17 cells) participate in many immune responses, but their developmental requirements and subset specific functions remain poorly understood. Here we report that a commonly used CD45.1(+) congenic C57BL/6 mouse substrain is characterized by selective deficiency in Vγ4(+) γδT17 cells. This trait was due to a spontaneous mutation in the gene encoding the transcription factor Sox13 that caused an intrinsic defect in development of those cells in the neonatal thymus. The γδT17 cells migrated from skin to lymph nodes at low rates. In a model of psoriasis-like dermatitis, the Vγ4(+) γδT17 cell subset expanded considerably in lymph nodes and homed to inflamed skin. Sox13-mutant mice were protected from psoriasis-like skin changes, which identified a role for Sox13-dependent γδT17 cells in this inflammatory condition.
authors.affiliation: Howard Hughes Medical Institute, University of California San Francisco, San Francisco, California, USA.
authors.forename: Elizabeth E
authors.initials: EE
authors.lastname: Gray
authors.forename: Francisco
authors.initials: F
authors.lastname: Ramírez-Valle
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.forename: Shuang
authors.initials: S
authors.lastname: Wu
authors.forename: Zhihao
authors.initials: Z
authors.lastname: Wu
authors.forename: Klaus E
authors.initials: KE
authors.lastname: Karjalainen
authors.forename: Jason G
authors.initials: JG
authors.lastname: Cyster
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001324:Autoantigens / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D002478:Cells, Cultured; D003872:Dermatitis / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D005434:Flow Cytometry; D020381:Interleukin-17 / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D008198:Lymph Nodes / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D051379:Mice; D020297:Mice, Congenic; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D016688:Mice, Inbred NOD; D018345:Mice, Knockout; D009154:Mutation; D011565:Psoriasis / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000276:immunology* / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D013950:Thymus Gland / Q000276:immunology / Q000378:metabolism / Q000473:pathology
chemical_list: D001324:Autoantigens; D020381:Interleukin-17; D016692:Receptors, Antigen, T-Cell, gamma-delta; C110455:Sox13 protein, mouse; D017493:Leukocyte Common Antigens; C516770:Ptprc protein, mouse