title: Detection of foot-and-mouth disease serotype O by ELISA using a monoclonal antibody.
abstract: An ELISA assay with monoclonal antibody (MELISA) was used to type serotype O of foot-and-mouth disease virus (FMDV). All FMDV serotype O reference strains were positive by MELISA, while other viruses such as FMDV serotypes Asia 1, C, and A and classical swine fever virus, swine vesicular disease virus, and porcine reproductive and respiratory syndrome virus remained negative. Furthermore, FMDV serotype O positive samples were able to be detected by MELISA. This assay may be particularly suitable for diagnosis of FMDV serotype O infection in field stations.
authors.affiliation: College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, 730070, Gansu, PR China. haotaichen@163.com
authors.forename: Hao-Tai
authors.initials: HT
authors.lastname: Chen
authors.forename: Yun-Hua
authors.initials: YH
authors.lastname: Peng
authors.forename: Yong-Guang
authors.initials: YG
authors.lastname: Zhang
authors.forename: Xiang-Tao
authors.initials: XT
authors.lastname: Liu
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D005536:Foot-and-Mouth Disease / Q000175:diagnosis*; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics / Q000302:isolation & purification*; D013045:Species Specificity
chemical_list: D000911:Antibodies, Monoclonal