title: Fast incorporation of primary amine group into polylactide surface for improving C₂C₁₂ cell proliferation using nitrogen-based atmospheric-pressure plasma jets.
abstract: In this article, we report the development of the fast incorporation of primary amine functional groups into a polylactide (PLA) surface using the post-discharge jet region of an atmospheric-pressure nitrogen-based dielectric barrier discharge (DBD). Plasma treatments were carried out in two sequential steps: (1) nitrogen with 0.1% oxygen addition, and (2) nitrogen with 5% ammonia addition. The analyses show that the concentration of N/C ratio, surface energy, contact angle, and surface roughness of the treated PLA surface can reach 19.1%, 70.5 mJ/m(2), 38° and 73.22 nm, respectively. In addition, the proposed two-step plasma treatment procedure can produce a PLA surface exhibiting almost the same C2C12 cell attachment and proliferation performance as that of the conventional gelatin coating method. Most importantly, the processing/preparation time is reduced from 13-15 h (gelatin coating method) to 5-15 min (two-step plasma treatment), which is very useful in practical applications.
authors.affiliation: Department of Mechanical Engineering, National Chiao Tung University, Hsinchu, Taiwan.
authors.forename: Yi-Wei
authors.initials: YW
authors.lastname: Yang
authors.forename: Jane-Yii
authors.initials: JY
authors.lastname: Wu
authors.forename: Chih-Tung
authors.initials: CT
authors.lastname: Liu
authors.forename: Guo-Chun
authors.initials: GC
authors.lastname: Liao
authors.forename: Hsuan-Yu
authors.initials: HY
authors.lastname: Huang
authors.forename: Ray-Quen
authors.initials: RQ
authors.lastname: Hsu
authors.forename: Ming-Hung
authors.initials: MH
authors.lastname: Chiang
authors.forename: Jong-Shinn
authors.initials: JS
authors.lastname: Wu
mesh_terms: D000818:Animals; D001274:Atmospheric Pressure; D002448:Cell Adhesion; D002460:Cell Line; D049109:Cell Proliferation; D051379:Mice; D009584:Nitrogen / Q000737:chemistry*; D058626:Plasma Gases / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D013499:Surface Properties; D013997:Time Factors
chemical_list: D058626:Plasma Gases; D011091:Polyesters; C033616:poly(lactide); D009584:Nitrogen
keywords: DBD; PLA; atmospheric‐pressure; biomaterial; cell proliferation; plasma; tissue engineering