title: Pump-probe nonlinear phase dispersion spectroscopy.
abstract: Pump-probe microscopy is an imaging technique that delivers molecular contrast of pigmented samples. Here, we introduce pump-probe nonlinear phase dispersion spectroscopy (PP-NLDS), a method that leverages pump-probe microscopy and spectral-domain interferometry to ascertain information from dispersive and resonant nonlinear effects. PP-NLDS extends the information content to four dimensions (phase, amplitude, wavelength, and pump-probe time-delay) that yield unique insight into a wider range of nonlinear interactions compared to conventional methods. This results in the ability to provide highly specific molecular contrast of pigmented and non-pigmented samples. A theoretical framework is described, and experimental results and simulations illustrate the potential of this method. Implications for biomedical imaging are discussed.
authors.affiliation: Department of Chemistry, Duke University, Durham, North Carolina 27708, USA. francisco.robles@duke.edu
authors.forename: Francisco E
authors.initials: FE
authors.lastname: Robles
authors.forename: Prathyush
authors.initials: P
authors.lastname: Samineni
authors.forename: Jesse W
authors.initials: JW
authors.lastname: Wilson
authors.forename: Warren S
authors.initials: WS
authors.lastname: Warren
mesh_terms: D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008853:Microscopy / Q000295:instrumentation*; D057054:Molecular Imaging / Q000295:instrumentation*; D017711:Nonlinear Dynamics; D013057:Spectrum Analysis / Q000295:instrumentation*