title: NMR characterization of the interaction of GroEL with amyloid β as a model ligand.
abstract: Here we report an NMR study on the substrate interaction modes of GroEL using amyloid β (Aβ) as a model ligand. We found that GroEL could suppress Aβ(1-40) amyloid formation by interacting with its two hydrophobic segments Leu17-Ala21 and Ala30-Val36, which involve key residues in fibril formation. The binding site of Aβ(1-40) was mapped on a pair of α-helices located in the GroEL apical domain. These results provide insights into chaperonin recognition of amyloidogenic proteins of pathological interest.
authors.affiliation: Institute for Molecular Science and Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences, Myodaiji, Okazaki, Japan.
authors.forename: Maho
authors.initials: M
authors.lastname: Yagi-Utsumi
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kunihara
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakamura
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Uekusa
authors.forename: Koki
authors.initials: K
authors.lastname: Makabe
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Kuwajima
authors.forename: Koichi
authors.initials: K
authors.lastname: Kato
mesh_terms: D000595:Amino Acid Sequence; D000682:Amyloid / Q000737:chemistry; D016229:Amyloid beta-Peptides / Q000737:chemistry*; D001665:Binding Sites; D018834:Chaperonin 60 / Q000737:chemistry*; D029968:Escherichia coli Proteins / Q000737:chemistry*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry*; D010449:Peptide Mapping; D054730:Protein Interaction Domains and Motifs; D055503:Protein Multimerization; D017433:Protein Structure, Secondary
chemical_list: D000682:Amyloid; D016229:Amyloid beta-Peptides; D018834:Chaperonin 60; D029968:Escherichia coli Proteins; D010446:Peptide Fragments; C078592:amyloid beta-protein (1-40)