title: EFA6 activates Arf6 and participates in its targeting to the Flemming body during cytokinesis.
abstract: The small GTPase Arf6 is transiently associated with the ingressing cleavage furrow and subsequently targeted to the Flemming body during cytokinesis, suggesting its activation around the cleavage furrow. Here, we show that EFA6 (exchange factor for Arf6) localizes on the cleavage furrow through its PH domain. Time-lapse analysis showed that both EFA6 and Arf6 are transiently localized around the ingressing cleavage furrow, but only Arf6 is subsequently targeted to the Flemming body. Expression of an EFA6 mutant suppresses Arf6 recruitment onto the Flemming body. These results suggest that EFA6 participates in activation of Arf6 around the cleavage furrow during cytokinesis.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Ueda
authors.forename: Ayako
authors.initials: A
authors.lastname: Hanai
authors.forename: Tomomi
authors.initials: T
authors.lastname: Takei
authors.forename: Keiji
authors.initials: K
authors.lastname: Kubo
authors.forename: Minako
authors.initials: M
authors.lastname: Ohgi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Sakagami
authors.forename: Senye
authors.initials: S
authors.lastname: Takahashi
authors.forename: Hye-Won
authors.initials: HW
authors.lastname: Shin
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Nakayama
mesh_terms: D000089982:ADP-Ribosylation Factor 6; D020727:ADP-Ribosylation Factors / Q000378:metabolism*; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D017124:Conserved Sequence; D048749:Cytokinesis; D004789:Enzyme Activation; D020662:Guanine Nucleotide Exchange Factors; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D016297:Mutagenesis, Site-Directed; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D059010:Single-Cell Analysis; D059008:Time-Lapse Imaging
chemical_list: D000089982:ADP-Ribosylation Factor 6; D020662:Guanine Nucleotide Exchange Factors; D009419:Nerve Tissue Proteins; C109970:PSD protein, human; D020727:ADP-Ribosylation Factors; C000715348:ARF6 protein, human