title: Comparision of anti-HLA antibodies of kidney transplant candidates with chronic renal failure by two different methods: Flow-PRA and Luminex PRA.
abstract: OBJECTIVE
  The aim of the study was to compare anti-HLA antibodies examined as panel-reactive antibody (PRA) in kidney transplant candidates with chronic renal failure (CRF) with the use of 2 methods: Flow-PRA and Luminex-PRA.
  
  
  METHODS
  CRF patients displaying class I PRA (n = 34) and/or class II PRA (n = 41) were tested by the 2 different methods from April 2012 to September 2012, using antigen-coated beads.
  
  
  RESULTS
  Eleven (32.3%) 34 patients tested for class I PRA were female and 23 (67.7%) male; 17 (41.5%) 41 patients tested for class II PRA were female and 24 (58.5%) male. Only 2 patients were preemptive, the others had been subjected to dialysis. The concordance ratio of class I PRA test results between Flow-PRA and Luminex PRA was 67.6%. Whereas 13 samples (38.2%) were positive by Flow-PRA, 22 (64.7%) were positive by Luminex-PRA. Two of the 3 patients not previously immunized were found to be positive only by Luminex PRA; 1 was noted to be positive only by Flow-PRA. Regarding class II PRA screening, the concordance between Flow-PRA and Luminex PRA was 70.7%. Whereas 14 (34.1%) samples were positive for Flow-PRA; 24 (58.5%) were positive for Luminex-PRA. The 2 patients not previously immunized were positive only in Luminex PRA.
  
  
  CONCLUSIONS
  We speculated that the reason for the low concordance ratios was due to the use of sera that had been previously found to be indeterminate in PRA tests. We also speculated that the low concordance ratios were due to the coating procedure for the beads, which may cause changes in antigenic epitopes and decrease concordance between Flow-PRA and Luminex-PRA.
authors.affiliation: Tissue Typing Laboratory, Tepecik Training and Research Hospital, Izmir, Turkey. dryusuf@istanbul.com
authors.forename: Y
authors.initials: Y
authors.lastname: Kurtulmuş
authors.forename: T K
authors.initials: TK
authors.lastname: Ayna
authors.forename: M
authors.initials: M
authors.lastname: Soyöz
authors.forename: B
authors.initials: B
authors.lastname: Özyılmaz
authors.forename: M
authors.initials: M
authors.lastname: Tanrısev
authors.forename: G
authors.initials: G
authors.lastname: Afacan
authors.forename: H
authors.initials: H
authors.lastname: Colak
authors.forename: I
authors.initials: I
authors.lastname: Pirim
mesh_terms: D000328:Adult; D001323:Autoantibodies / Q000276:immunology*; D005260:Female; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D007676:Kidney Failure, Chronic / Q000276:immunology* / Q000601:surgery; D016030:Kidney Transplantation / Q000276:immunology*; D008297:Male; D008875:Middle Aged
chemical_list: D001323:Autoantibodies; D006680:HLA Antigens