title: Effect of fermented soybean product (Cheonggukjang) intake on metabolic parameters in mice fed a high-fat diet.
abstract: As a nontargeted metabolomics approach, we investigated changes in the plasma metabolite levels in a mouse model of obesity induced by a high-fat diet and fermented soybean product diet. We analyzed the plasma samples by using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). In the present study, the animals were divided into four groups according to the diet type; normal fat diet control group (ND), high-fat diet control group (HD), high-fat diet plus 30% cooked soybean power (HD + S), and high-fat diet plus 30% 72-h fermented Cheonggukjang powder (HD + CGJ). To examine the changes in plasma metabolite levels because of high-fat diet feeding, total cholesterol and triglyceride levels were measured. Total cholesterol and triglyceride levels were lower in the HD + S and HD + CGJ groups than in the ND group. According to partial least-squares discriminant analysis (PLS-DA), major metabolites contributing to the discrimination between each group were assigned as lipid metabolites in plasma, e.g., lyso-phosphatidylcholines and phosphatidylcholines. Therefore, diets containing soy-based food products, which are rich sources of isoflavonoids, might be helpful for controlling the lipid metabolism under high-fat diet conditions.
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, Seoul, Republic of Korea.
authors.forename: Jiyoung
authors.initials: J
authors.lastname: Kim
authors.forename: Jung Nam
authors.initials: JN
authors.lastname: Choi
authors.forename: Joo Hee
authors.initials: JH
authors.lastname: Choi
authors.forename: Youn Soo
authors.initials: YS
authors.lastname: Cha
authors.forename: Maria John
authors.initials: MJ
authors.lastname: Muthaiya
authors.forename: Choong Hwan
authors.initials: CH
authors.lastname: Lee
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000097:blood; D059305:Diet, High-Fat; D004195:Disease Models, Animal; D005285:Fermentation; D005511:Food Handling; D007529:Isoflavones / Q000494:pharmacology; D050356:Lipid Metabolism / Q000187:drug effects; D008297:Male; D013058:Mass Spectrometry; D055432:Metabolomics; D051379:Mice; D009765:Obesity / Q000097:blood / Q000178:diet therapy; D045730:Soy Foods; D014280:Triglycerides / Q000097:blood
chemical_list: D007529:Isoflavones; D014280:Triglycerides; D002784:Cholesterol
keywords: Cheonggukjang; Metabolite profiling; Partial least square-discriminate analysis; Plasma; Ultra-performance liquid chromatography (UPLC)