title: Development of an integrated optical analyzer for characterization of growth dynamics of bacterial colonies.
abstract: In order to understand the biophysics behind collective behavior of a bacterial colony, a confocal displacement meter was used to measure the profiles of the bacterial colonies, together with a custom built optical density circuits. The system delivered essential information related to the quantitative growth dynamics (height, diameter, aspect ratio, optical density) of the bacterial colony. For example, the aspect ratio of S. aureus was approximately two times higher than that of E. coli O157 : H7, while the OD of S. aureus was approximately 1/3 higher than that of E. coli O157 : H7.
authors.affiliation: School of Mechanical Engineering, 585 Purdue Mall, West Lafayette, IN 47907, USA.
authors.forename: Huisung
authors.initials: H
authors.lastname: Kim
authors.forename: Nan
authors.initials: N
authors.lastname: Bai
authors.forename: Arun K
authors.initials: AK
authors.lastname: Bhunia
authors.forename: Galen B
authors.initials: GB
authors.lastname: King
authors.forename: E Daniel
authors.initials: ED
authors.lastname: Hirleman
authors.forename: Euiwon
authors.initials: E
authors.lastname: Bae
mesh_terms: D019453:Escherichia coli O157 / Q000254:growth & development*; D007834:Lasers; D055096:Optical Devices; D013211:Staphylococcus aureus / Q000254:growth & development*; D013997:Time Factors
keywords: bacterial colony morphology; diffraction; instrumentation and measurement; light scattering