title: Caprine sex affects skeletal muscle profile and MRFs expression during postnatal development.
abstract: The important roles of myogenic regulatory factors (MRFs) have been well addressed in the process of mammalian skeletal myogenesis, while limited research has been performed in small ruminants. Furthermore, the effects of gender on the development of skeletal muscle and MRFs expression remain unknown. In this study, we identified the caprine Myf5, Myf6, MyoD and myogenin genes and quantified their expressions at six different postnatal time points by real-time RT-PCR. The sex has a marked effect on the expression differences of Myf5, MyoD and myogenin in the five investigated skeletal muscles, while minor influence on the expression difference of Myf6 except for Semitendinosus and Quadriceps femoris tissues (P < 0.001). The histological sections of muscles revealed a constant increase of muscle fiber diameter with aging but non-significant differences between genders. We provide novel evidence for MRFs expression in age- and gender-dependent manners, which will contribute to prioritizing these genes as potential candidate genes for trait-associated study and provide a foundation for understanding the molecular control of skeletal muscle growth in goat species.
authors.affiliation: Institute of Animal Genetics and Breeding, Sichuan Agricultural University, Ya'an, Sichuan, China.|College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhong
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan, China.
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Jin
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan, China.
authors.forename: En-Ni
authors.initials: EN
authors.lastname: Dong
authors.affiliation: Institute of Animal Genetics and Breeding, Sichuan Agricultural University, Ya'an, Sichuan, China.|College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Institute of Animal Genetics and Breeding, Sichuan Agricultural University, Ya'an, Sichuan, China.|College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan, China.
authors.forename: Lin-Jie
authors.initials: LJ
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan, China.
authors.forename: Hong-Ping
authors.initials: HP
authors.lastname: Zhang
mesh_terms: D000375:Aging / Q000502:physiology; D000818:Animals; D000831:Animals, Newborn; D005260:Female; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D008297:Male; D024510:Muscle Development / Q000235:genetics* / Q000502:physiology*; D018482:Muscle, Skeletal / Q000254:growth & development* / Q000378:metabolism*; D018006:Myogenic Regulatory Factors / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D018008:Myogenin / Q000235:genetics / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012727:Sex Characteristics; D012756:Sheep / Q000254:growth & development*
chemical_list: D018006:Myogenic Regulatory Factors; D018008:Myogenin