title: Enantiomeric analysis of limonene and carvone by direct introduction of aromatic plants into multidimensional gas chromatography.
abstract: Analysis of chiral compounds in complex mixtures is achieved by multidimensional gas chromatography using heptakis-(2,3,6-tri-O-methyl)-β-cyclodextrin stationary phase as the main column of the system to separate specific selected cuts containing components unresolved in the first dimension. The proposed procedure allows rapid analysis of both solid and liquid matrices by direct introduction, into the programmed temperature vaporizer (PTV) of a gas chromatograph, of either the plant material or the essential oil, respectively. A comparison between enantiomeric excesses data obtained, from plant leaves (or plant seeds) and the corresponding essential oils, by direct injection (i.e., without sample pretreatment or concentration step) into the multidimensional system is also included. Reported data demonstrate that no racemization occurs during analysis as identical enantiomeric excesses are obtained in both cases for specific chiral compounds.
authors.affiliation: Instituto de Ciencia y Tecnología de Alimentos y Nutrición, Consejo Superior de Investigaciones Científicas, c/Juan de Cierva 3, 28006 Madrid, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Barba
authors.forename: R M
authors.initials: RM
authors.lastname: Toledano
authors.forename: G
authors.initials: G
authors.lastname: Santa-María
authors.forename: M
authors.initials: M
authors.lastname: Herraiz
authors.forename: R M
authors.initials: RM
authors.lastname: Martínez
mesh_terms: D029974:Carum / Q000737:chemistry; D002849:Chromatography, Gas / Q000379:methods*; D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes / Q000302:isolation & purification*; D000077222:Limonene; D027541:Mentha / Q000737:chemistry; D039821:Monoterpenes / Q000302:isolation & purification*; D009822:Oils, Volatile / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D013237:Stereoisomerism; D013729:Terpenes / Q000302:isolation & purification*; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes; D039821:Monoterpenes; D009822:Oils, Volatile; D013729:Terpenes; D047392:beta-Cyclodextrins; C000609658:heptakis(2,3,6-tri-O-methyl)-beta-cyclodextrin; C006923:carvone; D000077222:Limonene