title: Evaluation of EGFR-targeted radioimmuno-gold-nanoparticles as a theranostic agent in a tumor animal model.
abstract: This study evaluated the tumor targeting and therapeutic efficacy of a novel theranostic agent (131)I-labeled immuno-gold-nanoparticle ((131)I-C225-AuNPs-PEG) for high epidermal growth factor receptor (EGFR)-expressed A549 human lung cancer. Confocal microscopy demonstrated the specific uptake of C225-AuNPs-PEG in A549 cells. (131)I-C225-AuNPs-PEG induced a significant reduction in cell viability, which was not observed when incubated with AuNPs-PEG and C225-AuNPs-PEG. MicroSPECT/CT imaging of tumor-bearing mice after intravenous injection of (123)I-C225-AuNPs-PEG revealed significant radioactivity retention in tumor suggested that (131)I-labeled C225-conjugated radioimmuno-gold-nanoparticles may provide a new approach of targeted imaging and therapy towards high EGFR-expressed cancers.
authors.affiliation: Department of Biomedical Imaging and Radiological Sciences, National Yang-Ming University, No. 155, Sec. 2, Linong St., Beitou District, Taipei 112, Taiwan, ROC.
authors.forename: Hao-Wen
authors.initials: HW
authors.lastname: Kao
authors.forename: Yi-Yu
authors.initials: YY
authors.lastname: Lin
authors.forename: Chao-Cheng
authors.initials: CC
authors.lastname: Chen
authors.forename: Kwan-Hwa
authors.initials: KH
authors.lastname: Chi
authors.forename: Der-Chi
authors.initials: DC
authors.lastname: Tien
authors.forename: Chien-Chung
authors.initials: CC
authors.lastname: Hsia
authors.forename: Ming-Hsien
authors.initials: MH
authors.lastname: Lin
authors.forename: Hsin-Ell
authors.initials: HE
authors.lastname: Wang
mesh_terms: D000818:Animals; D061067:Antibodies, Monoclonal, Humanized / Q000737:chemistry* / Q000627:therapeutic use / Q000633:toxicity; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000627:therapeutic use / Q000633:toxicity; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D000068818:Cetuximab; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D066246:ErbB Receptors / Q000037:antagonists & inhibitors* / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007275:Injections, Intravenous; D007457:Iodine Radioisotopes / Q000737:chemistry; D008175:Lung Neoplasms / Q000000981:diagnostic imaging / Q000188:drug therapy; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D018613:Microscopy, Confocal; D011092:Polyethylene Glycols / Q000737:chemistry; D019275:Radiopharmaceuticals / Q000737:chemistry* / Q000627:therapeutic use / Q000633:toxicity; D015899:Tomography, Emission-Computed, Single-Photon; D014057:Tomography, X-Ray Computed; D014183:Transplantation, Heterologous
chemical_list: D061067:Antibodies, Monoclonal, Humanized; D000970:Antineoplastic Agents; D007457:Iodine Radioisotopes; D019275:Radiopharmaceuticals; D011092:Polyethylene Glycols; D006046:Gold; D066246:ErbB Receptors; D000068818:Cetuximab