title: Profiling the jasmonic acid responses by nuclear magnetic resonance-based metabolomics.
abstract: Metabolomics based on nuclear magnetic resonance (NMR) can be used to monitor the metabolite response of plants to jasmonic acid. Metabolomics experiments consist of three important steps: sample preparation, NMR analysis, and data mining. In sample preparation, a very critical factor is the selection of a proper solvent for plant material extraction because the presence of metabolites will vary according to the solvent used. For NMR analysis, an intermediate-to-polar solvent, such as a mixture of methanol and water, is a good choice. In general, (1)H-NMR spectroscopy is the standard method for metabolite profiling, whereas two-dimensional spectroscopy provides more detailed information on the metabolites. Finally, various chemometric methods can be used for data mining. Here, we describe all three steps of metabolomic analysis by means of NMR spectroscopy.
authors.affiliation: Natural Product Laboratory, Institute of Biology, Leiden University, Leiden, The Netherlands.
authors.forename: Hye Kyong
authors.initials: HK
authors.lastname: Kim
authors.forename: Young Hae
authors.initials: YH
authors.lastname: Choi
authors.forename: Robert
authors.initials: R
authors.lastname: Verpoorte
mesh_terms: D018929:Cell Culture Techniques; D003517:Cyclopentanes / Q000494:pharmacology*; D057225:Data Mining; D020869:Gene Expression Profiling; D009682:Magnetic Resonance Spectroscopy; D055432:Metabolomics; D000432:Methanol / Q000737:chemistry; D054883:Oxylipins / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry; D010937:Plant Growth Regulators / Q000494:pharmacology / Q000502:physiology*; D052616:Solid Phase Extraction; D012997:Solvents / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D003517:Cyclopentanes; D054883:Oxylipins; D010936:Plant Extracts; D010937:Plant Growth Regulators; D012997:Solvents; D014867:Water; C011006:jasmonic acid; D000432:Methanol