title: [Determination of the content of the main components of flavonoids compounds in raw malt, torrefied malt and ustulate malt].
abstract: OBJECTIVE
  To determine the content of Catechin, Myricetin, Quercetin and Kaempferol in barley grain, raw malt, torrefied malt and ustulate malt based on different barley cultivars.
  
  
  METHODS
  HPLC method was used. Analysis was performed on Agilent ZORBAXSB-C18 (150 mm x 4. 6 mm, 3.5 microm) column with acetonitrile-0.1% acetic acid as mobile phase. The detection wavelength was 280 nm, flow rate was 0.8 mL/min, and the column temperature was 30 degrees C.
  
  
  RESULTS
  Catechin was the main component of barley seeds and its processed products. Slight reduction of catechin was observed in processed and sprouting seeds. Sprouting significantly increased the content of myricetin. Both barley seeds and the processed products were lack of quercetin. The amounts of kaempferol in seed were higher than that in barley grain, but similar to that in ustulate malt.
  
  
  CONCLUSIONS
  The content of flavonoids in raw malt and torrefied malt are significantly affected by sprouting and processing, and significance differences are presented among different varieties.
authors.affiliation: Chinese Pharmaceutical University, Nanjing 211198, China. hedanxia111@yahoo.com.cn
authors.forename: Dan-Xia
authors.initials: DX
authors.lastname: He
authors.forename: Liang
authors.initials: L
authors.lastname: Rong
authors.forename: Min-Jian
authors.initials: MJ
authors.lastname: Qin
authors.forename: Hui-Juan
authors.initials: HJ
authors.lastname: Liu
mesh_terms: D002392:Catechin / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis*; D001467:Hordeum / Q000737:chemistry* / Q000145:classification; D044949:Kaempferols / Q000032:analysis; D011794:Quercetin / Q000032:analysis; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry*; D013045:Species Specificity
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D044949:Kaempferols; C006552:kaempferol; C040015:myricetin; D002392:Catechin; D011794:Quercetin