title: Enhanced inhibition of Aspergillus niger on sedge (Lepironia articulata) treated with heat-cured lime oil.
abstract: OBJECTIVE
  This study aimed to examine heat curing effect (30-100°C) on antifungal activities of lime oil and its components (limonene, p-cymene, β-pinene and α-pinene) at concentrations ranging from 100 to 300 μl ml(-1) against Aspergillus niger in microbiological medium and to optimize heat curing of lime oil for efficient mould control on sedge (Lepironia articulata).
  
  
  RESULTS
  Broth dilution method was employed to determine lime oil minimum inhibitory concentration, which was at 90 μl ml(-1) with heat curing at 70°C. Limonene, a main component of lime oil, was an agent responsible for temperature dependencies of lime oil activities observed. Response surface methodology was used to construct the mathematical model describing a time period of zero mould growth on sedge as functions of heat curing temperature and lime oil concentration. Heat curing of 90 μl ml(-1) lime oil at 70°C extended a period of zero mould growth on sedge to 18 weeks under moist conditions.
  
  
  CONCLUSIONS
  Heat curing at 70°C best enhanced antifungal activity of lime oil against A. niger both in medium and on sedge.
  
  
  CONCLUSIONS
  Heat curing of lime oil has potential to be used to enhance the antifungal safety of sedge products.
authors.affiliation: Food Technology, School of Agricultural Technology, Walailak University, Nakon Si Thammarat, Thailand. nnarumol@wu.ac.th
authors.forename: N
authors.initials: N
authors.lastname: Matan
authors.forename: N
authors.initials: N
authors.lastname: Matan
authors.forename: S
authors.initials: S
authors.lastname: Ketsa
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D001234:Aspergillus niger / Q000187:drug effects*; D000081008:Bicyclic Monoterpenes; D001643:Bridged Bicyclo Compounds / Q000032:analysis; D032082:Citrus aurantiifolia / Q000737:chemistry*; D053138:Cyclohexenes / Q000032:analysis; D000081007:Cymenes; D029785:Cyperaceae / Q000382:microbiology*; D006358:Hot Temperature; D000077222:Limonene; D008826:Microbial Sensitivity Tests; D039821:Monoterpenes / Q000032:analysis; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology*; D013729:Terpenes / Q000032:analysis
chemical_list: D000935:Antifungal Agents; D000081008:Bicyclic Monoterpenes; D001643:Bridged Bicyclo Compounds; D053138:Cyclohexenes; D000081007:Cymenes; D039821:Monoterpenes; D010938:Plant Oils; D013729:Terpenes; C007210:4-cymene; C010789:beta-pinene; D000077222:Limonene; C005451:alpha-pinene
keywords: Aspergillus niger; heat curing; lime oil; medium; sedge (Lepironia articulata)