title: Gene expression study related with the intrinsic pathway of apoptosis in bladder cancer by real-time PCR technique.
abstract: We examined the expression of anti-apoptotic genes (XIAP and Bcl-2) and apoptotic genes (cytochrome c, caspase-9, Apaf-1) in tissue samples of patients with superficial bladder cancer. Thirty-two bladder cancer tissue samples (8 papillary urothelial neoplasm of low malignant potential, 10 low-grade, and 14 high-grade) and 8 normal bladder tissue samples from necropsy were used for the study of gene expression by real-time PCR analysis. Analysis of the expression of apoptotic gene constituents of an apoptosome demonstrated an increase in Apaf-1 expression in the three tumor grades when compared with the control (P < 0.01, P < 0.05, and P < 0.01), low expression of caspase-9 in all groups (P < 0.05), and an increase in cytochrome c expression in all tumor grades in relation to the control, although without statistically significant difference. The expression of anti-apoptotic genes revealed an increase in XIAP expression in all tumor grades in relation to the control, although without statistically significant difference, and low expression of Bcl-2 in all tumor grades and the control (P < 0.05). The results proved that there is low evidence of apoptotic activity by the intrinsic pathway, demonstrated by the low expression of caspase-9 and considerable increase in XIAP expression, which may render these genes potential therapeutic targets in bladder cancer treatment.
authors.affiliation: Departamento de Cirurgia e Anatomia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.
authors.forename: D F
authors.initials: DF
authors.lastname: Barione
authors.forename: F S N
authors.initials: FS
authors.lastname: Lizarte
authors.forename: P C
authors.initials: PC
authors.lastname: Novais
authors.forename: C A M
authors.initials: CA
authors.lastname: de Carvalho
authors.forename: F C B
authors.initials: FC
authors.lastname: Valeri
authors.forename: F M
authors.initials: FM
authors.lastname: Peria
authors.forename: H F
authors.initials: HF
authors.lastname: de Oliveira
authors.forename: D L
authors.initials: DL
authors.lastname: Zanette
authors.forename: W A
authors.initials: WA
authors.lastname: Silva
authors.forename: A J
authors.initials: AJ
authors.lastname: Cologna
authors.forename: R B
authors.initials: RB
authors.lastname: Reis
authors.forename: S
authors.initials: S
authors.lastname: Tucci
authors.forename: A C P
authors.initials: AC
authors.lastname: Martins
authors.forename: D P C
authors.initials: DP
authors.lastname: Tirapelli
authors.forename: L F
authors.initials: LF
authors.lastname: Tirapelli
mesh_terms: D017209:Apoptosis / Q000235:genetics*; D053477:Apoptotic Protease-Activating Factor 1 / Q000235:genetics / Q000378:metabolism; D053453:Caspase 9 / Q000235:genetics / Q000378:metabolism; D045304:Cytochromes c / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000378:metabolism*
chemical_list: D053477:Apoptotic Protease-Activating Factor 1; D019253:Proto-Oncogene Proteins c-bcl-2; D045304:Cytochromes c; D053453:Caspase 9