title: Pirimicarb-based formulation-induced genotoxicity and cytotoxicity in the freshwater fish Cnesterodon decemmaculatus (Jenyns, 1842) (Pisces, Poeciliidae).
abstract: We analyzed the aspects of lethality, genotoxicity, and cytotoxicity in the ten spotted live-bearer exposed under laboratory conditions to the pirimicarb-based formulation Patton Flow® (50% active ingredient (a.i.)). Acute effects were evaluated using different end points for lethality, genotoxicity, and cytotoxicity. Median lethal concentration (LC50) estimation was employed as a bioassay for lethality, whereas micronucleus (MN) induction and alterations in erythrocyte/erythroblast frequency were used as end points for genotoxicity and cytotoxicity, respectively. Results demonstrated an LC5096h value of 88 mg/L. Patton Flow® increased the MN frequency in fish erythrocytes after 48 h of exposure at a concentration of 66 mg/L, whereas a concentration range of 22-66 mg/L was able to exert the same genotoxic effect at 96 h of treatment. Furthermore, cytotoxicity was also observed by alterations in erythrocyte/erythroblast frequencies within the concentration range of 22-66 mg/L, regardless of the exposure time. Our current observations provide evidence that Patton Flow® (50% a.i.) should be considered a clear lethal, cytotoxic, and genotoxic agent on Cnesterodon decemmaculatus. Thus, repeated applications of this carbamic insecticide can enter the aquatic environment and exert deleterious effects on aquatic organisms other than the evaluated species C. decemmaculatus.
authors.affiliation: Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata Calle 64 No. 3, La Plata, Argentina Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
authors.forename: Josefina
authors.initials: J
authors.lastname: Vera-Candioti
authors.affiliation: Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata Calle 64 No. 3, La Plata, Argentina Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
authors.forename: Sonia
authors.initials: S
authors.lastname: Soloneski
authors.affiliation: Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata Calle 64 No. 3, La Plata, Argentina Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina marcelo.larramendy@gmail.com.
authors.forename: Marcelo L
authors.initials: ML
authors.lastname: Larramendy
mesh_terms: D000818:Animals; D002219:Carbamates / Q000633:toxicity*; D003532:Cyprinodontiformes; D004249:DNA Damage / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects; D005618:Fresh Water; D007306:Insecticides / Q000633:toxicity*; D007928:Lethal Dose 50; D015162:Micronucleus Tests; D011743:Pyrimidines / Q000633:toxicity*
chemical_list: D002219:Carbamates; D007306:Insecticides; D011743:Pyrimidines; C011994:pirimicarb
keywords: Patton Flow®; erythrocyte:erythroblast ratio; micronucleus; mortality; poeciliidae