title: Comparison of collagen matrix treatment impregnated with platelet rich plasma vs bone marrow.
abstract: This study has reported the efficacy of an autologous bone marrow-impregnated collagen matrix experimentally and clinically. Then, it reflected that platelet rich plasma (PRP) was as good a source of growth factors as bone marrow and available in a less invasive procedure. This study aimed to compare the efficacy of a PRP-impregnated collagen matrix with that of a bone marrow-impregnated collagen matrix by quantifying wound size and capillary density using genetically diabetic db/db mice. Bone marrow cells were obtained from femurs of ddy mice. Then, a small amount of collagen matrix was immersed in bone marrow suspension. This is called a bone marrow-impregnated collagen matrix. PRP was obtained from healthy human blood and a small amount of collagen matrix was immersed in PRP. This is called a PRP-impregnated collagen matrix. A bone marrow-impregnated collagen matrix and PRP-impregnated collagen matrix were applied to excisional skin wounds on a genetically healing-impaired mouse (n = 6) and wounds were evaluated 6 days after the procedure. Wounds were divided into two groups: PRP (n = 6), in which a PRP-impregnated collagen matrix was applied; and bone marrow (n = 6), in which collagen immersed in a bone marrow suspension was applied. There was no significant difference between the PRP and bone-marrow groups in the rate of vascular density increase or wound size decrease. The present study suggested that the PRP-impregnated collagen matrix promotes repair processes at least as strongly as the bone marrow-impregnated collagen matrix. Given lower invasiveness, the PRP-impregnated collagen matrix would have advantages in clinical use.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Saitama Medical University Hospital , Saitama , Japan.
authors.forename: Ai
authors.initials: A
authors.lastname: Minamimura
authors.forename: Shigeru
authors.initials: S
authors.lastname: Ichioka
authors.forename: Hitomi
authors.initials: H
authors.lastname: Sano
authors.forename: Naomi
authors.initials: N
authors.lastname: Sekiya
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods*; D003094:Collagen / Q000008:administration & dosage*; D017719:Diabetic Foot / Q000503:physiopathology / Q000601:surgery*; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D008833:Microcirculation / Q000502:physiology; D008875:Middle Aged; D018919:Neovascularization, Physiologic / Q000187:drug effects / Q000502:physiology; D053657:Platelet-Rich Plasma; D054457:Tissue Scaffolds; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology*
chemical_list: D003094:Collagen