title: Green coffee oil analysis by high-resolution nuclear magnetic resonance spectroscopy.
abstract: In this work, we show how an extensive and fast quantification of the main components in green coffee oil can be achieved by NMR, with minimal sample manipulation and use of organic solvents. The approach is based on the integration of characteristic NMR signals, selected because of their similar relaxation properties and because they fall in similar spectral regions, which minimizes offset effects. Quantification of glycerides, together with their fatty acid components (oleic, linoleic, linolenic and saturated) and minor species (caffeine, cafestol, kahweol and 16-O-methylcafestol), is achieved in less than 1h making use of (1)H and (13)C spectroscopy. The compositional data obtained are in reasonable agreement with classical chromatographic analyses.
authors.affiliation: Bracco Imaging-CRB Trieste Area Science Park, Basovizza, Trieste, Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: D'Amelio
authors.forename: Elisabetta
authors.initials: E
authors.lastname: De Angelis
authors.forename: Luciano
authors.initials: L
authors.lastname: Navarini
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Schievano
authors.forename: Stefano
authors.initials: S
authors.lastname: Mammi
mesh_terms: D002849:Chromatography, Gas; D003069:Coffee / Q000737:chemistry*; D005227:Fatty Acids / Q000032:analysis; D005989:Glycerides / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*
chemical_list: D003069:Coffee; D005227:Fatty Acids; D005989:Glycerides