title: Ultrasensitive and selective gold film-based detection of mercury (II) in tap water using a laser scanning confocal imaging-surface plasmon resonance system in real time.
abstract: An ultrasensitive and selective detection of mercury (II) was investigated using a laser scanning confocal imaging-surface plasmon resonance system (LSCI-SPR). The detection limit was as low as 0.01ng/ml for Hg(2+) ions in ultrapure and tap water based on a T-rich, single-stranded DNA (ssDNA)-modified gold film, which can be individually manipulated using specific T-Hg(2+)-T complex formation. The quenching intensity of the fluorescence images for rhodamine-labeled ssDNA fitted well with the changes in SPR. The changes varied with the Hg(2+) ion concentration, which is unaffected by the presence of other metal ions. The coefficients obtained for ultrapure and tap water were 0.99902 and 0.99512, respectively, for the linear part over a range of 0.01-100ng/ml. The results show that the double-effect sensor has potential for practical applications with ultra sensitivity and selectivity, especially in online or real-time monitoring of Hg(2+) ions pollution in tap water with the further improvement of portable LSCI-SPR instrument.
authors.affiliation: Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zhang
authors.forename: Liquan
authors.initials: L
authors.lastname: Yang
authors.forename: Bingjiang
authors.initials: B
authors.lastname: Zhou
authors.forename: Weimin
authors.initials: W
authors.lastname: Liu
authors.forename: Jiechao
authors.initials: J
authors.lastname: Ge
authors.forename: Jiasheng
authors.initials: J
authors.lastname: Wu
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Pengfei
authors.initials: P
authors.lastname: Wang
mesh_terms: D060766:Drinking Water / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D008628:Mercury / Q000302:isolation & purification*; D053768:Metal Nanoparticles / Q000737:chemistry; D018613:Microscopy, Confocal; D020349:Surface Plasmon Resonance; D014874:Water Pollutants, Chemical / Q000302:isolation & purification*
chemical_list: D060766:Drinking Water; D014874:Water Pollutants, Chemical; D006046:Gold; D008628:Mercury