title: Multidimensional protein identification technology for direct-tissue proteomics of heart.
abstract: Multidimensional protein identification technology (MudPIT) is an invaluable approach to identify proteins at large-scale level. Here, we describe a procedure of investigation to functional characterize the proteomic profile of complex samples such as those from cardiac tissues. In particular, we focus on the main steps concerning sample preparation, MudPIT analysis, tandem mass spectra processing, and biomarker discovery using label-free approaches. Finally, we report a data-derived systems biology approach to identify groups of proteins of over-, under-, and normal expression.
authors.affiliation: Proteomics and Metabolomics Unit, Institute for Biomedical Technologies, CNR, Milan, Italy.
authors.forename: Dario
authors.initials: D
authors.lastname: Di Silvestre
authors.forename: Francesca
authors.initials: F
authors.lastname: Brambilla
authors.forename: Pier Luigi
authors.initials: PL
authors.lastname: Mauri
mesh_terms: D000465:Algorithms; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002853:Chromatography, Liquid; D030562:Databases, Protein; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D009206:Myocardium / Q000378:metabolism*; D060066:Protein Interaction Maps; D059748:Proteolysis; D020543:Proteome / Q000737:chemistry* / Q000302:isolation & purification; D012984:Software; D049490:Systems Biology; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D015415:Biomarkers; D020543:Proteome