title: Exploiting the mechanism of cellular glucose uptake to develop an image-based high-throughput screening system in living cells.
abstract: Biophysical understanding of cellular glucose uptake led us to the development of an image-based high-throughput screening system by using a fluorescent glucose bioprobe, GB2. The accuracy, robustness, and practicality of our image-based HTS system were demonstrated through the pilot screening and the subsequent in vitro confirmation.
authors.affiliation: Department of Chemistry, Seoul National University, Seoul, Korea.
authors.forename: Ala
authors.initials: A
authors.lastname: Jo
authors.forename: Jongmin
authors.initials: J
authors.lastname: Park
authors.forename: Seung Bum
authors.initials: SB
authors.lastname: Park
mesh_terms: D000818:Animals; D001692:Biological Transport; D015374:Biosensing Techniques / Q000379:methods; D002460:Cell Line; D002470:Cell Survival; D005456:Fluorescent Dyes / Q000032:analysis* / Q000378:metabolism; D005947:Glucose / Q000032:analysis / Q000378:metabolism*; D057166:High-Throughput Screening Assays / Q000379:methods*; D051379:Mice; D032446:Myoblasts / Q000378:metabolism; D061848:Optical Imaging / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D005947:Glucose