title: Automated annotation and quantification of glycans using liquid chromatography-mass spectrometry.
abstract: As a common post-translational modification, protein glycosylation plays an important role in many biological processes, and it is known to be associated with human diseases. Mass spectrometry (MS)-based glycomic profiling techniques have been developed to measure the abundances of glycans in complex biological samples and applied to the discovery of putative glycan biomarkers. To automate the annotation of glycomic profiles in the liquid chromatography-MS (LC-MS) data, we present here a user-friendly software tool, MultiGlycan, implemented in C# on Windows systems. We tested MultiGlycan by using several glycomic profiling datasets acquired using LC-MS under different preparations and show that MultiGlycan executes fast and generates robust and reliable results.
  
  
  BACKGROUND
  MultiGlycan can be freely downloaded at http://darwin.informatics.indiana.edu/MultiGlycan/.
  
  
  BACKGROUND
  Supplementary data are available at Bioinformatics online.
authors.affiliation: School of Informatics and Computing, Indiana University, Bloomington, IN 47408, USA. chuyu@indiana.edu
authors.forename: Chuan-Yih
authors.initials: CY
authors.lastname: Yu
authors.forename: Anoop
authors.initials: A
authors.lastname: Mayampurath
authors.forename: Yunli
authors.initials: Y
authors.lastname: Hu
authors.forename: Shiyue
authors.initials: S
authors.lastname: Zhou
authors.forename: Yehia
authors.initials: Y
authors.lastname: Mechref
authors.forename: Haixu
authors.initials: H
authors.lastname: Tang
mesh_terms: D045744:Cell Line, Tumor; D002853:Chromatography, Liquid / Q000379:methods*; D054794:Glycomics / Q000379:methods; D006023:Glycoproteins / Q000737:chemistry*; D006031:Glycosylation; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D058977:Molecular Sequence Annotation; D011134:Polysaccharides / Q000032:analysis* / Q000737:chemistry; D011499:Protein Processing, Post-Translational; D012984:Software
chemical_list: D006023:Glycoproteins; D011134:Polysaccharides