title: Iron bioavailability of different maize genotypes developed in a breeding program: in vitro and in vivo studies.
abstract: The objective of this study was to evaluate iron bioavailability of maize genotypes, and analyze the correlation between in vitro and in vivo methods. Dialysable iron was analyzed in 13 genotypes from which 5 were selected for the biological assay. Mean iron content of the genotypes (n = 13) was 17.93 +/- 2.93 mg kg(-1). Phytate varied from 0.77% to 1.03%; phytate: iron molar ratio from 30.64 to 55.41; and soluble iron from 13.17 to 39.63%. The highest value for dialysable iron was 19.14%. In the biological assay, the control group, that received ferrous sulphate, did not present significant difference between the genotypes for Hb gain, Hb gain per gram of iron consumed and HRE. Hb gain did not present a significant correlation with in vitro assay. However, there were positive correlations varying from 0.653 to 0.809. The maize genotypes evaluated presented a good bioavailability since the genotypes showed the same result in hemoglobin gain than control group.
authors.affiliation: Federal University of Viçosa, Viçosa, Minas Gerais, Brazil.
authors.forename: Vânia Mayumi
authors.initials: VM
authors.lastname: Nakajima
authors.forename: Neuza Maria Brunoro
authors.initials: NM
authors.lastname: Costa
authors.forename: Hércia Stampini Duarte
authors.initials: HS
authors.lastname: Martino
authors.forename: Valéria Aparecida Vieira
authors.initials: VA
authors.lastname: Queiroz
authors.forename: Paulo Evaristo de Oliveira
authors.initials: PE
authors.lastname: Guimarães
authors.forename: Pedro Vitoriano
authors.initials: PV
authors.lastname: Oliveira
mesh_terms: D018798:Anemia, Iron-Deficiency / Q000178:diet therapy / Q000517:prevention & control*; D000818:Animals; D001681:Biological Assay / Q000379:methods; D001682:Biological Availability; D001947:Breeding; D003956:Dialysis; D005527:Food, Fortified / Q000032:analysis*; D005838:Genotype; D006454:Hemoglobins / Q000378:metabolism; D019266:Iron, Dietary / Q000032:analysis / Q000493:pharmacokinetics*; D008297:Male; D010833:Phytic Acid / Q000032:analysis*; D030821:Plants, Genetically Modified / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D013054:Spectrophotometry, Atomic; D003313:Zea mays / Q000737:chemistry / Q000235:genetics*
chemical_list: D006454:Hemoglobins; D019266:Iron, Dietary; D010833:Phytic Acid