title: The manner in which DNA is packaged with TFAM has an impact on transcription activation and inhibition.
abstract: For successful mitochondrial transgene expression, an optimal packaging exogenous DNA is an important issue. We report herein on the effects of DNA packaged with mitochondrial transcription factor A (TFAM), which packages mitochondrial DNA (mtDNA), on the transcription process. Our initial findings indicated that the transcription of the TFAM/DNA complex was activated, when the complex was formed at an optimal ratio. We also found that TFAM has a significant advantage over protamine, a nuclear DNA packaging protein, from the viewpoint of transcription efficiency. This result indicates that TFAM can be useful packaging protein for exogenous DNA to achieve mitochondrial transgene expression.
authors: Laboratory for Molecular Design of Pharmaceutics, Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan.
authors: Ryo
authors: R
authors: Furukawa
authors: Yuma
authors: Y
authors: Yamada
authors: Yuichi
authors: Y
authors: Matsushima
authors: Yu-Ichi
authors: Y
authors: Goto
authors: Hideyoshi
authors: H
authors: Harashima
keywords: DNA packaging; Mitochondria; Mitochondrial DNA (mtDNA); Mitochondrial transcription; Mitochondrial transcription factor A (TFAM)
references: J Mol Biol. 1995 May 26;249(1):11-28
references: 7776365
references: Mol Cell Biol. 1988 Aug;8(8):3496-509
references: 3211148
references: Mol Ther. 2011 Aug;19(8):1449-56
references: 21694702
references: Nature. 1978 Jan 12;271(5641):130-5
references: 622153
references: Nucleic Acids Res. 2002 May 1;30(9):1929-34
references: 11972329
references: Int J Biochem Cell Biol. 2009 Oct;41(10):1899-906
references: 19703654
references: Biomaterials. 2012 Feb;33(5):1589-95
references: 22105068
references: Annu Rev Genet. 2005;39:359-407
references: 16285865
references: Mitochondrion. 2007 Sep;7(5):311-21
references: 17698423
references: Nat Struct Mol Biol. 2011 Oct 30;18(11):1281-9
references: 22037172
references: Trends Genet. 2006 Feb;22(2):90-5
references: 16364493
references: Mol Biol Cell. 2007 Sep;18(9):3225-36
references: 17581862
references: Biochem Biophys Res Commun. 2010 Jun 25;397(2):181-6
references: 20580633
references: Proc Natl Acad Sci U S A. 2010 Oct 26;107(43):18410-5
references: 20930118
references: Eur J Biochem. 1996 Nov 1;241(3):779-86
references: 8944766
references: Proc Natl Acad Sci U S A. 2011 Aug 16;108(33):13534-9
references: 21808029
references: Gene Ther. 1997 Sep;4(9):961-8
references: 9349433
references: Nucleic Acids Res. 2009 Jun;37(10):3153-64
references: 19304746
references: Biochim Biophys Acta. 2008 Feb;1778(2):423-32
references: 18054323
references: Nucleic Acids Res. 2006;34(20):5815-28
references: 17062618
references: Ann N Y Acad Sci. 2004 Apr;1011:61-8
references: 15126284
references: Gene Ther. 1998 Jul;5(7):930-7
references: 9813664
references: Genes Cells. 2009 Aug;14(8):1029-42
references: 19624753
references: Physiol Rev. 2008 Apr;88(2):611-38
references: 18391175
references: J Gene Med. 2009 Jul;11(7):615-23
references: 19396931
references: Annu Rev Genet. 2011;45:299-329
references: 21910628
references: J Biol Chem. 2008 Feb 8;283(6):3665-3675
references: 18063578
references: BMC Biol. 2004 May 24;2:9
references: 15157274
references: Biomaterials. 2012 May;33(15):3952-8
references: 22386920
references: Mitochondrion. 2007 Feb-Apr;7(1-2):39-44
references: 17280879
references: Curr Opin Biotechnol. 2010 Oct;21(5):640-5
references: 20674331
references: J Biol Chem. 2009 May 15;284(20):13641-13647
references: 19307179
references: J Biol Chem. 1992 Feb 15;267(5):3358-67
references: 1737790