title: Rapid molecular detection of macrolide resistance in the Mycobacterium avium complex: are we there yet?
abstract: Macrolides are an important tool in the treatment of Mycobacterium avium complex infections. Here, we evaluate the use of 23S rRNA gene sequencing for the rapid detection of macrolide resistance. Routine sequencing of the 23S rRNA gene is highly specific for macrolide resistance but lacks in sensitivity.
authors: National Reference Centre for Mycobacteriology, National Microbiology Laboratory Public Health Agency of Canada, Winnipeg, Manitoba, Canada. sara.christianson@phac-aspc.gc.ca
authors: Sara
authors: S
authors: Christianson
authors: William
authors: W
authors: Grierson
authors: Joyce
authors: J
authors: Wolfe
authors: Meenu K
authors: MK
authors: Sharma
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D024881:Drug Resistance, Bacterial; D006801:Humans; D018942:Macrolides / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000379:methods; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D015269:Mycobacterium avium Complex / Q000187:drug effects* / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D018942:Macrolides; D012338:RNA, Ribosomal, 23S
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