title: Role of KCa3.1 channels in proliferation and migration of vascular smooth muscle cells by diabetic rat serum.
abstract: Proliferation and migration of vascular smooth muscle cells (VSMCs) are important events in the development of diabetic atherosclerosis. Previous studies have suggested that K(Ca)3.1 channels participate in atherosclerosis and coronary artery restenosis. In the present study, we attempted to clarify the roles of K(Ca)3.1 channels in the proliferation and migration of VSMCs using experimental type-2 diabetes rat serum and aortic smooth muscle cells (SMC) prepared from non-diabetic rats. mRNA and protein levels and current density of K(Ca)3.1 channels were greatly enhanced in cultured VSMCs treated with diabetic serum. In addition, diabetic serum promoted cell proliferation and migration in cultured VSMCs, and the effects were fully reversed in the cells treated with the K(Ca)3.1 channels blocker TRAM-34. In conclusion, serum from diabetic rats increases the expression of K(Ca)3.1 channels and promotes proliferation and migration of VSMCs to possibly participate in vascular remodeling in diabetes.
authors: Department of Pathophysiology, Xi'an Medical University, Xi'an, 710021, Shaanxi, People's Republic of China. suxingli@126.com
authors: Xing-Li
authors: XL
authors: Su
authors: Department of Infectious Disease, Shaanxi Provincial People's Hospital, Xi'an 710068, Shaanxi, People's Republic of China.
authors: Hong
authors: H
authors: Zhang
authors: Department of Pathophysiology, Xi'an Medical University, Xi'an, 710021, Shaanxi, People's Republic of China.
authors: Wei
authors: W
authors: Yu
authors: Department of Pathophysiology, Xi'an Medical University, Xi'an, 710021, Shaanxi, People's Republic of China.
authors: Shuang
authors: S
authors: Wang
authors: Department of Cardiology, Xi'an GaoXin Hospital, Xi'an, 710075, Shaanxi, People's Republic of China.
authors: Wei-Jun
authors: WJ
authors: Zhu
mesh_terms: D000818:Animals; D050197:Atherosclerosis / Q000097:blood / Q000209:etiology* / Q000473:pathology; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000473:pathology*; D003925:Diabetic Angiopathies / Q000097:blood / Q000209:etiology* / Q000473:pathology; D051660:Intermediate-Conductance Calcium-Activated Potassium Channels / Q000378:metabolism*; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D032389:Myocytes, Smooth Muscle / Q000502:physiology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D044967:Serum / Q000502:physiology
chemical_list: D051660:Intermediate-Conductance Calcium-Activated Potassium Channels; C491575:Kcnn4 protein, rat