title: Magneto-controllable capture and release of cancer cells by using a micropillar device decorated with graphite oxide-coated magnetic nanoparticles.
abstract: Aiming to highly efficient capture and analysis of circulating tumor cells, a micropillar device decorated with graphite oxide-coated magnetic nanoparticles is developed for magneto-controllable capture and release of cancer cells. Graphite oxide-coated, Fe3 O4 magnetic nanoparticles (MNPs) are synthesized by solution mixing and functionalized with a specific antibody, following by the immobilization of such modified MNPs on our designed micropillar device. For the proof-of-concept study, a HCT116 colorectal cancer cell line is employed to exam the capture efficiency. Under magnetic field manipulation, the high density packing of antibody-modified MNPs on the micropillars increases the local concentration of antibody, as well as the topographic interactions between cancer cells and micropillar surfaces. The flow rate and the micropillar geometry are optimized by studying their effects on capture efficiency. Then, a different number of HCT116 cells spiked in two kinds of cell suspension are investigated, yielding capture efficiency >70% in culture medium and >40% in blood sample, respectively. Moreover, the captured HCT116 cells are able to be released from the micropillars with a saturated efficiency of 92.9% upon the removal of applied magnetic field and it is found that 78% of the released cancer cells are viable, making them suitable for subsequent biological analysis.
authors: Key Laboratory of Artificial Micro- and Nano-Structures of the Ministry of Education, School of Physics and Technology, Wuhan University, Wuhan 430072, Hubei, China.
authors: Xiaolei
authors: X
authors: Yu
authors: Rongxiang
authors: R
authors: He
authors: Shasha
authors: S
authors: Li
authors: Bo
authors: B
authors: Cai
authors: Libo
authors: L
authors: Zhao
authors: Lei
authors: L
authors: Liao
authors: Wei
authors: W
authors: Liu
authors: Qian
authors: Q
authors: Zeng
authors: Hao
authors: H
authors: Wang
authors: Shi-Shang
authors: SS
authors: Guo
authors: Xing-Zhong
authors: XZ
authors: Zhao
mesh_terms: D002469:Cell Separation / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D045325:HCT116 Cells; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*
chemical_list: D058185:Magnetite Nanoparticles; D006108:Graphite
keywords: cancer cells; graphite oxide; magnetic nanoparticles; micropillars