title: Ser/Thr kinase-like protein of Nicotiana benthamiana is involved in the cell-to-cell movement of Bamboo mosaic virus.
abstract: To investigate the plant genes affected by Bamboo mosaic virus (BaMV) infection, we applied a cDNA-amplified fragment length polymorphism technique to screen genes with differential expression. A serine/threonine kinase-like (NbSTKL) gene of Nicotiana benthamiana is upregulated after BaMV infection. NbSTKL contains the homologous domain of Ser/Thr kinase. Knocking down the expression of NbSTKL by virus-induced gene silencing reduced the accumulation of BaMV in the inoculated leaves but not in the protoplasts. The spread of GFP-expressing BaMV in the inoculated leaves is also impeded by a reduced expression of NbSTKL. These data imply that NbSTKL facilitates the cell-to-cell movement of BaMV. The subcellular localization of NbSTKL is mainly on the cell membrane, which has been confirmed by mutagenesis and fractionation experiments. Combined with the results showing that active site mutation of NbSTKL does not change its subcellular localization but significantly affects BaMV accumulation, we conclude that NbSTKL may regulate BaMV movement on the cell membrane by its kinase-like activity. Moreover, the transient expression of NbSTKL does not significantly affect the accumulation of Cucumber mosaic virus (CMV) and Potato virus X (PVX); thus, NbSTKL might be a specific protein facilitating BaMV movement.
authors: Graduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan.
authors: Shun-Fang
authors: SF
authors: Cheng
authors: Meng-Shan
authors: MS
authors: Tsai
authors: Chia-Lin
authors: CL
authors: Huang
authors: Ying-Ping
authors: YP
authors: Huang
authors: I-Hsuan
authors: IH
authors: Chen
authors: Na-Sheng
authors: NS
authors: Lin
authors: Yau-Heiu
authors: YH
authors: Hsu
authors: Ching-Hsiu
authors: CH
authors: Tsai
authors: Chi-Ping
authors: CP
authors: Cheng
mesh_terms: D020134:Catalytic Domain / Q000235:genetics; D018076:DNA, Complementary; D018506:Gene Expression Regulation, Plant; D055785:Gene Knockdown Techniques; D054884:Host-Pathogen Interactions; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D010766:Phosphorylation; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism / Q000821:virology; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017863:Potexvirus / Q000502:physiology*; D011494:Protein Kinases / Q000378:metabolism; D021381:Protein Transport; D011523:Protoplasts / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism* / Q000821:virology*; D014779:Virus Replication
chemical_list: D018076:DNA, Complementary; D008565:Membrane Proteins; D010940:Plant Proteins; D011494:Protein Kinases
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