title: No detection of the retrovirus xenotropic murine leukemia virus-related virus in individuals with hemophilia.
abstract: BACKGROUND
  Xenotropic murine leukemia virus-related virus (XMRV) is a retrovirus that has recently been related to prostate cancers and chronic fatigue syndrome. Since other human-pathogenic retroviruses, such as HIV, human T-lymphotropic virus type I (HTLV-I) and -II, are known blood-transmitted pathogens, XMRV might present another hazard associated with products derived from in vitro cultures of human or animal origin, or blood component-based therapeutics. Here, we investigated whether XMRV was transmitted to individuals with hemophilia and frequent exposure to plasma-derived or recombinant clotting factors.
  
  
  METHODS
  We used highly sensitive real-time PCR to test plasma samples from 127 consecutive individuals with hemophilia who consulted our hemophilia center either for treatment or for a standard check-up.
  
  
  RESULTS
  From the 127 hemophiliacs, 80 had prior contact to persons with either hepatitis B (n = 30), hepatitis C (n = 74) and/or HIV (n = 21), and 30 were currently being treated with plasma-derived and 97 with recombinant factor concentrates. None of the individuals tested positive for XMRV.
  
  
  CONCLUSIONS
  Independent of the ongoing discussion on whether the positive XMRV testing in initial reports was a result of reagent, sample, or tissue contamination, and whether XMRV is a real threat or a testing artifact, our data suggest that XMRV might not play an important role for hemophiliacs.
authors: Institut für Transfusionsmedizin und Immunhämatologie, Klinikum der Johann Wolfgang Goethe Universität, DRK-Blutspendedienst Baden-Württemberg - Hessen, Frankfurt/M., Germany ; Chemotherapeutisches Forschungsinstitut Georg-Speyer-Haus, Frankfurt/M., Germany.
authors: Jörg
authors: J
authors: Schüttrumpf
authors: M Kai
authors: MK
authors: Hourfar
authors: Sonja
authors: S
authors: Alesci
authors: Wolfgang
authors: W
authors: Miesbach
authors: Erhard
authors: E
authors: Seifried
authors: Michael
authors: M
authors: Schmidt
keywords: Hemophilia; Plasma products; Virus contamination; Virus safety; XMRV
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