title: High-resolution analysis of cis-acting regulatory networks at the α-globin locus.
abstract: We have combined the circular chromosome conformation capture protocol with high-throughput, genome-wide sequence analysis to characterize the cis-acting regulatory network at a single locus. In contrast to methods which identify large interacting regions (10-1000 kb), the 4C approach provides a comprehensive, high-resolution analysis of a specific locus with the aim of defining, in detail, the cis-regulatory elements controlling a single gene or gene cluster. Using the human α-globin locus as a model, we detected all known local and long-range interactions with this gene cluster. In addition, we identified two interactions with genes located 300 kb (NME4) and 625 kb (FAM173a) from the α-globin cluster.
authors: MRC Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, Oxford University, Oxford OX3 9DS, UK.
authors: Jim R
authors: JR
authors: Hughes
authors: Karen M
authors: KM
authors: Lower
authors: Ian
authors: I
authors: Dunham
authors: Stephen
authors: S
authors: Taylor
authors: Marco
authors: M
authors: De Gobbi
authors: Jacqueline A
authors: JA
authors: Sloane-Stanley
authors: Simon
authors: S
authors: McGowan
authors: Jiannis
authors: J
authors: Ragoussis
authors: Douglas
authors: D
authors: Vernimmen
authors: Richard J
authors: RJ
authors: Gibbons
authors: Douglas R
authors: DR
authors: Higgs
mesh_terms: D000076246:CCCTC-Binding Factor; D042002:Chromatin Assembly and Disassembly; D002885:Chromosomes, Human, Pair 16 / Q000235:genetics / Q000378:metabolism; D053263:Gene Regulatory Networks; D056426:Genetic Loci; D015894:Genome, Human; D006801:Humans; D016366:Open Reading Frames; D011401:Promoter Regions, Genetic; D025941:Protein Interaction Mapping; D012045:Regulatory Sequences, Nucleic Acid; D012097:Repressor Proteins / Q000235:genetics; D055542:alpha-Globins / Q000235:genetics / Q000378:metabolism*; D055544:beta-Globins / Q000378:metabolism
chemical_list: D000076246:CCCTC-Binding Factor; C000619697:CTCF protein, human; D012097:Repressor Proteins; D055542:alpha-Globins; D055544:beta-Globins
keywords: chromatin conformation capture; cis-regulatory elements; α-globin locus
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