title: Quorum sensing signal molecules produced by Pseudomonas aeruginosa cause inflammation and escape host factors in murine model of urinary tract infection.
abstract: Quorum sensing (QS) is well established for its role in pathogenesis of various infections of Pseudomonas aeruginosa. However, its role in local tissue damage during urinary tract infection (UTI) is not yet fully established. To have insight in this, the present study was planned. UTI was established in mice using standard strain PAO1 and its isogenic QS mutant JP2. One group was challenged only with QS signals. Damage was assessed in terms of histopathology and pathology markers, malondialdehyde (MDA) and reactive nitrogen intermediates (RNI). Effect on pathogen motility, uroepithelial adhesion, and host serum sensitivity was also ascertained. PAO1-infected mice showed severe inflammation and tissue destruction, while mice infected with JP2 showed no significant destruction. JP2 was also unable to mount any tissue pathology markers, MDA and RNI, whereas PAO1 showed significantly higher levels of these two. Presence of only QS signals also showed considerable renal pathology. Strain JP2 also showed less motility, reduced uroepithelial cell adhesion, and increased serum sensitivity. Result highlights that QS signals induce local tissue pathology along with interference of host protective mechanisms during UTI.
authors: Department of Microbiology, BMS Block, Panjab University, Chandigarh, India, 160014.
authors: Parul
authors: P
authors: Gupta
authors: Ravi Kumar
authors: RK
authors: Gupta
authors: Kusum
authors: K
authors: Harjai
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D005260:Female; D007249:Inflammation / Q000276:immunology / Q000382:microbiology; D008315:Malondialdehyde / Q000378:metabolism*; D051379:Mice; D011552:Pseudomonas Infections / Q000378:metabolism / Q000473:pathology; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism; D011704:Pyelonephritis / Q000382:microbiology / Q000473:pathology*; D053038:Quorum Sensing / Q000235:genetics*; D026361:Reactive Nitrogen Species / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics; D014551:Urinary Tract / Q000382:microbiology / Q000473:pathology; D014552:Urinary Tract Infections / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology
chemical_list: D026361:Reactive Nitrogen Species; D008315:Malondialdehyde
references: J Burn Care Rehabil. 1996 Sep-Oct;17(5):384-9
references: 8889860
references: J Infect Dis. 1987 Sep;156(3):490-4
references: 3302054
references: J Surg Res. 2004 Jan;116(1):137-44
references: 14732360
references: Clin Sci (Lond). 2008 Dec;115(11):343-51
references: 18363571
references: Semin Pediatr Surg. 1995 May;4(2):77-82
references: 7633853
references: J Med Microbiol. 2002 Dec;51(12):1063-1070
references: 12466404
references: J Bacteriol. 2000 Nov;182(21):5990-6
references: 11029417
references: Mol Microbiol. 2003 Oct;50(1):61-8
references: 14507363
references: J Bacteriol. 1999 Mar;181(5):1623-9
references: 10049396
references: PLoS One. 2011 Jan 31;6(1):e16246
references: 21305014
references: Intensive Care Med. 2003 Jun;29(6):929-932
references: 12684747
references: J Microbiol Immunol Infect. 2006 Aug;39(4):302-9
references: 16926976
references: Mol Microbiol. 1998 Oct;30(2):295-304
references: 9791175
references: Cytokine. 2001 Nov 21;16(4):160-8
references: 11792126
references: Lung. 2000;178(3):181-9
references: 10871436
references: J Bacteriol. 2002 Feb;184(4):1132-9
references: 11807074
references: Infect Immun. 1996 Jan;64(1):37-43
references: 8557368
references: Infect Immun. 1999 Nov;67(11):5854-62
references: 10531240
references: Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4885-90
references: 10758151
references: Science. 1998 Apr 10;280(5361):295-8
references: 9535661
references: Infect Immun. 1998 Jul;66(7):3242-9
references: 9632591
references: Microbiology (Reading). 2009 Aug;155(Pt 8):2612-2619
references: 19460822
references: World J Surg. 1992 Jan-Feb;16(1):30-6
references: 1290263
references: Kidney Int. 2009 Aug;76(3):286-92
references: 19494801
references: Microbiol Rev. 1983 Mar;47(1):46-83
references: 6343827
references: J Med Microbiol. 2005 Jun;54(Pt 6):515-518
references: 15888457
references: Am J Respir Crit Care Med. 2003 Jun 1;167(11):1478-82
references: 12569080