title: Biophysical studies on the interactions of jatrorrhizine with bovine serum albumin by spectroscopic and molecular modeling methods.
abstract: The interaction between jatrorrhizine (JAT) and bovine serum albumin (BSA) has been studied. The studies were carried out in a buffer medium at pH 7.4 using fluorescence spectroscopy, UV-vis spectroscopy, and molecular modeling methods. The results of fluorescence quenching and UV-vis absorption spectra experiments indicated the formation of the complex of BSA-JAT. Binding parameters were determined using the Stern-Volmer equation and Scatchard equation. The results of thermodynamic parameters ΔG, ΔH and ΔS at different temperatures indicate that the electrostatic interactions and hydrogen bonds play a major role for JAT-BSA association. Site marker competitive displacement experiments and molecular modeling calculation demonstrating that JAT is mainly located within the hydrophobic pocket of the subdomain IIIA of BSA. Furthermore, The distance between donor (BSA) and acceptor (JAT) was estimated according to fluorescence resonance energy transfer.
authors: Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, Department of Chemistry, Hubei Normal University, Huangshi, 435002, People's Republic of China.
authors: Ran
authors: R
authors: Mi
authors: Pei-Qi
authors: PQ
authors: Li
authors: Yan-Jun
authors: YJ
authors: Hu
authors: Xiao-Yang
authors: XY
authors: Fan
authors: Hai-Ying
authors: HY
authors: Li
authors: Xue-Cheng
authors: XC
authors: Yu
authors: Yu
authors: Y
authors: Ouyang
mesh_terms: D001599:Berberine / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D001665:Binding Sites; D006860:Hydrogen Bonding; D057927:Hydrophobic and Hydrophilic Interactions; D008958:Models, Molecular; D008968:Molecular Conformation; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013057:Spectrum Analysis; D013816:Thermodynamics
chemical_list: C055785:jatrorrhizine; D001599:Berberine; D012710:Serum Albumin, Bovine
references: J Med Chem. 2008 Apr 10;51(7):2009-17
references: 18348514
references: J Mol Biol. 1993 Dec 5;234(3):779-815
references: 8254673
references: Colloids Surf B Biointerfaces. 2006 Aug 1;51(1):10-5
references: 16806852
references: J Agric Food Chem. 2012 Feb 22;60(7):1880-6
references: 22268806
references: Protein Pept Lett. 2004 Aug;11(4):307-15
references: 15327362
references: J Fluoresc. 2011 Mar;21(2):475-85
references: 20936333
references: Biochemistry. 1971 Aug 17;10(17):3254-63
references: 5119250
references: J Ethnopharmacol. 2011 May 17;135(2):561-8
references: 21466840
references: J Pharm Biomed Anal. 2009 Sep 8;50(2):107-16
references: 19473803
references: Biochemistry. 1981 May 26;20(11):3096-102
references: 7248271
references: Phys Chem Chem Phys. 2013 Jan 28;15(4):1275-87
references: 23232916
references: Cell Biochem Biophys. 2011 Nov;61(2):313-25
references: 21748244
references: Biomacromolecules. 2009 Mar 9;10(3):517-21
references: 19173654
references: Mol Biol Rep. 2012 May;39(5):5495-501
references: 22187343
references: J Phys Chem B. 2012 Aug 30;116(34):10195-204
references: 22834570
references: J Mol Biol. 2000 Nov 10;303(5):721-32
references: 11061971
references: Langmuir. 2009 Oct 6;25(19):11686-91
references: 19788221
references: Biochim Biophys Acta. 2008 Jul-Aug;1784(7-8):1020-7
references: 18456006
references: Biochem Biophys Res Commun. 2005 Jun 17;331(4):1284-94
references: 15883015
references: J Pharm Pharmacol. 2012 Mar;64(3):413-9
references: 22309273
references: Eur Biophys J. 2008 Oct;37(8):1303-8
references: 18463861
references: Biol Pharm Bull. 2009 Aug;32(8):1433-8
references: 19652386
references: J Phys Chem B. 2012 Mar 22;116(11):3504-11
references: 22364491
references: J Nat Prod. 2000 Dec;63(12):1638-40
references: 11141105
references: PLoS One. 2011;6(11):e26186
references: 22073150
references: J Pharm Biomed Anal. 2011 Jul 15;55(5):1223-6
references: 21458190
references: J Phys Chem B. 2010 Nov 25;114(46):14842-53
references: 21038894
references: Biomacromolecules. 2006 Apr;7(4):1350-6
references: 16602760
references: Neurosci Lett. 2011 Jul 12;498(3):227-31
references: 21605627
references: Eur J Pharmacol. 2011 Aug 1;663(1-3):74-9
references: 21596029
references: Pharmacol Rev. 1981 Mar;33(1):17-53
references: 7027277
references: Langmuir. 2012 Apr 10;28(14):5913-20
references: 22424242
references: Mol Pharmacol. 1976 Nov;12(6):1052-61
references: 1004490
references: Biomacromolecules. 2010 Feb 8;11(2):465-72
references: 20085247
references: Mol Biol Rep. 2012 Mar;39(3):2781-7
references: 21681428
references: Mol Pharmacol. 1975 Nov;11(6):824-32
references: 1207674
references: J Am Chem Soc. 2008 Sep 3;130(35):11677-88
references: 18681435
references: Biopharm Drug Dispos. 2012 Apr;33(3):135-45
references: 22344858
references: Molecules. 2012 Nov 22;17(12):13844-55
references: 23174900
references: Med Res Rev. 2011 Nov;31(6):821-62
references: 20077560
references: J Am Chem Soc. 2009 Aug 26;131(33):11644-5
references: 19645470
references: Biomacromolecules. 2010 Jan 11;11(1):106-12
references: 19899798
references: Chem Rev. 1948 Apr;42(2):285-410
references: 18914001
references: Phytochemistry. 2007 Jan;68(2):150-75
references: 17109902
references: Nature. 1992 Jul 16;358(6383):209-15
references: 1630489