title: [Plasma proteomic research on obesity subtype and non-obesity subtype of T2DM].
abstract: OBJECTIVE
  With the increasingly serious epidemic situation of diabetes, plasma proteomic method and OFFGEL electrophoresis have been applied for screening different proteins between obese and non-obese T2DM patients, which may be used to further explain the mechanism of T2DM.
  
  
  METHODS
  Twenty male T2DM volunteers (Obesity Subtype: 10; Non-obesity Subtype: 10) with the age of 18-44 years have been selected. The control group has been matched considering the factors of age, gender, etc. Albumin and IgG were removed from the plasma samples with highly specific immune-affinity method. Then the peptide-mixed samples were separated by pI with OFFGEL electrophoresis after solution digestion. Further separation and identification were performed by Nano HPLC-Chip-MS/MS system. Comparing the three groups, the differences were obtained and annotated on functions and its mechanism.
  
  
  RESULTS
  391, 415 and 371 proteins have been identified in the experimental groups and control group, respectively. The different proteins in groups and their annotations showed that adiponectin was down-regulated in obesity subtype of T2DM group, while STIM1 (stromal interaction molecule 1) was up-regulated. There were six protein kinases high expression in non-obesity DM patients, such as Serine-protein kinase ATM, Serine/threonine-protein kinase WNK1.
  
  
  CONCLUSIONS
  Adiponectin, STIM1 and protein kinases may act in different roles on the pathogenesis of obesity subtype and non-obesity subtype of T2DM.
authors: Shenzhen Center for Chronic Disease Control, Shenzhen 518020, China. junwangwh96@gmail.com
authors: Jun
authors: J
authors: Wang
authors: Wei
authors: W
authors: Yu
authors: Jian
authors: J
authors: Xu
authors: Liru
authors: L
authors: Feng
authors: Hui
authors: H
authors: Yang
authors: Xiaoli
authors: X
authors: Liu
mesh_terms: D052242:Adiponectin / Q000097:blood; D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000150:complications*; D004586:Electrophoresis; D005260:Female; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000097:blood; D009363:Neoplasm Proteins / Q000097:blood; D009765:Obesity / Q000097:blood* / Q000145:classification / Q000150:complications*; D011494:Protein Kinases / Q000097:blood; D020543:Proteome / Q000378:metabolism; D040901:Proteomics; D000071737:Stromal Interaction Molecule 1; D055815:Young Adult
chemical_list: D052242:Adiponectin; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D020543:Proteome; C103530:STIM1 protein, human; D000071737:Stromal Interaction Molecule 1; D011494:Protein Kinases