title: Associations between the human intestinal microbiota, Lactobacillus rhamnosus GG and serum lipids indicated by integrated analysis of high-throughput profiling data.
abstract: Accumulating evidence indicates that the intestinal microbiota regulates our physiology and metabolism. Bacteria marketed as probiotics confer health benefits that may arise from their ability to affect the microbiota. Here high-throughput screening of the intestinal microbiota was carried out and integrated with serum lipidomic profiling data to study the impact of probiotic intervention on the intestinal ecosystem, and to explore the associations between the intestinal bacteria and serum lipids. We performed a comprehensive intestinal microbiota analysis using a phylogenetic microarray before and after Lactobacillus rhamnosus GG intervention. While a specific increase in the L. rhamnosus-related bacteria was observed during the intervention, no other changes in the composition or stability of the microbiota were detected. After the intervention, lactobacilli returned to their initial levels. As previously reported, also the serum lipid profiles remained unaltered during the intervention. Based on a high-resolution microbiota analysis, intake of L. rhamnosus GG did not modify the composition of the intestinal ecosystem in healthy adults, indicating that probiotics confer their health effects by other mechanisms. The most prevailing association between the gut microbiota and lipid profiles was a strong positive correlation between uncultured phylotypes of Ruminococcus gnavus-group and polyunsaturated serum triglycerides of dietary origin. Moreover, a positive correlation was detected between serum cholesterol and Collinsella (Coriobacteriaceae). These associations identified with the spectrometric lipidome profiling were corroborated by enzymatically determined cholesterol and triglyceride levels. Actinomycetaceae correlated negatively with triglycerides of highly unsaturated fatty acids while a set of Proteobacteria showed negative correlation with ether phosphatidylcholines. Our results suggest that several members of the Firmicutes, Actinobacteria and Proteobacteria may be involved in the metabolism of dietary and endogenous lipids, and provide a scientific rationale for further human studies to explore the role of intestinal microbes in host lipid metabolism.
authors: Department of Veterinary Biosciences, University of Helsinki, Finland.|Laboratory of Microbiology, Wageningen University, Wageningen, Netherlands.
authors: Leo
authors: L
authors: Lahti
authors: Department of Veterinary Biosciences, University of Helsinki, Finland.|Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Finland.
authors: Anne
authors: A
authors: Salonen
authors: Valio R&D, Helsinki, Finland.
authors: Riina A
authors: RA
authors: Kekkonen
authors: Department of Veterinary Biosciences, University of Helsinki, Finland.
authors: Jarkko
authors: J
authors: Salojärvi
authors: Department of Veterinary Biosciences, University of Helsinki, Finland.
authors: Jonna
authors: J
authors: Jalanka-Tuovinen
authors: Department of Veterinary Biosciences, University of Helsinki, Finland.
authors: Airi
authors: A
authors: Palva
authors: Quantitative Biology and Bioinformatics, VTT Technical Research Centre of Finland, Espoo, Finland.
authors: Matej
authors: M
authors: Orešič
authors: Department of Veterinary Biosciences, University of Helsinki, Finland.|Laboratory of Microbiology, Wageningen University, Wageningen, Netherlands.|Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Finland.
authors: Willem M
authors: WM
authors: de Vos
keywords: Gastrointestinal tract; High-throughput profiling; Lactobacillus rhamnosus GG; Lipidomics; Microbiota; Physiology; Probiotics
references: Bioinformatics. 2009 Nov 1;25(21):2855-6
references: 19706745
references: Gastroenterology. 2011 Nov;141(5):1792-801
references: 21820992
references: J Nutr. 2007 Mar;137(3 Suppl 2):751S-5S
references: 17311972
references: Br J Nutr. 2011 Jul;106(1):6-14
references: 21385506
references: Clin Cancer Res. 2004 May 1;10(9):2922-7
references: 15131026
references: Eur J Pharmacol. 2011 Sep;668 Suppl 1:S50-8
references: 21816146
references: mBio. 2011 Mar 01;2(2):e00271-10
references: 21363910
references: Microbiology (Reading). 2009 Jan;155(Pt 1):285-294
references: 19118369
references: Nature. 2012 Sep 13;489(7415):242-9
references: 22972297
references: Bioinformatics. 2007 Feb 15;23(4):401-7
references: 17182697
references: PLoS One. 2009 Aug 20;4(8):e6669
references: 19693277
references: Int J Food Microbiol. 2011 Sep 1;149(1):50-7
references: 21296446
references: World J Gastroenterol. 2008 May 28;14(20):3188-94
references: 18506924
references: Br J Nutr. 2010 Jun;103(12):1778-83
references: 20100374
references: Appl Environ Microbiol. 2009 Jun;75(12):4175-84
references: 19411417
references: Gastroenterology. 2010 Dec;139(6):1961-1971.e1
references: 20600015
references: Microbiology (Reading). 2011 Feb;157(Pt 2):609-615
references: 21178166
references: World J Gastroenterol. 2008 Apr 7;14(13):2029-36
references: 18395902
references: Toxicol In Vitro. 2010 Jun;24(4):1266-72
references: 20123013
references: Gastroenterology. 2007 Jul;133(1):24-33
references: 17631127
references: J Lipid Res. 2010 May;51(5):1101-12
references: 20040631
references: Trends Microbiol. 2011 Jul;19(7):349-59
references: 21684749
references: Curr Opin Biotechnol. 2005 Apr;16(2):204-11
references: 15831388
references: Proc Natl Acad Sci U S A. 2011 Mar 15;108 Suppl 1:4523-30
references: 20837534
references: PLoS One. 2010 Jan 18;5(1):e8745
references: 20090909
references: Inflamm Bowel Dis. 2004 Nov;10(6):824-33
references: 15626901
references: Clin Microbiol Infect. 2012 Jul;18 Suppl 4:16-20
references: 22647042
references: Appl Environ Microbiol. 2012 Feb;78(4):933-40
references: 22101054
references: J Lipid Res. 2006 Feb;47(2):241-59
references: 16299351
references: Nature. 2010 Mar 4;464(7285):59-65
references: 20203603
references: Arterioscler Thromb Vasc Biol. 1997 Jun;17(6):1114-20
references: 9194762
references: Chem Phys Lipids. 2011 Sep;164(6):573-89
references: 21723266
references: Nutr Metab Cardiovasc Dis. 2011 Nov;21(11):844-50
references: 21930366
references: Gut. 2008 Nov;57(11):1605-15
references: 18941009
references: Proc Natl Acad Sci U S A. 2009 Oct 6;106(40):17193-8
references: 19805152
references: Br J Nutr. 2006 Feb;95(2):303-12
references: 16469146
references: Curr Opin Gastroenterol. 2008 Jan;24(1):51-8
references: 18043233
references: Syst Appl Microbiol. 2001 Jul;24(2):227-31
references: 11518325
references: BMC Microbiol. 2010 Jan 07;10:4
references: 20055983
references: Environ Microbiol. 2009 Jul;11(7):1736-51
references: 19508560
references: J Nutr Biochem. 2002 Jun;13(6):364-369
references: 12088802
references: J Appl Microbiol. 2009 Feb;106(2):506-14
references: 19200317
references: Gut. 2011 May;60(5):631-7
references: 21209126
references: Curr Opin Biotechnol. 1999 Oct;10(5):505-10
references: 10508641
references: Environ Microbiol. 2007 Sep;9(9):2125-36
references: 17686012
references: Crit Rev Biochem Mol Biol. 2002;37(4):259-81
references: 12236466
references: PLoS One. 2011;6(7):e23035
references: 21829582
references: Nature. 2011 Apr 7;472(7341):57-63
references: 21475195
references: Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9440-5
references: 12883005
references: Sci Transl Med. 2011 Oct 26;3(106):106ra106
references: 22030749
references: PLoS One. 2012;7(1):e29913
references: 22279554
references: Brief Bioinform. 2013 Jan;14(1):27-35
references: 22441573
references: Mol Syst Biol. 2007;3:112
references: 17515922
references: Stat Appl Genet Mol Biol. 2004;3:Article3
references: 16646809
references: Antimicrob Agents Chemother. 2011 Apr;55(4):1494-503
references: 21282433
references: Physiol Rev. 2010 Jul;90(3):859-904
references: 20664075
references: Diabetologia. 2009 Apr;52(4):684-90
references: 19214471
references: Trends Biotechnol. 2008 Dec;26(12):647-52
references: 18951641
references: Curr Med Chem. 2006;13(25):3005-21
references: 17073643
references: PLoS One. 2012;7(3):e33875
references: 22457798
references: J Microbiol Methods. 2010 May;81(2):127-34
references: 20171997