title: Oxidative stress mediates the disruption of airway epithelial tight junctions through a TRPM2-PLCγ1-PKCα signaling pathway.
abstract: Oxidative stress has been implicated as an important contributing factor in the pathogenesis of several pulmonary inflammatory diseases. Previous studies have indicated a relationship between oxidative stress and the attenuation of epithelial tight junctions (TJs). In Human Bronchial Epithelial-16 cells (16HBE), we demonstrated the degradation of zonula occludens-1 (ZO-1), and claudin-2 exhibited a great dependence on the activation of the transient receptor potential melastatin (TRPM) 2 channel, phospholipase Cγ1 (PLCγ1) and the protein kinase Cα (PKCα) signaling cascade.
authors: Department of Respiratory Medicine, the Second Affiliated Hospital, Chongqing University of Medical Science, Chongqing 400010, China. zxd999@263.net.
authors: Rui
authors: R
authors: Xu
authors: Qi
authors: Q
authors: Li
authors: Xiang-Dong
authors: XD
authors: Zhou
authors: Juliy M
authors: JM
authors: Perelman
authors: Victor P
authors: VP
authors: Kolosov
mesh_terms: D015153:Blotting, Western; D001980:Bronchi / Q000166:cytology*; D002460:Cell Line; D002463:Cell Membrane Permeability / Q000187:drug effects; D057167:Claudins / Q000378:metabolism; D017097:Electric Impedance; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D051966:Phospholipase C gamma / Q000378:metabolism*; D051571:Protein Kinase C-alpha / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D050053:TRPM Cation Channels / Q000378:metabolism*; D019108:Tight Junctions / Q000378:metabolism*; D062826:Zonula Occludens-1 Protein / Q000378:metabolism
chemical_list: D057167:Claudins; D034741:RNA, Small Interfering; D050053:TRPM Cation Channels; C121934:TRPM2 protein, human; D062826:Zonula Occludens-1 Protein; D006861:Hydrogen Peroxide; D051571:Protein Kinase C-alpha; D051966:Phospholipase C gamma
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