title: Improved xylose fermentation of Kluyveromyces marxianus at elevated temperature through construction of a xylose isomerase pathway.
abstract: To improve the xylose fermentation ability of Kluyveromyces marxianus, a xylose assimilation pathway through xylose isomerase was constructed. The genes encoding xylose reductase (KmXyl1) and xylitol dehydrogenase (KmXyl2) were disrupted in K. marxianus YHJ010 and the resultant strain was named YRL002. A codon-optimized xylose isomerase gene from Orpinomyces was transformed into K. marxianus YRL002 and expressed under GAPDH promoter. The transformant was adapted in the SD medium containing 1 % casamino acid with 2 % xylose as sole carbon source. After 32 times of trans-inoculation, a strain named YRL005, which can grow at a specific growth rate of 0.137/h with xylose as carbon source, was obtained. K. marxianus YRL005 could ferment 30.15 g/l of xylose and produce 11.52 g/l ethanol with a yield of 0.38 g/g, production rate of 0.069 g/l/h at 42 °C, and also could ferment 16.60 g/l xylose to produce 5.21 g/l ethanol with a yield of 0.31 g/g, and production rate of 0.054 g/l h at 45 °C. Co-fermentation with 2 % glucose could not improve the amount and yield of ethanol fermented from xylose obviously, but it could improve the production rate. Furthermore, K. marxianus YRL005 can ferment with the corn cob hydrolysate, which contained 20.04 g/l xylose to produce 8.25 g/l ethanol. It is a good platform to construct thermo-tolerant xylose fermentation yeast.
authors: School of Life Sciences, University of Science and Technology of China, Hefei, People's Republic of China.
authors: Rongliang
authors: R
authors: Wang
authors: Lulu
authors: L
authors: Li
authors: Biao
authors: B
authors: Zhang
authors: Xiaolian
authors: X
authors: Gao
authors: Dongmei
authors: D
authors: Wang
authors: Jiong
authors: J
authors: Hong
mesh_terms: D000449:Aldehyde Reductase / Q000235:genetics; D019747:Aldose-Ketose Isomerases / Q000235:genetics / Q000378:metabolism*; D050542:D-Xylulose Reductase / Q000235:genetics; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006358:Hot Temperature; D007716:Kluyveromyces / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism*
chemical_list: D000431:Ethanol; D014994:Xylose; D000449:Aldehyde Reductase; D050542:D-Xylulose Reductase; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase; D005947:Glucose
references: Appl Biochem Biotechnol. 1996 Spring;57-58:267-76
references: 8669900
references: Yeast. 1999 Aug;15(11):1021-30
references: 10455226
references: Anal Biochem. 1976 May 7;72:248-54
references: 942051
references: Bioresour Technol. 2001 Mar;77(1):57-63
references: 11211076
references: Appl Microbiol Biotechnol. 2004 Feb;63(5):578-83
references: 12925863
references: J Ind Microbiol Biotechnol. 2013 Apr;40(3-4):305-16
references: 23392758
references: Methods Enzymol. 2002;350:3-41
references: 12073320
references: Bioprocess Biosyst Eng. 2008 Jun;31(4):369-77
references: 17985160
references: J Biochem Biophys Methods. 2000 Nov 20;46(1-2):69-81
references: 11086195
references: Appl Environ Microbiol. 2009 Apr;75(8):2304-11
references: 19218403
references: Appl Environ Microbiol. 1982 Nov;44(5):1039-41
references: 16346128
references: Appl Microbiol Biotechnol. 2011 May;90(4):1573-86
references: 21476140
references: Appl Microbiol Biotechnol. 2009 Apr;82(6):1037-47
references: 19125247
references: Appl Microbiol Biotechnol. 1998 Sep;50(3):339-45
references: 9802219
references: Yeast. 2010 Jan;27(1):29-39
references: 19894210
references: Mol Biotechnol. 2013 Feb;53(2):159-69
references: 22351371
references: Mikrobiologiia. 2003 Jul-Aug;72(4):466-9
references: 14526534
references: Appl Microbiol Biotechnol. 2006 Feb;69(6):627-42
references: 16331454
references: Metab Eng. 2011 Sep;13(5):508-17
references: 21642010
references: Biosci Biotechnol Biochem. 2011;75(10):1994-2000
references: 21979076
references: Microbiology (Reading). 2007 Sep;153(Pt 9):3044-3054
references: 17768247
references: Appl Biochem Biotechnol. 2006 Jun;133(3):263-70
references: 16720906
references: Appl Environ Microbiol. 2008 Dec;74(24):7514-21
references: 18931291
references: Biotechniques. 2005 Oct;39(4):519-25
references: 16235564
references: Yeast. 2002 Oct;19(14):1203-20
references: 12271457
references: J Ind Microbiol Biotechnol. 2011 Dec;38(12):2001-10
references: 21643709
references: Appl Environ Microbiol. 1996 Dec;62(12):4648-51
references: 8953736
references: Appl Microbiol Biotechnol. 2011 Oct;92(1):77-84
references: 21655987
references: Bioresour Technol. 2010 Apr;101(7):2273-9
references: 19939673
references: Appl Environ Microbiol. 2005 Dec;71(12):8249-56
references: 16332810
references: Biotechnol Bioeng. 2006 Mar 5;93(4):665-73
references: 16372361
references: Appl Microbiol Biotechnol. 2010 Nov;88(5):1215-21
references: 20853104
references: Adv Biochem Eng Biotechnol. 2007;108:179-204
references: 17846724
references: Metab Eng. 2012 Nov;14(6):611-22
references: 22921355
references: Appl Microbiol Biotechnol. 2007 Apr;74(5):937-53
references: 17294186
references: Curr Opin Biotechnol. 2009 Jun;20(3):300-6
references: 19545992
references: FEMS Yeast Res. 2008 Feb;8(1):165-73
references: 17662053
references: Appl Microbiol Biotechnol. 2007 Jan;73(5):1039-46
references: 16977466
references: Appl Biochem Biotechnol. 2003 Spring;105 -108:141-53
references: 12721481
references: Appl Environ Microbiol. 2001 Dec;67(12):5668-74
references: 11722921
references: FEMS Yeast Res. 2005 Feb;5(4-5):399-409
references: 15691745
references: J Biotechnol. 2007 Jun 15;130(2):114-23
references: 17433483
references: J Ind Microbiol Biotechnol. 2003 May;30(5):279-91
references: 12698321
references: J Biol Chem. 1951 Oct;192(2):583-7
references: 14907652