title: Establishment of an in vitro model of the human placental barrier by placenta slice culture and ussing chamber.
abstract: Our purpose was to establish an in vitro model of the human placental barrier based on placenta slice culture and Ussing chamber. The villous morphology, beta-human chorionic gonadotropin (β-hCG), mRNA and efflux function of P-glycoprotein (P-gp), and the permeability of the fluorescent marker were confirmed. The results showed that syncytiotrophoblast cells with abundant endoplasmic reticulum and mitochondria were covered with a dense microvillus in the placenta slice. The β-hCG secretion levels in the Ussing chamber were 274.13 ± 13.52 mIU/mL at 5 h, significantly higher than that in the incubator 95.2 ± 13.14 mIU/mL, and β-hCG continued to secrete for 48 h. P-gp mRNA was expressed in the placenta slice. The Rho123 apparent permeability coefficient (Papp) value from maternal side to the fetal side was 26.34 ± 1.87 nm/s, but it was significantly increased, to 289.55 ± 6.02 nm/s after adding verapamil. The Rho123 efflux value was >2. The fluorescein Papp value was (3.42 ± 0.24) × 10(-3) nm/s. In contrast, the fluorescein isothiocyanate-dextran (FD70) Papp value was (3.93 ± 0.08) × 10(-5) nm/s. This indicates that the placenta slice in the Ussing chamber had the activity of a placenta, and can act as a valuable in vitro model of placental barrier.
authors: Department of Obstetrics and Gynecology, Second Hospital Affiliated to Tianjin University of Traditional Chinese Medicine, Tianjin, PR China. Songdr58@126.com
authors: Dianrong
authors: D
authors: Song
authors: Jie
authors: J
authors: Guo
authors: Fang
authors: F
authors: Han
authors: Wei
authors: W
authors: Zhang
authors: Yanan
authors: Y
authors: Wang
authors: Yuhua
authors: Y
authors: Wang
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics; D006063:Chorionic Gonadotropin / Q000378:metabolism; D002824:Chorionic Villi / Q000648:ultrastructure; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D010539:Permeability; D010920:Placenta / Q000166:cytology* / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D046509:Tissue Culture Techniques / Q000379:methods*
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D006063:Chorionic Gonadotropin; D012333:RNA, Messenger