title: Nuclear translocation and accumulation of glyceraldehyde-3-phosphate dehydrogenase involved in diclazuril-induced apoptosis in Eimeria tenella (E. tenella).
abstract: In mammalian cells, GAPDH (glyceraldehyde-3-phosphate dehydrogenase) has recently been shown to be implicated in numerous apoptotic paradigms, especially in neuronal apoptosis, and has been demonstrated to play a vital role in some neurodegenerative disorders. However, this phenomenon has not been reported in protists. In the present study, we report for the first time that such a mechanism is involved in diclazuril-induced apoptosis in Eimeria tenella (E. tenella). We found that upon treatment of parasites with diclazuril, the expression levels of GAPDH transcript and protein were significantly increased in second-generation merozoites. Then, we examined the subcellular localization of GAPDH by fluorescence microscopy and Western blot analysis. The results show that a considerable amount of GAPDH protein appeared in the nucleus within diclazuril-treated second-generation merozoites; in contrast, the control group had very low levels of GAPDH in the nucleus. The glycolytic activity of GAPDH was kinetically analyzed in different subcellular fractions. A substantial decrease (48.5%) in glycolytic activity of GAPDH in the nucleus was displayed. Moreover, the activities of caspases-3, -9, and -8 were measured in cell extracts using specific caspase substrates. The data show significant increases in caspase-3 and caspase-9 activities in the diclazuril-treated group.
authors: Key Laboratory of Veterinary Drug Safety Evaluation and Residues Research, Chinese Academy of Agricultural Sciences, Shanghai Veterinary Research Institute, CAAS, Shanghai, 200241, PR China. litao@shvri.ac.cn.
authors: Congcong
authors: C
authors: Wang
authors: Chunzhou
authors: C
authors: Han
authors: Tao
authors: T
authors: Li
authors: Dehao
authors: D
authors: Yang
authors: Xiaojiong
authors: X
authors: Shen
authors: Yinxin
authors: Y
authors: Fan
authors: Yang
authors: Y
authors: Xu
authors: Wenli
authors: W
authors: Zheng
authors: Chenzhong
authors: C
authors: Fei
authors: Lifang
authors: L
authors: Zhang
authors: Feiqun
authors: F
authors: Xue
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D020169:Caspases / Q000378:metabolism; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D002645:Chickens; D003049:Coccidiostats / Q000494:pharmacology*; D016786:Eimeria tenella / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000378:metabolism*; D008297:Male; D052940:Merozoites / Q000187:drug effects / Q000254:growth & development / Q000502:physiology; D009570:Nitriles / Q000494:pharmacology*; D011897:Random Allocation; D014227:Triazines / Q000494:pharmacology*
chemical_list: D003049:Coccidiostats; D009570:Nitriles; D014227:Triazines; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D020169:Caspases; C057884:diclazuril
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