title: Study on a biomimetic enzyme-linked immunosorbent assay method for rapid determination of trace acrylamide in French fries and cracker samples.
abstract: BACKGROUND
  Acrylamide has attracted worldwide concern due to its neurotoxicity, genotoxicity, reproductive-development toxicity. It is necessary to develop an accurate and reliable analytical method to prevent the harm on the human health.
  
  
  RESULTS
  In this study, a sensitive and fast analytical method of direct competitive biomimetic enzyme-linked immunosorbent assay (BELISA) was developed using a hydrophilic imprinted membrane as biomimetic antibody. This novel imprinted membrane was directly synthesised on the well surface of MaxiSorp polystyrene 96-well plates in an aqueous environment, and it exhibited high binding ability and specificity toward acrylamide. Under the optimal conditions, the established BELISA method had a good sensitivity (IC50, 8.0 ± 0.4 mg L(-1)) and a low limit of detection (IC15, 85.0 ± 4.2 µg L(-1)). The blank potato samples spiked with acrylamide at three levels of 100, 250 and 500 µg L(-1) were extracted and determinate by the proposed method, and good recoveries ranging from 90.0% to 110.5% were obtained. This presented method was applied to the quantitative detection of the acrylamide in French fries and cracker samples. Also, the results were correlated well with that obtained by the gas chromatography method.
  
  
  CONCLUSIONS
  With good properties of high sensitivity, simple pre-treatment and low cost, this BELISA could be a promising screening method in food sample analysis.
authors: College of Food Science and Engineering, Shandong Agricultural University, Tai'an, 271018, People's Republic of China.
authors: Qing
authors: Q
authors: Sun
authors: Longhua
authors: L
authors: Xu
authors: Yue
authors: Y
authors: Ma
authors: Xuguang
authors: X
authors: Qiao
authors: Zhixiang
authors: Z
authors: Xu
mesh_terms: D020106:Acrylamide / Q000032:analysis*; D032701:Biomimetics; D001939:Bread / Q000032:analysis; D002849:Chromatography, Gas; D004797:Enzyme-Linked Immunosorbent Assay / Q000295:instrumentation / Q000379:methods*; D005504:Food Analysis / Q000379:methods; D005506:Food Contamination / Q000032:analysis; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D012680:Sensitivity and Specificity; D011198:Solanum tuberosum / Q000737:chemistry*
chemical_list: D020106:Acrylamide
keywords: acrylamide; biomimetic antibody; enzyme-linked immunosorbent assay; hydrophilic imprinted membrane