title: Identification of bifidobacteria isolated from Asian elephant (Elephas maximus).
abstract: Bifidobacteria are considered as one of the key genera in intestinal tracts of animals, and their species composition vary depending on the host. The aim of this study was to identify faecal bifidobacteria from Asian elephants (Elephas maximus), housed in Zoological gardens (Ostrava, Czech Republic). Using culturing, bifidobacteria were found in counts 7.60+/-0.56 log CFU/g. Twenty-six pure strains were isolated from faeces of Asian elephant. The isolates were clustered into two groups according to fingerprinting profiles and fermentation characteristic. Bacteria were identified by a combination of MALDI-TOF MS, PCR methods and sequencing as B. boum (12 isolates) and B. adolescentis (14 isolates). Elephant strains showed different fingerprinting profiles than type and collection strains. Since these two species are frequently isolated from gastrointestinal tract of herbivores, they seem to be typical of animals fed plant diets.
authors: Department of Microbiology, Nutrition and Dietetics, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamycka 129, Prague 6-Suchdol 1652 1, Czech Republic.
authors: Vera
authors: V
authors: Bunesova
authors: Eva
authors: E
authors: Vlkova
authors: Vojtech
authors: V
authors: Rada
authors: Jiri
authors: J
authors: Killer
authors: Vladimir
authors: V
authors: Kmet
mesh_terms: D000818:Animals; D001644:Bifidobacterium / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D016172:DNA Fingerprinting; D004606:Elephants / Q000382:microbiology*; D005243:Feces / Q000382:microbiology*; D005285:Fermentation; D058889:Molecular Typing; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S
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