title: The stimulated innate resistance event in Bordetella pertussis infection is dependent on reactive oxygen species production.
abstract: The exacerbated induction of innate immune responses in airways can abrogate diverse lung infections by a phenomenon known as stimulated innate resistance (StIR). We recently demonstrated that the enhancement of innate response activation can efficiently impair Bordetella pertussis colonization in a Toll-like receptor 4 (TLR4)-dependent manner. The aim of this work was to further characterize the effect of lipopolysaccharide (LPS) on StIR and to identify the mechanisms that mediate this process. Our results showed that bacterial infection was completely abrogated in treated mice when the LPS of B. pertussis (1 μg) was added before (48 h or 24 h), after (24 h), or simultaneously with the B. pertussis challenge (10(7) CFU). Moreover, we detected that LPS completely cleared bacterial infection as soon as 2 h posttreatment. This timing suggests that the observed StIR phenomenon should be mediated by fast-acting antimicrobial mechanisms. Although neutrophil recruitment was already evident at this time point, depletion assays using an anti-GR1 antibody showed that B. pertussis clearance was achieved even in the absence of neutrophils. To evaluate the possible role of free radicals in StIR, we performed animal assays using the antioxidant N-acetyl cysteine (NAC), which is known to inactivate oxidant species. NAC administration blocked the B. pertussis clearance induced by LPS. Nitrite concentrations were also increased in the LPS-treated mice; however, the inhibition of nitric oxide synthetases did not suppress the LPS-induced bacterial clearance. Taken together, our results show that reactive oxygen species (ROS) play an essential role in the TLR4-dependent innate clearance of B. pertussis.
authors: Laboratorio VacSal, Instituto de Biotecnología y Biología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CONICET, La Plata, Buenos Aires, Argentina.
authors: E
authors: E
authors: Zurita
authors: G
authors: G
authors: Moreno
authors: A
authors: A
authors: Errea
authors: M
authors: M
authors: Ormazabal
authors: M
authors: M
authors: Rumbo
authors: D
authors: D
authors: Hozbor
mesh_terms: D000111:Acetylcysteine / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D058491:Bacterial Load; D001885:Bordetella Infections / Q000276:immunology* / Q000382:microbiology; D001886:Bordetella pertussis / Q000187:drug effects / Q000276:immunology / Q000472:pathogenicity*; D006146:Guanidines / Q000494:pharmacology; D007113:Immunity, Innate; D008070:Lipopolysaccharides / Q000276:immunology / Q000494:pharmacology; D008168:Lung / Q000276:immunology / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000276:immunology*; D013997:Time Factors; D051197:Toll-Like Receptor 4 / Q000276:immunology
chemical_list: D006146:Guanidines; D008070:Lipopolysaccharides; D009573:Nitrites; D017382:Reactive Oxygen Species; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; D019001:Nitric Oxide Synthase; C004479:pimagedine; D000111:Acetylcysteine
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