title: Aloe vera gel protects liver from oxidative stress-induced damage in experimental rat model.
abstract: Aloe vera is a semi-tropical plant of Liliaceae family which has a wide range of applications in traditional medicine. In the present study, we sought to investigate the heptaoprotective potential of Aloe vera gel as a diet supplement. To achieve this goal, we have designed in vitro and in vivo experimental models of chemical-induced liver damage using male Sprague-Dawley rat. In the in vitro model, its effect was evaluated on Fenton's reaction-induced liver lipid peroxidation. Co-incubation with gel significantly reduced the generation of liver lipid peroxide (LPO). Next, to see the similar effect in vivo, gel was orally administered to rats once daily for 21 successive days. Following 1 hour of the last administration of gel, rats were treated with intra-peritoneal injection of CCl4. Dietary gel showed significant hepatoprotection against CCl4-induced damage as evident by restoration of liver LPO, serum transaminases, alkaline phosphatase, and total bilirubin towards near normal. The beneficial effects were pronounced with the doses used (400 and 800 mg/kg body weight). Besides, we did not observe any significant drop in serum albumin, globulin as well as total protein levels of gel-administered rats. Histopathology of the liver tissue further supported the biochemical findings confirming the hepatoprotective potential of dietary gel.
authors: Department of Biochemistry and Molecular Biology, Jahangirnagar University, Dhaka, Savar, Bangladesh. taslima_bmb@juniv.edu
authors: Taslima
authors: T
authors: Nahar
authors: Borhan
authors: B
authors: Uddin
authors: Shahdat
authors: S
authors: Hossain
authors: Abdul Mannan
authors: AM
authors: Sikder
authors: Sohel
authors: S
authors: Ahmed
mesh_terms: D000469:Alkaline Phosphatase / Q000097:blood; D000504:Aloe; D000818:Animals; D001663:Bilirubin / Q000378:metabolism; D001798:Blood Proteins / Q000378:metabolism; D002251:Carbon Tetrachloride; D056486:Chemical and Drug Induced Liver Injury / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D019587:Dietary Supplements; D005782:Gels; D005916:Globulins / Q000378:metabolism; D006861:Hydrogen Peroxide; D007501:Iron; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D008517:Phytotherapy; D028321:Plant Preparations / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D012709:Serum Albumin / Q000378:metabolism; D000637:Transaminases / Q000097:blood
chemical_list: D001798:Blood Proteins; C045076:Fenton's reagent; D005782:Gels; D005916:Globulins; D028321:Plant Preparations; D012709:Serum Albumin; D006861:Hydrogen Peroxide; D002251:Carbon Tetrachloride; D007501:Iron; D000637:Transaminases; D000469:Alkaline Phosphatase; D001663:Bilirubin