title: Ductal plate in hepatoblasts in human fetal livers: I. ductal plate-like structures with cytokeratins 7 and 19 are occasionally seen within human fetal hepatoblasts.
abstract: The author has studied the organogenesis of human intrahepatic bile duct in fetal livers. The developmental studies of the liver have focused mainly on the development of intrahepatic bile ducts including the ductal plate (DP). The DP is a single or double layered structures composed of small cuboidal cells and located in the interface between the hepatoblasts and portal mesenchyme. Herein, the author discovered the DP within the parenchymal hepatocytes. The DP-like structures within the hepatoblasts were found in 17 human fetal livers out of the 32 human fetal livers. The gestational ages of the 17 fetal livers were as follows: 7, 8, 9, 10 (n=2), 11, 12, 13, 14 (n=2), 15, 16, 17, 18, 19, 21, and 23 weeks. The presence of intraparenchymal DP-like structures were mainly found in the fetus of early gestational ages. Morphologically, the DP-like structures within the hepatoblasts were composed of small cuboidal epithelial cells with normal chromatin patterns. The cytoplasm was scant and relatively basophilic. The nuclei were small and round, and had no nucleoli. These cells formed the DP-like structures. The DP-like structures frequently formed cords, tubules, and duplicating patterns. These DP-like structures were scattered and the remaining hepatoblasts are normal hepatoblasts. The density of these DP-structures was low (one or two per 5 low power fields), but varied from case to case and area to area in the same case. The overall appearances were very similar to the true DP. Comparative observations of HE and CK immunostaining were performed. The DP-like structures within the hepatoblasts were positive for biliary-type CK7 and CK19. They were also positive for CK8 and CK18 that are expressed in both hepatocyte and biliary lineages. The true DP was positive for biliary-type CK7 and CK19. They were also positive for CK8 and CK18. Thus, the intraparenchymal DP-like structures were the same as the true DP located in the interface. Thus, the author discovered the intraparenchymal DP in the human fetal livers. This discovery should be confirmed by other researchers. If it is true, many studies of the functions of these intraparenchymal DPs are need.
authors: Department of Pathology, Shizuoka City Shimizu Hospital, Shizuoka, Japan. piyo0111jp@yahoo.co.jp
authors: Tadashi
authors: T
authors: Terada
mesh_terms: D001653:Bile Ducts, Intrahepatic / Q000196:embryology* / Q000378:metabolism; D005333:Fetus; D022781:Hepatocytes / Q000378:metabolism / Q000648:ultrastructure*; D006801:Humans; D007150:Immunohistochemistry; D053552:Keratin-7 / Q000032:analysis / Q000096:biosynthesis*; D053534:Keratin-9 / Q000032:analysis / Q000096:biosynthesis*
chemical_list: D053552:Keratin-7; D053534:Keratin-9
keywords: Ductal plate; fetal liver; hepatoblasts; immunohistochemistry; liver development
references: Hepatology. 1994 Aug;20(2):388-97
references: 8045500
references: J Hepatol. 1986;2(2):141-56
references: 3958471
references: Hepatology. 2012 Nov;56(5):2004-5
references: 22473877
references: Tohoku J Exp Med. 1997 Jan;181(1):19-32
references: 9149336
references: Arch Pathol Lab Med. 1990 Jun;114(6):597-600
references: 2189374
references: Hepatology. 1990 Nov;12(5):1229-33
references: 1699863
references: Am J Pathol. 1995 Jan;146(1):67-74
references: 7531950
references: Hepatology. 1993 Oct;18(4):803-8
references: 8406353
references: J Hepatol. 1989 Mar;8(2):139-49
references: 2541196
references: Am J Pathol. 1995 Sep;147(3):574-9
references: 7677170
references: Hepatol Res. 2011 Jan;41(1):93-8
references: 21199202
references: Hepatology. 1992 Oct;16(4):1069-83
references: 1398487
references: Am J Gastroenterol. 1987 Dec;82(12):1301-5
references: 3318404
references: Acta Pathol Microbiol Scand Suppl. 1977 Jan;(257):1-87
references: 319621
references: Appl Immunohistochem Mol Morphol. 2011 Oct;19(5):450-3
references: 21403518
references: Hepatology. 1996 Mar;23(3):476-81
references: 8617427
references: Pathol Oncol Res. 2011 Mar;17(1):159-65
references: 20512666
references: Histopathology. 1994 Aug;25(2):143-50
references: 7527010
references: Hepatology. 1993 Sep;18(3):529-36
references: 8359795
references: Virchows Arch A Pathol Anat Histopathol. 1988;412(4):339-45
references: 3125672
references: Liver. 2000 Dec;20(6):475-81
references: 11169062
references: Liver. 1982 Dec;2(4):346-54
references: 7167037
references: Arch Pathol Lab Med. 1988 Nov;112(11):1113-6
references: 3178424
references: Liver. 2000 Dec;20(6):450-7
references: 11169059
references: Hepatology. 1990 Jul;12(1):92-7
references: 2165033
references: J Hepatol. 1998 Feb;28(2):263-9
references: 9514539
references: Gastroenterology. 1995 Apr;108(4):1236-45
references: 7535276
references: Virchows Arch A Pathol Anat Histopathol. 1988;413(2):167-76
references: 3133876
references: Lab Invest. 1993 Mar;68(3):261-9
references: 7680729
references: J Hepatol. 1985;1(5):545-59
references: 4056355
references: Hum Pathol. 1992 May;23(5):483-90
references: 1568745
references: Histol Histopathol. 2003 Jan;18(1):129-34
references: 12507292
references: Arch Pathol Lab Med. 1989 Oct;113(10):1135-8
references: 2478106
references: Hepatology. 1995 Jul;22(1):75-81
references: 7601436
references: Am J Surg Pathol. 2012 Nov;36(11):1629-35
references: 23073321
references: Pathol Int. 2002 Nov;52(11):740-6
references: 12685552
references: Pathol Int. 2010 May;60(5):407-12
references: 20518892
references: Virchows Arch. 1994;424(6):669-75
references: 8055162
references: Am J Pathol. 1995 Nov;147(5):1207-13
references: 7485384
references: Tohoku J Exp Med. 2005 Jul;206(3):271-5
references: 15942157
references: Virchows Arch. 2011 Mar;458(3):251-9
references: 21287200
references: Hepatology. 1988 Nov-Dec;8(6):1586-95
references: 2461337
references: J Histochem Cytochem. 1992 Nov;40(11):1627-35
references: 1431051
references: J Hepatol. 1989 Sep;9(2):141-8
references: 2809154
references: Liver. 1998 Oct;18(5):313-9
references: 9831359
references: Anat Rec (Hoboken). 2008 Jun;291(6):628-35
references: 18484608
references: Liver. 1987 Feb;7(1):1-8
references: 3553822
references: Gastroenterology. 2011 Oct;141(4):1432-8, 1438.e1-4
references: 21708104
references: Gastroenterology. 1990 May;98(5 Pt 1):1333-7
references: 2182371
references: Hepatol Res. 2012 Jul;42(7):721-6
references: 22686860