title: ¹H, ¹³C and ¹⁵N resonance assignments for the fibrillin-1 EGF2-EGF3-hybrid1-cbEGF1 four-domain fragment.
abstract: Fibrillins are large extracellular glycoproteins that form the principal component of microfibrils. These perform a vital structural function in the extracellular matrix of many tissues. Fibrillins have also been implicated in mediating a number of protein-protein interactions, some of which may be significant in regulating growth factors such as transforming growth factor β. Here we present the backbone and side-chain (1)H, (13)C and (15)N assignments for a 19 kDa protein fragment derived from the N-terminus of human fibrillin-1, encompassing four domains in total. These domains include the second and third epidermal growth factor-like (EGF) domains, the first hybrid domain (hyb1), and the first calcium-binding EGF domain of fibrillin-1. This region of fibrillin-1 is of particular interest as the hyb1 domain has been suggested to play a role in microfibril assembly, as well as several other protein-protein interactions.
authors: Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK.
authors: Ian B
authors: IB
authors: Robertson
authors: Isabelle
authors: I
authors: Osuch
authors: David A
authors: DA
authors: Yadin
authors: Penny A
authors: PA
authors: Handford
authors: Sacha A
authors: SA
authors: Jensen
authors: Christina
authors: C
authors: Redfield
mesh_terms: D000595:Amino Acid Sequence; D002118:Calcium / Q000378:metabolism*; D002247:Carbon Isotopes; D004815:Epidermal Growth Factor / Q000737:chemistry*; D000071838:Fibrillin-1; D000071837:Fibrillins; D006801:Humans; D006859:Hydrogen; D008840:Microfilament Proteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D017434:Protein Structure, Tertiary; D054392:Adipokines
chemical_list: D002118:Calcium; D002247:Carbon Isotopes; D004815:Epidermal Growth Factor; D000071838:Fibrillin-1; D000071837:Fibrillins; D006859:Hydrogen; D008840:Microfilament Proteins; D009587:Nitrogen Isotopes; C000605722:FBN1 protein, human; D054392:Adipokines
references: Proteins. 2005 Jun 1;59(4):687-96
references: 15815974
references: Biochem J. 2011 Jan 15;433(2):263-76
references: 21175431
references: Biochim Biophys Acta. 2000 Dec 20;1498(2-3):84-90
references: 11108952
references: Science. 2011 Apr 15;332(6027):361-5
references: 21493863
references: Structure. 2004 Apr;12(4):717-29
references: 15062093
references: Structure. 2012 Feb 8;20(2):215-25
references: 22325771
references: Biomol NMR Assign. 2014 Apr;8(1):75-80
references: 23264024
references: J Struct Biol. 2006 Aug;155(2):285-93
references: 16697222
references: J Cell Sci. 2010 Sep 1;123(Pt 17):3006-18
references: 20699357
references: Cell. 1996 May 17;85(4):597-605
references: 8653794
references: J Biol Chem. 2009 Jun 19;284(25):16872-16881
references: 19349279
references: J Biol Chem. 2000 Jan 21;275(3):2205-10
references: 10636927
references: J Mol Biol. 1996 Jan 12;255(1):22-7
references: 8568869
references: J Biomol NMR. 1995 Nov;6(3):277-93
references: 8520220
references: J Biol Chem. 2003 Apr 4;278(14):12199-206
references: 12511552
references: Nat Genet. 2003 Mar;33(3):407-11
references: 12598898
references: J Clin Invest. 2004 Dec;114(11):1586-92
references: 15546004
references: Structure. 2009 May 13;17(5):759-68
references: 19446531
references: Nat Med. 2007 Feb;13(2):204-10
references: 17237794
references: Science. 2006 Apr 7;312(5770):117-21
references: 16601194
references: J Biol Chem. 2003 Jan 24;278(4):2750-7
references: 12429738