title: Validation of an LC-MS bioanalytical method for quantification of phytate levels in rat, dog and human plasma.
abstract: Myo-inositol hexakisphosphate (phytate, IP6) is a naturally occuring compound whose determination in biological matrices is chanllenging. Several benefitial properties have been attributed to IP6 in parallel with the development of suitable analytical methodologies for its analytical determination in urine and some tissues. However, there is a lack of appropriate tools for its determination in plasma samples. In this paper, a direct, sensitive and selective bioanalytical method for the determination of IP6 based on LC-MS is presented. It is the first method published to quantify IP6 in plasma matrices directly through its molecular weight, being consequently a highly specific methodology. The method has been validated in rat, dog and human plasma, according to the acceptance criteria laid down in the FDA guidance Bioanalytical Method Validation. Accuracy and precision were not greater than 15% at medium and high concentrations and not greater than 20% at the LLOQ concentration. The mean absolute recovery obtained ranged from 78.74 to 102.44%, 62.10 to 87.21% and 61.61 to 86.99% for rat, dog and human plasma respectively. The LLOQ was 500ngmL(-1) due to the presence of endogenous IP6 in blank plasma samples and the limit of detection was within the range 30-80ngmL(-1).
authors: Sanifit Laboratoris S.L. Research and Development Department, 07121 Palma de Mallorca, Spain.
authors: Fernando
authors: F
authors: Tur
authors: Eva
authors: E
authors: Tur
authors: Irene
authors: I
authors: Lentheric
authors: Paula
authors: P
authors: Mendoza
authors: Maximo
authors: M
authors: Encabo
authors: Bernat
authors: B
authors: Isern
authors: Felix
authors: F
authors: Grases
authors: Ciriaco
authors: C
authors: Maraschiello
authors: Joan
authors: J
authors: Perelló
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004285:Dogs; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D013058:Mass Spectrometry / Q000379:methods*; D010833:Phytic Acid / Q000097:blood*; D051381:Rats
chemical_list: D010833:Phytic Acid