title: Species of Candida as a component of the nasal microbiota of healthy horses.
abstract: Respiratory infections are a common problem among equines and occur with variable rates of morbidity and mortality. Although some fungal species are considered primary agents of respiratory tract infections in several mammals, their relevance in respiratory diseases of equines is frequently neglected. In the present study, we performed an active search for Candida spp. in the nasal cavity of horses. The presence of Candida spp. was investigated through the use of nasal swabs that were streaked on culture media. These yeasts were identified through physiological testing and their in vitro antifungal susceptibility were also characterized. The analysis of the material from the nasal cavity of 97 randomly chosen horses resulted in the isolation of Candida spp. from 35 animals (36.08%), out of which 18 (32.14%) were C. famata, 14 (25%) C. parapsilosis, 12 (21.42%) Meyerozyma guilliermondii (C. guilliermondii), 11 (19.64%) C. tropicalis and 1 (1.78%) Wickerhamomyces anomalus (C. pelliculosa). The minimum inhibitory concentration (MIC) values ranged from 0.03125-1 μg/ml for amphotericin B; and from 0.03125-> 16 μg/ml and 0.125 to > 64 μg/ml for itraconazole and fluconazole, respectively. Resistance to fluconazole and itraconazole was observed among C. tropicalis (n = 3) and C. guilliermondii (n = 1). The data show a predominance of non-C. albicans Candida species in the nasal microbiota of healthy equines, including antifungal resistant isolates, reiterating the importance of monitoring fungal pathogens in these animals.
authors: * Department of Pathology and Legal Medicine, School of Medicine, Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Federal University of Ceará , Fortaleza-CE , Brazil.
authors: Rossana De Aguiar
authors: Rde A
authors: Cordeiro
authors: Paula Vago
authors: PV
authors: Bittencourt
authors: Raimunda Sâmia Nogueira
authors: RS
authors: Brilhante
authors: Carlos Eduardo Cordeiro
authors: CE
authors: Teixeira
authors: Débora De Souza Collares Maia
authors: Dde S
authors: Castelo-Branco
authors: Sabrina Tainah Da Cruz
authors: ST
authors: Silva
authors: Lucas Pereira
authors: LP
authors: De Alencar
authors: Elizabeth Ribeiro Yokobatake
authors: ER
authors: Souza
authors: Tereza de Jesus Pinheiro Gomes
authors: Tde J
authors: Bandeira
authors: André Jalles
authors: AJ
authors: Monteiro
authors: José Júlio Costa
authors: JJ
authors: Sidrim
authors: Marcos Fábio Gadelha
authors: MF
authors: Rocha
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology; D002175:Candida / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification*; D005260:Female; D006736:Horses / Q000382:microbiology*; D008297:Male; D008828:Microbiological Techniques / Q000379:methods; D064307:Microbiota; D009172:Mycology / Q000379:methods; D009297:Nasal Mucosa / Q000382:microbiology*
chemical_list: D000935:Antifungal Agents