title: Problem-solving test: digestion of a plasmid with restriction endonucleases.
abstract: Terms to be familiar with before you start to solve the test: plasmid, restriction endonuclease, agarose gel electrophoresis, ethidium bromide staining, autoradiography, Coomassie staining, Southern blotting, linear and circular DNA, superhelical DNA, exonuclease, modification methylase, palindrome, sticky and blunt ends, nicked circular DNA.
authors: Department of Medical Biology, Medical School, University of Pécs, H-7624 Pécs, Hungary. jozsef.szeberenyi@aok.pte.hu
authors: József
authors: J
authors: Szeberényi
mesh_terms: D015139:Blotting, Southern / Q000379:methods*; D004262:DNA Restriction Enzymes / Q000235:genetics / Q000378:metabolism*; D004270:DNA, Circular; D004278:DNA, Superhelical; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D004996:Ethidium; D006801:Humans; D008967:Molecular Biology / Q000193:education*; D010957:Plasmids; D018794:Problem-Based Learning / Q000379:methods*; D013334:Students
chemical_list: D004270:DNA, Circular; D004278:DNA, Superhelical; D004262:DNA Restriction Enzymes; D004996:Ethidium