title: Fluorescence molecular tomography of brain tumors in mice.
abstract: Fluorescence molecular tomography of tissues is a method that three-dimensionally resolves fluorescence biodistribution in vivo, with applications in small-animal research and pre-clinical diagnostics. There are many alternative imaging geometries in optical tomographic experimental systems, but in general, all imaging setups consist of four subsystems: illumination, animal mount, imaging, and automation and data acquisition (i.e., electronics and computer). Here we refer to charge-coupled device (CCD)-based systems that work in trans-illumination (i.e., illumination and detection occur on opposite sides of the subject), while a mouse or other small animal is rotated through 360° to allow photon acquisition from multiple projections. We present a procedure to tomographically reconstruct the biodistribution of fluorescence in small animals. The imaging system and equipment are described, the step-by-step image acquisition and preliminary image-processing methods are presented, and the tomographic reconstruction procedure is outlined. Finally, the method is showcased by imaging the fluorescence activity of a brain tumor of a glioblastoma mouse model.
authors: Nikolaos C
authors: NC
authors: Deliolanis
authors: Vasilis
authors: V
authors: Ntziachristos
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000473:pathology*; D005453:Fluorescence; D021621:Imaging, Three-Dimensional / Q000379:methods; D051379:Mice; D010336:Pathology / Q000379:methods*; D014054:Tomography / Q000379:methods*