title: Enterococcal Rgg-like regulator ElrR activates expression of the elrA operon.
abstract: The Enterococcus faecalis leucine-rich protein ElrA promotes virulence by stimulating bacterial persistence in macrophages and production of the interleukin-6 (IL-6) cytokine. The ElrA protein is encoded within an operon that is poorly expressed under laboratory conditions but induced in vivo. In this study, we identify ef2687 (renamed elrR), which encodes a member of the Rgg (regulator gene for glucosyltransferase) family of putative regulatory proteins. Using quantitative reverse transcription-PCR, translational lacZ fusions, and electrophoretic mobility shift assays, we demonstrate that ElrR positively regulates expression of elrA. These results correlate with the attenuated virulence of the ΔelrR strain in a mouse peritonitis model. Virulence of simple and double elrR and elrA deletion mutants also suggests a remaining ElrR-independent expression of elrA in vivo and additional virulence-related genes controlled by ElrR.
authors: INRA, UMR1319 Micalis, Jouy-en-Josas, France.
authors: Romain
authors: R
authors: Dumoulin
authors: Naima
authors: N
authors: Cortes-Perez
authors: Stephane
authors: S
authors: Gaubert
authors: Philippe
authors: P
authors: Duhutrel
authors: Sophie
authors: S
authors: Brinster
authors: Riccardo
authors: R
authors: Torelli
authors: Maurizio
authors: M
authors: Sanguinetti
authors: Brunella
authors: B
authors: Posteraro
authors: Francis
authors: F
authors: Repoila
authors: Pascale
authors: P
authors: Serror
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D013293:Enterococcus faecalis / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity*; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics / Q000502:physiology*; D051379:Mice; D009876:Operon / Q000235:genetics / Q000502:physiology*; D014774:Virulence / Q000235:genetics / Q000502:physiology
chemical_list: D001426:Bacterial Proteins
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