title: A real-time PCR for the detection of infectious myonecrosis virus in penaeid shrimp.
abstract: Infectious myonecrosis virus (IMNV) is a recently observed shrimp virus, which threats the cultured Litopenaeus vannamei and can cause huge economic loss in shrimp farming industry. The specific aim of this study was to develop a new sensitive real-time PCR method for the specific detection of shrimp IMNV. A real-time PCR assay with a pair of primers to specifically amplify a 101bp IMNV cDNA fragment and a corresponding TaqMan probe was developed, which shown to be specific for IMNV without cross reaction with DNA samples prepared from four other shrimp viruses including white spot syndrome virus (WSSV), hepatopancreatic parvovirus (HPV), monodon baculovirus (MBV), and infectious hypodermal and haematopoietic virus (IHHNV). The method could detect as low as one single copy of IMNV plasmid cDNA.
authors: College of Agriculture, Ludong University, Yantai, PR China.
authors: Hong-Ling
authors: HL
authors: Liu
authors: Dong-Chun
authors: DC
authors: Yan
authors: Hu-Shan
authors: HS
authors: Sun
authors: Yi-Yan
authors: YY
authors: Wang
authors: Lei
authors: L
authors: Wang
mesh_terms: D000818:Animals; D019198:Giardiavirus / Q000235:genetics / Q000302:isolation & purification*; D033561:Penaeidae / Q000821:virology*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity