title: Trypanosoma brucei Tb927.2.6100 is an essential protein associated with kinetoplast DNA.
abstract: The mitochondrial DNA of trypanosomatid protozoa consists of a complex, intercatenated network of tens of maxicircles and thousands of minicircles. This structure, called kinetoplast DNA (kDNA), requires numerous proteins and multiprotein complexes for replication, segregation, and transcription. In this study, we used a proteomic approach to identify proteins that are associated with the kDNA network. We identified a novel protein encoded by Tb927.2.6100 that was present in a fraction enriched for kDNA and colocalized the protein with kDNA by fluorescence microscopy. RNA interference (RNAi) knockdown of its expression resulted in a growth defect and changes in the proportion of kinetoplasts and nuclei in the cell population. RNAi also resulted in shrinkage and loss of the kinetoplasts, loss of maxicircle and minicircle components of kDNA at similar rates, and (perhaps secondarily) loss of edited and pre-edited mRNA. These results indicate that the Tb927.2.6100 protein is essential for the maintenance of kDNA.
authors: Seattle Biomedical Research Institute, Seattle, Washington, USA.
authors: Kirsten
authors: K
authors: Beck
authors: Nathalie
authors: N
authors: Acestor
authors: Anjelique
authors: A
authors: Schulfer
authors: Atashi
authors: A
authors: Anupama
authors: Jason
authors: J
authors: Carnes
authors: Aswini K
authors: AK
authors: Panigrahi
authors: Ken
authors: K
authors: Stuart
mesh_terms: D002846:Chromatography, Affinity; D018105:DNA, Kinetoplast / Q000378:metabolism*; D004272:DNA, Mitochondrial / Q000378:metabolism; D016133:Polymerase Chain Reaction; D021381:Protein Transport; D015800:Protozoan Proteins / Q000378:metabolism*; D034622:RNA Interference; D016053:RNA, Protozoan / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism; D014346:Trypanosoma brucei brucei / Q000166:cytology / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*
chemical_list: D018105:DNA, Kinetoplast; D004272:DNA, Mitochondrial; D015800:Protozoan Proteins; D016053:RNA, Protozoan
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