title: Drought stress acclimation imparts tolerance to Sclerotinia sclerotiorum and Pseudomonas syringae in Nicotiana benthamiana.
abstract: Acclimation of plants with an abiotic stress can impart tolerance to some biotic stresses. Such a priming response has not been widely studied. In particular, little is known about enhanced defense capacity of drought stress acclimated plants to fungal and bacterial pathogens. Here we show that prior drought acclimation in Nicotiana benthamiana plants imparts tolerance to necrotrophic fungus, Sclerotinia sclerotiorum, and also to hemi-biotrophic bacterial pathogen, Pseudomonas syringae pv. tabaci. S. sclerotiorum inoculation on N. benthamiana plants acclimated with drought stress lead to less disease-induced cell death compared to non-acclimated plants. Furthermore, inoculation of P. syringae pv. tabaci on N. benthamiana plants acclimated to moderate drought stress showed reduced disease symptoms. The levels of reactive oxygen species (ROS) in drought acclimated plants were highly correlated with disease resistance. Further, in planta growth of GFPuv expressing P. syringae pv. tabaci on plants pre-treated with methyl viologen showed complete inhibition of bacterial growth. Taken together, these experimental results suggested a role for ROS generated during drought acclimation in imparting tolerance against S. sclerotiorum and P. syringae pv. tabaci. We speculate that the generation of ROS during drought acclimation primed a defense response in plants that subsequently caused the tolerance against the pathogens tested.
authors: Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, OK 73401, USA. ksmysore@noble.org.
authors: Venkategowda
authors: V
authors: Ramegowda
authors: Muthappa
authors: M
authors: Senthil-Kumar
authors: Yasuhiro
authors: Y
authors: Ishiga
authors: Amita
authors: A
authors: Kaundal
authors: Makarla
authors: M
authors: Udayakumar
authors: Kirankumar S
authors: KS
authors: Mysore
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000064:Acclimatization / Q000187:drug effects / Q000235:genetics; D001203:Ascomycota / Q000187:drug effects / Q000502:physiology*; D016923:Cell Death / Q000187:drug effects; D055864:Droughts; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D018517:Plant Roots / Q000187:drug effects / Q000502:physiology; D018520:Plant Shoots / Q000187:drug effects / Q000502:physiology; D044224:Pseudomonas syringae / Q000187:drug effects / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics; D014026:Nicotiana / Q000166:cytology / Q000235:genetics / Q000382:microbiology* / Q000502:physiology*
chemical_list: D017382:Reactive Oxygen Species; D000040:Abscisic Acid
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