title: Bead assembly magnetorotation as a signal transduction method for protein detection.
abstract: This paper demonstrates a proof-of-principle for a new signal transduction method for protein detection called Bead Assembly Magnetorotation (BAM). BAM is based on using the target protein to mediate the formation of aptamer-coated 1 μm magnetic beads into a bead assembly, formed at the bottom of a 1 μL hanging droplet. The size, shape and fractal dimension of this bead assembly all depend on the protein concentration. The protein concentration can be measured in two ways: by magnetorotation, in which the rotational period of the assembly correlates with the protein concentration, or by fractal analysis. Additionally, a microscope-free magnetorotation detection method is introduced, based on a simple laser apparatus built from standard laboratory components. In this paper, we chose to focus on the protein thrombin, a popular choice for proof-of-principle work in this field.
authors: Department of Biomedical Engineering, University of Michigan, 2200 Bonisteel Blvd., Ann Arbor, MI 48109, USA.
authors: Ariel
authors: A
authors: Hecht
authors: Patrick
authors: P
authors: Commiskey
authors: Nicholas
authors: N
authors: Shah
authors: Raoul
authors: R
authors: Kopelman
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D017709:Fractals; D006801:Humans; D057230:Limit of Detection; D059346:Magnets / Q000737:chemistry*; D010316:Particle Size; D013917:Thrombin / Q000032:analysis*
chemical_list: D052157:Aptamers, Nucleotide; D013917:Thrombin
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