title: Ospapst1, a useful mutant for identifying seed purity and authenticity in hybrid rice.
abstract: The stability and completeness of male sterility is still a challenge in some male sterile rice lines, especially those of photoperiod/thermo-sensitive genic male sterility (P/TGMS). Leaf color marker is a widely practiced approach to reduce the impact of self-pollinated seeds of male sterile lines. The papst1 is a leaf color mutant. The newly emerged leaves of papst1 are chlorosis and have an impaired photosynthesis. But the other agronomic traits, such as germination rate, duration of maturation and seed weight, are not changed. The papst1/PAPST1 F₁ showed the wild-type leaf phenotype. The papst1/PAPST1 F₂ progenies displayed an approximately 3:1 segregation ratio of WT phenotype:mutant phenotype (72: 28, χ(2) = 0.48, p > 0.05), suggesting that papst1 mutant phenotype is caused by a single repressive gene. Map-based cloning and sequencing analysis revealed that a point mutation was occurred in Os01 g16040 (OsPAPST1). Given these results, the Ospapst1 mutant is a useful mutant for identifying seed purity and authenticity in hybrid rice.
authors: State Key Laboratory of Plant Physiology and Biochemistry; College of Life Science; Zhejiang University; Hangzhou, PR China.
authors: Qundan
authors: Q
authors: Lv
authors: Jiming
authors: J
authors: Xu
authors: Ping
authors: P
authors: Wu
mesh_terms: D060365:Chloroplast Proteins / Q000235:genetics*; D006824:Hybridization, Genetic; D024101:Mitochondrial Proteins / Q000235:genetics*; D033704:Nucleotide Transport Proteins / Q000235:genetics*; D012275:Oryza / Q000235:genetics*; D017354:Point Mutation; D012639:Seeds
chemical_list: D060365:Chloroplast Proteins; D024101:Mitochondrial Proteins; D033704:Nucleotide Transport Proteins
keywords: Ospapst1; hybrid rice; leaf color; seed purity
references: Cell Res. 2012 Apr;22(4):649-60
references: 22349461
references: Plant Physiol. 2013 Apr;161(4):2036-48
references: 23411694