title: Protective role of a methanolic extract of spinach (Spinacia oleracea L.) against Pb toxicity in wheat (Triticum aestivum L.) seedlings: beneficial effects for a plant of a nutraceutical used with animals.
abstract: Spinach extracts contain powerful natural antioxidants and have been used to improve the response of animal cells to various stress factors. The aim of the present study was to assess the effects of a methanolic extract of spinach (SE) used at two concentrations (21.7 and 217 ppm) on the growth, certain enzymes and antioxidant systems in wheat seedlings under lead stress. When wheat seedlings were grown for 7 days in a solution containing Pb(NO3)2 (3 mM), germination and growth were impaired, while signs of oxidative stress were observed. SE (217 ppm) pretreatment was able to protect seedlings from Pb toxicity by both reducing Pb uptake and Pb-induced oxidative stress. As a consequence, almost normal germination, elongation, biomass and α-amylase activity were restored by SE (217 ppm) pretreatment of wheat seedlings, in spite of the presence of Pb. Our results support the protective role and the antioxidant effect of SE against Pb. These results show an amazing similarity to the effects of SE in animals, which suggests that providing "nutraceuticals" to plants could improve their "health" status.
authors: PER-Centre des Etudes Environnementales Méditerranéennes, Equipe de recherche Biotechnologies et Génie des Biomolécules, Faculté des Sciences et Techniques, Université Abdelmalek Essaadi, BP 416, Tangier, Morocco.
authors: Mostafa
authors: M
authors: Lamhamdi
authors: Ahmed
authors: A
authors: Bakrim
authors: Noureddin
authors: N
authors: Bouayad
authors: Ahmed
authors: A
authors: Aarab
authors: René
authors: R
authors: Lafont
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D060387:Ascorbate Peroxidases / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D019587:Dietary Supplements; D018525:Germination / Q000187:drug effects; D007854:Lead / Q000633:toxicity*; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology*; D036226:Seedlings / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D012989:Soil Pollutants / Q000633:toxicity*; D018724:Spinacia oleracea / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D014908:Triticum / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D012989:Soil Pollutants; D007854:Lead; D008315:Malondialdehyde; D060387:Ascorbate Peroxidases; D002374:Catalase; D013482:Superoxide Dismutase
references: Anal Biochem. 1971 Nov;44(1):276-87
references: 4943714
references: J Biol Chem. 1952 Mar;195(1):133-40
references: 14938361
references: J Hazard Mater. 2012 Jun 15;219-220:1-12
references: 22502897
references: Toxicol Lett. 2001 Aug 6;123(1):1-10
references: 11514100
references: Plant Physiol. 1995 Mar;107(3):737-750
references: 12228398
references: Plant Physiol. 2000 Nov;124(3):1019-26
references: 11080279
references: Rev Environ Contam Toxicol. 2011;213:113-36
references: 21541849
references: Toxicol Pathol. 2000 Jul-Aug;28(4):588-600
references: 10930047
references: J Nutr. 2003 Nov;133(11):3584-7
references: 14608077
references: Plant Physiol Biochem. 2008 Oct;46(10):844-54
references: 18653353
references: C R Biol. 2011 Feb;334(2):118-26
references: 21333942
references: Environ Sci Technol. 1997 Apr;31(4):182A-6A
references: 21650618
references: Phytochemistry. 2001 Sep;58(1):143-52
references: 11524124
references: J Agric Food Chem. 2008 May 28;56(10):3532-7
references: 18444661
references: Pestic Biochem Physiol. 1996 Jun;55(2):109-21
references: 8980035
references: J Agric Food Chem. 1999 Jun;47(6):2213-7
references: 10794612
references: J Biol Chem. 1974 Nov 25;249(22):7130-9
references: 4436300
references: J Agric Food Chem. 2001 Jun;49(6):2767-73
references: 11409964
references: Saudi J Biol Sci. 2013 Jan;20(1):29-36
references: 23961216
references: Plant Physiol. 2006 Jun;141(2):391-6
references: 16760493
references: Environ Sci Pollut Res Int. 2013 Jul;20(7):4551-61
references: 23263755
references: Plant Physiol. 1977 May;59(5):1011-2
references: 16659940
references: Phytomedicine. 2004 Nov;11(7-8):607-15
references: 15636174
references: Can J Physiol Pharmacol. 2002 Dec;80(12):1187-94
references: 12564645
references: Rev Environ Contam Toxicol. 2008;196:73-93
references: 19025093
references: Anal Biochem. 1976 May 7;72:248-54
references: 942051
references: Guang Pu Xue Yu Guang Pu Fen Xi. 2003 Jun;23(3):583-6
references: 12953548
references: Insect Biochem Mol Biol. 2000 Mar;30(3):207-13
references: 10732988
references: Phytochemistry. 2004 Mar;65(6):711-20
references: 15016567