title: Tendon graft revitalization using adult anterior cruciate ligament (ACL)-derived CD34+ cell sheets for ACL reconstruction.
abstract: As a new strategy for enhancing recovery after anterior cruciate ligament (ACL) reconstruction, we developed a cell sheet wrapped graft using ACL-derived CD34+ cells. Our results from an ex vivo cell migration assay demonstrated gradual cell migration into the graft from the cell sheet, and an enzyme-linked immunosorbent assay indicated that the cell sheet cultures secreted a significantly greater amount of VEGF than typical monolayer cultures. In the in vivo study, the rats received one of three possible ACL reconstruction treatments; 1) a tendon graft wrapped in an ACL-derived CD34+ cell sheet, 2) a tendon graft and ACL-derived CD34+ cell injection, or 3) the control group that received just the tendon graft. The in vivo studies revealed that the ACL-derived CD34+ cell sheet wrapped grafts demonstrated a greater number of the cells derived from the cell sheets incorporated within the bone tunnel site and grafted tendon. We believe the incorporated CD34+ cells enhanced the healing of both the bone-tendon junction and the grafted tendon in the reconstructed rat ACLs by increasing proprioceptive recovery, graft maturation, and biomechanical strength. ACL-derived CD34+ cell sheet wrapped grafts could become a promising strategy to revitalize tendon autografts and recovery after ACL reconstruction resulting in superior and quicker recovery in patients requiring ACL reconstruction.
authors: Stem Cell Research Center, Department of Orthopaedic Surgery, University of Pittsburgh, Pittsburgh, PA 15219, USA.
authors: Yutaka
authors: Y
authors: Mifune
authors: Tomoyuki
authors: T
authors: Matsumoto
authors: Koji
authors: K
authors: Takayama
authors: Satoshi
authors: S
authors: Terada
authors: Naosumi
authors: N
authors: Sekiya
authors: Ryosuke
authors: R
authors: Kuroda
authors: Masahiro
authors: M
authors: Kurosaka
authors: Freddie H
authors: FH
authors: Fu
authors: Johnny
authors: J
authors: Huard
mesh_terms: D000328:Adult; D000818:Animals; D016118:Anterior Cruciate Ligament / Q000166:cytology* / Q000503:physiopathology / Q000637:transplantation*; D059549:Anterior Cruciate Ligament Reconstruction; D018952:Antigens, CD34 / Q000378:metabolism*; D001696:Biomechanical Phenomena; D001842:Bone and Bones / Q000473:pathology; D018929:Cell Culture Techniques / Q000379:methods*; D002465:Cell Movement; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D011434:Proprioception; D051381:Rats; D011923:Rats, Nude; D020127:Recovery of Function; D013710:Tendons / Q000473:pathology / Q000503:physiopathology / Q000637:transplantation*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014945:Wound Healing; D055815:Young Adult
chemical_list: D018952:Antigens, CD34; D042461:Vascular Endothelial Growth Factor A