title: Functional evaluation of iodoacetic acid induced photoreceptor degeneration in the cat.
abstract: Iodoacetic acid (IAA) has been applied to different species to acutely induce photoreceptor degeneration. The purpose of the present study was to use this toxin to thoroughly eliminate photoreceptors and induce complete blindness in the cat. IAA was delivered by single ear vein injection (20 mg kg(-1)). Six months after the IAA treatment, functional evaluations including pupillary light reflex (PLR), electroretinogram (ERG), visual behavior tests were performed. Morphological examinations were carried out after the functional evaluation. The present result shows that, six months after the IAA application, animals lost visual functions and became completely blind. High dose IAA application via ear vein delivery created an acute and reliable complete photoreceptor degeneration model in the cat. This model can be applied to genetic and cellular therapies for visual function restoration.
authors: Department of Anatomy/Embryology, School of Basic Medical Sciences, Peking University, Beijing 100191, China.
authors: Yan
authors: Y
authors: Nan
authors: Qin
authors: Q
authors: Zhang
authors: Chaoran
authors: C
authors: Ren
authors: Xin
authors: X
authors: Huang
authors: Jie
authors: J
authors: Gao
authors: Xiaoxin
authors: X
authors: Li
authors: Mingliang
authors: M
authors: Pu
mesh_terms: D000818:Animals; D001766:Blindness / Q000139:chemically induced / Q000503:physiopathology; D002415:Cats; D004195:Disease Models, Animal; D004596:Electroretinography; D005260:Female; D007275:Injections, Intravenous; D019807:Iodoacetic Acid / Q000008:administration & dosage / Q000633:toxicity*; D008297:Male; D020419:Photoreceptor Cells, Vertebrate / Q000187:drug effects* / Q000473:pathology; D012160:Retina / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology; D012162:Retinal Degeneration / Q000139:chemically induced / Q000503:physiopathology*; D013997:Time Factors; D014785:Vision, Ocular / Q000187:drug effects / Q000502:physiology
chemical_list: D019807:Iodoacetic Acid