title: Comparison of keratocyte density after femtosecond laser vs mechanical microkeratome from 3 months up to 5 years after LASIK.
abstract: BACKGROUND
  To compare keratocyte density after mechanical microkeratome LASIK (MK-LASIK) and femtosecond-laser assisted LASIK (FS-LASIK).
  
  
  METHODS
  We performed a prospective study of myopic patients that underwent MK-LASIK or FS-LASIK. We measured keratocyte density 3 and 15 months, and 3-5 years after the surgery using confocal microscopy, and compared them with healthy, non-operated corneas.
  
  
  RESULTS
  Thirty-one eyes were included in the FS-LASIK group, 30 in the MK-LASIK group and 28 in the control group. Three months postoperatively, there was an increase in the keratocyte population of the whole cornea, mainly due to the mid and posterior stromal layers, in both treatment groups. It was also increased in the stromal bed after MK-LASIK, but not after FS-LASIK. In both groups, this was followed by a normalisation and stabilisation of cell density in those deeper layers 15 months after the surgery. Keratocyte density in the flap and stromal bed was decreased 15 months after FS- and MK-LASIK compared to 3 months postoperatively and compared to controls. It seemed to remain stable thereafter. In spite of this decrease, the average cell density throughout the cornea was not decreased compared to controls at any time point.
  
  
  CONCLUSIONS
  We found a reorganization of keratocytes density after LASIK, with an initial increase, followed by a decrease in the stromal flap and stromal bed 15 months postoperatively, and stable from then onwards, but still maintaining normal average densities in the total cornea. There were no differences between MK- and FS-LASIK.
authors: Vissum Corporation, C/ Santa Hortensia 58, 28002, Madrid, Spain, pilarcanadas.s@gmail.com.
authors: Pilar
authors: P
authors: Cañadas
authors: Laura
authors: L
authors: de Benito-Llopis
authors: José Luis
authors: JL
authors: Hernández-Verdejo
authors: Miguel A
authors: MA
authors: Teus
mesh_terms: D000328:Adult; D002452:Cell Count; D060527:Corneal Keratocytes / Q000473:pathology*; D063171:Corneal Pachymetry; D003319:Corneal Stroma / Q000473:pathology; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D020731:Keratomileusis, Laser In Situ / Q000379:methods*; D054018:Lasers, Excimer / Q000627:therapeutic use*; D008297:Male; D018613:Microscopy, Confocal; D009216:Myopia / Q000503:physiopathology / Q000601:surgery*; D011446:Prospective Studies; D013524:Surgical Flaps / Q000473:pathology; D014792:Visual Acuity / Q000502:physiology; D055815:Young Adult
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