title: Combined zoledronic acid and meloxicam reduced bone loss and tumour growth in an orthotopic mouse model of bone-invasive oral squamous cell carcinoma.
abstract: Oral squamous cell carcinoma (OSCC) is common in cats and humans and invades oral bone. We hypothesized that the cyclooxygenase (COX)-2 inhibitor, meloxicam, with the bisphosphonate, zoledronic acid (ZOL), would inhibit tumour growth, osteolysis and invasion in feline OSCC xenografts in mice. Human and feline OSCC cell lines expressed COX-1 and COX-2 and the SCCF2 cells had increased COX-2 mRNA expression with bone conditioned medium. Luciferase-expressing feline SCCF2Luc cells were injected beneath the perimaxillary gingiva and mice were treated with 0.1 mg kg(-1) ZOL twice weekly, 0.3 mg kg(-1) meloxicam daily, combined ZOL and meloxicam, or vehicle. ZOL inhibited osteoclastic bone resorption at the tumour-bone interface. Meloxicam was more effective than ZOL at reducing xenograft growth but did not affect osteoclastic bone resorption. Although a synergistic effect of combined ZOL and meloxicam was not observed, combination therapy was well-tolerated and may be useful in the clinical management of bone-invasive feline OSCC.
authors: Department of Veterinary Biosciences, College of Veterinary Medicine, Columbus, OH, USA.
authors: C K
authors: CK
authors: Martin
authors: Department of Veterinary Biosciences, College of Veterinary Medicine, Columbus, OH, USA.
authors: W P
authors: WP
authors: Dirksen
authors: Small Animal Imaging Center Shared Resource, Comprehensive Cancer Center, The Ohio State University, Columbus, OH, USA.
authors: M M
authors: MM
authors: Carlton
authors: Department of Veterinary Biosciences, College of Veterinary Medicine, Columbus, OH, USA.
authors: L G
authors: LG
authors: Lanigan
authors: Department of Veterinary Biosciences, College of Veterinary Medicine, Columbus, OH, USA.
authors: S P
authors: SP
authors: Pillai
authors: Department of Veterinary Biosciences, College of Veterinary Medicine, Columbus, OH, USA.
authors: J L
authors: JL
authors: Werbeck
authors: Department of Veterinary Biosciences, College of Veterinary Medicine, Columbus, OH, USA.
authors: J K
authors: JK
authors: Simmons
authors: Department of Veterinary Biosciences, College of Veterinary Medicine, Columbus, OH, USA.
authors: B E
authors: BE
authors: Hildreth
authors: Department of Veterinary Biosciences, College of Veterinary Medicine, Columbus, OH, USA.
authors: C A
authors: CA
authors: London
authors: Department of Veterinary Biosciences, College of Veterinary Medicine, Columbus, OH, USA.
authors: R E
authors: RE
authors: Toribio
authors: Department of Veterinary Biosciences, College of Veterinary Medicine, Columbus, OH, USA.
authors: T J
authors: TJ
authors: Rosol
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use; D050071:Bone Density Conservation Agents / Q000627:therapeutic use*; D001859:Bone Neoplasms / Q000188:drug therapy* / Q000473:pathology / Q000556:secondary / Q000662:veterinary; D001862:Bone Resorption / Q000188:drug therapy / Q000517:prevention & control; D002371:Cat Diseases / Q000188:drug therapy; D002415:Cats; D045744:Cell Line, Tumor; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D016861:Cyclooxygenase Inhibitors / Q000627:therapeutic use; D004164:Diphosphonates / Q000627:therapeutic use*; D004195:Disease Models, Animal; D064593:Heterografts; D006801:Humans; D007093:Imidazoles / Q000627:therapeutic use*; D008297:Male; D000077239:Meloxicam; D051379:Mice; D008819:Mice, Nude; D009062:Mouth Neoplasms / Q000188:drug therapy* / Q000473:pathology / Q000662:veterinary; D018307:Neoplasms, Squamous Cell / Q000188:drug therapy* / Q000556:secondary / Q000662:veterinary; D012333:RNA, Messenger; D011897:Random Allocation; D060888:Real-Time Polymerase Chain Reaction; D013843:Thiazines / Q000627:therapeutic use*; D013844:Thiazoles / Q000627:therapeutic use*; D016896:Treatment Outcome; D000077211:Zoledronic Acid
chemical_list: D050071:Bone Density Conservation Agents; D016861:Cyclooxygenase Inhibitors; D004164:Diphosphonates; D007093:Imidazoles; D012333:RNA, Messenger; D013843:Thiazines; D013844:Thiazoles; D000077211:Zoledronic Acid; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; C496511:PTGS1 protein, human; C496540:PTGS2 protein, human; D000077239:Meloxicam
keywords: bone invasion; bone resorption; feline; meloxicam; oral squamous cell carcinoma; zoledronic acid
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