title: Halplotypes of the ovine ADRB3 gene (ADRB3) and their association with post-weaning growth in New Zealand Suffolk sheep.
abstract: The β3-adrenergic receptor (ADRB3) regulates thermogenesis and lipolysis in brown and white adipose tissue. Previously, sixteen ovine ADRB3 haplotypes have been defined. In this study, the relationship between these ADRB3 haplotypes and variation in post-weaning growth was investigated in 797 New Zealand Suffolk lambs from 38 sire lines and eight studs, using PCR-SSCP and General Linear Mixed-effects Models. Seven haplotypes were found in these sheep and they comprised five previously reported intron sequences and four previously reported 3'UT sequences. The frequencies of the various diplotypes ranged from 0.1 to 17.6% and individual haplotypes from 0.8 to 32.5%. The presence of haplotype A-b was associated with a decreased weaning-weight (P=0.001). Sheep with the B-c/F-e diplotype had a higher mean weaning-weight than those with A-b/B-c or A-b/E-e (P<0.05). The presence of C-a was found to be associated with increased post-weaning growth (P=0.008), while the presence of B-c was associated with decreased post-weaning growth (P=0.005). Sheep with A-b/C-a had higher mean post-weaning growth than those with A-b/A-b, A-b/B-c, B-c/B-c, B-c/E-e or B-c/F-e (P<0.05). Sheep with B-c/B-c had lower mean post-weaning growth than those with A-b/C-a or B-c/C-a (P<0.05). Additive effects for the different forms of the B-c haplotypes on post-weaning growth were identified. The effects of the ovine ADRB3 haplotypes on weaning-weight and post-weaning growth confirm that they could be used as a candidate gene-marker for improving sheep growth.
authors: Gansu Key Laboratory of Herbivorous Animal Biotechnology, Gansu Agricultural University, Lanzhou, China.
authors: Guo
authors: G
authors: Yang
authors: Huitong
authors: H
authors: Zhou
authors: Rachel H
authors: RH
authors: Forrest
authors: Qian
authors: Q
authors: Fang
authors: Yuzhu
authors: Y
authors: Luo
authors: Jon G H
authors: JG
authors: Hickford
mesh_terms: D000818:Animals; D001835:Body Weight / Q000502:physiology; D001947:Breeding / Q000379:methods; D006239:Haplotypes / Q000235:genetics*; D016014:Linear Models; D009520:New Zealand; D010641:Phenotype; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D022702:Receptors, Adrenergic, beta-3 / Q000235:genetics*; D012756:Sheep / Q000235:genetics* / Q000254:growth & development*; D014886:Weaning
chemical_list: D022702:Receptors, Adrenergic, beta-3
references: J Anim Sci. 2007 Nov;85(11):2801-6
references: 17644783
references: Biochem Genet. 2009 Aug;47(7-8):498-502
references: 19507019
references: Science. 2007 Jan 26;315(5811):525-8
references: 17185560
references: Anim Genet. 2009 Apr;40(2):251
references: 19032697
references: Anal Biochem. 2006 Jul 1;354(1):159-61
references: 16643836
references: Diabetes. 1999 Jan;48(1):117-20
references: 9892231
references: N Engl J Med. 1995 Aug 10;333(6):352-4
references: 7609752
references: DNA Cell Biol. 2011 Jul;30(7):445-8
references: 21348572
references: PLoS Biol. 2012 Feb;10(2):e1001258
references: 22346734
references: Anim Genet. 2003 Feb;34(1):19-25
references: 12580782
references: Anal Biochem. 2009 Feb 1;385(1):174-5
references: 19013423
references: Diabetologia. 1997 Apr;40(4):469-72
references: 9112025
references: Mol Biol Rep. 2012 Aug;39(8):8395-403
references: 22711302
references: Diabetologia. 1981 Mar;20(Suppl 1):362-365
references: 27942823
references: Anim Genet. 2009 Dec;40(6):958-62
references: 19531115
references: Hum Hered. 1999 Mar;49(2):112-8
references: 10077733
references: Anim Genet. 2006 Oct;37(5):465-8
references: 16978175
references: Pharmacogenomics. 2007 Aug;8(8):1075-80
references: 17716239
references: Eur J Biochem. 1997 Aug 1;247(3):1174-9
references: 9288945
references: Mol Cell Probes. 2008 Feb;22(1):69-70
references: 17936579
references: Reprod Nutr Dev. 1996;36(4):391-7
references: 8878356