title: Highly sensitive recognition of Pb(2+) using Pb(2+) triggered exonuclease aided DNA recycling.
abstract: Here, we have demonstrated an ultra-high sensitive detection platform with the detection limit of 5pM for an environmental toxin-Pb(2+). We designed a Pb(2+) triggered exonuclease aided DNA recycling system to improve the detection sensitivity. In our system, a Pb(2+) dependent 8-17 DNAzyme and its substrate were used to form hybridization duplex. In the presence of Pb(2+), the substrate was cleaved and disassociated from the duplex. Then, the released 8-17 DNAzyme was used as a target of the exonuclease aided DNA recycling system which can amplify the fluorescence signal by recycling the 8-17 DNAzyme continuously. Then, the sensitive Pb(2+) detection are accomplished and the detection limit of Pb(2+) was down to 5pM which is about 1000 times lower than the traditional detection method based on the 8-17 DNAzyme.
authors: School of Chemistry and Materials Science, Ludong University, Yantai, 264025 China. xuhui235@yahoo.com.cn
authors: Hui
authors: H
authors: Xu
authors: Pingping
authors: P
authors: Xu
authors: Shanmin
authors: S
authors: Gao
authors: Shengxiao
authors: S
authors: Zhang
authors: Xingchun
authors: X
authors: Zhao
authors: Chunhai
authors: C
authors: Fan
authors: Xiaolei
authors: X
authors: Zuo
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D021881:DNA, Catalytic / Q000737:chemistry*; D005090:Exodeoxyribonucleases / Q000737:chemistry*; D005453:Fluorescence; D015386:Hazardous Substances / Q000302:isolation & purification* / Q000633:toxicity; D006801:Humans; D007854:Lead / Q000302:isolation & purification* / Q000633:toxicity; D057230:Limit of Detection; D009693:Nucleic Acid Hybridization
chemical_list: D021881:DNA, Catalytic; D015386:Hazardous Substances; D007854:Lead; D005090:Exodeoxyribonucleases