title: Identification of bacterial protein O-oligosaccharyltransferases and their glycoprotein substrates.
abstract: O-glycosylation of proteins in Neisseria meningitidis is catalyzed by PglL, which belongs to a protein family including WaaL O-antigen ligases. We developed two hidden Markov models that identify 31 novel candidate PglL homologs in diverse bacterial species, and describe several conserved sequence and structural features. Most of these genes are adjacent to possible novel target proteins for glycosylation. We show that in the general glycosylation system of N. meningitidis, efficient glycosylation of additional protein substrates requires local structural similarity to the pilin acceptor site. For some Neisserial PglL substrates identified by sensitive analytical approaches, only a small fraction of the total protein pool is modified in the native organism, whereas others are completely glycosylated. Our results show that bacterial protein O-glycosylation is common, and that substrate selection in the general Neisserial system is dominated by recognition of structural homology.
authors: School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Queensland, Australia.
authors: Benjamin L
authors: BL
authors: Schulz
authors: Freda E C
authors: FE
authors: Jen
authors: Peter M
authors: PM
authors: Power
authors: Christopher E
authors: CE
authors: Jones
authors: Kate L
authors: KL
authors: Fox
authors: Shan C
authors: SC
authors: Ku
authors: Joanne T
authors: JT
authors: Blanchfield
authors: Michael P
authors: MP
authors: Jennings
mesh_terms: D000150:Acinetobacter / Q000235:genetics / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006023:Glycoproteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006031:Glycosylation; D016695:Glycosyltransferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008390:Markov Chains; D008969:Molecular Sequence Data; D009345:Neisseria meningitidis / Q000737:chemistry* / Q000201:enzymology / Q000235:genetics; D019081:O Antigens / Q000737:chemistry*; D011499:Protein Processing, Post-Translational; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D006023:Glycoproteins; D019081:O Antigens; D011994:Recombinant Proteins; D016695:Glycosyltransferases
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