title: Protein production from the structural genomics perspective: achievements and future needs.
abstract: Despite a multitude of recent technical breakthroughs speeding high-resolution structural analysis of biological macromolecules, production of sufficient quantities of well-behaved, active protein continues to represent the rate-limiting step in many structure determination efforts. These challenges are only amplified when considered in the context of ongoing structural genomics efforts, which are now contending with multi-domain eukaryotic proteins, secreted proteins, and ever-larger macromolecular assemblies. Exciting new developments in eukaryotic expression platforms, including insect and mammalian-based systems, promise enhanced opportunities for structural approaches to some of the most important biological problems. Development and implementation of automated eukaryotic expression techniques promises to significantly improve production of materials for structural, functional, and biomedical research applications.
authors: Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, United States. steve.almo@einstein.yu.edu
authors: Steven C
authors: SC
authors: Almo
authors: Scott J
authors: SJ
authors: Garforth
authors: Brandan S
authors: BS
authors: Hillerich
authors: James D
authors: JD
authors: Love
authors: Ronald D
authors: RD
authors: Seidel
authors: Stephen K
authors: SK
authors: Burley
mesh_terms: D000818:Animals; D019295:Computational Biology; D005057:Eukaryotic Cells / Q000737:chemistry / Q000378:metabolism*; D015870:Gene Expression; D023281:Genomics; D057166:High-Throughput Screening Assays; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification
chemical_list: D011994:Recombinant Proteins
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