title: Rapid and sensitive detection of maize chlorotic mottle virus using surface plasmon resonance-based biosensor.
abstract: We report a biosensor based on surface plasmon resonance (SPR) for the selective detection of maize chlorotic mottle virus (MCMV). 11-Mercaptoundecanoic acid was applied on a gold surface to form a self-assembled monolayer, and a layer of anti-MCMV antibody was crosslinked on the surface for specific recognition of MCMV. The effects of coupling reaction time and antibody concentration on detection sensitivity were studied. The coverage mass change is a function of the concentration of MCMV with a dynamic range from 1 to 1000 ppb. The detection limit is approximately 1 ppb, which is approximately two orders of magnitude higher than that of the existing enzyme-linked immunosorbent assay (ELISA) method. The developed SPR sensor showed highly specific recognition for both purified MCMV and crude extracts from real-world samples.
authors: Institute of Plant Quarantine, Chinese Academy of Inspection and Quarantine, Beijing 100029, People's Republic of China.
authors: Chang
authors: C
authors: Zeng
authors: Xin
authors: X
authors: Huang
authors: Jiangmin
authors: J
authors: Xu
authors: Guifen
authors: G
authors: Li
authors: Jie
authors: J
authors: Ma
authors: Hai-Feng
authors: HF
authors: Ji
authors: Shuifang
authors: S
authors: Zhu
authors: Hongjun
authors: H
authors: Chen
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D005227:Fatty Acids / Q000032:analysis*; D012680:Sensitivity and Specificity; D013438:Sulfhydryl Compounds / Q000032:analysis*; D020349:Surface Plasmon Resonance / Q000379:methods*; D019183:Tombusviridae / Q000302:isolation & purification*; D003313:Zea mays / Q000821:virology*
chemical_list: C505222:11-mercaptoundecanoic acid; D005227:Fatty Acids; D013438:Sulfhydryl Compounds
keywords: Biosensor; Maize chlorotic mottle virus; Surface plasmon resonance; Virus detection