title: Interleukin-10 gene promoter polymorphisms are associated with cyclosporin A-induced gingival overgrowth in renal transplant patients.
abstract: OBJECTIVE
  Interleukin-10 (IL-10) is an anti-inflammatory cytokine whose genetic polymorphisms are associated with the production of IL-10 and the susceptibility to periodontal diseases. The aim of this study was to investigate the possible association of IL-10 single nucleotide polymorphisms (SNPs) and cyclosporin A (CsA)-induced gingival overgrowth (GO) in renal transplant patients in a Chinese population, taking into account subgingival microbiota as additional variables.
  
  
  METHODS
  A total of 202 patients were dichotomized into two groups: 122 with GO and 80 without GO. The IL-10-1082 SNP, -819 SNP and -592 SNP were measured using an allele-specific PCR method. The levels of subgingival bacteria were measured by real-time PCR. Genotype and allele frequencies were analyzed using the Chi-square test and logistic regression analysis.
  
  
  RESULTS
  The frequency of IL-10-819TT (-592AA) genotype was statistically higher in patients with GO than that in patients without GO (P<0.05). Multiple logistic regression analysis demonstrated that the prevalence of GO is not dependent on age, gender, and pharmacological variables, being significantly associated with the carriers of ATA haplotype (OR=2.425, 95%CI=1.214-4.845, P=0.012). Moreover, ATA positive carriers in the GO group presented significantly higher levels of Porphyromonas gingivalis and Treponema denticola than those negative carriers.
  
  
  CONCLUSIONS
  Our results show that IL-10-819TT (-592AA) genotype and ATA halpotype are associated with susceptibility to CsA-induced GO. Meanwhile, ATA haplotype is associated with a higher detection of P. gingivalis and T. denticola in GO patients, and may increase the risk of developing GO.
authors: Department of Stomatology, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai, China.
authors: Yixi
authors: Y
authors: Luo
authors: Yiming
authors: Y
authors: Gong
authors: Youcheng
authors: Y
authors: Yu
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D003430:Cross-Sectional Studies; D016572:Cyclosporine / Q000009:adverse effects*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D005881:Gingiva / Q000187:drug effects / Q000382:microbiology*; D019214:Gingival Overgrowth / Q000139:chemically induced* / Q000235:genetics; D006239:Haplotypes; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D016753:Interleukin-10; D016572:Cyclosporine
keywords: Cyclosporin A; Cytokines; Gingival overgrowth; Interleukin-10; Periodontal pathogens; Polymorphism