title: Molecular diversity of tuliposide A-converting enzyme in the tulip.
abstract: Tuliposide A-converting enzyme (TCEA) catalyzes the conversion of 6-tuliposide A to its lactonized aglycon, tulipalin A, in the tulip (Tulipa gesneriana). The TgTCEA gene, isolated previously from petals, was transcribed in all tulip tissues but not in the bulbs despite the presence of TCEA activity, which allowed prediction of the presence of a TgTCEA isozyme gene preferentially expressed in the bulbs. Here, the TgTCEA-b gene, the TgTCEA homolog, was identified in bulbs. TgTCEA-b polypeptides showed approximately 77% identity to the petal TgTCEA. Functional characterization of the recombinant enzyme verified that TgTCEA-b encoded the TCEA. Moreover, the TgTCEA-b was found to be localized to plastids, as found for the petal TgTCEA. Transcript analysis revealed that TgTCEA-b was functionally transcribed in the bulb scales, unlike the TgTCEA gene, whose transcripts were absent there. In contrast, TgTCEA-b transcripts were in the minority in other tissues where TgTCEA transcripts were dominant, indicating a tissue preference for the transcription of those isozyme genes.
authors: Biotechnology Research Center, Toyama Prefectural University, Kurokawa, Imizu, Toyama, Japan. tnomura@pu-toyama.ac.jp
authors: Taiji
authors: T
authors: Nomura
authors: Aya
authors: A
authors: Tsuchigami
authors: Shinjiro
authors: S
authors: Ogita
authors: Yasuo
authors: Y
authors: Kato
mesh_terms: D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D006027:Glycosides / Q000378:metabolism*; D042541:Intracellular Space / Q000201:enzymology; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D012978:Sodium Oxybate / Q000031:analogs & derivatives* / Q000378:metabolism; D031447:Tulipa / Q000166:cytology / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D006027:Glycosides; D012333:RNA, Messenger; C062516:tuliposide A; D012978:Sodium Oxybate