title: Effect of micro-vibration culture system on embryo development.
abstract: OBJECTIVE
  Micro-vibration culture system was examined to determine the effects on mouse and human embryo development and possible improvement of clinical outcomes in poor responders.
  
  
  METHODS
  The embryonic development rates and cell numbers of blastocysts were compared between a static culture group (n = 178) and a micro-vibration culture group (n = 181) in mice. The embryonic development rates and clinical results were compared between a static culture group (n = 159 cycles) and a micro-vibration culture group (n = 166 cycles) in poor responders. A micro-vibrator was set at a frequency of 42 Hz, 5 s/60 min duration for mouse and human embryo development.
  
  
  RESULTS
  The embryonic development rate was significantly improved in the micro-vibration culture group in mice (p < 0.05). The cell numbers of mouse blastocysts were significantly higher in the micro-vibration group than in the static culture group (p < 0.05). In the poor responders, the rate of high grade embryos was not significantly improved in the micro-vibration culture group on day 3. However, the optimal embryonic development rate on day 5 was improved in the micro-vibration group, and the total pregnancy rate and implantation rate were significantly higher in the micro-vibration group than in the static culture group (p < 0.05).
  
  
  CONCLUSIONS
  Micro-vibration culture methods have a beneficial effect on embryonic development in mouse embryos. In poor responders, the embryo development rate was improved to a limited extent under the micro-vibration culture conditions, but the clinical results were significantly improved.
authors: Department of Anatomy, School of Medicine, Kangwon National University, Chuncheon, 200-701, Korea. geaher@mariababy.com
authors: Yong Soo
authors: YS
authors: Hur
authors: Jeong Hyun
authors: JH
authors: Park
authors: Eun Kyung
authors: EK
authors: Ryu
authors: Sung Jin
authors: SJ
authors: Park
authors: Jun Ho
authors: JH
authors: Lee
authors: Soo Hee
authors: SH
authors: Lee
authors: Jung
authors: J
authors: Yoon
authors: San Hyun
authors: SH
authors: Yoon
authors: Chang Young
authors: CY
authors: Hur
authors: Won Don
authors: WD
authors: Lee
authors: Jin Ho
authors: JH
authors: Lim
mesh_terms: D000328:Adult; D000818:Animals; D001755:Blastocyst / Q000166:cytology*; D003470:Culture Media; D046149:Embryo Culture Techniques / Q000379:methods*; D010064:Embryo Implantation; D004624:Embryo Transfer; D047108:Embryonic Development; D005260:Female; D006801:Humans; D051379:Mice; D010062:Ovulation Induction; D011247:Pregnancy; D018873:Pregnancy Rate; D014732:Vibration
chemical_list: D003470:Culture Media
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