title: Quantitative MUC5AC and MUC6 mucin estimations in gastric mucus by a least-squares minimization method.
abstract: We have determined the molar proportions of the MUC5AC and MUC6 mucus glycoproteins (mucins) in mucus from the normal and pathological human gastric antrum using a least-squares minimization analysis applied to amino acid compositions. We noted that the content of MUC5AC mucin in mucus from individuals without gastroduodenal disease was very high, suggesting that the integrity and barrier properties of the adherent gastric mucus layer are normally maintained by building-block structures formed from this mucin alone. We observed that the molar content of MUC6 mucin doubled (without significance) in mucus from patients with duodenal ulcer, and increased five times (with high significance) in mucus from patients with gastric ulcer, when compared with that in mucus from individuals without gastroduodenal disease.
authors: Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, London WC1H 9JB, UK. j.squire@imperial.ac.uk
authors: John M
authors: JM
authors: Squire
authors: Miguel J
authors: MJ
authors: Guerreiro
authors: Ramon L
authors: RL
authors: Sidebotham
authors: Celso A
authors: CA
authors: Reis
authors: Julian
authors: J
authors: Wiseman
authors: Pradeep K
authors: PK
authors: Luther
mesh_terms: D000328:Adult; D000368:Aged; D004381:Duodenal Ulcer / Q000378:metabolism*; D006801:Humans; D008875:Middle Aged; D055271:Mucin 5AC / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D055272:Mucin-6 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D009093:Mucus / Q000737:chemistry*; D013276:Stomach Ulcer / Q000378:metabolism*
chemical_list: C524678:MUC5AC protein, human; C524734:MUC6 protein, human; D055271:Mucin 5AC; D055272:Mucin-6
keywords: Duodenal ulcer; Gastric ulcer; Helicobacter pylori; MUC5AC mucin; MUC6 mucin; Protein quantification