title: [Expressions of COX-2, PKC-α and miR-101 in gastric cancer and their correlations].
abstract: OBJECTIVE
  To investigate the expressions of miR-101, protein kinase C-α (PKC-α), and cyclooxygenase-2 (COX-2) in gastric cancer (GC) tissue and their correlations.
  
  
  METHODS
  RT-qPCR was used to examine miR-101 expression and Western blotting employed to detect PKC-α and COX-2 expressions in 57 cases of gastric cancer tissues and paired normal gastric mucosal tissues.
  
  
  RESULTS
  The gastric cancer tissues showed a significantly lower miR-101 expression (Z=6.102, P<0.05) but significantly higher expressions of COX-2 (Z=14.436, P<0.05) and PKC-α (Z=6.955, P<0.05) than the normal gastric tissues. The expression of COX-2 protein was significantly correlated with the degree of differentiation, invasion depth, lymph node metastasis and TNM stage (P<0.05); PKC-α protein expression was associated with lymph node metastasis and TNM stage (P<0.05). PKC-α expression was positively correlated (r=0.531, P<0.05) and miR-101 expression negatively correlated (r=-0.627, P<0.05) with COX-2 expression in gastric cancer tissues.
  
  
  CONCLUSIONS
  miR-101, PKC-α and COX-2 all play a role in the tumorigenesis and progression of gastric cancer. miR-101 and PKC-α might be new potential therapeutic targets for inhibiting COX-2 in gastric cancer.
authors: Department of General Surgery, Second Affiliated Hospital of Xi'an Jiaotong University College of Medicine, China. outhome.sun@stu.xjtu.edu.cn
authors: Haibing
authors: H
authors: Sun
authors: Yongchang
authors: Y
authors: Wei
authors: Honglei
authors: H
authors: Tu
authors: Ning
authors: N
authors: DU
authors: Yang
authors: Y
authors: Zhao
authors: Lijuan
authors: L
authors: Hu
authors: Hong
authors: H
authors: Ren
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000378:metabolism*; D008875:Middle Aged; D009367:Neoplasm Staging; D051571:Protein Kinase C-alpha / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: C534072:MIRN101 microRNA, human; D035683:MicroRNAs; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human; C496628:PRKCA protein, human; D051571:Protein Kinase C-alpha