title: Development of a new duplex real-time polymerase chain reaction assay for detection of dicer in G. gallus.
abstract: Recently, there has been a growing body of evidence showing that cellular microRNAs (miRNAs) are involved in virus-host interactions. Numerous studies have focused on analyses of the expression profiles of cellular miRNAs, but the expression patterns of Dicer, which is responsible for the generation of miRNAs, have only rarely been explored in Gallus gallus. We developed a duplex realtime reverse transcriptase polymerase chain reaction (RTPCR) assay for the relative quantification of the mRNAs of Dicer and beta-actin in G. gallus. To apply this method, the expression of Dicer in avian cells after infection with avian leukosis virus subgroup J (ALV-J) was detected using our established duplex real-time RT-PCR. The duplex realtime RT-PCR assay is sufficiently sensitive, specific, accurate, reproducible, and cost-effective for the detection of Dicer in G. gallus. Furthermore, this study, for the first time, demonstrated that ALV-J can induce differential expression of Dicer mRNA in the ALV-J-infected cells.
authors: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
authors: Xiaolin
authors: X
authors: Ji
authors: Qi
authors: Q
authors: Wang
authors: Yulong
authors: Y
authors: Gao
authors: Yongqiang
authors: Y
authors: Wang
authors: Liting
authors: L
authors: Qin
authors: Xiaole
authors: X
authors: Qi
authors: Honglei
authors: H
authors: Gao
authors: Xiaomei
authors: X
authors: Wang
mesh_terms: D000818:Animals; D001353:Avian Leukosis / Q000821:virology; D001354:Avian Leukosis Virus / Q000502:physiology; D002645:Chickens / Q000235:genetics* / Q000378:metabolism / Q000821:virology; D011201:Poultry Diseases / Q000821:virology*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D043244:Ribonuclease III / Q000235:genetics* / Q000378:metabolism
chemical_list: D043244:Ribonuclease III