title: Lentiviral vector-mediated siRNA knockdown of c-MYC: cell growth inhibition and cell cycle arrest at G2/M phase in Jijoye cells.
abstract: Inhibition of c-MYC has been considered as a potential therapy for lymphoma treatment. We explored a lentiviral vector-mediated small interfering RNA (siRNA) expression vector to stably reduce c-MYC expression in B cell line Jijoye cells and investigated the effects of c-MYC downregulation on cell growth, cell cycle, and apoptosis in vitro. The expression of c-MYC mRNA and protein levels were inhibited significantly by c-MYC siRNA. The c-MYC downregulation resulted in the inhibition of cell proliferation and cell cycle arrest at G2/M phase, which was associated with decreased expression of cyclin B and cyclin-dependent kinase 1 (CDK1) and increased expression of CDK inhibitor p21 proteins. In addition, downregulation of c-MYC induced cell apoptosis characterized by DNA fragmentation and caspase-3 activation. Taken together, these results suggest that lentiviral vector-mediated siRNA for c-MYC may be a promising approach for targeting c-MYC in the treatment of Burkitt lymphoma.
authors: Department of Pediatric Hematology, Affiliated Hospital of Qingdao University Medical College, 16 Jiangsu Road, Qingdao, 266001 Shandong, China. aiqinsong@yahoo.cn
authors: Aiqin
authors: A
authors: Song
authors: Junli
authors: J
authors: Ye
authors: Kunpeng
authors: K
authors: Zhang
authors: Lirong
authors: L
authors: Sun
authors: Yanxia
authors: Y
authors: Zhao
authors: Hongsheng
authors: H
authors: Yu
mesh_terms: D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D002453:Cell Cycle; D059447:Cell Cycle Checkpoints; D002455:Cell Division; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D016195:G2 Phase; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D005822:Genetic Vectors; D006801:Humans; D016086:Lentivirus / Q000235:genetics*; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism*
chemical_list: D016271:Proto-Oncogene Proteins c-myc; D034741:RNA, Small Interfering; C505407:CASP3 protein, human; D053148:Caspase 3