title: The protective effects of piceatannol from passion fruit (Passiflora edulis) seeds in UVB-irradiated keratinocytes.
abstract: The use of naturally occurring botanicals with substantial antioxidant activity to prevent photoageing is receiving increasing attention. We have previously identified piceatannol and scirpusin B, which is a dimer of piceatannol, as strong antioxidants that are present in passion fruit (Passiflora edulis) seeds. In the present study, the effects of passion fruit seed extract, piceatannol, and scirpusin B on human keratinocytes were investigated. The passion fruit seed extract and piceatannol upregulated the glutathione (GSH) levels in keratinocytes in a dose-dependent manner, indicating that piceatannol is an active component of the passion fruit seed extract in keratinocytes. The pretreatment with piceatannol also suppressed the UVB-induced generation of reactive oxygen species (ROS) in the keratinocytes. In addition, the transfer of the medium from the UVB-irradiated keratinocytes to non-irradiated fibroblasts enhanced matrix-metalloproteinase (MMP)-1 activity, and this MMP-1 induction was reduced when the keratinocytes were pretreated with piceatannol. These results suggest that piceatannol attenuates the UVB-induced activity of MMP-1 along with a reduction of ROS generation in keratinocytes. Thus, piceatannol and passion fruit seed extract containing high amounts of piceatannol are potential anti-photoageing cosmetic ingredients.
authors: Health Care Division, Morinaga & Co, Ltd, Yokohama, Kanagawa 230–8504, Japan. h-uchida-ji@morinaga.co.jp
authors: Hiroko
authors: H
authors: Maruki-Uchida
authors: Ikuko
authors: I
authors: Kurita
authors: Kenkichi
authors: K
authors: Sugiyama
authors: Masahiko
authors: M
authors: Sai
authors: Kazuhisa
authors: K
authors: Maeda
authors: Tatsuhiko
authors: T
authors: Ito
mesh_terms: D001572:Benzofurans / Q000494:pharmacology*; D002460:Cell Line; D002477:Cells; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D029598:Passiflora; D010936:Plant Extracts / Q000494:pharmacology*; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012639:Seeds; D015595:Skin Aging; D013267:Stilbenes / Q000494:pharmacology*; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D001572:Benzofurans; D010936:Plant Extracts; D011837:Radiation-Protective Agents; D017382:Reactive Oxygen Species; D013267:Stilbenes; C546905:scirpusin B; C041525:3,3',4,5'-tetrahydroxystilbene; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1; D005978:Glutathione