title: Involvement of dopamine D2 receptors activation in ischemic post-conditioning-induced cardioprotection through promoting PKC-ε particulate translocation in isolated rat hearts.
abstract: Dopamine D2 receptors (DR2) are important regulators in many organs, including cardiac system. Protein kinase C (PKC) activation and translocation is associated with cardioprotection against ischemic post-conditioning (PC); however, the regulatory role of DR2 during this process has been unknown. This study hypothesized that the prevention of cardiomyocyte damage by DR2 activation is associated with PKC translocation to the cell membrane. In the present study, we found that the ischemia/reperfusion (I/R) increased the expressions of DR2 mRNA and protein, which were further enhanced by PC. Bromocriptine (DR2 agonist) up-regulated the PC-induced DR2 expressions, and Haloperidol (DR2 antagonist) reversed the increase of DR2 expressions by Bromocriptine. PC reduced I/R-induced cardiomyocytes damage, apoptosis and myocardial infarct size, and improved cardiac function. Compared with PC, Bromocriptine further enhanced the cardioprotective roles of PC, but Haloperidol canceled the protection effect of Bromocriptine. PC up-regulated PKC-ε translocation in the particulate fraction, which was further strengthened by Bromocriptine but canceled by Haloperidol. In the cytosolic fraction, the changes of the PKC-ε translocation were opposite to the particulate fraction. These findings suggest that DR2 activation provides cardioprotection via promoting PC-induced translocation of PKC-ε.
authors: Department of Pathophysiology, Harbin Medical University, Harbin, China.
authors: Jun
authors: J
authors: Gao
authors: Jin
authors: J
authors: Guo
authors: Hongxia
authors: H
authors: Li
authors: Shuzhi
authors: S
authors: Bai
authors: Hong
authors: H
authors: Li
authors: Bo
authors: B
authors: Wu
authors: Lina
authors: L
authors: Wang
authors: Yuhui
authors: Y
authors: Xi
authors: Ye
authors: Y
authors: Tian
authors: Guangdong
authors: G
authors: Yang
authors: Rui
authors: R
authors: Wang
authors: Lingyun
authors: L
authors: Wu
authors: Changqing
authors: C
authors: Xu
authors: Hongzhu
authors: H
authors: Li
mesh_terms: D000818:Animals; D017209:Apoptosis; D002374:Catalase / Q000378:metabolism; D003331:Coronary Vessels / Q000503:physiopathology; D003402:Creatine Kinase / Q000378:metabolism; D015870:Gene Expression; D066298:In Vitro Techniques; D057775:Ischemic Postconditioning; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D015428:Myocardial Reperfusion Injury / Q000201:enzymology* / Q000503:physiopathology / Q000628:therapy; D009206:Myocardium / Q000201:enzymology* / Q000473:pathology; D032383:Myocytes, Cardiac / Q000201:enzymology / Q000502:physiology; D051744:Protein Kinase C-epsilon / Q000378:metabolism*; D021381:Protein Transport; D051381:Rats; D017208:Rats, Wistar; D017448:Receptors, Dopamine D2 / Q000235:genetics / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D016277:Ventricular Function, Left; D017725:Ventricular Pressure
chemical_list: D017448:Receptors, Dopamine D2; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase; C497257:Prkce protein, rat; D051744:Protein Kinase C-epsilon; D003402:Creatine Kinase
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