title: Light-inducible molecular beacons for spatio-temporally highly defined activation.
abstract: We have developed a new molecular beacon design that requires an additional UV pulse for fluorescence activation. This improves the signal-to-noise ratio tremendously compared to previous approaches and allows for a precise control of the time point and location of RNA labelling.
authors: Goethe University Frankfurt, Institute for Organic Chemistry and Chemical Biology, Buchmann Institute for Molecular Life Sciences, Max-von-Laue-Str. 9, 60438 Frankfurt, Germany.
authors: Jennifer S
authors: JS
authors: Rinne
authors: Tim P
authors: TP
authors: Kaminski
authors: Ulrich
authors: U
authors: Kubitscheck
authors: Alexander
authors: A
authors: Heckel
mesh_terms: D000818:Animals; D002683:Chironomidae; D059045:Chromosomal Puffs / Q000235:genetics; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D018613:Microscopy, Confocal; D012333:RNA, Messenger / Q000032:analysis*; D012469:Salivary Glands / Q000166:cytology; D014466:Ultraviolet Rays
chemical_list: D005456:Fluorescent Dyes; D012333:RNA, Messenger