title: Expanding functions of intracellular resident mono-ADP-ribosylation in cell physiology.
abstract: Poly-ADP-ribosylation functions in diverse signaling pathways, such as Wnt signaling and DNA damage repair, where its role is relatively well characterized. Contrarily, mono-ADP-ribosylation by for example ARTD10/PARP10 is much less understood. Recent developments hint at the involvement of mono-ADP-ribosylation in transcriptional regulation, the unfolded protein response, DNA repair, insulin secretion and immunity. Additionally, macrodomain-containing hydrolases, MacroD1, MacroD2 and C6orf130/TARG1, have been identified that make mono-ADP-ribosylation reversible. Complicating further progress is the lack of tools such as mono-ADP-ribose-specific antibodies. The currently known functions of mono-ADP-ribosylation are summarized here, as well as the available tools such as mass spectrometry to study this modification in vitro and in cells.
authors: Institute of Biochemistry and Molecular Biology, RWTH Aachen University, Aachen, Germany.
authors: Karla L H
authors: KL
authors: Feijs
authors: Patricia
authors: P
authors: Verheugd
authors: Bernhard
authors: B
authors: Lüscher
mesh_terms: D000246:Adenosine Diphosphate Ribose / Q000378:metabolism*; D003593:Cytoplasm / Q000502:physiology*; D004249:DNA Damage / Q000502:physiology; D005786:Gene Expression Regulation; D006801:Humans; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D011499:Protein Processing, Post-Translational; D013312:Stress, Physiological / Q000502:physiology; D014158:Transcription, Genetic
chemical_list: D007328:Insulin; D000246:Adenosine Diphosphate Ribose
keywords: ADP-ribosylation; ARTD/PARP family; macrodomain-containing protein; mass spectrometry; stress response and inflammation