title: Differential effects of phosphatase inhibitors on the calcium homeostasis and migration of HaCaT keratinocytes.
abstract: Changes in intracellular calcium concentration ([Ca²⁺]i) as well as in the phosphorylation state of proteins have been implicated in keratinocyte wound healing revealed in scratch assays. Scratching confluent HaCaT monolayers decreased the number of cells displaying repetitive Ca²⁺ oscillations as well as the frequency of their Ca²⁺-transients in cells close to the wounded area and initiated migration of the cells into the wound bed. In contrast, calyculin-A (CLA) and okadaic acid (OA), known cell permeable inhibitors of protein phosphatase-1 and 2A, increased the level of resting [Ca²⁺]i and suppressed cell migration and wound healing of HaCaT cells. Furthermore, neither CLA nor OA influenced how scratching affected Ca²⁺ oscillations. It is assumed that changes in and alterations of the phosphorylation level of Ca²⁺-transport and contractile proteins upon phosphatase inhibition mediates cell migration and wound healing.
authors: Department of Physiology, University of Debrecen, Debrecen, Hungary.
authors: Olga
authors: O
authors: Ruzsnavszky
authors: Beatrix
authors: B
authors: Dienes
authors: Tamás
authors: T
authors: Oláh
authors: János
authors: J
authors: Vincze
authors: Tamás
authors: T
authors: Gáll
authors: Enikő
authors: E
authors: Balogh
authors: Gábor
authors: G
authors: Nagy
authors: Róbert
authors: R
authors: Bátori
authors: Beáta
authors: B
authors: Lontay
authors: Ferenc
authors: F
authors: Erdődi
authors: Laszlo
authors: L
authors: Csernoch
mesh_terms: D020013:Calcium Signaling; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006706:Homeostasis / Q000187:drug effects*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects* / Q000502:physiology*; D008387:Marine Toxins; D019319:Okadaic Acid / Q000494:pharmacology; D010080:Oxazoles / Q000494:pharmacology; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors*
chemical_list: D004791:Enzyme Inhibitors; D008387:Marine Toxins; D010080:Oxazoles; D019319:Okadaic Acid; C059041:calyculin A; D010749:Phosphoprotein Phosphatases
references: J Physiol. 2010 Nov 15;588(Pt 22):4453-74
references: 20876203
references: Cell Signal. 2008 Nov;20(11):2059-70
references: 18755268
references: Toxicol In Vitro. 2010 Feb;24(1):267-75
references: 19723577
references: Exp Eye Res. 2012 Jul;100:73-85
references: 22579493
references: Am J Physiol Cell Physiol. 2009 Jan;296(1):C88-96
references: 18987253
references: J Biochem Biophys Methods. 2004 Apr 30;59(1):89-101
references: 15134910
references: Inflammation. 1998 Dec;22(6):631-42
references: 9824776
references: Nat Rev Mol Cell Biol. 2003 Jul;4(7):517-29
references: 12838335
references: Exp Cell Res. 2010 Apr 1;316(6):980-91
references: 20132815
references: Biophys J. 2004 Mar;86(3):1436-53
references: 14990472
references: J Vasc Res. 1996 Sep-Oct;33(5):360-9
references: 8862141
references: J Theor Biol. 2010 Jun 21;264(4):1279-92
references: 20382167
references: Circ J. 2009 Jun;73(6):1133-40
references: 19377265
references: J Photochem Photobiol B. 2011 Oct 5;105(1):113-8
references: 21862341
references: Biochemistry. 1998 Dec 22;37(51):18102-9
references: 9922179
references: Mol Biol Cell. 2011 Mar 1;22(5):593-605
references: 21209320
references: PLoS One. 2009 Oct 05;4(10):e7330
references: 19802394
references: J Cell Biol. 1988 Mar;106(3):761-71
references: 2450098
references: Physiol Rev. 2003 Oct;83(4):1325-58
references: 14506307
references: J Invest Dermatol. 2011 May;131(5):1095-104
references: 21248768
references: J Cell Sci. 2003 Sep 15;116(Pt 18):3749-60
references: 12890758
references: J Biol Chem. 1985 Mar 25;260(6):3440-50
references: 3838314
references: Biochem Biophys Res Commun. 1989 Mar 31;159(3):871-7
references: 2539153
references: Nat Rev Mol Cell Biol. 2000 Oct;1(1):11-21
references: 11413485
references: Science. 2003 Dec 5;302(5651):1704-9
references: 14657486
references: Cell Signal. 2005 Oct;17(10):1265-75
references: 16038801
references: J Invest Dermatol. 1996 Sep;107(3):412-8
references: 8751979
references: J Cell Biochem. 2007 May 15;101(2):348-59
references: 17211850
references: Cell Calcium. 2008 Sep;44(3):310-23
references: 18291522
references: Biochem J. 1999 Aug 1;341 ( Pt 3):839-45
references: 10417351
references: J Biol Chem. 2003 Jun 20;278(25):22555-62
references: 12697752