title: Gold-nanopatterned single interleukin-6 sandwich immunoassay chips with zeptomolar detection capability based on evanescent field-enhanced fluorescence imaging.
abstract: We developed a gold-nanopatterned immunoassay chip for single-molecule detection of interleukin-6 based on evanescent field-enhanced fluorescence imaging. The detection limit (496 zM) means that only four molecules need to be present per spot for detection. The sensitivity of this chip is ~5.4 × 10(4)-fold greater than that of a commercial ELISA for IL-6.
authors: Department of Applied Chemistry, College of Applied Science, Kyung Hee University, Yongin-si, Gyeonggi-do 446-701, Republic of Korea.
authors: Seungah
authors: S
authors: Lee
authors: Seong Ho
authors: SH
authors: Kang
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods*; D003429:Cross Reactions; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D015850:Interleukin-6 / Q000032:analysis* / Q000097:blood / Q000276:immunology; D046208:Microchip Analytical Procedures / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D049329:Nanostructures / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D000911:Antibodies, Monoclonal; D015850:Interleukin-6; D006046:Gold