title: Simultaneous determination of 10 components in traditional Chinese medicine Dachaihu Granule by reversed-phase-high-performance liquid chromatographic-diode array detector.
abstract: BACKGROUND
  Dachaihu Granule, commonly used for treating cholecystitis, is derived from a famous traditional Chinese formula named Dachaihu Decoction. No analytical method has been reported for simultaneous determination of 10 bioactive compounds for quality control in Dachaihu Granule so far.
  
  
  OBJECTIVE
  To develop a high-performance liquid chromatographic (HPLC) method with diode array detector (DAD) for simultaneous determination of 10 bioactive compounds (paeoniflorin, aloe-emodin, rhein, emodin, chrysophanol, physcion, naringin, hesperidin, neohesperidin, and baicalin) in traditional Chinese medicine Dachaihu Granule.
  
  
  METHODS
  The samples were separated on a Kromasil C18 (250 × 4.6 mm,i.d. with 5.0 μm particle size)column with multi-wavelength detection method by a gradient elution using acetonitrile (A) and 0.2% acetic acid (B) as the mobile phase. The column temperature was maintained at 30°C and the detection wavelength was set at 230 nm for paeoniflorin, 254 nm for aloe-emodin, rhein, emodin, chrysophanol, and physcion, 280 nm for naringin, hesperidin, neohesperidin, and baicalin.
  
  
  RESULTS
  The developed method provided satisfactory precision and the accuracy of this method was in the range from 94.0% to 103.1%, all of the 10 compounds showed good linearity (r > 0.999) in a detected concentration range.
  
  
  CONCLUSIONS
  The validated method was successfully applied to the simultaneously of these active components in Dachaihu Granule from different production batches.
authors: College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, China.
authors: Yingfei
authors: Y
authors: Hu
authors: Tulin
authors: T
authors: Lu
authors: Chunqin
authors: C
authors: Mao
authors: Hao
authors: H
authors: Wu
authors: Xing
authors: X
authors: Zhang
authors: Jv
authors: J
authors: Wang
authors: Juanjuan
authors: J
authors: Gu
keywords: Dachaihu granule; high-performance liquid chromatographic-diode array detector; multi-components; multi-wavelength; quality control
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