title: Antiproliferative and cytotoxic properties of moxifloxacin on rat retinal ganglion cells.
abstract: Abstract Purpose: To evaluate the antiproliferative and cytotoxic properties of moxifloxacin on cultured rat retinal ganglion cells (RGC5).
  
  
  METHODS
  Rat retinal ganglion cells were exposed to various concentration of moxifloxacin (5-1500 μg/mL). For antiproliferative properties, the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) test was performed. Cellular cytotoxicity was assessed by using the Live/Dead viability/cytotoxicity assay and analyzed by fluorescence microscopy after 24 and 72 h of incubation, respectively.
  
  
  RESULTS
  Neither cytotoxic nor antiproliferative effect of moxifloxacin was observed below 50 μg/mL on RGC5 cells after 24 and 72 h of incubation. At higher concentrations of moxifloxacin (150 μg/mL, 500 μg/mL, and 1500 μg/mL (p < 0.001)) the number of viable cells and the proliferation rate of RGC5 were significantly reduced.
  
  
  CONCLUSIONS
  These results suggest a dose-dependent cytotoxic and antiproliferative effect of moxifloxacin on RGC5. Therefore, intracameral application of moxifloxacin should be used cautiously in patients with increased risk of retinal ganglion cells damage, particularly in glaucoma patients.
authors: University Eye Hospital, Centre for Ophthalmology, Eberhard-Karls University, Tuebingen, Germany.
authors: Bianka
authors: B
authors: Sobolewska
authors: Johanna
authors: J
authors: Hofmann
authors: Martin S
authors: MS
authors: Spitzer
authors: Karl U
authors: KU
authors: Bartz-Schmidt
authors: Peter
authors: P
authors: Szurman
authors: Efdal
authors: E
authors: Yoeruek
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D001372:Aza Compounds / Q000633:toxicity*; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D009877:Endophthalmitis / Q000188:drug therapy*; D024841:Fluoroquinolones; D000077266:Moxifloxacin; D011804:Quinolines / Q000633:toxicity*; D051381:Rats; D012165:Retinal Ganglion Cells / Q000166:cytology* / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D001372:Aza Compounds; D024841:Fluoroquinolones; D011804:Quinolines; D000077266:Moxifloxacin