title: Novel real-time polymerase chain reactions for serogroup specific gene detection of Neisseria meningitidis serogroups B, C, W-135 and Y.
abstract: Novel real-time TaqMan primers and probes were developed to detect the serogroup specific genes of Neisseria meningitidis serogroups B, C, W-135 and Y. These assays were rapid, sensitive, and specific when extensively validated.
authors: Public Health Microbiology, Communicable Disease, Forensic and Scientific Services, Queensland Government Department of Health, Australia. Electronic address: christine_doyle@health.qld.gov.au.
authors: Christine J
authors: CJ
authors: Doyle
authors: Public Health Microbiology, Communicable Disease, Forensic and Scientific Services, Queensland Government Department of Health, Australia.
authors: Amy V
authors: AV
authors: Jennison
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D006801:Humans; D008585:Meningitis, Meningococcal / Q000382:microbiology; D009345:Neisseria meningitidis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D012703:Serotyping / Q000379:methods*; D013045:Species Specificity
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers
keywords: IMD; LLD; Meningococcal; Neisseria meningitidis; PCR; Real-time PCR; Serogroup; TaqMan; invasive meningococcal disease; lower limit of detection; polymerase chain reaction