title: Environmental factors influencing the prevalence of a Clostridium botulinum type C/D mosaic strain in nonpermanent Mediterranean wetlands.
abstract: Between 1978 and 2008, 13 avian botulism outbreaks were recorded in the wetlands of Mancha Húmeda (central Spain). These outbreaks caused the deaths of around 20,000 birds from over 50 species, including globally endangered white-headed ducks (Oxyura leucoceophala). Here, a significant association was found between the number of dead birds recorded in each botulism outbreak and the mean temperature in July (always >26°C). The presence of Clostridium botulinum type C/D in wetland sediments was detected by real-time PCR (quantitative PCR [qPCR]) in 5.8% of 207 samples collected between 2005 and 2008. Low concentrations of Cl(-) and high organic matter content in sediments were significantly associated with the presence of C. botulinum. Seventy-five digestive tracts of birds found dead during botulism outbreaks were analyzed; C. botulinum was present in 38.7% of them. The prevalence of C. botulinum was 18.2% (n = 22 pools) in aquatic invertebrates (Chironomidae and Corixidae families) and 33.3% (n = 18 pools) in necrophagous invertebrates (Sarcophagidae and Calliphoridae families), including two pools of adult necrophagous flies collected around bird carcasses. The presence of the bacteria in the adult fly form opens up new perspectives in the epidemiology of avian botulism, since these flies may be transporting C. botulinum from one carcass to another.
authors: Instituto de Investigación en Recursos Cinegéticos (IREC), CSIC-UCLM-JCCM, Ciudad Real, Spain. mariadolors.vidal@uclm.es
authors: Dolors
authors: D
authors: Vidal
authors: Ibone
authors: I
authors: Anza
authors: Mark A
authors: MA
authors: Taggart
authors: Elisa
authors: E
authors: Pérez-Ramírez
authors: Elena
authors: E
authors: Crespo
authors: Ursula
authors: U
authors: Hofle
authors: Rafael
authors: R
authors: Mateo
mesh_terms: D000818:Animals; D001431:Bacteriological Techniques / Q000662:veterinary; D001715:Bird Diseases / Q000453:epidemiology / Q000382:microbiology* / Q000635:transmission; D001717:Birds; D001906:Botulism / Q000453:epidemiology / Q000382:microbiology / Q000635:transmission / Q000662:veterinary*; D047968:Clostridium botulinum type C / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D047969:Clostridium botulinum type D / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004196:Disease Outbreaks / Q000662:veterinary; D004372:Ducks; D056727:Endangered Species; D019015:Geologic Sediments / Q000737:chemistry / Q000382:microbiology*; D007313:Insecta / Q000382:microbiology; D015995:Prevalence; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D012621:Seasons; D013030:Spain / Q000453:epidemiology; D014887:Weather; D053833:Wetlands
references: Appl Environ Microbiol. 2007 Apr;73(7):2118-27
references: 17277206
references: Vet Rec. 1991 Feb 23;128(8):187-8
references: 2031295
references: J Wildl Dis. 2010 Apr;46(2):507-13
references: 20688643
references: J Vet Diagn Invest. 2011 Sep;23(5):942-6
references: 21908351
references: J Wildl Dis. 1984 Oct;20(4):267-71
references: 6530712
references: Math Biosci. 2008 Nov;216(1):17-29
references: 18761358
references: J Am Vet Med Assoc. 1980 Nov 1;177(9):827-8
references: 7451318
references: J Wildl Dis. 1987 Jan;23(1):67-76
references: 3546742
references: Sci Total Environ. 2012 Jul 15;430:174-83
references: 22647241
references: J Wildl Dis. 1988 Apr;24(2):240-5
references: 3373631
references: Avian Dis. 2005 Sep;49(3):376-81
references: 16252491
references: Appl Environ Microbiol. 2002 Jan;68(1):124-37
references: 11772618
references: Avian Dis. 1982 Jan-Mar;26(1):89-96
references: 7046722
references: Adv Microb Physiol. 2009;55:183-265, 320
references: 19573697
references: J Hyg (Lond). 1982 Dec;89(3):507-11
references: 6759578
references: J Wildl Dis. 2010 Apr;46(2):499-506
references: 20688642
references: Rev Sci Tech. 1991 Sep;10(3):749-64
references: 1782427
references: Vet Rec. 1977 Feb 5;100(6):106-9
references: 320751
references: J Wildl Dis. 1997 Apr;33(2):181-6
references: 9131546
references: J Wildl Dis. 1993 Oct;29(4):533-9
references: 8258850
references: Vet Microbiol. 2010 Nov 20;146(1-2):118-23
references: 20537470
references: J Wildl Dis. 1991 Jan;27(1):81-5
references: 2023331
references: J Wildl Dis. 1983 Oct;19(4):302-7
references: 6358539
references: J Wildl Dis. 1984 Apr;20(2):86-9
references: 6737616
references: J Wildl Dis. 1983 Apr;19(2):90-4
references: 6887447
references: J Wildl Dis. 2010 Jul;46(3):951-5
references: 20688704
references: Acta Microbiol Immunol Hung. 2008 Dec;55(4):455-64
references: 19130753
references: Avian Pathol. 2010 Dec;39(6):511-8
references: 21154062
references: J Wildl Dis. 1993 Jan;29(1):8-14
references: 8445793
references: ALTEX. 2005;22(3):185-95
references: 16186994
references: Appl Environ Microbiol. 2012 May;78(9):3120-7
references: 22344654
references: Appl Microbiol. 1971 Dec;22(6):1017-24
references: 4944800
references: J Wildl Dis. 1976 Jan;12(1):116-26
references: 1255907
references: J Wildl Dis. 2010 Jul;46(3):864-77
references: 20688692