title: New advances in molecular recognition based on biomolecular scaffolds.
abstract: In this review, we summarize recent advances in the development of molecular recognition components based on the biomolecular scaffolds of proteins and nucleic acids for specific recognition of miscellaneous targets. In addition to the widely adopted recombinant antibody fragments, designed ankyrin repeat proteins and modular peptide repeats of transcription-activator-like effectors for base-specific recognition of DNA sequence are also briefly introduced. For the nucleic acid based molecular recognition systems, aptamers, including slow off-rate modified aptamers, DNAzymes, and synthetic DNA-like oligomers for versatile biorecognition are described. Finally, we discuss the remaining challenges and future research directions in the field.
authors: Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, China. mpzhao@pku.edu.cn
authors: Meiping
authors: M
authors: Zhao
authors: Tongbo
authors: T
authors: Wu
authors: Xianjin
authors: X
authors: Xiao
authors: Yang
authors: Y
authors: Liu
authors: Xin
authors: X
authors: Su
mesh_terms: D000818:Animals; D006801:Humans; D009696:Nucleic Acids / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010455:Peptides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015202:Protein Engineering; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D009696:Nucleic Acids; D010455:Peptides; D011506:Proteins; D011994:Recombinant Proteins