title: Anti-senescence efficacy of radio-electric asymmetric conveyer technology.
abstract: Recent evidence suggests that ageing-related diseases could result in an accelerated loss of self-renewal capability of adult stem cells, normally involved in replacing damaged cellular elements. In previous works, we highlighted that a specific treatment, named tissue optimization-regenerative (TO-RGN), of radio-electric asymmetric conveyer (REAC) technology, influenced gene expression profiles controlling stem cell differentiation and pluripotency of human skin-derived fibroblasts in vitro. The purpose of the present work was to verify whether TO-RGN may also be effective in counteracting the expression of the senescence marker beta-galactosidase and of senescence-associated gene expression patterning, engaged during prolonged culture of human adipose-derived stem cells (hADSCs). Following TO-RGN exposure, we observed a significant downregulation in beta-galactosidase staining and in the expression of the senescence mediator genes p16INK4, ARF, p53, and p21(CIP1). Moreover, differently formed untreated cells, TO-RGN-exposed hADSCs maintained their typical fibroblast-like morphology and exhibited a multilineage potential even at late passages, as shown by the remarkable preservation of commitment to osteogenic, adipogenic, chondrogenic, and vasculogenic fates, both at morphologic and gene expression levels. In conclusion, our study highlights a positive effect of TO-RGN in counteracting degenerative senescence processes in vitro.
authors: Department of Biomedical Sciences, University of Sassari, Viale San Pietro, 43/B, 07100, Sassari, Italy.
authors: Margherita
authors: M
authors: Maioli
authors: Salvatore
authors: S
authors: Rinaldi
authors: Sara
authors: S
authors: Santaniello
authors: Alessandro
authors: A
authors: Castagna
authors: Gianfranco
authors: G
authors: Pigliaru
authors: Alessandro
authors: A
authors: Delitala
authors: Matteo
authors: M
authors: Lotti Margotti
authors: Luigi
authors: L
authors: Bagella
authors: Vania
authors: V
authors: Fontani
authors: Carlo
authors: C
authors: Ventura
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D002454:Cell Differentiation / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D016922:Cellular Senescence / Q000235:genetics / Q000528:radiation effects*; D004558:Electric Stimulation; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression; D006801:Humans; D008875:Middle Aged; D013194:Staining and Labeling; D013234:Stem Cells / Q000166:cytology*; D015854:Up-Regulation; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D001616:beta-Galactosidase
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