title: Lentivirus restriction by diverse primate APOBEC3A proteins.
abstract: Rhesus macaque APOBEC3A (rhA3A) is capable of restricting both simian-human immunodeficiency virus (SHIVΔvif) and human immunodeficiency virus (HIV-1Δvif) to a greater extent than hA3A. We constructed chimeric A3A proteins to define the domains required for differential lentivirus restriction. Substitution of amino acids 25-33 from rhA3A into hA3A was sufficient to restrict HIVΔvif to levels similar to rhA3A restriction of SHIVΔvif. We tested if differential lentivirus restriction is conserved between A3A from Old World monkey and hominid lineages. A3A from African green monkey restricted SHIVΔvif but not HIV-1Δvif and colobus monkey A3A restricted both wild type and SHIVΔvif and HIV-1Δvif. In contrast, the gibbon ape A3A restricted neither SHIVΔvif nor HIV-1Δvif. Restriction of SHIVΔvif and HIV-1Δvif by New World monkey A3A proteins was not conserved as the A3A from the squirrel monkey but not the northern owl monkey restricted SHIVΔvif. Finally, the colobus A3A protein appears to restrict by a novel post-entry mechanism.
authors: Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Center, 3901 Rainbow Blvd. Kansas City, KS 66160, United States.
authors: Kimberly
authors: K
authors: Schmitt
authors: Kejun
authors: K
authors: Guo
authors: Miki
authors: M
authors: Katuwal
authors: Darayu
authors: D
authors: Wilson
authors: Courtney
authors: C
authors: Prochnow
authors: Ronda
authors: R
authors: Bransteitter
authors: Xiaojiang S
authors: XS
authors: Chen
authors: Mario L
authors: ML
authors: Santiago
authors: Edward B
authors: EB
authors: Stephens
mesh_terms: D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D003564:Cytidine Deaminase / Q000235:genetics / Q000378:metabolism*; D015658:HIV Infections / Q000821:virology; D015497:HIV-1 / Q000472:pathogenicity* / Q000502:physiology; D006801:Humans; D016086:Lentivirus / Q000472:pathogenicity / Q000502:physiology; D008253:Macaca mulatta / Q000821:virology; D011323:Primates / Q000821:virology*; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D016097:Simian Acquired Immunodeficiency Syndrome / Q000821:virology; D015302:Simian Immunodeficiency Virus / Q000378:metabolism / Q000472:pathogenicity* / Q000502:physiology; D014779:Virus Replication
chemical_list: D011506:Proteins; C121399:APOBEC3A protein, human; D003564:Cytidine Deaminase
references: Nature. 2002 Aug 8;418(6898):646-50
references: 12167863
references: J Biol Chem. 2004 Feb 27;279(9):7792-8
references: 14672928
references: Antimicrob Agents Chemother. 2002 Jun;46(6):1896-905
references: 12019106
references: Nature. 2005 Mar 17;434(7031):376-80
references: 15772660
references: Philos Trans R Soc Lond B Biol Sci. 2009 Mar 12;364(1517):689-703
references: 19008196
references: Clin Microbiol Rev. 2001 Jan;14(1):165-76
references: 11148008
references: Science. 2003 Nov 7;302(5647):1056-60
references: 14564014
references: Proc Natl Acad Sci U S A. 2003 Apr 29;100(9):5280-5
references: 12682288
references: Expert Rev Anti Infect Ther. 2009 Jun;7(5):569-80
references: 19485797
references: J Biol Chem. 2006 Aug 4;281(31):22161-22172
references: 16735504
references: Nat Struct Mol Biol. 2004 May;11(5):435-42
references: 15098018
references: Science. 2003 Jun 13;300(5626):1713
references: 12805540
references: Am J Phys Anthropol. 2007 Mar;132(3):344-54
references: 17133436
references: J Virol. 2010 Dec;84(24):12599-608
references: 20943965
references: Virology. 2005 Sep 1;339(2):281-8
references: 15993456
references: J Virol. 2011 Feb;85(4):1765-76
references: 21123384
references: Virology. 2009 Jan 20;383(2):362-72
references: 19027134
references: J Virol. 2007 Dec;81(24):13932-7
references: 17942564
references: Cell Host Microbe. 2008 Sep 11;4(3):249-59
references: 18779051
references: Nat Med. 2003 Nov;9(11):1404-7
references: 14528300
references: Virology. 2003 May 10;309(2):248-57
references: 12758172
references: Expert Opin Biol Ther. 2009 Nov;9(11):1427-36
references: 19743892
references: Virology. 2010 Sep 1;404(2):187-203
references: 20627348
references: Proc Natl Acad Sci U S A. 2006 Jun 6;103(23):8780-5
references: 16728505
references: J Virol. 2010 Jan;84(1):88-95
references: 19828612
references: J Virol. 2001 Jan;75(2):857-66
references: 11134299
references: J Virol. 2005 Jul;79(14):8724-31
references: 15994766
references: J Biol Chem. 1995 Jun 16;270(24):14768-75
references: 7782343
references: Virology. 1999 Aug 1;260(2):295-307
references: 10417264
references: EMBO Rep. 2011 May;12(5):444-50
references: 21460793
references: Hum Mutat. 2007 Jun;28(6):527-39
references: 17309057
references: EMBO J. 2004 Jun 16;23(12):2451-8
references: 15152192
references: Nature. 2006 Nov 9;444(7116):164
references: 17093443
references: Mol Biol Evol. 2003 Oct;20(10):1620-5
references: 12832653
references: J Virol. 2006 Nov;80(21):10522-33
references: 16920826
references: J Virol. 2011 Apr;85(7):3142-52
references: 21270145
references: Nature. 2011 Dec 21;481(7381):371-5
references: 22190037
references: Retrovirology. 2007 Aug 29;4:61
references: 17727729
references: J Virol. 2009 Jan;83(1):295-303
references: 18945781
references: Curr Top Microbiol Immunol. 2003;277:181-96
references: 12908773
references: Virology. 2008 Jul 20;377(1):71-9
references: 18495196
references: PLoS Pathog. 2007 Oct 26;3(10):1477-85
references: 17967058
references: J Virol. 2012 Jun;86(11):6097-108
references: 22457529
references: Science. 2002 Jan 18;295(5554):465
references: 11799233
references: Nat Struct Mol Biol. 2006 May;13(5):392-9
references: 16622407
references: Curr Biol. 2004 Aug 10;14(15):1392-6
references: 15296758
references: J Biol Chem. 2010 Sep 3;285(36):27753-66
references: 20615867
references: PLoS Pathog. 2009 May;5(5):e1000439
references: 19461882
references: J Virol. 2010 Aug;84(16):7961-9
references: 20519396
references: J Virol. 2004 Feb;78(4):2072-81
references: 14747572
references: J Biol Chem. 2008 Feb 1;283(5):2518-25
references: 18057006
references: Nat Struct Mol Biol. 2010 Feb;17(2):222-9
references: 20062055
references: PLoS Pathog. 2013 Jan;9(1):e1003135
references: 23359341
references: Nucleic Acids Res. 2005 Apr 04;33(6):1913-23
references: 15809227
references: J Virol. 2003 Jul;77(14):7830-42
references: 12829823
references: J Virol. 1995 Sep;69(9):5261-8
references: 7636968
references: Proc Natl Acad Sci U S A. 2005 May 31;102(22):7982-7
references: 15911774
references: Curr Gene Ther. 2007 Aug;7(4):261-71
references: 17969559
references: Nature. 2008 Nov 6;456(7218):121-4
references: 18849968
references: J Virol. 2011 Nov;85(21):11220-34
references: 21835787
references: Nucleic Acids Res. 2006 Jan 10;34(1):89-95
references: 16407327
references: J Virol. 2004 Mar;78(6):2780-9
references: 14990698
references: Curr Biol. 2006 Mar 7;16(5):480-5
references: 16527742
references: Nature. 2011 Dec 21;481(7381):376-9
references: 22190036
references: J Virol. 2008 Aug;82(15):7741-4
references: 18508895
references: Virology. 2012 Mar 15;424(2):138-46
references: 22265237
references: Nat Struct Mol Biol. 2012 Oct;19(10):1005-10
references: 23001005
references: Genomics. 2002 Mar;79(3):285-96
references: 11863358
references: Virology. 2011 Oct 10;419(1):24-42
references: 21868050
references: Virology. 2001 Sep 1;287(2):310-20
references: 11531409
references: Nat Med. 2003 Nov;9(11):1398-403
references: 14528301
references: J Virol. 2000 Sep;74(18):8358-67
references: 10954535