title: CYCLIN H;1 regulates drought stress responses and blue light-induced stomatal opening by inhibiting reactive oxygen species accumulation in Arabidopsis.
abstract: Arabidopsis (Arabidopsis thaliana) CYCLIN-DEPENDENT KINASE Ds (CDKDs) phosphorylate the C-terminal domain of the largest subunit of RNA polymerase II. Arabidopsis CYCLIN H;1 (CYCH;1) interacts with and activates CDKDs; however, the physiological function of CYCH;1 has not been determined. Here, we report that CYCH;1, which is localized to the nucleus, positively regulates blue light-induced stomatal opening. Reduced-function cych;1 RNA interference (cych;1 RNAi) plants exhibited a drought tolerance phenotype. CYCH;1 is predominantly expressed in guard cells, and its expression was substantially down-regulated by dehydration. Transpiration of intact leaves was reduced in cych;1 RNAi plants compared with the wild-type control in light but not in darkness. CYCH;1 down-regulation impaired blue light-induced stomatal opening but did not affect guard cell development or abscisic acid-mediated stomatal closure. Microarray and real-time polymerase chain reaction analyses indicated that CYCH;1 did not regulate the expression of abscisic acid-responsive genes or light-induced stomatal opening signaling determinants, such as MYB60, MYB61, Hypersensitive to red and blue1, and Protein phosphatase7. CYCH;1 down-regulation induced the expression of redox homeostasis genes, such as LIPOXYGENASE3 (LOX3), LOX4, ARABIDOPSIS GLUTATHIONE PEROXIDASE 7 (ATGPX7), EARLY LIGHT-INDUCIBLE PROTEIN1 (ELIP1), and ELIP2, and increased hydrogen peroxide production in guard cells. Furthermore, loss-of-function mutations in CDKD;2 or CDKD;3 did not affect responsiveness to drought stress, suggesting that CYCH;1 regulates the drought stress response in a CDKD-independent manner. We propose that CYCH;1 regulates blue light-mediated stomatal opening by controlling reactive oxygen species homeostasis.
authors: Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
authors: Xiao Feng
authors: XF
authors: Zhou
authors: Yin Hua
authors: YH
authors: Jin
authors: Chan Yul
authors: CY
authors: Yoo
authors: Xiao-Li
authors: XL
authors: Lin
authors: Woe-Yeon
authors: WY
authors: Kim
authors: Dae-Jin
authors: DJ
authors: Yun
authors: Ray A
authors: RA
authors: Bressan
authors: Paul M
authors: PM
authors: Hasegawa
authors: Jing Bo
authors: JB
authors: Jin
mesh_terms: D017360:Arabidopsis / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D056748:Cyclin H / Q000235:genetics / Q000378:metabolism*; D018844:Cyclin-Dependent Kinases / Q000235:genetics / Q000378:metabolism; D055864:Droughts; D018506:Gene Expression Regulation, Plant; D008027:Light; D009154:Mutation; D054046:Plant Stomata / Q000502:physiology*; D018526:Plant Transpiration; D030821:Plants, Genetically Modified; D034622:RNA Interference; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: D029681:Arabidopsis Proteins; D056748:Cyclin H; C481743:ELIP2 protein, Arabidopsis; D017382:Reactive Oxygen Species; C585933:cyclin H, Arabidopsis; C514673:CDKD;2 protein, Arabidopsis; D018844:Cyclin-Dependent Kinases
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