title: Enterobacter-activated mosquito immune responses to Plasmodium involve activation of SRPN6 in Anopheles stephensi.
abstract: Successful development of Plasmodium in the mosquito is essential for the transmission of malaria. A major bottleneck in parasite numbers occurs during midgut invasion, partly as a consequence of the complex interactions between the endogenous microbiota and the mosquito immune response. We previously identified SRPN6 as an immune component which restricts Plasmodium berghei development in the mosquito. Here we demonstrate that SRPN6 is differentially activated by bacteria in Anopheles stephensi, but only when bacteria exposure occurs on the lumenal surface of the midgut epithelium. Our data indicate that AsSRPN6 is strongly induced following exposure to Enterobacter cloacae, a common component of the mosquito midgut microbiota. We conclude that AsSRPN6 is a vital component of the E. cloacae-mediated immune response that restricts Plasmodium development in the mosquito An. stephensi.
authors: Department of Molecular Microbiology and Immunology, Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, United States of America.
authors: Abraham G
authors: AG
authors: Eappen
authors: Ryan C
authors: RC
authors: Smith
authors: Marcelo
authors: M
authors: Jacobs-Lorena
mesh_terms: D000818:Animals; D000852:Anopheles / Q000235:genetics / Q000276:immunology* / Q000382:microbiology / Q000469:parasitology; D016972:Enterobacter cloacae / Q000502:physiology*; D005786:Gene Expression Regulation / Q000276:immunology; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins; D054884:Host-Pathogen Interactions; D019476:Insect Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000276:immunology*; D007413:Intestinal Mucosa / Q000276:immunology / Q000382:microbiology / Q000469:parasitology; D010962:Plasmodium berghei / Q000502:physiology*; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D015843:Serpins / Q000235:genetics / Q000276:immunology*; D015398:Signal Transduction; D013559:Symbiosis / Q000276:immunology*
chemical_list: D019476:Insect Proteins; D034741:RNA, Small Interfering; D015843:Serpins; D049452:Green Fluorescent Proteins
references: Science. 2010 Sep 10;329(5997):1353-5
references: 20829487
references: PLoS Pathog. 2011 May;7(5):e1002043
references: 21625582
references: Cell. 2009 Dec 24;139(7):1268-78
references: 20064373
references: PLoS One. 2011;6(9):e24767
references: 21957459
references: EMBO Rep. 2002 Sep;3(9):852-6
references: 12189180
references: PLoS Pathog. 2012;8(10):e1002965
references: 23093936
references: Immunol Rev. 2004 Apr;198:127-48
references: 15199960
references: Parasitol Today. 2000 May;16(5):196-201
references: 10782078
references: Science. 2009 Apr 10;324(5924):258-61
references: 19264986
references: Insect Mol Biol. 2008 Feb;17(1):39-51
references: 18237283
references: Dev Comp Immunol. 2009 Jul;33(7):830-7
references: 19428484
references: J Biol Chem. 2004 Feb 13;279(7):5581-7
references: 14627711
references: Proc Natl Acad Sci U S A. 2012 Jul 31;109(31):12734-9
references: 22802646
references: PLoS Pathog. 2011 Sep;7(9):e1002259
references: 21980286
references: PLoS Pathog. 2007 Dec 28;3(12):e195
references: 18166078
references: PLoS Pathog. 2009 Aug;5(8):e1000542
references: 19662170
references: Exp Parasitol. 1993 Sep;77(2):195-9
references: 8375488
references: Insect Biochem Mol Biol. 1997 Dec;27(12):1063-72
references: 9569647
references: Science. 2010 Mar 26;327(5973):1644-8
references: 20223948
references: Proc Natl Acad Sci U S A. 1997 Oct 14;94(21):11508-13
references: 9326640
references: Cell. 2004 Mar 5;116(5):661-70
references: 15006349
references: Arch Insect Biochem Physiol. 2001 Jun;47(2):119-25
references: 11376458
references: EMBO J. 1997 Oct 15;16(20):6114-9
references: 9321391
references: Cell Host Microbe. 2008 Jun 12;3(6):364-74
references: 18541213
references: Proc Natl Acad Sci U S A. 2001 Oct 23;98(22):12630-5
references: 11606751
references: PLoS One. 2012;7(11):e48689
references: 23152794
references: Immunity. 2010 Jan 29;32(1):41-53
references: 20152169
references: Exp Parasitol. 1999 Jan;91(1):59-69
references: 9920043
references: Am J Trop Med Hyg. 1996 Feb;54(2):214-8
references: 8619451
references: PLoS Pathog. 2009 May;5(5):e1000423
references: 19424427
references: Proc Natl Acad Sci U S A. 2005 Nov 8;102(45):16327-32
references: 16260729
references: Science. 2012 Feb 17;335(6070):856-9
references: 22282475
references: Parasite Immunol. 2006 Apr;28(4):121-30
references: 16542314
references: Infect Immun. 2011 Jan;79(1):321-9
references: 20956566
references: J Biol Chem. 2004 Feb 13;279(7):5573-80
references: 14627712
references: Cell Mol Life Sci. 2011 Jun;68(11):1929-39
references: 20953892
references: Science. 2011 May 13;332(6031):855-8
references: 21566196
references: Cell Host Microbe. 2009 Mar 19;5(3):273-84
references: 19286136
references: Cell Microbiol. 2008 Apr;10(4):891-8
references: 18005239
references: Insect Mol Biol. 1996 Nov;5(4):261-8
references: 8933177
references: Parasitology. 1985 Oct;91 ( Pt 2):227-44
references: 3906519
references: Dev Comp Immunol. 2010 Apr;34(4):387-95
references: 20026176
references: PLoS Pathog. 2006 Jun;2(6):e52
references: 16789837