title: GOLLUM [FeFe]-hydrogenase-like proteins are essential for plant development in normoxic conditions and modulate energy metabolism.
abstract: [FeFe]-hydrogenase-like genes encode [Fe4 S4]-containing proteins that are ubiquitous in eukaryotic cells. In humans, iron-only hydrogenase-like protein 1 (IOP1) represses hypoxia inducible factor-1α subunit (HIF1-α) at normal atmospheric partial O2 pressure (normoxia, 21 kPa O2). In yeasts, the nar1 mutant cannot grow at 21 kPa O2, but can develop at a lower O2 pressure (2 kPa O2). We show here that plant [FeFe]-hydrogenase-like GOLLUM genes are essential for plant development and cell cycle progression. The mutant phenotypes of these plants are seen in normoxic conditions, but not under conditions of mild hypoxia (5 kPa O2). Transcriptomic and metabolomic experiments showed that the mutation enhances the expression of some hypoxia-induced genes under normal atmospheric O2 conditions and changes the cellular content of metabolites related to energy metabolism. In conclusion, [FeFe]-hydrogenase-like proteins play a central role in eukaryotes including the adaptation of plants to the ambient O2 partial pressure.
authors: CNRS, Institut des Sciences du Végétal, Av. de la Terrasse, F-91198, Gif sur Yvette, France.
authors: Samuel
authors: S
authors: Mondy
authors: Aurore
authors: A
authors: Lenglet
authors: Viviane
authors: V
authors: Cosson
authors: Sandra
authors: S
authors: Pelletier
authors: Stéphanie
authors: S
authors: Pateyron
authors: Françoise
authors: F
authors: Gilard
authors: Marije
authors: M
authors: Scholte
authors: Lysiane
authors: L
authors: Brocard
authors: Jean-Malo
authors: JM
authors: Couzigou
authors: Guillaume
authors: G
authors: Tcherkez
authors: Michel
authors: M
authors: Péan
authors: Pascal
authors: P
authors: Ratet
mesh_terms: D000222:Adaptation, Physiological; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D050260:Carbohydrate Metabolism; D002453:Cell Cycle; D015536:Down-Regulation; D004734:Energy Metabolism; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D006864:Hydrogenase / Q000235:genetics* / Q000378:metabolism; D007506:Iron-Sulfur Proteins / Q000235:genetics* / Q000378:metabolism; D046913:Medicago truncatula / Q000201:enzymology* / Q000235:genetics / Q000502:physiology; D055432:Metabolomics; D009154:Mutation; D010100:Oxygen / Q000378:metabolism*; D010641:Phenotype; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D036226:Seedlings / Q000201:enzymology / Q000235:genetics / Q000502:physiology; D013312:Stress, Physiological; D059467:Transcriptome; D015854:Up-Regulation
chemical_list: D007506:Iron-Sulfur Proteins; D010940:Plant Proteins; C118830:iron hydrogenase; D006864:Hydrogenase; D010100:Oxygen
keywords: Arabidopsis; HIF-1α; IOP; Medicago; NAR1; Warburg effect; oxygen