title: Inhibitory effects of Saururi chinensis extracts on melanin biosynthesis in B16F10 melanoma cells.
abstract: Saururus chinensis has been used in folk medicine in Korea for the treatment of edema, jaundice, gonorrhea, and several inflammatory diseases. Saururi chinensis extracts (SCE) have demonstrated anti-inflammatory and anti-oxidant activities, as well as anti-asthmatic, antihypertensive, anti-angiogenic, and therapeutic activities for atopic dermatitis. However, the inhibitory activity of SCE on the melanogenesis signaling pathway is not completely understood. This study examined the effects of SCE on the melanogenesis signaling pathway activated by α-melanocyte-stimulating hormone (α-MSH). We found that SCE inhibited melanin production in a dose-dependent manner without causing cytotoxicity in B16F10 cells. Interestingly, SCE decreased α-MSH-induced tyrosinase activity in B16F10 cells but did not inhibit tyrosinase activity under cell-free conditions. The results of this study indicate that SCE may reduce pigmentation by way of an indirect, nonenzymatic mechanism. We also found that SCE decreased α-MSH-induced microphthalmia-associated transcription factor (MITF) and tyrosinase expression and induced the activation of extracellular signal-regulated kinase (ERK). These results suggest that the depigmenting effect of SCE may result from downregulation of MITF and tyrosinase expression due to increased ERK activity. Thus, our results provide evidence that SCE might be useful as a potential skin-whitening agent.
authors: Nutrex Technology Co, Ltd, Songpa-Gu, Seoul 138–962, Korea.
authors: Do Hyun
authors: DH
authors: Lee
authors: Dong Ha
authors: DH
authors: Kim
authors: In Young
authors: IY
authors: Oh
authors: So Young
authors: SY
authors: Kim
authors: Yun Young
authors: YY
authors: Lim
authors: Hyeong Mi
authors: HM
authors: Kim
authors: Young Heui
authors: YH
authors: Kim
authors: Yoo Mi
authors: YM
authors: Choi
authors: Sung Eun
authors: SE
authors: Kim
authors: Beom Joon
authors: BJ
authors: Kim
authors: Myeung Nam
authors: MN
authors: Kim
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008543:Melanins / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D008546:Melanoma, Experimental / Q000378:metabolism; D051379:Mice; D051739:Microphthalmia-Associated Transcription Factor / Q000378:metabolism; D014442:Monophenol Monooxygenase / Q000378:metabolism; D010766:Phosphorylation; D010936:Plant Extracts / Q000494:pharmacology*; D032134:Saururaceae; D062327:Skin Lightening Preparations / Q000494:pharmacology*
chemical_list: D008543:Melanins; D051739:Microphthalmia-Associated Transcription Factor; C497235:Mitf protein, mouse; D010936:Plant Extracts; D062327:Skin Lightening Preparations; D014442:Monophenol Monooxygenase; D048049:Extracellular Signal-Regulated MAP Kinases