title: Parallel on-chip analysis of single vesicle neurotransmitter release.
abstract: Real-time investigations of neurotransmitter release provide a direct insight on the mechanisms involved in synaptic communication. Carbon fiber microelectrodes are state-of-the-art tools for electrochemical measurements of single vesicle neurotransmitter release. Yet, they lack high-throughput capabilities that are required for collecting robust statistically significant data across multiple samples. Here, we present a chip-based recording system enabling parallel in vitro measurements of individual neurotransmitter release events from cells, cultured directly on planar multielectrode arrays. The applicability of this cell-based platform to pharmacological screening is demonstrated by resolving minute concentration-dependent effects of the dopamine reuptake inhibitor nomifensine on recorded single-vesicle release events from PC12 cells. The experimental results, showing an increased half-time of the recorded events, are complemented by an analytical model for the verification of drug action.
authors: Institute of Bioelectronics (PGI-8/ICS-8) and JARA--Fundamentals of Future Information Technology, Forschungszentrum Jülich, 52425 Jülich, Germany.
authors: Alexey
authors: A
authors: Yakushenko
authors: Enno
authors: E
authors: Kätelhön
authors: Bernhard
authors: B
authors: Wolfrum
mesh_terms: D000818:Animals; D002244:Carbon / Q000737:chemistry*; D000077482:Carbon Fiber; D003198:Computer Simulation; D004298:Dopamine / Q000032:analysis* / Q000378:metabolism; D018765:Dopamine Uptake Inhibitors / Q000494:pharmacology; D005089:Exocytosis / Q000187:drug effects; D056656:Lab-On-A-Chip Devices; D008839:Microelectrodes; D009627:Nomifensine / Q000494:pharmacology; D016716:PC12 Cells; D051381:Rats; D022142:Secretory Vesicles / Q000187:drug effects / Q000378:metabolism*; D009435:Synaptic Transmission / Q000187:drug effects
chemical_list: D000077482:Carbon Fiber; D018765:Dopamine Uptake Inhibitors; D009627:Nomifensine; D002244:Carbon; D004298:Dopamine