title: Extracellular electron transfer across bacterial cell membranes via a cytocompatible redox-active polymer.
abstract: A redox-active phospholipid polymer with a phospholipid-mimicking structure (2-methacryloyloxyethyl phosphorylcholine; MPC) was synthesized to construct a biocompatible electron mediator between bacteria and an electrode. In this study, a copolymer of MPC and vinylferrocene [VF; poly(MPC-co-VF)] (PMF) is synthesized. When PMF is added to cultures of the bacterial species Escherichia coli (Gram negative) and Lactobacillus plantarum (Gram positive), which have different cell wall structures, a catalytic current mediated by PMF is observed. In addition, growth curves and live/dead assays indicate that PMF does not decrease metabolic activity or cell viability. These results indicate that PMF mediates extracellular electron transfer across bacterial cell membranes without associated cytotoxicity.
authors: Department of Applied Chemistry, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
authors: Koichi
authors: K
authors: Nishio
authors: Ryuhei
authors: R
authors: Nakamura
authors: Xiaojie
authors: X
authors: Lin
authors: Tomohiro
authors: T
authors: Konno
authors: Kazuhiko
authors: K
authors: Ishihara
authors: Shuji
authors: S
authors: Nakanishi
authors: Kazuhito
authors: K
authors: Hashimoto
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism*; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D004579:Electron Transport; D004926:Escherichia coli / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism*; D048191:Lactiplantibacillus plantarum / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D010084:Oxidation-Reduction; D011108:Polymers / Q000737:chemistry / Q000378:metabolism*
chemical_list: D001672:Biocompatible Materials; D011108:Polymers
keywords: cytotoxicity; electrochemistry; electron transfer; microbiology; phospholipids