title: Improvement of the compressive strength of a cuttlefish bone-derived porous hydroxyapatite scaffold via polycaprolactone coating.
abstract: Cuttlefish bones (CBs) have emerged as attractive biomaterials because of their porous structure and components that can be converted into hydroxyapatite (HAp) via a hydrothermal reaction. However, their brittleness and low strength restrict their application in bone tissue engineering. Therefore, to improve the compressive strength of the scaffold following hydrothermal conversion to a HAp form of CB (CB-HAp), the scaffold was coated using a polycaprolactone (PCL) polymer at various concentrations. In this study, raw CB was successfully converted into HAp via a hydrothermal reaction. We then evaluated their surface properties and composition by scanning electron microscopy and X-ray diffraction analysis. The CB-HAp coated with PCL showed improved compressive performance and retained a microporous structure. The compressive strength was significantly increased upon coating with 5 and 10% PCL, by 2.09- and 3.30-fold, respectively, as compared with uncoated CB-HAp. However, coating with 10% PCL resulted in a reduction in porosity. Furthermore, an in vitro biological evaluation demonstrated that MG-63 cells adhered well, proliferated and were able to be differentiated on the PCL-coated CB-HAp scaffold, which was noncytotoxic. These results suggest that a simple coating method is useful to improve the compressive strength of CB-HAp for bone tissue engineering applications.
authors: Wonkwang Bone Regeneration Research Institute, Wonkwang University, Iksan, 570-749, Korea; Bonecell Biotech Inc., 77, Dunsan-dong, Seo-gu, Daejeon, 302-830, Korea.
authors: Beom-Su
authors: BS
authors: Kim
authors: Hyo Jin
authors: HJ
authors: Kang
authors: Jun
authors: J
authors: Lee
mesh_terms: D000818:Animals; D002460:Cell Line; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D019245:Compressive Strength; D049832:Decapodiformes / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D011091:Polyesters / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D011091:Polyesters; C016240:polycaprolactone; D017886:Durapatite
keywords: bone tissue engineering; compressive strength; cuttlefish bone; hydrothermal reaction; hydroxyapatite; polycaprolactone