title: The therapeutic role of oral tolerance in dextran sulfate sodium-induced colitis via Th1-Th2 balance and γδ T cells.
abstract: OBJECTIVE
  To evaluate the state of oral tolerance and its therapeutic role in mice with dextran sulfate sodium (DSS)-induced colitis.
  
  
  METHODS
  Delayed-type hypersensitivity (DTH) was determined 7 and 14 days after DSS-induced colitis and control mice. Disease activity index (DAI) score and colonic histopathological score were measured 7 days after colonic extracted protein (CEP) or bovine serum albumin (BSA) (control) was administrated, with the evaluation of Th1-Th2 balance in the spleen, Peyer's patch and γδ T cells in intraepithelial lymphocytes and lamina proper lymphocytes in the intestine.
  
  
  RESULTS
  After fed with 250 μg ovalbumin oral tolerance was induced in 7 days in both DSS-induced colitis and control mice, while oral tolerance persisted in the control mice but vanished in DSS-induced colitis 14 days after ovalbumin challenge. DAI and colonic histopathological scores were decreased significantly after the ingestion of CEP (controlled by BSA) in DSS-induced colitis with significant reduction of Th1 and the ratio of Th1 to Th2 in Peyer's patch as well as the γδ T cells in lamina proper lymphocytes in the intestine. No significant difference in Th1-Th2 balance in the spleen and γδ T cells in intraepithelial lymphocytes in the intestine were observed.
  
  
  CONCLUSIONS
  There is a defect in oral tolerance at day 7 in DSS-induced colitis. If taken orally, CEP may have a protective role in DSS-induced colitis, which may be related to the deflection from Th1 to Th2 in Peyer's patch and the reduction of γδ T cells in lamina proper lymphocytes in the intestine.
authors: Department of Gastroenterology, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang Province, China.
authors: Min
authors: M
authors: Yue
authors: Zhe
authors: Z
authors: Shen
authors: Chao Hui
authors: CH
authors: Yu
authors: Hua
authors: H
authors: Ye
authors: You Ming
authors: YM
authors: Li
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001324:Autoantigens / Q000276:immunology*; D003093:Colitis, Ulcerative / Q000139:chemically induced / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D003106:Colon / Q000276:immunology / Q000473:pathology; D016264:Dextran Sulfate; D007108:Immune Tolerance / Q000276:immunology*; D018928:Immunity, Mucosal; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010581:Peyer's Patches / Q000276:immunology; D011506:Proteins / Q000276:immunology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000032:analysis; D013154:Spleen / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D058408:Th1-Th2 Balance
chemical_list: D001324:Autoantigens; D011506:Proteins; D016692:Receptors, Antigen, T-Cell, gamma-delta; D016264:Dextran Sulfate
keywords: T-lymphocytes; dextran sulfate; inflammatory bowel disease; oral tolerance; γδ T cell