title: Select interneuron clusters determine female sexual receptivity in Drosophila.
abstract: Female Drosophila with the spinster mutation repel courting males and rarely mate. Here we show that the non-copulating phenotype can be recapitulated by the elimination of spinster functions from either spin-A or spin-D neuronal clusters, in the otherwise wild-type (spinster heterozygous) female brain. Spin-D corresponds to the olfactory projection neurons with dendrites in the antennal lobe VA1v glomerulus that is fruitless-positive, sexually dimorphic and responsive to fly odour. Spin-A is a novel local neuron cluster in the suboesophageal ganglion, which is known to process contact chemical pheromone information and copulation-related signals. A slight reduction in spinster expression to a level with a minimal effect is sufficient to shut off female sexual receptivity if the dominant-negative mechanistic target of rapamycin is simultaneously expressed, although the latter manipulation alone has only a marginal effect. We propose that spin-mediated mechanistic target of rapamycin signal transduction in these neurons is essential for females to accept the courting male.
authors: Division of Neurogenetics, Tohoku University Graduate School of Life Sciences, Sendai 980-8577, Japan.
authors: Akira
authors: A
authors: Sakurai
authors: Masayuki
authors: M
authors: Koganezawa
authors: Kei-ichiro
authors: K
authors: Yasunaga
authors: Kazuo
authors: K
authors: Emoto
authors: Daisuke
authors: D
authors: Yamamoto
mesh_terms: D000818:Animals; D003307:Copulation / Q000187:drug effects; D029721:Drosophila Proteins / Q000378:metabolism; D004331:Drosophila melanogaster / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D005260:Female; D006579:Heterozygote; D006720:Homozygote; D007395:Interneurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D009457:Neuroglia / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D009833:Olfactory Pathways / Q000187:drug effects / Q000378:metabolism; D034622:RNA Interference / Q000187:drug effects; D018035:Receptors, Odorant / Q000378:metabolism; D012726:Sexual Behavior, Animal / Q000187:drug effects / Q000502:physiology*; D015398:Signal Transduction / Q000187:drug effects; D020123:Sirolimus / Q000494:pharmacology; D058570:TOR Serine-Threonine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: D029721:Drosophila Proteins; D008565:Membrane Proteins; C574157:Or47b protein, Drosophila; D018035:Receptors, Odorant; C429164:spin protein, Drosophila; D058570:TOR Serine-Threonine Kinases; D020123:Sirolimus
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