title: Oligodendrogliogenic and neurogenic adult subependymal zone neural stem cells constitute distinct lineages and exhibit differential responsiveness to Wnt signalling.
abstract: The adult mouse subependymal zone (SEZ) harbours adult neural stem cells (aNSCs) that give rise to neuronal and oligodendroglial progeny. However it is not known whether the same aNSC can give rise to neuronal and oligodendroglial progeny or whether these distinct progenies constitute entirely separate lineages. Continuous live imaging and single-cell tracking of aNSCs and their progeny isolated from the mouse SEZ revealed that aNSCs exclusively generate oligodendroglia or neurons, but never both within a single lineage. Moreover, activation of canonical Wnt signalling selectively stimulated proliferation within the oligodendrogliogenic lineage, resulting in a massive increase in oligodendrogliogenesis without changing lineage choice or proliferation within neurogenic clones. In vivo activation or inhibition of canonical Wnt signalling respectively increased or decreased the number of Olig2 and PDGFR- α positive cells, suggesting that this pathway contributes to the fine tuning of oligodendrogliogenesis in the adult SEZ.
authors: Department of Physiological Genomics, Institute of Physiology, Ludwig-Maximilians University Munich, Munich, Germany. ortegaf@uni-mainz.de
authors: Felipe
authors: F
authors: Ortega
authors: Sergio
authors: S
authors: Gascón
authors: Giacomo
authors: G
authors: Masserdotti
authors: Aditi
authors: A
authors: Deshpande
authors: Christiane
authors: C
authors: Simon
authors: Judith
authors: J
authors: Fischer
authors: Leda
authors: L
authors: Dimou
authors: D
authors: D
authors: Chichung Lie
authors: Timm
authors: T
authors: Schroeder
authors: Benedikt
authors: B
authors: Berninger
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism; D002453:Cell Cycle; D002454:Cell Differentiation; D002455:Cell Division; D019070:Cell Lineage; D049109:Cell Proliferation; D002478:Cells, Cultured; D002490:Central Nervous System / Q000196:embryology; D005260:Female; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000378:metabolism; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism*; D000076104:Oligodendrocyte Transcription Factor 2; D009836:Oligodendroglia / Q000166:cytology* / Q000378:metabolism*; D020796:Receptor, Platelet-Derived Growth Factor alpha / Q000378:metabolism; D060449:Wnt Signaling Pathway; D060508:Wnt3 Protein / Q000378:metabolism
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D009419:Nerve Tissue Proteins; D000076104:Oligodendrocyte Transcription Factor 2; D020796:Receptor, Platelet-Derived Growth Factor alpha; D060508:Wnt3 Protein; C512773:Olig2 protein, mouse; C555508:Wnt3 protein, mouse
references: Nat Protoc. 2011 Nov 17;6(12):1981-9
references: 22094733
references: Nat Protoc. 2008;3(6):935-40
references: 18536641
references: Science. 1994 May 20;264(5162):1145-8
references: 8178174
references: Science. 2009 Jul 10;325(5937):217-8
references: 19590005
references: J Neurosci. 2006 Jul 26;26(30):7907-18
references: 16870736
references: Stem Cells. 2009 Aug;27(8):2032-43
references: 19544429
references: Science. 1996 Feb 16;271(5251):978-81
references: 8584933
references: Neuron. 2002 Dec 19;36(6):1021-34
references: 12495619
references: Nat Neurosci. 2009 Dec;12(12):1524-33
references: 19881504
references: J Neurosci. 2010 Oct 6;30(40):13367-72
references: 20926663
references: Nat Neurosci. 2005 Jul;8(7):865-72
references: 15951811
references: Cell Tissue Res. 2008 Jan;331(1):193-210
references: 17828608
references: J Neurosci. 2008 Jan 9;28(2):434-46
references: 18184786
references: Proc Natl Acad Sci U S A. 2009 Apr 14;106(15):6387-92
references: 19332781
references: Brain Res Rev. 2011 Jun 24;67(1-2):147-56
references: 21236296
references: Glia. 2006 Jul;54(1):21-34
references: 16652340
references: Development. 2008 Jan;135(1):11-22
references: 18032449
references: J Neurosci. 2009 Apr 1;29(13):4172-88
references: 19339612
references: Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):13211-6
references: 12235363
references: J Neurocytol. 2002 Jul-Aug;31(6-7):437-55
references: 14501215
references: Nat Neurosci. 2010 May;13(5):541-550
references: 20418875
references: Genesis. 2012 Jun;50(6):506-15
references: 22173870
references: Nat Methods. 2011 Apr;8(4 Suppl):S30-5
references: 21451514
references: Neuron. 2011 Jul 28;71(2):250-62
references: 21791285
references: Curr Biol. 2001 Jun 26;11(12):951-61
references: 11448771
references: J Neurosci. 2011 Sep 28;31(39):13921-35
references: 21957254
references: Neuron. 2003 Oct 30;40(3):485-99
references: 14642274
references: PLoS Biol. 2010 May 18;8(5):e1000373
references: 20502524
references: Stem Cells. 2010 Feb;28(2):297-307
references: 19960516
references: Nat Neurosci. 2003 Nov;6(11):1127-34
references: 14583753
references: Mol Cell Neurosci. 2009 Nov;42(3):255-65
references: 19619658
references: Circ Res. 2006 Jun 23;98(12):1547-54
references: 16709902
references: Cereb Cortex. 2012 Oct;22(10):2415-27
references: 22095214
references: PLoS One. 2011;6(11):e27628
references: 22110700
references: PLoS One. 2010 Feb 23;5(2):e9370
references: 20186325
references: Nat Protoc. 2011 Nov 03;6(12):1847-59
references: 22051798
references: Semin Cell Dev Biol. 2009 Aug;20(6):744-50
references: 19374951
references: Development. 2011 Mar;138(6):1057-68
references: 21343361
references: Annu Rev Neurosci. 2009;32:149-84
references: 19555289
references: J Neurosci. 2008 Oct 8;28(41):10434-42
references: 18842903
references: Methods. 2001 Dec;25(4):402-8
references: 11846609
references: Cell Stem Cell. 2008 Sep 11;3(3):265-78
references: 18786414
references: Nature. 2005 Oct 27;437(7063):1370-5
references: 16251967
references: Cell Stem Cell. 2010 Dec 3;7(6):744-58
references: 21112568
references: Neurosci Res. 2006 Jul;55(3):223-33
references: 16621079
references: Cell. 1999 Jun 11;97(6):703-16
references: 10380923
references: Science. 2007 Jul 20;317(5836):381-4
references: 17615304
references: J Neurosci. 2008 Jun 18;28(25):6439-52
references: 18562615
references: Glia. 2011 Jun;59(6):869-81
references: 21446038
references: Neuron Glia Biol. 2007 Aug;3(3):209-20
references: 18634612
references: Neuron. 2011 Mar 10;69(5):918-29
references: 21382552
references: Neuron. 2011 May 26;70(4):674-86
references: 21609824
references: Mol Cell Neurosci. 2005 Nov;30(3):291-303
references: 16169246
references: Development. 1992 Jun;115(2):535-51
references: 1425339
references: Nature. 2009 Feb 12;457(7231):896-900
references: 19212410