title: Antioxidative carbohydrate polymer from Enhydra fluctuans and its interaction with bovine serum albumin.
abstract: Enhydra fluctuans leaves are traditionally sold on Indian markets for various health benefits. However, no phytochemical study on its high molecular weight compound has so far been performed. Chemical, chromatographic, ESI-TOF-MS, and NMR analyses of the water extracted carbohydrate polymer (CP) of E. fluctuans leaves showed the presence of a 24 kDa arabinogalactan having a (1,3)-linked β-d-Galp main chain, substituted at O-6 by (1,6)-linked β-d-Galp side chains. The latter residues were substituted at O-3 by (1,3)-, (1,5)-, and (1,3,5)-linked α-l-Araf chains, and nonreducing end-units of α-l-Araf and β-d-Galp. This polymer contained esterified phenolic acids. Biochemical analysis revealed similarity in antioxidative potential between the identified carbohydrate polymer and known standard antioxidants. The highly branched side chains and the phenolic acid residues of the arabinogalactan might be the functional sites. Fluorimetric and ultraviolet spectrometric analyses showed that the studied carbohydrate polymer can form complex with bovine serum albumin having binding constant K = 2.42 × 10(6)/M and changes its microenvironment. Thus, traditional aqueous extraction method provides a carbohydrate polymer, which stimulates a fair biological response: this could represent an interesting approach in phytotherapeutic treatments.
authors: Natural Products Laboratory, Department of Chemistry, The University of Burdwan , West Bengal 713 104, India.
authors: Debjani
authors: D
authors: Ghosh
authors: Sayani
authors: S
authors: Ray
authors: Kanika
authors: K
authors: Ghosh
authors: Valérie
authors: V
authors: Micard
authors: Udipta R
authors: UR
authors: Chatterjee
authors: Pradyot K
authors: PK
authors: Ghosal
authors: Bimalendu
authors: B
authors: Ray
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D019659:Asteraceae / Q000737:chemistry*; D002241:Carbohydrates / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D011108:Polymers / Q000737:chemistry*; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013329:Structure-Activity Relationship
chemical_list: D000975:Antioxidants; D002241:Carbohydrates; D011108:Polymers; D012710:Serum Albumin, Bovine