title: Upregulation of triglyceride synthesis in skeletal muscle overexpressing DGAT1.
abstract: The gene encoding diacylglycerol acyltransferase (DGAT1) is a functional and positional candidate gene for milk and intramuscular fat content. A bovine DGAT1 overexpression vector was constructed containing mouse MCK promoter and bovine DGAT1 cDNA. MCK-DGAT1 transgene in FVB mice was researched in present study. The transgene DGAT1 had a high level of expression in contrast to the endogenous DGAT1 in posterior tibial muscle of the transgenic mice, but a low expression level in the cardiac muscle. Compared with wild-type mice, triglyceride and DGAT1 content were approximately fourfold and 50% increased in posterior tibial muscle of the transgenic mice, respectively, while a little increase in cardiac muscle.
authors: Department of Animal Science, TianJin Agricultural University, TianJin 300384, P.R. China.
authors: Feifei
authors: F
authors: Yang
authors: Zhuying
authors: Z
authors: Wei
authors: Xiangbin
authors: X
authors: Ding
authors: Xinfeng
authors: X
authors: Liu
authors: Xiuguo
authors: X
authors: Ge
authors: Guimin
authors: G
authors: Song
authors: Guangpeng
authors: G
authors: Li
authors: Hong
authors: H
authors: Guo
mesh_terms: D000818:Animals; D002417:Cattle; D018076:DNA, Complementary / Q000235:genetics; D051048:Diacylglycerol O-Acyltransferase / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D051379:Mice; D008822:Mice, Transgenic; D018482:Muscle, Skeletal / Q000201:enzymology*; D014280:Triglycerides / Q000096:biosynthesis*
chemical_list: D018076:DNA, Complementary; D014280:Triglycerides; D051048:Diacylglycerol O-Acyltransferase
references: Mol Cell Biol. 1986 Aug;6(8):2855-64
references: 3785216
references: Anim Genet. 2003 Oct;34(5):354-7
references: 14510671
references: Mol Cell Biol. 1989 Aug;9(8):3393-9
references: 2796990
references: Mol Cell Biol. 1996 Apr;16(4):1649-58
references: 8657140
references: Science. 1981 Jan 30;211(4481):448-52
references: 6450446
references: Nat Genet. 2000 May;25(1):87-90
references: 10802663
references: Mol Cell Biol. 1988 Jan;8(1):62-70
references: 3336366
references: J Clin Invest. 2007 Jun;117(6):1679-89
references: 17510710
references: Diabetes. 2009 Nov;58(11):2516-24
references: 19675136
references: Chin Med J (Engl). 2004 Jun;117(6):893-8
references: 15198894
references: Proc Natl Acad Sci U S A. 1998 Oct 27;95(22):13018-23
references: 9789033
references: Proc Natl Acad Sci U S A. 2002 Jul 9;99(14):9300-5
references: 12077321