title: Isolation and characterization of a rare waterborne lytic phage of Salmonella enterica serovar Paratyphi B.
abstract: A lytic phage of Salmonella serovar Paratyphi B, named φSPB, was isolated from surface waters of the Pavana River in India. Phage φSPB is a member of the Podoviridae family and is morphologically similar to the 7-11 phages of the C3 morphotype of tailed phages, characterized by a very long, cigar-shaped head. The head measured approximately 153 × 57 nm, and the tail size was 12 × 7 nm. The phage was stable over a wide range of pH (4-9) and temperature (4-40 °C). The adsorption rate constant was 4.7 × 10(-10). Latent and eclipse periods were 10 and 15 min, respectively, and the burst size was 100 plaque-forming units/infected cell after 25 min at 37 °C. The phage DNA was 59 kb in size. Ten major proteins were observed on SDS-PAGE, although some of these proteins could be bacterial contaminants. This is the first report of Salmonella enterica subsp. enterica serovar Paratyphi B phage of C3 morphotype from India that has many unique features, such as high replication potential, short replication time, and stability over a wide range of pH and temperature, making it a promising biocontrol agent against the drug-resistant strains of Salmonella Paratyphi B.
authors: a Department of Environmental Sciences, University of Pune, Pune 411 007, India.
authors: Sangeeta S
authors: SS
authors: Ahiwale
authors: Ashok V
authors: AV
authors: Bankar
authors: Sujata N
authors: SN
authors: Tagunde
authors: Smita
authors: S
authors: Zinjarde
authors: Hans W
authors: HW
authors: Ackermann
authors: B P
authors: BP
authors: Kapadnis
mesh_terms: D007194:India; D017902:Podoviridae / Q000737:chemistry / Q000254:growth & development / Q000302:isolation & purification* / Q000502:physiology*; D045483:Rivers / Q000821:virology*; D012483:Salmonella Phages / Q000737:chemistry / Q000254:growth & development / Q000302:isolation & purification* / Q000502:physiology*; D012484:Salmonella paratyphi B / Q000821:virology*; D013696:Temperature