title: [Effect of Staphylococcus aureus on the expressions of TLR2, IL-1β, TNF-α and NF-κB in Bcap-37 cells].
abstract: OBJECTIVE
  To observe the effects of Staphylococcus aureus (S.aureus) on the expressions of Toll-like receptor 2 (TLR2), proinflammatory cytokines (IL-1β, TNF-α) and nuclear factor κB(NF-κB) in human breast cancer Bcap-37 cells in vitro.
  
  
  METHODS
  At the different time points after Bcap-37 cells were challenged with S.aureus, the expressions of TLR2 mRNA and protein were respectively detected using RT-PCR and flow cytometry, the concentrations of IL-1β and TNF-α were examined by ELISA and NF-κB level by Western blotting.
  
  
  RESULTS
  The mRNA and protein levels of TLR2 gradually increased in Bcap-37 cells since the stimulations of S.aureus and then dropped after the levels peaked at 4 and 6 hours, respectively (P<0.05). Meanwhile, the expressions of IL-1β and TNF-α was significantly elevated when Bcap-37 cells were exposed to S.aureus, and reached the maximal levels at 12 and 16 hours, respectively (P<0.05), and then IL-1β went down. Expression of NF-κB rose in a time-dependent manner after stimulated by S.aureus up to the maximum at 12 hours, and declined after 14 hours.
  
  
  CONCLUSIONS
  In Bcap-37 cells, S.aureus exposure could up-regulate the expression of TLR2 at both mRNA and protein levels, increase the production of IL-1β and TNF-α, and stimulate the expression of NF-κB.
authors: Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China. zalxju@gmail.com
authors: Ailian
authors: A
authors: Zhang
authors: Yankun
authors: Y
authors: Yu
authors: Fupei
authors: F
authors: Liu
authors: Danyang
authors: D
authors: Wang
authors: Hao
authors: H
authors: Zhou
authors: Fuchun
authors: F
authors: Zhang
mesh_terms: D002460:Cell Line; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D053583:Interleukin-1beta / Q000235:genetics* / Q000378:metabolism; D008413:Mastitis / Q000235:genetics / Q000378:metabolism; D016328:NF-kappa B / Q000235:genetics* / Q000378:metabolism; D013203:Staphylococcal Infections / Q000235:genetics / Q000378:metabolism; D013211:Staphylococcus aureus / Q000502:physiology*; D013997:Time Factors; D051195:Toll-Like Receptor 2 / Q000235:genetics* / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000378:metabolism
chemical_list: D053583:Interleukin-1beta; D016328:NF-kappa B; D051195:Toll-Like Receptor 2; D014409:Tumor Necrosis Factor-alpha