title: Dual-specific interaction to detect DNA on gold nanoparticles.
abstract: An approach to selectively and efficiently detect single strand DNA is developed by using streptavidin coated gold nanoparticles (StAuNPs) as efficient quenchers. The central concept for the successful detection is the combination the of streptavidin-biotin interaction with specific probe-target DNA hybridization. Biotin labeled probe DNAs act as "bridges" to bring Cy5 labeled targets to the particle surface and the fluorophore dye can be rapidly and efficiently quenched by StAuPNs. By measuring the changes of photoluminescence intensity of Cy5, an efficient, selective, and reversed detection of DNA hybridization is realized. The methodology may pave a new way for simple and rapid detections of biomolecules.
authors: State Key Laboratory of Metal Matrix Composites, School of Materials Science & Engineering, Shanghai Jiao Tong University, Shanghai 200240, China. clfeng@sjtu.edu.cn
authors: Chuan-Liang
authors: CL
authors: Feng
authors: Xiao-Qiu
authors: XQ
authors: Dou
authors: Qing-Lei
authors: QL
authors: Liu
authors: Wang
authors: W
authors: Zhang
authors: Jia-Jun
authors: JJ
authors: Gu
authors: Shen-Min
authors: SM
authors: Zhu
authors: Andrew Tobias Aveling
authors: AT
authors: Jenkins
authors: Di
authors: D
authors: Zhang
mesh_terms: D002232:Carbocyanines / Q000378:metabolism; D004247:DNA / Q000032:analysis*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D019809:Streptavidin / Q000378:metabolism
chemical_list: D002232:Carbocyanines; D012996:Solutions; C085321:cyanine dye 5; D006046:Gold; D004247:DNA; D019809:Streptavidin
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