title: Superficial collagen fibril modulus and pericellular fixed charge density modulate chondrocyte volumetric behaviour in early osteoarthritis.
abstract: The aim of this study was to investigate if the experimentally detected altered chondrocyte volumetric behavior in early osteoarthritis can be explained by changes in the extracellular and pericellular matrix properties of cartilage. Based on our own experimental tests and the literature, the structural and mechanical parameters for normal and osteoarthritic cartilage were implemented into a multiscale fibril-reinforced poroelastic swelling model. Model simulations were compared with experimentally observed cell volume changes in mechanically loaded cartilage, obtained from anterior cruciate ligament transected rabbit knees. We found that the cell volume increased by 7% in the osteoarthritic cartilage model following mechanical loading of the tissue. In contrast, the cell volume decreased by 4% in normal cartilage model. These findings were consistent with the experimental results. Increased local transversal tissue strain due to the reduced collagen fibril stiffness accompanied with the reduced fixed charge density of the pericellular matrix could increase the cell volume up to 12%. These findings suggest that the increase in the cell volume in mechanically loaded osteoarthritic cartilage is primarily explained by the reduction in the pericellular fixed charge density, while the superficial collagen fibril stiffness is suggested to contribute secondarily to the cell volume behavior.
authors: Department of Applied Physics, University of Eastern Finland, P.O. Box 1627, 70211 Kuopio, Finland. petri.tanska@uef.fi
authors: Petri
authors: P
authors: Tanska
authors: Siru M
authors: SM
authors: Turunen
authors: Sang Kuy
authors: SK
authors: Han
authors: Petro
authors: P
authors: Julkunen
authors: Walter
authors: W
authors: Herzog
authors: Rami K
authors: RK
authors: Korhonen
mesh_terms: D000818:Animals; D000070598:Anterior Cruciate Ligament Injuries; D048429:Cell Size; D019902:Chondrocytes / Q000378:metabolism* / Q000473:pathology*; D003094:Collagen / Q000737:chemistry*; D019295:Computational Biology; D004195:Disease Models, Animal; D055119:Elastic Modulus; D005109:Extracellular Matrix / Q000378:metabolism / Q000473:pathology; D020342:Finite Element Analysis; D008954:Models, Biological; D010003:Osteoarthritis / Q000209:etiology / Q000378:metabolism* / Q000473:pathology*; D011817:Rabbits; D055672:Static Electricity; D013314:Stress, Mechanical
chemical_list: D003094:Collagen
references: J Biomech. 2008;41(2):480-5
references: 17936762
references: J Biomech. 2005 Mar;38(3):509-17
references: 15652549
references: Scand J Med Sci Sports. 2000 Aug;10(4):186-98
references: 10898262
references: Biorheology. 1981;18(3-6):619-32
references: 6799013
references: J Biomech. 2007;40(8):1862-70
references: 17052722
references: Osteoarthritis Cartilage. 2006 Sep;14(9):889-97
references: 16626979
references: Microsc Res Tech. 2008 Apr;71(4):279-87
references: 18072283
references: Osteoarthritis Cartilage. 2013 Mar;21(3):505-13
references: 23247212
references: Med Biol Eng Comput. 2009 Dec;47(12):1281-90
references: 19898885
references: J Anat. 1997 Jul;191 ( Pt 1):1-13
references: 9279653
references: Biomech Model Mechanobiol. 2012 May;11(5):655-64
references: 21847614
references: Biorheology. 2002;39(1-2):39-45
references: 12082265
references: Arch Biochem Biophys. 1998 Mar 15;351(2):207-19
references: 9515057
references: J Orthop Res. 1995 May;13(3):410-21
references: 7602402
references: Histochemistry. 1985;82(3):249-55
references: 2581923
references: Biopolymers. 2001;62(1):1-8
references: 11135186
references: Biochim Biophys Acta. 2006 Jul;1758(7):934-41
references: 16815242
references: Biomech Model Mechanobiol. 2013 Jun;12(3):417-29
references: 22710890
references: J Biomech Eng. 2003 Jun;125(3):323-33
references: 12929236
references: J Cell Sci. 1995 Apr;108 ( Pt 4):1497-508
references: 7615670
references: Nature. 1976 Apr 29;260(5554):808-9
references: 1264261
references: Ann N Y Acad Sci. 2006 Apr;1068:498-512
references: 16831947
references: Osteoarthritis Cartilage. 2010 Jan;18(1):73-81
references: 19733642
references: Ann Rheum Dis. 1998 Apr;57(4):237-45
references: 9709181
references: Osteoarthritis Cartilage. 1999 Jan;7(1):59-70
references: 10367015
references: Biorheology. 2002;39(1-2):133-43
references: 12082276
references: Osteoarthritis Cartilage. 2006 Jun;14(6):554-60
references: 16476555
references: J Biomech Eng. 2008 Apr;130(2):021003
references: 18412490
references: Arthritis Rheum. 1995 Apr;38(4):525-32
references: 7718006
references: Arthritis Rheum. 1984 Apr;27(4):388-97
references: 6712755
references: Biomech Model Mechanobiol. 2003 Aug;2(1):3-19
references: 14586814
references: Osteoarthritis Cartilage. 2009 Apr;17(4):448-55
references: 18849174
references: J Bone Joint Surg Am. 1971 Apr;53(3):523-37
references: 5580011
references: J Bone Joint Surg Am. 1981 Jan;63(1):131-9
references: 7451514
references: J Cell Physiol. 1993 Feb;154(2):262-70
references: 8425907
references: Mol Cell Biomech. 2005 Dec;2(4):191-204
references: 16705865
references: J Cell Sci. 1996 Feb;109 ( Pt 2):499-508
references: 8838673
references: Histochem J. 1997 Apr;29(4):317-27
references: 9184847
references: Arthritis Rheum. 2001 Apr;44(4):846-55
references: 11315924
references: Histochem J. 1996 Aug;28(8):577-90
references: 8894661
references: Proc Inst Mech Eng H. 1997;211(2):209-11
references: 9184461
references: Biochim Biophys Acta. 1989 Jul 21;992(1):59-65
references: 2752039
references: J Biomech Eng. 2009 Apr;131(4):044504
references: 19275446
references: Comput Methods Biomech Biomed Engin. 2011 Jul;14(7):657-64
references: 20665295
references: J Cell Sci. 1998 Mar;111 ( Pt 5):573-83
references: 9454731
references: J Orthop Res. 1994 Jul;12(4):474-84
references: 8064478
references: J Biomech Eng. 1991 Aug;113(3):245-58
references: 1921350
references: J R Soc Interface. 2010 Jun 6;7(47):895-903
references: 19933220
references: Cells Tissues Organs. 2003;175(3):121-32
references: 14663155
references: Med Eng Phys. 2009 Oct;31(8):1038-42
references: 19586793
references: Osteoarthritis Cartilage. 1996 Jun;4(2):87-98
references: 8806111
references: Biophys J. 2002 Feb;82(2):720-7
references: 11806914
references: J Cell Physiol. 2005 Jul;204(1):309-19
references: 15668989
references: J Orthop Res. 1997 Mar;15(2):197-203
references: 9167621
references: J Biomech. 2005 Jun;38(6):1195-204
references: 15863103