title: Highly fluorescent and biocompatible iridium nanoclusters for cellular imaging.
abstract: Highly fluorescent iridium nanoclusters were synthesized and investigated its application as a potential intracellular marker. The iridium nanoclusters were prepared with an average size of ~2 nm. Further, these nanoclusters were refluxed with aromatic ligands, such as 2,2'-binaphthol (BINOL) in order to obtain fluorescence properties. The photophysical properties of these bluish-green emitting iridium nanoclusters were well characterized by using UV-Visible, fluorescence and lifetime decay measurements. The emission spectrum for these nanoclusters exhibit three characteristic peaks at 449, 480 and 515 nm. The fluorescence quantum yield of BINOL-Ir NCs were estimated to be 0.36 and the molar extinction co-efficients were in the order of 10(6) M(-1)cm(-1). In vitro cytotoxicity studies in HeLa cells reveal that iridium nanoclusters exhibited good biocompatibility with an IC50 value of ~100 μg/ml and also showed excellent co-localization and distribution throughout the cytoplasm region without entering into the nucleus. This research has opened a new window in developing the iridium nanoparticle based intracellular fluorescent markers and has wide scope to act as biomedical nanocarrier to carry many biological molecules and anticancer drugs.
authors: Department of Chemistry, National Tsing Hua University, Hsinchu, 30013, Taiwan, Republic of China. vankayalaraviraj999@gmail.com
authors: Raviraj
authors: R
authors: Vankayala
authors: Ganesh
authors: G
authors: Gollavelli
authors: Badal Kumar
authors: BK
authors: Mandal
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000493:pharmacokinetics; D002477:Cells / Q000378:metabolism / Q000648:ultrastructure; D005453:Fluorescence; D006367:HeLa Cells; D006801:Humans; D007495:Iridium / Q000737:chemistry* / Q000493:pharmacokinetics; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000737:chemistry*; D008954:Models, Biological; D057054:Molecular Imaging / Q000295:instrumentation* / Q000379:methods; D061848:Optical Imaging / Q000295:instrumentation / Q000379:methods; D014018:Tissue Distribution; D014407:Tumor Cells, Cultured
chemical_list: D001672:Biocompatible Materials; D007495:Iridium
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