title: A simple shotgun proteomics method for rapid bacterial identification.
abstract: Bacterial pathogens were rapidly identified by shotgun proteomics using a novel, easy to implement database search strategy. Peptide sequence data from nano-LC-MS/MS was searched against a database represented by concatenated proteomes of completed genome sequences. Select bacterial species, including BSL-3 select agents, were used to demonstrate this method.
authors: National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba R3E 3R2, Canada.
authors: Dobryan M
authors: DM
authors: Tracz
authors: Stuart J
authors: SJ
authors: McCorrister
authors: Patrick M
authors: PM
authors: Chong
authors: David M
authors: DM
authors: Lee
authors: Cindi R
authors: CR
authors: Corbett
authors: Garrett R
authors: GR
authors: Westmacott
mesh_terms: D001419:Bacteria / Q000737:chemistry* / Q000145:classification*; D001426:Bacterial Proteins / Q000032:analysis*; D001431:Bacteriological Techniques / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods; D030541:Databases, Genetic; D020543:Proteome / Q000032:analysis; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D001426:Bacterial Proteins; D020543:Proteome