title: Puf mediates translation repression of transmission-blocking vaccine candidates in malaria parasites.
abstract: Translational control of gene expression plays an essential role in development. In malaria parasites, translational regulation is critical during the development of specialized transition stages between the vertebrate host and mosquito vector. Here we show that a Pumilio/FBF (Puf) family RNA-binding protein, PfPuf2, is required for the translation repression of a number of transcripts in gametocytes including two genes encoding the transmission-blocking vaccine candidates Pfs25 and Pfs28. Whereas studies to date support a paradigm of Puf-mediated translation regulation through 3' untranslated regions (UTRs) of target mRNAs, this study, for the first time, identifies a functional Puf-binding element (PBE) in the 5'UTR of pfs25. We provide both in vitro and in vivo evidence to demonstrate that PfPuf2 binds to the PBEs in pfs25 and pfs28 to mediate translation repression. This finding provides a renewed view of Pufs as versatile translation regulators and sheds light on their functions in the development of lower branches of eukaryotes.
authors: Department of Entomology, Pennsylvania State University, University Park, Pennsylvania, United States of America.
authors: Jun
authors: J
authors: Miao
authors: Qi
authors: Q
authors: Fan
authors: Daniel
authors: D
authors: Parker
authors: Xiaolian
authors: X
authors: Li
authors: Jianyong
authors: J
authors: Li
authors: Liwang
authors: L
authors: Cui
mesh_terms: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000818:Animals; D005786:Gene Expression Regulation; D017780:Malaria Vaccines / Q000276:immunology*; D010963:Plasmodium falciparum / Q000235:genetics* / Q000254:growth & development* / Q000276:immunology / Q000378:metabolism; D011485:Protein Binding; D014176:Protein Biosynthesis; D015800:Protozoan Proteins / Q000235:genetics* / Q000378:metabolism*; D016053:RNA, Protozoan / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D012045:Regulatory Sequences, Nucleic Acid; D014158:Transcription, Genetic
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D017780:Malaria Vaccines; D015800:Protozoan Proteins; D016053:RNA, Protozoan; D016601:RNA-Binding Proteins
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