title: Virome profiling of bats from Myanmar by metagenomic analysis of tissue samples reveals more novel Mammalian viruses.
abstract: Bats are reservoir animals harboring many important pathogenic viruses and with the capability of transmitting these to humans and other animals. To establish an effective surveillance to monitor transboundary spread of bat viruses between Myanmar and China, complete organs from the thorax and abdomen from 853 bats of six species from two Myanmar counties close to Yunnan province, China, were collected and tested for their virome through metagenomics by Solexa sequencing and bioinformatic analysis. In total, 3,742,314 reads of 114 bases were generated, and over 86% were assembled into 1,649,512 contigs with an average length of 114 bp, of which 26,698 (2%) contigs were recognizable viral sequences belonging to 24 viral families. Of the viral contigs 45% (12,086/26,698) were related to vertebrate viruses, 28% (7,443/26,698) to insect viruses, 27% (7,074/26,698) to phages and 95 contigs to plant viruses. The metagenomic results were confirmed by PCR of selected viruses in all bat samples followed by phylogenetic analysis, which has led to the discovery of some novel bat viruses of the genera Mamastrovirus, Bocavirus, Circovirus, Iflavirus and Orthohepadnavirus and to their prevalence rates in two bat species. In conclusion, the present study aims to present the bat virome in Myanmar, and the results obtained further expand the spectrum of viruses harbored by bats.
authors: Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, Jilin, China.
authors: Biao
authors: B
authors: He
authors: Zuosheng
authors: Z
authors: Li
authors: Fanli
authors: F
authors: Yang
authors: Junfeng
authors: J
authors: Zheng
authors: Ye
authors: Y
authors: Feng
authors: Huancheng
authors: H
authors: Guo
authors: Yingying
authors: Y
authors: Li
authors: Yiyin
authors: Y
authors: Wang
authors: Nan
authors: N
authors: Su
authors: Fuqiang
authors: F
authors: Zhang
authors: Quanshui
authors: Q
authors: Fan
authors: Changchun
authors: C
authors: Tu
mesh_terms: D000818:Animals; D001435:Bacteriophages / Q000235:genetics*; D002685:Chiroptera / Q000821:virology*; D004267:DNA Viruses / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D004199:Disease Vectors; D016679:Genome, Viral; D059014:High-Throughput Nucleotide Sequencing; D054892:Metagenome; D008969:Molecular Sequence Data; D002052:Myanmar; D010802:Phylogeny; D058974:Phylogeography; D010942:Plant Viruses / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012328:RNA Viruses / Q000235:genetics*; D017422:Sequence Analysis, DNA; D015047:Zoonoses
chemical_list: D004279:DNA, Viral
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