title: [The role of sinomenine in treatment of allergic rhinitis mice model and its mechanism].
abstract: OBJECTIVE
  To explore the role of sinomenine in treatment of allergic rhinitis mice model and its possible mechanism.
  
  
  METHODS
  We used ovalbumin (OVA) to make allergic rhinitis model of BALB/c mice. Saline was used in the control group. When we challenged the mice with OVA intranasally, the mice in sinomenine treatment group were feed by the food containing sinomenine. Mice were then killed 24 h after the last OVA challenge. The noses of mice from each group were removed en bloc and fixed, then each section was stained with hematoxylin and eosin. ELISA assay was used to measure the concentration of anti-OVA IgE, IL-4 and IFN-gamma. The proteins expressive level of T-bet and GATA3 were examined.
  
  
  RESULTS
  Nasal mucosa of the mice in sinomenine treatment group were not hyperplasia and without obvious infiltration of eosinophils. The concentration of anti-OVA IgE, IL-4 and IFN-gamma in the serum and T-bet and GATA3 expression levels of sinomenine treatment group were lower than those of allergic rhinitis group.
  
  
  CONCLUSIONS
  The sinomenine can be used to treat allergic rhinitis mice, and the mechanism may rely on the improvements of the Th1/Th2 imbalance.
authors: Department of Otolaryngology-Head and Neck Surgery, People's Hospital of Wuhan University, Wuhan, 430060, China.
authors: Zhe
authors: Z
authors: Chen
authors: Zezhang
authors: Z
authors: Tao
authors: Nana
authors: N
authors: Zhang
authors: Jie
authors: J
authors: Ren
authors: Yuqin
authors: Y
authors: Deng
authors: Bakui
authors: B
authors: Xiao
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004804:Eosinophils / Q000378:metabolism; D050990:GATA3 Transcription Factor / Q000378:metabolism; D007073:Immunoglobulin E / Q000097:blood; D007371:Interferon-gamma / Q000097:blood; D015847:Interleukin-4 / Q000097:blood; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009019:Morphinans / Q000627:therapeutic use*; D009297:Nasal Mucosa / Q000473:pathology; D008517:Phytotherapy; D065631:Rhinitis, Allergic; D012221:Rhinitis, Allergic, Perennial / Q000097:blood / Q000188:drug therapy*; D020825:T-Box Domain Proteins / Q000378:metabolism; D018417:Th1 Cells; D018418:Th2 Cells; D000098815:T-bet Transcription Factor
chemical_list: D050990:GATA3 Transcription Factor; C494748:Gata3 protein, mouse; D009019:Morphinans; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D015847:Interleukin-4; D007073:Immunoglobulin E; C009271:sinomenine; D007371:Interferon-gamma