title: Complete denture biofilm after brushing with specific denture paste, neutral soap and artificial saliva.
abstract: This study compared the levels of biofilm in maxillary and mandibular complete dentures and evaluated the number of colony-forming units (cfu) of yeasts, after using auxiliary brushing agents and artificial saliva. Twenty-three denture wearers with hyposalivation and xerostomia were instructed to brush the dentures 3 times a day during 3 weeks with the following products: Corega Brite denture dentifrice, neutral liquid soap, Corega Brite combined with Oral Balance (artificial saliva) or tap water. For biofilm quantification, the internal surfaces of the dentures were disclosed, photographed and measured using a software. For microbiological analysis, the biofilm was scrapped off, and the harvested material was diluted, sown in CHROMagar™ Candida and incubated at 37°C for 48 h. Data were analyzed statistically by two-way ANOVA and Tukey's test (α=0.05). Mandibular dentures presented a mean biofilm percentage (µ=26.90 ± 21.10) significantly greater than the maxillary ones (µ=18.0 ± 15.0) (p<0.05). Brushing using Corega Brite combined with Oral Balance (µ=15.87 ± 18.47) was more effective (p<0.05) than using the denture dentifrice (µ=19.47 ± 17.24), neutral soap (µ=23.90 ± 18.63) or tap water (control; µ=32.50 ± 20.68). For the microbiological analysis, the chi-square test did not indicate significant difference between the hygiene products for either type of denture. The more frequently isolated species of yeasts were C. albicans, C. tropicalis and C. glabrata. In conclusion, mandibular dentures had more biofilm formation than maxillary ones. Denture brushing with Corega Brite dentifrice combined with the use of Oral Balance was the most effective method for reduction of biofilm levels, but the use of products did not show difference in yeast cfu counts.
authors: Department of Dental Materials and Prosthodontics, Ribeirão Preto Dental School, University of São Paulo, Ribeirão Preto, SP, Brazil. helenpar@forp.usp.br
authors: Helena de Freitas Oliveira
authors: Hde F
authors: Paranhos
authors: Antônio Eduardo Sparça
authors: AE
authors: Salles
authors: Leandro Dorigan de
authors: LD
authors: Macedo
authors: Cláudia Helena da
authors: CH
authors: Silva-Lovato
authors: Valéria Oliveira
authors: VO
authors: Pagnano
authors: Evandro
authors: E
authors: Watanabe
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000704:Analysis of Variance; D018441:Biofilms; D002175:Candida / Q000302:isolation & purification; D015169:Colony Count, Microbial; D018592:Cross-Over Studies; D003820:Denture Cleansers; D003824:Denture, Complete / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012464:Saliva, Artificial; D012915:Soaps; D018709:Statistics, Nonparametric; D014099:Toothbrushing; D014987:Xerostomia
chemical_list: D003820:Denture Cleansers; D012464:Saliva, Artificial; D012915:Soaps; C540407:corega tabs