title: The periodontopathogenic bacterium Eikenella corrodens produces an autoinducer-2-inactivating enzyme.
abstract: Eikenella corrodens produces autoinducer-2 (AI-2) in the mid log phase, and AI-2 activity decreases dramatically during the stationary phase. We investigated the mechanism underlying this decrease in AI-2 activity. To analyze the mechanism, we extracted and purified AI-2 from the supernatant of mid-log-phase culture. Simultaneously, the stationary-phase culture supernatant was fractionated by ammonium sulfate precipitation. On incubating purified AI-2 and 4-hydroxy-5-methyl-3(2H)-furanone (MHF) with each fraction, the 30% fraction decreased both AI-2 and MHF activities. The data suggest that AI-2 and MHF were rendered inactive in the same manner. Heat and/or trypsin treatment of the 30% fraction did not completely arrest AI-2 inactivation, suggesting that partially heat-stable proteins are involved in AI-2 inactivation. We observed that an enzyme converted MHF to another form. This suggests that E. corrodens produces an AI-2 inactivating enzyme, and that AI-2 can be degraded or modified by it.
authors: Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yoshida, Yamaguchi, Japan.
authors: Mohammad Minnatul
authors: MM
authors: Karim
authors: Ayako
authors: A
authors: Nagao
authors: Fariha Jasin
authors: FJ
authors: Mansur
authors: Tetsuro
authors: T
authors: Matsunaga
authors: Yoshihiko
authors: Y
authors: Akakabe
authors: Yuichiro
authors: Y
authors: Noiri
authors: Shigeyuki
authors: S
authors: Ebisu
authors: Akio
authors: A
authors: Kato
authors: Hiroyuki
authors: H
authors: Azakami
mesh_terms: D017077:Culture Media, Conditioned / Q000378:metabolism; D016124:Eikenella corrodens / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D005663:Furans / Q000378:metabolism; D006714:Homoserine / Q000031:analogs & derivatives* / Q000378:metabolism; D006358:Hot Temperature; D007783:Lactones / Q000378:metabolism*; D014357:Trypsin / Q000378:metabolism
chemical_list: D017077:Culture Media, Conditioned; D005663:Furans; D007783:Lactones; C091088:N-octanoylhomoserine lactone; D006714:Homoserine; D014357:Trypsin; C113254:4-hydroxy-5-methyl-3(2H)-furanone