title: An epigenetic modifier enhances the production of anti-diabetic and anti-inflammatory sesquiterpenoids from Aspergillus sydowii.
abstract: The addition of a DNA methyltransferase inhibitor, 5-azacytidine, to Aspergillus sydowii fungus culture broth changed its secondary metabolites profile. Analysis of the culture broth extract led to the isolation of three new bisabolane-type sesquiterpenoids: (7S)-(+)-7-O-methylsydonol (1), (7S,11S)-(+)-12-hydroxysydonic acid (2) and 7-deoxy-7,14-didehydrosydonol (3), along with eight known compounds. The isolated compounds were evaluated for their anti-diabetic and anti-inflammatory activities. Among the isolates, (S)-(+)-sydonol (4) did not only potentiate insulin-stimulated glucose consumption but also prevented lipid accumulation in 3T3-L1 adipocytes. Additionally, (S)-(+)-sydonol (4) exhibited significant anti-inflammatory activity through inhibiting superoxide anion generation and elastase release by fMLP/CB-induced human neutrophils. This is the first report on isolating a secondary metabolite with anti-diabetic and anti-inflammatory activities from microorganisms.
authors: Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors: Yu-Ming
authors: YM
authors: Chung
authors: Chien-Kei
authors: CK
authors: Wei
authors: Da-Wei
authors: DW
authors: Chuang
authors: Mohamed
authors: M
authors: El-Shazly
authors: Chi-Ting
authors: CT
authors: Hsieh
authors: Teigo
authors: T
authors: Asai
authors: Yoshiteru
authors: Y
authors: Oshima
authors: Tusty-Jiuan
authors: TJ
authors: Hsieh
authors: Tsong-Long
authors: TL
authors: Hwang
authors: Yang-Chang
authors: YC
authors: Wu
authors: Fang-Rong
authors: FR
authors: Chang
mesh_terms: D041721:3T3-L1 Cells; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D001230:Aspergillus / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D001374:Azacitidine / Q000378:metabolism*; D015254:DNA Modification Methylases / Q000037:antagonists & inhibitors; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D007218:Industrial Microbiology; D051379:Mice; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D013481:Superoxides / Q000037:antagonists & inhibitors
chemical_list: D000893:Anti-Inflammatory Agents; D007004:Hypoglycemic Agents; D012717:Sesquiterpenes; D013481:Superoxides; D015254:DNA Modification Methylases; D005947:Glucose; D001374:Azacitidine