title: Collapsed abnormal pollen1 gene encoding the Arabinokinase-like protein is involved in pollen development in rice.
abstract: We isolated a pollen-defective mutant, collapsed abnormal pollen1 (cap1), from Tos17 insertional mutant lines of rice (Oryza sativa). The cap1 heterozygous plant produced equal numbers of normal and collapsed abnormal grains. The abnormal pollen grains lacked almost all cytoplasmic materials, nuclei, and intine cell walls and did not germinate. Genetic analysis of crosses revealed that the cap1 mutation did not affect female reproduction or vegetative growth. CAP1 encodes a protein consisting of 996 amino acids that showed high similarity to Arabidopsis (Arabidopsis thaliana) l-arabinokinase, which catalyzes the conversion of l-arabinose to l-arabinose 1-phosphate. A wild-type genomic DNA segment containing CAP1 restored mutants to normal pollen grains. During rice pollen development, CAP1 was preferentially expressed in anthers at the bicellular pollen stage, and the effects of the cap1 mutation were mainly detected at this stage. Based on the metabolic pathway of l-arabinose, cap1 pollen phenotype may have been caused by toxic accumulation of l-arabinose or by inhibition of cell wall metabolism due to the lack of UDP-l-arabinose derived from l-arabinose 1-phosphate. The expression pattern of CAP1 was very similar to that of another Arabidopsis homolog that showed 71% amino acid identity with CAP1. Our results suggested that CAP1 and related genes are critical for pollen development in both monocotyledonous and dicotyledonous plants.
authors: Department of Biological Production, Faculty of Bioresource Sciences, Akita Prefectural University, Akita 010-0195, Japan. uken@akita-pu.ac.jp
authors: Kenji
authors: K
authors: Ueda
authors: Fumiaki
authors: F
authors: Yoshimura
authors: Akio
authors: A
authors: Miyao
authors: Hirohiko
authors: H
authors: Hirochika
authors: Ken-Ichi
authors: K
authors: Nonomura
authors: Hiroetsu
authors: H
authors: Wabiko
mesh_terms: D000595:Amino Acid Sequence; D029681:Arabidopsis Proteins / Q000235:genetics; D001089:Arabinose / Q000378:metabolism; D003001:Cloning, Molecular; D035264:Flowers / Q000235:genetics; D018506:Gene Expression Regulation, Plant; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D009154:Mutation; D012275:Oryza / Q000235:genetics* / Q000254:growth & development; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011058:Pollen / Q000235:genetics* / Q000254:growth & development*; D017386:Sequence Homology, Amino Acid; D013403:Sugar Phosphates / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D010940:Plant Proteins; D013403:Sugar Phosphates; C104973:arabinose-1-phosphate; D001089:Arabinose; D017853:Phosphotransferases (Alcohol Group Acceptor); C111816:ARA1 protein, Arabidopsis
references: Plant Cell. 2004 Apr;16(4):1008-20
references: 15031413
references: Plant Cell. 2006 Nov;18(11):2999-3014
references: 17138695
references: Science. 2003 Jul 18;301(5631):376-9
references: 12869764
references: Plant Physiol. 2002 Dec;130(4):1645-56
references: 12481048
references: Nucleic Acids Res. 1995 Mar 25;23(6):1087-8
references: 7731798
references: PLoS One. 2007 Aug 08;2(8):e718
references: 17684564
references: Plant Mol Biol. 2010 Feb;72(3):265-77
references: 19888555
references: Plant Cell. 2011 Feb;23(2):534-49
references: 21285328
references: Plant Physiol. 1991 Aug;96(4):1255-60
references: 16668327
references: Plant Cell. 2003 Aug;15(8):1771-80
references: 12897251
references: J Exp Bot. 2009;60(5):1479-92
references: 19321648
references: Mol Genet Genomics. 2004 Mar;271(2):121-9
references: 14758540
references: Plant Cell. 2004;16 Suppl:S142-53
references: 15037731
references: Plant Physiol. 2013 Feb;161(2):663-75
references: 23263792
references: Gene. 1998 Mar 16;209(1-2):201-10
references: 9524266
references: Plant Cell Physiol. 2006 Nov;47(11):1457-72
references: 16990291
references: Plant Cell. 2003 Aug;15(8):1728-39
references: 12897248
references: Plant Physiol. 2003 Jun;132(2):640-52
references: 12805594
references: Nucleic Acids Res. 2012 Jan;40(Database issue):D302-5
references: 22053084
references: Plant Mol Biol. 2005 Apr;57(6):835-53
references: 15952069
references: BMC Plant Biol. 2011 May 09;11:78
references: 21554676
references: Nat Commun. 2013;4:1445
references: 23385589
references: Nucleic Acids Res. 2011 Jan;39(Database issue):D1141-8
references: 21045061
references: Mol Cells. 2011 Jun;31(6):523-30
references: 21499832
references: J Exp Bot. 2009;60(5):1465-78
references: 19213812
references: J Genet Genomics. 2011 Sep 20;38(9):379-90
references: 21930097
references: Plant Physiol. 2005 Jun;138(2):744-56
references: 15908605
references: Plant Mol Biol. 2005 Jul;58(5):721-37
references: 16158245
references: Plant Cell. 2011 Apr;23(4):1416-34
references: 21467577
references: Curr Opin Plant Biol. 2008 Jun;11(3):236-43
references: 18486535
references: Curr Biol. 2005 Dec 6;15(23):2107-11
references: 16332535
references: Mol Biol Evol. 1987 Jul;4(4):406-25
references: 3447015
references: Plant Cell. 2005 Mar;17(3):760-75
references: 15722468
references: Proc Natl Acad Sci U S A. 2013 Jan 2;110(1):76-81
references: 23256151
references: Proc Natl Acad Sci U S A. 2007 Sep 25;104(39):15566-71
references: 17878302
references: Plant J. 1994 Aug;6(2):271-82
references: 7920717
references: Nat Cell Biol. 2002 Sep;4(9):711-4
references: 12198497
references: Plant Cell Physiol. 2005 Jan;46(1):23-47
references: 15659435
references: Proc Natl Acad Sci U S A. 2010 Nov 23;107(47):20417-22
references: 21048083
references: Plant Cell. 2005 Oct;17(10):2705-22
references: 16141453
references: Plant Cell. 2006 Nov;18(11):3015-32
references: 17138699
references: Plant J. 2008 Jun;54(5):911-23
references: 18315544
references: Plant Cell. 2004 Jan;16(1):33-44
references: 14688295
references: Plant Physiol. 2008 Mar;146(3):1322-32
references: 18218968
references: Plant J. 2007 Sep;51(5):919-30
references: 17617177
references: Plant Mol Biol. 1999 Mar;39(5):1003-12
references: 10344205
references: Stain Technol. 1985 Sep;60(5):265-9
references: 2412314
references: Plant Cell Physiol. 2008 Oct;49(10):1407-16
references: 18755754
references: J Exp Bot. 2010 Aug;61(13):3639-46
references: 20603282
references: Nucleic Acids Res. 2006 Jan 1;34(Database issue):D741-4
references: 16381971
references: Dev Cell. 2012 Jun 12;22(6):1127-37
references: 22698279
references: Stain Technol. 1969 May;44(3):117-22
references: 4181665
references: Plant Cell. 2009 May;21(5):1453-72
references: 19454733
references: Plant Cell. 2007 Dec;19(12):3876-88
references: 18083909
references: Proc Natl Acad Sci U S A. 1996 Jul 23;93(15):7783-8
references: 8755553
references: Plant Cell. 2011 Feb;23(2):515-33
references: 21297036
references: Proc Natl Acad Sci U S A. 2010 Jan 26;107(4):1494-9
references: 20080642
references: Sex Plant Reprod. 2011 Jun;24(2):149-60
references: 21103996
references: Plant Cell. 2010 Mar;22(3):672-89
references: 20305120
references: Plant Physiol. 2006 Dec;142(4):1480-92
references: 17071651
references: Planta. 2006 Aug;224(3):520-32
references: 16557401
references: Nucleic Acids Res. 2012 Jan;40(Database issue):D290-301
references: 22127870
references: Plant Cell Physiol. 2008 Oct;49(10):1417-28
references: 18776202
references: Plant J. 2009 Jul;59(2):303-15
references: 19392701
references: Biosci Biotechnol Biochem. 2007 Mar;71(3):761-71
references: 17341835
references: Mol Cell Biol. 1991 Feb;11(2):620-31
references: 1846667
references: PLoS Genet. 2011 Jan 06;7(1):e1001265
references: 21253568
references: Plant Cell. 2007 Aug;19(8):2583-94
references: 17675402
references: J Cell Sci. 1989 Feb;92 ( Pt 2):217-29
references: 2476454
references: Plant Cell. 2007 Mar;19(3):847-61
references: 17400897
references: Arch Biochem Biophys. 1999 Dec 1;372(1):135-42
references: 10562426
references: Plant Cell. 2006 Jun;18(6):1412-25
references: 16698945
references: Plant Physiol. 2003 Oct;133(2):713-25
references: 14500793
references: Plant Physiol. 2007 Sep;145(1):3-4
references: 17823270
references: Plant Physiol. 2010 Sep;154(1):149-62
references: 20610705
references: Plant Cell. 2010 Jan;22(1):173-90
references: 20086189