title: Spectroscopic exploring the affinities, characteristics, and mode of binding interaction of curcumin with DNA.
abstract: Curcumin is a polyphenolic bioactive compound found in the spice turmeric endowed with diverse pharmacological and biological activities. In this study, fluorescence spectroscopy in combination with UV-Vis absorbance spectroscopy was employed to investigate the high affinity binding of curcumin to herring sperm DNA (hs-DNA). From the series of studies undertaken in the present program, for example, steady-state emission; absorption; the effect of denaturants; competition experiment; and anion (iodide) ion-induced fluorescence quenching; the mode of binding of curcumin into the DNA helix has been substantiated to be principally intercalative. Binding parameters calculating from Stern-Volmer method and Scatchard method showed that curcumin bind to hs-DNA with the binding affinities of the order 10(4 )L mol(-1). The effects of ionic strength, chemical denaturants, thermal denaturation and pH were studied to show the factors of the interaction, and provided further support for the intercalative binding mode. In addition, the methods and techniques used in the present work can also be exploited to study the interaction of curcumin with other biological, biomimicking assemblies and drug delivery vehicles, and so forth.
authors: Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, Department of Chemistry, Hubei Normal University, Huangshi, 435002, People's Republic of China.
authors: Xiao-Ling
authors: XL
authors: Li
authors: Yan-Jun
authors: YJ
authors: Hu
authors: Ran
authors: R
authors: Mi
authors: Xiao-Yun
authors: XY
authors: Li
authors: Pei-Qi
authors: PQ
authors: Li
authors: Yu
authors: Y
authors: Ouyang
mesh_terms: D000042:Absorption; D000818:Animals; D001667:Binding, Competitive; D003474:Curcumin / Q000737:chemistry* / Q000378:metabolism; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009994:Osmolar Concentration; D013050:Spectrometry, Fluorescence; D013057:Spectrum Analysis; D013816:Thermodynamics
chemical_list: D004247:DNA; D003474:Curcumin
references: J Cell Mol Med. 2010 Apr;14(4):970-81
references: 20569277
references: Biochemistry. 2012 Mar 6;51(9):1825-35
references: 22329404
references: J Fluoresc. 2011 Mar;21(2):475-85
references: 20936333
references: Langmuir. 2012 May 1;28(17):7063-70
references: 22512327
references: J Agric Food Chem. 2012 May 30;60(21):5373-9
references: 22506728
references: Biosens Bioelectron. 2012 Mar 15;33(1):222-7
references: 22305389
references: Spectrochim Acta A Mol Biomol Spectrosc. 2012 Feb;86:351-9
references: 22093523
references: J Fluoresc. 2011 Jul;21(4):1439-53
references: 21344224
references: Nucleic Acids Res. 1979 Aug 24;6(12):3879-90
references: 493128
references: J Phys Chem B. 2010 Feb 11;114(5):2044-50
references: 20088531
references: Mol Biol Rep. 2012 May;39(5):5495-501
references: 22187343
references: Nat Prod Rep. 2011 Nov;28(12):1937-55
references: 21979811
references: DNA Cell Biol. 2009 Apr;28(4):201-8
references: 19364279
references: Mol Pharm. 2011 Feb 7;8(1):126-32
references: 21133414
references: J Am Chem Soc. 2009 Apr 1;131(12):4490-8
references: 19256547
references: Biomacromolecules. 2011 Mar 14;12(3):797-803
references: 21309587
references: Biochim Biophys Acta. 2011 Apr;1810(4):485-96
references: 21281702
references: Anal Bioanal Chem. 2011 Apr;400(2):321-7
references: 21243339
references: J Inorg Biochem. 2012 Jan;106(1):1-9
references: 22105011
references: Anal Chem. 2011 Jun 15;83(12):4930-5
references: 21561110
references: J Phys Chem B. 2011 Oct 20;115(41):11938-49
references: 21899350
references: Int J Cancer. 2011 Jan 1;128(1):242-5
references: 20198612
references: J Agric Food Chem. 2011 Aug 24;59(16):8944-52
references: 21761894
references: J Agric Food Chem. 2012 Feb 22;60(7):1880-6
references: 22268806
references: Acc Chem Res. 2011 May 17;44(5):349-59
references: 21446672
references: Biomacromolecules. 2007 Mar;8(3):920-7
references: 17315924
references: Arch Biochem Biophys. 2012 Mar 1;519(1):46-58
references: 22286030
references: Biomacromolecules. 2011 Feb 14;12(2):511-7
references: 21192723
references: Inorg Chem. 2012 Feb 20;51(4):2046-57
references: 22288501
references: J Phys Chem B. 2012 May 3;116(17):5226-33
references: 22506549
references: J Photochem Photobiol B. 2012 Apr 2;109:58-67
references: 22386775
references: J Agric Food Chem. 2010 May 12;58(9):5257-62
references: 20402507
references: Phys Rev Lett. 2011 Jan 28;106(4):048101
references: 21405364
references: J Pharm Biomed Anal. 2012 Apr 7;63:40-6
references: 22349882
references: Anal Chem. 2010 Oct 1;82(19):8211-6
references: 20806886
references: Biomacromolecules. 2008 Oct;9(10):2712-8
references: 18729321
references: J Phys Chem B. 2008 Jul 31;112(30):9243-9
references: 18610959
references: J Photochem Photobiol B. 2012 Mar 1;108:53-61
references: 22245468
references: Curr Med Chem. 2010;17(3):190-7
references: 20214562
references: Biomacromolecules. 2012 Mar 12;13(3):873-80
references: 22316074
references: J Phys Chem B. 2011 Oct 27;115(42):12247-56
references: 21910473
references: Org Biomol Chem. 2011 Mar 7;9(5):1328-31
references: 21221451