title: Phylogeny and expression of the nucleocapsid gene of porcine epidemic diarrhoea virus.
abstract: Porcine epidemic diarrhoea virus (PEDV) is one of the important pathogens that may cause severe diarrhoea in piglets. In this study, the nucleocapsid (N) gene of a Chinese PEDV isolate designated HLJBY was cloned. The phylogeny of PEDV strains was investigated by constructing a phylogenetic tree based on the N protein sequences. The results indicate that there are two major groups of Chinese PEDVs, a Japanese PEDV group and a Korean PEDV group. High-level expression of the N protein was achieved in Escherichia coli. The immunoreactivity between PEDV particles or the bacterially expressed N protein and rabbit anti-PEDV serum was confirmed by immunofluorescence assays and Western blot. Both PEDV N protein and the polyclonal antibody generated in this study are valuable diagnostic reagents for PEDV surveillance.
authors: Northeast Agricultural University College of Veterinary Medicine 59 Mucai Street, Xiangfang District 150030 Harbin China.
authors: Wei
authors: W
authors: Yang
authors: Guangxing
authors: G
authors: Li
authors: Yudong
authors: Y
authors: Ren
authors: Siqingaowa
authors: S
authors: Suo
authors: Xiaofeng
authors: X
authors: Ren
mesh_terms: D000818:Animals; D018352:Coronavirus Infections; D008969:Molecular Sequence Data; D019251:Nucleocapsid; D010802:Phylogeny; D053485:Porcine epidemic diarrhea virus; D013553:Swine Diseases / Q000453:epidemiology