title: [Effect of tanshinone II A to B7H3 expression in human nasopharyngeal carcinoma CNE1 cell lines].
abstract: OBJECTIVE
  To explore the proliferation of human nasopharyngeal carcinoma CNE1 cell and B7H3 expiration after treating by Tanshinone II A (Tan II A).
  
  
  METHODS
  The CNE1 cell was cultured in vitro and treated by Tan II A with different concentrations; Cell proliferation was valuated by MTT; Apoptotic rate and cell cycle were investigated by flow cytometry; Bcl2 and B7H3 expiration were detected by direct immunofluorescence assay.
  
  
  RESULTS
  The CNE1 cell's proliferation has been significantly inhibited by Tan II A, the inhibition was in dose and time effect with Tan II A, with significant difference (P<0.01); IC50 value in 24, 48 and 72 hours was respective 12.5 micromol/L, 4.8 micromol/L and 3.0 micromol/L; Comparing with the control group, Tan II A group increased apoptosis rate (P<0.01); Cell cycle was blocked in G2/M phase; Bcl2 and B7H3 expression were more decreased in Tan II A group than in control group with significant difference (P<0.05);
  
  
  CONCLUSIONS
  Tan II A caused obviously growth inhibitory effect and induced apoptosis in CNE1 cells and the mechanisms might be related to down regulation of Bcl2 and B7H3.
authors: Department of Otorhinolaryngology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200062, China.
authors: Jian
authors: J
authors: Zhao
authors: Feng
authors: F
authors: Xu
authors: Jun
authors: J
authors: Wei
authors: Ying
authors: Y
authors: Li
authors: Wei
authors: W
authors: Shen
mesh_terms: D045784:Abietanes / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D060887:B7 Antigens / Q000378:metabolism*; D002277:Carcinoma; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism / Q000473:pathology; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism
chemical_list: D045784:Abietanes; D060887:B7 Antigens; C423237:CD276 protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; C021751:tanshinone