title: Senescence marker protein-30 (SMP30) deficiency impairs myocardium-induced dilation of coronary arterioles associated with reactive oxygen species.
abstract: Senescence marker protein-30 (SMP30) decreases with aging. Mice with SMP30 deficiency, a model of aging, have a short lifespan with increased oxidant stress. To elucidate SMP30's effect on coronary circulation derived from myocytes, we measured the changes in the diameter of isolated coronary arterioles in wild-type (WT) mice exposed to supernatant collected from isolated paced cardiac myocytes from SMP30 KO or WT mice. Pacing increased hydrogen peroxide in myocytes, and hydrogen peroxide was greater in SMP30 KO myocytes compared to WT myocytes. Antimycin enhanced and FCCP (oxidative phosphorylation uncoupler in mitochondria) decreased superoxide production in both groups. Addition of supernatant from stimulated myocytes, either SMP30 KO or WT, caused vasodilation. The degree of the vasodilation response to supernatant was smaller in SMP30 KO mice compared to WT mice. Administration of catalase to arterioles eliminated vasodilation in myocyte supernatant of WT mice and converted vasodilation to vasoconstriction in myocyte supernatant of SMP30 KO mice. This vasoconstriction was eliminated by olmesartan, an angiotensin II receptor antagonist. Thus, SMP30 deficiency combined with oxidant stress increases angiotensin and hydrogen peroxide release from cardiac myocytes. SMP30 plays an important role in the regulation of coronary vascular tone by myocardium.
authors: Department of Cardiology and Hematology, Fukushima Medical University, 1 Hikarigaoka, Fukushima 960-1295, Japan. sais@fmu.ac.jp.
authors: Hiroyuki
authors: H
authors: Mizukami
authors: Shu-Ichi
authors: S
authors: Saitoh
authors: Hirofumi
authors: H
authors: Machii
authors: Shinya
authors: S
authors: Yamada
authors: Yasuto
authors: Y
authors: Hoshino
authors: Tomofumi
authors: T
authors: Misaka
authors: Akihito
authors: A
authors: Ishigami
authors: Yasuchika
authors: Y
authors: Takeishi
mesh_terms: D000818:Animals; D000968:Antimycin A / Q000031:analogs & derivatives / Q000494:pharmacology; D001160:Arterioles / Q000502:physiology*; D001205:Ascorbic Acid / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000172:deficiency / Q000378:metabolism*; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / Q000494:pharmacology; D002374:Catalase / Q000378:metabolism; D002470:Cell Survival; D003331:Coronary Vessels / Q000502:physiology*; D004558:Electric Stimulation; D006861:Hydrogen Peroxide / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins / Q000172:deficiency / Q000378:metabolism*; D008297:Male; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009206:Myocardium / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000201:enzymology / Q000378:metabolism; D019255:NADPH Oxidases / Q000378:metabolism; D017382:Reactive Oxygen Species; D013194:Staining and Labeling; D013347:Subcellular Fractions / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D014661:Vasoconstriction; D014664:Vasodilation
chemical_list: D002135:Calcium-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; D017382:Reactive Oxygen Species; C513324:Rgn protein, mouse; D013481:Superoxides; C032456:antimycin; D002259:Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; D000968:Antimycin A; D006861:Hydrogen Peroxide; D002374:Catalase; D019255:NADPH Oxidases; D001205:Ascorbic Acid
references: Am J Physiol Heart Circ Physiol. 2007 Dec;293(6):H3720-5
references: 17965288
references: J Clin Invest. 1991 Jun;87(6):2056-66
references: 1645750
references: Physiol Rev. 1983 Jan;63(1):1-205
references: 6296890
references: FEBS Lett. 2011 Jul 21;585(14):2291-4
references: 21684279
references: Mech Ageing Dev. 2010 Nov-Dec;131(11-12):710-7
references: 20965209
references: Circulation. 2006 Jan 3;113(1):60-6
references: 16380548
references: Biosci Biotechnol Biochem. 2012;76(7):1367-71
references: 22785482
references: J Biol Chem. 2004 Nov 19;279(47):49064-73
references: 15317809
references: Arterioscler Thromb Vasc Biol. 2006 Dec;26(12):2614-21
references: 17023676
references: Biochim Biophys Acta. 1995 Sep 19;1263(3):249-52
references: 7548213
references: Exp Gerontol. 2004 Aug;39(8):1169-77
references: 15288691
references: Am J Physiol Heart Circ Physiol. 2008 Feb;294(2):H724-35
references: 18055514
references: Mech Ageing Dev. 1994 May;74(1-2):121-33
references: 7934203
references: Biochem Biophys Res Commun. 2009 Dec 18;390(3):394-8
references: 19733551
references: Circ Res. 1999 Oct 15;85(8):690-8
references: 10521242
references: Am J Physiol. 1989 Jan;256(1 Pt 2):H66-73
references: 2912199
references: Circ Res. 2002 Jun 14;90(11):1159-66
references: 12065318
references: Eur J Pharmacol. 2010 Oct 25;645(1-3):127-34
references: 20670619
references: Arch Pharm Res. 2008 Jul;31(7):872-7
references: 18704329
references: Am J Pathol. 2002 Oct;161(4):1273-81
references: 12368201
references: Nutrition. 2011 Apr;27(4):471-8
references: 20708381
references: Am J Pathol. 2004 Sep;165(3):1019-32
references: 15331425
references: Am J Respir Crit Care Med. 2006 Sep 1;174(5):530-7
references: 16728709
references: Mech Ageing Dev. 2006 May;127(5):451-7
references: 16500693
references: Eur J Pharmacol. 2006 Aug 21;544(1-3):97-103
references: 16842772
references: Am J Physiol Cell Physiol. 2004 Oct;287(4):C817-33
references: 15355853
references: FEBS Lett. 1995 Mar 13;361(1):22-4
references: 7890034
references: Endocrinology. 1998 May;139(5):2579-87
references: 9564874
references: Methods Enzymol. 1984;105:121-6
references: 6727660
references: Am J Physiol Heart Circ Physiol. 2009 Jan;296(1):H226-32
references: 19028798
references: Hypertension. 2006 Apr;47(4):727-34
references: 16505203