title: Mapping the telomere integrated genome of human herpesvirus 6A and 6B.
abstract: Human herpesvirus 6B (HHV-6B) is the causative agent of roseola infantum. HHV-6A and 6B can reactivate in immunosuppressed individuals and are linked with severe inflammatory response, organ rejection and central nervous system diseases. About 0.85% of the US and UK population carries an integrated HHV-6 genome in all nucleated cells through germline transmission. We have previously reported that the HHV-6A genome integrated in telomeres of patients suffering from neurological dysfunction and also in telomeres of tissue culture cells. We now report that HHV-6B also integrates in telomeres during latency. Detailed mapping of the integrated viral genomes demonstrates that a single HHV-6 genome integrates and telomere repeats join the left end of the integrated viral genome. When HEK-293 cells carrying integrated HHV-6A were exposed to the histone deacetylase inhibitor Trichostatin A, circularization and/or formation of concatamers were detected and this assay could be used to distinguish between lytic replication and latency.
authors: Department of Molecular Medicine, University of South Florida College of Medicine, Tampa, FL 33612, United States.
authors: Jesse H
authors: JH
authors: Arbuckle
authors: Shara N
authors: SN
authors: Pantry
authors: Maria M
authors: MM
authors: Medveczky
authors: Joshua
authors: J
authors: Prichett
authors: Kristin S
authors: KS
authors: Loomis
authors: Dharam
authors: D
authors: Ablashi
authors: Peter G
authors: PG
authors: Medveczky
mesh_terms: D002460:Cell Line; D002874:Chromosome Mapping; D002877:Chromosomes, Human / Q000821:virology; D004261:DNA Replication; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D016679:Genome, Viral; D057809:HEK293 Cells / Q000187:drug effects / Q000821:virology; D015654:Herpesvirus 6, Human / Q000235:genetics*; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology; D008297:Male; D019349:Roseolovirus Infections / Q000821:virology; D016615:Telomere / Q000821:virology*; D016662:Virus Integration; D017735:Virus Latency
chemical_list: D004279:DNA, Viral; D006877:Hydroxamic Acids; C012589:trichostatin A
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