title: Impact of methimazole treatment on magnesium concentration and lymphocytes activation in adolescents with Graves' disease.
abstract: The aim of this research was to assess plasma magnesium (Mg) concentration, the frequencies of activated T CD4+ and T CD8+ lymphocytes and B lymphocytes in adolescents with hyperthyroidism due to Graves' disease (GD), and to assess changes in the above-mentioned parameters during methimazole (MMI) treatment. The frequencies of activated T and B cells were measured by flow cytometry method and plasma Mg concentration was determined by spectrophotometry method in 60 adolescents at the time of GD diagnosis and after receiving the normalisation of the thyroid hormones levels. The control group consisted of 20 healthy volunteers. We observed lower plasma Mg concentration, and higher frequencies of activated T and B lymphocytes in the study group before the treatment in comparison with healthy controls, and with study group in MMI-induced euthyreosis (p < 0.01).Statistically significant negative correlations between the percentages of activated T CD3+, T CD4+, T CD8+ and B CD19+ lymphocytes, and plasma Mg concentration before the treatment were found (r < -0.335, p < 0.002). After the treatment no vital differences in plasma Mg concentration, and in percentages of activated cells between GD patients and controls were found, except CD8+CD25+ cells (p = 0.03). The present study demonstrates that both activated T and B cells might play an important role in the pathogenesis of GD, and activation is related to Mg plasma level. The use of MMI in treatment of hyperthyroidism due to GD leads to decrease the frequencies of activated lymphocytes and normalisation of Mg levels.
authors: Department of Pediatric Endocrinology and Diabetology, Medical University of Lublin, Lublin, Poland.
authors: Maria
authors: M
authors: Klatka
authors: Ewelina
authors: E
authors: Grywalska
authors: Malgorzata
authors: M
authors: Partyka
authors: Malgorzata
authors: M
authors: Charytanowicz
authors: Jacek
authors: J
authors: Rolinski
mesh_terms: D000293:Adolescent; D013956:Antithyroid Agents / Q000627:therapeutic use*; D006111:Graves Disease / Q000097:blood / Q000188:drug therapy*; D006801:Humans; D008213:Lymphocyte Activation; D008274:Magnesium / Q000097:blood*; D008713:Methimazole / Q000627:therapeutic use*
chemical_list: D013956:Antithyroid Agents; D008713:Methimazole; D008274:Magnesium
references: J Pediatr Endocrinol Metab. 2005 Feb;18(2):155-64
references: 15751604
references: Acta Med Croatica. 2002;56(1):21-6
references: 12455450
references: J Biol Chem. 1999 Mar 12;274(11):7059-66
references: 10066762
references: Mol Cell. 2006 Apr 7;22(1):5-13
references: 16600865
references: Clin Endocrinol (Oxf). 2007 Apr;66(4):508-12
references: 17371467
references: Biol Trace Elem Res. 2013 Apr;152(1):9-15
references: 23322284
references: Trends Cell Biol. 2000 Sep;10(9):369-77
references: 10932094
references: Horm Metab Res. 2012 Jun;44(6):482-7
references: 22473756
references: Physiol Plant. 2008 Aug;133(4):692-704
references: 18724409
references: J Clin Endocrinol Metab. 1996 Oct;81(10):3733-9
references: 8855831
references: Immunol Rev. 2006 Aug;212:149-62
references: 16903912
references: Circ Res. 2000 Feb 18;86(3):245-8
references: 10679471
references: Toxicol Lett. 2002 Mar 10;128(1-3):117-27
references: 11869823
references: Int Immunopharmacol. 2012 Sep;14(1):82-93
references: 22709475
references: Int Immunol. 1998 Dec;10(12):1969-80
references: 9885918
references: Biol Trace Elem Res. 2013 Mar;151(3):344-9
references: 23264034
references: Can J Anaesth. 2003 Aug-Sep;50(7):732-46
references: 12944451
references: Clin Endocrinol (Oxf). 2006 Feb;64(2):189-95
references: 16430719
references: Biometals. 2002 Sep;15(3):225-35
references: 12206389
references: Scand J Immunol. 2012 Oct;76(4):371-7
references: 22725135
references: Horm Res Paediatr. 2011;76(4):221-5
references: 21811047
references: Immunopharmacol Immunotoxicol. 1999 Aug;21(3):565-82
references: 10466080
references: Biol Trace Elem Res. 2012 Aug;148(2):165-9
references: 22351157
references: Clin Exp Rheumatol. 2005 Nov-Dec;23(6):769-77
references: 16396693
references: J Immunol. 2011 Apr 15;186(8):4734-43
references: 21398613
references: Biol Trace Elem Res. 2012 Dec;150(1-3):116-22
references: 23054869
references: Proc Natl Acad Sci U S A. 2012 May 8;109(19):7409-14
references: 22474373
references: J Endocrinol. 2007 Oct;195(1):145-55
references: 17911406
references: Endokrynol Pol. 2012;63(2):92-6
references: 22538746
references: Int Immunopharmacol. 2012 Jul;13(3):354-61
references: 22569344
references: J Pediatr Endocrinol Metab. 2012;25(9-10):863-7
references: 23426814
references: Int J Oncol. 2013 May;42(5):1786-92
references: 23546309
references: Clin Endocrinol (Oxf). 2013 Aug;79(2):267-74
references: 23170961
references: Genes Immun. 2004 May;5(3):203-8
references: 15029234
references: J Pediatr Endocrinol Metab. 2006 Sep;19(9):1095-111
references: 17128557
references: Autoimmunity. 2007 Feb;40(1):31-7
references: 17364495
references: Biol Trace Elem Res. 2012 Dec;150(1-3):42-8
references: 22895810
references: Nature. 2011 Jul 27;475(7357):471-6
references: 21796205
references: Biol Trace Elem Res. 2000 Feb;73(2):163-73
references: 11049208
references: Nature. 2011 Jul 27;475(7357):462-3
references: 21796201
references: J Clin Endocrinol Metab. 1996 Sep;81(9):3346-51
references: 8784095
references: Clin Endocrinol (Oxf). 2011 Jan;74(1):1-8
references: 20455896
references: Clin Immunol Immunopathol. 1998 May;87(2):168-75
references: 9614932
references: Orbit. 2009;28(4):251-5
references: 19839884
references: World J Surg. 2013 Feb;37(2):369-75
references: 23188529
references: J Inorg Biochem. 2009 Jan;103(1):77-86
references: 18954910
references: Magnes Res. 2000 Jun;13(2):139-46
references: 10907231
references: Biochim Biophys Acta. 2013 Jun;1833(6):1542-52
references: 23507197
references: J Biol Chem. 2006 Jun 23;281(25):16906-16913
references: 16627475
references: Scand J Immunol. 2012 Jul;76(1):62-9
references: 22486170
references: J Leukoc Biol. 2012 Nov;92(5):1103-9
references: 22811411
references: Clin Endocrinol (Oxf). 2004 Nov;61(5):589-94
references: 15521961
references: Endocr J. 1996 Aug;43(4):397-402
references: 8930527
references: Immunobiology. 2013 Aug;218(8):1034-40
references: 23434301
references: Scand J Immunol. 2011 Nov;74(5):496-501
references: 21815908