title: Ribosomal protein S1 functions as a termination factor in RNA synthesis by Qβ phage replicase.
abstract: S1 is the largest ribosomal protein, and is vitally important for the cell. S1 is also a subunit of Qβ replicase, the RNA-directed RNA polymerase of bacteriophage Qβ. In both protein and RNA syntheses, S1 is commonly believed to bind to a template RNA at the initiation step, and not to be involved in later events. Here, we show that in Qβ replicase-mediated RNA synthesis, S1 functions at the termination step by promoting release of the product strand in a single-stranded form. This function is fulfilled by the N-terminal fragment comprising the first two S1 domains. The results suggest that S1 might also have a role other than mRNA binding in the ribosome.
authors: Institute of Protein Research of the Russian Academy of Sciences, Pushchino, Moscow 142290, Russia.
authors: Nikita N
authors: NN
authors: Vasilyev
authors: Zarina S
authors: ZS
authors: Kutlubaeva
authors: Victor I
authors: VI
authors: Ugarov
authors: Helena V
authors: HV
authors: Chetverina
authors: Alexander B
authors: AB
authors: Chetverin
mesh_terms: D002021:Buffers; D004261:DNA Replication; D017434:Protein Structure, Tertiary; D011777:Q beta Replicase / Q000378:metabolism*; D012330:RNA, Double-Stranded / Q000378:metabolism; D012367:RNA, Viral / Q000096:biosynthesis*; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000378:metabolism; D013997:Time Factors; D061785:Transcription Initiation, Genetic; D061806:Transcription Termination, Genetic
chemical_list: D002021:Buffers; D012330:RNA, Double-Stranded; D012367:RNA, Viral; D012269:Ribosomal Proteins; C015494:ribosomal protein S1; D011777:Q beta Replicase
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