title: Influence of different cell storage/culture conditions on spontaneous proliferation and level of tyrosine kinase receptor inhibition in two feline injection-site sarcoma cell lines.
abstract: Optimizing cell culture conditions is important when studying cell proliferation and viability, particularly in response to cytotoxic compounds. Altered cell storage conditions can adversely impact proliferation and viability in mortal cell lines. However, little is known regarding the effects on immortal feline cell lines. In the present study, two feline injection-site sarcoma (ISS) cell lines were evaluated under standard culture conditions and three alternative storage/culture conditions for spontaneous proliferation rate and sensitivity to masitinib, a highly selective tyrosine kinase inhibitor with activity against primary and metastatic ISS cell lines. Cell viability was assessed by 7-aminoactinomycin D and cytology. Spontaneous proliferation did not significantly differ across the FBS concentrations (10% vs. 1%) for one cell line, however, with the other cell line spontaneous proliferation was significantly decreased in the 1% FBS 1-step technique, and the cold step technique at both 1% and 10% FBS. When normalized to untreated control cells, the IC50 values for masitinib were comparable across all culture techniques. Furthermore, apoptosis appeared to be the primary mechanism of this proliferation inhibition. Our preliminary findings suggest that select feline sarcoma cell lines cultured in 10% FBS yield comparable cytotoxicity data even when subjected to varying storage/culture conditions.
authors: Department of Biosciences and Diagnostic Imaging, College of Veterinary Medicine, University of Georgia, Athens, Georgia 30602, USA.
authors: Brittany R
authors: BR
authors: Feldhaeusser
authors: Michelle
authors: M
authors: Turek
authors: Jessica
authors: J
authors: Lawrence
authors: Karen
authors: K
authors: Cornell
authors: Robert M
authors: RM
authors: Gogal
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001549:Benzamides; D002415:Cats; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D010880:Piperidines; D011309:Preservation, Biological / Q000379:methods*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology*; D011725:Pyridines; D012509:Sarcoma / Q000378:metabolism* / Q000473:pathology; D013844:Thiazoles / Q000494:pharmacology*
chemical_list: D001549:Benzamides; D010880:Piperidines; D047428:Protein Kinase Inhibitors; D011725:Pyridines; D013844:Thiazoles; C526575:masitinib