title: Spinal cord GABA receptors modulate the exercise pressor reflex in decerebrate rats.
abstract: Neurotransmitters and neuromodulators released by contraction-activated skeletal muscle afferents into the dorsal horn of the spinal cord initiate the central component of the exercise pressor reflex (EPR). Whether γ-aminobutyric acid (GABA), a major inhibitory neurotransmitter within the mammalian central nervous system, is involved in the modulation of the EPR at the level of dorsal horn remains to be determined. We performed local microinjection of either the GABA(A) antagonist bicuculline or the GABA(B) antagonist CGP 52432 into the ipisilateral L4/L5 dorsal horns to investigate the effect of GABA receptor blockade on the pressor response to either static contraction induced by stimulation of the peripheral end of L4/L5 ventral roots, passive stretch, or hindlimb arterial injection of capsaicin (0.1 μg/0.2 ml) in decerebrate rats. Microinjection of either bicuculline (1 mM, 100 nl) or CGP 52432 (10 mM, 100 nl) into the L4/5 dorsal horns significantly increased the pressor and cardioaccelerator responses to all stimuli. Microinjection of either bicuculline or CGP 52432 into the L5 dorsal horn significantly increased the pressor and cardioaccelerator responses to direct microinjection of l-glutatmate (10 mM, 100 nl) into this spinal segment. The disinhibitory effect of both GABA receptor antagonists on the EPR was abolished by microinjection of the broad-spectrum glutamate receptor antagonist kynurenate (10 mM/100 nl). These data suggest that 1) GABA exerts a tonic inhibition of the EPR at the level of dorsal horn; and 2) that an interaction between glutamatergic and GABAergic inputs exist at the level of dorsal horn, contributing to spinal control of the EPR.
authors: Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, NE 68198-5850, USA.
authors: Han-Jun
authors: HJ
authors: Wang
authors: Wei
authors: W
authors: Wang
authors: Kaushik P
authors: KP
authors: Patel
authors: George J
authors: GJ
authors: Rozanski
authors: Irving H
authors: IH
authors: Zucker
mesh_terms: D000818:Animals; D001596:Benzylamines / Q000008:administration & dosage / Q000494:pharmacology; D001640:Bicuculline / Q000008:administration & dosage / Q000494:pharmacology; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology*; D003655:Decerebrate State / Q000503:physiopathology*; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D058787:GABA-A Receptor Antagonists / Q000008:administration & dosage / Q000494:pharmacology; D018698:Glutamic Acid / Q000494:pharmacology; D007736:Kynurenic Acid / Q000494:pharmacology; D008297:Male; D008845:Microinjections; D023421:Models, Animal; D010721:Phosphinic Acids / Q000008:administration & dosage / Q000494:pharmacology; D010805:Physical Conditioning, Animal / Q000502:physiology*; D011311:Pressoreceptors / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D018079:Receptors, GABA / Q000187:drug effects / Q000502:physiology*; D013116:Spinal Cord / Q000502:physiology*
chemical_list: D001596:Benzylamines; D018691:Excitatory Amino Acid Antagonists; D058787:GABA-A Receptor Antagonists; D010721:Phosphinic Acids; D018079:Receptors, GABA; C083008:CGP 52432; D018698:Glutamic Acid; D007736:Kynurenic Acid; D001640:Bicuculline
keywords: blood pressure; decerebration; exercise; neurotransmitter; sympathetic nerve activity
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