title: Characterization of salt-adapted secreted lignocellulolytic enzymes from the mangrove fungus Pestalotiopsis sp.
abstract: Fungi are important for biomass degradation processes in mangrove forests. Given the presence of sea water in these ecosystems, mangrove fungi are adapted to high salinity. Here we isolate Pestalotiopsis sp. NCi6, a halotolerant and lignocellulolytic mangrove fungus of the order Xylariales. We study its lignocellulolytic enzymes and analyse the effects of salinity on its secretomes. De novo transcriptome sequencing and assembly indicate that this fungus possesses of over 400 putative lignocellulolytic enzymes, including a large fraction involved in lignin degradation. Proteomic analyses of the secretomes suggest that the presence of salt modifies lignocellulolytic enzyme composition, with an increase in the secretion of xylanases and cellulases and a decrease in the production of oxidases. As a result, cellulose and hemicellulose hydrolysis is enhanced but lignin breakdown is reduced. This study highlights the adaptation to salt of mangrove fungi and their potential for biotechnological applications.
authors: INRA, UMR1163-Biotechnologie des Champignons Filamenteux, ESIL-Polytech, Aix-Marseille University, 13288 Marseille, France. yonathan.arfi@gmail.com
authors: Yonathan
authors: Y
authors: Arfi
authors: Didier
authors: D
authors: Chevret
authors: Bernard
authors: B
authors: Henrissat
authors: Jean-Guy
authors: JG
authors: Berrin
authors: Anthony
authors: A
authors: Levasseur
authors: Eric
authors: E
authors: Record
mesh_terms: D005656:Fungal Proteins / Q000378:metabolism*; D016681:Genome, Fungal / Q000235:genetics; D006026:Glycoside Hydrolases / Q000737:chemistry / Q000378:metabolism; D008031:Lignin / Q000378:metabolism*; D013058:Mass Spectrometry; D058977:Molecular Sequence Annotation; D010088:Oxidoreductases / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D043982:Rhizophoraceae / Q000382:microbiology*; D054712:Salinity; D055049:Salt Tolerance / Q000187:drug effects / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000494:pharmacology; D059467:Transcriptome / Q000235:genetics; D013098:Xylariales / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*
chemical_list: D005656:Fungal Proteins; D012333:RNA, Messenger; C036909:lignocellulose; D012965:Sodium Chloride; D008031:Lignin; D010088:Oxidoreductases; D006026:Glycoside Hydrolases
references: Mycologia. 2004 Mar-Apr;96(2):219-25
references: 21148848
references: Proc Natl Acad Sci U S A. 2011 Sep 13;108(37):15079-84
references: 21876164
references: Mycol Res. 2003 Feb;107(Pt 2):231-5
references: 12747335
references: BMC Genomics. 2012 Feb 02;13:57
references: 22300648
references: Appl Environ Microbiol. 2011 Sep;77(17):6076-84
references: 21764951
references: J Basic Microbiol. 2010 Feb;50(1):5-20
references: 20175122
references: Nature. 2005 Apr 21;434(7036):980-6
references: 15846337
references: Appl Environ Microbiol. 2012 Jul;78(14):4893-901
references: 22544251
references: BMC Genomics. 2010 Nov 16;11:635
references: 21080939
references: Mol Ecol. 2008 Apr;17(7):1636-47
references: 18266620
references: Biotechnol Bioeng. 2011 Jun;108(6):1229-45
references: 21337342
references: Appl Environ Microbiol. 2008 May;74(9):2709-16
references: 18310430
references: Appl Biochem Biotechnol. 2011 Dec;165(7-8):1754-69
references: 21947763
references: Microbiology (Reading). 1996 Feb;142 ( Pt 2):435-440
references: 8932715
references: FEMS Microbiol Ecol. 2012 Feb;79(2):433-44
references: 22092266
references: Mol Phylogenet Evol. 2010 Nov;57(2):528-35
references: 20692352
references: Appl Microbiol Biotechnol. 2006 Jan;69(5):573-9
references: 16021487
references: FEMS Microbiol Rev. 2005 Jan;29(1):3-23
references: 15652973