title: [Secretion of Th1-type cytokines and production of HBV-specific cytotoxic T lymphocytes in C57BL/6 mice induced by cytoplasmic transduction peptide-HBcAg18-27-Tapasin].
abstract: OBJECTIVE
  To observe whether the fusion protein of cytoplasmic transduction peptide (CTP)-HBcAg18-27;-Tapasin could induce Th1-type cytokine secretion and expression of hepatitis B virus (HBV)-specific cytotoxic T lymphocytes (CTL) in C57BL/6 mice.
  
  
  METHODS
  C57BL/6 mice were randomly divided into 4 groups, with 5 mice in each group. The mice of 4 groups were intramuscularly injected with the fusion protein CTP-HBcAg18-27;-Tapasin, CTP-HBcAg18-27;, HBcAg18-27;-Tapasin (50 μg) and normal saline, respectively. The levels of cytokines secreted by T lymphocytes were detected by ELISA and intracellular cytokine of proliferative T cells by flow cytometry. The proliferation of T lymphocytes was observed using CCK-8 assay.
  
  
  RESULTS
  CTP-HBcAg18-27;-Tapasin not only induced significantly T lymphocyte proliferation, but also increased the production of cytokines (IFN-γ, IL-2) compared with CTP-HBcAg18-27; or HBcAg18-27;-Tapasin alone or normal saline. Moreover, CTP-HBcAg18-27;-Tapasin fusion protein increased significantly the percentages of IFN-γ(+);CD8(+); T cells.
  
  
  CONCLUSIONS
  The CTP-HBcAg18-27;-Tapasin fusion protein can effectively stimulate the secretion of Th1-type cytokines, induce T lymphocyte proliferation and increase the expression of HBV-specific CTLs in C57BL/6 mice.
authors: Department of Infectious Diseases, ShangShanghai Jiaotong University, Shanghai, China. tangyuyan361113248@163.com
authors: Yuyan
authors: Y
authors: Tang
authors: Xiaohua
authors: X
authors: Chen
authors: Zhenghao
authors: Z
authors: Tang
authors: Guoqing
authors: G
authors: Zang
authors: Yongsheng
authors: Y
authors: Yu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D049109:Cell Proliferation; D016207:Cytokines / Q000378:metabolism*; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D005260:Female; D006512:Hepatitis B Core Antigens / Q000737:chemistry*; D006515:Hepatitis B virus / Q000276:immunology*; D007167:Immunotherapy; D026901:Membrane Transport Proteins / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D009842:Oligopeptides / Q000737:chemistry / Q000235:genetics; D010446:Peptide Fragments / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D013045:Species Specificity; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D018417:Th1 Cells / Q000276:immunology*
chemical_list: D016207:Cytokines; D000939:Epitopes; D006512:Hepatitis B Core Antigens; D026901:Membrane Transport Proteins; D009842:Oligopeptides; D010446:Peptide Fragments; D011993:Recombinant Fusion Proteins; C107762:tapasin