title: First detection in Europe of the metallo-β-lactamase IMP-15 in clinical strains of Pseudomonas putida and Pseudomonas aeruginosa.
abstract: In a prospective study (2009-2011) in healthcare institutions from the Canary Islands (Spain), 6 out of 298 carbapenem non-susceptible Pseudomonas aeruginosa isolates produced a metallo-β-lactamase: four IMP-15, two VIM-2 (including one IMP-15-positive isolate) and one VIM-1. Multilocus sequence typing identified the single VIM-1-producing isolate as clone ST111 and two IMP-15-producing isolates as ST606, but, strikingly, bacterial re-identification revealed that the other three isolates (producing IMP-15 and/or VIM-2) were actually Pseudomonas putida. Further retrospective analysis revealed a very high prevalence (close to 50%) of carbapenem resistance in this environmental species. Hence, we report the simultaneous emergence in hospitals on the Canary Islands of P. putida and P. aeruginosa strains producing IMP-15, a metallo-β-lactamase not previously detected in Europe, and suggest an underestimated role of P. putida as a nosocomial reservoir of worrying transferable resistance determinants.
authors: Departamento de Microbiología, Hospital Universitario de Gran Canaria Doctor Negrín, Las Palmas de Gran Canaria, Spain.
authors: R
authors: R
authors: Gilarranz
authors: C
authors: C
authors: Juan
authors: J
authors: J
authors: Castillo-Vera
authors: F J
authors: FJ
authors: Chamizo
authors: F
authors: F
authors: Artiles
authors: I
authors: I
authors: Álamo
authors: A
authors: A
authors: Oliver
mesh_terms: D015373:Bacterial Typing Techniques; D015780:Carbapenems / Q000627:therapeutic use; D003428:Cross Infection / Q000382:microbiology*; D006801:Humans; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing; D011552:Pseudomonas Infections / Q000188:drug therapy / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D016958:Pseudomonas putida / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D012189:Retrospective Studies; D013030:Spain; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D015780:Carbapenems; D001618:beta-Lactamases; C547882:beta-lactamase IMP-15, Pseudomonas aeruginosa
keywords: Class B carbapenemases; Pseudomonas putida; environmental reservoirs; metallo-β-lactamases; transferable resistance determinants