title: Purification of recombinant catalase-peroxidase HPI from E. coli and its application in enzymatic polymerization reactions.
abstract: In this paper, a recombinant catalase-peroxidase HPI from Escherichia coli was prepared, purified, and used in enzymatic polymerization reactions for the production of several oligomeric products. We tested the enzyme on four different substrates, chosen as representative of phenols and anilines: phenol, 3-methoxyphenol, catechol, and aniline. The polymerization reactions were followed by SEC-HPLC analysis, and except for aniline, all the other substrates were completely converted into one or more polymerization products. Results showed that reactions performed with phenol and 3-methoxyphenol allowed the isolation of some oligomers of different weight: a 27-monomeric unit oligomer and a 23-U oligomer are the heaviest ones. Experiments performed with catechol showed the formation of oligomers of 7 U in the reaction with HPI. HPI polymerization reactions performed with aniline allowed the identification of two different oligomers, one of 4 U and one of 10 U. All the substrates have been also used in reactions catalyzed by HRP in the same reaction conditions. Several products were common to the two enzymes. This work suggests the use of HPI as an alternative enzyme in peroxidatic reactions for the production of different oligomers from phenols and other compounds.
authors: Dept. Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, 20126, Milan, Italy, patrizia.digennaro@unimib.it.
authors: Patrizia
authors: P
authors: Di Gennaro
authors: Anna
authors: A
authors: Bargna
authors: Ferdinando
authors: F
authors: Bruno
authors: Guido
authors: G
authors: Sello
mesh_terms: D000814:Aniline Compounds / Q000378:metabolism; D001704:Biopolymers / Q000378:metabolism*; D002374:Catalase / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D010636:Phenols / Q000378:metabolism; D058105:Polymerization; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D000814:Aniline Compounds; D001704:Biopolymers; D029968:Escherichia coli Proteins; D010636:Phenols; D011994:Recombinant Proteins; D002374:Catalase; C051253:katG protein, E coli; C023650:aniline