title: Design and physicochemical characterization of poly(amidoamine) nanoparticles and the toxicological evaluation in human endothelial cells: applications to peptide delivery to the brain.
abstract: In this study, we investigated nanoparticles formulated by self-assembly of a biodegradable poly(amidoamine) (PAA) and a fluorescently labeled peptide, in their capacity to internalize in endothelial cells and deliver the peptide, with possible applications for brain drug delivery. The nanoparticles were characterized in terms of size, surface charge, and loading efficiency, and were applied on human cerebral microvascular endothelial cells (hCMEC/D3) and human umbilical vein endothelial cells (Huvec) cells. Cell-internalization and cytotoxicity experiments showed that the PAA-based nanocomplexes were essentially nontoxic, and the peptide was successfully internalized into cells. The results indicate that these PAAs have an excellent property as nontoxic carriers for intracellular protein and peptide delivery, and provide opportunities for novel applications in the delivery of peptides to endothelial cells of the brain.
authors: Department of Biomedical Chemistry, Faculty of Science and Technology, MIRA Institute for Biomedical Technology & Technical Medicine, University of Twente, 217, 7500, AE Enschede, the Netherlands.
authors: Grégory
authors: G
authors: Coué
authors: Christian
authors: C
authors: Freese
authors: Ronald E
authors: RE
authors: Unger
authors: C James
authors: CJ
authors: Kirkpatrick
authors: Karin E
authors: KE
authors: Pickl
authors: Frank M
authors: FM
authors: Sinner
authors: Johan F J
authors: JF
authors: Engbersen
mesh_terms: D001921:Brain / Q000378:metabolism; D002478:Cells, Cultured; D004337:Drug Carriers / Q000737:chemistry; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D055806:Microvessels / Q000166:cytology; D053758:Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D009842:Oligopeptides / Q000008:administration & dosage / Q000737:chemistry*; D011073:Polyamines / Q000737:chemistry* / Q000633:toxicity
chemical_list: D004337:Drug Carriers; D009842:Oligopeptides; C531249:Poly(amidoamine); D011073:Polyamines