title: mCSF1, a nucleus-encoded CRM protein required for the processing of many mitochondrial introns, is involved in the biogenesis of respiratory complexes I and IV in Arabidopsis.
abstract: The coding regions of many mitochondrial genes in plants are interrupted by intervening sequences that are classified as group II introns. Their splicing is essential for the expression of the genes they interrupt and hence for respiratory function, and is facilitated by various protein cofactors. Despite the importance of these cofactors, only a few of them have been characterized. CRS1-YhbY domain (CRM) is a recently recognized RNA-binding domain that is present in several characterized splicing factors in plant chloroplasts. The Arabidopsis genome encodes 16 CRM proteins, but these are largely uncharacterized. Here, we analyzed the intracellular location of one of these hypothetical proteins in Arabidopsis, mitochondrial CAF-like splicing factor 1 (mCSF1; At4 g31010), and analyzed the growth phenotypes and organellar activities associated with mcsf1 mutants in plants. Our data indicated that mCSF1 resides within mitochondria and its functions are essential during embryogenesis. Mutant plants with reduced mCSF1 displayed inhibited germination and retarded growth phenotypes that were tightly associated with reduced complex I and IV activities. Analogously to the functions of plastid-localized CRM proteins, analysis of the RNA profiles in wildtype and mcsf1 plants showed that mCSF1 acts in the splicing of many of the group II intron RNAs in Arabidopsis mitochondria.
authors: Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, 50250, Israel.|Department of Plant Sciences, The Hebrew University of Jerusalem, Rehovot, 76100, Israel.
authors: Michal
authors: M
authors: Zmudjak
authors: Australian Research Council (ARC) Centre of Excellence in Plant Energy Biology, University of Western Australia, 35 Stirling Highway, Crawley, 6009, WA, Australia.
authors: Catherine
authors: C
authors: Colas des Francs-Small
authors: Center for Plant Science Innovation, University of Nebraska, Lincoln, NE 68588-0660, USA.
authors: Ido
authors: I
authors: Keren
authors: Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, 50250, Israel.
authors: Felix
authors: F
authors: Shaya
authors: Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, 50250, Israel.
authors: Eduard
authors: E
authors: Belausov
authors: Australian Research Council (ARC) Centre of Excellence in Plant Energy Biology, University of Western Australia, 35 Stirling Highway, Crawley, 6009, WA, Australia.
authors: Ian
authors: I
authors: Small
authors: Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Center, Bet Dagan, 50250, Israel.
authors: Oren
authors: O
authors: Ostersetzer-Biran
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000196:embryology / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D019069:Cell Respiration; D042967:Electron Transport Complex I / Q000378:metabolism*; D003576:Electron Transport Complex IV / Q000378:metabolism*; D018525:Germination; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007438:Introns / Q000235:genetics*; D008928:Mitochondria / Q000378:metabolism*; D008969:Molecular Sequence Data; D010641:Phenotype; D018515:Plant Leaves / Q000254:growth & development / Q000378:metabolism; D021381:Protein Transport; D034622:RNA Interference; D012323:RNA Processing, Post-Transcriptional; D012326:RNA Splicing / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics; D012639:Seeds / Q000254:growth & development / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D029681:Arabidopsis Proteins; D018749:RNA, Plant; D049452:Green Fluorescent Proteins; D003576:Electron Transport Complex IV; D042967:Electron Transport Complex I
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