title: Long-Term Stimulation with Electroacupuncture at DU20 and ST36 Rescues Hippocampal Neuron through Attenuating Cerebral Blood Flow in Spontaneously Hypertensive Rats.
abstract: This study was designed to investigate the effect of long-term electroacupuncture at Baihui (DU20) and Zusanli (ST36) on cerebral microvessels and neurons in CA1 region of hippocampus in spontaneously hypertensive rats (SHR). A total of 45 male Wistar rats and 45 SHR were randomly grouped, with or without electroacupuncture (EA) at DU20 and ST36, once every other day for a period of 8 weeks. The mean arterial pressure (MAP) was measured once every 2 weeks. Cerebral blood flow (CBF) and the number of open microvessels in hippocampal CA1 region were detected by Laser Doppler and immunohistochemistry, respectively. Nissl staining and Western blotting were performed, respectively, to determine hippocampus morphology and proteins that were implicated in the concerning signaling pathways. The results showed that the MAP in SHR increased linearly over the observation period and was significantly reduced following electroacupuncture as compared with sham control SHR rats, while no difference was observed in Wistar rats between EA and sham control. The CBF, learning and memory capacity, and capillary rarefaction of SHR were improved by EA. The upregulation of angiotensin II type I receptor (AT1R), endothelin receptor (ETAR), and endothelin-1 (ET-1) in SHR rats was attenuated by electroacupuncture, suggesting an implication of AT1R, ETAR, and ET-1 pathway in the effect of EA.
authors: School of Acupuncture and Moxibustion, Beijing University of Chinese Medicine, Beijing 100029, China ; Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 101121, China ; Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing 100191, China.
authors: Gui-Hua
authors: GH
authors: Tian
authors: Kai
authors: K
authors: Sun
authors: Ping
authors: P
authors: Huang
authors: Chang-Man
authors: CM
authors: Zhou
authors: Hai-Jiang
authors: HJ
authors: Yao
authors: Ze-Jun
authors: ZJ
authors: Huo
authors: Hui-Feng
authors: HF
authors: Hao
authors: Lei
authors: L
authors: Yang
authors: Chun-Shui
authors: CS
authors: Pan
authors: Ke
authors: K
authors: He
authors: Jing-Yu
authors: JY
authors: Fan
authors: Zhi-Gang
authors: ZG
authors: Li
authors: Jing-Yan
authors: JY
authors: Han
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