title: Genetic diversity of early (1998) and recent (2010) avian influenza H9N2 virus strains isolated from poultry in Iran.
abstract: Infection with avian influenza H9N2 virus is widespread in the Asian poultry industry, resulting in great economic losses due to mortality and a severe decline in egg production. To obtain more-comprehensive genomic data from circulating H9N2 viruses in Iran, we sequenced the whole genomes of early (Ck/IR/ZMT-101/98) and recent (Ck/IR/EBGV-88/10) isolates of this virus in Iran. The M and NS genes of Ck/IR/EBGV-88/10 shared a high level of similarity with a highly pathogenic H7N3 virus isolated from Pakistan. The cleavage site within the HA protein of these viruses contained two different motifs, RSSR and KSSR, which are similar to those found in low-pathogenic viruses. The deduced amino acid sequence of the new isolate contained the mutation Q226L, which is a characteristic of human-type sialic acid influenza receptor binding. An analysis of the viral amino acid sequence of the M2 protein of the recent strain revealed a V27A mutation, which is associated with amantadine resistance in avian influenza virus. The present results emphasize the need for continuous surveillance of H9N2 viruses in poultry and the human population to obtain more information about the nature and evolution of future pandemic influenza viruses.
authors: Department of Poultry Diseases, Faculty of Veterinary Medicine, University of Tehran, P.O. Box 14155-6453, Tehran, Iran.
authors: Mohsen
authors: M
authors: Bashashati
authors: Mehdi
authors: M
authors: Vasfi Marandi
authors: Fereshteh
authors: F
authors: Sabouri
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D014644:Genetic Variation; D006388:Hemagglutinins / Q000235:genetics / Q000378:metabolism; D053127:Influenza A Virus, H9N2 Subtype / Q000235:genetics*; D005585:Influenza in Birds / Q000453:epidemiology / Q000821:virology*; D007492:Iran / Q000453:epidemiology; D009439:Neuraminidase / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D011200:Poultry; D013997:Time Factors
chemical_list: D006388:Hemagglutinins; D009439:Neuraminidase