title: Cytogenetic characterization of an N-butyl-N-(4-hydroxybutyl) nitrosamine-induced mouse papillary urothelial carcinoma.
abstract: Chemically-induced urinary bladder cancer in rodents has long been used as a reliable model to study the biopathology of urinary bladder neoplasia and to develop therapeutic strategies against human tumors. Knowledge of the genetic basis underlying carcinogenesis would greatly enhance usability and usefulness of this model for the purposes of comparative pathology. However, little is known about the cytogenetic characteristics of rodent urinary bladder tumors. Accordingly, pathological and negative control specimens were collected for cytogenetic evaluation, from an ongoing mouse urinary bladder N-butyl-N-(4-hydroxybutyl) nitrosamine-induced carcinogenesis study. Histopathological analysis characterized the pathological sample as a papillary urothelial carcinoma. Conventional cytogenetic analysis revealed the presence of 66.3 % tetraploid cells. Fluorescent in situ hybridization using chromosome paint probes allowed the detection of a reciprocal translocation involving chromosomes 4 and 14 (containing the murine homologues to human p16 and retinoblastoma tumor-suppressor genes) in 42 % of tetraploid cells. The control sample showed no histological or cytogenetic changes. CDKN2A and RB1 loss of heterozygosity is associated with human early and advanced urinary bladder cancer, respectively. Thus, the present data paves the way for further studies concerning the molecular mechanisms of urinary bladder carcinogenesis.
authors: Department of Veterinary Sciences, ECAV, University of Trás-os-Montes and Alto Douro, 5001-801, Vila Real, Portugal.
authors: Regina
authors: R
authors: Arantes-Rodrigues
authors: Rosário
authors: R
authors: Pinto-Leite
authors: Rui Gil
authors: RG
authors: da Costa
authors: Aura
authors: A
authors: Colaço
authors: Carlos
authors: C
authors: Lopes
authors: Paula
authors: P
authors: Oliveira
mesh_terms: D059786:Abnormal Karyotype; D000818:Animals; D002085:Butylhydroxybutylnitrosamine; D002291:Carcinoma, Papillary / Q000139:chemically induced / Q000235:genetics / Q000473:pathology*; D002871:Chromosome Banding; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009374:Neoplasms, Experimental / Q000139:chemically induced / Q000235:genetics / Q000473:pathology; D057891:Tetraploidy; D014407:Tumor Cells, Cultured; D001743:Urinary Bladder / Q000473:pathology*; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced / Q000235:genetics / Q000473:pathology*; D019459:Urothelium / Q000473:pathology*
chemical_list: D002085:Butylhydroxybutylnitrosamine
references: Int J Exp Pathol. 2007 Feb;88(1):39-46
references: 17244337
references: PLoS One. 2010 Nov 03;5(11):e13821
references: 21072204
references: J BUON. 2011 Oct-Dec;16(4):589-601
references: 22331708
references: Rev Urol. 2008 Winter;10(1):31-43
references: 18470273
references: Cancer Genet Cytogenet. 2000 Dec;123(2):109-13
references: 11156735
references: Food Chem Toxicol. 1995 Sep;33(9):747-55
references: 7557748
references: Oncogene. 2011 Apr 21;30(16):1880-91
references: 21170085
references: J Natl Cancer Inst. 1992 Aug 19;84(16):1251-6
references: 1640484
references: Cancer Lett. 1998 May 15;127(1-2):9-13
references: 9619852
references: FASEB J. 2009 May;23(5):1441-6
references: 19126594
references: In Vivo. 2012 Jul-Aug;26(4):727-39
references: 22773588
references: Clin Cancer Res. 2003 Jan;9(1):235-42
references: 12538475
references: Urol Oncol. 2012 Jul-Aug;30(4):533-40
references: 22742566
references: Oncogene. 2011 May 5;30(18):2087-97
references: 21297668
references: Somat Cell Mol Genet. 1989 Sep;15(5):461-4
references: 2781416
references: Carcinogenesis. 2006 Mar;27(3):361-73
references: 16352616
references: Oncogene. 1995 Aug 17;11(4):635-45
references: 7651726
references: Mutat Res. 2002 Nov 26;521(1-2):81-9
references: 12438006
references: J Natl Cancer Inst. 1989 Mar 1;81(5):361-7
references: 2915372
references: Clin Cancer Res. 2004 Nov 15;10(22):7607-12
references: 15569992
references: Cell Div. 2012 Mar 14;7(1):10
references: 22417103
references: Cell Chromosome. 2005 Aug 07;4:1
references: 16083510
references: Genes Dev. 2009 Mar 15;23(6):655-9
references: 19299556
references: Toxicol Pathol. 2002 Nov-Dec;30(6):663-71
references: 12512866
references: J Surg Res. 2007 Dec;143(2):422-7
references: 17612565