title: Liquid-phase RNA synthesis by using alkyl-chain-soluble support.
abstract: Recent progress in the RNA therapeutics has increased demand for the synthesis of large quantities of oligoribonucleotides. The assembly of RNA oligomers relies mainly on solid-phase approaches. These allow rapid product purification and the ability to drive a target reaction to completion through the use of excess reagents. Despite the known advantages of solid-phase synthesis, some issues in the process remain to be addressed, such as low and limited scale, reagent accessibility, and the use of a very large excess of reagents. Herein, we report a highly efficient and practical method of liquid-phase synthesis of RNA oligomers by using alkyl-chain-soluble support. We demonstrate the utility of the liquid-phase method through 21-mer RNA synthesis on a gram scale.
authors: Laboratory of Bio-organic Chemistry, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan. agro-2@cc.tuat.ac.jp
authors: Shokaku
authors: S
authors: Kim
authors: Masanori
authors: M
authors: Matsumoto
authors: Kazuhiro
authors: K
authors: Chiba
mesh_terms: D009943:Organophosphorus Compounds / Q000737:chemistry; D010084:Oxidation-Reduction; D012313:RNA / Q000138:chemical synthesis* / Q000737:chemistry; D013777:Tetrazoles / Q000737:chemistry
chemical_list: D009943:Organophosphorus Compounds; D013777:Tetrazoles; C434331:phosphoramidite; D012313:RNA