title: Screening and identification of novel B cell epitopes of Toxoplasma gondii SAG1.
abstract: BACKGROUND
  The identification of protein epitopes is useful for diagnostic purposes and for the development of peptide vaccines. In this study, the epitopes of Toxoplasma gondii SAG1 were identified using synthetic peptide techniques with the aid of bioinformatics.
  
  
  RESULTS
  Eleven peptides derived from T. gondii SAG1 were assessed by ELISA using pig sera from different time points after infection. Four (PS4, PS6, PS10 and PS11), out of the eleven peptides tested were recognized by all sera. Then, shorter peptides that were derived from PS4, PS6, PS10 and PS11 were predicted using bioinformatics and tested by experimentation. Four out of nine shorter peptides were identified successfully (amino acids 106-120, 166-180, 289-300 and 313-332).
  
  
  CONCLUSIONS
  We have precisely located the epitopes of T. gondii SAG1 using pig sera collected at different time points after infection. The identified epitopes may be useful for the further study of epitope-based vaccines and diagnostic reagents.
authors: State Key Laboratory of Veterinary Etiological Biology, Lanzhou, 730046, China. wangyh061001@163.com
authors: Yanhua
authors: Y
authors: Wang
authors: Guangxiang
authors: G
authors: Wang
authors: Delin
authors: D
authors: Zhang
authors: Hong
authors: H
authors: Yin
authors: Meng
authors: M
authors: Wang
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000235:genetics / Q000276:immunology*; D019295:Computational Biology; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D018604:Epitope Mapping; D018985:Epitopes, B-Lymphocyte / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology*; D017422:Sequence Analysis, DNA; D013552:Swine; D013997:Time Factors; D014124:Toxoplasmosis, Animal
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D016054:DNA, Protozoan; D018985:Epitopes, B-Lymphocyte; D015800:Protozoan Proteins; C047168:SAG1 antigen, Toxoplasma
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