title: The TT genotype of the MTHFR 677C > T polymorphism increases susceptibility to premature coronary artery disease in interaction with some of the traditional risk factors.
abstract: BACKGROUND
  The presence of several risk factors (genetic and non-genetic) has greater impact on the risk of premature coronary artery disease (CAD) than single risk factor.
  
  
  OBJECTIVE
  The aim of the study was to establish possible relations between genotypes and alleles of 677C>T polymorphism of MTHFR gene and some traditional risk factors e.g. elevated levels of lipid parameters and smoking in development of premature CAD.
  
  
  METHODS
  The groups comprised 152 patients with angiographically documented premature CAD (aged 42.9 +/- 5.5) and 121 age-matched blood donors (aged 42.3 +/- 6.5) were studied. The MTHFR 677C>T polymorphism was genotyped with Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method.
  
  
  RESULTS
  Patients with TT genotype who simultaneously smoked had increased risk of premature CAD compared to non-smoking cases with CC genotype (OR = 24.62). We also found that individuals with TT genotype and elevated LDL-cholesterol (LDL-chol.) level had significantly higher risk of CAD (OR = 9.92) than individuals with normal LDL-chol. level and CC genotype.
  
  
  CONCLUSIONS
  The present study shows that simultaneous presence of MTHFR TT genotype and smoking or elevated levels of LDL-chol. influences the risk of premature CAD. This findings give interesting contribution to gene-environment interaction problem that may have clinical implications in the future.
authors: Department of Applied Pharmacy, Medical University of Silesia, Sosnowiec, Poland. beatasarecka@poczta.onet.pl
authors: Beata
authors: B
authors: Sarecka-Hujar
authors: Iwona
authors: I
authors: Zak
authors: Jolanta
authors: J
authors: Krauze
mesh_terms: D000328:Adult; D003324:Coronary Artery Disease / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008055:Lipids / Q000097:blood; D008297:Male; D042965:Methylenetetrahydrofolate Reductase (NADPH2) / Q000235:genetics*; D011110:Polymorphism, Genetic; D012307:Risk Factors; D012907:Smoking
chemical_list: D008055:Lipids; D042965:Methylenetetrahydrofolate Reductase (NADPH2)