title: Diversity of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from inpatients of 30 hospitals in Orange County, California.
abstract: There is a need for a regional assessment of the frequency and diversity of MRSA to determine major circulating clones and the extent to which community and healthcare MRSA reservoirs have mixed. We conducted a prospective cohort study of inpatients in Orange County, California, systematically collecting clinical MRSA isolates from 30 hospitals, to assess MRSA diversity and distribution. All isolates were characterized by spa typing, with selective PFGE and MLST to relate spa types with major MRSA clones. We collected 2,246 MRSA isolates from hospital inpatients. This translated to 91/10,000 inpatients with MRSA and an Orange County population estimate of MRSA inpatient clinical cultures of 86/100,000 people. spa type genetic diversity was heterogeneous between hospitals, and relatively high overall (72%). USA300 (t008/ST8), USA100 (t002/ST5) and a previously reported USA100 variant (t242/ST5) were the dominant clones across all Orange County hospitals, representing 83% of isolates. Fifteen hospitals isolated more t008 (USA300) isolates than t002/242 (USA100) isolates, and 12 hospitals isolated more t242 isolates than t002 isolates. The majority of isolates were imported into hospitals. Community-based infection control strategies may still be helpful in stemming the influx of traditionally community-associated strains, particularly USA300, into the healthcare setting.
authors: Department of Infectious Disease Epidemiology, Imperial College London, London, United Kingdom.
authors: Lyndsey O
authors: LO
authors: Hudson
authors: Courtney R
authors: CR
authors: Murphy
authors: Brian G
authors: BG
authors: Spratt
authors: Mark C
authors: MC
authors: Enright
authors: Kristen
authors: K
authors: Elkins
authors: Christopher
authors: C
authors: Nguyen
authors: Leah
authors: L
authors: Terpstra
authors: Adrijana
authors: A
authors: Gombosev
authors: Diane
authors: D
authors: Kim
authors: Paul
authors: P
authors: Hannah
authors: Lydia
authors: L
authors: Mikhail
authors: Richard
authors: R
authors: Alexander
authors: Douglas F
authors: DF
authors: Moore
authors: Susan S
authors: SS
authors: Huang
mesh_terms: D002140:California / Q000453:epidemiology; D016000:Cluster Analysis; D003428:Cross Infection / Q000453:epidemiology*; D014644:Genetic Variation; D006761:Hospitals; D006801:Humans; D007297:Inpatients; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D058889:Molecular Typing; D011446:Prospective Studies; D013203:Staphylococcal Infections / Q000453:epidemiology*
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