title: [HMGB1/SREBP-1 mediated IFN-gamma-induced lipid deposition in mouse mesangial cells].
abstract: OBJECTIVE
  To explore the possible mechanism of lipid deposition induced by interferon-gamma (IFN-gamma).
  
  
  METHODS
  The mouse mesangial cells (MMC) were randomly divided into control group, stimulation group, stimulation + control vector group (sh-HMGB1) and stimulation+ specific sh-vector group (sh-SREBP-1). RT-PCR was used to detect the expression of HMGB1, SREBP-1 and fatty acid synthetase (FAS) mRNA; the protein expression was determined by Western blot.
  
  
  RESULTS
  The Oil Red O staining revealed that the mouse mesangial cells showed significant lipid droplet in IFN-gamma group. IFN-gamma up-regulated the expression of HMGB1, SREBP-1, FAS mRNA and protein time-dependently; Transfection of MMC with HMGB1 siRNA resulted in the suppression of SREBP-1, FAS protein levels induced by IFN-gamma, following with decrease of lipid deposition. Stimulation with HMGB1 markedly induced expression of SREBP-1, FAS expression and peaked at 8 h, decreased at 12 h compared with that at 8 h. Sh-SREBP-1 decreased the lipid deposition induced by HMGB1 in MMC.
  
  
  CONCLUSIONS
  IFN-gamma might induce lipid deposition in mouse mesangial cells partly by up-regulating the expression of HMGB1/SREBP-1/FAS.
authors: Department of Pathology, Hebei Medical University, Shijiazhuang 050017, China.
authors: Yu-Jun
authors: YJ
authors: Zhang
authors: Yan-Ning
authors: YN
authors: Chen
authors: Shu-Shen
authors: SS
authors: Zheng
authors: Qing-Juan
authors: QJ
authors: Liu
authors: Jun
authors: J
authors: Hao
authors: Yan
authors: Y
authors: Han
authors: Bao-Wei
authors: BW
authors: Yang
authors: Shu-Xia
authors: SX
authors: Liu
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D064429:Fatty Acid Synthases / Q000378:metabolism; D024243:HMGB1 Protein / Q000378:metabolism*; D007371:Interferon-gamma / Q000494:pharmacology*; D007684:Kidney Tubules / Q000166:cytology; D050356:Lipid Metabolism; D008297:Male; D050527:Mesangial Cells / Q000187:drug effects* / Q000378:metabolism*; D051379:Mice; D051780:Sterol Regulatory Element Binding Protein 1 / Q000378:metabolism*
chemical_list: D024243:HMGB1 Protein; C582808:HMGB1 protein, mouse; C497388:Srebf1 protein, mouse; D051780:Sterol Regulatory Element Binding Protein 1; D007371:Interferon-gamma; D064429:Fatty Acid Synthases