title: Bovine papillomavirus type 13 DNA in equine sarcoids.
abstract: Equine sarcoids are locally aggressive fibroblastic neoplasms considered to be the most common skin tumors of horses worldwide. Bovine papillomavirus types 1 and 2 have typically been associated with sarcoids in equids. Investigations aiming to identify papillomavirus strains, aside from bovine papillomaviruses 1 and 2, which might be associated with sarcoid lesions, have been lacking. The aim of this article is to report the identification of a third bovine papillomavirus type, bovine papillomavirus 13, associated with equine sarcoids. Six sarcoid lesions were collected from diverse anatomical sites on two horses from southern Brazil. To detect a broad spectrum of papillomavirus strains, eight degenerate primer pairs designed to detect conserved regions on the L1 and E1 genes were tested on the DNA samples. Direct sequencing was then performed on the obtained amplicons, and sequence identities were compared with sequences from all bovine papillomavirus types. The FAP59/FAP64, MY09/MY11, and AR-E1F2/AR-E1R4 sequences generated from the sarcoids were shown to present 99 to 100% identity with bovine papillomavirus 13, a new bovine papillomavirus type previously described in cattle. The results from this study suggest that there is a need to identify bovine papillomavirus type 13 and other papillomavirus strains that might be associated with sarcoids in diverse geographical areas; such investigations might establish the frequency of occurrence of this viral type in these common tumors of equids.
authors: Laboratory of Veterinary Microbiology, Veterinary Teaching Hospital, Universidade de Cuiaba, Cuiaba, Brazil.
authors: Michele
authors: M
authors: Lunardi
authors: Brígida Kussumoto
authors: BK
authors: de Alcântara
authors: Rodrigo Alejandro Arellano
authors: RA
authors: Otonel
authors: Wagner Borges
authors: WB
authors: Rodrigues
authors: Alice Fernandes
authors: AF
authors: Alfieri
authors: Amauri Alcindo
authors: AA
authors: Alfieri
mesh_terms: D000818:Animals; D001938:Brazil; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D006734:Horse Diseases / Q000821:virology*; D006736:Horses; D008969:Molecular Sequence Data; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D012507:Sarcoidosis / Q000662:veterinary* / Q000821:virology; D017422:Sequence Analysis, DNA
chemical_list: D004279:DNA, Viral
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