title: Effects of sodium ferulate on preventing steroid-induced femoral head osteonecrosis in rabbits.
abstract: The aim of this study is to investigate the effects and possible mechanisms of sodium ferulate (SF) on anti-apoptosis in steroid-induced femoral head osteonecrosis in rabbits. Japanese white rabbits were randomly divided into three groups (control group, treatment group, and model group), each with 24 rabbits. The model and treatment groups were first injected with an intravenous dose of horse serum, 10 ml/kg, three weeks later with an intravenous dose of 7.5 ml/kg, and two weeks later with an intramuscular dose of methylprednisolone, 45 mg/kg, three times in order to establish rabbit models of osteonecrosis. Concurrently, the treatment group was injected with intravenous doses of SF 20 mg/kg for two weeks, once per day. Three time points, Weeks 2, 4, and 8, were selected after modeling was completed. Osteonecrosis was verified by histopathology with haematoxylin-eosin (HE) staining. The apoptosis rate of osteonecrosis was observed by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. The apoptosis expressions of caspase-3 and Bcl-2 were analyzed by immunohistochemistry and Western blot. The rabbit models of osteonecrosis were successfully established and observed by HE staining. SF was effective in intervening in apoptosis and decreasing the apoptosis rate in femoral head necrosis by the immunohistochemistry and TUNEL assay (P<0.01). Western blot analysis indicated that there were statistical significances in the protein levels of caspase-3 and Bcl-2 (P<0.01). SF has a protective effect by reducing the incidence of early steroid-induced femoral head necrosis in rabbits, effectively intervening in apoptosis through decreasing caspase-3 expression and up-regulating Bcl-2 expression.
authors: Department of Orthopedics, the Second Affiliated Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an 710004, China.
authors: Lei
authors: L
authors: Tian
authors: Xiao-qian
authors: XQ
authors: Dang
authors: Chun-sheng
authors: CS
authors: Wang
authors: Pei
authors: P
authors: Yang
authors: Chen
authors: C
authors: Zhang
authors: Kun-zheng
authors: KZ
authors: Wang
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage; D017209:Apoptosis / Q000187:drug effects*; D003373:Coumaric Acids / Q000008:administration & dosage*; D004195:Disease Models, Animal; D004347:Drug Interactions; D005271:Femur Head Necrosis / Q000139:chemically induced* / Q000473:pathology / Q000517:prevention & control*; D006801:Humans; D008297:Male; D008775:Methylprednisolone; D011817:Rabbits; D016896:Treatment Outcome
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D003373:Coumaric Acids; C004999:ferulic acid; D008775:Methylprednisolone
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