title: Immunoextraction of testosterone and epitestosterone from human urine sample based on polyamidoamine modified silica.
abstract: Polyamidoamine dendrimer (PAMAM) is one of a number of dendritic polymers with precise molecular structure, highly geometric symmetry, and a large number of terminal groups. The polyamidoamine modified silica was synthesized with microwave assisted protocol. Anti-epitestosterone monoclonal antibodies were immobilized onto the PAMAM grafted silica and prepared an off-line immunoextraction column that applied in the extraction of testosterone and epitestosterone. The results showed that the affinity activity of the anti-epitestosterone monoclonal antibodies was remained at high level after immobilization. It was satisfactory to apply this new type of immunoextraction column to analyze testosterone and epitestosterone in spiked urine sample.
authors: College of Science, Beijing University of Chemical Technology, Beijing, PR China.
authors: Chunting
authors: C
authors: Zhang
authors: Ping
authors: P
authors: Su
authors: Siming
authors: S
authors: Wang
authors: Fangyu
authors: F
authors: Ding
authors: Yang
authors: Y
authors: Yang
authors: Yi
authors: Y
authors: Yang
mesh_terms: D004845:Epitestosterone / Q000302:isolation & purification* / Q000652:urine*; D006801:Humans; D008297:Male; D011073:Polyamines / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry*; D013739:Testosterone / Q000302:isolation & purification* / Q000652:urine*
chemical_list: C531249:Poly(amidoamine); D011073:Polyamines; D013739:Testosterone; D004845:Epitestosterone; D012822:Silicon Dioxide