title: Electrotriggered, spatioselective, quantitative gene delivery into a single cell nucleus by Au nanowire nanoinjector.
abstract: Delivery of bioactive materials into a cell is highly important in the study of cell biology and medical treatments. Ideal nanoinjectors should be able to deliver biomaterials with high spatial resolution while causing minimum cell damage. We developed a Au nanowire (NW) nanoinjector that has the thinnest diameter (100–150 nm) among the DNA delivering devices as well as optimum mechanical properties, minimizing cell damage. Well-defined (111) single-crystalline Au surface and high electric conductivity of a Au NW nanoinjector allow precisely timed and efficient electrochemical release of DNA molecules attached on a Au NW surface. Both linear DNA and plasmid DNA were delivered separately and showed successful expression. The Au NW nanoinjector would find important biomedical applications in the fields such as gene therapy, DNA vaccination, targeted drug delivery, and probe/control of cell signaling events.
authors: Department of Chemical and Biomolecular Engineering (BK21 Program), KAIST, Daejeon 305-701, Korea.
authors: Seung Min
authors: SM
authors: Yoo
authors: Mijeong
authors: M
authors: Kang
authors: Taejoon
authors: T
authors: Kang
authors: Dong Min
authors: DM
authors: Kim
authors: Sang Yup
authors: SY
authors: Lee
authors: Bongsoo
authors: B
authors: Kim
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism*; D004560:Electricity; D018014:Gene Transfer Techniques; D006046:Gold / Q000737:chemistry*; D053770:Nanowires
chemical_list: D006046:Gold