title: Juvenile hormone and its receptor, methoprene-tolerant, control the dynamics of mosquito gene expression.
abstract: Juvenile hormone III (JH) plays a key role in regulating the reproduction of female mosquitoes. Microarray time-course analysis revealed dynamic changes in gene expression during posteclosion (PE) development in the fat body of female Aedes aegypti. Hierarchical clustering identified three major gene clusters: 1,843 early-PE (EPE) genes maximally expressed at 6 h PE, 457 mid-PE (MPE) genes at 24 h PE, and 1,815 late-PE (LPE) genes at 66 h PE. The RNAi microarray screen for the JH receptor Methoprene-tolerant (Met) showed that 27% of EPE and 40% of MPE genes were up-regulated whereas 36% of LPE genes were down-regulated in the absence of this receptor. Met repression of EPE and MPE and activation of LPE genes were validated by an in vitro fat-body culture experiment using Met RNAi. Sequence motif analysis revealed the consensus for a 9-mer Met-binding motif, CACG(C)/TG(A)/G(T)/AG. Met-binding motif variants were overrepresented within the first 300 bases of the promoters of Met RNAi-down-regulated (LPE) genes but not in Met RNAi-up-regulated (EPE) genes. EMSAs using a combination of mutational and anti-Met antibody supershift analyses confirmed the binding properties of the Met consensus motif variants. There was a striking temporal separation of expression profiles among major functional gene groups, with carbohydrate, lipid, and xenobiotics metabolism belonging to the EPE and MPE clusters and transcription and translation to the LPE cluster. This study represents a significant advancement in the understanding of the regulation of gene expression by JH and its receptor Met during female mosquito reproduction.
authors: Departments of Entomology and Botany and Plant Sciences, Institute for Integrative Genome Biology, and Graduate Program in Genetics, Genomics and Bioinformatics, University of California, Riverside, CA 92521, USA.
authors: Zhen
authors: Z
authors: Zou
authors: Tusar T
authors: TT
authors: Saha
authors: Sourav
authors: S
authors: Roy
authors: Sang Woon
authors: SW
authors: Shin
authors: Tyler W H
authors: TW
authors: Backman
authors: Thomas
authors: T
authors: Girke
authors: Kevin P
authors: KP
authors: White
authors: Alexander S
authors: AS
authors: Raikhel
mesh_terms: D000330:Aedes / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D016000:Cluster Analysis; D005216:Fat Body / Q000254:growth & development / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D007605:Juvenile Hormones / Q000378:metabolism* / Q000494:pharmacology; D008726:Methoprene / Q000378:metabolism* / Q000494:pharmacology; D059372:Nucleotide Motifs / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D007605:Juvenile Hormones; D008726:Methoprene
keywords: hormone action; insect disease vector; insect-specific hormone
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