title: Molecular analysis of transcription start sites of separate copies of the suffix short retroelement in genome of Drosophila.
abstract: In order to study TSS in the suffix element, we used total RNA isolated from ovaries of Drosophila melanogaster. Using a 5'-RACE System (Invitrogen) and 454 sequencing, we found the full-length suffix sense transcripts. However, most 5'-RACE reads (>70%) correspond to 5'-truncated transcripts lacking the first 33-39 nucleotides. The data may indicate that these RNAs are products of the RNAi-related silencing mech-anism producing small RNAs that are larger than piRNAs. The full-length suffix transcripts could arise either from the F element or from active separate copies of suffix.
authors: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, str. Vavilov 32, Moscow, 119991 Russia.
authors: O S
authors: OS
authors: Zenina
authors: D M
authors: DM
authors: Fedoseeva
authors: O V
authors: OV
authors: Kretova
authors: N A
authors: NA
authors: Tchurikov
mesh_terms: D000818:Animals; D001483:Base Sequence; D004331:Drosophila melanogaster / Q000235:genetics*; D005260:Female; D049750:Genome, Insect / Q000235:genetics*; D008969:Molecular Sequence Data; D010053:Ovary / Q000378:metabolism; D018626:Retroelements / Q000235:genetics*; D024363:Transcription Initiation Site
chemical_list: D018626:Retroelements
references: PLoS One. 2011;6(7):e21882
references: 21779345
references: EMBO J. 1986 Sep;5(9):2341-2347
references: 16453705
references: Genetika. 2002 Aug;38(8):1090-6
references: 12244693
references: Cell. 1982 Feb;28(2):365-73
references: 6800659
references: PLoS One. 2007 May 30;2(5):e476
references: 17534426
references: Genetika. 2003 Feb;39(2):300-4
references: 12669428
references: Proc Natl Acad Sci U S A. 1987 Aug;84(16):5843-7
references: 2441397
references: Dokl Biochem Biophys. 2005 Jul-Aug;403:306-9
references: 16229149