title: Kif3a guides microtubular dynamics, migration and lumen formation of MDCK cells.
abstract: The microtubular motor Kinesin-2 and its subunit Kif3a are essential for the formation of primary cilia, an organelle implicated in a wide spectrum of developmental abnormalities. Outside cilia, Kinesin-2 mediated transport has been implicated in vesicle and N-cadherin transport, but it is unknown if and how extraciliary Kif3a affects basic cellular functions such as migration or the formation of multicellular structures. Here we show that tetracycline inducible depletion of Kif3a in MDCK cells slows epithelial cell migration. Microtubules at the leading edge of Kif3a depleted cells failed to grow perpendicularly into the leading edge and microtubular dynamics were dampened in Kif3a depleted cells. Loss of Kif3a retarded lateral membrane specification and completely prevented the formation of three-dimensional spheres in collagen. These data uncover that Kif3a regulates the microtubular cytoskeleton in the cell periphery and imply that extra-ciliary Kif3a has an unexpected function in morphogenesis.
authors: Department of Nephrology, University Hospital, Freiburg, Germany.
authors: Christopher
authors: C
authors: Boehlke
authors: Fruzsina
authors: F
authors: Kotsis
authors: Bjoern
authors: B
authors: Buchholz
authors: Christian
authors: C
authors: Powelske
authors: Kai-Uwe
authors: KU
authors: Eckardt
authors: Gerd
authors: G
authors: Walz
authors: Roland
authors: R
authors: Nitschke
authors: E Wolfgang
authors: EW
authors: Kuehn
mesh_terms: D000818:Animals; D002465:Cell Movement; D002923:Cilia / Q000502:physiology; D004285:Dogs; D016547:Kinesins / Q000502:physiology*; D061985:Madin Darby Canine Kidney Cells; D008870:Microtubules / Q000378:metabolism*; D009024:Morphogenesis; D055503:Protein Multimerization; D011499:Protein Processing, Post-Translational; D018874:Spheroids, Cellular / Q000166:cytology / Q000378:metabolism; D019108:Tight Junctions / Q000378:metabolism
chemical_list: D016547:Kinesins
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