title: Molecular alterations of EGFR in small intestinal adenocarcinoma.
abstract: OBJECTIVE
  Molecular testing for epidermal growth factor receptor (EGFR) mutations has recently become a standard practice for the management of patients with non-squamous none small cell lung cancer. Primary small intestine adenocarcinoma (SIA) is an uncommon malignancy, and EGFR mutation in the cancer has not been well characterized due to its rarity.
  
  
  METHODS
  A micro-tissue array with 53 SIAs and 24 surgically resected primary non-ampullary SIAs were studied. EGFR mutations were analyzed by DNA sequencing in 24 cases with formalin-fixed paraffin-embedded blocks. All 77 cases were examined by immunohistochemistry (IHC) using antibodies specific for the EGFR E746-A750 deletion in exon 19 (DEL), L858R point mutation in exon 21 (L858R), and total EGFR. EGFR amplifications were detected by fluorescence in situ hybridization.
  
  
  RESULTS
  A positive reaction of DEL-specific, L858R-specific, and total EGFR antibodies was detected in seven (9.1%), 5 (6.5%) and 35 (45.5%) of 77 SIAs by IHC, respectively. Positive reaction of the three antibodies was not significantly correlated with patient's age, gender, differentiation, and stage. EGFR gene amplification was assayed in 77 SIAs in micro-tissue array. Of 24 SIA samples that had DNA sequencing, two (8.3%) harbored exon 19 deletion and one (4.2%) harbored L858R point mutation. Only one case with EGFR amplification and two cases with polysomy were shown.
  
  
  CONCLUSIONS
  Our findings suggested that mutations and amplification in EGFR genes are minor events, and most of SIAs may be unsuitable to EGFR-TKIs treatment.
authors: Department of Pathology, School of Basic Medical Science, Wuhan University, 185 Donghu Road, Wuchang District, 430071, Wuhan, China.
authors: Yan
authors: Y
authors: Wang
authors: Cong-Qing
authors: CQ
authors: Jiang
authors: Jing
authors: J
authors: Guan
authors: Gui-Fang
authors: GF
authors: Yang
authors: Jun-Qiu
authors: JQ
authors: Yue
authors: Hong-Lei
authors: HL
authors: Chen
authors: Jing-Ling
authors: JL
authors: Xue
authors: Zhi-Gao
authors: ZG
authors: Xu
authors: Qun
authors: Q
authors: Qian
authors: Li-Fang
authors: LF
authors: Fan
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D000328:Adult; D000368:Aged; D000912:Antibodies, Neoplasm / Q000276:immunology; D004252:DNA Mutational Analysis; D066246:ErbB Receptors / Q000235:genetics*; D005260:Female; D005784:Gene Amplification; D006801:Humans; D007150:Immunohistochemistry; D007414:Intestinal Neoplasms / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D007421:Intestine, Small / Q000473:pathology*; D008297:Male; D008875:Middle Aged
chemical_list: D000912:Antibodies, Neoplasm; D066246:ErbB Receptors
references: Eur J Cancer. 2001 Sep;37 Suppl 4:S9-15
references: 11597399
references: Mod Pathol. 2008 May;21 Suppl 2:S16-22
references: 18437168
references: CA Cancer J Clin. 2009 Jul-Aug;59(4):225-49
references: 19474385
references: J Thorac Oncol. 2008 Feb;3(2):111-6
references: 18303429
references: Proc Natl Acad Sci U S A. 2004 Sep 7;101(36):13306-11
references: 15329413
references: J Thorac Oncol. 2010 Oct;5(10):1551-8
references: 20697298
references: Lung Cancer. 2011 Oct;74(1):35-40
references: 21444121
references: Clin Cancer Res. 2009 May 1;15(9):3023-8
references: 19366827
references: Br J Cancer. 2010 Jan 5;102(1):144-50
references: 19935793
references: Differentiation. 2007 Nov;75(9):770-87
references: 17999740
references: N Engl J Med. 2004 May 20;350(21):2129-39
references: 15118073
references: Clin Cancer Res. 2010 Jun 15;16(12):3163-70
references: 20423982
references: J Mol Diagn. 2005 Aug;7(3):396-403
references: 16049312
references: Cancer. 1984 Jan 1;53(1):23-5
references: 6690001
references: Am J Gastroenterol. 2005 Mar;100(3):703-10
references: 15743371
references: Mod Pathol. 2005 Oct;18(10):1350-6
references: 15832190
references: Clin Cancer Res. 2005 Feb 15;11(4):1368-71
references: 15746034
references: Cancer. 2001 Sep 1;92(5):1331-46
references: 11571750
references: J Mol Diagn. 2010 Mar;12(2):169-76
references: 20093391
references: J Transl Med. 2010 Dec 18;8:135
references: 21167064
references: Zhonghua Bing Li Xue Za Zhi. 2011 Oct;40(10):655-9
references: 22321541
references: Clin Cancer Res. 2006 Feb 1;12(3 Pt 1):839-44
references: 16467097
references: Cancer. 2004 Aug 1;101(3):518-26
references: 15274064
references: Diagn Mol Pathol. 2006 Jun;15(2):101-8
references: 16778591
references: BMC Cancer. 2011 May 27;11:205
references: 21619586
references: Gene. 2006 Jan 17;366(1):2-16
references: 16377102
references: Cancer Res. 2006 Apr 15;66(8):3992-5
references: 16618717
references: Cancer. 1999 Dec 15;86(12):2693-706
references: 10594865
references: J Clin Oncol. 2005 Apr 10;23(11):2513-20
references: 15738541
references: Clin Oncol (R Coll Radiol). 2007 Mar;19(2):143-9
references: 17355111
references: Science. 2004 Jun 4;304(5676):1497-500
references: 15118125
references: Lung Cancer. 2005 Dec;50(3):375-84
references: 16199108
references: Lung Cancer. 2011 Jul;73(1):45-50
references: 21129809
references: Am J Clin Pathol. 2006 Oct;126(4):608-15
references: 16938658
references: Clin Cancer Res. 2010 Jul 1;16(13):3349-55
references: 20570926