title: NMR (¹H) analysis of crude extracts detects light stress in Beta vulgaris and Spinacia oleracea leaves.
abstract: In highlight stress conditions, the mechanism of non-photochemical quenching (NPQ) of chlorophyll fluorescence is triggered at the chloroplast level. This process allows thermal quenching of the excessive excitation energy and it is strictly related to the efficiency of the xanthophyll cycle. Nowadays, the utilization of the nuclear magnetic resonance (NMR) spectroscopy provides a powerful complementary way for the identification and quantitative analysis of plant metabolites either in vivo or in tissue extracts. Seeing that the oxidative damage caused by light stress in plants and the consequent involvement of pigments are widely studied, NMR spectroscopy can be utilized to compare crude leaf extract at different levels of light stress, allowing an analysis of these compounds. In this paper, the identification of possible relationships between light stress and ¹H NMR signal variations is discussed. The analysis of the ¹H NMR (1D) spectra of two agronomic species (Spinacia oleracea and Beta vulgaris) exposed to different light intensities is presented. In particular, change in carotenoids and xanthophylls signals are analyzed.
authors: Department of Biology, University of Naples Federico II, Italy. cguadagn@uwyo.edu
authors: Carmela Rosaria
authors: CR
authors: Guadagno
authors: Marina
authors: M
authors: Della Greca
authors: Amalia
authors: A
authors: Virzo De Santo
authors: Nicola
authors: N
authors: D'Ambrosio
mesh_terms: D027461:Beta vulgaris / Q000502:physiology*; D002338:Carotenoids / Q000032:analysis / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D045424:Complex Mixtures / Q000032:analysis; D018556:Crops, Agricultural; D008027:Light; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D010936:Plant Extracts / Q000032:analysis; D018515:Plant Leaves / Q000502:physiology*; D018724:Spinacia oleracea / Q000502:physiology*; D013312:Stress, Physiological; D024341:Xanthophylls / Q000032:analysis / Q000378:metabolism
chemical_list: D045424:Complex Mixtures; D010936:Plant Extracts; D024341:Xanthophylls; D002734:Chlorophyll; D002338:Carotenoids; C005613:violaxanthin
references: Photosynth Res. 1986 Jan;10(3):303-8
references: 24435377
references: Plant Physiol. 1980 Feb;65(2):254-9
references: 16661170
references: J Agric Food Chem. 2006 Aug 9;54(16):6094-100
references: 16881722
references: J Exp Bot. 2005 Jan;56(410):255-65
references: 15520026
references: Biol Rev Camb Philos Soc. 2005 Feb;80(1):27-43
references: 15727037
references: Plant Physiol. 1975 Jun;55(6):1048-53
references: 16659207
references: Plant Physiol. 2001 Apr;125(4):1558-66
references: 11299337
references: Magn Reson Chem. 2005 Aug;43(8):625-38
references: 15986496
references: J Agric Food Chem. 2008 Jan 23;56(2):314-20
references: 18081249
references: Trends Biochem Sci. 1992 Feb;17(2):61-6
references: 1566330
references: J Exp Bot. 2005 Jan;56(411):375-82
references: 15611143
references: Anal Chem. 2007 Oct 15;79(20):7611-27
references: 17854156
references: Nature. 2005 Jul 7;436(7047):134-7
references: 16001075
references: Anal Biochem. 1993 Oct;214(1):238-44
references: 8250229
references: J Agric Food Chem. 2005 Feb 9;53(3):671-7
references: 15686418
references: Annu Rev Plant Physiol Plant Mol Biol. 2001 Jun;52:499-526
references: 11337407
references: Photosynth Res. 1990 Sep;25(3):147-50
references: 24420345
references: Annu Rev Plant Physiol Plant Mol Biol. 1996 Jun;47:655-684
references: 15012304
references: Annu Rev Plant Physiol Plant Mol Biol. 1999 Jun;50:333-359
references: 15012213