title: The role of vascular endothelial growth factor and matrix metalloproteinases in canine lymphoma: in vivo and in vitro study.
abstract: BACKGROUND
  Canine lymphoma represents the most frequent haematopoietic cancer and it shares some similarities with human non-Hodgkin lymphoma. Matrix metalloproteinases (MMPs) and vascular endothelial growth factor (VEGF) play a coordinated role during invasion and proliferation of malignant cells; however, little is known about their role in canine haematologic malignancies. The aim of this study was to investigate the mRNA and protein expression of VEGF and the most relevant MMPs in canine lymphoma. Lymph node aspirates from 26 B-cell and 21 T-cell lymphomas were collected. The protein expression levels of MMP-9, MMP-2 and VEGF-A were evaluated by immunocytochemistry, and the mRNA levels of MMP-2, MMP-9, MT1-MMP, TIMP-1, TIMP-2, RECK, VEGF-A and VEGF-164 were measured using quantitative RT-PCR.
  
  
  RESULTS
  MT1-MMP, TIMP-1 and RECK mRNA levels were significantly higher in T-cell lymphomas than in B-cell lymphomas. Higher mRNA and protein levels of MMP-9 and VEGF-A were observed in T-cell lymphomas than in B-cell lymphomas and healthy control lymph nodes. A positive correlation was found between MMP-9 and VEGF-A in T-cell lymphomas. Moreover, MMP-9, MT1-MMP, TIMP-1 and VEGF-A were expressed at the highest levels in high-grade T-cell lymphomas.
  
  
  CONCLUSIONS
  This study provides new information on the expression of different MMPs and VEGF in canine lymphoma, suggesting a possible correlation between different MMPs and VEGF, immunophenotype and prognosis.
authors: Department of Comparative Biomedicine and Food Science, University of Padova, Viale dell'Università 16, Agripolis Legnaro, PD, Italy.
authors: Arianna
authors: A
authors: Aricò
authors: Mery
authors: M
authors: Giantin
authors: Maria Elena
authors: ME
authors: Gelain
authors: Fulvio
authors: F
authors: Riondato
authors: Stefano
authors: S
authors: Comazzi
authors: Barbara C
authors: BC
authors: Rütgen
authors: Sabine E
authors: SE
authors: Essler
authors: Mauro
authors: M
authors: Dacasto
authors: Massimo
authors: M
authors: Castagnaro
authors: Luca
authors: L
authors: Aresu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004283:Dog Diseases / Q000378:metabolism / Q000503:physiopathology*; D004285:Dogs; D058851:GPI-Linked Proteins / Q000378:metabolism / Q000502:physiology; D008198:Lymph Nodes / Q000378:metabolism / Q000503:physiopathology; D008223:Lymphoma / Q000378:metabolism / Q000503:physiopathology / Q000662:veterinary*; D016393:Lymphoma, B-Cell / Q000378:metabolism / Q000503:physiopathology / Q000662:veterinary; D016399:Lymphoma, T-Cell / Q000378:metabolism / Q000503:physiopathology / Q000662:veterinary; D053511:Matrix Metalloproteinase 14 / Q000378:metabolism / Q000502:physiology; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism / Q000502:physiology; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism / Q000502:physiology; D020782:Matrix Metalloproteinases / Q000378:metabolism / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism / Q000502:physiology; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism / Q000502:physiology*
chemical_list: D058851:GPI-Linked Proteins; D019715:Tissue Inhibitor of Metalloproteinase-1; D042461:Vascular Endothelial Growth Factor A; D020782:Matrix Metalloproteinases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D053511:Matrix Metalloproteinase 14
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