title: Phage display antibodies for diagnostic applications.
abstract: With major developments in molecular biology, numerous display technologies have been successfully introduced for recombinant antibody production. Even so, phage display still remains the gold standard for recombinant antibody production. Its success is mainly attributed to the robust nature of phage particles allowing for automation and adaptation to modifications. The generation of monospecific binders provides a vital tool for diagnostics at a lower cost and higher efficiency. The flexibility to modify recombinant antibodies allows great applicability to various platforms for use. This review presents phage display technology, application and modifications of recombinant antibodies for diagnostics.
authors: Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia.
authors: Nur Hidayah
authors: NH
authors: Hairul Bahara
authors: Gee Jun
authors: GJ
authors: Tye
authors: Yee Siew
authors: YS
authors: Choong
authors: Eugene Boon Beng
authors: EB
authors: Ong
authors: Asma
authors: A
authors: Ismail
authors: Theam Soon
authors: TS
authors: Lim
mesh_terms: D000818:Animals; D015723:Gene Library; D006801:Humans; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D057127:Single-Chain Antibodies / Q000096:biosynthesis / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; D057127:Single-Chain Antibodies
keywords: Antibodies; Diagnostics; Phage display; scFv