title: Inhibitory inputs tune the light response properties of dopaminergic amacrine cells in mouse retina.
abstract: Dopamine (DA) is a neuromodulator that in the retina adjusts the circuitry for visual processing in dim and bright light conditions. It is synthesized and released from retinal interneurons called dopaminergic amacrine cells (DACs), whose basic physiology is not yet been fully characterized. To investigate their cellular and input properties as well as light responses, DACs were targeted for whole cell recording in isolated retina using two-photon fluorescence microscopy in a mouse line where the dopamine receptor 2 promoter drives green fluorescent protein (GFP) expression. Differences in membrane properties gave rise to cell-to-cell variation in the pattern of resting spontaneous spike activity ranging from silent to rhythmic to periodic burst discharge. All recorded DACs were light sensitive and generated responses that varied with intensity. The threshold response to light onset was a hyperpolarizing potential change initiated by rod photoreceptors that was blocked by strychnine, indicating a glycinergic amacrine input onto DACs at light onset. With increasing light intensity, the ON response acquired an excitatory component that grew to dominate the response to the strongest stimuli. Responses to bright light (photopic) stimuli also included an inhibitory OFF response mediated by GABAergic amacrine cells driven by the cone OFF pathway. DACs expressed GABA (GABA(A)α1 and GABA(A)α3) and glycine (α2) receptor clusters on soma, axon, and dendrites consistent with the light response being shaped by dual inhibitory inputs that may serve to tune spike discharge for optimal DA release.
authors: Department of Physiology & Biophysics and Program in Neurobiology & Behavior, University of Washington, Seattle, WA, USA.
authors: G S
authors: GS
authors: Newkirk
authors: M
authors: M
authors: Hoon
authors: R O
authors: RO
authors: Wong
authors: P B
authors: PB
authors: Detwiler
mesh_terms: D025042:Amacrine Cells / Q000166:cytology / Q000502:physiology*; D000818:Animals; D059290:Dopaminergic Neurons / Q000166:cytology / Q000502:physiology*; D059330:GABAergic Neurons / Q000502:physiology*; D066298:In Vitro Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010775:Photic Stimulation; D055366:Synaptic Potentials / Q000502:physiology*
keywords: amacrine cell; dopaminergic neuron; electrophysiology; immunocytochemistry; retina
references: Nature. 1989 Aug 31;340(6236):715-8
references: 2475782
references: Neuron. 1997 May;18(5):723-36
references: 9182798
references: Invest Ophthalmol. 1967 Dec;6(6):642-6
references: 6073967
references: J Comp Neurol. 1990 Nov 15;301(3):461-89
references: 1979792
references: J Neurosci. 1984 Nov;4(11):2877-90
references: 6150071
references: J Neurochem. 1981 Feb;36(2):569-79
references: 6162007
references: Nat Neurosci. 2008 Jul;11(7):790-8
references: 18552844
references: Vision Res. 2004 Dec;44(28):3235-51
references: 15535992
references: Annu Rev Physiol. 2003;65:453-80
references: 12471170
references: Vis Neurosci. 1993 Nov-Dec;10(6):1035-41
references: 7903046
references: J Neural Transm Suppl. 1995;45:61-6
references: 8748610
references: J Neurosci. 2002 Dec 15;22(24):10558-66
references: 12486148
references: Brain Res. 1984 May 28;301(1):192-7
references: 6145503
references: Curr Opin Neurobiol. 2011 Apr;21(2):269-74
references: 21353526
references: Vis Neurosci. 1992 Sep-Oct;9(3-4):271-8
references: 1327088
references: Biophys J. 2003 Oct;85(4):2158-69
references: 14507682
references: J Neurochem. 1987 Sep;49(3):916-20
references: 3039059
references: Vis Neurosci. 2007 Jul-Aug;24(4):573-80
references: 17705893
references: Neuroreport. 2004 Aug 6;15(11):1761-5
references: 15257143
references: Science. 2009 May 22;324(5930):1080-4
references: 19389999
references: Invest Ophthalmol. 1971 Jun;10(6):438-52
references: 4325307
references: Neural Dev. 2011 Aug 24;6:31
references: 21864334
references: J Neurophysiol. 2000 Oct;84(4):1697-707
references: 11024062
references: Prog Retin Eye Res. 2001 May;20(3):351-84
references: 11286897
references: J Neurosci. 1984 Nov;4(11):2866-76
references: 6150070
references: J Physiol. 1995 Dec 1;489 ( Pt 2):529-43
references: 8847645
references: Neuron. 2001 Apr;30(1):211-25
references: 11343656
references: Science. 1990 Apr 6;248(4951):73-6
references: 2321027
references: Science. 1978 Nov 24;202(4370):901-2
references: 30997
references: J Physiol. 2007 Apr 15;580(Pt. 2):397-410
references: 17255172
references: Methods Enzymol. 1992;207:100-22
references: 1528114
references: Vision Res. 1989;29(2):147-54
references: 2800344
references: J Neurochem. 1980 May;34(5):1082-6
references: 6103018
references: J Neurosci. 2007 May 30;27(22):5994-6005
references: 17537971
references: J Comp Neurol. 2005 Jul 25;488(2):113-28
references: 15924342
references: J Physiol. 1979 May;290(2):433-40
references: 469785
references: J Physiol. 1973 Oct;234(1):163-98
references: 4766219
references: Pflugers Arch. 2009 Apr;457(6):1393-414
references: 19023590
references: J Neurosci. 1998 Sep 1;18(17):6776-89
references: 9712649
references: J Neurosci. 1999 Sep 15;19(18):7812-22
references: 10479684
references: Neuroscience. 1988 Jan;24(1):19-28
references: 3368048
references: Eur J Pharmacol. 1981 Jan 29;69(3):273-9
references: 7215430
references: Proc Natl Acad Sci U S A. 1999 Jun 8;96(12):7035-40
references: 10359834
references: J Neurosci. 2010 Oct 20;30(42):14273-83
references: 20962248
references: Eur J Neurosci. 1995 Mar 1;7(3):462-9
references: 7773443
references: J Neurochem. 1988 Jan;50(1):1-6
references: 3335837
references: J Physiol. 1990 Dec;431:291-318
references: 1712843
references: Brain Res. 1988 Jun 14;452(1-2):393-5
references: 3401747
references: Vis Neurosci. 1999 Jul-Aug;16(4):653-65
references: 10431914
references: Neuron. 2008 Aug 14;59(3):486-96
references: 18701073
references: Doc Ophthalmol. 2004 Jan;108(1):17-40
references: 15104164
references: J Physiol. 1991 May;436:643-67
references: 2061849
references: Proc R Soc Lond B Biol Sci. 1987 Jun 22;231(1262):91-121
references: 2888119
references: J Physiol. 1990 Dec;431:319-42
references: 1712844
references: Cell. 2007 Aug 24;130(4):730-741
references: 17719549
references: J Pharmacol Exp Ther. 1986 Sep;238(3):1092-100
references: 3746660
references: Neuron. 2000 Dec;28(3):857-72
references: 11163272
references: Vis Neurosci. 2004 Nov-Dec;21(6):835-49
references: 15733339
references: Methods Enzymol. 1992;207:123-31
references: 1528115
references: Vis Neurosci. 1997 Mar-Apr;14(2):351-6
references: 9147486
references: Front Mol Neurosci. 2009 Jul 09;2:6
references: 19924257
references: J Neurosci. 2007 Jan 17;27(3):692-9
references: 17234601
references: Proc Natl Acad Sci U S A. 2008 Sep 16;105(37):14181-6
references: 18779590
references: Nature. 1989 Nov 9;342(6246):175-7
references: 2812013
references: J Comp Neurol. 1991 Aug 8;310(2):267-84
references: 1720142
references: Nat Neurosci. 2003 Sep;6(9):968-73
references: 12897785
references: J Neurosci. 1996 Mar 15;16(6):2127-40
references: 8604056
references: Life Sci. 1978 Oct 23;23(16):1715-27
references: 31529
references: J Physiol. 1987 Nov;392:397-416
references: 2451725
references: FEBS Lett. 1997 Nov 10;417(2):177-83
references: 9395291
references: J Physiol. 2004 Jan 15;554(Pt 2):467-82
references: 14565990
references: Vision Res. 1998 May;38(10):1411-30
references: 9667008