title: Recovery of avirulent, thermostable Newcastle disease virus strain NDV4-C from cloned cDNA and stable expression of an inserted foreign gene.
abstract: A reverse genetics system for thermostable Newcastle disease virus (NDV) is not currently available. In this study, we developed a reverse genetics system for the avirulent and thermostable NDV4-C strain. Successful recovery of NDV4-C was achieved by using either T7 RNA polymerase or cellular RNA polymerase II to drive transcription of the full-length virus antigenome from cloned cDNA. The recovered viruses rNDV4-C (T7) and rNDV4-C (CMV) showed similar growth properties, thermostability, and virulence as the parental strain NDV4-C. The potential of rNDV4-C (T7) to serve as a viral vector was assessed by generating a recombinant virus, rNDV4-eGFP, which expressed enhanced green fluorescent protein. The rNDV4-eGFP could stably carry and express eGFP for at least fifteen passages. The reverse genetics system for NDV4-C will make it possible to analyze the genetic elements that determine thermostability and the oncolytic properties of NDV.
authors: Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, No. 427 Maduan Street, Nangang District, Harbin, 150001, China.
authors: Xintao
authors: X
authors: Zhang
authors: Huairan
authors: H
authors: Liu
authors: Peixin
authors: P
authors: Liu
authors: Ben P H
authors: BP
authors: Peeters
authors: Chenchen
authors: C
authors: Zhao
authors: Xiangang
authors: X
authors: Kong
mesh_terms: D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D004279:DNA, Viral / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D015967:Gene Expression Regulation, Viral; D009522:Newcastle disease virus / Q000235:genetics* / Q000472:pathogenicity*; D014774:Virulence
chemical_list: D018076:DNA, Complementary; D004279:DNA, Viral; D012321:DNA-Directed RNA Polymerases