title: Topographically targeted osteogenesis of mesenchymal stem cells stimulated by inclusion bodies attached to polycaprolactone surfaces.
abstract: OBJECTIVE
  Bacterial inclusion bodies (IBs) are nanostructured (submicron), pseudospherical proteinaceous particles produced in recombinant bacteria resulting from ordered protein aggregation. Being mechanically stable, several physicochemical and biological properties of IBs can be tuned by appropriate selection of the producer strain and of culture conditions. It has been previously shown that IBs favor cell adhesion and surface colonization by mammalian cell lines upon decoration on materials surfaces, but how these biomaterials could influence the behavior of mesenchymal stem cells remains to be explored.
  
  
  METHODS
  Here, the authors vary topography, stiffness and wettability using the IBs to decorate polycaprolactone surfaces on which mesenchymal stem cells are cultured.
  
  
  RESULTS
  The authors show that these topographies can be used to specifically target osteogenesis from mesenchymal stem cells, and through metabolomics, they show that the cells have increased energy demand during this bone-related differentiation.
  
  
  CONCLUSIONS
  IBs as topographies can be used not only to direct cell proliferation but also to target differentiation of mesenchymal stem cells.
authors: Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, Bellaterra, Barcelona, 08193, Spain.
authors: Joaquin
authors: J
authors: Seras-Franzoso
authors: Penelope M
authors: PM
authors: Tsimbouri
authors: Karl V
authors: KV
authors: Burgess
authors: Ugutz
authors: U
authors: Unzueta
authors: Elena
authors: E
authors: Garcia-Fruitos
authors: Esther
authors: E
authors: Vazquez
authors: Antonio
authors: A
authors: Villaverde
authors: Matthew J
authors: MJ
authors: Dalby
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D005455:Fluorescent Antibody Technique; D006801:Humans; D002479:Inclusion Bodies / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis; D011091:Polyesters / Q000737:chemistry*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D011091:Polyesters; C016240:polycaprolactone