title: Ability of TEP1 in intestinal flora to modulate natural resistance of Anopheles dirus.
abstract: Blocking transmission of malaria is a reliable way to control and eliminate infection. However, in-depth knowledge of the interaction between Plasmodium and mosquito is needed. Studies suggest that innate immunity is the main mechanism inhibiting development of malaria parasites in the mosquito. Recent studies have found that use of antibiotics that inhibit the mosquito gut flora can reduce the immune response of Anopheles gambiae, thereby contributing to the development of malaria parasites. In our study, we used the non susceptible model of Anopheles dirus-Plasmodium yoelii to explore the effect of Anopheles intestinal flora on the natural resistance of A. dirus to P. yoelii. We found that in mosquitoes infected with Plasmodium, the intestinal flora can regulate expression of thioester-containing protein (TEP1) via an RNAi gene-silencing approach. Our results suggest that in the absence of TEP1, the natural microbiota cannot suppress the development of P. yoelii in A. dirus. This suggests that AdTEP1 plays an important role in the resistance of A. dirus to P. yoelii. The intestinal flora may modulate the development of P. yoelii in A. dirus by regulating TEP1 expression.
authors: Department of the Pathogenic Biology, The Third Military Medical University, Chongqing 400038, PR China.
authors: Yanyan
authors: Y
authors: Wang
authors: Ying
authors: Y
authors: Wang
authors: Jingru
authors: J
authors: Zhang
authors: Wenyue
authors: W
authors: Xu
authors: Jian
authors: J
authors: Zhang
authors: Fu Sheng
authors: FS
authors: Huang
mesh_terms: D000818:Animals; D000852:Anopheles / Q000276:immunology* / Q000382:microbiology / Q000469:parasitology; D001419:Bacteria / Q000187:drug effects / Q000254:growth & development / Q000276:immunology; D005260:Female; D020868:Gene Silencing; D019476:Insect Proteins / Q000235:genetics / Q000276:immunology*; D007303:Insect Vectors / Q000276:immunology* / Q000382:microbiology / Q000469:parasitology; D007422:Intestines / Q000382:microbiology; D010802:Phylogeny; D012330:RNA, Double-Stranded / Q000008:administration & dosage
chemical_list: D019476:Insect Proteins; D012330:RNA, Double-Stranded; C544265:TEP1 protein, Anopheles gambiae
keywords: Anopheles dirus; Intestinal flora; Plasmodium yoelii; Thioester-containing protein