title: [Changes of basic fibroblast growth factor expression in the spinal cord of rats with spared nerve injury of the sciatic nerve].
abstract: OBJECTIVE
  To observe the temporal changes of basic fibroblast growth factor (FGF-2) expression in the spinal cord of a rat model of spared nerve injury (SNI) of the sciatic nerve.
  
  
  METHODS
  A total of 100 male SD rats were randomly divided into sham-operated group and SNI group. The paw withdrawal threshold to mechanical stimulation was recorded at 1 day before and at 1, 4, 7, 14 and 28 days after the operation. The expressions of FGF-2, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the L4-6 spinal cord segments of the rats were measured at the specified time points.
  
  
  RESULTS
  The paw withdrawal threshold began to decline at 1 day after SNI, reached the lowest level at 7 days, and maintained a low level till 28 days (P<0.05). The expression of FGF-2 began to increase significantly on postoperative day 4, reached the peak level on day 14 and maintained the high level till day 28 (P<0.05). The rats with SNI showed significantly higher expressions of TNF-α and IL-6A in the spinal cord than those in the sham-operated group at each time point of measurement (P<0.05).
  
  
  CONCLUSIONS
  SNI of the sciatic nerve can induce neuropathic tactile allodynia and causes up-regulation of FGF-2 and inflammatory cytokines in the spinal cord.
authors: Department of Anesthesiology, First Affiliated Hospital of Chongqing Medical University, China. 726605323@qq.com
authors: Jiyue
authors: J
authors: You
authors: Jin
authors: J
authors: Gao
authors: Ping
authors: P
authors: Chen
authors: Lin
authors: L
authors: Sun
authors: Xiaoqiu
authors: X
authors: Yang
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D006930:Hyperalgesia; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D017288:Pain Threshold; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000293:injuries / Q000378:metabolism*; D012585:Sciatica / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D016222:Fibroblast Growth Factor 2