title: Impaired lipopolysaccharide responsiveness of cord blood mononuclear cells and the risk of asthma: a longitudinal study.
abstract: BACKGROUND
  We previously demonstrated that the proliferative response to lipopolysaccharide (LPS) in cord blood mononuclear cells (CBMCs) is negatively correlated with the induced expression of interleukin (IL)-4. Our aim, therefore, was to examine whether an impaired cellular response to LPS in infancy is associated with the risk for asthma.
  
  
  METHODS
  In a prospective cohort study, the relationship between the CBMC response to LPS and the risk of atopy and wheezing after the age of 4 y was evaluated.
  
  
  RESULTS
  LPS-induced CBMC proliferative responses varied markedly among the 102 infants studied (range, one- to fivefold increase over cells with diluent alone). Ninety-five infants (93%) were followed longitudinally. A higher CBMC proliferative response to LPS was noted in offspring born to nonatopic parents compared with those with at least one atopic parent (P = 0.008). Using a proliferative index cutoff of 2 separated patients into high and low induced IL-4 mRNA responders (P = 0.001). Significantly more children who never wheezed had a greater than twofold LPS-induced CBMC proliferative response compared to those with persistent atopic wheezing (P = 0.046).
  
  
  CONCLUSIONS
  These results demonstrate that CBMC proliferative responses to LPS is impaired in infants born to atopic parents and may be a risk factor for asthma later in life.
authors: Institute of Allergy and Immunology, Assaf Harofeh Medical Center, Zerifin, Israel. goldbergsm@yahoo.com
authors: Michael R
authors: MR
authors: Goldberg
authors: Arnon
authors: A
authors: Elizur
authors: Noemi
authors: N
authors: Luknar-Gabor
authors: Penina
authors: P
authors: Koch
authors: Joseph
authors: J
authors: Tovbin
authors: Yitzhak
authors: Y
authors: Katz
mesh_terms: D001249:Asthma / Q000453:epidemiology* / Q000209:etiology; D049109:Cell Proliferation / Q000187:drug effects; D002675:Child, Preschool; D015331:Cohort Studies; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000150:complications / Q000453:epidemiology* / Q000276:immunology*; D007073:Immunoglobulin E / Q000097:blood; D007223:Infant; D007231:Infant, Newborn; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008070:Lipopolysaccharides / Q000633:toxicity; D008137:Longitudinal Studies; D011446:Prospective Studies; D012135:Respiratory Sounds / Q000187:drug effects; D012307:Risk Factors; D018709:Statistics, Nonparametric
chemical_list: D008070:Lipopolysaccharides; D007073:Immunoglobulin E