title: A novel beta-defensin antimicrobial peptide in Atlantic cod with stimulatory effect on phagocytic activity.
abstract: A novel defensin antimicrobial peptide gene was identified in Atlantic cod, Gadus morhua. This three exon/two intron defensin gene codes for a peptide precursor consisting of two domains: a signal peptide of 26 amino acids and a mature peptide of 40 residues. The mature cod defensin has six conserved cysteine residues that form 1-5, 2-4 and 3-6 disulphide bridges. This pattern is typical of beta-defensins and this gene was therefore named cod beta-defensin (defb). The tertiary structure of Defb exhibits an α/β fold with one α helix and β1β2β3 sheets. RT-PCR analysis indicated that defb transcripts were present mainly in the swim bladder and peritoneum wall but could also be detected at moderate to low levels in skin, head- and excretory kidneys. In situ hybridisation revealed that defb was specifically expressed by cells located in the swim bladder submucosa and the oocytes. During embryonic development, defb gene transcripts were detectable from the golden eye stage onwards and their expression was restricted to the swim bladder and retina. Defb was differentially expressed in several tissues following antigenic challenge with Vibrio anguillarum, being up-regulated up to 25-fold in head kidney. Recombinant Defb displayed antibacterial activity, with a minimal inhibitory concentration of 0.4-0.8 µM and 25-50 µM against the Gram-(+) bacteria Planococcus citreus and Micrococcus luteus, respectively. In addition, Defb stimulated phagocytic activity of cod head kidney leucocytes in vitro. These findings imply that beta-defensins may play an important role in the innate immune response of Atlantic cod.
authors: Faculty of Biosciences and Aquaculture, University of Nordland, Bodø, Norway.
authors: Jareeporn
authors: J
authors: Ruangsri
authors: Yoichiro
authors: Y
authors: Kitani
authors: Viswanath
authors: V
authors: Kiron
authors: Jep
authors: J
authors: Lokesh
authors: Monica F
authors: MF
authors: Brinchmann
authors: Bård Ove
authors: BO
authors: Karlsen
authors: Jorge M O
authors: JM
authors: Fernandes
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001419:Bacteria / Q000187:drug effects / Q000276:immunology; D048389:Gadus morhua / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology; D007113:Immunity, Innate / Q000235:genetics; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010587:Phagocytosis / Q000187:drug effects* / Q000276:immunology; D010802:Phylogeny; D011487:Protein Conformation; D017421:Sequence Analysis; D023083:beta-Defensins / Q000737:chemistry / Q000235:genetics* / Q000494:pharmacology*
chemical_list: D000942:Antigens, Bacterial; D023083:beta-Defensins
references: Plant Signal Behav. 2009 Nov;4(11):1010-2
references: 20009545
references: Ann Med. 2002;34(2):96-101
references: 12108580
references: Fish Shellfish Immunol. 2010 Feb;28(2):267-74
references: 19900559
references: Fish Shellfish Immunol. 2010 May-Jun;28(5-6):879-86
references: 20153440
references: BMC Genomics. 2007 Jul 02;8:209
references: 17605782
references: BMC Genomics. 2010 Apr 30;11:278
references: 20433739
references: Dev Comp Immunol. 2003 Apr;27(4):313-21
references: 12590965
references: Biochem Biophys Res Commun. 2002 Aug 9;296(1):167-71
references: 12147245
references: Biopolymers. 2008;90(1):1-7
references: 18041067
references: Ophthalmic Res. 2009;41(2):60-75
references: 19122467
references: Fish Shellfish Immunol. 2012 Sep;33(3):522-31
references: 22705342
references: Fish Shellfish Immunol. 2011 Jan;30(1):330-9
references: 21078394
references: Mol Immunol. 2011 Jul;48(12-13):1432-8
references: 21497909
references: Mol Immunol. 2007 Jan;44(4):638-47
references: 16540171
references: Fish Shellfish Immunol. 2012 Sep;33(3):626-31
references: 22771966
references: Peptides. 2011 Feb;32(2):415-20
references: 21093512
references: Genome Biol. 2002 Jun 18;3(7):RESEARCH0034
references: 12184808
references: Curr Opin Immunol. 2002 Feb;14(1):96-102
references: 11790538
references: PLoS One. 2010 Mar 02;5(3):e9501
references: 20209162
references: Exp Oncol. 2008 Jun;30(2):153-6
references: 18566581
references: Mol Immunol. 2009 Oct;46(16):3358-66
references: 19709750
references: Comp Biochem Physiol Part D Genomics Proteomics. 2006 Mar;1(1):35-45
references: 20483233
references: Autophagy. 2007 Jul-Aug;3(4):300-22
references: 17377489
references: Dev Comp Immunol. 2006;30(12):1145-55
references: 16616955
references: Eur J Immunol. 2009 May;39(5):1353-60
references: 19404978
references: Antimicrob Agents Chemother. 2005 Dec;49(12):4957-64
references: 16304158
references: Poult Sci. 2008 Dec;87(12):2653-9
references: 19038823
references: Nature. 2011 Aug 10;477(7363):207-10
references: 21832995
references: Eur J Biochem. 2003 May;270(9):1969-79
references: 12709056
references: Comp Biochem Physiol B Biochem Mol Biol. 2008 May;150(1):23-32
references: 18302990
references: Comp Biochem Physiol Part D Genomics Proteomics. 2011 Mar;6(1):44-54
references: 20584633
references: PLoS One. 2010 Dec 20;5(12):e12883
references: 21188147
references: Fish Shellfish Immunol. 2004 Jan;16(1):1-9
references: 14675829
references: Bioinformatics. 2003 Aug 12;19(12):1572-4
references: 12912839
references: Biochem J. 2003 Jul 15;373(Pt 2):621-8
references: 12713443
references: Dev Comp Immunol. 2009 Apr;33(4):624-37
references: 19084554
references: Fish Shellfish Immunol. 2012 Mar;32(3):396-406
references: 22178249
references: Acta Histochem. 2009;111(3):176-84
references: 19193401
references: Mol Immunol. 2008 Feb;45(3):757-65
references: 17692376
references: Science. 2002 Jan 18;295(5554):457-62
references: 11799232
references: Nat Rev Immunol. 2003 Sep;3(9):710-20
references: 12949495
references: Dev Comp Immunol. 2011 Mar;35(3):385-91
references: 21093479