title: Adjuvant effect of Escherichia coli heat labile enterotoxin B subunit against internal egg contamination in domestic fowl immunised with a live Salmonella enterica serovar Enteritidis vaccine.
abstract: This study evaluated the effect of Salmonella enterica serovar Enteritidis (SE) secreting Escherichia coli heat labile enterotoxin B subunit (LTB) protein as an adjuvant for a live SE vaccine (JOL919) against virulent SE challenge in hens. The eltB gene encoding LTB was inserted into the Asd+ β-lactamase signal plasmid pJHL65. This plasmid was transformed into ΔlonΔcpxRΔasd SE to generate the LTB strain JOL1228. One-hundred female domestic fowl were divided into five groups and hens in immunised groups were primed and subsequently boosted with either JOL919 or a JOL919-JOL1228 mixture. Humoral and cellular immune responses were significantly higher in the immunised groups than the control group. On challenge with virulent SE, egg protection was 89.3% in immunised hens in group B (primed and boosted twice with JOL919 only), 89.3% in group C (primed with JOL919-JOL1228 mixture and boosted twice with JOL919), 100% in group D (primed and first booster with JOL919-JOL1228 mixture, then subsequently boosted with JOL919), 90.5% in group E (primed and boosted twice with JOL919-JOL1228 mixture) and 60.7% in group A (control group of non-immunised hens inoculated with phosphate buffered saline). The challenge strain was detected significantly less in all organs examined from hens in group D than those of the control group. These results indicate that vaccination with JOL1228, especially when added to priming and first booster immunisations, may reduce egg contamination with SE.
authors: College of Veterinary Medicine, Chonbuk National University, Jeonju 561-756, South Korea.
authors: Rahul M
authors: RM
authors: Nandre
authors: Chetan V
authors: CV
authors: Jawale
authors: John Hwa
authors: JH
authors: Lee
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D001427:Bacterial Toxins / Q000276:immunology*; D002645:Chickens; D004531:Eggs / Q000382:microbiology; D004768:Enterotoxins / Q000276:immunology*; D029968:Escherichia coli Proteins / Q000276:immunology*; D005260:Female; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D007070:Immunoglobulin A; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000276:immunology; D011201:Poultry Diseases / Q000382:microbiology / Q000517:prevention & control*; D012481:Salmonella Infections, Animal / Q000517:prevention & control*; D022562:Salmonella Vaccines / Q000276:immunology*; D012477:Salmonella enteritidis / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D007070:Immunoglobulin A; D022562:Salmonella Vaccines; C027538:heat-labile enterotoxin, E coli
keywords: Domestic fowl; Egg protection; Escherichia coli heat labile enterotoxin B; Salmonella enterica serovar Enteritidis; Vaccination