title: The distribution of interstitial cells of Cajal in congenital ureteropelvic junction obstruction.
abstract: OBJECTIVE
  The authors analysed the distribution of c-kit-positive interstitial cells of Cajal (ICCs) in obstructed ureteropelvic junction (UPJ) and its age-related changes.
  
  
  METHODS
  Twenty specimens were obtained from children with intrinsic ureteropelvic junction obstruction (UPJO), at the average age of 8.1 years (8 months-16.8 years), fixed in formalin and embedded in paraffin. Five control samples were taken from children at the average age of 2.3 years (2.4 months-7.4 years). All specimens were analysed by the immunohistochemistry test with light microscopy with respect to c-kit expression. The distribution of c-kit-positive ICCs in the two groups was compared and the correlation between the distribution of c-kit-positive ICCs and the patients' age in UPJO cases was analysed. The results were examined by Yates' χ(2) test, Mann-Whitney U test, and t test for Pearson's correlation coefficient. A P value < 0.05 was considered as statistically significant.
  
  
  RESULTS
  No statistically significant differences were found in the distribution of c-kit-positive ICCs between UPJO and the control group. No correlation was established between the age of patients with UPJO and the distribution of c-kit-positive ICCs.
  
  
  CONCLUSIONS
  No distributional difference found in obstructed and unobstructed UPJ seems to indicate that UPJO is not associated with anomalous distribution of c-kit-positive ICCs. Age-related changes in the expression of c-kit-positive ICCs are equally distributed in obstructed UPJ.
authors: Department and Clinic of Paediatric Surgery and Urology, Wroclaw Medical University, ul. M.Sklodowskiej-Curie 50/52, 50-369, Wrocław, Poland.
authors: Wojciech
authors: W
authors: Apoznanski
authors: Piotr
authors: P
authors: Koleda
authors: Zdzislaw
authors: Z
authors: Wozniak
authors: Leslaw
authors: L
authors: Rusiecki
authors: Tomasz
authors: T
authors: Szydelko
authors: Dariusz
authors: D
authors: Kalka
authors: Witold
authors: W
authors: Pilecki
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D003251:Constriction, Pathologic / Q000151:congenital / Q000473:pathology; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007150:Immunohistochemistry; D007223:Infant; D056885:Interstitial Cells of Cajal / Q000473:pathology*; D007682:Kidney Pelvis / Q000473:pathology*; D008297:Male; D014513:Ureter / Q000473:pathology*; D014517:Ureteral Obstruction / Q000151:congenital* / Q000473:pathology
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