title: Serum miR-210 and miR-30a expressions tend to revert to fetal levels in Chinese adult patients with chronic heart failure.
abstract: BACKGROUND
  MicroRNAs (miRNAs) are widely involved in the process of chronic heart failure (HF), which is characterized by reactivation of the fetal gene program. Here, we examined whether the serum expression levels of some HF-related miRNAs in adult HF patients would tend to revert to fetal levels.
  
  
  RESULTS
  Serum was obtained from the peripheral venous blood of 22 HF patients, 18 asymptomatic controls, and the umbilical venous blood of 9 fetuses from 9 independent parturitions. Serum pools of the three groups were initially screened against 40 known HF-associated miRNAs via quantitative reverse transcriptase polymerase chain reaction. Twenty-seven miRNAs were stably expressed in the serum pools. Nine miRNAs showed similar expression levels in the HF and fetus groups compared to the controls, two of which (miR-210, miR-30a) were significantly up-regulated in both groups. These miRNAs showed high diagnostic accuracy and correlations with blood N-terminal prohormone of brain natriuretic peptide, identifying them as potential biomarkers for HF. Putative targets of the miRNAs were predicted with online software programs, and the Kyoto Encyclopedia of Genes and Genomes pathway analysis was employed to identify miRNA-regulated functional modules. In particular, miR-210 seemed to be more closely related than miR-30a to the pathological mechanisms of HF, including the calcium signaling, vascular smooth muscle contraction, transforming growth factor-β signaling, and aldosterone-regulated sodium reabsorption pathways.
  
  
  CONCLUSIONS
  The serum expression levels of some HF-related miRNAs in HF patients tended towards fetal levels. Among them, miR-210 and miR-30a were elevated in the HF and fetus groups.
authors: Department of Cardiology, The second Affiliated Hospital of Nantong University, Nantong 226001, China.
authors: Dong-Sheng
authors: DS
authors: Zhao
authors: Yan
authors: Y
authors: Chen
authors: Hui
authors: H
authors: Jiang
authors: Jing-Ping
authors: JP
authors: Lu
authors: Gang
authors: G
authors: Zhang
authors: Jie
authors: J
authors: Geng
authors: Qing
authors: Q
authors: Zhang
authors: Jian-Hua
authors: JH
authors: Shen
authors: Xin
authors: X
authors: Zhou
authors: Wei
authors: W
authors: Zhu
authors: Qi-Jun
authors: QJ
authors: Shan
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002681:China; D002908:Chronic Disease; D019295:Computational Biology; D005260:Female; D005312:Fetal Blood / Q000378:metabolism*; D053263:Gene Regulatory Networks; D005819:Genetic Markers; D006333:Heart Failure / Q000097:blood / Q000175:diagnosis / Q000235:genetics*; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D020097:Natriuretic Peptide, Brain / Q000097:blood; D010446:Peptide Fragments / Q000097:blood; D011237:Predictive Value of Tests; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: D005819:Genetic Markers; C543800:MIRN210 microRNA, human; C546086:MIRN30b microRNA, human; D035683:MicroRNAs; D010446:Peptide Fragments; C109794:pro-brain natriuretic peptide (1-76); D020097:Natriuretic Peptide, Brain
keywords: Chronic heart failure; Fetus; Serum microRNAs