title: Comparison of three hydroxyapatite/β-tricalcium phosphate/collagen ceramic scaffolds: an in vivo study.
abstract: Calcium-phosphate ceramics, which have a composition similar to bone mineral, represent a potentially interesting synthetic bone graft substitute. In the present study, three porous hydroxyapatite (HA)/β-tricalcium phosphate (β-TCP)/collagen ceramic scaffolds were developed, characterized, and tested for their bone repairing capacity and osteoinductive potential in a New Zealand Rabbit model. The ratio of the ceramic components HA/-TCP/collagen varied from 40/30/30 to 50/20/30 and 60/20/20 (in wt %), respectively. None of the ceramic scaffolds succeeded in completely bridging the 6 mm calvarian defect with new bone after 60 days implantation. 60/20/20 ceramic scaffolds showed significantly more bone formation in the pores and in the periphery of the graft than the other two materials. Histomorphometric analysis revealed that the 40/30/30 scaffold produced best bone-to-implant contact (67.23 ± 0.34% with higher quality, closer contact) in comparison with 50/20/30 (54.87 ± 0.32%), and 60/20/20 (48.53 ± 0.31%). Both physicochemical and structural properties of the ceramic composites affected their in vivo behavior, either dependently or independently, emphasizing the importance of assessing bone repair parameters individually. The scaffolds may offer clinical applications in reconstructive surgery for treating bone pathologies.
authors: Implantology and Biomaterials, the University of Murcia, Murcia, Spain.
authors: José Eduardo
authors: JE
authors: Maté-Sánchez de Val
authors: Patricia
authors: P
authors: Mazón
authors: José Luis Calvo
authors: JLC
authors: Guirado
authors: Rafael Arcesio Delgado
authors: RA
authors: Ruiz
authors: María Piedad
authors: MP
authors: Ramírez Fernández
authors: Bruno
authors: B
authors: Negri
authors: Marcus
authors: M
authors: Abboud
authors: Piedad N
authors: PN
authors: De Aza
mesh_terms: D000818:Animals; D002516:Ceramics / Q000494:pharmacology*; D003094:Collagen / Q000494:pharmacology*; D006882:Hydroxyapatites / Q000494:pharmacology*; D020007:Implants, Experimental; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D019919:Prosthesis Implantation; D011817:Rabbits; D011859:Radiography; D012886:Skull / Q000000981:diagnostic imaging / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D013052:Spectrometry, X-Ray Emission; D034421:Sus scrofa; D054457:Tissue Scaffolds / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D006882:Hydroxyapatites; C074950:hydroxyapatite-beta tricalcium phosphate; D003094:Collagen
keywords: SEM; bioceramics scaffolds; collagen; histomorphometry; hydroxyapatite; tricalcium phosphate