title: Promoter polymorphisms of pri-miR-34b/c are associated with hepatocellular carcinoma.
abstract: BACKGROUND
  Numerous studies have focused on the association between miR-34 family members, which are direct p53 targets, and carcinogenesis of many cancers, including hepatocellular carcinoma (HCC). This study aimed to assess whether polymorphisms in the single-nucleotide polymorphism miR-34b/c T>C (rs4938723) and TP53 Arg72Pro (rs1042522) increase the risk of HCC and influence outcome in patients with HCC.
  
  
  METHODS
  We enrolled 157 HCC patients and 201 cancer-free control subjects from the Korean population. MicroRNA polymorphisms were genotyped using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.
  
  
  RESULTS
  We found that the miR-34b/c TC+CC frequency was significantly higher in HCC patients than in controls (adjusted odds ratio [AOR]: 1.580; 95% confidence interval [CI]: 1.029-2.426). The miR-34b/c CC-TP53 Arg/Arg combination significantly increased the risk of HCC (AOR: 13.644; 95% CI: 1.451-128.301). The SNPs miR-34b/c T>C and TP53 Arg72Pro(G>C) had no influence on survival of HCC patients.
  
  
  CONCLUSIONS
  Our findings suggest that loss of the T allele in miR-34b/c T>C, and the miR-34b/c CC-TP53 Arg/Arg combination increases the risk of HCC in the Korean population.
authors: Department of Internal Medicine, CHA Bundang Medical Center, CHA University, Seongnam, South Korea.
authors: Myung Su
authors: MS
authors: Son
authors: Moon Ju
authors: MJ
authors: Jang
authors: Young Joo
authors: YJ
authors: Jeon
authors: Won Hee
authors: WH
authors: Kim
authors: Chang-Il
authors: CI
authors: Kwon
authors: Kwang Hyun
authors: KH
authors: Ko
authors: Pil Won
authors: PW
authors: Park
authors: Sung Pyo
authors: SP
authors: Hong
authors: Kyu Sung
authors: KS
authors: Rim
authors: Sung Won
authors: SW
authors: Kwon
authors: Seong Gyu
authors: SG
authors: Hwang
authors: Nam Keun
authors: NK
authors: Kim
mesh_terms: D000368:Aged; D000483:Alleles; D044466:Asian People / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D060005:Genotyping Techniques; D006801:Humans; D053208:Kaplan-Meier Estimate; D008113:Liver Neoplasms / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009367:Neoplasm Staging; D016017:Odds Ratio; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012189:Retrospective Studies; D012307:Risk Factors; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
chemical_list: C522425:MIRN34 microRNA, human; D035683:MicroRNAs; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53