title: Detection of miRNA in live cells by using templated RuII-catalyzed unmasking of a fluorophore.
abstract: Reactions templated by cellular nucleic acids are attractive for nucleic acid sensing or responsive systems. Herein we report the use of a photocatalyzed reductive cleavage of an immolative linker to unmask a rhodamine fluorophore, and its application to miRNA imaging. The reaction was found to proceed with a very high turnover (>4000) and provided reliable detection down to 5 pM of template by using γ-serine-modified peptide nucleic acid (PNA) probes. The reaction was used for the selective detection of miR-21 in BT474 cells and miR-31 in HeLa cells following irradiation for 30 min. The probes were introduced by using reversible permeation with streptolysin-O (SLO) or a transfection technique.
authors: Institut de Science et Ingénierie Supramoléculaires (ISIS-UMR 7006), Université de Strasbourg, CNRS, 8 allée Gaspard Monge, 67000 Strasbourg, France.
authors: Kalyan K
authors: KK
authors: Sadhu
authors: Nicolas
authors: N
authors: Winssinger
mesh_terms: D002384:Catalysis; D045744:Cell Line, Tumor; D002470:Cell Survival; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D035683:MicroRNAs / Q000032:analysis* / Q000737:chemistry; D012235:Rhodamines / Q000737:chemistry; D012428:Ruthenium / Q000737:chemistry*; D014162:Transfection
chemical_list: D005456:Fluorescent Dyes; D035683:MicroRNAs; D012235:Rhodamines; D012428:Ruthenium