title: Identification and gene expression analyses of natriuretic peptide system in the ovary of goat (Capra hircus).
abstract: Natriuretic peptides (NPs) are involved in maintaining cardiovascular and fluid homeostasis, regulating reproductive processes and bone growth, and other numerous functions. To better understand the role of NPs in goat (Capra hircus), in the present study, full-length cDNAs of goat Nppa (natriuretic peptide precursor A), Nppb (natriuretic peptide precursor B) and Nppc (natriuretic peptide precursor C), respectively encoding ANP, BNP and CNP, were cloned from adult goat heart and ovary. The putative prepropeptide ANP (prepro-ANP) and prepro-CNP share a high amino acid sequence identity with other species. Real-time PCR showed that Nppa, Nppb and Nppc were widely expressed in adult goat tissues. The mRNA expression of Nppa and Nppb in the heart was extremely higher compared with other tissues. Nppc mRNA expression in the lung and uterus was also higher than in other tissues. The expression of Nppa, Nppb and Nppc genes was examined at different ovarian follicle stages using RT-PCR. The mRNAs of Nppa and Nppb were detected in secondary follicles as well as in COCs (cumulus-oocyte-complexes) and granulosa cells of antral follicles. However, the mRNA expression of Nppc was observed throughout ovarian follicle development, and it was especially higher in granulosa cells of antral follicles. In vitro, stimulating goat granulosa cells with FSH led to an increase in the expression of Nppc by dose- and time-dependent manners and a rapid decline was induced by LH stimulation, but the expression of Nppa and Nppb did not change after FSH or LH treatment. These results suggest that Nppc is a gonadotropin-induced gene in granulosa cells of goat ovary and CNP may be involved in the regulation of ovarian follicle development and oocyte maturation.
authors: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors: Jia-Yin
authors: JY
authors: Peng
authors: Hai-Yun
authors: HY
authors: Xin
authors: Peng
authors: P
authors: Han
authors: Hai-Bo
authors: HB
authors: Zhao
authors: Long
authors: L
authors: Bai
authors: Xiao-Peng
authors: XP
authors: An
authors: Bin-Yun
authors: BY
authors: Cao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology; D005786:Gene Expression Regulation; D006041:Goats / Q000235:genetics* / Q000378:metabolism; D006107:Granulosa Cells / Q000187:drug effects; D008168:Lung / Q000378:metabolism; D008969:Molecular Sequence Data; D020097:Natriuretic Peptide, Brain / Q000235:genetics; D020098:Natriuretic Peptide, C-Type / Q000235:genetics*; D009865:Oocytes / Q000166:cytology / Q000378:metabolism; D010053:Ovary / Q000166:cytology* / Q000378:metabolism; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013997:Time Factors
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D020097:Natriuretic Peptide, Brain; D020098:Natriuretic Peptide, C-Type; D009320:Atrial Natriuretic Factor; D005640:Follicle Stimulating Hormone