title: Modification of antioxidative and antiapoptotic genes expression in irradiated K562 cells upon fullerenol C60(OH)24 nanoparticle treatment.
abstract: Recent data established the prospective applications for fullerenol (C60(OH)24) nanoparticle (FNP) in many fields, such as antioxidants, neuroprotective agents, and potential anti-radiation drugs. Leukemia cell sensitization to apoptosis induced by ionizing radiation is achieved by upregulation of ROS production and/or downregulation of antioxidative enzymes. Therefore, our aim was to analyze the potential role of fullerenol nanoparticle in modulation of the leukemic cellular response to irradiation. We used the qRT-PCR to analyze the expression level of mRNA for 11 genes in irradiated and FNP pre-treated irradiated K562 cells, and compared the gene expression level with the overall cell survival. Our results of the improved cell survival in FNP-treated irradiated cells and significant overexpression of anti-apoptotic Bcl-2 and Bcl-xL and cytoprotective genes such as GSTA4, MnSOD, NOS, CAT and HO-1 genes, may indicate that FNP exerts cytoprotective function in K562 leukemic cells, rendering K562 cells more tolerant to radiotherapy.
authors: Medical Faculty Novi Sad, Clinical Center of Vojvodina, University of Novi Sad, Hajduk Veljkova 1, 21000 Novi Sad, Serbia.
authors: Karmen
authors: K
authors: Stankov
authors: Ivana
authors: I
authors: Borisev
authors: Vesna
authors: V
authors: Kojic
authors: Lazar
authors: L
authors: Rutonjski
authors: Gordana
authors: G
authors: Bogdanovic
authors: Aleksandar
authors: A
authors: Djordjevic
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D002470:Cell Survival / Q000187:drug effects* / Q000528:radiation effects*; D037741:Fullerenes / Q000008:administration & dosage*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D020014:K562 Cells; D053758:Nanoparticles / Q000008:administration & dosage*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D011837:Radiation-Protective Agents / Q000008:administration & dosage; D051020:bcl-X Protein / Q000378:metabolism*
chemical_list: D000975:Antioxidants; C494811:BCL2L1 protein, human; D037741:Fullerenes; D019253:Proto-Oncogene Proteins c-bcl-2; D011837:Radiation-Protective Agents; D051020:bcl-X Protein; C069837:fullerene C60