title: R gene expression changes related to Cercospora hydrangeae L.
abstract: Nursery growing as well as common landscape hydrangeas are all susceptible to leaf spot fungus Cercospora hydrangeae. Warm and rainy weather causes the fungal spores to germinate quickly and spread over the plant leaves forming small purple or brown spots. Although Hydrangea plants are not killed by leaf spot, it detracts from the value of plants through the reduction of flowering and plant vigor. The aim of our study was to isolate, characterize and investigate the expression profile of Hydrangea macrophylla resistance (R) gene transcripts under C. hydrangeae fungus infection and examine their evolutionary relationships by phylogenetic analysis. R-genes are thought to be one of the components of the genetic resistance in plants and most of them encode nucleotide binding site-leucine rich repeat (NBS-LRR) proteins. A cDNA-NBS strategy was carried out using as template cDNAs isolated from control and infected plant leaves. The cDNA-NBS profiling gave an excellent bands reproducibility. Twenty new transcripts corresponding to NBS-LRR proteins were identified only in infected plants. The extent of positivity between the aminoacid sequences at NBS region varied from 45 to 90 %, which indicates the diversity among the RGAs. The results of this paper will provide a genomic framework for the further isolation of candidate disease resistance NBS-encoding genes in Hortensia, and contribute to the understanding of the evolutionary mode of NBS-encoding genes in Hydrangeaceae crops.
authors: Dipartimento di Scienze e Tecnologie Ambientali Biologiche e Farmaceutiche, Seconda Università di Napoli, via Vivaldi 43, 81100, Caserta, Italy.
authors: Ioannis
authors: I
authors: Kafantaris
authors: Pasqualina
authors: P
authors: Woodrow
authors: Petronia
authors: P
authors: Carillo
mesh_terms: D001203:Ascomycota / Q000502:physiology*; D001665:Binding Sites; D018076:DNA, Complementary; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D031966:Hydrangea / Q000145:classification / Q000235:genetics* / Q000382:microbiology*; D000090263:Leucine-Rich Repeat Proteins; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology*; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D018076:DNA, Complementary; D000090263:Leucine-Rich Repeat Proteins; D011506:Proteins
references: Plant Cell. 2006 Aug;18(8):2082-93
references: 16844906
references: Genome Biol. 2006;7(4):212
references: 16677430
references: BMC Plant Biol. 2007 Oct 23;7:56
references: 17956627
references: Plant Cell. 2003 Apr;15(4):809-34
references: 12671079
references: Annu Rev Plant Biol. 2003;54:23-61
references: 14502984
references: Mol Plant Microbe Interact. 1998 Oct;11(10):968-78
references: 9768514
references: Plant Cell. 2002 Nov;14(11):2929-39
references: 12417711
references: Plant J. 2005 Oct;44(2):208-22
references: 16212601
references: Proc Natl Acad Sci U S A. 2007 Sep 11;104(37):14861-6
references: 17804803
references: Mol Biol Rep. 2011 Aug;38(6):3787-94
references: 21104438
references: Plant J. 2002 Feb;29(4):439-51
references: 11846877
references: J Exp Bot. 2005 Feb;56(412):567-75
references: 15545294
references: Nature. 2006 Nov 16;444(7117):323-9
references: 17108957
references: Mol Genet Genomics. 2004 May;271(4):402-15
references: 15014983
references: Nature. 2001 Jun 14;411(6839):826-33
references: 11459065
references: Plant Cell. 1996 Oct;8(10):1773-91
references: 8914325
references: Genet Mol Res. 2011 Oct 25;10(4):2637-52
references: 22057959
references: Am J Bot. 2008 Mar;95(3):393-8
references: 21632363
references: Nat Rev Genet. 2007 May;8(5):382-93
references: 17404584
references: Cell. 2006 Feb 24;124(4):803-14
references: 16497589
references: Plant Cell. 2008 Aug;20(8):2009-17
references: 18723576