title: [The effects of low-frequency electric stimulus on hippocampal of Effects of low-frequency electric stimulus on hippocampal of α5 subunit of extra synapse GABAA receptor in kainic acid-induced epilepsy rats].
abstract: OBJECTIVE
  To explore the effects of low-frequency electric stimulus (LFS) on hippocampal of α5 subunit of extrasynaptic GABAA receptor in kainic acid-induced epilepsy rats and explore the possible mechanism of LFS on hippocampus to treat epilepsy.
  
  
  METHODS
  A total of 32 male Sprague-Dawley rats were divided randomly into 4 groups:epileptic, pseudo-surgery, LFS treatment and control. The expression of α5 subunit of extrasynaptic GABAA receptor and the association with the curative effects of LFS were evaluated by behavioristics, real-time fluorogenic quantitative polymerase chain reaction (PCR) and Western blot.
  
  
  RESULTS
  After treatment, the number of seizures in LFS group were significantly lower than that in the epileptic and pseudo-surgery groups (2.88 ± 0.83 vs 8.50 ± 0.93 and 8.88 ± 0.83) (P < 0.01). The results of real-time PCR demonstrated that the expression of mRNA of α5 subunits in LFS treatment group was obviously higher than that in epileptic and pseudo-surgery groups (0.74 ± 0.20 vs 0.30 ± 0.16 and 0.31 ± 0.16) (P < 0.01), but was lower than 1.10 ± 0.23 in control group. Western blot demonstrated that the protein expression of α5 subunits in LFS (0.75 ± 0.09) and control (0.88 ± 0.09) groups were dramatically higher than that in epileptic (0.22 ± 0.08) and pseudo-surgery (0.26 ± 0.08) groups (P < 0.01).
  
  
  CONCLUSIONS
  Low-frequency electric stimulus on hippocampus is effective in controlling epileptic seizures. And the mechanism may be related with the expression level of α5 subunits of extrasynaptic GABAA receptor.
authors: Ningxia Medical University, Incubation Base of National Key Laboratory for Cerebrocranial Diseases, Yinchuan 750004, China.
authors: Fa-zheng
authors: FZ
authors: Shen
authors: Feng
authors: F
authors: Wang
authors: Guang-ming
authors: GM
authors: Yang
authors: Lin
authors: L
authors: Miao
authors: En-jie
authors: EJ
authors: Wang
authors: Jin
authors: J
authors: Xu
authors: Yong-long
authors: YL
authors: Xu
authors: Tao
authors: T
authors: Sun
mesh_terms: D000818:Animals; D004558:Electric Stimulation / Q000379:methods*; D004827:Epilepsy / Q000139:chemically induced / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism*; D007608:Kainic Acid / Q000009:adverse effects; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D011963:Receptors, GABA-A / Q000378:metabolism*
chemical_list: D011963:Receptors, GABA-A; D007608:Kainic Acid