title: Surface modification of electrospun polyvinylidene fluoride nanofiber membrane by plasma treatment for protein detection.
abstract: Electrospun polyvinylidene fluoride (PVDF) nanofiber membranes have 3-dementional (3-D) open pore channel and hence have excellent application potential in Western blot. In this study we have modified electrospun PVDF nanofiber membrane by argon (Ar) plasma treatment to improve the surface hydrophilic and detection sensitivity. The results showed that the detection sensitivity of the Ar plasma-treated PVDF nanofiber membrane increased with increasing plasma treatment time without the need for a methanol pre-wet step. This suggests that the Ar plasma treated PVDF nanofiber membrane can be useful in Western blot with high sensitivity and without methanol pre-wet step.
authors: Department of Oral Biochemistry, School of Dentistry, Chosun University, Gwangju 501-759, Republic of Korea.
authors: Eugene
authors: E
authors: Cho
authors: Chan
authors: C
authors: Kim
authors: Ju-Young
authors: JY
authors: Park
authors: Cheol Ho
authors: CH
authors: Hwang
authors: Jin Hee
authors: JH
authors: Kim
authors: Yoong Ahm
authors: YA
authors: Kim
authors: Morinobu
authors: M
authors: Endo
authors: Duck Rye
authors: DR
authors: Chang
authors: Joong-Ki
authors: JK
authors: Kook
mesh_terms: D015153:Blotting, Western / Q000295:instrumentation*; D004595:Electroplating / Q000379:methods*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008567:Membranes, Artificial; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure*; D010316:Particle Size; D058626:Plasma Gases / Q000737:chemistry; D011145:Polyvinyls / Q000737:chemistry*; D040081:Protein Array Analysis / Q000295:instrumentation; D011506:Proteins / Q000032:analysis*; D012399:Rotation; D013499:Surface Properties; D005465:Fluorocarbon Polymers
chemical_list: D008567:Membranes, Artificial; D058626:Plasma Gases; D011145:Polyvinyls; D011506:Proteins; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers