title: Tespa1 is a novel inositol 1,4,5-trisphosphate receptor binding protein in T and B lymphocytes.
abstract: Tespa1 has been recently reported to be a critical molecule in T-cell development, however, the precise molecular mechanisms of Tespa1 remain elusive. Here, we demonstrate that Tespa1 shows amino-acid sequence homology to KRAS-induced actin-interacting protein (KRAP), an inositol 1,4,5-trisphosphate receptor (IP3R) binding protein, and that Tespa1 physically associates with IP3R in T and B lymphocytes. Two-consecutive phenylalanine residues (Phe185/Phe186) in Tespa1, which are conserved between Tespa1 and KRAP, are indispensable for the association between Tespa1 and IP3R. These findings suggest that Tespa1 plays critical roles in the immune system through the regulation of the IP3R.
authors: Department of Cell Biology, Faculty of Medicine, Fukuoka University, 7-45-1 Nanakuma, Jonan-ku, Fukuoka 814-0180, Japan.
authors: Hiroshi
authors: H
authors: Matsuzaki
authors: Takahiro
authors: T
authors: Fujimoto
authors: Takeharu
authors: T
authors: Ota
authors: Masahiro
authors: M
authors: Ogawa
authors: Toshiyuki
authors: T
authors: Tsunoda
authors: Keiko
authors: K
authors: Doi
authors: Masato
authors: M
authors: Hamabashiri
authors: Masatoshi
authors: M
authors: Tanaka
authors: Senji
authors: S
authors: Shirasawa
keywords: IP3R; IP3R, Inositol 1,4,5-trisphosphate receptor; KRAP, KRAS-induced actin-interacting protein.; Lymphocyte; Protein–protein interaction; Spleen; Tespa1; Tespa1, thymocyte-expressed positive selection-associated 1; Thymus
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