title: [Immunoregulation of autologous peripheral blood stem cell transplantation in patients with hepatitis B-related end-stage liver disease].
abstract: OBJECTIVE
  To investigate the immunoregulatory effect of autologous peripheral blood stem cells (PBSCs) transplantation on T lymphocytes and cytokines in patients with HBV-related end-stage liver disease.
  
  
  METHODS
  Flow cytometry was used to measure the percentages of Th1, Th2 and regulatory T cells (Treg) in peripheral blood. Enzyme-linked immunosorbent assay (ELISA) was performed to analyze the levels of serum IFN-γ, IL-6 and IL-10.
  
  
  RESULTS
  Patients with HBV-related end-stage liver disease displayed significantly improved liver function after PBSCs transplantation. Statistically, after PBSCs transplantation, the percentages of Th2 and Treg in peripheral blood markedly increased, but serum IL-6 and IL-10 declined significantly. No significant differences were observed in the changes of Th1 and its cytokine, IFN-γ after transplantation.
  
  
  CONCLUSIONS
  Autologous PBSCs transplantation can depress inflammation in liver by regulating immune microenvironment, which at least in part delineates the mechanism of stem cells-mediated therapeutic benefit on end-stage liver disease.
authors: State Key Laboratory of Cancer Biology, Xijing Hospital of Digestive Disease, Xijing Hospital of Digestive Disease, Fourth Military Medical University, Xi'an 710032, China. jlh860611@163.com
authors: Linhua
authors: L
authors: Zheng
authors: Jindong
authors: J
authors: Chu
authors: Ling
authors: L
authors: Tan
authors: Yongquan
authors: Y
authors: Shi
authors: Ying
authors: Y
authors: Han
mesh_terms: D058625:End Stage Liver Disease / Q000276:immunology* / Q000821:virology*; D005260:Female; D006509:Hepatitis B / Q000276:immunology*; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology; D016753:Interleukin-10 / Q000276:immunology; D015850:Interleukin-6 / Q000276:immunology; D008297:Male; D008875:Middle Aged; D036102:Peripheral Blood Stem Cell Transplantation / Q000379:methods*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology; D014181:Transplantation Immunology; D014182:Transplantation, Autologous
chemical_list: D015850:Interleukin-6; D016753:Interleukin-10; D007371:Interferon-gamma