title: Reactive oxygen species production and redox state in parthenogenetic and sperm-mediated bovine oocyte activation.
abstract: The knowledge concerning redox and reactive oxygen species (ROS)-mediated regulation of early embryo development is scarce and remains controversial. The aim of this work was to determine ROS production and redox state during early in vitro embryo development in sperm-mediated and parthenogenetic activation of bovine oocytes. Sperm-mediated oocyte activation was carried out in IVF-modified synthetic oviductal fluid (mSOF) with frozen-thawed semen. Parthenogenetic activation was performed in TALP plus ionomycin and then in IVF-mSOF with 6-dimethylaminopurine plus cytochalasin B. Embryos were cultured in IVF-mSOF. ROS and redox state were determined at each 2-h interval (7-24 h from activation) by 2',7'-dichlorodihydrofluorescein diacetate and RedoxSensor Red CC-1 fluorochromes respectively. ROS levels and redox state differed between activated and non-activated oocytes (P<0.05 by ANOVA). In sperm-activated oocytes, an increase was observed between 15 and 19 h (P<0.05). Conversely, in parthenogenetically activated oocytes, we observed a decrease at 9 h (P<0.05). In sperm-activated oocytes, ROS fluctuated throughout the 24 h, presenting peaks around 7, 19, and 24 h (P<0.05), while in parthenogenetic activation, peaks were detected at 7, 11, and 17 h (P<0.05). In the present work, we found clear distinctive metabolic patterns between normal and parthenogenetic zygotes. Oxidative activity and ROS production are an integral part of bovine zygote behavior, and defining a temporal pattern of change may be linked with developmental competence.
authors: Area of Biochemistry, School of Veterinary Sciences, Institute of Research and Technology in Animal Reproduction, Facultad de Ciencias Veterinarias, University of Buenos Aires, Buenos Aires, Argentina. smorado@fvet.uba.ar
authors: S
authors: S
authors: Morado
authors: P
authors: P
authors: Cetica
authors: M
authors: M
authors: Beconi
authors: J G
authors: JG
authors: Thompson
authors: G
authors: G
authors: Dalvit
mesh_terms: D000003:Abattoirs; D000818:Animals; D017849:Animals, Inbred Strains; D002417:Cattle / Q000502:physiology*; D048750:Cell Nucleus Division; D015925:Cryopreservation / Q000662:veterinary; D004477:Ectogenesis; D046149:Embryo Culture Techniques / Q000662:veterinary; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary; D059471:In Vitro Oocyte Maturation Techniques / Q000662:veterinary; D007700:Kinetics; D008297:Male; D009865:Oocytes / Q000166:cytology / Q000502:physiology*; D010084:Oxidation-Reduction; D010312:Parthenogenesis; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012662:Semen Preservation / Q000662:veterinary; D013084:Sperm-Ovum Interactions; D015053:Zygote / Q000166:cytology / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species