title: Microinjecting holo-aequorin into dechorionated and intact zebrafish embryos.
abstract: The injection of holo-aequorin into embryos at the one-cell stage, along with the use of a simple photomultiplier tube or luminescence imaging system, allows transient localized elevations of free cytosolic Ca(2+) to be recorded and observed during the first 24 h of zebrafish development. The technique for loading dechorionated or intact one-cell stage zebrafish embryos with holo-aequorin is described here.
authors: Division of Life Science and State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science & Technology, Clear Water Bay, Kowloon, Hong Kong, People's Republic of China. barnie@ust.hk
authors: Sarah E
authors: SE
authors: Webb
authors: Andrew L
authors: AL
authors: Miller
mesh_terms: D000331:Aequorin / Q000008:administration & dosage*; D000818:Animals; D002118:Calcium / Q000032:analysis; D015509:Developmental Biology / Q000379:methods*; D008845:Microinjections / Q000379:methods*; D015398:Signal Transduction; D015027:Zebrafish / Q000196:embryology*
chemical_list: D000331:Aequorin; D002118:Calcium