title: Transferability of simple sequence repeat (SSR) markers developed in guava (Psidium guajava L.) to four Myrtaceae species.
abstract: Present study demonstrated the cross-genera transferability of 23 simple sequence repeat (SSR) primer pairs developed for guava (Psidium guajava L.) to four new targets, two species of eucalypts (Eucalyptus citriodora, Eucalyptus camaldulensis), bottlebrush (Callistemon lanceolatus) and clove (Syzygium aromaticum), belonging to the family Myrtaceae and subfamily Myrtoideae. Off the 23 SSR loci assayed, 18 (78.2%) gave cross-amplification in E. citriodora, 14 (60.8%) in E. camaldulensis and 17-17 (73.9%) in C. lanceolatus and S. aromaticum. Eight primer pairs were found to be transferable to all four species. The number of alleles detected at each locus ranged from one to nine, with an average of 4.8, 2.6, 4.5 and 4.6 alleles in E. citriodora, E. camaldulensis, C. lanceolatus and S. aromaticum, respectively. The high levels of cross-genera transferability of guava SSRs may be applicable for the analysis of intra- and inter specific genetic diversity of target species, especially in E. citriodora, C. lanceolatus and S. aromaticum, for which till date no information about EST-derived as well as genomic SSR is available.
authors: Department of Botany, Biotechnology Centre, Jai Narain Vyas University, Jodhpur, 342033, Rajasthan, India. mkraibhu@gmail.com
authors: Manoj K
authors: MK
authors: Rai
authors: Mahendra
authors: M
authors: Phulwaria
authors: N S
authors: NS
authors: Shekhawat
mesh_terms: D017931:DNA Primers / Q000235:genetics; D005787:Gene Frequency; D018014:Gene Transfer Techniques; D005819:Genetic Markers / Q000235:genetics*; D014644:Genetic Variation; D018895:Microsatellite Repeats / Q000235:genetics*; D027822:Myrtaceae / Q000235:genetics*; D016133:Polymerase Chain Reaction; D031647:Psidium / Q000235:genetics*
chemical_list: D017931:DNA Primers; D005819:Genetic Markers
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