title: Effects of a single nucleotide polymorphism in the leptin gene on the productive traits of dairy buffaloes (Bubalus bubalis).
abstract: The gene responsible for coding the leptin hormone has been associated with productive and reproductive traits in cattle. In dairy cattle, different polymorphisms found in the leptin gene have been associated with several traits of economic interest, such as energy balance, milk yield and composition, live weight, fertility and dry matter consumption. The aim of this study was to detect genetic variability in the leptin gene of buffaloes and to test possible associations with milk yield, fat and protein percentages, age at first calving and first calving interval. Three genotypes (AA, AG and GG) were identified by polymerase chain reaction-restriction fragment length polymorphism, which presented genotypic frequencies of 0.30, 0.54 and 0.16, respectively. The allele frequencies were 0.57 for the A allele and 0.43 for the G allele. No significant effects were found in the present study, but there is an indicative that leptin gene affects lipid metabolism.
authors: Animal Sciences Departament, São Paulo State University, Campus Jaboticabal, Via de Acesso Prof. Paulo Donato Castellane, s/n-CEP, Jaboticabal, SP, 14884-900, Brazil. lzetouni@gmail.com
authors: Larissa
authors: L
authors: Zetouni
authors: Gregório Miguel Ferreira
authors: GM
authors: de Camargo
authors: Patrícia Dias
authors: PD
authors: da Silva Fonseca
authors: Fernanda Maria Monsalves
authors: FM
authors: Gil
authors: Naudin Alejandro Hurtado
authors: NA
authors: Lugo
authors: Rusbel Raul
authors: RR
authors: Aspilcueta-Borquis
authors: Marcelo
authors: M
authors: Cervini
authors: Humberto
authors: H
authors: Tonhati
mesh_terms: D000818:Animals; D002020:Buffaloes / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D005787:Gene Frequency; D005838:Genotype; D020738:Leptin / Q000235:genetics*; D008892:Milk / Q000706:statistics & numerical data*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: D017931:DNA Primers; D020738:Leptin
references: J Dairy Sci. 2010 Feb;93(2):784-91
references: 20105550
references: Reprod Fertil Dev. 2000;12(7-8):405-11
references: 11545180
references: Anim Genet. 2004 Dec;35(6):462-3
references: 15566470
references: J Dairy Res. 2012 Nov;79(4):429-35
references: 22994977
references: Anim Genet. 2005 Apr;36(2):111-8
references: 15771719
references: Mol Biol Rep. 2012 Sep;39(9):8733-40
references: 22722989
references: J Reprod Fertil Suppl. 1995;49:477-92
references: 7623336
references: J Anim Sci. 2010 Feb;88(2):435-41
references: 19820053
references: J Dairy Sci. 2010 May;93(5):2195-201
references: 20412935
references: BMC Genet. 2010 Jul 29;11:73
references: 20670403
references: Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7
references: 271968
references: Domest Anim Endocrinol. 2005 Jul;29(1):3-22
references: 15876510
references: Trop Anim Health Prod. 2012 Oct;44(7):1587-92
references: 22395794
references: Genet Sel Evol. 2002 Jan-Feb;34(1):105-16
references: 11929627
references: J Dairy Sci. 2007 Sep;90(9):4022-32
references: 17699018
references: Nature. 1994 Dec 1;372(6505):425-32
references: 7984236
references: J Dairy Sci. 2008 Aug;91(8):3190-200
references: 18650297
references: J Dairy Sci. 2002 Jun;85(6):1633-8
references: 12146498
references: J Dairy Sci. 2011 Jul;94(7):3618-28
references: 21700051