title: Cell proliferation is promoted by compressive stress during early stage of chondrogenic differentiation of rat BMSCs.
abstract: The presence of an appropriate number of viable cells is prerequisite for successive differentiation during chondrogenesis. Chondrogenic differentiation has been reported to be influenced by mechanical stimuli. This research aimed to study the effects of cyclic compressive stress on cell viability of rat bone marrow-derived MSCs (BMSCs) during chondrogenesis as well as its underlying mechanisms. The results showed that dynamic compression increased cell quantity and viability remarkably in the early stage of chondrogenesis, during which the expression of Ihh, Cyclin D1, CDK4, and Col2α1 were enhanced significantly. Possible signal pathways implicated in the process were explored in our study. MEK/ERK and p38 MAPK were not found to function in this process while BMP signaling seemed to play an important role in the mechanotransduction during chondrogenic proliferation. In conclusion, dynamic compressive stress could enhance cell viability during chondrogenesis, which might be achieved by activating BMP signaling.
authors: Department of Orthodontics, State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, West China School of Stomatology, Sichuan University, Chengdu, 610041, China.
authors: Yating
authors: Y
authors: Wang
authors: Jun
authors: J
authors: Wang
authors: Ding
authors: D
authors: Bai
authors: Jinlin
authors: J
authors: Song
authors: Rui
authors: R
authors: Ye
authors: Zhihe
authors: Z
authors: Zhao
authors: Lei
authors: L
authors: Lei
authors: Jin
authors: J
authors: Hao
authors: Chunmiao
authors: C
authors: Jiang
authors: Shanbao
authors: S
authors: Fang
authors: Shu
authors: S
authors: An
authors: Qian
authors: Q
authors: Cheng
authors: Juan
authors: J
authors: Li
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D020219:Chondrogenesis / Q000502:physiology*; D024043:Collagen Type II; D019938:Cyclin D1 / Q000096:biosynthesis; D051358:Cyclin-Dependent Kinase 4 / Q000096:biosynthesis; D053823:Hedgehog Proteins / Q000096:biosynthesis; D020935:MAP Kinase Signaling System; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D051381:Rats; D013314:Stress, Mechanical; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: C511513:COL2A1 protein, rat; D024043:Collagen Type II; D053823:Hedgehog Proteins; C550919:Ihh protein, rat; D019938:Cyclin D1; C495903:Cdk4 protein, rat; D051358:Cyclin-Dependent Kinase 4; D048051:p38 Mitogen-Activated Protein Kinases