title: Purification of pregnancy-associated glycoproteins from late-pregnancy Bubalus bubalis placentas and development of a radioimmunoassay for pregnancy diagnosis in water buffalo females.
abstract: BACKGROUND
  Pregnancy-associated glycoproteins (PAGs) were first described as placental antigens present in the blood serum of the mother soon after implantation. Here, we describe the purification of several pregnancy-associated glycoproteins from water buffalo placenta (wbPAGs). A specific radioimmunoassay (RIA) was developed for early pregnancy diagnosis in buffalo species.
  
  
  RESULTS
  Amino-terminal microsequencing of immunoreactive placental proteins allowed the identification of eleven wbPAGs sequences [Swiss-Prot accession numbers: P86369 to P86379]. Three polyclonal antisera (AS#858, AS#859 and AS#860) were raised in rabbits against distinct wbPAG fractions. A new RIA (RIA-860) was developed and used to distinguish between pregnant (n=33) and non-pregnant (n=26) water buffalo females.
  
  
  CONCLUSIONS
  Our results confirmed the multiplicity of PAG expression in buffalo placenta. In addition, the RIA-860 system was shown to be sensitive, linear, reproducible, accurate and specific in measuring PAG concentrations in buffalo plasma samples from Day 37 of gestation onwards.
authors: Department of Biopathological Veterinary Science, Faculty of Veterinary Medicine, University of Perugia, Perugia, 06126, Italy. barbato@unipg.it
authors: Olimpia
authors: O
authors: Barbato
authors: Noelita
authors: N
authors: Melo de Sousa
authors: Vittoria Lucia
authors: VL
authors: Barile
authors: Claudio
authors: C
authors: Canali
authors: Jean-François
authors: JF
authors: Beckers
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002020:Buffaloes / Q000378:metabolism*; D005260:Female; D006023:Glycoproteins / Q000097:blood / Q000276:immunology / Q000302:isolation & purification*; D007106:Immune Sera / Q000276:immunology; D008969:Molecular Sequence Data; D010920:Placenta / Q000737:chemistry*; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000097:blood / Q000276:immunology / Q000302:isolation & purification*; D011259:Pregnancy Tests, Immunologic / Q000379:methods / Q000662:veterinary*; D011817:Rabbits / Q000276:immunology; D011863:Radioimmunoassay / Q000379:methods / Q000662:veterinary*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D006023:Glycoproteins; D007106:Immune Sera; D011257:Pregnancy Proteins
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