title: Production of Japanese encephalitis virus-like particles in insect cells.
abstract: Virus-like particles (VLPs) are composed of one or several recombinant viral surface proteins that spontaneously assemble into particulate structures without the incorporation of virus DNA or RNA. The baculovirus-insect cell system has been used extensively for the production of recombinant virus proteins including VLPs. While the baculovirus-insect cell system directs the transient expression of recombinant proteins in a batch culture, stably transformed insect cells allow constitutive production. In our recent study, a secretory form of Japanese encephalitis (JE) VLPs was successfully produced by Trichoplusia ni BTI-TN-5B1-4 (High Five) cells engineered to coexpress the JE virus (JEV) premembrane (prM) and envelope (E) proteins. A higher yield of E protein was attained with recombinant High Five cells than with the baculovirus-insect cell system. This study demonstrated that recombinant insect cells offer a promising approach to the high-level production of VLPs for use as vaccines and diagnostic antigens.
authors: Department of Chemical Science and Engineering; Graduate School of Engineering; Kobe University; Kobe, Japan; BIKEN Endowed Department of Dengue Vaccine Development; Faculty of Tropical Medicine; Mahidol University; Bangkok, Thailand.
authors: Hideki
authors: H
authors: Yamaji
authors: BIKEN Endowed Department of Dengue Vaccine Development; Faculty of Tropical Medicine; Mahidol University; Bangkok, Thailand.
authors: Eiji
authors: E
authors: Konishi
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000502:physiology; D060846:Cell Engineering / Q000379:methods*; D004664:Encephalitis Virus, Japanese; D007313:Insecta / Q000166:cytology* / Q000821:virology*; D011994:Recombinant Proteins / Q000096:biosynthesis; D014759:Viral Envelope Proteins / Q000096:biosynthesis
chemical_list: D011994:Recombinant Proteins; D014759:Viral Envelope Proteins
keywords: High Five; Japanese encephalitis virus; insect cell; recombinant protein production; virus-like particle
references: Gene. 1991 Feb 15;98(2):177-83
references: 2016060
references: Biotechnol Prog. 1999 Nov-Dec;15(6):1046-52
references: 10585188
references: Trends Microbiol. 2003 Sep;11(9):438-44
references: 13678860
references: Vaccine. 2008 Aug 19;26 Suppl 10:K53-61
references: 18847557
references: Nat Rev Microbiol. 2005 Jan;3(1):13-22
references: 15608696
references: J Gen Virol. 2006 Oct;87(Pt 10):2755-2766
references: 16963734
references: J Virol. 2001 Mar;75(5):2204-12
references: 11160724
references: J Biosci Bioeng. 2012 Dec;114(6):657-62
references: 22841798
references: J Invertebr Pathol. 2011 Jul;107 Suppl:S42-8
references: 21784230
references: Science. 2008 Mar 28;319(5871):1834-7
references: 18369148
references: Science. 2008 Mar 28;319(5871):1830-4
references: 18369147
references: Appl Microbiol Biotechnol. 2013 Feb;97(3):1071-9
references: 22948954
references: Clin Vaccine Immunol. 2010 Oct;17(10):1560-6
references: 20668137
references: Vaccine. 2002 Jan 15;20(7-8):1058-67
references: 11803066
references: Biotechnol Bioeng. 1998 Dec 20;60(6):656-63
references: 10099475
references: Nat Biotechnol. 2005 May;23(5):567-75
references: 15877075
references: J Biosci Bioeng. 2010 Nov;110(5):577-81
references: 20591732
references: Methods. 2006 Sep;40(1):60-5
references: 16997714
references: Adv Virus Res. 2006;68:113-56
references: 16997011
references: J Invertebr Pathol. 2011 Jul;107 Suppl:S31-41
references: 21784229
references: Hum Vaccin. 2008 Jan-Feb;4(1):5-12
references: 18438104
references: Appl Microbiol Biotechnol. 2011 Feb;89(4):893-902
references: 21063703
references: J Biol Chem. 1997 Dec 5;272(49):30724-8
references: 9388209
references: Biotechnol Bioeng. 1999 Aug 20;64(4):426-33
references: 10397881
references: Virology. 1994 Nov 15;205(1):300-13
references: 7975226
references: Vaccine. 2012 Feb 27;30(10):1759-66
references: 22265860
references: Adv Virus Res. 2006;68:193-253
references: 16997013
references: Vaccine. 2012 Mar 16;30(13):2329-35
references: 22306856