title: Transmission of porcine circovirus type 2b (PCV2b) in Kunming mice.
abstract: To investigate porcine circovirus type 2b (PCV2b) transmission by contact and vertical infection in Kunming mice (an outbred mouse stock deriving from Swiss albino mice with a high ratio of gene heterozygosis), four mice in cage 6 were inoculated with PCV2b and 25 mice without any treatment were placed into cages 1 to 5 (five mice in each cage). Seven days after being infected, the PCV2-binoculated mice were co-mingled with non-inoculated mice from cages 1 to 5 successively at 7, 14, 21, 28 and 35 days post infection (dpi), respectively, for 3 days. In addition, eleven pregnant mice were injected with PCV2b. Samples were collected from non-inoculated mice and three newborn mice from each litter for PCV2b detection by polymerase chain reaction (PCR) and immunohistochemistry (IHC). The PCR results showed that PCV2b transmission rate among mice in cages 1, 2, 3, 4 and 5 was 0/5, 2/5, 5/5, 5/5 and 1/5, respectively. PCV2b antigen signals generally appeared in most organs of the non-inoculated mice in which viruses were detected by PCR. PCV2b DNA was also detected in newborn mice of PCV2b-infected litters, and viral antigen signals were observed in their organs as well. PCV2b was transmitted in Kunming mice by contact, and it also caused vertical infection through the placenta.
authors: Hunan Agricultural University College of Veterinary Medicine Changsha, Hunan 410128 China.
authors: Zhi Bang
authors: ZB
authors: Deng
authors: An Wen
authors: AW
authors: Yuan
authors: Wei
authors: W
authors: Luo
authors: Nai Dong
authors: ND
authors: Wang
authors: Qian Long
authors: QL
authors: Gong
authors: Xing Long
authors: XL
authors: Yu
authors: Li Qun
authors: LQ
authors: Xue
mesh_terms: D000818:Animals; D000956:Antigens, Viral; D018173:Circoviridae Infections / Q000821:virology; D017925:Circovirus / Q000276:immunology; D004279:DNA, Viral / Q000235:genetics; D051379:Mice; D016133:Polymerase Chain Reaction; D013553:Swine Diseases
chemical_list: D000956:Antigens, Viral; D004279:DNA, Viral