title: Blood meal-induced changes to antennal transcriptome profiles reveal shifts in odor sensitivities in Anopheles gambiae.
abstract: Olfactory-driven behaviors are central to the lifecycle of the malaria vector mosquito Anopheles gambiae and are initiated by peripheral signaling in the antenna and other olfactory tissues. To continue gaining insight into the relationship between gene expression and olfaction, we have performed cohort comparisons of antennal transcript abundances at five time points after a blood meal, a key event in both reproduction and disease transmission cycles. We found that more than 5,000 transcripts displayed significant abundance differences, many of which were correlated by cluster analysis. Within the chemosensory gene families, we observed a general reduction in the level of chemosensory gene transcripts, although a subset of odorant receptors (AgOrs) was modestly enhanced in post-blood-fed samples. Integration of AgOr transcript abundance data with previously characterized AgOr excitatory odorant response profiles revealed potential changes in antennal odorant receptivity that coincided with the shift from host-seeking to oviposition behaviors in blood-fed female mosquitoes. Behavioral testing of ovipositing females to odorants highlighted by this synthetic analysis identified two unique, unitary oviposition cues for An. gambiae, 2-propylphenol and 4-methylcyclohexanol. We posit that modest, yet cumulative, alterations of AgOr transcript levels modulate peripheral odor coding resulting in biologically relevant behavioral effects. Moreover, these results demonstrate that highly quantitative, RNAseq transcript abundance data can be successfully integrated with functional data to generate testable hypotheses.
authors: Center for Human Genetics Research, Vanderbilt University Medical Center, Nashville, TN 37235, USA.
authors: David C
authors: DC
authors: Rinker
authors: R Jason
authors: RJ
authors: Pitts
authors: Xiaofan
authors: X
authors: Zhou
authors: Eunho
authors: E
authors: Suh
authors: Antonis
authors: A
authors: Rokas
authors: Laurence J
authors: LJ
authors: Zwiebel
mesh_terms: D000818:Animals; D000852:Anopheles / Q000378:metabolism / Q000502:physiology*; D057506:Arthropod Antennae / Q000378:metabolism*; D001769:Blood; D016000:Cluster Analysis; D019295:Computational Biology; D005260:Female; D005786:Gene Expression Regulation; D009812:Odorants; D012313:RNA / Q000378:metabolism; D018035:Receptors, Odorant / Q000235:genetics / Q000378:metabolism*; D017422:Sequence Analysis, DNA; D014158:Transcription, Genetic; D059467:Transcriptome
chemical_list: D018035:Receptors, Odorant; D012313:RNA
keywords: bioinformatics; chemoreceptor; transcriptomics
references: J Am Mosq Control Assoc. 1989 Sep;5(3):311-6
references: 2573687
references: Chem Senses. 1998 Jun;23(3):351-7
references: 9669048
references: Trends Parasitol. 2012 Mar;28(3):114-21
references: 22300806
references: Curr Biol. 2004 Apr 20;14(8):638-49
references: 15084278
references: J Insect Physiol. 2001 Mar;47(3):303-310
references: 11119776
references: BMC Mol Biol. 2006 Oct 27;7:38
references: 17069657
references: Genes Dev. 2012 Nov 15;26(22):2483-98
references: 23105004
references: Proc Natl Acad Sci U S A. 2004 Apr 6;101(14):5058-63
references: 15037749
references: Science. 1997 Nov 28;278(5343):1632-5
references: 9374465
references: PLoS Genet. 2010 Aug 19;6(8):e1001064
references: 20808886
references: Curr Biol. 2007 Sep 18;17(18):1533-44
references: 17764944
references: Proc Natl Acad Sci U S A. 2006 Sep 5;103(36):13526-31
references: 16938890
references: J Med Entomol. 2003 Sep;40(5):653-6
references: 14596278
references: Science. 2011 Mar 11;331(6022):1333-6
references: 21393546
references: J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 2005 Sep;191(9):777-90
references: 16094545
references: Insect Biochem Mol Biol. 2005 Jul;35(7):661-75
references: 15894184
references: J Neurosci. 2008 Apr 30;28(18):4745-55
references: 18448651
references: J Med Entomol. 1994 Jul;31(4):618-22
references: 7932610
references: Dev Biol. 2006 May 1;293(1):127-41
references: 16515779
references: Nature. 2010 Mar 4;464(7285):66-71
references: 20130575
references: J Insect Physiol. 2010 Jun;56(6):659-65
references: 20153749
references: Cell. 2009 Jan 9;136(1):149-62
references: 19135896
references: Nature. 1999 Jul 22;400(6742):375-8
references: 10432117
references: Cell. 2012 Aug 31;150(5):1042-54
references: 22939627
references: BMC Genomics. 2011 May 27;12:271
references: 21619637
references: Proc Natl Acad Sci U S A. 2008 Apr 29;105(17):6433-8
references: 18427108
references: Med Vet Entomol. 1996 Jul;10(3):269-76
references: 8887339
references: Front Physiol. 2013 Mar 07;4:39
references: 23471139
references: Neuron. 2005 Mar 3;45(5):661-6
references: 15748842
references: Proc Natl Acad Sci U S A. 2001 Dec 4;98(25):14693-7
references: 11724964
references: PLoS Biol. 2010 Aug 31;8(8):
references: 20824161
references: PLoS One. 2008 Aug 22;3(8):e3045
references: 18725946
references: Proc Natl Acad Sci U S A. 2010 Mar 2;107(9):4418-23
references: 20160092
references: Insect Mol Biol. 2003 Dec;12(6):549-60
references: 14986916
references: FEBS Lett. 2006 Jan 9;580(1):2-8
references: 16337945
references: Insect Mol Biol. 2005 Aug;14(4):365-73
references: 16033430
references: Science. 2002 Oct 4;298(5591):176-8
references: 12364795
references: J Chem Ecol. 2010 Mar;36(3):245-8
references: 20191395
references: Annu Rev Entomol. 2013;58:373-91
references: 23020622
references: Chem Senses. 2006 Nov;31(9):845-63
references: 16963500
references: Annu Rev Entomol. 1999;44:131-57
references: 9990718
references: Biochem Biophys Res Commun. 2003 Dec 19;312(3):545-54
references: 14680800
references: Curr Biol. 2008 Jun 3;18(11):803-7
references: 18499459
references: Insect Biochem Mol Biol. 2004 Jul;34(7):645-52
references: 15242705
references: J Biol Rhythms. 2001 Jun;16(3):205-15
references: 11407780
references: Insect Mol Biol. 2006 Feb;15(1):1-12
references: 16469063
references: Curr Opin Neurobiol. 2009 Aug;19(4):345-53
references: 19660932
references: Proc Natl Acad Sci U S A. 2011 Aug 9;108(32):E421-30
references: 21715657
references: Med Vet Entomol. 2004 Dec;18(4):429-38
references: 15642010