title: [Improved lipid productivity of Nannochloropsis by heavy-ion irradiation mutagenesis].
abstract: Nannochloropsis has been considered as a promising feedstock for biodiesel production in recent years. To improve its lipid productivity, heavy-ion irradiation mutagenesis, an effectively breeding method used in plants and microorganisms was applied in Nannochloropsis oceanica OZ-1. After large-scale screening using Imaging-PAM and microplate-reader, two mutants (HP-1 and HP-2) with higher growth rate were isolated from the wild type N. oceanica. Subsequently analysis showed that after 18 days of cultivation biomass accumulation of the HP-1 and HP-2 mutant was increased by 18% and 26% respectively compare to the wild type. Total lipid productivity of the HP-1 and HP-2 mutant was 295 mg/(L x d) and 275 mg/(L x d), respectively, whereas that of the wild type was 247 mg/(L x d). Both mutants showed significantly advantage over their wild type concerning biomass accumulation and lipid productivity.
authors: Department of Biotechnology, School of Life Science and Technology, Jinan University, Guangzhou 510632, Guangdong, China.
authors: Zhiyao
authors: Z
authors: Wang
authors: Yubin
authors: Y
authors: Ma
authors: Runzhi
authors: R
authors: Mu
authors: Changjiang
authors: C
authors: Sun
authors: Dongyuan
authors: D
authors: Zhang
authors: Yongfei
authors: Y
authors: Wang
mesh_terms: D020450:Heavy Ions; D008055:Lipids / Q000096:biosynthesis*; D058086:Microalgae / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects; D016296:Mutagenesis / Q000235:genetics*; D009154:Mutation / Q000235:genetics; D058009:Stramenopiles / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects
chemical_list: D008055:Lipids