title: Synchrotron radiation X-ray phase micro-computed tomography as a new method to detect iron oxide nanoparticles in the brain.
abstract: OBJECTIVE
  The purpose of this study was to introduce synchrotron radiation X-ray phase computed tomography (SR-PCT) as a new method of visualizing ultrasmall superparamagnetic particles of iron oxide (USPIO) distribution into the brains of mice with neuroinflammation.
  
  
  METHODS
  The sensitivity of the technique was assessed by performing back-to-back SR-PCT and magnetic resonance imaging (MRI) in mice stereotaxically injected with a range of USPIO concentrations. Eight mice with cerebral ischemia were then intravenously injected with USPIOs and imaged back-to-back with MRI and SR-PCT.
  
  
  RESULTS
  SR-PCT proved sensitive enough to detect iron in nanomolar quantities. In stroke-induced animals, SR-PCT showed hyperintense areas in the regions of MR signal loss and immunostaining for macrophages. SR-PCT, moreover, identified brain anatomy as clearly as histology, without the need for sectioning or staining, with an examination time of 44 min per brain at an isotropic spatial resolution of 8 μm.
  
  
  CONCLUSIONS
  SR-PCT has potential for cellular imaging in intact brain, with unequaled neuroanatomy.
authors: Université de Lyon, Lyon 1, France.
authors: M
authors: M
authors: Marinescu
authors: M
authors: M
authors: Langer
authors: A
authors: A
authors: Durand
authors: C
authors: C
authors: Olivier
authors: A
authors: A
authors: Chabrol
authors: H
authors: H
authors: Rositi
authors: F
authors: F
authors: Chauveau
authors: T H
authors: TH
authors: Cho
authors: N
authors: N
authors: Nighoghossian
authors: Y
authors: Y
authors: Berthezène
authors: F
authors: F
authors: Peyrin
authors: M
authors: M
authors: Wiart
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D002545:Brain Ischemia / Q000175:diagnosis / Q000473:pathology; D003911:Dextrans / Q000378:metabolism*; D004195:Disease Models, Animal; D007091:Image Processing, Computer-Assisted; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles; D008297:Male; D051379:Mice; D019047:Phantoms, Imaging; D012031:Refractometry; D013238:Stereotaxic Techniques; D017356:Synchrotrons; D055114:X-Ray Microtomography / Q000379:methods*
chemical_list: D003911:Dextrans; D058185:Magnetite Nanoparticles; C097921:ferumoxtran-10
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