title: A preparation of cow's late colostrum fraction containing αs1-casein promoted the proliferation of cultured rat intestinal IEC-6 epithelial cells.
abstract: Colostrum is a complex mixture of bioactives that promotes neonate growth. Recently, we have found by in vivo study that skimmed, sterilized, and concentrated bovine late colostrum (SCBLC), obtained from a Holstein herd on days 6-7 after parturition, had an ability to maintain intestinal integrity. In the present study we investigated effects of SCBLC on rat intestinal IEC-6 cell proliferation in vitro. A fraction containing αs1-casein was found to have a robust stimulation effect as compared to other protein fractions from SCBLC and even the αs1-casein fraction from milk from other Holstein herds. Furthermore, the SCBLC αs1-casein molecule demonstrated not only slightly slower mobility on both SDS- and native-PAGE than other bovine milk αs1-caseins, but also a peculiar conformation reminiscent of moltenglobule in the circular dichroism spectrum. These findings may be of relevant to the competence of SCBLC to preserve intestinal integrity.
authors: United Graduate School of Agricultural Science, Gifu University, Yanagido, Gifu, Gifu, Japan.
authors: Cairangzhuoma
authors: Mayumi
authors: M
authors: Yamamoto
authors: Xijier
authors: Mizuho
authors: M
authors: Inagaki
authors: Kenji
authors: K
authors: Uchida
authors: Kousaku
authors: K
authors: Yamashita
authors: Shouichiro
authors: S
authors: Saito
authors: Tomio
authors: T
authors: Yabe
authors: Yoshihiro
authors: Y
authors: Kanamaru
mesh_terms: D000818:Animals; D002364:Caseins / Q000302:isolation & purification* / Q000494:pharmacology*; D002417:Cattle; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003126:Colostrum / Q000737:chemistry*; D005260:Female; D007413:Intestinal Mucosa / Q000166:cytology*; D008892:Milk / Q000737:chemistry; D008894:Milk Proteins / Q000494:pharmacology; D011247:Pregnancy; D051381:Rats; D013045:Species Specificity; D000067816:Whey Proteins
chemical_list: D002364:Caseins; D008894:Milk Proteins; D000067816:Whey Proteins