title: Primary hippocampal neurons, which lack four crucial extracellular matrix molecules, display abnormalities of synaptic structure and function and severe deficits in perineuronal net formation.
abstract: The extracellular matrix (ECM) of the brain plays crucial roles during the development, maturation, and regeneration of the CNS. In a subpopulation of neurons, the ECM condenses to superstructures called perineuronal nets (PNNs) that surround synapses. Camillo Golgi described PNNs a century ago, yet their biological functions remain elusive. Here, we studied a mouse mutant that lacks four ECM components highly enriched in the developing brain: the glycoproteins tenascin-C and tenascin-R and the chondroitin sulfate proteoglycans brevican and neurocan. Primary embryonic hippocampal neurons and astrocytes were cultivated using a cell insert system that allows for co-culture of distinct cell populations in the absence of direct membrane contacts. The wild-type and knock-out cells were combined in the four possible permutations. Using this approach, neurons cultivated in the presence of mutant astrocytes displayed a transient increase of synapses after 2 weeks. However, after a period of 3 weeks or longer, synapse formation and stabilization were compromised when either neuron or astrocyte cell populations or both were of mutant origin. The development of PNN structures was observed, but their size was substantially reduced on knock-out neurons. The synaptic activity of both wild-type and knock-out neurons was monitored using whole-cell patch clamping. The salient observation was a reduced frequency of IPSCs and EPSCs, whereas the amplitudes were not modified. Remarkably, the knock-out neuron phenotypes could not be rescued by wild-type astrocytes. We conclude that the elimination of four ECM genes compromises neuronal function.
authors: Department of Cell Morphology and Molecular Neurobiology, Ruhr-University, D-44780 Bochum, Germany.
authors: Maren
authors: M
authors: Geissler
authors: Christine
authors: C
authors: Gottschling
authors: Ainhara
authors: A
authors: Aguado
authors: Uwe
authors: U
authors: Rauch
authors: Christian H
authors: CH
authors: Wetzel
authors: Hanns
authors: H
authors: Hatt
authors: Andreas
authors: A
authors: Faissner
mesh_terms: D000818:Animals; D001253:Astrocytes; D058581:Brevican / Q000172:deficiency; D002452:Cell Count; D002478:Cells, Cultured; D018920:Coculture Techniques; D004622:Embryo, Mammalian; D019706:Excitatory Postsynaptic Potentials / Q000235:genetics / Q000502:physiology; D016326:Extracellular Matrix Proteins / Q000145:classification / Q000172:deficiency*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D006624:Hippocampus / Q000166:cytology*; D053444:Inhibitory Postsynaptic Potentials / Q000235:genetics / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009415:Nerve Net / Q000473:pathology* / Q000502:physiology; D058580:Neurocan / Q000172:deficiency; D009474:Neurons / Q000502:physiology*; D013569:Synapses / Q000235:genetics* / Q000502:physiology; D019063:Tenascin / Q000172:deficiency
chemical_list: C553057:Bcan protein, mouse; D058581:Brevican; D016326:Extracellular Matrix Proteins; D058580:Neurocan; D019063:Tenascin; C074638:tenascin R
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