title: RNA localization in Xenopus oocytes uses a core group of trans-acting factors irrespective of destination.
abstract: The 3' untranslated region of mRNA encoding PHAX, a phosphoprotein required for nuclear export of U-type snRNAs, contains cis-acting sequence motifs E2 and VM1 that are required for localization of RNAs to the vegetal hemisphere of Xenopus oocytes. However, we have found that PHAX mRNA is transported to the opposite, animal, hemisphere. A set of proteins that cross-link to the localization elements of vegetally localized RNAs are also cross-linked to PHAX and An1 mRNAs, demonstrating that the composition of RNP complexes that form on these localization elements is highly conserved irrespective of the final destination of the RNA. The ability of RNAs to bind this core group of proteins is correlated with localization activity. Staufen1, which binds to Vg1 and VegT mRNAs, is not associated with RNAs localized to the animal hemisphere and may determine, at least in part, the direction of RNA movement in Xenopus oocytes.
authors: Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana 46556, USA.
authors: Donald D
authors: DD
authors: Snedden
authors: Michelle M
authors: MM
authors: Bertke
authors: Dominic
authors: D
authors: Vernon
authors: Paul W
authors: PW
authors: Huber
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D001483:Base Sequence; D016297:Mutagenesis, Site-Directed; D009865:Oocytes / Q000166:cytology*; D010750:Phosphoproteins / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding; D034443:RNA Transport; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D012261:Ribonucleoproteins / Q000235:genetics / Q000378:metabolism; D020825:T-Box Domain Proteins / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics*; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism; D014981:Xenopus / Q000235:genetics*; D029867:Xenopus Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; C513103:GDF1 protein, Xenopus; D010750:Phosphoproteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D012261:Ribonucleoproteins; C488764:STAU1 protein, Xenopus; D020825:T-Box Domain Proteins; D015534:Trans-Activators; D016212:Transforming Growth Factor beta; C103966:VegT protein, Xenopus; D029867:Xenopus Proteins
keywords: PHAX; RNA localization; Staufen; Vg1 mRNA; Xenopus oocyte
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