title: The integration of HR-HPV increases the expression of cyclins A and E in cytologies with and without low-grade lesions.
abstract: BACKGROUND
  Cyclin-A and cyclin-E are regulators of G1-S phase of normal cell cycle. Integration of human papilloma virus high-risk (HR-HPV) could alter this mechanism, and its overexpression has been associated with poor prognosis in cervical cancer.
  
  
  OBJECTIVE
  To determine the expression of cyclin-A and cyclin-E, types of HR-HPV and physical state of DNA in cytologies with the diagnosis of low-grade squamous intraepithelial lesion (LSIL).
  
  
  METHODS
  115 cytological specimens in liquid base (liquid-PREP(™)) were analyzed. 25 specimens were with no signs of SIL (NSIL) and without HPV; 30 with NSIL with low-risk HPV (LR-HPV); 30 with NSIL with HR-HPV; and 30 with both LSIL and HR-HPV. The expression of cyclins was evaluated by immunocytochemistry; and the detection of viral DNA was done by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLPs) for genotyping or sequencing of HPV. The physical state of HPV was evaluated by in situ hybridization with amplification with tyramide.
  
  
  RESULTS
  In the cytologies NSIL with LR-HPV, the expression of cyclin-A and cyclin-E was found respectively in 23.3% and 33.3% of the specimens. Among the specimens of NSIL with HR-HPV, 33.3% expressed cyclin-A and 40% cyclin-E, while 100% of the LSILs expressed the 2 cyclins. On the other hand, 100% of the samples NSIL with LR-HPV presented an episomal pattern. Of the specimens of NSIL with HR-HPV, 56.6% exhibited an episomal pattern, 23.3% integrated and 20%, mixed. Among the LSILs, 90% were mixed and 10% integrated.
  
  
  CONCLUSIONS
  The cyclins A and E are present in the LSILs that occur predominantly in mixed state in the presence of HR-HPV.
authors: Laboratory for Research in Cytopathology and Histoquemical, Academic Unit for Chemical Biological Sciences, Autonomous University of Guerrero, Chilpancingo, Guerrero, Mexico.
authors: M I
authors: MI
authors: Zubillaga-Guerrero
authors: B
authors: B
authors: Illades-Aguiar
authors: M A
authors: MA
authors: Leyva-Vazquez
authors: E
authors: E
authors: Flores-Alfaro
authors: E
authors: E
authors: Castañeda-Saucedo
authors: J F
authors: JF
authors: Muñoz-Valle
authors: L C
authors: LC
authors: Alarcón-Romero
keywords: Cyclin-A; cyclin-E; high-risk human papillomavirus; in situ hybridization; low-grade squamous intraepithelial lesion
references: Histol Histopathol. 2007 Jun;22(6):587-92
references: 17357088
references: Cancer. 2008 Dec 25;114(6):474-80
references: 19016301
references: Gynecol Oncol. 2010 May;117(2):291-6
references: 20199804
references: Am J Surg Pathol. 2001 Jul;25(7):884-91
references: 11420459
references: J Infect Dis. 1994 Nov;170(5):1077-85
references: 7963696
references: Zhonghua Yi Xue Za Zhi. 2011 Apr 5;91(13):906-10
references: 21600119
references: BMC Cancer. 2006 Mar 08;6:55
references: 16524460
references: Best Pract Res Clin Obstet Gynaecol. 2005 Aug;19(4):469-83
references: 16150388
references: Virology. 2008 Oct 10;380(1):21-5
references: 18718623
references: Mod Pathol. 1998 Dec;11(12):1238-46
references: 9872657
references: Cancer. 1998 May 1;82(9):1709-19
references: 9576293
references: Br J Cancer. 2006 Apr 10;94(7):1045-50
references: 16538218
references: J Clin Virol. 2005 Mar;32 Suppl 1:S7-15
references: 15753007
references: Cancer Res. 2004 Jun 1;64(11):3878-84
references: 15172997
references: J Clin Microbiol. 2002 Mar;40(3):886-91
references: 11880410
references: J Clin Pathol. 2006 May;59(5):513-7
references: 16484445
references: J Clin Microbiol. 2008 Nov;46(11):3678-85
references: 18799702
references: N Engl J Med. 2003 Feb 6;348(6):518-27
references: 12571259
references: Mod Pathol. 2002 Dec;15(12):1339-47
references: 12481016
references: Cancer Epidemiol Biomarkers Prev. 2008 Apr;17(4):810-7
references: 18398022
references: J Clin Pathol. 2003 Jul;56(7):532-6
references: 12835300
references: Diagn Cytopathol. 2002 Dec;27(6):365-70
references: 12451568
references: Int J Cancer. 2006 Mar 1;118(5):1254-60
references: 16161049
references: JAMA. 2002 Apr 24;287(16):2114-9
references: 11966386
references: Gynecol Oncol. 1999 May;73(2):223-8
references: 10329038
references: J Clin Virol. 2009 Jun;45(2):96-9
references: 19398216
references: Virchows Arch. 2005 Jun;446(6):626-33
references: 15891905
references: Adv Anat Pathol. 2006 Jul;13(4):190-4
references: 16858153