title: Influence of matrix metalloproteinase-1 gene -1607 (1G/2G) (rs1799750) promoter polymorphism on circulating levels of MMP-1 in chronic pancreatitis.
abstract: This study investigated the role of -1607 (1G/2G) (rs1799750) polymorphism of the MMP-1 gene in chronic pancreatitis. We genotyped 100 patients with chronic pancreatitis and 100 control subjects using tetra-primer ARMS-PCR followed by agarose gel electrophoresis. Serum levels of MMP-1 were determined by Elisa. Statistical analysis was applied to test the significance of the results. The genotypic and allelic distribution varied significantly between the disease group and the control subjects [OD = 1.981 (1.236-3.181), p = 0.004]. MMP-1 levels were higher in subjects homozygous for the 2G allele than in subjects with the 1G allele. The present study revealed a significant association of the MMP-1 -1607 1G/2G (rs1799750) gene promoter polymorphism with chronic pancreatitis, and it can be considered a biological marker in the etiology of chronic pancreatitis.
authors: Institute of Genetics and Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad 500 016, India.
authors: K
authors: K
authors: Sri Manjari
authors: Pratibha
authors: P
authors: Nallari
authors: N
authors: N
authors: Balakrishna
authors: A
authors: A
authors: Vidyasagar
authors: B
authors: B
authors: Prabhakar
authors: A
authors: A
authors: Jyothy
authors: A
authors: A
authors: Venkateshwari
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D005838:Genotype; D006720:Homozygote; D006801:Humans; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000235:genetics*; D008875:Middle Aged; D010195:Pancreatitis / Q000201:enzymology / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D055815:Young Adult
chemical_list: C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1