title: Recent developments in the field of the determination of constituents of TCMs in body fluids of animals and human.
abstract: Although traditional Chinese medicines (TCMs) play important role in drug discovery and human health, the actual value of TCMs has not been fully recognized worldwide due to its complex components and uncontrollable quality. For the modernization and globalization of TCMs, it is important to establish selective, sensitive and feasible analytical methods for determination and quantification of bioactive components of TCMs in body fluids primarily due to the low concentration, the complex nature of the biological matrices, and multi-components and their metabolites present in biological fluids. The present review summarizes the current extraction techniques, chromatographic separation and spectroscopic (especially mass spectrometric) analysis methods and new trends on the analysis of bioactive components and metabolites of TCMs in biological fluids. In addition, the importance of establishment of pharmacokinetics and bioavailability profiles and simultaneous determination of multi-active components in TCMs is discussed to provide proper examples of analytical methods for pharmacological and clinical studies of TCMs.
authors: Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai 200433, China; Shanghai Key Laboratory for Pharmaceutical Metabolite Research, No. 325 Guohe Road, Shanghai 200433, China. Electronic address: fygao@outlook.com.
authors: Fangyuan
authors: F
authors: Gao
authors: Yongsheng
authors: Y
authors: Hu
authors: Gao
authors: G
authors: Fang
authors: Guang
authors: G
authors: Yang
authors: Zihua
authors: Z
authors: Xu
authors: Lili
authors: L
authors: Dou
authors: Zhao
authors: Z
authors: Chen
authors: Guorong
authors: G
authors: Fan
mesh_terms: D000818:Animals; D001682:Biological Availability; D001826:Body Fluids / Q000737:chemistry; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D055808:Drug Discovery; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry / Q000493:pharmacokinetics; D006801:Humans; D008516:Medicine, Chinese Traditional / Q000592:standards*; D011786:Quality Control
chemical_list: D004365:Drugs, Chinese Herbal
keywords: Active constituent; Body fluid; Metabolite; Pharmacokinetics; Traditional Chinese medicine