title: Stereoselective accumulation of propranolol enantiomers in K562 and K562/ADR cells.
abstract: The stereoselective uptake of propranolol enantiomers was investigated by using the K562 and K562 adriamycin-resistant cell line (K562/ADR) as a model. An enantioselective RP-HPLC method was applied to determine the accumulation of propranolol (PPL) stereoisomers in K562 and K562/ADR cells. The concentration, time and temperature dependent studies showed that the accumulation of S-(-)-PPL was higher than R-(+)-PPL in K562 cells and uptake of R-(+)-PPL was significantly higher than that of S-(-)-PPL in K562/ADR cells. The results indicate the enantioselective accumulation of propranolol enantiomers in K562 and K562 / ADR cells.
authors: Laboratory of Pharmaceutical Analysis and Drug Metabolism, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China.
authors: Xiao-Li
authors: XL
authors: Zheng
authors: Qin-Qin
authors: QQ
authors: Yu
authors: Yi
authors: Y
authors: Wang
authors: Su
authors: S
authors: Zeng
mesh_terms: D002851:Chromatography, High Pressure Liquid; D006801:Humans; D020014:K562 Cells; D015394:Molecular Structure; D011433:Propranolol / Q000737:chemistry* / Q000378:metabolism*; D013237:Stereoisomerism
chemical_list: D011433:Propranolol