title: Antioxidant effect of human adult adipose-derived stromal stem cells in alveolar epithelial cells undergoing stretch.
abstract: BACKGROUND
  Alveolar epithelial cells undergo stretching during mechanical ventilation. Stretch can modify the oxidative balance in the alveolar epithelium. The aim of the present study was to evaluate the antioxidant role of human adult adipose tissue-derived stromal cells (hADSCs) when human alveolar epithelial cells were subjected to injurious cyclic overstretching.
  
  
  METHODS
  A549 cells were subjected to biaxial stretch (0-15% change in surface area for 24h, 0.2Hz) with and without hADSCs. At the end of the experiments, oxidative stress was measured as superoxide generation using positive nuclear dihydroethidium (DHE) staining, superoxide dismutase (SOD) activity in cell lysates, 8-isoprostane concentrations in supernatant, and 3-nitrotyrosine by indirect immunofluorescence in fixed cells.
  
  
  RESULTS
  Cyclically stretching of AECs induced a significant decrease in SOD activity, and an increase in 8-isoprostane concentrations, DHE staining and 3-nitrotyrosine staining compared with non-stretched cells. Treatment with hADSCs significantly attenuated stretch-induced changes in SOD activity, 8-isoprostane concentrations, DHE and 3-nitrotyrosine staining.
  
  
  CONCLUSIONS
  These data suggest that hADSCs have an anti-oxidative effect in human alveolar epithelial cells undergoing cyclic stretch.
authors: Critical Care Department, Hospital Universitario de Getafe, Madrid, Spain. openuelas@gmail.com
authors: Oscar
authors: O
authors: Peñuelas
authors: Esther
authors: E
authors: Melo
authors: Carolina
authors: C
authors: Sánchez
authors: Isabel
authors: I
authors: Sánchez
authors: Kieran
authors: K
authors: Quinn
authors: Antonio
authors: A
authors: Ferruelo
authors: Francisco
authors: F
authors: Pérez-Vizcaíno
authors: Andrés
authors: A
authors: Esteban
authors: Daniel
authors: D
authors: Navajas
authors: Nicolás
authors: N
authors: Nin
authors: José A
authors: JA
authors: Lorente
authors: Ramón
authors: R
authors: Farré
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D000328:Adult; D053687:Adult Stem Cells / Q000378:metabolism*; D002478:Cells, Cultured; D006801:Humans; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology*; D011650:Pulmonary Alveoli / Q000166:cytology / Q000378:metabolism*; D020545:Respiratory Mucosa / Q000166:cytology / Q000378:metabolism*; D013314:Stress, Mechanical; D017154:Stromal Cells / Q000378:metabolism; D055815:Young Adult