title: Candida albicans susceptibility to lactoperoxidase-generated hypoiodite.
abstract: In vivo, lactoperoxidase produces hypothiocyanite (OSCN(-)) from thiocyanate (SCN(-)) in the presence of hydrogen peroxide (H2O2); in vitro, iodide (I(-)) can be oxidized into hypoiodite (OI(-)) by this enzyme. The aim of this study was to compare in vitro the anti-Candida effect of iodide versus thiocyanate used as lactoperoxidase substrate to prevent Candida biofilms development. Candida albicans ATCC 10231 susceptibility upon both peroxidase systems was tested in three different experimental designs: (i) in a liquid culture medium, (ii) in an interface model between solid culture medium and gel containing the enzymic systems, (iii) in a biofilm model onto titanium and acrylic resin. Yeast growth in liquid medium was monitored by turbidimetry at 600 nm. Material-adherent yeast biomass was evaluated by the tetrazolium salt MTT method. The iodide-peroxidase system has been shown to inhibit Candida biofilm formation at lower substrate concentrations (~200 fold less H2O2 donor) and for longer incubation periods than the thiocyanate-peroxidase system. In conclusion, efficiency of lactoperoxidase-generated OI(-) to prevent C. albicans biofilm development allows refining iodine antifungal use in ex vivo conditions.
authors: Laboratory of Experimental Hormonology, Université Libre de Bruxelles, Brussels, Belgium.
authors: Mohamed
authors: M
authors: Ahariz
authors: Philippe
authors: P
authors: Courtois
keywords: denture; iodide; oral; peroxidase; saliva; titanium
references: Gerodontology. 2008 Dec;25(4):222-8
references: 18665849
references: Biochemistry. 1988 Jan 12;27(1):233-9
references: 3349028
references: Int J Antimicrob Agents. 2008 Mar;31(3):272-6
references: 18242063
references: Am J Pediatr Hematol Oncol. 1985 Spring;7(1):82-6
references: 3898901
references: J Infect Dis. 1985 Nov;152(5):938-45
references: 2413145
references: Infect Immun. 1983 Apr;40(1):97-102
references: 6299966
references: J Pharm Sci. 2007 Nov;96(11):3144-50
references: 17724656
references: J Dent Res. 2008 Jun;87(6):594-8
references: 18502972
references: Clin Sci (Lond). 2004 Sep;107(3):281-9
references: 15128285
references: Microbios. 1989;57(231):73-83
references: 2661970
references: Indian J Pathol Microbiol. 2005 Jan;48(1):43-4
references: 16758789
references: J Biomed Mater Res. 2000 Dec 5;52(3):567-71
references: 11007626
references: J Bacteriol. 1969 Aug;99(2):361-5
references: 5817553
references: Bull Tokyo Dent Coll. 1998 Feb;39(1):7-14
references: 9663026
references: Rev Stomatol Chir Maxillofac. 2010 Apr;111(2):74-8
references: 20347465
references: Appl Environ Microbiol. 2003 Aug;69(8):4611-7
references: 12902249
references: Rev Infect Dis. 1980 Jul-Aug;2(4):625-32
references: 6255542
references: J Clin Pharm Ther. 1987 Dec;12(6):393-9
references: 3326886
references: J Appl Microbiol. 2000 Aug;89(2):215-24
references: 10971753
references: J Periodontol. 2005 Nov;76(11 Suppl):2154-60
references: 16277588
references: J Biol Buccale. 1992 Dec;20(4):241-5
references: 1306188
references: J Craniomaxillofac Surg. 2009 Oct;37(7):392-7
references: 19473851