title: Neuromuscular damage and repair after dry needling in mice.
abstract: Objective. Some dry needling treatments involve repetitive and rapid needle insertions into myofascial trigger points. This type of treatment causes muscle injury and can also damage nerve fibers. The aim of this study is to determine the injury caused by 15 repetitive punctures in the muscle and the intramuscular nerves in healthy mouse muscle and its ulterior regeneration. Methods. We repeatedly needled the levator auris longus muscle of mice, and then the muscles were processed with immunohistochemistry, methylene blue, and electron microscopy techniques. Results. Three hours after the dry needling procedure, the muscle fibers showed some signs of an inflammatory response, which progressed to greater intensity 24 hours after the procedure. Some inflammatory cells could still be seen when the muscle regeneration was almost complete seven days after the treatment. One day after the treatment, some changes in the distribution of receptors could be observed in the denervated postsynaptic component. Reinnervation was complete by the third day after the dry needling procedure. We also saw very fine axonal branches reinnervating all the postsynaptic components and some residual sprouts the same day. Conclusion. Repeated dry needling punctures in muscle do not perturb the different stages of muscle regeneration and reinnervation.
authors: Unit of Histology and Neurobiology, Department of Basic Medical Sciences, Faculty of Medicine and Health Sciences, Rovira i Virgili University, Carrer St. Llorenç No. 21, 43201 Reus, Spain.
authors: Ares
authors: A
authors: Domingo
authors: Orlando
authors: O
authors: Mayoral
authors: Sonia
authors: S
authors: Monterde
authors: Manel M
authors: MM
authors: Santafé
references: J Neurosci. 1990 Dec;10(12):3905-15
references: 2269890
references: Fed Proc. 1986 Apr;45(5):1456-60
references: 2420649
references: J Appl Physiol (1985). 2005 Nov;99(5):1977-84
references: 16037403
references: Arch Ophthalmol. 1985 Sep;103(9):1373-7
references: 4038130
references: Nat Neurosci. 2002 Sep;5(9):861-7
references: 12172551
references: Neurosci Res Program Bull. 1973 Jun;11(3):227-31
references: 4736778
references: J Anat. 1962 Apr;96:137-52
references: 13860510
references: Brain. 2008 Oct;131(Pt 10):2620-31
references: 18718965
references: J Gen Physiol. 1973 Jan;61(1):1-23
references: 4683095
references: Tissue Cell. 1976;8(4):673-92
references: 1020021
references: Cell Tissue Res. 2012 Mar;347(3):759-74
references: 21667167
references: J Appl Physiol (1985). 1985 Jul;59(1):119-26
references: 4030553
references: Proc R Soc Lond B Biol Sci. 1968 Feb 27;169(1016):289-306
references: 4384567
references: Brain. 2004 Mar;127(Pt 3):671-9
references: 14761904
references: Med Sci Sports Exerc. 1992 May;24(5):512-20
references: 1569847
references: Eur J Neurosci. 1999 May;11(5):1711-22
references: 10215925
references: J Neurocytol. 1975 Apr;4(2):141-55
references: 1123653
references: J Appl Physiol Respir Environ Exerc Physiol. 1983 Jan;54(1):80-93
references: 6826426
references: Lab Invest. 1995 Mar;72(3):341-7
references: 7898053
references: J Appl Physiol (1985). 2003 Jun;94(6):2494-501
references: 12576409
references: J Biophys Biochem Cytol. 1961 Feb;9:493-5
references: 13768451
references: J Bodyw Mov Ther. 2008 Oct;12(4):371-384
references: 19083696
references: J Neurosci. 1988 May;8(5):1759-66
references: 2966853
references: J Neurosci. 1989 May;9(5):1781-805
references: 2542480
references: J Electromyogr Kinesiol. 2004 Feb;14(1):95-107
references: 14759755
references: J Cell Biol. 2004 Aug 2;166(3):347-57
references: 15277541
references: Sports Med. 1989 Apr;7(4):207-34
references: 2657962
references: Phys Ther. 1982 Sep;62(9):1275-82
references: 7051055
references: Toxicol Appl Pharmacol. 1990 Sep 15;105(3):422-33
references: 2237917
references: Physiol Rev. 2004 Jan;84(1):209-38
references: 14715915
references: J Man Manip Ther. 2019 Jul;27(3):162-171
references: 30935326
references: J Anat. 1970 Nov;107(Pt 3):547-56
references: 5492943
references: J Neurosci. 1995 Oct;15(10):6445-52
references: 7472407
references: J Altern Complement Med. 2011 Aug;17(8):755-62
references: 21745098
references: Trends Mol Med. 2010 Apr;16(4):160-70
references: 20207196
references: Mol Ther. 2007 May;15(5):867-77
references: 17387336