title: Caveolae-mediated endocytosis of conjugated polymer nanoparticles.
abstract: Understanding the cellular entry pathways of synthetic biomaterials is highly important to improve overall labeling and delivery efficiency. Herein, cellular entry mechanisms of conjugated polymer nanoparticles (CPNs) are presented. CPNs are intrinsic fluorescent materials used for various biological applications. While CPNs cause no toxicity, decreased CPN uptake is observed from cancer cells pretreated with genistein, which is an inhibitor of caveolae-mediated endocytosis (CvME). CvME is further confirmed by high co-localization with caveolin-1 proteins found in the caveolae and caveosomes. Excellent photophysical properties, non-toxicity, and non-destructive delivery pathways support that CPNs are promising multifunctional carriers minimizing degradation of contents during delivery.
authors: Department of Chemistry, Florida International University, Miami, FL 33199, USA.
authors: Junghan
authors: J
authors: Lee
authors: Megan
authors: M
authors: Twomey
authors: Christian
authors: C
authors: Machado
authors: Giselle
authors: G
authors: Gomez
authors: Mona
authors: M
authors: Doshi
authors: Andre J
authors: AJ
authors: Gesquiere
authors: Joong Ho
authors: JH
authors: Moon
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D021941:Caveolae / Q000737:chemistry; D051242:Caveolin 1 / Q000737:chemistry / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000187:drug effects; D002478:Cells, Cultured; D016503:Drug Delivery Systems; D004705:Endocytosis; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D011108:Polymers / Q000008:administration & dosage / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D051242:Caveolin 1; D005456:Fluorescent Dyes; D011108:Polymers
keywords: conjugated polyelectrolytes; conjugated polymer nanoparticles; conjugated polymers; endocytosis mechanism; small interfering RNA delivery
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