title: Probe design rules and effective enzymes for endonuclease-based detection of nucleic acids.
abstract: Junction probe (JP) platform is an isothermal endonuclease-based detection assay for both RNA and DNA. Herein, we screen 31 REAse and identify effective restriction endonucleases that can be used for JP detection. Secondly, we investigate how different probe architectures affect JP cleavage rates and conclude that although molecular beacon (MB) JP probes give less background noise than linear JP probes, the cleavage of MB JP probes are slower than linear JP probes.
authors: University of Maryland, College Park, MD 20742, USA.
authors: Lei
authors: L
authors: Yan
authors: Shizuka
authors: S
authors: Nakayama
authors: Herman O
authors: HO
authors: Sintim
mesh_terms: D004247:DNA / Q000032:analysis* / Q000737:chemistry; D004262:DNA Restriction Enzymes / Q000737:chemistry*; D015345:Oligonucleotide Probes / Q000737:chemistry*; D012313:RNA / Q000032:analysis* / Q000737:chemistry
chemical_list: D015345:Oligonucleotide Probes; D012313:RNA; D004247:DNA; D004262:DNA Restriction Enzymes
keywords: Isothermal detection; Junction probe; Molecular beacon; Probe architecture; Restriction endonuclease