title: [The reduced pulmonary function is related to the decreased expression of Treg and Foxp3 in adjuvant-induced arthritis rats].
abstract: OBJECTIVE
  To observe changes of pulmonary function, regulatory T cells (Treg), forkhead transcription factor protein 3 (Foxp3) in adjuvant-induced arthritis (AA) rats, and explore the roles of Treg and Foxp3 expression in immunological mechanisms of AA.
  
  
  METHODS
  Thirty rats were randomly divided into normal control (NC) group and model control (MC) group, with 15 in each. The rats of MC group were induced by intracutaneous injection of 0.1 mL complete Freund's adjuvant in the right posterior paw. After 30 d, we observed the changes of the toe swelling degree, arthritis index. Pulmonary function was detected by animal spirometry, Treg in the peripheral blood by flow cytometry, and Foxp3 mRNA and protein by RT-PCR, immunohistochemistry, Western blotting, respectively. RESULTS Compared with the NC group, the toe swelling degree and arthritis index significantly increased, and the parameters of pulmonary function, CD4(+);CD25(+); Treg, CD4(+);CD25(+);FoxP3(+); Treg, Foxp3 mRNA and protein levels decreased in the MC group (P<0.01 or P<0.05).
  
  
  CONCLUSIONS
  AA rats suffer from reduced pulmonary function and immune dysfunction, which may be related to the decreased expressions of Treg and Foxp3.
authors: Department of Rheumatology and Immunology, Anhui College of Traditional Chinese Medicine, Hefei, China. yxwanlei@163.com
authors: Lei
authors: L
authors: Wan
authors: Jian
authors: J
authors: Liu
authors: Chuanbing
authors: C
authors: Huang
authors: Yuan
authors: Y
authors: Wang
authors: Lei
authors: L
authors: Liu
authors: Yuanyuan
authors: Y
authors: Cheng
authors: Yunxia
authors: Y
authors: Feng
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000276:immunology* / Q000378:metabolism / Q000503:physiopathology*; D015536:Down-Regulation; D051858:Forkhead Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D008168:Lung / Q000503:physiopathology*; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D050378:T-Lymphocytes, Regulatory / Q000473:pathology*; D014034:Toes / Q000473:pathology
chemical_list: D051858:Forkhead Transcription Factors; C500522:Foxp3 protein, rat; D012333:RNA, Messenger