title: Response to comment on "Evidence of abundant purifying selection in humans for recently acquired regulatory functions".
abstract: Green and Ewing propose corrections to our methodology, which we incorporate and extend here. The improved methodology supports our initial conclusion of extensive lineage-specific constraint concentrated in ENCODE elements. We clarify that our estimate is dependent on the constrained and neutral references used, which can further increase the number of nucleotides involved, because a particularly stringent definition was initially used.
authors: Computer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology (MIT), Cambridge, MA 02139, USA.
authors: Lucas D
authors: LD
authors: Ward
authors: Manolis
authors: M
authors: Kellis
mesh_terms: D005786:Gene Expression Regulation; D014644:Genetic Variation; D015894:Genome, Human / Q000235:genetics*; D006801:Humans; D012045:Regulatory Sequences, Nucleic Acid / Q000235:genetics*; D012641:Selection, Genetic
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