title: The adherens junction: a mosaic of cadherin and nectin clusters bundled by actin filaments.
abstract: Cadherin and nectin are distinct transmembrane proteins of adherens junctions. Their ectodomains mediate adhesion, whereas their cytosolic regions couple the adhesive contact to the cytoskeleton. Both these proteins are essential for adherens junction formation and maintenance. However, some basic aspects of these proteins, such as their organization in adherence junctions, have remained open. Therefore, using super-resolution microscopy and live imaging, we focused on the subjunctional distribution of these proteins. We showed that cadherin and nectin in the junctions of A431 cells and human keratinocytes are located in separate clusters. The size of each cluster is independent of that of the adjacent clusters and can significantly fluctuate over time. Several nectin and cadherin clusters that constitute an individual adherens junction are united by the same actin-filament bundle. Surprisingly, interactions between each cluster and F-actin are not uniform, as neither vinculin nor LIM-domain actin-binding proteins match the boundaries of cadherin or nectin clusters. Thus, the adherens junction is not a uniform structure but a mosaic of different adhesive units with very diverse modes of interaction with the cytoskeleton. We propose that such a mosaic architecture of adherence junctions is important for the fast regulation of their dynamics.
authors: Department of Dermatology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA.
authors: Indrajyoti
authors: I
authors: Indra
authors: Department of Dermatology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA.
authors: Soonjin
authors: S
authors: Hong
authors: Department of Dermatology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA. Electronic address: s-troyanovsky@northwestern.edu.
authors: Regina
authors: R
authors: Troyanovsky
authors: Department of Dermatology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA.
authors: Bernadett
authors: B
authors: Kormos
authors: Department of Dermatology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA.
authors: Sergey
authors: S
authors: Troyanovsky
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism*; D000199:Actins / Q000378:metabolism; D022005:Adherens Junctions / Q000378:metabolism*; D000818:Animals; D015820:Cadherins / Q000235:genetics / Q000378:metabolism*; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism; D000075983:Nectins; D034741:RNA, Small Interfering / Q000235:genetics; D059008:Time-Lapse Imaging; D014162:Transfection
chemical_list: D000199:Actins; D015820:Cadherins; D015815:Cell Adhesion Molecules; D008840:Microfilament Proteins; D000075983:Nectins; D034741:RNA, Small Interfering; C108835:afadin; D049452:Green Fluorescent Proteins
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