title: Modification-free photocontrol of β-lactam conversion with spatiotemporal resolution.
abstract: β-Lactams can be converted into β-amino acids by β-lactamase, a bacterial enzyme, leading to significant change in the biological function of the substrate molecules. Here we describe a method for photocontrol of β-lactam conversion without gene nor enzyme modification. This is achieved by the addition of a cationic photosensitive surfactant, AzoTAB, to a gene expression medium containing DNA coding for β-lactamase, the enzyme capable of the desired conversion. In the absence of UV (365 nm) or after illumination by blue light (480 nm) for 4 min, conversion of β-lactam is strongly reduced while the application of UV for 4 min results in a strong enhancement of substrate conversion. Several cycles of activation/inhibition are obtained upon successive UV/blue light illuminations. When both reconstituted photoresponsive gene expression medium and β-lactamase substrate are encapsulated in independent microfluidic chambers, selective UV illumination results in spatially resolved activation of substrate conversion.
authors: Anna
authors: A
authors: Venancio-Marques
authors: Yan-Jun
authors: YJ
authors: Liu
authors: Antoine
authors: A
authors: Diguet
authors: Thomas
authors: T
authors: di Maio
authors: Arnaud
authors: A
authors: Gautier
authors: Damien
authors: D
authors: Baigl
mesh_terms: D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods; D008958:Models, Molecular; D055668:Photochemical Processes; D017319:Photosensitizing Agents / Q000494:pharmacology; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology; D014466:Ultraviolet Rays; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism*; D047090:beta-Lactams / Q000378:metabolism*
chemical_list: D017319:Photosensitizing Agents; D000644:Quaternary Ammonium Compounds; D013501:Surface-Active Agents; D047090:beta-Lactams; C006664:4-azobenzenetrimethylammonium; D001618:beta-Lactamases