title: Expression of insulin-like growth factor 1 receptor in rat retina following optic nerve injury.
abstract: OBJECTIVE
  To investigate the apoptosis in retinal ganglion cells (RGCs) and insulin-like growth factor 1 receptor (IGF-1R) in the retina following optic nerve crush.
  
  
  METHODS
  Healthy Wistar rats (N = 70) were divided into two groups: a normal control group and an optic nerve injury group. Immunohistochemistry and flow cytometry were performed to detect the expression of IGF-1R and to measure the apoptosis of RGCs, respectively.
  
  
  RESULTS
  Immunohistochemistry revealed that at 1 hr after optic nerve injury, IGF-1R immunoreactivity began to increase and reached a maximal level at 24 hr (p < 0.05), where it remained elevated up to 14 days after injury. RGC apoptosis in the normal control group was 0.53%, while the apoptosis rate in the optic nerve injury group increased over time. The apoptosis rate in the optic nerve injury group was 1.4% at 1 hr, 4.4% at 6 hr, 5.2% at 12 hr and reached a maximal level (8.5%) at 24 hr. Subsequently, the rate declined to 1.9% 7 days after injury and 0.9% 2 weeks after injury.
  
  
  CONCLUSIONS
  The IGF-1R immunereactivity in the retina increased after optic nerve injury. IGF-1R may regulate the apoptosis and regeneration of RGCs at different stages after optic nerve injury.
authors: Department of Ophthalmology, The Second Affiliated Hospital of Soochow University, Suzhou, China. zhoucongying@163.com
authors: Shu-yang
authors: SY
authors: Bu
authors: Ge-hua
authors: GH
authors: Yu
authors: Guo-xu
authors: GX
authors: Xu
mesh_terms: D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D005434:Flow Cytometry; D007150:Immunohistochemistry; D008297:Male; D009409:Nerve Crush; D020221:Optic Nerve Injuries / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D017526:Receptor, IGF Type 1 / Q000378:metabolism*; D012160:Retina / Q000378:metabolism*; D012165:Retinal Ganglion Cells / Q000378:metabolism / Q000473:pathology
chemical_list: D017526:Receptor, IGF Type 1
keywords: apoptosis; insulin-like growth factor 1; insulin-like growth factor 1 receptor; optic nerve injury; retinal ganglion cell