title: Pif1 family helicases suppress genome instability at G-quadruplex motifs.
abstract: The Saccharomyces cerevisiae Pif1 helicase is the prototypical member of the Pif1 DNA helicase family, which is conserved from bacteria to humans. Here we show that exceptionally potent G-quadruplex unwinding is conserved among Pif1 helicases. Moreover, Pif1 helicases from organisms separated by more than 3 billion years of evolution suppressed DNA damage at G-quadruplex motifs in yeast. The G-quadruplex-induced damage generated in the absence of Pif1 helicases led to new genetic and epigenetic changes. Furthermore, when expressed in yeast, human PIF1 suppressed both G-quadruplex-associated DNA damage and telomere lengthening.
authors: Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA.
authors: Katrin
authors: K
authors: Paeschke
authors: Matthew L
authors: ML
authors: Bochman
authors: P Daniela
authors: PD
authors: Garcia
authors: Petr
authors: P
authors: Cejka
authors: Katherine L
authors: KL
authors: Friedman
authors: Stephen C
authors: SC
authors: Kowalczykowski
authors: Virginia A
authors: VA
authors: Zakian
mesh_terms: D001483:Base Sequence; D017124:Conserved Sequence; D004249:DNA Damage / Q000235:genetics; D004265:DNA Helicases / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D044127:Epigenesis, Genetic; D019143:Evolution, Molecular; D054856:G-Quadruplexes; D020868:Gene Silencing; D005816:Genetic Complementation Test; D042822:Genomic Instability; D006801:Humans; D008969:Molecular Sequence Data; D059645:Mutation Rate; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D059505:Telomere Homeostasis / Q000235:genetics
chemical_list: D029701:Saccharomyces cerevisiae Proteins; C085463:PIF1 protein, S cerevisiae; D004265:DNA Helicases; C508765:PIF1 protein, human
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