title: Optoelectronic tweezers integrated with lensfree holographic microscopy for wide-field interactive cell and particle manipulation on a chip.
abstract: We demonstrate an optoelectronic tweezer (OET) coupled to a lensfree holographic microscope for real-time interactive manipulation of cells and micro-particles over a large field-of-view (FOV). This integrated platform can record the holographic images of cells and particles over the entire active area of a CCD sensor array, perform digital image reconstruction to identify target cells, dynamically track the positions of cells and particles, and project light beams to trigger light-induced dielectrophoretic forces to pattern and sort cells on a chip. OET technology has been previously shown to be capable of performing parallel single cell manipulation over a large area. However, its throughput has been bottlenecked by the number of cells that can be imaged within the limited FOV of a conventional microscope objective lens. Integrating lensfree holographic imaging with OET solves this fundamental FOV barrier, while also creating a compact on-chip cell/particle manipulation platform. Using this unique platform, we have successfully demonstrated real-time interactive manipulation of thousands of single cells and micro-particles over an ultra-large area of e.g., 240 mm(2) (i.e. 17.96 mm × 13.52 mm).
authors: Department of Mechanical and Aerospace Engineering, University of California, Los Angeles, CA 90095, USA.
authors: Kuo-Wei
authors: KW
authors: Huang
authors: Ting-Wei
authors: TW
authors: Su
authors: Aydogan
authors: A
authors: Ozcan
authors: Pei-Yu
authors: PY
authors: Chiou
mesh_terms: D000465:Algorithms; D006367:HeLa Cells; D006696:Holography; D006801:Humans; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D052898:Optical Tweezers; D017971:Tin Compounds / Q000737:chemistry
chemical_list: D017971:Tin Compounds; C109984:indium tin oxide
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