title: Cryptococcus gattii in urban trees from cities in North-eastern Argentina.
abstract: In the city of Buenos Aires, Argentina, Cryptococcus gattii genotype AFLP4/VGI was found to be associated with decaying wood in hollows of different tree species. The aim of this study was to investigate the presence of C. gattii in the environment of riverside cities of the river Paraná, and to describe its serotypes and molecular types. Five hundred samples were collected in 50 parks by swabbing tree hollows. The samples were inoculated on caffeic acid agar supplemented with chloramphenicol, and incubated at 28 °C for 1 week with a daily observation. The isolates were identified by conventional methods. The serotype was determined by slide agglutination with specific antisera. Molecular typing was carried out by PCR-RFLP of the URA5 gene. Four isolates of C. gattii were recovered: Cryptococcus gattii serotype B, genotype AFLP4/VGI, isolated from Eucalyptus sp. in the city of Rosario and from Grevillea robusta in the city of La Paz; and C. gattii serotype C, genotype AFLP5/VGIII, isolated from two different Tipuana tipu trees in the city of Resistencia. Here, we report for the first time the isolation of C. gattii serotype C, genotype AFLP5/VGIII, from environmental samples in Argentina.
authors: Mycology Department, INEI 'Dr. Carlos G. Malbrán'- ANLIS. Ciudad Autónoma de Buenos Aires, Argentina.
authors: Mariana
authors: M
authors: Mazza
authors: Nicolás
authors: N
authors: Refojo
authors: María Eugenia
authors: ME
authors: Bosco-Borgeat
authors: Constanza Giselle
authors: CG
authors: Taverna
authors: Alicia Cristina
authors: AC
authors: Trovero
authors: Ariel
authors: A
authors: Rogé
authors: Graciela
authors: G
authors: Davel
mesh_terms: D000372:Agglutination Tests; D001118:Argentina; D056285:Cryptococcus gattii / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D007887:Fabaceae / Q000382:microbiology; D005838:Genotype; D008828:Microbiological Techniques; D058889:Molecular Typing; D016533:Mycological Typing Techniques; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D039105:Proteaceae / Q000382:microbiology; D012703:Serotyping; D014197:Trees / Q000382:microbiology*
keywords: Cryptococcus gattii; Environmental isolation; Epidemiology; Genotyping; Serotyping