title: Paracoccus zhejiangensis sp. nov., isolated from activated sludge in wastewater-treatment system.
abstract: A bacterial strain, designated J6(T), was isolated from activated sludge, collected from a chemical wastewater treatment system in Zhejiang Province of China. The cells stained Gram-negative, were aerobic, pale-yellow, and non-motile short rods. Phylogenetic analysis of the 16S rRNA gene sequence indicated that the closest relative of this organism was Paracoccus aminophilus KACC 12262(T) = JCM 7686(T) (97.4 % sequence similarity). Strain J6(T) grew at 10-37 °C (optimum 30 °C), at pH 6.0-8.0 (optimum pH 7.0) and with 0-5 % NaCl (optimum 3 %, w/v). The predominant cellular fatty acid found was summed feature 8(C18:1 ω7c and/or C18:1 ω6c; 82.8 %). The major respiratory quinone-detected was Q-10 and the DNA G+C content was 61.9 mol %. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine and several unknown polar lipids. Strain J6(T) showed low DNA-DNA relatedness values with P. aminophilus KACC 12262(T) (28 ± 3 %). The phylogenetic analysis, DNA-DNA hybridization, whole-cell fatty acid composition as well as biochemical characteristics allowed clear differentiation of the isolate from the other type strains of already described Paracoccus species. It is evident from the genotypic, phenotypic and chemotaxonomic analyses that strain J6(T) should be classified as a novel species of the genus Paracoccus, for which the name P. zhejiangensis sp. nov. is proposed. The type strain is J6(T) (KACC 16703(T) = CCTCC AB 2012031(T)).
authors: Key Lab of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, Jiangsu, People's Republic of China.
authors: Zhi-Guo
authors: ZG
authors: Wu
authors: Dao-Feng
authors: DF
authors: Zhang
authors: Ya-Long
authors: YL
authors: Liu
authors: Fang
authors: F
authors: Wang
authors: Xin
authors: X
authors: Jiang
authors: Chao
authors: C
authors: Li
authors: Shun-Peng
authors: SP
authors: Li
authors: Qing
authors: Q
authors: Hong
authors: Wen-Jun
authors: WJ
authors: Li
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001482:Base Composition; D001483:Base Sequence; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000032:analysis; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D010230:Paracoccus / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D010641:Phenotype; D010802:Phylogeny; D011809:Quinones / Q000032:analysis; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D012722:Sewage / Q000382:microbiology*; D013045:Species Specificity; D013696:Temperature; D062065:Wastewater; D014871:Water Microbiology
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D005227:Fatty Acids; D008055:Lipids; D011809:Quinones; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D062065:Wastewater