title: The increase in cholesterol levels at early stages after dengue virus infection correlates with an augment in LDL particle uptake and HMG-CoA reductase activity.
abstract: Several cellular molecules and components, specifically, cholesterol and lipid rafts have been described as necessary elements for dengue virus entry and signaling in several human cells. Thus, changes in lipid rafts formation and cholesterol levels were evaluated. Here we report that the amount of total cholesterol and lipid rafts formation increase early after infection of Huh-7 cells. This augment correlates with an increase in the amount of low density lipoprotein receptor (LDLr) on the surface of infected cells and also with a lower phosphorylation level of the 3-hydroxy-3-methyl-glutaryl-CoA reductase (HMGCR). None of the changes were observed in Huh 7 cells infected with VSV used as a control. These results suggest that dengue virus infection increases intracellular cholesterol levels at early times post infection by triggering the modulation of LDL particles uptake and the increase in the enzymatic activity of HMG-CoA reductase.
authors: Departamento de Infectómica y Patogénesis Molecular, CINVESTAV-IPN, México, D.F., Mexico.
authors: Rubén
authors: R
authors: Soto-Acosta
authors: Clemente
authors: C
authors: Mosso
authors: Margot
authors: M
authors: Cervantes-Salazar
authors: Henry
authors: H
authors: Puerta-Guardo
authors: Fernando
authors: F
authors: Medina
authors: Liliana
authors: L
authors: Favari
authors: Juan E
authors: JE
authors: Ludert
authors: Rosa María
authors: RM
authors: del Angel
mesh_terms: D002460:Cell Line; D008078:Cholesterol, LDL / Q000378:metabolism*; D003716:Dengue Virus / Q000502:physiology*; D022781:Hepatocytes / Q000737:chemistry / Q000821:virology; D054884:Host-Pathogen Interactions; D006801:Humans; D006903:Hydroxymethylglutaryl CoA Reductases / Q000378:metabolism*; D021962:Membrane Microdomains / Q000378:metabolism*; D014779:Virus Replication
chemical_list: D008078:Cholesterol, LDL; D006903:Hydroxymethylglutaryl CoA Reductases