title: The yeast cell cortical protein Num1 integrates mitochondrial dynamics into cellular architecture.
abstract: During the cell cycle each organelle has to be faithfully partitioned to the daughter cells. However, the mechanisms controlling organellar inheritance remain poorly understood. We studied the contribution of the cell cortex protein, Num1, to mitochondrial partitioning in yeast. Live-cell microscopy revealed that Num1 is required for attachment of mitochondria to the cell cortex and retention in mother cells. Electron tomography of anchoring sites revealed plasma membrane invaginations directly contacting the mitochondrial outer membrane. Expression of chimeric plasma membrane tethers rescued mitochondrial fission defects in Δnum1 and Δmdm36 mutants. These findings provide new insights into the coupling of mitochondrial dynamics, immobilization, and retention during inheritance.
authors: Zellbiologie, Universität Bayreuth, 95440 Bayreuth, Germany.
authors: Till
authors: T
authors: Klecker
authors: Dirk
authors: D
authors: Scholz
authors: Johannes
authors: J
authors: Förtsch
authors: Benedikt
authors: B
authors: Westermann
mesh_terms: D002453:Cell Cycle / Q000235:genetics*; D003593:Cytoplasm / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D003598:Cytoskeletal Proteins / Q000172:deficiency / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008928:Mitochondria / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D063154:Mitochondrial Dynamics / Q000235:genetics*; D051336:Mitochondrial Membranes / Q000378:metabolism / Q000648:ultrastructure; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D059008:Time-Lapse Imaging
chemical_list: D003598:Cytoskeletal Proteins; C071961:NUM1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D049452:Green Fluorescent Proteins
keywords: Cell architecture; Mitochondrial fission; Organelle partitioning