title: Fabrication of reduced graphene oxide and sliver nanoparticle hybrids for Raman detection of absorbed folic acid: a potential cancer diagnostic probe.
abstract: Reduced graphene oxide (RGO) and silver nanoparticle (AgNP) hybrids (RGO-AgNP) were prepared by a facile one-pot method using Poly (N-vinyl-2-pyrrolidone) as reductant and stabilizer. Folic acid (FA) molecules were attached to the RGO-AgNP by physisorption for targeting specific cancer cells with folate receptors (FRs) and using as Raman reporter molecules. The internalization of the FA loaded RGO-AgNP (RGO-AgNP-FA) inside the FRs-positive cancer cell was confirmed by confocal laser scanning and transmission electron microscopy. The Raman signals of the FA in live cancer cells were detected by confocal Raman spectroscope at 514 nm excitation, indicating that the RGO-AgNP-FA material has great potential as a Raman probe for cancer diagnosis in vitro.
authors: Department of Chemistry, Jinan University, Guangzhou 510632, P R China.
authors: Chaofan
authors: C
authors: Hu
authors: Yingliang
authors: Y
authors: Liu
authors: Jinlan
authors: J
authors: Qin
authors: Guangting
authors: G
authors: Nie
authors: Bingfu
authors: B
authors: Lei
authors: Yong
authors: Y
authors: Xiao
authors: Mingtao
authors: M
authors: Zheng
authors: Jianhua
authors: J
authors: Rong
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods; D020099:Coated Materials, Biocompatible / Q000493:pharmacokinetics / Q000633:toxicity; D005260:Female; D005492:Folic Acid / Q000493:pharmacokinetics*; D006108:Graphite / Q000138:chemical synthesis* / Q000633:toxicity; D006367:HeLa Cells; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D046529:Microscopy, Electron, Transmission; D010087:Oxides / Q000138:chemical synthesis / Q000633:toxicity; D012834:Silver / Q000737:chemistry* / Q000633:toxicity; D013059:Spectrum Analysis, Raman / Q000379:methods*; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D014803:Vitamin B Complex / Q000493:pharmacokinetics
chemical_list: D020099:Coated Materials, Biocompatible; D010087:Oxides; D014803:Vitamin B Complex; D012834:Silver; D006108:Graphite; D005492:Folic Acid