title: Low erythrocyte catalase enzyme activity is correlated with high serum total homocysteine levels in Tunisian patients with acute myocardial infarction.
abstract: BACKGROUND
  An imbalance between pro-oxidants and antioxidant systems has been suggested to be implicated in the physiopathology of acute myocardial infarction (AMI). We aimed to evaluate the antioxidant capacity in Tunisian patients and to assess the possible relationship between erythrocyte catalase enzyme activity and hyperhomocysteinaemia.
  
  
  METHODS
  108 patients with AMI and 81 healthy subjects were enrolled in this study. Catalase erythrocyte enzyme activity was determined spectrophotometrically whereas "total antioxidant status" (TAS) concentration was measured by a commercially available method. Serum total homocysteine (tHcy) level was determined by a fluorescence polarization immunoassay (FPIA). Lipid peroxidation was measured with a fluorimetric method as "thiobarbituric acid reactive substances" (TBARS).
  
  
  RESULTS
  Compared with healthy subjects, patients with AMI had significantly lower catalase activity (P<0.001), TAS concentrations (P<0.001), and significantly higher serum tHcy (P<0.001) and TBARS levels (P<0.001). Erythrocyte catalase enzyme activity was negatively correlated with serum tHcy and TBARS while serum tHcy and TBARS were in positive correlation. Furthermore, the unbalance between pro-oxidants and antioxidants seems to be more aggravated in patients with Q wave AMI compared to patients with non-Q wave AMI.
  
  
  CONCLUSIONS
  Our results suggest the involvement of hyperhomocysteinaemia in the drop of erythrocyte catalase activity related to myocardial ischemia reperfusion. Hyperhomocysteinaemia may increase the myocardial wall dysfunction under ischemia reperfusion by excessive production of reactive oxygen species which is made evident by increased lipid peroxidation.
  The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1623509866881834.
authors: Biochemistry Laboratory CHU Farhat HACHED, Street Doctor Moreau, 4000 Sousse, Tunisia. Yosri.sousse@gmail.com
authors: Yosri
authors: Y
authors: Noichri
authors: Abdelkader
authors: A
authors: Chalghoum
authors: Latifa
authors: L
authors: Chkioua
authors: Bruno
authors: B
authors: Baudin
authors: Samia
authors: S
authors: Ernez
authors: Salima
authors: S
authors: Ferchichi
authors: Abdelhédi
authors: A
authors: Miled
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000975:Antioxidants / Q000378:metabolism; D002374:Catalase / Q000097:blood*; D004912:Erythrocytes / Q000201:enzymology*; D005260:Female; D006710:Homocysteine / Q000097:blood*; D006801:Humans; D015227:Lipid Peroxidation / Q000502:physiology; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000201:enzymology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014416:Tunisia
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D006710:Homocysteine; D002374:Catalase
references: Eur J Biochem. 1995 Aug 15;232(1):188-91
references: 7556149
references: Diagn Pathol. 2011 Jan 19;6:8
references: 21244717
references: Clin Chim Acta. 2004 Oct;348(1-2):131-7
references: 15369746
references: Ann Biol Clin (Paris). 2012 Oct 1;70(5):599-604
references: 23047907
references: Life Sci. 2006 Sep 5;79(15):1466-72
references: 16737718
references: Vision Res. 2010 Mar 31;50(7):652-64
references: 19703486
references: Free Radic Biol Med. 1999 Jul;27(1-2):28-33
references: 10443916
references: Toxicol In Vitro. 2007 Dec;21(8):1402-8
references: 17590309
references: Mol Aspects Med. 2008 Feb-Apr;29(1-2):1-8
references: 18037483
references: Diagn Pathol. 2008 Mar 19;3:12
references: 18353184
references: Biochem Med. 1976 Apr;15(2):212-6
references: 962904
references: Cardiovasc Res. 2004 Feb 15;61(3):461-70
references: 14962477
references: Int J Cardiol. 2007 Apr 25;117(2):260-9
references: 16860887
references: Rev Epidemiol Sante Publique. 2004 Dec;52(6):558-64
references: 15741917
references: J Cardiovasc Nurs. 2006 Mar-Apr;21(2):118-22
references: 16601528
references: Am J Med. 2000 Sep;109(4):315-23
references: 10996583
references: Clin Chim Acta. 1991 Feb 15;196(2-3):143-51
references: 2029780
references: Int J Biomed Sci. 2008 Jun;4(2):89-96
references: 23675073
references: Dis Mon. 2006 May;52(5):183-98
references: 16828360
references: Curr Opin Pharmacol. 2004 Apr;4(2):159-65
references: 15063360
references: Clin Sci (Lond). 1993 Apr;84(4):407-12
references: 8482045
references: Ann Clin Biochem. 2008 Mar;45(Pt 2):193-8
references: 18325185
references: Biochim Biophys Acta. 2004 Jan 20;1688(1):33-42
references: 14732479
references: Free Radic Biol Med. 2003 Oct 15;35(8):882-8
references: 14556852
references: Circulation. 2001 Sep 4;104(10):1165-70
references: 11535574
references: Toxicol Appl Pharmacol. 2008 Oct 1;232(1):99-108
references: 18634817