title: The developmental regulation of glutamate receptor-mediated calcium signaling in primary cultured rat hippocampal neurons.
abstract: We have studied the developmental changes of glutamate-induced calcium (Ca²⁺) response in primary cultured hippocampal neurons at three different stages of cultures, 3, 7-8, and 14-16 days in vitro (DIV), using fura-2 single-cell digital micro-fluorimetry. We found that glutamate-induced Ca²⁺ signaling was altered during development, and that two different ionotropic glutamate receptors, α-amino-3-hydroxy-5-methyl-4-isoxazole propionate receptors (AMPARs) and N-methyl-D-aspartate receptors (NMDARs), were differently involved in the modulation of calcium response at different stages of neuronal culture. In the stages of culture at 3 and 8 DIV, glutamate-induced Ca²⁺ influx was mostly because of AMPAR activation and subsequent opening of voltage-dependent calcium channels, as Ca²⁺ response can be largely reduced by 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) and by nifedipine. In the advanced culture (14-17 DIV), glutamate-induced Ca²⁺ response was less sensitive to 6-cyano-7-nitroquinoxaline-2,3-dione and nifedipine. Furthermore, AMPA-induced Ca²⁺ response increased in a time-dependent manner during the cultures of 3-8 DIV and then reduced in the advanced culture of 14-17 DIV. NMDA-induced Ca²⁺ influx increased in a time-dependent manner, with a marked increase in the advanced culture (14-17 DIV). These results suggest that glutamate-induced Ca²⁺ signaling switched from AMPA-voltage-dependent calcium channel to NMDA-calcium signaling during development.
authors: Department of Physiology and Neurobiology, Xinxiang Medical University, Xinxiang, People's Republic of China.
authors: Zhi Y
authors: ZY
authors: Guo
authors: Cheng Z
authors: CZ
authors: Li
authors: Xiao J
authors: XJ
authors: Li
authors: Ya L
authors: YL
authors: Wang
authors: Mark P
authors: MP
authors: Mattson
authors: Cheng B
authors: CB
authors: Lu
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D018683:Excitatory Amino Acid Agents / Q000494:pharmacology; D018698:Glutamic Acid / Q000494:pharmacology; D006624:Hippocampus / Q000166:cytology*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017470:Receptors, Glutamate / Q000378:metabolism*
chemical_list: D018683:Excitatory Amino Acid Agents; D017470:Receptors, Glutamate; D018698:Glutamic Acid; D002118:Calcium