title: Bismuth oxide aqueous colloidal nanoparticles inhibit Candida albicans growth and biofilm formation.
abstract: Multiresistance among microorganisms to common antimicrobials has become one of the most significant concerns in modern medicine. Nanomaterials are a new alternative to successfully treat the multiresistant microorganisms. Nanostructured materials are used in many fields, including biological sciences and medicine. Recently, it was demonstrated that the bactericidal activity of zero-valent bismuth colloidal nanoparticles inhibited the growth of Streptococcus mutans; however the antimycotic potential of bismuth nanostructured derivatives has not yet been studied. The main objective of this investigation was to analyze the fungicidal activity of bismuth oxide nanoparticles against Candida albicans, and their antibiofilm capabilities. Our results showed that aqueous colloidal bismuth oxide nanoparticles displayed antimicrobial activity against C. albicans growth (reducing colony size by 85%) and a complete inhibition of biofilm formation. These results are better than those obtained with chlorhexidine, nystatin, and terbinafine, the most effective oral antiseptic and commercial antifungal agents. In this work, we also compared the antimycotic activities of bulk bismuth oxide and bismuth nitrate, the precursor metallic salt. These results suggest that bismuth oxide colloidal nanoparticles could be a very interesting candidate as a fungicidal agent to be incorporated into an oral antiseptic. Additionally, we determined the minimum inhibitory concentration for the synthesized aqueous colloidal Bi2O3 nanoparticles.
authors: Facultad de Ciencias Biológicas, Instituto de Biotecnologia, Universidad Autonoma de Nuevo Leon, UANL, Monterrey, Mexico.
authors: Rene
authors: R
authors: Hernandez-Delgadillo
authors: Donaji
authors: D
authors: Velasco-Arias
authors: Juan Jose
authors: JJ
authors: Martinez-Sanmiguel
authors: David
authors: D
authors: Diaz
authors: Inti
authors: I
authors: Zumeta-Dube
authors: Katiushka
authors: K
authors: Arevalo-Niño
authors: Claudio
authors: C
authors: Cabral-Romero
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D001729:Bismuth / Q000737:chemistry / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects* / Q000502:physiology; D048430:Cell Shape / Q000187:drug effects; D002710:Chlorhexidine / Q000494:pharmacology; D002522:Chlorocebus aethiops; D053768:Metal Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D008856:Microscopy, Fluorescence; D009281:Naphthalenes / Q000494:pharmacology; D000077291:Terbinafine; D014709:Vero Cells
chemical_list: D000935:Antifungal Agents; D009281:Naphthalenes; C033301:bismuth oxide; D000077291:Terbinafine; D002710:Chlorhexidine; D001729:Bismuth
keywords: antifungal; antimycotic agent; cytotoxicity
references: Enferm Infecc Microbiol Clin. 2011 Dec;29 Suppl 5:38-44
references: 22305668
references: Nat Biotechnol. 2003 Oct;21(10):1166-70
references: 14520401
references: J Ind Microbiol. 1995 Sep;15(3):137-40
references: 8519468
references: PLoS One. 2006 Dec 20;1:e11
references: 17183637
references: J Immunol Methods. 1983 Dec 16;65(1-2):55-63
references: 6606682
references: Mol Microbiol. 2012 Nov;86(3):539-56
references: 22924823
references: J Microbiol Biotechnol. 2008 Aug;18(8):1482-4
references: 18756112
references: Cytometry. 1995 Nov 1;21(3):265-74
references: 8582249
references: Int J Antimicrob Agents. 2009 Jun;33(6):587-90
references: 19195845
references: J Colloid Interface Sci. 2004 Jul 1;275(1):177-82
references: 15158396
references: J Neurochem. 1997 Aug;69(2):581-93
references: 9231715
references: Rev Environ Contam Toxicol. 2012;215:39-121
references: 22057930
references: Int J Nanomedicine. 2010 Dec 06;5:1085-94
references: 21270959
references: J Med Microbiol. 2013 Jan;62(Pt 1):10-24
references: 23180477
references: Appl Environ Microbiol. 2005 Nov;71(11):7589-93
references: 16269810
references: Saudi J Biol Sci. 2012 Apr;19(2):147-50
references: 23961173
references: Prog Neurobiol. 2009 Feb;87(3):133-70
references: 18926873
references: Nanomedicine. 2007 Mar;3(1):95-101
references: 17379174
references: N Engl J Med. 1993 Jun 10;328(23):1653-8
references: 8487823
references: FEBS J. 2012 Apr;279(7):1327-38
references: 22324978
references: Obstet Gynecol. 2012 Dec;120(6):1407-14
references: 23168767
references: Biochemistry. 1987 Jul 14;26(14):4545-53
references: 3663606
references: Phys Chem Chem Phys. 2008 Dec 21;10(47):7092-7
references: 19039343
references: J Bacteriol. 2001 Sep;183(18):5385-94
references: 11514524
references: J Biomed Mater Res. 2000 Dec 15;52(4):662-8
references: 11033548
references: Nanotechnology. 2011 Mar 11;22(10):105101
references: 21289395
references: Can J Microbiol. 1974 Jun;20(6):883-9
references: 4151872
references: ISRN Dent. 2012;2012:469019
references: 23119173
references: Int J Nanomedicine. 2012;7:2109-13
references: 22619547
references: J Antimicrob Chemother. 2001 Jul;48 Suppl 1:5-16
references: 11420333
references: Nucleic Acids Res. 2001 Apr 15;29(8):E41-1
references: 11292855
references: IUBMB Life. 2007 Apr-May;59(4-5):255-62
references: 17505962
references: Int J Nanomedicine. 2011;6:1553-8
references: 21845045
references: Acta Med Philipp. 1956 Jul-1957 Jun;13(1-4):151-5
references: 13508064
references: Nanomedicine. 2008 Jun;4(2):167-71
references: 18329962
references: Inorg Chem. 2011 Sep 5;50(17):7910-2
references: 21797245