title: Role of quercetin in cadmium-induced oxidative stress, neuronal damage, and apoptosis in rats.
abstract: The present study was carried out to evaluate the neuroprotective effect of quercetin (QE) in protecting the cadmium (Cd)-induced neuronal injury in frontal cortex of rats. A total of 30 adult male Sprague-Dawley rats were randomly divided into three groups of 10 animals each: control, Cd treated and Cd treated with QE. The Cd-treated group was injected subcutaneously with cadmium chloride (CdCl2) dissolved in saline at a dose of 2 ml/kg/day for 30 days, resulting in a dosage of 1 mg/kg Cd. The rats in QE-treated groups were given QE (15 mg/kg body weight) once a day intraperitoneally starting 2 days prior to Cd injection, during the study period. Rats were sacrificed at the end of the study and the frontal cortex tissues were removed for biochemical and histopathological investigation. To date, there is no available information on the effect of QE on neuronal injury after Cd exposure. Rats intoxicated with Cd for 30 days, significantly increased tissue malondialdehyde (MDA) levels and significantly decreased enzymatic antioxidants superoxide dismutase, glutathione peroxidase and catalase in the frontal cortex tissue. Administration of QE with Cd significantly diminished the levels of MDA and significantly elevated the levels of enzymatic antioxidants in the frontal cortex tissue. The histopathological studies in the brain of rats also supported that QE markedly reduced the Cd-induced histopathological changes and well preserved the normal histological architecture of the frontal cortex tissue. The caspase-3 immunopositivity was increased in degenerating neurons of the Cd group. Treatment with QE markedly reduced the immunoreactivity of degenerating neurons. In conclusion, the results of the current study suggest that QE may be beneficial in combating the Cd-induced neurotoxicity in the brain of rats. We believe that further preclinical research into the utility of QE may indicate its usefulness as a potential treatment for neurodegeneration after Cd exposure in rats.
authors: Department of Psychiatry, Namik Kemal University, Tekirdag, Turkey.
authors: Cüneyt
authors: C
authors: Unsal
authors: Department of Histology and Embryology, Istanbul Medeniyet University, Istanbul, Turkey mkanter65@yahoo.com.
authors: Mehmet
authors: M
authors: Kanter
authors: Department of Histology and Embryology, Namik Kemal University, Tekirdag, Turkey.
authors: Cevat
authors: C
authors: Aktas
authors: Department of Histology and Embryology, Namik Kemal University, Tekirdag, Turkey.
authors: Mustafa
authors: M
authors: Erboga
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D019256:Cadmium Chloride / Q000008:administration & dosage; D002105:Cadmium Poisoning / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D053148:Caspase 3 / Q000737:chemistry / Q000378:metabolism; D005625:Frontal Lobe / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D007274:Injections, Intraperitoneal; D007279:Injections, Subcutaneous; D008297:Male; D008315:Malondialdehyde / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000819:agonists / Q000037:antagonists & inhibitors / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D018696:Neuroprotective Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D020258:Neurotoxicity Syndromes / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D018384:Oxidative Stress / Q000187:drug effects; D010088:Oxidoreductases / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000378:metabolism; D011794:Quercetin / Q000008:administration & dosage / Q000627:therapeutic use*; D011897:Random Allocation; D017207:Rats, Sprague-Dawley
chemical_list: D000975:Antioxidants; D009419:Nerve Tissue Proteins; D018696:Neuroprotective Agents; D008315:Malondialdehyde; D011794:Quercetin; D010088:Oxidoreductases; C505410:Casp3 protein, rat; D053148:Caspase 3; D019256:Cadmium Chloride
keywords: Cadmium; neuronal apoptosis; neuronal damage; oxidative stress; quercetin; rats