title: Impacts of single nucleotide polymorphisms and haplotypes in the bovine Dapper1 gene on body weight.
abstract: The Dapper1 protein plays important role in multiple developmental processes via negative modulation of the Wnt signaling pathway. We detected variations in Dapper1 in 1185 individuals from 5 Chinese cattle breeds and determined their effects on bovine body weight. Two silent mutations (g.8344C>T and g.8428C>T) in exon 6 along with two substitutions (g.10513A>G and g.10765C>G) in the 3'-untranslated region were detected with DNA pool sequencing and forced polymerase chain reaction-restriction fragment length polymorphism. Haplotype variability and the extent of linkage disequilibrium of the 4 single nucleotide polymorphisms (SNPs) were analyzed, and the results revealed 16 haplotypes and 7 combined haplotypes in the 5 cattle breeds. Statistical analyses indicated that genotypes CC and AA in the g.8344C>T and g.10513A>G loci were associated with heavier body weight at 6 months in the Nanyang cattle population (P < 0.05), and the combined haplotype had consistent significant effects on body weight with a single SNP. Cattle with haplotype combinations H1H5 (CCCTAACC) displayed the heaviest body weight at 6 months compared with that of other haplotypes (P < 0.05). Our results provide evidence that 4 SNPs and haplotypes in Dapper1 may be used for marker-assisted selection in beef cattle breeding programs.
authors: Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
authors: J
authors: J
authors: Wang
authors: C
authors: C
authors: Wang
authors: Y
authors: Y
authors: Gao
authors: X-Y
authors: XY
authors: Lan
authors: C-Z
authors: CZ
authors: Lei
authors: J-Q
authors: JQ
authors: Wang
authors: H
authors: H
authors: Chen
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D000483:Alleles; D000818:Animals; D001483:Base Sequence; D001835:Body Weight / Q000235:genetics*; D002417:Cattle; D005260:Female; D005787:Gene Frequency; D005828:Genetics, Population; D005838:Genotype; D006239:Haplotypes; D015810:Linkage Disequilibrium; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide; D019655:Quantitative Trait, Heritable
chemical_list: D048868:Adaptor Proteins, Signal Transducing