title: Integrin α1 has a long helix, extending from the transmembrane region to the cytoplasmic tail in detergent micelles.
abstract: Integrin proteins are very important adhesion receptors that mediate cell-cell and cell-extracellular matrix interactions. They play essential roles in cell signaling and the regulation of cellular shape, motility, and the cell cycle. Here, the transmembrane and cytoplasmic (TMC) domains of integrin α1 and β1 were over-expressed and purified in detergent micelles. The structure and backbone relaxations of α1-TMC in LDAO micelles were determined and analyzed using solution NMR. A long helix, extending from the transmembrane region to the cytoplasmic tail, was observed in α1-TMC. Structural comparisons of α1-TMC with reported αIIb-TMC domains indicated different conformations in the transmembrane regions and cytoplasmic tails. An NMR titration experiment indicated weak interactions between α1-TMC and β1-TMC through several α1-TMC residues located at its N-terminal juxta-transmembrane region and C-terminal extended helix region.
authors: Hefei National Laboratory for Physical Science at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, China.
authors: Chaohua
authors: C
authors: Lai
authors: Xiaoxi
authors: X
authors: Liu
authors: Changlin
authors: C
authors: Tian
authors: Fangming
authors: F
authors: Wu
mesh_terms: D003902:Detergents / Q000737:chemistry*; D004123:Dimethylamines / Q000737:chemistry; D006801:Humans; D039423:Integrin alpha1 / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D039641:Integrin beta Chains / Q000737:chemistry / Q000378:metabolism; D008823:Micelles; D008958:Models, Molecular; D019906:Nuclear Magnetic Resonance, Biomolecular; D011485:Protein Binding; D011487:Protein Conformation; D054730:Protein Interaction Domains and Motifs; D017433:Protein Structure, Secondary
chemical_list: D003902:Detergents; D004123:Dimethylamines; D039423:Integrin alpha1; D039641:Integrin beta Chains; D008823:Micelles; C014518:dodecyldimethylamine oxide
references: Am J Pathol. 2002 May;160(5):1779-85
references: 12000729
references: J Biol Chem. 2003 Feb 28;278(9):7270-7
references: 12496264
references: EMBO J. 2009 May 6;28(9):1351-61
references: 19279667
references: J Mol Biol. 2009 Oct 2;392(4):1087-101
references: 19527732
references: Nat Rev Mol Cell Biol. 2010 Apr;11(4):288-300
references: 20308986
references: J Am Chem Soc. 2012 Jun 6;134(22):9030-3
references: 22626249
references: Proc Natl Acad Sci U S A. 2002 Apr 30;99(9):5878-83
references: 11983888
references: J Biomol NMR. 1995 Nov;6(3):277-93
references: 8520220
references: Blood. 2008 Oct 15;112(8):3242-54
references: 18647959
references: J Biol Chem. 2002 Feb 15;277(7):4636-43
references: 11724770
references: Cell. 2002 Sep 20;110(6):673-87
references: 12297042
references: J Biol Chem. 2001 Dec 21;276(51):48206-12
references: 11572855
references: Biochemistry. 2012 Jan 17;51(2):631-42
references: 22242826
references: J Biol Chem. 2008 Jun 6;283(23):16162-8
references: 18417472
references: J Magn Reson. 2003 Jan;160(1):65-73
references: 12565051
references: Trends Biochem Sci. 2011 Apr;36(4):191-8
references: 21216149
references: Curr Rheumatol Rep. 1999 Oct;1(1):28-33
references: 11123011
references: Proc Natl Acad Sci U S A. 2000 Feb 29;97(5):2202-7
references: 10681423
references: Proc Natl Acad Sci U S A. 2010 Dec 28;107(52):22481-6
references: 21156831
references: Exp Cell Res. 2000 Apr 10;256(1):321-7
references: 10739680
references: Chem Rev. 2004 Aug;104(8):3623-40
references: 15303831
references: Chem Rev. 2006 Aug;106(8):3055-79
references: 16895318
references: J Biomol NMR. 2009 Aug;44(4):213-23
references: 19548092
references: Invest Ophthalmol Vis Sci. 2006 Nov;47(11):4674-82
references: 17065473
references: Exp Cell Res. 2007 Aug 15;313(14):3153-65
references: 17632102
references: Mol Cell. 2009 Apr 24;34(2):234-49
references: 19394300
references: Structure. 2005 May;13(5):703-12
references: 15893661
references: Methods Mol Biol. 2004;278:313-52
references: 15318002
references: J Biomol NMR. 2001 Dec;21(4):377-82
references: 11824758
references: Proc Natl Acad Sci U S A. 2009 Oct 20;106(42):17729-34
references: 19805198
references: Science. 2001 Oct 12;294(5541):339-45
references: 11546839
references: J Biomol NMR. 1996 Dec;8(4):477-86
references: 9008363
references: Cell. 2002 Sep 6;110(5):587-97
references: 12230976
references: Science. 2002 Apr 5;296(5565):151-5
references: 11884718
references: J Biol Chem. 1996 Mar 22;271(12):6571-4
references: 8636068
references: Exp Cell Res. 2006 Apr 1;312(6):925-37
references: 16434034
references: Biochem J. 2001 May 15;356(Pt 1):233-40
references: 11336656
references: Structure. 2010 Oct 13;18(10):1280-8
references: 20947017
references: J Biol Chem. 2004 Sep 24;279(39):40252-4
references: 15299032
references: PLoS Biol. 2004 Jun;2(6):e153
references: 15208712
references: J Mol Biol. 2003 Apr 11;327(5):1031-41
references: 12662928
references: Biochemistry. 2008 Apr 1;47(13):4008-16
references: 18321071
references: Asian J Androl. 2010 Jul;12(4):548-55
references: 20531279