title: Expression of TEX101, regulated by ACE, is essential for the production of fertile mouse spermatozoa.
abstract: Formation of spermatozoa of normal shape, number, and motility is insufficient for the male siring of pups. The spermatozoa must be accompanied by sound fertilizing ability. We found that males with disrupted testis-expressed gene 101 (Tex101) produce normal-looking but fertilization-incompetent spermatozoa, which were accompanied by a deficiency of a disintegrin and metallopeptidase domain 3 (ADAM3) on sperm plasma membrane. It was also found that the existence of TEX101 on spermatozoa was regulated by angiotensin-converting enzyme (ACE). The removal of GPI-anchored protein TEX101 by ACE was essential to produce fertile spermatozoa, and the function of ACE was not depending on its well-known peptidase activity. The finding of TEX101 as a unique specific substrate for ACE may provide a potential target for the production of an awaited contraceptive medicine for men.
authors: Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan.
authors: Yoshitaka
authors: Y
authors: Fujihara
authors: Keizo
authors: K
authors: Tokuhiro
authors: Yuko
authors: Y
authors: Muro
authors: Gen
authors: G
authors: Kondoh
authors: Yoshihiko
authors: Y
authors: Araki
authors: Masahito
authors: M
authors: Ikawa
authors: Masaru
authors: M
authors: Okabe
mesh_terms: D051722:ADAM Proteins / Q000378:metabolism; D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism*; D005260:Female; D058851:GPI-Linked Proteins / Q000378:metabolism; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D010447:Peptide Hydrolases / Q000378:metabolism; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism*; D010641:Phenotype; D013094:Spermatozoa / Q000378:metabolism*; D013737:Testis / Q000378:metabolism; D015044:Zona Pellucida / Q000378:metabolism
chemical_list: D000954:Antigens, Surface; D058851:GPI-Linked Proteins; D008562:Membrane Glycoproteins; C426726:TEX101 protein, mouse; D010447:Peptide Hydrolases; D007703:Peptidyl-Dipeptidase A; D051722:ADAM Proteins; C485429:Adam3 protein, mouse
keywords: GPIase; cyritestin; fertility; germ-cell; knockout
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