title: Determination of in vivo behavior of mitomycin C-loaded o/w soybean oil microemulsion and mitomycin C solution via gamma camera imaging.
abstract: In this study, a microemulsion system was evaluated for delivery of mitomycin C (MMC). To track the distribution of the formulated drug after intravenous administration, radiochemical labeling and gamma scintigraphy imaging were used. The aim was to evaluate a microemulsion system for intravenous delivery of MMC and to compare its in vivo behavior with that of the MMC solution. For microemulsion formulation, soybean oil was used as the oil phase. Lecithin and Tween 80 were surfactants and ethanol was the cosurfactant. To understand the whole body localization of MMC-loaded microemulsion, MMC was labeled with radioactive technetium and gamma scintigraphy was applied for visualization of drug distribution. Radioactivity in the bladder 30 minutes after injection of the MMC solution was observed, according to static gamma camera images. This shows that urinary excretion of the latter starts very soon. On the other hand, no radioactivity appeared in the urinary bladder during the 90 minutes following the administration of MMC-loaded microemulsion. The unabated radioactivity in the liver during the experiment shows that the localization of microemulsion formulation in the liver is stable. In the light of the foregoing, it is suggested that this microemulsion formulation may be an appropriate carrier system for anticancer agents by intravenous delivery in hepatic cancer chemotherapy.
authors: Department of Pharmaceutical Technology, Ege University, Izmir, Turkey.
authors: Mustafa
authors: M
authors: Kotmakçı
authors: Gülten
authors: G
authors: Kantarcı
authors: Makbule
authors: M
authors: Aşıkoğlu
authors: Hayal
authors: H
authors: Ozkılıç
authors: Gökhan
authors: G
authors: Ertan
mesh_terms: D061605:Administration, Intravenous; D000818:Animals; D004655:Emulsions / Q000008:administration & dosage / Q000737:chemistry; D015902:Gamma Cameras; D006801:Humans; D008297:Male; D016685:Mitomycin / Q000008:administration & dosage* / Q000737:chemistry* / Q000493:pharmacokinetics; D011817:Rabbits; D011877:Radionuclide Imaging / Q000379:methods*; D013670:Sodium Pertechnetate Tc 99m / Q000008:administration & dosage / Q000737:chemistry; D013024:Soybean Oil / Q000008:administration & dosage* / Q000737:chemistry* / Q000493:pharmacokinetics; D013501:Surface-Active Agents / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics
chemical_list: D004655:Emulsions; D013501:Surface-Active Agents; D016685:Mitomycin; D013024:Soybean Oil; D013670:Sodium Pertechnetate Tc 99m