title: Engineered Enterobacter aerogenes for efficient utilization of sugarcane molasses in 2,3-butanediol production.
abstract: Sugarcane molasses is considered to be a good carbon source for biorefinery due to its high sugar content and low price. Sucrose occupies more than half of the sugar in the molasses. Enterobacter aerogenes is a good host strain for 2,3-butanediol production, but its utilization of sucrose is not very efficient. To improve sucrose utilization in E. aerogenes, a sucrose regulator (ScrR) was disrupted from the genomic DNA. The deletion mutation increased the sucrose consumption rate significantly when sucrose or sugarcane molasses was used as a carbon source. The 2,3-butanediol production from sugarcane molasses by the mutant was enhanced by 60% in batch fermentation compared to that by the wild type strain. In fed-batch fermentation, 98.69 g/L of 2,3-butanediol production was achieved at 36 h.
authors: Dept. of Chemical & Biological Engineering, Korea University, Seoul 136-713, Republic of Korea.
authors: Moo-Young
authors: MY
authors: Jung
authors: Bu-Soo
authors: BS
authors: Park
authors: Jinwon
authors: J
authors: Lee
authors: Min-Kyu
authors: MK
authors: Oh
mesh_terms: D061249:Batch Cell Culture Techniques; D001709:Biotechnology / Q000379:methods*; D002072:Butylene Glycols / Q000378:metabolism*; D002241:Carbohydrates / Q000494:pharmacology; D002244:Carbon / Q000494:pharmacology; D021902:Enterobacter aerogenes / Q000187:drug effects / Q000378:metabolism*; D005285:Fermentation / Q000187:drug effects; D005818:Genetic Engineering; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D008965:Molasses; D009154:Mutation / Q000235:genetics; D031786:Saccharum / Q000187:drug effects / Q000378:metabolism*; D013395:Sucrose / Q000737:chemistry / Q000378:metabolism
chemical_list: D002072:Butylene Glycols; D002241:Carbohydrates; C026978:2,3-butylene glycol; D013395:Sucrose; D002244:Carbon