title: [Reliability study on the infrared spectrometry for measuring the delta over baseline for breath 13C].
abstract: OBJECTIVE
  To assess the validity and reliability of the infrared spectrometry for measuring the delta over baseline for breath 13C.
  
  
  METHODS
  Twenty-four healthy adults were selected, including twelve males and twelve females. 13C-Leucine was used as tracer in stable isotope metabolic experiments. One baseline breath was collected before the tracer protocol began. Other breath samples were collected at 60, 120, 180, 195, 210, 225, 240, 255, 270 and 300 min post-tracer challenge. The delta over baseline for breath 13C was measured by the infrared spectrometry and Heliview 13C breath analyzer.
  
  
  RESULTS
  No significant differences were found between the infrared spectrometry and Heliview 13C breath analyzer in measuring the delta over baseline for breath 13C. The F value and p value of two-way ANOVA were 0.29 and 0.5874 respectively. The t and p value of consistency test were 0.48 and 0.6346 respectively.
  
  
  CONCLUSIONS
  The 13C infrared spectrometry can reliably measure the delta over baseline for breath 13C.
authors: Chinese Center for Disease Control and Prevention, Beijing 102206, China. wangmincdc@163.com
authors: Min
authors: M
authors: Wang
authors: Zhiling
authors: Z
authors: Wang
authors: Lingyan
authors: L
authors: Gou
authors: Yuhui
authors: Y
authors: Zhang
authors: Xiaoguang
authors: X
authors: Yang
authors: Lei
authors: L
authors: Sha
authors: Min
authors: M
authors: Li
mesh_terms: D000328:Adult; D001944:Breath Tests / Q000379:methods*; D002247:Carbon Isotopes; D005260:Female; D006801:Humans; D007930:Leucine; D008297:Male; D015203:Reproducibility of Results; D013055:Spectrophotometry, Infrared / Q000379:methods*
chemical_list: D002247:Carbon Isotopes; D007930:Leucine