title: Tumor-derived tenascin-C promotes the epithelial-mesenchymal transition in colorectal cancer cells.
abstract: BACKGROUND
  Tenascin-C (TNC) is an extracellular matrix glycoprotein, usually derived from myofibroblasts in the cancer microenvironment. Recently, however, the significance of tumor-derived TNC in initiation of cancer metastasis was disclosed. We investigated the clinical significance of cancer-derived TNC in colorectal cancer (CRC) cases.
  
  
  METHODS
  TNC expression in 170 cases of CRC was analyzed by quantitative real-time polymerase chain reaction (PCR). In addition, gene expression arrays using purely-separated cancer tissues of another 86 cases was performed and the functional implications of cancer-specific TNC were investigated.
  
  
  RESULTS
  The expression of TNC mRNA was significantly higher in CRC tissues than in the corresponding normal tissues. Cancer cell-specific TNC expression was a significant prognostic factor in CRC cases. Moreover, cancer cell-derived TNC was associated with the epithelial-mesenchymal transition (EMT) signature.
  
  
  CONCLUSIONS
  Cancer cell-derived TNC promotes cancer invasiveness via EMT regulation, and not cancer tissue TNC but cancer cell-specific TNC is a novel indicator of poor prognosis.
authors: Department of Surgery, Beppu Hospital, Kyushu University, 4546 Tsurumihara, Beppu 874-0838, Japan.
authors: Yusuke
authors: Y
authors: Takahashi
authors: Genta
authors: G
authors: Sawada
authors: Junji
authors: J
authors: Kurashige
authors: Tae
authors: T
authors: Matsumura
authors: Ryutaro
authors: R
authors: Uchi
authors: Hiroki
authors: H
authors: Ueo
authors: Masahisa
authors: M
authors: Ishibashi
authors: Yuki
authors: Y
authors: Takano
authors: Sayuri
authors: S
authors: Akiyoshi
authors: Takeshi
authors: T
authors: Iwaya
authors: Hidetoshi
authors: H
authors: Eguchi
authors: Tomoya
authors: T
authors: Sudo
authors: Keishi
authors: K
authors: Sugimachi
authors: Hirofumi
authors: H
authors: Yamamoto
authors: Yuichiro
authors: Y
authors: Doki
authors: Masaki
authors: M
authors: Mori
authors: Koshi
authors: K
authors: Mimori
mesh_terms: D000368:Aged; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D002448:Cell Adhesion; D002465:Cell Movement; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D005500:Follow-Up Studies; D020869:Gene Expression Profiling; D006801:Humans; D007124:Immunoenzyme Techniques; D061346:Laser Capture Microdissection; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000556:secondary*; D008207:Lymphatic Metastasis; D008297:Male; D009361:Neoplasm Invasiveness; D020411:Oligonucleotide Array Sequence Analysis; D010534:Peritoneal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000556:secondary*; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology; D019063:Tenascin / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D019063:Tenascin
keywords: EMT; TNC; Tenascin-C; colorectal cancer