title: Overexpression of long noncoding RNA PCAT-1 is a novel biomarker of poor prognosis in patients with colorectal cancer.
abstract: Long noncoding RNAs (lncRNA) are emerging as key molecules in human cancer. Prostate cancer-associated ncRNA transcripts 1 (PCAT-1), a lncRNA, has been recently revealed involving in human prostate cancer progression. However, whether PCAT-1 could serve as novel biomarker to predict prognosis in colorectal cancer (CRC) or not is unknown. We therefore carried out the present study to explore the correlation between PCAT-1 expression and the progression of CRC. In this study, the expression of PCAT-1 in 108 cases of CRC tissues and matched 81 adjacent normal tissues were determined by quantitative real-time PCR. Furthermore, the copy number variation of PCAT-1 was also measured in 17 tumor tissues and matched normal tissues. Our results showed that PCAT-1 expression in CRC tissues was significantly upregulated compared with the matched normal tissues (p < 0.001) and the overexpression of PCAT-1(upregulated by more than 50 %) was found in 64 % (62/81) of CRC. Moreover, PCAT-1 gene copy number variation explains only a few percent of observed overexpression. In addition, there was a significant association between PCAT-1 expression and distant metastasis (p = 0.04), but not other clinical characteristics. More important, CRC patients with PCAT-1 higher expression have shown significantly poorer overall survival than those with lower PCAT-1 expression (p < 0.001). Also, multivariable Cox regression analysis identified PCAT-1 overexpression as an independent prognostic factor for CRC (p = 0.007, HR = 3.12 95 %CI = 1.355-7.185). In conclusion, our results suggest that high expression of PCAT-1 is involved in CRC progression and could be a novel biomarker of poor prognosis in patient with colorectal cancer.
authors: State Key Laboratory of Oncology in South China, Sun Yat-Sen University Cancer Center, Guangzhou, China.
authors: Xiaosong
authors: X
authors: Ge
authors: Yuanbin
authors: Y
authors: Chen
authors: Xiaoyu
authors: X
authors: Liao
authors: Deqing
authors: D
authors: Liu
authors: Fangfang
authors: F
authors: Li
authors: Honglian
authors: H
authors: Ruan
authors: Weihua
authors: W
authors: Jia
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D062085:RNA, Long Noncoding / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012307:Risk Factors
chemical_list: D014408:Biomarkers, Tumor; D062085:RNA, Long Noncoding; D012333:RNA, Messenger
references: Sci Transl Med. 2012 Mar 28;4(127):127rv3
references: 22461644
references: Anticancer Res. 2006 Sep-Oct;26(5A):3439-44
references: 17094464
references: Cancer Res. 2011 Aug 15;71(16):5365-9
references: 21828241
references: Cell. 2010 Aug 6;142(3):409-19
references: 20673990
references: J Cell Physiol. 2013 Apr;228(4):764-72
references: 23001792
references: CA Cancer J Clin. 2012 Jan-Feb;62(1):10-29
references: 22237781
references: Clin Cancer Res. 2006 Apr 1;12(7 Pt 1):2125-32
references: 16609025
references: Nat Genet. 2011 Jun 28;43(7):616-7
references: 21709680
references: J Urol. 2012 Mar;187(3):795-801
references: 22245323
references: PLoS One. 2011;6(7):e21651
references: 21765901
references: Nat Rev Genet. 2008 Feb;9(2):102-14
references: 18197166
references: Genes Dev. 2009 Jul 1;23(13):1494-504
references: 19571179
references: Am J Transl Res. 2012;4(2):127-50
references: 22611467
references: Hepatology. 2013 May;57(5):1882-92
references: 23239537
references: Nat Biotechnol. 2011 Jul 31;29(8):742-9
references: 21804560
references: Nature. 2010 Apr 15;464(7291):1071-6
references: 20393566
references: Ann Surg Oncol. 2011 May;18(5):1243-50
references: 21327457
references: Oncogene. 2011 Nov 24;30(47):4750-6
references: 21625215
references: Clin Cancer Res. 2002 May;8(5):1248-52
references: 12006545
references: Clin Cancer Res. 2006 May 1;12(9):2795-803
references: 16675573
references: Cell. 2011 Apr 15;145(2):178-81
references: 21496640
references: Clin Cancer Res. 2001 Aug;7(8):2213-21
references: 11489794
references: Cold Spring Harb Perspect Biol. 2010 Sep;2(9):a003749
references: 20739414
references: Med Oncol. 2012 Sep;29(3):1810-6
references: 21678027
references: Nature. 2011 Aug 28;477(7364):295-300
references: 21874018
references: Oncogene. 2012 Oct 25;31(43):4577-87
references: 22266873