title: Engineering bone tissue using human dental pulp stem cells and an osteogenic collagen-hydroxyapatite-poly (L-lactide-co-ε-caprolactone) scaffold.
abstract: The aim of this study was to design a new natural/synthetic bioactive bone scaffold for potential use in bone replacement applications. We developed a tri-component osteogenic composite scaffold made of collagen (Coll), hydroxyapatite (HA) and poly(l-lactide-co-ε-caprolactone) (PLCL). This Coll/HA/PLCL composite scaffold was combined with human osteoblast-like cells obtained by differentiation of dental pulp stem cells (DPSCs) to engineer bone tissue in vitro. Results show that the 3D Coll/HA/PLCL composite scaffold was highly porous, thereby enabling osteoblast-like cell adhesion and growth. Cultured in the Coll/HA/PLCL scaffold, the osteoblast-like cells expressed different osteogenic genes, produced alkaline phosphatase and formed nodules more than did PLCL alone. Micro-CT analyses revealed a significant (30%) increase of tissue mineralisation on the surface as well as inside of the Coll/HA/PLCL scaffold, thus confirming its effectiveness as a bone regeneration platform.
authors: 1Groupe de recherche en écologie buccale, Faculty of Dentistry, Laval University, Quebec, Canada.
authors: Adil
authors: A
authors: Akkouch
authors: Ze
authors: Z
authors: Zhang
authors: Mahmoud
authors: M
authors: Rouabhia
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001483:Base Sequence; D001846:Bone Development; D003094:Collagen; D017931:DNA Primers; D003782:Dental Pulp / Q000166:cytology*; D017886:Durapatite; D006801:Humans; D008855:Microscopy, Electron, Scanning; D011091:Polyesters; D060888:Real-Time Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology*; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D055114:X-Ray Microtomography
chemical_list: D017931:DNA Primers; D011091:Polyesters; C553659:polycaprolactone-co-lactide; D003094:Collagen; D017886:Durapatite; D000469:Alkaline Phosphatase
keywords: Human dental pulp stem cells; biomaterial; bone regeneration; collagen; differentiation; hydroxyapatite; poly(lactide-co-ε-caprolactone)