title: Zebrafish scales respond differently to in vitro dynamic and static acceleration: analysis of interaction between osteoblasts and osteoclasts.
abstract: Zebrafish scales consist of bone-forming osteoblasts, bone-resorbing osteoclasts, and calcified bone matrix. To elucidate the underlying molecular mechanism of the effects induced by dynamic and static acceleration, we investigated the scale osteoblast- and osteoclast-specific marker gene expression involving osteoblast-osteoclast communication molecules. Osteoblasts express RANKL, which binds to the osteoclast surface receptor, RANK, and stimulates bone resorption. OPG, on the other hand, is secreted by osteoblast as a decoy receptor for RANKL, prevents RANKL from binding to RANK and thus prevents bone resorption. Therefore, the RANK-RANKL-OPG pathway contributes to the regulation of osteoclastogenesis by osteoblasts. Semaphorin 4D, in contrast, is expressed on osteoclasts, and binding to its receptor Plexin-B1 on osteoblasts results in suppression of bone formation. In the present study, we found that both dynamic and static acceleration at 3.0×g decreased RANKL/OPG ratio and increased osteoblast-specific functional mRNA such as alkaline phosphatase, while static acceleration increased and dynamic acceleration decreased osteoclast-specific mRNA such as cathepsin K. Static acceleration increased semaphorin 4D mRNA expression, while dynamic acceleration had no effect. The results of the present study indicated that osteoclasts have predominant control over bone metabolism via semaphorin 4D expression induced by static acceleration at 3.0×g.
authors: Faculty of Health Sciences, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kanazawa, Ishikawa 920-0942, Japan. kkitamur@staff.kanazawa-u.ac.jp
authors: Kei-ichiro
authors: K
authors: Kitamura
authors: Koh
authors: K
authors: Takahira
authors: Masato
authors: M
authors: Inari
authors: Yusuke
authors: Y
authors: Satoh
authors: Kazuichi
authors: K
authors: Hayakawa
authors: Yoshiaki
authors: Y
authors: Tabuchi
authors: Kazuhiro
authors: K
authors: Ogai
authors: Takumi
authors: T
authors: Nishiuchi
authors: Takashi
authors: T
authors: Kondo
authors: Yuko
authors: Y
authors: Mikuni-Takagaki
authors: Wenxi
authors: W
authors: Chen
authors: Atsuhiko
authors: A
authors: Hattori
authors: Nobuo
authors: N
authors: Suzuki
mesh_terms: D000054:Acceleration; D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000825:Animal Structures / Q000166:cytology* / Q000378:metabolism*; D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D010006:Osteoblasts / Q000378:metabolism*; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D010010:Osteoclasts / Q000378:metabolism*; D053244:Osteoprotegerin / Q000235:genetics / Q000378:metabolism; D053245:RANK Ligand / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D039961:Semaphorins / Q000235:genetics / Q000378:metabolism; D015027:Zebrafish / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; C093224:CD100 antigen; D024042:Collagen Type I; D053244:Osteoprotegerin; D053245:RANK Ligand; D012333:RNA, Messenger; D039961:Semaphorins; D029961:Zebrafish Proteins; D015675:Osteocalcin; D000469:Alkaline Phosphatase
keywords: ALP; Bone metabolism; Cell-to-cell communication; DCSTAMP; Dlx5; NFATC1; OPG; RANK; RANK ligand; RANKL; Semaphorin 4D; Static and dynamic acceleration; Trap; Zebrafish scale; alkaline phosphatase; dendritic cell-specific transmembrane protein; distal-less homeobox protein 5; g; gravitational acceleration; nuclear factor of activated T cells, cytoplasmic 1; osteoprotegerin; receptor activator of nuclear factor kappa-B; tartrate-resistant acid phosphatase