title: Narrowband ultraviolet B interferes with gene expression in the peripheral blood T cells of patients with psoriasis.
abstract: BACKGROUND
  Psoriasis pathogenesis and development are closely related to abnormal T cell activity. Narrowband ultraviolet B (NB-UVB) treatment markedly improves skin lesions in psoriasis.
  
  
  OBJECTIVE
  To investigate differential gene expression in psoriasis and to understand the possible mechanisms of NB-UVB therapy for psoriasis.
  
  
  METHODS
  The mRNA expression profiles and differentially expressed genes from peripheral blood T cells of psoriatic patients before and after NB-UVB treatment were examined using RNA sequencing and validated by real-time reverse-transcription polymerase chain reaction.
  
  
  RESULTS
  A total of 129 genes were differentially expressed in the peripheral blood T cells of psoriatic patients: 83 genes were downregulated and 46 were upregulated in psoriatic patients compared to those of healthy subjects. These genes were enriched in intracellular membrane-bound organelles, membrane-bound organelles and the nucleus, and are involved in the cell cycle, apoptosis, inflammation and other processes. These changes are reversed in psoriatic patients with good clinical outcomes following NB-UVB treatment.
  
  
  CONCLUSIONS
  NB-UVB treatment has beneficial effects on local psoriatic lesions, possibly due to its effect on peripheral blood T cell gene expression.
authors: Institute of Dermatology, Taiyuan City Center Hospital, Shanxi Medical University, Taiyuan, China.
authors: Junqin
authors: J
authors: Li
authors: Ruixia
authors: R
authors: Hou
authors: Yuanwen
authors: Y
authors: Yang
authors: Ruifeng
authors: R
authors: Liu
authors: Xincheng
authors: X
authors: Zhao
authors: Xinhua
authors: X
authors: Li
authors: Guohua
authors: G
authors: Yin
authors: Peng
authors: P
authors: An
authors: Yong
authors: Y
authors: Wang
authors: Kaiming
authors: K
authors: Zhang
mesh_terms: D000328:Adult; D001483:Base Sequence; D005260:Female; D015870:Gene Expression / Q000528:radiation effects*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011565:Psoriasis / Q000097:blood* / Q000235:genetics* / Q000532:radiotherapy; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000528:radiation effects*; D014466:Ultraviolet Rays; D014467:Ultraviolet Therapy
chemical_list: D012333:RNA, Messenger