title: Dual-fluorescent RNA probes with an extremely large stokes shift.
abstract: Fluorescent probes are powerful and indispensable tools for imaging RNA in vivo and in vitro. To simultaneously visualize multiple RNA targets in a cell, it is necessary to develop probes which emit fluorescence with different colors by excitation at a single wavelength. We synthesized OMUpy1 and OMUpy2 in this study with a cyanine dye respectively conjugated at their 5' ends. A fluorescent analysis revealed these probes to have yellow or pink fluorescence derived from the cyanine dyes with an extremely large Stokes shift. Three color-coded fluorescent images were also obtained in the presence of target RNAs.
authors: Kyoto Institute of Technology, Graduate School of Science and Technology, Department of Biomolecular Engineering, Goshokaida-cho, Matsugasaki, Sakyo-ku, Kyoto, Japan. akobori@kit.ac.jp
authors: Akio
authors: A
authors: Kobori
authors: Takako
authors: T
authors: Ueda
authors: Yuya
authors: Y
authors: Sanada
authors: Asako
authors: A
authors: Yamayoshi
authors: Akira
authors: A
authors: Murakami
mesh_terms: D002232:Carbocyanines / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D009843:Oligoribonucleotides / Q000737:chemistry; D012313:RNA / Q000737:chemistry*
chemical_list: D002232:Carbocyanines; D005456:Fluorescent Dyes; D009843:Oligoribonucleotides; D012313:RNA