title: Immunomodulatory effects of the botanical compound LCS101: implications for cancer treatment.
abstract: OBJECTIVE
  To examine the effects of LSC101, a botanical compound, on adaptive and innate immunity.
  
  
  METHODS
  LCS101 preparations were tested for batch-to-batch consistency using high-performance liquid chromatography. T-cell activation was quantified in murine spleen cells using 3H-thymidine incorporation, and cytokine production analyzed with enzyme-linked immunosorbent assay. Natural killer cell activity was tested on human blood cells using flow cytometry, and cytotoxicity measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and apoptosis using a FACSCalibur. Effects on interferon-γ production in fluorouracil/doxorubicin-treated mice were tested with enzyme-linked immunosorbent assay.
  
  
  RESULTS
  High-performance liquid chromatography analysis demonstrated batch-to-batch consistency. T-cell proliferation was increased, and a dose-dependent activation of natural killer cells and macrophage tumor necrosis factor-α secretion were observed with LCS101 treatment. Interferon-γ levels, reduced following fluorouracil treatment, were corrected in treated animals. No toxicity or compromised treatment outcomes were associated with LCS101 exposure.
  
  
  CONCLUSIONS
  LCS101 demonstrated significant effects on a number of immune processes. Further research is needed in order to understand the molecular immunomodulatory pathways affected by this compound, as well as clinical implications for treatment.
authors: The Gonda-Goldschmied Center, Bar-Ilan University, Ramat-Gan, Israel.
authors: Itzchak H
authors: IH
authors: Rachmut
authors: Noah
authors: N
authors: Samuels
authors: Steven J
authors: SJ
authors: Melnick
authors: Cheppail
authors: C
authors: Ramachandran
authors: Yedida
authors: Y
authors: Sharabi
authors: Anya
authors: A
authors: Pavlovsky
authors: Yair
authors: Y
authors: Maimon
authors: Jacob
authors: J
authors: Shoham
keywords: NK cells; T-cell immunity; TNF-alpha; botanical compound; immunomodulation; interferon-gamma
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