title: The Accumulation of Crocin and Geniposide and Transcripts of Phytoene Synthase during Maturation of Gardenia jasminoides Fruit.
abstract: Gardenia fruit (fruit of Gardenia jasminoides Ellis) is used as a natural pigment resource and a Chinese traditional medicine. The white mesocarp turning orange or red that occurs during gardenia fruit maturation arises from the production and accumulation of the apocarotenoids, especially crocin-1, which is derived from carotenoid. Meanwhile, the major medical component geniposide is accumulated in gardenia fruit. To further our understanding of the synthetic and accumulation mechanism for crocin-1 and geniposide in gardenia fruit, the contents of crocin-1 and geniposide and the transcripts of phytoene synthase (GjPSY) profiles in gardenia fruits were examined at various stages of maturation. The concentration of crocin-1 and geniposide in gardenia fruit was determined by reversed-phase high-performance liquid chromatography (HPLC). The results showed that the concentration of crocetin-1 was increased during fruit development and the concentration of geniposide does not change significantly during maturing. The expression levels of GjPSY mRNA were examined by RT-PCR. It was revealed that GjPSY was constitutively expressed during fruit development, suggesting that the primary mechanism that controls crocin accumulation in G. jasminoides fruits during development is not correlated to the differential regulation of transcript levels of GjPSY gene.
authors: School of Basic Courses of Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou, Guangdong 510006, China.
authors: Lan
authors: L
authors: Gao
authors: Bi-Yun
authors: BY
authors: Zhu
references: J Cardiovasc Pharmacol. 2006 Jan;47(1):70-6
references: 16424788
references: Br J Pharmacol. 2008 Jul;154(5):1016-24
references: 18469847
references: Plant Cell. 2003 Jan;15(1):47-62
references: 12509521
references: Cell Mol Neurobiol. 2012 Mar;32(2):227-35
references: 21901509
references: J Biol Chem. 2011 Sep 16;286(37):32866-74
references: 21799001
references: Eur J Pharmacol. 2011 Jan 10;650(1):110-9
references: 20951131
references: J Agric Food Chem. 2007 Feb 7;55(3):970-7
references: 17263501
references: Oncol Rep. 2012 Feb;27(2):567-72
references: 22020372
references: Plant Physiol. 2004 Feb;134(2):824-37
references: 14739348
references: Nutr Res. 2009 Mar;29(3):145-50
references: 19358927
references: Plant Cell. 2010 Oct;22(10):3348-56
references: 20889914
references: Biol Pharm Bull. 2005 Nov;28(11):2106-10
references: 16272698
references: Biol Pharm Bull. 2011;34(10):1613-8
references: 21963504
references: Plant Cell. 2007 Oct;19(10):3194-211
references: 17933904
references: Zhong Yao Cai. 2000 Dec;23(12):754-6
references: 12575268
references: FEBS Lett. 2012 Apr 5;586(7):1055-61
references: 22569263
references: Chem Pharm Bull (Tokyo). 2000 May;48(5):746-8
references: 10823717
references: Mol Cancer Ther. 2009 Feb;8(2):315-23
references: 19208826
references: Asian Pac J Cancer Prev. 2011;12(10):2679-83
references: 22320974
references: Biomacromolecules. 2008 Nov;9(11):3127-32
references: 18942790
references: Planta. 2005 Jun;221(3):305-8
references: 15834583
references: Saudi J Gastroenterol. 2009 Jul-Sep;15(3):181-7
references: 19636180
references: Biol Pharm Bull. 2010;33(11):1841-6
references: 21048309
references: Chin Med J (Engl). 2009 Dec 5;122(23):2886-92
references: 20092796
references: Acta Pharmacol Sin. 2009 Feb;30(2):202-8
references: 19122671
references: Fitoterapia. 2010 Jun;81(4):269-73
references: 19815056
references: J Pharm Pharmacol. 2011 Apr;63(4):587-93
references: 21401612
references: Phytomedicine. 2010 Sep;17(11):840-3
references: 20537515
references: Plant Physiol. 2005 Oct;139(2):674-89
references: 16183835