title: Molecular Typing of Canine parvovirus Occurring in Pondicherry by Multiplex PCR and PCR-RFLP.
abstract: The present study was aimed at molecular typing of Canine parvovirus (CPV) occurring in Pondicherry using PCR based assays. CPV-2a and CPV-2b types were detected by PCR in 68 (53.12%) out of 128 faecal samples/rectal swabs. All the 68 samples found positive by PCR assay were subjected to multiplex PCR assay with CPV-2ab and CPV-2b primer pairs. Sixty-seven (98.52%) samples were characterized as CPV-2b type and one sample (1.47%) was categorized as CPV-2a type. Sixty clinical samples found negative by CPV-2ab primers were subjected to another PCR assay with CPV-555 primer pair for detecting CPV-2c. Though three samples (5%) responded to this PCR, subsequent RFLP of these PCR products with MboII did not show any cleavage indicating the absence of CPV-2c in Pondicherry. It was inferred that CPV-2b was the most prevalent CPV type in Pondicherry. It was further concluded that the CPV-2 variants (CPV-2a, CPV-2b and CPV-2c) currently circulating in the field worldwide could be diagnosed by employing multiplex PCR and PCR-RFLP assays.
authors: Rajiv Gandhi College of Veterinary and Animal Sciences, 10/442, 1st Cross, Thulukathamman Nagar, Murungapakkam Post, Pondicherry, 605004 India.
authors: S
authors: S
authors: Parthiban
authors: H K
authors: HK
authors: Mukhopadhyay
authors: P X
authors: PX
authors: Antony
authors: R M
authors: RM
authors: Pillai
keywords: CPV-2a; CPV-2b; CPV-2c; Multiplex PCR; PCR; PCR–RFLP
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