title: Immature and mature dengue serotype 1 virus structures provide insight into the maturation process.
abstract: Dengue virus is a major human pathogen that has four serotypes (DENV1 to -4). Here we report the cryoelectron microscopy (cryo-EM) structures of immature and mature DENV1 at 6- and 4.5-Å resolution, respectively. The subnanometer-resolution maps allow accurate placement of all of the surface proteins. Although the immature and mature viruses showed vastly different surface protein organizations, the envelope protein transmembrane (E-TM) regions remain in similar positions. The pivotal role of the E-TM regions leads to the identification of the start and end positions of all surface proteins during maturation.
authors: Program in Emerging Infectious Diseases, Duke-NUS Graduate Medical School, Singapore.
authors: Victor A
authors: VA
authors: Kostyuchenko
authors: Qian
authors: Q
authors: Zhang
authors: Joanne L
authors: JL
authors: Tan
authors: Thiam-Seng
authors: TS
authors: Ng
authors: Shee-Mei
authors: SM
authors: Lok
mesh_terms: D020285:Cryoelectron Microscopy; D003716:Dengue Virus / Q000235:genetics* / Q000254:growth & development* / Q000648:ultrastructure*; D008958:Models, Molecular; D011487:Protein Conformation; D012703:Serotyping; D014764:Viral Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D014764:Viral Proteins
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