title: Cell proliferation and cell sheet detachment from the positively and negatively charged nanocomposite hydrogels.
abstract: The charged nanocomposite hydrogels (NC gels) were synthesized by copolymerization of positively or negatively chargeable monomer with N-isopropylacrylamide (NIPAm) in the aqueous suspension of hectorite clay. The ionic NC gels preserved the thermo-responsibility with the phase-transition temperature below 37°C. The L929 cell proliferation was sensitive to charge polarity and charge density. As compared to the PNIPAm NC gel, the cationic NC gels with <5 mol % of 2-(dimethylamino)ethyl methacrylate (DMAEMA) showed improved cell proliferation, whereas the cells grew slowly on the gels with negatively charged 2-acrylamido-2-methylpropane sulfonic acid (AMPSNa). By lowering temperature, rapid cell sheet detachment was observed from the surface of ionic NC gels with 1 mol % of ionizable monomers. However, lager amount of AMPSNa or DMAEMA did not support rapid cell sheet detachment, probably owing to the adverse swelling effects and/or enhanced electrostatic attraction.
authors: Research Institute of Materials Science, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou, 510640, People's Republic of China.
authors: Dan
authors: D
authors: Liu
authors: Tao
authors: T
authors: Wang
authors: Xinxing
authors: X
authors: Liu
authors: Zhen
authors: Z
authors: Tong
mesh_terms: D049109:Cell Proliferation; D020100:Hydrogels; D053761:Nanocomposites; D011108:Polymers; D013696:Temperature
chemical_list: D020100:Hydrogels; D011108:Polymers
keywords: cell culture; cell sheet detachment; nanocomposite hydrogels; negatively charged gel; positively charged gel