title: Effects of nanoencapsulation and PEGylation on biodistribution of indocyanine green in healthy mice: quantitative fluorescence imaging and analysis of organs.
abstract: Near-infrared nanoconstructs present a potentially effective platform for site-specific and deep tissue optical imaging and phototherapy. We have engineered a polymeric nanocapsule composed of polyallylamine hydrochloride (PAH) chains cross-linked with sodium phosphate and doped with indocyanine green (ICG) toward such endeavors. The ICG-doped nanocapsules were coated covalently with polyethylene glycol (5000 daltons) through reductive amination. We administrated the constructs by tail vein injection to healthy mice. To characterize the biodistribution of the constructs, we performed in vivo quantitative fluorescence imaging and subsequently analyzed the various extracted organs. Our results suggest that encapsulation of ICG in these PEGylated constructs is an effective approach to prolong the circulation time of ICG and delay its hepatic accumulation. Increased bioavailability of ICG, due to encapsulation, offers the potential of extending the clinical applications of ICG, which are currently limited due to rapid elimination of ICG from the vasculature. Our results also indicate that PAH and ICG-doped nanocapsules (ICG-NCs) are not cytotoxic at the levels used in this study.
authors: Department of Bioengineering, University of California, Riverside, CA 92521, USA.
authors: Baharak
authors: B
authors: Bahmani
authors: Christian Y
authors: CY
authors: Lytle
authors: Ameae M
authors: AM
authors: Walker
authors: Sharad
authors: S
authors: Gupta
authors: Valentine I
authors: VI
authors: Vullev
authors: Bahman
authors: B
authors: Anvari
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D006801:Humans; D007208:Indocyanine Green / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics*; D051379:Mice; D053769:Nanocapsules / Q000008:administration & dosage / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000737:chemistry*; D014018:Tissue Distribution; D051598:Whole Body Imaging / Q000379:methods*
chemical_list: D053769:Nanocapsules; D011092:Polyethylene Glycols; D007208:Indocyanine Green
keywords: cancer; fluorescent imaging; nanoprobes; near infrared; pharmacokinetics; phototherapy; vascular imaging
references: Breast Cancer. 2005;12(3):211-5
references: 16110291
references: Nat Biotechnol. 2004 Jan;22(1):93-7
references: 14661026
references: Ann Surg Oncol. 2012 Dec;19(13):4104-11
references: 22752379
references: Mol Pharm. 2012 Aug 6;9(8):2146-55
references: 22668197
references: Small. 2012 Sep 24;8(18):2856-68
references: 22744832
references: J Dermatol. 2012 Apr;39(4):336-8
references: 21933261
references: J Am Chem Soc. 2010 Feb 17;132(6):1929-38
references: 20092330
references: Nat Nanotechnol. 2007 Dec;2(12):751-60
references: 18654426
references: Int J Pharm. 2006 Feb 3;308(1-2):200-4
references: 16386861
references: Mol Pharm. 2008 Jul-Aug;5(4):505-15
references: 18672949
references: J Biomed Opt. 2007 Nov-Dec;12(6):064031
references: 18163847
references: Br J Surg. 1999 Aug;86(8):1005-11
references: 10460634
references: Int J Pharm. 2012 Oct 15;436(1-2):25-31
references: 22692077
references: Eur J Pharm Sci. 2006 Jan;27(1):27-36
references: 16150582
references: Biomacromolecules. 2012 Mar 12;13(3):927-36
references: 22324307
references: Curr Opin Chem Biol. 2002 Oct;6(5):642-50
references: 12413549
references: Mol Pharm. 2009 Sep-Oct;6(5):1321-32
references: 19799463
references: J Thorac Cardiovasc Surg. 2009 Jul;138(1):133-40
references: 19577070
references: Lasers Surg Med. 1999;24(1):61-8
references: 10037353
references: Biomaterials. 2012 Aug;33(22):5603-9
references: 22575835
references: Ann Surg Oncol. 2008 Jun;15(6):1640-3
references: 18379850
references: Colloids Surf B Biointerfaces. 2000 Oct 1;18(3-4):301-313
references: 10915952
references: Langmuir. 2012 Mar 20;28(11):5059-69
references: 22364506
references: Plast Reconstr Surg. 2011 May;127(5):1979-1986
references: 21532424
references: Am J Ophthalmol. 2011 May;151(5):745-751.e1
references: 21501704
references: Am J Physiol Lung Cell Mol Physiol. 2008 Sep;295(3):L400-11
references: 18641236
references: Opt Express. 2008 Dec 8;16(25):20577-87
references: 19065196
references: J Biomater Sci Polym Ed. 1999;10(10):1125-47
references: 10591136
references: Am J Ophthalmol. 2009 Dec;148(6):932-8.e1
references: 19800613
references: Toxicol Pathol. 2006;34(5):455-65
references: 17067939
references: Nat Rev Immunol. 2005 Aug;5(8):606-16
references: 16056254
references: Plast Reconstr Surg. 2011 Oct;128(4):941-947
references: 21681123
references: J Biomed Opt. 2005 Sep-Oct;10(5):054010
references: 16292970
references: Ann Surg Oncol. 2012 Dec;19(13):4112-6
references: 22782671
references: Endoscopy. 2003 Jan;35(1):36-42
references: 12510224
references: Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2010 Sep-Oct;2(5):461-77
references: 20135691
references: Curr Opin Chem Biol. 2003 Oct;7(5):626-34
references: 14580568
references: Trends Mol Med. 2010 Dec;16(12):574-83
references: 20870460
references: J Biomed Opt. 2011 Jun;16(6):066003
references: 21721804
references: Eur Radiol. 2003 Jan;13(1):195-208
references: 12541130
references: J Biomed Opt. 2011 May;16(5):051303
references: 21639563
references: Invest Ophthalmol Vis Sci. 1998 Jun;39(7):1286-90
references: 9620093
references: J Biomed Opt. 2008 Jan-Feb;13(1):014025
references: 18315383
references: Br J Surg. 2004 May;91(5):575-9
references: 15122608
references: Biotechnol Prog. 2007 Nov-Dec;23(6):1431-40
references: 17914861
references: Curr Opin Ophthalmol. 2011 May;22(3):166-73
references: 21427570
references: J Biol Chem. 2001 Mar 30;276(13):9626-30
references: 11134001
references: Surv Ophthalmol. 2000 Jul-Aug;45(1):15-27
references: 10946079
references: Bioconjug Chem. 2004 Jan-Feb;15(1):79-86
references: 14733586
references: Intensive Care Med. 2004 Mar;30(3):506-9
references: 14722640
references: Ann Biomed Eng. 2009 Jun;37(6):1190-205
references: 19308733