title: [Influence of protamine on expression of tight junction proteins in Caco-2].
abstract: Action of polycation protein protamine on the expression of tight junction proteins (claudins-1, -2, -3 and occludin) which contribute to paracellular transport function was investigated on cellular models of tight (MDCK I cell line) and leaky (Caco-2 cell line) epithelium. The expression of claudins-1,-3 and occludin was observed in both cell lines by methods of immunocytochemistry. Influence of protamine (100 microg/ml; 30 min; apical) on fluorescence intensity of claudins-1, -3 was different in MDCK I and Caco-2 cells. Addition ofprotamine to the incubation medium of Caco-2 cells resulted in significant increase of claudin-3 expression by 45 % (p <0.01) in comparison with control, whereas claudin-1 and occludin expression did not alter. On the contrary, in MDCK I cells protamine induced the significant decrease ofclaudin-1 and -3 expression by 25 % (p <0.001) and 15 % (p < 0.01) respectively, whereas occludin expression did not alter. It was confirmed by the methods of confocal laser scanning microscopy that protamine alter the expression of claudins-1, -3 directly in the tight junctions. Our results suggest that charged chyme components may alter paracellular permeability of epithelium.
authors: O N
authors: ON
authors: Vishnevskaia
authors: D V
authors: DV
authors: Burdin
authors: A N
authors: AN
authors: Gorshkov
authors: N M
authors: NM
authors: Grefner
authors: A G
authors: AG
authors: Markov
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D062445:Claudin-1 / Q000235:genetics* / Q000378:metabolism; D062446:Claudin-2 / Q000235:genetics* / Q000378:metabolism; D062465:Claudin-3 / Q000235:genetics* / Q000378:metabolism; D004285:Dogs; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D061985:Madin Darby Canine Kidney Cells; D018613:Microscopy, Confocal; D062793:Occludin / Q000235:genetics* / Q000378:metabolism; D009928:Organ Specificity; D010539:Permeability; D011479:Protamines / Q000494:pharmacology*; D055672:Static Electricity; D019108:Tight Junctions / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D062445:Claudin-1; D062446:Claudin-2; D062465:Claudin-3; D062793:Occludin; D011479:Protamines