title: Preparation of Fab-fragments of GD2-specific antibodies and analysis of their antitumor activity in vitro.
abstract: Monoclonal antibodies ME361 specific to ganglioside GD2 were isolated from the conditioned medium of hybridoma HB9326 and mouse ascitic fluid by the method of affinity chromatography; their Fab-fragments were obtained by proteolytic cleavage with papain. Evaluation of Fab-fragment specificity by flow cytometry and dot-blot analysis showed that binding effectiveness of fragments with antigens was close to that for the full-length molecule of antigen. It was shown that Fab-fragments and whole antibodies ME361 dose-dependently inhibit the proliferation of cells of mice T-lymphoma EL-4, and induce apoptosis of these cells 24 h after incubation.
authors: M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Russia.
authors: I I
authors: II
authors: Doronin
authors: I V
authors: IV
authors: Kholodenko
authors: I M
authors: IM
authors: Molotkovskaya
authors: R V
authors: RV
authors: Kholodenko
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000302:isolation & purification / Q000627:therapeutic use*; D017209:Apoptosis; D001202:Ascitic Fluid / Q000276:immunology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005732:Gangliosides / Q000276:immunology*; D006825:Hybridomas / Q000276:immunology; D007140:Immunoglobulin Fab Fragments / Q000276:immunology*; D016399:Lymphoma, T-Cell / Q000276:immunology / Q000473:pathology* / Q000628:therapy; D051379:Mice
chemical_list: D000911:Antibodies, Monoclonal; D005732:Gangliosides; D007140:Immunoglobulin Fab Fragments; C019403:ganglioside, GD2