title: Determination of isoniazid concentration in rabbit vertebrae by isotope tracing technique in conjunction with HPLC.
abstract: Medications compounded with isoniazid (INH) are usually applied to surgical sites at the completion of surgery to locally kill postoperative residual tubercle bacilli. However, the distribution and elimination of INH in the vertebrae in vivo are not known. In this study, isotope tracing was used in conjunction with high-pressure liquid chromatography (HPLC) to address this. INH and technetium-99 m-labeled INH were applied to the vertebrae of rabbits. After 2 and 6 h, osseous tissues containing INH, as determined by radionuclide imaging, were collected for detection with HPLC. The results showed that INH mainly stayed around the vertebrae 6 h after its application and did not permeate widely into the blood or other organs, except for the kidneys. The standard deviations of INH concentrations in the technetium-99 m-INH group were approximately four-fold smaller than those in the INH group. This method of coupling isotope tracing and HPLC can effectively limit experimental error during sample collection, allowing accurate and reliable identification of the concentration levels of INH in osseous tissues in vivo.
authors: Department of Spinal Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China.
authors: Peng
authors: P
authors: Liu
authors: Zhaozong
authors: Z
authors: Fu
authors: Jianming
authors: J
authors: Jiang
authors: Liang
authors: L
authors: Yuan
authors: Zhen
authors: Z
authors: Lin
mesh_terms: D000818:Animals; D000995:Antitubercular Agents / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D007538:Isoniazid / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D016014:Linear Models; D008297:Male; D011817:Rabbits; D011877:Radionuclide Imaging; D013131:Spine / Q000737:chemistry / Q000000981:diagnostic imaging*; D013667:Technetium / Q000032:analysis* / Q000097:blood / Q000493:pharmacokinetics; D014018:Tissue Distribution; D051598:Whole Body Imaging
chemical_list: D000995:Antitubercular Agents; D013667:Technetium; D007538:Isoniazid
keywords: HPLC; drug metabolism; isoniazid; spinal tuberculosis; technetium-99