title: Comparative bio-effects of SiO2/Gd2O3 nanoparticles depending on their core-shell structures.
abstract: Engineered nanoparticles (NPs) can produce negative impacts on human health even in low doses. In this study, Gd2O3 nanoparticles were synthesized via the polyol route and coated with silicon to form particles with different sizes. Although all the SiO2/Gd2O3 NPs did not induce obvious cell death at 10 microg/mL on a macrophage-like cell line (RAW 264.7), their DNA damaging effects, which is measured by single cell gel electrophoresis (SCGE), is prominent in cells treated with the sample with an overall size of 120 nm. Besides, the NPs with core size of 20 nm induced greater damaging effect than NPs with similar total size but core size of 50 nm. Further test applying the modified SCGE with endonuclease III indicated that the DNA damaging effect was greatly associated with oxide pyridine after the treatment. Using immunoblotting, we found that p53 protein is activated and accumulated after the damage occurred. These results provide a new insight into the adverse effect of nanoparticles with core-shell structures, that not only the overall size but the core size of the particles determines their effect in cells.
authors: CAS Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China.
authors: Mingyi
authors: M
authors: Zhang
authors: Lin
authors: L
authors: Xia
authors: Zhanjun
authors: Z
authors: Gu
authors: Gengmei
authors: G
authors: Xing
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D002460:Cell Line; D004249:DNA Damage; D005682:Gadolinium / Q000737:chemistry*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; C030581:gadolinium oxide; D012822:Silicon Dioxide; D005682:Gadolinium