title: Application of proteomics technology in adipocyte biology.
abstract: Obesity and its associated complications have reached epidemic proportions in Western-type societies. Concomitantly, the obesity incidence in developing countries is increasing. One hallmark of obesity is the differentiation of pre-adipocytes into mature triglyceride-loaded adipocytes present in subcutaneous and visceral adipose tissue depots. This may ultimately lead to dysfunctional adipose tissue together with detrimental changes in the profiles of (pre-)adipocyte-secreted proteins, known as adipokines. Obesity-induced alterations in adipokine profiles contribute to the development of obesity-associated disorders. Consequently, the interest in the molecular events responsible for adipose tissue modifications during weight gain and weight loss as well as in the aetiology of obesity-associated disorders is growing. Molecular mechanisms involved in pre-adipocyte differentiation and alterations in adipokine profiles have been examined at the gene and protein level by high-throughput technologies. Independent proteomics studies have contributed significantly to further insight into adipocyte biology, particularly with respect to adipokine profiling. In this review novel findings obtained with adipo-proteomics studies are highlighted and the relevance of proteomics technologies to further understand molecular aspects of adipocyte biology is discussed.
authors: Department of Human Biology, Maastricht University, P.O. Box 616, 6200 MD Maastricht, The Netherlands. j.renes@maastrichtuniversity.nl
authors: Johan
authors: J
authors: Renes
authors: Edwin
authors: E
authors: Mariman
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000502:physiology*; D050156:Adipogenesis; D054392:Adipokines / Q000378:metabolism; D000273:Adipose Tissue / Q000378:metabolism*; D006801:Humans; D009765:Obesity / Q000378:metabolism* / Q000503:physiopathology*; D011506:Proteins / Q000378:metabolism*; D040901:Proteomics
chemical_list: D054392:Adipokines; D011506:Proteins