title: Efficient harvesting of marine microalgae Nannochloropsis maritima using magnetic nanoparticles.
abstract: An efficient magnetic separation technology using Fe(3)O(4) nanoparticles was developed for harvesting marine microalgae Nannochloropsis maritima from culture broth. Recovery capacity of these nanoparticles was affected by microalgal growth phase and reached the peak value when the microalgal growth reached its maximal biomass after 18 days. The recovery efficiency of microalgal cells from the culture medium reached more than 95% at the particle dosage of 120 mg/L within 4 min. Electrostatic attraction at acidic pH and cell aggregation under neutral and alkaline conditions was beneficial for harvesting the algal cells. Higher operation temperature resulted in higher adsorption capacity of these nanoparticles for microalgawl cells. Reuse of the culture medium obtained from magnetic separation gave similar biomass production in comparison with that from centrifugation separation after 5 recycles. Together with these results provide a great potential in high-efficient and economical harvesting of tiny marine microalgae using magnetic separation technology in practice.
authors: National Key Laboratory of Biochemical Engineering & Laboratory of Separation Science and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.
authors: Yi-Ru
authors: YR
authors: Hu
authors: Feng
authors: F
authors: Wang
authors: Shi-Kai
authors: SK
authors: Wang
authors: Chun-Zhao
authors: CZ
authors: Liu
authors: Chen
authors: C
authors: Guo
mesh_terms: D000327:Adsorption; D003470:Culture Media / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D008280:Magnetics; D058086:Microalgae / Q000166:cytology / Q000187:drug effects / Q000254:growth & development* / Q000302:isolation & purification; D053758:Nanoparticles / Q000737:chemistry*; D059027:Recycling; D058009:Stramenopiles / Q000166:cytology / Q000187:drug effects / Q000254:growth & development* / Q000302:isolation & purification; D013696:Temperature; D013997:Time Factors
chemical_list: D003470:Culture Media