title: Antimalarial drug chloroquine counteracts activation of indoleamine (2,3)-dioxygenase activity in human PBMC.
abstract: Antimalarial chloroquine is also used for the treatment of immune-mediated diseases. The interference of chloroquine with interferon-γ-induced tryptophan breakdown and neopterin production has been investigated in human peripheral blood mononuclear cells (PBMC) in vitro. Micromolar concentrations (2-50 μM) of chloroquine dose-dependently suppressed mitogen-induced tryptophan breakdown in PBMC but not in the myelomonocytic THP-1-Blue cell line, after 48 h of treatment. In stimulated PBMC, neopterin production was super-induced by 10 μM chloroquine, while it was significantly suppressed at a concentration of 50 μM. These anti-inflammatory effects may relate to the therapeutic benefit of chloroquine in inflammatory conditions and may widen the spectrum of its clinical applications.
authors: Division of Medical Biochemistry, Biocenter, Innsbruck Medical University, 6020 Innsbruck, Austria.
authors: Johanna M
authors: JM
authors: Gostner
authors: Sebastian
authors: S
authors: Schröcksnadel
authors: Kathrin
authors: K
authors: Becker
authors: Marcel
authors: M
authors: Jenny
authors: Harald
authors: H
authors: Schennach
authors: Florian
authors: F
authors: Uberall
authors: Dietmar
authors: D
authors: Fuchs
keywords: Chloroquine; Indoleamine (2,3)-dioxygenase (IDO); Neopterin; PBMC; Tryptophan
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