title: Precise comparison of protein localization among OCT, OAT, and MATE in human kidney.
abstract: Organic anion transporters (OATs) and organic cation transporters (OCT) play pivotal roles in the uptake of drugs into epithelial cells at the basolateral membranes, and multidrug and toxin extrusion (MATE) mediates drug secretion into urine at the brush-border membranes. In this study, the expression and distribution of apical MATE1 and MATE2-K, and basolateral OAT1, OAT3, and OCT2 were compared using serial sections of human kidney cortex. First, mRNA expression in the proximal tubules was evaluated using laser microdissection. Levels of OAT, OCT2, and MATE mRNA in the proximal tubules were greatly higher compared with glomerulus. The results quantitatively indicated that these transporters were localized to proximal tubules in the renal cortex. Second, MATE1 and MATE2-K protein were detected in proximal epithelial cells in which OCT2 protein was expressed at the basolateral membranes. In addition, MATE1 was expressed at the brush-border membranes of tubular epithelial cells in which OAT1 and OAT3 were expressed. The results confirmed that OAT1, OAT3, OCT2, MATE1, and MATE2-K were coexpressed in tubular epithelial cells. The cooperation among OAT, OCT, and MATE in renal drug secretion was consistent with their distribution.
authors: Kyoto Pharmaceutical University, Yamashina-ku, Kyoto 607-8414, Japan.
authors: Hideyuki
authors: H
authors: Motohashi
authors: Yumi
authors: Y
authors: Nakao
authors: Satohiro
authors: S
authors: Masuda
authors: Toshiya
authors: T
authors: Katsura
authors: Tomomi
authors: T
authors: Kamba
authors: Osamu
authors: O
authors: Ogawa
authors: Ken-Ichi
authors: K
authors: Inui
mesh_terms: D000368:Aged; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D008875:Middle Aged; D027362:Organic Anion Transport Protein 1 / Q000032:analysis* / Q000235:genetics; D029362:Organic Anion Transporters, Sodium-Independent / Q000032:analysis* / Q000235:genetics; D027701:Organic Cation Transport Proteins / Q000032:analysis* / Q000235:genetics; D000075102:Organic Cation Transporter 2; D012333:RNA, Messenger / Q000032:analysis / Q000235:genetics
chemical_list: D027362:Organic Anion Transport Protein 1; D029362:Organic Anion Transporters, Sodium-Independent; D027701:Organic Cation Transport Proteins; D000075102:Organic Cation Transporter 2; D012333:RNA, Messenger; C491013:SLC22A2 protein, human; C521869:SLC47A1 protein, human; C521870:SLC47A2 protein, human; C114338:organic anion transport protein 3
keywords: active transport; drug transport; elimination; membrane transporter; multidrug and toxin extrusion; organic anion transporter; organic cation transporter; renal clearance; renal excretion