title: Twenty-one proteins up-regulated in human H-ras oncogene transgenic rat pancreas cancers are up-regulated in human pancreas cancer.
abstract: OBJECTIVE
  We have established rat models of pancreatic ductal adenocarcinoma (PDAC) in which expression of a human H-ras(G12V) or K-ras(G12V) oncogene regulated by the Cre/lox system drives pancreatic carcinogenesis. Pancreatic ductal adenocarcinoma which develops in H-ras(G12V) and K-ras(G12V) transgenic rats is cytogenetically and histopathologically similar to human PDAC. The present study was designed to determine the feasibility of using the commercially available H-ras(G12V) transgenic rat to find diagnostic protein biomarkers for human pancreatic cancer.
  
  
  METHODS
  For an animal model to be useful for searching for protein biomarkers for a disease, it is essential that proteins that are up-regulated in the model are also up-regulated in humans. We used liquid chromatography-tandem mass spectrometry (LC-MS/MS) to compare H-ras(G12V) transgenic rat PDAC with surrounding normal pancreas tissue.
  
  
  RESULTS
  We identified 30 up-regulated proteins in the H-ras(G12V) transgenic rat PDAC lesions; importantly, 21 human homologs of these 30 rat proteins are up-regulated in human pancreatic cancer patients.
  
  
  CONCLUSIONS
  These results indicate that numerous proteins that are up-regulated in H-ras(G12V) transgenic rat PDAC are also up-regulated in human pancreatic cancer; therefore, this rat model can be used to search for diagnostic biomarkers for this disease.
authors: Environmental Health Science Laboratory, Sumitomo Chemical Co., Ltd., Konohana-ku, Osaka, Japan. yabushitas@sc.sumitomo-chem.co.jp
authors: Setsuko
authors: S
authors: Yabushita
authors: Katsumi
authors: K
authors: Fukamachi
authors: Fumitake
authors: F
authors: Kikuchi
authors: Masakazu
authors: M
authors: Ozaki
authors: Kaori
authors: K
authors: Miyata
authors: Tokuo
authors: T
authors: Sukata
authors: Yoshihito
authors: Y
authors: Deguchi
authors: Hajime
authors: H
authors: Tanaka
authors: Anna
authors: A
authors: Kakehashi
authors: Satoshi
authors: S
authors: Kawamura
authors: Satoshi
authors: S
authors: Uwagawa
authors: Hideki
authors: H
authors: Wanibuchi
authors: Masumi
authors: M
authors: Suzui
authors: David B
authors: DB
authors: Alexander
authors: Hiroyuki
authors: H
authors: Tsuda
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D021441:Carcinoma, Pancreatic Ductal / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002853:Chromatography, Liquid; D004195:Disease Models, Animal; D005240:Feasibility Studies; D006801:Humans; D009154:Mutation; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D055647:Rats, Transgenic; D053719:Tandem Mass Spectrometry; D015854:Up-Regulation; D018631:ras Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D011506:Proteins; D018631:ras Proteins