title: Directed neuronal differentiation of mouse embryonic and induced pluripotent stem cells and their gene expression profiles.
abstract: Embryonic stem cells (ESCs) may be useful as a therapeutic source of cells for the production of healthy tissue; however, they are associated with certain challenges including immunorejection as well as ethical issues. Induced pluripotent stem cells (iPSCs) are a promising substitute since a patient's own adult cells would serve as tissue precursors. Ethical concerns prevent a full evaluation of the developmental potency of human ESCs and iPSCs, therefore, mouse iPSC models are required for protocol development and safety assessments. We used a modified culturing protocol to differentiate pluripotent cells from a mouse iPS cell line and two mouse ES cell lines into neurons. Our results indicated that all three pluripotent stem cell lines underwent nearly the same differentiation process when induced to form neurons in vitro. Genomic expression microarray profiling and single-cell RT-qPCR were used to analyze the neural lineage differentiation process, and more than one thousand differentially expressed genes involved in multiple molecular processes relevant to neural development were identified.
authors: Laboratory of Developmental Biology, Institute of Medical Science, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P.R. China.
authors: Xuesong
authors: X
authors: Chen
authors: Qi
authors: Q
authors: Gu
authors: Xiang
authors: X
authors: Wang
authors: Qingwen
authors: Q
authors: Ma
authors: Huixiang
authors: H
authors: Tang
authors: Xiaoshuang
authors: X
authors: Yan
authors: Xinbing
authors: X
authors: Guo
authors: Hao
authors: H
authors: Yan
authors: Jie
authors: J
authors: Hao
authors: Fanyi
authors: F
authors: Zeng
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D017077:Culture Media, Conditioned / Q000494:pharmacology; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D055495:Neurogenesis / Q000235:genetics; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D059467:Transcriptome
chemical_list: D017077:Culture Media, Conditioned