title: Barnacle cement as surface anchor for "clicking" of antifouling and antimicrobial polymer brushes on stainless steel.
abstract: Barnacle cement (BC) was utilized 'beneficially' as a surface anchor on stainless steel (SS) for coupling of functional polymer brushes via "click" reactions in both "grafting-to" and "grafting-from" processes. Ethylene sulfide (ES), propargyl carbonylimidazole (PPC) and azidoethyl carbonylimidazole (AEC) reacted with amine and/or hydroxyl groups in BC to introduce the corresponding thiol, alkyne, and azide groups on SS surfaces (SS-thiol, SS-alkyne, and SS-azide, respectively). Antifouling zwitterionic SS-PMPC surface was prepared by thiol-ene photopolymerization of 2-methacryloyloxyethyl phosphorylcholine (MPC) from the SS-thiol surface. Protein-resistant SS-PPEGMA and protein-adsorbing SS-PPFS surfaces were prepared by coupling of the respective azide-functionalized poly(poly(ethylene glycol)methyl ether methacrylate) (azido-PPEGMA) and poly(2,3,4,5,6-pentafluorostyrene) (azido-PPFS) polymer brushes in azide-alkyne "click" reaction. Antifouling alkyne-functionalized poly(N-hydroxyethyl acrylamide) (alkynyl-PHEAA) and antibacterial alkyne-functionalized poly(2-(methacryloyloxy)ethyl trimethylammonium chloride) (alkynyl-PMETA) polymer brushes were clicked on the SS-azide surface. Adsorption of bovine serum albumin and bacteria fouling of Gram-negative Escherichia coli ( E. coli ) and Gram-positive Staphylococcus epidermidis ( S. epidermidis ) were investigated on the polymer-functionalized SS surfaces. The versatile bioanchor and functional polymer brush coatings are stable in an abiotic aqueous environment for over a month.
authors: NUS Graduate School for Integrative Science and Engineering, National University of Singapore , Kent Ridge, Singapore 117576.
authors: Wen Jing
authors: WJ
authors: Yang
authors: Tao
authors: T
authors: Cai
authors: Koon-Gee
authors: KG
authors: Neoh
authors: En-Tang
authors: ET
authors: Kang
authors: Serena Lay-Ming
authors: SL
authors: Teo
authors: Daniel
authors: D
authors: Rittschof
mesh_terms: D000269:Adhesives; D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology*; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D011108:Polymers / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D013193:Stainless Steel; D001468:Thoracica / Q000737:chemistry*
chemical_list: D000269:Adhesives; D000890:Anti-Infective Agents; D011108:Polymers; D013193:Stainless Steel