title: Extended C-terminus and length of the linker connecting the G-domains are species-specific variations in the EngA family of GTPases.
abstract: EngA is an essential protein involved in ribosome biogenesis. It is an unique GTPase, possessing two consecutive G-domains. Using sequence and phylogenetic analysis, we found two intriguing variants among EngA homologues - one with a shorter linker joining the G-domains and another with a longer linker, which additionally possesses an extended C-terminus. Interestingly, while the former variant is mainly restricted to firmicutes, the latter is found in nonfirmicutes. Chimeric proteins with interchanged linkers and extensions were generated to gauge the importance of these elements. Ribosome interaction experiments employing the chimeric proteins suggest that a precise combination of the linker and C-terminal extension are important features regulating EngA ribosome interactions in a variant-specific manner.
authors: Department of Biological Sciences and Bioengineering, Indian Institute of Technology, Kanpur 208016, India.
authors: Sushil Kumar
authors: SK
authors: Tomar
authors: Prashant
authors: P
authors: Kumar
authors: Soneya
authors: S
authors: Majumdar
authors: Varun
authors: V
authors: Bhaskar
authors: Prasun
authors: P
authors: Dutta
authors: Balaji
authors: B
authors: Prakash
keywords: Chimeric proteins; Conserved bacterial GTPase; EngA; Ribosome biogenesis; Sequence analysis; Species-specific variations
references: Bioinformatics. 2006 Jul 1;22(13):1658-9
references: 16731699
references: Bioinformatics. 2007 Nov 1;23(21):2947-8
references: 17846036
references: Nucleic Acids Res. 2008 Jul 1;36(Web Server issue):W197-201
references: 18463136
references: J Bacteriol. 2006 Dec;188(23):8252-8
references: 16997968
references: Mol Biol Evol. 1987 Jul;4(4):406-25
references: 3447015
references: Mol Biol Evol. 2007 Aug;24(8):1596-9
references: 17488738
references: Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
references: 9254694
references: Structure. 2002 Dec;10(12):1649-58
references: 12467572
references: Proc Natl Acad Sci U S A. 2006 Aug 15;103(33):12359-64
references: 16894162
references: Mol Microbiol. 2006 Sep;61(6):1660-72
references: 16930151
references: Nucleic Acids Res. 2006 Apr 28;34(8):2196-205
references: 16648363
references: Microbiol Mol Biol Rev. 2007 Sep;71(3):477-94
references: 17804668
references: Annu Rev Microbiol. 2009;63:155-76
references: 19575570
references: J Bacteriol. 2006 Nov;188(22):7992-6
references: 16963571
references: Bioinformatics. 2009 May 1;25(9):1189-91
references: 19151095
references: Nucleic Acids Res. 2009 Apr;37(7):2359-70
references: 19246542
references: Biochem Biophys Res Commun. 2009 Sep 4;386(4):602-6
references: 19540197
references: Biochem Biophys Res Commun. 2009 Feb 6;379(2):201-5
references: 19109926
references: Evolution. 1985 Jul;39(4):783-791
references: 28561359