title: Natural killer cell-mediated contact sensitivity develops rapidly and depends on interferon-α, interferon-γ and interleukin-12.
abstract: Natural killer (NK) cell-mediated contact sensitivity was recently described in mice. Here, we confirm NK cell-mediated contact sensitivity (CS) in SCID and RAG1(-/-) mice but not in SCIDbeige mice, which have non-functional NK cells that lack NK cell granules. NK cell-mediated CS was transferred by liver mononuclear cells and the DX5(+) fraction of liver cells, confirming that NK cells mediate CS in the absence of T and B cells. Participation of NKT cells and B-1 cells was ruled out using Jα18(-/-) and JH(-/-) mice, respectively. Remarkably, NK cell-mediated CS was observed just 1 hr after immunization and was detectable as early as 30 min after challenge. Further, we examined cytokine requirements for NK cell-mediated CS, and found that liver mononuclear cells from interleukin-12(-/-) , interferon-γ(-/-) and interferon-α receptor(-/-) donors fail to transfer NK cell-mediated CS to naive hosts. Our studies clearly show that dinitrofluorobenzene sensitized NK cells mediate very rapid, antigen-specific cell-mediated immunity, with features of both innate and acquired immune responses.
authors: Department of Medical Biology, Jagiellonian University College of Medicine, Kraków, Poland.
authors: Monika
authors: M
authors: Majewska-Szczepanik
authors: Silke
authors: S
authors: Paust
authors: Ulrich H
authors: UH
authors: von Andrian
authors: Philip W
authors: PW
authors: Askenase
authors: Marian
authors: M
authors: Szczepanik
mesh_terms: D056704:Adaptive Immunity; D019264:Adoptive Transfer; D000818:Animals; D003877:Dermatitis, Contact / Q000209:etiology* / Q000276:immunology*; D005260:Female; D007113:Immunity, Innate; D016898:Interferon-alpha / Q000378:metabolism*; D007371:Interferon-gamma / Q000378:metabolism*; D018664:Interleukin-12 / Q000378:metabolism*; D007694:Killer Cells, Natural / Q000276:immunology* / Q000473:pathology; D008099:Liver / Q000276:immunology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D018345:Mice, Knockout; D016513:Mice, SCID; D054387:Receptors, CXCR / Q000378:metabolism; D000075744:Receptors, CXCR6; D013997:Time Factors
chemical_list: C482906:Cxcr6 protein, mouse; D016898:Interferon-alpha; D054387:Receptors, CXCR; D000075744:Receptors, CXCR6; D018664:Interleukin-12; D007371:Interferon-gamma
keywords: contact sensitivity; cytokines; liver mononuclear cells; natural killer cells
references: J Exp Med. 2012 May 7;209(5):947-54
references: 22493516
references: J Immunol. 1999 Aug 15;163(4):1943-50
references: 10438930
references: J Exp Med. 2007 Oct 29;204(11):2561-8
references: 17923504
references: Scand J Immunol. 2011 May;73(5):465-77
references: 21272050
references: Clin Exp Immunol. 2001 Sep;125(3):345-50
references: 11531940
references: Nat Immunol. 2006 May;7(5):507-16
references: 16617337
references: Nat Immunol. 2010 Dec;11(12):1127-35
references: 20972432
references: J Immunol. 2002 Mar 15;168(6):3079-87
references: 11884482
references: Immunol Cell Biol. 2012 Sep;90(8):784-95
references: 22290507
references: Immunol Res. 2011 Aug;50(2-3):248-54
references: 21717064
references: Lab Anim Sci. 1993 Aug;43(4):291-5
references: 8231084
references: J Immunol. 2008 Aug 1;181(3):1717-27
references: 18641308
references: Immunol Rev. 2010 May;235(1):297-305
references: 20536571
references: J Exp Med. 2007 Oct 29;204(11):2569-78
references: 17923507
references: Trends Immunol. 2004 Aug;25(8):441-9
references: 15275644
references: Int Immunopharmacol. 2002 Feb;2(2-3):201-11
references: 11811925
references: J Immunol. 2009 Jul 15;183(2):1463-70
references: 19553527
references: J Exp Med. 2003 Dec 15;198(12):1785-96
references: 14676294
references: Eur J Immunol. 1995 Nov;25(11):3006-10
references: 7489735
references: J Immunol. 2009 Sep 1;183(5):2911-4
references: 19648279
references: Eur J Immunol. 2012 Jan;42(1):80-8
references: 21968602
references: J Immunol. 2005 Dec 1;175(11):7170-8
references: 16301620
references: Immunity. 1995 Mar;2(3):223-38
references: 7697543
references: J Exp Med. 1996 Dec 1;184(6):2129-39
references: 8976169
references: J Exp Med. 2002 Nov 18;196(10):1277-90
references: 12438420
references: J Immunol. 1995 Apr 15;154(8):3644-53
references: 7706708
references: Br J Dermatol. 2009 Dec;161(6):1301-6
references: 19785613
references: Trends Immunol. 2007 Jun;28(6):252-9
references: 17466596
references: J Immunol. 1996 Feb 1;156(3):1138-42
references: 8557990
references: Contact Dermatitis. 2002 Jan;46(1):1-5
references: 11918579
references: PLoS Pathog. 2011 Aug;7(8):e1002141
references: 21829360