title: [Method of selective demonstration of proliferating cells in the structures of developing nervous system based on the detection of phosphorylated H3 histone].
abstract: Phosphorylated H3 histone is of interest for the study of proliferative activity of the cells in various tissues. Its detection is used in histopathological tumor diagnosis. The purpose of this study was to develop immunocytochemical protocols for the demonstration of H3 phosphohistone in the cells of the developing nervous system for classical light microscopy and confocal laser microscopy. The protocols presented allow to identify the dividing cells in the structures of developing brain with high selectivity and good reproducibility. Zinc-ethanol-formaldehyde is recommended as an optimal fixative for H3 phosphohistone.
authors: RAMS North-Western Branch Institute of Experimental Medicine, St. Petersburg, Russia
authors: D E
authors: DE
authors: Korzhevskiy
authors: O V
authors: OV
authors: Kirik
authors: G V
authors: GV
authors: Beznin
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology* / Q000254:growth & development; D049109:Cell Proliferation; D005404:Fixatives; D006657:Histones / Q000032:analysis* / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D009474:Neurons / Q000737:chemistry* / Q000502:physiology; D010766:Phosphorylation; D051381:Rats; D015203:Reproducibility of Results
chemical_list: D005404:Fixatives; D006657:Histones