title: Dysregulation of miR-31 and miR-375 expression is associated with clinical outcomes in oral carcinoma.
abstract: OBJECTIVE
  To identify differentially expressed miRNA between oral squamous cell carcinoma (OSCC) and non-cancer (NC) and to associate these with clinico-pathological parameters.
  
  
  METHODS
  miRNA microarray profiling was utilized to obtain the expression profile of miRNAs in four OSCC and four NC samples. The expression of miR-31 and miR-375 was further validated in 26 OSCC and three NC samples using real-time-PCR. The association between miRNA expression and clinico-pathological parameters was tested by univariate and multivariate analyses.
  
  
  RESULTS
  Microarray profiling demonstrated that 15 and four miRNAs were up-regulated and down-regulated, respectively, in OSCC as compared with NC. miR-31 and miR-375 were validated as up- and down-regulated miRNAs, respectively. In univariate analyses, expression of miR-31 was significantly elevated in early stage, tumours with no metastatic nodes and those from the buccal mucosa. By contrast, low miR-375 expression was significantly associated with late stage disease, larger tumour size and the non-cohesive type of pattern of invasion in OSCC. The association between miR-31 expression with tumour staging and site and miR-375 with tumour staging remained significant in multivariate analyses.
  
  
  CONCLUSIONS
  This study has identified 19 miRNAs significantly associated with OSCC, and expressions of miR-31 and miR-375 were significantly related with clinico-pathological parameters suggesting they could be important in driving oral tumourigenesis.
authors: Faculty of Dentistry, Oral Cancer Research & Coordinating Center (OCRCC), University of Malaya, Kuala Lumpur, Malaysia; Department of Oral Pathology, Oral Medicine & Periodontology, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia.
authors: M Y
authors: MY
authors: Siow
authors: L P Karen
authors: LP
authors: Ng
authors: V K
authors: VK
authors: Vincent-Chong
authors: M
authors: M
authors: Jamaludin
authors: M T
authors: MT
authors: Abraham
authors: Z A
authors: ZA
authors: Abdul Rahman
authors: T G
authors: TG
authors: Kallarakkal
authors: Y-H
authors: YH
authors: Yang
authors: S C
authors: SC
authors: Cheong
authors: R B
authors: RB
authors: Zain
mesh_terms: D000328:Adult; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: C534612:MIRN31 microRNA, human; C540933:MIRN375 microRNA, human; D035683:MicroRNAs
keywords: clinico-pathological parameters; miR-31; miR-375; microRNA profiling; oral squamous cell carcinoma