title: Atomic hydrogen surrounded by water molecules, H(H2O)m, modulates basal and UV-induced gene expressions in human skin in vivo.
abstract: Recently, there has been much effort to find effective ingredients which can prevent or retard cutaneous skin aging after topical or systemic use. Here, we investigated the effects of the atomic hydrogen surrounded by water molecules, H(H2O)m, on acute UV-induced responses and as well as skin aging. Interestingly, we observed that H(H2O)m application to human skin prevented UV-induced erythema and DNA damage. And H(H2O)m significantly prevented UV-induced MMP-1, COX-2, IL-6 and IL-1β mRNA expressions in human skin in vivo. We found that H(H2O)m prevented UV-induced ROS generation and inhibited UV-induced MMP-1, COX-2 and IL-6 expressions, and UV-induced JNK and c-Jun phosphorylation in HaCaT cells. Next, we investigated the effects of H(H2O)m on intrinsically aged or photoaged skin of elderly subjects. In intrinsically aged skin, H(H2O)m application significantly reduced constitutive expressions of MMP-1, IL-6, and IL-1β mRNA. Additionally, H(H2O)m significantly increased procollagen mRNA and also decreased MMP-1 and IL-6 mRNA expressions in photoaged facial skin. These results demonstrated that local application of H(H2O)m may prevent UV-induced skin inflammation and can modulate intrinsic skin aging and photoaging processes. Therefore, we suggest that modifying the atmospheric gas environment within a room may be a new way to regulate skin functions or skin aging.
authors: Department of Dermatology, Seoul National University College of Medicine, Seoul, Korea.
authors: Mi Hee
authors: MH
authors: Shin
authors: Raeeun
authors: R
authors: Park
authors: Hideo
authors: H
authors: Nojima
authors: Hyung-Chel
authors: HC
authors: Kim
authors: Yeon Kyung
authors: YK
authors: Kim
authors: Jin Ho
authors: JH
authors: Chung
mesh_terms: D000367:Age Factors; D002461:Cell Line, Transformed; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D004781:Environmental Exposure; D004789:Enzyme Activation / Q000187:drug effects / Q000528:radiation effects; D004890:Erythema / Q000209:etiology / Q000517:prevention & control; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000528:radiation effects*; D006801:Humans; D006859:Hydrogen / Q000494:pharmacology*; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D048670:MAP Kinase Kinase 4 / Q000378:metabolism; D020781:Matrix Metalloproteinase 1 / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects / Q000528:radiation effects; D011347:Procollagen / Q000235:genetics / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D012867:Skin / Q000378:metabolism*; D015595:Skin Aging / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D013936:Thymidine / Q000737:chemistry / Q000528:radiation effects; D014466:Ultraviolet Rays / Q000009:adverse effects*; D014867:Water / Q000737:chemistry
chemical_list: D053583:Interleukin-1beta; D015850:Interleukin-6; D011347:Procollagen; D016755:Proto-Oncogene Proteins c-jun; D017382:Reactive Oxygen Species; D014867:Water; D006859:Hydrogen; D051546:Cyclooxygenase 2; D048670:MAP Kinase Kinase 4; D020781:Matrix Metalloproteinase 1; D013936:Thymidine
references: J Clin Invest. 1998 Mar 15;101(6):1432-40
references: 9502786
references: Photochem Photobiol. 1997 Feb;65(2):355-65
references: 9066312
references: Int J Oncol. 2005 Jan;26(1):169-76
references: 15586237
references: Biochem Biophys Res Commun. 2000 Mar 5;269(1):131-6
references: 10694489
references: Eur J Appl Physiol. 2000 Mar;81(4):325-8
references: 10664092
references: J Lipid Res. 2005 Aug;46(8):1712-20
references: 15930517
references: J Invest Dermatol. 1989 Feb;92(2):235-9
references: 2783951
references: Methods. 2001 Dec;25(4):402-8
references: 11846609
references: Nature. 1996 Oct 3;383(6599):453-7
references: 8837781
references: Ann N Y Acad Sci. 2002 Nov;973:31-43
references: 12485830
references: Cancer Lett. 2006 May 8;236(1):13-23
references: 15978720
references: J Invest Dermatol. 2000 Mar;114(3):480-6
references: 10692106
references: Photodermatol Photoimmunol Photomed. 2000 Dec;16(6):239-44
references: 11132125
references: J Phys D Appl Phys. 2007 Jan 21;40(2):501-509
references: 32287388
references: Exp Gerontol. 2000 Aug;35(5):521-32
references: 10978675
references: Curr Probl Dermatol. 2001;29:83-94
references: 11225204
references: Arch Dermatol Res. 2006 Apr;297(10):477-9
references: 16491351
references: Exp Dermatol. 2011 Sep;20(9):749-55
references: 21707755
references: J Biol Chem. 2005 Mar 4;280(9):8079-85
references: 15579469
references: Chin Med J (Engl). 2006 May 20;119(10):827-31
references: 16732985
references: Ann N Y Acad Sci. 1994 Apr 18;714:136-42
references: 8017762
references: Int J Mol Med. 2009 Apr;23(4):555-60
references: 19288033
references: J Invest Dermatol. 2009 Feb;129(2):460-7
references: 18719610
references: J Invest Dermatol. 1991 Apr;96(4):484-9
references: 2007786
references: Photochem Photobiol. 2008 Mar-Apr;84(2):322-9
references: 18194346
references: Arch Dermatol Res. 1996;288(1):39-44
references: 8750933
references: Exp Gerontol. 2010 Jun;45(6):419-26
references: 20211236
references: Clin Cancer Res. 2000 Oct;6(10):3864-9
references: 11051231
references: J Investig Dermatol Symp Proc. 2009 Aug;14(1):20-4
references: 19675548
references: Nature. 1996 Jan 25;379(6563):335-9
references: 8552187
references: Br J Pharmacol. 1995 Mar;114(6):1257-65
references: 7620717
references: Am J Pathol. 2009 Jan;174(1):101-14
references: 19116368
references: Acta Neurochir Suppl. 2011;111:237-41
references: 21725762
references: Curr Cancer Drug Targets. 2007 Jun;7(4):325-34
references: 17979627
references: Mech Ageing Dev. 2003 Aug-Sep;124(8-9):903-10
references: 14499495
references: J Invest Dermatol. 2003 May;120(5):835-41
references: 12713590
references: J Invest Dermatol. 2000 Aug;115(2):177-82
references: 10951233
references: Photochem Photobiol. 1994 May;59(5):550-6
references: 8041811
references: Biochem Biophys Res Commun. 1995 Jan 17;206(2):474-9
references: 7826364
references: Free Radic Res. 2010 Sep;44(9):971-82
references: 20815764
references: J Hazard Mater. 2006 Dec 1;138(3):518-25
references: 16846682
references: Toxicology. 2003 Jul 15;189(1-2):21-39
references: 12821280
references: Mech Ageing Dev. 2002 Nov;123(12):1589-95
references: 12470896
references: J Eur Acad Dermatol Venereol. 2003 Nov;17(6):663-9
references: 14761133
references: Transplantation. 2010 Dec 27;90(12):1344-51
references: 21048533
references: Ageing Res Rev. 2002 Sep;1(4):705-20
references: 12208239
references: J Investig Dermatol Symp Proc. 1998 Aug;3(1):61-8
references: 9732061
references: J Biol Chem. 1998 Jul 24;273(30):18891-7
references: 9668065
references: Biol Chem. 1997 Nov;378(11):1247-57
references: 9426184
references: J Invest Dermatol. 2010 Jun;130(6):1697-706
references: 20164845
references: N Engl J Med. 1997 Nov 13;337(20):1419-28
references: 9358139
references: J Invest Dermatol. 2005 Aug;125(2):221-9
references: 16098030
references: Nat Med. 2007 Jun;13(6):688-94
references: 17486089
references: Int J Cancer. 1998 Apr 13;76(2):201-6
references: 9537581
references: Shock. 2010 Nov;34(5):495-501
references: 20351628
references: Am J Ind Med. 2009 Aug;52(8):654-61
references: 19507184