title: Efficacy of anti-staphylococcal protein P128 for the treatment of canine pyoderma: potential applications.
abstract: In this study, we demonstrate the antibacterial activity of P128 on Staphylococcus isolates responsible for canine pyoderma. Eighty seven swabs were collected from dogs suffering from pyoderma and subjected to antibiotic sensitivity test and 46 Staphylococcus strains were isolated and characterized. In-vitro antimicrobial susceptibility testing with P128 was done by Minimum Inhibitory Concentration (MIC) method as per CLSI guidelines. All the Staphylococci isolated from the dogs with pyoderma, although showed resistance to various antibiotics tested, were lysed by P128. Clinical efficacy of P128 was examined in 17 dogs with pyoderma by application of the P128 hydrogel twice daily for 8 days and the results indicated complete healing of all the lesions of all the dogs under treatment. Under the conditions of this study, P128 was found to be a potent convenient proteinaceous drug for the treatment of staphylococcal pyoderma in dogs.
authors: Gangagen Biotechnologies Pvt. Ltd., Yeshwantpur, Bangalore, 560022, India.
authors: Raghu Patil
authors: RP
authors: Junjappa
authors: Srividya Narayanamurthy
authors: SN
authors: Desai
authors: Panchali
authors: P
authors: Roy
authors: Nagalakshmi
authors: N
authors: Narasimhaswamy
authors: Juliet Roshini Mohan
authors: JR
authors: Raj
authors: Murali
authors: M
authors: Durgaiah
authors: Aradhana
authors: A
authors: Vipra
authors: Udaya Ravi
authors: UR
authors: Bhat
authors: Smitha Komarla
authors: SK
authors: Satyanarayana
authors: Nandini
authors: N
authors: Shankara
authors: Suneelkumar Muragesh
authors: SM
authors: Basingi
authors: Jagadeesh Janardhan
authors: JJ
authors: Bhat
authors: Sukumar
authors: S
authors: Hariharan
authors: Bharathi
authors: B
authors: Sriram
authors: Sriram
authors: S
authors: Padmanabhan
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000188:drug therapy* / Q000382:microbiology; D004285:Dogs; D005260:Female; D008297:Male; D008826:Microbial Sensitivity Tests / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length; D011711:Pyoderma / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary*; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000627:therapeutic use*; D013210:Staphylococcus / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification
chemical_list: C577473:P128 antistaphylococcal chimeric protein; D011993:Recombinant Fusion Proteins
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