title: Simultaneous evaluation of p53 and p21 expression level for early cancer diagnosis using SERS technique.
abstract: Simultaneous detection of tumor suppressor p53 and cyclin-dependent kinase inhibitor p21 has been achieved for the first time with a high sensitivity using surface enhanced Raman scattering (SERS) technique. In this immunoassay, gold@silver core-shell nanorods (Au@Ag NRs) with a high SERS activity and chemical stability were chosen to fabricate immuno-probes, which were conjugated with different antibodies to specifically target each analyte. Qualitative and quantitative analysis of p53 and p21 were achieved by characterizing SERS signals of two different Raman reporters (4MBA and DTNB), which were labeled on the corresponding optical probes. In addition, we investigated the detection of these biomarkers in the medium of blood serum. Our experimental results indicate the excellent specificity, high sensitivity (1 pg mL(-1)), as well as the great reproducibility of this immunoassay. More importantly, this assay presents a powerful ability to detect multiple analytes due to the great multiplexing capacity of SERS spectroscopy. Since the combined evaluation of p53 and p21 expression level acts as an indicator for early cancer prediction, our demonstrated immunoassay will potentially promote the application of SERS technique to practical clinical diagnoses.
authors: Advanced Photonics Centre, School of Electronic Science and Engineering, Southeast University, 2 Sipailou, Nanjing, Jiangsu, China. cyp@seu.edu.cn.
authors: Lei
authors: L
authors: Wu
authors: Zhuyuan
authors: Z
authors: Wang
authors: Shenfei
authors: S
authors: Zong
authors: Hui
authors: H
authors: Chen
authors: Chunlei
authors: C
authors: Wang
authors: Shuhong
authors: S
authors: Xu
authors: Yiping
authors: Y
authors: Cui
mesh_terms: D000818:Animals; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000378:metabolism*; D055088:Early Detection of Cancer / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D006046:Gold / Q000737:chemistry; D007118:Immunoassay; D043942:Nanotubes / Q000737:chemistry; D012834:Silver / Q000737:chemistry; D013059:Spectrum Analysis, Raman; D013499:Surface Properties; D013997:Time Factors; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D050759:Cyclin-Dependent Kinase Inhibitor p21; D016159:Tumor Suppressor Protein p53; D012834:Silver; D006046:Gold