title: Crocin enhanced functional recovery after sciatic nerve crush injury in rats.
abstract: OBJECTIVE
  Crocin is a constituent of saffron and has many biological functions. The present study aimed to investigate the effects of intraperitoneal (IP) injection of crocin on sciatic nerve regeneration in male Wistar rats.
  
  
  METHODS
  Fifty-four rats were divided into 9 groups: groups 1-4 (intact + normal saline and intact + crocin at doses of 5, 20 and 80 mg/kg, respectively); group 5 (sham surgery + normal saline); groups 6-9 (crush + normal saline and crush + crocin at doses of 5, 20 and 80 mg/kg, respectively). Normal saline and crocin were IP injected for 10 consecutive days after induction of a standard crush injury in left sciatic nerve. Footprints were obtained 1 day before and weekly after induction of nerve injury for evaluation of sciatic functional index (SFI). Blood samples were taken for evaluation of malondialdehyde (MDA) levels. Histopathological changes of sciatic nerve were investigated by light microscopy.
  
  
  RESULTS
  Sciatic nerve crush-injured rats showed SFI values reduction, increased plasma MDA levels and produced Wallerian degeneration in sciatic nerve. Crocin at a dose of 5 mg/kg had no significant effects. At doses of 20 and 80 mg/kg, crocin accelerated the SFI recovery, decreased MDA levels and reduced Wallerian degeneration severity.
  
  
  CONCLUSIONS
  The present study suggests that the neuroprotective effects afforded by crocin may be due in part to reduction of free radicals-induced toxic effects.
authors: Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
authors: Esmaeal
authors: E
authors: Tamaddonfard
authors: Amir Abbas
authors: AA
authors: Farshid
authors: Elham
authors: E
authors: Ahmadian
authors: Abbas
authors: A
authors: Hamidhoseyni
keywords: Crocin; Crush injury; Functional recovery; Rats; Sciatic nerve
references: J Bone Joint Surg Am. 1999 Nov;81(11):1600-10
references: 10565653
references: Phytother Res. 2010 Oct;24(10):1463-7
references: 20878695
references: Phytomedicine. 2010 Mar;17(3-4):227-32
references: 19747807
references: Pathol Res Pract. 2011 Sep 15;207(9):577-82
references: 21820814
references: Injury. 2012 Mar;43(3):284-9
references: 21684542
references: Phytother Res. 2010 Mar;24(3):410-3
references: 19653196
references: J Immunol. 2011 Nov 1;187(9):4788-99
references: 21964030
references: Biol Pharm Bull. 2005 Nov;28(11):2106-10
references: 16272698
references: J Neurosci Methods. 2010 Aug 30;191(2):277-82
references: 20637233
references: J Agric Food Chem. 2009 Sep 23;57(18):8325-30
references: 19754168
references: Brain Res. 2007 Mar 23;1138:86-94
references: 17274961
references: Muscle Nerve. 2009 Oct;40(4):582-8
references: 19645061
references: Brain Res. 2009 Jan 9;1247:11-20
references: 18983835
references: Appl Biochem Biotechnol. 2010 Sep;162(2):358-72
references: 19672721
references: J Neuroinflammation. 2011 Aug 30;8:109
references: 21878125
references: Plast Reconstr Surg. 1989 Jan;83(1):129-38
references: 2909054
references: Exp Oncol. 2007 Sep;29(3):175-80
references: 18004240
references: Fitoterapia. 2008 Apr;79(3):161-7
references: 18201841
references: Brain Pathol. 1999 Apr;9(2):313-25
references: 10219748
references: J Anat. 1999 Jan;194 ( Pt 1):1-14
references: 10227662
references: Microsurgery. 2002;22(6):234-41
references: 12375289
references: Avicenna J Phytomed. 2012 Spring;2(2):97-104
references: 25050237
references: Anal Biochem. 1979 Jun;95(2):351-8
references: 36810
references: Neurosurgery. 2002 Jul;51(1):212-9; discussion 219-20
references: 12182420
references: J Neurosci Res. 2012 Apr;90(4):842-8
references: 22253198
references: J Brachial Plex Peripher Nerve Inj. 2009 Nov 25;4:22
references: 19939272
references: J Brachial Plex Peripher Nerve Inj. 2006 Sep 29;1:2
references: 17147773
references: J Neurosci Methods. 2008 Aug 30;173(2):249-58
references: 18644327
references: Free Radic Res. 1998 Jun;28(6):659-71
references: 9736317
references: Phytomedicine. 2011 Apr 15;18(6):499-504
references: 21036580
references: J Med Food. 2006 Summer;9(2):246-53
references: 16822211
references: Iran J Basic Med Sci. 2013 Jan;16(1):101-7
references: 23638298
references: Brain Res. 2009 Sep 1;1287:58-66
references: 19555676
references: Neurosci Lett. 2010 Jul 5;478(2):66-71
references: 20438804
references: J Reconstr Microsurg. 2001 Jan;17(1):69-75
references: 11316287
references: Exp Neurol. 1994 Jun;127(2):284-90
references: 8033968
references: Microsurgery. 2007;27(8):673-7
references: 17941104
references: Eur J Pharmacol. 2011 Sep 30;667(1-3):222-9
references: 21616066
references: Eur J Pharmacol. 2010 Dec 1;648(1-3):110-6
references: 20854811
references: Pharmacol Rep. 2012;64(1):94-101
references: 22580525
references: Injury. 2005 Nov;36 Suppl 4:S24-9
references: 16288757