title: A tunable synthetic hydrogel system for culture of retinal ganglion cells and amacrine cells.
abstract: The central nervous system consists of complex groups of individual cells that receive electrical, chemical and physical signals from their local environment. Standard in vitro cell culture methods rely on two-dimensional (2-D) substrates that poorly simulate in vivo neural architecture. Neural cells grown in three-dimensional (3-D) culture systems may provide an opportunity to study more accurate representations of the in vivo environment than 2-D cultures. Furthermore, each specific type of neuron depends on discrete compositions and physical properties of their local environment. Previously, we developed a library of hydrogels composed of poly(ethylene glycol) and poly(l-lysine) which exhibit a wide range of mechanical properties. Here, we identified specific scaffolds from this library that readily support the survival, migration and neurite outgrowth of purified retinal ganglion cells and amacrine cells. These data address important biological questions about the interaction of neurons with the physical and chemical properties of their local environment and provide further insight for engineering neural tissue for cell-replacement therapies following injury.
authors: Bascom Palmer Eye Institute, Interdisciplinary Stem Cell Institute, University of Miami Miller School of Medicine, Miami, FL 33136, USA.
authors: Jonathan
authors: J
authors: Hertz
authors: Rebecca
authors: R
authors: Robinson
authors: Daniel A
authors: DA
authors: Valenzuela
authors: Erin B
authors: EB
authors: Lavik
authors: Jeffrey L
authors: JL
authors: Goldberg
mesh_terms: D025042:Amacrine Cells / Q000166:cytology* / Q000502:physiology*; D000818:Animals; D061249:Batch Cell Culture Techniques / Q000379:methods; D002415:Cats; D002478:Cells, Cultured; D018920:Coculture Techniques; D020100:Hydrogels / Q000737:chemistry*; D008422:Materials Testing; D011092:Polyethylene Glycols / Q000737:chemistry; D011107:Polylysine / Q000737:chemistry; D012165:Retinal Ganglion Cells / Q000166:cytology* / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D020100:Hydrogels; D011107:Polylysine; D011092:Polyethylene Glycols
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