title: Thiol-acrylate nanocomposite foams for critical size bone defect repair: A novel biomaterial.
abstract: Bone tissue engineering approaches using polymer/ceramic composites show promise as effective biocompatible, absorbable, and osteoinductive materials. A novel class of in situ polymerizing thiol-acrylate based copolymers synthesized via an amine-catalyzed Michael addition was studied for its potential to be used in bone defect repair. Both pentaerythritol triacrylate-co-trimethylolpropane tris(3-mercaptopropionate) (PETA-co-TMPTMP) and PETA-co-TMPTMP with hydroxyapatite (HA) composites were fabricated in solid cast and foamed forms. These materials were characterized chemically and mechanically followed by an in vitro evaluation of the biocompatibility and chemical stability in conjunction with human adipose-derived mesenchymal pluripotent stem cells (hASC). The solid PETA-co-TMPTMP with and without HA exhibited compressive strength in the range of 7-20 MPa, while the cytotoxicity and biocompatibility results demonstrate higher metabolic activity of hASC on PETA-co-TMPTMP than on a polycaprolactone control. Scanning electron microscope imaging of hASC show expected spindle shaped morphology when adhered to copolymer. Micro-CT analysis indicates open cell interconnected pores. Foamed PETA-co-TMPTMP HA composite shows promise as an alternative to FDA-approved biopolymers for bone tissue engineering applications.
authors: Department of Chemistry, Louisiana State University, 232 Choppin Hall, Louisiana 70803.
authors: Leah
authors: L
authors: Garber
authors: Cong
authors: C
authors: Chen
authors: Kameron V
authors: KV
authors: Kilchrist
authors: Christopher
authors: C
authors: Bounds
authors: John A
authors: JA
authors: Pojman
authors: Daniel
authors: D
authors: Hayes
mesh_terms: D000179:Acrylates / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D000273:Adipose Tissue / Q000166:cytology; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D001842:Bone and Bones / Q000187:drug effects / Q000473:pathology*; D016923:Cell Death / Q000187:drug effects; D019245:Compressive Strength / Q000187:drug effects; D004247:DNA / Q000378:metabolism; D006801:Humans; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites / Q000737:chemistry* / Q000633:toxicity; D058105:Polymerization; D011409:Propylene Glycols / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D054457:Tissue Scaffolds / Q000737:chemistry; D014945:Wound Healing / Q000187:drug effects*; D055114:X-Ray Microtomography
chemical_list: D000179:Acrylates; D001672:Biocompatible Materials; D011409:Propylene Glycols; C027993:trimethylolpropane triacrylate; D004247:DNA; C027992:pentaerythrityl triacrylate
keywords: PETA; adipose tissue; bone regeneration; scaffold; stem cells