title: Streptozotocin diabetes and insulin resistance impairment of spermatogenesis in adult rat testis: central vs. local mechanism.
abstract: Mammalian reproduction is dynamically regulated by the pituitary gonadotropins, luteinizing hormone (LH) and follicle-stimulating hormone (FSH). These hormones are synthesized in the pituitary gland following stimulation by the gonadotropin-releasing hormone (GnRH) and act by stimulating steroid production and gametogenesis in both males and females. Male adult Sprague-Dawley rats (120 - 140 g) were randomly divided into 7 groups. Group 1 &gt; Control group; fed on normal rat pellets. Group 2 &gt; Streptozotocin group; received a single dose IP injection of streptozotocin 45 mg/kg BW in Na+ citrate buffer pH 4.5. Group 3 &gt; Streptozotocin-insulin treated group; received a single dose IP injection of streptozotocin as in group 2 above and treated with insulin sub-cutaneously. Group 4 &gt; Streptozotocin-ginger treated group; received a single dose IP injection of streptozotocin as in group 2 above and treated with 500 mg/Kg Ginger extract orally. Group 5 &gt; Insulin resistant group; fed ad libitum on a special diet containing 25% fructose mixed with 75% normal rat chow (w/w). Group 6 &gt; Insulin resistant-pioglitazone treated group; fed ad libitum on a special diet as in group 5 above and treated with Pioglitazone 15 mg/kg orally. Group 7 &gt; Insulin resistant-ginger treated group; fed ad libitum on a special diet as in group 4 above, and also treated with 500 mg/Kg Ginger extract orally. Hormonal and tissue biochemistry analyses revealed that both central and local mechanisms are implicated in the impairment of spermatogenesis by diabetes but the hypothalamo-pituitary testicular axis alteration might not likely have a major impact as the local defect on steroidogenesis in the testis. This local defect could also predispose to male hypogonadism, i.e. failure of gonadal function.
authors: Department of Physiology, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, Idi-Araba, Lagos. arikawepaul2002@yahoo.co.uk
authors: A P
authors: AP
authors: Arikawe
authors: A
authors: A
authors: Oyerinde
authors: I I
authors: II
authors: Olatunji-Bello
authors: L F O
authors: LF
authors: Obika
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000150:complications* / Q000473:pathology; D020939:Zingiber officinale; D007248:Infertility, Male / Q000097:blood / Q000209:etiology*; D007328:Insulin; D007333:Insulin Resistance; D008297:Male; D010179:Pancreas / Q000473:pathology; D000077205:Pioglitazone; D010936:Plant Extracts; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013091:Spermatogenesis; D013311:Streptozocin; D013737:Testis / Q000378:metabolism*; D045162:Thiazolidinediones
chemical_list: D007328:Insulin; D010936:Plant Extracts; D045162:Thiazolidinediones; D013311:Streptozocin; D000077205:Pioglitazone