title: High prevalence of IgA class anti-neutrophil cytoplasmic antibodies (ANCA) is associated with increased risk of bacterial infection in patients with cirrhosis.
abstract: OBJECTIVE
  Anti-neutrophil cytoplasmic antibodies (ANCA) are a non-uniform family of antibodies recognizing diverse components of neutrophil granulocytes. ANCA formation might be induced by protracted bacterial infections or probably reflect an abnormal immune response to commensal microorganisms. Bacterial infections are common complications in cirrhosis with high incidence of episodes caused by enteric organisms, therefore, we sought to study the presence and clinical importance of ANCA in cirrhosis.
  
  
  METHODS
  Sera of 385 patients with cirrhosis of different etiologies were assayed for ANCA of IgG, IgA, IgA1, IgA2, and secretory IgA subtypes by indirect immunofluorescence and ELISAs. The control group comprised 202 patients with chronic liver diseases without cirrhosis and 100 healthy subjects. In cirrhosis, a 2-year follow-up, observational study was conducted to assess a possible association between the presence of ANCA and clinically significant bacterial infections.
  
  
  RESULTS
  Prevalence of ANCA IgA was significantly higher in cirrhosis (52.2%) compared to chronic liver diseases (18.6%) or healthy controls (0%, p<0.001 for both). ANCA IgA subtyping assays revealed marked increase in the proportion of IgA2 subtype (46% of total ANCA IgA) and presence of the secretory component concurrently. Presence of ANCA IgA was associated with disease-specific clinical characteristics (Child-Pugh stage and presence of ascites, p<0.001). During a 2-year follow-up period, risk of infections was higher among patients with ANCA IgA compared to those without (41.8% vs. 23.4%, p<0.001). ANCA IgA positivity was associated with a shorter time to the first infectious complication (pLogRank <0.001) in Kaplan-Meier analysis and was identified as an independent predictor in multivariate Cox-regression analysis (HR:1.74, 95% CI: 1.18-2.56, p=0.006).
  
  
  CONCLUSIONS
  Presence of IgA type ANCA is common in cirrhosis. Involvement of gut mucosal immune system is in center of their formation and probably reflects sustained exposure to bacterial constituents.
authors: 2nd Department of Medicine, Division of Gastroenterology, University of Debrecen, Debrecen, Hungary. papp.maria@med.unideb.hu
authors: Maria
authors: M
authors: Papp
authors: Nora
authors: N
authors: Sipeki
authors: Zsuzsanna
authors: Z
authors: Vitalis
authors: Tamas
authors: T
authors: Tornai
authors: Istvan
authors: I
authors: Altorjay
authors: Istvan
authors: I
authors: Tornai
authors: Miklos
authors: M
authors: Udvardy
authors: Kai
authors: K
authors: Fechner
authors: Silvia
authors: S
authors: Jacobsen
authors: Bianca
authors: B
authors: Teegen
authors: Andrea
authors: A
authors: Sumegi
authors: Gabor
authors: G
authors: Veres
authors: Peter Laszlo
authors: PL
authors: Lakatos
authors: Janos
authors: J
authors: Kappelmayer
authors: Peter
authors: P
authors: Antal-Szalmas
mesh_terms: D000328:Adult; D000368:Aged; D019268:Antibodies, Antineutrophil Cytoplasmic / Q000097:blood*; D001424:Bacterial Infections / Q000209:etiology* / Q000276:immunology*; D016022:Case-Control Studies; D005260:Female; D019698:Hepatitis C, Chronic / Q000150:complications / Q000276:immunology; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood* / Q000145:classification; D008103:Liver Cirrhosis / Q000150:complications* / Q000276:immunology*; D008104:Liver Cirrhosis, Alcoholic / Q000150:complications / Q000276:immunology; D008105:Liver Cirrhosis, Biliary / Q000150:complications / Q000276:immunology; D008107:Liver Diseases / Q000150:complications / Q000276:immunology; D008297:Male; D008875:Middle Aged; D012307:Risk Factors; D013997:Time Factors
chemical_list: D019268:Antibodies, Antineutrophil Cytoplasmic; D007070:Immunoglobulin A
keywords: 95% CI; 95% confidence intervals; AMA; ANCA; ANOVA; APRIL; ASCA; Anti-neutrophil cytoplasmic antibodies; B cell activating factor; BAFF; BT; Bacterial infection; C-ANCA; CLD; CRP; CSI; Cirrhosis; ELISA; HR; IIF; IL; IQR; LBP; MELD; MPO; OMP; OR; P-ANCA; PBC; PCT; PMN; PPI; PR-3; SBP; SC; SD; TBB-5; TGF; TLR; TNFα; a proliferation-inducing ligand; anti-PPS; anti-Saccharomyces cerevisiae antibodies; anti-neutrophil cytoplasmic antibody; anti-pneumococcal polysaccharide antibody; antimitochondrial antibodies; bacterial translocation; chronic HCV; chronic hepatitis C virus; chronic liver diseases; clinically significant bacterial infection; cytoplasmic anti-neutrophil cytoplasmic antibody; enzyme-linked immunosorbent assay; hazard ratio; high-sensitivity C reactive protein; human β tubulin isotype-5; iNOS; indirect immunofluorescence; inducible nitric oxide synthase; interleukin; interquartile range; mIgA; model for end-stage liver disease; monomeric immunoglobulin A; myeloperoxidase; odds ratio; one-way analysis of variance; outer membrane protein; pIgA; perinuclear anti-neutrophil cytoplasmic antibody; polymeric immunoglobulin A; polymorphonuclear cell; primary biliary cirrhosis; procalcitonin; proteinase-3; proton pump inhibitor; sCD89; sIgA; secretory IgA; secretory component; serum lipopolysaccharide-binding protein; soluble CD89; spontaneous bacterial peritonitis; standard deviation; toll-like receptor; transforming growth factor; tumor necrosis factor alpha