title: Oxidized low density lipoprotein induced caspase-1 mediated pyroptotic cell death in macrophages: implication in lesion instability?
abstract: BACKGROUND
  Macrophage death in advanced lesion has been confirmed to play an important role in plaque instability. However, the mechanism underlying lesion macrophage death still remains largely unknown.
  
  
  RESULTS
  Immunohistochemistry showed that caspase-1 activated in advanced lesion and co-located with macrophages and TUNEL positive reaction. In in-vitro experiments showed that ox-LDL induced caspase-1 activation and this activation was required for ox-LDL induced macrophages lysis, IL-1β and IL-18 production as well as DNA fragmentation. Mechanism experiments showed that CD36 and NLRP3/caspase-1/pathway involved in ox-LDL induced macrophage pyroptosis.
  
  
  CONCLUSIONS
  Our study here identified a novel cell death, pyroptosis in ox-LDL induced human macrophage, which may be implicated in lesion macrophages death and play an important role in lesion instability.
authors: Department of Cardiovascular Medicine, Shaanxi Provincial People's Hospital, Xi'an, China.
authors: Jing
authors: J
authors: Lin
authors: Xiling
authors: X
authors: Shou
authors: Xiaobo
authors: X
authors: Mao
authors: Jiangchuan
authors: J
authors: Dong
authors: Nilesh
authors: N
authors: Mohabeer
authors: Kishan Kumar
authors: KK
authors: Kushwaha
authors: Lei
authors: L
authors: Wang
authors: Yousu
authors: Y
authors: Su
authors: Hongcheng
authors: H
authors: Fang
authors: Dazhu
authors: D
authors: Li
mesh_terms: D053468:CARD Signaling Adaptor Proteins; D018955:CD36 Antigens / Q000378:metabolism; D002352:Carrier Proteins / Q000378:metabolism; D020170:Caspase 1 / Q000378:metabolism*; D016923:Cell Death / Q000187:drug effects; D003598:Cytoskeletal Proteins / Q000378:metabolism; D053938:DNA Fragmentation / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D006801:Humans; D020382:Interleukin-18 / Q000096:biosynthesis; D053583:Interleukin-1beta / Q000096:biosynthesis; D008077:Lipoproteins, LDL / Q000494:pharmacology*; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; D058226:Plaque, Atherosclerotic / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D053468:CARD Signaling Adaptor Proteins; D018955:CD36 Antigens; D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; D020382:Interleukin-18; D053583:Interleukin-1beta; D008077:Lipoproteins, LDL; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C453881:NLRP3 protein, human; C402202:PYCARD protein, human; D017382:Reactive Oxygen Species; C099252:oxidized low density lipoprotein; D020170:Caspase 1
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