title: An endovascular canine stroke model: middle cerebral artery occlusion with autologous clots followed by ipsilateral internal carotid artery blockade.
abstract: Stroke is one of the leading causes of death worldwide and the main reason for long-term disability. An appropriate animal model of stroke is urgently required for understanding the exact pathophysiological mechanism of stroke and testing any new therapeutic regimen. Our work aimed to establish a canine stroke model occluding the middle cerebral artery (MCA) and blocking the ipsilateral internal carotid artery (ICA), and to assess the infarct lesions by magnetic resonance imaging. The stroke model was generated by injecting two autologous clots into each MCA, followed by 2-h ipsilateral ICA blockade (ilICAB) using a catheter in 15 healthy adult beagles. Outcome measurements included 24-h and 7-day postocclusion T2-weighted imaging (T2WI)-based infarct volume calculation. In addition, pial collateral score, canine neurobehavioral score and histopathologic results were documented. Out of 15 dogs, 12 with successful MCA occlusion (MCAO) and ilICAB survived 7 days without complications or casualties and MCA were reperfused at 7 days after occlusion. High signal intensity in the basal ganglia and cerebral cortex on T2WI was initially observed in each dog at 6 h after the procedure. The mean percentage hemispherical infarct volume corrected for edema in all dogs on T2WI at 24 h after occlusion was 12.99±1.57%, and the degree of variability was 12.08%. The infarct volumes at 24 h after occlusion correlated with pial collateral scores and canine neurobehavioral scores well. This canine stroke model with combined MCAO and ilICAB reported here were proven to be highly feasible and reproducible.
authors: Department of Radiology, The First Affiliated Hospital of Nanjing Medical University, Gulou District, Nanjing, Jiangsu, China.
authors: Qing-Quan
authors: QQ
authors: Zu
authors: Sheng
authors: S
authors: Liu
authors: Xiao-Quan
authors: XQ
authors: Xu
authors: Shan-Shan
authors: SS
authors: Lu
authors: Lei
authors: L
authors: Sun
authors: Hai-Bin
authors: HB
authors: Shi
mesh_terms: D000792:Angiography; D000818:Animals; D001921:Brain / Q000473:pathology; D002343:Carotid Artery, Internal; D004195:Disease Models, Animal; D004285:Dogs; D008279:Magnetic Resonance Imaging; D008297:Male; D020768:Middle Cerebral Artery / Q000000981:diagnostic imaging; D009460:Neurologic Examination; D020521:Stroke / Q000000981:diagnostic imaging / Q000473:pathology*