title: Selenium nanoparticle-enriched Lactobacillus brevis causes more efficient immune responses in vivo and reduces the liver metastasis in metastatic form of mouse breast cancer.
abstract: OBJECTIVE
  Selenium enriched Lactobacillus has been reported as an immunostimulatory agent which can be used to increase the life span of cancer bearing animals. Lactic acid bacteria can reduce selenium ions to elemental selenium nanoparticles (SeNPs) and deposit them in intracellular spaces. In this strategy two known immunostimulators, lactic acid bacteria (LAB) and SeNPs, are concomitantly administered for enhancing of immune responses in cancer bearing mice.
  
  
  METHODS
  Forty five female inbred BALB/c mice were divided into three groups of tests and control, each containing 15 mice. Test mice were orally administered with SeNP-enriched Lactobacillus brevis or Lactobacillus brevis alone for 3 weeks before tumor induction. After that the administration was followed in three cycles of seven days on/three days off. Control group received phosphate buffer saline (PBS) at same condition. During the study the tumor growth was monitored using caliper method. At the end of study the spleen cell culture was carried out for both NK cytotoxicity assay and cytokines measurement. Delayed type hypersensitivity (DTH) responses were also assayed after 72h of tumor antigen recall. Serum lactate dehydrogenase (LDH) and alkaline phosphatase (ALP) levels were measured, the livers of mice were removed and prepared for histopathological analysis.
  
  
  RESULTS
  High level of IFN-γ and IL-17 besides the significant raised in NK cytotoxicity and DTH responses were observed in SeNP-enriched L. brevis administered mice and the extended life span and decrease in the tumor metastasis to liver were also recorded in this group compared to the control mice or L.brevis alone administered mice.
  
  
  CONCLUSIONS
  Our results suggested that the better prognosis could be achieved by oral administration of SeNP-enriched L. brevis in highly metastatic breast cancer mice model.
authors: Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, Iran. Shahverd@sina.tums.ac.ir.
authors: Mohammad Hossein
authors: MH
authors: Yazdi
authors: Mehdi
authors: M
authors: Mahdavi
authors: Neda
authors: N
authors: Setayesh
authors: Mohammad
authors: M
authors: Esfandyar
authors: Ahmad Reza
authors: AR
authors: Shahverdi
references: World J Gastroenterol. 2005 Oct 7;11(37):5795-800
references: 16270387
references: Int Arch Allergy Immunol. 1998 Apr;115(4):278-87
references: 9566350
references: Vet Q. 1998;20 Suppl 3:S81-3
references: 9689733
references: Am J Clin Nutr. 2000 Feb;71(2):405-11
references: 10648252
references: J BUON. 2011 Jan-Mar;16(1):9-15
references: 21674844
references: N Engl J Med. 2006 Nov 2;355(18):1920-2
references: 17079767
references: Carbohydr Res. 2011 Oct 18;346(14):2213-21
references: 21802071
references: Microbiol Immunol. 2000;44(4):213-22
references: 10832963
references: Br J Nutr. 2010 Jul;104(2):227-32
references: 20193099
references: Biofactors. 2004;22(1-4):119-22
references: 15630265
references: BMC Immunol. 2011 Aug 11;12:46
references: 21834957
references: Clin Cancer Res. 1998 Aug;4(8):1943-8
references: 9717823
references: Environ Res. 1987 Apr;42(2):277-303
references: 3552651
references: Int Arch Allergy Immunol. 2004 Nov;135(3):205-15
references: 15467373
references: Biol Trace Elem Res. 2012 Oct;149(1):22-8
references: 22476951
references: J Am Coll Nutr. 2001 Feb;20(1):1-4
references: 11293463
references: Clin Chem. 2003 Apr;49(4):662-4
references: 12651824
references: Cancer Res. 1966 Aug;26(8):1787-800
references: 4288553
references: Vaccine. 2004 Jul 29;22(21-22):2925-35
references: 15246629
references: Int Immunopharmacol. 2007 Jun;7(6):864-70
references: 17466920
references: Cancer Invest. 1994;12(6):620-43
references: 7994598
references: Nutrition. 1998 Jul-Aug;14(7-8):585-94
references: 9684261
references: J Autoimmun. 2009 Nov-Dec;33(3-4):275-81
references: 19682859
references: Environ Health Perspect. 1982 Feb;43:21-5
references: 7037385
references: Biotechnol Appl Biochem. 2010 May 14;56(1):7-15
references: 20408816
references: Arzneimittelforschung. 2012 Nov;62(11):525-31
references: 22945771
references: Vaccine. 2000 May 22;18(23):2613-23
references: 10775795
references: Cell Mol Life Sci. 2000 Dec;57(13-14):1864-73
references: 11215513
references: Scand J Clin Lab Invest. 1976 Oct;36(6):505-11
references: 12554
references: Immunol Today. 1999 Sep;20(9):387-90
references: 10462737
references: Proc Natl Acad Sci U S A. 2005 Jul 19;102(29):10321-6
references: 15985548
references: Med Microbiol Immunol. 1999 Dec;188(3):111-6
references: 10776840