title: Predicting how cells spread and migrate: focal adhesion size does matter.
abstract: Efficient cell migration is central to the normal development of tissues and organs and is involved in a wide range of human diseases, including cancer metastasis, immune responses, and cardiovascular disorders. Mesenchymal migration is modulated by focal-adhesion proteins, which organize into large integrin-rich protein complexes at the basal surface of adherent cells. Whether the extent of clustering of focal-adhesion proteins is actually required for effective migration is unclear. We recently demonstrated that the depletion of major focal-adhesion proteins, as well as modulation of matrix compliance, actin assembly, mitochondrial activity, and DNA recombination, all converged into highly predictable, inter-related, biphasic changes in focal adhesion size and cell migration. Herein, we further discuss the role of focal adhesions in controlling cell spreading and test their potential role in cell migration.
authors: Johns Hopkins Physical Sciences - Oncology Center, The Johns Hopkins University, Baltimore, MD, USA.
authors: Dong-Hwee
authors: DH
authors: Kim
authors: Denis
authors: D
authors: Wirtz
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology*; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D022001:Focal Adhesions; D006801:Humans; D060589:Zyxin / Q000378:metabolism*
chemical_list: D003598:Cytoskeletal Proteins; D060589:Zyxin
keywords: cell migration; focal adhesions; high-throughput phenotyping; mechanosensing; systems biology
references: Nat Cell Biol. 2011 Nov 13;13(12):1457-65
references: 22081092
references: Anal Chem. 2004 Sep 15;76(18):5257-64
references: 15362881
references: PLoS One. 2009;4(2):e4411
references: 19198659
references: Nat Cell Biol. 2003 Aug;5(8):694-7
references: 12894175
references: Mol Biol Cell. 2005 Sep;16(9):4329-40
references: 16000373
references: J Cell Biol. 1990 Sep;111(3):1059-68
references: 2118142
references: J Phys Chem B. 2013 Apr 18;117(15):4081-8
references: 23531225
references: J Biol Chem. 2003 Mar 7;278(10):8516-25
references: 12496265
references: J Cell Sci. 2003 Dec 1;116(Pt 23):4695-705
references: 14600256
references: PLoS One. 2011;6(7):e22025
references: 21779367
references: Proc Natl Acad Sci U S A. 2011 Apr 26;108(17):6693-4
references: 21490296
references: J Cell Sci. 1997 Nov;110 ( Pt 22):2833-44
references: 9427291
references: Radiat Res. 1994 Feb;137(2):162-70
references: 7510893
references: Tissue Eng Part A. 2011 Mar;17(5-6):713-24
references: 20929283
references: Science. 2005 Nov 18;310(5751):1139-43
references: 16293750
references: FASEB J. 2013 Apr;27(4):1351-61
references: 23254340
references: Nat Commun. 2012 Mar 06;3:719
references: 22395610
references: Mutagenesis. 1999 Jan;14(1):87-93
references: 10474828
references: J Cell Biol. 2001 Jun 11;153(6):1175-86
references: 11402062
references: J Cell Sci. 2000 May;113 ( Pt 10):1677-86
references: 10769199
references: Biomacromolecules. 2002 Nov-Dec;3(6):1375-83
references: 12425679
references: Proc Natl Acad Sci U S A. 2008 Nov 25;105(47):18331-6
references: 19017792
references: Sci Rep. 2012;2:555
references: 22870384
references: J Cell Sci. 1999 Aug;112 ( Pt 16):2677-91
references: 10413676
references: Nat Rev Mol Cell Biol. 2009 Jan;10(1):21-33
references: 19197329
references: Exp Cell Res. 2000 Nov 25;261(1):25-36
references: 11082272
references: PLoS One. 2009 Sep 16;4(9):e7054
references: 19756147
references: J Biol Chem. 2009 Jul 3;284(27):18252-9
references: 19458087
references: Cell Motil Cytoskeleton. 2005 Jan;60(1):24-34
references: 15573414
references: Biophys J. 2011 Dec 21;101(12):2919-28
references: 22208190
references: Biol Cell. 2010 Jan 14;102(4):203-213
references: 19883375
references: J Cell Biol. 2000 May 29;149(5):1073-86
references: 10831611
references: Proc Natl Acad Sci U S A. 1997 Dec 9;94(25):13661-5
references: 9391082
references: Biophys J. 1991 Jul;60(1):15-37
references: 1883934
references: J Cell Sci. 2001 Mar;114(Pt 5):1025-36
references: 11181185
references: Nature. 1996 Jun 6;381(6582):531-5
references: 8632828
references: Cell Res. 2009 Feb;19(2):236-46
references: 19065153
references: Nat Cell Biol. 2008 Sep;10(9):1039-50
references: 19160484