title: Solution of the problem of nonspecific binding in solid-phase noninstrumental dot immunoassay.
abstract: In this work, we evaluated blocking properties of various reagents used in dot immunoassay test systems on nitrocellulose membranes employing carbon-based diagnosticum to determine antibodies to tetanus anatoxin, thermostable toxin of Yersinia pseudotuberculosis, and HIV-I. The results support the usage of a nonionic detergent Tween 20 which efficiently inhibited nonspecific protein binding to the solid-phase reagent surface and did not decrease sensitivity of the analysis.
authors: Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences, Perm, Russia. krasnykh-m@mail.ru
authors: M S
authors: MS
authors: Bochkova
authors: V P
authors: VP
authors: Timganova
authors: M B
authors: MB
authors: Raev
mesh_terms: D000914:Antibodies, Viral / Q000032:analysis / Q000737:chemistry / Q000276:immunology; D016477:Artifacts; D001427:Bacterial Toxins / Q000032:analysis / Q000737:chemistry / Q000276:immunology; D015497:HIV-1 / Q000276:immunology; D055767:Immobilized Proteins / Q000737:chemistry; D015151:Immunoblotting / Q000379:methods*; D007202:Indicators and Reagents / Q000737:chemistry; D008024:Ligands; D011485:Protein Binding; D015011:Yersinia pseudotuberculosis / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D001427:Bacterial Toxins; D055767:Immobilized Proteins; D007202:Indicators and Reagents; D008024:Ligands
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