title: Enhanced microRNA accumulation through stemloop-adjacent introns.
abstract: MicroRNAs (miRNAs) originate from stemloop-forming precursor RNAs found in longer primary transcripts that often contain introns. We show that in plants, those introns, when located 3' of the stemloop, can promote mature miRNA accumulation, through a mechanism that likely operates at the level of miRNA processing or stability. Reversely, when miRNA production is reduced such as in dicer-like 1 mutants, splicing of introns that promote miRNA processing is considerably increased, pointing to a tight physical and temporal coordination of intron splicing and miRNA processing in plants. Our findings further suggest that miRNA transcripts without introns generated through alternative polyA-site usage might contribute to the differential adjustment of miRNA levels, possibly at a tissue-specific level.
authors: Institut de Biologie Moléculaire des Plantes, UPR2357, Strasbourg 67084, France.
authors: Rebecca
authors: R
authors: Schwab
authors: Corinna
authors: C
authors: Speth
authors: Sascha
authors: S
authors: Laubinger
authors: Olivier
authors: O
authors: Voinnet
mesh_terms: D017398:Alternative Splicing; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D007438:Introns; D055029:Inverted Repeat Sequences; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009928:Organ Specificity; D011061:Poly A / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism
chemical_list: C577258:MIRN163 microRNA, Arabidopsis; D035683:MicroRNAs; C543702:Mirn172 microRNA, Arabidopsis; D012333:RNA, Messenger; D011061:Poly A
references: Science. 2008 Sep 12;321(5895):1490-2
references: 18787168
references: PLoS Genet. 2009 Nov;5(11):e1000716
references: 19936050
references: RNA. 2002 Nov;8(11):1444-53
references: 12458797
references: Plant Physiol. 2005 Aug;138(4):2145-54
references: 16040653
references: Genes Dev. 2004 Oct 1;18(19):2368-79
references: 15466488
references: Genes Dev. 2004 Sep 15;18(18):2237-42
references: 15371337
references: Trends Plant Sci. 2002 May;7(5):193-5
references: 11992820
references: Mol Cell. 2012 Nov 30;48(4):521-31
references: 23063528
references: Nucleic Acids Res. 2009 May;37(9):3083-93
references: 19304749
references: Cell. 2009 Feb 20;136(4):642-55
references: 19239886
references: Plant J. 2001 Sep;27(6):581-90
references: 11576441
references: EMBO J. 2007 Feb 7;26(3):775-83
references: 17255951
references: Plant Cell. 2011 Feb;23(2):431-42
references: 21317375
references: EMBO Rep. 2013 Jul;14(7):622-8
references: 23681439
references: Plant J. 2004 Dec;40(5):744-51
references: 15546357
references: Cell. 2009 Feb 20;136(4):701-18
references: 19239890
references: Mol Gen Genet. 1990 Jan;220(2):245-50
references: 2325623
references: RNA Biol. 2009 Nov-Dec;6(5):536-40
references: 19875945
references: Plant Cell. 2008 Mar;20(3):543-51
references: 18319396
references: Plant J. 2011 Oct;68(1):159-67
references: 21689169
references: Plant Cell Environ. 2008 Apr;31(4):562-74
references: 18208512
references: Curr Genomics. 2008 Jun;9(4):227-38
references: 19452040
references: Nature. 2000 Sep 21;407(6802):319-20
references: 11014180
references: Proc Natl Acad Sci U S A. 2004 Aug 24;101(34):12753-8
references: 15314213
references: Mol Cell Biol. 2009 Jun;29(12):3243-54
references: 19349299
references: Nat Genet. 2004 Dec;36(12):1282-90
references: 15565108