title: Mapping the ligand-binding region of Borrelia hermsii fibronectin-binding protein.
abstract: Many pathogenic microorganisms express fibronectin-binding molecules that facilitate their adherence to the extracellular matrix and/or entry into mammalian cells. We have previously described a Borrelia recurrentis gene, cihC that encodes a 40-kDa surface receptor for both, fibronectin and the complement inhibitors C4bp and C1-Inh. We now provide evidence for the expression of a group of highly homologues surface proteins, termed FbpA, in three B. hermsii isolates and two tick-borne relapsing fever spirochetes, B. parkeri and B. turicatae. When expressed in Escherichia coli or B. burgdorferi, four out of five proteins were shown to selectively bind fibronectin, whereas none of five proteins were able to bind the human complement regulators, C4bp and C1-Inh. By applying deletion mutants of the B. hermsii fibronectin-binding proteins a putative high-affinity binding site for fibronectin was mapped to its central region. In addition, the fibronectin-binding proteins of B. hermsii were found to share sequence homology with BBK32 of the Lyme disease spirochete B. burgdorferi with similar function suggesting its involvement in persistence and/or virulence of relapsing fever spirochetes.
authors: Infectious Immunology Group, Institute for Immunology, University of Heidelberg, Heidelberg, Germany.
authors: Christiane
authors: C
authors: Brenner
authors: Katharina
authors: K
authors: Bomans
authors: Jüri
authors: J
authors: Habicht
authors: Markus M
authors: MM
authors: Simon
authors: Reinhard
authors: R
authors: Wallich
mesh_terms: D018829:Adhesins, Bacterial / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D001898:Borrelia / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D008024:Ligands; D008969:Molecular Sequence Data; D010447:Peptide Hydrolases / Q000378:metabolism; D017434:Protein Structure, Tertiary; D012689:Sequence Homology, Nucleic Acid
chemical_list: D018829:Adhesins, Bacterial; D005353:Fibronectins; D008024:Ligands; C078635:fibronectin-binding proteins, bacterial; D010447:Peptide Hydrolases
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