title: Adaptor protein complexes-1 and 3 are involved at distinct stages of flavivirus life-cycle.
abstract: Intracellular protein trafficking pathways are hijacked by viruses at various stages of viral life-cycle. Heterotetrameric adaptor protein complexes (APs) mediate vesicular trafficking at distinct intracellular sites and are essential for maintaining the organellar homeostasis. In the present study, we studied the effect of AP-1 and AP-3 deficiency on flavivirus infection in cells functionally lacking these proteins. We show that AP-1 and AP-3 participate in flavivirus life-cycle at distinct stages. AP-3-deficient cells showed delay in initiation of Japanese encephalitis virus and dengue virus RNA replication, which resulted in reduction of infectious virus production. AP-3 was found to colocalize with RNA replication compartments in infected wild-type cells. AP-1 deficiency affected later stages of dengue virus infection where increased intracellular accumulation of infectious virus was observed. Therefore, our results propose a novel role for AP-1 and AP-3 at distinct stages of infection of some of the RNA viruses.
authors: Vaccine and Infectious Disease Research Center, Translational Health Science and Technology Institute, Gurgaon, India.
authors: Tanvi
authors: T
authors: Agrawal
authors: Peter
authors: P
authors: Schu
authors: Guruprasad R
authors: GR
authors: Medigeshi
mesh_terms: D033961:Adaptor Protein Complex 1 / Q000502:physiology*; D033963:Adaptor Protein Complex 3 / Q000502:physiology*; D000818:Animals; D015153:Blotting, Western; D049109:Cell Proliferation; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003715:Dengue / Q000378:metabolism / Q000473:pathology / Q000821:virology; D003716:Dengue Virus / Q000502:physiology; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism / Q000821:virology; D004664:Encephalitis Virus, Japanese / Q000502:physiology; D004672:Encephalitis, Japanese / Q000378:metabolism / Q000473:pathology / Q000821:virology; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism / Q000821:virology; D005416:Flavivirus / Q000502:physiology*; D018177:Flavivirus Infections / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D005455:Fluorescent Antibody Technique; D007668:Kidney / Q000166:cytology / Q000378:metabolism / Q000821:virology; D051379:Mice; D018345:Mice, Knockout; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014709:Vero Cells; D014779:Virus Replication
chemical_list: D033961:Adaptor Protein Complex 1; D033963:Adaptor Protein Complex 3; D012333:RNA, Messenger; D012367:RNA, Viral
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