title: [The effect of niflumic acid on gamma aminobutyric acid activated current in DRG neurons].
abstract: OBJECTIVE
  To explore the modulatory effect of niflumic acid (NFA) on gamma aminobutyric acid (GABA)-activated currents of dorsal root ganglion (DRG) neurons in rat.
  
  
  METHODS
  The whole-cell patch-clamp technique was used to record the NFA- and GABA-activated currents in neurons freshly dissociated from rat DRG neurons.
  
  
  RESULTS
  Application of NFA(0.1 - 100 micromol/L) could induce concentration-dependent outward currents in some cells (21/48,43.75%), and GABA (0.1 - 100 micromol/L) could induce concentration-dependent inward currents in some cells(150/159,94.32%). NFA-(100 micromol/L) and GABA-(100 micromol/L) activated currents were (0.27 +/- 0.06) nA (n = 12) and (1.29 +/- 0.72) nA (n = 53) respectively. However, pre-application of NFA (0.1 - 100 micromol/L) could inhibit the GABA-activated inward current which was identified to be GABAA receptor-mediated current. The inhibitory effects of NFA were concentration-dependent. NFA could not alter the EC50 (about 30 micromol/L) and inverse potential (about -10 mV) of GABA-activated current (P > 0.05).
  
  
  CONCLUSIONS
  Pre-application of NFA exerts a more strong inhibitory effect on the peak value of GABA-activated current.
authors: Department of Physiology, Medical College, Shihezi University, Shihezi 832002, China.
authors: Li
authors: L
authors: Li
authors: Jing
authors: J
authors: Li
authors: Ke-Tao
authors: KT
authors: Ma
authors: Hong-Ju
authors: HJ
authors: Cheng
authors: Lei
authors: L
authors: Zhao
authors: Yang
authors: Y
authors: Wang
authors: Jun-Qiang
authors: JQ
authors: Si
mesh_terms: D000818:Animals; D002469:Cell Separation; D002478:Cells, Cultured; D005727:Ganglia, Spinal / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000187:drug effects* / Q000502:physiology*; D009544:Niflumic Acid / Q000494:pharmacology*; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D009544:Niflumic Acid; D005680:gamma-Aminobutyric Acid