title: Novel gene expression model for outcome prediction in paediatric medulloblastoma.
abstract: Medulloblastoma is the most frequent type of embryonal tumour in the paediatric population. The disease progression in patients with this tumour may be connected with the presence of stem/tumour-initiating cells, but the precise source and characteristics of such cells is still a subject of debate. Thus, we tried to analyse biomarkers for which a connection with the presence of stem/tumour-initiating cells was suggested. We evaluated the transcriptional level of the ATOH1, FUT4, NGFR, OTX1, OTX2, PROM1 and SOX1 genes in 48 samples of medulloblastoma and analysed their usefulness in the prediction of disease outcome. The analyses showed a strong correlation of PROM1, ATOH1 and OTX1 gene expression levels with the outcome (p ≤ 0.2). On the basis of the multivariate Cox regression analysis, we propose a three-gene model predicting risk of the disease, calculated as follows: RS(risk score) =( 0:81 x PROM1) + (0:18 x OTX1) + (0:02 x ATOH1). Survival analysis revealed a better outcome among standard-risk patients, with a 5-year survival rate of 65 %, compared to the 40 % rate observed among high-risk patients. The most promising advantage of such molecular analysis consists in the identification of molecular markers influencing clinical behaviour, which may in turn be useful in therapy optimization.
authors: Department of Molecular Pathology and Neuropathology, Chair of Oncology, Medical University of Lodz, Czechoslowacka 8/10 str, 92-216, Lodz, Poland, magdalena.zakrzewska@umed.lodz.pl.
authors: Magdalena
authors: M
authors: Zakrzewska
authors: Sylwia M
authors: SM
authors: Grešner
authors: Krzysztof
authors: K
authors: Zakrzewski
authors: Beata
authors: B
authors: Zalewska-Szewczyk
authors: Pawel P
authors: PP
authors: Liberski
mesh_terms: D000071916:AC133 Antigen; D000293:Adolescent; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism*; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005647:Fucosyltransferases / Q000235:genetics / Q000378:metabolism; D006023:Glycoproteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007223:Infant; D016256:Lewis X Antigen / Q000235:genetics / Q000378:metabolism; D008297:Male; D008527:Medulloblastoma / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D009373:Neoplasms, Germ Cell and Embryonal / Q000175:diagnosis / Q000235:genetics* / Q000378:metabolism; D014411:Neoplastic Stem Cells / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D051857:Otx Transcription Factors / Q000235:genetics / Q000378:metabolism*; D010455:Peptides / Q000235:genetics / Q000378:metabolism*; D011379:Prognosis; D017475:Receptors, Nerve Growth Factor / Q000235:genetics / Q000378:metabolism; D055748:SOXB1 Transcription Factors / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D051792:Basic Helix-Loop-Helix Proteins; D005647:Fucosyltransferases; D006023:Glycoproteins; D016256:Lewis X Antigen; D009419:Nerve Tissue Proteins; D051857:Otx Transcription Factors; D010455:Peptides; D017475:Receptors, Nerve Growth Factor; D055748:SOXB1 Transcription Factors; C500942:ATOH1 protein, human; C484065:NGFR protein, human; C497655:OTX1 protein, human; C497659:OTX2 protein, human; C000606023:PROM1 protein, human; C490906:SOX1 protein, human; C066693:FUT4 protein, human
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