title: High-throughput quality control of DMSO acoustic dispensing using photometric dye methods.
abstract: One high-throughput technology gaining widespread adoption in industry and academia is acoustic liquid dispensing, in which focused sound waves eject nanoliter-sized droplets from a solution into a recipient microplate. This technology allows for direct dispensing of small-molecule compounds or reagents dissolved in DMSO, while keeping a low final concentration of organic solvent in an assay. However, acoustic dispensing presents unique quality control (QC) challenges when measuring the accuracy and precision of small dispense volumes ranging from 2.5 to 100 nL. As part of an effort to develop a rapid and cost-effective QC method for acoustic dispensing of 100% DMSO, we implemented the first high-throughput photometric dual-dye-based QC protocol in the nanoliter volume range. This technical note validates the new photometric 100% DMSO QC method and highlights its cost-effectiveness when compared with conventional low-throughput fluorimetric QC methods. In addition, a potential software solution is described for the analysis, storage, and display of accumulated high-throughput QC data, called LabGauge. As the need for high-throughput QC grows, conventional low-throughput methods can no longer meet demand. Validated high-throughput techniques, such as the dual-dye photometric method, will need to be implemented.
authors: Merck & Co., Inc., Merck Research Laboratories-Boston, In Vitro Pharmacology, Boston, MA, USA. catherine_quintero@merck.com
authors: Catherine
authors: C
authors: Quintero
authors: Kristen
authors: K
authors: Tran
authors: Alexander A
authors: AA
authors: Szewczak
mesh_terms: D003362:Cost-Benefit Analysis; D004121:Dimethyl Sulfoxide / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D015590:Fluorophotometry / Q000295:instrumentation / Q000379:methods*; D057166:High-Throughput Screening Assays; D006801:Humans; D056656:Lab-On-A-Chip Devices; D008832:Microchemistry; D044085:Microfluidics / Q000379:methods / Q000639:trends; D008904:Miniaturization; D036103:Nanotechnology; D011786:Quality Control; D012984:Software; D013016:Sound
chemical_list: D005456:Fluorescent Dyes; D004121:Dimethyl Sulfoxide
keywords: Alexa Fluor; acoustic dispensing; dual-dye photometric; quality control