title: Multifunctional magnetic nanoparticles modified with polyethylenimine and folic acid for biomedical theranostics.
abstract: This paper describes the preparation of magnetic nanoparticles modified with polyethylenimine (PEI)-folic acid (PF) conjugate and their potential biomedical applications. Magnetic nanoparticles modified with (3-(2-aminoethylamino)propyltrimethoxysilane) (AEAPS) were first prepared using a ligand exchange method to provide biocompatibility and hydrophilicity, and further conjugated with PF to carry gene and enhance specific uptake into cancer cells. We demonstrated the feasibility of the multifunctional magnetic nanoparticles as contrast agents in magnetic resonance imaging (MRI) and as gene carriers for gene delivery. In vitro results revealed that the cytotoxicity of the multifunctional magnetic nanoparticles was lower compared to that of pristine magnetic nanoparticles. Furthermore, we demonstrated the specific uptake of the magnetic nanoparticles modified with PF to KB cells using WI-38 cells as comparison by confocal microscopy. The PF-modified magnetic nanoparticles can potentially be employed as theranostic nanoplatforms for targeted gene delivery to cancer cells and simultaneous magnetic resonance imaging.
authors: Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul, Republic of Korea.
authors: Hyunhee
authors: H
authors: Yoo
authors: Seung-Kwan
authors: SK
authors: Moon
authors: Taewon
authors: T
authors: Hwang
authors: Yong Seok
authors: YS
authors: Kim
authors: Joo-Hwan
authors: JH
authors: Kim
authors: Sung-Wook
authors: SW
authors: Choi
authors: Jung Hyun
authors: JH
authors: Kim
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005492:Folic Acid / Q000737:chemistry* / Q000494:pharmacology; D018014:Gene Transfer Techniques; D006801:Humans; D007624:KB Cells; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011094:Polyethyleneimine / Q000737:chemistry* / Q000494:pharmacology; D013329:Structure-Activity Relationship; D013499:Surface Properties
chemical_list: D058185:Magnetite Nanoparticles; D011094:Polyethyleneimine; D005492:Folic Acid