title: [Construction and assessment of heart-speciﬁc green ﬂuorescence zebrafish line].
abstract: Using the promoter for cardiac myosin light chain 2 (cmlc2) gene, an expression vector pTol2-cmlc2-IRES- EGFP for making heart-specific expression of exogenous gene in transgenic zebrafish was generated previously. Here, we reported the construction of a transgenic zebrafish line which stably expresses EGFP using this vector, and the effects of EGFP on the heart development and cardiac function of this transgenic zebrafish line were preliminarily analyzed. The results showed that the green fluorescence signal of cmlc2:EGFP line under fluorescence microscopy specifically expressed in heart and faithfully recapitulated both the spatial and temporal expression patterns of endogenous cmlc2 gene revealed by in situ hybridization in the early developmental stages. The cardiac morphology and development of this transgenic zebrafish line remained to be normal. Furthermore, the heart morphology and physiological function of this transgenic line have been analyzed using M-mode analysis. The results showed that there was no significant difference between the cmlc2:EGFP and the wild type lines with respect to heart period, heart rate, diastolic surface area and systolic surface area, and fractional area change. No tachyarrhythmia was observed in the embryos from either line. Thus, the excessive expression of EGFP in this transgenic line seemed to exert no detrimental effects on the function and development of zebrafish hearts during early stages. Our study laid a foundation for the construction of exogenous gene transgenic line using pTol2-cmlc2-IRES-EGFP vector to study the function of genes that expressed in heart.
authors: Center for Heart Development, Hunan Normal University, Chang-sha, China. XiyangPeng27@gmail.com
authors: Xi-Yang
authors: XY
authors: Peng
authors: Ting-Fang
authors: TF
authors: Chen
authors: Ting
authors: T
authors: Huang
authors: Zhi-Gang
authors: ZG
authors: Jiang
authors: Xiu-Shan
authors: XS
authors: Wu
authors: Yun
authors: Y
authors: Deng
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D005786:Gene Expression Regulation; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics*; D006321:Heart / Q000502:physiology; D009206:Myocardium / Q000378:metabolism*; D018994:Myosin Light Chains / Q000235:genetics; D009928:Organ Specificity; D015027:Zebrafish / Q000235:genetics* / Q000502:physiology; D029961:Zebrafish Proteins / Q000235:genetics
chemical_list: D018994:Myosin Light Chains; D029961:Zebrafish Proteins; C485184:enhanced green fluorescent protein; C532159:myl7 protein, zebrafish; D049452:Green Fluorescent Proteins