title: Integrin α3 is overexpressed in glioma stem-like cells and promotes invasion.
abstract: BACKGROUND
  Glioma stem-like cell (GSC) properties are responsible for gliomagenesis and recurrence. GSCs are invasive but its mechanism remains to be elucidated. Here, we attempted to identify the molecules that promote invasion in GSCs.
  
  
  METHODS
  Neurospheres and CD133⁺ cells were collected from glioblastoma (GBM) specimens and glioma cell lines by sphere-formation method and magnetic affinity cell sorting, respectively. Differential expression of gene candidates, its role in invasion and its signaling pathway were evaluated in glioma cell lines.
  
  
  RESULTS
  Neurospheres from surgical specimens attached to fibronectin and laminin, the receptors of which belong to the integrin family. Integrin α3 was overexpressed in CD133⁺ cells compared with CD133⁻ cells in all the glioma cell lines (4 out of 4). Immunohistochemistry demonstrated the localisation of integrin α3 in GBM cells, including invading cells, and in the tumour cells around the vessels, which is believed to be a stem cell niche. The expression of integrin α3 was correlated with migration and invasion. The invasion activity of glioma cells was linked to the phosphorylation of extracellular signal-regulated kinase (ERK) 1/2.
  
  
  CONCLUSIONS
  Our results suggest that integrin α3 contributes to the invasive nature of GSCs via ERK1/2, which renders integrin α3 a prime candidate for anti-invasion therapy for GBM.
authors: Department of Neurosurgery, Graduate School of Medical Science, Kanazawa University, Kanazawa 920 8641, Japan. mnakada@med.kanazawa-u.ac.jp
authors: M
authors: M
authors: Nakada
authors: E
authors: E
authors: Nambu
authors: N
authors: N
authors: Furuyama
authors: Y
authors: Y
authors: Yoshida
authors: T
authors: T
authors: Takino
authors: Y
authors: Y
authors: Hayashi
authors: H
authors: H
authors: Sato
authors: Y
authors: Y
authors: Sai
authors: T
authors: T
authors: Tsuji
authors: K-i
authors: KI
authors: Miyamoto
authors: A
authors: A
authors: Hirao
authors: J-i
authors: JI
authors: Hamada
mesh_terms: D001932:Brain Neoplasms / Q000235:genetics / Q000473:pathology*; D002448:Cell Adhesion / Q000235:genetics; D002465:Cell Movement / Q000235:genetics; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics / Q000473:pathology*; D006801:Humans; D039422:Integrin alpha3 / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D020935:MAP Kinase Signaling System / Q000502:physiology; D009361:Neoplasm Invasiveness; D014411:Neoplastic Stem Cells / Q000378:metabolism* / Q000473:pathology; D010766:Phosphorylation; D014407:Tumor Cells, Cultured; D015854:Up-Regulation / Q000235:genetics
chemical_list: C067625:FN1 protein, human; D005353:Fibronectins; D039422:Integrin alpha3; D048049:Extracellular Signal-Regulated MAP Kinases
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