title: Determination of rimantadine in human urine by HPLC using a monolithic stationary phase and on-line post-column derivatization.
abstract: In the present study, we propose the first HPLC method coupled to postcolumn derivatization for the determination of rimantadine in human urine samples. The analyte and amantadine (internal standard) were isocratically separated using an RP monolithic stationary phase (100 × 4.6 mm id) with a mobile phase consisting of CH3OH/phosphate buffer (25 mmol/L, pH 3.0) at a volume ratio of 50:50. Postcolumn derivatization involved on-line reaction with o-phthalaldehyde (20 mmol/L) and N-acetyl-cysteine (5 mmol/L) at alkaline medium (100 mmol/L borate pH 11.0). Spectrofluorimetric detection at λ(ex)/λ(em) = 340/455 nm enabled the selective and sensitive determination of rimantadine in urine samples at a range of 50-500 ng/mL with an LOD of 5 ng/mL. Human urine samples were analyzed successfully after SPE using hydrophilic-lipophilic balanced RP cartridges (30 mg/mL, Oasis HLB). Recoveries ranged between 89.7 and 102.7%.
authors: Laboratory of Analytical Chemistry, Department of Chemistry, University of Ioannina, Ioannina, Greece.
authors: Constantinos K
authors: CK
authors: Zacharis
authors: Paraskevas D
authors: PD
authors: Tzanavaras
authors: Athanasios G
authors: AG
authors: Vlessidis
mesh_terms: D001331:Automation / Q000295:instrumentation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D064368:Healthy Volunteers; D006801:Humans; D012299:Rimantadine / Q000652:urine*; D012680:Sensitivity and Specificity
chemical_list: D012299:Rimantadine