title: Fine mapping TaFLW1, a major QTL controlling flag leaf width in bread wheat (Triticum aestivum L.).
abstract: BACKGROUND
  Flag leaf width (FLW) is directly related to photosynthetic capacity and yield potential in wheat. In a previous study, Qflw.nau-5A controlling FLW was detected on chromosome 5A in the interval possessing Fhb5 for type I Fusarium head blight (FHB) resistance using a recombinant inbred line population derived from Nanda2419 × Wangshuibai.
  
  
  METHODS
  Qflw.nau-5A near-isogenic line (NIL) with the background of Mianyang 99-323 and PH691 was developed and evaluated. FLW inheritance was investigated using two F2 populations developed from crossing the Qflw.nau-5A NILs with their recurrent parents. One hundred ten and 28 recombinants, which included 10 and 5 types of recombinants, were identified from 2816 F2 plants with Mianyang 99-323 background and 1277 F2 plants with PH691 background, respectively, and phenotyped in field trials for FLW and type I FHB resistance. Deletion bin mapping was applied to physically map Qflw.nau-5A.
  
  
  CONCLUSIONS
  The introduction of Wangshuibai Qflw.nau-5A allele reduced the FLW up to 3 mm. In the F2 populations, Qflw.nau-5A was inherited like a semi-dominant gene, and was therefore designated as TaFLW1. The FLW of the recombinant lines displayed a distinct two-peak distribution. Recombinants with wider leaves commonly have Mianyang 99-323 or PH691 chromatin in the 0.2 cM Xwmc492-Xwmc752 interval that resided in the 5AL12-0.35-0.57 deletion bin, and recombinants with narrow leaves were Wangshuibai genotype in this interval. Phenotypic recombination between FLW and type I FHB resistance was identified, implying TaFLW1 was in close linkage with Fhb5. These results should aid wheat breeders to break the linkage drag through marker-assisted selection and assist in the map-based cloning of TaFLW1.
authors: Applied Plant Genomics Laboratory, Crop Genomics and Bioinformatics Centre and National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, Jiangsu, China.
authors: Shulin
authors: S
authors: Xue
authors: Feng
authors: F
authors: Xu
authors: Guoqiang
authors: G
authors: Li
authors: Yan
authors: Y
authors: Zhou
authors: Musen
authors: M
authors: Lin
authors: Zhongxia
authors: Z
authors: Gao
authors: Xiuhong
authors: X
authors: Su
authors: Xiaowu
authors: X
authors: Xu
authors: Ge
authors: G
authors: Jiang
authors: Shuang
authors: S
authors: Zhang
authors: Haiyan
authors: H
authors: Jia
authors: Zhongxin
authors: Z
authors: Kong
authors: Lixia
authors: L
authors: Zhang
authors: Zhengqiang
authors: Z
authors: Ma
mesh_terms: D001939:Bread; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D060467:Disease Resistance / Q000235:genetics*; D005670:Fusarium / Q000276:immunology*; D017353:Gene Deletion; D008040:Genetic Linkage; D005819:Genetic Markers / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D007113:Immunity, Innate; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology; D018515:Plant Leaves / Q000033:anatomy & histology / Q000235:genetics; D040641:Quantitative Trait Loci / Q000235:genetics*; D014908:Triticum / Q000235:genetics* / Q000382:microbiology*
chemical_list: D005819:Genetic Markers
references: Mol Genet Genomics. 2008 May;279(5):499-507
references: 18293011
references: Theor Appl Genet. 2005 Mar;110(5):865-80
references: 15719212
references: Yi Chuan Xue Bao. 2006 Sep;33(9):824-32
references: 16980129
references: Theor Appl Genet. 2009 Feb;118(3):581-90
references: 19020855
references: Plant Physiol. 2008 Aug;147(4):1947-59
references: 18562767
references: Theor Appl Genet. 2006 Feb;112(3):528-35
references: 16328229
references: Yi Chuan. 2003 May;25(3):317-21
references: 15639879
references: Cytogenet Genome Res. 2010 Jul;129(1-3):211-23
references: 20501977
references: Theor Appl Genet. 2003 Jun;107(1):89-101
references: 12721635
references: Nat Genet. 2012 Jun 24;44(8):950-4
references: 22729225
references: Theor Appl Genet. 2008 Apr;116(6):789-96
references: 18219477
references: J Integr Plant Biol. 2010 Nov;52(11):996-1007
references: 20977657
references: Theor Appl Genet. 2011 Oct;123(6):1055-63
references: 21739138
references: Plant Cell Rep. 2012 May;31(5):863-72
references: 22179305
references: Theor Appl Genet. 2011 Dec;123(8):1319-30
references: 21830109
references: Theor Appl Genet. 2012 Dec;125(8):1717-26
references: 22864386
references: Theor Appl Genet. 2003 Apr;106(6):1032-40
references: 12671751
references: Theor Appl Genet. 2004 Oct;109(6):1105-14
references: 15490101
references: Theor Appl Genet. 2005 Feb;110(3):550-60
references: 15655666
references: Theor Appl Genet. 2013 Aug;126(8):2123-39
references: 23689745
references: Theor Appl Genet. 2003 Feb;106(4):649-58
references: 12595994
references: J Integr Plant Biol. 2010 Jun;52(6):578-84
references: 20590988
references: Genetics. 1998 Aug;149(4):2007-23
references: 9691054
references: Nature. 1999 Jul 15;400(6741):256-61
references: 10421366
references: Anal Biochem. 1991 Jul;196(1):80-3
references: 1716076
references: J Exp Bot. 2012 Jul;63(12):4419-36
references: 22791821
references: J Zhejiang Univ Sci B. 2008 Dec;9(12):938-43
references: 19067461
references: Genetics. 2001 Jul;158(3):1363-79
references: 11454782
references: Genome. 1996 Feb;39(1):123-30
references: 8851802
references: Theor Appl Genet. 2008 Jul;117(2):181-9
references: 18437345