title: Development of an LC/MS/MS method in order to determine arctigenin in rat plasma: its application to a pharmacokinetic study.
abstract: In this study, a simple and sensitive LC/MS/MS method was developed and validated for the determination of arctigenin in rat plasma. The MS detection was performed using multiple reaction monitoring at the transitions of m/z 373.2 → 137.3 for arctigenin and m/z 187.1 → 131.0 for psoralen (internal standard) with a Turbo IonSpray electrospray in positive mode. The calibration curves fitted a good linear relationship over the concentration range of 0.2-500 ng/mL. It was found that arctigenin is not stable enough at both room temperature and -80 °C unless mixed with methanol before storage. The validated LC/MS/MS method was successfully applied for the pharmacokinetic study of arctigenin in rats. After intravenous injection of 0.3 mg/kg arctigenin injection to rats, the maximum concentration, half-life and area under the concentration-time curve were 323 ± 65.2 ng/mL, 0.830 ± 0.166 and 81.0 ± 22.1 h ng/mL, respectively.
authors: Key Laboratory of Systems Bioengineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
authors: Quanfei
authors: Q
authors: Zou
authors: Yuan
authors: Y
authors: Gu
authors: Rong
authors: R
authors: Lu
authors: Tiejun
authors: T
authors: Zhang
authors: Guang-Rong
authors: GR
authors: Zhao
authors: Changxiao
authors: C
authors: Liu
authors: Duanyun
authors: D
authors: Si
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D004355:Drug Stability; D005663:Furans / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D017705:Lignans / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D057230:Limit of Detection; D051381:Rats; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D005663:Furans; D017705:Lignans; C071942:arctigenin
keywords: LC/MS/MS; arctigenin; pharmacokinetics; rat plasma