title: Real-time polymerase chain reaction for detection of encapsulated Haemophilus influenzae using degenerate primers to target the capsule transport gene bexA.
abstract: A real-time polymerase chain reaction assay that uses degenerate primers and a dual-labelled probe was developed to detect the bexA gene of Haemophilus influenzae, including those belonging to non-b serotypes as well as clonal division II strains. This assay is sensitive and specific, detecting 20 copies of the gene, but negative with a variety of bacteria associated with meningitis and bacteremia or septicemia.
authors: Vaccine Preventable Bacterial Diseases, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, MB R3E 2R2, Canada.
authors: Dennis K S
authors: DK
authors: Law
authors: Raymond S W
authors: RS
authors: Tsang
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D002555:Cerebrospinal Fluid / Q000382:microbiology; D017931:DNA Primers / Q000235:genetics; D006192:Haemophilus Infections / Q000175:diagnosis* / Q000382:microbiology; D006193:Haemophilus influenzae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D019741:Haemophilus influenzae type b / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007223:Infant; D008581:Meningitis / Q000382:microbiology; D016133:Polymerase Chain Reaction; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; D017931:DNA Primers; C068101:bexA protein, Haemophilus influenzae