title: Immunogenicity analysis following human immunodeficiency virus recombinant DNA and recombinant vaccinia virus Tian Tan prime-boost immunization.
abstract: This study assessed and compared the immunogenicity of various immunization strategies in mice using combinations of recombinant DNA (pCCMp24) and recombinant attenuated vaccinia virus Tian Tan (rddVTT-CCMp24). Intramuscular immunization was performed on days 0 (prime) and 21 (boost). The immunogenicity of the vaccine schedules was determined by measuring human immunodeficiency virus (HIV)-specific binding antibody levels and cytokine (interleukin-2 and interleukin-4) concentrations in peripheral blood, analyzing lymphocyte proliferation capacity against HIV epitopes and CD4(+)/CD8(+) cell ratio, and monitoring interferon-gamma levels at different times post-immunization. The results showed that pCCMp24, rddVTT-CCMp24 and their prime-boost immunization induced humoral and cellular immune responses. The pCCMp24/rddVTT-CCMp24 immunization strategy increased CD8(+) T cells and induced more IFN-γ-secreting cells compared with single-shot rDNA. The prime-boost immunization strategy also induced the generation of cellular immunological memory to HIV epitope peptides. These results demonstrated that prime-boost immunization with rDNA and rddVTT-CCMp24 had a tendency to induce greater cellular immune response than single-shot vaccinations, especially IFN-γ response, providing a basis for further studies.
authors: College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China.
authors: Cunxia
authors: C
authors: Liu
authors: Shouwen
authors: S
authors: Du
authors: Chang
authors: C
authors: Li
authors: Yuhang
authors: Y
authors: Wang
authors: Maopeng
authors: M
authors: Wang
authors: Yi
authors: Y
authors: Li
authors: Ronglan
authors: R
authors: Yin
authors: Xiao
authors: X
authors: Li
authors: Dayong
authors: D
authors: Ren
authors: Yanqing
authors: Y
authors: Qin
authors: Jingqiang
authors: J
authors: Ren
authors: Ningyi
authors: N
authors: Jin
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology*; D016516:CD4-CD8 Ratio; D049109:Cell Proliferation; D004274:DNA, Recombinant / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D018984:Epitopes, T-Lymphocyte; D005260:Female; D006678:HIV / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007114:Immunization / Q000379:methods*; D007117:Immunization, Secondary / Q000379:methods; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D007376:Interleukin-2 / Q000097:blood / Q000276:immunology / Q000378:metabolism; D015847:Interleukin-4 / Q000097:blood / Q000276:immunology / Q000378:metabolism; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D011995:Recombination, Genetic; D014616:Vaccinia virus / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D004274:DNA, Recombinant; D018984:Epitopes, T-Lymphocyte; D007376:Interleukin-2; D014765:Viral Vaccines; D015847:Interleukin-4; D007371:Interferon-gamma