title: Cerebral low-molecular metabolites influenced by intestinal microbiota: a pilot study.
abstract: Recent studies suggest that intestinal microbiota influences gut-brain communication. In this study, we aimed to clarify the influence of intestinal microbiota on cerebral metabolism. We analyzed the cerebral metabolome of germ-free (GF) mice and Ex-GF mice, which were inoculated with suspension of feces obtained from specific pathogen-free mice, using capillary electrophoresis with time-of-flight mass spectrometry (CE-TOFMS). CE-TOFMS identified 196 metabolites from the cerebral metabolome in both GF and Ex-GF mice. The concentrations of 38 metabolites differed significantly (p < 0.05) between GF and Ex-GF mice. Approximately 10 of these metabolites are known to be involved in brain function, whilst the functions of the remainder are unclear. Furthermore, we observed a novel association between cerebral glycolytic metabolism and intestinal microbiota. Our work shows that cerebral metabolites are influenced by normal intestinal microbiota through the microbiota-gut-brain axis, and indicates that normal intestinal microbiota closely connected with brain health and disease, development, attenuation, learning, memory, and behavior.
authors: Dairy Science and Technology Institute, Kyodo Milk Industry Co. Ltd. Hinode-machi, Tokyo, Japan.
authors: Mitsuharu
authors: M
authors: Matsumoto
authors: Ryoko
authors: R
authors: Kibe
authors: Takushi
authors: T
authors: Ooga
authors: Yuji
authors: Y
authors: Aiba
authors: Emiko
authors: E
authors: Sawaki
authors: Yasuhiro
authors: Y
authors: Koga
authors: Yoshimi
authors: Y
authors: Benno
keywords: cerebrum; gut-brain axis; intestinal microbiota; metabolome; neurotransmitter
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