title: Saccharothrix saharensis sp. nov., an actinomycete isolated from Algerian Saharan soil.
abstract: The taxonomic position of a novel actinomycete, strain SA152(T), isolated from a sample of Algerian Saharan soil, was determined using a polyphasic taxonomic approach. The strain produced abundant aerial mycelium and fragmented substrate mycelium on most media tested. Chemotaxonomically and phylogenetically, the strain was related to the members of the genus Saccharothrix. Results of 16S rRNA gene sequence comparison revealed that strain SA152(T) shared the highest degree of 16S rRNA gene sequence similarity with Saccharothrix xinjiangensis NBRC 101911(T) (99.3 %) and Saccharothrix texasensis NRRL B-16134(T) (98.9 %). However, DNA-DNA hybridization studies showed only 16.2 % relatedness with S. xinjiangensis DSM 44896(T) and 33.9 % relatedness with S. texasensis DSM 44231(T). Based upon genotypic and phenotypic differences from other members of the genus, a novel species, Saccharothrix saharensis sp. nov., is proposed, with SA152(T) ( = DSM 45456(T) = CCUG 60213(T)) as the type strain.
authors: Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algeria.
authors: Dalila
authors: D
authors: Boubetra
authors: Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algeria.
authors: Abdelghani
authors: A
authors: Zitouni
authors: Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algeria.
authors: Noureddine
authors: N
authors: Bouras
authors: Université de Toulouse, INPT-ENSAT, Laboratoire de Génie Chimique, UMR 5503 (CNRS/INPT/UPS), 1 Avenue de l'Agrobiopole BP 32607 Auzeville Tolosane, 31326 Castanet-Tolosan, France.
authors: Florence
authors: F
authors: Mathieu
authors: Université de Toulouse, INPT-ENSAT, Laboratoire de Génie Chimique, UMR 5503 (CNRS/INPT/UPS), 1 Avenue de l'Agrobiopole BP 32607 Auzeville Tolosane, 31326 Castanet-Tolosan, France.
authors: Ahmed
authors: A
authors: Lebrihi
authors: DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
authors: Peter
authors: P
authors: Schumann
authors: DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
authors: Cathrin
authors: C
authors: Spröer
authors: DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
authors: Hans-Peter
authors: HP
authors: Klenk
authors: Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algeria.
authors: Nasserdine
authors: N
authors: Sabaou
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D000462:Algeria; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005838:Genotype; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010714:Phosphatidylethanolamines / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C073355:menaquinone 9; D003960:Diaminopimelic Acid