title: Ecdysone triggered PGRP-LC expression controls Drosophila innate immunity.
abstract: Throughout the animal kingdom, steroid hormones have been implicated in the defense against microbial infection, but how these systemic signals control immunity is unclear. Here, we show that the steroid hormone ecdysone controls the expression of the pattern recognition receptor PGRP-LC in Drosophila, thereby tightly regulating innate immune recognition and defense against bacterial infection. We identify a group of steroid-regulated transcription factors as well as two GATA transcription factors that act as repressors and activators of the immune response and are required for the proper hormonal control of PGRP-LC expression. Together, our results demonstrate that Drosophila use complex mechanisms to modulate innate immune responses, and identify a transcriptional hierarchy that integrates steroid signalling and immunity in animals.
authors: Department of Medicine, Division of Infectious Disease, University of Massachusetts Medical School, Worcester, MA, USA.
authors: Florentina
authors: F
authors: Rus
authors: Thomas
authors: T
authors: Flatt
authors: Mei
authors: M
authors: Tong
authors: Kamna
authors: K
authors: Aggarwal
authors: Kendi
authors: K
authors: Okuda
authors: Anni
authors: A
authors: Kleino
authors: Elisabeth
authors: E
authors: Yates
authors: Marc
authors: M
authors: Tatar
authors: Neal
authors: N
authors: Silverman
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D004330:Drosophila / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D004440:Ecdysone / Q000378:metabolism*; D016972:Enterobacter cloacae / Q000502:physiology; D050980:GATA Transcription Factors / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D007113:Immunity, Innate; D053208:Kaplan-Meier Estimate; D008958:Models, Molecular; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D016973:Pectobacterium carotovorum / Q000502:physiology; D034622:RNA Interference; D011987:Receptors, Steroid / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: C102117:Br protein, Drosophila; D002352:Carrier Proteins; D029721:Drosophila Proteins; D050980:GATA Transcription Factors; D011987:Receptors, Steroid; D014157:Transcription Factors; C055469:ecdysone receptor; C118897:peptidoglycan recognition protein; C109259:pnr protein, Drosophila; C103965:srp protein, Drosophila; D004440:Ecdysone
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