title: Identification of MV-generated ROS responsive EST clones in floral buds of Litchi chinensis Sonn.
abstract: A suppression subtractive hybridization library was constructed using inflorescence primordia of 'Nuomici' litchi to identify EST clones responsive to MV-generated ROS. 93 ESTs could be aligned as unique gene sequences in the inflorescence primordia of litchi. Litchi is an evergreen woody tree widely cultivated in subtropical and tropical regions. However, defective flowering is a pending problem of litchi production. Our previous study indicated that reactive oxygen species (ROS) induced by methyl viologen dichloride hydrate (MV) promotes flowering in litchi. In the present study, a suppression subtractive hybridization (SSH) library was constructed using inflorescence primordia of 'Nuomici' with the aim to find out ROS responsive clones during floral differentiation. 1856 Expressed sequence tag (EST) clones were randomly selected. Clones carrying single exogenous fragments were screened by reverse northern analysis to identify those responsive to MV-generated ROS. A total of 783 differentially expressed EST clones were identified as MV responsive cDNA and were subjected to sequencing. Among them, 26 clones were represented more than three times. 783 clones were aligned to 93 unique gene sequences. The unique genes were classified into 9 categories. 16 % of them were involved in transport facilitation, 11 % in transcription regulation, 4 % in stress response, 9 % in carbohydrate metabolism, 1 % in secondary metabolism, 14 % in intracellular signaling, and 25 % in other metabolism, while 9 % were genes with unknown functions and 11 % were genes with no match in the database.
authors: College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
authors: Wei-Wei
authors: WW
authors: Liu
authors: Hye-Ji
authors: HJ
authors: Kim
authors: Hou-Bin
authors: HB
authors: Chen
authors: Xing-Yu
authors: XY
authors: Lu
authors: Bi-Yan
authors: BY
authors: Zhou
mesh_terms: D020224:Expressed Sequence Tags; D035264:Flowers / Q000235:genetics* / Q000502:physiology; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D032125:Litchi / Q000235:genetics* / Q000502:physiology; D010269:Paraquat / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species; D010269:Paraquat
references: J Mol Biol. 1995 Aug 25;251(4):533-49
references: 7658471
references: Development. 2005 Oct;132(20):4521-31
references: 16192309
references: Plant J. 2002 Dec;32(5):669-83
references: 12472684
references: Plant Cell Environ. 2006 Jun;29(6):1049-60
references: 17080932
references: J Exp Bot. 2008;59(12):3215-28
references: 18653697
references: Annu Rev Genet. 2006;40:77-105
references: 17094738
references: Plant Cell. 1993 Dec;5(12):1711-23
references: 8305869
references: Curr Opin Plant Biol. 2001 Jun;4(3):247-53
references: 11312136
references: Plant Mol Biol. 2011 Jan;75(1-2):35-51
references: 20972607
references: Nature. 2005 Jan 6;433(7021):39-44
references: 15635403
references: Curr Opin Plant Biol. 2009 Feb;12(1):75-80
references: 18938104
references: Dev Cell. 2012 May 15;22(5):1030-40
references: 22595674
references: Plant Sci. 2008 Mar 1;174(3):366-373
references: 19568326
references: Cell Mol Life Sci. 2000 May;57(5):779-95
references: 10892343
references: Proc Natl Acad Sci U S A. 1996 Jun 11;93(12):6025-30
references: 8650213
references: Science. 2006 May 12;312(5775):883
references: 16601151
references: Nature. 1989 Mar 30;338(6214):394-401
references: 2538753
references: Curr Opin Plant Biol. 1999 Oct;2(5):410-8
references: 10508760
references: Endeavour. 1971 Sep;30(111):130-5
references: 4110469
references: Genes Dev. 2006 Oct 15;20(20):2902-11
references: 17043314
references: Biochem J. 2001 Feb 1;353(Pt 3):417-39
references: 11171037
references: Development. 2004 Aug;131(15):3615-26
references: 15229176
references: N Biotechnol. 2010 Sep 30;27(4):289-99
references: 20123048
references: Curr Opin Plant Biol. 2005 Feb;8(1):93-102
references: 15653406
references: J Plant Physiol. 2009 Mar 15;166(5):548-53
references: 18771815
references: Plant Mol Biol. 2011 May;76(1-2):187-204
references: 21533840
references: Gene. 2012 Jan 15;492(1):220-8
references: 22056698