title: Gamma-tocotrienol modulated gene expression in senescent human diploid fibroblasts as revealed by microarray analysis.
abstract: The effect of γ -tocotrienol, a vitamin E isomer, in modulating gene expression in cellular aging of human diploid fibroblasts was studied. Senescent cells at passage 30 were incubated with 70  μ M of γ -tocotrienol for 24 h. Gene expression patterns were evaluated using Sentrix HumanRef-8 Expression BeadChip from Illumina, analysed using GeneSpring GX10 software, and validated using quantitative RT-PCR. A total of 100 genes were differentially expressed (P < 0.001) by at least 1.5 fold in response to γ -tocotrienol treatment. Amongst the genes were IRAK3, SelS, HSPA5, HERPUD1, DNAJB9, SEPR1, C18orf55, ARF4, RINT1, NXT1, CADPS2, COG6, and GLRX5. Significant gene list was further analysed by Gene Set Enrichment Analysis (GSEA), and the Normalized Enrichment Score (NES) showed that biological processes such as inflammation, protein transport, apoptosis, and cell redox homeostasis were modulated in senescent fibroblasts treated with γ -tocotrienol. These findings revealed that γ -tocotrienol may prevent cellular aging of human diploid fibroblasts by modulating gene expression.
authors: Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia. suzanamakpol@yahoo.com
authors: Suzana
authors: S
authors: Makpol
authors: Azalina
authors: A
authors: Zainuddin
authors: Kien Hui
authors: KH
authors: Chua
authors: Yasmin Anum
authors: YA
authors: Mohd Yusof
authors: Wan Zurinah Wan
authors: WZ
authors: Ngah
mesh_terms: D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects; D002839:Chromans / Q000494:pharmacology*; D016000:Cluster Analysis; D004171:Diploidy; D000091342:Endoplasmic Reticulum Chaperone BiP; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D053263:Gene Regulatory Networks; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D060888:Real-Time Polymerase Chain Reaction; D014810:Vitamin E / Q000031:analogs & derivatives* / Q000494:pharmacology
chemical_list: D002839:Chromans; D000091342:Endoplasmic Reticulum Chaperone BiP; C000717247:HSPA5 protein, human; D014810:Vitamin E; C013649:plastochromanol 8
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