title: Grafts of porcine small intestinal submucosa seeded with cultured homologous smooth muscle cells for bladder repair in dogs.
abstract: BACKGROUND
  Due to numerous complications associated to gastrointestinal augmented cystoplasty, this study aimed to analyze the anatomic repair of the bladder of 10 female dogs using grafts of porcine small intestinal submucosa (SIS) seeded with cultured homologous smooth muscle cells, and compare them with the acellular SIS grafts.
  
  
  RESULTS
  We assessed the possible side effects and complications of each type of graft by clinical examination, abdominal ultrasound and laboratory findings. Anatomic repair of neoformed bladder was assessed by histological staining for H/E and Masson's Trichrome, analyzed with a Nikon Photomicroscope connected to the system of image analysis Image J.
  
  
  CONCLUSIONS
  We propose that SIS associated to homologous smooth cells can improve the quality of tissue repair, and consequently decrease the potential complications inherent to acellular SIS.
authors: Department of Veterinary Surgery and Anesthesiology, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Rubião Júnior, S/N, Botucatu, ZIP COD 18,618-970, Brazil. vjvrossetto@hotmail.com
authors: Victor J V
authors: VJ
authors: Rossetto
authors: Lígia S L S
authors: LS
authors: da Mota
authors: Noeme S
authors: NS
authors: Rocha
authors: Hélio A
authors: HA
authors: Miot
authors: Fabrizio
authors: F
authors: Grandi
authors: Cláudia V S
authors: CV
authors: Brandão
mesh_terms: D000818:Animals; D017690:Cell Transplantation / Q000379:methods / Q000662:veterinary*; D018920:Coculture Techniques; D003094:Collagen / Q000502:physiology; D004285:Dogs; D005260:Female; D007421:Intestine, Small; D032389:Myocytes, Smooth Muscle; D013552:Swine; D001743:Urinary Bladder / Q000473:pathology*
chemical_list: D003094:Collagen
references: J Vet Intern Med. 2003 Sep-Oct;17(5):622-31
references: 14529127
references: BJU Int. 2004 Oct;94(6):859-66
references: 15476523
references: Urology. 1998 Feb;51(2):221-5
references: 9495701
references: J Endourol. 2000 Feb;14(1):59-62
references: 10735574
references: BJU Int. 2006 Nov;98(5):1100-5
references: 17034611
references: Med Hypotheses. 2008;70(2):294-7
references: 17669597
references: J Endourol. 1993 Oct;7(5):423-5
references: 8298626
references: J Endourol. 2000 Feb;14(1):49-57
references: 10735573
references: J Urol. 1996 Jun;155(6):2098-104
references: 8618344
references: BJU Int. 2005 Nov;96(7):1120-5
references: 16225540
references: Ann Urol (Paris). 2004 Dec;38(6):298-310
references: 15651484
references: Annu Rev Biomed Eng. 1999;1:103-27
references: 11701484
references: J Urol. 2008 May;179(5):2035-41
references: 18355869
references: Methods. 2009 Feb;47(2):109-15
references: 18952175
references: Biomaterials. 2006 Mar;27(7):1054-60
references: 16174527
references: J Bone Miner Metab. 2008;26(2):115-22
references: 18301966
references: J Urol. 1999 Sep;162(3 Pt 2):1096-100
references: 10458439