title: A refined model of the prototypical Salmonella SPI-1 T3SS basal body reveals the molecular basis for its assembly.
abstract: The T3SS injectisome is a syringe-shaped macromolecular assembly found in pathogenic Gram-negative bacteria that allows for the direct delivery of virulence effectors into host cells. It is composed of a "basal body", a lock-nut structure spanning both bacterial membranes, and a "needle" that protrudes away from the bacterial surface. A hollow channel spans throughout the apparatus, permitting the translocation of effector proteins from the bacterial cytosol to the host plasma membrane. The basal body is composed largely of three membrane-embedded proteins that form oligomerized concentric rings. Here, we report the crystal structures of three domains of the prototypical Salmonella SPI-1 basal body, and use a new approach incorporating symmetric flexible backbone docking and EM data to produce a model for their oligomeric assembly. The obtained models, validated by biochemical and in vivo assays, reveal the molecular details of the interactions driving basal body assembly, and notably demonstrate a conserved oligomerization mechanism.
authors: Department of Biochemistry and Molecular Biology, and Centre for Blood Research, University of British Columbia, Vancouver, British Columbia, Canada.
authors: Julien R C
authors: JR
authors: Bergeron
authors: Liam J
authors: LJ
authors: Worrall
authors: Nikolaos G
authors: NG
authors: Sgourakis
authors: Frank
authors: F
authors: DiMaio
authors: Richard A
authors: RA
authors: Pfuetzner
authors: Heather B
authors: HB
authors: Felise
authors: Marija
authors: M
authors: Vuckovic
authors: Angel C
authors: AC
authors: Yu
authors: Samuel I
authors: SI
authors: Miller
authors: David
authors: D
authors: Baker
authors: Natalie C J
authors: NC
authors: Strynadka
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D058947:Bacterial Secretion Systems; D002462:Cell Membrane / Q000378:metabolism*; D018360:Crystallography, X-Ray; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D017434:Protein Structure, Tertiary; D012486:Salmonella typhimurium / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D058947:Bacterial Secretion Systems; D008565:Membrane Proteins
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