title: CENP-A confers a reduction in height on octameric nucleosomes.
abstract: Nucleosomes with histone H3 replaced by CENP-A direct kinetochore assembly. CENP-A nucleosomes from human and Drosophila have been reported to have reduced heights as compared to canonical octameric H3 nucleosomes, thus suggesting a unique tetrameric hemisomal composition. We demonstrate that octameric CENP-A nucleosomes assembled in vitro exhibit reduced heights, indicating that they are physically distinct from H3 nucleosomes and negating the need to invoke the presence of hemisomes.
authors: Wellcome Trust Centre for Cell Biology and Institute of Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, Scotland, UK.
authors: Matthew D D
authors: MD
authors: Miell
authors: Colin J
authors: CJ
authors: Fuller
authors: Annika
authors: A
authors: Guse
authors: Helena M
authors: HM
authors: Barysz
authors: Andrew
authors: A
authors: Downes
authors: Tom
authors: T
authors: Owen-Hughes
authors: Juri
authors: J
authors: Rappsilber
authors: Aaron F
authors: AF
authors: Straight
authors: Robin C
authors: RC
authors: Allshire
mesh_terms: D001324:Autoantigens / Q000378:metabolism*; D000076247:Centromere Protein A; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D006801:Humans; D018625:Microscopy, Atomic Force; D009707:Nucleosomes / Q000378:metabolism* / Q000648:ultrastructure*
chemical_list: D001324:Autoantigens; C000619700:CENPA protein, human; D000076247:Centromere Protein A; D002868:Chromosomal Proteins, Non-Histone; D009707:Nucleosomes
references: Mol Cell. 2009 Sep 24;35(6):794-805
references: 19782029
references: Methods. 2009 Mar;47(3):206-13
references: 18835446
references: PLoS Biol. 2007 Aug;5(8):e218
references: 17676993
references: Eur Biophys J. 2007 Dec;37(1):81-93
references: 17503032
references: Mol Cell. 2012 Jan 27;45(2):263-9
references: 22209075
references: Am J Hum Genet. 1993 Jun;52(6):1153-63
references: 7684888
references: Proc Natl Acad Sci U S A. 2011 Oct 4;108(40):16588-93
references: 21949362
references: Nat Protoc. 2006;1(6):2856-60
references: 17406544
references: J Mol Biol. 2007 Jul 13;370(3):555-73
references: 17524417
references: Nature. 1997 Sep 18;389(6648):251-60
references: 9305837
references: Anal Chem. 2003 Feb 1;75(3):663-70
references: 12585499
references: Proc Natl Acad Sci U S A. 2007 Mar 20;104(12):5008-13
references: 17360341
references: J Mol Biol. 2005 Feb 4;345(5):957-68
references: 15644197
references: Proc Natl Acad Sci U S A. 2010 Nov 23;107(47):20317-22
references: 21059934
references: Methods Mol Biol. 1999;119:1-16
references: 10804500
references: Biophys J. 1998 Jun;74(6):2823-9
references: 9635736
references: Nature. 2004 Jul 29;430(6999):578-82
references: 15282608
references: Cell. 2012 Jul 20;150(2):317-26
references: 22817894