title: Respiratory syncytial virus: co-infection and paediatric lower respiratory tract infections.
abstract: Comprehensive population-based data on the role of respiratory viruses in the development of lower respiratory tract infections (LRTIs) remain unclear. We investigated the incidence and effect of single and multiple infections with respiratory viruses on the risk of LRTIs in Vietnam. Population-based prospective surveillance and a case-control study of hospitalised paediatric patients with acute respiratory infection (ARI) were conducted from April 2007 through to March 2010. Healthy controls were randomly recruited from the same community. Nasopharyngeal samples were collected and tested for 13 respiratory viruses using multiplex PCRs. 1992 hospitalised ARI episodes, including 397 (19.9%) with LRTIs, were enrolled. Incidence of hospitalised LRTIs among children aged <24 months was 2171.9 per 100 000 (95% CI 1947.9-2419.7). The majority of ARI cases (60.9%) were positive for at least one virus. Human rhinovirus (24.2%), respiratory syncytial virus (20.1%) and influenza A virus (12.0%) were the most common and 9.5% had multiple-viral infections. Respiratory syncytial virus and human metapneumovirus infections independently increased the risk of LRTIs. Respiratory syncytial virus further increased the risk, when co-infected with human rhinovirus, human metapneumovirus and parainfluenza virus-3 but not with influenza A virus. The case-control analysis revealed that respiratory syncytial virus and influenza A virus increased the risk of ARI hospitalisation but not human rhinovirus. Respiratory syncytial virus is the leading pathogen associated with risk of ARI hospitalisation and LRTIs in Vietnam.
authors: Department of Clinical Medicine, Institute of Tropical Medicine, Nagasaki University 1-12-4, Sakamoto, Nagasaki, 852-8523, Japan.
authors: Lay-Myint
authors: LM
authors: Yoshida
authors: Motoi
authors: M
authors: Suzuki
authors: Hien Anh
authors: HA
authors: Nguyen
authors: Minh Nhat
authors: MN
authors: Le
authors: Thiem
authors: T
authors: Dinh Vu
authors: Hiroshi
authors: H
authors: Yoshino
authors: Wolf-Peter
authors: WP
authors: Schmidt
authors: Thi Thuy Ai
authors: TT
authors: Nguyen
authors: Huu Tho
authors: HT
authors: Le
authors: Konosuke
authors: K
authors: Morimoto
authors: Hiroyuki
authors: H
authors: Moriuchi
authors: Duc Anh
authors: DA
authors: Dang
authors: Koya
authors: K
authors: Ariyoshi
mesh_terms: D000465:Algorithms; D016022:Case-Control Studies; D002675:Child, Preschool; D060085:Coinfection; D005260:Female; D006760:Hospitalization; D006801:Humans; D015994:Incidence; D007223:Infant; D008297:Male; D009297:Nasal Mucosa / Q000378:metabolism; D016133:Polymerase Chain Reaction; D011159:Population Surveillance; D011446:Prospective Studies; D018357:Respiratory Syncytial Virus Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology*; D012136:Respiratory Syncytial Viruses; D012141:Respiratory Tract Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology*; D014744:Vietnam / Q000453:epidemiology