title: Redox balance via lactate dehydrogenase is important for multiple stress resistance and virulence in Enterococcus faecalis.
abstract: Enterococcus faecalis is a highly stress resistant opportunistic pathogen. The intrinsic ruggedness of this bacterium is supposed to be the basis of its capacity to colonize the hostile environments of hospitals and to cause several kinds of infections. We show in this work that general resistance to very different environmental stresses depends on the ability of E. faecalis to maintain redox balance via lactate dehydrogenase (LDH). Furthermore, LDH-deficient mutants are less successful than the wild type at colonizing host organs in a murine model of systemic infection. Taken together, our results, as well as those previously published for Staphylococcus aureus (A. R. Richardson, S. J. Libby, and F. C. Fang, Science 319:1672-1676, 2008), identify LDH as an attractive drug target. These drugs may have additional applications, as in the fight against glycopeptide antibiotic-resistant bacteria and even cancer.
authors: Université de Caen Basse-Normandie, EA4655 U2RM-Stress and Virulence, Caen, France.
authors: Nosheen Fatima
authors: NF
authors: Rana
authors: Nicolas
authors: N
authors: Sauvageot
authors: Jean-Marie
authors: JM
authors: Laplace
authors: Yinyin
authors: Y
authors: Bao
authors: Ingolf
authors: I
authors: Nes
authors: Alain
authors: A
authors: Rincé
authors: Brunella
authors: B
authors: Posteraro
authors: Maurizio
authors: M
authors: Sanguinetti
authors: Axel
authors: A
authors: Hartke
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism; D013293:Enterococcus faecalis / Q000378:metabolism* / Q000472:pathogenicity*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism*; D051379:Mice; D010084:Oxidation-Reduction; D013312:Stress, Physiological / Q000502:physiology*; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D007770:L-Lactate Dehydrogenase
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