title: A high-speed magnetic tweezer beyond 10,000 frames per second.
abstract: The magnetic tweezer is a single-molecule instrument that can apply a constant force to a biomolecule over a range of extensions, and is therefore an ideal tool to study biomolecules and their interactions. However, the video-based tracking inherent to most magnetic single-molecule instruments has traditionally limited the instrumental resolution to a few nanometers, above the length scale of single DNA base-pairs. Here we have introduced superluminescent diode illumination and high-speed camera detection to the magnetic tweezer, with graphics processing unit-accelerated particle tracking for high-speed analysis of video files. We have demonstrated the ability of the high-speed magnetic tweezer to resolve particle position to within 1 Å at 100 Hz, and to measure the extension of a 1566 bp DNA with 1 nm precision at 100 Hz in the presence of thermal noise.
authors: Materials Department, University of California Santa Barbara, Santa Barbara, California 93106, USA.
authors: Bob M
authors: BM
authors: Lansdorp
authors: Shawn J
authors: SJ
authors: Tabrizi
authors: Andrew
authors: A
authors: Dittmore
authors: Omar A
authors: OA
authors: Saleh
mesh_terms: D004247:DNA / Q000737:chemistry*; D008280:Magnetics / Q000295:instrumentation* / Q000379:methods*; D008962:Models, Theoretical; D014741:Video Recording / Q000295:instrumentation / Q000379:methods
chemical_list: D004247:DNA