title: Multiscale analysis of collagen microstructure with generalized image correlation spectroscopy and the detection of tissue prestress.
abstract: Prestress in tissue is currently detected through destructive methods which obviate both in vivo and longitudinal assessment. We hypothesized that prestress could be detected and quantified by analyzing the microstructure of the extracellular matrix at different spatial scales using non-invasive and non-destructive optical imaging. A simple model of tissue prestress was created using fibroblast-mediated contraction of collagen gels around a central mandrel. Using a quantitative, multiscale, image processing technique, termed generalized image correlation spectroscopy (GICS) of second harmonic images, collagen fiber number and alignment at three different length scales characteristic of the collagen fibril, collagen fiber, and cell were analyzed. GICS fiber alignment (σ(maj/min)) was significantly different across load state, level of prestress, and length scale. The largest fiber ratio, and thus highest alignment, was seen in prestressed, externally loaded gels at a length scale equivalent to the size of the fibroblast cells. Alignment at both fiber and cell scale correlated with prestress in this model. We conclude that GICS of second harmonic images of collagen can predict prestress, and that microstructural organization at the collagen fiber and cell scale are the primary determinants of prestress in cellularized collagen gels.
authors: Department of Biomedical Engineering, University of California, Irvine, CA, USA.
authors: Claire
authors: C
authors: Robertson
authors: Kenji
authors: K
authors: Ikemura
authors: Tatiana B
authors: TB
authors: Krasieva
authors: Steven C
authors: SC
authors: George
mesh_terms: D000818:Animals; D002460:Cell Line; D003094:Collagen / Q000737:chemistry / Q000648:ultrastructure*; D005109:Extracellular Matrix / Q000737:chemistry / Q000648:ultrastructure*; D005347:Fibroblasts / Q000737:chemistry / Q000166:cytology; D006801:Humans; D036641:Microscopy, Fluorescence, Multiphoton; D061848:Optical Imaging; D051381:Rats; D013314:Stress, Mechanical
chemical_list: D003094:Collagen
references: J Biomech Eng. 2009 Aug;131(8):081006
references: 19604018
references: PLoS One. 2009 Jun 16;4(6):e5902
references: 19529768
references: Cell Mol Bioeng. 2009 Mar 1;2(1):39-48
references: 20582230
references: J Biomech Eng. 2007 Oct;129(5):642-50
references: 17887889
references: Biophys J. 1993 Sep;65(3):1135-46
references: 8241393
references: Biophys J. 2001 Nov;81(5):2954-63
references: 11606305
references: J Biomech. 2010 Sep 17;43(13):2637-40
references: 20494363
references: Acta Biomater. 2012 Nov;8(11):4031-42
references: 22828381
references: J Biomed Opt. 2012 Aug;17(8):080801-1
references: 23224160
references: Am J Physiol Heart Circ Physiol. 2007 Sep;293(3):H1987-96
references: 17604326
references: Ann Biomed Eng. 2013 Mar;41(3):547-61
references: 23180029
references: Microsc Res Tech. 2008 Apr;71(4):279-87
references: 18072283
references: Science. 2005 Nov 18;310(5751):1139-43
references: 16293750
references: J Biomech Eng. 1989 Nov;111(4):325-35
references: 2486372
references: J Biomech. 2003 Jan;36(1):97-102
references: 12485643
references: Nat Biotechnol. 2003 Nov;21(11):1369-77
references: 14595365
references: Biophys J. 2001 Nov;81(5):2964-71
references: 11606306
references: J Biomech Eng. 2006 Dec;128(6):890-8
references: 17154691
references: Tissue Eng Part C Methods. 2013 Oct;19(10):802-9
references: 23469868
references: Ann Biomed Eng. 2002 Nov-Dec;30(10):1221-33
references: 12540198
references: J Theor Biol. 2012 Jun 21;303:93-7
references: 22763133
references: J Cell Sci. 2010 Feb 1;123(Pt 3):297-308
references: 20130138
references: Arterioscler Thromb Vasc Biol. 1997 Oct;17(10):2209-17
references: 9351391
references: Biophys J. 2008 Mar 15;94(6):2361-73
references: 18065452
references: Circ Res. 2006 Jan 6;98(1):25-35
references: 16397155
references: Biomech Model Mechanobiol. 2009 Dec;8(6):431-46
references: 19123012
references: Nat Rev Mol Cell Biol. 2002 May;3(5):349-63
references: 11988769
references: J Biomech Eng. 2011 Sep;133(9):091011
references: 22010746
references: Ann Biomed Eng. 2005 Nov;33(11):1469-90
references: 16341917
references: Ann Biomed Eng. 2001 Jun;29(6):467-75
references: 11459340
references: Acta Biomater. 2010 Dec;6(12):4657-65
references: 20620246
references: Biophys J. 2007 Mar 15;92(6):2212-22
references: 17172303
references: Ann Biomed Eng. 2010 May;38(5):1741-51
references: 20232150
references: Comput Methods Appl Mech Eng. 2009 Sep 15;198(45-46):3514-3523
references: 20161129