title: An in vitro autogene.
abstract: Recent technological advances have allowed development of increasingly complex systems for in vitro evolution. Here, we describe an in vitro autogene composed of a self-amplifying T7 RNA polymerase system. Functional autogene templates in cell-free lysate produce T7 RNA polymerase, which amplifies the autogene genetic information through a positive feedback architecture. Compartmentalization of individual templates within a water-in-oil emulsion links genotype and phenotype, allowing evolution.
authors: Department of Chemistry and Biochemistry, Institute for Cell and Molecular Biology, Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, TX 78712, USA.
authors: Eric A
authors: EA
authors: Davidson
authors: Adam J
authors: AJ
authors: Meyer
authors: Jared W
authors: JW
authors: Ellefson
authors: Matthew
authors: M
authors: Levy
authors: Andrew D
authors: AD
authors: Ellington
mesh_terms: D017123:Bacteriophage T7 / Q000201:enzymology / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000235:genetics*; D019020:Directed Molecular Evolution; D025461:Feedback, Physiological; D005814:Genes, Viral; D009690:Nucleic Acid Conformation; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D058615:Synthetic Biology; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D012367:RNA, Viral; D014764:Viral Proteins; C070852:RNA polymerase SP6; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases