title: Anti-inflammatory secondary metabolites from the leaves of Rosa laevigata.
abstract: Bioassay-guided fractionation of a n-BuOH-soluble extract of the leaves of Rosa laevigata led to the isolation of three new 19-oxo-18,19-seco-ur-sane-type triterpenoids, laevigins A-C (1-3), a new oleanane-type triterpenoid saponin, laevigin D (4), a new geranylmethylbenzoate, 5-[(2″E,6″S)-6″,7″-dihydroxy-3″,7″-dimethyl-2″-octen-1″-yl]-2-(β-D-glucopyranosyloxy)-methyl benzoate (5), together with 9 known compounds (6-14). Their structures were elucidated by spectroscopic and chemical methods. Compounds 4, 9, 11, and 12 significantly suppressed the LPS-stimulated NF-κB transcriptional activity and the release of TNFα, IL-1β, IL-6, and IL-10 in mouse RAW 264.7 macrophages. The compound 12 exhibited moderate inhibition on NF-κB transcriptional activity with an IC50 value of 23.21 μM. The IC50 values of compound 12 were measured as 14.32, 8.53, 8.04, and 10.38 μM for the inhibitory activity on TNFα-release, IL-1β-release, IL-6-release, and IL-10-release, respectively.
authors: Department of Pharmacy, Renji Hospital, Affiliated to School of Medicine, Shanghai Jiaotong University, Dongfang Road 1630, Shanghai 200127, People's Republic of China.
authors: Min
authors: M
authors: Yan
authors: Yan
authors: Y
authors: Zhu
authors: Hong-Jun
authors: HJ
authors: Zhang
authors: Wei-Hua
authors: WH
authors: Jiao
authors: Bing-Nan
authors: BN
authors: Han
authors: Zhao-Xi
authors: ZX
authors: Liu
authors: Feng
authors: F
authors: Qiu
authors: Wan-Sheng
authors: WS
authors: Chen
authors: Hou-Wen
authors: HW
authors: Lin
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D002460:Cell Line; D016753:Interleukin-10 / Q000096:biosynthesis / Q000378:metabolism; D053583:Interleukin-1beta / Q000096:biosynthesis / Q000378:metabolism; D015850:Interleukin-6 / Q000096:biosynthesis / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D016328:NF-kappa B / Q000235:genetics / Q000276:immunology; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D027826:Rosa / Q000737:chemistry*; D013729:Terpenes / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D015533:Transcriptional Activation / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000378:metabolism
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; C556062:IL10 protein, mouse; D053583:Interleukin-1beta; D015850:Interleukin-6; D008070:Lipopolysaccharides; D016328:NF-kappa B; D010936:Plant Extracts; D013729:Terpenes; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10