title: Isolation of multipotent nestin-expressing stem cells derived from the epidermis of elderly humans and TAT-VHL peptide-mediated neuronal differentiation of these cells.
abstract: A specialized population of cells residing in the hair follicle is quiescent but shows pluripotency for differentiating into epithelial-mesenchymal lineage cells. Therefore, such cells are hoped to be useful as implantable donor cells for regenerative therapy. Recently, it was reported that intracellular delivery of TAT-VHL peptide induces neuronal differentiation of skin-derived precursors. In the present study, we successfully isolated multipotent stem cells derived from the epidermis of elderly humans, characterized these cells as being capable of sphere formation and strong expression of nestin, fibronectin, and CD34 but not of keratin 15, and identified the niche of these cells as being the outer root sheath of the hair follicles. In addition, we showed that TAT-VHL peptide induced their neuronal differentiation in vitro, and confirmed by fluorescence immunohistochemistry the neuronal differentiation of such peptide-treated cells implanted into rodent brains. These multipotent nestin-expressing stem cells derived from human epidermis are easily accessible and should be useful as donor cells for neuronal regenerative cell therapy.
authors: Department of Neurosurgery, Yokohama City University, Yokohama 236-0004, Japan. hiroshikannomd@nifty.com.
authors: Hiroshi
authors: H
authors: Kanno
authors: Atsuhiko
authors: A
authors: Kubo
authors: Tetsuya
authors: T
authors: Yoshizumi
authors: Taro
authors: T
authors: Mikami
authors: Jiro
authors: J
authors: Maegawa
mesh_terms: D000368:Aged; D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000166:cytology; D002469:Cell Separation; D002478:Cells, Cultured; D000078404:Epidermal Cells; D006801:Humans; D008969:Molecular Sequence Data; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000187:drug effects* / Q000637:transplantation; D064231:Nestin / Q000032:analysis*; D055495:Neurogenesis / Q000187:drug effects*; D009474:Neurons / Q000166:cytology; D010455:Peptides / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D017208:Rats, Wistar; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D064231:Nestin; D010455:Peptides; D054322:tat Gene Products, Human Immunodeficiency Virus
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