title: Tissue-specific, development-dependent phenolic compounds accumulation profile and gene expression pattern in tea plant [Camellia sinensis].
abstract: Phenolic compounds in tea plant [Camellia sinensis (L.)] play a crucial role in dominating tea flavor and possess a number of key pharmacological benefits on human health. The present research aimed to study the profile of tissue-specific, development-dependent accumulation pattern of phenolic compounds in tea plant. A total of 50 phenolic compounds were identified qualitatively using liquid chromatography in tandem mass spectrometry technology. Of which 29 phenolic compounds were quantified based on their fragmentation behaviors. Most of the phenolic compounds were higher in the younger leaves than that in the stem and root, whereas the total amount of proanthocyanidins were unexpectedly higher in the root. The expression patterns of 63 structural and regulator genes involved in the shikimic acid, phenylpropanoid, and flavonoid pathways were analyzed by quantitative real-time polymerase chain reaction and cluster analysis. Based on the similarity of their expression patterns, the genes were classified into two main groups: C1 and C2; and the genes in group C1 had high relative expression level in the root or low in the bud and leaves. The expression patterns of genes in C2-2-1 and C2-2-2-1 groups were probably responsible for the development-dependent accumulation of phenolic compounds in the leaves. Enzymatic analysis suggested that the accumulation of catechins was influenced simultaneously by catabolism and anabolism. Further research is recommended to know the expression patterns of various genes and the reason for the variation in contents of different compounds in different growth stages and also in different organs.
authors: Key Laboratory of Tea Biochemistry and Biotechnology, Ministry of Education in China, Anhui Agricultural University, Hefei, Anhui, China.
authors: Xiaolan
authors: X
authors: Jiang
authors: Yajun
authors: Y
authors: Liu
authors: Weiwei
authors: W
authors: Li
authors: Lei
authors: L
authors: Zhao
authors: Fei
authors: F
authors: Meng
authors: Yunsheng
authors: Y
authors: Wang
authors: Huarong
authors: H
authors: Tan
authors: Hua
authors: H
authors: Yang
authors: Chaoling
authors: C
authors: Wei
authors: Xiaochun
authors: X
authors: Wan
authors: Liping
authors: L
authors: Gao
authors: Tao
authors: T
authors: Xia
mesh_terms: D053898:Biosynthetic Pathways; D028241:Camellia sinensis / Q000737:chemistry* / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D016000:Cluster Analysis; D004798:Enzymes / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D013058:Mass Spectrometry; D005810:Multigene Family; D009928:Organ Specificity / Q000235:genetics; D010636:Phenols / Q000737:chemistry* / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D013662:Tea / Q000737:chemistry* / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D059467:Transcriptome
chemical_list: D004798:Enzymes; D010636:Phenols; D010936:Plant Extracts; D013662:Tea
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