title: Quercetin alleviates inflammation after short-term treatment in high-fat-fed mice.
abstract: Consumption of a high-fat diet (HFD) promotes reactive oxygen species (ROS) which ultimately trigger inflammation. The aim of this study was to investigate the role of Moringa oleifera leaf extract (MoLE) and its active component quercetin in preventing NF-κB-mediated inflammation raised by short-term HFD. Quercetin was found to be one of the major flavonoid components from HPLC of MoLE. Swiss mice were fed for 15 days on HFD, both with or without MoLE/quercetin. The antioxidant profile was estimated from liver homogenate. NF-κB and some relevant inflammatory markers were evaluated by immunoblotting, RT-PCR and ELISA. Significantly (P < 0.05) lower antioxidant profile and higher lipid peroxidation was found in HFD group compared to control (P < 0.05). Increased nuclear import of NF-κB and elevated expressions of pro-inflammatory markers were further manifestations in the HFD group. All these changes were reversed in the MoLE/quercetin-treated groups with significant improvement of antioxidant activity compared to the HFD group. MoLE was found to be rich in polyphenols and both MoLE and quercetin showed potent free radical and hydroxyl radical quenching activity. Thus, the present study concluded that short-term treatment with MoLE and its constituent quercetin prevent HFD-mediated inflammation in mice.
authors: Department of Physiology, UCST, University of Calcutta, 92 APC Road, Kolkata 700 009, India.
authors: Nilanjan
authors: N
authors: Das
authors: Kunal
authors: K
authors: Sikder
authors: Surajit
authors: S
authors: Bhattacharjee
authors: Suchandra Bhattacharya
authors: SB
authors: Majumdar
authors: Santinath
authors: S
authors: Ghosh
authors: Subrata
authors: S
authors: Majumdar
authors: Sanjit
authors: S
authors: Dey
mesh_terms: D000818:Animals; D059305:Diet, High-Fat / Q000009:adverse effects*; D006801:Humans; D007249:Inflammation / Q000188:drug therapy* / Q000209:etiology / Q000235:genetics / Q000276:immunology; D008099:Liver / Q000276:immunology; D008297:Male; D051379:Mice; D029588:Moringa oleifera / Q000737:chemistry*; D016328:NF-kappa B / Q000235:genetics / Q000276:immunology; D010936:Plant Extracts / Q000008:administration & dosage*; D011794:Quercetin / Q000008:administration & dosage*
chemical_list: D016328:NF-kappa B; D010936:Plant Extracts; D011794:Quercetin