title: Salt stress encourages proline accumulation by regulating proline biosynthesis and degradation in Jerusalem artichoke plantlets.
abstract: Proline accumulation is an important mechanism for osmotic regulation under salt stress. In this study, we evaluated proline accumulation profiles in roots, stems and leaves of Jerusalem artichoke (Helianthus tuberosus L.) plantlets under NaCl stress. We also examined HtP5CS, HtOAT and HtPDH enzyme activities and gene expression patterns of putative HtP5CS1, HtP5CS2, HtOAT, HtPDH1, and HtPDH2 genes. The objective of our study was to characterize the proline regulation mechanisms of Jerusalem artichoke, a moderately salt tolerant species, under NaCl stress. Jerusalem artichoke plantlets were observed to accumulate proline in roots, stems and leaves during salt stress. HtP5CS enzyme activities were increased under NaCl stress, while HtOAT and HtPDH activities generally repressed. Transcript levels of HtP5CS2 increased while transcript levels of HtOAT, HtPDH1 and HtPDH2 generally decreased in response to NaCl stress. Our results supports that for Jerusalem artichoke, proline synthesis under salt stress is mainly through the Glu pathway, and HtP5CS2 is predominant in this process while HtOAT plays a less important role. Both HtPDH genes may function in proline degradation.
authors: Jiangsu Key Lab of Marine Biology, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China.
authors: Zengrong
authors: Z
authors: Huang
authors: Long
authors: L
authors: Zhao
authors: Dandan
authors: D
authors: Chen
authors: Mingxiang
authors: M
authors: Liang
authors: Zhaopu
authors: Z
authors: Liu
authors: Hongbo
authors: H
authors: Shao
authors: Xiaohua
authors: X
authors: Long
mesh_terms: D019295:Computational Biology; D018556:Crops, Agricultural / Q000201:enzymology / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D020869:Gene Expression Profiling; D017343:Genes, Plant / Q000235:genetics; D006368:Helianthus / Q000201:enzymology / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D011392:Proline / Q000096:biosynthesis* / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology*; D013312:Stress, Physiological / Q000187:drug effects* / Q000235:genetics
chemical_list: D012965:Sodium Chloride; D011392:Proline
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