title: Cyclin d1 expression in odontogenic cysts.
abstract: OBJECTIVE
  In the present study expression of cyclin D1 in the epithelial lining of odontogenic keratocyst, radicular cyst, dentigerous cyst and glandular odontogenic cyst was investigated to compare proliferative activity in these lesions.
  
  
  METHODS
  Immunohistochemical staining of cyclin D1 on formalin-fixed, paraffin-embedded tissue sections of odontogenic keratocysts (n=23), dentigerous cysts (n=20), radicular cysts (n=20) and glandular odontogenic cysts (n=5) was performed by standard EnVision method. Then, slides were studied to evaluate the following parameters in epithelial lining of cysts: expression, expression pattern, staining intensity and localization of expression.
  
  
  RESULTS
  The data analysis showed statistically significant difference in cyclin D1 expression in studied groups (p < 0.001). Assessment of staining intensity and staining pattern showed more strong intensity and focally pattern in odontogenic keratocysts, but difference was not statistically significant among groups respectively (p=0.204, 0.469). Considering expression localization, cyclin D1 positive cells in odontogenic keratocysts and dentigerous cysts were frequently confined in parabasal layer, different from radicular cysts and glandular odontogenic cysts. The difference was statistically significant (p < 0.01).
  
  
  CONCLUSIONS
  Findings showed higher expression of cyclin D1 in parabasal layer of odontogenic keratocyst and the entire cystic epithelium of glandular odontogenic cysts comparing to dentigerous cysts and radicular cysts, implying the possible role of G1-S cell cycle phase disturbances in the aggressiveness of odontogenic keratocyst and glandular odontogenic cyst.
authors: Department of Oral and Maxillofacial Pathology, Shahid Beheshti University of Medical Science, Tehran, Iran. nasim.taghavi@yahoo.com
authors: Nasim
authors: N
authors: Taghavi
authors: Shirin
authors: S
authors: Modabbernia
authors: Alireza
authors: A
authors: Akbarzadeh
authors: Samad
authors: S
authors: Sajjadi
mesh_terms: D000328:Adult; D001706:Biopsy; D002453:Cell Cycle; D049109:Cell Proliferation; D019938:Cyclin D1 / Q000032:analysis*; D003803:Dentigerous Cyst / Q000737:chemistry / Q000473:pathology; D004847:Epithelial Cells / Q000737:chemistry* / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D009807:Odontogenic Cysts / Q000737:chemistry* / Q000473:pathology; D011842:Radicular Cyst / Q000737:chemistry / Q000473:pathology
chemical_list: C530104:CCND1 protein, human; D019938:Cyclin D1