title: p-Hydroxycinnamic acid production directly from cellulose using endoglucanase- and tyrosine ammonia lyase-expressing Streptomyces lividans.
abstract: BACKGROUND
  p-Hydroxycinnamic acid (pHCA) is an aromatic compound that serves as a starting material for the production of many commercially valuable chemicals, such as fragrances and pharmaceuticals, and is also used in the synthesis of thermostable polymers. However, chemical synthesis of pHCA is both costly and harmful to the environment. Although pHCA production using microbes has been widely studied, there remains a need for more cost-effective methods, such as the use of biomass as a carbon source. In this study, we produced pHCA using tyrosine ammonia lyase-expressing Streptomyces lividans. In order to improve pHCA productivity from cellulose, we constructed a tyrosine ammonia lyase- and endoglucanase (EG)-expressing S. lividans transformant and used it to produce pHCA from cellulose.
  
  
  RESULTS
  A Streptomyces lividans transformant was constructed to express tyrosine ammonia lyase derived from Rhodobacter sphaeroides (RsTAL). The transformant produced 786 or 736 mg/L of pHCA after 7 days of cultivation in medium containing 1% glucose or cellobiose as the carbon source, respectively. To enhance pHCA production from phosphoric acid swollen cellulose (PASC), we introduced the gene encoding EG into RsTAL-expressing S. lividans. After 7 days of cultivation, this transformant produced 753, 743, or 500 mg/L of pHCA from 1% glucose, cellobiose, or PASC, respectively.
  
  
  CONCLUSIONS
  RsTAL-expressing S. lividans can produce pHCA from glucose and cellobiose. Similarly, RsTAL- and EG-expressing S. lividans can produce pHCA from glucose and cellobiose with excess EG activity remaining in the supernatant. This transformant demonstrated improved pHCA production from cellulose. Further enhancements in the cellulose degradation capability of the transformant will be necessary in order to achieve further improvements in pHCA production from cellulose.
authors: Yoshifumi
authors: Y
authors: Kawai
authors: Shuhei
authors: S
authors: Noda
authors: Chiaki
authors: C
authors: Ogino
authors: Yasunobu
authors: Y
authors: Takeshima
authors: Naoko
authors: N
authors: Okai
authors: Tsutomu
authors: T
authors: Tanaka
authors: Akihiko
authors: A
authors: Kondo
mesh_terms: D000642:Ammonia-Lyases / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002475:Cellobiose / Q000378:metabolism; D002480:Cellulase / Q000235:genetics* / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D003373:Coumaric Acids / Q000737:chemistry / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011422:Propionates; D012242:Rhodobacter sphaeroides / Q000201:enzymology / Q000235:genetics; D048170:Streptomyces lividans / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D003373:Coumaric Acids; D011422:Propionates; D002475:Cellobiose; D002482:Cellulose; D002480:Cellulase; D000642:Ammonia-Lyases; C023208:L-tyrosine ammonia-lyase; C495469:p-coumaric acid; D005947:Glucose
references: Appl Microbiol Biotechnol. 2007 Mar;74(3):617-24
references: 17111138
references: Nat Biotechnol. 1996 Jun;14(6):756-9
references: 9630985
references: Bioresour Technol. 2009 Nov;100(21):5155-61
references: 19540109
references: Trends Biotechnol. 1997 Aug;15(8):315-20
references: 9263479
references: Bioresour Technol. 2012 Jan;104:648-51
references: 22115528
references: Biotechnol Biofuels. 2010 Jul 02;3:16
references: 20598120
references: J Ind Microbiol Biotechnol. 2011 May;38(5):643-8
references: 21424686
references: Enzyme Microb Technol. 2012 Dec 10;51(6-7):366-72
references: 23040393
references: J Bacteriol. 2008 Apr;190(8):2822-30
references: 17993537
references: J Ind Microbiol Biotechnol. 2007 Sep;34(9):599-604
references: 17602252
references: FEMS Microbiol Lett. 2010 Oct;311(1):93-101
references: 20727010
references: Proc Natl Acad Sci U S A. 2012 Aug 14;109(33):13260-5
references: 22853950
references: Proc Natl Acad Sci U S A. 2011 Dec 13;108(50):19949-54
references: 22123987
references: Microb Cell Fact. 2011 Dec 20;10:110
references: 22185589
references: Microb Cell Fact. 2012 Apr 30;11:49
references: 22545774
references: Biotechnol Bioeng. 2001 Dec;76(4):376-90
references: 11745165
references: Chem Biol. 2006 Dec;13(12):1327-38
references: 17185228
references: Bioresour Technol. 2010 Jul;101(13):4767-74
references: 19944601
references: Biomaterials. 2005 Nov;26(32):6263-70
references: 15913768
references: J Antibiot (Tokyo). 2003 Nov;56(11):950-6
references: 14763561
references: Curr Opin Biotechnol. 2009 Dec;20(6):651-8
references: 19875279
references: Protein Expr Purif. 2010 Oct;73(2):198-202
references: 20546899
references: Appl Environ Microbiol. 2009 Feb;75(4):931-6
references: 19060171
references: Metab Eng. 2007 Mar;9(2):142-51
references: 17204442