title: Identification of rice β-glucosidase with high hydrolytic activity towards salicylic acid β-D-glucoside.
abstract: β-Glucosidases (EC 3.2.1.21) split β-glucosidic linkages at the non-reducing end of glucosides and oligosaccharides to release β-D-glucose. One of the important functions of plant β-glucosidase is deglucosylation of inactive glucosides of phytohormones to regulate levels of active hormones. Tuberonic acid is a jasmonate-related compound that shows tuber-inducing activity in the potato. We have identified two enzymes, OsTAGG1 and OsTAGG2, that have hydrolytic activity towards tuberonic acid β-D-glucoside in rice (Oryza sativa L.). The expression of OsTAGG2 is upregulated by wounding and by methyl jasmonate, suggesting that this isozyme is involved in responses to biotic stresses and wounding, but the physiological substrate of OsTAGG2 remains ambiguous. In this study, we produced recombinant OsTAGG2 in Pichia pastoris (rOsTAGG2P), and investigated its substrate specificity in detail. From 1 L of culture medium, 2.1 mg of purified recombinant enzyme was obtained by ammonium sulfate precipitation and Ni-chelating column chromatography. The specific activity of rOsTAGG2P (182 U/mg) was close to that of the native enzyme (171 U/mg), unlike recombinant OsTAGG2 produced in Escherichia coli, which had approximately 3-fold lower specific activity than the native enzyme. The optimum pH and temperature for rOsTAGG2P were pH 3.4 and 60 °C. After pH and heat treatments, the enzyme retained its original activity in a pH range of 3.4-9.8 and below 55 °C. Native OsTAGG2 and rOsTAGG2P showed 4.5-4.7-fold higher activities towards salicylic acid β-D-glucoside, an inactive storage-form of salicylic acid, than towards tuberonic acid β-D-glucoside (TAG), although OsTAGG2 was originally isolated from rice based on TAG-hydrolytic activity.
authors: Research Faculty of Agriculture, Hokkaido University, Kita-ku, Sapporo, Japan.
authors: Nami
authors: N
authors: Himeno
authors: Wataru
authors: W
authors: Saburi
authors: Shinji
authors: S
authors: Wakuta
authors: Ryosuke
authors: R
authors: Takeda
authors: Hideyuki
authors: H
authors: Matsuura
authors: Kensuke
authors: K
authors: Nabeta
authors: Sompong
authors: S
authors: Sansenya
authors: James R
authors: JR
authors: Ketudat Cairns
authors: Haruhide
authors: H
authors: Mori
authors: Ryozo
authors: R
authors: Imai
authors: Hirokazu
authors: H
authors: Matsui
mesh_terms: D000595:Amino Acid Sequence; D005960:Glucosides / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology*; D010843:Pichia / Q000235:genetics; D012459:Salicylates / Q000378:metabolism*; D013379:Substrate Specificity; D013696:Temperature; D001617:beta-Glucosidase / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D005960:Glucosides; D012459:Salicylates; C084746:salicylic acid glucoside; D001617:beta-Glucosidase