title: Convection-enhanced delivery of targeted quantum dot-immunoliposome hybrid nanoparticles to intracranial brain tumor models.
abstract: OBJECTIVE
  The aim of this work is to evaluate combining targeting strategy and convection-enhanced delivery in brain tumor models by imaging quantum dot-immunoliposome hybrid nanoparticles.
  
  
  METHODS
  An EGF receptor-targeted, quantum dot-immunoliposome hybrid nanoparticle (QD-IL) was synthesized. In vitro uptake was measured by flow cytometry and intracellular localization was imaged by confocal microscopy. In the in vivo study, QD-ILs were delivered to intracranial xenografts via convection-enhanced delivery and fluorescence was monitored noninvasively in real-time.
  
  
  RESULTS
  QD-ILs exhibited specific and efficient uptake in vitro and exhibited approximately 1.3- to 5.0-fold higher total fluorescence compared with nontargeted counterpart in intracranial brain tumor xenografts in vivo.
  
  
  CONCLUSIONS
  QD-ILs serve as an effective imaging agent in vitro and in vivo, and the data suggest that ligand-directed liposomal nanoparticles in conjunction with convection-enhanced delivery may offer therapeutic benefits for glioblastoma treatment as a result of specific and efficient uptake by malignant cells.
authors: UCSF Helen Diller Family Comprehensive Cancer Center, San Francisco, CA 94115, USA.
authors: Kevin C
authors: KC
authors: Weng
authors: Rintaro
authors: R
authors: Hashizume
authors: Charles O
authors: CO
authors: Noble
authors: Laura P
authors: LP
authors: Serwer
authors: Daryl C
authors: DC
authors: Drummond
authors: Dmitri B
authors: DB
authors: Kirpotin
authors: Anne M
authors: AM
authors: Kuwabara
authors: Lucy X
authors: LX
authors: Chao
authors: Fanqing F
authors: FF
authors: Chen
authors: Charles D
authors: CD
authors: James
authors: John W
authors: JW
authors: Park
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism / Q000473:pathology*; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D018481:Convection; D016503:Drug Delivery Systems; D066246:ErbB Receptors / Q000378:metabolism*; D005260:Female; D005909:Glioblastoma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008081:Liposomes / Q000032:analysis / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D045663:Quantum Dots / Q000032:analysis / Q000378:metabolism*
chemical_list: D008081:Liposomes; D066246:ErbB Receptors