title: Experimental etch-and-rinse adhesives doped with bioactive calcium silicate-based micro-fillers to generate therapeutic resin-dentin interfaces.
abstract: OBJECTIVE
  This study aimed at evaluating the therapeutic bioactive effects on the bond strength of three experimental bonding agents containing modified Portland cement-based micro-fillers applied to acid-etched dentin and submitted to aging in simulated body fluid solution (SBS). Confocal laser (CLSM) and scanning electron microscopy (SEM) were also performed.
  
  
  METHODS
  A type-I ordinary Portland cement was tailored using different compounds such as sodium-calcium-aluminum-magnesium silicate hydroxide (HOPC), aluminum-magnesium-carbonate hydroxide hydrates (HCPMM) and titanium oxide (HPCTO) to create three bioactive micro-fillers. A resin blend mainly constituted by Bis-GMA, PMDM and HEMA was used as control (RES-Ctr) or mixed with each micro-filler to create three experimental bonding agents: (i) Res-HOPC, (ii) Res-HCPMM and (iii) Res-HPCTO. The bonding agents were applied onto 37% H3PO4-etched dentin and light-cured for 30s. After build-ups, they were prepared for micro-tensile bond strength (μTBS) and tested after 24h or 6 months of SBS storage. SEM analysis was performed after de-bonding, while CLSM was used to evaluate the ultra-morphology/nanoleakage and the mineral deposition at the resin-dentin interface.
  
  
  RESULTS
  High μTBS values were achieved in all groups after 24h. Only Res-HOPC and Res-HCPMM showed stable μTBS after SBS storage (6 months). All the resin-dentin interfaces created using the bonding agents containing the bioactive micro-fillers tested in this study showed an evident reduction of nanoleakage and mineral deposition after SBS storage.
  
  
  CONCLUSIONS
  Resin bonding systems containing specifically tailored Portland cement micro-fillers may promote a therapeutic mineral deposition within the hybrid layer and increase the durability of the resin-dentin bond.
authors: Biomaterials, Biomimetics and Biophotonics, King's College London Dental Institute, Guy's, King's College and St. Thomas' Hospital, London SE1 9RT, UK.
authors: A C
authors: AC
authors: Profeta
authors: F
authors: F
authors: Mannocci
authors: R
authors: R
authors: Foxton
authors: T F
authors: TF
authors: Watson
authors: V P
authors: VP
authors: Feitosa
authors: B
authors: B
authors: De Carlo
authors: R
authors: R
authors: Mongiorgi
authors: G
authors: G
authors: Valdré
authors: S
authors: S
authors: Sauro
mesh_terms: D000269:Adhesives / Q000737:chemistry; D000328:Adult; D001826:Body Fluids; D017610:Calcium Compounds; D001840:Dental Bonding; D003738:Dental Cements / Q000737:chemistry*; D003763:Dental Leakage / Q000517:prevention & control; D003799:Dental Stress Analysis; D003804:Dentin / Q000737:chemistry; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D004356:Drug Storage; D006801:Humans; D008422:Materials Testing; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D010316:Particle Size; D019279:Resin Cements / Q000737:chemistry*; D017640:Silicates; D013718:Tensile Strength; D014089:Tooth Remineralization; D055815:Young Adult
chemical_list: D000269:Adhesives; D017610:Calcium Compounds; D003738:Dental Cements; D017220:Dentin-Bonding Agents; D019279:Resin Cements; D017640:Silicates; C472247:accelerated Portland cement; C031293:calcium silicate