title: Characterization of sex determination and sex differentiation genes in Latimeria.
abstract: Genes involved in sex determination and differentiation have been identified in mice, humans, chickens, reptiles, amphibians and teleost fishes. However, little is known of their functional conservation, and it is unclear whether there is a common set of genes shared by all vertebrates. Coelacanths, basal Sarcopterygians and unique "living fossils", could help establish an inventory of the ancestral genes involved in these important developmental processes and provide insights into their components. In this study 33 genes from the genome of Latimeria chalumnae and from the liver and testis transcriptomes of Latimeria menadoensis, implicated in sex determination and differentiation, were identified and characterized and their expression levels measured. Interesting findings were obtained for GSDF, previously identified only in teleosts and now characterized for the first time in the sarcopterygian lineage; FGF9, which is not found in teleosts; and DMRT1, whose expression in adult gonads has recently been related to maintenance of sexual identity. The gene repertoire and testis-specific gene expression documented in coelacanths demonstrate a greater similarity to modern fishes and point to unexpected changes in the gene regulatory network governing sexual development.
authors: Dipartimento di Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, Ancona, Italy.
authors: Mariko
authors: M
authors: Forconi
authors: Adriana
authors: A
authors: Canapa
authors: Marco
authors: M
authors: Barucca
authors: Maria A
authors: MA
authors: Biscotti
authors: Teresa
authors: T
authors: Capriglione
authors: Francesco
authors: F
authors: Buonocore
authors: Anna M
authors: AM
authors: Fausto
authors: Daisy M
authors: DM
authors: Makapedua
authors: Alberto
authors: A
authors: Pallavicini
authors: Marco
authors: M
authors: Gerdol
authors: Gianluca
authors: G
authors: De Moro
authors: Giuseppe
authors: G
authors: Scapigliati
authors: Ettore
authors: E
authors: Olmo
authors: Manfred
authors: M
authors: Schartl
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005399:Fishes / Q000235:genetics* / Q000254:growth & development*; D020869:Gene Expression Profiling; D023281:Genomics; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D019849:Sex Determination Processes / Q000235:genetics*; D012733:Sex Differentiation / Q000235:genetics*; D016212:Transforming Growth Factor beta / Q000737:chemistry
chemical_list: D029941:Fish Proteins; D016212:Transforming Growth Factor beta
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