title: Utilization of lactic acid bacterial genes in Synechocystis sp. PCC 6803 in the production of lactic acid.
abstract: Metabolic pathway engineering of cyanobacteria for the production of industrially important chemicals from atmospheric CO2 has generated interest recently. Here, we engineered Synechocystis sp. PCC 6803 to produce lactic acid using a lactate dehydrogenase (ldh) gene from various lactic acid-producing bacteria, Lactococcus lactis (ldhB and ldhX), Lactobacillus plantarum (ldhL and ldh), and Lactobacillus rhamnosus (ldhL). The lactic acid was secreted outside the cell using a transporter (lldp) gene from L. plantarum. Expression of each ldh in Synechocystis sp. PCC6803 was ascertained by reverse-transcriptase polymerase chain reaction. Five transformants led to the production of L-lactic acid. Co-expression of lldp with ldhB from L. plantarum or ldhL from L. rhamnosus led to the secretion of lactic acid into the medium at concentration of 0.17 ± 0.02 or 0.14 ± 0.02 mM after 18 d of cultivation.
authors: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Rokkodaicho, Nada-ku, Kobe, Hyogo, Japan.
authors: Ancy
authors: A
authors: Joseph
authors: Shimpei
authors: S
authors: Aikawa
authors: Kengo
authors: K
authors: Sasaki
authors: Yota
authors: Y
authors: Tsuge
authors: Fumio
authors: F
authors: Matsuda
authors: Tsutomu
authors: T
authors: Tanaka
authors: Akihiko
authors: A
authors: Kondo
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D005818:Genetic Engineering / Q000379:methods*; D007770:L-Lactate Dehydrogenase / Q000235:genetics*; D019344:Lactic Acid / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D007778:Lactobacillus / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D017045:Lactococcus / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D027501:Monocarboxylic Acid Transporters / Q000235:genetics; D013237:Stereoisomerism; D046939:Synechocystis / Q000166:cytology / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*; D014169:Transformation, Bacterial
chemical_list: D027501:Monocarboxylic Acid Transporters; D019344:Lactic Acid; D007770:L-Lactate Dehydrogenase