title: Vanillin inhibits translation and induces messenger ribonucleoprotein (mRNP) granule formation in saccharomyces cerevisiae: application and validation of high-content, image-based profiling.
abstract: Vanillin, generated by acid hydrolysis of lignocellulose, acts as a potent inhibitor of the growth of the yeast Saccharomyces cerevisiae. Here, we investigated the cellular processes affected by vanillin using high-content, image-based profiling. Among 4,718 non-essential yeast deletion mutants, the morphology of those defective in the large ribosomal subunit showed significant similarity to that of vanillin-treated cells. The defects in these mutants were clustered in three domains of the ribosome: the mRNA tunnel entrance, exit and backbone required for small subunit attachment. To confirm that vanillin inhibited ribosomal function, we assessed polysome and messenger ribonucleoprotein granule formation after treatment with vanillin. Analysis of polysome profiles showed disassembly of the polysomes in the presence of vanillin. Processing bodies and stress granules, which are composed of non-translating mRNAs and various proteins, were formed after treatment with vanillin. These results suggest that vanillin represses translation in yeast cells.
authors: The Department of Applied Biology, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Kyoto, Japan.
authors: Aya
authors: A
authors: Iwaki
authors: Shinsuke
authors: S
authors: Ohnuki
authors: Yohei
authors: Y
authors: Suga
authors: Shingo
authors: S
authors: Izawa
authors: Yoshikazu
authors: Y
authors: Ohya
mesh_terms: D001547:Benzaldehydes / Q000494:pharmacology*; D003594:Cytoplasmic Granules / Q000378:metabolism*; D004875:Ergosterol / Q000096:biosynthesis; D008958:Models, Molecular; D008968:Molecular Conformation; D009154:Mutation; D011132:Polyribosomes / Q000378:metabolism; D021381:Protein Transport / Q000187:drug effects; D012261:Ribonucleoproteins / Q000378:metabolism*; D054658:Ribosome Subunits, Large / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*
chemical_list: D001547:Benzaldehydes; D012261:Ribonucleoproteins; C026452:messenger ribonucleoprotein; C100058:vanillin; D004875:Ergosterol
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