title: Feedstocks affect the diversity and distribution of propionate CoA-transferase genes (pct) in anaerobic digesters.
abstract: Anaerobic digestion (AD) is an attractive microbiological technology for both waste treatment and energy production. Syntrophic acetogenic bacteria are an important guild because they are essential for maintaining efficient and stable AD operation. However, this guild is poorly understood due to difficulties to culture them. In this study, we developed specific PCR assays targeting the propionate-CoA transferase genes (pct) to investigate their diversity and distribution in several mesophilic anaerobic digesters and a bench-scale temperature-phased AD (TPAD) system. Phylogenetic analysis of sequenced pct amplicons revealed the occurrence of Syntrophobacter fumaroxidans and six other clusters of putative pct genes. Principal coordinate analysis (PCoA) showed that pct diversity and abundance were largely correlated to the feedstocks of the digesters, while little difference was seen between the granular and the liquid fractions of each digester or between the two digesters of the TPAD system. Cluster-specific qPCR analysis revealed major impact of feedstocks and fractions on the abundance of pct genes. Readily fermentable substrates such as sugar- or starch-rich feedstocks selected for pct genes (Cluster I) related to Syntrophobacter, while manure feedstock selected for pct clusters related to pct of Clostridium spp. These results suggest that propionate metabolism can be affected by feedstocks and partition differently between solid and liquid phases in digesters. The PCR assays developed in this study may serve as a tool to investigate propionate-oxidizing bacteria in anaerobic digesters and other anaerobic environments.
authors: Environmental Science Graduate Program, The Ohio State University, Columbus, OH 43210, USA.
authors: Yueh-Fen
authors: YF
authors: Li
authors: Shan
authors: S
authors: Wei
authors: Zhongtang
authors: Z
authors: Yu
mesh_terms: D000595:Amino Acid Sequence; D000693:Anaerobiosis; D001419:Bacteria / Q000145:classification / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D044822:Biodiversity; D019149:Bioreactors / Q000382:microbiology*; D015256:Coenzyme A-Transferases / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D012722:Sewage / Q000737:chemistry* / Q000382:microbiology
chemical_list: D001426:Bacterial Proteins; D012722:Sewage; D015256:Coenzyme A-Transferases; C031212:acyl-CoA transferase
references: Proc Natl Acad Sci U S A. 2007 May 1;104(18):7600-5
references: 17442750
references: Biotechniques. 2004 May;36(5):808-12
references: 15152600
references: Nucleic Acids Res. 2004 Feb 25;32(4):1363-71
references: 14985472
references: Environ Microbiol. 2001 Mar;3(3):194-204
references: 11321536
references: Syst Biol. 2008 Oct;57(5):785-94
references: 18853364
references: Appl Environ Microbiol. 2001 Mar;67(3):1392-5
references: 11229939
references: Mol Biol Evol. 1987 Jul;4(4):406-25
references: 3447015
references: Appl Environ Microbiol. 2010 Apr;76(7):2058-66
references: 20118378
references: J Biol Chem. 2005 Dec 30;280(52):42919-28
references: 16253988
references: Biotechnol Bioeng. 2004 Sep 30;87(7):823-34
references: 15334409
references: Int J Parasitol. 2010 Mar 15;40(4):387-97
references: 20085767
references: Environ Microbiol. 2005 Aug;7(8):1104-15
references: 16011748
references: Appl Environ Microbiol. 2005 Dec;71(12):8228-35
references: 16332807
references: Water Environ Res. 2006 May;78(5):460-73
references: 16752608
references: Nucleic Acids Res. 1994 Nov 11;22(22):4673-80
references: 7984417
references: Comput Appl Biosci. 1992 Jun;8(3):275-82
references: 1633570
references: Bioresour Technol. 2012 Mar;107:135-43
references: 22257856
references: Microbiology (Reading). 2002 Nov;148(Pt 11):3521-3530
references: 12427943
references: Syst Appl Microbiol. 2011 Jun;34(4):260-6
references: 21498020
references: Appl Environ Microbiol. 1996 May;62(5):1656-63
references: 8633864
references: Water Res. 2007 Apr;41(7):1554-68
references: 17291558
references: Environ Microbiol. 2010 Aug;12(8):2289-301
references: 21966920
references: Appl Environ Microbiol. 2006 Apr;72(4):2400-6
references: 16597937
references: Syst Appl Microbiol. 2005 Mar;28(2):175-85
references: 15830810
references: Microb Ecol. 2012 Jan;63(1):32-40
references: 21811793
references: J Bacteriol. 2006 Jan;188(1):202-10
references: 16352836
references: Appl Microbiol Biotechnol. 2013 Sep;97(17):7699-709
references: 23250223
references: Appl Environ Microbiol. 2008 Jun;74(11):3610-4
references: 18408059
references: Methods Enzymol. 2005;397:454-69
references: 16260309
references: Appl Environ Microbiol. 2001 Apr;67(4):1800-4
references: 11282636
references: Biotechnol Bioeng. 2011 Feb;108(2):296-305
references: 20830676
references: Bioresour Technol. 2010 Dec;101(24):9461-70
references: 20705457
references: Nucleic Acids Res. 2003 Jul 1;31(13):3763-6
references: 12824413
references: Appl Environ Microbiol. 1999 Mar;65(3):1280-8
references: 10049894
references: Appl Environ Microbiol. 2010 Dec;76(23):7785-95
references: 20889794
references: Appl Environ Microbiol. 1991 Jun;57(6):1764-9
references: 1872605
references: Int J Syst Bacteriol. 1998 Oct;48 Pt 4:1383-7
references: 9828440