title: Identification of alkylbenzene sulfonate surfactants leaching from an acrylonitrile butadiene rubber as novel inhibitors of calcineurin activity.
abstract: Calcineurin (CN) is a Ca(2+)/calmodulin (CaM) dependent serine/threonine protein phosphatase and plays important role in several cellular functions in both higher and lower eukaryotes. Here we report inhibition of CN by linear alkylbenzene sulfonate. The clue to the finding was obtained while identifying the inhibitory material leaching from acrylonitrile butadiene rubber used for packing. Using standard dodecylbenzene sulfonate (C12-LAS), we obtained strong inhibition of CN with a half maximal inhibitory concentration of 9.3 µM, whereas analogs such as p-octylbenzene sulfonate and SDS hardly or only slightly affected CN activity. Three alkaline phosphatases, derived from shrimp, bacteria, and calf-intestine, which exhibit similar enzymatic activities to CN, were not inhibited by C12-LAS at concentrations of up to 100 µM. Furthermore, C12-LAS did not inhibit Ca(2+)/CaM-dependent myosin light chain kinase activity when tested at concentrations of up to 36 µM. The results indicate that C12-LAS is a potent selective inhibitor of CN activity.
authors: Department of Laboratory Sciences, Gunma University Graduate School of Health Sciences, Maebashi, Japan.
authors: Noboru
authors: N
authors: Ito
authors: Nao
authors: N
authors: Shibuguchi
authors: Ryoki
authors: R
authors: Ishikawa
authors: Susumu
authors: S
authors: Tanaka
authors: Yoshiharu
authors: Y
authors: Tokita
authors: Junko
authors: J
authors: Nakajima-Shimada
authors: Kohei
authors: K
authors: Hosaka
mesh_terms: D000181:Acrylonitrile / Q000737:chemistry*; D000818:Animals; D001554:Benzene / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002070:Butadienes / Q000737:chemistry*; D065095:Calcineurin Inhibitors; D002417:Cattle; D004353:Drug Evaluation, Preclinical; D004791:Enzyme Inhibitors / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000431:Ethanol / Q000737:chemistry; D012408:Rubber / Q000737:chemistry*; D013501:Surface-Active Agents / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D002070:Butadienes; D065095:Calcineurin Inhibitors; D004791:Enzyme Inhibitors; D013501:Surface-Active Agents; D000431:Ethanol; D012408:Rubber; D001554:Benzene; D000181:Acrylonitrile