title: Transformation of the fungus Absidia glauca by complementation of a methionine-auxotrophic strain affected in the homoserine-acetyltransferase gene.
abstract: Transformation of fungi by complementation of auxotrophs is generally much more reliable than usage of antibiotic resistance markers. In order to establish such a system for the model zygomycete Absidia glauca, a stable methionine auxotrophic mutant was isolated after X-ray mutagenesis of the minus mating type and characterized at the molecular level. The mutant is disrupted in the coding region of the Met2-1 gene, encoding homoserine O-acetyltransferase. The corresponding wild type gene was cloned, sequenced and inserted into appropriate vector plasmids. Transformants are prototrophs and show restored methionine-independent growth, based on complementation by the autonomously replicating plasmids.
authors: General Microbiology and Microbe Genetics, Friedrich-Schiller University Jena, Neugasse 24, D-07743 Jena, Germany.
authors: Sedighe
authors: S
authors: Karimi
authors: Jana
authors: J
authors: Wetzel
authors: Johannes
authors: J
authors: Wöstemeyer
authors: Anke
authors: A
authors: Burmester
keywords: Homoserine O-acetyltransferase; Met2; Methionine biosynthesis; Mucor-like fungi; Zygomycetes
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