title: Lack of effect of bioactive-rich extracts of pomegranate, persimmon, nettle, dill, kale and Sideritis and isolated bioactives on platelet function.
abstract: BACKGROUND
  The health benefits of fruit and vegetable-rich diets may be partly due to modulation of platelet activity by bioactive phytochemicals. The aim of this study was to investigate the effects of bioactive-rich plant extracts and isolated bioactive metabolites on platelet function. Blood samples (n =15 subjects) were treated with extracts of bioactive-rich plants consumed as traditional foods in the Black Sea region, or with human metabolites of the bioactives quercetin and sulforaphane. Platelet function was assessed using the PFA-100.
  
  
  RESULTS
  None of the extracts containing various flavonoids, glucosinolates and other bioactives, or isolated bioactive metabolites of quercetin or sulforaphane, caused significant changes in PFA-100 closure time (CT). In contrast, the positive controls (aspirin and Abciximab) consistently caused significant increases in CT for the platelet agonists epinephrine and ADP, respectively.
  
  
  CONCLUSIONS
  These data do not support the notion that these plant bioactives can improve human platelet function.
authors: Institute of Food Research, Norwich Research Park, Norwich, NR4 7UA, UK.
authors: Wendy J
authors: WJ
authors: Hollands
authors: Shikha
authors: S
authors: Saha
authors: Osman
authors: O
authors: Hayran
authors: Nadiya
authors: N
authors: Boyko
authors: Maria
authors: M
authors: Glibetic
authors: Aleksandra
authors: A
authors: Konic-Ristic
authors: Mariam
authors: M
authors: Jorjadze
authors: Paul A
authors: PA
authors: Kroon
mesh_terms: D000328:Adult; D028524:Anethum graveolens / Q000737:chemistry; D059038:Black Sea; D001792:Blood Platelets / Q000187:drug effects* / Q000502:physiology*; D001937:Brassica / Q000737:chemistry; D003469:Culture; D029788:Diospyros / Q000737:chemistry; D005260:Female; D005419:Flavonoids / Q000032:analysis; D005502:Food; D005638:Fruit / Q000737:chemistry*; D005961:Glucosinolates / Q000032:analysis; D006801:Humans; D017879:Isothiocyanates / Q000494:pharmacology; D029561:Lythraceae / Q000737:chemistry; D008297:Male; D008875:Middle Aged; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D010979:Platelet Function Tests; D059808:Polyphenols / Q000032:analysis; D011794:Quercetin / Q000494:pharmacology; D031365:Sideritis / Q000737:chemistry; D013454:Sulfoxides; D026982:Urtica dioica / Q000737:chemistry; D014675:Vegetables / Q000737:chemistry*
chemical_list: D005419:Flavonoids; D005961:Glucosinolates; D017879:Isothiocyanates; D010936:Plant Extracts; D010975:Platelet Aggregation Inhibitors; D059808:Polyphenols; D013454:Sulfoxides; D011794:Quercetin; C016766:sulforaphane
keywords: PFA-100; flavonoids; in vitro; platelets; polyphenols