title: Evaluation of genotoxic and cytotoxic effects of antileishmanial extract from Julocroton triqueter (Euphorbiaceae).
abstract: Julocroton triqueter extracts have antileishmanial activity; however, the effect on genetic stability has not been studied. We evaluated genotoxic and cell death induction potential (in vitro and in vivo) of J. triqueter var. triqueter hydroalcoholic extracts, as well as their antigenotoxic potential in vivo. The in vitro genotoxic studies were performed using human leukocytes at four different concentrations. For the in vivo tests, Swiss mice were treated with 125, 250 or 500 mg/kg of extract injected intraperitoneally. Antigenotoxic effects of the extract were measured before and after cyclophosphamide treatment. An absence of genotoxic effects was observed both in vitro and in vivo. In the antigenotoxic studies, no significant difference was observed between the treatments and the positive control, indicating that the extracts did not protect against damage caused by cyclophosphamide. Hydroalcoholic extracts of J. triqueter did not provoke DNA damage at concentrations and doses normally used for antileishmanial treatment; however, they reduced apoptotic cell death and induced necrotic cell death.
authors: Laboratório de Genética e Biologia Molecular, Departamento de Biologia, Universidade Federal do Maranhão, São Luís, MA, Brasil.
authors: V R
authors: VR
authors: Moreira
authors: A C S
authors: AC
authors: Dias
authors: A R P
authors: AR
authors: Martins
authors: M I S
authors: MI
authors: Lima
authors: I C P B
authors: IC
authors: Serra
authors: M N S
authors: MN
authors: Ribeiro
authors: F R F
authors: FR
authors: Nascimento
authors: S R F
authors: SR
authors: Pereira
mesh_terms: D000818:Animals; D000981:Antiprotozoal Agents / Q000737:chemistry / Q000633:toxicity*; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay; D029802:Croton / Q000737:chemistry*; D005260:Female; D006801:Humans; D007891:Leishmania / Q000187:drug effects*; D007962:Leukocytes / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D009152:Mutagenicity Tests; D010936:Plant Extracts / Q000737:chemistry / Q000633:toxicity*
chemical_list: D000981:Antiprotozoal Agents; D010936:Plant Extracts