title: Aberrant expression of signaling proteins in essential thrombocythemia.
abstract: Dysregulated expression of signaling proteins may contribute to the pathophysiology of essential thrombocythemia (ET). This study aimed to characterize protein expression in ET and to correlate the dysregulated proteins with phenotypes and prognosis of ET patients. The expression of 128 proteins in peripheral blood neutrophils from 74 ET patients was assessed and compared with those from 29 healthy subjects and 35 polycythemia vera (PV) patients using protein pathway array. Fifteen proteins were differentially expressed between ET patients and normal controls. These dysregulated proteins were involved in the signaling pathways related with apoptosis and inflammation. Our results showed a significant overlap in protein expression between ET patients with JAK2V617F mutation and PV patients. In addition, nine proteins were associated with JAK2V617F mutation status in ET patients. Furthermore, estrogen receptor beta (ERβ) and Stat3 were independent risk factors for subsequent thrombosis during follow-up on multivariable analysis. Our study shows a broad dysregulation of signaling protein in ET patients, suggesting their roles in ET pathogenesis. The expression levels of ERβ and Stat3 could be promising predictors of subsequent thrombosis in ET patients.
authors: Department of Hematology, Xuan Wu Hospital, Capital Medical University, Beijing, 100053, China.
authors: Wuhan
authors: W
authors: Hui
authors: Fei
authors: F
authors: Ye
authors: Wei
authors: W
authors: Zhang
authors: Congyan
authors: C
authors: Liu
authors: Miao
authors: M
authors: Cui
authors: Wei
authors: W
authors: Li
authors: Juan
authors: J
authors: Xu
authors: David Y
authors: DY
authors: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D047629:Estrogen Receptor beta / Q000096:biosynthesis / Q000235:genetics*; D005260:Female; D005500:Follow-Up Studies; D005786:Gene Expression Regulation; D005820:Genetic Testing; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000096:biosynthesis / Q000235:genetics*; D053614:Janus Kinase 2 / Q000096:biosynthesis / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009154:Mutation / Q000235:genetics*; D011087:Polycythemia Vera / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D012307:Risk Factors; D050796:STAT3 Transcription Factor / Q000096:biosynthesis / Q000235:genetics*; D013920:Thrombocythemia, Essential / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D013927:Thrombosis / Q000235:genetics / Q000378:metabolism; D055815:Young Adult
chemical_list: D047629:Estrogen Receptor beta; D047908:Intracellular Signaling Peptides and Proteins; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; C507924:JAK2 protein, human; D053614:Janus Kinase 2