title: The biotransformation of oleuropein in rats.
abstract: A highly sensitive and specific LC-MS/MS method was developed to investigate the in vivo bio-transformation of oleuropein in rat. Rat feces and urine samples collected after oral administration were determined by liquid chromatography coupled to tandem mass spectrometry with electrospray ionization in the negative-ion mode. The assay procedure involves a simple liquid-liquid extraction of parent oleuropein and the metabolite from rat feces and urine with ethyl acetate. Chromatographic separation was operated with 0.1% formic acid aqueous and methanol in gradient program at a flow rate of 0.50 mL/min on an RP-C18 column with a total run time of 31 min. This method was successfully applied to simultaneous determination of oleuropein and its metabolites in rat feces and urine. De-glucosylation, hydrolysis, oxygenation and methylation were found to comprise the major metabolic pathway of oleuropein in rat gastrointestinal tract and three metabolites were absorbed into the blood circulatory system within 24 h after oral administration.
authors: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, 151 Malianwa North Road, Haidian District, Beijing 100193, PR China.
authors: Peiyan
authors: P
authors: Lin
authors: Weidong
authors: W
authors: Qian
authors: Xiaoying
authors: X
authors: Wang
authors: Li
authors: L
authors: Cao
authors: Shaofen
authors: S
authors: Li
authors: Tianxiu
authors: T
authors: Qian
mesh_terms: D000818:Animals; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005243:Feces / Q000737:chemistry; D057888:Iridoid Glucosides; D039823:Iridoids; D059625:Liquid-Liquid Extraction; D008297:Male; D053858:Metabolic Networks and Pathways; D011714:Pyrans / Q000032:analysis / Q000737:chemistry / Q000493:pharmacokinetics* / Q000652:urine; D051381:Rats; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D057888:Iridoid Glucosides; D039823:Iridoids; D011714:Pyrans; C002769:oleuropein
keywords: LC-MS/MS; feces; in vivo metabolism; oleuropein; urine