title: Effect of low-level laser therapy on the release of interleukin-6 and basic fibroblast growth factor from cultured human skin fibroblasts in normal and high glucose mediums.
abstract: BACKGROUND
  This study evaluated the effects of low-level laser therapy (LLLT) on human skin fibroblasts (HSFs) that have been cultured in high glucose concentration media.
  
  
  METHODS
  HSFs were cultured under physiological glucose condition medium, and then cultured in high glucose concentration medium (15 mM/L) for 1 or 2 weeks prior to LLLT. Experimental HSFs were irradiated with three energy densities (0.5, 1, and 2 J/cm(2)) once daily for three consecutive days. Release of interleukin-6 (IL-6) and basic fibroblast growth factor (bFGF) was evaluated using the enzyme-linked immunosorbent assay (ELISA) method.
  
  
  RESULTS
  Statistical analysis showed three doses of 0.5 (p = 0.049), 1 (p = 0.027), and 2 J/cm(2) (p = 0.004) stimulated the release of IL-6 in HSFs cultured in high glucose concentration medium compared with that of non-irradiated HSFs that were cultured in the same medium. LLLT with 2 J/cm(2) induced the release of bFGF from HSFs cultured in high glucose concentration medium for 1 or 2 weeks (both p = 0.04).
  
  
  CONCLUSIONS
  Our study showed that LLLT stimulated the release of IL-6 and bFGF from HSFs cultured in high glucose concentration medium. LLLT was more effective in releasing IL-6 and bFGF while HSFs which were cultured in physiologic glucose concentration medium during laser irradiation.
authors: Oral and Maxillofacial Surgery Department, Dental Faculty, Shahid Beheshti University of Medical Sciences , Tehran , Iran.
authors: Mohammad
authors: M
authors: Esmaeelinejad
authors: Mohammad
authors: M
authors: Bayat
mesh_terms: D002460:Cell Line; D003470:Culture Media / Q000737:chemistry; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D007378:Interleukins / Q000378:metabolism*; D028022:Low-Level Light Therapy; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects
chemical_list: D003470:Culture Media; D007378:Interleukins; D016222:Fibroblast Growth Factor 2; D005947:Glucose