title: Flotillin2 expression correlates with HER2 levels and poor prognosis in gastric cancer.
abstract: OBJECTIVE
  Flotillin gene is known as a tumor promoter or suppressor, depending on the tumor type or tumor stage. We aimed to investigate the clinical significance of flotillin2 protein expression in gastric cancer.
  
  
  METHODS
  We examined flotillin2 and erbB2 levels in tissue microarray of 282 gastric cancer samples and analyzed the association between flotillin2 levels, clinicopathologic factors and prognosis. The regulation of erbB2 by flotillin2 was examined with flotillin2 siRNA-transfected gastric cancer cells.
  
  
  RESULTS
  Flotillin2 partially co-localized with erbB2 at the plasma membrane as detected by confocal microscopy, levels of erbB2 were reduced after flotillin knockdown in SGC-7901 cancer cells, and the expression of flotillin2 was positively correlated with that of erbB2. In non-neoplastic gastric mucosa, flotillin2 was not expressed in the epithelial compartment. In gastric cancer, positive staining of flotillin2 was shown in 129 (45.7%) of 282 cases, also, it was significantly associated with a Lauren grade, histologic type, lymphovascular invasion and tumor location. Moreover, survival analysis showed that flotillin2 expression was an independent prognostic factor of poor survival (p<0.001).
  
  
  CONCLUSIONS
  These results indicate that a positive correlation exists between flotillin2 and erbB2 expression levels, flotillin2 maybe involved in the stabilization of erbB2 at the plasma membrane, flotillin2 is significantly correlated with cancer progression and poor prognosis in gastric cancer.
authors: Department of Surgical Oncology, Department of General Surgery, The First Hospital of China Medical University, Shenyang, China.
authors: Zhi
authors: Z
authors: Zhu
authors: Jinou
authors: J
authors: Wang
authors: Zhe
authors: Z
authors: Sun
authors: Xuren
authors: X
authors: Sun
authors: Zhenning
authors: Z
authors: Wang
authors: Huimian
authors: H
authors: Xu
mesh_terms: D000368:Aged; D063646:Carcinogenesis; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D011379:Prognosis; D021381:Protein Transport; D059748:Proteolysis; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics* / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000175:diagnosis* / Q000235:genetics / Q000378:metabolism* / Q000473:pathology
chemical_list: D008565:Membrane Proteins; D018719:Erb-b2 Receptor Tyrosine Kinases; C106354:flotillins; C508053:ERBB2 protein, human
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