title: Membrane-tethered ligands: tools for cell-autonomous pharmacological manipulation of biological circuits.
abstract: Detection of secreted signaling molecules by cognate cell surface receptors is a major intercellular communication pathway in cellular circuits that control biological processes. Understanding the biological significance of these connections would allow us to understand how cellular circuits operate as a whole. Membrane-tethered ligands are recombinant transgenes with structural modules that allow them to act on cell-surface receptors and ion channel subtypes with pharmacological specificity in a cell-autonomous manner. Membrane-tethered ligands have been successful in the specific manipulation of ion channels as well as G-protein-coupled receptors, and, in combination with cell-specific promoters, such manipulations have been restricted to genetically defined subpopulations within cellular circuits in vivo to induce specific phenotypes controlled by those circuits. These studies establish the membrane-tethering approach as a generally applicable method for dissecting neural and physiological circuits.
authors: Department of Cellular and Molecular Physiology and Molecular Physiology, Yale School of Medicine, New Haven, Connecticut, USA.
authors: Charles
authors: C
authors: Choi
authors: Michael N
authors: MN
authors: Nitabach
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D058851:GPI-Linked Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007473:Ion Channels / Q000378:metabolism*; D008024:Ligands; D009498:Neurotoxins / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D011956:Receptors, Cell Surface / Q000378:metabolism*; D011978:Receptors, Nicotinic / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D019076:Transgenes
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D058851:GPI-Linked Proteins; D007473:Ion Channels; C485626:LYNX1 protein, human; D008024:Ligands; D009498:Neurotoxins; D011956:Receptors, Cell Surface; D011978:Receptors, Nicotinic; D011993:Recombinant Fusion Proteins
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