title: Effectiveness of hybridized nano- and microstructure biodegradable, biocompatible, collagen-based, three-dimensional bioimplants in repair of a large tendon-defect model in rabbits.
abstract: This study was designed to investigate the effectiveness of hybridized, three-dimensional (3D) collagen implants in repair of experimentally-induced tendon defects in rabbits. Seventy-five mature New Zealand albino rabbits were divided into treated (n = 50) and control (n = 20) groups. The left Achilles tendon was completely transected and 2 cm excised. In treated animals defects were filled with hybridized collagen implants and repaired with sutures. In control rabbits tendon defects were sutured similarly but the gap was left untreated. Changes in injured and normal contralateral tendons were assessed weekly by ultrasonography. Among the treated animals, small pilot groups were euthanized at 5, 10, 15, 20, 30, 40 (n = 5 at each time interval) and the remainder (n = 20) at 60 days post-injury. All control animals were euthanized at 60 days. Tendon lesions of all animals were examined morphologically and histologically immediately after death. Those of the experimental groups (n = 20 for each) were examined for gross pathological, histopathological and ultrastructural changes together with dry matter content at 60 days post-injury, as were the normal, contralateral tendons of both groups. In comparison with healing lesions of control animals, the treated tendons showed greater numbers of mature tenoblasts and tenocytes, minimal peritendinous adhesions and oedema, together with greater echogenicity, homogeneity and fibril alignment. Fewer chronic inflammatory cells were present in treated than control tendons. Hybridized collagen implants acted as scaffolds for tenoblasts and longitudinally-orientated newly-formed collagen fibrils, which encouraged tendon repair with homogeneous, well-organized highly aligned scar tissue that was histologically and ultrastructurally more mature than in untreated controls. Copyright © 2016 John Wiley & Sons, Ltd.
authors: Division of Surgery and Radiology, Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Iran.
authors: Ali
authors: A
authors: Moshiri
authors: Department of Pathology, School of Veterinary Medicine, Shiraz University, Iran.
authors: Ahmad
authors: A
authors: Oryan
authors: Division of Surgery and Radiology, Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Iran.
authors: Abdulhamid
authors: A
authors: Meimandi-Parizi
authors: Centre for Comparative and Clinical Anatomy, School of Veterinary Science, Southwell Street, Bristol, UK.
authors: Ian A
authors: IA
authors: Silver
authors: Centre for Stem Cell Research and Transgenic Animals, Shiraz University of Medical Sciences, Iran.
authors: Nader
authors: N
authors: Tanideh
authors: Division of Surgery and Radiology, Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Iran.
authors: Navid
authors: N
authors: Golestani
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001705:Bioprosthesis; D049329:Nanostructures / Q000737:chemistry*; D011817:Rabbits; D013708:Tendon Injuries / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D013710:Tendons / Q000378:metabolism* / Q000473:pathology; D054457:Tissue Scaffolds / Q000737:chemistry*
keywords: biocompatible; biodegradable; bioimplant; collagen; healing; rabbits; tendon