title: No effect of antifungal compounds on functional properties of human antifungal T-helper type 1 cells.
abstract: Despite the availability of new antifungal compounds, invasive fungal disease is associated with a high mortality in hematopoietic stem cell transplant (HSCT) recipients. A growing body of evidence suggests that T lymphocytes from the T-helper type 1 (TH 1) play an important role in the antifungal host defense, and preliminary data indicate a potential benefit of infusing donor-derived antifungal TH 1 cells to HSCT patients suffering from invasive fungal disease. Unfortunately, it is unclear to date whether the function of these cells is affected by concomitantly administered antifungal agents. We therefore analyzed the effects of various concentrations of commonly used antifungal compounds such as amphotericin B, caspofungin, fluconazole, voriconazole, and posaconazole on the functional properties of cultivated human antifungal TH 1 cells. None of the antifungal compounds tested significantly influenced the secretion of interferon-γ and tumor necrosis factor-α, and only posaconazole at high concentrations slightly decreased proliferation of antifungal TH 1 cells. Our data indicate that the antifungal agents tested do not significantly affect the functional properties of antifungal TH 1 cells and can therefore be concomitantly administered.
authors: Pediatric Hematology and Oncology, Johann Wolfgang Goethe University, Frankfurt, Germany.
authors: L
authors: L
authors: Tramsen
authors: S
authors: S
authors: Schmidt
authors: U
authors: U
authors: Koehl
authors: S
authors: S
authors: Huenecke
authors: J-P
authors: JP
authors: Latgé
authors: F
authors: F
authors: Roeger
authors: R
authors: R
authors: Schubert
authors: T
authors: T
authors: Klingebiel
authors: T
authors: T
authors: Lehrnbecher
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology* / Q000627:therapeutic use; D002478:Cells, Cultured; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects; D006801:Humans; D007371:Interferon-gamma / Q000187:drug effects / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects; D009181:Mycoses / Q000276:immunology / Q000517:prevention & control; D018417:Th1 Cells / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D014230:Triazoles / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000187:drug effects / Q000378:metabolism
chemical_list: D000935:Antifungal Agents; D014230:Triazoles; D014409:Tumor Necrosis Factor-alpha; C101425:posaconazole; D007371:Interferon-gamma
keywords: amphotericin B; antifungal T cell; echinocandin; triazole