title: Cytoskeleton and regulation of mitochondrial function: the role of beta-tubulin II.
abstract: The control of mitochondrial function is a cardinal issue in the field of cardiac bioenergetics, and the analysis of mitochondrial regulations is central to basic research and in the diagnosis of many diseases. Interaction between cytoskeletal proteins and mitochondria can actively participate in mitochondrial regulation. Potential candidates for the key roles in this regulation are the cytoskeletal proteins plectin and tubulin. Analysis of cardiac cells has revealed regular arrangement of β-tubulin II, fully co-localized with mitochondria. β-Tubulin IV demonstrated a characteristic staining of branched network, β-tubulin III was matched with Z-lines, and β-tubulin I was diffusely spotted and fragmentary polymerized. In contrast, HL-1 cells were characterized by the complete absence of β-tubulin II. Comparative analysis of cardiomyocytes and HL-1 cells revealed a dramatic difference in the mechanisms of mitochondrial regulation. In the heart, colocalization of β-tubulin isotype II with mitochondria suggests that it can participate in the coupling of ATP-ADP translocase (ANT), mitochondrial creatine kinase (MtCK), and VDAC (ANT-MtCK-VDAC). This mitochondrial supercomplex is responsible for the efficient intracellular energy transfer via the phosphocreatine pathway. Existing data suggest that cytoskeletal proteins may control the VDAC, contributing to maintenance of mitochondrial and cellular physiology.
authors: Cardiac Surgery Research Laboratory, Department of Cardiac Surgery, Innsbruck Medical University Innsbruck, Tirol, Austria.
authors: Andrey V
authors: AV
authors: Kuznetsov
authors: Sabzali
authors: S
authors: Javadov
authors: Rita
authors: R
authors: Guzun
authors: Michael
authors: M
authors: Grimm
authors: Valdur
authors: V
authors: Saks
keywords: HL-1 cells; VDAC; beta tubulin isotypes; cardiomyocytes; confocal microscopy; creatine kinase; mitochondria-cytoskeleton interactions; mitochondrial regulation
references: Biochem J. 2008 Feb 15;410(1):141-6
references: 17983357
references: Biochim Biophys Acta. 2011 Apr;1807(4):458-69
references: 21296049
references: Am J Physiol Cell Physiol. 2003 Feb;284(2):C285-93
references: 12529248
references: Am J Physiol. 1990 Mar;258(3 Pt 1):C377-89
references: 2138418
references: J Mol Cell Cardiol. 2012 Feb;52(2):437-47
references: 21846472
references: Am J Physiol. 1991 Oct;261(4 Suppl):30-8
references: 1928451
references: Circ Res. 2001 Jul 20;89(2):153-9
references: 11463722
references: J Appl Physiol (1985). 2002 Jun;92(6):2429-38
references: 12015357
references: Eur J Biochem. 1996 Nov 1;241(3):909-15
references: 8944782
references: Mol Cell Biochem. 1998 Jul;184(1-2):101-5
references: 9746315
references: Biochim Biophys Acta. 1991 Jul 8;1074(2):302-11
references: 2065083
references: Histochemistry. 1990;95(1):1-10
references: 2286530
references: Cell. 1982 Dec;31(3 Pt 2):681-92
references: 6186387
references: J Mol Cell Cardiol. 2002 Oct;34(10):1259-71
references: 12392982
references: Proc Natl Acad Sci U S A. 2008 Dec 2;105(48):18746-51
references: 19033201
references: Biochim Biophys Acta. 1997 Nov 10;1322(1):41-59
references: 9398078
references: Exp Physiol. 2003 Jan;88(1):175-90
references: 12525866
references: Biophys J. 2003 May;84(5):3436-56
references: 12719270
references: J Biol Chem. 1995 Aug 25;270(34):19921-9
references: 7650007
references: Exerc Sport Sci Rev. 2002 Jul;30(3):129-37
references: 12150572
references: J Cell Biol. 2008 May 19;181(4):667-81
references: 18490514
references: J Cell Biol. 2000 Sep 18;150(6):1283-98
references: 10995435
references: Trends Cardiovasc Med. 2002 Nov;12(8):339-48
references: 12536120
references: Nat Protoc. 2008;3(6):965-76
references: 18536644
references: Biochemistry. 2012 Apr 10;51(14):2959-73
references: 22443365
references: Biochim Biophys Acta. 2010 Jun-Jul;1797(6-7):678-97
references: 20096261
references: J Mol Cell Cardiol. 2001 May;33(5):947-56
references: 11343417
references: Mol Cell Biochem. 1997 Sep;174(1-2):79-85
references: 9309669
references: J Biol Chem. 1998 Nov 27;273(48):31829-36
references: 9822650
references: Biochem Biophys Res Commun. 1989 Dec 15;165(2):748-57
references: 2557020
references: J Physiol. 2006 Dec 15;577(Pt 3):769-77
references: 17008367
references: Biochem Biophys Res Commun. 1995 Mar 28;208(3):919-26
references: 7702621
references: J Biol Chem. 2000 Mar 10;275(10):6937-44
references: 10702255
references: Am J Physiol Regul Integr Comp Physiol. 2006 May;290(5):R1271-5
references: 16357098
references: J Exp Biol. 2003 Jun;206(Pt 12):2059-72
references: 12756288
references: Mol Cell Biochem. 2000 May;208(1-2):119-28
references: 10939635
references: Biochem J. 2001 Jun 1;356(Pt 2):643-57
references: 11368796
references: J Biol Chem. 1985 Jun 25;260(12):7757-64
references: 3997893
references: Am J Physiol Cell Physiol. 2005 Mar;288(3):C757-67
references: 15496480
references: Biokhimiia. 1990 Nov;55(11):1944-57
references: 2085614
references: J Physiol. 2002 Aug 15;543(Pt 1):191-200
references: 12181291
references: Annu Rev Physiol. 1979;41:485-506
references: 373602
references: J Cell Biol. 1998 Mar 9;140(5):1091-9
references: 9490722
references: Biochem Biophys Res Commun. 1995 Aug 4;213(1):138-46
references: 7639727
references: Biochim Biophys Acta. 2006 Dec;1757(12):1597-606
references: 17084805
references: Biochim Biophys Acta. 2006 May-Jun;1757(5-6):692-9
references: 16729962
references: Biochim Biophys Acta. 2001 Jun 1;1505(2-3):258-70
references: 11334790
references: J Cell Biol. 2008 Jun 16;181(6):903-11
references: 18541706
references: Mol Cell Biochem. 1998 Jun;183(1-2):87-96
references: 9655182
references: J Bioenerg Biomembr. 2008 Jun;40(3):163-70
references: 18654841
references: J Biol Chem. 2002 Sep 13;277(37):33664-9
references: 12087096
references: J Mol Cell Cardiol. 2012 Feb;52(2):419-36
references: 21816155
references: Biochim Biophys Acta. 1993 Sep 13;1144(2):134-48
references: 8396441
references: J Cell Sci. 2005 Dec 1;118(Pt 23):5411-9
references: 16306220
references: J Muscle Res Cell Motil. 2001;22(2):141-7
references: 11519737
references: J Physiol. 2006 Mar 1;571(Pt 2):253-73
references: 16410283