title: Low glucose promotes CD133mAb-elicited cell death via inhibition of autophagy in hepatocarcinoma cells.
abstract: CD133 on cancer stem cells is a potential therapeutic target. In this study, CD133 antibody (CD133mAb) treatment resulted in cell death in hepatoma LM3, HepG2, Hep3B and Huh-7 cells, especially under low glucose condition. The treatment also inhibited formation of spheroids, colonies, and xenograft tumors. Ectopic CD133 enabled hepatocyte L02 to be suppressed by CD133mAb and increased spheroid formation. CD133mAb caused cell death in primary HCC cells and sensitized them to Doxorubicin and Cisplatin. The antibody effect was attributed to suppressing autophagy and promoting necrotic cell death. Therefore, targeting CD133 under low glucose condition is a potential therapeutic approach for hepatocarcinomas.
authors: International Cooperation Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Institute/Hospital, The Second Military Medical University, 225 Changhai Road, Shanghai 200438, China.
authors: Haiyang
authors: H
authors: Chen
authors: Zaili
authors: Z
authors: Luo
authors: Wen
authors: W
authors: Sun
authors: Cunzhen
authors: C
authors: Zhang
authors: Huanlin
authors: H
authors: Sun
authors: Nanjie
authors: N
authors: Zhao
authors: Jin
authors: J
authors: Ding
authors: Mengchao
authors: M
authors: Wu
authors: Zhong
authors: Z
authors: Li
authors: Hongyang
authors: H
authors: Wang
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D015703:Antigens, CD; D017209:Apoptosis; D001343:Autophagy; D006528:Carcinoma, Hepatocellular / Q000276:immunology / Q000378:metabolism*; D002452:Cell Count; D016923:Cell Death; D045744:Cell Line, Tumor; D003094:Collagen / Q000737:chemistry; D004338:Drug Combinations; D005947:Glucose / Q000494:pharmacology*; D006023:Glycoproteins; D022781:Hepatocytes / Q000166:cytology; D006801:Humans; D007797:Laminin / Q000737:chemistry; D008113:Liver Neoplasms / Q000276:immunology / Q000378:metabolism*; D008297:Male; D051379:Mice; D008819:Mice, Nude; D009336:Necrosis; D009368:Neoplasm Transplantation; D010455:Peptides; D011509:Proteoglycans / Q000737:chemistry
chemical_list: D000071916:AC133 Antigen; D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D004338:Drug Combinations; D006023:Glycoproteins; D007797:Laminin; C000606023:PROM1 protein, human; D010455:Peptides; C000606024:Prom1 protein, mouse; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen; D005947:Glucose