title: Association between IL8 haplotypes and pathogen levels in chronic periodontitis.
abstract: Chronic periodontitis (CP) is considered to be a multifactorial disease influenced by microbial and genetic factors. The aim of the present study was to investigate whether the genetic susceptibility to CP in individuals with the IL8 ATC/TTC haplotype is associated with subgingival levels of periodontopathogens. Sixty-five individuals, grouped according to the presence (n = 28) or absence (n = 37) of the IL8 haplotype, were evaluated. After clinical periodontal evaluation, each group was subdivided according to the presence (CP) or absence (H) of periodontitis. Four subgingival samples were obtained from CP and two samples per subject from H patients. The levels and proportions of Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola were analyzed using quantitative real-time polymerase chain reaction (q-PCR). No differences were found in the proportion of periodontopathogenic bacteria between groups with the presence or absence of the IL8 haplotype. However, in the CP groups, the levels of periodontopathogens were significantly higher in the individuals without the IL8 haplotype than in the individuals with the IL8 haplotype. These results suggest that periodontal destruction may occur in patients who are considered to be genetically susceptible to CP with a lower microbial challenge because of the presence of the IL8 ATC/TTC haplotype than in patients without this haplotype.
authors: Department of Oral Diagnosis and Surgery, School of Dentistry at Araraquara, UNESP, Universidade Estadual Paulista, Araraquara, SP, Brazil.
authors: L S
authors: LS
authors: Finoti
authors: S C T
authors: SC
authors: Corbi
authors: G
authors: G
authors: Anovazzi
authors: S R L
authors: SR
authors: Teixeira
authors: J P
authors: JP
authors: Steffens
authors: R
authors: R
authors: Secolin
authors: Y J
authors: YJ
authors: Kim
authors: S R P
authors: SR
authors: Orrico
authors: J A
authors: JA
authors: Cirelli
authors: M P A
authors: MP
authors: Mayer
authors: R M
authors: RM
authors: Scarel-Caminaga
mesh_terms: D000328:Adult; D058491:Bacterial Load; D041963:Bacteroidetes / Q000276:immunology / Q000302:isolation & purification*; D055113:Chronic Periodontitis / Q000276:immunology* / Q000382:microbiology; D005260:Female; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D016966:Porphyromonas gingivalis / Q000276:immunology / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction; D048373:Treponema denticola / Q000276:immunology / Q000302:isolation & purification*
chemical_list: C508606:CXCL8 protein, human; D016209:Interleukin-8
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