title: Rational design of orthogonal libraries of protein coding genes.
abstract: Array-based oligonucleotide synthesis technologies provide access to thousands of custom-designed sequence variants at low cost. Large-scale synthesis and high-throughput assays have become valuable experimental tools to study in detail the interplay between sequence and function. We have developed a methodology and corresponding algorithms for the design of diverse protein coding gene libraries, to exploit the potential of multiplex synthesis and help elucidate the effects of codon utilization and other factors in gene expression. Using our algorithm, we have computationally designed gene libraries with hundreds to thousands of orthogonal codon usage variants, uniformly exploring the design space of codon utilization, while demanding only a small fraction of the synthesis cost that would be required if these variants were synthesized independently.
authors: National Institute for Mathematical and Biological Synthesis, University of Tennessee, Knoxville, TN 37996, United States.
authors: Daniel
authors: D
authors: Ryan
authors: Dimitris
authors: D
authors: Papamichail
mesh_terms: D000465:Algorithms; D001483:Base Sequence; D015723:Gene Library; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics*; D015202:Protein Engineering / Q000379:methods*; D011506:Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D011506:Proteins