title: Heparanase-1 activities in the development of laser induced choroidal neovascularization.
abstract: OBJECTIVE
  To investigate the role of heparanase-1 in laser-induced choroidal neovascularization (CNV).
  
  
  METHODS
  Experimental CNV was induced by krypton laser photocoagulation in 15 male Brown Norway rats. Fundus fluorescein angiography and histopathological examination were performed in observing the CNV development. The expression and distribution of heparanase-1 protein in the laser lesions were determined by immunohistochemistry and western blotting analysis.
  
  
  RESULTS
  The success rate of laser induced CNV was approximately 75% on 3-4 weeks after laser photocoagulation. The protein levels of heparanase-1 increased significantly in the retina-choroidal complex of CNV models when compared to normal rat eyes (P<0.01). Immunostaining confirmed strong heparanase-1 expressions in all laser lesions, and it displayed to be highest at the newly formed blood vessels within the fibrovascular complex in the subretinal space.
  
  
  CONCLUSIONS
  Heparanase-1 is closely involved in the development of laser induced CNV.
authors: Department of Ophthalmology, the First Affiliated Hospital of General Hospital of People's Liberation Army, Beijing 100048, China.
authors: Wei-Qiang
authors: WQ
authors: Tang
authors: Bao-Ke
authors: BK
authors: Hou
keywords: choroidal neovascularization; heparanase-1; immunohistochemistry; western blotting
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