title: Prognostic value of human papillomavirus types 16 and 18 DNA physical status in cervical intraepithelial neoplasia.
abstract: The aim of this work was to assess the value of the physical status of human papillomavirus (HPV) DNA as a disease marker for cervical cancer development in a set of 248 DNA samples previously genotyped as HPV 16 or 18, by calculating the E2/E6 ratio through real-time PCR. There was a significant difference in integration status according to disease grade for both genotypes (p <0.001). Furthermore, especially for HPV 18, determining the DNA physical status could be a useful biomarker in predicting cervical cancer risk development, with a lower E2/E6 ratio clinically associated with the development of a precancerous lesion.
authors: National Reference Laboratory of STI for Human Papillomavirus and Genital Herpes Virus, Department of Infectious Diseases, National Institute of Health, Lisbon, Portugal.
authors: A
authors: A
authors: Gradíssimo Oliveira
authors: C
authors: C
authors: Delgado
authors: N
authors: N
authors: Verdasca
authors: A
authors: A
authors: Pista
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D015331:Cohort Studies; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005819:Genetic Markers / Q000235:genetics; D052162:Human papillomavirus 16 / Q000235:genetics / Q000302:isolation & purification*; D052161:Human papillomavirus 18 / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000473:pathology* / Q000821:virology*; D011379:Prognosis; D018570:Risk Assessment; D002583:Uterine Cervical Neoplasms / Q000473:pathology* / Q000821:virology*; D055815:Young Adult; D002578:Uterine Cervical Dysplasia / Q000473:pathology* / Q000821:virology*
chemical_list: D004279:DNA, Viral; D005819:Genetic Markers
keywords: Cervical cancer; human papillomavirus 18; molecular marker; physical status; risk assessment