title: [Molecular epidemiological study on HIV/AIDS under the follow-up program in Zhejiang province in 2009].
abstract: OBJECTIVE
  To analyze the molecular epidemiological characteristics on HIV infectors/AIDS patients (HIV/AIDS) under a follow-up program in Zhejiang province in 2009.
  
  
  METHODS
  303 cases were randomly sampled. Information on the cases was collected and followed by genomic DNA extraction. Gag gene fragments were amplified by nested PCR, followed by sequencing and bio-informatic analysis.
  
  
  RESULTS
  The rate of success for sequence acquisition was 74.3% (225/303). Distributions of HIV subtypes were as follows: CRF01_AE (58.7%), CRF07_BC (13.8%), CRF08_BC (9.8%), B' (15.1%), C (1.8%), G (0.4%) and unassigned BC (unique recombinant form 0.4%).
  
  
  RESULTS
  from the HIV BLAST analysis showed that the sources of strains with the highest homology involved in 10 provinces/municipalities (Liaoning, Guangxi, Yunnan, Henan, etc.) and five other countries (Thailand, Vietnam, India, South Africa and Libya). The CRF01_AE phylogenetic tree was divided into four clusters. The sequences of HIV/AIDS with homosexual transmission showed a gather in cluster 1, and mix with those infected through heterosexual contact.
  
  
  CONCLUSIONS
  Circulating recombinant forms of HIV seemed to play a dominant role in Zhejiang province. Unique recombinant form and new subtype of HIV were found. People living with HIV under homosexual transmission and heterosexual transmission had a trend of interwoven with each other. Increase of both the diversity and complexity of HIV strains were also noticed in Zhejiang province.
authors: Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China.
authors: Jia-feng
authors: JF
authors: Zhang
authors: Xiao-hong
authors: XH
authors: Pan
authors: Xiao-bei
authors: XB
authors: Ding
authors: Lin
authors: L
authors: Chen
authors: Zhi-hong
authors: ZH
authors: Guo
authors: Yun
authors: Y
authors: Xu
authors: Jing-jing
authors: JJ
authors: Huang
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000453:epidemiology*; D000328:Adult; D002681:China / Q000453:epidemiology; D005260:Female; D006678:HIV / Q000235:genetics*; D015658:HIV Infections / Q000453:epidemiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D010802:Phylogeny