title: [Study on the association between genetic variation of gag gene and HLA restricted CTL response in HIV-1 B' infected Chinese people].
abstract: OBJECTIVE
  To identify the genetic variation of Gag gene in HIV-1 B' subtype infected Chinese people and to evaluate the association between the positive selection sites and HLA restricted CTL response.
  
  
  METHODS
  We downloaded all HIV-1 B' subtype gag gene sampled from China from the LANL HIV database. The sequences were grouped by years of collection and the mean intra-subtype diversity and divergence were examined by MEGA 5.0 software. The sites under positive selection were estimated by using Hyphy software. Then analyzed the association between positively selected sites and HLA restricted CTL epitopes.
  
  
  RESULTS
  A set of 122 B' subtype HIV-1 gag sequences were collected from Henan, Hubei, Liaoning and Yunnan provinces, grouped by sampling year as < 2001, 2001 - 2003, 2004 - 2006 and 2007 - 2010. The mean intra-subtype diversity and divergence increased over time (r(1) = 0.497, P1 < 0.001; r(2) = 0.593, P2 < 0.001). This analysis revealed 34 positively selected sites and 97.1% of these sites were located within 47 known CTL epitopes. The most frequent HLA was HLA-A*02 (21.6%) followed by A*11(9.8%), B*35(9.8%) and B*57(5.9%). The percentages of HLA-A/B/C limited CTL epitopes were 43.1%, 49.0% and 7.9%, respectively.
  
  
  CONCLUSIONS
  The mean intra-subtype diversity and divergence of B' subtype gag sequences increased over time. B' subtype HIV-1 virus has accumulated adaptations to HLA restricted CTL responses in HIV-1 infected Chinese.
authors: Key Laboratory of AIDS Immunology of Ministry of Health, Department of Laboratory Medicine, No.1 Hospital of China Medical University, Shenyang 110001, China.
authors: Hui
authors: H
authors: Zhang
authors: Xiao-xu
authors: XX
authors: Han
authors: Di
authors: D
authors: Dai
authors: Bin
authors: B
authors: Zhao
authors: Ming-hui
authors: MH
authors: An
authors: Hong
authors: H
authors: Shang
mesh_terms: D044466:Asian People / Q000235:genetics; D002681:China; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D014644:Genetic Variation; D006679:HIV Seropositivity; D006801:Humans; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology*; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000235:genetics* / Q000276:immunology*
chemical_list: D018984:Epitopes, T-Lymphocyte; D054301:gag Gene Products, Human Immunodeficiency Virus