title: CXCL12-γ expression is inhibited in neuroinflammation.
abstract: CXCL12 plays a protective role in CNS autoimmunity. Expression of CXCL12-γ, which has distinct structural and functional properties than the other isoforms of CXCL12, was determined in spinal cords of rats immunized to develop experimental autoimmune encephalomyelitis (EAE). CNS expression of CXCL12-γ was markedly lower in EAE-prone Dark Agouti rats than in EAE-resistant Albino Oxford rats, both in spinal cord homogenates and micro-blood vessels isolated from spinal cords. Inhibition of nitric oxide (NO) synthesis in DA rats upregulated, while donation of NO in AO rats downregulated CNS expression of CXCL12-γ. NO inhibited CXCL12-γ expression in astrocytes in vitro. A splice variant of CXCL12-γ which migrates into nucleolus was not detected in spinal cord or astrocytes. Thus, CXCL12-γ is expressed in the CNS after EAE induction, but its expression is markedly suppressed in spinal cord affected with full blown inflammation. NO is an important regulator of CXCL12-γ expression in neuroinflammation.
authors: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Serbia.
authors: Gordana
authors: G
authors: Timotijević
authors: Filip
authors: F
authors: Petković
authors: Jana
authors: J
authors: Blaževski
authors: Miljana
authors: M
authors: Momčilović
authors: Marija
authors: M
authors: Mostarica Stojković
authors: Djordje
authors: D
authors: Miljković
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D054377:Chemokine CXCL12 / Q000235:genetics / Q000378:metabolism*; D016207:Cytokines / Q000494:pharmacology; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000139:chemically induced / Q000150:complications* / Q000473:pathology; D005260:Female; D005620:Freund's Adjuvant / Q000633:toxicity; D007249:Inflammation / Q000209:etiology* / Q000473:pathology*; D008297:Male; D019001:Nitric Oxide Synthase / Q000378:metabolism; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D013116:Spinal Cord / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors; D015854:Up-Regulation
chemical_list: D054377:Chemokine CXCL12; D016207:Cytokines; D005620:Freund's Adjuvant; D019001:Nitric Oxide Synthase