title: A convenient method for selective detection of 5-hydroxymethylcytosine and 5-formylcytosine sites in DNA sequences.
abstract: Treatment of DNA containing 5-formylcytosine with hot piperidine produced a cleaved band at the position of 5-formylcytosine in DNA sequences. After oxidation with KRuO4, 5-hydroxymethylcytosine could also be detected using the same method. Using our strategy, we could detect 5-hydroxymethylcytosine and 5-formylcytosine respectively.
authors: College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Hubei, Wuhan, 430072, P. R. of China.
authors: Wuxiang
authors: W
authors: Mao
authors: Jianlin
authors: J
authors: Hu
authors: Tingting
authors: T
authors: Hong
authors: Xiwen
authors: X
authors: Xing
authors: Sen
authors: S
authors: Wang
authors: Xi
authors: X
authors: Chen
authors: Xiang
authors: X
authors: Zhou
mesh_terms: D044503:5-Methylcytosine / Q000031:analogs & derivatives; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D020650:Combinatorial Chemistry Techniques; D003596:Cytosine / Q000031:analogs & derivatives* / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D017422:Sequence Analysis, DNA
chemical_list: C560973:5-formylcytosine; C011865:5-hydroxymethylcytosine; D044503:5-Methylcytosine; D003596:Cytosine