title: Poly[LA-(Glc-Leu)] copolymer as a carrier for ocular delivery of ciprofloxacin: formulation, characterization and in vivo biocompatibility study.
abstract: BACKGROUND
  Ciprofloxacin hydrochloride-encapsulated poly[LA-(Glc-Leu)] copolymeric nanoparticles were prepared to provide sustained release and ameliorate shortcomings associated with eye drops.
  
  
  METHODS
  Poly[LA-(Glc-Leu)] copolymer was synthesized by ring opening copolymerization of l-lactide with a cyclodepsipetide. Ciprofloxacin hydrochloride-loaded polymeric nanoparticles were prepared by double emulsification-solvent evaporation technique with emphasis on optimization of different process and formulation variables.
  
  
  RESULTS
  Optimized formulation showed encapsulation efficiency of approximately 63.08 ± 5.88% and a positive zeta potential of +17.46 mV. Scanning electron microscope study revealed the spherical shape of the nanoparticles with a particle size 300-400 nm. In vitro drug-release studies showed sustained release for 24 h with biphasic release pattern. In vivo ocular tolerability study in rabbits demonstrated no signs of toxicity or irritation parallel to the results obtained with the marketed formulation.
  
  
  CONCLUSIONS
  Poly[LA-(Glc-Leu)] copolymer can be used as an efficient carrier for nanoparticle preparation of ciprofloxacin.
authors: Centre for Novel Drug Delivery Systems, Department of Pharmaceutical Sciences & Technology, Institute of Chemical Technology, University under Section 3 of UGC Act- 1956, Elite Status & Centre of Excellence - Govt. of Maharashtra, TEQIP Phase II Funded, Matunga, Mumbai-400019, India.
authors: Kunal P
authors: KP
authors: Pagar
authors: Pradeep R
authors: PR
authors: Vavia
mesh_terms: D060433:Administration, Ophthalmic; D000818:Animals; D000890:Anti-Infective Agents / Q000008:administration & dosage* / Q000633:toxicity; D002939:Ciprofloxacin / Q000008:administration & dosage* / Q000633:toxicity; D003692:Delayed-Action Preparations; D047630:Depsipeptides / Q000737:chemistry; D004146:Dioxanes / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems; D004655:Emulsions; D005260:Female; D008297:Male; D051379:Mice; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles; D010316:Particle Size; D011108:Polymers / Q000737:chemistry; D011817:Rabbits; D012997:Solvents / Q000737:chemistry; D018675:Toxicity Tests
chemical_list: D000890:Anti-Infective Agents; D003692:Delayed-Action Preparations; D047630:Depsipeptides; D004146:Dioxanes; D004337:Drug Carriers; D004655:Emulsions; D011108:Polymers; D012997:Solvents; C582228:poly(LA-(Glc-Leu)); D002939:Ciprofloxacin; C091880:dilactide