title: In vitro response to alkaline phosphatase coatings immobilized onto titanium implants using electrospray deposition or polydopamine-assisted deposition.
abstract: Immobilization of biomolecules onto implant surfaces is one of the most straightforward strategies to control the interaction between an implant and its biological environment. Recently, it was shown that the enzyme alkaline phosphatase (ALP) could be efficiently immobilized onto titanium implants in a single step using polydopamine. We hypothesized that such polydopamine-ALP coatings can enhance the early attachment of cells and increase mineralization. Therefore, the current study aimed at immobilization of ALP onto titanium by means of either one- or two-step polydopamine-assisted immobilization or electrospray deposition, the comparative characterization of these experimental substrates and subsequent cell behavioral analysis using primary osteoblast-like cells. Uncoated titanium and ALP-free polydopamine coatings served as controls. Despite significant ALP surface activity and lower water contact for angles ALP-containing surface modifications, only marginal effects on early cell behavior (i.e., cell spreading) and osteogenic differentiation (i.e., proliferation, differentiation and mineralization) were observed in comparison to uncoated titanium.
authors: Department of Biomaterials, Radboud University Nijmegen Medical Centre, 6500 HB, Nijmegen, the Netherlands.
authors: Arnold W G
authors: AW
authors: Nijhuis
authors: Jeroen J J P
authors: JJ
authors: van den Beucken
authors: John A
authors: JA
authors: Jansen
authors: Sander C G
authors: SC
authors: Leeuwenburgh
mesh_terms: D000469:Alkaline Phosphatase / Q000494:pharmacology*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002417:Cattle; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D004247:DNA / Q000378:metabolism; D004800:Enzymes, Immobilized / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D007211:Indoles / Q000737:chemistry*; D008297:Male; D008422:Materials Testing / Q000379:methods*; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D011108:Polymers / Q000737:chemistry*; D019736:Prostheses and Implants; D051381:Rats; D017208:Rats, Wistar; D014025:Titanium / Q000494:pharmacology*; D014867:Water / Q000737:chemistry
chemical_list: D020099:Coated Materials, Biocompatible; D004800:Enzymes, Immobilized; D007211:Indoles; D011108:Polymers; C568283:polydopamine; D014867:Water; D004247:DNA; D014025:Titanium; D000469:Alkaline Phosphatase; D002118:Calcium
keywords: alkaline phosphatase; enzyme immobilization; polydopamine; surface modification; titanium implants