title: [Effects of oleic acid on expression of adiponectin and its PPARgamma mechanism in 3T3-L1 adipocytes].
abstract: OBJECTIVE
  To explore the effects of oleic acid on the gene and protein expression of adiponectin and its PPARgamma mechanism in 3T3-l1 adipocytes.
  
  
  METHODS
  L1 adipocytes were incubated with different concentration (25, 50, 100, 200, 400 micromol/L) for 24h, and the mRNA expression of adiponectin and PPARgamma was determined by real-time PCR (relative quantity). And then the 3T3-L1 adipocytes were treated for OA at concentration of 100 micromol/L for 24h with/without the affect of PPARgamma inhibitor GW9662, to determine the gene and protein expression of adiponectin. The protein expression changes of adiponectin were detected by immunoblotting.
  
  
  RESULTS
  The mRNA expression of adiponectin and PPARgamma increased at the dosages of 25, 50, and 100micromol /L. With the increase of OA, the mRNA expression of adiponectin and PPARgamma decreased in a dose-dependent manner. Incubating with GW9662 and OA, decreases in the mRNA and protein expression of adiponectin were 77% and 78.01% (P < 0.05), compared with the control respectively.
  
  
  CONCLUSIONS
  OA could increase the gene expression of adiponectin and PPARgamma mRNA in dose-dependent manner in 3T3-L1 adipocytes,which could be specifically blocked by GW9662, OA indues the expression of adiponectin mRNA and protein in 3T3-L1 adipocytes, possibly by a mechanism involving PPARgamma.
authors: Department of Nutrition and Food Hygiene, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China. weiluolan@163.com
authors: Wei
authors: W
authors: Luo
authors: Jieyi
authors: J
authors: Lin
authors: Yi
authors: Y
authors: Zhang
authors: Jingyi
authors: J
authors: Ouyang
authors: Limei
authors: L
authors: Mao
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism; D052242:Adiponectin / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D051379:Mice; D019301:Oleic Acid / Q000494:pharmacology*; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D052242:Adiponectin; D047495:PPAR gamma; D012333:RNA, Messenger; D019301:Oleic Acid