title: SATB1 is overexpressed in metastatic prostate cancer and promotes prostate cancer cell growth and invasion.
abstract: BACKGROUND
  Special AT-rich sequence binding protein 1 (SATB1) is a nuclear factor that functions as the global chromatin organizer to regulate chromatin structure and gene expression gene expression. SATB1 has been shown to be abnormally expressed in various types of cancer. However, the expression and role of SATB1 in prostate cancer remain unclear.
  
  
  METHODS
  120 cases of prostatic carcinoma and 60 cases of benign prostate hyperplasia were analyzed for SATB1 expression by immunohistochemistry. LNCaP, DU-145, and PC3 prostate cancer cells were examined for SATB1 expression by Western blot analysis. Cell proliferation and invasion was evaluated by CCK8 and transwell invasion assay, respectively.
  
  
  RESULTS
  SATB1 staining was stronger in prostatic carcinomas with metastasis than in those without metastasis, but was absent in benign prostate hyperplasia. Furthermore, SATB1 expression was positively correlated with bone metastasis and the Gleason score. SATB1 overexpression promoted the proliferation and invasion of LNCaP cells while SATB1 knockdown inhibited the proliferation and invasion of DU-145 cells.
  
  
  CONCLUSIONS
  These findings provide novel insight into oncogenic role of SATB1 in prostate cancer, suggesting that SATB1 is a promising biomarker and therapeutic target for prostate cancer.
authors: Department of Urology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China. maolijunxz@163.com
authors: Lijun
authors: L
authors: Mao
authors: Chunhua
authors: C
authors: Yang
authors: Junqi
authors: J
authors: Wang
authors: Wang
authors: W
authors: Li
authors: Rumin
authors: R
authors: Wen
authors: Jiacun
authors: J
authors: Chen
authors: Junnian
authors: J
authors: Zheng
mesh_terms: D014408:Biomarkers, Tumor; D001859:Bone Neoplasms / Q000556:secondary; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002843:Chromatin / Q000378:metabolism*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D036961:Matrix Attachment Region Binding Proteins / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D020411:Oligonucleotide Array Sequence Analysis; D011470:Prostatic Hyperplasia / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D002843:Chromatin; D036961:Matrix Attachment Region Binding Proteins; C076189:SATB1 protein, human
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