title: Demonstration of cytotoxicity against wasps by pierisin-1: a possible defense factor in the cabbage white butterfly.
abstract: The cabbage white butterfly, Pieris rapae, produces pierisin-1, a protein inducing apoptosis of mammalian cells. In the present study, the biological activity of pierisin-1 as a protective agent against parasitic wasps for P. rapae was examined. Pierisin-1 caused detrimental effects on eggs and larvae of non-habitual parasitoids for P. rapae, Glyptapanteles pallipes, Cotesia kariyai and Cotesia plutellae at 1-100 µg/ml, levels essentially equivalent to those found in P. rapae larvae. In contrast, eggs and larvae of the natural parasitoid of P. rapae, Cotesia glomerata proved resistant to the toxicity of pierisin-1 through inhibition of pierisin-1 penetration of the surface layer. The expression level of pierisin-1 mRNA in the larvae of P. rapae was increased by parasitization by C. plutellae, whereas it was decreased by C. glomerata. In addition, C. plutellae was associated with elevation of activated pierisin-1 in the hemolymph. From these observations, it is suggested that pierisin-1 could contribute as a defense factor against parasitization by some type of wasps in P. rapae.
authors: Cancer Prevention Basic Research Project, National Cancer Center Research Institute, Tokyo, Japan. azusan_takahashi@faculty.chiba-u.jp
authors: Azusa
authors: A
authors: Takahashi-Nakaguchi
authors: Yasuko
authors: Y
authors: Matsumoto
authors: Masafumi
authors: M
authors: Yamamoto
authors: Kikuo
authors: K
authors: Iwabuchi
authors: Yukari
authors: Y
authors: Totsuka
authors: Takashi
authors: T
authors: Sugimura
authors: Keiji
authors: K
authors: Wakabayashi
mesh_terms: D036002:ADP Ribose Transferases / Q000235:genetics / Q000633:toxicity*; D000818:Animals; D003603:Cytotoxins / Q000235:genetics / Q000633:toxicity*; D005260:Female; D005786:Gene Expression Regulation; D006458:Hemolymph / Q000378:metabolism; D019476:Insect Proteins / Q000235:genetics / Q000633:toxicity*; D007814:Larva / Q000166:cytology / Q000187:drug effects / Q000469:parasitology; D007915:Lepidoptera / Q000235:genetics / Q000378:metabolism* / Q000469:parasitology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014863:Wasps / Q000166:cytology / Q000187:drug effects* / Q000502:physiology*
chemical_list: D003603:Cytotoxins; D019476:Insect Proteins; D012333:RNA, Messenger; C113557:pierisin protein, insect; D036002:ADP Ribose Transferases
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