title: Environmental particulate matter induces murine intestinal inflammatory responses and alters the gut microbiome.
abstract: BACKGROUND
  Particulate matter (PM) is a key pollutant in ambient air that has been associated with negative health conditions in urban environments. The aim of this study was to examine the effects of orally administered PM on the gut microbiome and immune function under normal and inflammatory conditions.
  
  
  METHODS
  Wild-type 129/SvEv mice were gavaged with Ottawa urban PM10 (EHC-93) for 7-14 days and mucosal gene expression analyzed using Ingenuity Pathways software. Intestinal permeability was measured by lactulose/mannitol excretion in urine. At sacrifice, segments of small and large intestine were cultured and cytokine secretion measured. Splenocytes were isolated and incubated with PM10 for measurement of proliferation. Long-term effects of exposure (35 days) on intestinal cytokine expression were measured in wild-type and IL-10 deficient (IL-10(-/-)) mice. Microbial composition of stool samples was assessed using terminal restriction fragment length polymorphism. Short chain fatty acids were measured in caecum.
  
  
  RESULTS
  Short-term treatment of wild-type mice with PM10 altered immune gene expression, enhanced pro-inflammatory cytokine secretion in the small intestine, increased gut permeability, and induced hyporesponsiveness in splenocytes. Long-term treatment of wild-type and IL-10(-/-) mice increased pro-inflammatory cytokine expression in the colon and altered short chain fatty acid concentrations and microbial composition. IL-10(-/-) mice had increased disease as evidenced by enhanced histological damage.
  
  
  CONCLUSIONS
  Ingestion of airborne particulate matter alters the gut microbiome and induces acute and chronic inflammatory responses in the intestine.
authors: Department of Medicine, University of Alberta, Edmonton, Alberta, Canada.
authors: Lisa
authors: L
authors: Kish
authors: Naomi
authors: N
authors: Hotte
authors: Gilaad G
authors: GG
authors: Kaplan
authors: Renaud
authors: R
authors: Vincent
authors: Robert
authors: R
authors: Tso
authors: Michael
authors: M
authors: Gänzle
authors: Kevin P
authors: KP
authors: Rioux
authors: Aducio
authors: A
authors: Thiesen
authors: Herman W
authors: HW
authors: Barkema
authors: Eytan
authors: E
authors: Wine
authors: Karen L
authors: KL
authors: Madsen
mesh_terms: D000393:Air Pollutants / Q000009:adverse effects*; D000818:Animals; D003106:Colon / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D016207:Cytokines / Q000378:metabolism; D004781:Environmental Exposure / Q000009:adverse effects; D005232:Fatty Acids, Volatile / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D007249:Inflammation / Q000209:etiology; D007413:Intestinal Mucosa / Q000378:metabolism; D007421:Intestine, Small / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D007422:Intestines / Q000276:immunology* / Q000382:microbiology* / Q000473:pathology; D051379:Mice; D018345:Mice, Knockout; D064307:Microbiota; D052638:Particulate Matter / Q000008:administration & dosage / Q000009:adverse effects*; D010539:Permeability; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013997:Time Factors
chemical_list: D000393:Air Pollutants; D016207:Cytokines; D005232:Fatty Acids, Volatile; D052638:Particulate Matter
references: Br J Ind Med. 1992 May;49(5):337-44
references: 1376139
references: Proc Nutr Soc. 2002 Feb;61(1):123-30
references: 12002786
references: Inflamm Bowel Dis. 2007 Jun;13(6):675-83
references: 17262808
references: Free Radic Biol Med. 2003 Jun 1;34(11):1369-82
references: 12757847
references: Toxicol Appl Pharmacol. 1997 Oct;146(2):180-8
references: 9344885
references: Am J Physiol. 1998 Mar;274(3):L396-403
references: 9530175
references: Lancet. 2002 Oct 19;360(9341):1233-42
references: 12401268
references: Am J Respir Crit Care Med. 1994 Jun;149(6):1518-25
references: 8004308
references: BMC Gastroenterol. 2012 May 24;12:51
references: 22624994
references: PLoS One. 2012;7(5):e37932
references: 22649567
references: Adv Drug Deliv Rev. 2000 Jun 30;41(3):251-3
references: 10854684
references: Cell Host Microbe. 2008 Oct 16;4(4):337-49
references: 18854238
references: Genome Biol. 2002 Jun 18;3(7):RESEARCH0034
references: 12184808
references: Gastroenterology. 1998 Jan;114(1):83-92
references: 9428222
references: Mod Pathol. 1989 May;2(3):222-6
references: 2548180
references: PLoS One. 2012;7(10):e47669
references: 23118887
references: J Allergy Clin Immunol. 2012 Apr;129(4):1116-25.e6
references: 22227418
references: Curr Issues Intest Microbiol. 2001 Mar;2(1):17-25
references: 11709853
references: Am J Respir Cell Mol Biol. 1998 Sep;19(3):366-78
references: 9730864
references: Toxicol Appl Pharmacol. 1999 May 15;157(1):43-50
references: 10329506
references: Gastroenterology. 1999 May;116(5):1107-14
references: 10220502
references: Res Rep Health Eff Inst. 2000 Jun;94(Pt 2):5-70; discussion 71-9
references: 11354823
references: Am J Respir Cell Mol Biol. 2001 Sep;25(3):265-71
references: 11588002
references: Am J Physiol Lung Cell Mol Physiol. 2000 Nov;279(5):L924-31
references: 11053029
references: Gut. 2009 Jan;58(1):41-8
references: 18829978
references: Curr Atheroscler Rep. 2010 Sep;12(5):291-300
references: 20617466
references: PLoS One. 2011;6(11):e27225
references: 22073293
references: Inflamm Bowel Dis. 2011 May;17(5):1138-45
references: 20806342
references: Environ Monit Assess. 2013 Feb;185(2):1199-213
references: 22527461
references: Am J Physiol Gastrointest Liver Physiol. 2008 Nov;295(5):G1025-34
references: 18787064
references: Am J Med Sci. 2007 Apr;333(4):230-4
references: 17435417
references: Gut. 2006 Oct;55(10):1512-20
references: 16966705
references: Gastroenterology. 2001 Sep;121(3):580-91
references: 11522742
references: Am J Gastroenterol. 2010 Nov;105(11):2412-9
references: 20588264
references: Gastroenterology. 2002 Jan;122(1):44-54
references: 11781279
references: Appl Environ Microbiol. 1997 Nov;63(11):4516-22
references: 9361437
references: Food Addit Contam. 2005 Jul;22(7):624-41
references: 16019838
references: Gut. 2006 Feb;55(2):205-11
references: 16188921
references: Environ Health Perspect. 2009 Nov;117(11):1780-5
references: 20049132
references: Inflamm Bowel Dis. 2002 Nov;8(6):390-8
references: 12454614
references: Aliment Pharmacol Ther. 2008 Jan 15;27(2):104-19
references: 17973645
references: Cell. 1993 Oct 22;75(2):263-74
references: 8402911
references: Gastroenterology. 2000 Jun;118(6):1094-105
references: 10833484
references: Gut. 2003 Oct;52(10):1442-7
references: 12970137
references: Immunity. 2008 Oct 17;29(4):637-49
references: 18835196
references: CMAJ. 2009 Oct 27;181(9):591-7
references: 19805497
references: Part Fibre Toxicol. 2005 Aug 17;2:4
references: 16105184
references: Toxicol Appl Pharmacol. 1996 Dec;141(2):637-48
references: 8975789
references: Sci Total Environ. 2012 Mar 1;419:25-36
references: 22285091
references: Circulation. 2010 Jun 1;121(21):2331-78
references: 20458016
references: Environ Health Perspect. 2007 May;115(5):728-33
references: 17520060
references: Proc Natl Acad Sci U S A. 2007 Aug 21;104(34):13780-5
references: 17699621
references: Free Radic Res. 2008 Aug;42(8):707-15
references: 18661426
references: Br J Nutr. 2004 Dec;92(6):947-55
references: 15613257
references: Nucleic Acids Res. 2009 Jan;37(Database issue):D141-5
references: 19004872
references: J Crohns Colitis. 2011 Aug;5(4):279-86
references: 21683297
references: Inhal Toxicol. 2005 Jan;17(1):53-65
references: 15764483
references: PLoS One. 2012;7(10):e47416
references: 23082164
references: Fundam Appl Toxicol. 1997 Sep;39(1):18-32
references: 9325024
references: Part Fibre Toxicol. 2011 Jun 09;8:19
references: 21658250
references: J Clin Microbiol. 2005 Jul;43(7):3380-9
references: 16000463
references: Infect Immun. 2012 Aug;80(8):2632-44
references: 22615241
references: J Appl Physiol (1985). 2004 Dec;97(6):2200-6
references: 15347631
references: J Toxicol Environ Health A. 2007 May 15;70(10):820-8
references: 17454558
references: J Immunol. 2012 Mar 15;188(6):2778-93
references: 22345648
references: Science. 2005 Jun 10;308(5728):1635-8
references: 15831718
references: Nat Clin Pract Cardiovasc Med. 2009 Jan;6(1):36-44
references: 19029991
references: Microb Ecol. 2007 May;53(4):562-70
references: 17406775
references: Am J Pathol. 1997 Dec;151(6):1563-70
references: 9403707
references: Part Fibre Toxicol. 2005 Oct 21;2:10
references: 16242040
references: FEMS Microbiol Ecol. 2008 Jul;65(1):169-78
references: 18503550