title: Malonyl-ginsenoside content of a cell-suspension culture of Panax japonicus var. repens.
abstract: The presence of large amounts of ginsenosides malonyl-Rb1, -Rc, -Rb2, and -Rd in a suspension culture of Panax japonicus var. repens cells was demonstrated for the first time. Identification of ginsenoside malonyl-Rb1 was based on chromatographic, chemical, and spectroscopic evidence. Ginsenosides malonyl-Rc, -Rb2, and -Rd were identified on the basis of chromatographic and chemical data. Content and composition of the individual ginsenosides (Rg1, R0, malonyl-Rb1, Rb1, Rc, Rb2, and Rd) were monitored in the suspension culture over 4 years. The RP-HPLC-UV analysis showed that Rg1, R0, and malonyl-Rb1 accounted for more than 75% of the total pool of ginsenosides. In accordance with this result, and data analysis reported in the literature, we propose that ginsenoside formation in the cells of P. japonicus var. repens in vitro is closely related to the cellular compartmentation of these substances. In particular, the accumulation of the 20(S)-protopanaxadiol ginsenosides (especially Rb1) is strongly dependent on their pattern of malonylation, which likely targets them for transport into the vacuole.
authors: Faculty of Biology, Lomonosov Moscow State University, Leninskie Gory 1-12, 119234 Moscow, Russia. dmitry-kochkin@mail.ru
authors: Dmitry V
authors: DV
authors: Kochkin
authors: Vadim V
authors: VV
authors: Kachala
authors: Alexander S
authors: AS
authors: Shashkov
authors: Alexander O
authors: AO
authors: Chizhov
authors: Vasily Y
authors: VY
authors: Chirva
authors: Alexander M
authors: AM
authors: Nosov
mesh_terms: D018929:Cell Culture Techniques; D002478:Cells, Cultured; D036145:Ginsenosides / Q000032:analysis* / Q000737:chemistry; D008968:Molecular Conformation; D005894:Panax / Q000166:cytology*; D059828:Plant Cells / Q000737:chemistry*; D013535:Suspensions / Q000737:chemistry
chemical_list: D036145:Ginsenosides; D013535:Suspensions
keywords: Araliaceae; Cell culture; Compartmentation; Malonyl-ginsenosides; Panax japonicus; Triterpene glycosides