title: Nanofiber-based polyethersulfone scaffold and efficient differentiation of human induced pluripotent stem cells into osteoblastic lineage.
abstract: Human induced pluripotent stem cells (iPSCs) have been shown to have promising potential for regenerative medicine and tissue engineering applications. In the present study, osteogenic differentiation of human iPSCs was evaluated on polyethersulfone (PES) nanofibrous scaffold. According to the results, higher significant expressions of common osteogenic-related genes such as runx2, collagen type I, osteocalcin and osteonectin was observed in PES seeded human iPSCs compared with control. Alizarin red staining and alkaline phosphatase activity of differentiated iPSCs demonstrated significant osteoblastic differentiation potential of these cells. In this study biocompatibility of PES nanofibrous scaffold confirmed by flattened and spreading morphology of iPSCs under osteoblastic differentiation inductive culture. Taking together, nanofiber-based PES scaffold seeded iPSCs showed the highest capacity for differentiation into osteoblasts-like cells. These cells and PES scaffold were demonstrated to have great efficiency for treatment of bone damages and lesions.
authors: Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran. r.ardeshiry.62@gmail.com
authors: Abdolreza
authors: A
authors: Ardeshirylajimi
authors: Saman
authors: S
authors: Hosseinkhani
authors: Kazem
authors: K
authors: Parivar
authors: Parichehr
authors: P
authors: Yaghmaie
authors: Masoud
authors: M
authors: Soleimani
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D058732:Embryoid Bodies; D004789:Enzyme Activation; D015870:Gene Expression; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D057139:Nanofibers / Q000648:ultrastructure; D010006:Osteoblasts / Q000166:cytology* / Q000378:metabolism; D011108:Polymers; D013450:Sulfones; D054457:Tissue Scaffolds
chemical_list: D011108:Polymers; D013450:Sulfones; C022840:polyether sulfone; D000469:Alkaline Phosphatase
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