title: Infectious virion capture by HIV-1 gp120-specific IgG from RV144 vaccinees.
abstract: The detailed examination of the antibody repertoire from RV144 provides a unique template for understanding potentially protective antibody functions. Some potential immune correlates of protection were untested in the correlates analyses due to inherent assay limitations, as well as the need to keep the correlates analysis focused on a limited number of endpoints to achieve statistical power. In an RV144 pilot study, we determined that RV144 vaccination elicited antibodies that could bind infectious virions (including the vaccine strains HIV-1 CM244 and HIV-1 MN and an HIV-1 strain expressing transmitted/founder Env, B.WITO.c). Among vaccinees with the highest IgG binding antibody profile, the majority (78%) captured the infectious vaccine strain virus (CM244), while a smaller proportion of vaccinees (26%) captured HIV-1 transmitted/founder Env virus. We demonstrated that vaccine-elicited HIV-1 gp120 antibodies of multiple specificities (V3, V2, conformational C1, and gp120 conformational) mediated capture of infectious virions. Although capture of infectious HIV-1 correlated with other humoral immune responses, the extent of variation between these humoral responses and virion capture indicates that virion capture antibodies occupy unique immunological space.
authors: Duke Human Vaccine Institute, Duke University, Durham, North Carolina, USA.
authors: Pinghuang
authors: P
authors: Liu
authors: Nicole L
authors: NL
authors: Yates
authors: Xiaoying
authors: X
authors: Shen
authors: Mattia
authors: M
authors: Bonsignori
authors: M Anthony
authors: MA
authors: Moody
authors: Hua-Xin
authors: HX
authors: Liao
authors: Youyi
authors: Y
authors: Fong
authors: S Munir
authors: SM
authors: Alam
authors: R Glenn
authors: RG
authors: Overman
authors: Thomas
authors: T
authors: Denny
authors: Guido
authors: G
authors: Ferrari
authors: Christina
authors: C
authors: Ochsenbauer
authors: John C
authors: JC
authors: Kappes
authors: Victoria R
authors: VR
authors: Polonis
authors: Punnee
authors: P
authors: Pitisuttithum
authors: Jaranit
authors: J
authors: Kaewkungwal
authors: Sorachai
authors: S
authors: Nitayaphan
authors: Supachai
authors: S
authors: Rerks-Ngarm
authors: David C
authors: DC
authors: Montefiori
authors: Peter
authors: P
authors: Gilbert
authors: Nelson L
authors: NL
authors: Michael
authors: Jerome H
authors: JH
authors: Kim
authors: Barton F
authors: BF
authors: Haynes
authors: Georgia D
authors: GD
authors: Tomaras
mesh_terms: D016915:AIDS Vaccines / Q000008:administration & dosage; D057134:Antibodies, Neutralizing / Q000276:immunology; D000914:Antibodies, Viral / Q000276:immunology*; D000918:Antibody Specificity; D015699:HIV Envelope Protein gp120 / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology*; D010865:Pilot Projects; D014765:Viral Vaccines / Q000276:immunology*; D014771:Virion / Q000276:immunology*
chemical_list: D016915:AIDS Vaccines; C112734:AIDSVAX; D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D015699:HIV Envelope Protein gp120; D007074:Immunoglobulin G; D014765:Viral Vaccines
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