title: Metformin inhibits glioma cell U251 invasion by downregulation of fibulin-3.
abstract: Fibulin-3 has been considered as a regulator of glioma cell invasion, but little is known about the molecules regulating fibulin-3 expression. Metformin, an oral antidiabetic drug in the biguanide class, is known to inhibit proliferation and metastasis in a variety of cancer cells. In the present study, we determined the effect of metformin on the expression of fibulin-3 in U251 Human glioma cells. Metformin potently suppressed U251 cell adhesion and invasion. Metformin inhibited the expression of fibulin-3 at the transcriptional level. Moreover, metformin abolished the protein expression of fibulin-3 in a concentration-dependent manner. Furthermore, this compound suppressed the expression of matrix metalloproteinase-2, a key effector of glioma cell invasion, regulated by fibulin-3. Taken together, our results suggest that metformin abolishes fibulin-3 expression and subsequently inhibits invasion of glioma cells.
authors: Department of Neurology, The 4th Affiliated Hospital of China Medical University, Shenyang, China. dr.gao_cmu@yahoo.cn
authors: Lian-Bo
authors: LB
authors: Gao
authors: Shen
authors: S
authors: Tian
authors: Hong-Hua
authors: HH
authors: Gao
authors: Yan-Yuan
authors: YY
authors: Xu
mesh_terms: D000199:Actins / Q000096:biosynthesis / Q000235:genetics; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000473:pathology*; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis* / Q000235:genetics; D005910:Glioma / Q000473:pathology*; D006801:Humans; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis / Q000235:genetics; D008687:Metformin / Q000494:pharmacology*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D000199:Actins; C119630:EFEMP1 protein, human; D016326:Extracellular Matrix Proteins; D007004:Hypoglycemic Agents; D008687:Metformin; D020778:Matrix Metalloproteinase 2