title: Method Development and Validation of a Stability-Indicating RP-HPLC Method for the Quantitative Analysis of Dronedarone Hydrochloride in Pharmaceutical Tablets.
abstract: A simple, precise, and accurate HPLC method has been developed and validated for the quantitative analysis of Dronedarone Hydrochloride in tablet form. An isocratic separation was achieved using a Waters Symmetry C8 (100 × 4.6 mm), 5 μm particle size column with a flow rate of 1 ml/min and UV detector at 290 nm. The mobile phase consisted of buffer: methanol (40:60 v/v) (buffer: 50 mM KH2PO4 + 1 ml triethylamine in 1 liter water, pH=2.5 adjusted with ortho-phosphoric acid). The method was validated for specificity, linearity, precision, accuracy, robustness, and solution stability. The specificity of the method was determined by assessing interference from the placebo and by stress testing the drug (forced degradation). The method was linear over the concentration range 20-80 μg/ml (r(2) = 0.999) with a Limit of Detection (LOD) and Limit of Quantitation (LOQ) of 0.1 and 0.3 μg/ml respectively. The accuracy of the method was between 99.2-100.5%. The method was found to be robust and suitable for the quantitative analysis of Dronedarone Hydrochloride in a tablet formulation. Degradation products resulting from the stress studies did not interfere with the detection of Dronedarone Hydrochloride so the assay is thus stability-indicating.
authors: Department of Chemistry, Saurashtra University, Rajkot-360 005, Gujarat, India.
authors: Batuk
authors: B
authors: Dabhi
authors: Yashwantsinh
authors: Y
authors: Jadeja
authors: Madhavi
authors: M
authors: Patel
authors: Hetal
authors: H
authors: Jebaliya
authors: Denish
authors: D
authors: Karia
authors: Anamik
authors: A
authors: Shah
keywords: Dronedarone Hydrochloride; HPLC; Stability-indicating
references: J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Jun 15;877(18-19):1727-31
references: 19427276
references: J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Oct 15;879(28):3071-5
references: 21937291