title: Clostridium botulinum strain Af84 contains three neurotoxin gene clusters: bont/A2, bont/F4 and bont/F5.
abstract: Sanger and shotgun sequencing of Clostridium botulinum strain Af84 type Af and its botulinum neurotoxin gene (bont) clusters identified the presence of three bont gene clusters rather than the expected two. The three toxin gene clusters consisted of bont subtypes A2, F4 and F5. The bont/A2 and bont/F4 gene clusters were located within the chromosome (the latter in a novel location), while the bont/F5 toxin gene cluster was located within a large 246 kb plasmid. These findings are the first identification of a C. botulinum strain that contains three botulinum neurotoxin gene clusters.
authors: Infant Botulism Treatment and Prevention Program, California Department of Public Health, Richmond, California, United States of America.
authors: Nir
authors: N
authors: Dover
authors: Jason R
authors: JR
authors: Barash
authors: Karen K
authors: KK
authors: Hill
authors: Karen W
authors: KW
authors: Davenport
authors: Hazuki
authors: H
authors: Teshima
authors: Gary
authors: G
authors: Xie
authors: Stephen S
authors: SS
authors: Arnon
mesh_terms: D000818:Animals; D001905:Botulinum Toxins / Q000235:genetics*; D019274:Botulinum Toxins, Type A / Q000235:genetics*; D002876:Chromosomes, Bacterial / Q000235:genetics; D003014:Clostridium botulinum / Q000235:genetics*; D023281:Genomics; D051379:Mice; D005810:Multigene Family / Q000235:genetics*; D009498:Neurotoxins / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D017421:Sequence Analysis
chemical_list: D009498:Neurotoxins; D001905:Botulinum Toxins; D019274:Botulinum Toxins, Type A; C096321:botulinum toxin type F
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