title: The role of IL15 gene variants in visceral leishmaniasis among Iranian patients.
abstract: The role of IL-15 in the protection against Leishmania (L) parasites has been clarified in previous studies, in which IL-15 similar to IFN-γ induces IL-12 production and stimulates the leishmaniacidal activity of the macrophages infected with L. infantum. Furthermore, the increased level of IL-15 in acute visceral leishmaniasis patients (VL) can suppress Th2 cytokines such as IL-4. Since different single nucleotide polymorphisms (SNPs) in the IL15 gene have been described, this study aimed to investigate the association of the SNPs at the positions 267, 367, 13,687 and 14,035 with VL. The IL15 gene variants were compared between two groups consisting of 117 VL patients and 146 healthy individuals using polymerase chain reaction-restriction fragment length polymorphism. The results showed that the frequencies of the alleles 267C (83.9 vs. 73.5%, P=0.0035), 13687A (22.4 vs. 12.8%, P=0.032), genotype 267CC (68.5 vs. 55.6%, P=0.031), haplotypes CGCA (16 vs. 8.3%, P=0.02) and TACA (11.2 vs. 4.8%, P=0.02) were significantly higher in the controls than those in the patients, while the genotypes 267TT (8.5 vs. 0.7%, P=0.0016), 13687CC (78.6 vs. 65.5%, P=0.015), the haplotypes TGCT (10 vs. 2.5%, P=0.00002) and TGCA (5.7 vs. 0.35%, P=0.000001) were significantly more frequent in the patients. In conclusion, it may be speculated that these gene variants with probable effects on the IL-15 production can serve as the factors influencing VL among Iranian population. However, to clarify the association of these variants with the level of IL-15, further studies are recommended.
authors: Department of Immunology, Professor Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. rasouliman@yahoo.com
authors: Manoochehr
authors: M
authors: Rasouli
authors: Mehdi
authors: M
authors: Kalani
authors: Simin
authors: S
authors: Kiany
mesh_terms: D000293:Adolescent; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D017931:DNA Primers / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D007223:Infant; D019409:Interleukin-15 / Q000235:genetics* / Q000378:metabolism; D007492:Iran; D007898:Leishmaniasis, Visceral / Q000235:genetics* / Q000378:metabolism; D008297:Male; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: D017931:DNA Primers; D019409:Interleukin-15
references: Infect Genet Evol. 2011 Jul;11(5):912-6
references: 21376140
references: J Exp Med. 1994 Oct 1;180(4):1395-403
references: 7523571
references: Am J Trop Med Hyg. 2006 Feb;74(2):306-7
references: 16474088
references: J Clin Invest. 1985 Dec;76(6):2066-9
references: 3935667
references: Mol Biol Rep. 2012 Jun;39(6):6907-14
references: 22311026
references: Epidemiol Infect. 1995 Apr;114(2):297-318
references: 7705493
references: Genes Immun. 2007 Jan;8(1):84-90
references: 17122780
references: Am J Trop Med Hyg. 1999 Dec;61(6):941-4
references: 10674674
references: J Med Genet. 1999 Apr;36(4):295-9
references: 10227396
references: Annu Rev Immunol. 1999;17:19-49
references: 10358752
references: J Clin Invest. 1995 Dec;96(6):2578-82
references: 8675621
references: Cytokine. 2007 May;38(2):84-9
references: 17616399
references: J Allergy Clin Immunol. 2004 May;113(5):896-901
references: 15131572
references: J Immunol. 1997 Jan 15;158(2):800-6
references: 8992997
references: Mol Biol Rep. 2012 Dec;39(12):10795-802
references: 23053976
references: Science. 1995 Nov 17;270(5239):1215-8
references: 7502050
references: J Immunol. 1995 Jan 15;154(2):483-90
references: 7814861
references: Cytokine. 2010 Jan;49(1):58-63
references: 19796965
references: J Immunol. 1987 Feb 1;138(3):908-13
references: 3100620
references: J Invest Dermatol. 2007 Nov;127(11):2544-51
references: 17554368
references: Am J Trop Med Hyg. 1995 Apr;52(4):364-5
references: 7741179
references: Trans R Soc Trop Med Hyg. 1982;76(6):741-6
references: 6984547
references: Blood Cells Mol Dis. 2004 Jul-Aug;33(1):45-50
references: 15223010
references: Clin Exp Immunol. 2002 Feb;127(2):360-5
references: 11876762
references: Am J Trop Med Hyg. 1994 Dec;51(6):826-36
references: 7810819
references: Scand J Immunol. 2004 Dec;60(6):609-14
references: 15584972
references: Infect Immun. 2002 Dec;70(12):6919-25
references: 12438370
references: Genet Epidemiol. 2001 Apr;20(3):383-96
references: 11255246
references: J Leukoc Biol. 1995 May;57(5):763-6
references: 7759955
references: Annu Rev Immunol. 1995;13:151-77
references: 7612219
references: Biometrics. 1997 Dec;53(4):1253-61
references: 9423247
references: Am J Trop Med Hyg. 2004 Nov;71(5):561-7
references: 15569785
references: Cytokine. 2001 Jan 21;13(2):119-23
references: 11145853
references: Microbes Infect. 2000 Apr;2(4):381-9
references: 10817640
references: Immunol Today. 1996 Jan;17(1):13-6
references: 8652043
references: Pak J Biol Sci. 2007 Jun 1;10(11):1850-4
references: 19086549
references: J Immunol. 1991 Feb 1;146(3):1026-30
references: 1824848
references: Tissue Antigens. 2011 Nov;78(5):352-8
references: 21988722