title: Suppression of TLR-4-related inflammatory pathway and anti-fibrosis effects of probiotic-fermented purple sweet potato yogurt in hearts of spontaneously hypertensive rats.
abstract: Inflammation plays an important role in triggering fibrosis of cardiovascular disease and hypertension. Gamma-aminobutyric acid (GABA) has hypotensive effect; GABA concentration could be enhanced in milk fermented with lactic acid bacteria (LAB). This study evaluated the effect of probiotic-fermented purple sweet potato yogurt (PSPY) on the toll-like receptor 4 (TLR-4)-related inflammatory components, and on fibrosis in the heart of spontaneously hypertensive rat (SHR). TLR4-related pathway and fibrosis-associated proteins TGFbeta and FGF2 were significantly increased in SHR hearts, but were highly suppressed in 10% PSPY-fed rats. Microscopic examination with Masson trichrome staining of left ventricle further demonstrated that 10% and 100% PSPY both significantly reduced interstitial fibrosis in SHR hearts. These findings indicated that oral administration of 10% probiotic-fermented PSPY was strong enough to lower cardiac fibrosis in SHR rats through the suppression of TLR-4-related inflammatory pathway. Therefore, PSPY may be included in diets to help prevent cardiac fibrosis in patients with hypertension.
authors: Department of Food and Nutrition, Providence University, Taichung 43301, Taiwan, Republic of China.
authors: Pei-Pei
authors: PP
authors: Lin
authors: Department of Food and Nutrition, Providence University, Taichung 43301, Taiwan, Republic of China.
authors: You-Miin
authors: YM
authors: Hsieh
authors: Department of Biological Science and Technology, China Medical University, Taichung 40402, Taiwan, Republic of China.
authors: Wei-Wen
authors: WW
authors: Kuo
authors: Department of Pathology, Changhua Christian Hospital, Changua 50006, Taiwan, Republic of China.
authors: Yueh-Min
authors: YM
authors: Lin
authors: Department of Pathology, Changhua Christian Hospital, Changua 50006, Taiwan, Republic of China.
authors: Yu-Lan
authors: YL
authors: Yeh
authors: Orthopaedic Department, Armed Force General Hospital, Taipei 10581, Taiwan, Republic of China.
authors: Chien-Chung
authors: CC
authors: Lin
authors: Graduate Institute of Chinese Medical Science, China Medical University, Taichung 40402, Taiwan, Republic of China.
authors: Fuu-Jen
authors: FJ
authors: Tsai
authors: Department of Healthcare Administration, Asia University, Taichung 41354, Taiwan, Republic of China.
authors: Chang-Hai
authors: CH
authors: Tsai
authors: Department of Food Science and Technology, Hung Kuang University, Taichung 43302, Taiwan, Republic of China.
authors: Cheng-Chih
authors: CC
authors: Tsai
authors: Graduate Institute of Chinese Medical Science, China Medical University, Taichung 40402, Taiwan, Republic of China.
authors: Chih-Yang
authors: CY
authors: Huang
mesh_terms: D000818:Animals; D005355:Fibrosis; D006331:Heart Diseases / Q000473:pathology / Q000517:prevention & control*; D006973:Hypertension / Q000178:diet therapy* / Q000473:pathology; D018836:Inflammation Mediators / Q000037:antagonists & inhibitors* / Q000502:physiology; D027723:Ipomoea batatas; D007779:Lactobacillus acidophilus; D048668:Lactobacillus delbrueckii; D008297:Male; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D015398:Signal Transduction; D051197:Toll-Like Receptor 4 / Q000037:antagonists & inhibitors* / Q000502:physiology; D015014:Yogurt
chemical_list: D018836:Inflammation Mediators; C495347:Tlr4 protein, rat; D051197:Toll-Like Receptor 4