title: Bio-microfluidic platform for gold nanoprobe based DNA detection--application to Mycobacterium tuberculosis.
abstract: We have projected and fabricated a microfluidic platform for DNA sensing that makes use of an optical colorimetric detection method based on gold nanoparticles. The platform was fabricated using replica moulding technology in PDMS patterned by high-aspect-ratio SU-8 moulds. Biochips of various geometries were tested and evaluated in order to find out the most efficient architecture, and the rational for design, microfabrication and detection performance is presented. The best biochip configuration has been successfully applied to the DNA detection of Mycobacterium tuberculosis using only 3 µl on DNA solution (i.e. 90 ng of target DNA), therefore a 20-fold reduction of reagents volume is obtained when compared with the actual state of the art.
authors: CENIMAT/I3N, Departamento de Ciência dos Materiais, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Campus de Caparica, 2829-516 Caparica, Portugal.
authors: Iwona
authors: I
authors: Bernacka-Wojcik
authors: Paulo
authors: P
authors: Lopes
authors: Ana
authors: A
authors: Catarina Vaz
authors: Bruno
authors: B
authors: Veigas
authors: Pawel
authors: P
authors: Jerzy Wojcik
authors: Pedro
authors: P
authors: Simões
authors: David
authors: D
authors: Barata
authors: Elvira
authors: E
authors: Fortunato
authors: Pedro
authors: P
authors: Viana Baptista
authors: Hugo
authors: H
authors: Aguas
authors: Rodrigo
authors: R
authors: Martins
mesh_terms: D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D004277:DNA, Single-Stranded / Q000737:chemistry; D004867:Equipment Design; D005336:Fiber Optic Technology / Q000295:instrumentation; D006046:Gold / Q000737:chemistry*; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D053758:Nanoparticles / Q000737:chemistry*; D012680:Sensitivity and Specificity; D014376:Tuberculosis / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial; D004277:DNA, Single-Stranded; D006046:Gold