title: DDA3 associates with microtubule plus ends and orchestrates microtubule dynamics and directional cell migration.
abstract: Cell motility and adhesion involve orchestrated interaction of microtubules (MTs) with their plus-end tracking proteins (+TIPs). However, the mechanisms underlying regulations of MT dynamics and directional cell migration are still elusive. Here, we show that DDA3-EB1 interaction orchestrates MT plus-end dynamics and facilitates directional cell migration. Biochemical characterizations reveal that DDA3 interacts with EB1 via its SxIP motif within the C-terminal Pro/Ser-rich region. Time-lapse and total internal reflection fluorescence (TIRF) microscopic assays demonstrate that DDA3 exhibits EB1-dependent, MT plus-end loading and tracking. The EB1-based loading of DDA3 is responsible for MT plus-ends stabilization at the cell cortex, which in turn orchestrates directional cell migration. Interestingly, the DDA3-EB1 interaction is potentially regulated by EB1 acetylation, which may account for physiological regulation underlying EGF-elicited cell migration. Thus, the EB1-based function of DDA3 links MT dynamics to directional cell migration.
authors: Anhui Key Laboratory of Cellular Dynamics and the University of Science & Technology of China, Hefei, China 230026.
authors: Liangyu
authors: L
authors: Zhang
authors: Hengyi
authors: H
authors: Shao
authors: Tongge
authors: T
authors: Zhu
authors: Peng
authors: P
authors: Xia
authors: Zhikai
authors: Z
authors: Wang
authors: Lifang
authors: L
authors: Liu
authors: Maomao
authors: M
authors: Yan
authors: Donald L
authors: DL
authors: Hill
authors: Guowei
authors: G
authors: Fang
authors: Zhengjun
authors: Z
authors: Chen
authors: Dongmei
authors: D
authors: Wang
authors: Xuebiao
authors: X
authors: Yao
mesh_terms: D001665:Binding Sites; D002465:Cell Movement / Q000502:physiology*; D006367:HeLa Cells; D006801:Humans; D008870:Microtubules / Q000502:physiology*; D020409:Molecular Motor Proteins / Q000502:physiology*; D010750:Phosphoproteins / Q000502:physiology*; D011485:Protein Binding
chemical_list: D020409:Molecular Motor Proteins; C485740:PSRC1 protein, human; D010750:Phosphoproteins
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