title: Anti-glycated and antiradical activities in vitro of polysaccharides from Ganoderma capense.
abstract: BACKGROUND
  Ganoderma capense is a Ganoderma species and is widely used, especially in Asia, as a well-known medicinal mushroom for health-promoting effect and for treatment of chronic diseases, such as diabetes, aging, etc. G. capense is rich of polysaccharide.
  
  
  OBJECTIVE
  To isolate the polysaccharides from G. capense and evaluate their anti-glycated and antiradical activities in vitro.
  
  
  METHODS
  The dried powder of submerged fermentation culturing mycelium of G. capense was defatted, extracted with water/alkaline water followed by ethanol precipitation and deproteinated. And four crude polysaccharides, named as GC50, GC70, GC90 and GCB, were obtained. For the first time, the in vitro anti-glycated activities of the four samples were studied by non-enzymatic glycation reaction. Then, the DPPH radical and hydroxyl radical assays were established to estimate the antiradical capacity of the four samples. Meanwhile the contents of polysaccharides were determined by phenol-sulphuric acid colorimetry.
  
  
  CONCLUSIONS
  Preliminary antiradical in vitro studies indicated that the four crude polysaccharides showed concentration-dependent scavenging abilities on DPPH and hydroxyl radicals. The evaluation of anti-glycation activity suggested that GC70 had good potential for inhibiting the formation of advanced glycation end products. Time- and dose-dependent effects were also observed for all GC70 samples.
authors: Department of Natural Medicinal Chemistry, College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, Peoaple's Republic of China.
authors: Chunyan
authors: C
authors: Yan
authors: Fansheng
authors: F
authors: Kong
authors: Dezhi
authors: D
authors: Zhang
authors: Jiangxia
authors: J
authors: Cui
keywords: Anti-glycated activity; Ganoderma capense (Lloyd) Teng; antiradical activity; in vitro; polysaccharide
references: Phytochemistry. 2006 Sep;67(18):1985-2001
references: 16905165
references: Appl Microbiol Biotechnol. 2006 Sep;72(3):537-43
references: 16411085
references: Nature. 1995 Dec 21-28;378(6559):776-9
references: 8524410
references: Phytother Res. 2005 Apr;19(4):310-3
references: 16041724
references: Trends Cardiovasc Med. 1998 Feb;8(2):59-64
references: 21235913
references: Diabetologia. 2001 Feb;44(2):129-46
references: 11270668
references: J Biol Chem. 1951 Nov;193(1):265-75
references: 14907713
references: Biochem Biophys Res Commun. 2004 Feb 20;314(4):988-93
references: 14751230
references: Free Radic Biol Med. 1991;10(5):339-52
references: 1855674