title: [Comparison of two nucleic acid extraction methods for norovirus in oysters].
abstract: OBJECTIVE
  To explore a convenient and effective method for norovirus nucleic acid extraction from oysters suitable for long-term viral surveillance.
  
  
  METHODS
  Two methods, namely method A (glycine washing and polyethylene glycol precipitation of the virus followed by silica gel centrifugal column) and method B (protease K digestion followed by application of paramagnetic silicon) were compared for their performance in norovirus nucleic acid extraction from oysters. Real-time RT-PCR was used to detect norovirus in naturally infected oysters and in oysters with induced infection.
  
  
  RESULTS
  The two methods yielded comparable positive detection rates for the samples, but the recovery rate of the virus was higher with method B than with method A.
  
  
  CONCLUSIONS
  Method B is a more convenient and rapid method for norovirus nucleic acid extraction from oysters and suitable for long-term surveillance of norovirus.
authors: Sun Yat-Sen University School of Public Health, Guangzhou 510080, China. yuanrachel@sohu.com
authors: Qiao
authors: Q
authors: Yuan
authors: Hui
authors: H
authors: Li
authors: Xiaoling
authors: X
authors: Deng
authors: Yanling
authors: Y
authors: Mo
authors: Ling
authors: L
authors: Fang
authors: Changwen
authors: C
authors: Ke
mesh_terms: D000818:Animals; D002498:Centrifugation / Q000379:methods*; D029322:Norovirus / Q000235:genetics / Q000302:isolation & purification*; D010125:Ostreidae / Q000821:virology*; D012367:RNA, Viral / Q000302:isolation & purification*
chemical_list: D012367:RNA, Viral