title: Direct methods for detection of protein S-nitrosylation.
abstract: S-nitrosylation of protein cysteine residues is known to be an important mechanism for nitric oxide signaling. However, the detection of protein S-nitrosylation is still challenging due to technical limitations of current methods. This chapter provides a brief review on recent developments of methods, which directly target S-nitroso moieties for detection. We also describe in detail the protocol of an organophosphine-based biotin labeling of protein S-nitroso moieties.
authors: Department of Chemistry, Washington State University, Pullman, WA 99164, USA.
authors: Nelmi O
authors: NO
authors: Devarie-Baez
authors: Dehui
authors: D
authors: Zhang
authors: Sheng
authors: S
authors: Li
authors: A Richard
authors: AR
authors: Whorton
authors: Ming
authors: M
authors: Xian
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity; D006801:Humans; D013058:Mass Spectrometry; D009569:Nitric Oxide / Q000502:physiology; D011499:Protein Processing, Post-Translational; D020543:Proteome / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D026403:S-Nitrosothiols / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D013194:Staining and Labeling
chemical_list: D020543:Proteome; D026403:S-Nitrosothiols; D009569:Nitric Oxide
keywords: Bioorthogonal reaction; Nitric oxide; Phosphine; Protein labeling; S-nitrosothiol; S-nitrosylation
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