title: Immunomodulatory and anti-allergic effects of orally administered Lactobacillus species in ovalbumin-sensitized mice.
abstract: We investigated the effects of orally administered probiotic bacteria (Lactobacillus species) as allergic immune modulators in ovalbumin (OVA)-sensitized mice. BALB/c mice were intraperitoneally injected with OVA twice at a 2-week interval for allergy sensitization. The mice were then orally administered Lactobacillus casei YIT9029 (L1), L. casei HY7201 (L2), L. brevis HY7401 (L3), or L. plantarum HY20301 (L4) every 2 days for 3 weeks. Total IgE levels significantly decreased in sera of L3-administered mice but increased in the other groups. OVA-specific IgE levels decreased slightly in sera of mice administered L1, L3, and L4 but increased significantly in L2-administered mice. In passive cutaneous anaphylaxis (PCA) using sera from administered mice, only the L3-administered group showed reaction inhibition. High expression of TLR-2 with interferon (IFN)-gamma stimulation on peripheral blood mononuclear cells occurred in L3- or L4-administered mice. Th1 cytokines, including IFN-gamma and interleukin (IL)- 12, increased in splenocytes of L3-administered mice; however, IL-4 decreased in L1- and L4-administered groups; IL-5 decreased in all experimental groups. IL-6 decreased in the L3-administered group; and IL-10 decreased in L1-, L2-, and L3-administered groups. L3 induced antiallergic effects by increasing Th1 cytokines, decreasing Th2 cytokines, and inhibiting the PCA reaction, whereas L2 administration increased allergic effects.
authors: Laboratory of Immunology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
authors: Jeongmin
authors: J
authors: Lee
authors: Jieun
authors: J
authors: Bang
authors: Hee-Jong
authors: HJ
authors: Woo
mesh_terms: D000284:Administration, Oral; D000818:Animals; D018926:Anti-Allergic Agents / Q000008:administration & dosage*; D016207:Cytokines / Q000276:immunology; D005260:Female; D006801:Humans; D006967:Hypersensitivity / Q000188:drug therapy* / Q000276:immunology*; D007073:Immunoglobulin E / Q000276:immunology; D007155:Immunologic Factors / Q000008:administration & dosage*; D007778:Lactobacillus / Q000145:classification / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000009:adverse effects / Q000276:immunology*; D019936:Probiotics / Q000008:administration & dosage*
chemical_list: D018926:Anti-Allergic Agents; D016207:Cytokines; D007155:Immunologic Factors; D007073:Immunoglobulin E; D010047:Ovalbumin