title: Identification and characterization of a cis-encoded antisense RNA associated with the replication process of Salmonella enterica serovar Typhi.
abstract: Antisense RNAs that originate from the complementary strand of protein coding genes are involved in the regulation of gene expression in all domains of life. In bacteria, some of these antisense RNAs are transcriptional noise while others play a vital role to adapt the cell to changing environmental conditions. By deep sequencing analysis of transcriptome of Salmonella enterica serovar Typhi, a partial RNA sequence encoded in-cis to the dnaA gene was revealed. Northern blot and RACE analysis confirmed the transcription of this antisense RNA which was expressed mostly in the stationary phase of the bacterial growth and also under iron limitation and osmotic stress. Pulse expression analysis showed that overexpression of the antisense RNA resulted in a significant increase in the mRNA levels of dnaA, which will ultimately enhance their translation. Our findings have revealed that antisense RNA of dnaA is indeed transcribed not merely as a by-product of the cell's transcription machinery but plays a vital role as far as stability of dnaA mRNA is concerned.
authors: Department of Biochemistry and Molecular Biology, School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang, Jiangsu, China.
authors: Isaac
authors: I
authors: Dadzie
authors: Shungao
authors: S
authors: Xu
authors: Bin
authors: B
authors: Ni
authors: Xiaolei
authors: X
authors: Zhang
authors: Haifang
authors: H
authors: Zhang
authors: Xiumei
authors: X
authors: Sheng
authors: Huaxi
authors: H
authors: Xu
authors: Xinxiang
authors: X
authors: Huang
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D008969:Molecular Sequence Data; D016372:RNA, Antisense / Q000032:analysis* / Q000235:genetics*; D012329:RNA, Bacterial / Q000032:analysis* / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D038621:Regulatory Sequences, Ribonucleic Acid; D012485:Salmonella typhi / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C066312:DnaA protein, Bacteria; D016372:RNA, Antisense; D012329:RNA, Bacterial; D012333:RNA, Messenger; D038621:Regulatory Sequences, Ribonucleic Acid
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