title: Expression of CCT6A mRNA in chicken granulosa cells is regulated by progesterone.
abstract: CCT6A, the zeta subunit of the chaperonin containing TCP1 complex, is the only cytosolic chaperonin in eukaryotes and is estimated to assist in the folding of multiple proteins including actin, tubulin, cyclin E, myosin, transducin and the Von Hippel Lindau tumor suppressor. In this study, we examined the expression of CCT6A and progesterone receptor (PGR) mRNA in various tissues of chickens and the regulation of CCT6A and PGR mRNA in ovarian granulosa cells. Northern blot analysis revealed that CCT6A had one transcript and was highly expressed in the ovary tissues from chickens at both the sexually immature and mature stages. CCT6A mRNA expression was increased maximally from pre-hierarchy follicles to F5 follicles and subsequently declined in pre-ovulatory and post-ovulatory follicles. The expression of PGR mRNA exhibited the similar pattern to CCT6A. In granulosa cells isolated from pre-ovulatory follicles, follicle-stimulating hormone (FSH) inhibited the expression of CCT6A mRNA, whereas progesterone activated CCT6A and suppressed PGR expression in a time-dependent manner. We further investigated the regulation of CCT6A transcription by progesterone by constructing various progressive deletions and mutants and identified the core promoter element of CCT6A and the binding region of progesterone, which is located from -2056 to -2051. Taken together, our results indicate that CCT6A likely plays an important role in follicle growth, and in granulosa cells, progesterone activates CCT6A transcription via a progesterone response element (PRE) located in the distal promoter of CCT6A.
authors: Laboratory of Animal Molecular Genetics, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China.
authors: Qingqing
authors: Q
authors: Wei
authors: Guiyu
authors: G
authors: Zhu
authors: Xinxing
authors: X
authors: Cui
authors: Li
authors: L
authors: Kang
authors: Dingguo
authors: D
authors: Cao
authors: Yunliang
authors: Y
authors: Jiang
mesh_terms: D000818:Animals; D015152:Blotting, Northern; D002478:Cells, Cultured; D056404:Chaperonin Containing TCP-1 / Q000235:genetics*; D002642:Chick Embryo; D005260:Female; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism*; D011374:Progesterone / Q000494:pharmacology*; D011401:Promoter Regions, Genetic / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D011980:Receptors, Progesterone / Q000235:genetics
chemical_list: D011980:Receptors, Progesterone; D011374:Progesterone; D056404:Chaperonin Containing TCP-1