title: Association between GSTP1, GSTM1 and GSTT1 polymorphisms involved in xenobiotic metabolism and head and neck cancer development.
abstract: Polymorphisms in the glutathione S-transferase superfamily genes that encodes enzymes involved in the phase II xenobiotic metabolism may lead head and neck cancer development. In this study we investigate the association of A313G and C341T GSTP1 polymorphisms, GSTM1 and GSTT1 null genotypes in the head and neck cancer development, interactions between these polymorphisms,the tumor histopathologic parameters and risk factors (smoking and drinking) were also evaluated in the case-control study. 775 individuals (261 patients/514 controls) were included in the study. Molecular analyzes were performed by PCR and PCR-RFLP; and statistical analyzes by Chi square and multiple logistic regression. Chi square test showed that only the genotype frequencies for GSTM1 and GSTT1 were in Hardy-Weinberg disequilibrium in both groups. Significant results with p ≤ 0.05 showed that age ≥ 48 years (OR = 11.87; 7.55-18.65), smoking (OR = 4.25; 2.70-6.69), drinking (OR = 1.59; 1.02-2.46) were possible predictors for the head and neck cancer development and the presence of A313G GSTP1 polymorphism (OR = 0.62; 0.42-0.92) decreased the risk for this disease. Individuals with the 313AG/GG GSTP1 and age ≥ 48 years (OR = 0.59; 0.38-0.91), male gender (OR = 0.54; 0.35-0.83), smokers (OR = 0.63; 0.40-0.99) and drinkers (OR = 0.57; 0.35-0.95); the GSTM1 null genotype and age < 48 years (OR = 2.46; 1.09-5.55); the GSTT1 null genotype and primary anatomical sites of pharynx (OR = 0.37; 0.17-0.79) and larynx (OR = 3.60; 1.93-6.72), can modulate the risk for the disease development. The variables age ≥ 48 years, smoking and drinking can be predictors for head and neck cancer development; moreover, A313G GSTP1 polymorphism, GSTM1 and GSTT1 null genotypes can modulate the risk for this disease.
authors: Department of Molecular Biological and Genetics and Molecular Biology Research Unit-UPGEM, Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto Medical School (FAMERP), Av. Brigadeiro Faria Lima, 5416, São José do Rio Preto, SP, 15090-000, Brazil. aneliserusso@yahoo.com.br
authors: Anelise
authors: A
authors: Russo
authors: Pamela Risardi
authors: PR
authors: Francelin
authors: Ana Lívia Silva
authors: AL
authors: Galbiatti
authors: Luis Sérgio
authors: LS
authors: Raposo
authors: José Victor
authors: JV
authors: Maníglia
authors: Erika Cristina
authors: EC
authors: Pavarino
authors: Eny Maria
authors: EM
authors: Goloni-Bertollo
mesh_terms: D000328:Adult; D000368:Aged; D000483:Alleles; D016022:Case-Control Studies; D005260:Female; D005838:Genotype; D051549:Glutathione S-Transferase pi / Q000235:genetics*; D005982:Glutathione Transferase / Q000235:genetics*; D006258:Head and Neck Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D011110:Polymorphism, Genetic; D012307:Risk Factors; D015262:Xenobiotics / Q000378:metabolism*
chemical_list: D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; D015262:Xenobiotics; C413545:glutathione S-transferase T1
references: Gac Sanit. 2005 Jul-Aug;19(4):321-4
references: 16050969
references: Mutat Res. 2009 Oct 2;669(1-2):36-41
references: 19406142
references: Braz J Otorhinolaryngol. 2006 Sep-Oct;72(5):654-8
references: 17221058
references: Cancer Epidemiol Biomarkers Prev. 2006 Nov;15(11):2196-202
references: 17119046
references: Cancer Prev Res (Phila). 2009 May;2(5):432-9
references: 19401526
references: Nat Rev Cancer. 2011 Jan;11(1):9-22
references: 21160525
references: Head Neck. 2010 Aug;32(8):1079-87
references: 19953622
references: J Nutr. 1998 Apr;128(4):694-700
references: 9521630
references: Mol Biol Rep. 2012 Jan;39(1):635-43
references: 21556759
references: Semin Radiat Oncol. 2010 Jan;20(1):52-7
references: 19959031
references: Int J Cancer. 1999 Mar 31;81(1):44-8
references: 10077151
references: Sao Paulo Med J. 2012;130(5):307-13
references: 23174870
references: Cancer Lett. 1996 Oct 22;107(2):229-33
references: 8947518
references: Cancer Causes Control. 1998 Jan;9(1):99-108
references: 9486469
references: Eur J Cancer. 2007 Dec;43(18):2698-706
references: 17707637
references: Cancer Res. 2000 Jun 15;60(12):3155-9
references: 10866304
references: Cancer Lett. 2002 Dec 10;187(1-2):95-101
references: 12359356
references: Postepy Hig Med Dosw (Online). 2008 Sep 23;62:490-501
references: 18833034
references: Mutat Res. 2008 Feb 1;638(1-2):184-94
references: 18035380
references: Toxicol Sci. 2011 Mar;120 Suppl 1:S49-75
references: 21059794
references: Life Sci. 2011 May 23;88(21-22):959-71
references: 20932495
references: Oral Oncol. 2008 Feb;44(2):200-7
references: 17418613
references: FEBS J. 2011 Nov;278(22):4243-51
references: 21929724
references: Carcinogenesis. 1997 Apr;18(4):641-4
references: 9111193
references: Nucleic Acids Res. 1988 Feb 11;16(3):1215
references: 3344216
references: J Natl Cancer Inst. 2007 May 16;99(10):777-89
references: 17505073
references: Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2010 Jun;154(2):103-16
references: 20668491
references: Rev Assoc Med Bras (1992). 2006 Sep-Oct;52(5):365-8
references: 17160315
references: Anticancer Res. 2009 Feb;29(2):753-61
references: 19331232
references: Semin Liver Dis. 1998;18(4):345-58
references: 9875553
references: BMC Genet. 2010 Jun 11;11:47
references: 20540773
references: Lung Cancer. 2002 Mar;35(3):221-9
references: 11844594
references: Rev Assoc Med Bras (1992). 2010 May-Jun;56(3):299-303
references: 20676536
references: J Korean Med Sci. 2006 Dec;21(6):1075-9
references: 17179690
references: Am J Hum Genet. 2005 Jun;76(6):967-86
references: 15834813
references: Carcinogenesis. 1998 Feb;19(2):275-80
references: 9498276
references: Int J Oncol. 2008 May;32(5):945-73
references: 18425322
references: Cancer Epidemiol Biomarkers Prev. 2009 Feb;18(2):541-50
references: 19190158
references: Mol Biol Rep. 2012 Feb;39(2):1055-63
references: 21590276
references: J Cancer Res Clin Oncol. 2010 Jun;136(6):945-51
references: 20012094
references: Sao Paulo Med J. 2004 Sep 2;122(5):188-94
references: 15558139