title: Genome sequences of 65 Helicobacter pylori strains isolated from asymptomatic individuals and patients with gastric cancer, peptic ulcer disease, or gastritis.
abstract: Helicobacter pylori, inhabitant of the gastric mucosa of over half of the world population, with decreasing prevalence in the U.S., has been associated with a variety of gastric pathologies. However, the majority of H. pylori-infected individuals remain asymptomatic, and negative correlations between H. pylori and allergic diseases have been reported. Comprehensive genome characterization of H. pylori populations from different human host backgrounds including healthy individuals provides the exciting potential to generate new insights into the open question whether human health outcome is associated with specific H. pylori genotypes or dependent on other environmental factors. We report the genome sequences of 65 H. pylori isolates from individuals with gastric cancer, preneoplastic lesions, peptic ulcer disease, gastritis, and from asymptomatic adults. Isolates were collected from multiple locations in North America (USA and Canada) as well as from Columbia and Japan. The availability of these H. pylori genome sequences from individuals with distinct clinical presentations provides the research community with a resource for detailed investigations into genetic elements that correlate either positively or negatively with the epidemiology, human host adaptation, and gastric pathogenesis and will aid in the characterization of strains that may favor the development of specific pathology, including gastric cancer.
authors: Department of Pediatrics, University of Maryland, Baltimore, MD, USA. tblanchard@peds.umaryland.edu
authors: Thomas G
authors: TG
authors: Blanchard
authors: Steven J
authors: SJ
authors: Czinn
authors: Pelayo
authors: P
authors: Correa
authors: Teruko
authors: T
authors: Nakazawa
authors: Monika
authors: M
authors: Keelan
authors: Lindsay
authors: L
authors: Morningstar
authors: Ivette
authors: I
authors: Santana-Cruz
authors: Ankit
authors: A
authors: Maroo
authors: Carri
authors: C
authors: McCracken
authors: Kent
authors: K
authors: Shefchek
authors: Sean
authors: S
authors: Daugherty
authors: Yang
authors: Y
authors: Song
authors: Claire M
authors: CM
authors: Fraser
authors: W Florian
authors: WF
authors: Fricke
mesh_terms: D000328:Adult; D058070:Asymptomatic Diseases; D016000:Cluster Analysis; D003105:Colombia; D004269:DNA, Bacterial / Q000737:chemistry* / Q000235:genetics*; D005756:Gastritis / Q000382:microbiology; D016680:Genome, Bacterial; D016481:Helicobacter Infections / Q000382:microbiology* / Q000473:pathology; D016480:Helicobacter pylori / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D006801:Humans; D007564:Japan; D008969:Molecular Sequence Data; D009656:North America; D010437:Peptic Ulcer / Q000382:microbiology; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D013274:Stomach Neoplasms / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial
references: Mol Microbiol. 2001 Dec;42(5):1337-48
references: 11886563
references: Lancet. 1984 Jun 16;1(8390):1311-5
references: 6145023
references: Proc Natl Acad Sci U S A. 2008 Jan 22;105(3):1003-8
references: 18192401
references: Am J Gastroenterol. 1997 Sep;92(9):1480-2
references: 9317067
references: Infect Immun. 1997 Sep;65(9):3622-30
references: 9284128
references: Intern Med. 2008;47(12):1077-83
references: 18552463
references: PLoS Pathog. 2009 Dec;5(12):e1000684
references: 19997503
references: Nature. 1997 Aug 7;388(6642):539-47
references: 9252185
references: Nature. 2007 Oct 18;449(7164):862-6
references: 17943123
references: Hum Pathol. 1993 Jun;24(6):577-83
references: 8505036
references: PLoS One. 2009 Sep 03;4(9):e6859
references: 19727398
references: JAMA. 1995 Oct 4;274(13):1064-6
references: 7563460
references: J Clin Microbiol. 1991 Jun;29(6):1128-31
references: 1864929
references: Nature. 2007 Feb 22;445(7130):915-918
references: 17287725
references: Gut. 2013 Sep;62(9):1262-9
references: 23303440
references: J Natl Cancer Inst. 1991 Dec 4;83(23):1734-9
references: 1770552
references: N Engl J Med. 1989 Dec 7;321(23):1562-6
references: 2586553
references: Lancet. 1991 Nov 9;338(8776):1175-6
references: 1682595
references: Infect Immun. 2000 Nov;68(11):6240-9
references: 11035731
references: Appl Environ Microbiol. 2012 Jul;78(14):4884-92
references: 22582056
references: N Engl J Med. 2002 Oct 10;347(15):1175-86
references: 12374879
references: Bioinformatics. 2011 Feb 1;27(3):334-42
references: 21148543
references: Nature. 1999 Jan 14;397(6715):176-80
references: 9923682
references: PLoS One. 2010 Mar 10;5(3):e9490
references: 20224823
references: Science. 2000 Feb 25;287(5457):1497-500
references: 10688800
references: Helicobacter. 2012 Feb;17(1):30-5
references: 22221613
references: BMC Genomics. 2009 Jan 05;10:3
references: 19123947
references: N Engl J Med. 1991 Oct 17;325(16):1127-31
references: 1891020
references: BMC Microbiol. 2011 May 16;11:104
references: 21575176