title: Engineered Streptomyces avermitilis host for heterologous expression of biosynthetic gene cluster for secondary metabolites.
abstract: An industrial microorganism, Streptomyces avermitilis, which is a producer of anthelmintic macrocyclic lactones, avermectins, has been constructed as a versatile model host for heterologous expression of genes encoding secondary metabolite biosynthesis. Twenty of the entire biosynthetic gene clusters for secondary metabolites were successively cloned and introduced into a versatile model host S. avermitilis SUKA17 or 22. Almost all S. avermitilis transformants carrying the entire gene cluster produced metabolites as a result of the expression of biosynthetic gene clusters introduced. A few transformants were unable to produce metabolites, but their production was restored by the expression of biosynthetic genes using an alternative promoter or the expression of a regulatory gene in the gene cluster that controls the expression of biosynthetic genes in the cluster using an alternative promoter. Production of metabolites in some transformants of the versatile host was higher than that of the original producers, and cryptic biosynthetic gene clusters in the original producer were also expressed in a versatile host.
authors: Kitasato Institute for Life Sciences, Kitasato University, 1-15-1 Kitasato, Minami-ku, Sagamihara, Kanagawa 252-0373, Japan.
authors: Mamoru
authors: M
authors: Komatsu
authors: Kyoko
authors: K
authors: Komatsu
authors: Hanae
authors: H
authors: Koiwai
authors: Yuuki
authors: Y
authors: Yamada
authors: Ikuko
authors: I
authors: Kozone
authors: Miho
authors: M
authors: Izumikawa
authors: Junko
authors: J
authors: Hashimoto
authors: Motoki
authors: M
authors: Takagi
authors: Satoshi
authors: S
authors: Omura
authors: Kazuo
authors: K
authors: Shin-ya
authors: David E
authors: DE
authors: Cane
authors: Haruo
authors: H
authors: Ikeda
mesh_terms: D003001:Cloning, Molecular / Q000379:methods; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D024861:Genetic Enhancement / Q000379:methods*; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism; D005810:Multigene Family / Q000235:genetics*; D015202:Protein Engineering / Q000379:methods*; D064210:Secondary Metabolism / Q000235:genetics*; D013045:Species Specificity; D013302:Streptomyces / Q000145:classification / Q000502:physiology*; D058615:Synthetic Biology / Q000379:methods
chemical_list: D007559:Ivermectin; C019264:avermectin
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