title: α(1,3) Fucosyltransferases IV and VII are essential for the initial recruitment of basophils in chronic allergic inflammation.
abstract: Basophils act as initiator cells for the development of IgE-mediated chronic allergic inflammation (IgE-CAI). However, detailed mechanisms of initial recruitment of basophils into the skin have yet to be clarified. Selectins mediate leukocyte capture and rolling on the vascular endothelium for extravasation. Counter-receptor activity of selectins is regulated by α(1, 3) fucosyltransferases (FTs) IV and VII. To clarify the contribution of selectin ligands regulated by FTs for initial basophil recruitment, IgE-CAI was induced in mice deficient in FT-IV and/or FT-VII genes. Although FT-IV(-/-) and FT-VII(-/-) mice exhibited comparable skin responses to wild-type mice, the FT-IV(-/-)/FT-VII(-/-) mice showed significantly impaired inflammation. Although the transfer of basophils to FcRγ(-/-) mice induced IgE-CAI, this induction was completely absent when basophils from FT-IV(-/-)/FT-VII(-/-) mice were transferred. L-selectin, but not P- and E-selectin, blocking Abs inhibited skin inflammation in vivo. P-selectin glycoprotein-1 (PSGL-1) antibody also ameliorated skin inflammation, and basophils were bound to L-selectin in a PSGL-1-dependent manner, which was regulated by FT-IV/VII. Functional PSGL-1 generated by basophil FT-IV/VII and its subsequent binding to L-selectin could be one of the essential steps required for initial basophil recruitment and the development of IgE-CAI in mice.
authors: Department of Dermatology, Tokyo Medical and Dental University, Tokyo, Japan.
authors: Kazumi
authors: K
authors: Saeki
authors: Takahiro
authors: T
authors: Satoh
authors: Hiroo
authors: H
authors: Yokozeki
mesh_terms: D000818:Animals; D001491:Basophils / Q000201:enzymology* / Q000276:immunology* / Q000637:transplantation; D001854:Bone Marrow Cells / Q000166:cytology / Q000201:enzymology / Q000276:immunology; D002465:Cell Movement / Q000276:immunology; D002478:Cells, Cultured; D002908:Chronic Disease; D017449:Dermatitis, Allergic Contact / Q000276:immunology* / Q000378:metabolism; D019040:E-Selectin / Q000276:immunology / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000276:immunology; D005647:Fucosyltransferases / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D007073:Immunoglobulin E / Q000276:immunology / Q000378:metabolism; D019041:L-Selectin / Q000276:immunology / Q000378:metabolism; D036904:Leukocyte Rolling / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008819:Mice, Nude; D019007:P-Selectin / Q000276:immunology / Q000378:metabolism; D012867:Skin / Q000276:immunology
chemical_list: D019040:E-Selectin; D019007:P-Selectin; D019041:L-Selectin; D007073:Immunoglobulin E; D005647:Fucosyltransferases; C541914:Fut4 protein, mouse; C514210:fucosyltransferase VII, mouse