title: Development of a swine model of left bundle branch block for experimental studies of cardiac resynchronization therapy.
abstract: Animal models that mimic human electrical and mechanical dyssynchrony often associated with chronic heart failure would provide an essential tool to investigate factors influencing response to cardiac resynchronization therapy. A standardized closed-chest porcine model of left bundle branch block (LBBB) was developed using 16 pigs. Radiofrequency applications were performed to induce LBBB, which was confirmed by QRS widening, a surface electrocardiogram pattern concordant with LBBB, and a prolonged activation time from endocardial. Echocardiography confirmed abnormal motion of the septum, which was not present at the baseline echocardiogram. High susceptibility of pigs to ventricular fibrillation during the endocardial ablation was overcome by applying high-rate pacing during radiofrequency applications. This is the first study to devise a closed-chest porcine model of LBBB that closely reproduces abnormalities found in patients with electrical and mechanical cardiac dyssynchrony, and provides a useful tool to investigate the basic mechanisms underlying cardiac resynchronization therapy benefits in heart failure.
authors: Department of Cardiology, Institut Clínic del Tòrax, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS) Hospital Clínic de Barcelona, Universitat de Barcelona (UB), Villarroel, 170, 08036, Barcelona, Spain. mrigol@clinic.ub.es
authors: Montserrat
authors: M
authors: Rigol
authors: Núria
authors: N
authors: Solanes
authors: Juan
authors: J
authors: Fernandez-Armenta
authors: Etelvino
authors: E
authors: Silva
authors: Adelina
authors: A
authors: Doltra
authors: Nicolas
authors: N
authors: Duchateau
authors: Aina
authors: A
authors: Barcelo
authors: Luigi
authors: L
authors: Gabrielli
authors: Bart
authors: B
authors: Bijnens
authors: Antonio
authors: A
authors: Berruezo
authors: Josep
authors: J
authors: Brugada
authors: Marta
authors: M
authors: Sitges
mesh_terms: D000200:Action Potentials; D000818:Animals; D001696:Biomechanical Phenomena; D002036:Bundle of His / Q000503:physiopathology* / Q000601:surgery; D002037:Bundle-Branch Block / Q000175:diagnosis / Q000209:etiology / Q000503:physiopathology / Q000628:therapy*; D002304:Cardiac Pacing, Artificial; D058406:Cardiac Resynchronization Therapy; D017115:Catheter Ablation; D004195:Disease Models, Animal; D004562:Electrocardiography; D013552:Swine; D013997:Time Factors; D014693:Ventricular Fibrillation / Q000209:etiology / Q000517:prevention & control
references: J Card Fail. 2010 Apr;16(4):357-66
references: 20350704
references: Eur Heart J. 2005 Jan;26(1):91-8
references: 15615805
references: J Cardiovasc Electrophysiol. 2002 Jul;13(7):691-5
references: 12139294
references: Rev Esp Cardiol. 2009 Jan;62(1):69-84
references: 19150017
references: Cryobiology. 2005 Oct;51(2):130-41
references: 16137670
references: N Engl J Med. 2005 Apr 14;352(15):1539-49
references: 15753115
references: Eur Heart J. 2007 Sep;28(17):2148-55
references: 17611254
references: Circulation. 2005 Nov 15;112(20):3174-83
references: 16286608
references: Eur Heart J. 2009 Apr;30(8):940-9
references: 19004844
references: Am J Physiol Heart Circ Physiol. 2002 Jun;282(6):H2238-44
references: 12003833
references: J Heart Lung Transplant. 2008 Apr;27(4):416-22
references: 18374878
references: J Physiol. 2007 Jan 1;578(Pt 1):85-97
references: 17110414
references: Circ Arrhythm Electrophysiol. 2009 Oct;2(5):580-7
references: 19843927
references: Circ Arrhythm Electrophysiol. 2009 Oct;2(5):571-9
references: 19843926
references: Am J Physiol Heart Circ Physiol. 2012 Jul 15;303(2):H207-15
references: 22561299
references: Lab Anim. 2004 Apr;38(2):149-57
references: 15070454
references: Prog Biophys Mol Biol. 2008 Jun-Jul;97(2-3):543-61
references: 18417196