title: Cell wall components of Leptosphaeria maculans enhance resistance of Brassica napus.
abstract: Preparations with elicitation activity were obtained from the mycelium of Leptosphaeria maculans , a fungal pathogen of oilseed rape (Brassica napus). Crude delipidated and deproteinized extract from fungal cell walls induced expression of pathogenesis related gene 1 (PR1), hydrogen peroxide accumulation, and enhanced resistance of B. napus plants toward infection by L. maculans. Elicitation activity significantly decreased after treatment of a crude extract with α- or β-glucanase. Monosaccharide composition analysis of a crude extract purified by ion-exchange chromatography revealed glucose (∼58 mol %), mannose (∼22 mol %), and galactose (∼18 mol %) as the major sugars. FT-IR and NMR spectra confirmed the presence of both carbohydrate and polypeptide components in the purified product. Correlation NMR experiments defined trisaccharide bound to O-3 of serine residue α-D-Glcp-(1→2)-β-D-Galf-(1→6)-α-D-Manp-(1→3)-L-Ser. Terminal α-D-Glcp and (1→6)-β-D-glucan were also detected. The obtained results strongly support the conclusion that these carbohydrates induce defense response in B. napus plants.
authors: Department of Biochemistry and Microbiology and ‡Department of Carbohydrates and Cereals, Institute of Chemical Technology Prague , Technická 3, 166 28 Prague 6, Czech Republic.
authors: Phuong Dinh
authors: PD
authors: Kim
authors: Vladimír
authors: V
authors: Šašek
authors: Lenka
authors: L
authors: Burketová
authors: Jana
authors: J
authors: Čopíková
authors: Andriy
authors: A
authors: Synytsya
authors: Barbora
authors: B
authors: Jindřichová
authors: Olga
authors: O
authors: Valentová
mesh_terms: D001203:Ascomycota / Q000737:chemistry* / Q000254:growth & development / Q000276:immunology; D001234:Aspergillus niger / Q000201:enzymology; D029688:Brassica napus / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D002457:Cell Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002473:Cell Wall / Q000737:chemistry*; D002622:Chemistry, Agricultural / Q000379:methods; D060467:Disease Resistance / Q000187:drug effects*; D015536:Down-Regulation; D005656:Fungal Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D005659:Fungicides, Industrial / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006026:Glycoside Hydrolases / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D006027:Glycosides / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D006868:Hydrolysis; D025282:Mycelium / Q000737:chemistry / Q000254:growth & development / Q000276:immunology; D009844:Oligosaccharides / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D010446:Peptide Fragments / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D010940:Plant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: D002457:Cell Extracts; D005656:Fungal Proteins; D005659:Fungicides, Industrial; D006027:Glycosides; D009844:Oligosaccharides; D010446:Peptide Fragments; D010940:Plant Proteins; C053376:pathogenesis-related proteins, plant; D006861:Hydrogen Peroxide; D006026:Glycoside Hydrolases