title: Potential prognostic value of intracellular cytokine detection by flow cytometry in pulmonary sarcoidosis.
abstract: In pulmonary sarcoidosis, differential cytokine production in the lungs could be related to variable prognosis of patients at different stages of disease. Twenty patients with pulmonary sarcoidosis (10 at radiographic stage I and 10 at stages II-IV), as well as 10 age-matched healthy volunteers participated in the study. A 4-colour flow cytometric technique was used to measure interferon-γ (IFN-γ), interleukin (IL)-2, tumour necrosis factor-α (TNF-α), IL-4, and IL-13 production in phorbol myristate acetate (PMA)/ionomycin-stimulated CD4+ and CD8+ T cells from bronchoalveolar lavage fluid (BALF) and peripheral blood (PB) of patients, and PB of control subjects. CD4+ T cells from patients showed higher expression of IFN-γ in BALF than in PB. Significant correlations were observed between the percentages of BALF CD4+ and CD8+ T cells expressing intracellular IFN-γ, IL-2, and TNF-α. Stage I patients had lower percentages of IFN-γ-producing CD4+ and CD8+ T cells, as well as TNF-α-producing CD8+ T cells, in BALF (but not in PB) than stage II-IV patients. A decreased TH1 and TC1 response was demonstrated in BALF of patients at stage I of disease, which could explain their anticipated better prognosis.
authors: Department of Pneumonology, Aristotle University of Thessaloniki, Georgios Papanikolaou Hospital, Thessaloniki, Greece. evigounari@yahoo.com
authors: Evdoxia
authors: E
authors: Gounari
authors: Olga
authors: O
authors: Chatzizisi
authors: Evdoxia
authors: E
authors: Diza-Mataftsi
authors: Despoina
authors: D
authors: Papakosta
authors: Theodoros
authors: T
authors: Kontakiotis
authors: Dimitris
authors: D
authors: Iakovidis
authors: Fotis
authors: F
authors: Zoglopitis
authors: Dimitris
authors: D
authors: Bougiouklis
authors: Aikaterini
authors: A
authors: Markopoulou
authors: Eva
authors: E
authors: Serasli
authors: Georgios
authors: G
authors: Kyriazis
mesh_terms: D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002469:Cell Separation; D016207:Cytokines / Q000032:analysis*; D018450:Disease Progression; D005434:Flow Cytometry; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D015759:Ionomycin / Q000378:metabolism; D008168:Lung / Q000000981:diagnostic imaging / Q000276:immunology; D008213:Lymphocyte Activation; D008875:Middle Aged; D011379:Prognosis; D011859:Radiography; D017565:Sarcoidosis, Pulmonary / Q000175:diagnosis*; D013755:Tetradecanoylphorbol Acetate / Q000378:metabolism; D058408:Th1-Th2 Balance; D055815:Young Adult
chemical_list: D015415:Biomarkers; D016207:Cytokines; D018836:Inflammation Mediators; D015759:Ionomycin; D013755:Tetradecanoylphorbol Acetate