title: Differential expression profiles in the midgut of Triatoma infestans infected with Trypanosoma cruzi.
abstract: Chagas disease, or American trypanosomiasis, is a parasitic disease caused by the protozoan Trypanosoma cruzi and is transmitted by insects from the Triatominae subfamily. To identify components involved in the protozoan-vector relationship, we constructed and analyzed cDNA libraries from RNA isolated from the midguts of uninfected and T. cruzi-infected Triatoma infestans, which are major vectors of Chagas disease. We generated approximately 440 high-quality Expressed Sequence Tags (ESTs) from each T. infestans midgut cDNA library. The sequences were grouped in 380 clusters, representing an average length of 664.78 base pairs (bp). Many clusters were not classified functionally, representing unknown transcripts. Several transcripts involved in different processes (e.g., detoxification) showed differential expression in response to T. cruzi infection. Lysozyme, cathepsin D, a nitrophorin-like protein and a putative 14 kDa protein were significantly upregulated upon infection, whereas thioredoxin reductase was downregulated. In addition, we identified several transcripts related to metabolic processes or immunity with unchanged expressions, including infestin, lipocalins and defensins. We also detected ESTs encoding juvenile hormone binding protein (JHBP), which seems to be involved in insect development and could be a target in control strategies for the vector. This work demonstrates differential gene expression upon T. cruzi infection in the midgut of T. infestans. These data expand the current knowledge regarding vector-parasite interactions for Chagas disease.
authors: Department of Biochemistry, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brazil.
authors: Diego S
authors: DS
authors: Buarque
authors: Glória R C
authors: GR
authors: Braz
authors: Rafael M
authors: RM
authors: Martins
authors: Anita M
authors: AM
authors: Tanaka-Azevedo
authors: Cícera M
authors: CM
authors: Gomes
authors: Felipe A A
authors: FA
authors: Oliveira
authors: Sergio
authors: S
authors: Schenkman
authors: Aparecida S
authors: AS
authors: Tanaka
mesh_terms: D000818:Animals; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling; D006790:Host-Parasite Interactions / Q000235:genetics; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000469:parasitology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014224:Triatoma / Q000235:genetics* / Q000469:parasitology*; D014349:Trypanosoma cruzi / Q000502:physiology*; D015854:Up-Regulation
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger
references: Infect Genet Evol. 2010 Mar;10(2):184-91
references: 19900580
references: Subcell Biochem. 2008;47:140-54
references: 18512348
references: Insect Biochem Mol Biol. 2011 Oct;41(10):823-31
references: 21736942
references: Insect Biochem Mol Biol. 2008 Feb;38(2):213-32
references: 18207082
references: Nucleic Acids Res. 2000 Jan 1;28(1):263-6
references: 10592242
references: J Insect Physiol. 2008 Mar;54(3):593-603
references: 18258253
references: Genome Res. 1999 Sep;9(9):868-77
references: 10508846
references: PLoS Biol. 2010 Feb 23;8(2):e1000313
references: 20186266
references: Dev Comp Immunol. 2007;31(2):109-20
references: 16824597
references: Biochem Biophys Res Commun. 2011 Sep 23;413(2):241-7
references: 21875578
references: Insect Mol Biol. 2011 Dec;20(6):775-86
references: 21906194
references: BMC Bioinformatics. 2003 Sep 11;4:41
references: 12969510
references: FEMS Microbiol Lett. 2002 Jan 2;206(1):9-18
references: 11786250
references: BMC Genomics. 2011 Sep 09;12:446
references: 21906285
references: Biochim Biophys Acta. 2000 Oct 18;1482(1-2):9-24
references: 11058743
references: Insect Biochem Mol Biol. 2012 Apr;42(4):240-50
references: 22210150
references: Antioxid Redox Signal. 2013 Sep 1;19(7):723-34
references: 22458250
references: Curr Protoc Bioinformatics. 2007 Jun;Chapter 4:Unit 4.3
references: 18428791
references: Insect Biochem Mol Biol. 2006 Jul;36(7):547-60
references: 16835020
references: Nucleic Acids Res. 2002 Jan 1;30(1):242-4
references: 11752305
references: J Proteomics. 2011 Aug 24;74(9):1693-700
references: 21601023
references: Int J Parasitol. 2007 Oct;37(12):1351-8
references: 17575982
references: FEBS Lett. 2004 Nov 19;577(3):512-6
references: 15556638
references: Vector Borne Zoonotic Dis. 2011 Jul;11(7):845-7
references: 20925526
references: Nat Genet. 2000 May;25(1):25-9
references: 10802651
references: Am J Trop Med Hyg. 1998 Jul;59(1):166-70
references: 9684647
references: Insect Biochem Mol Biol. 2006 Apr;36(4):322-35
references: 16551546
references: Insect Biochem Mol Biol. 2002 Sep;32(9):991-7
references: 12213235
references: Lancet. 2010 Apr 17;375(9723):1388-402
references: 20399979
references: IUBMB Life. 2002 Feb;53(2):121-3
references: 12049194
references: Exp Parasitol. 2006 Feb;112(2):130-3
references: 16288741
references: Insect Biochem Mol Biol. 2007 May;37(5):440-52
references: 17456439
references: Proc Natl Acad Sci U S A. 1997 Oct 28;94(22):11845-50
references: 9342325
references: Nucleic Acids Res. 2002 Jan 1;30(1):281-3
references: 11752315
references: Appl Environ Microbiol. 2008 Mar;74(5):1453-63
references: 18192407
references: Exp Parasitol. 2011 Dec;129(4):362-7
references: 21963772
references: Insect Biochem Mol Biol. 2008 Sep;38(9):841-52
references: 18694828
references: Infect Immun. 2008 Dec;76(12):5543-52
references: 18794282
references: Insect Biochem Mol Biol. 2007 Jul;37(7):702-12
references: 17550826
references: J Invertebr Pathol. 1995 May;65(3):261-8
references: 7745280
references: Front Biosci. 2008 May 01;13:5406-20
references: 18508595
references: Bioinformatics. 2009 May 1;25(9):1195-6
references: 19318425
references: Methods Enzymol. 1996;266:460-80
references: 8743700
references: J Parasitol Res. 2011;2011:174614
references: 22007287
references: Insect Biochem Mol Biol. 2003 Apr;33(4):439-47
references: 12650692
references: Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
references: 9254694
references: Methods. 2001 Dec;25(4):402-8
references: 11846609
references: Arch Insect Biochem Physiol. 2003 Jul;53(3):134-45
references: 12811767
references: Parasit Vectors. 2011 Mar 18;4:39
references: 21418565