title: Comparison of gene expression profiles between pansensitive and multidrug-resistant strains of Mycobacterium tuberculosis.
abstract: Mycobacterium tuberculosis has developed resistance to anti-tuberculosis first-line drugs. Multidrug-resistant strains complicate the control of tuberculosis and have converted it into a worldwide public health problem. Mutational studies of target genes have tried to envisage the resistance in clinical isolates; however, detection of these mutations in some cases is not sufficient to identify drug resistance, suggesting that other mechanisms are involved. Therefore, the identification of new markers of susceptibility or resistance to first-line drugs could contribute (1) to specifically diagnose the type of M. tuberculosis strain and prescribe an appropriate therapy, and (2) to elucidate the mechanisms of resistance in multidrug-resistant strains. In order to identify specific genes related to resistance in M. tuberculosis, we compared the gene expression profiles between the pansensitive H37Rv strain and a clinical CIBIN:UMF:15:99 multidrug-resistant isolate using microarray analysis. Quantitative real-time PCR confirmed that in the clinical multidrug-resistant isolate, the esxG, esxH, rpsA, esxI, and rpmI genes were upregulated, while the lipF, groES, and narG genes were downregulated. The modified genes could be involved in the mechanisms of resistance to first-line drugs in M. tuberculosis and could contribute to increased efficiency in molecular diagnosis approaches of infections with drug-resistant strains.
authors: Departamento de Biología Molecular, Centro de Investigación Biomédica del Noreste, Instituto Mexicano del Seguro Social, Calle 2 de abril 501, Col. Independencia, 64720, Monterrey, Nuevo León, Mexico.
authors: K
authors: K
authors: Peñuelas-Urquides
authors: L
authors: L
authors: González-Escalante
authors: L
authors: L
authors: Villarreal-Treviño
authors: B
authors: B
authors: Silva-Ramírez
authors: D J
authors: DJ
authors: Gutiérrez-Fuentes
authors: R
authors: R
authors: Mojica-Espinosa
authors: C
authors: C
authors: Rangel-Escareño
authors: L
authors: L
authors: Uribe-Figueroa
authors: G M
authors: GM
authors: Molina-Salinas
authors: J
authors: J
authors: Dávila-Velderrain
authors: F
authors: F
authors: Castorena-Torres
authors: M
authors: M
authors: Bermúdez de León
authors: S
authors: S
authors: Said-Fernández
mesh_terms: D024901:Drug Resistance, Multiple, Bacterial; D005798:Genes, Bacterial; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D059467:Transcriptome
references: Arch Med Res. 2006 Jan;37(1):45-9
references: 16314185
references: Tuberculosis (Edinb). 2004;84(3-4):263-74
references: 15207496
references: PLoS Pathog. 2010 Jul 08;6(7):e1000988
references: 20628579
references: Int J Tuberc Lung Dis. 2011 Feb;15(2):205-10, i
references: 21219682
references: Genome Biol. 2007;8(12):R265
references: 18078514
references: Infect Immun. 2003 Mar;71(3):1379-88
references: 12595455
references: BMC Microbiol. 2009 Mar 04;9:48
references: 19257911
references: Antimicrob Agents Chemother. 2003 Sep;47(9):2903-13
references: 12936993
references: Jpn J Infect Dis. 2008 Jul;61(4):264-8
references: 18653966
references: Arch Biochem Biophys. 2004 Apr 15;424(2):226-34
references: 15047195
references: PLoS Pathog. 2011 Nov;7(11):e1002342
references: 22072964
references: J Clin Microbiol. 1993 Feb;31(2):406-9
references: 8381814
references: J Infect Dev Ctries. 2009 May 01;3(4):278-84
references: 19759491
references: Braz J Microbiol. 2013 May 31;44(1):287-9
references: 24159318
references: Infect Immun. 2002 Jan;70(1):286-91
references: 11748194
references: PLoS Pathog. 2011 Sep;7(9):e1002262
references: 21949656
references: J Bacteriol. 2001 Dec;183(24):7371-80
references: 11717296
references: Nucleic Acids Res. 2003 Feb 15;31(4):e15
references: 12582260
references: J Clin Microbiol. 1997 Apr;35(4):907-14
references: 9157152
references: J Biol Chem. 2011 Aug 26;286(34):29993-30002
references: 21730061
references: Clin Microbiol Infect. 2009 Jan;15 Suppl 1:66-8
references: 19220360
references: EURASIP J Bioinform Syst Biol. 2007;:23054
references: 18253472
references: BMC Microbiol. 2007 May 14;7:37
references: 17501996
references: Tuberculosis (Edinb). 2005 Sep-Nov;85(5-6):385-94
references: 16253564
references: Microbiology (Reading). 2005 Jan;151(Pt 1):5-14
references: 15632420
references: J Clin Microbiol. 2009 Jun;47(6):1767-72
references: 19386845
references: Nat Med. 1997 May;3(5):567-70
references: 9142129
references: Methods. 2001 Dec;25(4):402-8
references: 11846609
references: Antimicrob Agents Chemother. 2011 Dec;55(12):5654-9
references: 21911575
references: Toxicol In Vitro. 2008 Mar;22(2):411-21
references: 18494146
references: Science. 2011 Sep 16;333(6049):1630-2
references: 21835980
references: Stat Appl Genet Mol Biol. 2004;3:Article3
references: 16646809
references: Respir Res. 2001;2(3):164-8
references: 11686881
references: BMC Genomics. 2007 Mar 30;8:88
references: 17397532
references: J Clin Microbiol. 2005 Aug;43(8):4010-4
references: 16081943