title: [Effect of different intensities of electroacupuncture stimulation on expression of SOCS-3 and PPAR-gamma mRNA in adipose tissues of obesity rats].
abstract: OBJECTIVE
  To observe the effect of electroacupuncture (EA) on the expression of suppressor of cytokine signaling 3 (SOCS-3) and peroxisome proliferator-activated receptor (PPAR)-gamma genes in the epididymis adipose tissue of obesity rats so as to explore its mechanism underlying weight reduction.
  
  
  METHODS
  SD rats were randomized into normal control, obesity model, EA-5 mA and EA-1 mA groups (10 rats/group). EA stimulation (6 Hz/20 Hz, 5 mA or 1 mA) was delivered to the bilateral "Zusanli" (ST 36) and "Sanyinjiao" (SP 6) for 15 min, once everyday for two weeks. The expression of SOCS-3 mRNA and PPAR-gamma mRNA in the epididymis adipose tissue was detected by reverse transcriptase polymerase chain reaction.
  
  
  RESULTS
  Compared with the normal group, the body weight and expression of SOCS-3 and PPAR-gamma mRNA in the model group were up-regulated obviously (P < 0.01). Compared with the model group, the body weight and expression of SOCS-3 and PPAR-gamma mRNA were significantly decreased in the EA group, with the effects of EA-5 mA being better than those of EA-1 mA.
  
  
  CONCLUSIONS
  EA stimulation of ST 36-SP 6 can reduce body weight in rats with simple obesity, and the effect of higher intensity of EA is apparently better. Accordingly, EA intervention induces down-regulation of SOCS-3 and PPAR-gamma mRNA expression in the epididymis adipose tissue.
authors: College of Chinese Medicine, Chongqing Medical University, Chongqing 400016, China. 18725657505@163.com
authors: Jie
authors: J
authors: Gao
authors: Cheng-Lin
authors: CL
authors: Tang
authors: Ren-Jian
authors: RJ
authors: Liu
authors: Xiao-Lin
authors: XL
authors: Chen
authors: Hui
authors: H
authors: Xie
authors: Yi-Xuan
authors: YX
authors: Hou
authors: Min
authors: M
authors: Yu
authors: Zu-Li
authors: ZL
authors: Liu
mesh_terms: D015669:Acupuncture Points; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D004195:Disease Models, Animal; D015671:Electroacupuncture / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D008297:Male; D009765:Obesity / Q000235:genetics* / Q000378:metabolism; D047495:PPAR gamma / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D047495:PPAR gamma; D012333:RNA, Messenger; C491410:Socs3 protein, rat; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins