title: Two novel class II hydrophobins from Trichoderma spp. stimulate enzymatic hydrolysis of poly(ethylene terephthalate) when expressed as fusion proteins.
abstract: Poly(ethylene terephthalate) (PET) can be functionalized and/or recycled via hydrolysis by microbial cutinases. The rate of hydrolysis is however low. Here, we tested whether hydrophobins (HFBs), small secreted fungal proteins containing eight positionally conserved cysteine residues, are able to enhance the rate of enzymatic hydrolysis of PET. Species of the fungal genus Trichoderma have the most proliferated arsenal of class II hydrophobin-encoding genes among fungi. To this end, we studied two novel class II HFBs (HFB4 and HFB7) of Trichoderma. HFB4 and HFB7, produced in Escherichia coli as fusions to the C terminus of glutathione S-transferase, exhibited subtle structural differences reflected in hydrophobicity plots that correlated with unequal hydrophobicity and hydrophily, respectively, of particular amino acid residues. Both proteins exhibited a dosage-dependent stimulation effect on PET hydrolysis by cutinase from Humicola insolens, with HFB4 displaying an adsorption isotherm-like behavior, whereas HFB7 was active only at very low concentrations and was inhibitory at higher concentrations. We conclude that class II HFBs can stimulate the activity of cutinases on PET, but individual HFBs can display different properties. The present findings suggest that hydrophobins can be used in the enzymatic hydrolysis of aromatic-aliphatic polyesters such as PET.
authors: Austrian Centre of Industrial Biotechnology, Graz, Austria.
authors: Liliana
authors: L
authors: Espino-Rammer
authors: Doris
authors: D
authors: Ribitsch
authors: Agnieszka
authors: A
authors: Przylucka
authors: Annemarie
authors: A
authors: Marold
authors: Katrin J
authors: KJ
authors: Greimel
authors: Enrique
authors: E
authors: Herrero Acero
authors: Georg M
authors: GM
authors: Guebitz
authors: Christian P
authors: CP
authors: Kubicek
authors: Irina S
authors: IS
authors: Druzhinina
mesh_terms: D000595:Amino Acid Sequence; D001203:Ascomycota / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005982:Glutathione Transferase / Q000378:metabolism; D006868:Hydrolysis; D010802:Phylogeny; D011092:Polyethylene Glycols / Q000737:chemistry / Q000378:metabolism*; D011093:Polyethylene Terephthalates; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D016415:Sequence Alignment; D013045:Species Specificity; D014242:Trichoderma / Q000737:chemistry / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D011093:Polyethylene Terephthalates; D011993:Recombinant Fusion Proteins; D011092:Polyethylene Glycols; D004247:DNA; D005982:Glutathione Transferase; D002265:Carboxylic Ester Hydrolases; C019952:cutinase
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