title: Innate and adaptive immune mechanisms are effectively induced in ileal Peyer's patches of Salmonella typhimurium infected pigs.
abstract: In this report we employed laser-capture microdissection (LCM) coupled to qPCR technology and bioinformatic analysis to characterize, for the first time, the response of Peyer's patches (PP) from orally infected animals to Salmonella typhimurium, in a model of non-typhoidal salmonellosis. Pathogen was highly found in the cytoplasm of phagocytes in PP and differential gene expression analysis indicated an up-regulation of proinflammatory molecules, establishment of a Th1 driven response and triggering of DC and T-cell activity. Furthermore, predictions by bioinformatic analysis pointed to an activation of processes regarding stimulation and maturation of DC, influx of leukocytes in tissue and T lymphocytes priming and differentiation. In short, the approach used in this study proved to be a promising strategy to explore infectious processes. Indeed, it revealed an effective induction of innate and adaptive immune mechanisms in swine PP which appear to be distinct from those observed in mesenteric lymph nodes and closely related to response of gut mucosa.
authors: Grupo de Genómica y Mejora Animal, Departamento de Genética, Facultad de Veterinaria, Universidad de Córdoba, Campus de Rabanales, Edificio Gregor Mendel C5, 14071 Córdoba, Spain.
authors: Rodrigo Prado
authors: RP
authors: Martins
authors: Valentina
authors: V
authors: Lorenzi
authors: Cristina
authors: C
authors: Arce
authors: Concepción
authors: C
authors: Lucena
authors: Ana
authors: A
authors: Carvajal
authors: Juan José
authors: JJ
authors: Garrido
mesh_terms: D056704:Adaptive Immunity; D000818:Animals; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology / Q000821:virology; D005786:Gene Expression Regulation; D007082:Ileum / Q000276:immunology* / Q000821:virology; D007113:Immunity, Innate; D061346:Laser Capture Microdissection; D008213:Lymphocyte Activation; D010581:Peyer's Patches / Q000276:immunology* / Q000821:virology; D010586:Phagocytes / Q000276:immunology / Q000821:virology; D012481:Salmonella Infections, Animal / Q000235:genetics / Q000276:immunology* / Q000821:virology; D012486:Salmonella typhimurium / Q000276:immunology; D013552:Swine; D013601:T-Lymphocytes / Q000276:immunology / Q000821:virology; D051193:Toll-Like Receptors / Q000235:genetics / Q000276:immunology
chemical_list: D016207:Cytokines; D051193:Toll-Like Receptors