title: The response profiles of HSPA12A and TCTP from Mytilus galloprovincialis to pathogen and cadmium challenge.
abstract: Heat shock 70 kDa protein 12A (HSPA12A) is an atypical member of HSP70 family, and the translationally controlled tumor protein (TCTP) is a novel HSP with chaperone-like activity. They are both involved in protecting organisms against various stressors. In the present study, the cDNAs of HSPA12A and TCTP (called MgHSPA12A and MgTCTP) were identified from Mytilus galloprovincialis by RACE approaches. The full-length cDNA of MgHSPA12A and MgTCTP encoded a peptide of 491 and 171 amino acids, respectively. Real-time PCR was employed to analyze the tissue distribution and temporal expression of these two genes after bacterial challenge and cadmium (Cd) exposure. It was found that the transcripts of MgHSPA12A and MgTCTP were dominantly expressed in gonad and muscle, respectively. The expression level of MgTCTP at 48 h post Vibrio anguillarum challenge was detected to be significantly up-regulated in hepatopancreas (P < 0.05). As concerned to Cd exposure, 2.0-fold increase of MgHSPA12A expression compared to that of the control was observed at 48 h in 5 μg/L Cd(2+)-treated group, while the expression levels of MgTCTP were significantly decreased after exposed to both 5 and 50 μg/L Cd(2+) for 24 h and 96 h. These results suggested the potential involvement of MgHSPA12A and MgTCTP in the mediation of the immune responses and environmental stress in mussels.
authors: Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China.
authors: Liping
authors: L
authors: You
authors: Xuanxuan
authors: X
authors: Ning
authors: Feng
authors: F
authors: Liu
authors: Jianmin
authors: J
authors: Zhao
authors: Qing
authors: Q
authors: Wang
authors: Huifeng
authors: H
authors: Wu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002104:Cadmium / Q000633:toxicity; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D049878:Mytilus / Q000187:drug effects / Q000235:genetics* / Q000276:immunology* / Q000382:microbiology; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment / Q000662:veterinary; D000090324:Tumor Protein, Translationally-Controlled 1; D014733:Vibrio / Q000502:physiology; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D014408:Biomarkers, Tumor; D018076:DNA, Complementary; D018840:HSP70 Heat-Shock Proteins; D012333:RNA, Messenger; D000090324:Tumor Protein, Translationally-Controlled 1; D014874:Water Pollutants, Chemical; D002104:Cadmium
keywords: Cadmium; Gene expression; Heat shock proteins; Mytilus galloprovincialis; Vibrio anguillarum