title: Analysis of magnetotactic behavior by swimming assay.
abstract: Prokaryotic organelles called magnetosomes allow magnetotactic bacteria to navigate along geomagnetic field lines. In this study, we modified a swimming assay commonly used to assess bacterial motility to develop a new method of assessing magnetotactic motility. By this method, the swimming assay was performed in an artificial magnetic field. Magnetotactic bacteria formed a wedge-shaped swimming halo that elongated parallel to the magnetic field. Magnetotactic motility was qualitatively assessed by comparing halo shapes. We termed this method the magnetic swimming assay. On the magnetic swimming assay, the mamK deletion strain formed a shorter halo than the wild type, indicating that the assay sensitively detects differences in magnetotactic motility. Moreover, we isolated two spontaneous magnetotactic motility mutants using magnetic swimming plates. Our findings indicate that the magnetic swimming assay is a useful method for the sensitive analysis of magnetotaxis phenotypes and mutant screening.
authors: Department of Life Sciences, Graduate School of Natural Science and Technology, Kanazawa University, Kakuma-machi, Kanazawa, Japan.
authors: Shoutaro
authors: S
authors: Sakaguchi
authors: Azuma
authors: A
authors: Taoka
authors: Yoshihiro
authors: Y
authors: Fukumori
mesh_terms: D007501:Iron / Q000378:metabolism; D060526:Magnetic Fields; D042201:Magnetospirillum / Q000166:cytology* / Q000235:genetics / Q000378:metabolism; D009068:Movement; D009154:Mutation; D010641:Phenotype; D013550:Swimming
chemical_list: D007501:Iron