title: The UBXN-2/p37/p47 adaptors of CDC-48/p97 regulate mitosis by limiting the centrosomal recruitment of Aurora A.
abstract: Coordination of cell cycle events in space and time is crucial to achieve a successful cell division. Here, we demonstrate that UBXN-2, a substrate adaptor of the AAA ATPase Cdc48/p97, is required to coordinate centrosome maturation timing with mitosis. In UBXN-2-depleted Caenorhabditis elegans embryos, centrosomes recruited more AIR-1 (Aurora A), matured precociously, and alignment of the mitotic spindle with the axis of polarity was impaired. UBXN-2 and CDC-48 coimmunoprecipitated with AIR-1 and the spindle alignment defect was partially rescued by co-depleting AIR-1, indicating that UBXN-2 controls these processes via AIR-1. Similarly, depletion in human cells of the UBXN-2 orthologues p37/p47 resulted in an accumulation of Aurora A at centrosomes and a delay in centrosome separation. The latter defect was also rescued by inhibiting Aurora A. We therefore postulate that the role of this adaptor in cell cycle regulation is conserved.
authors: Department of Physiology and Metabolism, Faculty of Medicine, University of Geneva, 1211 Geneva, Switzerland.
authors: Elsa
authors: E
authors: Kress
authors: Françoise
authors: F
authors: Schwager
authors: René
authors: R
authors: Holtackers
authors: Jonas
authors: J
authors: Seiler
authors: François
authors: F
authors: Prodon
authors: Esther
authors: E
authors: Zanin
authors: Annika
authors: A
authors: Eiteneuer
authors: Mika
authors: M
authors: Toya
authors: Asako
authors: A
authors: Sugimoto
authors: Hemmo
authors: H
authors: Meyer
authors: Patrick
authors: P
authors: Meraldi
authors: Monica
authors: M
authors: Gotta
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism* / Q000502:physiology; D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D064096:Aurora Kinase A; D064127:Aurora Kinases; D017173:Caenorhabditis elegans / Q000166:cytology / Q000235:genetics; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism* / Q000502:physiology; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002460:Cell Line; D018385:Centrosome / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D008938:Mitosis; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D034622:RNA Interference; D000074405:Valosin Containing Protein
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D029742:Caenorhabditis elegans Proteins; D018797:Cell Cycle Proteins; C582208:UBXN-2 protein, C elegans; C553975:p37 protein, human; D049452:Green Fluorescent Proteins; D064096:Aurora Kinase A; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases; C576939:air-1 protein, C elegans; D000251:Adenosine Triphosphatases; D000074405:Valosin Containing Protein
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