title: Gene expression analysis of peripheral blood cells reveals Toll-like receptor pathway deregulation in colorectal cancer.
abstract: Colorectal cancer is the leading cause of cancer-related deaths worldwide. The disease is curable when detected at an early stage. However, the compliance rate with current screening recommendations remains poor. An accurate, minimally invasive blood test that has the potential for greater patient compliance would be a welcome addition to the current methods. Recent data have shown that gene expression profile of peripheral blood cells can reflect disease states and thus have diagnostic value. In this study, genome-wide gene expression profiling of peripheral blood cells from 20 healthy controls and 20 colorectal cancer patients were performed using PAXgene™ technology and Affymetrix GeneChip® microarrays. We identified a list of 1,469 genes that were differentially expressed between the healthy controls and cancer patients. Gene annotation and functional enrichment analysis revealed that those genes are mainly related to immune functions. Particularly, a set of genes belonging to the Toll-Like Receptor pathways were up-regulated in the colorectal cancer patients. These findings provide a new understanding of blood gene expression profile in colorectal cancer. Our result may serve as the basis for further development of blood biomarkers for the diagnosis and treatment of colorectal cancer.
authors: Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China.
authors: Ye
authors: Y
authors: Xu
authors: Qinghua
authors: Q
authors: Xu
authors: Li
authors: L
authors: Yang
authors: Fang
authors: F
authors: Liu
authors: Xun
authors: X
authors: Ye
authors: Fei
authors: F
authors: Wu
authors: Shujuan
authors: S
authors: Ni
authors: Cong
authors: C
authors: Tan
authors: Guoxiang
authors: G
authors: Cai
authors: Xia
authors: X
authors: Meng
authors: Sanjun
authors: S
authors: Cai
authors: Xiang
authors: X
authors: Du
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics; D051193:Toll-Like Receptors / Q000378:metabolism*; D059467:Transcriptome
chemical_list: D051193:Toll-Like Receptors
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