title: Use of the mitochondria toxicity assay for quantifying the viable cell density of microencapsulated jurkat cells.
abstract: The mitochondria toxicity assay (MTT assay) is an established method for monitoring cell viability based on mitochondrial activity. Here the MTT assay is proposed for the in situ quantification of the living cell density of microencapsulated Jurkat cells. Three systems were used to encapsulate the cells, namely a membrane consisting of an interpenetrating polyelectrolyte network of sodium cellulose sulphate/poly(diallyldimethylammonium chloride) (NaCS/PDADMAC), a calcium alginate hydrogel covered with poly(L-lysine) (Ca-alg-PLL), and a novel calcium alginate-poly(ethylene glycol) hybrid material (Ca-alg-PEG). MTT results were correlated to data obtained by the trypan blue exclusion assay after release of the cells from the NaCS/PDADMAC and Ca-alg-PLL capsules, while a resazurin-based assay was used for comparison in case of the Ca-alg-PEG material. Analysis by MTT assay allows quick and reliable determination of viable cell densities of encapsulated cells independent of the capsule material. The assay is highly reproducible with inter-assay relative standard deviations below 10%.
authors: Chair for Process Biotechnology, University of Bayreuth, Bayreuth, Germany.
authors: M
authors: M
authors: Werner
authors: K
authors: K
authors: Biss
authors: V
authors: V
authors: Jérôme
authors: F
authors: F
authors: Hilbrig
authors: R
authors: R
authors: Freitag
authors: K
authors: K
authors: Zambrano
authors: H
authors: H
authors: Hübner
authors: R
authors: R
authors: Buchholz
authors: R
authors: R
authors: Mahou
authors: C
authors: C
authors: Wandrey
mesh_terms: D000464:Alginates / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002482:Cellulose / Q000031:analogs & derivatives* / Q000494:pharmacology; D020723:Glucuronic Acid / Q000494:pharmacology; D006603:Hexuronic Acids / Q000494:pharmacology; D006801:Humans; D019169:Jurkat Cells; D008928:Mitochondria / Q000187:drug effects*; D011092:Polyethylene Glycols / Q000494:pharmacology*; D011095:Polyethylenes / Q000494:pharmacology*; D000644:Quaternary Ammonium Compounds / Q000494:pharmacology*; D013329:Structure-Activity Relationship
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D011095:Polyethylenes; D000644:Quaternary Ammonium Compounds; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride; D011092:Polyethylene Glycols; D020723:Glucuronic Acid; D002482:Cellulose; C008881:phosphocellulose
keywords: MTT assay; cell viability; encapsulated cells; resazurin