title: 25-Hydroxyvitamin D(3)-loaded PLA microspheres: in vitro characterization and application in diabetic periodontitis models.
abstract: This study aimed at the preparation of a sustained-release 25-hydroxyvitamin D(3) (25OHD) treatment for diabetic periodontitis, a known complication of diabetes. 25OHD-loaded polylactic acid (PLA) microspheres were prepared using oil-in-water emulsion-solvent evaporation method. The prepared microspheres exhibited intact surfaces, with average sizes ranging from 42.3 to 119.4 μm. The encapsulation efficiency ranged from 79.2% (w/w) to 88.5% (w/w), and the drug content was between 15.8% (w/w) and 17.8% (w/w). Drug release from the produced microspheres followed a near-to-zero-order release pattern and lasted over 10 weeks. In an in vitro model of diabetic periodontitis, the abnormal morphological changes and the decrease in the cell viability of bone marrow stromal cells could be effectively attenuated after the 25OHD-loaded microsphere application. Additionally, in a rat model of diabetic periodontitis, alveolar bone loss was inhibited and osteoid formation in the periodontium was promoted upon 25OHD-loaded microsphere treatment. In conclusion, 25OHD-loaded PLA microspheres may provide an effective approach for the treatment of this disease.
authors: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, 14 3rd Section S Renmin Road, Chengdu 610041, China.
authors: Hao
authors: H
authors: Li
authors: Qi
authors: Q
authors: Wang
authors: Yu
authors: Y
authors: Xiao
authors: Chongyun
authors: C
authors: Bao
authors: Wei
authors: W
authors: Li
mesh_terms: D016301:Alveolar Bone Loss / Q000188:drug therapy / Q000382:microbiology / Q000473:pathology; D000818:Animals; D002112:Calcifediol / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002626:Chemistry, Pharmaceutical; D003692:Delayed-Action Preparations; D003921:Diabetes Mellitus, Experimental / Q000150:complications*; D004337:Drug Carriers; D004655:Emulsions; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000737:chemistry*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000187:drug effects / Q000382:microbiology / Q000473:pathology; D008863:Microspheres; D009821:Oils / Q000737:chemistry; D010316:Particle Size; D010518:Periodontitis / Q000188:drug therapy* / Q000382:microbiology / Q000473:pathology; D010519:Periodontium / Q000187:drug effects / Q000382:microbiology / Q000473:pathology; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar; D012995:Solubility; D013499:Surface Properties; D013678:Technology, Pharmaceutical / Q000379:methods; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D003692:Delayed-Action Preparations; D004337:Drug Carriers; D004655:Emulsions; D009821:Oils; D011091:Polyesters; D011108:Polymers; D014867:Water; D019344:Lactic Acid; C033616:poly(lactide); D002112:Calcifediol
references: J Control Release. 2005 Oct 3;107(2):310-9
references: 16255081
references: Microb Pathog. 2008 Sep;45(3):173-80
references: 18573331
references: J Control Release. 2013 Jan 10;165(1):29-37
references: 23103455
references: J Clin Periodontol. 2011 Mar;38 Suppl 11:142-58
references: 21323711
references: J Periodontol. 2012 May;83(5):664-71
references: 21966942
references: J Diabetes Complications. 2010 Sep-Oct;24(5):334-44
references: 19628413
references: Antimicrob Agents Chemother. 1999 May;43(5):1144-51
references: 10223927
references: Clin Cases Miner Bone Metab. 2010 May;7(2):126-9
references: 22460017
references: J Bone Miner Res. 2011 May;26(5):1145-53
references: 21542014
references: AAPS PharmSciTech. 2008;9(4):1119-29
references: 18989787
references: Cell Immunol. 2011;271(1):173-9
references: 21788014
references: Steroids. 2013 Feb;78(2):115-20
references: 23138030
references: J Periodontol. 2011 Jan;82(1):25-32
references: 20809866
references: Trials. 2012 Feb 08;13:15
references: 22316314
references: J Microencapsul. 2004 Aug;21(5):539-52
references: 15513760
references: Int J Endocrinol. 2012;2012:947187
references: 23251156
references: J Periodontol. 2012 May;83(5):644-52
references: 21966943
references: Int J Pharm. 2001 Jan 16;212(2):161-9
references: 11165073
references: J Cell Biochem. 2010 Aug 15;110(6):1324-33
references: 20564226
references: Biomaterials. 2011 May;32(15):3750-63
references: 21396709
references: J Clin Periodontol. 2012 Apr;39(4):350-6
references: 22296704
references: J Periodontol. 2008 May;79(5):774-83
references: 18454655
references: J Pharmacol Sci. 2012;119(3):205-13
references: 22786584
references: Antimicrob Agents Chemother. 1998 Oct;42(10):2682-9
references: 9756777
references: Microbiology (Reading). 2009 Oct;155(Pt 10):3238-3246
references: 19608609
references: Mol Oral Microbiol. 2012 Feb;27(1):23-33
references: 22230463
references: Mol Immunol. 2012 Aug;52(1):38-49
references: 22580404
references: Acta Biomater. 2008 Jul;4(4):1046-56
references: 18342589
references: J Pharmacol Exp Ther. 2012 Nov;343(2):401-12
references: 22892342
references: Infect Immun. 2012 Jun;80(6):2247-56
references: 22451521
references: FASEB J. 2012 Apr;26(4):1423-30
references: 22179526
references: Bone. 2012 Jan;50(1):276-88
references: 22086137
references: AAPS PharmSciTech. 2010 Jun;11(2):859-69
references: 20490959
references: J Periodontol. 2012 May;83(5):581-9
references: 21942791
references: Drug Dev Ind Pharm. 1998 Sep;24(9):819-25
references: 9876532
references: Cardiology. 2013;124(1):11-7
references: 23257889
references: Br J Nutr. 2010 Feb;103(4):549-55
references: 19781131
references: Periodontol 2000. 2007;44:127-53
references: 17474930
references: Stem Cells Dev. 2011 Mar;20(3):465-74
references: 20939707
references: J Periodontol. 2009 Nov;80(11):1815-23
references: 19905951
references: Am J Physiol Cell Physiol. 2012 Sep 15;303(6):C592-4
references: 22785118
references: Biochem Cell Biol. 2006 Feb;84(1):93-101
references: 16462893
references: Pharmazie. 2011 Sep;66(9):654-61
references: 22026119
references: J Periodontol. 2008 Jul;79(7):1208-16
references: 18597603
references: AAPS PharmSciTech. 2011 Mar;12(1):1-9
references: 21152999
references: Infect Immun. 2005 Apr;73(4):2327-35
references: 15784578
references: Adv Drug Deliv Rev. 1997 Oct 13;28(1):5-24
references: 10837562
references: Atherosclerosis. 2013 Mar;227(1):140-6
references: 23298824