title: Crocin restores hypotensive effect of subchronic administration of diazinon in rats.
abstract: OBJECTIVE
  In this study, the effects of crocin against subchronic toxicity of diazinon (DZN) on systolic blood pressure (SBP) and heart rate (HR) were evaluated in rats.
  
  
  METHODS
  Rats were equally divided into 7 groups; control (corn oil), DZN (15 mg/kg), crocin (each group received 12.5, 25 or 50 mg/kg crocin plus DZN), vitamin E (200 IU/kg plus DZN) and crocin (50 mg/kg) treated groups. Rats were given DZN via gavage once a day for 4 weeks. Vitamin E (three times per week) and crocin (once a day) were intraperitoneally injected to rats for 4 weeks. Plasma cholinesterase activity (Elman method), malondealdehyde (MDA) levels in the aortic tissue (Thiobarbituric acid reactive substances or TBARS method); SBP and HR (tail cuff method) were evaluated at the end of 4th week.
  
  
  RESULTS
  A significant decrease in cholinesterase activity was observed in DZN group (P< 0.001). Crocin did not show any effects on cholinesterase activity. DZN increased MDA levels in aortic tissue (P< 0.001) in comparison with control group. Crocin and vitamin E plus DZN decreased MDA elevation induced by DZN in aortic tissue. DZN significantly reduced SBP (P< 0.01) and increased HR (P< 0.001) in comparison with control. Concurrent administration of crocin and DZN, improved the reduction of SBP and the elevation of HR induced by DZN in rat. Crocin alone did not have any effect on SBP and HR.
  
  
  CONCLUSIONS
  This study showed that concurrent administration of crocin and DZN could restore the effects of subchronic DZN administration on SBP and HR in rats.
authors: School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
authors: Marjan
authors: M
authors: Razavi
authors: Hossein
authors: H
authors: Hosseinzadeh
authors: Khalil
authors: K
authors: Abnous
authors: Vahideh Sadat
authors: VS
authors: Motamedshariaty
authors: Mohsen
authors: M
authors: Imenshahidi
keywords: Crocin; Crocus sativus; Diazinon; Heart rate; Malondealdehyde; Systolic blood pressure
references: J Biomed Biotechnol. 2010;2010:829190
references: 20445738
references: J Pharm Pharm Sci. 2005 Aug 22;8(3):387-93
references: 16401388
references: Toxicol Mech Methods. 2007;17(2):109-15
references: 20020979
references: J Vet Med Sci. 1993 Jun;55(3):383-5
references: 8357910
references: Toxicology. 1982;25(2-3):79-97
references: 6760465
references: Probl Khig. 1984;9:59-65
references: 6483829
references: Toxicology. 2002 Mar 5;172(1):13-34
references: 11844612
references: J Pharm Pharm Sci. 2005 Aug 22;8(3):394-9
references: 16401389
references: Toxicol Lett. 2005 Jan 15;155(1):59-64
references: 15585360
references: Phytother Res. 2005 Nov;19(11):997-1000
references: 16317646
references: Phytomedicine. 2007 Sep;14(9):621-7
references: 17291733
references: Pak J Biol Sci. 2010 Jul 15;13(14):691-8
references: 21848061
references: Ecotoxicol Environ Saf. 2007 Mar;66(3):447-50
references: 16797706
references: Cardiovasc Toxicol. 2010 Mar;10(1):66-71
references: 20119744
references: Pharmazie. 2006 Apr;61(4):348-52
references: 16649553
references: Res Vet Sci. 2007 Jun;82(3):405-8
references: 17064743
references: Phytother Res. 2000 Mar;14(2):73-88
references: 10685102
references: Phytomedicine. 2008 Dec;15(12):1038-45
references: 18771905
references: Phytother Res. 2000 May;14(3):149-52
references: 10815004
references: Arkh Patol. 1985;47(1):44-51
references: 3977625
references: Toxicology. 2005 Aug 1;211(3):197-206
references: 15925023
references: C R Biol. 2008 Sep;331(9):655-62
references: 18722984
references: Phytomedicine. 2010 Mar;17(3-4):227-32
references: 19747807
references: Phytother Res. 2010 Jul;24(7):990-4
references: 20013822
references: Am J Physiol Regul Integr Comp Physiol. 2002 Jun;282(6):R1565-82
references: 12010737
references: Food Chem Toxicol. 2010 Oct;48(10):2803-8
references: 20637253
references: Phytomedicine. 2011 Apr 15;18(6):499-504
references: 21036580
references: Phytother Res. 2008 Mar;22(3):330-4
references: 18058985