title: Mechanical properties evolution of a PLGA-PLCL composite scaffold for ligament tissue engineering under static and cyclic traction-torsion in vitro culture conditions.
abstract: This study aims to investigate the in vitro degradation of a poly(L-lactic-co-glycolic acid)-poly(L-lactic-co-ϵ-caprolactone) (PLGA-PLCL) composite scaffold's mechanical properties under static culture condition and 2 h period per day of traction-torsion cyclic culture conditions of simultaneous 10% uniaxial strain and 90° of torsion cycles at 0.33 Hz. Scaffolds were cultured in static conditions, during 28 days, with or without cell seeded or under dynamic conditions during 14 days in a bioreactor. Scaffolds' biocompatibility and proliferation were investigated with Alamar Blue tests and cell nuclei staining. Scaffolds' mechanical properties were tested during degradation by uniaxial traction test. The PLGA-PLCL composite scaffold showed a good cytocompatibility and a high degree of colonization in static conditions. Mechanical tests showed a competition between two process of degradation which have been associated to hydrolytic and enzymatic degradation for the reinforce yarn in poly(L-lactic-co-glycolic acid) (PLGA). The enzymatic degradation led to a decrease effect on mechanical properties of cell-seeded scaffolds during the 21st days, but the hydrolytic degradation was preponderant at day 28. In conclusion, the structure of this scaffold is adapted to culture in terms of biocompatibility and cell orientation (microfiber) but must be improved by delaying the degradation of it reinforce structure in PLGA.
authors: PPIA , UMR-CNRS 7561, Lorraine Université, Vandoeuvre-Lès-Nancy, France. cyril.kahn@ifsttar.fr
authors: Cyril J F
authors: CJ
authors: Kahn
authors: Kahina
authors: K
authors: Ziani
authors: Ye Min
authors: YM
authors: Zhang
authors: Jian
authors: J
authors: Liu
authors: Nguyen
authors: N
authors: Tran
authors: Jérôme
authors: J
authors: Babin
authors: Natalia
authors: N
authors: de Isla
authors: Jean-Luc
authors: JL
authors: Six
authors: Xiong
authors: X
authors: Wang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D019344:Lactic Acid / Q000737:chemistry*; D008022:Ligaments; D008297:Male; D011091:Polyesters / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D051381:Rats; D017208:Rats, Wistar; D013234:Stem Cells / Q000166:cytology; D013718:Tensile Strength; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D011091:Polyesters; C507868:poly(glycolide-co-lactide-co-caprolactone); D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid