title: Effect of bromochloromethane and fumarate on phylogenetic diversity of the formyltetrahydrofolate synthetase gene in bovine rumen.
abstract: Effect of the methane inhibitor, bromochloromethane (BCM) and dietary substrate, fumarate, on microbial community structure of acetogen bacteria in the bovine rumen was investigated through analysis of the formyltetrahydrofolate synthetase gene (fhs). The fhs sequences obtained from BCM-untreated, BCM-treated, fumarate-untreated and fumarate-treated bovine rumen were categorized into homoacetogens and nonhomoacetogenic bacteria by homoacetogen similarity scores. Phylogenetic tree analysis indicated that most of the fhs sequences categorized into homoacetogens were divided into nine clusters, which were in close agreement with a result shown in a self-organizing map. The diversity of the fhs sequences from the BCM-treated rumen was significantly different from those from BCM-non-treated rumen. Principal component analysis also showed that addition of BCM to the rumen altered the population structure of acetogenic bacteria significantly but the effect of fumarate was comparatively minor. These results indicate that BCM affects diversity of actogens in the bovine rumen, and changes in acetogenic community structure in response to methane inhibitors may be caused by different mechanisms.
authors: National Institute of Livestock and Grassland Science, Tsukuba, Japan.
authors: Makoto
authors: M
authors: Mitsumori
authors: Hiroki
authors: H
authors: Matsui
authors: Kiyoshi
authors: K
authors: Tajima
authors: Takumi
authors: T
authors: Shinkai
authors: Akio
authors: A
authors: Takenaka
authors: Stuart E
authors: SE
authors: Denman
authors: Christopher S
authors: CS
authors: McSweeney
mesh_terms: D045855:Acetobacterium / Q000187:drug effects* / Q000201:enzymology*; D000818:Animals; D058491:Bacterial Load / Q000187:drug effects*; D002417:Cattle / Q000382:microbiology*; D005574:Formate-Tetrahydrofolate Ligase / Q000235:genetics*; D005650:Fumarates / Q000494:pharmacology*; D014644:Genetic Variation / Q000187:drug effects* / Q000235:genetics*; D006846:Hydrocarbons, Halogenated / Q000494:pharmacology*; D010802:Phylogeny; D012417:Rumen / Q000382:microbiology*
chemical_list: D005650:Fumarates; D006846:Hydrocarbons, Halogenated; C006811:bromochloromethane; D005574:Formate-Tetrahydrofolate Ligase
keywords: FTHFS; acetogen; bromochloromethane; fumarate; rumen