title: Short-term microbiological effects of scaling and root planing and essential-oils mouthwash in Chinese adults.
abstract: OBJECTIVE
  To assess the short-term effect of scaling and root planing (SRP) and essential-oils mouthwash on the levels of specific bacteria in Chinese adults.
  
  
  METHODS
  Fifty Chinese adults with chronic periodontitis were randomly assigned to full-mouth SRP or a 7-d essential-oils mouthwash regimen. In addition, 22 periodontally healthy adults used essential-oils mouthwash for 7 d. Clinical examination and plaque/saliva sampling were performed at baseline and on Day 7. Quantitative real-time polymerase chain reaction (PCR) was used to measure Aggregatibacter actinomycetemcomitans (Aa), Fusobacterium nucleatum (Fn), Porphyromonas gingivalis (Pg), Prevotella intermedia (Pi), and total bacterial loads in saliva, supra- and sub-gingival plaque samples.
  
  
  RESULTS
  The detection frequencies of four tested species remained unchanged after either treatment. However, the bacterial loads of Fn, Pg, and Pi were significantly reduced by SRP; the mean reduction of bacterial counts in saliva ranged from 52.2% to 62.5% (p<0.01), in supragingival plaque from 68.2% to 81.0% (p<0.05), and in subgingival plaque from 67.9% to 93.0% (p<0.01). Total bacterial loads were reduced after SRP in supra- and sub-gingival plaque (p<0.05). Essential-oils mouthwash reduced Fn levels in supragingival plaque by a mean of 53.2%, and reduced total bacterial loads in supra- and sub-gingival plaque (p<0.01). In subgingival plaque from periodontal patients, Pg and Pi reductions were high after SRP compared to essential-oils mouthwash (93.0% vs. 37.7% and 87.0% vs. 21.0%, p<0.05). No significant bacterial reduction was observed in periodontally healthy subjects using essential-oils mouthwash.
  
  
  CONCLUSIONS
  SRP and essential-oils mouthwash both have an impact on saliva and gingival plaque flora in Chinese periodontitis patients in 7 d, with greater microbiological improvement by SRP.
authors: Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.
authors: Jia-yan
authors: JY
authors: He
authors: Gang-gang
authors: GG
authors: Qi
authors: Wu-jing
authors: WJ
authors: Huang
authors: Xu-dong
authors: XD
authors: Sun
authors: Yu
authors: Y
authors: Tong
authors: Chun-mei
authors: CM
authors: Peng
authors: Xue-ping
authors: XP
authors: Zhou
authors: Hui
authors: H
authors: Chen
mesh_terms: D000328:Adult; D058491:Bacterial Load / Q000187:drug effects; D002681:China; D055113:Chronic Periodontitis / Q000382:microbiology* / Q000517:prevention & control*; D003773:Dental Plaque / Q000382:microbiology / Q000517:prevention & control; D012534:Dental Scaling / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009067:Mouthwashes / Q000627:therapeutic use*; D009822:Oils, Volatile / Q000627:therapeutic use*; D009910:Oral Hygiene / Q000379:methods*; D012463:Saliva / Q000187:drug effects / Q000382:microbiology*; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: D009067:Mouthwashes; D009822:Oils, Volatile
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