title: Technical communication: stability of propofol in polystyrene-based tissue culture plates.
abstract: Propofol has been reported to have high stability in glass and relatively high stability up to 24 hours in polyvinyl chloride-based medical plastics. Recent publications have observed the effects of propofol on cells and tissues grown in culture. Many cell culture plastics are formulated from polystyrene but we could find little information on the stability of propofol exposed to these products. We observed very little change in the concentration of propofol diluted in cell culture medium over 24 hours when exposed to glass, but substantial loss of the drug when exposed to 96-well polystyrene cell culture plates. This decrease was most rapid in the first hour but continued until 24 hours. The type of plastic used in cell and tissue culture experiments with propofol may influence the results by increasing the apparent dose required to see an effect.
authors: Department of Anesthesia and Perioperative Care, University of California, San Francisco, S255, Box 0542, 513 Parnassus Ave., San Francisco, CA 94143-0542, USA. sallj@anesthesia.ucsf.edu
authors: Jeffrey W
authors: JW
authors: Sall
authors: Jason
authors: J
authors: Leong
mesh_terms: D002626:Chemistry, Pharmaceutical / Q000379:methods*; D004355:Drug Stability; D010969:Plastics / Q000009:adverse effects / Q000737:chemistry*; D011137:Polystyrenes / Q000009:adverse effects / Q000737:chemistry*; D015742:Propofol / Q000378:metabolism*; D013997:Time Factors; D046509:Tissue Culture Techniques / Q000295:instrumentation* / Q000592:standards
chemical_list: D010969:Plastics; D011137:Polystyrenes; D015742:Propofol
references: Anesthesiology. 2000 May;92(5):1408-17
references: 10781288
references: J Neurosci Res. 1996 Sep 01;45(5):631-6
references: 8875327
references: Photochem Photobiol Sci. 2006 Nov;5(11):1059-67
references: 17077903
references: Anesth Analg. 2005 Jan;100(1):215-225
references: 15616081
references: Anesthesiology. 2012 Feb;116(2):352-61
references: 22198221
references: Anesthesiology. 2009 Apr;110(4):826-33
references: 19293697
references: J Pharm Biomed Anal. 1997 Jul;15(11):1633-40
references: 9260658
references: J Neurosurg Anesthesiol. 2008 Oct;20(4):233-40
references: 18812886
references: Anesthesiology. 2005 May;102(5):970-6
references: 15851884
references: Anesth Analg. 2000 Jun;90(6):1450-1
references: 10825339