title: Platelet Activation Test in Unprocessed Blood (Pac-t-UB) to Monitor Platelet Concentrates and Whole Blood of Thrombocytopenic Patients.
abstract: BACKGROUND
  Platelet concentrate transfusion is the standard treatment for hemato-oncology patients to compensate for thrombocytopenia. We have developed a novel platelet activation test in anticoagulated unprocessed blood (pac-t-UB) to determine platelet function in platelet concentrates and in blood of thrombocytopenic patients.
  
  
  METHODS
  We have measured platelet activity in a platelet concentrate and in anticoagulated unprocessed blood of a post-transfusion thrombocytopenic patient.
  
  
  RESULTS
  Our data show time-dependent platelet activation by GPVI agonist (collagen related peptide; CRP), PAR-1 agonist (SFLLRN), P2Y12 agonist (ADP), and thromboxane receptor agonist (U46619) in a platelet concentrate. Furthermore, pac-t-UB showed time-dependent platelet activation in unprocessed blood of a post-transfusion patient with thrombocytopenia. Testing platelet function by different agonists in relation to storage show that 3-day-old platelet concentrates are still reactive to the studied agonists. This reactivity rapidly drops for each agonists during longer storage.
  
  
  CONCLUSIONS
  Pac-t-UB is a novel tool to estimate platelet function by different agonists in platelet concentrates and in unprocessed blood of thrombocytopenic patients. In the near future, we will validate whether pac-t-UB is an adequate test to monitor the quality of platelet concentrates and whether pac-t-UB predicts the bleeding risk of transfused thrombocytopenic patients.
authors: Department of Clinical Chemistry and Hematology, University Medical Center Utrecht, Apeldoorn, The Netherlands.
authors: Mark
authors: M
authors: Roest
authors: Thijs C
authors: TC
authors: van Holten
authors: Ger-Jan
authors: GJ
authors: Fleurke
authors: Jasper A
authors: JA
authors: Remijn
keywords: Flow cytometry; Platelet concentrates; Platelet function; Platelet storage; Platelet transfusion; Platelet, Platelet activation; Platelets; Thrombocytopenia
references: Methods Mol Biol. 2004;272:225-53
references: 15226548
references: Vox Sang. 2011 Jul;101(1):1-9
references: 21668862
references: Transfusion. 2013 Aug;53(8):1780-7
references: 23216254
references: Blood. 2012 Jun 7;119(23):5553-62
references: 22496156
references: J Thromb Haemost. 2012 Jun;10(6):1177-9
references: 22429872