title: A novel hemostatic sealant composed of gelatin, transglutaminase and thrombin effectively controls liver trauma-induced bleeding in dogs.
abstract: OBJECTIVE
  novel hemostatic sealant based on the in situ gel formation of gelatin catalyzed by thrombin and crosslinked by transglutaminase was designed. The aim of this study was to investigate the efficacy of the hemostatic sealant in control of bleeding caused by liver trauma in dogs.
  
  
  METHODS
  Hepatic trauma that mimicked the grade III-IV rupture of liver was made in 20 dogs. The traumatic lesion was topically administered the hemostatic sealant (treatment group, n=10), or a thrombin solution (control group, n=10). The time to achieve hemostasis and the blood loss were measured. Contrast-enhanced ultrasound (CEUS) examination was performed in each animal on d 3, d 7, and d 10 d postoperatively to study the healing of the lesions.
  
  
  RESULTS
  The mean time to achieve hemostasis in the treatment group was significantly shorter than that in the control group (1.20±0.33 vs 6.70±0.64 min, P<0.05). The mean blood loss in the treatment group was significantly less than that in the control group (47.22±8.61 vs 79.29±11.97 mL, P<0.05). In CEUS examination, the traumatic lesions in the treatment group became significantly smaller on d 3, and disappeared on d 7, whereas the lesions in the control group still existed on d 10. Ascites were never found during 10 d follow-up in the treatment group but were observed on d 3 and d 7 in the control group.
  
  
  CONCLUSIONS
  Compared with thrombin, the novel hemostatic sealant shows much better efficacy in hemostasis and may promote wound healing in dog liver trauma.
authors: Department of Ultrasound, Chinese People's Liberation Army General Hospital, Beijing, China.
authors: Xia
authors: X
authors: Xie
authors: Jiang-ke
authors: JK
authors: Tian
authors: Fa-qin
authors: FQ
authors: Lv
authors: Rong
authors: R
authors: Wu
authors: Wen-bo
authors: WB
authors: Tang
authors: Yu-kun
authors: YK
authors: Luo
authors: Ya-qin
authors: YQ
authors: Huang
authors: Jie
authors: J
authors: Tang
mesh_terms: D000818:Animals; D016063:Blood Loss, Surgical / Q000517:prevention & control*; D002417:Cattle; D004285:Dogs; D004338:Drug Combinations; D005780:Gelatin / Q000008:administration & dosage*; D006487:Hemostasis / Q000187:drug effects / Q000502:physiology; D006490:Hemostatics / Q000008:administration & dosage*; D008099:Liver / Q000187:drug effects* / Q000293:injuries / Q000601:surgery; D008297:Male; D011897:Random Allocation; D013917:Thrombin / Q000008:administration & dosage*; D011503:Transglutaminases / Q000008:administration & dosage*; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology
chemical_list: D004338:Drug Combinations; D006490:Hemostatics; D005780:Gelatin; D011503:Transglutaminases; D013917:Thrombin
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