title: [Expression of miR-143 in nasopharyngeal carcinoma cell lines and its effect on cell adhesion ability].
abstract: OBJECTIVE
  To detect miR-143 expression in human nasopharyngeal carcinoma (NPC) cell lines and explore the role of miR-143 in regulating the adhesion ability of NPC cells.
  
  
  METHODS
  Fluorescence quantitative RT-PCR was used to detect miR-143 expression levels in 5 NPC cell lines (CEN1, CNE2, HONE1, 5-8F, and 6-10B) and an immortalized human nasopharyngeal epithelial cell line (NP69). The retrovirus plasmid pMSCV-puro-miR-143 was constructed and the packaged retroviruses pMSCV-puro and pMSCV-puro-miR-143 were infected in 5-8F cells to establish a cell line with stable miR-143 overexpression, whose adhesion ability was tested with adhesion assay.
  
  
  RESULTS
  The expression of miR-143 was down- regulated in the NPC cell lines, and the highly metastatic NPC cell line 5-8F had a expression of only 3.0% of the control level, as compared with the level of 63.59% in the tumorigenic but nonmetastatic NPC cell line 6-10B. The transfected 5-8F cells showed a 1080-fold increase of miR-143 expression (P<0.05) with a significantly lowered adhesion ability.
  
  
  CONCLUSIONS
  miR-143 plays a role in regulating the invasiveness and metastasis of NPC, and overexpression of miR-143 causes a significant reduction of the adhesion ability of the highly metastatic NPC cell line 5-8F.
authors: Medical Examination Center, 421 Hospital of PLA, Guangzhou 510318, China.
authors: Wen
authors: W
authors: Zhong
authors: Benfu
authors: B
authors: He
authors: Tianyi
authors: T
authors: Quan
authors: Chengquan
authors: C
authors: Zhu
authors: Silang
authors: S
authors: Zhou
authors: Yongle
authors: Y
authors: Chen
mesh_terms: D002277:Carcinoma; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D002465:Cell Movement; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism* / Q000473:pathology*
chemical_list: C542359:MIRN143 microRNA, human; D035683:MicroRNAs