title: Specific IgG response against Mycobacterium avium paratuberculosis in children and adults with Crohn's disease.
abstract: OBJECTIVE
  Presence of serum antibodies against Mycobacterium avium paratuberculosis (MAP) in Crohn's Disease (CD) as a disease characteristic remains controversial. In the present work, we assessed antibody reactivity of serum and intestinal fluid against four distinct MAP-antigens, including the recently identified MAP-specific lipopentapeptide (L5P).
  
  
  METHODS
  Immunoglobulin concentrations and specificity against 3 non MAP-specific antigens: glycosyl-transferase-d (GSD), purified protein derivative from MAP (Johnin-PPD), heparin binding haemagglutinin (MAP-HBHA) and one MAP-specific antigen: synthetic L5P were determined by ELISA in gut lavage fluids from adult controls or patients with CD, and in sera of children or adult controls or patients with CD, ulcerative colitis or celiac disease.
  
  
  RESULTS
  Total IgA and IgG concentrations were increased in sera of children with CD but were decreased in sera of adults with CD, thereof specificity against MAP antigens was assessed by normalizing immunoglobulin concentrations between samples. In CD patients, IgG reactivity was increased against the four MAP antigens, including L5P in gut lavage fluids but it was only increased against L5P in sera. By contrast, anti-L5P IgG were not increased in patients with ulcerative colitis or celiac disease.
  
  
  CONCLUSIONS
  A significant increase in anti-L5P IgG is observed in sera of children and adults with CD but not in patients with other intestinal inflammatory diseases. Anti-L5P antibodies may serve as serological marker for CD.
authors: Institut National de la Santé et de la Recherche Médicale (INSERM), UMR989, Paris, France.
authors: Julien
authors: J
authors: Verdier
authors: Louis
authors: L
authors: Deroche
authors: Matthieu
authors: M
authors: Allez
authors: Caroline
authors: C
authors: Loy
authors: Franck
authors: F
authors: Biet
authors: Christelle C
authors: CC
authors: Bodier
authors: Sylvie
authors: S
authors: Bay
authors: Christelle
authors: C
authors: Ganneau
authors: Tamara
authors: T
authors: Matysiak-Budnik
authors: Jean Marc
authors: JM
authors: Reyrat
authors: Martine
authors: M
authors: Heyman
authors: Nadine
authors: N
authors: Cerf-Bensussan
authors: Frank M
authors: FM
authors: Ruemmele
authors: Sandrine
authors: S
authors: Ménard
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D003424:Crohn Disease / Q000097:blood* / Q000276:immunology / Q000382:microbiology*; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology*; D007422:Intestines / Q000276:immunology / Q000382:microbiology; D055666:Lipopeptides / Q000276:immunology; D008297:Male; D008875:Middle Aged; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology* / Q000502:physiology*; D055815:Young Adult
chemical_list: D000942:Antigens, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D055666:Lipopeptides
references: Curr Opin Gastroenterol. 2010 Jul;26(4):332-6
references: 20571385
references: Science. 2004 Mar 12;303(5664):1662-5
references: 15016999
references: J Biol Chem. 2000 May 12;275(19):14273-80
references: 10799506
references: J Med Microbiol. 1984 Dec;18(3):309-18
references: 6438323
references: Gastroenterology. 2006 Jul;131(1):85-96
references: 16831593
references: Clin Infect Dis. 2002 Dec 1;35(11):1328-35
references: 12439795
references: Nature. 2012 Nov 1;491(7422):119-24
references: 23128233
references: Nat Rev Microbiol. 2010 Aug;8(8):564-77
references: 20622892
references: Infect Immun. 2001 Mar;69(3):1492-8
references: 11179318
references: J Crohns Colitis. 2010 Feb;4(1):7-27
references: 21122488
references: Vet Immunol Immunopathol. 2012 Jul 15;148(1-2):55-68
references: 22486997
references: World J Gastroenterol. 2008 Jun 21;14(23):3650-61
references: 18595132
references: Curr Opin Gastroenterol. 2008 Jan;24(1):17-21
references: 18043227
references: J Biol Chem. 2006 Feb 24;281(8):5209-15
references: 16339155
references: Gastroenterology. 1989 Dec;97(6):1562-84
references: 2684725
references: Inflamm Bowel Dis. 2007 Dec;13(12):1561-6
references: 17636565
references: J Crohns Colitis. 2008 Mar;2(1):24-62
references: 21172195
references: Microbiology (Reading). 1998 Dec;144 ( Pt 12):3413-3423
references: 9884234
references: Clin Microbiol Rev. 1989 Jan;2(1):90-117
references: 2644025
references: Lancet Infect Dis. 2003 Aug;3(8):507-14
references: 12901893
references: Gastroenterology. 2011 May;140(6):1720-28
references: 21530738
references: J Food Prot. 2011 Mar;74(3):480-99
references: 21375889
references: Vaccine. 2008 Jan 10;26(2):257-68
references: 18068277
references: J Immunol Methods. 1988 May 25;110(1):85-91
references: 3373004
references: Clin Infect Dis. 2009 Aug 15;49(4):529-35
references: 19591595
references: PLoS Pathog. 2005 Nov;1(3):279-85
references: 16322770
references: Arq Gastroenterol. 2010 Jul-Sep;47(3):242-5
references: 21140083
references: Clin Microbiol Rev. 2007 Apr;20(2):205-29
references: 17428883
references: Gastroenterology. 2006 Jun;130(7):2050-9
references: 16762628
references: Nat Rev Immunol. 2008 Jun;8(6):458-66
references: 18500230
references: J Med Chem. 2008 Jan 10;51(1):167-72
references: 18072728
references: Ann Rheum Dis. 1992 Dec;51(12):1296-300
references: 1485810
references: Annu Rev Immunol. 2010;28:573-621
references: 20192811
references: Biopolymers. 2000;55(4):319-33
references: 11169923
references: Int J Syst Evol Microbiol. 2002 Sep;52(Pt 5):1505-1518
references: 12361252
references: J Med Microbiol. 2006 Feb;55(Pt 2):189-199
references: 16434712
references: J Comp Pathol. 1986 Nov;96(6):659-69
references: 3819045
references: Gastroenterology. 1998 Oct;115(4):822-9
references: 9753483
references: J Exp Med. 1996 Sep 1;184(3):993-1001
references: 9064359
references: Int J Syst Bacteriol. 1990 Jul;40(3):254-60
references: 2397193
references: Dig Dis Sci. 2011 Jun;56(6):1794-800
references: 21221802
references: Lancet Infect Dis. 2007 Sep;7(9):607-13
references: 17714674
references: Vet Microbiol. 2009 Mar 2;134(3-4):346-52
references: 18926647
references: Gut. 1990 Dec;31(12):1341-4
references: 2265775
references: Vaccine. 2006 Jan 16;24(3):236-43
references: 16337720
references: Can J Comp Med Vet Sci. 1958 May;22(5):157-60
references: 17649047
references: J Leukoc Biol. 2007 Oct;82(4):1011-8
references: 17652449
references: J Immunol Methods. 1995 Feb 13;179(1):31-5
references: 7868922
references: Annu Rev Immunol. 2005;23:367-86
references: 15771575
references: J Clin Microbiol. 2007 Mar;45(3):968-71
references: 17251406
references: J Pediatr Gastroenterol Nutr. 2005 Jul;41(1):1-7
references: 15990620
references: Infect Immun. 1999 Dec;67(12):6510-7
references: 10569769
references: Arch Dis Child. 1990 Aug;65(8):909-11
references: 2205160
references: Clin Microbiol Rev. 2004 Jan;17(1):98-106
references: 14726457