title: Isolation and characterization of ten polymorphic microsatellite loci in Ixodes arboricola, and cross-amplification in three other Ixodes species.
abstract: We characterized ten polymorphic microsatellite loci from the tree-hole tick, Ixodes arboricola. Loci were screened in 11-18 individuals from three Belgian populations and five to ten alleles were found at each locus. Seven loci did not show deviations from Hardy-Weinberg equilibrium conditions and there were no indications for null alleles at these loci. The three other loci showed significant heterozygote deficiencies in at least one population, and a high potential for the occurrence of null alleles. We observed no effect of potential host DNA on the scoring of the microsatellites. Cross-amplification of the microsatellites was tested in eight specimens of three congeneric species: I. ricinus, I. hexagonus and I. frontalis. Depending on the species, six or seven of the loci were amplified in ≥ 4 of the 8 specimens and were polymorphic in each of these species (except for Ixaf 11 in I. frontalis and I. ricinus). These loci thus provide a tool for population genetic analysis of I. arboricola. The suitability of these markers needs to be further investigated in its congeners.
authors: Evolutionary Ecology Group, University of Antwerp, Groenenborgerlaan 171, 2020, Antwerp, Belgium.
authors: N
authors: N
authors: Van Houtte
authors: A R
authors: AR
authors: Van Oosten
authors: K
authors: K
authors: Jordaens
authors: E
authors: E
authors: Matthysen
authors: T
authors: T
authors: Backeljau
authors: D J A
authors: DJ
authors: Heylen
mesh_terms: D000483:Alleles; D000818:Animals; D004247:DNA / Q000235:genetics; D018884:Ixodes / Q000145:classification* / Q000235:genetics*; D018895:Microsatellite Repeats; D011110:Polymorphism, Genetic; D013045:Species Specificity
chemical_list: D004247:DNA
references: Infect Genet Evol. 2011 Dec;11(8):2043-8
references: 21983685
references: Mol Ecol Resour. 2008 Jan;8(1):103-6
references: 21585727
references: Mol Ecol. 2009 Nov;18(21):4450-62
references: 19793353
references: J Med Entomol. 1965 Apr;2:37-40
references: 14302109
references: Evolution. 1989 Jan;43(1):223-225
references: 28568501
references: J Med Entomol. 2009 May;46(3):471-4
references: 19496415
references: Proc Biol Sci. 2005 Nov 22;272(1579):2389-95
references: 16243689
references: Vet Parasitol. 2008 Aug 17;155(3-4):328-32
references: 18585865
references: Parasitology. 2012 Feb;139(2):264-70
references: 22067275
references: Parasitology. 2010 Apr;137(4):661-7
references: 20025822
references: Parasitology. 2011 Jul;138(8):1011-21
references: 21733259
references: Evol Appl. 2010 Jul;3(4):391-401
references: 25567933
references: Environ Microbiol. 2013 Feb;15(2):663-73
references: 23279105
references: Heredity (Edinb). 2011 Feb;106(2):253-60
references: 20606689
references: Parasitology. 2004;129 Suppl:S191-220
references: 15938512
references: Methods Enzymol. 2005;395:202-22
references: 15865969
references: Annu Rev Entomol. 1996;41:141-61
references: 8546444
references: Mol Ecol. 2009 Sep;18(17):3581-92
references: 19674309
references: Exp Appl Acarol. 2007;42(4):225-38
references: 17674128
references: Parasite. 2008 Sep;15(3):439-43
references: 18814719
references: Infect Genet Evol. 2007 Mar;7(2):308-32
references: 16949350
references: Infect Genet Evol. 2010 Oct;10(7):989-97
references: 20601167
references: Mol Ecol. 2005 Jul;14(8):2247-57
references: 15969711
references: Evolution. 2003 Feb;57(2):288-96
references: 12683525
references: Mol Ecol Resour. 2008 Jan;8(1):92-4
references: 21585724
references: Vector Borne Zoonotic Dis. 2011 Sep;11(9):1235-41
references: 21612531
references: Insect Mol Biol. 2007 Apr;16(2):175-85
references: 17298557
references: Infect Genet Evol. 2007 Mar;7(2):298-304
references: 17215171