title: [Liver monoamine oxidase activity of the lamprey Lampetra fluviatilis. the substrate-inhibitory specificity].
abstract: Based on data of substrate-inhibitory analysis with use of specific inhibitors--deprenyl, chlorgi-lin--and specific substrates--serotonin, noradrenalin, benzylamine, beta-phenylethylamine, and N-methylhistamine--a suggestion is put forward about the possible existence of one molecular form of monoamine oxidase (MAO) in liver of mature individuals of the European lamprey Lampetra fluviatilis. There are determined kinetic parameters of monoamine oxidase deamination of eight substrates, which indicates the large spectrum of substrate specificity of the lamprey liver MAO. The studied enzyme does not deaminate histamine and putrescine and is not sensitive to 10(-2) M semicarbaside. Results of study of the substrate-inhibitor specificity allow us to suggest some resemblance of catalytic properties of the lamprey liver MAO and the mammalian form A MAO. The revealed low activity of the enzyme at deamination of all used substrates seems to be connected with low detoxational functional of the lamprey liver.
authors: O V
authors: OV
authors: Iagodina
authors: I N
authors: IN
authors: Basova
mesh_terms: D000818:Animals; D001596:Benzylamines / Q000494:pharmacology; D003010:Clorgyline / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D007798:Lampreys / Q000097:blood / Q000378:metabolism; D008761:Methylhistamines / Q000378:metabolism; D008930:Mitochondria, Liver / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008995:Monoamine Oxidase / Q000378:metabolism*; D008996:Monoamine Oxidase Inhibitors / Q000494:pharmacology; D010627:Phenethylamines / Q000378:metabolism; D012642:Selegiline / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D001596:Benzylamines; D008761:Methylhistamines; D008996:Monoamine Oxidase Inhibitors; D010627:Phenethylamines; D012642:Selegiline; C029261:phenethylamine; D012701:Serotonin; C030796:benzylamine; D008995:Monoamine Oxidase; D003010:Clorgyline; C048140:N-methylhistamine