title: The stability of BMP loaded polyelectrolyte multilayer coatings on titanium.
abstract: Immobilization of bone morphogenetic proteins (BMP) onto material surfaces is a promising, but still challenging, strategy for achieving dependable and consistent osseointegration of long-term metal implants. In the present study, we have developed an osteoinductive coating of a porous titanium implant using biomimetic polyelectrolyte multilayer (PEM) films loaded with BMP-2. The amount of BMP-2 loaded in these films was tuned - over a large range - depending on the cross-linking extent of the film and of the BMP-2 initial concentration. The air-dried PEM films were stable for at least one year of storage at 4 °C. In addition, they resisted exposure to γ-irradiation at clinically approved doses. The preservation of the growth factor bioactivity upon long-term storage and sterilization were evaluated both in vitro (using C2C12 cells) and in vivo (in a rat ectopic model) for the perspective of industrial and clinical development. BMP-2 loaded in dried PEM films exhibited shelf-life stability over one year. However, their bioactivity in vitro decreased from 50 to 80% after irradiation depending on the γ-irradiation dose. Remarkably, the in vivo studies showed that the osteoinductive potential of BMP-2 contained in PEM-coated Ti implants was fully preserved after air-drying of the implants and sterilization at 25 kGy. Film drying or irradiation did not affect the amount of new bone tissue formation. This "off-the-shelf" novel technology of functionalized implants opens promising applications in prosthetic and tissue engineering fields.
authors: UMR 5628 (LMGP), Grenoble Institute of Technology and CNRS, 3 Parvis Louis Néel, F-38016 Grenoble Cedex, France.
authors: Raphael
authors: R
authors: Guillot
authors: Flora
authors: F
authors: Gilde
authors: Pierre
authors: P
authors: Becquart
authors: Frédéric
authors: F
authors: Sailhan
authors: Aurélien
authors: A
authors: Lapeyrere
authors: Delphine
authors: D
authors: Logeart-Avramoglou
authors: Catherine
authors: C
authors: Picart
mesh_terms: D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects / Q000528:radiation effects; D002460:Cell Line; D020099:Coated Materials, Biocompatible / Q000494:pharmacology*; D004573:Electrolytes / Q000737:chemistry*; D005720:Gamma Rays; D006820:Hyaluronic Acid / Q000031:analogs & derivatives* / Q000737:chemistry; D051379:Mice; D010012:Osteogenesis / Q000187:drug effects / Q000528:radiation effects; D011107:Polylysine / Q000031:analogs & derivatives* / Q000737:chemistry; D016062:Porosity; D051381:Rats; D013242:Sterilization; D054457:Tissue Scaffolds / Q000737:chemistry; D014025:Titanium / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D019485:Bone Morphogenetic Proteins; D020099:Coated Materials, Biocompatible; D004573:Electrolytes; D011107:Polylysine; D006820:Hyaluronic Acid; D014025:Titanium
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