title: Lung inflammation induces IL-1β expression in hypoglossal neurons in rat brainstem.
abstract: Perinatal inflammation is associated with respiratory morbidity. Immune modulation of brainstem respiratory control centers may provide a link for this pathobiology. We exposed 11-day old rats to intratracheal lipopolysaccharide (LPS, 0.5 μg/g) to test the hypothesis that intrapulmonary inflammation increases expression of the proinflammatory cytokine IL-1β within respiratory-related brainstem regions. Intratracheal LPS resulted in a 32% increase in IL-1β protein expression in the medulla oblongata. In situ hybridization showed increased intensity of IL-1β mRNA but no change in neuronal numbers. Co-localization experiments showed that hypoglossal neurons express IL-1β mRNA and immunostaining showed a 43% increase in IL-1β protein-expressing cells after LPS exposure. LPS treatment also significantly increased microglial cell numbers though they did not express IL-1β mRNA. LPS-induced brainstem expression of neuronal IL-1β mRNA and protein may have implications for our understanding of the vulnerability of neonatal respiratory control in response to a peripheral proinflammatory stimulus.
authors: Department of Pediatrics, Division of Neonatology, Rainbow Babies & Children's Hospital, Cleveland, OH 44106, USA.
authors: Anjum
authors: A
authors: Jafri
authors: Abdelmadjid
authors: A
authors: Belkadi
authors: Syed I A
authors: SI
authors: Zaidi
authors: Paulina
authors: P
authors: Getsy
authors: Christopher G
authors: CG
authors: Wilson
authors: Richard J
authors: RJ
authors: Martin
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001933:Brain Stem / Q000187:drug effects / Q000378:metabolism*; D005786:Gene Expression Regulation; D007002:Hypoglossal Nerve / Q000187:drug effects / Q000378:metabolism*; D053583:Interleukin-1beta / Q000096:biosynthesis*; D008070:Lipopolysaccharides / Q000633:toxicity; D011014:Pneumonia / Q000139:chemically induced / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D053583:Interleukin-1beta; D008070:Lipopolysaccharides
references: Respir Physiol Neurobiol. 2011 Sep 30;178(3):414-21
references: 21609789
references: J Histochem Cytochem. 2006 Nov;54(11):1303-13
references: 16899759
references: Proc Natl Acad Sci U S A. 2007 Jun 5;104(23):9894-9
references: 17535900
references: Neurotoxicology. 2012 Jun;33(3):347-60
references: 22330755
references: Curr Opin Neurol. 2005 Apr;18(2):117-23
references: 15791140
references: Acta Paediatr. 2012 May;101(5):466-71
references: 22176020
references: J Neuroinflammation. 2010 Nov 22;7:82
references: 21092194
references: Exp Neurol. 2001 Mar;168(1):23-31
references: 11170718
references: Respir Physiol Neurobiol. 2011 Sep 30;178(3):458-64
references: 21397055
references: Pediatr Res. 2007 Aug;62(2):134-8
references: 17597656
references: Brain Res Mol Brain Res. 1994 Nov;27(1):27-36
references: 7877451
references: J Neuroimmunol. 2009 Jan 3;206(1-2):44-51
references: 19081643
references: Neuron. 2009 Oct 15;64(1):25-7
references: 19840544
references: Glia. 2001 Mar 1;33(3):256-66
references: 11241743
references: J Neuroimmunol. 2001 Aug 30;118(2):203-11
references: 11498255
references: Pediatr Res. 2012 Jun;71(6):645-52
references: 22337231
references: J Neuroinflammation. 2009 Mar 11;6:9
references: 19284588
references: J Clin Invest. 1997 Dec 15;100(12):2941-7
references: 9399938
references: J Neuroinflammation. 2013 Apr 05;10:45
references: 23561827
references: Neuron. 2009 Oct 15;64(1):61-78
references: 19840550
references: Pediatr Res. 2003 Sep;54(3):326-31
references: 12761362
references: Mol Cell Neurosci. 2012 Mar;49(3):311-21
references: 22270046
references: Exp Mol Med. 2010 Dec 31;42(12):823-32
references: 20962566
references: Crit Care Nurse. 2001 Dec;21(6):35-47
references: 11767763
references: Annu Rev Physiol. 1994;56:93-116
references: 7912060
references: Pediatr Pulmonol. 2008 Oct;43(10):937-44
references: 18780339
references: Brain Res. 2006 Feb 3;1071(1):42-53
references: 16413509