title: Changes of calcium binding proteins, c-Fos and COX in hippocampal formation and cerebellum of Niemann-Pick, type C mouse.
abstract: Niemann-Pick disease, type C (NPC) is an intractable disease that is accompanied by ataxia, dystonia, neurodegeneration, and dementia due to an NPC gene defect. Disruption of calcium homeostasis in neurons is important in patients with NPC. Thus, we used immunohistochemistry to assess the expression levels of calcium binding proteins (calbindin D28K, parvalbumin, and calretinin), c-Fos and cyclooxygenase-1,2 (COX-1,2) in the hippocampal formation and cerebellum of 4 and 8 week old NPC+/+, NPC+/-, and NPC-/- mice. General expression of these proteins decreased in the hippocampus and cerebellum of NPC-/- compared to that in both young and adult NPC+/+ or NPC+/- mice. Parvalbumin, COX-1,2 or c-Fos-immunoreactive neurons were widely detected in the CA1, CA3, and DG of the hippocampus, but the immunoreactivities were decreased sharply in all areas of hippocampus of NPC-/- compared to NPC+/+ and NPC+/- mice. Taken together, reduction of these proteins may be one of the strong phenotypes related to the neuronal degeneration in NPC-/- mice.
authors: Center for Regenerative Medicine, Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 406-840, South Korea; Department of Anatomy and Cell Biology, Gachon University Graduate School of Medicine, Incheon 406-799, South Korea.
authors: Kyunghee
authors: K
authors: Byun
authors: Daesik
authors: D
authors: Kim
authors: Enkhjaigal
authors: E
authors: Bayarsaikhan
authors: Jeehyun
authors: J
authors: Oh
authors: Jisun
authors: J
authors: Kim
authors: Grace
authors: G
authors: Kwak
authors: Goo-Bo
authors: GB
authors: Jeong
authors: Seung-Mook
authors: SM
authors: Jo
authors: Bonghee
authors: B
authors: Lee
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D002531:Cerebellum / Q000378:metabolism* / Q000473:pathology; D051545:Cyclooxygenase 1 / Q000378:metabolism*; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D005260:Female; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D008822:Mice, Transgenic; D052556:Niemann-Pick Disease, Type C / Q000378:metabolism* / Q000473:pathology; D016760:Proto-Oncogene Proteins c-fos / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000378:metabolism*
chemical_list: D002135:Calcium-Binding Proteins; D008565:Membrane Proteins; D016760:Proto-Oncogene Proteins c-fos; C077623:Ptgs2 protein, mouse; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; C496512:Ptgs1 protein, mouse
keywords: COX-1/COX-2; Calcium binding proteins; Cerebellum; DG; GABA; HP; Hippocampus; NADP; NPC; NPC knock-out mice; Niemann–Pick disease, type C; PBS; PCR; cyclooxygenase-1/2; dentate gyrus; gamma aminobutyric acid; hippocampus; nicotinic acid adenine dinucleotide phosphate; phosphate-buffered saline; polymerase chain reaction