title: Cloning and characterization of a novel human BRMS1 transcript variant in hepatocellular carcinoma cells.
abstract: Breast cancer metastasis suppressor 1 (BRMS1) is able to suppress tumor metastasis without affecting primary tumor growth in various cancers. Here, we report a novel transcript variant of human BRMS1, termed BRMS1.vh. BRMS1.vh is identical to the major BRMS1 variant (BRMS1.v1) except for missing base pairs 683-775, encoding a 215-amino acid protein lacking a functional nuclear localization sequence. Expression of BRMS1.vh in hepatocellular carcinoma (HCC) cells suppressed NF-κB signaling pathway, sensitized cells to apoptotic stimuli, leading to suppressed tumor growth. Taken together, our results suggest a potential role for BRMS1.vh in regulating cell apoptosis and tumor growth in HCC.
authors: State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200433, PR China.
authors: Jun
authors: J
authors: Wu
authors: Yingming
authors: Y
authors: Wang
authors: Xiaojing
authors: X
authors: Qiao
authors: Hexige
authors: H
authors: Saiyin
authors: Shenan
authors: S
authors: Zhao
authors: Shouyi
authors: S
authors: Qiao
authors: Yanhua
authors: Y
authors: Wu
mesh_terms: D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D017209:Apoptosis; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003001:Cloning, Molecular; D005260:Female; D017930:Genes, Reporter; D014644:Genetic Variation; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000378:metabolism; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D012097:Repressor Proteins; D015398:Signal Transduction; D013997:Time Factors; D014158:Transcription, Genetic; D014162:Transfection; D047368:Tumor Burden
chemical_list: C410024:BRMS1 protein, human; D016328:NF-kappa B; D009363:Neoplasm Proteins; D012097:Repressor Proteins
keywords: Apoptosis; Breast cancer metastasis suppressor 1 (BRMS1); Hepatocellular carcinoma (HCC); Transcript variant