title: [Influence of ethylbenzene on oxidative damage and apoptosis in rat renal epithelial cells NRK-52e].
abstract: OBJECTIVE
  To study the oxidative damage and apoptosis of renal tubular epithelial cells (NRK-52e cell line) induced by ethylbenzene.
  
  
  METHODS
  NRK-52e cells were exposed to 30, 60, 90, 120 μmol/L ethylbenzene for 24 hours. Cell viability were measured using MTT, the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT), the contents of malondialdehyde (MDA) and glutathione (GSH) were detected respectively. PI fluorescent staining assay was applied to detect percentage of apoptosis in ethylbenzene-treated groups.
  
  
  RESULTS
  Compared with control group, cell outline became clear, cell diopter increased, cell became smaller and shrinkage, some cells broke in 60 μmol/L ethylbenzene-treated group. Plenty of cells died, suspension cells increased significantly in 90 μmol/L ethylbenzene-treated group. Compared with control group, cell viability the activities of SOD and CAT and the content of GSH were significantly decreased in 60 and 90 μmol/L ethylbenzene-treated groups (P<0.05). The MDA content were remarkably elevated in 90 μmol/L ethylbenzene-treated groups (P<0.05).
  
  
  CONCLUSIONS
  Ethylbenzene can induce oxidative stress and apoptosis in NRK-52e cells (P<0.05).
authors: Tianjin University of Traditional Chinese Medicine 300193, China.
authors: Xiao-ting
authors: XT
authors: Liu
authors: De-yi
authors: DY
authors: Yang
authors: Yan-rang
authors: YR
authors: Wang
authors: Qian
authors: Q
authors: Wang
authors: Dan
authors: D
authors: Kuang
authors: Ming
authors: M
authors: Zhang
authors: Li-jie
authors: LJ
authors: Qiao
authors: Jian-guo
authors: JG
authors: Li
authors: Xue-ying
authors: XY
authors: Yang
authors: Shu-lan
authors: SL
authors: Zhao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001555:Benzene Derivatives / Q000633:toxicity*; D002460:Cell Line; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism; D007684:Kidney Tubules / Q000166:cytology; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D001555:Benzene Derivatives; D017382:Reactive Oxygen Species; C004912:ethylbenzene