title: Streptomyces kebangsaanensis sp. nov., an endophytic actinomycete isolated from an ethnomedicinal plant, which produces phenazine-1-carboxylic acid.
abstract: A spore-forming streptomycete designated strain SUK12(T) was isolated from a Malaysian ethnomedicinal plant. Its taxonomic position, established using a polyphasic approach, indicates that it is a novel species of the genus Streptomyces. Morphological and chemical characteristics of the strain were consistent with those of members of the genus Streptomyces. Analysis of the almost complete 16S rRNA gene sequence placed strain SUK12(T) in the genus Streptomyces where it formed a distinct phyletic line with recognized species of this genus. The strain exhibited highest sequence similarity to Streptomyces corchorusii DSM 40340(T) (98.2 %) followed by Streptomyces chrestomyceticus NRRL B-3310(T) (98.1 %). The G+C content of the genomic DNA was 74 mol%. Chemotaxonomic data [MK-9(H8) as the major menaquinone; LL-diaminopimelic acid as a component of cell-wall peptidoglycan; C12 : 0, C14 : 0, C15 : 0 and C17 : 1 as the major fatty acids; phospholipid type II] supported the affiliation of strain SUK12(T) to the genus Streptomyces. The results of the phylogenetic analysis and phenotypic data derived from this and previous studies allowed the genotypic and phenotypic differentiation of strain SUK12(T) from the related species of the genus Streptomyces. The DNA-DNA relatedness value between strain SUK12(T) and S. corchorusii DSM 40340(T) is 18.85±4.55 %. Strain SUK12(T) produces phenazine-1-carboxylic acid, known as tubermycin B, an antibacterial agent. It is proposed, therefore, that strain SUK12(T) ( = DSM 42048(T) = NRRL B-24860(T)) be classified in the genus Streptomyces as the type strain of Streptomyces kebangsaanensis sp. nov.
authors: Centre of Studies for PreClinical Science, Faculty of Dentistry, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia.
authors: Nurul' Izzah Mohd
authors: NIM
authors: Sarmin
authors: Division of Microbiology, Institute of Biological Sciences, Faculty of Science, University of Malaya 50603 Kuala Lumpur, Malaysia.
authors: Geok Yuan Annie
authors: GYA
authors: Tan
authors: Department of Medical Biotechnology, School of Medicine, Flinders University, Bedford Park, South Australia 5042, Australia.
authors: Christopher M M
authors: CMM
authors: Franco
authors: Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, The John Arbuthnott Building, 161 Cathedral Street, Glasgow G4 0RE, Scotland.
authors: RuAngelie
authors: R
authors: Edrada-Ebel
authors: School of Science Chemistry and Food Technology, Faculty of Science & Technology, Universiti Kebangsaan Malaysia 43600 Bangi, Selangor, Malaysia.
authors: Jalifah
authors: J
authors: Latip
authors: Programme of Biomedical Science, School of Diagnostic and Applied Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia 50300 Kuala Lumpur, Malaysia.
authors: Noraziah Mohamad
authors: NM
authors: Zin
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D008296:Malaysia; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000032:analysis; D010619:Phenazines / Q000378:metabolism; D010802:Phylogeny; D010946:Plants, Medicinal / Q000382:microbiology*; D031822:Portulaca / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013302:Streptomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010619:Phenazines; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C037165:1-phenazinecarboxylic acid; C073355:menaquinone 9; D003960:Diaminopimelic Acid