title: Postnatal regulation of X,K-ATPases in rat skin and conserved lateroapical polarization of Na,K-ATPase in vertebrate epidermis.
abstract: Development of epidermis creates stratified epithelium with different sets of ion-transporting enzymes in its layers. We have characterized expression of Na,K- and H,K-ATPase α and β subunits and FXYD isoforms in rat skin. Maturation of rat skin from newborn to adult is associated with an increase in FXYD4 and a decrease of Na,K-ATPase α1-isoform, ATP1B4 and FXYD6 transcripts. Na,K-ATPase of rat epidermis is represented predominantly by α1 and β3 isoforms. Keratinization is associated with the loss of the Na,K-ATPase α-subunit and an enrichment of αng. Na,K-ATPase α1 is abundant in the innermost layer, stratum basale, where it is lacking in basal membranes, thus indicating lateroapical polarization of Na,K-ATPase. Immunocytochemical detection of Na,K-ATPase in Xenopus laevis skin shows that cellular and subcellular localization of the enzyme has a pattern highly similar to that of mammals: basolateral in glandular epithelium and lateroapical in epidermis.
authors: Nikolay B
authors: NB
authors: Pestov
authors: Tatyana V
authors: TV
authors: Korneenko
authors: Mikhail I
authors: MI
authors: Shakhparonov
authors: Nikolai N
authors: NN
authors: Modyanov
mesh_terms: D000818:Animals; D004817:Epidermis / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D017506:H(+)-K(+)-Exchanging ATPase / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D007473:Ion Channels; D007527:Isoenzymes / Q000378:metabolism; D015603:Keratinocytes / Q000166:cytology; D007633:Keratins / Q000378:metabolism; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D012867:Skin / Q000201:enzymology / Q000254:growth & development*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*; D013045:Species Specificity; D014157:Transcription Factors; D014982:Xenopus laevis
chemical_list: C585649:ATP1B4 protein, human; D007473:Ion Channels; D007527:Isoenzymes; D014157:Transcription Factors; C425298:phosphohippolin; D007633:Keratins; C088641:ATP12A protein, human; D017506:H(+)-K(+)-Exchanging ATPase; D000254:Sodium-Potassium-Exchanging ATPase