title: The N-terminal region of two-pore channel 1 regulates trafficking and activation by NAADP.
abstract: TPCs (two-pore channels) are NAADP (nicotinic acid-adenine dinucleotide phosphate)-sensitive Ca2+-permeable ion channels expressed on acidic organelles. In the present study we show that deletion of the N-terminal region redirects TPC1 to the ER (endoplasmic reticulum). The introduction of fluorophores at the N-terminus of TPC1 does not affect its subcellular location, but does reversibly abolish NAADP sensitivity. Our results reveal a dual role for the N-terminus in localization and function of TPC1.
authors: Department of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK.
authors: Dev
authors: D
authors: Churamani
authors: Robert
authors: R
authors: Hooper
authors: Taufiq
authors: T
authors: Rahman
authors: Eugen
authors: E
authors: Brailoiu
authors: Sandip
authors: S
authors: Patel
mesh_terms: D015220:Calcium Channels / Q000502:physiology*; D020013:Calcium Signaling / Q000502:physiology*; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D006801:Humans; D009249:NADP / Q000031:analogs & derivatives* / Q000502:physiology; D010446:Peptide Fragments / Q000494:pharmacology; D000097903:Two-Pore Channels
chemical_list: D015220:Calcium Channels; D009249:NADP; D010446:Peptide Fragments; C407357:TPCN1 protein, human; D000097903:Two-Pore Channels; C024376:NAADP