title: Intestinal protein expression profile identifies inflammatory bowel disease and predicts relapse.
abstract: To date, most studies have applied individual factors as indicators of disease classification and prognosis. The aim of this study is to determine whether clustering analysis of protein expression profiles in intestinal epithelia improves classification and prognosis in patients with inflammatory bowel disease (IBD). One hundred and twenty Crohn's disease (CD) patients, 117 ulcerative colitis (UC) patients and 120 cases of nonspecific colitis provided intestinal biopsy samples for tissue microarray (TMA). Both unsupervised and supervised analyses were used for evaluation of clustering and association with relapse. There was a significant concordance between cluster groups based on immunostaining data of TMA and clinical classification in distinguishing IBD from nonspecific colitis (kappa= 0.498, p<0.001). CD27, CD70, CD40, TRAF3, TRAF4 and TRAF2 presented similar immunostaining features, which were different from clusters of CD154, CD80 and TRAF5. Moreover, higher expression of TRAF2 was a predictor of relapse in patients with UC (p=0.006).Thus, protein expression profiles can distinguish IBD and nonspecific colitis, and combination analysis protein expression profiles show that TRAF2 can predict relapse of UC.
authors: Division of Gastroenterology and Hepatology, Shanghai Jiao-Tong University School of Medicine, Renji Hospital, Shanghai Institute of Digestive Disease, Shanghai, 200127, China.
authors: Jun
authors: J
authors: Shen
authors: Yuqi
authors: Y
authors: Qiao
authors: Zhihua
authors: Z
authors: Ran
authors: Tianrong
authors: T
authors: Wang
authors: Jiangtao
authors: J
authors: Xu
authors: Jinsun
authors: J
authors: Feng
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000032:analysis*; D016000:Cluster Analysis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D015212:Inflammatory Bowel Diseases / Q000145:classification* / Q000378:metabolism*; D008297:Male; D011379:Prognosis; D020543:Proteome; D012008:Recurrence; D047992:TNF Receptor-Associated Factor 2 / Q000096:biosynthesis*; D046888:Tissue Array Analysis
chemical_list: D015415:Biomarkers; D020543:Proteome; D047992:TNF Receptor-Associated Factor 2
keywords: Crohn’s disease; classification; relapse; ulcerative colitis
references: Cancer Res. 2005 Feb 1;65(3):767-79
references: 15705873
references: Anticancer Res. 2008 Nov-Dec;28(6B):3865-70
references: 19192642
references: Am J Gastroenterol. 2009 Feb;104(2):371-83
references: 19174787
references: Gastroenterol Clin North Am. 2004 Jun;33(2):251-69, ix
references: 15177537
references: Gastrointest Endosc. 2004 Oct;60(4):505-12
references: 15472670
references: Dig Liver Dis. 2008 Jul;40(7):547-53
references: 18358796
references: Am J Gastroenterol. 2008 Aug;103(8):2007-14
references: 18802997
references: Eur J Gastroenterol Hepatol. 2012 Jul;24(7):762-9
references: 22517240
references: Scand J Gastroenterol. 2006 Sep;41(9):1037-43
references: 16938716
references: Histopathology. 2006 Oct;49(4):406-10
references: 16978204
references: Proc Natl Acad Sci U S A. 1999 Aug 17;96(17):9803-8
references: 10449775
references: Inflamm Bowel Dis. 2005 Jan;11(1):16-23
references: 15674109
references: Eur J Intern Med. 2000 Aug;11(4):197-203
references: 10967507
references: Aliment Pharmacol Ther. 2007 Feb 1;25(3):247-55
references: 17217454
references: Cell Res. 2011 Jan;21(1):71-85
references: 21173796
references: Histopathology. 2002 Apr;40(4):374-6
references: 11943023
references: Gut. 2005 Mar;54(3):321-2
references: 15710974
references: Inflamm Bowel Dis. 2008 Jun;14(6):850-7
references: 18213696
references: Colorectal Dis. 2001 Jan;3(1):2-12
references: 12791013
references: Clin Lab Sci. 2007 Spring;20(2):84-8
references: 17557707
references: Med Clin North Am. 1994 Nov;78(6):1331-52
references: 7967913
references: Arch Pathol Lab Med. 2002 Jun;126(6):702-5
references: 12033959
references: Curr Protoc Immunol. 2002 Nov;Chapter 11:Unit 11.9D
references: 18432871
references: J Crohns Colitis. 2012 Feb;6 Suppl 2:S243-9
references: 22463931
references: Genes Immun. 2008 Apr;9(3):240-8
references: 18340362
references: Gastroenterology. 2010 Feb;138(2):463-8; quiz e10-1
references: 19818785
references: Inflamm Bowel Dis. 2006 Jul;12(7):551-7
references: 16804391
references: Cell Tissue Res. 2012 Jun;348(3):589-600
references: 22427119
references: PLoS One. 2012;7(5):e37139
references: 22606341
references: J Int Med Res. 2009 Jan-Feb;37(1):169-74
references: 19215687
references: Am J Gastroenterol. 2000 Apr;95(4):878-96
references: 10763932
references: Am J Gastroenterol. 2007 Oct;102(10):2294-9
references: 17608777
references: Inflamm Bowel Dis. 2010 Jul;16(7):1239-46
references: 20127998
references: Br Med J. 1964 Jan 11;1(5375):89-92
references: 14075156
references: Gastroenterology. 2000 Jul;119(1):15-22
references: 10889150
references: Gut. 2006 Mar;55(3):426-31
references: 16474109
references: Gut. 2011 May;60(5):571-607
references: 21464096
references: Gut. 2005 Mar;54(3):364-8
references: 15710984
references: Gastroenterology. 2007 Aug;133(2):412-22
references: 17681162
references: J Biol Chem. 2010 Dec 10;285(50):39511-22
references: 20937806
references: J Clin Gastroenterol. 2006 Jan;40(1):44-8
references: 16340633
references: Am J Gastroenterol. 2008 Jan;103(1):162-9
references: 17916108
references: Scand J Clin Lab Invest. 2007;67(8):810-20
references: 18034391
references: Proc Natl Acad Sci U S A. 2011 Feb 15;108(7):2903-8
references: 21282629