title: Engineering Resistance Against Mungbean yellow mosaic India virus Using Antisense RNA.
abstract: Yellow mosaic disease of cultivated legumes in South-East Asia, is caused by Mungbean yellow mosaic India virus (MYMIV) and Mungbean yellow mosaic virus (MYMV) belonging to the genus Begomovirus of the family Geminiviridae. Efforts to engineer resistance against the genus Begomovirus are focused mainly on silencing of complementary-sense virus genes involved in virus replication. Here we have targeted a complementary-sense gene (ACI) encoding Replication initiation Protein (Rep) to develop resistance against soybean isolate of Mungbean yellow mosaic India virus-[India:New Delhi:Soybean 2:1999], a bipartite begomovirus prevalent throughout the Indian subcontinent. We show that the legume host plants co-agroinoculated with infectious constructs of soybean isolate of Mungbean yellow mosaic India virus [India:New Delhi:Soybean 2:1999] along with this antisense Rep gene construct show resistance to the virus.
authors: Advanced Centre for Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110012 India.
authors: Q M I
authors: QM
authors: Haq
authors: Arif
authors: A
authors: Ali
authors: V G
authors: VG
authors: Malathi
keywords: Agroinoculation; Legumes; MYMIV isolate
references: Nat Biotechnol. 2003 Feb;21(2):131-2
references: 12560831
references: Virus Genes. 2006 Dec;33(3):365-74
references: 16991009
references: Plant Mol Biol. 2004 Nov;56(4):601-11
references: 15630623
references: Proc Natl Acad Sci U S A. 1980 Dec;77(12):7347-51
references: 7012838
references: Arch Virol. 2008;153(4):783-821
references: 18256781
references: Proc Natl Acad Sci U S A. 1991 Aug 1;88(15):6721-5
references: 1862097
references: J Virol. 2003 Jun;77(12):6785-98
references: 12767999
references: J Biol Chem. 1997 Apr 11;272(15):9840-6
references: 9092519
references: Crit Rev Biochem Mol Biol. 2000;35(2):105-40
references: 10821479
references: Plant Mol Biol. 1997 Jan;33(2):351-7
references: 9037152