title: [Metabolic effects of ganglioside GM1 on PC12 cells at oxidative stress depend on modulation of activity of tyrosine kinase of trk receptor].
abstract: Evidence has been obtained that only GM1, but also other main brain gangliosides (GD1a, GD1b, and GT1b) increase viability of ells of the PC12 neuronal line submitted to action of H2O2. By the example of GM1 and GD1a, gangliosides have been shown to induce a protective effect when acting on PC12 cells under conditions of oxidative stress both at micro- and nanomolar concentrations that are physiological concentrations of gangliosides in cerebrospinal fluid. It has been shown for the first time that GM1 at nanomolar concentrations decrease the H2O2-induced formation of reactive oxygen species (ROS). It was found that in the presence of K-252a, an inhibitor of tyrosine kinase of Trk receptors, GM1 at concentrations of 10 microM and 10 nM lost the ability to increase viability of these cells under conditions of oxidative stress. The dependence of protective and metabolic effects of ganglioside GM 1 in PC 12 cells at action on them of H2O2 on modulation of activity of tyrosine kinase of Trk receptors (i. e., on the same signal system) agrees with concept of the essential role of the GM1 antioxidant effect in its increase of cell viability.
authors: Iu A
authors: IuA
authors: Vlasova
authors: I O
authors: IO
authors: Zakharova
authors: T V
authors: TV
authors: Sokolova
authors: N F
authors: NF
authors: Avrova
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002227:Carbazoles / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D005677:G(M1) Ganglioside / Q000134:cerebrospinal fluid / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D026121:Indole Alkaloids / Q000494:pharmacology; D009474:Neurons / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D016716:PC12 Cells; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D020917:Receptor, trkA / Q000037:antagonists & inhibitors / Q000378:metabolism; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D002227:Carbazoles; D026121:Indole Alkaloids; D017382:Reactive Oxygen Species; D005677:G(M1) Ganglioside; C049985:staurosporine aglycone; D006861:Hydrogen Peroxide; D020917:Receptor, trkA; D000254:Sodium-Potassium-Exchanging ATPase