title: A mini-IRES sequence for stringent selection of high producers.
abstract: Internal Ribosome Entry Site (IRES) sequences have been widely used to link the expression of two independent proteins on the same mRNA transcript. Genes encoding fluorescent proteins or drug-resistance enzymes are usually placed downstream of IRES, serving as expression indicators or selection markers. In biological applications where the upstream gene-of-interest is to be expressed at extremely high levels, it is often desirable to purposely reduce IRES downstream gene expression to economize the cellular resources and/or to generate more stringent selection pressure. Here we describe a miniature IRES mutant sequence (IRESmut3) with dramatically diminished co-translational efficiency to fulfill these purposes.
authors: Institute of Surgery Research, State Key Laboratory of Trauma, Burns and Combined Injury, Research Institute for Traffic Medicine, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
authors: Jun
authors: J
authors: Yan
authors: Hailin
authors: H
authors: Yang
authors: Guohua
authors: G
authors: Yue
authors: Wenda
authors: W
authors: Gao
mesh_terms: D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D016466:CHO Cells; D003412:Cricetulus; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000096:biosynthesis / Q000235:genetics; D057809:HEK293 Cells; D006801:Humans; D008164:Luminescent Proteins / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D011401:Promoter Regions, Genetic; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D012270:Ribosomes / Q000502:physiology*; D017422:Sequence Analysis, DNA
chemical_list: D008164:Luminescent Proteins; D012333:RNA, Messenger; C485184:enhanced green fluorescent protein; C413663:fluorescent protein 583; D049452:Green Fluorescent Proteins
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