title: Accurate timekeeping is controlled by a cycling activator in Arabidopsis.
abstract: Transcriptional feedback loops are key to circadian clock function in many organisms. Current models of the Arabidopsis circadian network consist of several coupled feedback loops composed almost exclusively of transcriptional repressors. Indeed, a central regulatory mechanism is the repression of evening-phased clock genes via the binding of morning-phased Myb-like repressors to evening element (EE) promoter motifs. We now demonstrate that a related Myb-like protein, REVEILLE8 (RVE8), is a direct transcriptional activator of EE-containing clock and output genes. Loss of RVE8 and its close homologs causes a delay and reduction in levels of evening-phased clock gene transcripts and significant lengthening of clock pace. Our data suggest a substantially revised model of the circadian oscillator, with a clock-regulated activator essential both for clock progression and control of clock outputs. Further, our work suggests that the plant clock consists of a highly interconnected, complex regulatory network rather than of coupled morning and evening feedback loops. DOI:http://dx.doi.org/10.7554/eLife.00473.001.
authors: Department of Plant Biology , University of California, Davis , Davis , United States.
authors: Polly Yingshan
authors: PY
authors: Hsu
authors: Upendra K
authors: UK
authors: Devisetty
authors: Stacey L
authors: SL
authors: Harmer
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000502:physiology; D002940:Circadian Rhythm; D017343:Genes, Plant; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000502:physiology
chemical_list: D029681:Arabidopsis Proteins; C559753:REVEILLE 8, protein Arabidopsis; D014157:Transcription Factors
keywords: Arabidopsis; circadian rhythm; evening element; phase; transcription factors
references: Bioinformatics. 2009 Jul 15;25(14):1754-60
references: 19451168
references: Planta. 2003 Nov;218(1):159-62
references: 12955513
references: Biotechniques. 2002 Apr;32(4):728-30
references: 11962590
references: PLoS One. 2012;7(11):e49853
references: 23185460
references: J Biol Chem. 2011 Aug 5;286(31):27654-62
references: 21659538
references: Cell. 1998 Jun 26;93(7):1207-17
references: 9657153
references: Proc Natl Acad Sci U S A. 2012 Feb 21;109(8):3167-72
references: 22315425
references: Nature. 2002 Sep 5;419(6902):74-7
references: 12214234
references: Genetics. 2000 Oct;156(2):665-75
references: 11014814
references: Plant Physiol. 2010 Feb;152(2):500-15
references: 20007449
references: Plant J. 2006 Aug;47(3):480-9
references: 16813578
references: Plant Cell. 2010 Mar;22(3):594-605
references: 20233950
references: J Biol Chem. 1995 Dec 1;270(48):28995-9003
references: 7499432
references: Mol Syst Biol. 2012 Mar 06;8:574
references: 22395476
references: Curr Biol. 2002 Apr 30;12(9):757-61
references: 12007421
references: Mol Plant. 2008 Jan;1(1):27-41
references: 19802365
references: Science. 2000 Dec 15;290(5499):2110-3
references: 11118138
references: Proc Natl Acad Sci U S A. 2012 Oct 16;109(42):17123-8
references: 23027938
references: Genomics. 1997 Nov 15;46(1):24-36
references: 9403055
references: Cell. 2011 Jan 21;144(2):268-81
references: 21236481
references: Science. 1999 Sep 3;285(5433):1579-82
references: 10477524
references: Proc Natl Acad Sci U S A. 2005 Jul 19;102(29):10387-92
references: 16006522
references: Cell. 1998 Jun 26;93(7):1219-29
references: 9657154
references: Plant Physiol. 2007 Jan;143(1):473-86
references: 17098855
references: Dev Cell. 2002 May;2(5):629-41
references: 12015970
references: Annu Rev Plant Biol. 2009;60:357-77
references: 19575587
references: Plant Cell Physiol. 2003 Nov;44(11):1119-30
references: 14634148
references: Genome Biol. 2008;9(8):R130
references: 18710561
references: Plant J. 2011 Apr;66(2):318-29
references: 21205033
references: Nucleic Acids Res. 2007 Jul;35(Web Server issue):W259-64
references: 17485471
references: Proc Natl Acad Sci U S A. 1999 Mar 30;96(7):4176-9
references: 10097183
references: Adv Genet. 2011;74:175-230
references: 21924978
references: Plant Physiol. 2005 Jul;138(3):1301-9
references: 16010004
references: PLoS One. 2011;6(10):e26426
references: 22039485
references: Science. 2003 Aug 1;301(5633):653-7
references: 12893945
references: Genes Cells. 2005 Oct;10(10):963-72
references: 16164597
references: Curr Opin Plant Biol. 2012 Jun;15(3):293-300
references: 22305520
references: Nat Genet. 2005 Feb;37(2):187-92
references: 15665827
references: Nat Protoc. 2006;1(2):641-6
references: 17406292
references: Cell. 1988 Sep 23;54(7):1073-80
references: 2843290
references: Proc Natl Acad Sci U S A. 2009 Sep 29;106(39):16883-8
references: 19805390
references: Bioinformatics. 2010 Jan 1;26(1):139-40
references: 19910308
references: Science. 2000 Aug 4;289(5480):768-71
references: 10926537
references: Plant Cell. 2005 Jul;17(7):1926-40
references: 15923346
references: Proc Natl Acad Sci U S A. 2009 Feb 17;106(7):2453-8
references: 19181858
references: Curr Biol. 2011 Jan 25;21(2):120-5
references: 21236675
references: J Biol Rhythms. 1997 Jun;12(3):204-17
references: 9181432
references: J Biol Rhythms. 2008 Dec;23(6):463-71
references: 19060255
references: Science. 2001 Aug 3;293(5531):880-3
references: 11486091
references: Plant Physiol. 2005 Jun;138(2):990-7
references: 15908595
references: Mol Syst Biol. 2005;1:2005.0013
references: 16729048
references: Elife. 2012 Nov 13;1:e00011
references: 23150795
references: Plant Cell. 2005 Aug;17(8):2255-70
references: 16006578
references: Curr Biol. 2005 Jan 11;15(1):47-54
references: 15649364
references: Curr Biol. 2011 Jan 25;21(2):126-33
references: 21236673
references: Bioinformatics. 2009 Aug 15;25(16):2078-9
references: 19505943
references: EMBO J. 2003 Feb 17;22(4):935-44
references: 12574129
references: PLoS Genet. 2011 Mar;7(3):e1001350
references: 21483796
references: Science. 2012 Apr 6;336(6077):75-9
references: 22403178
references: Nature. 2011 Jul 13;475(7356):398-402
references: 21753751
references: EMBO J. 1999 Sep 1;18(17):4679-88
references: 10469647