title: Expression of extracellular matrix metalloproteinase inducer glycosylation and caveolin-1 in healthy and inflamed human gingiva.
abstract: OBJECTIVE
  Glycosylated extracellular matrix metalloproteinase inducer (EMMPRIN) is specifically associated with caveolin-1 and influences its ability to induce matrix metalloproteinases (MMPs) production. This study investigated EMMPRIN glycosylation and caveolin-1 expression in healthy and inflamed human gingival tissues, analyzed the relationship between EMMPRIN glycosylation and caveolin-1 expression, and assessed how this interaction influenced MMP-1 production.
  
  
  METHODS
  Gingival tissues were collected from 10 healthy subjects and 15 chronic periodontitis (chronic periodontitis) subjects. EMMPRIN, caveolin-1 and MMP-1 expressions were analyzed by immunohistochemistry. EMMPRIN and caveolin-1 co-localization was detected by immunofluorescence. EMMPRIN glycosylation, caveolin-1, active MMP-1 and proMMP-1 expression was assessed by Western blot.
  
  
  RESULTS
  EMMPRIN was expressed in gingival epithelial cells, inflammatory cells, endothelial and fibroblast-like cells. Strong caveolin-1 immunoreactivity was detected in gingival epithelial and endothelial cells. Double immunofluorescence studies revealed EMMPRIN and caveolin-1 co-localization in gingival epithelium, endothelial and fibroblast-like cells. Compared with healthy subjects, the chronic periodontitis group had increased high-glycoform EMMPRIN (HG-EMMPRIN) and active MMP-1 expression (p < 0.05). Active MMP-1 and proMMP-1 protein levels were positively correlated with HG-EMMPRIN levels (p < 0.05).
  
  
  CONCLUSIONS
  EMMPRIN and caveolin-1 colocalize in periodontal tissues. The increased active MMP-1 and proMMP-1 production may be associated with elevated HG-EMMPRIN levels.
authors: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST), Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China.
authors: J
authors: J
authors: Wang
authors: D
authors: D
authors: Yang
authors: C
authors: C
authors: Li
authors: S
authors: S
authors: Shang
authors: J
authors: J
authors: Xiang
mesh_terms: D000293:Adolescent; D000328:Adult; D051926:Basigin / Q000032:analysis*; D051242:Caveolin 1 / Q000032:analysis*; D055113:Chronic Periodontitis / Q000378:metabolism* / Q000473:pathology; D003774:Dental Plaque Index; D042783:Endothelial Cells / Q000737:chemistry; D004730:Endothelium, Vascular / Q000737:chemistry; D004847:Epithelial Cells / Q000737:chemistry; D005260:Female; D005347:Fibroblasts / Q000737:chemistry; D005881:Gingiva / Q000737:chemistry* / Q000166:cytology; D006031:Glycosylation; D006801:Humans; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000032:analysis; D008875:Middle Aged; D017622:Periodontal Attachment Loss / Q000145:classification; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000145:classification; D055815:Young Adult
chemical_list: C497886:BSG protein, human; D051242:Caveolin 1; D051926:Basigin; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1
keywords: Caveolin-1; EMMPRIN; MMP-1; glycosylation; periodontitis