title: [Effects of high glucose on in vitro invasiveness of human breast cancer cell line MDA-MB-435].
abstract: OBJECTIVE
  To explore the effects and underlying mechanisms of high glucose on in vitro invasiveness of human breast cancer cell line MDA-MB-435.
  
  
  METHODS
  The invasiveness of MDA-MB-435 was determined by Matrigel-coated transwell chambers. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blot were employed to analyze the cellular expression of matrix metalloproteinase-9/matrix metalloproteinase-2/E-cadherin (MMP-9/MMP-2/E-cadherin) gene/protein.
  
  
  RESULTS
  The invasive breast cancer cell numbers of each group (Glu 5.5, 11 and 25 mmol/L) were 50 ± 5, 65 ± 6 and 77 ± 3 respectively. Cellular invasion was dramatically enhanced in the Glu 11 and 25 mmol/L group compared with the 5.5 mmol/L group. The MMP-9/MMP-2 protein expression increased significantly in the Glu 11 and 25 mmol/L groups compared with 5.5 mmol/L group while high glucose (Glu 11 and 25 mmol/L group) down-regulated significantly the E-cadherin mRNA/protein expression.
  
  
  CONCLUSIONS
  High glucose can promote the in vitro invasiveness of human breast cancer cells through the altered expression of MMP-9/MMP-2/E-cadherin.
authors: Breast Diseases Center, Henan Cancer Hospital & Affiliated Cancer Hospital, Zhengzhou University, Zhengzhou 450008, China.
authors: Jun-tao
authors: JT
authors: Li
authors: Heng-wei
authors: HW
authors: Zhang
authors: Xu-hui
authors: XH
authors: Guo
authors: Xian-fu
authors: XF
authors: Sun
authors: Ya-ning
authors: YN
authors: He
authors: Chao-jun
authors: CJ
authors: Liu
authors: Shu-de
authors: SD
authors: Cui
authors: Hui
authors: H
authors: Liu
mesh_terms: D015703:Antigens, CD; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology*; D015820:Cadherins / Q000378:metabolism; D045744:Cell Line, Tumor; D005260:Female; D005947:Glucose / Q000009:adverse effects*; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009361:Neoplasm Invasiveness
chemical_list: D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9; D005947:Glucose