title: The effects of linear assembly of two carbazole groups on acid-base and DNA-binding properties of a ruthenium(II) complex.
abstract: A novel Ru(II) complex of [Ru(bpy)2(Hbcpip)](ClO4)2 {where bpy=2,2-bipyridine, Hbcpip=2-(4-(9H-3,9'-bicarbazol-9-yl)phenyl)-1H-imidazo[4,5-f][1,10]phenanthroline} is synthesized and characterized. Calf-thymus DNA-binding properties of the complex were studied by UV-vis absorption and luminescence titrations, steady-state emission quenching by [Fe(CN)6](4-), DNA competitive binding with ethidium bromide, thermal denaturation and DNA viscosity measurements. The results indicate that the complex partially intercalated into the DNA with a binding constant of (5.5±1.4)×10(5) M(-1) in buffered 50 mM NaCl. The acid-base properties of the complex were also studied by UV-visible and luminescence spectrophotometric pH titrations, and ground- and excited-state acidity ionization constant values were derived.
authors: College of Chemistry and Beijing Key Laboratory of Energy Conversion and Storage Materials, Beijing Normal University, Beijing 100875, China.
authors: Xi
authors: X
authors: Chen
authors: Long-Xin
authors: LX
authors: Xue
authors: Chun-Chuan
authors: CC
authors: Ju
authors: Ke-Zhi
authors: KZ
authors: Wang
mesh_terms: D000042:Absorption; D000143:Acids / Q000737:chemistry*; D000468:Alkalies / Q000737:chemistry*; D000818:Animals; D002227:Carbazoles / Q000737:chemistry*; D002417:Cattle; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004583:Electrons; D004996:Ethidium / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D049449:Luminescence; D009691:Nucleic Acid Denaturation / Q000187:drug effects; D012428:Ruthenium / Q000737:chemistry / Q000378:metabolism*; D012965:Sodium Chloride / Q000494:pharmacology; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D017186:Titrimetry; D014783:Viscosity
chemical_list: D000143:Acids; D000468:Alkalies; D002227:Carbazoles; D056831:Coordination Complexes; C041514:carbazole; D012965:Sodium Chloride; D012428:Ruthenium; D004247:DNA; C427203:calf thymus DNA; D004996:Ethidium