title: Host IQGAP1 and Ebola virus VP40 interactions facilitate virus-like particle egress.
abstract: We have identified host IQGAP1 as an interacting partner for Ebola virus (EBOV) VP40, and its expression is required for EBOV VP40 virus-like particle (VLP) budding. IQGAP1 is involved in actin cytoskeletal remodeling during cell migration and formation of filopodia. The physical interaction and the functional requirement for IQGAP1 in EBOV VP40 VLP egress link virus budding to the cytoskeletal remodeling machinery. Consequently, this interaction represents a novel target for development of therapeutics to block budding and transmission of filoviruses.
authors: Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
authors: Jianhong
authors: J
authors: Lu
authors: Yonggang
authors: Y
authors: Qu
authors: Yuliang
authors: Y
authors: Liu
authors: Rakesh
authors: R
authors: Jambusaria
authors: Ziying
authors: Z
authors: Han
authors: Gordon
authors: G
authors: Ruthel
authors: Bruce D
authors: BD
authors: Freedman
authors: Ronald N
authors: RN
authors: Harty
mesh_terms: D015153:Blotting, Western; D029043:Ebolavirus / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D040461:Gene Components; D057809:HEK293 Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D014763:Viral Matrix Proteins / Q000378:metabolism*; D014771:Virion / Q000378:metabolism; D057074:Virus Release / Q000235:genetics / Q000502:physiology*; D020703:ras GTPase-Activating Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C101203:IQ motif containing GTPase activating protein 1; D034741:RNA, Small Interfering; C080981:VP40 protein, virus; D014763:Viral Matrix Proteins; D020703:ras GTPase-Activating Proteins
references: Cell Microbiol. 2007 Apr;9(4):939-51
references: 17140405
references: Adv Virol. 2011 Jan 1;2011:
references: 22102845
references: J Cell Sci. 2005 May 15;118(Pt 10):2085-92
references: 15890984
references: Virology. 2006 Jan 5;344(1):64-70
references: 16364737
references: EMBO J. 2006 May 17;25(10):2155-66
references: 16628219
references: Virology. 2011 Mar 30;412(1):68-74
references: 21262517
references: ScientificWorldJournal. 2010 May 18;10:944-53
references: 20495773
references: Arch Virol Suppl. 1996;11:77-100
references: 8800808
references: Cell Signal. 2012 Apr;24(4):826-34
references: 22182509
references: J Virol. 2006 Sep;80(18):9039-52
references: 16940516
references: J Virol. 2003 Feb;77(3):1812-9
references: 12525615
references: Virology. 2008 Mar 15;372(2):221-32
references: 18063004
references: Virol J. 2006 May 23;3:31
references: 16719918
references: EMBO Rep. 2007 Nov;8(11):1019-23
references: 17972901
references: Virus Res. 2004 Dec;106(2):181-8
references: 15567496
references: Curr Protoc Cell Biol. 2006 Jan;Chapter 21:Unit 21.3
references: 18228482
references: EMBO J. 2007 Oct 3;26(19):4215-27
references: 17853893
references: Nat Rev Mol Cell Biol. 2006 Jun;7(6):449-56
references: 16625152
references: Future Virol. 2010 Jul 1;5(4):481-491
references: 20730024
references: J Virol. 2007 Oct;81(20):11452-60
references: 17699576
references: J Virol. 2005 Aug;79(16):10300-7
references: 16051823
references: Proc Natl Acad Sci U S A. 2000 Dec 5;97(25):13871-6
references: 11095724
references: FEBS Lett. 2011 Mar 9;585(5):723-9
references: 21295032
references: J Infect Dis. 2011 Nov;204 Suppl 3:S817-24
references: 21987757
references: Histol Histopathol. 2012 Nov;27(11):1385-94
references: 23018238