title: Stealth cationic liposomes modified with anti-CAGE single-chain fragment variable deliver recombinant methioninase for gastric carcinoma therapy.
abstract: Stealth cationic liposomes (SCLs) modified with tumor-targeting single-chain fragment variable (scFV) antibody for systemic delivery of recombinant methioninase (rMETase) for gastric cancer were prepared successfully. These functional SCL nanoparticles are composed of cationic lipids, dioleoylphosphatidylethanolamine, and distearoylphosphatidylethanolamine-polyethylene glycol, which have lower gene transfection efficiencies compared with Lipofectamine 2000, and can also be used as effective gene delivery vectors. Increased therapeutic efficiency of rMETase-loaded scFV-SCLs were tested in SGC-7901 cells and compared with free rMETase in solution and rMETase-loaded SCLs. In addition, scFV-SCLs (effective diameter: 185.7 nm; polydispersity index: 0.236) can significantly boost rhodamine 123 cellular accumulation in the cytoplasm. The scFV-targeted SCLs can be used as a potentially effective drug delivery system.
authors: Department of General Surgery, The Second Affiliated Hospital of Nanchang University, 330006 Nanchang, Jiangxi, China.
authors: Lin
authors: L
authors: Xin
authors: Jiaqing
authors: J
authors: Cao
authors: Hua
authors: H
authors: Cheng
authors: Fei
authors: F
authors: Zeng
authors: Xiaoyun
authors: X
authors: Hu
mesh_terms: D013437:Carbon-Sulfur Lyases / Q000008:administration & dosage* / Q000737:chemistry; D002412:Cations; D045744:Cell Line, Tumor; D004058:Diffusion; D006801:Humans; D008081:Liposomes / Q000737:chemistry*; D053769:Nanocapsules / Q000008:administration & dosage* / Q000737:chemistry; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000737:chemistry; D012697:Serine Endopeptidases / Q000493:pharmacokinetics* / Q000627:therapeutic use; D057127:Single-Chain Antibodies / Q000493:pharmacokinetics* / Q000627:therapeutic use; D013274:Stomach Neoplasms / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology; D016896:Treatment Outcome
chemical_list: D002412:Cations; D008081:Liposomes; D053769:Nanocapsules; D011994:Recombinant Proteins; D057127:Single-Chain Antibodies; D012697:Serine Endopeptidases; C054542:chymostatin-sensitive angiotensin II-generating enzyme; D013437:Carbon-Sulfur Lyases; C021558:L-methionine gamma-lyase