title: Regulation of the heat stress response in Arabidopsis by MPK6-targeted phosphorylation of the heat stress factor HsfA2.
abstract: So far little is known on the functional role of phosphorylation in the heat stress response of plants. Here we present evidence that heat stress activates the Arabidopsis mitogen-activated protein kinase MPK6. In vitro and in vivo evidence is provided that MPK6 specifically targets the major heat stress transcription factor HsfA2. Activation of MPK6 results in complex formation with HsfA2. MPK6 phosphorylates HsfA2 on T249 and changes its intracellular localisation. Protein kinase and phosphatase inhibitor studies indicate that HsfA2 protein stability is regulated in a phosphorylation-dependent manner, but this mechanism is independent of MPK6. Overall, our data show that heat stress-induced targeting of HsfA2 by MPK6 participates in the complex regulatory mechanism how plants respond to heat stress.
authors: URGV Plant Genomics Laboratory, Evry, France.
authors: Alexandre
authors: A
authors: Evrard
authors: Department of Plant Molecular Biology, Max F. Perutz Laboratories, University of Vienna, Vienna, Austria.
authors: Mukesh
authors: M
authors: Kumar
authors: UMR Ecophysiologie et Génomique Fonctionnelle de la Vigne, France.
authors: David
authors: D
authors: Lecourieux
authors: Pharmazentrum frankfurt/ZAFES, Institute of Clinical Pharmacology, Goethe-University, Frankfurt, Germany.
authors: Jessica
authors: J
authors: Lucks
authors: Institute of Molecular Biosciences, Biocenter N200/R306, Goethe University, Frankfurt, Germany.
authors: Pascal
authors: P
authors: von Koskull-Döring
authors: URGV Plant Genomics Laboratory, Evry, France.
authors: Heribert
authors: H
authors: Hirt
keywords: Arabidopsis; Heat stress proteins; Heat stress transcription factors; Kinases; MAP kinase
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