title: Assessment of β-carotene content, cell physiology and morphology of the yellow yeast Rhodotorula glutinis mutant 400A15 using flow cytometry.
abstract: Flow cytometry was used to assess β-carotene content, cell membrane permeability, cell size and granularity in Rhodotorula glutinis mutant 400A15 grown under different oxygen transfer coefficients (k L a) and carbon to nitrogen ratios (C/N). A Doehlert distribution was used in order to select the best conditions that induced the highest carotenoids production. The highest β-carotene content (0.79 mg g(-1) DCW) at the lowest k L a and C/N (5 × 10(-3) s(-1) and 11.3 respectively). Under these conditions, the biomass concentration attained 18.60 g L(-1). The highest ratio of cells with permeabilised membranes (2.6 %), and the highest cell size and granularity were also obtained under these conditions. It was observed that C/N showed a stronger influence than the k L a on the measured cell parameters.
authors: Dipartamento di Agraria, Università degli Studi di Sassari, Viale Italia 39, 07100, Sassari, Italy.
authors: Raffaela
authors: R
authors: Cutzu
authors: Ana
authors: A
authors: Clemente
authors: Alberto
authors: A
authors: Reis
authors: Beatriz
authors: B
authors: Nobre
authors: Ilaria
authors: I
authors: Mannazzu
authors: José
authors: J
authors: Roseiro
authors: Teresa
authors: T
authors: Lopes da Silva
mesh_terms: D018533:Biomass; D002244:Carbon / Q000378:metabolism; D005434:Flow Cytometry; D009154:Mutation; D009584:Nitrogen / Q000378:metabolism; D012248:Rhodotorula / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D019207:beta Carotene / Q000032:analysis / Q000096:biosynthesis*
chemical_list: D019207:beta Carotene; D002244:Carbon; D009584:Nitrogen
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