title: Highly sensitive and selective colorimetric sensing of antibiotics in milk.
abstract: Antibiotics residues in foods are very harmful to human beings. Determination of antibiotics residues relies largely on the availability of adequate analytical techniques. Currently, there is an urgent need for on site and real time detection of antibiotics in food. In this work, a novel one step synthesis of gold nanoparticles (AuNPs) was proposed using pyrocatechol violet (PCV) as a reducer agent. Highly sensitive and selective colorimetric detection of four antibiotics kanamycin mono sulfate (KA), neomycin sulfate (NE), streptomycin sulfate (ST) and bleomycin sulfate (BL) was realized during the formation of AuNPs. PCV has -OH groups and these antibiotics have -OH, -NH2, -NH- groups, so there may be some special hydrogen-bonding interactions between PCV and these antibiotics. Therefore, the presence of KA, NE, ST and BL would influence the synthesis of AuNPs, then the color and state of AuNPs would change, which could be observed with the naked eye or a UV-vis spectrophotometer. Results showed that A670 was linear with the logarithm of KA concentration in the range from 1.0×10(-8) to 5.0×10(-7)M and 5.0×10(-7) to 5.5×10(-5)M. The detection limit of KA was 1.0×10(-9)M (S/N=3). The coexisting substances including 1.0×10(-5)M phenylalanine, alanine, glycerol, glucose, Mg(2+), Ca(2+), Na(+), K(+), CO3(2-), SO4(2-), NO3(-), Cl(-) and Br(-) did not affect the determination of 1.0×10(-7)M antibiotics. In particular, the proposed method could be applied successfully to the detection of antibiotics in the pretreated liquid milk products.
authors: School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, Beijing, China.
authors: Xiaofang
authors: X
authors: Zhang
authors: Yang
authors: Y
authors: Zhang
authors: Hong
authors: H
authors: Zhao
authors: Yujian
authors: Y
authors: He
authors: Xiangjun
authors: X
authors: Li
authors: Zhuobin
authors: Z
authors: Yuan
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D003124:Colorimetry; D005504:Food Analysis / Q000379:methods*; D006046:Gold / Q000737:chemistry; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D008892:Milk / Q000737:chemistry*; D013057:Spectrum Analysis
chemical_list: D000900:Anti-Bacterial Agents; D006046:Gold