title: Genetic relationship between multiple squamous cell carcinomas arising in the oral cavity.
abstract: BACKGROUND
  Histological and clinical criteria are generally used to differentiate second primary tumors (SPTs) from local recurrences. The purpose of the present study was to apply mitochondrial DNA (mtDNA) D-loop analysis to differentiate SPTs from local recurrences and to validate the clinical classification.
  
  
  METHODS
  The study population consisted of 20 consecutive patients presenting multiple oral neoplastic lesions for a total of 25 paired lesions. The mtDNA D-loop analysis was performed by direct sequencing and phylogenetic clusterization.
  
  
  RESULTS
  Agreement between mtDNA analysis and clinical classification was found in 19 cases. Discrepancies arose in 6 cases in which the clinical criteria based only on the spatial or temporal distance of the second lesion from the index tumor had led to a diagnosis of SPT (2 cases) or local recurrence (4 cases).
  
  
  CONCLUSIONS
  The present data highlight the value of mtDNA analysis in establishing the clonal relationship between the index tumor and the second neoplastic lesion.
authors: Section of Oral Science, Department of Biomedical and Neuro-Muscular Sciences, University of Bologna, Bologna, Italy.
authors: Lucio
authors: L
authors: Montebugnoli
authors: Elisa
authors: E
authors: Leonardi
authors: Luca
authors: L
authors: Morandi
authors: Anna
authors: A
authors: Farnedi
authors: Davide Bartolomeo
authors: DB
authors: Gissi
authors: Claudio
authors: C
authors: Marchetti
authors: Achille
authors: A
authors: Tarsitano
authors: Tiziana
authors: T
authors: Balbi
authors: Luciano
authors: L
authors: Gentile
authors: Roberto
authors: R
authors: Cocchi
authors: Maria Pia
authors: MP
authors: Foschini
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D015331:Cohort Studies; D004252:DNA Mutational Analysis; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D005500:Follow-Up Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D009364:Neoplasm Recurrence, Local / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D016609:Neoplasms, Second Primary / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D012189:Retrospective Studies; D018570:Risk Assessment
chemical_list: D004272:DNA, Mitochondrial
keywords: clonality; mitochondrial DNA D-loop; multiple tumors; oral cancer; second primary tumor