title: T cells expressing a chimeric antigen receptor that binds hepatitis B virus envelope proteins control virus replication in mice.
abstract: OBJECTIVE
  Antiviral agents suppress hepatitis B virus (HBV) replication but do not clear the infection. A strong effector T-cell response is required to eradicate HBV, but this does not occur in patients with chronic infection. T cells might be directed toward virus-infected cells by expressing HBV-specific receptors and thereby clear HBV and help to prevent development of liver cancer. In mice, we studied whether redirected T cells can engraft after adoptive transfer, without prior T-cell depletion, and whether the large amounts of circulating viral antigens inactivate the transferred T cells or lead to uncontrolled immune-mediated damage.
  
  
  METHODS
  CD8(+) T cells were isolated from mice and stimulated using an optimized protocol. Chimeric antigen receptors (CARs) that bind HBV envelope proteins (S-CAR) and activate T cells were expressed on the surface of cells using retroviral vectors. S-CAR-expressing CD8(+) T cells, which carried the marker CD45.1, were injected into CD45.2(+) HBV transgenic mice. We compared these mice with mice that received CD8(+) T cells induced by vaccination, cells that express a CAR without a proper signaling domain, or cells that express a CAR that does not bind HBV proteins (controls).
  
  
  RESULTS
  CD8(+) T cells that expressed HBV-specific CARs recognized different HBV subtypes and were able to engraft and expand in immune-competent HBV transgenic mice. After adoptive transfer, the S-CAR-expressing T cells localized to and functioned in the liver and rapidly and efficiently controlled HBV replication compared with controls, causing only transient liver damage. The large amount of circulating viral antigen did not impair or overactivate the S-CAR-grafted T cells.
  
  
  CONCLUSIONS
  T cells with a CAR specific for HBV envelope proteins localize to the liver in mice to reduce HBV replication, causing only transient liver damage. This immune cell therapy might be developed for patients with chronic hepatitis B, regardless of their HLA type.
authors: Institute of Virology, Technische Universität München/Helmholtz Zentrum München, München, Germany.
authors: Karin
authors: K
authors: Krebs
authors: Nina
authors: N
authors: Böttinger
authors: Li-Rung
authors: LR
authors: Huang
authors: Markus
authors: M
authors: Chmielewski
authors: Silke
authors: S
authors: Arzberger
authors: Georg
authors: G
authors: Gasteiger
authors: Clemens
authors: C
authors: Jäger
authors: Edgar
authors: E
authors: Schmitt
authors: Felix
authors: F
authors: Bohne
authors: Michaela
authors: M
authors: Aichler
authors: Wolfgang
authors: W
authors: Uckert
authors: Hinrich
authors: H
authors: Abken
authors: Mathias
authors: M
authors: Heikenwalder
authors: Percy
authors: P
authors: Knolle
authors: Ulrike
authors: U
authors: Protzer
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D006515:Hepatitis B virus / Q000276:immunology* / Q000502:physiology; D006801:Humans; D008099:Liver / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D011993:Recombinant Fusion Proteins / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology*; D014779:Virus Replication / Q000276:immunology*
chemical_list: D011948:Receptors, Antigen, T-Cell; D011993:Recombinant Fusion Proteins; D014759:Viral Envelope Proteins
keywords: ALT; Adoptive T-Cell Therapy; CAR; CEA; Chronic Hepatitis B; DC; HBV; HBVtg; HBsAg; HCC; Hepatocellular Carcinoma; IFN; IL; Immunotherapy; S-CAR; TNF; alanine aminotransferase; carcinoembryonic antigen; cccDNA; chimeric antigen receptor; covalently closed circular DNA; dendritic cells; hepatitis B surface antigen; hepatitis B virus; hepatitis B virus transgenic; hepatitis B virus–specific chimeric antigen receptor; hepatocellular carcinoma; interferon; interleukin; scFv; single-chain fragment variable; tumor necrosis factor