title: Arabidopsis thaliana: proliferating cell nuclear antigen 1 and 2 possibly form homo- and hetero-trimeric complexes in the plant cell.
abstract: The proliferating cell nuclear antigen (PCNA) is a key component of the eukaryotic DNA replication machinery. It also plays an important role in DNA repair mechanisms. Despite the intense scientific research on yeast and human PCNA, information describing the function of this protein in plants is still very limited. In the previous study Arabidopsis PCNA2 but not PCNA1 was proposed to be functionally important in DNA polymerase η-dependent postreplication repair. In addition to the above study, PCNA2 but not PCNA1 was also shown to be necessary for Arabidopsis DNA polymerase λ-dependent oxidative DNA damage bypass. Taking into account the reported differences between PCNA1 and PCNA2, we tested the idea of a possible cooperation between PCNA1 and PCNA2 in the plant cell. In a bimolecular fluorescence complementation assay an interaction between PCNA1 and PCNA2 was observed in the nucleus, as well as in the cytoplasm. This finding, together with our previous results, indicates that PCNA1 and PCNA2 may cooperate in planta by forming homo- and heterotrimeric rings. The observed interaction might be relevant when distinct functions for PCNA1 and PCNA2 are considered.
authors: Department of Plant Biotechnology; Faculty of Biochemistry; Biophysics and Biotechnology; Jagiellonian University; Krakow, Poland.
authors: Wojciech
authors: W
authors: Strzalka
authors: Chhavi
authors: C
authors: Aggarwal
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D049109:Cell Proliferation; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism*; D055503:Protein Multimerization
chemical_list: D029681:Arabidopsis Proteins; D018809:Proliferating Cell Nuclear Antigen
keywords: Arabidopsis thaliana; DNA repair; DNA replication; PCNA; cell cycle
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