title: Influence of limonin on Wnt signalling molecule in HepG2 cell lines.
abstract: OBJECTIVE
  The role of limonin as potent anti carcinogenic, apoptosis and chemotherapeutic agents has been supported by limited studies.
  
  
  METHODS
  In this study, limonin is identified as a potent anti proliferative agent against human hepatoma HepG2 cells based on the cell viability study, LDH leakage assay. Induction of apoptosis in HepG2 cells by limonin was evidenced by western blot analysis of Bax, Cyclin D1, Caspase 3 and Caspase9.
  
  
  RESULTS
  Since Wnt signalling is involved in the initiation and sustaining of hepatocellular carcinoma we studied differential expression of LRP5, LRP6 and DKK wnt players.
  
  
  CONCLUSIONS
  Limonin found to down regulate these players which forms a rationale for further investigation on effect on limonin in cancer therapy.
authors: Department of Industrial Biotechnology, Bharath University, Tambaram, India.
authors: K
authors: K
authors: Langeswaran
authors: S
authors: S
authors: Gowthamkumar
authors: S
authors: S
authors: Vijayaprakash
authors: R
authors: R
authors: Revathy
authors: M P
authors: MP
authors: Balasubramanian
keywords: Apoptosis; Wnt; caspases; hepatocellular carcinoma; limonin; β-catenin
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