title: The effect of Laminaria japonic polysaccharide on sperm characteristics and biochemical parameters in cryopreserved boar sperm.
abstract: The aim of the present study was to evaluate the cryoprotective effect of Laminaria japonic polysaccharide (LJP) on boar sperm. Semen samples were collected from seven mature Yorkshire boars once a week by the gloved hand technique and frozen-thawed in the extender with LJP added. Extender with LJP added at concentrations of 0.25, 0.5, 1.0, 1.5 and 2.0mg/mL to the extender and its effects on the quality of frozen-thawed boar sperm were assessed. Results showed: (i) sperm motility and plasma membrane integrity were greater in the extender containing 0.5 and 1.0mg/mL LJP, as compared to other groups (P<0.05); (ii) extender added 1.0mg/mL LJP showed the greatest plasma membrane and acrosomal integrity percentages in comparison with other groups (P<0.05); (iii) mitochondrial activity was significantly higher at the concentration of 0.5 and 1.0mg/mL LJP than those of other groups (P<0.05); (iv) in terms of biochemical assessments, 0.5 and 1.0mg/mL LJP improved SOD (superoxide dismutase) and CAT (catalase) concentrations, compared to other groups (P<0.05). However, no significant difference was found in GSH-Px (glutathione peroxidase) concentration when supplemented with LJP. Interestingly, LJP exhibited a dose-related response and the lesser concentration represented greater protective effects. It is also important to note that 1.0mg/mL LJP provides for an enhanced cryoprotective effect in boar semen.
authors: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi Province 712100, PR China.
authors: Jing-Hua
authors: JH
authors: Hu
authors: Xiu-Zhu
authors: XZ
authors: Sun
authors: Qing-Wang
authors: QW
authors: Li
authors: Ting
authors: T
authors: Zhang
authors: Xiao-Chen
authors: XC
authors: Hu
authors: Jian-Hong
authors: JH
authors: Hu
authors: Li-Qiang
authors: LQ
authors: Wang
mesh_terms: D000818:Animals; D002374:Catalase / Q000378:metabolism; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary*; D005979:Glutathione Peroxidase / Q000378:metabolism; D007795:Laminaria / Q000737:chemistry; D008297:Male; D011134:Polysaccharides / Q000494:pharmacology*; D055101:Semen Analysis / Q000662:veterinary; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013094:Spermatozoa / Q000187:drug effects* / Q000201:enzymology / Q000502:physiology*; D013482:Superoxide Dismutase / Q000378:metabolism; D013552:Swine / Q000502:physiology*
chemical_list: D011134:Polysaccharides; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase