title: Mitogen-inducible Gene-2 (MIG2) and migfilin expression is reduced in samples of human breast cancer.
abstract: BACKGROUND
  Cell adhesion proteins that connect each cell to neighboring cells and the extracellular matrix play a fundamental role in metastasis. Mitogen-inducible gene-2 (MIG2), is a cell-matrix adhesion protein, which through migfilin, interacts with filamin-A, being linked to actin cytoskeleton.
  
  
  OBJECTIVE
  Recent studies have implicated both MIG2 and migfilin in cancer, but little is known regarding their expression in breast cancer. In this study, we investigated this topic.
  
  
  METHODS
  mRNA and protein expression was examined in 30 breast cancer samples and compared to that of normal adjacent tissue using real time-polymerase chain reaction (PCR) and western blotting.
  
  
  RESULTS
  Our results showed that expression of MIG2 and migfilin was significantly reduced in the majority of the breast cancer tissues compared to normal tissues regardless of metastatic status and disease stage.
  
  
  CONCLUSIONS
  Both MIG2 and migfilin are down-regulated in breast cancer.
authors: Department of Biomedical Research and Technology, Institute for Research and Technology-Thessaly (I.RE.TE.TH), 51 Papanastasiou street, Larissa 41222, Greece. vasso.gkretsi@gmail.com
authors: Vasiliki
authors: V
authors: Gkretsi
authors: Vassilis
authors: V
authors: Papanikolaou
authors: Lefteris C
authors: LC
authors: Zacharia
authors: Evangelos
authors: E
authors: Athanassiou
authors: Chuanyue
authors: C
authors: Wu
authors: Aspasia
authors: A
authors: Tsezou
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D001940:Breast / Q000378:metabolism* / Q000473:pathology; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000235:genetics / Q000378:metabolism / Q000556:secondary; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000235:genetics / Q000378:metabolism / Q000556:secondary; D018275:Carcinoma, Lobular / Q000235:genetics / Q000378:metabolism / Q000556:secondary; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules; D003598:Cytoskeletal Proteins; C473794:FBLIM1 protein, human; C477322:FERMT3 protein, human; D008565:Membrane Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger
keywords: Breast cancer; MIG2; migfilin; mitogen-inducible gene-2