title: Delineating antibody recognition in polyclonal sera from patterns of HIV-1 isolate neutralization.
abstract: Serum characterization and antibody isolation are transforming our understanding of the humoral immune response to viral infection. Here, we show that epitope specificities of HIV-1-neutralizing antibodies in serum can be elucidated from the serum pattern of neutralization against a diverse panel of HIV-1 isolates. We determined "neutralization fingerprints" for 30 neutralizing antibodies on a panel of 34 diverse HIV-1 strains and showed that similarity in neutralization fingerprint correlated with similarity in epitope. We used these fingerprints to delineate specificities of polyclonal sera from 24 HIV-1-infected donors and a chimeric siman-human immunodeficiency virus-infected macaque. Delineated specificities matched published specificities and were further confirmed by antibody isolation for two sera. Patterns of virus-isolate neutralization can thus afford a detailed epitope-specific understanding of neutralizing-antibody responses to viral infection.
authors: Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
authors: Ivelin S
authors: IS
authors: Georgiev
authors: Nicole A
authors: NA
authors: Doria-Rose
authors: Tongqing
authors: T
authors: Zhou
authors: Young Do
authors: YD
authors: Kwon
authors: Ryan P
authors: RP
authors: Staupe
authors: Stephanie
authors: S
authors: Moquin
authors: Gwo-Yu
authors: GY
authors: Chuang
authors: Mark K
authors: MK
authors: Louder
authors: Stephen D
authors: SD
authors: Schmidt
authors: Han R
authors: HR
authors: Altae-Tran
authors: Robert T
authors: RT
authors: Bailer
authors: Krisha
authors: K
authors: McKee
authors: Martha
authors: M
authors: Nason
authors: Sijy
authors: S
authors: O'Dell
authors: Gilad
authors: G
authors: Ofek
authors: Marie
authors: M
authors: Pancera
authors: Sanjay
authors: S
authors: Srivatsan
authors: Lawrence
authors: L
authors: Shapiro
authors: Mark
authors: M
authors: Connors
authors: Stephen A
authors: SA
authors: Migueles
authors: Lynn
authors: L
authors: Morris
authors: Yoshiaki
authors: Y
authors: Nishimura
authors: Malcolm A
authors: MA
authors: Martin
authors: John R
authors: JR
authors: Mascola
authors: Peter D
authors: PD
authors: Kwong
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood / Q000276:immunology*; D018604:Epitope Mapping; D015483:HIV Antibodies / Q000097:blood / Q000276:immunology*; D015658:HIV Infections / Q000097:blood / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D016056:Immunodominant Epitopes / Q000737:chemistry / Q000276:immunology; D008251:Macaca; D009500:Neutralization Tests; D011487:Protein Conformation; D044967:Serum / Q000276:immunology
chemical_list: D057134:Antibodies, Neutralizing; D015483:HIV Antibodies; D016056:Immunodominant Epitopes