title: Hydrogen peroxide enhances radiation-induced apoptosis and inhibition of melanoma cell proliferation.
abstract: The efficacy of radiation therapy (RT) for melanoma is limited in part by its radioresistance. Here, we examined the radiosensitizing effect of hydrogen peroxide (H(2)O(2)) on a radioresistant melanoma cell line, HTB-65. We found that H(2)O(2) synergized with RT to inhibit melanoma cell proliferation and promote apoptosis. The antiproliferative effect of H(2)O(2)/RT correlated with increased expression of p15 and reduced expression of cyclin D, cyclin E, cyclin-dependent kinase (CDK)2 and CDK4. The pro-apoptotic effect of H(2)O(2) /RT correlated with reduced expression of the B-cell CLL/lymphoma (BCL)2. These data highlight the potential of H(2)O(2) as a radiation sensitizer for melanoma treatment and show that this warrants further study.
authors: Department of Surgery, Ellis Fischel Cancer Center, University of Missouri School of Medicine, M316, Medical Sciences Building, Columbia, MO, 65212, USA. fangy@health.missouri.edu
authors: Yujiang
authors: Y
authors: Fang
authors: Bradley J
authors: BJ
authors: Moore
authors: Qian
authors: Q
authors: Bai
authors: Kathryn M
authors: KM
authors: Cook
authors: Elizabeth J
authors: EJ
authors: Herrick
authors: Michael B
authors: MB
authors: Nicholl
mesh_terms: D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects; D015153:Blotting, Western; D049109:Cell Proliferation / Q000187:drug effects* / Q000528:radiation effects; D019008:Drug Resistance, Neoplasm / Q000187:drug effects* / Q000528:radiation effects; D005720:Gamma Rays; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007124:Immunoenzyme Techniques; D008545:Melanoma / Q000188:drug therapy / Q000473:pathology* / Q000532:radiotherapy; D016877:Oxidants / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: D016877:Oxidants; D012333:RNA, Messenger; D006861:Hydrogen Peroxide
keywords: Hydrogen peroxide; apoptosis; melanoma; proliferation; radiation