title: A combination of two electrophoretical approaches for detailed proteome-based characterization of SCLC subtypes.
abstract: BACKGROUND
  Small cell lung cancers (SCLC) are heterogeneous and tumours differ in growth characteristics and treatment resistance.
  
  
  OBJECTIVE
  To get insight into the underlying protein profiles responsible for this heterogeneity, two subtypes of SCLC cells mutually differing in chemo resistance properties and growth characteristics are analysed.
  
  
  METHODS
  Two different electrophoresis approaches in combination with mass spectrometry were used to detect differences between the SCLC cell lines GLC1 and GLC1M13: IEF/SDS-PAGE as well as cetyltrimethylammonium bromide (CTAB)-SDS-PAGE.
  
  
  RESULTS
  Altogether 60 non redundant differentially expressed proteins were found of which 5 were verified by Western Blot analysis.
  
  
  CONCLUSIONS
  Most of these proteins identified are involved in processes of tumour progression. Therefore, these proteins are interesting candidates for further functional analysis.
  
  
  CONCLUSIONS
  Additional CTAB-SDS page is a complementary method to IEF-SDS page revealing a complete new subset of proteins differentially expressed between GLC1 and GLC1 M13 cells SCLC subtypes.
authors: Molecular Proteomics Laboratory, BMFZ, Universität Düsseldorf, Germany. gereon.poschmann@hhu.de
authors: Gereon
authors: G
authors: Poschmann
authors: Anna
authors: A
authors: Lendzian
authors: Julian
authors: J
authors: Uszkoreit
authors: Martin
authors: M
authors: Eisenacher
authors: Ann Vander
authors: AV
authors: Borght
authors: Frans C
authors: FC
authors: Ramaekers
authors: Helmut Erich
authors: HE
authors: Meyer
authors: Kai
authors: K
authors: Stühler
mesh_terms: D015153:Blotting, Western; D045744:Cell Line, Tumor; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry*; D019008:Drug Resistance, Neoplasm; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007525:Isoelectric Focusing; D008175:Lung Neoplasms / Q000737:chemistry* / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D058977:Molecular Sequence Annotation; D009363:Neoplasm Proteins / Q000032:analysis* / Q000235:genetics; D040901:Proteomics; D055752:Small Cell Lung Carcinoma / Q000737:chemistry* / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D002593:Cetrimonium Compounds; D009363:Neoplasm Proteins; D000077286:Cetrimonium