title: Cotton AnnGh3 encoding an annexin protein is preferentially expressed in fibers and promotes initiation and elongation of leaf trichomes in transgenic Arabidopsis.
abstract: The annexins are a multifamily of calcium-regulated phospholipid-binding proteins. To investigate the roles of annexins in fiber development, four genes encoding putative annexin proteins were isolated from cotton (Gossypium hirsutum) and designated AnnGh3, AnnGh4, AnnGh5, and AnnGh6. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) results indicated that AnnGh3, AnnGh4, and AnnGh5 were preferentially expressed in fibers, while the transcripts of AnnGh6 were predominantly accumulated in roots. During fiber development, the transcripts of AnnGh3/4/5 genes were mainly accumulated in rapidly elongating fibers. With fiber cells further developed, their expression activity was dramatically declined to a relatively low level. In situ hybridization results indicated that AnnGh3 and AnnGh5 were expressed in initiating fiber cells (0-2 DPA). Additionally, their expression in fibers was also regulated by phytohormones and [Ca(2+)]. Subcellular localization analysis discovered that AnnGh3 protein was localized in the cytoplasm. Overexpression of AnnGh3 in Arabidopsis resulted in a significant increase in trichome density and length on leaves of the transgenic plants, suggesting that AnnGh3 may be involved in fiber cell initiation and elongation of cotton.
authors: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan, 430079, China.
authors: Bing
authors: B
authors: Li
authors: Deng-Di
authors: DD
authors: Li
authors: Jie
authors: J
authors: Zhang
authors: Hui
authors: H
authors: Xia
authors: Xiu-Lan
authors: XL
authors: Wang
authors: Ying
authors: Y
authors: Li
authors: Xue-Bao
authors: XB
authors: Li
mesh_terms: D000595:Amino Acid Sequence; D017302:Annexins / Q000235:genetics / Q000378:metabolism*; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D002118:Calcium / Q000494:pharmacology; D032561:Cotton Fiber; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D003368:Gossypium / Q000166:cytology / Q000187:drug effects / Q000235:genetics*; D007477:Ions; D008969:Molecular Sequence Data; D056771:Ovule / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D034443:RNA Transport / Q000187:drug effects / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism; D003313:Zea mays / Q000187:drug effects / Q000378:metabolism
chemical_list: D017302:Annexins; D018076:DNA, Complementary; D007477:Ions; D010937:Plant Growth Regulators; D010940:Plant Proteins; D012333:RNA, Messenger; D002118:Calcium
keywords: Annexin; cotton (Gossypium hirsutum); fiber development; gene expression