title: [Role of ARID3A in E2F target gene expression and cell growth].
abstract: ARID3A is a member of the AT-rich interaction domain (ARID) family of DNA-binding proteins. ARID3A was isolated as proteins binding to E2F1, and stimulates transcription mediated by the E2F transcription factor that plays a central role in regulating cell cycle progression. However, the function of ARID3A in E2F-target-gene expression has not been fully understood.
  
  
  
  Gene-silencing and overexpression experiments were carried out using siRNA and recombinant adenoviruses, respectively. E2F responsive gene expression was measured by RT-PCR. Effects of ARID3A silencing on DNA synthesis and cell growth were determined by EdU incorporation and colony formation assay, respectively.
  
  
  
  siRNA mediated gene silencing of ARID3A blocked the transcription of E2F-target genes, such as E2F1, p107, CDC2 and CDC6 in normal human dermal fibroblasts (NHDFs). Although adenoviral-mediated overexpression of ARID3A did not up-regulate the transcription of these E2F-target genes in quiescent NHDFs, E2F1 overexpression was unable to overcome the blockade of CDC6 expression by ARID3A silencing. Furthermore, ARID3A silencing attenuated S phase entry of NHDFs, and suppressed growth of human tumor cell lines.
  
  
  
  These results indicate that ARID3A plays an important role for E2F-mediated transcriptional activation and cell growth.
authors: Section of Molecular Craniofacial Embryology, Department of Maxillofacial and Neck Reconstruction, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University.
authors: Khandakar A S M
authors: KA
authors: Saadat
mesh_terms: D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000502:physiology*; D050684:E2F Transcription Factors / Q000502:physiology*; D015870:Gene Expression; D020868:Gene Silencing; D006801:Humans; D014157:Transcription Factors / Q000502:physiology*; D014158:Transcription, Genetic / Q000502:physiology
chemical_list: C490798:ARID3A protein, human; D004268:DNA-Binding Proteins; D050684:E2F Transcription Factors; D014157:Transcription Factors