title: Effects of water-holding capability of the PVF sponge on the adhesion and differentiation of rat bone marrow stem cell culture.
abstract: The aim of the study is to estimate the effects of the water-holding capability of the polyvinyl formal (PVF) sponges on osteogenic response in vitro experiments. The rat bone marrow stem cells (BMCs) were seeded and cultured for up to 4 weeks under static conditions in osteogenic media to evaluate the adhesion, proliferation, differentiation, and mineralization on the Dextran-coated PVF sponges with or without water-holding capability. The BMCs seeded onto the PVF sponges with water-holding capability showed more significant increases in DNA content, alkaline phosphatase (ALP) activity, osteocalcin content, and calcium deposition than those without water-holding capability. These results suggest that the Dextran-coated PVF sponges with high water-holding capability would have potential uses as both a new scaffold to bone tissue engineering and as a new biomaterial.
authors: Department of Orthopaedic Surgery, Faculty of Life Sciences, Kumamoto University, 1-1-1 Honjo Chuo-ku,, Kumamoto-city, 860-8556, Japan.
authors: Wakana
authors: W
authors: Togami
authors: Akira
authors: A
authors: Sei
authors: Tatsuya
authors: T
authors: Okada
authors: Takuya
authors: T
authors: Taniwaki
authors: Toru
authors: T
authors: Fujimoto
authors: Takayuki
authors: T
authors: Nakamura
authors: Shogo
authors: S
authors: Tahata
authors: Yoshitaka
authors: Y
authors: Nakanishi
authors: Hiroshi
authors: H
authors: Mizuta
mesh_terms: D000818:Animals; D000943:Antigens, Differentiation / Q000096:biosynthesis; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003911:Dextrans / Q000737:chemistry*; D008297:Male; D010012:Osteogenesis; D051381:Rats; D011916:Rats, Inbred F344; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D014867:Water / Q000737:chemistry*
chemical_list: D000943:Antigens, Differentiation; D020099:Coated Materials, Biocompatible; D003911:Dextrans; D014867:Water
keywords: bone marrow stem cells; coated polyvinyl formal sponge; high water‐holding capability; osteogenic ability