title: Simultaneous determination by LC-MS/MS of 25-methoxydammarane-3β,12β,20-triol epimers and active metabolites in rat plasma after intravenous administration.
abstract: 1. The pharmacokinetics of the 25-OCH3-PPD epimers and active metabolites in rat plasma after a single intravenous (i.v.) administration were studied by a rapid, selective and sensitive UPLC-MS/MS method. 2. Chromatographic separation was performed on an Acquity UPLC with Agela C18 column, and the solvents of 5 mM ammonium acetate (pH 7.8) - acetonitrile (65: 35, v/v) were used as mobile phase for elution. The quantification was performed with the transitions of m/z 493.5 → 475.5 for 20(R,S)-25-OCH3-PPD, m/z 479.5 → 461.5 for 20(R,S)-25-OH-PPD. The Lower Limit Of Quantitation (LLOQ) was 20.0 ng mL(-1) for 20(R,S)-25-OCH3-PPD, 2.0 ng mL(-1) for 20(R,S)-25-OH-PPD in the plasma samples assay. 3. The pharmacokinetic parameters of AUC, t1/2 and MRT had no difference between 20(R)- and (S)-25-OCH3-PPD, but S-epimer has a lower plasma clearance compared to the R-isomer. The active metabolite 20(S)-25-OH-PPD showed significantly higher AUC, MRT and a longer half-life than that of 20(R)-25-OH-PPD. These assay results are necessary for the evaluation of the pharmacokinetic behavior of 25-methoxydammarane-3β,12β,20-triol in vivo.
authors: Department of Traditional Chinese Material Medica and.
authors: Cai-Hong
authors: CH
authors: Shi
authors: Xiangrong
authors: X
authors: Zhang
authors: Hao
authors: H
authors: Suo
authors: Tian
authors: T
authors: Yin
authors: Haiyan
authors: H
authors: Xu
authors: Bo
authors: B
authors: Yuan
authors: Yuqing
authors: Y
authors: Zhao
mesh_terms: D061605:Administration, Intravenous; D000818:Animals; D019540:Area Under Curve; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D005260:Female; D006207:Half-Life; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014315:Triterpenes / Q000008:administration & dosage* / Q000097:blood* / Q000378:metabolism / Q000493:pharmacokinetics; D000097669:Dammaranes
chemical_list: D014315:Triterpenes; C517965:25-methoxydammarane-3,12,20-triol; D000097669:Dammaranes