title: Effects of stress on AMPA receptor distribution and function in the basolateral amygdala.
abstract: Stress is a growing public health concern and can lead to significant disabilities. The neural response to stressors is thought to be dependent on the extended amygdala. The basolateral amygdala (BLA) is responsible for associations of sensory stimuli with emotional valence and is thought to be involved in stress-induced responses. Previous behavioral and electrophysiological experiments demonstrate that, in response to stress, changes occur in glutamatergic neurotransmission within the BLA and, in particular in transmission at AMPA receptors. Given the established role of AMPA receptors in memory and synaptic plasticity, we tested the hypothesis that stress produces alterations in the distribution of these receptors in a way that might account for stress-induced alterations in amygdala circuitry function. We examined the subcellular localization of GluR1 subunits of the AMPA receptor and the electrophysiological characteristics of BLA principal neurons in an animal model of unpredictable stress. Compared to controls, we demonstrated an increase in the ratio of labeled spines to labeled dendritic shafts in the BLA of rats 6 and 14 days post-stress, but not 1 day post-stress. Furthermore, the frequency of mini-EPSCs was increased in stressed animals without a change in general membrane properties, mini-EPSC amplitude, or in paired pulse modulation of glutamate release. Taken together, these data suggest that the shift of GluR1-containing AMPA receptors from dendritic stores into spines may be in part responsible for the persistent behavioral alterations observed following severe stressors.
authors: Department of Psychiatry, Atlanta Veteran's Medical Center, Atlanta, GA, USA, whubert50@gmail.com.
authors: G W
authors: GW
authors: Hubert
authors: C
authors: C
authors: Li
authors: D G
authors: DG
authors: Rainnie
authors: E C
authors: EC
authors: Muly
mesh_terms: D000818:Animals; D066272:Basolateral Nuclear Complex / Q000378:metabolism*; D003712:Dendrites / Q000378:metabolism; D049229:Dendritic Spines / Q000378:metabolism; D004597:Electroshock; D019706:Excitatory Postsynaptic Potentials; D017774:Long-Term Potentiation / Q000502:physiology*; D008297:Male; D055369:Miniature Postsynaptic Potentials / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D018091:Receptors, AMPA / Q000378:metabolism*; D013312:Stress, Physiological / Q000502:physiology*; D013315:Stress, Psychological / Q000378:metabolism* / Q000503:physiopathology
chemical_list: D018091:Receptors, AMPA
references: Mol Psychiatry. 2001 Jan;6(1):13-34
references: 11244481
references: J Neurophysiol. 1993 Apr;69(4):1350-62
references: 8492168
references: Neuropharmacology. 2012 Jun;62(7):2430-9
references: 22387532
references: Biol Psychiatry. 2006 Aug 15;60(4):376-82
references: 16919525
references: Pharmacol Biochem Behav. 2002 Mar;71(3):379-92
references: 11830172
references: Ann N Y Acad Sci. 1997 Jun 21;821:83-98
references: 9238196
references: Eur J Neurosci. 2007 May;25(10):3109-14
references: 17561824
references: PLoS One. 2011;6(10):e26065
references: 22022509
references: Front Psychiatry. 2011 Nov 14;2:62
references: 22102841
references: Nat Rev Neurosci. 2008 Nov;9(11):813-25
references: 18854855
references: J Neurochem. 2008 Jul;106(2):889-99
references: 18466342
references: Hippocampus. 2012 Jan;22(1):82-91
references: 20848608
references: J Comp Neurol. 1995 Nov 6;362(1):86-108
references: 8576430
references: J Neurosci. 2008 Nov 26;28(48):12682-90
references: 19036961
references: Proc Natl Acad Sci U S A. 2005 Jun 28;102(26):9371-6
references: 15967994
references: Int Rev Neurobiol. 2010;91:205-33
references: 20813244
references: Ann N Y Acad Sci. 2011 Jan;1216:114-21
references: 21272015
references: J Neurosci. 2004 Apr 7;24(14):3471-9
references: 15071094
references: Brain Struct Funct. 2018 May;223(4):1731-1745
references: 29204911
references: J Neurophysiol. 2007 Dec;98(6):3185-96
references: 17898152
references: Neuron. 2009 Nov 12;64(3):381-90
references: 19914186
references: Hippocampus. 2002;12(5):592-9
references: 12440575
references: Neuroscience. 2011 Oct 27;194:282-90
references: 21723920
references: Neurobiol Learn Mem. 2001 Jan;75(1):10-29
references: 11124044
references: Ann N Y Acad Sci. 1997 Jun 21;821:305-31
references: 9238214
references: Br Med Bull. 2012;101:127-45
references: 22334281
references: Curr Opin Neurobiol. 2002 Apr;12(2):205-10
references: 12015238
references: Neuropharmacology. 2007 Jan;52(1):215-27
references: 16919687
references: Learn Mem. 2009 Oct 28;16(11):687-97
references: 19864295
references: Physiol Rev. 2003 Jul;83(3):803-34
references: 12843409
references: Psychopharmacol Bull. 2007;40(1):8-30
references: 17285093
references: J Comp Neurol. 1991 Mar 15;305(3):370-92
references: 1709953
references: Neurobiol Learn Mem. 2010 Feb;93(2):165-74
references: 19766728
references: Biol Mood Anxiety Disord. 2011 Nov 01;1(1):8
references: 22738227
references: J Neurosci. 2007 Oct 10;27(41):11112-21
references: 17928453
references: J Comp Neurol. 2003 Dec 22;467(4):521-35
references: 14624486
references: Science. 2005 Apr 1;308(5718):83-8
references: 15746389
references: J Physiol. 1972 Nov;226(3):825-46
references: 4637631
references: J Neuroendocrinol. 2009 Mar;21(4):415-20
references: 19187468
references: Nature. 1997 Dec 11;390(6660):604-7
references: 9403688
references: Brain Res. 2005 Feb 21;1035(1):32-40
references: 15713274
references: Neuroscience. 2006 Dec 1;143(2):387-93
references: 16962717
references: Neuroscience. 2010 Jul 14;168(3):670-9
references: 20416363
references: J Neurosci. 2007 Apr 18;27(16):4482-91
references: 17442833
references: Neuroimage. 2003 Jul;19(3):587-600
references: 12880790
references: Neuron. 1999 Jun;23(2):229-32
references: 10399930
references: Behav Neurosci. 1994 Feb;108(1):210-2
references: 7910746
references: Prog Neurobiol. 1998 Jun;55(3):257-332
references: 9643556
references: Neuroscience. 2012 Dec 6;225:9-21
references: 22922122
references: J Neurosci. 2004 Apr 14;24(15):3810-5
references: 15084662
references: Am J Psychiatry. 2007 Oct;164(10):1476-88
references: 17898336
references: Eur Neuropsychopharmacol. 2003 Dec;13(6):435-41
references: 14636959
references: Neuropsychopharmacology. 2010 Jan;35(1):169-91
references: 19625997
references: Curr Top Med Chem. 2011;11(6):609-17
references: 21261589
references: J Comp Neurol. 2010 Dec 1;518(23):4723-39
references: 20963825
references: Am J Psychiatry. 2010 Jun;167(6):648-62
references: 20231322
references: J Biol Chem. 1992 Jan 5;267(1):501-7
references: 1309749
references: Proc Natl Acad Sci U S A. 2010 Aug 10;107(32):14449-54
references: 20663957
references: Stress. 2005 Dec;8(4):209-19
references: 16423710
references: Eur J Neurosci. 2004 Apr;19(7):1887-94
references: 15078562
references: Trends Neurosci. 1994 May;17(5):208-14
references: 7520203
references: J Neurophysiol. 1999 Jul;82(1):69-85
references: 10400936
references: Learn Mem. 2001 May-Jun;8(3):156-63
references: 11390635
references: Schizophr Bull. 1996;22(2):271-82
references: 8782286
references: Learn Mem. 2012 May 21;19(6):256-63
references: 22615481
references: Neuroscience. 2010 Sep 15;169(4):1557-66
references: 20600664
references: J Neurosci. 2002 Aug 1;22(15):6810-8
references: 12151561
references: J Neurosci. 2005 Sep 21;25(38):8725-34
references: 16177042
references: Brain Struct Funct. 2008 Sep;213(1-2):29-42
references: 18528706
references: Neuron. 1995 Jul;15(1):127-36
references: 7619517