title: Suppression effect of LHRH-A and hCG on Piwi expression in testis of Nile tilapia Oreochromis niloticus.
abstract: Piwi proteins as constituent factors of the piRNA pathway are required for germline maintenance, meiosis and gonad development. Previous study showed hCG could regulate the Piwi expression in ovary of teleosts. In this study, we revealed effects of LHRH-A and hCG on Piwi expression in testis of tilapia using Real-time PCR and Western blot. Both in vivo and in vitro study suggest that LHRH-A and hCG significantly down-regulated Piwil-1 and Piwil-2 in mRNA or protein levels compared with controls. Meanwhile, tissue and cell distribution showed that Piwi proteins were mainly expressed in spermatocytes rather than mature sperms. These results indicated that HPG suppresses Piwis which may play a crucial role in testis differentiation and development.
authors: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Animal Reproduction Institute, Guangxi University, Nanning, Guangxi 530004, China.
authors: Jun
authors: J
authors: Xiao
authors: Yi
authors: Y
authors: Zhou
authors: Yongju
authors: Y
authors: Luo
authors: Huan
authors: H
authors: Zhong
authors: Yulin
authors: Y
authors: Huang
authors: Yan
authors: Y
authors: Zhang
authors: Zujuan
authors: Z
authors: Luo
authors: Zhengbao
authors: Z
authors: Ling
authors: Ming
authors: M
authors: Zhang
authors: Xi
authors: X
authors: Gan
mesh_terms: D000818:Animals; D060565:Argonaute Proteins / Q000378:metabolism*; D006063:Chorionic Gonadotropin / Q000494:pharmacology*; D023681:Cichlids / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D006801:Humans; D008297:Male; D013737:Testis / Q000187:drug effects / Q000378:metabolism*
chemical_list: D060565:Argonaute Proteins; D006063:Chorionic Gonadotropin; D007987:Gonadotropin-Releasing Hormone