title: Short-term hypoxia/reoxygenation activates the angiogenic pathway in rat caudate putamen.
abstract: In response to hypoxia, tissues have to implement numerous mechanisms to enhance oxygen delivery, including the activation of angiogenesis. This work investigates the angiogenic response of the hypoxic caudate putamen after several recovery times. Adult Wistar rats were submitted to acute hypoxia and analysed after 0 h, 24 h and 5 days of reoxygenation. Expression of hypoxia-inducible factor-1 alfa (HIF-1 alpha) and angiogenesis-related genes including vascular endothelial growth factor (VEGF), adrenomedullin (ADM) and transforming growth factor-beta 1 (TGF- beta 1) was determined by both RT-PCR and ELISA. For vessel labelling, lectin location and expression were analysed using histochemical and image processing techniques (fractal dimension). Expression of Hif-1 alpha, Vegf, Adm and Tgf- beta 1 mRNA rose immediately after hypoxia and this increase persisted in some cases after 5 days post-hypoxia. While VEGF and TGF-beta 1 protein levels increased parallel to mRNA expression, ADM remained unaltered. The quantification of the striatal vessel network showed a significant augmentation at 24 h of reoxygenation. These results reveal that not only short-term hypoxia, but also the subsequent reoxygenation period, up-regulate the angiogenic pathway in the rat caudate putamen as a neuroprotective mechanism to hypoxia that seeks to maintain a proper blood supply to the hypoxic tissue, thereby minimizing the adverse effects of oxygen deprivation.
authors: Department of Health Science, University of Jaen, Paraje Las Lagunillas s/n 23071, Jaen, Spain.
authors: F
authors: F
authors: Molina
authors: A
authors: A
authors: Rus
authors: M A
authors: MA
authors: Peinado
authors: M L
authors: ML
authors: Del Moral
mesh_terms: D053607:Adrenomedullin / Q000235:genetics / Q000378:metabolism; D000818:Animals; D015687:Cell Hypoxia; D015870:Gene Expression; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism; D037102:Lectins / Q000378:metabolism; D008297:Male; D018919:Neovascularization, Physiologic; D011699:Putamen / Q000098:blood supply / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D015533:Transcriptional Activation; D053773:Transforming Growth Factor beta1 / Q000235:genetics / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: C497444:Hif1a protein, rat; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D037102:Lectins; C508962:Tgfb1 protein, rat; D053773:Transforming Growth Factor beta1; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat; D053607:Adrenomedullin
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