title: A survey of fermentation products and bacterial communities in corn silage produced in a bunker silo in China.
abstract: To evaluate the current practice of corn silage management in China, samples of bunker-made silage were collected from 14 farms within a 500-km radius of Beijing for the analysis of fermentation products and bacterial communities. Mean values for dry matter (DM) content were as low as 250 g/kg in both corn stover (St) and whole crop corn (Wc) silages, and pH values averaged 4.48 and 3.73, respectively. Only three of the 14 silages exhibited a lactic-to-acetic acid ratio > 1.0, indicating that the presence of acetic acid was predominant in fermentation. Although 1,2-propanediol content was marginal in most cases (< 5.0 g/kg dry matter (DM)), two Wc silages had 1,2-propanediol levels > 25 g/kg DM. In contrast, 3 St silages had large amounts (> 10 g/kg DM) of butyric acid, and two of the three butyrate silages also had high concentrations of 1-propanol. Denaturing gradient gel electrophoresis analysis demonstrated that the bacterial community appeared similar in 10 out of the 14 silage samples. Bands indicating Lactobacillus buchneri, L. acetotolerans and Acetobacter pasteurianus were found in both the St and Wc silages, accounting for the high acetic acid content found across silage samples.
authors: Department of Animal Science, Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan.
authors: Chao
authors: C
authors: Wang
authors: Hongyan
authors: H
authors: Han
authors: Xueying
authors: X
authors: Gu
authors: Zhu
authors: Z
authors: Yu
authors: Naoki
authors: N
authors: Nishino
mesh_terms: D000433:1-Propanol / Q000032:analysis; D019342:Acetic Acid / Q000032:analysis; D000091:Acetobacter / Q000302:isolation & purification*; D020148:Butyric Acid / Q000032:analysis; D002681:China; D058645:Denaturing Gradient Gel Electrophoresis; D005285:Fermentation; D019344:Lactic Acid / Q000032:analysis; D007778:Lactobacillus / Q000302:isolation & purification*; D019946:Propylene Glycol / Q000032:analysis; D012820:Silage / Q000032:analysis* / Q000382:microbiology*; D003313:Zea mays / Q000382:microbiology*
chemical_list: D020148:Butyric Acid; D019344:Lactic Acid; D019946:Propylene Glycol; D000433:1-Propanol; D019342:Acetic Acid
keywords: DGGE; bacteria; corn; silage