title: Tumor-related gene changes in immunosuppressive Syrian hamster cholangiocarcinoma.
abstract: The results of a previous study demonstrated that prednisolone enhanced cholangiocarcinogenesis. Therefore, to clarify molecular changes during immunosuppressive cholangiocarcinogenesis, Syrian hamsters were divided into 8 groups: uninfected controls; immunosuppressed Syrian hamsters using prednisolone (P); normal Syrian hamsters administered N-nitrosodimethylamine (ND); immunosuppressed Syrian hamsters administered N-nitrosodimethylamine (NDis); normal Syrian hamsters infected with Opisthorchis viverrini (OV); immunosuppressed Syrian hamsters infected with O. viverrini (OVis); normal Syrian hamsters infected with O. viverrini and administered N-nitrosodimethylamine (CCA); and immunosuppressed Syrian hamsters infected with O. viverrini and administered N-nitrosodimethylamine (CCAis). Syrian hamster livers were used for analysis of tumor-related gene expression and immunohistochemistry through cytokeratin 19 (CK19) and proliferating cell nuclear antigen (PCNA) staining. The tumor-related gene expression results show that CCAis groups at all time points exhibited upregulation of COX-2, IL-6, SOD1, CAT and iNOS and downregulation of p53, which correlated with the predominant expression of CK19 and PCNA in liver tissue. These results suggest that prednisolone enhances cholangiocarcinoma development, which was confirmed by molecular changes.
authors: Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
authors: Amornrat
authors: A
authors: Juasook
authors: Ratchadawan
authors: R
authors: Aukkanimart
authors: Thidarut
authors: T
authors: Boonmars
authors: Pakkayanee
authors: P
authors: Sudsarn
authors: Nadchanan
authors: N
authors: Wonkchalee
authors: Porntip
authors: P
authors: Laummaunwai
authors: Pranee
authors: P
authors: Sriraj
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001650:Bile Duct Neoplasms / Q000235:genetics* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D001653:Bile Ducts, Intrahepatic / Q000473:pathology*; D002374:Catalase / Q000235:genetics / Q000378:metabolism; D018281:Cholangiocarcinoma / Q000235:genetics* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D006224:Cricetinae; D051546:Cyclooxygenase 2 / Q000235:genetics / Q000378:metabolism; D004128:Dimethylnitrosamine / Q000494:pharmacology; D015972:Gene Expression Regulation, Neoplastic; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D053539:Keratin-19 / Q000737:chemistry / Q000378:metabolism; D008647:Mesocricetus; D009889:Opisthorchiasis; D011239:Prednisolone / Q000494:pharmacology; D018809:Proliferating Cell Nuclear Antigen / Q000737:chemistry / Q000378:metabolism; D013482:Superoxide Dismutase / Q000235:genetics / Q000378:metabolism; D000072105:Superoxide Dismutase-1
chemical_list: D000893:Anti-Inflammatory Agents; D015850:Interleukin-6; D053539:Keratin-19; D018809:Proliferating Cell Nuclear Antigen; D011239:Prednisolone; D002374:Catalase; D051546:Cyclooxygenase 2; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D004128:Dimethylnitrosamine
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