title: Dry eye modifies the thermal and menthol responses in rat corneal primary afferent cool cells.
abstract: Dry eye syndrome is a painful condition caused by inadequate or altered tear film on the ocular surface. Primary afferent cool cells innervating the cornea regulate the ocular fluid status by increasing reflex tearing in response to evaporative cooling and hyperosmicity. It has been proposed that activation of corneal cool cells via a transient receptor potential melastatin 8 (TRPM8) channel agonist may represent a potential therapeutic intervention to treat dry eye. This study examined the effect of dry eye on the response properties of corneal cool cells and the ability of the TRPM8 agonist menthol to modify these properties. A unilateral dry eye condition was created in rats by removing the left lacrimal gland. Lacrimal gland removal reduced tears in the dry eye to 35% compared with the contralateral eye and increased the number of spontaneous blinks in the dry eye by over 300%. Extracellular single-unit recordings were performed 8-10 wk following surgery in the trigeminal ganglion of dry eye animals and age-matched controls. Responses of corneal cool cells to cooling were examined after the application of menthol (10 μM-1.0 mM) to the ocular surface. The peak frequency of discharge to cooling was higher and the cooling threshold was warmer in dry eye animals compared with controls. The dry condition also altered the neuronal sensitivity to menthol, causing desensitization to cold-evoked responses at concentrations that produced facilitation in control animals. The menthol-induced desensitization of corneal cool cells would likely result in reduced tearing, a deleterious effect in individuals with dry eye.
authors: Division of Oral Physiology, Department of Oral Biological Sciences, Niigata University, Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors: Masayuki
authors: M
authors: Kurose
authors: Ian D
authors: ID
authors: Meng
mesh_terms: D000818:Animals; D003315:Cornea / Q000166:cytology / Q000502:physiology*; D008297:Male; D008610:Menthol / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D050053:TRPM Cation Channels / Q000819:agonists; D013696:Temperature; D013823:Thermoreceptors / Q000502:physiology; D012668:Trigeminal Ganglion / Q000503:physiopathology; D014985:Xerophthalmia / Q000503:physiopathology*
chemical_list: D050053:TRPM Cation Channels; C498947:Trpm8 protein, rat; D008610:Menthol
keywords: cold cells; dry eye; rat; sensitization; trigeminal
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