title: High-content imaging and analysis of pluripotent stem cell-derived cardiomyocytes.
abstract: Human pluripotent stem cells (hPSC) are investigated as a source of authentic human cardiac cells for drug discovery and toxicological tests. Cell-based assays using automated fluorescence imaging platform and high-content analysis characterize hypertrophic and toxicity profiles of compounds in hPSC-derived cardiomyocytes (hPSC-CM) at the cellular and subcellular levels. In purified population of hPSC-CM loaded with cell tracer probe and cell death markers, both hypertrophic and toxicity profiles can be assessed in live cardiomyocyte cultures. Alternatively, in non-purified cultures of hPSC-CM, hypertrophy, proliferation, and cell death assays can be performed specifically in the cardiomyocyte subpopulation using antibodies directed against cardiac proteins and a combination of cell death- and proliferation-specific fluorescent probes.
authors: Imperial Centre for Experimental and Translational Medicine, National Heart and Lung Institute, Imperial College London, London, UK.
authors: Gábor
authors: G
authors: Földes
authors: Maxime
authors: M
authors: Mioulane
mesh_terms: D017209:Apoptosis; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D003952:Diagnostic Imaging / Q000379:methods*; D006801:Humans; D006984:Hypertrophy; D032383:Myocytes, Cardiac / Q000378:metabolism*; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: D015415:Biomarkers