title: High-throughput sequencing of B- and T-lymphocyte antigen receptors in hematology.
abstract: Application of high-throughput DNA sequencing to the analysis of B- and T-lymphocyte antigen receptors has great potential for improving the monitoring of lymphoid malignancies, assessing immune reconstitution after hematopoietic cell transplantation, and characterizing the composition of lymphocyte repertoires. Current technology can define the number and frequency of immunoglobulin heavy, T-cell receptor (TCR)α, TCRβ, or TCRγ chains expressed in a population of lymphocytes; techniques for determining the number of antigen receptor heterodimers, such as TCRαβ pairs, expressed in the population are under development.
authors: Program in Immunology, Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA. ehwarren@u.washington.edu
authors: Edus H
authors: EH
authors: Warren
authors: Frederick A
authors: FA
authors: Matsen
authors: Jeffrey
authors: J
authors: Chou
mesh_terms: D018360:Crystallography, X-Ray; D019281:Dimerization; D019337:Hematologic Neoplasms / Q000235:genetics / Q000473:pathology; D006405:Hematology / Q000295:instrumentation*; D018380:Hematopoietic Stem Cell Transplantation; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D009360:Neoplastic Cells, Circulating / Q000473:pathology; D017434:Protein Structure, Tertiary; D011947:Receptors, Antigen, B-Cell / Q000737:chemistry / Q000235:genetics*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000737:chemistry / Q000235:genetics*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000737:chemistry / Q000235:genetics*
chemical_list: D011947:Receptors, Antigen, B-Cell; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta
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