title: Association of IL-17, IL-21, and IL-23R gene polymorphisms with HBV infection in kidney transplant patients.
abstract: Inflammatory cytokine gene polymorphisms may influence the hepatic and extrahepatic HBV-related disease in transplant patients. In this study, the association between IL-17, IL-23R, and IL-21 gene polymorphisms with hepatitis B virus (HBV) infection was evaluated in kidney transplant patients. In total, 220 kidney transplant patients were enrolled in this cross-sectional study between years 2007 and 2011. The genomic HBV DNA was identified using an HBV PCR detection Kit according to the manufacturer's instruction. The cytokine gene polymorphisms, including IL-17 197 A/G (rs2275913), IL-21 +1472 G/T (rs2055979), IL-21 5250 C/T (rs4833837), and IL-23R C/A (rs10889677) were evaluated by PCR-RFLP and ARMS-PCR protocols. The serum levels of IL-17 and IL-21 were analyzed in HBV infected and noninfected transplant patients by ELISA methods according to manufacturer's instructions. 70 of 220 (35%) transplant patients experienced acute rejection. HBV DNA was detected in 52 of 220 (23.64%) transplant patients. 16 of 52 (30.8%) HBV-infected kidney transplant patients experienced acute rejection. A significant higher frequency of C allele of IL-23R (rs10889677) polymorphism, a higher frequency of AG heterozygote genotype and A allele of IL-17-G197A (rs2275913) polymorphism, a higher frequency of TT homozygote genotype and T allele of IL-21-G1472T (rs2055979) polymorphism, and a higher frequency of CC homozygote genotype and C allele of IL-21-C5250T (rs4833837) polymorphism were found in HBV-infected kidney transplant patients with acute rejection. Diagnosis of the higher frequency of the IL-17, IL-21, and IL-23R cytokine genotypes and allele polymorphisms in HBV-infected kidney transplant patients who experienced acute rejection, illustrates the importance of Th17-related cytokine genetic patterns. A better evaluation of HBV infection in kidney transplant patients is needed.
authors: Shiraz Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors: Sara
authors: S
authors: Hejr
authors: Mohammad Hossein
authors: MH
authors: Karimi
authors: Ramin
authors: R
authors: Yaghobi
authors: Eskandar
authors: E
authors: Kamali-Sarvestani
authors: Bita
authors: B
authors: Geramizadeh
authors: Jamshid
authors: J
authors: Roozbeh
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002675:Child, Preschool; D003430:Cross-Sectional Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005787:Gene Frequency; D006509:Hepatitis B / Q000235:genetics* / Q000276:immunology; D006801:Humans; D007223:Infant; D020381:Interleukin-17 / Q000097:blood / Q000235:genetics*; D007378:Interleukins / Q000097:blood / Q000235:genetics*; D016030:Kidney Transplantation; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D018123:Receptors, Interleukin / Q000235:genetics*; D014180:Transplantation; D055815:Young Adult; D000098546:Interleukin-21
chemical_list: C515500:IL23R protein, human; D020381:Interleukin-17; D007378:Interleukins; D018123:Receptors, Interleukin; D000098546:Interleukin-21