title: Fibronectin is essential for reparative cardiac progenitor cell response after myocardial infarction.
abstract: BACKGROUND
  Adoptive transfer of cardiac progenitor cells (CPCs) has entered clinical application, despite limited mechanistic understanding of the endogenous response after myocardial infarction (MI). Extracellular matrix undergoes dramatic changes after MI and therefore might be linked to CPC-mediated repair.
  
  
  OBJECTIVE
  To demonstrate the significance of fibronectin (Fn), a component of the extracellular matrix, for induction of the endogenous CPC response to MI.
  
  
  RESULTS
  This report shows that presence of CPCs correlates with the expression of Fn during cardiac development and after MI. In vivo, genetic conditional ablation of Fn blunts CPC response measured 7 days after MI through reduced proliferation and diminished survival. Attenuated vasculogenesis and cardiogenesis during recovery were evident at the end of a 12-week follow-up period. Impaired CPC-dependent reparative remodeling ultimately leads to continuous decline of cardiac function in Fn knockout animals. In vitro, Fn protects and induces proliferation of CPCs via β₁-integrin-focal adhesion kinase-signal transducer and activator of transcription 3-Pim1 independent of Akt.
  
  
  CONCLUSIONS
  Fn is essential for endogenous CPC expansion and repair required for stabilization of cardiac function after MI.
authors: San Diego State University Integrated Regenerative Research Institute, San Diego, CA 92182, USA.
authors: Mathias H
authors: MH
authors: Konstandin
authors: Haruhiro
authors: H
authors: Toko
authors: Grady M
authors: GM
authors: Gastelum
authors: Pearl
authors: P
authors: Quijada
authors: Andrea
authors: A
authors: De La Torre
authors: Mercedes
authors: M
authors: Quintana
authors: Brett
authors: B
authors: Collins
authors: Shabana
authors: S
authors: Din
authors: Daniele
authors: D
authors: Avitabile
authors: Mirko
authors: M
authors: Völkers
authors: Natalie
authors: N
authors: Gude
authors: Reinhard
authors: R
authors: Fässler
authors: Mark A
authors: MA
authors: Sussman
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D005353:Fibronectins / Q000502:physiology*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009203:Myocardial Infarction / Q000378:metabolism* / Q000473:pathology*; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: D005353:Fibronectins
keywords: adult stem cells; cell adhesion molecules fibronectins; myocardial infarction
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