title: Immobilization of BMP-2 on a nano-hydroxyapatite-coated titanium surface using a chitosan calcium chelating agent.
abstract: We conducted experiments to determine the most effective calcium chelating agents for use in enhancing adhesion of human bone marrow mesenchymal stem cells (BM-MSCs) on nano-hydroxyapatite (nHAp)-coated titanium substrates by covalently immobilizing bone morphogenetic protein-2 (BMP-2). The quantity of amine groups on the chitosan chelated surface was 7 µg/surface area, and it was 1.4 µg/surface area on the alendronate chelated surface. The quantity of BMP-2 on the BMP-2 immobilized surface chelated with chitosan (4 ng/surface area) was higher than that on BMP-2 immobilized surface chelated with alendronate (2.2 ng/surface area). Contact angles of the nHAp-coated titanium, alendronate chelated, chitosan chelated, and BMP-2 immobilized surfaces chelated with alendronate were 68.8 ± 3.6°, 78.2 ± 1.9°, 74.8 ± 5.2°, and 76.0 ± 2.5°, respectively. The contact angle of the BMP-2 immobilized surface chelated with chitosan was significantly lower (56.2 ± 2.0°) than that of any of the other groups. BM-MSCs on the chitosan surface and BMP-2 immobilized on the surface chelated with chitosan appeared to be healthy and showed a spindle-like fibroblastic morphology. In addition, BM-MSCs on these surfaces appeared to have the ability to differentiate into bone-forming cells. We suggest that chitosan can be used as an effective calcium chelating agent for implants.
authors: Department of Medical Biotechnology, Dongguk University, Seoul, Korea.
authors: Sung-Hyun
authors: SH
authors: Kim
authors: Jung-Keug
authors: JK
authors: Park
authors: Kug-Sun
authors: KS
authors: Hong
authors: Hyun-Suk
authors: HS
authors: Jung
authors: Young-Kwon
authors: YK
authors: Seo
mesh_terms: D019386:Alendronate / Q000737:chemistry / Q000494:pharmacology; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002614:Chelating Agents / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D048271:Chitosan / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology* / Q000633:toxicity; D020099:Coated Materials, Biocompatible; D015921:Dental Implants; D004337:Drug Carriers; D017886:Durapatite / Q000737:chemistry*; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D019169:Jurkat Cells; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000378:metabolism; D053758:Nanoparticles; D036103:Nanotechnology; D010012:Osteogenesis / Q000187:drug effects; D011474:Prosthesis Design; D013499:Surface Properties; D054457:Tissue Scaffolds; D014025:Titanium / Q000737:chemistry*
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D002614:Chelating Agents; D020099:Coated Materials, Biocompatible; D015921:Dental Implants; D004337:Drug Carriers; D048271:Chitosan; D017886:Durapatite; D014025:Titanium; D002118:Calcium; D019386:Alendronate