title: Genetic Diversity of Plasmodium falciparum Field Isolates in Central Sudan Inferred by PCR Genotyping of Merozoite Surface Protein 1 and 2.
abstract: BACKGROUND
  Characterization of Plasmodium falciparum diversity is commonly achieved by amplification of the polymorphic regions of the merozoite surface proteins 1 (MSP1) and 2 (MSP2) genes.
  
  
  OBJECTIVE
  The present study aimed to determine the allelic variants distribution of MSP1 and MSP2 and multiplicity of infection in P. falciparum field isolates from Kosti, central Sudan, an area characterized by seasonal malaria transmission.
  
  
  METHODS
  Total 121 samples (N = 121) were collected during a cross-sectional survey between March and April 2003. DNA was extracted and MSP1 and MSP2 polymorphic loci were genotyped.
  
  
  RESULTS
  The total number of alleles identified in MSP1 block 2 was 11, while 16 alleles were observed in MSP2 block 3. In MSP1, RO33 was found to be the predominant allelic type, carried alone or in combination with MAD20 and K1 types, whereas FC27 family was the most prevalent in MSP2. Sixty two percent of isolates had multiple genotypes and the overall mean multiplicity of infection was 1.93 (CI 95% 1.66-2.20). Age correlated with parasite density (P = 0.017). In addition, a positive correlation was observed between parasite densities and the number of alleles (P = 0.022).
  
  
  CONCLUSIONS
  Genetic diversity in P. falciparum field isolates in central Sudan was high and consisted of multiple clones.
authors: Department of Molecular Biology, Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan.
authors: Muzamil M Abdel
authors: MM
authors: Hamid
authors: Sara B
authors: SB
authors: Mohammed
authors: Ibrahim M
authors: IM
authors: El Hassan
keywords: Genetic diversity; Merozoite surface protein; Multiplicity of infection
references: Exp Parasitol. 1995 Aug;81(1):90-6
references: 7628572
references: Malar J. 2008 Jan 23;7:17
references: 18215251
references: Lancet. 2009 Oct 31;374(9700):1480-2
references: 19880004
references: Parasitol Res. 2007 Dec;102(1):29-34
references: 17768641
references: Infect Immun. 2006 Feb;74(2):1313-22
references: 16428781
references: Am J Trop Med Hyg. 2001 May-Jun;64(5-6):303-9
references: 11463122
references: J Mol Biol. 1987 May 20;195(2):273-87
references: 3079521
references: J Infect Dis. 2009 Oct 1;200(7):1166-75
references: 19702508
references: Malar J. 2009 Jun 20;8:135
references: 19545390
references: Trop Med Int Health. 2005 Oct;10(10):1060-4
references: 16185241
references: Malar J. 2007 Jul 28;6:97
references: 17662153
references: Trans R Soc Trop Med Hyg. 2000 Nov-Dec;94(6):645-51
references: 11198648
references: Acta Trop. 2008 Jul;107(1):37-42
references: 18501320
references: Trans R Soc Trop Med Hyg. 1999 Jul-Aug;93(4):369-74
references: 10674079
references: Mol Microbiol. 2004 Apr;52(1):159-68
references: 15049818
references: Trop Med Int Health. 2001 Aug;6(8):607-13
references: 11555426
references: N Am J Med Sci. 2011 Mar;3(3):137-41
references: 22540079
references: Acta Trop. 2002 Jul;83(1):71-82
references: 12062795
references: Malar J. 2011 Sep 22;10:276
references: 21936949
references: Mol Cell. 1999 Apr;3(4):457-64
references: 10230398
references: Am J Trop Med Hyg. 1997 Feb;56(2):141-7
references: 9080871
references: Gene. 1990 Jul 2;91(1):57-62
references: 2205540
references: Am J Trop Med Hyg. 1998 Nov;59(5):726-35
references: 9840589
references: Trop Med Int Health. 2006 May;11(5):613-9
references: 16640613
references: Malar J. 2011 Apr 06;10:79
references: 21470428
references: Proc Natl Acad Sci U S A. 1991 Mar 1;88(5):1751-5
references: 2000383
references: Parasitology. 2000 Feb;120 ( Pt 2):105-11
references: 10726272
references: Sci Prog. 2009;92(Pt 1):1-38
references: 19544698
references: Trans R Soc Trop Med Hyg. 1991 Sep-Oct;85(5):572-7
references: 1780978
references: Mol Biochem Parasitol. 1993 Apr;58(2):283-92
references: 8479452
references: PLoS One. 2010 Mar 19;5(3):e9608
references: 20333256
references: J Infect Dis. 2001 Jul 15;184(2):237-41
references: 11424024
references: Mem Inst Oswaldo Cruz. 1992;87 Suppl 3:37-42
references: 1343716
references: Mol Biochem Parasitol. 1991 Dec;49(2):265-75
references: 1775170