title: Regulation of asymmetric cell division and polarity by Scribble is not required for humoral immunity.
abstract: The production of protective antibody requires effective signalling of naive B cells following encounter with antigen, and the divergence of responding B lymphocytes into distinct lineages. Polarity proteins have recently been proposed as important mediators of both the initial B cell response, and potentially of asymmetric cell division. Here we show that, although polarity proteins of the Scribble complex, Scribble, Dlg1 and Lgl1, are expressed and polarized during early B cell activation, their deficiency has no effect on the in vivo outcome of immunization or challenge with influenza infection. Furthermore, we find a striking correlation in the differentiation outcome of daughters of single founder B cells in vitro. Taken together, our results indicate that B cell differentiation does not require polarity proteins of the Scribble complex, and the findings do not support a role for asymmetric cell division in B cell activation and differentiation.
authors: Cancer Immunology Program, Peter MacCallum Cancer Centre, Melbourne, Victoria 3002, Australia.
authors: Edwin D
authors: ED
authors: Hawkins
authors: Jane
authors: J
authors: Oliaro
authors: Axel
authors: A
authors: Kallies
authors: Gabrielle T
authors: GT
authors: Belz
authors: Andrew
authors: A
authors: Filby
authors: Thea
authors: T
authors: Hogan
authors: Nicole
authors: N
authors: Haynes
authors: Kelly M
authors: KM
authors: Ramsbottom
authors: Vanessa
authors: V
authors: Van Ham
authors: Tanja
authors: T
authors: Kinwell
authors: Benedict
authors: B
authors: Seddon
authors: Derek
authors: D
authors: Davies
authors: David
authors: D
authors: Tarlinton
authors: Andrew M
authors: AM
authors: Lew
authors: Patrick O
authors: PO
authors: Humbert
authors: Sarah M
authors: SM
authors: Russell
mesh_terms: D000818:Animals; D000917:Antibody Formation / Q000187:drug effects / Q000276:immunology; D000956:Antigens, Viral / Q000276:immunology; D060049:Asymmetric Cell Division / Q000187:drug effects / Q000276:immunology*; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D019070:Cell Lineage / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D016764:Cell Polarity / Q000187:drug effects / Q000276:immunology*; D002469:Cell Separation; D005434:Flow Cytometry; D006410:Hematopoiesis / Q000187:drug effects / Q000276:immunology; D056724:Immunity, Humoral / Q000187:drug effects / Q000276:immunology*; D047908:Intracellular Signaling Peptides and Proteins / Q000172:deficiency / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D013154:Spleen / Q000166:cytology
chemical_list: D000956:Antigens, Viral; D047908:Intracellular Signaling Peptides and Proteins; D008070:Lipopolysaccharides; C508433:scrib protein, mouse
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