title: Antiangiogenetic effects of anthranoids from Alternaria sp., an endophytic fungus in a Thai medicinal plant Erythrina variegata.
abstract: Endophytic fungi are known as a prolific source for the discovery of structurally interesting and biologically active secondary metabolites, some of which are promising candidates for drug development. In the present study, three anthranoids were isolated from an Alternaria sp. endophytic fungus and evaluated for their antiangiogenic activity in a rat aortic sprouting assay, an ex vivo model of angiogenesis. Of these three compounds, altersolanol (2) was further characterized and found to show a promising activity in ex vivo, in vitro and in vivo angiogenesis asssays. Using human umbilical vein endothelial cells as an in vitro model, the angiogenic effect of 2 was found to occur via suppression of all three main functions of endothelial cells, namely proliferation, tube formation and migration.
authors: Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
authors: Phunlap
authors: P
authors: Pompeng
authors: Damrong
authors: D
authors: Sommit
authors: Nongluksna
authors: N
authors: Sriubolmas
authors: Nattaya
authors: N
authors: Ngamrojanavanich
authors: Kiminori
authors: K
authors: Matsubara
authors: Khanitha
authors: K
authors: Pudhom
mesh_terms: D000528:Alternaria / Q000737:chemistry* / Q000302:isolation & purification; D020533:Angiogenesis Inhibitors / Q000302:isolation & purification*; D000818:Animals; D000880:Anthraquinones / Q000302:isolation & purification* / Q000494:pharmacology; D060026:Endophytes / Q000737:chemistry* / Q000302:isolation & purification; D004895:Erythrina / Q000382:microbiology*; D061307:Human Umbilical Vein Endothelial Cells; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018919:Neovascularization, Physiologic / Q000187:drug effects; D010946:Plants, Medicinal / Q000382:microbiology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D020533:Angiogenesis Inhibitors; D000880:Anthraquinones; C058810:macrosporin; C058812:altersolanol A
keywords: Alternaria species; Anthranoid; Antiangiogenesis; Endophytic fungus; Erythrina variegata