title: [Distribution of deltamethrin in acute poisoned rats].
abstract: OBJECTIVE
  To establish an animal model in acute poisoned rat by deltamethrin and an analysis method for determination of deltamethrin by gas chromatography-electron capture detector (GC-ECD) and to study the distribution of deltamethrin in rats in order to provide the references for forensic medicine identification about such cases.
  
  
  METHODS
  Rats were administered with deltamethrin of different doses(512 and 1,024 mg/kg) and killed 1.5 h later to be dissected rapidly for tissues (blood, hearts, livers, lungs, kidneys and brains etc.). Samples were dehydrated by anhydrous sodium sulfate and extracted with petroleum ether and acetone (V:V=4:1). The level of deltamethrin was determined by GC-ECD.
  
  
  RESULTS
  There was a good separate between deltamethrin and endogenous impurities. The limit of quantification for deltamethrin in blood and liver were 0.1 microg/mL and 0.1 microg/g (S/N> or =10), respectively. The recovery rate of deltamethrin in blood was 91.55%-134.37% and both inter-day and intra-day precisions were less than 5.67%. The distribution of deltamethrin in poisoned rats with 512 mg/kg was as follow: lungs > livers > hearts > kidneys > blood > brains and with 1 024 mg/kg dose was lungs > blood > hearts > kidneys > brains > livers (P<0.05).
  
  
  CONCLUSIONS
  The GC-ECD method is sensitive for determination of deltamethrin. The distribution of deltamethrin in rats has a dose-dependent manner. The study suggests that samples of blood, hearts, livers, lungs, kidneys and brains are suitable for deltamethrin poisoned analysis.
authors: College of Forensic Medicine, Shanxi Medical University, Taiyuan, China. luoyejingyou@163.com
authors: Bin
authors: B
authors: Wu
authors: Peng
authors: P
authors: Yan
authors: Zhi-Wen
authors: ZW
authors: Wei
authors: Yu-Jin
authors: YJ
authors: Wang
mesh_terms: D000818:Animals; D002849:Chromatography, Gas / Q000295:instrumentation / Q000379:methods*; D004195:Disease Models, Animal; D053593:Forensic Toxicology / Q000379:methods; D007668:Kidney / Q000378:metabolism; D016014:Linear Models; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism*; D008297:Male; D009570:Nitriles / Q000097:blood / Q000493:pharmacokinetics* / Q000506:poisoning*; D011722:Pyrethrins / Q000097:blood / Q000493:pharmacokinetics* / Q000506:poisoning*; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014018:Tissue Distribution
chemical_list: D009570:Nitriles; D011722:Pyrethrins; C017180:decamethrin