title: Through gap junction communications, co-cultured mast cells and fibroblasts generate fibroblast activities allied with hypertrophic scarring.
abstract: BACKGROUND
  The prominent inflammatory cell identified in excessive scarring is the mast cell. Hypertrophic scar exhibits myofibroblasts derived from the transformation of fibroblasts, increased collagen synthesis, and stationary nonmigratory resident cells. The co-culture of fibroblasts with an established rat mast cell line (RMC-1) was used to explore the hypothesis of whether mast cells through gap junctional intercellular communications guide fibroblasts in promoting excessive scarring.
  
  
  METHODS
  Human dermal fibroblasts were cultured alone or co-cultured with RMC-1 cells as is or with either blocked gap junctional intercellular communications or devoid of cytoplasmic granules. Collagen synthesis was analyzed by dot blot analysis; immunohistology identified myofibroblasts, and a cell migration assay measured fibroblast locomotion.
  
  
  RESULTS
  Fibroblasts co-cultured with RMC-1 cells transformed into myofibroblasts, had increased collagen synthesis, and showed retarded cell migration. In contrast, RMC-1 cells unable to form gap junctional intercellular communications were similar to fibroblasts alone, failing to promote these activities. Degranulated RMC-1 cells were as effective as intact RMC-1 cells.
  
  
  CONCLUSIONS
  Mast cells induce fibroblast activities associated with hypertrophic scarring through gap junctional intercellular communications. Eliminating the mast cell or its gap junctional intercellular communications with fibroblasts may be a possible approach in preventing hypertrophic scarring or reducing fibrotic conditions.
authors: Hershey, Pa. From the Division of Plastic Surgery, Department of Surgery, Pennsylvania State University, College of Medicine.
authors: Theodore T
authors: TT
authors: Foley
authors: H Paul
authors: HP
authors: Ehrlich
mesh_terms: D000818:Animals; D002450:Cell Communication / Q000502:physiology*; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology; D017439:Cicatrix, Hypertrophic / Q000473:pathology* / Q000503:physiopathology; D018920:Coculture Techniques; D003094:Collagen / Q000096:biosynthesis; D005347:Fibroblasts / Q000166:cytology* / Q000502:physiology; D005355:Fibrosis / Q000473:pathology / Q000503:physiopathology; D052816:Foreskin / Q000166:cytology; D017629:Gap Junctions / Q000502:physiology*; D006801:Humans; D008297:Male; D008407:Mast Cells / Q000166:cytology* / Q000502:physiology; D058628:Myofibroblasts / Q000166:cytology* / Q000502:physiology; D051381:Rats
chemical_list: D003094:Collagen
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