title: Structures of the phage Sf6 large terminase provide new insights into DNA translocation and cleavage.
abstract: Many DNA viruses use powerful molecular motors to cleave concatemeric viral DNA into genome-length units and package them into preformed procapsid powered by ATP hydrolysis. Here we report the structures of the DNA-packaging motor gp2 of bacteriophage Sf6, which reveal a unique clade of RecA-like ATPase domain and an RNase H-like nuclease domain tethered by a regulatory linker domain, exhibiting a strikingly distinct domain arrangement. The gp2 structures complexed with nucleotides reveal, at the atomic detail, the catalytic center embraced by the ATPase domain and the linker domain. The gp2 nuclease activity is modulated by the ATPase domain and is stimulated by ATP. An extended DNA-binding surface is formed by the linker domain and the nuclease domain. These results suggest a unique mechanism for translation of chemical reaction into physical motion of DNA and provide insights into coordination of DNA translocation and cleavage in a viral DNA-packaging motor, which may be achieved via linker-domain-mediated interdomain communication driven by ATP hydrolysis.
authors: Department of Molecular Biosciences, University of Kansas, Lawrence, KS 66045, USA.
authors: Haiyan
authors: H
authors: Zhao
authors: Theodore E
authors: TE
authors: Christensen
authors: Yvonne N
authors: YN
authors: Kamau
authors: Liang
authors: L
authors: Tang
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry; D000255:Adenosine Triphosphate / Q000737:chemistry; D001435:Bacteriophages / Q000201:enzymology*; D020134:Catalytic Domain; D042003:DNA Packaging; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics; D004706:Endodeoxyribonucleases / Q000737:chemistry*; D016679:Genome, Viral; D006868:Hydrolysis; D009711:Nucleotides / Q000737:chemistry; D011485:Protein Binding; D017434:Protein Structure, Tertiary
chemical_list: D004279:DNA, Viral; D009711:Nucleotides; D000255:Adenosine Triphosphate; D004706:Endodeoxyribonucleases; C030894:terminase; D000251:Adenosine Triphosphatases
keywords: genome packaging; terminase; virus assembly
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