title: Molecular characteristics of meningiomas in a cohort of Indian patients: loss of heterozygosity analysis of chromosomes 22, 17, 14 and 10.
abstract: BACKGROUND
  Though, loss of heterozygosity (LOH) at chromosome 22q is considered to be the most likely initiating event in the formation of meningiomas, LOH at other chromosomes (1, 3, 6, 9, 10, 11, 14.17, and 18) have been implicated in its progression. The aim of this study was to analyze microsatellite markers on a select set of chromosomes including, 22q, 10q, 14q, and 17p for LOH in patients with meningiomas.
  
  
  METHODS
  Tumor tissue and its corresponding blood sample were collected from 27 patients with meningioma. Four polymorphic microsatellite markers (D10S520, D17S1289, D14S555, and D22S417) were characterized for LOH analysis.
  
  
  RESULTS
  There were 14 World Health Organization (WHO) grade I, 12 WHO grade II and 1 WHO grade III meningiomas. LOH was seen most often at D22S417 with an equal distribution between the grades (33% of informative samples in each grade). Though, LOH at D14S555 was seen in 50% of informative WHO grade II tumors, compared to 11.1% of informative WHO grade I tumors it did not reach statistical significance. However, allelic imbalance (AI) at D14S555 was significantly associated with atypia (P = 0.05). LOH at D17S1289 was seen only in one tumor sample, and none of the informative samples displayed LOH at D10S520.
  
  
  CONCLUSIONS
  The frequency and equal distribution of LOH at chromosome 22 supports the hypothesis that it is an early event in the tumorigenesis of meningiomas. The association of AI at D14S555 in WHO grade II meningiomas needs to be investigated on a larger set of samples.
authors: Department of Pathology and Neurological Sciences, Christian Medical College, Vellore, Tamil Nadu, India.
authors: Jaya Ruth
authors: JR
authors: Asirvatham
authors: Rekha
authors: R
authors: Pai
authors: Geeta
authors: G
authors: Chacko
authors: Arun G
authors: AG
authors: Nehru
authors: Jacob
authors: J
authors: John
authors: Ari G
authors: AG
authors: Chacko
authors: Jayaprakash
authors: J
authors: Muliyil
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D002879:Chromosomes, Human, Pair 10; D002883:Chromosomes, Human, Pair 14; D002886:Chromosomes, Human, Pair 17; D002892:Chromosomes, Human, Pair 22; D005260:Female; D006801:Humans; D007194:India; D019656:Loss of Heterozygosity; D008297:Male; D008577:Meningeal Neoplasms / Q000235:genetics* / Q000473:pathology; D008579:Meningioma / Q000235:genetics* / Q000473:pathology; D018895:Microsatellite Repeats; D008875:Middle Aged