title: Optical induction of muscle contraction at the tissue scale through intrinsic cellular amplifiers.
abstract: The smooth muscle cell is the principal component responsible for involuntary control of visceral organs, including vascular tonicity, secretion, and sphincter regulation. It is known that the neurotransmitters released from nerve endings increase the intracellular Ca(2+) level in smooth muscle cells followed by muscle contraction. We herein report that femtosecond laser pulses focused on the diffraction-limited volume can induce intracellular Ca(2+) increases in the irradiated smooth muscle cell without neurotransmitters, and locally increased intracellular Ca(2+) levels are amplified by calcium-induced calcium-releasing mechanisms through the ryanodine receptor, a Ca(2+) channel of the endoplasmic reticulum. The laser-induced Ca(2+) increases propagate to adjacent cells through gap junctions. Thus, ultrashort-pulsed lasers can induce smooth muscle contraction by controlling Ca(2+), even with optical stimulation of the diffraction-limited volume. This optical method, which leads to reversible and reproducible muscle contraction, can be used in research into muscle dynamics, neuromuscular disease treatment, and nanorobot control.
authors: Department of Bio and Brain Engineering, KAIST, Daejeon, Korea; KAIST Institute for Optical Science and Technology, KAIST, Daejeon, Korea.
authors: Jonghee
authors: J
authors: Yoon
authors: Myunghwan
authors: M
authors: Choi
authors: Taeyun
authors: T
authors: Ku
authors: Won Jong
authors: WJ
authors: Choi
authors: Chulhee
authors: C
authors: Choi
mesh_terms: D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling; D005110:Extracellular Space / Q000378:metabolism; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D007834:Lasers; D009119:Muscle Contraction; D032389:Myocytes, Smooth Muscle / Q000166:cytology* / Q000378:metabolism; D055620:Optical Phenomena; D013997:Time Factors; D001743:Urinary Bladder / Q000166:cytology / Q000502:physiology
chemical_list: D002118:Calcium
keywords: Biophotonics; femtosecond-pulsed lasers; laser-tissue interaction; low-density plasma; muscle contraction