title: Effects of bone morphogenetic protein-6 on periodontal wound healing/regeneration in supraalveolar periodontal defects in dogs.
abstract: OBJECTIVE
  Application of a synthetic BMP-6 polypeptide in a rat periodontal fenestration defect model enhanced periodontal wound healing/regeneration including new bone and cementum formation. The purpose of this study was to translate the relevance of these initial observations into a discriminating large animal model.
  
  
  METHODS
  Critical-size (4-5 mm) supraalveolar periodontal defects were created at the 2(nd) and 3(rd) mandibular premolar teeth in 11 Beagle dogs. Experimental sites received BMP-6 at 0.25, 1.0 and 2.0 mg/ml soak-loaded onto an absorbable collagen sponge (ACS) carrier or ACS alone (control) each condition repeated in four jaw quadrants. The animals were euthanized at 8 weeks when block biopsies were collected and processed for histologic/histometric analysis.
  
  
  RESULTS
  BMP-6 at 0.25, 1.0 and 2.0 mg/ml soak-loaded onto the ACS yielded significantly enhanced new bone (0.99 ± 0.07 versus 0.23 ± 0.13 mm/BMP-6 at 0.25 mg/ml) and cementum (2.45 ± 0.54 versus 0.73 ± 0.15 mm/BMP-6 at 0.25 mg/ml) formation including a functionally oriented periodontal ligament compared with control (p < 0.05). A significant inverse linear association between BMP-6 dose and new bone (β = -0.21 ± 0.09 mm, p = 0.016) and cementum height (β = -0.34 ± 0.15 mm, p = 0.023) was observed. Minimal root resorption was observed without significant differences between groups. Ankylosis was not observed for any of the experimental groups.
  
  
  CONCLUSIONS
  Surgical application of BMP-6/ACS onto critical-size supraalveolar defects enhanced periodontal wound healing/regeneration, in particular cementogenesis including a functionally oriented periodontal ligament; the low BMP-6 0.25 mg/ml concentration apparently providing the most effective dose.
authors: Department of Periodontology, School of Dentistry, National Defense Medical Center and Tri-Service General Hospital, Taipei, Taiwan, ROC.
authors: Hsien-Chung
authors: HC
authors: Chiu
authors: Cheng-Yang
authors: CY
authors: Chiang
authors: Hsiao-Pei
authors: HP
authors: Tu
authors: Ulf M E
authors: UM
authors: Wikesjö
authors: Cristiano
authors: C
authors: Susin
authors: Earl
authors: E
authors: Fu
mesh_terms: D000818:Animals; D055418:Bone Morphogenetic Protein 6 / Q000494:pharmacology / Q000627:therapeutic use*; D001861:Bone Regeneration / Q000187:drug effects*; D025021:Cementogenesis / Q000187:drug effects*; D004285:Dogs; D005260:Female; D023421:Models, Animal; D010510:Periodontal Diseases / Q000188:drug therapy*; D010513:Periodontal Ligament / Q000502:physiology; D011994:Recombinant Proteins; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D055418:Bone Morphogenetic Protein 6; D011994:Recombinant Proteins