title: Neuroglobin expression in neurogenesis.
abstract: Neuroglobin is a hypoxia-inducible, neuroprotective protein related to myoglobin and hemoglobin, but little is known about its neurodevelopmental expression or function. To begin to explore these issues, we measured neuroglobin protein expression during neuronal differentiation of human embryonic stem cells in vitro and in the neurogenic subventricular zone of adult rats in vivo. Neuroglobin protein expression was barely detectable by western blotting in human embryonic stem cells, but was readily demonstrable in neural stem cells, and was further induced upon differentiation to neurons. In the adult subventricular zone, neuroglobin expression coincided with that of the neuronal lineage marker doublecortin, but not with vimentin or glial fibrillary acidic protein. These findings suggest that neuroglobin is expressed early in the course of neuronal differentiation and may, therefore, have a role in neurodevelopment.
authors: Buck Institute for Research on Aging, 8001 Redwood Boulevard, Novato, CA 94945, USA.
authors: Bryan
authors: B
authors: Haines
authors: Xiaoou
authors: X
authors: Mao
authors: Lin
authors: L
authors: Xie
authors: Steve
authors: S
authors: Spusta
authors: Xianmin
authors: X
authors: Zeng
authors: Kunlin
authors: K
authors: Jin
authors: David A
authors: DA
authors: Greenberg
mesh_terms: D000818:Animals; D002552:Cerebral Ventricles / Q000166:cytology / Q000378:metabolism; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D005914:Globins / Q000378:metabolism*; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D058953:Neural Stem Cells / Q000166:cytology / Q000378:metabolism*; D055495:Neurogenesis / Q000502:physiology*; D000078787:Neuroglobin; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism; D051381:Rats; D014746:Vimentin / Q000378:metabolism
chemical_list: C000714328:DCX protein, human; C000714848:Dcx protein, rat; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D005904:Glial Fibrillary Acidic Protein; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; D000078787:Neuroglobin; D009479:Neuropeptides; D014746:Vimentin; D005914:Globins
keywords: Dcx; GFAP; NSC; Neurogenesis; Neuroglobin; Ngb; SVZ; Stem cell; Subventricular zone; doublecortin; glial fibrillary acidic protein; hESC; human embryonic stem cells; neural stem cell; neuroglobin; subventricular zone