title: Dynamic metabolic profiling of cyanobacterial glycogen biosynthesis under conditions of nitrate depletion.
abstract: Cyanobacteria represent a globally important biomass because they are responsible for a substantial proportion of primary production in the hydrosphere. Arthrospira platensis is a fast-growing halophilic cyanobacterium capable of accumulating glycogen and has the potential to serve as a feedstock in the fermentative production of third-generation biofuels. Accordingly, enhancing cyanobacterial glycogen production is a promising biofuel production strategy. However, the regulatory mechanism of glycogen metabolism in cyanobacteria is poorly understood. The aim of the present study was to determine the metabolic flux of glycogen biosynthesis using a dynamic metabolomic approach. Time-course profiling of widely targeted cyanobacterial metabolic intermediates demonstrated a global metabolic reprogramming that involves transient increases in the levels of some amino acids during the glycogen production phase induced by nitrate depletion. Also, in vivo labelling with NaH(13)CO3 enabled direct measurement of metabolic intermediate turnover in A. platensis, revealing that under conditions of nitrate depletion glycogen is biosynthesized with carbon derived from amino acids released from proteins via gluconeogenesis. This dynamic metabolic profiling approach provided conclusive evidence of temporal alterations in the metabolic profile in cyanobacterial cells.
authors: Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657-8501, Japan. hasunuma@port.kobe-u.ac.jp
authors: Tomohisa
authors: T
authors: Hasunuma
authors: Fumi
authors: F
authors: Kikuyama
authors: Mami
authors: M
authors: Matsuda
authors: Shimpei
authors: S
authors: Aikawa
authors: Yoshihiro
authors: Y
authors: Izumi
authors: Akihiko
authors: A
authors: Kondo
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D056804:Biofuels; D000458:Cyanobacteria / Q000378:metabolism*; D005943:Gluconeogenesis; D006003:Glycogen / Q000096:biosynthesis*; D055442:Metabolome; D009566:Nitrates / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D056804:Biofuels; D009566:Nitrates; D006003:Glycogen
keywords: 13C; cyanobacteria; glycogen; in vivo labelling; metabolic turnover; metabolomics.
references: Appl Environ Microbiol. 2008 May;74(9):2787-96
references: 18310411
references: DNA Res. 1996 Jun 30;3(3):109-36
references: 8905231
references: Nature. 1968 Nov 9;220(5167):606-7
references: 4879742
references: Biochim Biophys Acta. 2012 Aug;1817(8):1483-9
references: 22285745
references: J Proteomics. 2011 Dec 10;75(1):284-305
references: 21802534
references: Plant J. 2008 Sep;55(5):857-68
references: 18494855
references: Bioresour Technol. 2011 Jan;102(1):186-93
references: 20663661
references: J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Oct 1;877(27):2952-60
references: 19631594
references: Microb Cell Fact. 2011 Jan 10;10(1):2
references: 21219616
references: Appl Microbiol Biotechnol. 2011 Aug;91(3):471-90
references: 21691792
references: BMC Genomics. 2011 Mar 14;12:148
references: 21401935
references: Curr Opin Biotechnol. 2008 Jun;19(3):235-40
references: 18539450
references: Biotechnol Bioeng. 2008 Jun 1;100(2):203-12
references: 18431744
references: Phytochemistry. 2007 Aug-Sep;68(16-18):2302-12
references: 17524438
references: Plant Physiol. 2008 Dec;148(4):2109-20
references: 18945936
references: Microbiol Rev. 1993 Sep;57(3):725-49
references: 8246846
references: Curr Opin Microbiol. 2009 Oct;12(5):547-52
references: 19695948
references: Curr Opin Microbiol. 2008 Jun;11(3):233-9
references: 18538626
references: Annu Rev Plant Biol. 2003;54:207-33
references: 14502990
references: J Biol Chem. 2006 Feb 3;281(5):2668-75
references: 16303755
references: Plant Physiol. 2005 Dec;139(4):1995-2005
references: 16306140
references: J Biol Chem. 2011 Sep 2;286(35):30962-30971
references: 21757761
references: Anal Bioanal Chem. 2011 Apr;399(10):3503-17
references: 21340691
references: Bioresour Technol. 2012 Mar;108:211-5
references: 22277210
references: J Biol Chem. 2004 Aug 27;279(35):36514-8
references: 15218021
references: Photosynth Res. 2005;83(2):117-33
references: 16143847
references: J Biosci Bioeng. 2012 May;113(5):665-73
references: 22280965
references: J Exp Bot. 2012 Mar;63(6):2293-308
references: 22371075
references: Anal Chem. 2000 Mar 15;72(6):1236-41
references: 10740865
references: Biotechnol Adv. 2010 Nov-Dec;28(6):817-30
references: 20630488
references: Anal Chem. 2011 May 15;83(10):3808-16
references: 21466217
references: Bioresour Technol. 2013 May;135:191-8
references: 23116819
references: J Exp Bot. 2010 Feb;61(4):1041-51
references: 20026474
references: Biotechnol Adv. 2012 Nov-Dec;30(6):1207-18
references: 22085593
references: Microbiol Mol Biol Rev. 1999 Mar;63(1):106-27
references: 10066832
references: Plant J. 2000 Nov;24(4):551-8
references: 11115136
references: Int J Biol Macromol. 2007 Apr 10;40(5):498-504
references: 17175020