title: Novel role for cystic fibrosis transmembrane conductance regulator in alveolar fluid clearance in lipopolysaccharide-induced acute lung injury in mice.
abstract: OBJECTIVE
  Alveolar fluid clearance (AFC) is important for the resolution of acute lung injury (ALI). The role of cystic fibrosis transmembrane conductance regulator (CFTR) in AFC has not been entirely elucidated in animal models of ALI. The aim of this study was to investigate the role of CFTR and its mechanisms in AFC in normal and ALI mice.
  
  
  METHODS
  Seventy mice were randomly divided into 14 groups and ALI was established by intratracheal instillation of lipopolysaccharide (LPS). After 48 h, CFTR activator CFTRact-16 or inhibitor CFinh-172 with or without β-agonist was instillated intratracheally and AFC was measured with radioisotopic tracer.
  
  
  RESULTS
  Although there was no effects of CFTRact-16 on AFC in mice with or without isoproterenol, CFinh-172 markedly decreased isoproterenol-stimulated AFC in both normal (P < 0.01) and LPS-induced ALI mice (P < 0.01) and there was significantly decreased basal AFC in ALI mice (P < 0.05).
  
  
  CONCLUSIONS
  These results provide direct functional evidence for CFTR in cAMP-mediated AFC in both normal and ALI mice.
authors: Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.
authors: Weizhong
authors: W
authors: Jin
authors: Yuanlin
authors: Y
authors: Song
authors: Chunxue
authors: C
authors: Bai
authors: Jinjun
authors: J
authors: Jiang
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced* / Q000503:physiopathology*; D000818:Animals; D000242:Cyclic AMP / Q000502:physiology; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000819:agonists / Q000037:antagonists & inhibitors / Q000502:physiology*; D004195:Disease Models, Animal; D007545:Isoproterenol / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000009:adverse effects*; D051379:Mice; D008815:Mice, Inbred Strains; D011650:Pulmonary Alveoli / Q000187:drug effects / Q000502:physiology*; D011654:Pulmonary Edema / Q000503:physiopathology
chemical_list: D008070:Lipopolysaccharides; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D000242:Cyclic AMP; D007545:Isoproterenol
keywords: acute lung injury; alveolar fluid clearance; cAMP; cystic fibrosis transmembrane conductance regulator