title: Chromosomal aberrations and deoxyribonucleic acid single-strand breaks in adipose-derived stem cells during long-term expansion in vitro.
abstract: OBJECTIVE
  Adipose-derived stem cells (ASCs) are a promising mesenchymal cell source for tissue engineering approaches. To obtain an adequate cell amount, in vitro expansion of the cells may be required in some cases. To monitor potential contraindications for therapeutic applications in humans, DNA strand breaks and chromosomal aberrations in ASCs during in vitro expansion were examined.
  
  
  METHODS
  After isolation of ASC from human lipoaspirates of seven patients, in vitro expansion over 10 passages was performed. Cells from passages 1, 2, 3, 5 and 10 were used for the alkaline single-cell microgel electrophoresis (comet) assay to detect DNA single-strand breaks and alkali labile as well as incomplete excision repair sites. Chromosomal changes were examined by means of the chromosomal aberration test.
  
  
  RESULTS
  During in vitro expansion, ASC showed no DNA single-strand breaks in the comet assay. With the chromosomal aberration test, however, a significant increase in chromosomal aberrations were detected.
  
  
  CONCLUSIONS
  The study showed that although no DNA fragmentation could be determined, the safety of ASC cannot be ensured with respect to chromosome stability during in vitro expansion. Thus, reliable analyses for detecting ASC populations, which accumulate chromosomal aberrations or even undergo malignant transformation during extensive in vitro expansion, must be implemented as part of the safety evaluation of these cells for stem cell-based therapy.
authors: Department of Otorhinolaryngology, Plastic, Aesthetic and Reconstructive Head and Neck Surgery, University of Wuerzburg, Germany. froelich_k@klinik.uni-wuerzburg.de
authors: Katrin
authors: K
authors: Froelich
authors: Johannes
authors: J
authors: Mickler
authors: Gudrun
authors: G
authors: Steusloff
authors: Antje
authors: A
authors: Technau
authors: Mario
authors: M
authors: Ramos Tirado
authors: Agmal
authors: A
authors: Scherzed
authors: Stephan
authors: S
authors: Hackenberg
authors: Andreas
authors: A
authors: Radeloff
authors: Rudolf
authors: R
authors: Hagen
authors: Norbert
authors: N
authors: Kleinsasser
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000235:genetics; D019070:Cell Lineage; D002470:Cell Survival / Q000235:genetics; D020219:Chondrogenesis / Q000235:genetics; D002869:Chromosome Aberrations; D002875:Chromosomes / Q000235:genetics; D053904:DNA Breaks, Single-Stranded; D004260:DNA Repair / Q000235:genetics; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*