title: Detection of eight periodontal microorganisms and distribution of Porphyromonas gingivalis fimA genotypes in Chinese patients with aggressive periodontitis.
abstract: BACKGROUND
  The microbiologic feature of aggressive periodontitis (AgP) in Chinese patients has not yet been determined. This study aims to investigate the prevalence of eight periodontal microorganisms and the distribution of the Porphyromonas gingivalis fimA genotype in a cohort of Chinese patients with AgP.
  
  
  METHODS
  Saliva and pooled subgingival plaque samples were collected from 81 patients with AgP (25 with incisor-first molar type and 56 with generalized type [GAgP]) and 34 periodontally healthy controls. Eight periodontal microorganisms, including Aggregatibacter actinomycetemcomitans, P. gingivalis, Tannerella forsythia, Treponema denticola, Campylobacter rectus, Prevotella intermedia, Prevotella nigrescens, and Fusobacterium nucleatum were detected in these samples by the polymerase chain reaction (PCR). In addition, the distribution of fimA genotypes was assessed in P. gingivalis-positive individuals by PCR.
  
  
  RESULTS
  The prevalence of P. gingivalis, T. forsythia, T. denticola, C. rectus, P. intermedia, F. nucleatum, and A. actinomycetemcomitans in patients with AgP was significantly higher than that in healthy controls. The prevalence of A. actinomycetemcomitans in patients with GAgP was relatively low (30.4%) compared with other pathogens. Results of logistic regression analysis showed that younger patients were more likely to harbor A. actinomycetemcomitans (odds ratio = 2.85). Type II was the most prevalent fimA genotype of P. gingivalis in patients with AgP.
  
  
  CONCLUSIONS
  P. gingivalis, T. forsythia, T. denticola, C. rectus, P. intermedia, and F. nucleatum were the predominant periodontal pathogens of patients with GAgP in China. Type II of fimA was the most prevalent genotype of P. gingivalis in patients with AgP. The prevalence of A. actinomycetemcomitans in patients with GAgP was relatively low.
authors: Department of Periodontology, Peking University School and Hospital of Stomatology, Haidian District, Beijing, China.
authors: Xianghui
authors: X
authors: Feng
authors: Li
authors: L
authors: Zhang
authors: Li
authors: L
authors: Xu
authors: Huanxin
authors: H
authors: Meng
authors: Ruifang
authors: R
authors: Lu
authors: Zhibin
authors: Z
authors: Chen
authors: Dong
authors: D
authors: Shi
authors: Xiane
authors: X
authors: Wang
mesh_terms: D000328:Adult; D000367:Age Factors; D016976:Aggregatibacter actinomycetemcomitans / Q000302:isolation & purification; D010520:Aggressive Periodontitis / Q000145:classification / Q000382:microbiology*; D001419:Bacteria / Q000145:classification*; D058491:Bacterial Load; D001439:Bacteroides / Q000302:isolation & purification; D044883:Campylobacter rectus / Q000302:isolation & purification; D015331:Cohort Studies; D003773:Dental Plaque / Q000382:microbiology; D005260:Female; D036781:Fimbriae Proteins / Q000235:genetics*; D016967:Fusobacterium nucleatum / Q000302:isolation & purification; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D018933:Pili, Sex / Q000235:genetics*; D016966:Porphyromonas gingivalis / Q000145:classification*; D018713:Prevotella intermedia / Q000302:isolation & purification; D045242:Prevotella nigrescens / Q000302:isolation & purification; D012463:Saliva / Q000382:microbiology; D048373:Treponema denticola / Q000302:isolation & purification; D055815:Young Adult
chemical_list: C063055:fimbrillin; D036781:Fimbriae Proteins