title: Interspecific bacterial sensing through airborne signals modulates locomotion and drug resistance.
abstract: Bacteria use chemical signals to sense each other and to regulate various physiological functions. Although it is known that some airborne volatile organic compounds function as bacterial signalling molecules, their identities and effects on global gene expression and bacterial physiological processes remain largely unknown. Here we perform microarray analyses of Escherichia coli exposed to volatile organic compounds emitted from Bacillus subtilis. We find that 2,3-butanedione and glyoxylic acid mediate global changes in gene expression related to motility and antibiotic resistance. Volatile organic compound-dependent phenotypes are conserved among bacteria and are regulated by the previously uncharacterized ypdB gene product through the downstream transcription factors soxS, rpoS or yjhU. These results strongly suggest that bacteria use airborne volatile organic compounds to sense other bacteria and to change master regulatory gene activity to adapt.
authors: Superbacteria Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Korea.
authors: Kwang-sun
authors: KS
authors: Kim
authors: Soohyun
authors: S
authors: Lee
authors: Choong-Min
authors: CM
authors: Ryu
mesh_terms: D000667:Ampicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001412:Bacillus subtilis / Q000378:metabolism*; D024881:Drug Resistance, Bacterial / Q000187:drug effects* / Q000235:genetics; D004352:Drug Resistance, Microbial / Q000187:drug effects / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005798:Genes, Bacterial / Q000235:genetics; D007700:Kinetics; D008124:Locomotion / Q000187:drug effects* / Q000235:genetics; D010641:Phenotype; D011401:Promoter Regions, Genetic / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects* / Q000235:genetics; D013045:Species Specificity; D055549:Volatile Organic Compounds / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; D055549:Volatile Organic Compounds; D000667:Ampicillin
references: Nature. 2008 Oct 9;455(7214):804-7
references: 18724361
references: J Bacteriol. 2009 Jun;191(11):3758-9
references: 19329631
references: Nucleic Acids Res. 2012 Jan;40(Database issue):D109-14
references: 22080510
references: Bacteriol Rev. 1972 Dec;36(4):525-57
references: 4568763
references: Science. 1998 Apr 10;280(5361):295-8
references: 9535661
references: Molecules. 2012 Jun 06;17(6):6982-95
references: 22728370
references: Annu Rev Microbiol. 2012;66:125-52
references: 22726217
references: Biotechnol Appl Biochem. 2007 Mar;46(Pt 3):169-78
references: 17115975
references: Nature. 2011 May 12;473(7346):174-80
references: 21508958
references: Cell. 2007 Sep 7;130(5):797-810
references: 17803904
references: Appl Environ Microbiol. 2005 Jul;71(7):4022-34
references: 16000817
references: Microbiology (Reading). 2007 Aug;153(Pt 8):2613-2620
references: 17660425
references: Mol Microbiol. 2011 Aug;81(3):705-16
references: 21651627
references: RNA Biol. 2012 Sep;9(9):1167-76
references: 22951591
references: Annu Rev Genet. 2010;44:71-90
references: 21047256
references: Mol Syst Biol. 2006;2:2006.0008
references: 16738554
references: Toxicol Sci. 2008 May;103(1):169-80
references: 18227102
references: Nucleic Acids Res. 2013 Jan;41(Database issue):D605-12
references: 23143106
references: Infect Immun. 2011 Aug;79(8):3149-58
references: 21576340
references: Nat Med. 2004 Dec;10(12 Suppl):S122-9
references: 15577930
references: Phytochemistry. 2006 Oct;67(20):2262-8
references: 16949113
references: FEMS Microbiol Rev. 2010 Jul;34(4):426-44
references: 20070374
references: Microbiology (Reading). 2007 Dec;153(Pt 12):3923-3938
references: 18048907
references: Can J Microbiol. 2012 Mar;58(3):326-35
references: 22339347
references: Microbiology (Reading). 2004 Dec;150(Pt 12):4023-31
references: 15583155
references: Nat Genet. 2000 May;25(1):25-9
references: 10802651
references: PLoS One. 2012;7(5):e36357
references: 22563494
references: Toxicol Appl Pharmacol. 2006 Aug 15;215(1):17-22
references: 16545411
references: Biotechniques. 2003 Feb;34(2):374-8
references: 12613259
references: PLoS Pathog. 2011 Aug;7(8):e1002192
references: 21829370
references: Mol Microbiol. 2003 Nov;50(4):1199-213
references: 14622409
references: Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6640-5
references: 10829079
references: Appl Environ Microbiol. 1982 Sep;44(3):525-32
references: 7137998
references: Biochem J. 1966 Apr;99(1):1-11
references: 5337756
references: J Bacteriol. 1983 Aug;155(2):768-75
references: 6348026
references: Trends Microbiol. 2000 Mar;8(3):103-4
references: 10707059
references: Appl Microbiol Biotechnol. 2009 Jan;81(6):1001-12
references: 19020812
references: Nature. 1993 Apr 1;362(6419):448-50
references: 8464476
references: Proc Natl Acad Sci U S A. 2003 Apr 15;100(8):4927-32
references: 12684534
references: Am J Bot. 1947 Jun;34(6):345-8
references: 20252518
references: Annu Rev Genet. 2009;43:197-222
references: 19686078
references: Mol Cell Biochem. 2010 Sep;342(1-2):207-13
references: 20480211
references: Science. 2009 Jan 16;323(5912):396-401
references: 19150849
references: J Mol Microbiol Biotechnol. 2012;22(4):215-27
references: 22964521
references: Biotechnol J. 2010 Sep;5(9):974-7
references: 20721987
references: Genome Res. 2001 Jul;11(7):1246-55
references: 11435407
references: Genome Biol. 2012 May 25;13(5):R37
references: 22632713
references: Nat Rev Drug Discov. 2003 Feb;2(2):114-22
references: 12563302
references: J Bacteriol. 2004 Dec;186(24):8172-80
references: 15576765
references: Appl Microbiol Biotechnol. 2006 Sep;72(2):361-7
references: 16397770
references: Proc Natl Acad Sci U S A. 2012 Dec 26;109(52):21390-5
references: 23236140
references: Plant Physiol. 2004 Mar;134(3):1017-26
references: 14976231
references: J Bacteriol. 2010 Nov;192(22):6103-4
references: 20851896
references: Clin Infect Dis. 2008 Oct 15;47(8):1070-6
references: 18781869
references: Nat Methods. 2006 Aug;3(8):623-8
references: 16862137
references: J Bacteriol. 2006 Jun;188(11):3826-36
references: 16707675
references: Nucleic Acids Res. 2011 Jan;39(Database issue):D596-600
references: 20929876
references: J Appl Microbiol. 2002;92(6):1116-22
references: 12010552
references: Toxicol Pathol. 2008 Feb;36(2):330-44
references: 18474946
references: BMC Microbiol. 2006 Jun 12;6:53
references: 16768798
references: Proc Natl Acad Sci U S A. 1999 Apr 13;96(8):4610-4
references: 10200310
references: Curr Top Microbiol Immunol. 2008;322:107-31
references: 18453274
references: Nat Rev Microbiol. 2008 Feb;6(2):111-20
references: 18197168
references: Mol Microbiol. 2006 Jul;61(2):297-309
references: 16856937