title: [The expression of bFGF, GAP-43 and neurogenesis after cerebral ischemia/reperfusion in rats].
abstract: OBJECTIVE
  To observe time points of the expressions of basic fibroblast growth factor (bFGF), growth associated protein-43 (GAP-43) and neurogenesis after cerebral ischemia/reperfusion in rats and explore its possible mechanism of neurogenesis.
  
  
  METHODS
  Models of middle cerebral artery occlusion (MCAO) were established in SD rats which were divided into 3 d, 7 d, 14 d and 28 d groups (n = 6). The neurological severity was evaluated by neurological severity scores (NSS) and scores of motor test (SMT). Neuronal injury in the boundary zone of the infarction area was evaluated by TUNEL and Nissl staining; The expressions of bFGF and GAP-43 and neurogenesis were evaluated by Western blot and 5-bromodeoxyuridine (Brdu) fluorescence staining, respectively.
  
  
  RESULTS
  It showed up neurologic impairment and motor dysfunction after cerebral ischemia/reperfusion in rats at 3 d, the numbers of neuron apoptosis also peaked at 3d, the protein levels of bFGF and GAP-43 were significantly increased in time-dependent manner, peaked at 7 d and then decreased gradually, meanwhile, Brdu and NeuN double fluorescence staining displayed scattered Brdu-and NeuN-positive cells in the boundary zone of the infarction area.
  
  
  CONCLUSIONS
  These results suggest that the upregulation of bFGF and GAP-43 may contribute to the neurogenesis after cerebral ischemia/reperfusion.
authors: The Affiliated Union Hospital of Fujian Medical University, Fuzhou 350001, China.
authors: Wang-Qing
authors: WQ
authors: Shi
authors: Guan-Yi
authors: GY
authors: Zheng
authors: Xiao-Dong
authors: XD
authors: Chen
authors: Yuan-Gui
authors: YG
authors: Zhu
authors: Jing
authors: J
authors: Zhang
authors: Qiong
authors: Q
authors: Jiang
mesh_terms: D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism / Q000503:physiopathology; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D019922:GAP-43 Protein / Q000378:metabolism*; D008297:Male; D055495:Neurogenesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000503:physiopathology
chemical_list: D019922:GAP-43 Protein; D016222:Fibroblast Growth Factor 2