title: [The genetic screening of a dominant zebrafish mutant in long-term memory].
abstract: OBJECTIVE
  To screen the learning and memory mutant from N-ethyl-N-nitrosourea (ENU) mutagenic zebrafish F1, and to get the new model animal to study the mechanism of learning and memory.
  
  
  METHODS
  Zebrafish mutant was screened by inhibitory avoidance behavioral test and identified by the expression of gene c-fos with qRT-PCR.
  
  
  RESULTS
  We isolated a zebrafish mutant related to learning and memory, fgt. In this fgt zebrafish mutant long-term memory was much lower than that in wild-type when tested at 24 h after training. The 24 h long-term memory in about half of fgt mutant F2 (13/30) were significantly lower than those in wild-type, and the others relatively normal. Compared with the expression in wild-type fishes, the expression of immediate-early genes (IEGs) c-fos in half of fgt mutant F2 (13/30) after exploring in a novel environment increased distinctly from the basal control levels statistically, and the others relatively normal, which were in accordance with the behavioral results.
  
  
  CONCLUSIONS
  The zebrafish mutant fgt is a dominant mutant with defect in long-term memory.
authors: Key Laboratory of Animal Models and Degenerative Neurological Diseases, Nankai University School of Medicine, Tianjin 300071, China.
authors: Xin-Le
authors: XL
authors: Li
authors: Lei
authors: L
authors: Sun
authors: Ning-Ning
authors: NN
authors: He
authors: Hua-Feng
authors: HF
authors: Zhang
authors: Dong-Yan
authors: DY
authors: Chen
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D017781:Genes, Immediate-Early; D005820:Genetic Testing; D008297:Male; D057567:Memory, Long-Term; D015027:Zebrafish / Q000235:genetics*