title: Molecular Characterization of Classical swine fever virus Involved in the Outbreak in Mizoram.
abstract: Classical swine fever is the most insidious and devastating disease of pigs and wild boars. The virus is closely related to the other members of the genus Pestivirus. The outbreak recorded in Mizoram, India was strategically important as the state shares porous international boundary with East Asian countries. Both immunodiagnostic and molecular techniques were used to confirm the involvement of Classical swine fever virus (CSFV) in this outbreak. Sandwich ELISA and direct FAT could detect CSFV in the tissue samples. RT-nPCR specifically amplified E2 and 5'NTR product of 271 bp. Phylogenetic analysis showed, that the Mizoram isolate (MZ4/69) was very close to the Chinese strain Shimen-HVRI (93.0%) rather than other Indian isolate (CSF-30-03). Present study provides a valuable sequence based molecular data on Indian isolate of CSFV and will be useful in investigation on transmission of such disease from neighbour countries.
authors: Department of Microbiology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, 781 022 Assam India.
authors: N N
authors: NN
authors: Barman
authors: R S
authors: RS
authors: Gupt
authors: D P
authors: DP
authors: Bora
authors: R S
authors: RS
authors: Kataria
authors: A K
authors: AK
authors: Tiwari
authors: P
authors: P
authors: Roychoudhury
keywords: Classical swine fever virus ELISA; RT-PCR; Sequence analysis
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