title: Electrochemical determination of microRNA-21 based on bio bar code and hemin/G-quadruplet DNAenzyme.
abstract: A simple and novel microRNA (miRNA) biosensor was developed using DNA-Au bio bar code (DNA-Au) and G-quadruplex-based DNAenzyme. DNA-Au increased the amount of miRNA-21 participating in hybridization. Hemin/G-quadruplex DNAenzyme significantly improved the catalysis of H(2)O(2) by oxidation of hydroquinone, resulting in an obvious reduction current of benzoquinone for miRNA-21 indirect detection. Under optimum conditions, the linear relationship between miRNA-21 concentration and reduction response was obtained with the detection limit of 0.006 pM, which showed a good sensitivity. Besides, selectivity of the biosensor was investigated by detecting the base mismatched miRNAs. This proposed method was further applied to detect miRNA-21 extracted from human hepatocarcinoma BEL-7402 cells and human mastocarcinoma MCF-7 cells. The influence of bisphenol A (BPA) on the expression of miRNA-21 in cells was also investigated. The biosensor performs well in practical applications, which suggests it may provide a new platform for gene diagnosis.
authors: College of Chemistry and Material Science, Shandong Agricultural University, 271018, Taian, Shandong, PR China.
authors: Xiaomeng
authors: X
authors: Meng
authors: Yunlei
authors: Y
authors: Zhou
authors: Qianjin
authors: Q
authors: Liang
authors: Xiangjin
authors: X
authors: Qu
authors: Qingqing
authors: Q
authors: Yang
authors: Huanshun
authors: H
authors: Yin
authors: Shiyun
authors: S
authors: Ai
mesh_terms: D001483:Base Sequence; D001559:Benzhydryl Compounds / Q000494:pharmacology; D015374:Biosensing Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D015342:DNA Probes / Q000737:chemistry / Q000235:genetics; D021881:DNA, Catalytic / Q000737:chemistry* / Q000378:metabolism*; D004563:Electrochemistry; D004566:Electrodes; D054856:G-Quadruplexes; D005786:Gene Expression Regulation / Q000187:drug effects; D006046:Gold / Q000737:chemistry; D006427:Hemin / Q000378:metabolism*; D006801:Humans; D035683:MicroRNAs / Q000032:analysis* / Q000737:chemistry / Q000235:genetics; D009693:Nucleic Acid Hybridization; D010636:Phenols / Q000494:pharmacology; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D015342:DNA Probes; D021881:DNA, Catalytic; D006046:Gold; D006427:Hemin; D035683:MicroRNAs; D010636:Phenols; C524940:MIRN21 microRNA, human; C006780:bisphenol A; D000098826:Bisphenol A Compounds