title: Study of zalema grape pomace: phenolic composition and biological effects in Caenorhabditis elegans.
abstract: The phenolic composition of the extractable fraction of Zalema grape pomace has been analyzed by HPLC-DAD-MS and consisted of mainly flavanols and flavonols (122.75 and 23.11 mg/100 g dry pomace, respectively). The antioxidant activity has been determined by in vitro FRAP, ABTS, and ORAC assays (11.7, 34.9, and 63.6 mmol of Trolox equivalents (TE) per 100 g of dry pomace, respectively) and in vivo using the model organism Caenorhabditis elegans . Cultivation of C. elegans in media containing 100 μg/mL dry pomace extract increased the survival of worms submitted to thermally induced oxidative stress, whereas a decrease in the rate of worm survival was found for 300 μg/mL extract. Interestingly, the levels of reactive oxygen species (ROS) were significantly decreased in stressed worms treated with the pomace extract at the two concentration levels. Further studies are required to explain this unexpected behavior, as well as to determine the compounds and mechanisms involved in the observed effects.
authors: Food Colour and Quality Laboratory, Department of Nutrition and Food Science, Facultad de Farmacia, Universidad de Sevilla, Sevilla, Spain.
authors: M José
authors: MJ
authors: Jara-Palacios
authors: Susana
authors: S
authors: González-Manzano
authors: M Luisa
authors: ML
authors: Escudero-Gilete
authors: Dolores
authors: D
authors: Hernanz
authors: Montserrat
authors: M
authors: Dueñas
authors: Ana M
authors: AM
authors: González-Paramás
authors: Francisco J
authors: FJ
authors: Heredia
authors: Celestino
authors: C
authors: Santos-Buelga
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010636:Phenols / Q000737:chemistry* / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D027843:Vitis / Q000737:chemistry*; D014866:Waste Products / Q000032:analysis
chemical_list: D010636:Phenols; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D014866:Waste Products