title: Ultrastructural and genomic characterization of a second banchine polydnavirus confirms the existence of shared features within this ichnovirus lineage.
abstract: Polydnaviruses (PDVs) are symbiotic viruses carried by endoparasitic wasps and transmitted to caterpillar hosts during parasitization. Although they share several features, including a segmented dsDNA genome, a unique life cycle where replication is restricted to the wasp host, and immunodepressive/developmental effects on the caterpillar host, PDVs carried by ichneumonid and braconid wasps (referred to as ichnoviruses and bracoviruses, respectively) have different evolutionary origins. In addition, ichnoviruses (IVs) form two distinct lineages, with viral entities found in wasps belonging to the subfamilies Campopleginae and Banchinae displaying strikingly different virion morphologies and genomic features. However, the current description for banchine IVs is based on the characterization of a single species, namely that of the Glypta fumiferanae IV (GfIV). Here we provide an ultrastructural and genomic analysis of a second banchine IV isolated from the wasp Apophua simplicipes, and we show that this virus shares many features with GfIV, including a multi-nucleocapsid virion, an aggregate genome size of ~300 kb, genome segments <5 kb, an impressively high degree of genome segmentation and a very similar gene content (same gene families in both viruses). Altogether, the data presented here confirm the existence of shared characteristics within this banchine IV lineage.
authors: Département de biochimie, de microbiologie et de bio-informatique, Université Laval, Québec, QC G1V 0A6, Canada.|Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du PEPS, PO Box 10380, Stn. Sainte-Foy, Québec, QC G1V 4C7, Canada.
authors: Abdelmadjid
authors: A
authors: Djoumad
authors: Department of Microbiology and Immunology, Sir Charles Tupper Medical Building, Dalhousie University, Halifax, NS B3H 4R2, Canada.
authors: Don
authors: D
authors: Stoltz
authors: Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du PEPS, PO Box 10380, Stn. Sainte-Foy, Québec, QC G1V 4C7, Canada.
authors: Catherine
authors: C
authors: Béliveau
authors: Institut de biologie intégrative et des systèmes, Université Laval, Québec, QC G1V 0A6, Canada.
authors: Brian
authors: B
authors: Boyle
authors: Department of Microbiology and Immunology, Sir Charles Tupper Medical Building, Dalhousie University, Halifax, NS B3H 4R2, Canada.
authors: Lisa
authors: L
authors: Kuhn
authors: Département de biochimie, de microbiologie et de bio-informatique, Université Laval, Québec, QC G1V 0A6, Canada.|Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, 1055 du PEPS, PO Box 10380, Stn. Sainte-Foy, Québec, QC G1V 4C7, Canada.
authors: Michel
authors: M
authors: Cusson
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D018071:Polydnaviridae / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure*; D017422:Sequence Analysis, DNA; D014771:Virion / Q000648:ultrastructure*; D014863:Wasps / Q000821:virology*
chemical_list: D004279:DNA, Viral