title: Quantitative imaging of subcellular metabolism with stable isotopes and multi-isotope imaging mass spectrometry.
abstract: Multi-isotope imaging mass spectrometry (MIMS) is the quantitative imaging of stable isotope labels in cells with a new type of secondary ion mass spectrometer (NanoSIMS). The power of the methodology is attributable to (i) the immense advantage of using non-toxic stable isotope labels, (ii) high resolution imaging that approaches the resolution of usual transmission electron microscopy and (iii) the precise quantification of label down to 1 part-per-million and spanning several orders of magnitude. Here we review the basic elements of MIMS and describe new applications of MIMS to the quantitative study of metabolic processes including protein and nucleic acid synthesis in model organisms ranging from microbes to humans.
authors: Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, 75 Francis Street, Boston, MA 02115, United States; Division of Genetics, Brigham and Women's Hospital, 75 Francis Street, Boston, MA 02115, United States; Division of Cardiovascular Medicine, Brigham and Women's Hospital, 75 Francis Street, Boston, MA 02115, United States.
authors: Matthew L
authors: ML
authors: Steinhauser
authors: Claude P
authors: CP
authors: Lechene
mesh_terms: D000818:Animals; D006801:Humans; D021621:Imaging, Three-Dimensional; D007553:Isotope Labeling / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D008660:Metabolism; D036103:Nanotechnology; D013347:Subcellular Fractions / Q000378:metabolism
keywords: Cell division; Human; Keywords: Stable isotope; Metabolism; Quantitative imaging; Stem cell
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