title: Hydrogen peroxide penetration into the pulp chamber and dental permeability after bleaching.
abstract: This study sought to quantify the concentration of hydrogen peroxide (HP) in the pulp chamber and evaluate changes on dental permeability after bleaching with 3 HP concentrations (10%, 35%, and 50%). This study was divided into 2 experiments and the bleaching treatments consisted of 3 applications of HP for 30 minutes during a single session. The first experiment tested HP penetration into the pulp chamber of 4 experimental groups (n = 10) of bovine crowns, which were divided by HP concentration: an unbleached control group (0% HP), 10% HP, 35% HP, and 50% HP. Acetate buffer solution was placed into the pulp chamber and after each application of HP. This solution was collected to determine spectrophotometrically the concentration of HP that reached the pulp chamber. The second experiment evaluated dental permeability. Bovine crowns were divided into 3 groups (n = 10). The crowns were connected to a permeability device and the initial permeability was measured at 10 psi. Three different concentrations of HP gels (10%, 35% and 50%) were applied to the buccal enamel surfaces and the dental permeability was measured after the first, second, and third applications of HP. The data were analyzed by 2-way ANOVA and Tukey test (P ≤ 0.05). All concentrations of HP reached the pulp chamber, although no significant differences were noted between the 3 concentrations tested (P > 0.05). However, the increase of dental permeability in the group that received 50% HP was significantly higher than the 10% HP group (P < 0.05). The results indicate that the HP bleaching treatments increased dental permeability.
authors: Department of Restorative Dentistry, School of Dentistry, University of North Parana, Londrina, Brazil.
authors: Sandrine Bittencourt
authors: SB
authors: Berger
authors: Cinthia Pereira Machado
authors: CP
authors: Tabchoury
authors: Glaucia Maria Bovi
authors: GM
authors: Ambrosano
authors: Marcelo
authors: M
authors: Giannini
mesh_terms: D000818:Animals; D002417:Cattle; D003743:Dental Enamel / Q000187:drug effects / Q000378:metabolism; D003745:Dental Enamel Permeability / Q000187:drug effects; D003786:Dental Pulp Cavity / Q000187:drug effects / Q000378:metabolism*; D003804:Dentin / Q000187:drug effects / Q000378:metabolism; D003806:Dentin Permeability / Q000187:drug effects; D005456:Fluorescent Dyes; D005840:Gentian Violet; D006861:Hydrogen Peroxide / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000494:pharmacology; D011897:Random Allocation; D013053:Spectrophotometry / Q000379:methods; D013997:Time Factors; D058205:Tooth Bleaching Agents / Q000008:administration & dosage / Q000493:pharmacokinetics* / Q000494:pharmacology; D019228:Tooth Crown / Q000187:drug effects / Q000378:metabolism; D014088:Tooth Permeability / Q000187:drug effects*
chemical_list: D005456:Fluorescent Dyes; D058205:Tooth Bleaching Agents; C475159:leucocrystal violet; D006861:Hydrogen Peroxide; D005840:Gentian Violet