title: Simple method to enhance the photostability of the fluorescence reporter R6G for prolonged single-molecule studies.
abstract: For fluorescence-based single-molecule studies, photobleaching of the dye reporter often limits the time window over which individual molecules can be followed. As such, many strategies, for example, using a cocktail of chemical reagents, have been developed to decrease the rate of photobleaching. Herein, we introduce a new and highly effective method to enhance the photostability of one of the commonly used fluorescent dyes, rhodamine 6G (R6G). We show that micrometer-sized polydimethylsiloxane (PDMS) wells, when the PDMS surface is properly treated, not only provide a confined environment for single-molecule detection but can also significantly increase the survival time of individual R6G molecules before photobleaching. Moreover, our results suggest, consistent with several previous studies, that R6G photobleaching involves a radical state.
authors: Department of Chemistry, University of Pennsylvania, 231 South 34th Street, Philadelphia, Pennsylvania 19104, USA.
authors: Lin
authors: L
authors: Guo
authors: Feng
authors: F
authors: Gai
mesh_terms: D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004355:Drug Stability; D005456:Fluorescent Dyes / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D055616:Microtechnology; D038761:Photobleaching; D012235:Rhodamines / Q000737:chemistry*; D013997:Time Factors; D053835:Unilamellar Liposomes / Q000737:chemistry; D014835:Volatilization
chemical_list: D004129:Dimethylpolysiloxanes; D005456:Fluorescent Dyes; D012235:Rhodamines; D053835:Unilamellar Liposomes; C026188:rhodamine 6G; C013830:baysilon
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