title: Comparison of the ultrastructural and immunophenotypic characteristics of human umbilical cord-derived mesenchymal stromal cells and in situ cells in Wharton's jelly.
abstract: The umbilical cord contains mucinous connective tissue, called Wharton's jelly. It consists of stromal cells, collagen fibers, and amorphous ground substances composed of proteoglycan. Recently, these stromal cells have been redefined as a new cell therapy source, named human umbilical cord-derived mesenchymal stromal cells (hUCMSCs). However, there are few studies on the ultrastructural features and immune-phenotypic characteristics of isolated hUCMSCs and comparisons with the cells found in original cord tissues. In this study, the authors describe and compare the phenotypic characteristics of hUCMSCs with cells in the umbilical cord in order to know the kinds of cells and ultrastructural changes. Isolated hUCMSCs showed similar ultrastructure with few structural differences from in situ stromal cells, and they are relatively homogenous and well-developed mesenchymal cells that demonstrate a myofibroblastic phenotype.
authors: Department of Pathology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.
authors: Young-joon
authors: YJ
authors: Ryu
authors: Hyang Sook
authors: HS
authors: Seol
authors: Tae Jun
authors: TJ
authors: Cho
authors: Tae Jung
authors: TJ
authors: Kwon
authors: Se Jin
authors: SJ
authors: Jang
authors: Jaejin
authors: J
authors: Cho
mesh_terms: D015415:Biomarkers / Q000032:analysis; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D016130:Immunophenotyping; D059630:Mesenchymal Stem Cells / Q000276:immunology* / Q000648:ultrastructure*; D046529:Microscopy, Electron, Transmission; D058628:Myofibroblasts / Q000276:immunology / Q000648:ultrastructure; D010641:Phenotype; D011247:Pregnancy; D014470:Umbilical Cord / Q000166:cytology / Q000276:immunology* / Q000648:ultrastructure*; D059631:Wharton Jelly / Q000166:cytology / Q000276:immunology* / Q000648:ultrastructure*
chemical_list: D015415:Biomarkers