title: Oral immunization with an attenuated Salmonella Gallinarum mutant as a fowl typhoid vaccine with a live adjuvant strain secreting the B subunit of Escherichia coli heat-labile enterotoxin.
abstract: BACKGROUND
  The Salmonella Gallinarum (SG) lon/cpxR deletion mutant JOL916 was developed as a live vaccine candidate for fowl typhoid (FT), and a SG mutant secreting an Escherichia coli heat-labile enterotoxin B subunit (LTB), designated JOL1229, was recently constructed as an adjuvant strain for oral vaccination against FT. In this study, we evaluated the immunogenicity and protective properties of the SG mutant JOL916 and the LTB adjuvant strain JOL1229 in order to establish a prime and boost immunization strategy for each strain. In addition, we compared the increase in body weight, the immunogenicity, the egg production rates, and the bacteriological egg contamination of these strains with those of SG 9R, a widely used commercial vaccine.
  
  
  RESULTS
  Plasma IgG, intestinal secretory IgA (sIgA), and cell-mediated responses were significantly induced after a boost inoculation with a mixture of JOL916 and JOL1229, and significant reductions in the mortality of chickens challenged with a wild-type SG strain were observed in the immunized groups. There were no significant differences in increases in body weight, cell-mediated immune responses, or systemic IgG responses between our vaccine mixture and the SG 9R vaccine groups. However, there was a significant elevation in intestinal sIgA in chickens immunized with our mixture at 3 weeks post-prime-immunization and at 3 weeks post-boost-immunization, while sIgA levels in SG 9R-immunized chickens were not significantly elevated compared to the control. In addition, the SG strain was not detected in the eggs of chickens immunized with our mixture.
  
  
  CONCLUSIONS
  Our results suggest that immunization with the LTB-adjuvant strain JOL1229 can significantly increase the immune response, and provide efficient protection against FT with no side effects on body weight, egg production, or egg contamination.
authors: College of Veterinary Medicine, Chonbuk National University, Jeonju 561-756, Republic of Korea.
authors: Byung Woo
authors: BW
authors: Jeon
authors: Rahul M
authors: RM
authors: Nandre
authors: John Hwa
authors: JH
authors: Lee
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000627:therapeutic use; D000284:Administration, Oral; D000818:Animals; D001427:Bacterial Toxins / Q000276:immunology* / Q000627:therapeutic use; D002645:Chickens / Q000276:immunology / Q000382:microbiology; D004768:Enterotoxins / Q000276:immunology* / Q000627:therapeutic use; D029968:Escherichia coli Proteins / Q000276:immunology* / Q000627:therapeutic use; D005260:Female; D007111:Immunity, Cellular / Q000276:immunology; D056724:Immunity, Humoral / Q000276:immunology; D010058:Oviposition / Q000276:immunology; D010063:Ovum / Q000382:microbiology; D011201:Poultry Diseases / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D012481:Salmonella Infections, Animal / Q000276:immunology / Q000517:prevention & control*; D019779:Salmonella enterica / Q000276:immunology*; D014436:Typhoid-Paratyphoid Vaccines / Q000008:administration & dosage / Q000627:therapeutic use*; D014613:Vaccines, Attenuated / Q000276:immunology / Q000627:therapeutic use
chemical_list: D000276:Adjuvants, Immunologic; D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; D014436:Typhoid-Paratyphoid Vaccines; D014613:Vaccines, Attenuated; C027538:heat-labile enterotoxin, E coli
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