title: Non-polyadenylated transcription in embryonic stem cells reveals novel non-coding RNA related to pluripotency and differentiation.
abstract: The transcriptional landscape in embryonic stem cells (ESCs) and during ESC differentiation has received considerable attention, albeit mostly confined to the polyadenylated fraction of RNA, whereas the non-polyadenylated (NPA) fraction remained largely unexplored. Notwithstanding, the NPA RNA super-family has every potential to participate in the regulation of pluripotency and stem cell fate. We conducted a comprehensive analysis of NPA RNA in ESCs using a combination of whole-genome tiling arrays and deep sequencing technologies. In addition to identifying previously characterized and new non-coding RNA members, we describe a group of novel conserved RNAs (snacRNAs: small NPA conserved), some of which are differentially expressed between ESC and neuronal progenitor cells, providing the first evidence of a novel group of potentially functional NPA RNA involved in the regulation of pluripotency and stem cell fate. We further show that minor spliceosomal small nuclear RNAs, which are NPA, are almost completely absent in ESCs and are upregulated in differentiation. Finally, we show differential processing of the minor intron of the polycomb group gene Eed. Our data suggest that NPA RNA, both known and novel, play important roles in ESCs.
authors: Hebrew University of Jerusalem, Jerusalem 91904, Israel.
authors: Ilana
authors: I
authors: Livyatan
authors: Arigela
authors: A
authors: Harikumar
authors: Malka
authors: M
authors: Nissim-Rafinia
authors: Radharani
authors: R
authors: Duttagupta
authors: Thomas R
authors: TR
authors: Gingeras
authors: Eran
authors: E
authors: Meshorer
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000378:metabolism*; D044127:Epigenesis, Genetic; D006657:Histones / Q000235:genetics; D008297:Male; D051379:Mice; D039904:Pluripotent Stem Cells / Q000378:metabolism*; D011506:Proteins / Q000235:genetics; D012319:RNA Polymerase II / Q000378:metabolism; D012326:RNA Splicing; D058727:RNA, Small Untranslated / Q000096:biosynthesis / Q000378:metabolism* / Q000502:physiology; D017381:Spliceosomes / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D006657:Histones; D011506:Proteins; D058727:RNA, Small Untranslated; D012319:RNA Polymerase II
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