title: Expression of non-replicating persistence associated genes of Mycobacterium bovis in lymph nodes from skin test-reactor cattle.
abstract: Upon oxygen shift-down, Mycobacterium tuberculosis complex bacteria can induce a genetic program characterized by halted duplication, which is called Non-replicating persistence (NRP). During this phase, at least 48 genes, collectively named Dormancy survival regulator (DosR) regulon, are important for the long-term survival of bacilli under a non-respiring state, a condition that bacilli encounter inside granulomatous lesions. It remains unclear whether expression of NRP genes occurs within the tissue of Mycobacterium bovis naturally infected cattle. In order to start dissecting this question, total RNA from bovine lymph node tissues of sacrificed tuberculin reacting animals was isolated and transcription of genes required for in vivo duplication (esxB and fbpB) and in vitro NRP (hspX, pfkB, and mb2660c) were analyzed by RT-PCR approaches. Detection of transcripts was positive in bovine tissue samples for genes hspX, pfkB, and mb2660c in 84, 32, and 21%, respectively. NRP genes were upregulated even in animals with a negative IFN-γ in vitro test, and the expression of NRP genes occurred more often than expression of the esxB gene.
authors: Centro de Investigación y Asistencia en Tecnología y diseño del Estado de Jalisco, A.C., Biotecnología Biomédica, Av. Normalistas 800, Col. Colinas de la Normal, C.P. 44270 Guadalajara, Jalisco, Mexico.
authors: B A
authors: BA
authors: Jiménez
authors: E
authors: E
authors: Hinojoza-Loza
authors: M A
authors: MA
authors: Flores-Valdez
authors: E
authors: E
authors: Prado-Montes de Oca
authors: K
authors: K
authors: Allen
authors: C
authors: C
authors: Estrada-Chávez
authors: S
authors: S
authors: Herrera-Rodríguez
authors: J M
authors: JM
authors: Flores-Fernández
authors: M
authors: M
authors: Martínez-Velázquez
authors: R
authors: R
authors: Hernández-Gutiérrez
authors: A H
authors: AH
authors: Alvarez
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002417:Cattle; D015964:Gene Expression Regulation, Bacterial; D007371:Interferon-gamma / Q000096:biosynthesis; D059425:Interferon-gamma Release Tests; D008198:Lymph Nodes / Q000382:microbiology*; D009163:Mycobacterium bovis / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism / Q000472:pathogenicity; D018085:Regulon; D012882:Skin Tests; D014374:Tuberculin Test; D014380:Tuberculosis, Bovine / Q000382:microbiology; D015854:Up-Regulation
chemical_list: D001426:Bacterial Proteins; D007371:Interferon-gamma
keywords: Cattle; DosR; Gene expression; Lymph nodes; Non-replicating persistence; TST reactors