title: A variant of blueberry necrotic ring blotch virus associated with red lesions in blueberry.
abstract: The complete genome of a variant of the multi-segmented (+) RNA virus blueberry necrotic ring blotch virus (BNRBV), which has not been assigned to a genus, was obtained from foliar red lesions on southern highbush blueberries grown in Alachua Co., Florida. The genome organization of this variant, BNRBV-RL, is the same as that of BNRBV: four genomic segments and seven ORFs (one ORF on each of RNA 1, RNA 2, and RNA 4 and as many as four ORFs on RNA 3). BLAST analysis revealed nucleic acid sequence identities of 89 %, 90 %, 90 % and 86 % to BNRBV RNA 1, RNA 2, RNA 3 and RNA 4, respectively. Phylogenetic analysis of the amino acid sequence of the putative RdRp domain indicated that BNRBV-RL was closely related to BNRBV and less related to citrus leprosis virus type C and three other mite-transmitted viruses. The nucleotide and amino acid sequence differences between BNRBV-RL and BNRBV combined with differences in symptom expression in blueberry would suggest that BNRBV-RL is a strain of BNRBV.
authors: Department of Plant Pathology, University of Florida, Gainesville, FL, USA.
authors: M
authors: M
authors: Cantu-Iris
authors: P F
authors: PF
authors: Harmon
authors: A
authors: A
authors: Londoño
authors: J E
authors: JE
authors: Polston
mesh_terms: D029798:Blueberry Plants / Q000821:virology*; D005638:Fruit / Q000821:virology*; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D014644:Genetic Variation; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D010942:Plant Viruses / Q000145:classification / Q000235:genetics*; D012367:RNA, Viral / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins