title: Determination by LC-MS of juvenile hormone titers in hemolymph of the silkworm, Bombyx mori.
abstract: Juvenile hormone (JH) I, II and III in the hemolymph of the silkworm, Bombyx mori were quantified by liquid chromatography-mass spectrometry (LC-MS). JHs were treated with methanol and trifluoroacetic acid to convert into JH methoxyhydrines (JH-MHs). The key to the analytical condition for JH-MHs was the addition of 5 µM sodium acetate to the eluting solution. Each JH-MH was observed as the sodium adduct ion with good sensitivity. This improved method enabled the titration of JH I, II and III in hemolymph of the silkworm to be monitored from the 3rd instar through to the early pupal stage. A peak of JH I was observed immediately after ecdysis in the 3rd and 4th instar stages. The JH I titer sharply decreased on day 1 and reached the lowest level before ecdysis, but there was no peak at the beginning of the 5th stadium, and no apparent increase was observed until pupation.
authors: Department of Life Science and Biotechnology, Faculty of Life and Environmental Science, Shimane University, Matsue, Shimane, Japan.
authors: Kenjiro
authors: K
authors: Furuta
authors: Akio
authors: A
authors: Ichikawa
authors: Mika
authors: M
authors: Murata
authors: Eiichi
authors: E
authors: Kuwano
authors: Tetsuro
authors: T
authors: Shinoda
authors: Takahiro
authors: T
authors: Shiotsuki
mesh_terms: D000818:Animals; D012831:Bombyx / Q000737:chemistry*; D002853:Chromatography, Liquid; D006458:Hemolymph / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007605:Juvenile Hormones / Q000032:analysis* / Q000737:chemistry; D013058:Mass Spectrometry; D000432:Methanol / Q000737:chemistry; D012492:Salts / Q000737:chemistry
chemical_list: D007605:Juvenile Hormones; D012492:Salts; D000432:Methanol