title: A dihydrochalcone and several homoisoflavonoids from Polygonatum odoratum are activators of adenosine monophosphate-activated protein kinase.
abstract: Adenosine monophosphate (AMP)-activated protein kinase (AMPK) is a major cellular energy sensor and master regulator of metabolic homeostasis; thus, AMPK plays a central role in studies on diabetes and related metabolic diseases. From the rhizomes of Polygonatum odoratum (Mill.) Druce, six homoisoflavonoids (1-6) and one dihydrochalcone (7) were isolated, and the structures of polygonatones A-D (4-7) were elucidated by various spectroscopic analyses. Compounds 1-7 were evaluated for their effect on AMPK activation. The amount of active phosphorylated AMPK and acetyl-CoA carboxylase in rat liver epithelial IAR-20 cells increased when the cells were incubated with the aforementioned compounds. Specifically, (3R)-5,7-dihydroxyl-6-methyl-8-methoxyl-3-(4'-hydroxylbenzyl)-chroman-4-one (1), (3R)-5,7-dihydroxyl-6,8-dimethyl-3-(4'-hydroxylbenzyl)-chroman-4-one (2), (3R)-5,7-dihydroxyl-6-methyl-3-(4'-hydroxylbenzyl)-chroman-4-one (3), and polygonatone D (7) exhibited significant activation effects.
authors: Institute of Materia Medica, Shandong Academy of Medical Sciences, Jinan, Shandong 250062, PR China.
authors: Huanjie
authors: H
authors: Guo
authors: Huanxin
authors: H
authors: Zhao
authors: Yuichiro
authors: Y
authors: Kanno
authors: Wei
authors: W
authors: Li
authors: Yanling
authors: Y
authors: Mu
authors: Xinzhu
authors: X
authors: Kuang
authors: Yoshio
authors: Y
authors: Inouye
authors: Kazuo
authors: K
authors: Koike
authors: Haipeng
authors: H
authors: Jiang
authors: Hong
authors: H
authors: Bai
mesh_terms: D041721:3T3-L1 Cells; D055372:AMP-Activated Protein Kinases / Q000737:chemistry / Q000378:metabolism*; D000103:Acetyl-CoA Carboxylase / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D047188:Chalcones / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D007529:Isoflavones / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D051379:Mice; D010766:Phosphorylation / Q000187:drug effects; D031384:Polygonatum / Q000737:chemistry*; D051381:Rats; D027343:Rhizome / Q000737:chemistry
chemical_list: D047188:Chalcones; D007529:Isoflavones; D055372:AMP-Activated Protein Kinases; D000103:Acetyl-CoA Carboxylase; C015812:dihydrochalcone