title: Lack of release of bound anthocyanins and phenolic acids from carrot plant cell walls and model composites during simulated gastric and small intestinal digestion.
abstract: Separately, polyphenols and plant cell walls (PCW) are important contributors to the health benefits associated with fruits and vegetables. However, interactions with PCW which occur either during food preparation or mastication may affect bioaccessibility and hence bioavailability of polyphenols. Binding interactions between anthocyanins, phenolic acids (PAs) and PCW components, were evaluated using both a bacterial cellulose-pectin model system and a black carrot puree system. The majority of available polyphenols bound to PCW material with 60-70% of available anthocyanins and PAs respectively binding to black carrot puree PCW matter. Once bound, release of polyphenols using acidified methanol is low with only ∼20% of total anthocyanins to ∼30% of PAs being released. Less than 2% of bound polyphenol was released after in vitro gastric and small intestinal (S.I.) digestion for both the model system and the black carrot puree PCW matter. Confocal laser scanning microscopy shows localised binding of anthocyanins to PCW. Very similar patterns of binding for anthocyanins and PAs suggest that PAs form complexes with anthocyanins and polysaccharides. Time dependent changes in extractability with acidified methanol but not the total bound fraction suggests that initial non-specific deposition on cellulose surfaces is followed by rearrangement of the bound molecules. Minimal release of anthocyanins and PAs after simulated gastric and S.I. digestion indicates that polyphenols in fruits and vegetables which bind to the PCW will be transported to the colon where they would be expected to be released by the action of cell wall degrading bacteria.
authors: ARC Centre of Excellence in Plant Cell Walls, Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD, Australia.
authors: Anneline
authors: A
authors: Padayachee
authors: Gabriele
authors: G
authors: Netzel
authors: Michael
authors: M
authors: Netzel
authors: Li
authors: L
authors: Day
authors: Deirdre
authors: D
authors: Mikkelsen
authors: Michael J
authors: MJ
authors: Gidley
mesh_terms: D016947:Acetobacteraceae / Q000737:chemistry / Q000378:metabolism; D000872:Anthocyanins / Q000737:chemistry / Q000378:metabolism*; D001682:Biological Availability; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism*; D002482:Cellulose / Q000737:chemistry / Q000378:metabolism; D018552:Daucus carota / Q000737:chemistry / Q000378:metabolism*; D004063:Digestion; D005638:Fruit / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism*; D006801:Humans; D062385:Hydroxybenzoates / Q000737:chemistry / Q000378:metabolism*; D007421:Intestine, Small / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D008954:Models, Biological; D013270:Stomach / Q000737:chemistry; D014675:Vegetables / Q000378:metabolism
chemical_list: D000872:Anthocyanins; D062385:Hydroxybenzoates; D002482:Cellulose; C017616:phenolic acid