title: Fermentation profiles of wheat dextrin, inulin and partially hydrolyzed guar gum using an in vitro digestion pretreatment and in vitro batch fermentation system model.
abstract: This study investigated the fermentation and microbiota profiles of three fibers, wheat dextrin (WD), partially hydrolyzed guar gum (PHGG), and inulin, since little is known about the effects of WD and PHGG on gut microbiota. A treatment of salivary amylase, pepsin, and pancreatin was used to better physiologic digestion. Fibers (0.5 g) were fermented in triplicate including a control group without fiber for 0, 4, 8, 12, and 24 h. Analysis of pH, gas volume, hydrogen and methane gases, and short chain fatty acid (SCFA) concentrations were completed at each time point. Quantitative polymerase chain reaction (qPCR) was used to measure Bifidobacteria and Lactobacillus CFUs at 24 h. WD produced the least gas during fermentation at 8, 12, and 24 h (P < 0.0001), while inulin produced the most by 8 h (P < 0.0001). Each fiber reached its lowest pH value at different time points with inulin at 8 h (mean ± SE) (5.94 ± 0.03), PHGG at 12 h (5.98 ± 0.01), and WD at 24 h (6.17 ± 0.03). All fibers had higher total SCFA concentrations compared to the negative control (P < 0.05) at 24 h. At 24 h, inulin produced significantly (P = 0.0016) more butyrate than WD with PHGG being similar to both. An exploratory microbial analysis (log(10) CFU/µL) showed WD had CFU for Bifidobacteria (6.12) and Lactobacillus (7.15) compared with the control (4.92 and 6.35, respectively). Rate of gas production is influenced by fiber source and may affect tolerance in vivo. Exploratory microbiota data hint at high levels of Bifidobacteria for WD, but require more robust investigation to corroborate these findings.
authors: Department of Food Science and Nutrition, University of Minnesota, 225 Food Science and Nutrition, 1334 Eckles Ave, St. Paul, MN 55108, USA. noack017@umn.edu
authors: Jackie
authors: J
authors: Noack
authors: Derek
authors: D
authors: Timm
authors: Ashok
authors: A
authors: Hospattankar
authors: Joanne
authors: J
authors: Slavin
mesh_terms: D000818:Animals; D001644:Bifidobacterium / Q000187:drug effects; D002087:Butyrates / Q000378:metabolism; D003912:Dextrins / Q000378:metabolism* / Q000494:pharmacology; D004032:Diet; D004043:Dietary Fiber / Q000378:metabolism* / Q000494:pharmacology; D004063:Digestion; D005232:Fatty Acids, Volatile / Q000378:metabolism*; D005285:Fermentation; D005685:Galactans / Q000378:metabolism* / Q000494:pharmacology; D005740:Gases; D041981:Gastrointestinal Tract / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006867:Hydrolases / Q000378:metabolism; D007444:Inulin / Q000378:metabolism* / Q000494:pharmacology; D007778:Lactobacillus / Q000187:drug effects; D008351:Mannans / Q000378:metabolism* / Q000494:pharmacology; D054892:Metagenome / Q000187:drug effects*; D008954:Models, Biological; D053149:Plant Gums / Q000378:metabolism* / Q000494:pharmacology; D016133:Polymerase Chain Reaction; D056692:Prebiotics; D013552:Swine; D014908:Triticum / Q000737:chemistry*
chemical_list: D002087:Butyrates; D003912:Dextrins; D004043:Dietary Fiber; D005232:Fatty Acids, Volatile; D005685:Galactans; D005740:Gases; D008351:Mannans; D053149:Plant Gums; D056692:Prebiotics; D007444:Inulin; C007894:guar gum; D006867:Hydrolases
references: Scand J Gastroenterol. 1989 Apr;24(3):359-67
references: 2544024
references: Mol Cancer Res. 2007 May;5(5):455-9
references: 17475672
references: Cancer Res. 1994 Jun 15;54(12):3288-93
references: 8205551
references: Br J Nutr. 2010 Aug;104 Suppl 2:S1-63
references: 20920376
references: PLoS One. 2011;6(7):e22448
references: 21829462
references: J Am Diet Assoc. 2010 Jun;110(6):865-8
references: 20497775
references: J Nutr Sci Vitaminol (Tokyo). 1994 Jun;40(3):251-9
references: 7965214
references: Clin Nutr. 2006 Feb;25(1):82-90
references: 16253403
references: J Am Diet Assoc. 1998 Aug;98(8):912-4
references: 9710664
references: J Agric Food Chem. 2009 Feb 25;57(4):1354-61
references: 19199596
references: J Med Food. 2010 Aug;13(4):961-6
references: 20482283
references: Am J Clin Nutr. 2001 Feb;73(2 Suppl):415S-420S
references: 11157351
references: Eur J Clin Nutr. 2004 Jul;58(7):1046-55
references: 15220947
references: J AOAC Int. 2012 Jan-Feb;95(1):50-60
references: 22468341
references: J Int Med Res. 2012;40(1):211-24
references: 22429361
references: J Food Sci. 2011 Jun-Jul;76(5):H137-42
references: 22417432
references: Gut. 2003 Dec;52(12):1708-13
references: 14633947
references: J Appl Bacteriol. 1993 Oct;75(4):373-80
references: 8226394
references: Gut. 1993 Apr;34(4):437-9
references: 8491386
references: Mol Nutr Food Res. 2006 Oct;50(10):971-6
references: 16967518