title: Assessment of key amino-acid residues of CD36 in specific binding interaction with an oxidized low-density lipoprotein.
abstract: CD36 binds oxidized low-density lipoprotein (oxLDL). A synthetic peptide comprising amino-acid residues 149-168 of mouse CD36 was recently found to bind fluorescence-labeled oxLDL particles. Based on our oxLDL-binding analysis of various synthetic CD36 peptides, we suggest that not only hydrophilic residues (e.g., Lys164 and Lys166) but also hydrophobic ones (e.g., Phe153, Leu158, and Leu161) are critical to binding.
authors: Laboratory of Nutrition Chemistry, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto University, Kyoto, Japan.
authors: Marie
authors: M
authors: Takai
authors: Satoshi
authors: S
authors: Tsuzuki
authors: Yukari
authors: Y
authors: Matsuno
authors: Yuki
authors: Y
authors: Kozai
authors: Ai
authors: A
authors: Eguchi
authors: Shigenobu
authors: S
authors: Matsumura
authors: Kazuo
authors: K
authors: Inoue
authors: Tohru
authors: T
authors: Fushiki
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001710:Biotin / Q000378:metabolism; D018955:CD36 Antigens / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008024:Ligands; D008077:Lipoproteins, LDL / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D013379:Substrate Specificity
chemical_list: D018955:CD36 Antigens; D008024:Ligands; D008077:Lipoproteins, LDL; D010446:Peptide Fragments; C099252:oxidized low density lipoprotein; D001710:Biotin