title: Establishment of odontoblastic cells, which indicate odontoblast features both in vivo and in vitro.
abstract: Tooth tissue engineering offers very attractive perspectives for elaboration of regenerative treatments, which enables to cure tooth loss and restore quality of life of the patients. To elaborate such treatment, isolation and culture of dental pulp cell must be achieved as a key element. In this article, we report the establishment of a stable cell line from GFP transgenic rat dental pulp, named TGC (Tooth Matrix-forming, GFP Rat-derived Cell). TGCs have exhibited odontoblastic feature both in vitro and in vivo. In vitro, TGC exposed to osteogenic medium demonstrated collagen fiber synthesis with matrix vesicle and mineralization and formed a sheet-like substrate on the cell culture dish. Increased ALP activity and elevated transcription level of various genes involved in calcification and dentin formation were also observed. In vivo, transplanted TGC in SCID mice with β-TCP particles formed dentin-like and pulp-like structure with lining odontoblast. Notably, even after up to 80 passages, TGCs retain their morphological features and differentiation ability. To our knowledge, this is the first report of a dental pulp-derived cell with such stable odontoblastic characteristics. TGC could be a very useful model for further study on dental pulp cell.
authors: Department of Oral Pathology and Medicine, Okayama University, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors: Hidetsugu
authors: H
authors: Tsujigiwa
authors: Naoki
authors: N
authors: Katase
authors: Mathieu
authors: M
authors: Lefeuvre
authors: Eiki
authors: E
authors: Yamachika
authors: Ryo
authors: R
authors: Tamamura
authors: Satoshi
authors: S
authors: Ito
authors: Yuichiro
authors: Y
authors: Takebe
authors: Hiroyuki
authors: H
authors: Matsuda
authors: Hitoshi
authors: H
authors: Nagatsuka
mesh_terms: D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002113:Calcification, Physiologic / Q000502:physiology; D002130:Calcium Phosphates / Q000737:chemistry; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D002478:Cells, Cultured; D003094:Collagen / Q000096:biosynthesis / Q000737:chemistry; D003470:Culture Media; D003782:Dental Pulp / Q000166:cytology* / Q000502:physiology; D003810:Dentinogenesis / Q000502:physiology; D004338:Drug Combinations; D016326:Extracellular Matrix Proteins / Q000032:analysis; D005260:Female; D005347:Fibroblasts / Q000502:physiology; D007797:Laminin / Q000737:chemistry; D008297:Male; D051379:Mice; D016513:Mice, SCID; D009804:Odontoblasts / Q000502:physiology* / Q000637:transplantation; D010012:Osteogenesis / Q000502:physiology; D010750:Phosphoproteins / Q000032:analysis; D011509:Proteoglycans / Q000737:chemistry; D051381:Rats; D055647:Rats, Transgenic; D012795:Sialoglycoproteins / Q000032:analysis; D040521:Subcutaneous Tissue / Q000601:surgery; D054457:Tissue Scaffolds / Q000737:chemistry; D000099311:Dentin Sialophosphoprotein
chemical_list: D000469:Alkaline Phosphatase; D001672:Biocompatible Materials; D002130:Calcium Phosphates; D003094:Collagen; D003470:Culture Media; D004338:Drug Combinations; D016326:Extracellular Matrix Proteins; D007797:Laminin; D010750:Phosphoproteins; D011509:Proteoglycans; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein; C513308:Dmp1 protein, mouse; C485817:beta-tricalcium phosphate; C059485:matrigel
keywords: dental pulp; matrix formation; osteoblast; stem cell-like cell