title: [TALE nuclease engineering and targeted genome modification].
abstract: Artificial designer nucleases targeting specific DNA sequences open up a new field for reverse genetics study. The rapid development of engineered endonucleases (EENs) enables targeted genome modification theoretically in any species. The construction of transcription activator-like effector nucleases (TALENs) is simpler with higher specificity and less toxicity than zinc-finger nucleases (ZFNs). Here, we summarized the recent progresses and prospects of TALEN technology, with an emphasis on its structure, function, and construction strategies, as well as a collection of species and genes that have been successfully modified by TALENs, especially the application in zebrafish.
authors: College of Life Sciences, Peking University, Beijing, China. yshen@pku.edu.cn
authors: Yan
authors: Y
authors: Shen
authors: An
authors: A
authors: Xiao
authors: Peng
authors: P
authors: Huang
authors: Wei-Ye
authors: WY
authors: Wang
authors: Zuo-Yan
authors: ZY
authors: Zhu
authors: Bo
authors: B
authors: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D004720:Endonucleases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D018390:Gene Targeting / Q000379:methods*; D016678:Genome / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D015202:Protein Engineering / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D011993:Recombinant Fusion Proteins; D004720:Endonucleases