title: Enhanced immune response and protective effects of nano-chitosan-based DNA vaccine encoding T cell epitopes of Esat-6 and FL against Mycobacterium tuberculosis infection.
abstract: Development of a novel and effective vaccine against Mycobacterium tuberculosis (M.tb) is a challenging for preventing TB infection. In this study, a novel nanoparticle-based recombinant DNA vaccine was developed, which contains Esat-6 three T cell epitopes (Esat-6/3e) and fms-like tyrosine kinase 3 ligand (FL) genes (termed Esat-6/3e-FL), and was enveloped with chitosan (CS) nanoparticles (nano-chitosan). The immunologic and protective efficacy of the nano-chitosan-based DNA vaccine (termed nano-Esat-6/3e-FL) was assessed in C57BL/6 mice after intramuscular prime vaccination with the plasmids DNA and nasal boost with the Esat-6/3e peptides. The results showed that the immunized mice remarkably elicited enhanced T cell responses and protection against M.tb H37Rv challenge. These findings indicate that the nano-chitosan can significantly elevate the immunologic and protective effects of the DNA vaccine, and the nano-Esat-6/3e-FL is a useful vaccine for preventing M.tb infection in mice.
authors: Department of Microbiology and Immunology, Nanjing Medical University, Nanjing, China.
authors: Ganzhu
authors: G
authors: Feng
authors: Qingtao
authors: Q
authors: Jiang
authors: Mei
authors: M
authors: Xia
authors: Yanlai
authors: Y
authors: Lu
authors: Wen
authors: W
authors: Qiu
authors: Dan
authors: D
authors: Zhao
authors: Liwei
authors: L
authors: Lu
authors: Guangyong
authors: G
authors: Peng
authors: Yingwei
authors: Y
authors: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000942:Antigens, Bacterial / Q000737:chemistry / Q000235:genetics*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D002452:Cell Count; D049109:Cell Proliferation; D048271:Chitosan / Q000737:chemistry*; D016207:Cytokines / Q000096:biosynthesis; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D005260:Female; D050990:GATA3 Transcription Factor / Q000378:metabolism; D005786:Gene Expression Regulation; D007114:Immunization; D007371:Interferon-gamma / Q000378:metabolism; D008024:Ligands; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009169:Mycobacterium tuberculosis / Q000276:immunology* / Q000502:physiology; D053758:Nanoparticles / Q000737:chemistry; D010316:Particle Size; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D013154:Spleen / Q000166:cytology; D020825:T-Box Domain Proteins / Q000378:metabolism; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D014162:Transfection; D014376:Tuberculosis / Q000517:prevention & control*; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology*; D051941:fms-Like Tyrosine Kinase 3 / Q000378:metabolism*; D000098815:T-bet Transcription Factor
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D016207:Cytokines; C093276:ESAT-6 protein, Mycobacterium tuberculosis; D018984:Epitopes, T-Lymphocyte; D050990:GATA3 Transcription Factor; D008024:Ligands; D011994:Recombinant Proteins; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D019444:Vaccines, DNA; D007371:Interferon-gamma; D048271:Chitosan; D051941:fms-Like Tyrosine Kinase 3
references: J Biomater Sci Polym Ed. 2006;17(12):1389-403
references: 17260510
references: Scand J Infect Dis. 2012 Mar;44(3):161-7
references: 21923628
references: Lancet. 2010 Jun 12;375(9731):2110-9
references: 20488515
references: Infect Immun. 2003 Jun;71(6):3058-67
references: 12761083
references: Scand J Immunol. 2010 Oct;72(4):332-8
references: 20883318
references: Vaccine. 2005 Mar 18;23(17-18):2121-31
references: 15755582
references: Immunol Cell Biol. 2008 Jan;86(1):98-106
references: 17909563
references: Biologicals. 2001 Sep-Dec;29(3-4):271-6
references: 11851327
references: Pharm Res. 2004 Feb;21(2):344-53
references: 15032318
references: Annu Rev Immunol. 2000;18:927-74
references: 10837079
references: Eur J Pharm Biopharm. 2004 Jan;57(1):123-31
references: 14729088
references: Eur J Immunol. 2006 Dec;36(12):3346-55
references: 17109467
references: Vaccine. 2008 Aug 18;26(35):4519-25
references: 18598729
references: J Immunol. 2008 Aug 15;181(4):2356-67
references: 18684925
references: Immunology. 2009 Dec;128(4):556-63
references: 19930045
references: J Immunol. 2006 Nov 1;177(9):6353-60
references: 17056566
references: J Immunol. 2009 Aug 15;183(4):2659-68
references: 19620314
references: J Control Release. 1998 Apr 30;53(1-3):183-93
references: 9741926
references: Vaccine. 2010 Apr 30;28(20):3571-81
references: 20226890
references: J Immunol. 2007 Sep 15;179(6):3973-81
references: 17785835
references: Infect Immun. 2010 Mar;78(3):1326-32
references: 20086089
references: Vaccine. 2011 Nov 8;29(48):8965-73
references: 21945255
references: Comp Immunol Microbiol Infect Dis. 2007 Jan;30(1):19-32
references: 17034859
references: Tuberculosis (Edinb). 2009 Nov;89(6):423-30
references: 19683472
references: Mol Pharm. 2012 Jan 1;9(1):81-90
references: 22149016
references: Pharm Res. 2002 Aug;19(8):1185-93
references: 12240945
references: J Immunol Methods. 1983 Dec 16;65(1-2):55-63
references: 6606682
references: Curr Opin Immunol. 2003 Aug;15(4):461-70
references: 12900280
references: Int J Pharm. 2008 Apr 16;354(1-2):235-41
references: 18182259
references: J Control Release. 2009 Sep 1;138(2):113-21
references: 19445980
references: Vaccine. 2010 Feb 3;28(5):1357-62
references: 19932674
references: Vaccine. 2007 May 10;25(19):3742-51
references: 17321015
references: J Immunol. 2001 May 1;166(9):5366-73
references: 11313372
references: J Control Release. 2001 Aug 10;75(3):409-19
references: 11489327
references: Adv Drug Deliv Rev. 2001 Sep 23;51(1-3):81-96
references: 11516781
references: Science. 2010 May 14;328(5980):856-61
references: 20466923
references: Vaccine. 2006 Mar 15;24(12):2017-26
references: 16414158
references: Microbiol Immunol. 2010 Aug;54(8):435-41
references: 20646207
references: J Immunol. 2005 Mar 15;174(6):3570-9
references: 15749894
references: Vaccine. 2011 Mar 3;29(11):2100-9
references: 21256189
references: Eur J Pharm Sci. 2012 Mar 12;45(4):475-81
references: 22009113
references: Infect Immun. 2000 Feb;68(2):791-5
references: 10639447
references: Nanomedicine. 2012 Nov;8(8):1337-44
references: 22406425
references: Microbiol Res. 2009;164(3):253-9
references: 17433643
references: Trends Microbiol. 2004 Nov;12(11):500-8
references: 15488391
references: Tuberculosis (Edinb). 2012 Jan;92(1):63-71
references: 22154006
references: Microbiol Immunol. 2009 Oct;53(10):541-9
references: 19780967
references: Trends Immunol. 2001 Jan;22(1):41-7
references: 11286691
references: PLoS Pathog. 2011 Nov;7(11):e1002378
references: 22102818
references: Paediatr Respir Rev. 2007 Jun;8(2):148-54
references: 17574159
references: Biomaterials. 2005 Oct;26(28):5727-36
references: 15878378