title: Effects of Copaifera duckei Dwyer oleoresin on the cell wall and cell division of Bacillus cereus.
abstract: The aim of this work was to evaluate the antibacterial activity of Copaifera duckei oleoresin and to determine its possible mechanism of action against bacteria of clinical and food interest. The antibacterial activity was determined by agar diffusion and dilution methods; the mechanism of action by transmission electron microscopy and by SDS-PAGE; the bioactive compounds by bioautography; and the chemical analysis by GC/MS. Oleoresin showed activity against nine of the 11 strains of bacteria tested. Bacillus cereus was the most sensitive, with a MIC corresponding to 0.03125 mg ml(-1) and with a bactericidal action. Oleoresin acted on the bacterial cell wall, removing proteins and the S-layer, and interfering with the cell-division process. This activity probably can be attributed to the action of terpenic compounds, among them the bisabolene compound. Gram-negative bacteria tested were not inhibited. C. duckei oleoresin is a potential antibacterial, suggesting that this oil could be used as a therapeutic alternative, mainly against B. cereus.
authors: Postgraduate program in Health Sciences, Federal University of Rio Grande do Norte, Natal-RN, Brazil.
authors: Elizabeth Cristina
authors: EC
authors: Gomes Dos Santos
authors: Department of Chemistry, Federal University of Minas Gerais, Belo Horizonte, Brazil.
authors: Claudio Luis
authors: CL
authors: Donnici
authors: Department of Morphology and Microscopy Center, Federal University of Minas Gerais, Belo Horizonte, Brazil.
authors: Elizabeth Ribeiro da Silva
authors: ERDS
authors: Camargos
authors: Department of Clinical and Toxicological Analysis, Federal University of Rio Grande do Norte, Natal-RN, Brazil.
authors: Adriana
authors: A
authors: Augusto de Rezende
authors: Department of Chemistry and Food, Federal University of Pará, Belém, Brazil.
authors: Eloisa Helena de Aguiar
authors: EHA
authors: Andrade
authors: Department of Pharmacy, Federal University of Pernambuco, Recife, Brazil.
authors: Luiz Alberto Lira
authors: LAL
authors: Soares
authors: Department of Microbiology Federal University of Minas Gerais, Belo Horizonte, Brazil.
authors: Luiz de Macêdo
authors: LM
authors: Farias
authors: Department of Microbiology Federal University of Minas Gerais, Belo Horizonte, Brazil.
authors: Maria Auxiliadora
authors: MA
authors: Roque de Carvalho
authors: Department of Clinical and Toxicological Analysis, Federal University of Rio Grande do Norte, Natal-RN, Brazil.
authors: Maria das Graças
authors: MDG
authors: Almeida
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D001409:Bacillus cereus / Q000166:cytology / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects*; D002473:Cell Wall / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D007887:Fabaceae / Q000737:chemistry* / Q000145:classification*; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology; D014640:Vancomycin / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D010938:Plant Oils; C018620:oleoresins; D014640:Vancomycin