title: Bioluminescent bioreporter for assessment of arsenic contamination in water samples of India.
abstract: In the present study the most efficient R-factor controlling the ars operon was selected after screening of 39 Escherichia coli isolates by minimum inhibitory concentration test (MIC) studies from water samples of different geographical locations of India. Among all, strain isolated from Hooghly River (West Bengal) was found to have maximum tolerance towards arsenic and was further used for the development of bioreporter bacteria. Cloning of the ars regulatory element along with operator-promotor and luxCDABE from Photobacteria into expression vector has been accomplished by following recombinant DNA protocols. The bioreporter sensor system developed in this study can measure the estimated range of 0.74-60 mu g of As/L and is both specific and selective for sensing bioavailable As. The constructed bacterial biosensor was further used for the determination of arsenic ion concentration in different environmental samples of India.
authors: Gene Expression Laboratory, Department of Biosciences, Jamia Millia Islamia University, New Delhi 110 025, India.
authors: Pratima
authors: P
authors: Sharma
authors: Shahzada
authors: S
authors: Asad
authors: Arif
authors: A
authors: Ali
mesh_terms: D001151:Arsenic / Q000032:analysis* / Q000494:pharmacology; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D002623:Chemistry Techniques, Analytical; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D017930:Genes, Reporter; D007194:India; D008163:Luminescent Measurements; D008826:Microbial Sensitivity Tests; D009876:Operon; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic; D014733:Vibrio / Q000235:genetics; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D014874:Water Pollutants, Chemical; D001151:Arsenic
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