title: F-box only protein 9 is required for adipocyte differentiation.
abstract: The objective of this study is to investigate whether F-box only protein 9 (FBXO9), an ubiquitination E3 ligase, has a functional role in adipocyte differentiation. Expression of FBXO9 was compared between obese mice and control lean mice using real-time PCR. Also, expression pattern of FBXO9 was monitored during 3T3-L1 adipocyte differentiation. FBXO9 was highly expressed in obese mice, and increased in the early stages of adipogenesis. To verify a functional role of FBXO9 in adipogenesis, FBXO9 was knocked down using transfection of siRNAs against FBXO9 into 3T3-L1 cells during the induction of adipogenesis. Knockdown of FBXO9 in early stage of adipogenesis almost completely inhibited adipogenesis, and CCAAT/enhancer binding protein β (C/EBPβ) levels were significantly reduced. However, the cells stably expressing C/EBPβ were fairly differentiated into adipocytes in the FBXO9 knockdown condition. These results suggest that FBXO9 is required for adipocyte differentiation, and C/EBPβ plays a role in the effect of FBXO9 on adipogenesis.
authors: Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Republic of Korea.
authors: Kyeong Won
authors: KW
authors: Lee
authors: Soo Heon
authors: SH
authors: Kwak
authors: Byung Yong
authors: BY
authors: Ahn
authors: Hak Mo
authors: HM
authors: Lee
authors: Hye Seung
authors: HS
authors: Jung
authors: Young Min
authors: YM
authors: Cho
authors: Young Joo
authors: YJ
authors: Park
authors: Sung Soo
authors: SS
authors: Chung
authors: Kyong Soo
authors: KS
authors: Park
mesh_terms: D017667:Adipocytes / Q000378:metabolism* / Q000473:pathology*; D050156:Adipogenesis; D000818:Animals; D002454:Cell Differentiation; D044783:F-Box Proteins / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000378:metabolism* / Q000473:pathology*
chemical_list: D044783:F-Box Proteins; C582681:FBXO9 protein, mouse