title: Assembly of MreB filaments on liposome membranes: a synthetic biology approach.
abstract: The physical interaction between the cytoskeleton and the cell membrane is essential in defining the morphology of living organisms. In this study, we use a synthetic approach to polymerize bacterial MreB filaments inside phospholipid vesicles. When the proteins MreB and MreC are expressed inside the liposomes, the MreB cytoskeleton structure develops at the inner membrane. Furthermore, when purified MreB is used inside the liposomes, MreB filaments form a 4-10 μm rigid bundle structure and deform the lipid vesicles in physical contact with the vesicle inner membrane. These results indicate that the fibrillation of MreB filaments can take place either in close proximity of deformable lipid membrane or in the presence of associated protein. Our finding might be relevant for the self-assembly of cytoskeleton filaments toward the construction of artificial cell systems.
authors: Yusuke T
authors: YT
authors: Maeda
authors: Tomoyoshi
authors: T
authors: Nakadai
authors: Jonghyeon
authors: J
authors: Shin
authors: Kunihiro
authors: K
authors: Uryu
authors: Vincent
authors: V
authors: Noireaux
authors: Albert
authors: A
authors: Libchaber
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008081:Liposomes / Q000378:metabolism; D008958:Models, Molecular; D055503:Protein Multimerization; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D058615:Synthetic Biology
chemical_list: D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D008081:Liposomes; C062061:MreC protein, Bacteria; D011993:Recombinant Fusion Proteins; C078473:MreB protein, E coli