title: Spectroscopic studies of the supramolecular interactions between uracil and 5-hydroxy-6-methyluracil with bovine serum albumin and its bilirubin complex.
abstract: Using fluorescence and absorption spectroscopy the interaction of bovine serum albumin and its bilirubin complex with uracil and 5-hydroxy-6-methyluracil in phosphate buffer at pH 7.4 was investigated. The parameters of forming intermolecular complexes (binding constants, quenching rate constants, the radius of the quenching sphere and etc.) were determined. The interaction between serum albumin and uracils is carried out by the static quenching of protein fluorescence and has predominantly hydrophobic character. Using synchronous fluorescence spectroscopy the influence of uracil and 5-hydroxy-6-methyluracil on the conformational changes of the protein molecule was studied. Uracils effectively binds to bilirubin-albumin complex compared to free protein, which is caused by the interaction with tetrapyrrolic pigment in macromolecular complex. Molecular docking calculations also being presented.
authors: Department of Inorganic Chemistry, Ivanovo State University of Chemistry and Technology (ISUCT), 7 Sheremetevsky prosp., 153000, Ivanovo, Russian Federation. Deus-Lex@yandex.ru
authors: Alexey V
authors: AV
authors: Solomonov
authors: Evgeniy V
authors: EV
authors: Rumyantsev
authors: Sergey P
authors: SP
authors: Ivanov
authors: Boris A
authors: BA
authors: Kochergin
authors: Elena V
authors: EV
authors: Antina
mesh_terms: D000818:Animals; D001663:Bilirubin / Q000737:chemistry*; D002417:Cattle; D057927:Hydrophobic and Hydrophilic Interactions; D062105:Molecular Docking Simulation; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D014498:Uracil / Q000031:analogs & derivatives* / Q000737:chemistry*; D056928:X-Ray Absorption Spectroscopy
chemical_list: D012710:Serum Albumin, Bovine; D014498:Uracil; C008378:6-methyluracil; D001663:Bilirubin
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