title: Transcriptome of the Lymantria dispar (gypsy moth) larval midgut in response to infection by Bacillus thuringiensis.
abstract: Transcriptomic profiles of the serious lepidopteran insect pest Lymantria dispar (gypsy moth) were characterized in the larval midgut in response to infection by Bacillus thuringiensis kurstaki, a biopesticide commonly used for its control. RNA-Seq approaches were used to define a set of 49,613 assembled transcript sequences, of which 838, 1,248 and 3,305 were respectively partitioned into high-, mid- and low-quality tiers on the basis of homology information. Digital gene expression profiles suggested genes differentially expressed at 24 hours post infection, and qRT-PCR analyses were performed for verification. The differentially expressed genes primarily associated with digestive function, including α-amylase, lipase and carboxypeptidase; immune response, including C-type lectin 4; developmental genes such as arylphorin; as well as a variety of binding proteins: cellular retinoic acid binding protein (lipid-binding), insulin-related peptide binding protein (protein-binding) and ovary C/EBPg transcription factor (nucleic acid-binding). This is the first study conducted to specifically investigate gypsy moth response to a bacterial infection challenge using large-scale sequencing technologies, and the results highlight important genes that could be involved in biopesticide resistance development or could serve as targets for biologically-based control mechanisms of this insect pest.
authors: United States Department of Agriculture -ARS Invasive Insect Biocontrol and Behavior Laboratory, Beltsville, Maryland, United States of America.
authors: Michael E
authors: ME
authors: Sparks
authors: Michael B
authors: MB
authors: Blackburn
authors: Daniel
authors: D
authors: Kuhar
authors: Dawn E
authors: DE
authors: Gundersen-Rindal
mesh_terms: D000818:Animals; D001413:Bacillus thuringiensis / Q000502:physiology*; D041981:Gastrointestinal Tract / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D005786:Gene Expression Regulation / Q000276:immunology; D063990:Gene Ontology; D054884:Host-Pathogen Interactions; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007814:Larva / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D009036:Moths / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D010572:Pest Control, Biological; D060888:Real-Time Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D059467:Transcriptome
chemical_list: D019476:Insect Proteins
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