title: Changes in leaf proteome profile of Arabidopsis thaliana in response to salicylic acid.
abstract: Salicylic acid (SA) has been implicated in determining the outcome of interactions between many plants and their pathogens. Global changes in response to this phytohormone have been observed at the transcript level, but little is known of how it induces changes in protein abundance. To this end we have investigated the effect of 1 mM SA on soluble proteins of Arabidopsis thaliana leaves by proteomic analysis. An initial study at transcript level has been performed on temporal landscape, which revealed that induction of most of the SA-responsive genes occurs within 3 to 6 h post treatment (HPT) and the expression peaked within 24 HPT. Two-dimensional gel electrophoresis (2-DE) coupled with MALDI-TOF MS/MS analysis has been used to identify differentially expressed proteins and 63 spots have been identified successfully. This comparative proteomic profiling of SA treated leaves versus control leaves demonstrated the changes of many defence related proteins like pathogenesis related protein 10a (PR10a), diseaseresistance- like protein, putative late blight-resistance protein, WRKY4, MYB4, etc. along with gross increase in the rate of energy production, while other general metabolism rate is slightly toned down, presumably signifying a transition from 'normal mode' to 'defence mode'.
authors: Plant Biology Laboratory, Drug Development/Diagnostics and Biotechnology Division, CSIRIndian Institute of Chemical Biology, Kolkata 700 032, India.
authors: Riddhi
authors: R
authors: Datta
authors: Ragini
authors: R
authors: Sinha
authors: Sharmila
authors: S
authors: Chattopadhyay
mesh_terms: D017360:Arabidopsis / Q000276:immunology / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D050260:Carbohydrate Metabolism; D060467:Disease Resistance; D004734:Energy Metabolism; D018506:Gene Expression Regulation, Plant; D058977:Molecular Sequence Annotation; D010937:Plant Growth Regulators / Q000494:pharmacology / Q000502:physiology*; D018515:Plant Leaves / Q000378:metabolism*; D014176:Protein Biosynthesis; D020543:Proteome / Q000378:metabolism*; D020156:Salicylic Acid / Q000378:metabolism* / Q000494:pharmacology; D013312:Stress, Physiological
chemical_list: D029681:Arabidopsis Proteins; D010937:Plant Growth Regulators; D020543:Proteome; D020156:Salicylic Acid
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