title: Development of Streptococcus gordonii-specific quantitative real-time polymerase chain reaction primers based on the nucleotide sequence of rpoB.
abstract: In this study, Streptococcus gordonii-specific quantitative real-time polymerase chain reaction (qPCR) primers, RTSgo-F2/RTSgo-R2, were developed based on the nucleotide sequences of RNA polymerase β-subunit gene (rpoB). The specificity of the RTSgo-F2/RTSgo-R2 primers was assessed by conventional PCR on 99 strains comprising 63 oral bacterial species, including the type strain and eight clinical isolates of S. gordonii. PCR products were amplified from the genomic DNAs of only S. gordonii strains. The qPCR primers were able to detect as little as 40 fg of S. gordonii genomic DNA at a cycle threshold value of 33. These findings suggest that these qPCR primers detect S. gordonii with high specificity and sensitivity.
authors: Korean Collection for Oral Microbiology, Department of Oral Biochemistry, School of Dentistry, Chosun University, 375 Seosuk-Dong, Dong-Gu, Gwangju, 501-759, Korea.
authors: Soon-Nang
authors: SN
authors: Park
authors: Joong-Ki
authors: JK
authors: Kook
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D015373:Bacterial Typing Techniques / Q000379:methods*; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity; D013290:Streptococcal Infections / Q000382:microbiology*; D054773:Streptococcus gordonii / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit
keywords: RNA polymerase β-subunit gene; Streptococcus gordonii; quantitative polymerase chain reaction primers; species-specific