title: [Effect of lumican gene over-expression on proliferation of lung adenocarcinoma cell A549 and its mechanism].
abstract: OBJECTIVE
  To investigate the effect of the lumican gene on the proliferation of lung adenocarcinoma cell A549 and the possible mechanism.
  
  
  METHODS
  The lung adenocarcinoma cell line A549 was infected with the recombinant lentivirus carrying the human lumican gene and a stable cell line was obtained via puromycin selection. The expression of lumican mRNA and protein was confirmed by fluorescence quantitative PCR(FQ-PCR) and Western blotting. We examined the cell growth curve, doubling time, proliferation index using MTT assay and flow cytometry and observed the status of the tumor in the experimental animals. Meanwhile, Western blotting was used to detect the expressions of RhoC and p-Akt proteins.
  
  
  RESULTS
  The A549 cell line with the lumican gene over-expression was successfully built. Compared with the control group and the empty vector group, the cells in the experimental group grew faster (P<0.05), the doubling time was shortened (P<0.05), the proliferation index went up (P<0.05), subcutaneous tumors in volume and mass increased and cells of the mitotic figures became more (P<0.05), and the expressions of RhoC and p-Akt proteins were raised (P<0.05). However, there was no obvious difference between the control group and the empty vecor group (P>0.05).
  
  
  CONCLUSIONS
  Lumican gene can promote the proliferation of lung adenocarcinoma cell A549, and its mechanism may be related to increased RhoC and p-Akt protein expressions.
authors: Department of Cardiothoracic Surgery, First Affiliated Hospital, Chongqing Medical University, China. zk05113073@163.com
authors: Kun
authors: K
authors: Zhou
authors: Mingjian
authors: M
authors: Ge
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D000818:Animals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D011508:Chondroitin Sulfate Proteoglycans / Q000235:genetics*; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D007632:Keratan Sulfate / Q000235:genetics*; D016086:Lentivirus / Q000235:genetics; D000071234:Lumican; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D014161:Transduction, Genetic; D014183:Transplantation, Heterologous; D047368:Tumor Burden / Q000235:genetics; D020741:rho GTP-Binding Proteins / Q000378:metabolism
chemical_list: D011508:Chondroitin Sulfate Proteoglycans; C000604306:LUM protein, human; C000604307:Lum protein, mouse; D000071234:Lumican; D007632:Keratan Sulfate; D051057:Proto-Oncogene Proteins c-akt; D020741:rho GTP-Binding Proteins