title: Ammonium and guanidinium dendron-carbon nanotubes by amidation and click chemistry and their use for siRNA delivery.
abstract: A series of multi-walled carbon nanotube (MWCNT) conjugates is described, functionalized with different dendrons bearing positive charges at their termini (i.e. ammonium or guanidinium groups). The dendrimeric units are anchored to the nanotube scaffolds using two orthogonal synthetic approaches, amidation and click reactions. The final nanohybrids are characterized by complementary analytical techniques, while their ability to interact with siRNA is investigated by means of agarose gel electrophoresis. The demonstration of the cell uptake capacity, the low cytotoxicity, and the ability of these cationic conjugates to silence cytotoxic genes suggests them to be promising carriers for genetic material.
authors: CNRS, Institut de Biologie Moléculaire et Cellulaire, Laboratoire d'Immunopathologie, et Chimie Thérapeutique, 67000 Strasbourg, France; Dipartimento di Scienze Chimiche e Farmaceutiche, Università di Trieste 34127, Trieste, Italy.
authors: Alessia
authors: A
authors: Battigelli
authors: Julie Tzu-Wen
authors: JT
authors: Wang
authors: Julie
authors: J
authors: Russier
authors: Tatiana
authors: T
authors: Da Ros
authors: Kostas
authors: K
authors: Kostarelos
authors: Khuloud T
authors: KT
authors: Al-Jamal
authors: Maurizio
authors: M
authors: Prato
authors: Alberto
authors: A
authors: Bianco
mesh_terms: D000577:Amides / Q000737:chemistry*; D064751:Ammonium Compounds / Q000737:chemistry*; D057930:Click Chemistry; D020868:Gene Silencing; D019791:Guanidine / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D037742:Nanotubes, Carbon / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000235:genetics
chemical_list: D000577:Amides; D064751:Ammonium Compounds; D037742:Nanotubes, Carbon; D034741:RNA, Small Interfering; D019791:Guanidine
keywords: RNA delivery; carbon nanotubes; cationic dendrons; gene therapy; siRNA