title: Tempe consumption modulates fecal secondary bile acids, mucins, immunoglobulin A, enzyme activities, and cecal microflora and organic acids in rats.
abstract: The present study investigated the effect of dietary tempe, a fermented soy product, on the colonic environment of rats fed high-fat (HF, 30 % fat; experiment 1) or low-fat (LF, 6 % fat; experiment 2) diets. Growing male rats were fed the experimental diets with or without 25 % tempe for 21 days. Tempe consumption slightly but significantly increased the growth of rats fed both the HF and LF diets (P < 0.05). With both the HF and LF diets, dietary tempe markedly reduced a harmful fecal secondary bile acid, lithocholic acid (a risk factor of colon cancer) (P < 0.05), and markedly elevated fecal mucins (indices of intestinal barrier function) and immunoglobulin A (IgA, an index of intestinal immune function) (P < 0.05). With the HF diet, dietary tempe increased cecal acetate, butyrate, propionate, and succinate concentrations (P < 0.05). Analysis of the profile of cecal microflora revealed lower Bacteroides and higher Clostridium cluster XIVa levels in the tempe group of rats fed the HF diet (P < 0.05). Compared with the control group, the fecal activity of β-glucosidase was markedly higher in the tempe group (P < 0.05), while that of urease was lower (P < 0.05) with both the HF and LF diets. The present results suggest that tempe consumption modulates the colonic environment in rats.
authors: Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Japan.
authors: Zaki
authors: Z
authors: Utama
authors: Yukako
authors: Y
authors: Okazaki
authors: Hiroyuki
authors: H
authors: Tomotake
authors: Norihisa
authors: N
authors: Kato
mesh_terms: D000085:Acetates / Q000378:metabolism; D000818:Animals; D001439:Bacteroides / Q000187:drug effects; D001647:Bile Acids and Salts / Q000378:metabolism*; D002087:Butyrates / Q000378:metabolism; D002432:Cecum / Q000187:drug effects / Q000382:microbiology*; D003013:Clostridium / Q000187:drug effects; D059305:Diet, High-Fat / Q000009:adverse effects; D004435:Eating / Q000187:drug effects; D004798:Enzymes / Q000378:metabolism*; D005243:Feces / Q000737:chemistry / Q000382:microbiology*; D007070:Immunoglobulin A / Q000378:metabolism; D008095:Lithocholic Acid / Q000378:metabolism; D008297:Male; D009077:Mucins / Q000378:metabolism*; D011422:Propionates / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D045730:Soy Foods; D013386:Succinates / Q000378:metabolism; D015430:Weight Gain / Q000187:drug effects
chemical_list: D000085:Acetates; D001647:Bile Acids and Salts; D002087:Butyrates; D004798:Enzymes; D007070:Immunoglobulin A; D009077:Mucins; D011422:Propionates; D013386:Succinates; D008095:Lithocholic Acid
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