title: Structural and functional characterization of the mumps virus phosphoprotein.
abstract: The phosphoprotein (P) is virally encoded by the Rhabdoviridae and Paramyxoviridae in the order Mononegavirales. P is a self-associated oligomer and forms complexes with the large viral polymerase protein (L), the nucleocapsid protein (N), and the assembled nucleocapsid. P from different viruses has shown structural diversities even though their essential functions are the same. We systematically mapped the domains in mumps virus (MuV) P and investigated their interactions with nucleocapsid-like particles (NLPs). Similar to other P proteins, MuV P contains N-terminal, central, and C-terminal domains with flexible linkers between neighboring domains. By pulldown assays, we discovered that in addition to the previously proposed nucleocapsid binding domain (residues 343 to 391), the N-terminal region of MuV P (residues 1 to 194) could also bind NLPs. Further analysis of binding kinetics was conducted using surface plasmon resonance. This is the first observation that both the N- and C-terminal regions of a negative-strand RNA virus P are involved in binding the nucleocapsid. In addition, we defined the oligomerization domain (POD) of MuV P as residues 213 to 277 and determined its crystal structure. The tetrameric MuV POD is formed by one pair of long parallel α-helices with another pair in opposite orientation. Unlike the parallel orientation of each α-helix in the tetramer of Sendai virus POD, this represents a novel orientation of a POD where both the N- and the C-terminal domains are at either end of the tetramer. This is consistent with the observation that both the N- and the C-terminal domains are involved in binding the nucleocapsid.
authors: Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama, USA.
authors: Robert
authors: R
authors: Cox
authors: Todd J
authors: TJ
authors: Green
authors: Sangeetha
authors: S
authors: Purushotham
authors: Champion
authors: C
authors: Deivanayagam
authors: Gregory J
authors: GJ
authors: Bedwell
authors: Peter E
authors: PE
authors: Prevelige
authors: Ming
authors: M
authors: Luo
mesh_terms: D019295:Computational Biology; D003460:Crystallization; D004926:Escherichia coli; D007700:Kinetics; D008958:Models, Molecular; D009109:Mumps virus / Q000235:genetics*; D019590:Nucleocapsid Proteins / Q000378:metabolism; D010750:Phosphoproteins / Q000737:chemistry* / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D020349:Surface Plasmon Resonance; D014961:X-Ray Diffraction
chemical_list: D019590:Nucleocapsid Proteins; D010750:Phosphoproteins
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