title: Cleavable trifunctional biotin reagents for protein labelling, capture and release.
abstract: Trifunctional biotin reagents incorporating cleavable linkers are evaluated for their usage in protein enrichment. A linker based on the Dde protecting group leads to efficient release of protein targets under mild conditions. It additionally contains a masked trypsin cleavage site, which eliminates the majority of the tag during tryptic digestion.
authors: Lehrstuhl für Chemie der Biopolymere, Technische Universität München, Weihenstephaner Berg 3, 85354 Freising, Germany.
authors: Yinliang
authors: Y
authors: Yang
authors: Steven H L
authors: SH
authors: Verhelst
mesh_terms: D001710:Biotin / Q000737:chemistry*; D007202:Indicators and Reagents / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D040901:Proteomics; D013194:Staining and Labeling
chemical_list: D007202:Indicators and Reagents; D011506:Proteins; D001710:Biotin