title: Osteoblastic cells culture on electrospun poly(ε-caprolacton) scaffolds incorporating amphiphilic PEG-POSS telechelic.
abstract: In this work, novel poly(ε-caprolactone) (PCL) fibrous membranes incorporating amphiphilic polyhedral oligosilsesquioxane (POSS) telechelic (PEG-POSS telechelic) were prepared via electrospinning. The unique microstructure, morphology, thermal stability of the resulting PCL/PEG-POSS telechelic electrospun nanowebs were investigated by X-ray diffraction, scanning electron microscopy, and thermogravimetric analysis, respectively. The addition of amphiphilic PEG-POSS telechelic strongly influenced the fiber diameters, microstructures of the resultant PCL/PEG-POSS telechelic nanofibers, compared to pure PCL nanofibers. The potential biomedical applications of such PEG-POSS telechelic nanowebs as a scaffolding material were also evaluated in vitro using mouse osteoblast-like MC3T3-E1 cells. The cell adhesion, spreading, and interaction behavior of pure PCL and PCL/PEG-POSS telechelic fibrous membranes were explored. It was found that electrospun PCL fibrous membranes incorporating amphiphilic PEG-POSS telechelic showed higher initial cell attachment than pure PCL due to the higher surface free energy of POSS siloxanes. Moreover, the obtained PCL/PEG-POSS telechelic fibrous scaffolds were found to be nontoxic and to maintain the good adhesion ratio between cells and surface (about ~93 %) after cell culturing for 24 h.
authors: Nano Fusion Technology Research Group, Interdisciplinary Graduate School of Science and Technology, Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano, 386-0015, Japan.
authors: Kyu-Oh
authors: KO
authors: Kim
authors: Byoung-Suhk
authors: BS
authors: Kim
authors: Ki-Hoon
authors: KH
authors: Lee
authors: Young-Hwan
authors: YH
authors: Park
authors: Ick-Soo
authors: IS
authors: Kim
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques / Q000295:instrumentation; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004595:Electroplating / Q000379:methods; D051379:Mice; D017646:Organosilicon Compounds / Q000737:chemistry* / Q000494:pharmacology; D010006:Osteoblasts / Q000166:cytology* / Q000187:drug effects / Q000502:physiology; D011091:Polyesters / Q000138:chemical synthesis / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000494:pharmacology; D013501:Surface-Active Agents / Q000737:chemistry / Q000494:pharmacology; D054457:Tissue Scaffolds / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D017646:Organosilicon Compounds; D011091:Polyesters; D013501:Surface-Active Agents; C521417:polyhedraloligosilsesquioxane; C016240:polycaprolactone; D011092:Polyethylene Glycols
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