title: Histone acetyltransferase 1 promotes homologous recombination in DNA repair by facilitating histone turnover.
abstract: Faithful repair of DNA double-strand breaks is vital to the maintenance of genome integrity and proper cell functions. Histone modifications, such as reversible acetylation, phosphorylation, methylation, and ubiquitination, which collectively contribute to the establishment of distinct chromatin states, play important roles in the recruitment of repair factors to the sites of double-strand breaks. Here we report that histone acetyltransferase 1 (HAT1), a classical B type histone acetyltransferase responsible for acetylating the N-terminal tail of newly synthesized histone H4 in the cytoplasm, is a key regulator of DNA repair by homologous recombination in the nucleus. We found that HAT1 is required for the incorporation of H4K5/K12-acetylated H3.3 at sites of double-strand breaks through its HIRA-dependent histone turnover activity. Incorporated histones with specific chemical modifications facilitate subsequent recruitment of RAD51, a key repair factor in mammalian cells, to promote efficient homologous recombination. Significantly, depletion of HAT1 sensitized cells to DNA damage compromised the global chromatin structure, inhibited cell proliferation, and induced cell apoptosis. Our experiments uncovered a role for HAT1 in DNA repair in higher eukaryotic organisms and provide a mechanistic insight into the regulation of histone dynamics by HAT1.
authors: Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Biochemistry and Molecular Biology, Peking University Health Science Center, Beijing 100191, China.
authors: Xiaohan
authors: X
authors: Yang
authors: Lei
authors: L
authors: Li
authors: Jing
authors: J
authors: Liang
authors: Lei
authors: L
authors: Shi
authors: Jianguo
authors: J
authors: Yang
authors: Xia
authors: X
authors: Yi
authors: Di
authors: D
authors: Zhang
authors: Xiao
authors: X
authors: Han
authors: Na
authors: N
authors: Yu
authors: Yongfeng
authors: Y
authors: Shang
mesh_terms: D017766:Acid Anhydride Hydrolases; D000595:Amino Acid Sequence; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002843:Chromatin / Q000235:genetics / Q000378:metabolism; D004260:DNA Repair; D045643:DNA Repair Enzymes / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D045325:HCT116 Cells; D057809:HEK293 Cells; D006367:HeLa Cells; D051548:Histone Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D059765:Homologous Recombination; D006801:Humans; D061986:MCF-7 Cells; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D011485:Protein Binding; D034622:RNA Interference; D051135:Rad51 Recombinase / Q000378:metabolism
chemical_list: D002843:Chromatin; D004268:DNA-Binding Proteins; C468783:H2AX protein, human; D006657:Histones; D051548:Histone Acetyltransferases; C110880:histone acetyltransferase type B complex; D051135:Rad51 Recombinase; D017766:Acid Anhydride Hydrolases; C112158:RAD50 protein, human; D045643:DNA Repair Enzymes
keywords: DNA Damage Response; DNA Repair; Histone Acetylase; Histone Chaperone; Histone Modification
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