title: Glucosinolate degradation products, isothiocyanates, nitriles, and thiocyanates, induce stomatal closure accompanied by peroxidase-mediated reactive oxygen species production in Arabidopsis thaliana.
abstract: Isothiocyanates, nitriles, and thiocyanates are degradation products of glucosinolates in crucifer plants. In this study, we investigated the stomatal response to allyl isothiocyanate (AITC), 3-butenenitrile (3BN), and ethyl thiocyanate (ESCN) in Arabidopsis. AITC, 3BN, and ESCN induced stomatal closure in the wild type and the atrbohD atrbohF mutant. Stomatal closure was inhibited by catalase and salicylhydroxamic acid (SHAM). The degradation products induced extracellular reactive oxygen species (ROS) production in the rosette leaves, and intracellular ROS accumulation, NO production, and cytosolic free calcium concentration ([Ca(2+)]cyt) oscillations in guard cells, which were inhibited by SHAM. These results suggest that glucosinolate degradation products induce stomatal closure accompanied by extracellular ROS production mediated by SHAM-sensitive peroxidases, intracellular ROS accumulation, and [Ca(2+)]cyt oscillation in Arabidopsis.
authors: Graduate School of Natural Science and Technology, Division of Bioscience, Okayama University, Okayama, Japan.
authors: Mohammad Shakhawat
authors: MS
authors: Hossain
authors: Wenxiu
authors: W
authors: Ye
authors: Mohammad Anowar
authors: MA
authors: Hossain
authors: Eiji
authors: E
authors: Okuma
authors: Misugi
authors: M
authors: Uraji
authors: Yoshimasa
authors: Y
authors: Nakamura
authors: Izumi C
authors: IC
authors: Mori
authors: Yoshiyuki
authors: Y
authors: Murata
mesh_terms: D017360:Arabidopsis / Q000033:anatomy & histology / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D005961:Glucosinolates / Q000378:metabolism*; D017879:Isothiocyanates / Q000378:metabolism / Q000494:pharmacology*; D009569:Nitric Oxide / Q000096:biosynthesis; D009570:Nitriles / Q000378:metabolism / Q000494:pharmacology*; D009195:Peroxidase / Q000378:metabolism; D018515:Plant Leaves / Q000166:cytology / Q000187:drug effects; D054046:Plant Stomata / Q000033:anatomy & histology / Q000187:drug effects*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013861:Thiocyanates / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D005961:Glucosinolates; D017879:Isothiocyanates; D009570:Nitriles; D017382:Reactive Oxygen Species; D013861:Thiocyanates; C037152:isothiocyanic acid; D009569:Nitric Oxide; C015660:allyl cyanide; D009195:Peroxidase; C031760:thiocyanate; D002118:Calcium