title: Inactivation of Tor proteins affects the dynamics of endocytic proteins in early stage of endocytosis.
abstract: Tor2 is an activator of the Rom2/Rho1 pathway that regulates alpha-factor internalization. Since the recruitment of endocytic proteins such as actin-binding proteins and the amphiphysins precedes the internalization of alpha-factor, we hypothesized that loss of Tor function leads to an alteration in the dynamics of the endocytic proteins. We report here that endocytic proteins, Abp1 and Rvs167, are less recruited to endocytic sites not only in tor2 but also tor1 mutants. Furthermore, we found that the endocytic proteins Rvs167 and Sjl2 are completely mistargeted to the cytoplasm in tor1 delta tor2ts double mutant cells. We also demonstrate here that the efficiency of endocytic internalization or scission in all tor mutants was drastically decreased. In agreement with the Sjl2 mislocalization, we found that in tor1 delta tor2ts double mutant cells, as well as other tor mutant cells, the overall PIP2 level was dramatically increased. Finally, the cell wall chitin content in tor2ts and tor1 delta tor2ts mutant cells was also significantly increased. Taken together, both functional Tor proteins, Tor1 and Tor2, are essentially required for proper endocytic protein dynamics at the early stage of endocytosis.
authors: Department of Biology, Missouri State University, 901 South National Ave, Springfield, MO 65897, USA.
authors: Brandon
authors: B
authors: Tenay
authors: Evin
authors: E
authors: Kimberlin
authors: Michelle
authors: M
authors: Williams
authors: Juliette
authors: J
authors: Denise
authors: Joshua
authors: J
authors: Fakilahyel
authors: Kyoungtae
authors: K
authors: Kim
mesh_terms: D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002686:Chitin / Q000378:metabolism; D004705:Endocytosis; D055786:Gene Knockout Techniques; D008840:Microfilament Proteins / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics / Q000378:metabolism*; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000378:metabolism; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism; D021381:Protein Transport; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D018797:Cell Cycle Proteins; D002686:Chitin; D008840:Microfilament Proteins; D019869:Phosphatidylinositol 3-Kinases; D019269:Phosphatidylinositol 4,5-Diphosphate; D010744:Phosphoric Monoester Hydrolases; D029701:Saccharomyces cerevisiae Proteins; C062612:ABP1 protein, S cerevisiae; C082282:RVS167 protein, S cerevisiae; C083324:TOR1 protein, S cerevisiae; C081135:TOR2 protein, S cerevisiae; C110552:INP52 protein, S cerevisiae
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