title: [In vitro effect of the S3Pvac vaccine against cysticercosis in human mononucleate cells].
abstract: BACKGROUND
  Neurocysticercosis (NCC) is a parasitic infection caused by the establishment of Taenia solium cysticerci in the central nervous system. The larval stage of the parasite also affects the pig, which is the essential intermediate host for transmission. For this reason, many researchers have focused on identifying protective antigens to prevent swine cysticercosis and interrupt the transmission. These include S3Pvac vaccine antigens. Vaccine is constituted by three protective synthetic peptides: KETc1, KETc12 and GK1. AIM. To evaluate the effect of the vaccine peptides KETc1, KETc12 and GK1 in mononuclear cells of patients with neuro-cysticercosis and healthy individuals.
  
  
  METHODS
  Comparative, prospective, transverse study. We studied the proliferation and cytokine profile induced by the three peptides in mononuclear cells from three patients with active NCC, 16 patients by calcified NCC and 16 healthy subjects.
  
  
  RESULTS
  KETc1 induces low levels of proliferation in cells from patients with active and controlled NCC, both in lymphocytes and in monocytes. KETc12 and GK-1 induce positive proliferation levels of monocytes in healthy subjects.
  
  
  CONCLUSIONS
  KETc1 peptide could be used as an adjuvant in the treatment of patients with active NCC, as induced a Th2 response also GK1 peptide as stimulator of monocyte/macrophage in immunizations with other proteins.
authors: Benemerita Universidad Autonoma de Puebla, Puebla, Mexico. diazorea@yahoo.com.mx
authors: María Alicia
authors: MA
authors: Díaz-Orea
authors: José Miguel
authors: JM
authors: Mijares
authors: Raúl
authors: R
authors: Arcega
authors: Eduardo
authors: E
authors: Gómez-Conde
authors: Víctor Omar
authors: VO
authors: Castellanos-Sánchez
authors: Rosendo
authors: R
authors: Briones-Rojas
authors: Juan Carlos
authors: JC
authors: Flores-Alonso
authors: Miguel Ángel
authors: MÁ
authors: Marín-Briones
authors: Gerardo
authors: G
authors: Santos-López
mesh_terms: D000276:Adjuvants, Immunologic; D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis / Q000276:immunology*; D002114:Calcinosis / Q000097:blood / Q000276:immunology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003430:Cross-Sectional Studies; D003552:Cysticercus / Q000276:immunology* / Q000648:ultrastructure; D004353:Drug Evaluation, Preclinical; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000378:metabolism; D007378:Interleukins / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D020019:Neurocysticercosis / Q000097:blood / Q000276:immunology*; D010446:Peptide Fragments / Q000138:chemical synthesis / Q000276:immunology; D011446:Prospective Studies; D022223:Vaccines, Subunit / Q000276:immunology / Q000494:pharmacology*; D055815:Young Adult
chemical_list: D000276:Adjuvants, Immunologic; D000947:Antigens, Helminth; D007378:Interleukins; D010446:Peptide Fragments; D022223:Vaccines, Subunit; D007371:Interferon-gamma