title: Correlation of total antioxidant capacity with reactive oxygen species (ROS) consumption measured by oxidative conversion.
abstract: Although both antioxidant capacity and oxidative conversion (hazard) are important in food and bioanalytical chemistry, there is considerable confusion in the literature between the results of these two types of assays. After the generation of ROS in the medium via Fe(III)-H₂O₂ reaction, attenuation of total oxidative conversion (TOC; as measured by thiobarbituric acid-reactive substances (TBARS) and N,N-dimethyl-p-phenylenediamine (DMPD) assays) was tested for possible correlation with the total antioxidant capacity (TAC; as measured by cupric reducing antioxidant capacity (CUPRAC) and trolox equivalent antioxidant capacity (ABTS/TEAC) assays) of the introduced antioxidant sample. The inverse relationship between oxidative conversion and antioxidant capacity was processed to establish a curvilinear relationship between the absolute values of TAC increments and TOC decrements as a function of added antioxidant concentration. This simple relationship may form a bridge between the two diverse disciplines of medical biochemistry and food analytical chemistry mainly using TOC and TAC results, respectively.
authors: Department of Chemistry, Faculty of Engineering, Istanbul University , 34320 Istanbul, Turkey.
authors: Sema Demirci
authors: SD
authors: Çekiç
authors: Aydan
authors: A
authors: Çetinkaya
authors: Aslı Neslihan
authors: AN
authors: Avan
authors: Reşat
authors: R
authors: Apak
mesh_terms: D000975:Antioxidants / Q000032:analysis* / Q000737:chemistry; D002614:Chelating Agents / Q000737:chemistry; D002839:Chromans / Q000737:chemistry; D005504:Food Analysis; D016166:Free Radical Scavengers / Q000032:analysis* / Q000737:chemistry; D007202:Indicators and Reagents / Q000737:chemistry; D007700:Kinetics; D008956:Models, Chemical; D010084:Oxidation-Reduction; D010618:Phenanthrolines / Q000737:chemistry; D010655:Phenylenediamines / Q000032:analysis / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors* / Q000737:chemistry; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis
chemical_list: D000975:Antioxidants; D002614:Chelating Agents; D002839:Chromans; D016166:Free Radical Scavengers; D007202:Indicators and Reagents; D010618:Phenanthrolines; D010655:Phenylenediamines; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; C004781:dimethyl-4-phenylenediamine; C002701:neocuproine; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid