title: Existence of lysogenic bacteriophages in Bacillus thuringiensis type strains.
abstract: We screened the existence of bacteriophages in 67 Bacillus thuringiensis type strains by phage DNA extraction and PCR using phage terminase small subunit (TerS)-specific primers to the supernatants and the precipitated pellets of Bt cultures, and by transmission electron microscopy. The various bacteriophages were observed from the supernatants of 22 type strains. Ten type strains showed the extracted phage DNAs and the amplified fragment by TerS PCR but 12 type strains showed only the phage DNAs. Their morphological characteristic suggests that they belong to Family Siphoviridae which had a long tail and symmetrical head.
authors: Department of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University, Seoul 151-742, Republic of Korea. rohling@korea.kr
authors: Jong Yul
authors: JY
authors: Roh
authors: Jong Bin
authors: JB
authors: Park
authors: Qin
authors: Q
authors: Liu
authors: Song Eun
authors: SE
authors: Kim
authors: Xueying
authors: X
authors: Tao
authors: Tae Woong
authors: TW
authors: Choi
authors: Jae Young
authors: JY
authors: Choi
authors: Woo Jin
authors: WJ
authors: Kim
authors: Byung Rae
authors: BR
authors: Jin
authors: Yeon Ho
authors: YH
authors: Je
mesh_terms: D017103:Bacillus Phages / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure; D001413:Bacillus thuringiensis / Q000235:genetics / Q000821:virology*; D004279:DNA, Viral / Q000737:chemistry; D046529:Microscopy, Electron, Transmission; D016133:Polymerase Chain Reaction; D017906:Siphoviridae / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure
chemical_list: D004279:DNA, Viral