title: Global spread and genetic variants of the two CYP9M10 haplotype forms associated with insecticide resistance in Culex quinquefasciatus Say.
abstract: Insecticide resistance develops as a genetic factor (allele) conferring lower susceptibility to insecticides proliferates within a target insect population under strong positive selection. Intriguingly, a resistance allele pre-existing in a population often bears a series of further adaptive allelic variants through new mutations. This phenomenon occasionally results in replacement of the predominating resistance allele by fitter new derivatives, and consequently, development of greater resistance at the population level. The overexpression of the cytochrome P450 gene CYP9M10 is associated with pyrethroid resistance in the southern house mosquito Culex quinquefasciatus. Previously, we have found two genealogically related overexpressing CYP9M10 haplotypes, which differ in gene copy number (duplicated and non-duplicated). The duplicated haplotype was derived from the non-duplicated overproducer probably recently. In the present study, we investigated allelic series of CYP9M10 involved in three C. quinquefasciatus laboratory colonies recently collected from three different localities. Duplicated and non-duplicated overproducing haplotypes coexisted in African and Asian colonies indicating a global distribution of both haplotype lineages. The duplicated haplotypes both in the Asian and African colonies were associated with higher expression levels and stronger resistance than non-duplicated overproducing haplotypes. There were slight variation in expression level among the non-duplicated overproducing haplotypes. The nucleotide sequences in coding and upstream regions among members of this group also showed a little diversity. Non-duplicated overproducing haplotypes with relatively higher expression were genealogically closer to the duplicated haplotypes than the other non-duplicated overproducing haplotypes, suggesting multiple cis-acting mutations before duplication.
authors: Department of Medical Entomology, National Institute of Infectious Diseases, Tokyo, Japan.
authors: K
authors: K
authors: Itokawa
authors: O
authors: O
authors: Komagata
authors: S
authors: S
authors: Kasai
authors: H
authors: H
authors: Kawada
authors: C
authors: C
authors: Mwatele
authors: G O
authors: GO
authors: Dida
authors: S M
authors: SM
authors: Njenga
authors: C
authors: C
authors: Mwandawiro
authors: T
authors: T
authors: Tomita
mesh_terms: D000483:Alleles; D000818:Animals; D003465:Culex / Q000145:classification / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism; D014644:Genetic Variation; D006239:Haplotypes; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007305:Insecticide Resistance; D007306:Insecticides / Q000494:pharmacology*; D010802:Phylogeny
chemical_list: D019476:Insect Proteins; D007306:Insecticides; D003577:Cytochrome P-450 Enzyme System
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