title: Cytotoxic and clastogenic activity of CdCl2 in human lymphocytes from different donors.
abstract: The sensitivity of human lymphocytes from different donors to CdCl2 using a complex of methods for determination of cytotoxicity and genotoxicity was studied. As endpoints for cytotoxicity the mitotic index (MI) and apoptosis were evaluated. To indicate genotoxicity chromosome aberrations test (CA) was used. The results indicate an individual sensitivity of lymphocytes to CdCl2-induced damage, which is directly depending on the concentration (10(-6), 10(-5), 5×10(-5), 10(-4)mol/l) applied. The assessment of the toxic and genotoxic effect using various endpoints allows more complete risk estimation for organisms exposed to heavy metals. The results have direct practical significance for threat evaluation in humans.
authors: Department of Environmental Mutagenesis and Genetic Risk Assessment, Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences, 2 Gagarin Street, Sofia 1113, Bulgaria. spetkova2002@yahoo.co.uk
authors: Svetla
authors: S
authors: Gateva
authors: Gabriele
authors: G
authors: Jovtchev
authors: Mila
authors: M
authors: Stergios
mesh_terms: D000328:Adult; D019256:Cadmium Chloride / Q000633:toxicity*; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000139:chemically induced*; D003603:Cytotoxins / Q000633:toxicity*; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D008940:Mitotic Index; D009153:Mutagens / Q000633:toxicity*
chemical_list: D003603:Cytotoxins; D009153:Mutagens; D019256:Cadmium Chloride