title: The effects of pore size in bilayered poly(lactide-co-glycolide) scaffolds on restoring osteochondral defects in rabbits.
abstract: Bilayered porous scaffolds have recently attracted interest because of their considerable promise for repairing osteochondral defects. However, determination of optimal pore size in bilayered porous scaffolds remains an important issue. This study investigated the in vivo effects of pore size in bilayered scaffolds using a rabbit model of osteochondral defects. We fabricated five types of integrated bilayered poly(lactide-co-glycolide) (PLGA) scaffolds with different pore sizes in the chondral and osseous layers (50-100 µm, 100-200 µm, 200-300 µm, and 300-450 µm). A subset of bilayered scaffolds seeded with or without allogenic bone marrow mesenchymal stem cells (BMSCs) was implanted in rabbit osteochondral defects. All of the cell/scaffold composite constructs supported the simultaneous regeneration of articular cartilage and subchondral bone, but the best results were observed in cell-seeded PLGA scaffolds with 100-200 µm pores in the chondral layer and 300-450 µm pores in the osseous layer. Our study supports the concept that the effects of pore size on osteochondral repair should be taken into consideration during scaffold design for tissue engineering.
authors: Department of Orthopaedic Surgery, Zhongshan Hospital, State Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai 200032, China.
authors: Pingguo
authors: P
authors: Duan
authors: Zhen
authors: Z
authors: Pan
authors: Lu
authors: L
authors: Cao
authors: Yao
authors: Y
authors: He
authors: Huiren
authors: H
authors: Wang
authors: Zehua
authors: Z
authors: Qu
authors: Jian
authors: J
authors: Dong
authors: Jiandong
authors: J
authors: Ding
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D001842:Bone and Bones / Q000293:injuries* / Q000378:metabolism / Q000473:pathology; D002358:Cartilage, Articular / Q000293:injuries* / Q000378:metabolism / Q000473:pathology; D011098:Polyglactin 910 / Q000737:chemistry / Q000494:pharmacology; D016062:Porosity; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D011098:Polyglactin 910
keywords: bilayered scaffold; osteochondral defect; poly(lactide‐co‐glycolide); stem cell; tissue engineering