title: SERS-based DNA detection in aqueous solutions using oligonucleotide-modified Ag nanoprisms and gold nanoparticles.
abstract: Oligonucleotide-modified nanoparticle conjugates show highly promising potential for SERS-based DNA detection. However, it remains challenging to carry out the SERS-based DNA detection in aqueous solutions directly using oligonucleotide-modified nanoparticles, because the Raman reporters would exhibit lower signals when they are dispersed in aqueous solutions than laid on "dry" metal nanoparticles. Here, we synthesized stable oligonucleotide-modified Ag nanoprism conjugates, and performed SERS-based DNA detection in aqueous solution directly by using such conjugates in combination with Raman reporter-labeled, oligonucleotide-modified gold nanoparticles. The experimental results indicate that this SERS-based DNA detection approach exhibited a good linear correlation between SERS signal intensity and the logarithm of target DNA concentration ranging from 10(-11)∼10(-8) M. This sensitivity is comparable to those SERS-based DNA detection approaches with the "dry" process. Additionally, a similar correlation could also be observed in duplex target DNA detection by SERS hybrid probes. Our results suggest that the oligonucleotide-modified Ag nanoprisms may be developed as a powerful SERS-based DNA detection tool.
authors: Advanced Photonics Center, Southeast University, 2# Sipai Lou, Nanjing, 210096, Jiangsu, China.
authors: Min
authors: M
authors: Liu
authors: Zhuyuan
authors: Z
authors: Wang
authors: Shenfei
authors: S
authors: Zong
authors: Ruohu
authors: R
authors: Zhang
authors: Dan
authors: D
authors: Zhu
authors: Shuhong
authors: S
authors: Xu
authors: Chunlei
authors: C
authors: Wang
authors: Yiping
authors: Y
authors: Cui
mesh_terms: D004247:DNA / Q000032:analysis*; D015342:DNA Probes; D006046:Gold; D053768:Metal Nanoparticles; D053761:Nanocomposites / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012834:Silver; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*
chemical_list: D015342:DNA Probes; D009841:Oligonucleotides; D012834:Silver; D006046:Gold; D004247:DNA