title: Ubiquitin-specific proteases UBP12 and UBP13 act in circadian clock and photoperiodic flowering regulation in Arabidopsis.
abstract: Protein ubiquitination is involved in most cellular processes. In Arabidopsis (Arabidopsis thaliana), ubiquitin-mediated protein degradation regulates the stability of key components of the circadian clock feedback loops and the photoperiodic flowering pathway. Here, we identified two ubiquitin-specific proteases, UBP12 and UBP13, involved in circadian clock and photoperiodic flowering regulation. Double mutants of ubp12 and ubp13 display pleiotropic phenotypes, including early flowering and short periodicity of circadian rhythms. In ubp12 ubp13 double mutants, CONSTANS (CO) transcript rises earlier than that of wild-type plants during the day, which leads to increased expression of FLOWERING LOCUS T. This, and analysis of ubp12 co mutants, indicates that UBP12 and UBP13 regulate photoperiodic flowering through a CO-dependent pathway. In addition, UBP12 and UBP13 regulate the circadian rhythm of clock genes, including LATE ELONGATED HYPOCOTYL, CIRCADIAN CLOCK ASSOCIATED1, and TIMING OF CAB EXPRESSION1. Furthermore, UBP12 and UBP13 are circadian controlled. Therefore, our work reveals a role for two deubiquitinases, UBP12 and UBP13, in the control of the circadian clock and photoperiodic flowering, which extends our understanding of ubiquitin in daylength measurement in higher plants.
authors: State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. xcui@genetics.ac.cn
authors: Xia
authors: X
authors: Cui
authors: Falong
authors: F
authors: Lu
authors: Yue
authors: Y
authors: Li
authors: Yongming
authors: Y
authors: Xue
authors: Yanyuan
authors: Y
authors: Kang
authors: Shuaibin
authors: S
authors: Zhang
authors: Qi
authors: Q
authors: Qiu
authors: Xiekui
authors: X
authors: Cui
authors: Shuzhi
authors: S
authors: Zheng
authors: Bin
authors: B
authors: Liu
authors: Xiaodong
authors: X
authors: Xu
authors: Xiaofeng
authors: X
authors: Cao
mesh_terms: D017360:Arabidopsis / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D057906:Circadian Clocks / Q000235:genetics*; D002940:Circadian Rhythm / Q000235:genetics; D003593:Cytoplasm / Q000378:metabolism; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism*; D035264:Flowers / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D009154:Mutation; D010641:Phenotype; D017440:Photoperiod; D030821:Plants, Genetically Modified; D017386:Sequence Homology, Amino Acid; D054875:Ubiquitination
chemical_list: D029681:Arabidopsis Proteins; D010450:Endopeptidases; C561399:UBP12 protein, Arabidopsis; C561400:UBP13 protein, Arabidopsis
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