title: Phosphatidylcholine dimers can be easily misinterpreted as cardiolipins in complex lipid mixtures: a matrix-assisted laser desorption/ionization time-of-flight mass spectrometric study of lipids from hepatocytes.
abstract: The liver is an important organ that is particularly involved in the lipid metabolism of the organism. Thus, high interest is nowadays focused on the lipid composition of the liver and particularly the liver parenchymal cells, the hepatocytes. Hepatocytes contain common phospholipids (PL) such as phosphatidylcholines, -ethanolamines and -inositols, for instance, that can be easily analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) even without previous separation of the PL mixture. However, in addition to common PL, hepatocytes possess also significant amounts of cardiolipin (CLP). The MS analysis of this PL is quite challenging because it (a) has a higher mass than common lipids and (b) possesses a higher negative charge. We will show here that caution is required if CLP is analyzed directly from the total lipid extract because PC dimers may be interpreted as cardiolipins if the positive ion MALDI mass spectra are analyzed.
authors: University of Leipzig, Faculty of Medicine, Institute of Medical Physics and Biophysics, Härtelstrasse 16-18, D-04107 Leipzig, Germany.
authors: Mandy
authors: M
authors: Eibisch
authors: Sebastian
authors: S
authors: Zellmer
authors: Rolf
authors: R
authors: Gebhardt
authors: Rosmarie
authors: R
authors: Süss
authors: Beate
authors: B
authors: Fuchs
authors: Jürgen
authors: J
authors: Schiller
mesh_terms: D000818:Animals; D002308:Cardiolipins / Q000737:chemistry* / Q000378:metabolism; D002478:Cells, Cultured; D045424:Complex Mixtures / Q000737:chemistry* / Q000378:metabolism; D019281:Dimerization; D022781:Hepatocytes / Q000737:chemistry* / Q000378:metabolism; D008055:Lipids / Q000737:chemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010713:Phosphatidylcholines / Q000737:chemistry* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D002308:Cardiolipins; D045424:Complex Mixtures; D008055:Lipids; D010713:Phosphatidylcholines