title: Resveratrol role in Staphylococcus aureus-induced corneal inflammation.
abstract: The aim of this study was to evaluate the role of trans-resveratrol on Staphylococcus aureus-induced keratitis. Rabbit corneas (intact corneas, abraded corneas and abraded corneas exposed to inactivated S. aureus strains) were placed in an ex vivo culture model. The abraded corneas exposed to S. aureus were divided into two 1-h-treatment sub-groups: corneas treated with trans-resveratrol and corneas treated with vehicle. The tissues were examined by immunohistochemical analyses and quantitative real-time RT-PCR to determine whether resveratrol could reduce TLR2-mediated recognition of S. aureus on epithelial cells and, if so, whether this reduction repressed the expression of inflammatory cytokines. The results demonstrated that resveratrol treatment effectively downregulated cell surface TLR2 on cells stimulated by S. aureus and reduced the expression of interleukin-8 gene. In addition, the corneal culture model tested, which is simple and reproducible, could be an alternative to in vivo animal testing for the development of novel specific therapies.
authors: Dipartimento di Scienze del Farmaco e dei Prodotti per la Salute, Università di Messina, Messina, Italy. anmarino@unime.it
authors: Andreana
authors: A
authors: Marino
authors: Giuseppe
authors: G
authors: Santoro
authors: Francesca
authors: F
authors: Spataro
authors: Eugenia R
authors: ER
authors: Lauriano
authors: Simona
authors: S
authors: Pergolizzi
authors: Francesco
authors: F
authors: Cimino
authors: Antonio
authors: A
authors: Speciale
authors: Antonia
authors: A
authors: Nostro
authors: Giuseppe
authors: G
authors: Bisignano
authors: Giacomo
authors: G
authors: Dugo
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage*; D003315:Cornea / Q000276:immunology / Q000473:pathology; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D007150:Immunohistochemistry; D016209:Interleukin-8 / Q000096:biosynthesis; D007634:Keratitis / Q000188:drug therapy* / Q000382:microbiology*; D011817:Rabbits; D060888:Real-Time Polymerase Chain Reaction; D000077185:Resveratrol; D013203:Staphylococcal Infections / Q000188:drug therapy* / Q000382:microbiology; D013267:Stilbenes / Q000008:administration & dosage*; D051195:Toll-Like Receptor 2 / Q000096:biosynthesis; D016896:Treatment Outcome
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D016209:Interleukin-8; D013267:Stilbenes; D051195:Toll-Like Receptor 2; D000077185:Resveratrol