title: [The functional changes in L-type Ca2+ channel of hypertrophied cardiomyocytes in neonatal rats induced by angiotensin II].
abstract: OBJECTIVE
  To investigate the molecular and functional changes in L-type Ca2+ channel of hypertrophied cardiomyocytes in neonatal rats induced by angiotensin II (Ang II).
  
  
  METHODS
  The in vitro model of cardiomyocyte hypertrophy was established in cultured cardiomyocytes from neonatal rats. Whole cell patch clamp was used to measure the L-type Ca2+ currents. Semi-quantitative RT-PCR was used to determine the mRNA expression of L-type Ca2+ channel alpha1C subunits.
  
  
  RESULTS
  In the hypertrophied cardiomyocytes induced by Ang II, I(Ca, L) densities were increased, whereas the features of I(Ca,L) activation, inactivation or recovery from inactivation were not affected. Meanwhile, Ang II increased the mRNA expression of L-type Ca2+ channel alpha1C subunits in cardiomyocytes. All these actions of Ang II could be blocked by the angiotensin II 1 type receptor blocker losartan.
  
  
  CONCLUSIONS
  During cardiomyocyte hypertrophy induced by Ang II, there are significant changes in the molecule and function of L-type Ca2+ channels, which are mediated by the angiotensin II 1 type receptor.
authors: Beijing Changping Hua Yi Hospital, Beijing 102208, China. YQL7291@163.com
authors: Qiu-Li
authors: QL
authors: Yan
authors: Wei
authors: W
authors: Hua
authors: Liang-Zhu
authors: LZ
authors: Yu
mesh_terms: D000804:Angiotensin II / Q000009:adverse effects*; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020746:Calcium Channels, L-Type / Q000378:metabolism*; D005260:Female; D006984:Hypertrophy / Q000378:metabolism; D008297:Male; D008564:Membrane Potentials; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000473:pathology* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D020746:Calcium Channels, L-Type; D000804:Angiotensin II; D002118:Calcium