title: Induction of caspase-8 and death receptors by a new dammarane skeleton from the dried fruits of Forsythia koreana.
abstract: A new naturally occurring compound based on the dammarane skeleton, i.e. cabralealactone 3-acetate-24-methyl ether, was isolated from the aqueous methanolic extract of Forsythia koreana fruits, along with eight known compounds: cabralealactone 3-acetate, ursolic acid, arctigenin, arctiin, phillyrin, rutin, caffeic acid, and rosmarinic acid. The identification of the isolated compounds was based on their spectral analysis including: HREI-MS, 1D and 2D NMR spectroscopy. The selected compounds and the aqueous methanolic extract were evaluated for their cytotoxic activity against human solid tumour cell lines. Cabralealactone 3-acetate-24-methyl ether and ursolic acid were found to be active against human breast cancer cells (MCF-7). The cytotoxicity was associated with the activation of caspase-8, the induction of the death receptors DR4 and DR5, as well as DNA fragmentation, and was thus due to apoptosis rather than necrosis.
authors: Phytochemistry and Plant Systematic Department, National Research Center, Dokki 12622, Cairo, Egypt. hawasusama@yahoo.com
authors: Usama W
authors: UW
authors: Hawas
authors: Amira M
authors: AM
authors: Gamal-Eldeen
authors: Samy K
authors: SK
authors: El-Desouky
authors: Young-Kyoon
authors: YK
authors: Kim
authors: Antje
authors: A
authors: Huefner
authors: Robert
authors: R
authors: Saf
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D053181:Caspase 8 / Q000096:biosynthesis*; D045744:Cell Line, Tumor; D004790:Enzyme Induction; D031657:Forsythia / Q000737:chemistry*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D053218:Receptors, Death Domain / Q000096:biosynthesis*; D021241:Spectrometry, Mass, Electrospray Ionization; D014315:Triterpenes / Q000737:chemistry / Q000494:pharmacology*; D000097669:Dammaranes
chemical_list: D053181:Caspase 8; D053218:Receptors, Death Domain; D014315:Triterpenes; D000097669:Dammaranes