title: 6-Acetonyl-5,6-dihydrosanguinarine (ADS) from Chelidonium majus L. triggers proinflammatory cytokine production via ROS-JNK/ERK-NFκB signaling pathway.
abstract: Chelidonium majus L. is an herbal plant that is commonly used in Western phytotherapy and traditional Chinese medicine for diuretic, antitussive, eye-regenerative, anti-osteoporotic, and radioprotective purposes. In this study, we purified 6-acetonyl-5,6-dihydrosanguinarine (ADS) from C. majus and investigated its immune-stimulatory effect. We found that ADS has the potential to induce the inflammatory cytokines TNF-α, IL-6, and IL-8 in macrophages and dendritic cells (DCs), that NFκB activation is a critical mediator of ADS-induced cytokine production, and that the activation of NFκB was dependent on reactive oxygen species (ROS). ADS induced phosphorylation of ERK and JNK, which was also associated with NFκB activation; phosphorylarion and cytokine production were inhibited by ROS scavenger and by specific MAPK inhibitors. Taken together, the results suggest that ADS from C. majus, as a positive immune modulator, induces inflammatory cytokines that might improve immunity, via the ROS-ERK/JNK-NFκB pathway.
authors: Division of Applied Life Science (BK21), PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors: Dong Hyeok
authors: DH
authors: Kim
authors: Ji Hye
authors: JH
authors: Lee
authors: Soojong
authors: S
authors: Park
authors: Sang-seok
authors: SS
authors: Oh
authors: Suk
authors: S
authors: Kim
authors: Dae Wook
authors: DW
authors: Kim
authors: Ki Hun
authors: KH
authors: Park
authors: Kwang Dong
authors: KD
authors: Kim
mesh_terms: D000818:Animals; D052117:Benzodioxoles / Q000494:pharmacology*; D053119:Benzophenanthridines / Q000494:pharmacology*; D002460:Cell Line; D028281:Chelidonium / Q000378:metabolism*; D016207:Cytokines / Q000096:biosynthesis*; D018836:Inflammation Mediators / Q000378:metabolism*; D020935:MAP Kinase Signaling System; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: C586708:6-acetonyl-5,6-dihydrosanguinarine; D052117:Benzodioxoles; D053119:Benzophenanthridines; D016207:Cytokines; D018836:Inflammation Mediators; D016328:NF-kappa B; D017382:Reactive Oxygen Species
keywords: 6-Acetonyl-5,6-dihydrosanguinarine; Chelidonium majus; Inflammatory cytokine; MAPK; NFκB; ROS