title: Plasma volume expansion by 0.9% NaCl during sepsis/systemic inflammatory response syndrome, after hemorrhage, and during a normal state.
abstract: OBJECTIVE
  The objective of this study was to determine the degree of plasma volume expansion by 0.9% NaCl in relation to the infused volume, in sepsis/systemic inflammatory response syndrome (SIRS), after a standardized hemorrhage, and in a normal condition.
  
  
  METHODS
  Prospective, randomized animal study.
  
  
  METHODS
  The study was performed at a university hospital laboratory.
  
  
  METHODS
  Thirty anesthetized adult male rats were included in the study.
  
  
  METHODS
  The study was performed in three groups: a sepsis/SIRS group (the S group), in which sepsis/SIRS was induced by cecal ligation and incision; a hemorrhage group (the H group), in which the rats were left without intervention for 4 h and bled 8 mL/kg thereafter; and a group that was left without intervention (the N group). Then, 4 h after baseline, all three groups were given an infusion of 0.9% NaCl (32 mL/kg) for 15 min. Baseline was defined as the time point when the surgical preparation was finished.
  
  
  RESULTS
  Plasma volumes were measured using I-albumin dilution technique at baseline, after 4 h, and 20 min after the end of infusion. The plasma volume-expanding effect 20 min after end of infusion was 0.6% ± 2.9% in the S group, 20% ± 6.4% in the H group, and 12% ± 11% in the N group, compared with just before start of infusion.
  
  
  CONCLUSIONS
  The present study in rats showed that the plasma volume-expanding effect after an infusion of 0.9% NaCl was smaller in a septic/SIRS state than after hemorrhage and in a normal state. This indicates that the plasma volume-expanding effect of a crystalloid is dependent on pathophysiologic changes in sepsis/SIRS.
authors: Department of Anesthesia and Intensive Care, Lund University and Lund University Hospital, Lund, Sweden. bjorn.bark@med.lu.se
authors: Björn P
authors: BP
authors: Bark
authors: Carl M
authors: CM
authors: Öberg
authors: Per-Olof
authors: PO
authors: Grände
mesh_terms: D000818:Animals; D006470:Hemorrhage / Q000503:physiopathology*; D008297:Male; D010952:Plasma Substitutes / Q000627:therapeutic use; D010953:Plasma Volume / Q000187:drug effects*; D051381:Rats; D012965:Sodium Chloride / Q000627:therapeutic use*; D018746:Systemic Inflammatory Response Syndrome / Q000188:drug therapy*
chemical_list: D010952:Plasma Substitutes; D012965:Sodium Chloride