title: Maternal engineered nanomaterial exposure and fetal microvascular function: does the Barker hypothesis apply?
abstract: OBJECTIVE
  The continued development and use of engineered nanomaterials (ENM) has given rise to concerns over the potential for human health effects. Although the understanding of cardiovascular ENM toxicity is improving, one of the most complex and acutely demanding "special" circulations is the enhanced maternal system to support fetal development. The Barker hypothesis proposes that fetal development within a hostile gestational environment may predispose/program future sensitivity. Therefore, the objective of this study was 2-fold: (1) to determine whether maternal ENM exposure alters uterine and/or fetal microvascular function and (2) test the Barker hypothesis at the microvascular level.
  
  
  METHODS
  Pregnant (gestation day 10) Sprague-Dawley rats were exposed to nano-titanium dioxide aerosols (11.3 ± 0.039 mg/m(3)/hr, 5 hr/d, 8.2 ± 0.85 days) to evaluate the maternal and fetal microvascular consequences of maternal exposure. Microvascular tissue isolation (gestation day 20) and arteriolar reactivity studies (<150 μm passive diameter) of the uterine premyometrial and fetal tail arteries were conducted.
  
  
  RESULTS
  ENM exposures led to significant maternal and fetal microvascular dysfunction, which was seen as robustly compromised endothelium-dependent and -independent reactivity to pharmacologic and mechanical stimuli. Isolated maternal uterine arteriolar reactivity was consistent with a metabolically impaired profile and hostile gestational environment that impacted fetal weight. The fetal microvessels that were isolated from exposed dams demonstrated significant impairments to signals of vasodilation specific to mechanistic signaling and shear stress.
  
  
  CONCLUSIONS
  To our knowledge, this is the first report to provide evidence that maternal ENM inhalation is capable of influencing fetal health and that the Barker hypothesis is applicable at the microvascular level.
authors: Center for Cardiovascular and Respiratory Sciences and Department of Physiology and Pharmacology, West Virginia University School of Medicine, Morgantown, WV 26506-9105, USA.
authors: Phoebe A
authors: PA
authors: Stapleton
authors: Valerie C
authors: VC
authors: Minarchick
authors: Jinghai
authors: J
authors: Yi
authors: Kevin
authors: K
authors: Engels
authors: Carroll R
authors: CR
authors: McBride
authors: Timothy R
authors: TR
authors: Nurkiewicz
mesh_terms: D000818:Animals; D004730:Endothelium, Vascular / Q000502:physiology; D005260:Female; D047109:Fetal Development / Q000187:drug effects; D005333:Fetus / Q000187:drug effects*; D018811:Maternal Exposure / Q000009:adverse effects*; D008833:Microcirculation / Q000187:drug effects; D049329:Nanostructures / Q000633:toxicity*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D014025:Titanium / Q000633:toxicity; D014590:Uterine Contraction / Q000187:drug effects; D014599:Uterus / Q000098:blood supply / Q000187:drug effects; D014664:Vasodilation / Q000187:drug effects
chemical_list: C009495:titanium dioxide; D014025:Titanium
keywords: Barker hypothesis; engineered nanomaterials (ENM); microvascular
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