title: Metabolic and molecular methods to evaluate the organoclay effects on a bacterial community.
abstract: The aim of this work was to evaluate the influence exerted by two different commercial organoclays (DELLITE 43B and DELLITE 67G) on a model microbial consortium using microbial metabolic characterization with BIOLOG system and denaturing gradient gel electrophoresis (DGGE) molecular approach. The information obtained from the molecular analyses, in their complex, account for the differences in species composition induced on the reference consortium by the contact with the organoclays under study. DELLITE 43B resulted to produce a marked selective effect, stimulating the quantitative increase especially of Pseudomonas pseudoalcaligenes. A weaker effect was found for DELLITE 67G. On the other hand, Biolog analyses indicated a depressing action exerted by DELLITE 43B on the metabolic activity of the model microbial consortium as a whole. The presence of P. pseudoalcaligenes and B. borstelensis in the bacterial community after the treatments confirmed that a positive change in the microbial structure consortium occurred.
authors: Dipartimento di Scienze delle Produzioni Agrarie e Alimentari, University of Catania, Via Santa Sofia 98, 95123 Catania, Italy. cristina.abbate@unict.it
authors: Cristina
authors: C
authors: Abbate
authors: Roberto
authors: R
authors: Ambrosoli
authors: Josè Luis
authors: JL
authors: Minati
authors: Mara
authors: M
authors: Gennari
authors: Maria
authors: M
authors: Arena
mesh_terms: D000538:Aluminum Silicates / Q000737:chemistry / Q000378:metabolism*; D001419:Bacteria / Q000254:growth & development / Q000378:metabolism*; D000077215:Clay; D017753:Ecosystem; D052918:Environmental Restoration and Remediation / Q000379:methods; D059013:Microbial Consortia / Q000502:physiology*; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000538:Aluminum Silicates; D012987:Soil; D012989:Soil Pollutants; D000077215:Clay