title: HPV-genotypes in high-grade intraepithelial cervical lesions in Danish women.
abstract: OBJECTIVE
  A study was undertaken to assess the distribution of high-risk HPV-genotypes in high-grade cervical intraepithelial neoplastic lesions in Danish women.
  
  
  METHODS
  Observational, cross-sectional.
  
  
  METHODS
  Danish data from a multi-centre study undertaken in 13 European countries.
  
  
  METHODS
  290 archived fixed biopsies with high-grade cervical lesions from the Departments of Pathology at the University Hospitals in Hvidovre and Odense, Denmark.
  
  
  METHODS
  Relevant histological samples were anonymized and shipped to a central laboratory for histopathology review and PCR-testing for HPV-DNA. A standardised HPV-test methodology was utilised to enable comparison of HPV-genotype distribution.
  
  
  RESULTS
  Of 290 Danish cervical samples, 276 were evaluated as histologically adequate and all of these were HPV-positive (HPV⁺). Of the HPV⁺ samples 77.9% were diagnosed with a single HPV-type, with cervical intraepithelial neoplasia (CIN)3 diagnosed in 82.3% and CIN2, CIN2/3, adenocarcinoma in situ (AIS) and AIS⁺ other high-grade lesion diagnosed in the remaining 17.7%. The most prevalent HPV-types were: HPV16 (54.0%), HPV33 (13.5%), HPV31 (10.7%), HPV18 (7.9%) and HPV52 (4.7%). Of the HPV⁺ samples, 21.4% were diagnosed with multiple HPV-types, with CIN3 diagnosed in 79.6% and CIN2, CIN2/3, AIS and AIS⁺ other high-grade lesion diagnosed in the remaining 20.4%. The most prevalent HPV-types were: HPV16 (49.2%), HPV31 (30.5%), HPV52 (27.1%), HPV51 (20.3%), HPV18 (16.9%), HPV33 (13.6%), HPV45 (11.9%), with 0.7% unknown types.
  
  
  CONCLUSIONS
  HPV16 and HPV18 were detected in approximately 75% of high-grade intraepithelial cervical lesions in a Danish population (single or multiple infections); these two genotypes are considered causative in at least 61.9% of the high-grade intraepithelial lesions (single infection).
authors: Department of Gynecology & Obstetrics, Hvidovre University Hospital, Copenhagen, Denmark. benny.kirschner@dadlnet.dk
authors: Benny
authors: B
authors: Kirschner
authors: Doris
authors: D
authors: Schledermann
authors: Katsiaryna
authors: K
authors: Holl
authors: Mats
authors: M
authors: Rosenlund
authors: Alice
authors: A
authors: Raillard
authors: Wim
authors: W
authors: Quint
authors: Anco
authors: A
authors: Molijn
authors: David
authors: D
authors: Jenkins
authors: Jette
authors: J
authors: Junge
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D052685:Alphapapillomavirus / Q000235:genetics*; D003430:Cross-Sectional Studies; D004279:DNA, Viral / Q000235:genetics*; D003718:Denmark; D005260:Female; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D002583:Uterine Cervical Neoplasms / Q000473:pathology / Q000821:virology*; D002578:Uterine Cervical Dysplasia / Q000473:pathology / Q000821:virology*
chemical_list: D004279:DNA, Viral
keywords: Cervical neoplasia; human papillomavirus; oncology; screening; vaccination