title: A universal vector for high-efficiency multi-fragment recombineering of BACs and knock-in constructs.
abstract: There is an increasing need for more efficient generation of transgenic constructs. Here we present a universal multi-site Gateway vector for use in recombineering reactions. Using transgenic mouse models, we show its use for the generation of BAC transgenics and targeting vectors. The modular nature of the vector allows for rapid modification of constructs to generate different versions of the same construct. As such it will help streamline the generation of series of related transgenic models.
authors: MRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine, University of Edinburgh, Western General Hospital, Edinburgh, United Kingdom.
authors: Karamjit Singh
authors: KS
authors: Dolt
authors: Melanie L
authors: ML
authors: Lawrence
authors: Eve
authors: E
authors: Miller-Hodges
authors: Joan
authors: J
authors: Slight
authors: Anna
authors: A
authors: Thornburn
authors: Paul S
authors: PS
authors: Devenney
authors: Peter
authors: P
authors: Hohenstein
mesh_terms: D000818:Animals; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D005260:Female; D055879:Gene Knock-In Techniques / Q000379:methods*; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D051379:Mice; D011995:Recombination, Genetic
references: EMBO J. 2006 Nov 1;25(21):5214-28
references: 17036046
references: Proc Natl Acad Sci U S A. 2011 Aug 23;108(34):14198-203
references: 21831835
references: J Biotechnol. 2004 Feb 5;107(3):233-43
references: 14736459
references: Nature. 2011 Jun 15;474(7351):337-42
references: 21677750
references: Nat Genet. 2010 Jan;42(1):89-93
references: 20023660
references: Dev Cell. 2010 May 18;18(5):698-712
references: 20493806
references: Nucleic Acids Res. 2007;35(8):e64
references: 17426124
references: Cell Stem Cell. 2008 Aug 7;3(2):169-81
references: 18682239
references: Nat Methods. 2008 May;5(5):409-15
references: 18391959
references: Cell. 2009 Aug 21;138(4):722-37
references: 19703398
references: Dis Model Mech. 2009 Sep-Oct;2(9-10):508-15
references: 19692579
references: Nat Biotechnol. 2011 Jul 07;29(8):731-4
references: 21738127
references: Nature. 2005 Jun 2;435(7042):646-51
references: 15806097
references: Nat Biotechnol. 2004 May;22(5):589-94
references: 15064769
references: Nat Rev Genet. 2001 Oct;2(10):769-79
references: 11584293
references: Eur J Immunol. 2007 Jan;37(1):43-53
references: 17171761
references: PLoS One. 2010 Jun 22;5(6):e11260
references: 20582321
references: Nat Methods. 2009 May;6(5):363-9
references: 19337237
references: Genome Res. 2003 Mar;13(3):476-84
references: 12618378
references: Nat Methods. 2007 Mar;4(3):251-6
references: 17293868
references: Transgenic Res. 2001 Apr;10(2):83-103
references: 11305364
references: Proc Natl Acad Sci U S A. 2007 Mar 13;104(11):4455-60
references: 17360545
references: Nucleic Acids Res. 2012 Apr;40(8):e55
references: 22241772
references: Development. 2011 Apr;138(7):1247-57
references: 21350016
references: Dev Cell. 2012 Sep 11;23(3):637-51
references: 22902740
references: Nature. 2007 Dec 20;450(7173):1230-4
references: 18097409
references: Nat Protoc. 2009;4(2):206-23
references: 19180090
references: Pathogenetics. 2008 Nov 03;1(1):3
references: 19014667