title: [Expression of gdnf and nos in adult zebrafish brain during the regeneration after spinal cord injury].
abstract: Recently, it is unclear about the mechanism of notable regenerated ability of adult zebrafish after spinal cord injury. To investigate the effects of brain on restoration from spinal cord injury, adult zebrafish spinal cord injury model was built and brain samples were dissected at different time points after the injury. Real-time quantitative PCR and in situ hybridization were applied to reveal the dynamics of glial cell line-derived neurotrophic factor (gdnf) and nitric oxide synthases (nos) mRNA expression in various regions of zebrafish brain. The results showed that, compared to sham group at each time points separately, the expression of gdnf mRNA in adult zebrafish brain during both acute phase (4 h and 12 h) and chronic phase of neuroregeneration (6 d and 11 d) increased significantly (P<0.05). The expression of nos mRNA in zebrafish brain enhanced during acute phase, and then reduced to the level lower than the sham group during the chronic phase of neuroregeneration (11 d) (P<0.05). This suggests that brain may promote neural axons regeneration in spinal cord via a more beneficial microenvironment which retains higher level of gdnf and lower level of nos.
authors: Center for Neuroscience, Shantou University Medical College, Shantou, China. xielin6@yahoo.cn
authors: Lin
authors: L
authors: Xie
authors: Ping
authors: P
authors: Fang
authors: Jin-Fei
authors: JF
authors: Lin
authors: Hong-Chao
authors: HC
authors: Pan
authors: Fan
authors: F
authors: Zhang
authors: Yan-Qin
authors: YQ
authors: Shen
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D051098:Glial Cell Line-Derived Neurotrophic Factors / Q000235:genetics*; D052248:Nitric Oxide Synthase Type I / Q000235:genetics*; D012038:Regeneration / Q000235:genetics*; D013119:Spinal Cord Injuries / Q000235:genetics / Q000473:pathology / Q000503:physiopathology*; D013997:Time Factors; D015854:Up-Regulation; D015027:Zebrafish / Q000235:genetics* / Q000502:physiology
chemical_list: D051098:Glial Cell Line-Derived Neurotrophic Factors; D052248:Nitric Oxide Synthase Type I