title: Stimulatory effect of gonadal hormones on fetal rat hippocampal neural proliferation requires neurotrophin receptor activation in vitro.
abstract: To determine the effects of gonadal hormones on proliferation of the hippocampal neural cells, which are of importance in learning and memory function. 17β-Estradiol or testosterone was added to the culture at various concentrations. Their proliferation and protective effects on the neural cell were determined with BrdU, flow cytometry and MTT assay. Effects of the gonadal hormones on brain-derived neurotrophic factor (BDNF) expression were determined using ELISA and RT-PCR respectively. 17β-Estradiol and testosterone at 20nM or higher concentrations significantly increased the neural cell proliferation and viability, and induced increasing in the S phase arrest which is essential for cell proliferation. Both estradiol and testosterone significantly increased the neural cell expression of cellular mature BDNF and BDNF mRNA. Effect of testosterone on hippocampal neural proliferation was blocked by Trk neurotrophin receptor inhibitor. 17β-Estradiol and testosterone promoted hippocampal neural proliferation and improved cell viability in vitro. The effect of testosterone on hippocampal neural cell proliferation required neurotrophin receptor activation.
authors: Department of Nutrition, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China. meixpan@yahoo.com.cn
authors: Meixia
authors: M
authors: Pan
authors: Caixia
authors: C
authors: Zhang
mesh_terms: D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000008:administration & dosage*; D042341:Gonadal Hormones / Q000008:administration & dosage; D006624:Hippocampus / Q000187:drug effects / Q000196:embryology* / Q000502:physiology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D051381:Rats; D017475:Receptors, Nerve Growth Factor / Q000378:metabolism*; D013739:Testosterone / Q000008:administration & dosage*
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D042341:Gonadal Hormones; D017475:Receptors, Nerve Growth Factor; D013739:Testosterone; D004958:Estradiol