title: Interaction of the Capsular Polysaccharide A from Bacteroides fragilis with DC-SIGN on Human Dendritic Cells is Necessary for Its Processing and Presentation to T Cells.
abstract: The zwitterionic capsular polysaccharide A (PSA) of Bacteroides fragilis is the first carbohydrate antigen described to be presented in major histocompatibility complex (MHC) class II for the induction of CD4(+) T cell responses. However, the identity of the receptor mediating binding and internalization of PSA in antigen presenting cells remains elusive. C-type lectins are glycan-binding receptors known for their capacity to target ligands for antigen presentation to T cells. Here, we investigated whether C-type lectins were involved in the internalization of PSA and identified dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN) as the main receptor for PSA on human dendritic cells (DC). The induction of PSA-specific T cell proliferation appeared to be completely dependent on DC-SIGN. These data reveal a crucial role for DC-SIGN in the endocytosis and routing of PSA in human DC for the efficient stimulation of PSA-specific CD4(+) T cells.
authors: Department of Molecular Cell Biology and Immunology, VU University Medical Center Amsterdam, Netherlands ; Centre for Specialized Nutrition, Danone Research Wageningen, Netherlands.
authors: Karien
authors: K
authors: Bloem
authors: Juan J
authors: JJ
authors: García-Vallejo
authors: Ilona M
authors: IM
authors: Vuist
authors: Brian A
authors: BA
authors: Cobb
authors: Sandra J
authors: SJ
authors: van Vliet
authors: Yvette
authors: Y
authors: van Kooyk
keywords: C-type lectin; T cell proliferation; dendritic cell; pathogen; polysaccharide
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