title: MicroRNA-376c inhibits cell proliferation and invasion in osteosarcoma by targeting to transforming growth factor-alpha.
abstract: MicroRNAs are a class of small noncoding RNAs that function as critical gene regulators through targeting mRNAs for translational repression or degradation. In this study, we showed that miR-376c expression level was decreased while transforming growth factor-alpha (TGFA) mRNA expression levels were increased in osteosarcoma tissues and cell lines, and we identified TGFA as a novel direct target of miR-376c. Overexpression of miR-376c suppressed TGFA expression and the expression of its downstream signaling molecule such as epidermal growth factor receptor, and attenuated cell proliferation and invasion. Forced expression of TGFA could partly rescue the inhibitory effect of miR-376c in the cells. Taken together, these findings will shed light on the role and mechanism of miR-376c in regulating osteosarcoma cell growth via miR-376c/TGFA axis, and miR-376c may serve as a potential therapeutic target in osteosarcoma in the future.
authors: Department of Orthopedics, Second Xiangya Hospital, Central South University, Changsha, China.
authors: Yi
authors: Y
authors: Jin
authors: Dan
authors: D
authors: Peng
authors: Yi
authors: Y
authors: Shen
authors: Min
authors: M
authors: Xu
authors: Ying
authors: Y
authors: Liang
authors: Bowen
authors: B
authors: Xiao
authors: Jing
authors: J
authors: Lu
mesh_terms: D020413:3' Untranslated Regions; D001665:Binding Sites; D001859:Bone Neoplasms / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D015536:Down-Regulation; D066246:ErbB Receptors / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D012516:Osteosarcoma / Q000235:genetics* / Q000473:pathology*; D016211:Transforming Growth Factor alpha / Q000235:genetics* / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; C573498:MIRN376C microRNA, human; D035683:MicroRNAs; D016211:Transforming Growth Factor alpha; D066246:ErbB Receptors