title: Tips for preparing mRNA-Seq libraries from poly(A)+ mRNA for Illumina transcriptome high-throughput sequencing.
abstract: Many investigators who do high-throughput sequencing of mRNA (mRNA-Seq) use kits for library preparation purchased from Illumina. Recognizing that these kits are continually being updated and improved, we provide here some background information, tips, and troubleshooting advice for the kits available at the time of this writing.
authors: Brenton R
authors: BR
authors: Graveley
mesh_terms: D015723:Gene Library; D057166:High-Throughput Screening Assays / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics* / Q000302:isolation & purification; D013048:Specimen Handling / Q000379:methods*; D059467:Transcriptome
chemical_list: D012333:RNA, Messenger