title: A feasibility study on the prediction of acute graft-vs.-host disease before hematopoietic stem cell transplantation based on fetomaternal tolerance.
abstract: The contact between the immune systems of mother and child during pregnancy affects an immune response of the child against noninherited maternal antigens (NIMA) and the mother against inherited paternal antigens (IPA). However, the immunologic effects of developmental exposure to NIMA or IPA are heterogeneous, and can be either tolerogenic or immunogenic. Although we have reported that prediction of acute graft-vs.-host disease (GVHD) is feasible in a murine model, there has been no literature in human. We devised a novel method for predicting a tolerogenic effect by using mixed lymphocyte reaction combined with enzyme-linked immunospot (MLR-ELISPOT) assay. The assay can evaluate reactivity of interferon-γ spot-forming cells of donor against the recipient. Although we have shown only two examples of mother to child reactivity so far, our preliminary results suggest that this pre-screened assay may be used to predict acute GVHD. The clinical trial is in progress to evaluate MLR-ELISPOT assay as a predicting measure of acute GVHD in haploidentical transplantation from NIMA or IPA-mismatched family donor.
authors: Department of Pediatrics and Cell Transplantation; Mie University Graduate School of Medicine; Edobashi, Japan.
authors: Masahiro
authors: M
authors: Hirayama
authors: Eiichi
authors: E
authors: Azuma
authors: Tsuyoshi
authors: T
authors: Ito
authors: Yoshitaka
authors: Y
authors: Keida
authors: Yoshihiro
authors: Y
authors: Komada
mesh_terms: D000293:Adolescent; D000818:Animals; D002648:Child; D002986:Clinical Trials as Topic; D058501:Enzyme-Linked Immunospot Assay; D005240:Feasibility Studies; D005260:Female; D006086:Graft vs Host Disease / Q000175:diagnosis* / Q000276:immunology*; D006680:HLA Antigens / Q000032:analysis*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007108:Immune Tolerance; D007959:Lymphocyte Culture Test, Mixed; D008297:Male; D008431:Maternal-Fetal Exchange / Q000276:immunology; D051379:Mice; D011247:Pregnancy
chemical_list: D006680:HLA Antigens
keywords: MLR-ELISPOT; NIMA; acute GVHD; fetomaternal tolerance; hematopoietic stem cell transplantation
references: Blood. 2009 Feb 19;113(8):1829-33
references: 19124834
references: Chimerism. 2011 Jan;2(1):23-4
references: 21547034
references: Science. 1988 Sep 30;241(4874):1815-7
references: 3051377
references: Blood. 2002 Mar 1;99(5):1572-7
references: 11861270
references: Transplantation. 2009 Dec 15;88(11):1303-11
references: 19996930
references: Bone Marrow Transplant. 1999 Dec;24(11):1161-6
references: 10642803
references: Biol Reprod. 2005 Feb;72(2):460-9
references: 15469995
references: Ann Hematol. 1996 Apr;72(4):260-4
references: 8624381
references: Hum Immunol. 2000 Jun;61(6):548-54
references: 10825582
references: Front Immunol. 2012 May 25;3:135
references: 22654885
references: Hum Immunol. 2004 Mar;65(3):231-9
references: 15041161
references: Blood. 2004 Dec 1;104(12):3821-8
references: 15280193
references: Eur J Immunol. 1996 Apr;26(4):834-8
references: 8625975
references: Bone Marrow Transplant. 2001 Aug;28(4):375-80
references: 11571510
references: Bone Marrow Transplant. 2009 Sep;44(5):327-9
references: 19219076
references: J Clin Invest. 2008 Nov;118(11):3619-28
references: 18846251
references: J Immunol. 2003 Mar 1;170(5):2316-22
references: 12594253
references: J Immunol. 2010 Dec 15;185(12):7739-45
references: 21078914
references: Ann Hematol. 2004 Sep;83(9):566-72
references: 15170521
references: N Engl J Med. 1998 Dec 3;339(23):1657-64
references: 9834302
references: Eur J Haematol. 2007 Feb;78(2):157-60
references: 17313562