title: Elicitation of galanthamine biosynthesis by Leucojum aestivum liquid shoot cultures.
abstract: The effects of methyl jasmonate and jasmonic acid on galanthamine production, phenolic acid content and growth of Leucojum aestivum L. shoot culture, cultivated in submerged conditions were investigated. The best time-point for addition of elicitors was during the exponential phase of the culture growth. The maximal contents of galanthamine and lycorine (226.9 μg/flask and 491.4 μg/flask, 1.36 and 1.67-fold higher compared to the control, respectively) were achieved after elicitation with jasmonic acid, whereas the elicitation with methyl jasmonte resulted in maximal accumulation of phenolic acids. It was demonstrated that the boosting effect of jasmonic acid on Amaryllidacea alkaloid biosynthesis was due to induction of the activity of tyrosine decarboxylase, whereas methyl jasmonate stimulates the biosynthesis of phenolic acids by inducing mainly the activity of phenylalanine ammonia-lyase.
authors: Laboratory of Applied Biotechnologies, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 139 Ruski Boulevard, Plovdiv 4000, Bulgaria.
authors: Ivan
authors: I
authors: Ivanov
authors: Vasil
authors: V
authors: Georgiev
authors: Atanas
authors: A
authors: Pavlov
mesh_terms: D000085:Acetates / Q000494:pharmacology; D003517:Cyclopentanes / Q000494:pharmacology; D005702:Galantamine / Q000096:biosynthesis*; D019668:Liliaceae / Q000187:drug effects* / Q000378:metabolism*; D054883:Oxylipins / Q000494:pharmacology; D010650:Phenylalanine Ammonia-Lyase / Q000378:metabolism; D018520:Plant Shoots / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D005702:Galantamine; C011006:jasmonic acid; C072239:methyl jasmonate; D010650:Phenylalanine Ammonia-Lyase