title: Identification of acyl-CoA thioesterase in mouse mesenteric lymph nodes.
abstract: Acyl-CoA thioesterases (ACOTs) are a group of enzymes that catalyze the hydrolysis of fatty acyl-CoAs to free fatty acids and CoA, with the potential to regulate the intracellular levels of these molecules. In this study, we show that a cytosolic isoform, ACOT7, is expressed at a significant level in the mesenteric lymph nodes (MLNs) of mice. While crude preparations of the mesenteric visceral fat contained significant levels of palmitoyl-CoA thioesterase activity, enzyme activity was concentrated 6.9-fold in MLNs compared with the residual adipose portion after excision of MLNs. When MLN homogenates were centrifuged, 82% of the enzyme activity was recovered in the cytosolic fraction, concomitant with almost exclusive recovery of ACOT7. Immunoprecipitation using anti-ACOT7 antibody estimated that 87% of enzyme activity in the homogenates was accounted for by ACOT7. On MLN sections, the germinal centers of secondary lymphoid follicles were immunostained with the antibody. In MLNs of mice fasted for 16 h, ACOT7 levels were induced 1.8-fold, which reflected a 1.5-fold increase in enzyme activity. These findings suggest that ACOT7 may be involved in dietary intake-associated responses in fatty acid metabolism in MLNs.
authors: Department of Pharmacotherapeutics, Tokyo University of Pharmacy and Life Sciences, Tokyo 192–0392, Japan.
authors: Takayuki
authors: T
authors: Ohtomo
authors: Chisato
authors: C
authors: Nakao
authors: Megumi
authors: M
authors: Sumiya
authors: Osamu
authors: O
authors: Kaminuma
authors: Akemi
authors: A
authors: Abe
authors: Akio
authors: A
authors: Mori
authors: Niro
authors: N
authors: Inaba
authors: Tetsuta
authors: T
authors: Kato
authors: Junji
authors: J
authors: Yamada
mesh_terms: D000818:Animals; D008198:Lymph Nodes / Q000378:metabolism*; D008297:Male; D008643:Mesentery / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D010170:Palmitoyl-CoA Hydrolase / Q000378:metabolism*
chemical_list: C521939:Acot7 protein, mouse; D010170:Palmitoyl-CoA Hydrolase