title: The pentatricopeptide repeat MTSF1 protein stabilizes the nad4 mRNA in Arabidopsis mitochondria.
abstract: Gene expression in plant mitochondria involves a complex collaboration of transcription initiation and termination, as well as subsequent mRNA processing to produce mature mRNAs. In this study, we describe the function of the Arabidopsis mitochondrial stability factor 1 (MTSF1) gene and show that it encodes a pentatricopeptide repeat protein essential for the 3'-processing of mitochondrial nad4 mRNA and its stability. The nad4 mRNA is highly destabilized in Arabidopsis mtsf1 mutant plants, which consequently accumulates low amounts of a truncated form of respiratory complex I. Biochemical and genetic analyses demonstrated that MTSF1 binds with high affinity to the last 20 nucleotides of nad4 mRNA. Our data support a model for MTSF1 functioning in which its association with the last nucleotides of the nad4 3' untranslated region stabilizes nad4 mRNA. Additionally, strict conservation of the MTSF1-binding sites strongly suggests that the protective function of MTSF1 on nad4 mRNA is conserved in dicots. These results demonstrate that the mRNA stabilization process initially identified in plastids, whereby proteins bound to RNA extremities constitute barriers to exoribonuclease progression occur in plant mitochondria to protect and concomitantly define the 3' end of mature mitochondrial mRNAs. Our study also reveals that short RNA molecules corresponding to pentatricopeptide repeat-binding sites accumulate also in plant mitochondria.
authors: INRA, UMR1318, Institut Jean-Pierre Bourgin, RD10, F-78000 Versailles, France, AgroParisTech, Institut Jean-Pierre Bourgin, RD10, F-78000 Versailles, France.
authors: Nawel
authors: N
authors: Haïli
authors: Nadège
authors: N
authors: Arnal
authors: Martine
authors: M
authors: Quadrado
authors: Souad
authors: S
authors: Amiar
authors: Guillaume
authors: G
authors: Tcherkez
authors: Jennifer
authors: J
authors: Dahan
authors: Pierre
authors: P
authors: Briozzo
authors: Catherine
authors: C
authors: Colas des Francs-Small
authors: Nathalie
authors: N
authors: Vrielynck
authors: Hakim
authors: H
authors: Mireau
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001665:Binding Sites; D019069:Cell Respiration; D042967:Electron Transport Complex I / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D010788:Photosynthesis; D026721:RNA 3' End Processing; D012326:RNA Splicing; D020871:RNA Stability; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; C583509:MTSF1 protein, Arabidopsis; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C513204:NAD4 protein, Arabidopsis; D042967:Electron Transport Complex I
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