title: Identification of cellular factors required for the budding of koala retrovirus.
abstract: Koala retrovirus (KoRV) is a unique gammaretrovirus that is currently endogenizing into its host and considered to be associated with leukemia, lymphoma and immunosuppression in koalas (Phascolactos cinereus). In this study, it was demonstrated that WWP2 or WWP2-like E3 ubiquitin ligases possessing the WW domain closely related to WWP2 and Vps4A/B are involved in KoRV budding. These data suggest that KoRV Gag recruits the cellular endosomal sorting complex required for transport machinery through interaction of the PPPY L-domain with the WW domain(s) of WWP2 and that progeny virions are released from cells by utilizing the multivesicular body sorting pathway.
authors: Laboratory of Signal Transduction, Department of Cell Biology, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto 606-8507, Japan.
authors: Sayumi
authors: S
authors: Shimode
authors: Rie
authors: R
authors: Nakaoka
authors: Shigeki
authors: S
authors: Hoshino
authors: Masumi
authors: M
authors: Abe
authors: Hiroko
authors: H
authors: Shogen
authors: Jiro
authors: J
authors: Yasuda
authors: Takayuki
authors: T
authors: Miyazawa
mesh_terms: D000818:Animals; D002460:Cell Line; D017988:Gammaretrovirus / Q000502:physiology*; D015683:Gene Products, gag / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D006801:Humans; D048368:Phascolarctidae; D011485:Protein Binding; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism*; D057074:Virus Release
chemical_list: D015683:Gene Products, gag; C465283:WWP2 protein, human; D044767:Ubiquitin-Protein Ligases
keywords: budding; host factors; koala retrovirus; koalas