title: Proteomic characterization of inbreeding-related cold sensitivity in Drosophila melanogaster.
abstract: Inbreeding depression is a widespread phenomenon of central importance to agriculture, medicine, conservation biology and evolutionary biology. Although the population genetic principles of inbreeding depression are well understood, we know little about its functional genomic causes. To provide insight into the molecular interplay between intrinsic stress responses, inbreeding depression and temperature tolerance, we performed a proteomic characterization of a well-defined conditional inbreeding effect in a single line of Drosophila melanogaster, which suffers from extreme cold sensitivity and lethality. We identified 48 differentially expressed proteins in a conditional lethal line as compared to two control lines. These proteins were enriched for proteins involved in hexose metabolism, in particular pyruvate metabolism, and many were found to be associated with lipid particles. These processes can be linked to known cold tolerance mechanisms, such as the production of cryoprotectants, membrane remodeling and the build-up of energy reserves. We checked mRNA-expression of seven genes with large differential protein expression. Although protein expression poorly correlated with gene expression, we found a single gene (CG18067) that, after cold shock, was upregulated in the conditional lethal line both at the mRNA and protein level. Expression of CG18067 also increased in control flies after cold shock, and has previously been linked to cold exposure and chill coma recovery time. Many differentially expressed proteins in our study appear to be involved in cold tolerance in non-inbred individuals. This suggest the conditional inbreeding effect to be caused by misregulation of physiological cold tolerance mechanisms.
authors: Ecology and Genetics, Department of Biological Sciences, Aarhus University, Aarhus, Denmark. C.J.Vermeulen@rug.nl
authors: Cornelis J
authors: CJ
authors: Vermeulen
authors: Kamilla S
authors: KS
authors: Pedersen
authors: Hans C
authors: HC
authors: Beck
authors: Jørgen
authors: J
authors: Petersen
authors: Kristina Kirilova
authors: KK
authors: Gagalova
authors: Volker
authors: V
authors: Loeschcke
mesh_terms: D000818:Animals; D003080:Cold Temperature; D058639:Cold-Shock Response / Q000235:genetics; D004331:Drosophila melanogaster / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D005260:Female; D063990:Gene Ontology; D005943:Gluconeogenesis / Q000235:genetics; D006601:Hexoses / Q000378:metabolism; D007178:Inbreeding; D019476:Insect Proteins / Q000378:metabolism; D008297:Male; D025341:Principal Component Analysis; D040901:Proteomics; D059467:Transcriptome
chemical_list: D006601:Hexoses; D019476:Insect Proteins
references: Conserv Biol. 2009 Aug;23(4):920-30
references: 19627320
references: Nat Protoc. 2009;4(1):44-57
references: 19131956
references: Philos Trans R Soc Lond B Biol Sci. 2010 Jan 12;365(1537):147-54
references: 20008392
references: Annu Rev Physiol. 2005;67:225-57
references: 15709958
references: Bioinformatics. 2008 Dec 15;24(24):2894-900
references: 18974169
references: Electrophoresis. 2004 May;25(9):1327-33
references: 15174055
references: Heredity (Edinb). 2005 Sep;95(3):235-42
references: 16077737
references: Proc Natl Acad Sci U S A. 2007 Apr 17;104(16):6504-10
references: 17409186
references: Nucleic Acids Res. 2009 Jan;37(Database issue):D555-9
references: 18948289
references: Proteomics. 2004 May;4(5):1372-81
references: 15188405
references: J Insect Physiol. 2005 Nov;51(11):1173-82
references: 16112133
references: Genetics. 2008 Oct;180(2):1233-43
references: 18791253
references: Nucleic Acids Res. 2009 Apr;37(6):e45
references: 19237396
references: Genet Res. 1999 Dec;74(3):329-40
references: 10689809
references: Bioinformatics. 2001 Jun;17(6):520-5
references: 11395428
references: Ann N Y Acad Sci. 2012 May;1256:33-48
references: 22583046
references: Trends Genet. 2010 Apr;26(4):177-87
references: 20227782
references: Genetics. 2012 Sep;192(1):161-72
references: 22714404
references: Trends Ecol Evol. 2010 Jan;25(1):44-52
references: 19733933
references: Nat Rev Genet. 2009 Nov;10(11):783-96
references: 19834483
references: J Evol Biol. 2008 Sep;21(5):1236-44
references: 18631212
references: J Insect Physiol. 2009 Jun;55(6):549-55
references: 19232407
references: Genome Biol. 2003;4(5):P3
references: 12734009
references: Genetics. 2004 Jul;167(3):1241-8
references: 15280238
references: Philos Trans R Soc Lond B Biol Sci. 2010 Apr 27;365(1544):1229-39
references: 20308098
references: Physiol Genomics. 2012 Aug 1;44(15):764-77
references: 22735925
references: J Evol Biol. 2007 Jul;20(4):1624-36
references: 17584255
references: Proc Biol Sci. 2008 Sep 7;275(1646):2055-61
references: 18522910
references: J Insect Physiol. 2007 Dec;53(12):1218-32
references: 17662301
references: PLoS One. 2011 Jan 25;6(1):e15644
references: 21283582
references: Proteomics. 2006 Mar;6(6):1712-5
references: 16429464
references: Bioinformatics. 2008 Jul 1;24(13):1549-51
references: 18482995
references: Heredity (Edinb). 2004 Apr;92(4):275-81
references: 14679396
references: Evolution. 2011 Jan;65(1):246-58
references: 20731715
references: Insect Biochem Mol Biol. 2007 Nov;37(11):1177-88
references: 17916504
references: Genetics. 2006 Jul;173(3):1329-36
references: 16624914
references: Cell Mol Life Sci. 2009 Apr;66(8):1404-18
references: 19129970
references: Stat Appl Genet Mol Biol. 2004;3:Article3
references: 16646809
references: J Exp Biol. 2011 Dec 1;214(Pt 23):4021-9
references: 22071194
references: Genetics. 2005 Sep;171(1):157-67
references: 15944359
references: Insect Mol Biol. 2008 Sep;17(5):565-72
references: 18828842
references: Mol Cell Proteomics. 2006 Jun;5(6):1082-94
references: 16543254
references: Heredity (Edinb). 2010 Feb;104(2):125-34
references: 19812620
references: Neurosci Lett. 2003 Mar 13;339(1):62-6
references: 12618301