title: Expression of matrix metalloproteinase-12 in aortic dissection.
abstract: BACKGROUND
  Aortic dissection(AD) is an acute process of large blood vessels characterized by dangerous pathogenic conditions and high disability and high mortality. The pathogenesis of AD remains debated. Matrix metalloproteinase-12 (MMP-12) participates in many pathological processes such as abdominal aortic aneurysm, atherosclerosis, emphysema and cancer. However, this elastase has rarely been assessed in the presence of AD. The aim of the present study was to investigate the expression of MMP-12 in aortic tissue so as to offer a better understanding of the possible mechanisms of AD.
  
  
  METHODS
  The protein expression levels of MMP-12 were analyzed and compared in aorta tissue and the blood serum samples by reverse transcription polymerase chain reaction(RT-PCR), Western blotting, immuno-histochemistry, fluorescence resonance energy transfer ( FRET ) activity assay and enzyme-linked immuno sorbent assay ( ELISA ), respectively. Ascending aorta tissue specimens were obtained from 12 patients with an acute Stanford A-dissection at the time of aortic replacement, and from 4 patients with coronary artery disease (CAD) undergoing coronary artery bypass surgery. Meanwhile, serum samples were harvested from 15 patients with an acute Stanford A-dissection and 10 healthy individuals who served as the control group.
  
  
  RESULTS
  MMP-12 activity could be detected in both AD and CAD groups, but the level in the AD group was higher than those in the CAD group (P < 0.05). MMP-12 proteolysis existed in both serum samples of the AD and healthy groups, and the activity level in the AD group was clearly higher than in the healthy group (P < 0.05). For AD patients, MMP-12 activity in serum was higher than in the aorta wall (P < 0.05). MMP-12 activity in the aortic wall tissue can be inhibited by MMP inhibitor v (P < 0.05).
  
  
  CONCLUSIONS
  The present study directly demonstrates that MMP-12 proteolytic activity exists within the aorta specimens and blood samples from aortic dissection patients. MMP-12 might be of potential relevance as a clinically diagnostic tool and therapeutic target in vascular injury and repair.
authors: Laboratory of Molecular Cardiology, Department of Cardiology; The First Affiliated Hospital Of Kunming Medical University, Kunming, 650032, China.
authors: Yi
authors: Y
authors: Song
authors: Yuehui
authors: Y
authors: Xie
authors: Feng
authors: F
authors: Liu
authors: Chong
authors: C
authors: Zhao
authors: Rui
authors: R
authors: Yu
authors: Shao
authors: S
authors: Ban
authors: Qiufang
authors: Q
authors: Ye
authors: Jianxion
authors: J
authors: Wen
authors: Haibo
authors: H
authors: Wan
authors: Xiang
authors: X
authors: Li
authors: Runwei
authors: R
authors: Ma
authors: Zhaohui
authors: Z
authors: Meng
mesh_terms: D000328:Adult; D000368:Aged; D000784:Aortic Dissection / Q000175:diagnosis / Q000201:enzymology* / Q000235:genetics*; D001014:Aortic Aneurysm / Q000175:diagnosis / Q000201:enzymology* / Q000235:genetics*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D008297:Male; D053508:Matrix Metalloproteinase 12 / Q000096:biosynthesis / Q000235:genetics*; D008875:Middle Aged; D059748:Proteolysis
chemical_list: C507122:MMP12 protein, human; D053508:Matrix Metalloproteinase 12
references: Surg Today. 2004;34(3):220-5
references: 14999533
references: Circulation. 2010 Apr 6;121(13):e266-369
references: 20233780
references: J Biol Chem. 1993 Nov 15;268(32):23824-9
references: 8226919
references: J Surg Res. 1995 Jul;59(1):17-22
references: 7630123
references: Crit Care. 2013 Feb 25;17(1):R33
references: 23442769
references: Mol Aspects Med. 2008 Oct;29(5):329-38
references: 18657570
references: J Biol Chem. 1999 Apr 9;274(15):10019-23
references: 10187779
references: J Vasc Surg. 2012 Dec;56(6):1698-709, 1709.e1
references: 22960022
references: J Am Heart Assoc. 2012 Dec;1(6):e001040
references: 23316311
references: Biomed Pharmacother. 2003 Jul-Aug;57(5-6):195-202
references: 12888254
references: J Vasc Surg. 2007 Apr;45(4):849-57
references: 17398401
references: Am J Cardiol. 2012 Oct 1;110(7):1056-61
references: 22762721
references: Stroke. 2004 Jun;35(6):1310-5
references: 15073384
references: Natl Vital Stat Rep. 2008 Apr 24;56(10):1-120
references: 18512336
references: J Clin Invest. 1998 Dec 1;102(11):1900-10
references: 9835614
references: J Transl Med. 2006 Jul 06;4:29
references: 16824202
references: Am J Pathol. 1998 Mar;152(3):703-10
references: 9502412
references: Circulation. 2012 Dec 18;126(25):3070-80
references: 23136157
references: Circulation. 1997 Jan 7;95(1):205-12
references: 8994438
references: J Vasc Surg. 2002 Jul;36(1):1-12
references: 12096249
references: Curr Med Chem. 2008;15(14):1388-95
references: 18537616
references: J Biol Chem. 1997 May 2;272(18):12189-94
references: 9115292
references: Virchows Arch. 2006 Jun;448(6):811-21
references: 16609908
references: Biomark Insights. 2009 May 06;4:81-90
references: 19652764
references: Circ J. 2008 Nov;72(11):1773-6
references: 18832775
references: N Engl J Med. 2009 Dec 31;361(27):2599-608
references: 20018959
references: Circ Res. 1995 Nov;77(5):863-8
references: 7554139
references: J Mol Biol. 2001 Sep 28;312(4):743-51
references: 11575929
references: Circ Res. 2003 May 2;92(8):827-39
references: 12730128
references: Curr Pharm Des. 2007;13(20):2087-100
references: 17627541
references: J Surg Res. 2010 Oct;163(2):e99-104
references: 20708200
references: Biochem J. 1981 Feb 1;193(2):589-605
references: 7030312
references: Hum Pathol. 2000 Jun;31(6):640-6
references: 10872655
references: Circ Res. 2002 Feb 22;90(3):251-62
references: 11861412