title: A new electrochemically active bacterium phylogenetically related to Tolumonas osonensis and power performance in MFCs.
abstract: A facultative anaerobic bacterium (designated as P2-A-1) was isolated from microbial fuel cells (MFCs) inoculated with sludge from a sewage treatment plant. Based on 16S rDNA sequence analysis, the strain was identified as Tolumonas osonensis OCF 7(T) according to its biochemical, physiological and morphological characteristics. Through parameters optimization, the P2-A-1 MFC reached the maximum power density of 424 mW/m(2) in the substrate of 2g/L sodium acetate. Further, a facile bacteria treatment approach by chemically "perforating" pores and channels on bacterial membrane was developed to significantly improve the power density. And 1mM of EDTA-treated cell yielded the highest power density of 509.1 mW/m(2) because the membrane permeability of cell was enhanced by verification of coenzyme Q and fatty acid composition tests. It offers a novel facultative anaerobic Gram-positive bacterium that can utilize a wide variety of substrates for power production, making it highly valuable for application in MFCs.
authors: Key Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science & Technology), Ministry of Education, Tianjin Key Lab of Industrial Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, PR China. tjluojm@126.com
authors: Jianmei
authors: J
authors: Luo
authors: Jia
authors: J
authors: Yang
authors: Huanhuan
authors: H
authors: He
authors: Tao
authors: T
authors: Jin
authors: Li
authors: L
authors: Zhou
authors: Min
authors: M
authors: Wang
authors: Minghua
authors: M
authors: Zhou
mesh_terms: D015057:1-Naphthylamine / Q000031:analogs & derivatives / Q000378:metabolism; D044044:Aeromonadaceae / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D001674:Bioelectric Energy Sources / Q000382:microbiology*; D004560:Electricity; D004563:Electrochemistry; D004566:Electrodes; D004579:Electron Transport / Q000187:drug effects; D005227:Fatty Acids / Q000378:metabolism; D005453:Fluorescence; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D010802:Phylogeny; D019346:Sodium Acetate / Q000494:pharmacology; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D014451:Ubiquinone; D019346:Sodium Acetate; C005444:N-phenyl-1-naphthylamine; D015057:1-Naphthylamine; C024989:coenzyme Q10