title: Reactive intermediates in cytochrome p450 catalysis.
abstract: Recently, we reported the spectroscopic and kinetic characterizations of cytochrome P450 compound I in CYP119A1, effectively closing the catalytic cycle of cytochrome P450-mediated hydroxylations. In this minireview, we focus on the developments that made this breakthrough possible. We examine the importance of enzyme purification in the quest for reactive intermediates and report the preparation of compound I in a second P450 (P450ST). In an effort to bring clarity to the field, we also examine the validity of controversial reports claiming the production of P450 compound I through the use of peroxynitrite and laser flash photolysis.
authors: Department of Chemistry, Pennsylvania State University, University Park, Pennsylvania 16802, USA.
authors: Courtney M
authors: CM
authors: Krest
authors: Elizabeth L
authors: EL
authors: Onderko
authors: Timothy H
authors: TH
authors: Yosca
authors: Julio C
authors: JC
authors: Calixto
authors: Richard F
authors: RF
authors: Karp
authors: Jovan
authors: J
authors: Livada
authors: Jonathan
authors: J
authors: Rittle
authors: Michael T
authors: MT
authors: Green
mesh_terms: D019843:Archaeal Proteins / Q000737:chemistry* / Q000302:isolation & purification; D055162:Biocatalysis; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D006900:Hydroxylation; D007501:Iron / Q000737:chemistry / Q000302:isolation & purification; D010084:Oxidation-Reduction; D013816:Thermodynamics
chemical_list: D019843:Archaeal Proteins; C066317:ferryl iron; D003577:Cytochrome P-450 Enzyme System; D007501:Iron
keywords: Cytochrome P450; Enzyme Catalysis; Enzyme Purification; Heme; P450 Compound I; Spectroscopy
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