title: Neutralization of interleukin-1 beta attenuates silica-induced lung inflammation and fibrosis in C57BL/6 mice.
abstract: The inflammation and fibrosis induced by silica dust are considered to be substantial responses in silicosis progression. Interleukin-1 beta (IL-1β) plays an important role in silica-induced lung inflammation, but the mechanisms that underlie the influence of IL-1β on the progression of silicosis remain unclear. In this study, the role of IL-1β in silica-induced inflammation and fibrosis was evaluated by administering a suspension of 2.5-mg silica dust, either with or without 40 μg anti-mouse IL-1β monoclonal antibody (mAb), to the lungs of male C57BL/6 mice. Silica + anti-IL-1β mAb-treated mice showed the depletion of IL-1β as well as the attenuation of inflammation, as evaluated in the bronchoalveolar lavage fluid (BALF) and histological sections from 1 to 84 days after silica exposure. Further study of the BALF indicated that inhibition of IL-1β could reduce the contents of tumor necrosis factor-alpha and monocyte chemoattractant protein-1. The real-time PCR and pathology results showed that the neutralization of IL-1β attenuated silica-induced fibrosis by inhibiting the gene expression of transforming growth factor-beta 1, collagen I and fibronectin. The examination of Th1-cytokine and Th2-cytokine suggested that depletion of IL-1β decelerated the Th1/Th2 balance toward a Th2-dominant response. In conclusion, the present study suggests that the neutralization of IL-1β attenuates silica-induced inflammation and fibrosis by inhibiting other inflammatory and fibrogenic mediators and modulating the Th1/Th2 balance.
authors: Department of Occupational and Environmental Health and Ministry of Education Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei, China.
authors: Jiali
authors: J
authors: Guo
authors: Department of Occupational and Environmental Health and Ministry of Education Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei, China.
authors: Nali
authors: N
authors: Gu
authors: Division of Pneumoconiosis, School of Public Health, China Medical University, Shenyang, 110001, Liaoning, China.
authors: Jie
authors: J
authors: Chen
authors: Institute of Health Surveillance, Analysis and Protection, Hubei Center for Disease Control and Prevention, Wuhan, 430070, Hubei, China.
authors: Tingming
authors: T
authors: Shi
authors: Department of Occupational and Environmental Health and Ministry of Education Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei, China.
authors: Yun
authors: Y
authors: Zhou
authors: Department of Occupational and Environmental Health and Ministry of Education Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei, China.
authors: Yi
authors: Y
authors: Rong
authors: Department of Occupational and Environmental Health and Ministry of Education Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei, China.
authors: Ting
authors: T
authors: Zhou
authors: Institute of Health Surveillance, Analysis and Protection, Hubei Center for Disease Control and Prevention, Wuhan, 430070, Hubei, China.
authors: Wenxiang
authors: W
authors: Yang
authors: Department of Occupational and Environmental Health and Ministry of Education Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei, China.
authors: Xiuqing
authors: X
authors: Cui
authors: Department of Occupational and Environmental Health and Ministry of Education Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei, China. wchen@mails.tjmu.edu.cn.
authors: Weihong
authors: W
authors: Chen
mesh_terms: D000818:Animals; D019138:Antibodies, Blocking / Q000494:pharmacology; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002452:Cell Count; D024042:Collagen Type I / Q000096:biosynthesis; D005353:Fibronectins / Q000096:biosynthesis; D066298:In Vitro Techniques; D053583:Interleukin-1beta / Q000037:antagonists & inhibitors*; D008168:Lung / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011650:Pulmonary Alveoli / Q000473:pathology; D011658:Pulmonary Fibrosis / Q000188:drug therapy* / Q000473:pathology; D012822:Silicon Dioxide / Q000633:toxicity*; D012829:Silicosis / Q000188:drug therapy* / Q000473:pathology; D058408:Th1-Th2 Balance / Q000187:drug effects; D016212:Transforming Growth Factor beta / Q000096:biosynthesis
chemical_list: D019138:Antibodies, Blocking; D000911:Antibodies, Monoclonal; D024042:Collagen Type I; D005353:Fibronectins; D053583:Interleukin-1beta; D016212:Transforming Growth Factor beta; D012822:Silicon Dioxide