title: A simple technique based on a single optical trap for the determination of bacterial swimming pattern.
abstract: Bacterial motility is associated to a wide range of biological processes and it plays a key role in the virulence of many pathogens. Here we describe a method to distinguish the dynamic properties of bacteria by analyzing the statistical functions derived from the trajectories of a bacterium trapped by a single optical beam. The approach is based on the model of the rotation of a solid optically trapped sphere. The technique is easily implemented in a biological laboratory, since with only a small number of optical and electronic components a simple biological microscope can be converted into the required analyzer. To illustrate the functionality of this method, we probed several Salmonella enterica serovar Typhimurium mutants that differed from the wild-type with respect to their swimming patterns. In a further application, the motility dynamics of the S. Typhimurium cheV mutant were characterized.
authors: ICFO-Institut de Ciències Fotòniques, Castelldefels, Spain.
authors: Ignacio A
authors: IA
authors: Martínez
authors: Susana
authors: S
authors: Campoy
authors: Meritxell
authors: M
authors: Tort
authors: Montserrat
authors: M
authors: Llagostera
authors: Dmitri
authors: D
authors: Petrov
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D005407:Flagella / Q000378:metabolism; D009068:Movement; D009154:Mutation; D052898:Optical Tweezers; D012486:Salmonella typhimurium / Q000166:cytology / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins
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