title: Expressions of tumor necrosis factor alpha and microRNA-155 in immature rat model of status epilepticus and children with mesial temporal lobe epilepsy.
abstract: Recently, the role of inflammation has attracted great attention in the pathogenesis of mesial temporal lobe epilepsy (MTLE), and microRNAs start to emerge as promising new players in MTLE pathogenesis. In this study, we investigated the dynamic expression patterns of tumor necrosis factor alpha (TNF-α) and microRNA-155 (miR-155) in the hippocampi of an immature rat model of status epilepticus (SE) and children with MTLE. The expressions of TNF-α and miR-155 were significantly upregulated in the seizure-related acute and chronic stages of MTLE in the immature rat model and also in children with MTLE. Modulation of TNF-α expression, either by stimulation using myeloid-related protein (MRP8) or lipopolysaccharide or inhibition using lenalidomide on astrocytes, leads to similar dynamic changes in miR-155 expression. Our study is the first to focus on the dynamic expression pattern of miR-155 in the immature rat of SE lithium-pilocarpine model and children with MTLE and to detect their relationship at the astrocyte level. TNF-α and miR-155, having similar expression patterns in the three stages of MTLE development, and their relationship at the astrocyte level may suggest a direct interactive relationship during MTLE development. Therefore, modulation of the TNF-α/miR-155 axis may be a novel therapeutic target for the treatment of MTLE.
authors: Department of Pediatrics, Xiangya Hospital of Central South University, No. 87 Xiangya Road, Changsha, Hunan, 410008, China.
authors: Muhammad Usman
authors: MU
authors: Ashhab
authors: Ahmed
authors: A
authors: Omran
authors: Huimin
authors: H
authors: Kong
authors: Na
authors: N
authors: Gan
authors: Fang
authors: F
authors: He
authors: Jing
authors: J
authors: Peng
authors: Fei
authors: F
authors: Yin
mesh_terms: D000293:Adolescent; D000818:Animals; D001253:Astrocytes / Q000187:drug effects / Q000378:metabolism; D016022:Case-Control Studies; D002648:Child; D004833:Epilepsy, Temporal Lobe / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006624:Hippocampus / Q000254:growth & development / Q000378:metabolism; D006801:Humans; D000077269:Lenalidomide; D008070:Lipopolysaccharides / Q000494:pharmacology; D018021:Lithium Chloride; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D010862:Pilocarpine; D051381:Rats; D017207:Rats, Sprague-Dawley; D013226:Status Epilepticus / Q000139:chemically induced / Q000378:metabolism*; D013792:Thalidomide / Q000031:analogs & derivatives / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*
chemical_list: D008070:Lipopolysaccharides; C524942:MIRN155 microRNA, human; C580597:MIRN155 microRNA, rat; D035683:MicroRNAs; D014409:Tumor Necrosis Factor-alpha; D010862:Pilocarpine; D013792:Thalidomide; D000077269:Lenalidomide; D018021:Lithium Chloride
references: Neurosci Res. 2012 Feb;72(2):172-80
references: 22138615
references: J Intern Med. 2011 Oct;270(4):319-26
references: 21793950
references: Neurotherapeutics. 2010 Oct;7(4):424-38
references: 20880506
references: J Neurochem. 2005 Feb;92(3):525-35
references: 15659223
references: Neuroscience. 2009 Jun 16;161(1):293-300
references: 19285115
references: Epilepsia. 1999 Apr;40(4):445-52
references: 10219270
references: Glia. 2011 Dec;59(12):1911-22
references: 22170100
references: Immunity. 2010 Oct 29;33(4):607-19
references: 20888269
references: J Immunol. 2007 Oct 15;179(8):5082-9
references: 17911593
references: Epilepsy Res. 2008 Dec;82(2-3):171-6
references: 18783922
references: Epilepsia. 2002;43 Suppl 5:30-5
references: 12121291
references: J Neurosci. 2010 Jun 2;30(22):7484-94
references: 20519523
references: Int J Neurosci. 2011 Oct;121(10):575-88
references: 21812737
references: Neurosci Lett. 2011 Jun 27;497(3):223-30
references: 21362451
references: Neurobiol Dis. 2008 Jan;29(1):142-60
references: 17931873
references: Neuromolecular Med. 2012 Dec;14(4):244-61
references: 22810393
references: Proc Natl Acad Sci U S A. 2009 Feb 24;106(8):2735-40
references: 19193853
references: J Immunol. 1997 Jan 1;158(1):438-45
references: 8977220
references: Nat Rev Mol Cell Biol. 2005 May;6(5):376-85
references: 15852042
references: Epilepsia. 2012 Jul;53(7):1215-24
references: 22708826
references: Ann Neurol. 2005 Jun;57(6):804-12
references: 15852477
references: J Comp Neurol. 1997 Feb 24;378(4):482-92
references: 9034905
references: J Neurosci. 2008 Jul 2;28(27):6904-13
references: 18596165
references: Neurosci Lett. 2012 Nov 7;529(2):133-8
references: 22981977
references: J Cereb Blood Flow Metab. 2010 Jan;30(1):92-101
references: 19724284
references: Epilepsia. 2005;46 Suppl 5:113-7
references: 15987264
references: Nat Med. 2007 Sep;13(9):1042-9
references: 17767165
references: Neurosci Res. 2002 Feb;42(2):147-53
references: 11849734
references: J Biol Chem. 2011 Jan 14;286(2):1436-44
references: 21062749
references: Cell Mol Life Sci. 2012 Sep;69(18):3127-45
references: 22535415
references: Neurology. 2009 May 19;72(20):1718-9
references: 19321843
references: Cardiol Young. 2013 Oct;23(5):642-55
references: 23443043
references: Brain. 2009 Dec;132(Pt 12):3342-52
references: 19952055
references: Brain Res. 2011 Nov 18;1424:53-9
references: 22019057
references: EMBO Mol Med. 2009 Aug;1(5):288-95
references: 19890474
references: Electroencephalogr Clin Neurophysiol. 1972 Mar;32(3):281-94
references: 4110397
references: Epilepsia. 2005 Nov;46(11):1724-43
references: 16302852
references: J Biol Chem. 2011 Apr 1;286(13):11604-15
references: 21282106
references: Acta Neuropathol. 2007 Mar;113(3):235-44
references: 17221203
references: J Mol Neurosci. 2013 Jun;50(2):291-7
references: 23315173
references: Brain Res Mol Brain Res. 2000 Feb 22;75(2):248-58
references: 10686345
references: Eur J Neurosci. 2010 Mar;31(6):1100-7
references: 20214679