title: [Expressions of p-c-jun and cyclinD1 between vascular smooth muscle cells and endothelial cells exposured to cigarette smoke extract].
abstract: OBJECTIVE
  To investigate the cell viabilities of vascular smooth muscle cells and vascular endothelial cells stimulated by cigarette smoke extract(CSE) .
  
  
  METHODS
  The CSE was prepared by smoke-bubbled phosphate buffered saline(PBS) generation.After culturing cells with different concentrations of CSE, we used the cell counting kit-8 to determine the cell viability.The expression levels of c-jun and cyclinD1 were analyzed through Western blot.The c-jun plasmid was transfected to detect the change of cyclinD1 expression.
  
  
  RESULTS
  The smooth muscle cell viability increased when the CSE concentration ranged 0.625%-10%, whereas the endothelial cells viability decreased when exposed to the CSE concentration. After exposure to CSE for 48 hours, there was no difference in c-jun expression between toxin group and PBS group;however, the expression of p-c-jun in the smooth muscle cells significantly increased in the toxin groups than in the PBS group(P<0.05) and the expression of p-c-jun in the vascular endothelial cells significantly decreased(P<0.05) . The level of cyclinD1 significantly increased after exposed to CSE, and its expression level also increased in respond to the c-jun overexpression.
  
  
  CONCLUSIONS
  CSE can enhance the proliferation of vascular smooth muscle cells and decrease in the activity of endothelial cells proliferation, which may be explained by the phosphorylation of c-jun and the expression of cyclinD1.
authors: Department of Vascular Surgery, PUMC Hospital, CAMS and PUMC, Beijing, China.
authors: Tian-jia
authors: TJ
authors: Li
authors: Chang-wei
authors: CW
authors: Liu
authors: Ze-bin
authors: ZB
authors: Huang
authors: Leng
authors: L
authors: Ni
authors: Zhi-chao
authors: ZC
authors: Lai
authors: Li-fei
authors: LF
authors: Wu
authors: Bao
authors: B
authors: Liu
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival; D002478:Cells, Cultured; D019938:Cyclin D1 / Q000378:metabolism*; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism*; D014026:Nicotiana / Q000009:adverse effects*
chemical_list: C530104:CCND1 protein, human; D016755:Proto-Oncogene Proteins c-jun; D019938:Cyclin D1