title: Study of a novel three-dimensional scaffold to repair bone defect in rabbit.
abstract: Both decalcified bone matrix (DBM) and fibrin gel possess good biocompatibility, so they are used as scaffolds to culture bone marrow mesenchymal stem cells (BMSCs). The feasibility and efficacy of using compound material being made of decalcified bone matrix and fibrin gel as a three-dimensional scaffold for bone growth were investigated. BMSCs were isolated from the femur of rabbit, then seeded in prepared scaffolds after incubation for 28 days in vitro. In vivo: 30 New Zealand White Rabbits received bone defect in left radius and divided three treatment groups randomly: (1) BMSCs/decalcified bone matrix/fibrin glue as experimental group; (2) decalcified bone matrix/fibrin glue without cells as control group; (3) nothing was implanted into the bone defects as blank group. The observation period of specimens was 12 weeks, and were analyzed bone formation in terms of serum proteomics (2D-PAGE and MALDI-TOF-TOF-MS), hematoxylin-eosin (HE) staining, ALP staining, and Osteopontin immunofluorescence detection. The experimental group present in three peculiar kinds of proteins, whose Geninfo identifier (GI) number were 136466, 126722803, and 126723746, respectively, correspond to TTR protein, ALB protein, RBP4 protein, and the histological inspections were superior to the other group. The content of osteopontin in experimental group was significantly higher than control group (p <  0.05). The overall results indicated that a combined material being made of BMSCs/decalcified bone matrix/fibrin glue can result in successful bone formation and decalcified bone matrix/fibrin glue admixtures can be used as a scaffold for bone tissue engineering.
authors: Department of Orthopedic Surgery, Orthopedics Implantation Key Lab of Guangdong Province, 1st Affiliated Hospital of Guangzhou Medical University, No.151 Yanjiang Road, Guangzhou, 510120, People's Republic of China.
authors: Yushu
authors: Y
authors: Chen
authors: Bo
authors: B
authors: Bai
authors: Shujiang
authors: S
authors: Zhang
authors: Jing
authors: J
authors: Ye
authors: Haohan
authors: H
authors: Zhai
authors: Yi
authors: Y
authors: Chen
authors: Linlin
authors: L
authors: Zhang
authors: Yanjun
authors: Y
authors: Zeng
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001842:Bone and Bones / Q000201:enzymology / Q000473:pathology*; D015180:Electrophoresis, Gel, Two-Dimensional; D005455:Fluorescent Antibody Technique; D018960:Hyaluronan Receptors / Q000378:metabolism; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D053495:Osteopontin / Q000378:metabolism; D040901:Proteomics; D011817:Rabbits; D044967:Serum / Q000378:metabolism; D054457:Tissue Scaffolds / Q000737:chemistry*; D014945:Wound Healing
chemical_list: D018960:Hyaluronan Receptors; D053495:Osteopontin; D000469:Alkaline Phosphatase
keywords: BMSCs; DBM; bone defect; fibrin glue; serum proteomics