title: Reversible folding of human peripheral myelin protein 22, a tetraspan membrane protein.
abstract: Misfolding of the α-helical membrane protein peripheral myelin protein 22 (PMP22) has been implicated in the pathogenesis of the common neurodegenerative disease known as Charcot-Marie-Tooth disease (CMTD) and also several other related peripheral neuropathies. Emerging evidence suggests that the propensity of PMP22 to misfold in the cell may be due to an intrinsic lack of conformational stability. Therefore, quantitative studies of the conformational equilibrium of PMP22 are needed to gain insight into the molecular basis of CMTD. In this work, we have investigated the folding and unfolding of wild type (WT) human PMP22 in mixed micelles. Both kinetic and thermodynamic measurements demonstrate that the denaturation of PMP22 by n-lauroyl sarcosine (LS) in dodecylphosphocholine (DPC) micelles is reversible. Assessment of the conformational equilibrium indicates that a significant fraction of unfolded PMP22 persists even in the absence of the denaturing detergent. However, we find the stability of PMP22 is increased by glycerol, which facilitates quantitation of thermodynamic parameters. To our knowledge, this work represents the first report of reversible unfolding of a eukaryotic multispan membrane protein. The results indicate that WT PMP22 possesses minimal conformational stability in micelles, which parallels its poor folding efficiency in the endoplasmic reticulum. Folding equilibrium measurements for PMP22 in micelles may provide an approach to assess the effects of cellular metabolites or potential therapeutic agents on its stability. Furthermore, these results pave the way for future investigation of the effects of pathogenic mutations on the conformational equilibrium of PMP22.
authors: Department of Biochemistry and ‡Center for Structural Biology, Vanderbilt University School of Medicine , Nashville, Tennessee 37232, United States.
authors: Jonathan P
authors: JP
authors: Schlebach
authors: Dungeng
authors: D
authors: Peng
authors: Brett M
authors: BM
authors: Kroncke
authors: Kathleen F
authors: KF
authors: Mittendorf
authors: Malathi
authors: M
authors: Narayan
authors: Bruce D
authors: BD
authors: Carter
authors: Charles R
authors: CR
authors: Sanders
mesh_terms: D002942:Circular Dichroism; D003432:Cross-Linking Reagents; D005990:Glycerol; D006801:Humans; D007700:Kinetics; D008823:Micelles; D008958:Models, Molecular; D009185:Myelin Proteins / Q000737:chemistry* / Q000378:metabolism; D011487:Protein Conformation; D017510:Protein Folding; D055550:Protein Stability; D020836:Protein Structure, Quaternary; D013816:Thermodynamics; D056811:Unfolded Protein Response
chemical_list: D003432:Cross-Linking Reagents; D008823:Micelles; D009185:Myelin Proteins; C073687:PMP22 protein, human; D005990:Glycerol
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