title: Neoplastic-like transformation effect of single-walled and multi-walled carbon nanotubes compared to asbestos on human lung small airway epithelial cells.
abstract: Accumulating evidence indicates that carbon nanotubes (CNTs) are biopersistent and can cause lung damage. With similar fibrous morphology and mode of exposure to asbestos, a known human carcinogen, growing concern has arisen for elevated risk of CNT-induced lung carcinogenesis; however, relatively little is known about the long-term carcinogenic effect of CNT. Neoplastic transformation is a key early event leading to carcinogenesis. We studied the ability of single- and multi-walled CNTs to induce neoplastic transformation of human lung epithelial cells compared to asbestos. Long-term (6-month) exposure of the cells to occupationally relevant concentrations of CNT in culture caused a neoplastic-like transformation phenotype as demonstrated by increased cell proliferation, anchorage-independent growth, invasion and angiogenesis. Whole-genome expression signature and protein expression analyses showed that single- and multi-walled CNTs shared similar signaling signatures which were distinct from asbestos. These results provide novel toxicogenomic information and suggest distinct particle-associated mechanisms of neoplasia promotion induced by CNTs and asbestos.
authors: HELD/PPRB, National Institute for Occupational Safety and Health , Morgantown, WV 26505 , USA.
authors: Liying
authors: L
authors: Wang
authors: Todd A
authors: TA
authors: Stueckle
authors: Anurag
authors: A
authors: Mishra
authors: Raymond
authors: R
authors: Derk
authors: Terence
authors: T
authors: Meighan
authors: Vincent
authors: V
authors: Castranova
authors: Yon
authors: Y
authors: Rojanasakul
mesh_terms: D001194:Asbestos / Q000633:toxicity*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000187:drug effects*; D004847:Epithelial Cells / Q000166:cytology; D020869:Gene Expression Profiling; D006801:Humans; D037742:Nanotubes, Carbon / Q000633:toxicity*; D011506:Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D020545:Respiratory Mucosa / Q000166:cytology*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D037742:Nanotubes, Carbon; D011506:Proteins; D001194:Asbestos
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