title: Regulation offimgenes in uropathogenicEscherichia coli.
abstract: UropathogenicEscherichia coli(UPEC) is the leading cause of urinary tract infections in women, causing significant morbidity and mortality in this population. Adherence to host epithelial cells is a pivotal step in the pathogenesis of UPEC. One of the most important virulence factors involved in mediating this attachment is the type 1 pilus (type 1 fimbria) encoded by a set offimgenes arranged in an operon. The expression of type 1 pili is controlled by a phenomenon known as phase variation, which reversibly switches between the expression of type 1 pili (Phase-ON) and loss of expression (Phase-OFF). Phase-ON cells have the promoter for thefimAstructural gene on an invertible DNA element calledfimS, which lines up to allow transcription, whereas transcription of the structural gene is silenced in Phase-OFF cells. The orientation of thefimSinvertible element is controlled by two site-specific recombinases, FimB and FimE. Environmental conditions cause transcriptional and post-transcriptional changes in UPEC cells that affect the level of regulatory proteins, which in turn play vital roles in modulating this phase switching ability. The role offimgene regulation in UPEC pathogenesis will be discussed.
authors: Department of Microbiology, University of Wisconsin-La Crosse, 1725 State Street, La Crosse, WI 54601, United States.
authors: William R
authors: WR
authors: Schwan
keywords: Gene regulation; Type 1 fimbriae; Type 1 pili; Urinary tract; Uropathogenic Escherichia coli
references: J Bacteriol. 2005 Dec;187(24):8256-66
references: 16321930
references: J Bacteriol. 1994 Feb;176(3):610-9
references: 8300516
references: Microbiology (Reading). 2007 Dec;153(Pt 12):4138-4149
references: 18048927
references: J Bacteriol. 1987 Dec;169(12):5530-6
references: 2890622
references: J Bacteriol. 1991 Sep;173(17):5308-14
references: 1679430
references: J Bacteriol. 2005 Sep;187(18):6273-80
references: 16159759
references: J Exp Med. 1977 Nov 1;146(5):1169-81
references: 336832
references: J Bacteriol. 1982 Sep;151(3):1560-7
references: 6125501
references: J Bacteriol. 1999 Feb;181(3):941-8
references: 9922259
references: J Bacteriol. 2006 Aug;188(15):5356-63
references: 16855224
references: J Mol Biol. 1985 May 25;183(2):117-28
references: 3159903
references: J Urol. 2005 Mar;173(3):933-7
references: 15711342
references: J Bacteriol. 1991 Sep;173(17):5298-307
references: 1679429
references: Mol Gen Genet. 1998 Aug;259(3):336-44
references: 9749677
references: Microb Pathog. 1988 Mar;4(3):231-8
references: 2904111
references: Microb Pathog. 2009 May;46(5):243-52
references: 19486641
references: Mol Microbiol. 2003 Aug;49(4):1109-18
references: 12890032
references: Proc Natl Acad Sci U S A. 1987 Sep;84(18):6506-10
references: 2888114
references: Annu Rev Microbiol. 2005;59:379-405
references: 16153174
references: J Clin Microbiol. 1987 Dec;25(12):2253-7
references: 2892855
references: Microbiology (Reading). 2005 Oct;151(Pt 10):3287-3298
references: 16207912
references: Mol Gen Genet. 1985;199(3):410-4
references: 2863734
references: J Bacteriol. 1985 Oct;164(1):321-30
references: 3930469
references: Mol Gen Genet. 1990 Jan;220(2):334-8
references: 1970114
references: Mol Microbiol. 1989 Jul;3(7):925-31
references: 2571915
references: J Bacteriol. 1993 Jan;175(1):27-36
references: 8093239
references: Mol Microbiol. 1998 Apr;28(2):371-81
references: 9622361
references: Infect Immun. 1981 Dec;34(3):708-11
references: 6120897
references: J Bacteriol. 1992 Apr;174(7):2367-75
references: 1348054
references: Mol Microbiol. 1996 Aug;21(4):725-38
references: 8878036
references: J Bacteriol. 1984 Oct;160(1):61-6
references: 6148338
references: Annu Rev Biochem. 1984;53:293-321
references: 6236744
references: Infect Immun. 1988 May;56(5):1023-9
references: 2895738
references: J Bacteriol. 2000 May;182(10):2953-9
references: 10781567
references: Mol Microbiol. 1998 Feb;27(4):751-61
references: 9515701
references: Science. 1981 Oct 16;214(4518):337-9
references: 6116279
references: J Bacteriol. 1984 Aug;159(2):736-44
references: 6146599
references: Proc Natl Acad Sci U S A. 1990 Jan;87(1):360-3
references: 2404280
references: J Bacteriol. 2004 Jan;186(1):226-34
references: 14679242
references: Mol Gen Genet. 1994 Mar;242(5):623-30
references: 8121417
references: J Bacteriol. 1999 Jan;181(2):563-71
references: 9882671
references: J Bacteriol. 1993 Oct;175(19):6186-93
references: 8104927
references: Infect Immun. 1988 Oct;56(10):2615-22
references: 2901404
references: Mol Microbiol. 2000 Apr;36(2):457-66
references: 10792731
references: Microbiology (Reading). 1999 Oct;145 ( Pt 10):2683-90
references: 10537190
references: Cell. 1990 Nov 2;63(3):631-42
references: 2171779
references: J Bacteriol. 2007 Oct;189(19):7159-63
references: 17644608
references: FEMS Microbiol Lett. 1994 Feb 1;116(1):95-100
references: 7510658
references: Mol Microbiol. 2001 Oct;42(2):319-30
references: 11703657
references: EMBO J. 1986 Jun;5(6):1389-93
references: 2874022
references: Nat Rev Microbiol. 2004 May;2(5):391-400
references: 15100692
references: Infect Immun. 1998 Jul;66(7):3303-10
references: 9632599
references: Mol Microbiol. 2003 Apr;48(2):287-94
references: 12675791
references: Mol Microbiol. 1997 Sep;25(5):871-82
references: 9364913
references: Eur J Biochem. 1984 Sep 3;143(2):395-9
references: 6147250
references: Nature. 1996 May 2;381(6577):77-80
references: 8609993
references: Mol Gen Genet. 1997 Mar 18;254(1):13-20
references: 9108285
references: Infect Immun. 1987 Feb;55(2):373-80
references: 2879794
references: J Bacteriol. 1992 Sep;174(18):5923-35
references: 1355769
references: J Bacteriol. 1986 Mar;165(3):1033-6
references: 2419305
references: Cell. 1980 Jul;20(3):711-9
references: 6251971
references: Mol Microbiol. 2002 Aug;45(4):1107-17
references: 12180928
references: FEMS Microbiol Lett. 1992 Dec 1;78(2-3):125-30
references: 1362708
references: J Mol Biol. 1991 Feb 20;217(4):661-79
references: 1848637
references: J Bacteriol. 1987 Mar;169(3):934-8
references: 2880835
references: J Bacteriol. 1985 May;162(2):668-75
references: 2859269
references: Infect Immun. 1986 Sep;53(3):693-6
references: 2875030
references: Infect Immun. 1985 Nov;50(2):370-7
references: 2865209
references: PLoS Pathog. 2009 Feb;5(2):e1000303
references: 19229313
references: J Bacteriol. 1983 Dec;156(3):1052-8
references: 6139362
references: Mol Microbiol. 1996 Sep;21(6):1161-73
references: 8898385
references: Mol Gen Genet. 1995 Jun 10;247(5):521-8
references: 7603430
references: J Bacteriol. 2007 Aug;189(15):5523-33
references: 17513470
references: Mol Microbiol. 1999 Feb;31(4):1171-81
references: 10096084
references: J Bacteriol. 1978 Apr;134(1):356-8
references: 206537
references: J Bacteriol. 1985 Apr;162(1):454-7
references: 2858471
references: Mol Microbiol. 2006 Jun;60(6):1520-33
references: 16796685
references: Proc Natl Acad Sci U S A. 2005 May 31;102(22):7823-8
references: 15899978
references: J Bacteriol. 1987 Aug;169(8):3840-3
references: 2886490
references: Proc Natl Acad Sci U S A. 2004 Nov 16;101(46):16322-7
references: 15534208
references: Cell. 1988 Feb 26;52(4):569-84
references: 2830029
references: Infect Immun. 2002 Jul;70(7):3344-54
references: 12065472
references: Mol Microbiol. 1999 Dec;34(5):965-79
references: 10594822
references: Genes Dev. 1989 Jan;3(1):123-30
references: 2468557
references: Proc Natl Acad Sci U S A. 1995 Mar 14;92(6):2081-5
references: 7892228
references: Infect Immun. 2005 Nov;73(11):7588-96
references: 16239562
references: FEMS Microbiol Lett. 2000 Jan 15;182(2):319-25
references: 10620686
references: EMBO J. 1984 Dec 1;3(12):2885-8
references: 6151898
references: Mol Microbiol. 2009 Dec;74(5):1071-82
references: 19889099
references: Infect Immun. 1989 Jun;57(6):1656-62
references: 2566580
references: J Bacteriol. 1981 Jan;145(1):620-3
references: 6109708
references: Proc Natl Acad Sci U S A. 1996 Sep 3;93(18):9827-32
references: 8790416
references: Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8397-401
references: 8104335
references: J Bacteriol. 1991 Jul;173(13):4116-23
references: 1648076
references: EMBO J. 2000 Apr 3;19(7):1450-7
references: 10747013
references: FEMS Microbiol Lett. 1994 Oct 1;122(3):281-7
references: 7988869
references: Microbiology (Reading). 2006 Apr;152(Pt 4):1143-1153
references: 16549677
references: Mol Gen Genet. 1987 Jul;208(3):439-45
references: 2890081
references: J Infect Dis. 1987 Oct;156(4):567-74
references: 2887621
references: Infect Immun. 1983 Apr;40(1):273-83
references: 6339403
references: Infect Immun. 2001 May;69(5):2838-46
references: 11292696
references: J Bacteriol. 1999 Jan;181(1):47-54
references: 9864311
references: Infect Immun. 2008 Sep;76(9):4129-36
references: 18591231
references: Infect Immun. 2002 Mar;70(3):1391-402
references: 11854225
references: Infect Immun. 1990 Jun;58(6):1995-8
references: 1971261
references: Mol Microbiol. 2007 Feb;63(4):1223-36
references: 17238917
references: FEMS Microbiol Lett. 1998 May 1;162(1):17-23
references: 9595659
references: Infect Immun. 2006 Feb;74(2):1072-83
references: 16428754
references: Microbiol Mol Biol Rev. 2002 Sep;66(3):373-95, table of contents
references: 12208995
references: Mol Microbiol. 1995 Jul;17(1):109-21
references: 7476197
references: Mol Microbiol. 2001 Oct;42(2):483-94
references: 11703669
references: Nature. 1990 Apr 12;344(6267):682-5
references: 1691451
references: J Bacteriol. 1987 Feb;169(2):640-5
references: 2879830
references: J Bacteriol. 1994 Sep;176(18):5665-72
references: 7916011
references: Nature. 1988 Mar 17;332(6161):265-8
references: 2894612
references: J Bacteriol. 1997 Nov;179(21):6618-25
references: 9352908
references: Mol Microbiol. 1997 Feb;23(4):705-17
references: 9157242
references: PLoS Comput Biol. 2010 Mar 26;6(3):e1000723
references: 20361050
references: Proc Natl Acad Sci U S A. 1985 Sep;82(17):5724-7
references: 2863818
references: Mol Microbiol. 2008 Jan;67(1):171-87
references: 18034794
references: J Bacteriol. 1986 Oct;168(1):179-85
references: 3019997
references: J Biol Chem. 2011 Sep 16;286(37):32026-35
references: 21768111
references: Mol Microbiol. 2008 Mar;67(6):1223-41
references: 18284577
references: Infect Immun. 1979 Apr;24(1):218-23
references: 378843
references: J Bacteriol. 2003 Aug;185(16):5007-11
references: 12897022
references: Infect Dis Clin North Am. 2003 Jun;17(2):227-41
references: 12848468
references: J Bacteriol. 1993 Jul;175(14):4335-44
references: 8101185
references: Mol Microbiol. 1990 Jul;4(7):1077-82
references: 1700256
references: Infect Immun. 2006 Feb;74(2):1387-93
references: 16428790
references: Infect Immun. 2006 Jul;74(7):4039-47
references: 16790777
references: EMBO J. 1985 Dec 30;4(13B):3887-93
references: 2868893