title: Overexpression of a GmGBP1 ortholog of soybean enhances the responses to flowering, stem elongation and heat tolerance in transgenic tobaccos.
abstract: Soybean is a typical short-day crop, and its photoperiodic and gibberellin (GA) responses for the control of flowering are critical to seed yield. The GmGBP1 mRNA abundance in leaves was dramatically increased in short-days (SDs) compared to that in long-days in which it was consistently low at all time points from 0 to 6 days (days after transfer to SDs). GmGBP1 was highly expressed in leaves and exhibited a circadian rhythm in SDs. Ectopic overexpression of GmGBP1 in tobaccos caused photoperiod-insensitive early flowering by increasing NtCO mRNA levels. GmGBP1 mRNA abundance was also increased by GAs. Transgenic GmGBP1 overexpressing (-ox) tobacco plants exhibited increased GA signaling-related phenotypes including flowering and plant height promotion. Furthermore, the hypocotyl elongation, early-flowering and longer internode phenotypes were largely accelerated by GA3 application in the GmGBP1-ox tobacco seedlings. Being consistent, overexpression of GmGBP1 resulted in significantly enhanced GA signaling (evidenced suppressed expression of NtGA20ox) both with and without GA treatments. GmGBP1 was a positive regulator of both photoperiod and GA-mediated flowering responses. In addition, GmGBP1-ox tobaccos were hypersensitive to ABA, salt and osmotic stresses during seed germination. Heat-inducible GmGBP1 also enhanced thermotolerance in transgenic GmGBP1-ox tobaccos during seed germination and growth. GmGBP1 protein was localized in the nucleus. Analyses of a series of 5'-deletions of the GmGBP1 promoter suggested that several cis-acting elements, including P-BOX, TCA-motif and three HSE elements necessary to induce gene expression by GA, salicic acid and heat stress, were specifically localized in the GmGBP1 promoter region.
authors: Key Laboratory of Soybean Biology of Chinese Education Ministry (Key Laboratory of Biology and Genetics & Breeding for Soybean in Northeast China), Northeast Agricultural University, Harbin 150030, China. zlhappy1981@yahoo.com.cn
authors: Lin
authors: L
authors: Zhao
authors: Zhixin
authors: Z
authors: Wang
authors: Qingyao
authors: Q
authors: Lu
authors: Pengpeng
authors: P
authors: Wang
authors: Yongguang
authors: Y
authors: Li
authors: Qingxue
authors: Q
authors: Lv
authors: Xianping
authors: X
authors: Song
authors: Dongmei
authors: D
authors: Li
authors: Yuejiao
authors: Y
authors: Gu
authors: Lixue
authors: L
authors: Liu
authors: Wenbin
authors: W
authors: Li
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics; D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development; D001483:Base Sequence; D002467:Cell Nucleus; D035264:Flowers / Q000235:genetics* / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000528:radiation effects; D005875:Gibberellins / Q000494:pharmacology; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006358:Hot Temperature; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D017440:Photoperiod; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018547:Plant Stems / Q000235:genetics* / Q000254:growth & development / Q000648:ultrastructure; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012965:Sodium Chloride / Q000494:pharmacology; D030262:Soybean Proteins / Q000235:genetics* / Q000378:metabolism; D013025:Glycine max / Q000235:genetics* / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics* / Q000254:growth & development
chemical_list: D005875:Gibberellins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D030262:Soybean Proteins; D049452:Green Fluorescent Proteins; D012965:Sodium Chloride
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