title: Live imaging, identifying, and tracking single cells in complex populations in vivo and ex vivo.
abstract: Advances in optical imaging technologies combined with the use of genetically encoded fluorescent proteins have enabled the visualization of stem cells over extensive periods of time in vivo and ex vivo. The generation of genetically encoded fluorescent protein reporters that are fused with subcellularly localized proteins, such as human histone H2B, has made it possible to direct fluorescent protein reporters to specific subcellular structures and identify single cells in complex populations. This facilitates the visualization of cellular behaviors such as division, movement, and apoptosis at a single-cell resolution and, in principle, allows the prospective and retrospective tracking towards determining the lineage of each cell.
authors: Developmental Biology Program, Sloan-Kettering Institute, New York, NY, USA.
authors: Minjung
authors: M
authors: Kang
authors: Panagiotis
authors: P
authors: Xenopoulos
authors: Silvia
authors: S
authors: Muñoz-Descalzo
authors: Xinghua
authors: X
authors: Lou
authors: Anna-Katerina
authors: AK
authors: Hadjantonakis
mesh_terms: D000818:Animals; D017209:Apoptosis; D018929:Cell Culture Techniques; D002455:Cell Division; D002465:Cell Movement; D049109:Cell Proliferation; D058948:Cell Tracking / Q000379:methods*; D004622:Embryo, Mammalian; D053595:Embryonic Stem Cells; D005453:Fluorescence; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008164:Luminescent Proteins / Q000378:metabolism; D051379:Mice; D061848:Optical Imaging / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000378:metabolism
chemical_list: D008164:Luminescent Proteins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins
references: Mol Plant. 2011 Sep;4(5):922-31
references: 21965456
references: IEEE Trans Image Process. 2005 Sep;14(9):1396-410
references: 16190474
references: IEEE Trans Image Process. 2010 Mar;19(3):770-81
references: 19955038
references: Med Image Anal. 2011 Aug;15(4):650-68
references: 20864383
references: Proc Natl Acad Sci U S A. 2006 Feb 21;103(8):2707-12
references: 16477039
references: IEEE Trans Med Imaging. 2008 Jun;27(6):789-804
references: 18541486
references: Genesis. 2006 Apr;44(4):202-18
references: 16604528
references: Nat Rev Genet. 2003 Aug;4(8):613-25
references: 12897773
references: Dev Biol. 2011 Jul 15;355(2):239-49
references: 21539832
references: Nat Methods. 2010 Aug;7(8):637-42
references: 20601950
references: Cold Spring Harb Protoc. 2011 Apr 01;2011(4):pdb.top104
references: 21460058
references: Nat Rev Microbiol. 2009 May;7(5):383-92
references: 19369953
references: Nature. 2007 Dec 20;450(7173):1230-4
references: 18097409
references: Development. 2008 Mar;135(5):909-18
references: 18263842
references: Science. 2008 Nov 14;322(5904):1065-9
references: 18845710
references: Cytometry A. 2009 Apr;75(4):289-97
references: 18951464
references: Nat Methods. 2011 Apr;8(4 Suppl):S30-5
references: 21451514
references: IEEE Trans Med Imaging. 2010 Jan;29(1):96-105
references: 19643704
references: Trends Biotechnol. 2009 May;27(5):266-76
references: 19339068
references: Development. 2012 Sep;139(17):3071-80
references: 22872081
references: Science. 2010 Aug 20;329(5994):967-71
references: 20724640
references: BMC Biotechnol. 2004 Dec 24;4:33
references: 15619330
references: IEEE Trans Med Imaging. 2010 Mar;29(3):852-67
references: 20199920
references: Semin Cell Dev Biol. 2009 Oct;20(8):894-902
references: 19660567
references: Nat Methods. 2010 Jul;7(7):547-53
references: 20543845
references: Genesis. 2009 May;47(5):330-6
references: 19358158
references: Genome Biol. 2006;7(10):R100
references: 17076895
references: Med Image Anal. 2008 Oct;12(5):546-66
references: 18656418