title: [Detection of phenolphthalein added illegally into anti-obesity and healthcare food by TLC-FTIR].
abstract: OBJECTIVE
  To establish a TLC-FTIR method for detection of western medicine phenolphthalein added illegally into anti-obesity and healthcare food.
  
  
  METHODS
  The sample was extracted with anhydrous alcohol. The stationary phase was the GF254 aluminium alloy silica gel plates (10 cm x 20 cm) while the developing solvent was acetic ether: petroleum ether (60-90): methanol = 10: 6: 1. The sample volume was 2 microl. After primary screening by the UV lamp 254nm and self-made 2% NaOH test paper, the preparative technique of TLC was used to separate the component. Then the component was detected by FTIR and compared with the FTIR spectrogram of standard substance.
  
  
  RESULTS
  Five of the ten samples contained phenolphthalein with the same testing results by using the TLC scanning and HPLC.
  
  
  CONCLUSIONS
  The established method is accurate and reliable and can be used for detection of phenolphthalein illegally added into the products.
authors: School of Pharmacy, Qiqihar Medical University, Qiqihar 161006, China.
authors: Feng
authors: F
authors: Xu
authors: Ying
authors: Y
authors: Yang
authors: Li
authors: L
authors: Li
authors: Leping
authors: L
authors: Wang
mesh_terms: D002855:Chromatography, Thin Layer / Q000379:methods; D019587:Dietary Supplements / Q000009:adverse effects / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D006801:Humans; D009765:Obesity / Q000517:prevention & control*; D020113:Phenolphthalein / Q000032:analysis*; D015203:Reproducibility of Results; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods
chemical_list: D020113:Phenolphthalein