title: Magnetofection of human somatic cells with magnetite and cobalt ferrospinel nanoparticles.
abstract: Superparamagnetic nanoparticles varying by their chemical composition and synthesis method were used to transfer DNA into somatic cells under the influence of constant magnetic field (method of magnetofection). Magnetite particles obtained by mechanochemical synthesis ensured higher expression of the marker gene GFP (evaluated by fluorescence intensity of the cell lysate) then particles of ferric oxide obtained by chemical co-precipitation and cobalt ferrospinel particles obtained by the mechanochemical method.
authors: Institute of Chemical Biology and Fundumental Medicine, Siberian Division of the Russian Academy of Sciences, Russia. marina_sukoyan@mail.ru
authors: M A
authors: MA
authors: Sukoyan
authors: E A
authors: EA
authors: Khrapov
authors: E N
authors: EN
authors: Voronina
authors: U A
authors: UA
authors: Boyarskikh
authors: A I
authors: AI
authors: Gubanov
authors: V I
authors: VI
authors: Itin
authors: A A
authors: AA
authors: Magaeva
authors: E P
authors: EP
authors: Nayden
authors: O G
authors: OG
authors: Terekhova
authors: M L
authors: ML
authors: Filipenko
mesh_terms: D002460:Cell Line; D003035:Cobalt; D004247:DNA / Q000378:metabolism*; D004337:Drug Carriers; D005290:Ferric Compounds / Q000737:chemistry; D052203:Ferrosoferric Oxide / Q000737:chemistry; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000235:genetics*; D057809:HEK293 Cells; D006801:Humans; D060328:Magnetic Phenomena; D058185:Magnetite Nanoparticles
chemical_list: D004337:Drug Carriers; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; C001215:ferrite; D049452:Green Fluorescent Proteins; C000499:ferric oxide; D003035:Cobalt; D004247:DNA; D052203:Ferrosoferric Oxide