title: Estimation of serum malondialdehyde in potentially malignant disorders and post-antioxidant treated patients: A biochemical study.
abstract: BACKGROUND
  Tobacco causes the generation of free radicals and reactive oxygen species (ROS) which are responsible for the high rate of lipid peroxidation. Malondialdehyde (MDA) is the most widely used agent to estimate the extent of lipid peroxidation. Timely diagnosis of the condition followed by supplementation with antioxidants like beta-carotene, pro-vitamin A, vitamin A, vitamin C, vitamin E, lipoic acid, zinc, selenium, and spirulina can prevent potentially malignant disorders.
  
  
  METHODS
  In this study, serum MDA was measured according to the method of Buege, in 15 normal samples and 15 patients who were histopathologically diagnosed with potentially malignant disordered and they were prescribed with antioxidants for a period of 4 week-time following which potentially malignant patients serum MDA was analyzed again to determine the extent of peroxidation reactions.
  
  
  RESULTS
  The mean serum MDA level in Group C1 was 0.7900 ± 0.2336 μM/L were as the mean serum MDA level of Group S1 was 2.478 ± 0.50756 μM/L and the values between them were highly significant. The values between C1 and S2 were found to be statistically significant. The mean serum MDA of S2 was 2.160 ± 0.41252 μM/L and the values were significant when compared to S1.
  
  
  CONCLUSIONS
  Serum MDA estimation in oral pre-cancer would serve in determining the extent of lipid peroxidation. Diagnosis of patients and administration of antioxidants has proven to be effective in declining the ROS and thus reducing the extent of damage on the cells. MDA may serve as a diagnostic tool in the estimation of oral pre-cancer and in evaluation of post-treated cases.
authors: Department of Oral Medicine and Radiology, A B Shetty Memorial Institute of Dental Sciences, Nitte University, Deralakatte, Mangalore, India.
authors: Deepa
authors: D
authors: D'souza
authors: Babu G
authors: BG
authors: Subhas
authors: Shishir Ram
authors: SR
authors: Shetty
authors: Preethi
authors: P
authors: Balan
keywords: Antioxidants; free radicals; lipid peroxidation; malondialdehyde
references: Clin Mol Pathol. 1996 Oct;49(5):M247-55
references: 16696085
references: Life Sci. 1991;48(4):301-9
references: 1990230
references: Indian J Clin Biochem. 2004 Jan;19(1):138-41
references: 23105446
references: Oral Oncol. 1998 Jan;34(1):24-9
references: 9659516
references: Clin Chem. 1995 Dec;41(12 Pt 2):1819-28
references: 7497639
references: J Assoc Physicians India. 2004 Oct;52:794-804
references: 15909857
references: J Cancer Res Ther. 2010 Oct-Dec;6(4):487-91
references: 21358086
references: Indian J Cancer. 2004 Jan-Mar;41(1):25-31
references: 15105576
references: Free Radic Biol Med. 1990;9(6):515-40
references: 2079232
references: J Can Dent Assoc. 2000 May;66(5):252-6
references: 10833868
references: J Am Coll Nutr. 2000 Oct;19(5):563-9
references: 11022869
references: Annu Rev Nutr. 1996;16:33-50
references: 8839918
references: Gynecol Obstet Invest. 1999;48(3):197-9
references: 10545746