title: Real-time dynamic optical imaging of ACC-M tumor cells killed by HSV-tk/ACV system.
abstract: HSV-tk/ACV induced and killed human adenoid cystic carcinoma cell (ACC-M) in vivo and in vitro, which were observed through optical imaging and green fluorescence protein (GFP) tagging technique. ACC-M was transfected with TK-GFP, and the single clone cell ACC-M-TK-GFP was selected by G418. With fluorescent stereomicroscope, whole-body fluorescent imaging system and fluorescent microscope, we could observe ACV treated ACC-M-TK-GFP cells in cell level and nude mice. The therapies of tumor were visualized clearly with optical imaging. This study proves that optical imaging is a very good approach for studying the effect of HSV-tk/ACV on the ACC-M tumor cells and decreasing the amount of vessel about tumors cell. Optical imaging will become a visual groundwork for monitoring tumor growth and evaluating in vivo curative effect of antitumor drugs.
authors: College of Life Science, Yangtze University, Jingzhou 434025, Hubei, China.
authors: Tao
authors: T
authors: Xiong
authors: Yongjin
authors: Y
authors: Li
authors: Zhiyang
authors: Z
authors: Li
authors: Xiangmo
authors: X
authors: Xie
authors: Lisha
authors: L
authors: Lu
mesh_terms: D000212:Acyclovir / Q000008:administration & dosage; D000818:Animals; D000998:Antiviral Agents / Q000008:administration & dosage; D003528:Carcinoma, Adenoid Cystic / Q000235:genetics / Q000473:pathology* / Q000628:therapy*; D045744:Cell Line, Tumor; D003199:Computer Systems; D049452:Green Fluorescent Proteins; D018259:Herpesvirus 1, Human / Q000201:enzymology*; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008856:Microscopy, Fluorescence / Q000379:methods*; D013937:Thymidine Kinase / Q000235:genetics* / Q000627:therapeutic use*; D016896:Treatment Outcome; D051598:Whole Body Imaging / Q000379:methods
chemical_list: D000998:Antiviral Agents; D049452:Green Fluorescent Proteins; D013937:Thymidine Kinase; D000212:Acyclovir