title: Characteristics of Streptococcus mutans genotypes and dental caries in children.
abstract: This longitudinal cohort study evaluated the diversity, commonality, and stability of Streptococcus mutans genotypes associated with dental caries history. Sixty-seven 5- and 6-yr-old children, considered as being at high caries risk, had plaque collected from baseline through 36 months for S. mutans isolation and genotyping using repetitive extragenic palindromic-PCR (4,392 total isolates). Decayed, missing, or filled surfaces (dmfs (primary teeth)/DMFS (secondary teeth)) for each child were recorded at baseline. At baseline, 18 distinct genotypes were found among 911 S. mutans isolates from 67 children (diversity), and 13 genotypes were shared by at least two children (commonality). The number of genotypes per individual was positively associated with the proportion of decayed surfaces (p-ds) at baseline. Twenty-four of the 39 children who were available at follow-up visits maintained a predominant genotype for the follow-up periods (stability) and this was negatively associated with the p-ds. The observed diversity, commonality, and stability of S. mutans genotypes represent a pattern of dental caries epidemiology in this high-caries-risk community, which suggests that fewer decayed surfaces are significantly associated with lower diversity and higher stability of S. mutans genotypes.
authors: Department of Pediatric Dentistry, University of Alabama School of Dentistry, Birmingham, AL 35294, USA.
authors: Kyounga
authors: K
authors: Cheon
authors: Stephen A
authors: SA
authors: Moser
authors: Howard W
authors: HW
authors: Wiener
authors: Jennifer
authors: J
authors: Whiddon
authors: Stephanie S
authors: SS
authors: Momeni
authors: John D
authors: JD
authors: Ruby
authors: Gary R
authors: GR
authors: Cutter
authors: Noel K
authors: NK
authors: Childers
mesh_terms: D001741:Black or African American / Q000235:genetics*; D000407:Alabama / Q000453:epidemiology; D016009:Chi-Square Distribution; D002648:Child; D002675:Child, Preschool; D015169:Colony Count, Microbial; D004245:DMF Index; D016172:DNA Fingerprinting / Q000379:methods*; D004269:DNA, Bacterial / Q000032:analysis; D003731:Dental Caries / Q000453:epidemiology / Q000382:microbiology*; D003773:Dental Plaque / Q000382:microbiology; D005500:Follow-Up Studies; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008137:Longitudinal Studies; D020411:Oligonucleotide Array Sequence Analysis; D015995:Prevalence; D012463:Saliva / Q000382:microbiology; D018709:Statistics, Nonparametric; D013295:Streptococcus mutans / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial
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