title: Myofibroblastic differentiation of stromal cells in giant cell tumor of bone: an immunohistochemical and ultrastructural study.
abstract: The nature of the mononuclear stromal cells (MSCs) in giant cell tumor of bone (GCTB) has not been thoroughly investigated. The purpose of this study was to evaluate the degree and significance of myofibroblastic differentiation in 18 cases of GCTB by immunohistochemistry (IH) and/or electron microscopy (EM). All immunostained cases were found positive for smooth muscle actin (SMA) and/or muscle specific actin (MSA), most in 1-33% of the MSCs. Ultrastructurally, most MSCs were fibroblasts, and a significant number of cells displayed myofibroblastic differentiation. Myofibroblasts are an important component of MSCs in GCTB. The myofibroblastic population may be responsible in part for the production of matrix metalloproteinases (MMPs), which probably play a role in bone destruction, tumor aggression, and recurrence.
authors: Department of Pathology, Mount Sinai Medical Center, New York, NY 10029, USA. Roberto.Garcia@mountsinai.org
authors: Roberto A
authors: RA
authors: Garcia
authors: Corneliu D
authors: CD
authors: Platica
authors: M
authors: M
authors: Alba Greco
authors: German C
authors: GC
authors: Steiner
mesh_terms: D000199:Actins / Q000032:analysis; D000293:Adolescent; D000328:Adult; D014408:Biomarkers, Tumor / Q000032:analysis*; D001706:Biopsy; D001859:Bone Neoplasms / Q000737:chemistry / Q000648:ultrastructure; D002454:Cell Differentiation; D005260:Female; D018212:Giant Cell Tumor of Bone / Q000737:chemistry / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008854:Microscopy, Electron; D008875:Middle Aged; D058628:Myofibroblasts / Q000737:chemistry / Q000648:ultrastructure; D011237:Predictive Value of Tests; D011379:Prognosis; D017154:Stromal Cells / Q000737:chemistry / Q000648:ultrastructure; D055815:Young Adult
chemical_list: D000199:Actins; D014408:Biomarkers, Tumor