title: Characterization of cerebrospinal fluid by comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry.
abstract: Comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC×GC-TOFMS) was applied in the quantification and identification of organic compounds in patient-matched human cerebrospinal fluid (CSF) and serum samples. Concentrations of 21 amino and hydroxyl acids varied from 0.04 to 77ng/μl in CSF and from 0.1 to 84ng/μl in serum. In total, 91 metabolites out of over 1200 detected were identified based on mass spectra and retention indices. The other metabolites were identified at the functional group level. The main metabolites detected in CSF were sugar and amino acid derivatives. The CSF and serum had clearly distinct metabolic profiles, with larger biological variation in the serum than in CSF. The GC×GC-TOFMS allowed detection and identification of several metabolites that have not been previously detected in CSF.
authors: VTT Technical Research Centre of Finland, P.O. Box 1000, FI-02044 VTT, Finland.
authors: Minna
authors: M
authors: Hartonen
authors: Ismo
authors: I
authors: Mattila
authors: Anna-Liisa
authors: AL
authors: Ruskeepää
authors: Matej
authors: M
authors: Orešič
authors: Tuulia
authors: T
authors: Hyötyläinen
mesh_terms: D000368:Aged; D000596:Amino Acids / Q000097:blood / Q000134:cerebrospinal fluid* / Q000737:chemistry; D002241:Carbohydrates / Q000097:blood / Q000134:cerebrospinal fluid* / Q000737:chemistry; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods* / Q000592:standards*; D006801:Humans; D008055:Lipids / Q000097:blood / Q000134:cerebrospinal fluid* / Q000737:chemistry; D008297:Male; D055442:Metabolome; D015203:Reproducibility of Results
chemical_list: D000596:Amino Acids; D002241:Carbohydrates; D008055:Lipids