title: Expression analysis of aquaporins from desert truffle mycorrhizal symbiosis reveals a fine-tuned regulation under drought.
abstract: We have performed the isolation, functional characterization, and expression analysis of aquaporins in roots and leaves of Helianthemum almeriense, in order to evaluate their roles in tolerance to water deficit. Five cDNAs, named HaPIP1;1, HaPIP1;2, HaPIP2;1, HaPIP2;2, and HaTIP1;1, were isolated from H. almeriense. A phylogenetic analysis of deduced proteins confirmed that they belong to the water channel proteins family. The HaPIP1;1, HaPIP2;1, and HaTIP1;1 genes encode functional water channel proteins, as indicated by expression assays in Saccharomyces cerevisiae, showing divergent roles in the transport of water, CO2, and NH3. The expression patterns of the genes isolated from H. almeriense and of a previously described gene from Terfezia claveryi (TcAQP1) were analyzed in mycorrhizal and nonmycorrhizal plants cultivated under well-watered or drought-stress conditions. Some of the studied aquaporins were subjected to fine-tuned expression only under drought-stress conditions. A beneficial effect on plant physiological parameters was observed in mycorrhizal plants with respect to nonmycorrhizal ones. Moreover, stress induced a change in the mycorrhizal type formed, which was more intracellular under drought stress. The combination of a high intracellular colonization, together with the fine-tuned expression of aquaporins could result in a morphophysiological adaptation of this symbiosis to drought conditions.
authors: Departamento Biología Vegetal Botánica, Facultad de Biología, Universidad de Murcia, Murcia, Spain.
authors: Alfonso
authors: A
authors: Navarro-Ródenas
authors: Gloria
authors: G
authors: Bárzana
authors: Emilio
authors: E
authors: Nicolás
authors: Andrea
authors: A
authors: Carra
authors: Andrea
authors: A
authors: Schubert
authors: Asunción
authors: A
authors: Morte
mesh_terms: D000595:Amino Acid Sequence; D020346:Aquaporins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D001203:Ascomycota / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D001692:Biological Transport; D030601:Cistaceae / Q000235:genetics* / Q000254:growth & development / Q000382:microbiology / Q000502:physiology; D055864:Droughts; D005656:Fungal Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D018506:Gene Expression Regulation, Plant; D008969:Molecular Sequence Data; D038821:Mycorrhizae / Q000235:genetics* / Q000254:growth & development / Q000502:physiology; D010788:Photosynthesis; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development / Q000382:microbiology / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000254:growth & development / Q000382:microbiology / Q000502:physiology; D018520:Plant Shoots / Q000235:genetics / Q000254:growth & development / Q000382:microbiology / Q000502:physiology; D018526:Plant Transpiration; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013312:Stress, Physiological; D013559:Symbiosis; D014867:Water / Q000378:metabolism
chemical_list: D020346:Aquaporins; D005656:Fungal Proteins; D010940:Plant Proteins; D014867:Water