title: On-line surface plasmon resonance-high performance liquid chromatography-tandem mass spectrometry for analysis of human serum albumin binders from Radix Astragali.
abstract: Development of sensitive and effective methods that meet the demand of high-throughput, high-fidelity screening of bioactive components from natural products are important to drug discovery. We describe here a novel surface plasmon resonance-high performance liquid chromatography-tandem mass spectrometry (SPR-HPLC-MS/MS) system for rapid, continuous and effective screening and identification of human serum albumin (HSA) binders from Radix Astragali. The HPLC six-port valve was used as interface through which the dissociated HSA binders from SPR was collected and injected automatically to HPLC-MS/MS for analysis. Eleven isofalvonoids and nine astragalosides have been screened and identified as the main HSA binders in ethyl acetate extract of Radix Astragali. Compared with reverse ultrafiltration assay, the developed configuration is more cost effective and reproductive because of the higher reusability and stability of immobilized HSA on SPR chip. Furthermore, the assay minimized the matrix interference in MS because of the elimination of non-binding components before HPLC separation. It is concluded that this novel technology offers new perspectives for screening and identification of active components from complex mixtures.
authors: School of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
authors: Yuping
authors: Y
authors: Zhang
authors: Shuyun
authors: S
authors: Shi
authors: Junfang
authors: J
authors: Guo
authors: Qingping
authors: Q
authors: You
authors: Deshan
authors: D
authors: Feng
mesh_terms: D027883:Astragalus Plant / Q000737:chemistry / Q000378:metabolism; D027885:Astragalus propinquus; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000378:metabolism; D005419:Flavonoids / Q000737:chemistry; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry* / Q000378:metabolism; D015203:Reproducibility of Results; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism; D020349:Surface Plasmon Resonance / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D055767:Immobilized Proteins; D012709:Serum Albumin; C027492:Huang Qi