title: A microfluidic system for cell type classification based on cellular size-independent electrical properties.
abstract: This paper presents a microfluidic system enabling cell type classification based on continuous characterization of size-independent electrical properties (e.g., specific membrane capacitance (C(specific membrane)) and cytoplasm conductivity (σ(cytoplasm)). In this study, cells were aspirated continuously through a constriction channel, while cell elongation and impedance profiles at two frequencies (1 kHz and 100 kHz) were measured simultaneously. Based on a proposed distributed equivalent circuit model, 1 kHz impedance data were used to evaluate cellular sealing properties with constriction channel walls and 100 kHz impedance data were translated to C(specific membrane) and σ(cytoplasm). Two lung cancer cell lines of CRL-5803 cells (n(cell) = 489) and CCL-185 cells (n(cell) = 487) were used to evaluate this technique, producing a C(specific membrane) of 1.63 ± 0.52 μF cm(-2) vs. 2.00 ± 0.60 μF cm(-2), and σ(cytoplasm) of 0.90 ± 0.19 S m(-1)vs. 0.73 ± 0.17 S m(-1). Neural network-based pattern recognition was used to classify CRL-5803 and CCL-185 cells, producing success rates of 65.4% (C(specific membrane)), 71.4% (σ(cytoplasm)), and 74.4% (C(specific membrane) and σ(cytoplasm)), suggesting that these two tumor cell lines can be classified based on their electrical properties.
authors: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing, PR China.
authors: Yang
authors: Y
authors: Zhao
authors: Deyong
authors: D
authors: Chen
authors: Yana
authors: Y
authors: Luo
authors: Hao
authors: H
authors: Li
authors: Bin
authors: B
authors: Deng
authors: Song-Bin
authors: SB
authors: Huang
authors: Tzu-Keng
authors: TK
authors: Chiu
authors: Min-Hsien
authors: MH
authors: Wu
authors: Rong
authors: R
authors: Long
authors: Hao
authors: H
authors: Hu
authors: Xiaoting
authors: X
authors: Zhao
authors: Wentao
authors: W
authors: Yue
authors: Junbo
authors: J
authors: Wang
authors: Jian
authors: J
authors: Chen
mesh_terms: D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000737:chemistry / Q000145:classification; D048429:Cell Size; D003593:Cytoplasm / Q000737:chemistry / Q000145:classification; D004553:Electric Conductivity; D005434:Flow Cytometry; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*