title: Exopolysaccharide produced by Pediococcus acidilactici M76 isolated from the Korean traditional rice wine, makgeolli.
abstract: This work is aimed to increase knowledge of the functional exopolysaccharide (EPS) from lactic acid bacteria (LAB) in makgeolli, a Korean fermented rice wine. Among LAB strains isolated from makgeolli, strain M76 was selected as a functional strain producing a bioactive EPS, based on its antioxidative activity on the DPPH radical. The 16S rRNA gene sequencing analysis showed a high sequence similarity (99.0%) with P. acidilactici, but had different biochemical properties with the already known P. acidilactici type strains in the aspect of carbohydrates utilization. The obtained P. acidilactici M76 produced a soluble EPS above 2 g/l. One-step chromatography using gel filtration after ethanol precipitation from the supernatant of P. acidilactici M76 was enough to obtain purified EPS with a single peak, showing a molecular mass of approximately 67 kDa. Componential and structural analyses of EPS by TLC, HPLC, and FT-IR indicated that the EPS is a glucan, consisting of glucose units. The purified EPS had antioxidant activity on the DPPH radical of 45.8% at a concentration of 1 mg/ml. The purified EPS also showed proliferative effect on the pancreatic RIN-m5F cell line and remarkable protection activity on alloxan-induced cytotoxicity. This potent antioxidant and antidiabetic EPS by LAB in makgeolli may contribute to understanding the functionality of makgeolli.
authors: Sunchang Research Center for Fermentation Microbes, Jeonbuk 595-804, Korea.
authors: Young-Ran
authors: YR
authors: Song
authors: Do-Youn
authors: DY
authors: Jeong
authors: Youn-Soo
authors: YS
authors: Cha
authors: Sang-Ho
authors: SH
authors: Baik
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics; D005285:Fermentation; D006801:Humans; D012275:Oryza / Q000382:microbiology*; D010376:Pediococcus / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014920:Wine / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D011135:Polysaccharides, Bacterial; D012336:RNA, Ribosomal, 16S