title: Tanycyte-like cells form a blood-cerebrospinal fluid barrier in the circumventricular organs of the mouse brain.
abstract: Tanycytes are highly specialized ependymal cells that form a blood-cerebrospinal fluid (CSF) barrier at the level of the median eminence (ME), a circumventricular organ (CVO) located in the tuberal region of the hypothalamus. This ependymal layer harbors well-organized tight junctions, a hallmark of central nervous system barriers that is lacking in the fenestrated portal vessels of the ME. The displacement of barrier properties from the vascular to the ventricular side allows the diffusion of blood-borne molecules into the parenchyma of the ME while tanycyte tight junctions control their diffusion into the CSF, thus maintaining brain homeostasis. In the present work, we combined immunohistochemical and permeability studies to investigate the presence of tanycyte barriers along the ventricular walls of other brain CVOs. Our data indicate that, unlike cuboidal ependymal cells, ependymal cells bordering the CVOs possess long processes that project into the parenchyma of the CVOs to reach the fenestrated capillary network. Remarkably, these tanycyte-like cells display well-organized tight junctions around their cell bodies. Consistent with these observations, permeability studies show that this ependymal layer acts as a diffusion barrier. Together, our results suggest that tanycytes are a characteristic feature of all CVOs and yield potential new insights into their involvement in regulating the exchange between the blood, the brain, and the CSF within these "brain windows."
authors: Laboratory of Development and Plasticity of the Postnatal Brain, Jean-Pierre Aubert Research Centre (JPARC), Inserm U837, University of Lille 2, Lille, 59000, France; NEUROBESE International Associated Laboratory, 59045, Lille cedex, France; Univ Lille Nord de France, School of Medicine, 59046, Lille cedex, France.
authors: Fanny
authors: F
authors: Langlet
authors: Amandine
authors: A
authors: Mullier
authors: Sebastien G
authors: SG
authors: Bouret
authors: Vincent
authors: V
authors: Prevot
authors: Benedicte
authors: B
authors: Dehouck
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry; D031608:Area Postrema / Q000502:physiology; D001812:Blood-Brain Barrier / Q000378:metabolism / Q000502:physiology*; D001921:Brain / Q000166:cytology / Q000502:physiology*; D002463:Cell Membrane Permeability; D002552:Cerebral Ventricles; D002555:Cerebrospinal Fluid / Q000502:physiology*; D063928:Ependymoglial Cells / Q000378:metabolism / Q000502:physiology*; D007150:Immunohistochemistry; D008297:Male; D008473:Median Eminence / Q000166:cytology / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D010539:Permeability; D013351:Subcommissural Organ / Q000502:physiology; D013356:Subfornical Organ / Q000502:physiology; D062725:Tight Junction Proteins / Q000378:metabolism
chemical_list: D000906:Antibodies; D062725:Tight Junction Proteins
keywords: area postrema; ependymocytes; organum vasculosum laminae terminalis; subcommissural organ; subfornical organ; tight junction protein
references: Int J Dev Neurosci. 1992 Oct;10(5):387-92
references: 1492590
references: Dev Dyn. 1995 Apr;202(4):325-32
references: 7626790
references: Ann N Y Acad Sci. 1988;529:31-9
references: 3293508
references: Microsc Res Tech. 2001 Mar 1;52(5):461-7
references: 11241857
references: J Cereb Blood Flow Metab. 2005 May;25(5):593-606
references: 15689955
references: Endocrinology. 2009 May;150(5):2283-91
references: 19179432
references: Cell Tissue Res. 1979 Sep 3;201(2):207-25
references: 509480
references: Cell Tissue Res. 1976 Oct 13;173(3):407-16
references: 991250
references: Cell Tissue Res. 2004 Aug;317(2):147-64
references: 15221441
references: Cell Metab. 2013 Apr 2;17(4):467-8
references: 23562070
references: FASEB J. 1993 May;7(8):678-86
references: 8500693
references: Int Rev Cytol. 1992;135:39-121
references: 1618609
references: Endocrinology. 2009 Dec;150(12):5509-19
references: 19837874
references: Neurobiol Dis. 2010 Jan;37(1):13-25
references: 19664713
references: Mol Cell Neurosci. 2009 Jul;41(3):337-47
references: 19409493
references: Brain Res Rev. 2007 Nov;56(1):119-47
references: 17659349
references: Microsc Res Tech. 1998 Apr 15;41(2):98-123
references: 9579598
references: Cell. 1984 Oct;38(3):779-89
references: 6386177
references: Eur J Cell Biol. 1986 Dec;42(2):288-94
references: 3545837
references: Cerebrospinal Fluid Res. 2008 Jan 24;5:3
references: 18218138
references: Front Neuroendocrinol. 2010 Jul;31(3):241-58
references: 20546773
references: Brain Res. 1981 Apr 6;210(1-2):253-60
references: 6784884
references: Cell Tissue Res. 1986;243(3):545-59
references: 2420461
references: Cell Metab. 2013 Apr 2;17(4):607-17
references: 23562080
references: J Comp Neurol. 2010 Apr 1;518(7):943-62
references: 20127760
references: Brain Res Mol Brain Res. 1994 Feb;21(3-4):235-46
references: 8170348
references: Glia. 1995 May;14(1):1-13
references: 7615341
references: Adv Biochem Psychopharmacol. 1981;28:303-20
references: 7010939
references: Arch Neurol. 1972 May;26(5):420-7
references: 4623695
references: Exp Biol Med (Maywood). 2007 Jan;232(1):14-26
references: 17202582
references: Lab Invest. 2005 Jun;85(6):734-46
references: 15908914
references: J Cereb Blood Flow Metab. 1987 Dec;7(6):663-72
references: 2891718
references: J Neuroendocrinol. 2002 Mar;14(3):247-55
references: 11999726
references: J Neurosci. 2004 Nov 17;24(46):10353-63
references: 15548649
references: J Comp Neurol. 1991 Sep 15;311(3):434-44
references: 1659591
references: Annu Rev Cell Dev Biol. 2006;22:207-35
references: 16771626
references: Neuroscience. 1998 May;84(1):177-91
references: 9522372
references: Neurosci Lett. 2001 Jul 13;307(2):77-80
references: 11427304
references: J Cell Biol. 2002 Mar 18;156(6):1099-111
references: 11889141
references: Nat Rev Neurosci. 2006 Jan;7(1):41-53
references: 16371949
references: Cell Tissue Res. 1989 Aug;257(2):255-62
references: 2570632
references: Neurochem Int. 2009 Sep;55(5):275-81
references: 19576514
references: J Cereb Blood Flow Metab. 2002 Jan;22(1):39-49
references: 11807392
references: J Chem Neuroanat. 2009 May;37(3):182-95
references: 19146948
references: Z Zellforsch Mikrosk Anat. 1973 Mar 7;137(4):493-512
references: 4348309
references: J Submicrosc Cytol Pathol. 1990 Apr;22(2):303-9
references: 2337891
references: J Comp Neurol. 2003 Jun 30;461(3):307-16
references: 12746870
references: Cell Tissue Res. 1975 Jul 16;160(3):315-26
references: 807331
references: J Cell Sci. 1997 Jul;110 ( Pt 14):1603-13
references: 9247194
references: Transplantation. 1989 Nov;48(5):874-7
references: 2683264
references: Int J Biochem Cell Biol. 2007;39(3):463-8
references: 17150405
references: Cell Tissue Res. 2012 Aug;349(2):589-603
references: 22584508
references: Neurosci Lett. 2011 Jan 7;487(2):187-90
references: 20951768
references: Cell Tissue Res. 1977 May 16;180(2):263-82
references: 194701
references: J Cell Biol. 1994 Jul;126(2):465-73
references: 8034745
references: Cell Tissue Res. 2003 Nov;314(2):251-62
references: 13680355
references: J Anat. 1994 Feb;184 ( Pt 1):35-58
references: 8157492
references: Int Rev Cytol. 2005;247:89-164
references: 16344112
references: J Comp Neurol. 1977 Feb 1;72(3):409-31
references: 833361
references: J Cell Sci. 1994 Jun;107 ( Pt 6):1529-43
references: 7962195
references: Annu Rev Physiol. 1998;60:161-77
references: 9558459
references: Cerebrospinal Fluid Res. 2005 Sep 30;2:8
references: 16197544
references: Neuropathology. 2013 Feb;33(1):17-29
references: 22537279
references: Cell Tissue Res. 1984;237(3):443-9
references: 6435877
references: Physiol Rev. 2008 Jul;88(3):983-1008
references: 18626065
references: Proc Natl Acad Sci U S A. 1983 Dec;80(23):7352-6
references: 6580650
references: J Comp Neurol. 2010 Feb 15;518(4):459-76
references: 20017211
references: J Cell Biol. 1988 Nov;107(5):1853-62
references: 2460470