title: Mitochondrial D-loop mutations and polymorphisms are connected with canine malignant cancers.
abstract: Abstract The aim of the conducted investigations was to identify differences in the D-loop nucleotide sequence between neoplastic tissue, normal tissue, and blood and to determine their correlation with the type of cancer in dogs. In 62.5% of the analyzed tumors of epithelial origin and 25% tumors of mesenchymal origin, substitution was detected within the D-loop sequence between the neoplastic tissue, normal tissue, and blood. Two mutations occurring in the carcinogenic process in position T15620C have been identified in epithelioma glandulae sebacei and carcinoma planoepithelialae keratodes. Blood and cancer heteroplasmy was diagnosed for carcinoma planoepithelialae keratodes and "Comedo" carcinoma. The results of the study indicate that polymorphic changes in the D-loop sequence promote carcinogenesis in dogs. Heteroplasmy diagnosed in blood and tumor cells and absence thereof in normal tissue may imply mtDNA recombination. High prevalence of mtDNA mutations in canine tumors may suggest mtDNA genetic instability, which is likely to play a role in carcinogenesis.
authors: Department of Biological Bases of Animal Production, University of Life Sciences in Lublin , Lublin , Poland .
authors: Brygida
authors: B
authors: Slaska
authors: Ludmila
authors: L
authors: Grzybowska-Szatkowska
authors: Magdalena
authors: M
authors: Surdyka
authors: Sylwia
authors: S
authors: Nisztuk
authors: Dorota
authors: D
authors: Rozanska
authors: Pawel
authors: P
authors: Rozanski
authors: Anna
authors: A
authors: Smiech
authors: Maciej
authors: M
authors: Orzelski
mesh_terms: D000818:Animals; D004252:DNA Mutational Analysis; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics*; D004283:Dog Diseases / Q000453:epidemiology / Q000235:genetics*; D004285:Dogs; D056726:Genetic Association Studies; D009154:Mutation; D009369:Neoplasms / Q000453:epidemiology / Q000235:genetics* / Q000662:veterinary; D009690:Nucleic Acid Conformation; D011110:Polymorphism, Genetic
chemical_list: D004272:DNA, Mitochondrial
keywords: Cancer; dog; heteroplasmy; mtDNA; recombination