title: Recruitment of Th1 effector cells in human tuberculosis: hierarchy of chemokine receptor(s) and their ligands.
abstract: Selective recruitment of IFN-γ biased Th1 effector cells at the pathologic site(s) determines the local immunity of tuberculosis (TB). We observed the enrichment of CXCR3, CCR5 and CD11a(high) T cells in the peripheral blood, pleural fluid and bronchoalveolar lavage of TB pleural effusion (TB-PE) and miliary tuberculosis (MTB) patients respectively. CXCR3(+)CCR5(+) T cells were significantly high at the local disease site(s) in both the forms of TB and their frequency was highest among activated lymphocytes in TB-PE. Interestingly, all CCR5(+) cells were invariably positive for CXCR3 but all CXCR3(+) cells did not co-express CCR5 in pleural fluid whereas the situation was reverse in bronchoalveolar lavage. These CXCR3(+)CCR5(+) cells dominantly produced IFN-γ in response to Mycobacterium tuberculosis antigen. In vitro chemotaxis assay indicates dominant role of RANTES and IP-10 in the selective recruitment of CXCR3(+)CCR5(+)cells at the tubercular pathologic sites.
authors: Department of Medicine, All India Institute of Medical Sciences, New Delhi, India.
authors: Pradip K
authors: PK
authors: Saha
authors: Department of Transplant Immunology and Immunogenetics, All India Institute of Medical Sciences, New Delhi, India.
authors: Prabhat K
authors: PK
authors: Sharma
authors: Department of Medicine, All India Institute of Medical Sciences, New Delhi, India. Electronic address: surensk@gmail.com.
authors: Surendra K
authors: SK
authors: Sharma
authors: Department of Transplant Immunology and Immunogenetics, All India Institute of Medical Sciences, New Delhi, India.
authors: Amar
authors: A
authors: Singh
authors: Department of Transplant Immunology and Immunogenetics, All India Institute of Medical Sciences, New Delhi, India. Electronic address: salilmitra1@yahoo.com.
authors: Dipendra K
authors: DK
authors: Mitra
mesh_terms: D000328:Adult; D040881:CD11a Antigen / Q000378:metabolism; D017252:CD3 Complex / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects; D018925:Chemokines / Q000494:pharmacology; D003710:Demography; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008024:Ligands; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology; D008297:Male; D010996:Pleural Effusion / Q000276:immunology / Q000473:pathology; D054397:Receptors, CCR3 / Q000378:metabolism*; D019713:Receptors, CCR5 / Q000378:metabolism*; D018417:Th1 Cells / Q000378:metabolism*; D014376:Tuberculosis / Q000276:immunology* / Q000473:pathology; D014391:Tuberculosis, Miliary / Q000276:immunology / Q000473:pathology
chemical_list: C515350:CCR3 protein, human; C584933:CCR5 protein, human; D040881:CD11a Antigen; D017252:CD3 Complex; D018925:Chemokines; D008024:Ligands; D054397:Receptors, CCR3; D019713:Receptors, CCR5; D015847:Interleukin-4; D007371:Interferon-gamma; D017493:Leukocyte Common Antigens