title: Intracellular SERS nanoprobes for distinction of different neuronal cell types.
abstract: Distinction between closely related and morphologically similar cells is difficult by conventional methods especially without labeling. Using nuclear-targeted gold nanoparticles (AuNPs) as intracellular probes we demonstrate the ability to distinguish between progenitor and differentiated cell types in a human neuroblastoma cell line using surface-enhanced Raman spectroscopy (SERS). SERS spectra from the whole cell area as well as only the nucleus were analyzed using principal component analysis that allowed unambiguous distinction of the different cell types. SERS spectra from the nuclear region showed the developments during cellular differentiation by identifying an increase in DNA/RNA ratio and proteins transcribed. Our approach using nuclear-targeted AuNPs and SERS imaging provides label-free and noninvasive characterization that can play a vital role in identifying cell types in biomedical stem cell research.
authors: Sector for Biological and Soft Systems, Cavendish Laboratory, Department of Physics, University of Cambridge, 19 JJ Thomson Avenue, Cambridge, CB3 0HE, United Kingdom.
authors: Anna
authors: A
authors: Huefner
authors: Wei-Li
authors: WL
authors: Kuan
authors: Roger A
authors: RA
authors: Barker
authors: Sumeet
authors: S
authors: Mahajan
mesh_terms: D045744:Cell Line, Tumor; D004247:DNA / Q000378:metabolism; D006801:Humans; D015335:Molecular Probes; D036103:Nanotechnology; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D012313:RNA / Q000378:metabolism; D013059:Spectrum Analysis, Raman
chemical_list: D015335:Molecular Probes; D011506:Proteins; D012313:RNA; D004247:DNA
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