title: A novel approach to capillary plasma microsampling for quantitative bioanalysis.
abstract: BACKGROUND
  A novel device and procedure for the collection and isolation of microvolumes of plasma have been developed and two pilot rodent PK studies have been completed.
  
  
  RESULTS
  This method involves collection of blood into a plastic-wrapped, EDTA-coated capillary tube, containing a small amount of a thixotropic gel and a porous plug. Following blood collection, the capillary is placed into a secondary labeled container suitable for centrifugation and plasma is generated. During centrifugation, the thixotropic gel isolates the plasma from the red blood cells and creates a physical barrier between the two matrices. The plasma is then dispensed from the capillary tube into a separate container for storage or processing.
  
  
  CONCLUSIONS
  A simple and robust novel approach for the collection of small plasma volumes from rodent TK studies has been demonstrated.
authors: Bioanalytical Science & Toxicokinetics, DMPK, Platform Technology & Science, GlaxoSmithKline Pharmaceuticals, 709 Swedeland Road, King of Prussia, PA 19406, USA. chester.l.bowen@gsk.com
authors: Chester L
authors: CL
authors: Bowen
authors: Hermes
authors: H
authors: Licea-Perez
authors: Molly Z
authors: MZ
authors: Karlinsey
authors: Kristen
authors: K
authors: Jurusik
authors: Esaie
authors: E
authors: Pierre
authors: Joseph
authors: J
authors: Siple
authors: Jim
authors: J
authors: Kenney
authors: Alan
authors: A
authors: Stokes
authors: Neil
authors: N
authors: Spooner
authors: Christopher A
authors: CA
authors: Evans
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000295:instrumentation / Q000379:methods*; D001800:Blood Specimen Collection / Q000295:instrumentation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D051381:Rats; D017208:Rats, Wistar; D053719:Tandem Mass Spectrometry