title: Self-assembled, aptamer-tethered DNA nanotrains for targeted transport of molecular drugs in cancer theranostics.
abstract: Nanotechnology has allowed the construction of various nanostructures for applications, including biomedicine. However, a simple target-specific, economical, and biocompatible drug delivery platform with high maximum tolerated doses is still in demand. Here, we report aptamer-tethered DNA nanotrains (aptNTrs) as carriers for targeted drug transport in cancer therapy. Long aptNTrs were self-assembled from only two short DNA upon initiation by modified aptamers, which worked like locomotives guiding nanotrains toward target cancer cells. Meanwhile, tandem "boxcars" served as carriers with high payload capacity of drugs that were transported to target cells and induced selective cytotoxicity. aptNTrs enhanced maximum tolerated dose in nontarget cells. Potent antitumor efficacy and reduced side effects of drugs delivered by biocompatible aptNTrs were demonstrated in a mouse xenograft tumor model. Moreover, fluorophores on nanotrains and drug fluorescence dequenching upon release allowed intracellular signaling of nanotrains and drugs. These results make aptNTrs a promising targeted drug transport platform for cancer theranostics.
authors: Molecular Science and Biomedicine Laboratory, College of Biology and College of Chemistry and Chemical Engineering, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha 410082, China.
authors: Guizhi
authors: G
authors: Zhu
authors: Jing
authors: J
authors: Zheng
authors: Erqun
authors: E
authors: Song
authors: Michael
authors: M
authors: Donovan
authors: Kejing
authors: K
authors: Zhang
authors: Chen
authors: C
authors: Liu
authors: Weihong
authors: W
authors: Tan
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004247:DNA / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers / Q000737:chemistry; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D051379:Mice; D016513:Mice, SCID; D053761:Nanocomposites / Q000737:chemistry; D050997:Nanomedicine / Q000379:methods*; D009369:Neoplasms / Q000378:metabolism / Q000628:therapy*; D011485:Protein Binding; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D052157:Aptamers, Nucleotide; D001672:Biocompatible Materials; D004337:Drug Carriers; D004247:DNA
keywords: DNA nanomedicines; in vivo; self-assembly; targeted anticancer drug delivery
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