title: Function of ezrin-radixin-moesin proteins in migration of subventricular zone-derived neuroblasts following traumatic brain injury.
abstract: Throughout life, newly generated neuroblasts from the subventricular zone migrate toward the olfactory bulb through the rostral migratory stream. Upon brain injury, these migrating neuroblasts change their route and begin to migrate toward injured regions, which is one of the regenerative responses after brain damage. This injury-induced migration is triggered by stromal cell-derived factor 1 (SDF1) released from microglia near the damaged site; however, it is still unclear how these cells transduce SDF1 signals and change their direction. In this study, we found that SDF1 promotes the phosphorylation of ezrin-radixin-moesin (ERM) proteins, which are key molecules in organizing cell membrane and linking signals from the extracellular environment to the intracellular actin cytoskeleton. Blockade of ERM activation by overexpressing dominant-negative ERM (DN-ERM) efficiently perturbed the migration of neuroblasts. Considering that DN-ERM-expressing neuroblasts failed to maintain proper migratory cell morphology, it appears that ERM-dependent regulation of cell shape is required for the efficient migration of neuroblasts. These results suggest that ERM activation is an important step in the directional migration of neuroblasts in response to SDF1-CXCR4 signaling following brain injury.
authors: Department of Anatomy and Division of Brain Korea 21 Biomedical Science, Korea University College of Medicine, Seoul, Korea; Brain and Neuroendocrine Laboratory, School of Biological Sciences, College of Natural Sciences, Seoul National University, Seoul, Korea.
authors: Younghye
authors: Y
authors: Moon
authors: Joo Yeon
authors: JY
authors: Kim
authors: Woon Ryoung
authors: WR
authors: Kim
authors: Hyun Jung
authors: HJ
authors: Kim
authors: Min Jee
authors: MJ
authors: Jang
authors: Yoonkey
authors: Y
authors: Nam
authors: Kyungjin
authors: K
authors: Kim
authors: Hyun
authors: H
authors: Kim
authors: Woong
authors: W
authors: Sun
mesh_terms: D000818:Animals; D001930:Brain Injuries / Q000378:metabolism* / Q000473:pathology; D002454:Cell Differentiation / Q000502:physiology; D048708:Cell Growth Processes / Q000502:physiology; D002465:Cell Movement / Q000502:physiology*; D002552:Cerebral Ventricles / Q000378:metabolism* / Q000473:pathology; D003598:Cytoskeletal Proteins / Q000378:metabolism*; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008840:Microfilament Proteins / Q000378:metabolism*; D058953:Neural Stem Cells / Q000378:metabolism* / Q000473:pathology; D009830:Olfactory Bulb / Q000378:metabolism / Q000473:pathology; D010766:Phosphorylation; D019718:Receptors, CXCR4 / Q000378:metabolism; D000099149:Ezrin
chemical_list: D003598:Cytoskeletal Proteins; D008565:Membrane Proteins; D008840:Microfilament Proteins; D019718:Receptors, CXCR4; D000099149:Ezrin; C071058:moesin; C071776:radixin
keywords: Brain injury; ERM proteins; Migration; Phosphorylation; SDF1-CXCR4