title: Heterozygous alleles restore male fertility to cytoplasmic male-sterile radish (Raphanus sativus L.): a case of overdominance.
abstract: The practice of hybridization has greatly contributed to the increase in crop productivity. A major component that exploits heterosis in crops is the cytoplasmic male sterility (CMS)/nucleus-controlled fertility restoration (Rf) system. Through positional cloning, it is shown that heterozygous alleles (RsRf3-1/RsRf3-2) encoding pentatricopeptide repeat (PPR) proteins are responsible for restoring fertility to cytoplasmic male-sterile radish (Raphanus sativus L.). Furthermore, it was found that heterozygous alleles (RsRf3-1/RsRf3-2) show higher expression and RNA polymerase II occupancy in the CMS cytoplasmic background compared with their homozygous alleles (RsRf3-1/RsRf3-1 or RsRf3-2/RsRf3-2). These data provide new insights into the molecular mechanism of fertility restoration to cytoplasmic male-sterile plants and illustrate a case of overdominance.
authors: CAS Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, PR China.
authors: Zhi Wei
authors: ZW
authors: Wang
authors: Chuan
authors: C
authors: De Wang
authors: Chuan
authors: C
authors: Wang
authors: Lei
authors: L
authors: Gao
authors: Shi Yong
authors: SY
authors: Mei
authors: Yuan
authors: Y
authors: Zhou
authors: Chang Ping
authors: CP
authors: Xiang
authors: Ting
authors: T
authors: Wang
mesh_terms: D000483:Alleles; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D005799:Genes, Dominant; D006579:Heterozygote; D051479:Plant Infertility / Q000235:genetics* / Q000502:physiology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D031224:Raphanus / Q000235:genetics*
chemical_list: D010940:Plant Proteins
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