title: Effects of single and combined genotypes of MC4R and POU1F1 genes on two production traits in Langshan chicken.
abstract: The objective of this study was to analyze the effects of single and combined genotypes of MC4R and POU1F1 genes in Chinese well-known indigenous chicken (Langshan chicken) population. Genetic variants within MC4R gene and POU1F1 gene were screened through PCR-SSCP and DNA sequencing methods. A C/T mutation at nt 944 in MC4R gene (NC_006089.2:g. 944C>T) and a G/A mutation at nt 3109 in POU1F1 gene (NC_006088.2:g. 3109 G>A) were identified. Associations between the mutations of the two genes with two production traits were analyzed. The results showed that, at MC4R locus, individuals with BB and AB genotypes had highly significantly higher body weight at 16 weeks (p < 0.01) than did those with the AA genotype. And, individuals within AA and AB genotypes had significantly higher egg numbers at 300 days (p < 0.05). At POU1F1 locus, individuals with CD genotype had higher body weight at 16 weeks and egg numbers at 300 days (p < 0.05). Furthermore, combined genotypes from these two loci were found to be associated with egg numbers at 300 days (p < 0.05). The individuals within combined genotype AB/CD had higher egg production. Therefore, variations identified within the MC4R and POU1F1genes are suitable for future use in identifying chickens with the genetic potential of higher body weight and reproductive traits, at least in the population of Langshan chickens.
authors: Institute of Cellular and Molecular Biology, College of Life Science, Xuzhou Normal University, Xuzhou, 221116, Jiangsu, People's Republic of China. ylj_23@163.com
authors: Lin Jun
authors: LJ
authors: Yan
authors: Xing Tang
authors: XT
authors: Fang
authors: Yao
authors: Y
authors: Liu
authors: Chun Lei
authors: CL
authors: Zhang
authors: Xuan Xuan
authors: XX
authors: Liu
authors: Jing
authors: J
authors: Zhao
authors: Jing Jing
authors: JJ
authors: Li
authors: Hong
authors: H
authors: Chen
mesh_terms: D000483:Alleles; D000818:Animals; D001835:Body Weight / Q000235:genetics; D002645:Chickens / Q000235:genetics*; D004531:Eggs; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D005838:Genotype; D019655:Quantitative Trait, Heritable; D044105:Receptor, Melanocortin, Type 4 / Q000235:genetics*; D012098:Reproduction / Q000235:genetics*; D050817:Transcription Factor Pit-1 / Q000235:genetics*
chemical_list: D044105:Receptor, Melanocortin, Type 4; D050817:Transcription Factor Pit-1
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