title: miR-30a downregulation aggravates pressure overload-induced cardiomyocyte hypertrophy.
abstract: miRNAs play an important role in the pathogenesis of cardiac hypertrophy and dysfunction. However, little is known about how miR-30a regulates cardiomyocyte hypertrophy. In the study, Male C57BL/6 mice were subjected to thoracic aortic constriction, and hearts were harvested at 3 weeks. We assayed miR-30a expression level by real-time PCR and defined the molecular mechanisms of miR-30a-mediated cardiomyocyte hypertrophy. We found that myocardial expression of miR-30a was decreased in mouse models of hypertrophy and in H9c2 cells treated with phenylephrine. MiR-30a inhibition markedly increased mRNA expression of cardiac hypertrophy markers such as atrial natriuretic factor and brain natriuretic peptide in H9c2, and cell size was increased after miR-30a inhibitor treatment. Downregulated miR-30a activated autophagy by inhibiting beclin-1 expression in H9c2 cell. More important, autophagy inhibition suppressed miR-30a inhibitor-induced cardiomyocyte hypertrophy. Together, our data demonstrated that downregulated miR-30a aggravates pressure overload-induced cardiomyocyte hypertrophy by activating autophagy, thus offering a new target for the therapy of cardiomyocyte hypertrophy.
authors: Department of Emergency Medicine, The Forth Affiliated Hospital, Harbin Medical University, Harbin, People's Republic of China.
authors: Xuesong
authors: X
authors: Yin
authors: Chenghai
authors: C
authors: Peng
authors: Wenhu
authors: W
authors: Ning
authors: Chunyan
authors: C
authors: Li
authors: Zhongqiao
authors: Z
authors: Ren
authors: Jihong
authors: J
authors: Zhang
authors: Han
authors: H
authors: Gao
authors: Kan
authors: K
authors: Zhao
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000235:genetics / Q000378:metabolism; D001343:Autophagy; D006332:Cardiomegaly / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D002460:Cell Line; D048429:Cell Size; D015536:Down-Regulation; D005786:Gene Expression Regulation; D057809:HEK293 Cells; D006801:Humans; D006973:Hypertension / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D032383:Myocytes, Cardiac / Q000378:metabolism / Q000473:pathology; D020097:Natriuretic Peptide, Brain / Q000235:genetics / Q000378:metabolism; D051381:Rats
chemical_list: D020413:3' Untranslated Regions; D035683:MicroRNAs; C534715:Mirn30d microRNA, mouse; D020097:Natriuretic Peptide, Brain; D009320:Atrial Natriuretic Factor
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