title: Proteomic analysis of the cerebrospinal fluid of Parkinson's disease patients pre- and post-deep brain stimulation.
abstract: OBJECTIVE
  To investigate alterations in protein expression associated with deep brain stimulation (DBS) in an attempt to elucidate possible mechanisms of action .
  
  
  METHODS
  Cerebrospinal fluid (CSF), obtained from six Parkinson's disease (PD) patients (pre- and post-DBS) and from six normal healthy controls, was studied for differentially expressed proteins. 2-D DIGE, in combination with MALDI-TOF and TOF-TOF Mass Spectrometry (MS) or ESI-MS, was used to identify the changed proteins (3 PD patients and 3 controls). Selected proteins were further studied using western blotting (6 PD patients and 6 controls).
  
  
  RESULTS
  Twenty-one proteins were identified after MS and protein database interrogation. Apart from apolipoprotein A-I (apoA-I), the expression levels of complement C4 (C4), IgA, tetranectin, and extracellular superoxide dismutase (EC-SOD), detected by western blotting, correlated well with the 2-D DIGE results. In the follow-up period, the expression levels of C4, apoA-I and IgA were stable whereas EC-SOD and tetranectin were significantly elevated. In addition, when DBS was ceased in one patient due to a suicide attempt, the levels of EC-SOD and tetranectin significantly decreased.
  
  
  CONCLUSIONS
  Our preliminary results suggest that variations in the expression levels of EC-SOD and tetranectin in CSF is related to DBS.
authors: Department of Neurosurgery, Jinshan Hospital, Fudan University, Shanghai, China. weswes2010@163.com
authors: Er-Song
authors: ES
authors: Wang
authors: Hui-Bin
authors: HB
authors: Yao
authors: Ying-Hui
authors: YH
authors: Chen
authors: Gan
authors: G
authors: Wang
authors: Wen-Wei
authors: WW
authors: Gao
authors: Yi-Rui
authors: YR
authors: Sun
authors: Ji-Guang
authors: JG
authors: Guo
authors: Jian-Wen
authors: JW
authors: Hu
authors: Cheng-Chuan
authors: CC
authors: Jiang
authors: Jin
authors: J
authors: Hu
mesh_terms: D000368:Aged; D030562:Databases, Protein; D046690:Deep Brain Stimulation; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010300:Parkinson Disease / Q000134:cerebrospinal fluid / Q000628:therapy*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D020543:Proteome