title: Process parameters for decolorization and biodegradation of orange II (Acid Orange 7) in dye-simulated minimal salt medium and subsequent textile effluent treatment by Bacillus cereus (MTCC 9777) RMLAU1.
abstract: In this study, Bacillus cereus isolate from tannery effluent was employed for orange II dye decolorization in simulated minimal salt broth and textile effluent. Most of the physicochemical parameters of textile effluent were above the permissible limits. The strain was highly tolerant to dye up to 500 mg l(-1). Increasing dye concentration exerted inhibitory effect on the bacterial growth and decolorization. The maximum decolorization of initial 100 mg dye l(-1) was achieved at optimum pH 8.0 and 33 °C under static culture conditions during 96-h incubation. Supplementation with optimized glucose (0.4%, w/v) and ammonium sulfate (0.1%, w/v) with 3.0% B. cereus inoculum further enhanced dye decolorization to highest 68.5% within 96-h incubation. A direct correlation was evident between bacterial growth and dye decolorization. Under above optimized conditions, 24.3% decolorization of unsterilized real textile effluent by native microflora was achieved. The effluent decolorization enhanced substantially to 37.1% with B. cereus augmentation and to 40.5% when supplemented with glucose and ammonium sulfate without augmentation. The maximum decolorization of 52.5% occurred when textile effluent was supplemented with optimized exogenous carbon and nitrogen sources along with B. cereus augmentation. Gas chromatography-mass spectrometry identified sulfanilic acid as orange II degradation product. Fourier transform infra red spectroscopy of metabolic products indicated the presence of amino and hydroxyl functional groups. This strain may be suitably employed for in situ decolorization of textile industrial effluent under broad environmental conditions.
authors: Centre of Excellence, DST-FIST Supported Department of Microbiology, Dr. Ram Manohar Lohia Avadh University, Faizabad, 224001, India, sk_garg001@yahoo.com.
authors: Satyendra Kumar
authors: SK
authors: Garg
authors: Manikant
authors: M
authors: Tripathi
mesh_terms: D001391:Azo Compounds / Q000032:analysis / Q000378:metabolism*; D001409:Bacillus cereus / Q000378:metabolism*; D001557:Benzenesulfonates / Q000032:analysis / Q000378:metabolism*; D001673:Biodegradation, Environmental; D007220:Industrial Waste / Q000032:analysis*; D009584:Nitrogen / Q000378:metabolism; D012965:Sodium Chloride / Q000378:metabolism; D013633:Tanning; D013783:Textile Industry; D013784:Textiles; D014865:Waste Disposal, Fluid / Q000379:methods; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000378:metabolism*
chemical_list: D001391:Azo Compounds; D001557:Benzenesulfonates; D007220:Industrial Waste; D014874:Water Pollutants, Chemical; D012965:Sodium Chloride; D009584:Nitrogen; C014722:2-naphthol orange
references: J Ind Microbiol Biotechnol. 1999 Jul;23(1):686-90
references: 10455502
references: Appl Environ Microbiol. 1992 Aug;58(8):2397-401
references: 1514787
references: Bioresour Technol. 2007 Aug;98(11):2082-8
references: 17055263
references: Biodegradation. 2007 Jun;18(3):311-6
references: 17004031
references: Eur J Biochem. 1982 Dec;129(1):197-203
references: 7160382
references: Indian J Microbiol. 2011 Jan;51(1):61-9
references: 22282630
references: Indian J Environ Health. 2002 Oct;44(4):320-8
references: 13677071
references: J Environ Biol. 2008 Nov;29(6):831-6
references: 19297974
references: Braz J Microbiol. 2010 Oct;41(4):907-15
references: 24031570
references: Bioresour Technol. 2002 May;82(3):225-31
references: 11991070
references: Indian J Microbiol. 2011 Oct;51(4):501-8
references: 23024414
references: Biotechnol Bioeng. 2006 Mar 5;93(4):631-5
references: 16411245