title: [Temperature-switched high-efficiency D-lactate production from glycerol].
abstract: Glycerol from oil hydrolysis industry is being considered as one of the abundent raw materials for fermentation industry. In present study, the aerobic and anaerobic metabolism and growth properties on glycerol by Esherichia coli CICIM B0013-070, a D-lactate over-producing strain constructed previously, at different temperatures were investigated, followed by a novel fermentation process, named temperature-switched process, was established for D-lactate production from glycerol. Under the optimal condition, lactate yield was increased from 64.0% to 82.6%. Subsequently, the yield of D-lactate from glycerol was reached up to 88.9% while a thermo-inducible promoter was used to regulate D-lactate dehydrogenase transcription.
authors: School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China.
authors: Kangming
authors: K
authors: Tian
authors: Li
authors: L
authors: Zhou
authors: Xianzhong
authors: X
authors: Chen
authors: Wei
authors: W
authors: Shen
authors: Guiyang
authors: G
authors: Shi
authors: Suren
authors: S
authors: Singh
authors: Fuping
authors: F
authors: Lu
authors: Zhengxiang
authors: Z
authors: Wang
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D005285:Fermentation; D005990:Glycerol / Q000378:metabolism*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D019344:Lactic Acid / Q000096:biosynthesis*; D011401:Promoter Regions, Genetic / Q000235:genetics; D013696:Temperature
chemical_list: D019344:Lactic Acid; D007770:L-Lactate Dehydrogenase; D005990:Glycerol