title: Intrathecal glutamate release during hindlimb tourniquet inflation and femoral artery occlusion in rats.
abstract: OBJECTIVE
  A tourniquet is commonly used in limb surgery. Tourniquet inflation after a period of time may produce painful sensation. While the mechanisms of tourniquet-induced pain are still unknown, two components, pressure and ischemia, have been proposed. In this study, in vivo microdialysis was used to detect changes in intrathecal glutamate, an excitatory amino acid highly relevant to pain transmission, following hindlimb tourniquet application and femoral artery occlusion in the rat.
  
  
  METHODS
  Male Wistar rats were used. For the tourniquet study, 6 rats of the study group received 30 minutes right hindlimb tourniquet inflation and another 6 rats as the control group received only tourniquet application without inflation. In the femoral artery occlusion study, 6 rats of the study group received 30 minutes right femoral artery occlusion and another 6 rats as the control group received only sham operation without femoral artery occlusion. Cerebrospinal fluid dialysates were collected prior to, during, and after tourniquet application or femoral artery occlusion. Glutamate was measured by HPLC.
  
  
  RESULTS
  A significant increase in intrathecal glutamate release was found during the tourniquet inflation period, and it returned to baseline after tourniquet deflation. No change of glutamate release was noted during femoral artery occlusion or after femoral artery reperfusion.
  
  
  CONCLUSIONS
  The intrathecal glutamate release was increased by the hindlimb tourniquet inflation, but not influenced by femoral artery occlusion in the rat.
authors: Department of Anesthesiology, Tri-Service General Hospital and National Defense Medical Center, Taipei, Taiwan, R.O.C. cherng1018@yahoo.com.tw
authors: Chen-Hwan
authors: CH
authors: Cherng
authors: Chih-Shung
authors: CS
authors: Wong
authors: Ching-Tang
authors: CT
authors: Wu
authors: Chun-Chang
authors: CC
authors: Yeh
mesh_terms: D000818:Animals; D001157:Arterial Occlusive Diseases / Q000378:metabolism*; D005263:Femoral Artery; D018698:Glutamic Acid / Q000134:cerebrospinal fluid / Q000378:metabolism*; D006614:Hindlimb / Q000098:blood supply*; D008297:Male; D017551:Microdialysis; D010146:Pain / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013116:Spinal Cord / Q000378:metabolism*; D014111:Tourniquets
chemical_list: D018698:Glutamic Acid