title: The value of MMP-9 for breast and non-small cell lung cancer patients' survival.
abstract: OBJECTIVE
  Matrix metalloproteinases (MMPs) are implicated in cancer cells invasion and metastasis processes and have been investigated as potential cancer biomarkers. In this study MMP-9 gene expression and MMP-9 -1562 C/T polymorphism in breast and non-small cell lung cancer patients' blood and tumor samples and its correlation with clinicopathological parameters were investigated.
  
  
  METHODS
  MMP-9 gene expression was assessed by reverse transcription - polymerase chain reaction method in 108 cancer patients' blood and tumor samples. MMP-9 -1562 C/T polymorphism was determined by the polymerase chain reaction - based restriction fragment length polymorphism method.
  
  
  RESULTS
  Significant relationship of MMP-9 gene expression and tumor differentiation grade was found only between groups with G1 and G3 breast tumors. Low survival rates were identified among positive MMP-9 expression in blood and ductal carcinoma of the breast (p=0.01) and negative progesterone receptor reaction (p=0.04). Significant differences in the distribution among genotypes were found between groups with stage I and stages III/IV (p=0.005) as well as between groups with lymph node status N0 and N1 (p<0.001). Breast cancer patients with tumor differentiation grade G3 and identified CC variant had a longer survival time (p=0.014). Shorter survival time was found among positive MMP-9 expression in tumor and stage I non-small cell lung cancer patients with negative lymph node (p=0.012) and squamous cell carcinoma (p=0.019).
  
  
  CONCLUSIONS
  Expression of MMP-9 in blood and tumor together indicates worse prognosis for breast cancer patients.
authors: Institute of Oncology, Vilnius University, Vilnius, Lithuania. Diana.Schveigert@vuoi.lt
authors: D
authors: D
authors: Schveigert
authors: S
authors: S
authors: Cicenas
authors: S
authors: S
authors: Bruzas
authors: N E
authors: NE
authors: Samalavicius
authors: Z
authors: Z
authors: Gudleviciene
authors: J
authors: J
authors: Didziapetriene
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood; D001943:Breast Neoplasms / Q000097:blood* / Q000401:mortality; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood* / Q000401:mortality; D002454:Cell Differentiation; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D005838:Genotype; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood* / Q000401:mortality; D020780:Matrix Metalloproteinase 9 / Q000097:blood*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D011379:Prognosis; D011980:Receptors, Progesterone / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D011980:Receptors, Progesterone; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9