title: Molecular mapping of a sunflower rust resistance gene from HAR6.
abstract: Sunflower rust, caused by Puccinia helianthi Schw., can result in significant yield losses in cultivated sunflower (Helianthus annuus L. var. macrocarpus Ckll.). HAR6 is a germplasm population resistant to most predominant rust races. The objectives of this study were to map the resistance factor present in HAR6 (R HAR6 ), and to provide and validate molecular tools for the identification of this gene for marker assisted selection purposes. Virulence reaction of seedlings for the F2 population and F2:3 families suggested that a single dominant gene confers rust resistance in HAR6-1, a selected rust resistance line from the original population. Genetic mapping with eight markers covered 97.4 cM of genetic distance on linkage group 13 of the sunflower consensus map. A co-dominant marker ZVG61 is the closest marker distal to R HAR6 at a genetic distance of 0.7 cM, while ORS581, a dominant marker linked in the coupling phase, is proximal to R HAR6 at a genetic distance of 1.5 cM. Validation of these markers was assessed by converting a susceptible line into a rust resistant isoline by means of marker assisted backcrossing. The application of these results to assist the breeding process and to design new strategies for rust control in sunflower is discussed.
authors: Department of Biotechnology, Nidera S.A. , Casilla de Correo 6, CP.: 2600 Venado Tuerto, Santa Fe, Argentina.
authors: Mariano
authors: M
authors: Bulos
authors: María L
authors: ML
authors: Ramos
authors: Emiliano
authors: E
authors: Altieri
authors: Carlos A
authors: CA
authors: Sala
keywords: Puccinia helianthi; disease resistance; marker assisted backcrossing; molecular breeding; sunflower rust
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