title: Differential regulation of Arabidopsis plastid gene expression and RNA editing in non-photosynthetic tissues.
abstract: RNA editing is one of the post-transcriptional processes that commonly occur in plant plastids and mitochondria. In Arabidopsis, 34 C-to-U RNA editing events, affecting transcripts of 18 plastid genes, have been identified. Here, we examined the editing and expression of these transcripts in different organs, and in green and non-green seedlings (etiolated, cia5-2, ispF and ispG albino mutants, lincomycin-, and norflurazon-treated). The editing efficiency of Arabidopsis plastid transcripts varies from site to site, and may be specifically regulated in different tissues. Steady state levels of plastid transcripts are low or undetectable in etiolated seedlings, but most editing sites are edited with efficiencies similar to those observed in green seedlings. By contrast, the editing of some sites is completely lost or significantly reduced in other non-green tissues; for instance, the editing of ndhB-149, ndhB-1255, and ndhD-2 is completely lost in roots and in lincomycin-treated seedlings. The editing of ndhD-2 is also completely lost in albino mutants and norflurazon-treated seedlings. However, matK-640 is completely edited, and accD-794, atpF-92, psbE-214, psbF-77, psbZ-50, and rps14-50 are completely or highly edited in both green and non-green tissues. In addition, the expression of nucleus-encoded RNA polymerase dependent transcripts is specifically induced by lincomycin, and the splicing of ndhB transcripts is significantly reduced in the albino mutants and inhibitor-treated seedlings. Our results indicate that plastid gene expression, and the splicing and editing of plastid transcripts are specifically and differentially regulated in various types of non-green tissues.
authors: Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan.
authors: Ching-Chih
authors: CC
authors: Tseng
authors: Chih-Jen
authors: CJ
authors: Lee
authors: Yi-Ting
authors: YT
authors: Chung
authors: Tzu-Ying
authors: TY
authors: Sung
authors: Ming-Hsiun
authors: MH
authors: Hsieh
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics*; D060365:Chloroplast Proteins / Q000235:genetics; D003573:Cytochrome b Group / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000235:genetics; D008034:Lincomycin / Q000494:pharmacology; D009245:NADH Dehydrogenase / Q000235:genetics; D045332:Photosystem II Protein Complex / Q000235:genetics; D018087:Plastids / Q000235:genetics* / Q000378:metabolism; D017393:RNA Editing / Q000235:genetics* / Q000502:physiology; D018749:RNA, Plant / Q000235:genetics*
chemical_list: D029681:Arabidopsis Proteins; D060365:Chloroplast Proteins; D003573:Cytochrome b Group; D045332:Photosystem II Protein Complex; C479885:PsbD protein, Arabidopsis; C501403:PsbE protein, Arabidopsis; D018749:RNA, Plant; C079008:ndhB protein, Arabidopsis; D008034:Lincomycin; D009245:NADH Dehydrogenase
references: J Mol Evol. 2001 Oct-Nov;53(4-5):327-32
references: 11675592
references: Plant Physiol. 2009 Jul;150(3):1260-71
references: 19448041
references: Curr Genet. 1998 May;33(5):340-4
references: 9618584
references: Plant Mol Biol. 1996 Feb;30(3):667-72
references: 8605316
references: DNA Res. 1999 Oct 29;6(5):283-90
references: 10574454
references: EMBO J. 1993 Feb;12(2):563-71
references: 8440246
references: EMBO J. 1997 Jul 1;16(13):4041-8
references: 9233813
references: Plant Mol Biol. 1997 Jan;33(1):79-86
references: 9037161
references: Plant J. 2009 Apr;58(1):82-96
references: 19054358
references: Plant Cell. 1994 Oct;6(10):1455-65
references: 7994178
references: EMBO J. 1994 Oct 3;13(19):4623-8
references: 7925303
references: Plant J. 2008 Nov;56(4):590-602
references: 18657233
references: Curr Genet. 2002 Apr;41(1):48-52
references: 12073100
references: RNA. 2009 Jun;15(6):1142-53
references: 19395655
references: Plant Mol Biol. 2010 Jun;73(3):309-23
references: 20143129
references: Proc Natl Acad Sci U S A. 2012 May 29;109(22):E1453-61
references: 22566615
references: Mol Gen Genet. 1998 Feb;257(3):299-307
references: 9520264
references: Plant J. 2010 Jan;61(2):339-49
references: 19845878
references: Proc Natl Acad Sci U S A. 2007 May 8;104(19):8178-83
references: 17483454
references: Plant Physiol. 2009 Aug;150(4):2030-44
references: 19493969
references: J Plant Physiol. 2004 Jan;161(1):113-5
references: 15002671
references: Plant Cell. 2008 Apr;20(4):856-74
references: 18441214
references: Plant Physiol. 2005 Jun;138(2):641-53
references: 15863698
references: J Exp Bot. 2012 Jan;63(1):251-60
references: 21926093
references: Nucleic Acids Res. 2008 Feb;36(3):785-92
references: 18084023
references: Nucleic Acids Res. 1998 Mar 1;26(5):1185-90
references: 9469825
references: EMBO J. 1993 Nov;12(11):4291-6
references: 8223439
references: Plant Cell. 2006 Sep;18(9):2247-57
references: 16891400
references: Annu Rev Plant Biol. 2010;61:125-55
references: 20192740
references: Trends Plant Sci. 2008 Dec;13(12):663-70
references: 19004664
references: Mol Gen Genet. 1999 Oct;262(3):462-7
references: 10589833
references: J Mol Biol. 1995 Sep 1;251(5):614-28
references: 7666415
references: Mol Genet Genomics. 2007 Jun;277(6):725-34
references: 17333279
references: Plant Cell. 2009 Jan;21(1):146-56
references: 19182104
references: Mol Gen Genet. 2000 Jul;263(6):1022-30
references: 10954088
references: Plant Cell. 2012 Jan;24(1):123-36
references: 22267485
references: Planta. 2006 Mar;223(4):779-84
references: 16231155
references: Proc Natl Acad Sci U S A. 2012 Mar 27;109(13):5104-9
references: 22411807
references: J Biol Chem. 2002 Feb 15;277(7):5570-4
references: 11734554
references: Curr Genet. 2001 Oct;40(3):209-13
references: 11727997
references: Plant Cell. 2009 Nov;21(11):3686-99
references: 19934379
references: Mol Gen Genet. 1993 Aug;240(2):238-44
references: 8355656
references: Gene. 1994 Sep 2;146(2):177-82
references: 8076816
references: Curr Genet. 2004 May;45(5):331-7
references: 14986064
references: Nature. 2005 Jan 20;433(7023):326-30
references: 15662426
references: Science. 2012 Feb 17;335(6070):848-51
references: 22223739
references: RNA. 2002 Apr;8(4):497-511
references: 11991643
references: EMBO J. 1996 Jun 3;15(11):2802-9
references: 8654377
references: EMBO J. 1999 Jan 4;18(1):249-57
references: 9878067
references: Cell Mol Life Sci. 2006 Mar;63(6):698-708
references: 16465445
references: Plant J. 2005 Sep;43(5):708-15
references: 16115067
references: Proc Natl Acad Sci U S A. 1996 Aug 6;93(16):8766-70
references: 8710946
references: Nucleic Acids Res. 2007;35(17):e114
references: 17726051
references: Nature. 1991 Sep 12;353(6340):178-80
references: 1653905
references: Proc Natl Acad Sci U S A. 2009 Apr 7;106(14):6002-7
references: 19297624
references: Mol Gen Genet. 2000 Nov;264(4):419-24
references: 11129045
references: Nucleic Acids Res. 2003 May 15;31(10):2586-94
references: 12736308
references: Plant J. 1999 Jun;18(5):477-89
references: 10417698
references: Plant Cell. 1992 May;4(5):609-16
references: 1498612
references: J Mol Evol. 2006 Aug;63(2):194-207
references: 16830097
references: Curr Genet. 1997 Jul;32(1):19-23
references: 9309165
references: Plant Mol Biol. 1996 Oct;32(1-2):315-26
references: 8980485
references: Plant J. 2002 Jul;31(2):171-88
references: 12121447