title: Effects of sevoflurane on pulmonary cytosolic phospholipase A₂ and clara cell secretory protein expressions in rabbits with one-lung ventilation-induced lung injury.
abstract: OBJECTIVE
  To investigate the effects of sevoflurane on cytosolic phospholipase A₂ (C-PLA₂) and clara cell secretory protein (CCSP) in lung tissues of rabbits with one-lung ventilation (OLV)-induced lung injuries.
  
  
  METHODS
  Thirty-six healthy Japanese white rabbits were randomized into sham-operated group, OLV group, and OLV plus sevoflurane group subdivided into 4 subgroups with sevoflurane concentrations of 1%, 2%, 3% and 4%. CCSP and C-PLA₂ mRNA and protein expressions in rabbit lung tissues were detected by Western blotting and real-time PCR, and the content of arachidonic acid (AA) was measured using ELISA. The severities of the lung injury were evaluated according to lung wet/dry weight (W/D) ratio and histological scores.
  
  
  RESULTS
  In the OLV group and OLV+ sevoflurane groups, pulmonary CCSP expressions were significantly lower, while C-PLA₂ expression, lung W/D ratios and lung histological scores were significantly higher than those in the sham-operated group (P<0.05). Compared with OLV group, the OLV+sevoflurane groups showed significantly increased expressions of CCSP and reduced C-PLA₂ expression, lung W/D ratios and histological scores (P<0.05). In the 4 OLV+sevoflurane groups, CCSP expressions underwent no significant changes as sevoflurane concentration increased, but C-PLA₂ expressions, lung W/D ratios and histological scores all decreased gradually as the concentrations of sevoflurane increased (P<0.05).
  
  
  CONCLUSIONS
  OLV can result in down-regulated CCSP expressions and up-regulated C-PLA₂ expressions in rabbit lung tissues. Sevoflurane can protect against OLV-induced acute lung injury possibly by inhibiting C-PLA₂ expression via up-regulation of CCSP expressions or through other mechanisms resulting in down-regulated expression of C-PLA₂.
authors: School of Pharmacy and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming 650031, China. liur414324@hotmail.com
authors: Rui
authors: R
authors: Liu
authors: Yong
authors: Y
authors: Yang
authors: Yanhua
authors: Y
authors: Li
authors: Jiang
authors: J
authors: Li
authors: Qingjie
authors: Q
authors: Ma
authors: Yanhua
authors: Y
authors: Zhao
authors: Dianhua
authors: D
authors: Wang
mesh_terms: D000818:Animals; D005260:Female; D008168:Lung / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008738:Methyl Ethers / Q000494:pharmacology*; D061810:One-Lung Ventilation / Q000009:adverse effects; D054467:Phospholipases A2 / Q000378:metabolism*; D011817:Rabbits; D000077149:Sevoflurane; D014598:Uteroglobin / Q000378:metabolism*; D055397:Ventilator-Induced Lung Injury / Q000378:metabolism*
chemical_list: D008738:Methyl Ethers; D000077149:Sevoflurane; D014598:Uteroglobin; D054467:Phospholipases A2