title: Deletion of cavin genes reveals tissue-specific mechanisms for morphogenesis of endothelial caveolae.
abstract: Caveolae are abundant in endothelial cells and are thought to have important roles in endothelial cell biology. The cavin proteins are key components of caveolae, and are expressed at varied amounts in different tissues. Here we use knockout mice to determine the roles of cavins 2 and 3 in caveolar morphogenesis in vivo. Deletion of cavin 2 causes loss of endothelial caveolae in lung and adipose tissue, but has no effect on the abundance of endothelial caveolae in heart and other tissues. Changes in the morphology of endothelium in cavin 2 null mice correlate with changes in caveolar abundance. Cavin 3 is not required for making caveolae in the tissues examined. Cavin 2 determines the size of cavin complexes, and acts to shape caveolae. Cavin 1, however, is essential for normal oligomerization of caveolin 1. Our data reveal that endothelial caveolae are heterogeneous, and identify cavin 2 as a determinant of this heterogeneity.
authors: MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK.
authors: Carsten Gram
authors: CG
authors: Hansen
authors: Elena
authors: E
authors: Shvets
authors: Gillian
authors: G
authors: Howard
authors: Kirsi
authors: K
authors: Riento
authors: Benjamin James
authors: BJ
authors: Nichols
mesh_terms: D000818:Animals; D021941:Caveolae / Q000378:metabolism*; D051242:Caveolin 1 / Q000737:chemistry / Q000378:metabolism; D048430:Cell Shape; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D004727:Endothelium / Q000254:growth & development* / Q000378:metabolism* / Q000648:ultrastructure; D017353:Gene Deletion; D008168:Lung / Q000166:cytology / Q000378:metabolism / Q000648:ultrastructure; D008565:Membrane Proteins / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008970:Molecular Weight; D009024:Morphogenesis; D046912:Multiprotein Complexes / Q000378:metabolism; D009206:Myocardium / Q000378:metabolism / Q000648:ultrastructure; D009928:Organ Specificity; D020836:Protein Structure, Quaternary; D016601:RNA-Binding Proteins
chemical_list: D051242:Caveolin 1; C112745:Cavin1 protein, mouse; D008565:Membrane Proteins; D046912:Multiprotein Complexes; D016601:RNA-Binding Proteins
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