title: Mechanism of T-cell mediated protection in newborn mice against a Chlamydia infection.
abstract: To determine the immune components needed for protection of newborn mice against Chlamydia muridarum, animals born to Chlamydia-immunized and to sham-immunized dams were infected intranasally with C. muridarum at 2 post-natal days. T-cells isolated from immunized or sham-immunized adult mice were adoptively transferred to newborn mice at the time of infection. Also, to establish what cytokines are involved in protection, IFN-γ, TNF-α, IL-10, and IL-12 were passively transferred to newborn mice. To assess the Chlamydia burden in the lungs mice were euthanized at 12 post-natal days. When T-cells from immunized adult mice were transferred, mice born to and fed by immunized dams were significantly protected as evidenced by the reduced number of Chlamydia isolated from the lungs compared to mice born to and fed by sham-immunized dams. Transfer of IFN-γ and TNF-α also significantly reduced the number of Chlamydia in the lungs of mice born to immunized dams. Transfer of IL-10 or IL-12 did not result in a significant reduction of Chlamydia. In vitro T-cell proliferation data suggest that neonatal antigen presenting cells can present Chlamydia antigens to adult T-cells. In conclusion, maternal antibodies and Chlamydia specific T-cells or Th1 cytokines are required for protection of neonates against this pathogen.
authors: Department of Pathology and Laboratory Medicine, Medical Science Building 1, Room # D440, University of California, Irvine, Irvine, CA 92697-4800, USA. spal@uci.edu
authors: Sukumar
authors: S
authors: Pal
authors: Luis M
authors: LM
authors: de la Maza
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D000831:Animals, Newborn; D000907:Antibodies, Bacterial / Q000276:immunology; D058491:Bacterial Load; D049109:Cell Proliferation; D002478:Cells, Cultured; D002690:Chlamydia Infections / Q000276:immunology*; D023502:Chlamydia muridarum / Q000276:immunology*; D016207:Cytokines / Q000008:administration & dosage / Q000276:immunology; D005260:Female; D007112:Immunity, Maternally-Acquired; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008168:Lung / Q000382:microbiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D016207:Cytokines
keywords: Chlamydia muridarum; Maternal antibodies; Neonatal immunity; Neonatal mouse model; Passive immunity
references: J Immunol. 1982 Jul;129(1):370-6
references: 6282968
references: J Exp Med. 2008 Sep 29;205(10):2269-80
references: 18762566
references: N Engl J Med. 2001 Nov 1;345(18):1331-5
references: 11794153
references: J Immunol. 2008 Mar 1;180(5):3375-82
references: 18292563
references: Infect Immun. 2006 Apr;74(4):1999-2006
references: 16552028
references: Ann N Y Acad Sci. 1988;549:24-30
references: 3228254
references: Infect Immun. 2010 Oct;78(10):4374-83
references: 20660610
references: Reg Immunol. 1993 Nov-Dec;5(6):317-24
references: 8068534
references: Infect Immun. 1994 Aug;62(8):3354-62
references: 8039906
references: Immunology. 2002 Feb;105(2):213-21
references: 11872097
references: J Immunol. 1982 Mar;128(3):1107-11
references: 6173420
references: Infect Immun. 1989 Jan;57(1):152-7
references: 2491833
references: Trends Immunol. 2009 Dec;30(12):585-91
references: 19846341
references: Infect Immun. 1990 Jun;58(6):1572-6
references: 2341167
references: Nat Rev Immunol. 2007 May;7(5):379-90
references: 17457344
references: J Reprod Immunol. 2010 Nov;86(2):151-7
references: 20554327
references: Infect Immun. 1981 Mar;31(3):1161-76
references: 7228399
references: BMC Infect Dis. 2003 Apr 04;3:2
references: 12697048
references: J Immunol. 2008 Sep 1;181(5):3486-94
references: 18714021
references: Infect Immun. 2002 Jun;70(6):2741-51
references: 12010958
references: J Immunol. 1998 Sep 15;161(6):3143-9
references: 9743382
references: Clin Diagn Lab Immunol. 1997 Sep;4(5):607-10
references: 9302214
references: J Autoimmun. 1991 Apr;4(2):249-62
references: 1909136
references: J Interferon Cytokine Res. 2010 Jun;30(6):407-15
references: 20187773
references: Science. 1996 Mar 22;271(5256):1723-6
references: 8596932
references: J Immunol. 1999 Jan 15;162(2):1010-7
references: 9916727
references: Infect Immun. 1996 Jul;64(7):2850-2
references: 8698523
references: Proc Natl Acad Sci U S A. 1993 May 1;90(9):3998-4002
references: 8387206
references: Arch Dis Child. 1986 Jun;61(6):565-8
references: 3729525
references: Am J Epidemiol. 1990 Nov;132(5):946-52
references: 2239909
references: Science. 1942 Jan 9;95(2454):49-50
references: 17773453
references: Infect Immun. 2000 Dec;68(12):6979-87
references: 11083822
references: Infect Immun. 2002 Sep;70(9):5132-9
references: 12183563
references: J Immunol Methods. 1995 Mar 27;180(2):147-57
references: 7714330
references: Annu Rev Microbiol. 1980;34:285-309
references: 7002026
references: Semin Pediatr Infect Dis. 2005 Oct;16(4):235-44
references: 16210104
references: Science. 1996 Mar 22;271(5256):1728-30
references: 8596934
references: Proc Natl Acad Sci U S A. 1978 Oct;75(10):5127-31
references: 154104
references: Proc Natl Acad Sci U S A. 2005 Jul 26;102(30):10658-63
references: 16020528
references: Infect Immun. 1997 Jun;65(6):1993-9
references: 9169723
references: MMWR Morb Mortal Wkly Rep. 2009 Apr 17;58(14):362-5
references: 19373196
references: J Immunol. 1982 Jan;128(1):337-42
references: 6459373
references: Infect Immun. 2002 Sep;70(9):4812-7
references: 12183524
references: Vaccine. 1995 Jan;13(1):119-27
references: 7762268
references: J Trop Pediatr. 1996 Aug;42(4):233-6
references: 8816036