title: New alternative promoter in regulation of the Oct-1 human gene transcription.
abstract: For the first time, the presence of a new alternative promoter in the gene of the oct-1 transcription factor from which a previously unknown mRNA isoform Oct-1X, with 5'-terminus different from the previously described isoforms, was demonstrated. The nucleotide sequence of the Oct-1X cDNA was determined and the presence of a long open reading frame which starts with the first ATG codon of the second exon was demonstrated. A protein shortened on its N-terminus is assumed to be the product of a new mRNA isoform. Expression of the new isoform was studied in various human cell lines; the results indicate the ubiquitous nature of the Oct-1X expression. Therefore, in the work, the third alternative promoter of human oct-1 gene (earlier, a ubiquitous promoter U and an inducible tissue-specific promoter L were described) contributing to the fine regulation of mRNA isoform synthesis and formation of structural and functional diversity of Oct-1 protein isoforms in cells was revealed.
authors: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
authors: T N
authors: TN
authors: Portseva
authors: I D
authors: ID
authors: Krylova
authors: S G
authors: SG
authors: Georgieva
authors: A G
authors: AG
authors: Stepchenko
authors: E V
authors: EV
authors: Pankratova
mesh_terms: D002460:Cell Line; D005091:Exons / Q000235:genetics; D006801:Humans; D050811:Octamer Transcription Factor-1 / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D020033:Protein Isoforms / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000235:genetics*
chemical_list: D050811:Octamer Transcription Factor-1; D020033:Protein Isoforms; D012333:RNA, Messenger
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