title: Decreased PLUNC expression in nasal polyps is associated with multibacterial colonization in chronic rhinosinusitis patients.
abstract: PLUNC (palate, lung, and nasal epithelium clone) is an epithelium-secreted protein that plays a crucial role in the host's defense against bacterial infection. The function of PLUNC in the sinus remains poorly understood. To examine whether the expression levels of PLUNC could serve as a predictive outcome biomarker for patients with CRSwNP and bacterial colonization, we investigated the association of PLUNC expression levels with bacterial colonization in the sinuses. A total of 174 patients who underwent sinus surgery for chronic rhinosinusitis with nasal polyps (CRSwNP) were enrolled in this study. The tissue samples obtained from patients were examined using preoperative sinus computed tomography (CT) scans, postoperative bacterial cultures, and nasal polyp examinations. PLUNC mRNA and protein expression were quantified using RT-PCR and immunohistochemistry. We identified that decreased PLUNC expression is associated with multibacterial colonization (P = 0.0001), specifically those mediated by Staphyloccocus aureus (P = 0.037) and Pseudomonas aeruginosa (P = 0.002). The patients who required repeated sinus surgeries for recurrent or persistent sinusitis also presented much lower PLUNC expression than those who did not require repeated sinus surgery (P = 0.001). However, gender, age, and CT scores were not associated with PLUNC expression. These results suggest that reduced PLUNC expression is associated with bacterial colonization as well as treatment outcome in CRSwNP patients. Investigation of the association between PLUNC expressions and chronic rhinosinusitis may lead to the development of a novel biomarker for treatment outcome in CRSwNP patients.
authors: Department of Otolaryngology-Head and Neck Surgery, China Medical University Hospital, Taichung, Taiwan.
authors: Yung-An
authors: YA
authors: Tsou
authors: Ming-Te
authors: MT
authors: Peng
authors: Yi-Fang
authors: YF
authors: Wu
authors: Chih-Ho
authors: CH
authors: Lai
authors: Chia-Der
authors: CD
authors: Lin
authors: Chih-Jaan
authors: CJ
authors: Tai
authors: Ming-Hsui
authors: MH
authors: Tsai
authors: Chuan-Mu
authors: CM
authors: Chen
authors: Hui-Chen
authors: HC
authors: Chen
mesh_terms: D000328:Adult; D002908:Chronic Disease; D060085:Coinfection; D005260:Female; D006023:Glycoproteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009297:Nasal Mucosa / Q000378:metabolism; D009298:Nasal Polyps / Q000150:complications / Q000235:genetics* / Q000378:metabolism; D010750:Phosphoproteins / Q000235:genetics* / Q000378:metabolism; D011552:Pseudomonas Infections / Q000150:complications / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification; D012333:RNA, Messenger / Q000032:analysis*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012220:Rhinitis / Q000150:complications / Q000382:microbiology*; D012852:Sinusitis / Q000150:complications / Q000382:microbiology*; D013203:Staphylococcal Infections / Q000150:complications / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000302:isolation & purification; D055815:Young Adult
chemical_list: C485599:BPIFA1 protein, human; D006023:Glycoproteins; D010750:Phosphoproteins; D012333:RNA, Messenger
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