title: Characterization and modeling of transcriptional cross-regulation in Acinetobacter baylyi ADP1.
abstract: Synthetic biology involves reprogramming and engineering of regulatory genes in innovative ways for the implementation of novel tasks. Transcriptional gene regulation systems induced by small molecules in prokaryotes provide a rich source for logic gates. Cross-regulation, whereby a promoter is activated by different molecules or different promoters are activated by one molecule, can be used to design an OR-gate and achieve cross-talk between gene networks in cells. Acinetobacter baylyi ADP1 is naturally transformable, readily editing its chromosomal DNA, which makes it a convenient chassis for synthetic biology. The catabolic genes for salicylate, benzoate, and catechol metabolism are located within a supraoperonic cluster (-sal-are-ben-cat-) in the chromosome of A. baylyi ADP1, which are separately regulated by LysR-type transcriptional regulators (LTTRs). ADP1-based biosensors were constructed in which salA, benA, and catB were fused with a reporter gene cassette luxCDABE under the separate control of SalR, BenM, and CatM regulators. Salicylate, benzoate, catechol, and associated metabolites were found to mediate cross-regulation among sal, ben, and cat operons. A new mathematical model was developed by considering regulator-inducer binding and promoter activation as two separate steps. This model fits the experimental data well and is shown to predict cross-regulation performance.
authors: Kroto Research Institute, University of Sheffield, Broad Lane, Sheffield S3 7HQ, UK.
authors: Dayi
authors: D
authors: Zhang
authors: Yun
authors: Y
authors: Zhao
authors: Yi
authors: Y
authors: He
authors: Yun
authors: Y
authors: Wang
authors: Yiyu
authors: Y
authors: Zhao
authors: Yi
authors: Y
authors: Zheng
authors: Xia
authors: X
authors: Wei
authors: Litong
authors: L
authors: Zhang
authors: Yuzhen
authors: Y
authors: Li
authors: Tao
authors: T
authors: Jin
authors: Lin
authors: L
authors: Wu
authors: Hui
authors: H
authors: Wang
authors: Paul A
authors: PA
authors: Davison
authors: Junguang
authors: J
authors: Xu
authors: Wei E
authors: WE
authors: Huang
mesh_terms: D000150:Acinetobacter / Q000235:genetics* / Q000378:metabolism*; D015374:Biosensing Techniques; D015964:Gene Expression Regulation, Bacterial; D053263:Gene Regulatory Networks; D005798:Genes, Bacterial; D005818:Genetic Engineering; D053858:Metabolic Networks and Pathways / Q000235:genetics; D008957:Models, Genetic; D058615:Synthetic Biology; D014158:Transcription, Genetic