title: A fluoroimmunoassay based on quantum dot-streptavidin conjugate for the detection of chlorpyrifos.
abstract: A rapid and sensitive competitive fluorescence-linked immunosorbent assay (cFLISA) based on quantum dot-streptavidin conjugate (QDs-SA) was developed for the detection of chlorpyrifos in drinking water. The QDs-SA conjugate, which consists of 3-mercaptopropyl acid-stabilized CdTe nanoparticle QDs and streptavidin (SA) made through the active ester method, was employed to improve the sensitivity of QDs-SA-cFLISA. The 50% inhibition concentration (IC50) and the limit of detection (LOD) were 28.5 and 3.8 ng mL(-1), respectively. QDs-SA-cFLISA increased sensitivity 5.5-fold and reduced detection time by 1 h compared with conventional enzyme-linked immunosorbent assay (ELISA). With chlorpyrifos concentrations of 100, 50, and 20 ng mL(-1), recoveries ranged from 85.9% to 105.3% with coefficients of variation ranging from 6.3% to 13.5%. This study demonstrated that QDs-SA-cFLISA was more rapid and sensitive than conventional ELISA. Therefore, it can be used as a novel screening method for the detection of pesticide residues.
authors: College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors: Yiping
authors: Y
authors: Chen
authors: He Ling
authors: HL
authors: Ren
authors: Nan
authors: N
authors: Liu
authors: Na
authors: N
authors: Sai
authors: Xiaoyu
authors: X
authors: Liu
authors: Zhen
authors: Z
authors: Liu
authors: Zhixian
authors: Z
authors: Gao
authors: Bao an
authors: Ba
authors: Ning
mesh_terms: D004390:Chlorpyrifos / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D015200:Fluoroimmunoassay / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D007306:Insecticides / Q000032:analysis*; D057230:Limit of Detection; D045663:Quantum Dots; D019809:Streptavidin / Q000737:chemistry*
chemical_list: D007306:Insecticides; D019809:Streptavidin; D004390:Chlorpyrifos