title: Multiple pathways suppress non-allelic homologous recombination during meiosis in Saccharomyces cerevisiae.
abstract: Recombination during meiosis in the form of crossover events promotes the segregation of homologous chromosomes by providing the only physical linkage between these chromosomes. Recombination occurs not only between allelic sites but also between non-allelic (ectopic) sites. Ectopic recombination is often suppressed to prevent non-productive linkages. In this study, we examined the effects of various mutations in genes involved in meiotic recombination on ectopic recombination during meiosis. RAD24, a DNA damage checkpoint clamp-loader gene, suppressed ectopic recombination in wild type in the same pathway as RAD51. In the absence of RAD24, a meiosis-specific recA homolog, DMC1, suppressed the recombination. Homology search and strand exchange in ectopic recombination occurred when either the RAD51 or the DMC1 recA homolog was absent, but was promoted by RAD52. Unexpectedly, the zip1 mutant, which is defective in chromosome synapsis, showed a decrease, rather than an increase, in ectopic recombination. Our results provide evidence for two types of ectopic recombination: one that occurs in wild-type cells and a second that occurs predominantly when the checkpoint pathway is inactivated.
authors: Division of Integrated Protein Functions, Institute for Protein Research, Osaka University, Suita, Osaka, Japan. mikis@protein.osaka-u.ac.jp
authors: Miki
authors: M
authors: Shinohara
authors: Akira
authors: A
authors: Shinohara
mesh_terms: D000483:Alleles; D018797:Cell Cycle Proteins / Q000378:metabolism; D053903:DNA Breaks, Double-Stranded; D004265:DNA Helicases / Q000378:metabolism; D026942:DNA Topoisomerases / Q000378:metabolism; D004843:Epistasis, Genetic; D059765:Homologous Recombination; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D008540:Meiosis; D008954:Models, Biological; D009687:Nuclear Proteins / Q000235:genetics; D051135:Rad51 Recombinase / Q000378:metabolism; D051721:Rad52 DNA Repair and Recombination Protein; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D015398:Signal Transduction
chemical_list: D018797:Cell Cycle Proteins; D047908:Intracellular Signaling Peptides and Proteins; D009687:Nuclear Proteins; C088385:RAD24 protein, S cerevisiae; C497197:RAD52 protein, S cerevisiae; D051721:Rad52 DNA Repair and Recombination Protein; D029701:Saccharomyces cerevisiae Proteins; C090118:Zip1 protein, S cerevisiae; D051135:Rad51 Recombinase; D004265:DNA Helicases; D026942:DNA Topoisomerases; C541336:RDH54 protein, S cerevisiae
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