title: Novel insight into the distribution of L-cells in the rat intestinal tract.
abstract: BACKGROUND
  Gut secreted incretin hormones and gastric bypass surgery currently provides some of the most successful treatments for diabetes and obesity respectively. However, despite the evident importance of the gut endocrine system no information exists on the total number and distribution of different types of endocrine cells in the gut. Here we have used the established preclinical Zucker Diabetic Fatty (ZDF) rat model which displays elevated levels of GLP-1 to assess L-cell distribution and L-cell dynamics in the full rostro-caudal extension of the rat intestinal tract.
  
  
  METHODS
  Using mathematically unbiased stereology we provide total and regional estimates of gut volume, gut surface area and the total number of L-cells throughout the intestinal tract in obese ZDF rats and lean controls.
  
  
  RESULTS
  The total number of L-cells in the lean and obese ZDF gut is estimated to 4.8 and 10.9 million, respectively, coupled with a corresponding near doubling in total gut volume and total surface area. L-cell numbers were found to be distributed rather evenly throughout the jejunum, ileum and colon.
  
  
  CONCLUSIONS
  The present study provides the first stereological report of total L-cell number and L-cell distribution throughout the rat intestinal tract. In contrast to the currently held view, the majority of L-cells are actually located proximal to the traditionally defined ileum and colon.
authors: Gubra Aps Agern Alle 1, 2970 Hørsholm ; Department of Human Nutrition, University of Copenhagen Frederiksberg,Denmark.
authors: Carl Frederik
authors: CF
authors: Hansen
authors: Niels
authors: N
authors: Vrang
authors: Per Torp
authors: PT
authors: Sangild
authors: Jacob
authors: J
authors: Jelsing
keywords: GLP-1; L-cell; ZDF; diabetes; stereology
references: Gastroenterology. 2010 Jun;138(7):2437-46, 2446.e1
references: 20226188
references: Ann Surg. 2006 Jan;243(1):108-14
references: 16371744
references: Brain Res. 2008 Jan 2;1187:116-24
references: 18022140
references: Cell Metab. 2006 Mar;3(3):153-65
references: 16517403
references: Cereb Cortex. 2011 Nov;21(11):2519-24
references: 21427166
references: Gut. 1971 Apr;12(4):311-8
references: 4930155
references: J Vet Med Sci. 1999 Jul;61(7):761-7
references: 10458098
references: Dig Dis Sci. 2002 Apr;47(4):868-78
references: 11991623
references: Endocrinology. 1993 Jul;133(1):233-40
references: 8319572
references: Cell Tissue Res. 1992 Dec;270(3):577-85
references: 1336714
references: BMC Med Genomics. 2008 May 06;1:14
references: 18457598
references: J Microsc. 1984 May;134(Pt 2):127-36
references: 6737468
references: Int J Obes (Lond). 2008 Dec;32 Suppl 7:S93-7
references: 19136998
references: Anat Embryol (Berl). 2002 May;205(2):153-7
references: 12021917
references: Anat Rec (Hoboken). 2011 Sep;294(9):1557-65
references: 21809459
references: J Microsc. 1999 Mar;193(Pt 3):199-211
references: 10348656
references: Brain Res. 2007 May 29;1149:118-26
references: 17433266
references: Physiol Rev. 1996 Oct;76(4):1109-61
references: 8874496
references: J Anat. 1985 Aug;141:1-17
references: 4077708
references: J Histochem Cytochem. 1985 Oct;33(10):1080-6
references: 3900195
references: Diabetes. 2003 Feb;52(2):252-9
references: 12540594
references: Z Gastroenterol. 1996 Mar;34(3):159-66
references: 8650968
references: Diabetes Obes Metab. 2012 Apr;14(4):291-8
references: 21951387
references: APMIS. 1999 Aug;107(8):791-9
references: 10515130
references: Dig Dis Sci. 2011 Sep;56(9):2566-74
references: 21374061
references: J Microsc. 1988 Apr;150(Pt 1):1-20
references: 3043005
references: Endocrinology. 2012 Dec;153(12):5782-95
references: 23064014
references: J Clin Endocrinol Metab. 2005 Jan;90(1):359-65
references: 15483088
references: Ann Surg. 1995 Sep;222(3):339-50; discussion 350-2
references: 7677463
references: Clin Endocrinol (Oxf). 2008 Aug;69(2):173-9
references: 18167136
references: J Clin Endocrinol Metab. 1997 Mar;82(3):786-90
references: 9062483
references: Gastroenterology. 2010 May;138(5):1845-53
references: 19931268
references: Regul Pept. 2003 Jul 15;114(2-3):189-96
references: 12832109
references: Digestion. 1995;56(2):117-26
references: 7750665
references: Proc Natl Acad Sci U S A. 1996 Jul 23;93(15):7911-6
references: 8755576
references: Endocrinology. 2012 Jul;153(7):3076-88
references: 22569789
references: Physiol Rev. 2007 Oct;87(4):1409-39
references: 17928588
references: J Endocrinol. 1993 Jul;138(1):159-66
references: 7852887
references: Eur J Clin Invest. 1992 Apr;22(4):283-91
references: 1499644
references: Ann Surg. 2010 Jun;251(6):1049-55
references: 20485144
references: Metabolism. 1997 Mar;46(3):261-7
references: 9054467
references: Gut. 1979 Aug;20(8):653-9
references: 114457
references: J Microsc. 1984 Sep;135(Pt 3):337-46
references: 6492144
references: Gut. 2010 Feb;59(2):186-96
references: 19074180
references: Endocrinology. 1986 Oct;119(4):1467-75
references: 3530719
references: J Histochem Cytochem. 1991 Mar;39(3):341-9
references: 1899685
references: Am J Med. 2009 Mar;122(3):248-256.e5
references: 19272486
references: Br J Nutr. 2007 Jul;98(1):32-7
references: 17367575
references: J Anat. 2001 Dec;199(Pt 6):725-34
references: 11787826
references: Endocrinology. 2012 Jul;153(7):3054-65
references: 22685263
references: Lab Anim. 1999 Oct;33(4):393-400
references: 10778790
references: Am J Physiol. 1997 Jun;272(6 Pt 1):E1050-8
references: 9227451
references: Endocrinology. 1999 Apr;140(4):1687-94
references: 10098504
references: Proc Nutr Soc. 2012 Nov;71(4):456-62
references: 22906726
references: Am J Physiol Gastrointest Liver Physiol. 2009 Nov;297(5):G950-7
references: 20501442
references: Gastroenterology. 1995 Apr;108(4):967-74
references: 7698612
references: J Microsc. 1987 Sep;147(Pt 3):229-63
references: 3430576
references: Trends Pharmacol Sci. 2011 Jan;32(1):8-15
references: 21130506
references: Histochemistry. 1987;88(1):37-40
references: 3325477
references: Diabetes. 2006 Dec;55(12):3387-93
references: 17130484
references: J Clin Endocrinol Metab. 1983 Sep;57(3):488-95
references: 6874888
references: Virchows Arch B Cell Pathol Incl Mol Pathol. 1988;56(3):191-9
references: 2906190
references: J Gastrointest Surg. 1998 Mar-Apr;2(2):146-50
references: 9834410
references: APMIS. 1988 Oct;96(10):857-81
references: 3056461
references: Diabetologia. 2011 Jan;54(1):10-8
references: 20871975
references: Ann Surg. 2003 Oct;238(4):467-84; discussion 84-5
references: 14530719