title: Diagnosis of nitrosative stress by quantitative EPR-spectroscopy of epidermal cells.
abstract: Quantitative assay of nitric oxide (NO) and iron (Fe) was carried out by the electron paramagnetic resonance (EPR) method in epidermal derivative (hair) of 45 liquidators of the Chernobyl accident, 30 employees of radiological institutions having contact with radiation, and 50 students of Vladimir State University, most of which were diagnosed with iron deficiency, namely iron deficiency anemia (4 persons) and latent iron deficiency (34 persons). Measurements were performed on a Radiopan EPR-spectrometer. It was found that the method of quantitative EPR-spectroscopy using diethyldithiocarbamate as a trap in vitro could be successfully used to measure NO-radical activity in such a biological substrate as hair. It was found that the intensity of NO-radical signal in the spectrogram depends on the Fe level (not only in the analyzed substrate, but also in the whole organism).
authors: Vladimir State University, Russia. vip-val@yandex.ru
authors: V I
authors: VI
authors: Petukhov
authors: L K
authors: LK
authors: Baumane
authors: E D
authors: ED
authors: Reste
authors: T Y
authors: TY
authors: Zvagule
authors: M A
authors: MA
authors: Romanova
authors: N I
authors: NI
authors: Shushkevich
authors: L T
authors: LT
authors: Sushkova
authors: S V
authors: SV
authors: Skavronsky
authors: A N
authors: AN
authors: Shchukov
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D018798:Anemia, Iron-Deficiency / Q000378:metabolism*; D016022:Case-Control Studies; D047852:Chernobyl Nuclear Accident; D004578:Electron Spin Resonance Spectroscopy; D004817:Epidermis / Q000473:pathology; D005260:Female; D005293:Ferritins / Q000097:blood; D006197:Hair / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000378:metabolism*; D016273:Occupational Exposure; D011850:Radioactive Waste; D013312:Stress, Physiological / Q000528:radiation effects*; D014455:Ukraine; D055815:Young Adult
chemical_list: D011850:Radioactive Waste; D009569:Nitric Oxide; D005293:Ferritins