title: Serum microRNAs profile from genome-wide serves as a fingerprint for diagnosis of acute myocardial infarction and angina pectoris.
abstract: BACKGROUND
  In order to identify miRNAs expression profiling from genome-wide screen for diagnosis of acute myocardial infarction (AMI) and angina pectoris (AP), we investigated the altered profile of serum microRNAs in AMI and AP patients at a relative early stage.
  
  
  METHODS
  Serum samples were taken from 117 AMI patients, 182 AP patients and 100 age-and gender-matched controls. An initial screening of miRNAs expression was performed by Solexa sequencing. Differential expression was validated using RT-qPCR in individuals samples, the samples were arranged in a two-phase selection and validation.
  
  
  RESULTS
  The Solexa sequencing results demonstrated marked upregulation of serum miRNAs in AMI patients compared with controls. RT-qPCR analysis identified a profile of six serum miRNAs (miR-1, miR-134, miR-186, miR-208, miR-223 and miR-499) as AMI biomarkers. MiR-208 and miR-499 were elevated higher in AP cases than in AMI cases. The ROC curves indicated a panel of six miRNAs has a great potential to offer sensitive and specific diagnostic tests for AMI. More especially, the panel of six miRNAs presents significantly differences between the AMI and AP cases.
  
  
  CONCLUSIONS
  The six-miRNAs signature identified from genome-wide serum miRNA expression profiling may serves as a fingerprint for AMI and AP diagnosis.
authors: Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, and State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 22 Hankou Rd, Nanjing 210093, China.
authors: Chunjian
authors: C
authors: Li
authors: Zhijuan
authors: Z
authors: Fang
authors: Ting
authors: T
authors: Jiang
authors: Qiu
authors: Q
authors: Zhang
authors: Chao
authors: C
authors: Liu
authors: Chenyu
authors: C
authors: Zhang
authors: Yang
authors: Y
authors: Xiang
mesh_terms: D000368:Aged; D000787:Angina Pectoris / Q000175:diagnosis* / Q000235:genetics; D019540:Area Under Curve; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D015894:Genome, Human; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D009203:Myocardial Infarction / Q000175:diagnosis* / Q000235:genetics; D012372:ROC Curve; D017423:Sequence Analysis, RNA
chemical_list: D015415:Biomarkers; D035683:MicroRNAs
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