title: Induction of albumin expression in HepG2 cells using immobilized simplified recombinant fibronectin protein.
abstract: Optimization of the extracellular environment is very important for hepatocyte function in vitro. We expressed new chimeric proteins of the collagen-binding domain (CBD) with cell attachment site (CAS) of fibronectin to enhance hepatocyte function, and the CBD-CAS proteins were immobilized on collagen-coated plates. We hypothesized that the high density of CAS would increase activity of the integrin-dependent intracellular signaling pathway, thus inducing hepatocyte function. Expression of albumin in the human hepatocyte cell line HepG2 was assessed on CBD-CAS-immobilized dishes. The results indicated that the CBD-CAS-immobilized plates induced albumin expression. Immobilized CBD-CAS induced activation of focal adhesion kinase and integrin-ligand clustering on the cell membrane. These results suggest that immobilized CBD-CAS improves the function of HepG2 cells. This system could therefore be applied to drug metabolism assay in the development of new drugs.
authors: Cell-Materials Interaction Group, Biomaterials Unit, Nano-Life Field, International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba, Ibaraki, 305-0044, Japan.
authors: Yuuki
authors: Y
authors: Nishida
authors: Akiyoshi
authors: A
authors: Taniguchi
mesh_terms: D000418:Albumins / Q000096:biosynthesis* / Q000378:metabolism; D002465:Cell Movement / Q000235:genetics; D003094:Collagen / Q000737:chemistry / Q000235:genetics; D005353:Fibronectins / Q000737:chemistry / Q000378:metabolism*; D051417:Focal Adhesion Kinase 1 / Q000378:metabolism; D005786:Gene Expression Regulation; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000378:metabolism*; D006801:Humans; D010766:Phosphorylation; D017434:Protein Structure, Tertiary / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D000418:Albumins; D005353:Fibronectins; D011993:Recombinant Fusion Proteins; D003094:Collagen; D051417:Focal Adhesion Kinase 1
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