title: C. elegans GATA factors EGL-18 and ELT-6 function downstream of Wnt signaling to maintain the progenitor fate during larval asymmetric divisions of the seam cells.
abstract: The C. elegans seam cells are lateral epithelial cells arrayed in a single line from anterior to posterior that divide in an asymmetric, stem cell-like manner during larval development. These asymmetric divisions are regulated by Wnt signaling; in most divisions, the posterior daughter in which the Wnt pathway is activated maintains the progenitor seam fate, while the anterior daughter in which the Wnt pathway is not activated adopts a differentiated hypodermal fate. Using mRNA tagging and microarray analysis, we identified the functionally redundant GATA factor genes egl-18 and elt-6 as Wnt pathway targets in the larval seam cells. EGL-18 and ELT-6 have previously been shown to be required for initial seam cell specification in the embryo. We show that in larval seam cell asymmetric divisions, EGL-18 is expressed strongly in the posterior seam-fated daughter. egl-18 and elt-6 are necessary for larval seam cell specification, and for hypodermal to seam cell fate transformations induced by ectopic Wnt pathway overactivation. The TCF homolog POP-1 binds a site in the egl-18 promoter in vitro, and this site is necessary for robust seam cell expression in vivo. Finally, larval overexpression of EGL-18 is sufficient to drive expression of a seam marker in other hypodermal cells in wild-type animals, and in anterior hypodermal-fated daughters in a Wnt pathway-sensitized background. These data suggest that two GATA factors that are required for seam cell specification in the embryo independently of Wnt signaling are reused downstream of Wnt signaling to maintain the progenitor fate during stem cell-like divisions in larval development.
authors: Department of Biological Sciences, University of Maryland Baltimore County, 1000 Hilltop Circle, Baltimore, MD 21250, USA.
authors: Lakshmi
authors: L
authors: Gorrepati
authors: Kenneth W
authors: KW
authors: Thompson
authors: David M
authors: DM
authors: Eisenmann
mesh_terms: D000483:Alleles; D000818:Animals; D017173:Caenorhabditis elegans / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism*; D002454:Cell Differentiation; D019070:Cell Lineage; D050980:GATA Transcription Factors / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D020411:Oligonucleotide Array Sequence Analysis; D011401:Promoter Regions, Genetic; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction; D051153:Wnt Proteins / Q000378:metabolism*
chemical_list: D029742:Caenorhabditis elegans Proteins; D050980:GATA Transcription Factors; D012333:RNA, Messenger; D051153:Wnt Proteins; C434745:egl-18 protein, C elegans; C434746:elt-6 protein, C elegans
references: WormBook. 2006 Jan 09;:1-16
references: 18050480
references: Development. 1994 Sep;120(9):2359-68
references: 7956818
references: Genome Biol. 2007;8(7):R135
references: 17612406
references: Mol Cell Biol. 2001 Apr;21(7):2533-44
references: 11259601
references: Dev Biol. 2005 Sep 15;285(2):510-23
references: 16084508
references: Dev Biol. 2010 Dec 1;348(1):58-66
references: 20849842
references: Dev Biol. 2002 Feb 15;242(2):96-108
references: 11820809
references: Dev Biol. 1977 Mar;56(1):110-56
references: 838129
references: Genes Dev. 2005 Aug 1;19(15):1743-8
references: 16077003
references: Dev Biol. 2004 Aug 15;272(2):536-57
references: 15282167
references: Development. 2002 Mar;129(6):1497-508
references: 11880358
references: Genetics. 1997 May;146(1):185-206
references: 9136010
references: Dev Dyn. 2010 May;239(5):1539-54
references: 20419785
references: Cell. 2011 Sep 16;146(6):942-54
references: 21925317
references: Development. 2005 Feb;132(4):739-49
references: 15659483
references: Genes Dev. 1998 Dec 15;12(24):3809-14
references: 9869634
references: PLoS Genet. 2011 Oct;7(10):e1002308
references: 22022276
references: Cell Cycle. 2010 Dec 1;9(23):4748-65
references: 21127398
references: EMBO J. 2008 Jun 18;27(12):1647-57
references: 18497747
references: Genes Dev. 1997 Jul 1;11(13):1651-61
references: 9224715
references: Dev Biol. 2002 Aug 1;248(1):128-42
references: 12142026
references: Development. 1994 May;120(5):1035-47
references: 8026318
references: Dev Dyn. 2011 Mar;240(3):649-62
references: 21305652
references: J Cell Biochem. 2007 Apr 1;100(5):1119-30
references: 17265434
references: Cell. 2008 Jul 25;134(2):291-303
references: 18662544
references: Development. 2011 Oct;138(20):4375-85
references: 21937595
references: Nucleic Acids Res. 2010 Jan;38(Database issue):D463-7
references: 19910365
references: Dev Biol. 2010 Sep 15;345(2):144-55
references: 20624379
references: Dev Cell. 2009 Jul;17(1):27-34
references: 19619489
references: Development. 2001 Aug;128(15):2867-80
references: 11532911
references: Dev Biol. 2007 Sep 15;309(2):259-72
references: 17706957
references: Dev Biol. 2009 Sep 15;333(2):337-47
references: 19607822
references: Genetics. 2000 Nov;156(3):1097-116
references: 11063687
references: Trends Cell Biol. 2007 Oct;17(10):465-73
references: 17919911
references: Dev Cell. 2006 Jul;11(1):105-15
references: 16824957
references: Nature. 2000 Aug 3;406(6795):527-32
references: 10952315
references: Chromosoma. 2009 Aug;118(4):405-18
references: 19340450
references: Cell. 1998 Jan 23;92(2):229-39
references: 9458047
references: IUBMB Life. 2009 Aug;61(8):800-30
references: 19621348
references: Genes Cells. 2010 Apr 1;15(4):397-407
references: 20298434
references: Genome Res. 2002 Jun;12(6):996-1006
references: 12045153
references: Dev Biol. 2005 Sep 15;285(2):584-92
references: 16112103
references: Nucleic Acids Res. 2001 May 1;29(9):e45
references: 11328886
references: Cell. 2012 Jun 8;149(6):1192-205
references: 22682243
references: Methods Mol Biol. 2008;469:87-102
references: 19109705
references: PLoS Genet. 2011 Mar;7(3):e1002010
references: 21408209
references: Nucleic Acids Res. 2012 Jan;40(Database issue):D735-41
references: 22067452
references: Proc Natl Acad Sci U S A. 2007 Dec 18;104(51):20368-73
references: 18077322
references: Development. 2002 Nov;129(22):5171-80
references: 12399309
references: Semin Cell Dev Biol. 2002 Jun;13(3):233-41
references: 12137732
references: Dev Biol. 2005 Nov 15;287(2):262-73
references: 16226243
references: Dev Dyn. 2010 May;239(5):1477-89
references: 20232378
references: Cold Spring Harb Perspect Biol. 2012 Aug 01;4(8):a007948
references: 22745286
references: Dev Biol. 1998 Jun 15;198(2):286-302
references: 9659934
references: Dev Cell. 2007 Feb;12(2):287-99
references: 17276345
references: Development. 2005 Nov;132(22):5043-54
references: 16236764
references: Genes Dev. 2002 May 15;16(10):1281-90
references: 12023306
references: Cold Spring Harb Symp Quant Biol. 2003;68:159-69
references: 15338614
references: Nature. 2002 Aug 29;418(6901):975-9
references: 12214599