title: Comparative characteristics of endothelial-like cells derived from human adipose mesenchymal stem cells and umbilical cord blood-derived endothelial cells.
abstract: Adult peripheral blood contains a limited number of endothelial progenitor cells that can be isolated for treatment of ischemic diseases. The adipose tissue became an interesting source of stem cells for regenerative medicine. This study aimed to investigate the phenotype of cells obtained by culturing adipose-derived mesenchymal stem cells (ad-MSCs) in the presence of endothelial growth supplements compared to endothelial cells obtained from umbilical cord blood (UCB). Passage 3 ad-MSCs and mononuclear layer from UCB were cultured in presence of endothelial growth media for 3 weeks followed by their characterization by flow cytometry and polymerase chain reaction. After culture in endothelial inductive media, ad-MSCs expressed endothelial genes and some endothelial marker proteins as CD31 and CD34, respectively. Adipose tissue could be a reliable source for easy obtaining, expanding and differentiating MSCs into endothelial-like cells for autologous cell-based therapy.
authors: Department of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, 16 Street 107, Maadi, Cairo, Egypt, tagrid8@yahoo.com.
authors: Taghrid M
authors: TM
authors: Gaafar
authors: Hala A
authors: HA
authors: Abdel Rahman
authors: Wael
authors: W
authors: Attia
authors: Hala S
authors: HS
authors: Hamza
authors: Konrad
authors: K
authors: Brockmeier
authors: Rabab E
authors: RE
authors: El Hawary
mesh_terms: D000273:Adipose Tissue; D000328:Adult; D018952:Antigens, CD34 / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D042783:Endothelial Cells / Q000737:chemistry / Q000502:physiology*; D005260:Female; D005312:Fetal Blood; D005434:Flow Cytometry; D006801:Humans; D059630:Mesenchymal Stem Cells; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000032:analysis; D016133:Polymerase Chain Reaction
chemical_list: D018952:Antigens, CD34; D015415:Biomarkers; D003470:Culture Media; D019408:Platelet Endothelial Cell Adhesion Molecule-1
references: J Surg Res. 2009 Mar;152(1):157-66
references: 19883577
references: Cell Physiol Biochem. 2011;28(1):63-76
references: 21865849
references: Exp Hematol. 2005 Nov;33(11):1402-16
references: 16263424
references: Stem Cells Int. 2012;2012:812693
references: 22577397
references: J Thromb Haemost. 2007 Apr;5(4):826-34
references: 17229052
references: Trends Cell Biol. 2002 Nov;12(11):502-8
references: 12446111
references: Discov Med. 2011 Feb;11(57):160-70
references: 21356171
references: Circulation. 2004 Feb 10;109(5):656-63
references: 14734516
references: Trends Biotechnol. 2006 Apr;24(4):150-4
references: 16488036
references: J Cardiovasc Transl Res. 2011 Apr;4(2):145-53
references: 21116883
references: Keio J Med. 2005 Sep;54(3):132-41
references: 16237275
references: Exp Biol Med (Maywood). 2008 Jul;233(7):901-13
references: 18445775
references: Methods Mol Biol. 2008;449:59-67
references: 18370083
references: Cell Biol Toxicol. 2007 Jul;23(4):223-39
references: 17370127
references: Blood. 2004 Nov 1;104(9):2752-60
references: 15226175
references: Biochem Biophys Res Commun. 2001 Nov 30;289(2):519-24
references: 11716504
references: Int J Lab Hematol. 2010 Feb;32(1 Pt 1):e40-8
references: 20088999
references: J Sex Med. 2009 Apr;6(4):967-979
references: 19207272
references: Blood. 2000 May 15;95(10):3106-12
references: 10807776
references: Mol Biol Cell. 2002 Dec;13(12):4279-95
references: 12475952
references: Aesthetic Plast Surg. 1995 Sep-Oct;19(5):421-5
references: 8526158
references: An Bras Dermatol. 2010 Sep-Oct;85(5):647-56
references: 21152789
references: PLoS One. 2012;7(10):e46842
references: 23056481
references: J Atheroscler Thromb. 2006 Apr;13(2):77-81
references: 16733294
references: Biochem Biophys Res Commun. 2006 Mar 17;341(3):882-8
references: 16442498