title: Intraocular biomarker identification in uveitis associated with juvenile idiopathic arthritis.
abstract: OBJECTIVE
  To investigate the presence of biomarkers in aqueous humor (AH) from patients with uveitis associated with juvenile idiopathic arthritis (JIA).
  
  
  METHODS
  AH (N = 73) AND SERUM (N = 105) SAMPLES FROM 116 CHILDREN WERE ANALYZED USING SURFACE ENHANCED LASER DESORPTION/IONIZATION TIME OF FLIGHT MASS SPECTROMETRY (SELDI-TOF MS).
  JIA, silent chronic anterior uveitis (AU), other uveitis entities, and noninflammatory controls. Statistical biomarker identification was performed using the SELDI-ToF Biomarker Analysis Cluster Wizard followed by multivariate statistical analysis. Biochemical identification of biomarkers was performed by polyacrylamide gel protein separation, followed by liquid chromatography tandem mass spectrometry. ELISA was performed in a number of AH samples representing all four study groups.
  
  
  RESULTS
  In the JIA group, one AH protein peak at mass/charge (m/z) 13,762 had qualitative and quantitative differences in expression compared with the other uveitis entities and the controls, but not to the group of silent chronic AU. Its quantitative expression in AH of patients with JIA and other silent chronic AU was positively associated with uveitis activity. The protein at m/z 13,762 in AH was identified as transthyretin (TTR). The TTR concentration in AH differed significantly between the study groups (P = 0.006) with considerably higher TTR concentrations in JIA and silent chronic AU samples positive for m/z 13,762 than those of the other uveitis and control groups.
  
  
  CONCLUSIONS
  TTR is a potential intraocular biomarker of JIA- associated uveitis. Its role in the pathogenesis of silent chronic AU with and without arthritis needs further investigation.
authors: Department of Ophthalmology, University Medical Center Utrecht, Utrecht, The Netherlands. v.kalininaayuso@umcutrecht.nl
authors: Viera
authors: V
authors: Kalinina Ayuso
authors: Joke H
authors: JH
authors: de Boer
authors: Helen L
authors: HL
authors: Byers
authors: Gary R
authors: GR
authors: Coulton
authors: Jojanneke
authors: J
authors: Dekkers
authors: Lenneke
authors: L
authors: de Visser
authors: Anton M
authors: AM
authors: van Loon
authors: Peter A W J F
authors: PA
authors: Schellekens
authors: Aniki
authors: A
authors: Rothova
authors: Jolanda D F
authors: JD
authors: de Groot-Mijnes
mesh_terms: D000293:Adolescent; D001082:Aqueous Humor / Q000378:metabolism*; D001171:Arthritis, Juvenile / Q000150:complications* / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D002386:Cataract / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D005260:Female; D005901:Glaucoma / Q000378:metabolism; D006801:Humans; D007223:Infant; D008297:Male; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014605:Uveitis / Q000175:diagnosis / Q000209:etiology / Q000378:metabolism; D055815:Young Adult
chemical_list: D015415:Biomarkers
keywords: aqueous humor; biomarker; juvenile idiopathic arthritis; proteins; uveitis