title: The characteristic expression pattern of BMI-1 and SALL4 genes in placenta tissue and cord blood.
abstract: BACKGROUND
  SALL4 and BMI-1 are important factors in hematopoiesis. Placental tissue (PT) and umbilical cord blood (CB) are rich in hematopoietic stem/progenitor cells (HSCs/HPCs), but their SALL4 and BMI-1 expression levels remain unknown.
  
  
  METHODS
  Real-time PCR was used to determine the expression level of these genes in PT and CB from ten cases, and ten healthy donors were used as controls.
  
  
  RESULTS
  A significantly higher BMI-1 and SALL4 gene expression level was found in PT (median: 17.548 and 34.362, respectively) than in cord blood mononuclear cells (CBMCs) (median: 2.071 and 11.300, respectively) (P = 0.0001 and P = 0.007) and healthy peripheral blood mononuclear cells (PBMCs) (median: 0.259 and 0.384, respectively) (P = 0.001 and P <0.0001), and their expression level was lower in PBMCs than in CBMCs (P = 0.029 and P = 0.002). A positive correlation between the BMI-1 and SALL4 genes was found in the PT and CB groups, while there was no significant correlation between these genes in the healthy group. There was also no significant correlation between the expression level of each gene in PT and CB.
  
  
  CONCLUSIONS
  These results describe the characteristic features of the BMI-1 and SALL4 gene expression pattern in placental tissue and cord blood. Placental tissue with higher expression level of both genes may be considered as a potential resource for SALL4-related HPC expansion.
authors: Shaohua
authors: S
authors: Chen
authors: Sichu
authors: S
authors: Liu
authors: Ling
authors: L
authors: Xu
authors: Lijian
authors: L
authors: Yang
authors: Zhenyi
authors: Z
authors: Jin
authors: Yu
authors: Y
authors: Ma
authors: Bo
authors: B
authors: Li
authors: Xiuli
authors: X
authors: Wu
authors: Jianchang
authors: J
authors: Yang
authors: Yupo
authors: Y
authors: Ma
authors: Yangqiu
authors: Y
authors: Li
mesh_terms: D005260:Female; D005312:Fetal Blood / Q000378:metabolism*; D006801:Humans; D010920:Placenta / Q000378:metabolism*; D063150:Polycomb Repressive Complex 1 / Q000235:genetics / Q000378:metabolism*; D011247:Pregnancy; D060888:Real-Time Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: C069077:BMI1 protein, human; C472575:SALL4 protein, human; D014157:Transcription Factors; D063150:Polycomb Repressive Complex 1
references: Curr Opin Hematol. 2007 Mar;14(2):162-9
references: 17255794
references: Cancer Cell Int. 2012 Oct 15;12(1):42
references: 23067006
references: Stem Cell Res Ther. 2011 Dec 29;2(6):50
references: 22206617
references: J Hematol Oncol. 2011 Jun 10;4:27
references: 21663651
references: Transfusion. 2013 May;53(5):1037-49
references: 22934838
references: Exp Hematol Oncol. 2012 May 14;1(1):12
references: 23210618
references: Nature. 2003 May 15;423(6937):302-5
references: 12714971
references: Immunity. 2004 Dec;21(6):843-51
references: 15589172
references: Proc Natl Acad Sci U S A. 2007 Jun 19;104(25):10494-9
references: 17557835
references: J Hematol Oncol. 2012 Mar 18;5:10
references: 22424172
references: Trends Immunol. 2003 Oct;24(10):522-4
references: 14552834
references: Stem Cell Res Ther. 2011 Sep 22;2(5):38
references: 21939558
references: Stem Cell Res Ther. 2011 Apr 13;2(2):19
references: 21489244
references: Stem Cell Res Ther. 2011 May 19;2(3):25
references: 21596003
references: EMBO J. 1992 Aug;11(8):2941-50
references: 1353445
references: Hematology. 2011 May;16(3):185-9
references: 21669059
references: Am J Hum Genet. 2002 Nov;71(5):1195-9
references: 12395297
references: Stem Cell Res Ther. 2012 Dec 19;3(6):57
references: 23253356