title: Story in a sample-the potential (and limitations) of cryo-electron microscopy applied to molecular machines.
abstract: This article addresses recent developments in cryo-electron microscopy and single-particle reconstruction of macromolecules. With the advent of powerful classification techniques, it is now possible to extract and visualize multiple conformers contained within the same dataset. It is discussed how and to what extent this technique can be used in the study of the dynamics of molecular machines.
authors: Department of Biochemistry and Molecular Biophysics, Howard Hughes Medical Institute, Columbia University, New York, NY, 10032; Department of Biological Sciences, Columbia University, New York, NY, 10027.
authors: Joachim
authors: J
authors: Frank
mesh_terms: D020285:Cryoelectron Microscopy; D046911:Macromolecular Substances; D012270:Ribosomes
chemical_list: D046911:Macromolecular Substances
keywords: conformational states; free-energy landscape; ribosome; single-molecule FRET
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