title: Arabidopsis CBL-interacting protein kinase (CIPK6) is involved in plant response to salt/osmotic stress and ABA.
abstract: The CBL-CIPK signaling pathway represents a central and critical signaling system involved in plant response to abiotic stress and hormone signaling. In this study, we focused on AtCIPK6 gene, which have been shown to be required for development and salt tolerance in Arabidopsis, but the transcriptional regulatory mechanism of AtCIPK6 gene and whether it plays a role in ABA signaling is still unknown. Here, we showed that in addition to its expression induced by salt and drought stress, the transcripts of AtCIPK6 gene were largely accumulated in abscisic acid treated seedling, compared to basal level expression. Moreover, promoter sequence analysis revealed that there are some elements involved in stress and hormone response detected in the promoter region of AtCIPK6. AtCIPK6p:GUS transgenic assays unraveled that AtCIPK6 promoter is salt/osmotic stress- and ABA-inducible. Overexpression of AtCIPK6 gene in Arabidopsis increased plant tolerance to salt stress, but sensitivity to ABA. Our results contribute to the understanding of transcriptional regulatory mechanism of AtCIPK6 gene and may also provide a good stress-inducible promoter candidate for transgenic engineering.
authors: Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan, 430079, China.
authors: Liang
authors: L
authors: Chen
authors: Qing-Qing
authors: QQ
authors: Wang
authors: Li
authors: L
authors: Zhou
authors: Feng
authors: F
authors: Ren
authors: Deng-Di
authors: DD
authors: Li
authors: Xue-Bao
authors: XB
authors: Li
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology*; D017360:Arabidopsis / Q000201:enzymology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D009997:Osmotic Pressure / Q000502:physiology*; D011401:Promoter Regions, Genetic / Q000187:drug effects / Q000235:genetics; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology*; D013312:Stress, Physiological / Q000235:genetics / Q000502:physiology*
chemical_list: D029681:Arabidopsis Proteins; D017931:DNA Primers; D000040:Abscisic Acid; C562040:CIPK6 protein, Arabidopsis; D011494:Protein Kinases
references: Trends Plant Sci. 2007 Aug;12(8):343-51
references: 17629540
references: Plant Mol Biol. 2012 May;79(1-2):123-35
references: 22382993
references: Plant Cell. 2002;14 Suppl:S389-400
references: 12045290
references: Plant Cell Physiol. 2004 Mar;45(3):346-50
references: 15047884
references: Cell. 2009 Sep 18;138(6):1184-94
references: 19766570
references: Plant Cell. 2002;14 Suppl:S401-17
references: 12045291
references: Science. 1990 Oct 12;250(4978):267-71
references: 2145628
references: Plant Cell. 2003 Feb;15(2):411-23
references: 12566581
references: Plant Cell. 1998 Jul;10(7):1181-91
references: 9668136
references: Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6896-901
references: 10823923
references: Proc Natl Acad Sci U S A. 2010 Apr 27;107(17):8023-8
references: 20385816
references: Plant Cell. 2006 May;18(5):1292-309
references: 16617101
references: EMBO J. 1987 Dec 20;6(13):3901-7
references: 3327686
references: Genes Dev. 2010 Aug 15;24(16):1695-708
references: 20713515
references: Plant Physiol. 2004 Jan;134(1):43-58
references: 14730064
references: Proc Natl Acad Sci U S A. 2009 Oct 13;106(41):17588-93
references: 19805022
references: Science. 2009 May 22;324(5930):1068-71
references: 19407142
references: Plant Cell. 1990 Jun;2(6):503-12
references: 2152172
references: Proc Natl Acad Sci U S A. 2003 Sep 30;100(20):11771-6
references: 14504388
references: Plant J. 2009 Jun;58(5):778-90
references: 19187042
references: Plant Physiol. 2002 May;129(1):225-34
references: 12011353
references: Plant Physiol. 2010 May;153(1):185-97
references: 20335401
references: J Exp Bot. 2012 Oct;63(17):6211-22
references: 23105131
references: Proc Natl Acad Sci U S A. 2006 Aug 15;103(33):12625-30
references: 16895985
references: Plant Biotechnol J. 2012 Sep;10(7):792-805
references: 22551450
references: Proc Natl Acad Sci U S A. 2002 Jun 11;99(12):8436-41
references: 12034882
references: Planta. 2004 Oct;219(6):915-24
references: 15322881
references: Proc Natl Acad Sci U S A. 2007 Oct 2;104(40):15959-64
references: 17898163
references: Plant Physiol. 2002 Oct;130(2):666-74
references: 12376634
references: Proc Natl Acad Sci U S A. 2002 Mar 19;99(6):4097-102
references: 11891305
references: Plant Cell. 2004 Jul;16(7):1912-24
references: 15208400
references: Plant Cell. 1998 Aug;10(8):1391-406
references: 9707537
references: Science. 2009 May 22;324(5930):1064-8
references: 19407143
references: Plant J. 2009 Jan;57(2):264-78
references: 18798873
references: Dev Cell. 2002 Aug;3(2):233-44
references: 12194854
references: EMBO J. 2001 Mar 1;20(5):1051-63
references: 11230129
references: Plant J. 2006 Dec;48(6):857-72
references: 17092313
references: Cell. 2006 Jun 30;125(7):1347-60
references: 16814720
references: Plant Cell. 2005 Mar;17(3):859-75
references: 15722467
references: Plant Cell. 2007 Apr;19(4):1415-31
references: 17449811
references: Plant J. 2007 Aug;51(4):617-30
references: 17587305
references: Nat Biotechnol. 1999 Mar;17(3):287-91
references: 10096298