title: An efficient flat-surface collar-free grafting method for Arabidopsis thaliana seedlings.
abstract: BACKGROUND
  Grafting procedures are an excellent tool to study long range signalling processes within a plant. In the last decade, suitable flat-surface grafting procedures for young Arabidopsis seedlings using a collar to support the graft have been developed, allowing the study of long-range signals from a molecular perspective.
  
  
  RESULTS
  In the modification presented here, scion and stock are put together on the medium without supporting elements, while cotyledons are removed from the scion, resulting in increased grafting success that can reach up to 100%. At the same time, the protocol enables to process as many as 36 seedlings per hour, which combined with the high success percentage represents increased efficiency per time unit.
  
  
  CONCLUSIONS
  Growing cotyledons usually push the scion and the rootstock away in the absence of a supporting element. Removing them at the grafting step greatly improved success rate and reduced post-grafting manipulations.
authors: Instituto de Ecología, UNAM, México, DF, México. gerco.angenent@wur.nl.
authors: Nayelli
authors: N
authors: Marsch-Martínez
authors: John
authors: J
authors: Franken
authors: Karla L
authors: KL
authors: Gonzalez-Aguilera
authors: Stefan
authors: S
authors: de Folter
authors: Gerco
authors: G
authors: Angenent
authors: Elena R
authors: ER
authors: Alvarez-Buylla
references: Science. 2010 May 14;328(5980):872-5
references: 20413459
references: Plant Physiol. 2004 Aug;135(4):2271-8
references: 15299137
references: Curr Biol. 2006 Mar 21;16(6):553-63
references: 16546078
references: Plant Physiol. 2006 May;141(1):108-20
references: 16531489
references: Proc Natl Acad Sci U S A. 2007 Sep 11;104(37):14741-6
references: 17785412
references: Plant Physiol. 2002 May;129(1):201-10
references: 12011351
references: Plant Methods. 2012 Dec 19;8(1):50
references: 23249585
references: Science. 2007 May 18;316(5827):1030-3
references: 17446353
references: Plant Physiol. 2012 Jul;159(3):984-1000
references: 22582134
references: Plant Cell. 2007 May;19(5):1488-506
references: 17540715
references: Plant Cell. 1997 Nov;9(11):1963-71
references: 9401121
references: Methods Mol Biol. 2010;655:11-26
references: 20734251
references: Plant J. 2002 Oct;32(2):255-62
references: 12383090
references: Methods Mol Biol. 2006;323:39-44
references: 16739566
references: Plant Cell Physiol. 2013 Jan;54(1):119-28
references: 23204014
references: BMC Plant Biol. 2010 Apr 13;10:64
references: 20388194