title: Screening and determination of drugs in human saliva utilizing microextraction by packed sorbent and liquid chromatography-tandem mass spectrometry.
abstract: This study presents a new method for collecting and handling saliva samples using an automated analytical microsyringe and microextraction by packed syringe (MEPS). The screening and determination of lidocaine in human saliva samples utilizing MEPS and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were carried out. An exact volume of saliva could be collected. The MEPS C8 -cartridge could be used for 50 extractions before it was discarded. The extraction recovery was about 60%. The pharmacokinetic curve of lidocaine in saliva using MEPS-LC-MS/MS is reported.
authors: Department of Analytical Chemistry, Stockholm University, SE10691 Stockholm, Sweden.
authors: Abbi
authors: A
authors: Abdel-Rehim
authors: Mohamed
authors: M
authors: Abdel-Rehim
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D008012:Lidocaine / Q000032:analysis / Q000302:isolation & purification; D008956:Models, Chemical; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000302:isolation & purification; D012463:Saliva / Q000737:chemistry*; D052617:Solid Phase Microextraction / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004364:Pharmaceutical Preparations; D008012:Lidocaine
keywords: LC-MS/MS; MEPS; eVol; lidocaine; saliva samples