title: Emergence of methicillin-resistant Staphylococcus aureus in the Maternity Hospital, Kuwait.
abstract: OBJECTIVE
  To establish the relatedness of methicillin-resistant Staphylococcus aureus (MRSA) isolates in the Maternity Hospital, Kuwait.
  
  
  METHODS
  A total of 22 MRSA were isolated from 20 neonates and 1 mother in the Special Care Unit, Maternity Hospital, Kuwait. They were characterized using antibiogram, pulsed-field gel electrophoresis (PFGE), SCCmec typing, spa typing and multi locus sequence typing (MLST), and were screened for genes encoding Panton Valentine leukocidin (PVL) and capsular polysaccharide types 5 and 8.
  
  
  RESULTS
  The isolates were resistant to cadmium acetate (n = 22 or 100%), trimethoprim (n = 13 or 59.1%), gentamicin (n = 7 or 31.8%), ciprofloxacin (n = 5 or 22.7%), erythromycin and clindamycin (n = 2 or 9.1%), tetracycline (n = 2 or 9.1%) and fusidic acid (n = 2 or 9.1%). Eight isolates contained genes for PVL while 15 and 6 carried genes for types 5 and 8 capsular polysaccharide, respectively. Molecular typing distinguished 12 clones. Ten of these clones consisted of 20 isolates belonging to ST60-SCCmec-IV-t3935 (5 isolates), ST6-SCCmec-IV-t6269 (4 isolates), ST194-SCCmec-IV-t6892 (3 isolates), ST1-SCCmec-V-t2962 (2 isolates) and 1 isolate each of ST77-SCCmec-IV-t339, ST935-SCCmec-V-t1084, ST1317-SCCmec-V-t1548, ST9-SCCmec-V-t5801, ST627-SCCmec-IV-t1340 and ST2148-SCCmec-IV-t2810.
  
  
  CONCLUSIONS
  The study demonstrated the emergence of MRSA including novel ST60 and ST194 clones at the Maternity Hospital in Kuwait.
authors: Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait.
authors: Edet E
authors: EE
authors: Udo
authors: Noura
authors: N
authors: Al-Sweih
mesh_terms: D015373:Bacterial Typing Techniques; D015331:Cohort Studies; D003428:Cross Infection / Q000145:classification / Q000175:diagnosis / Q000382:microbiology*; D024881:Drug Resistance, Bacterial; D005260:Female; D006771:Hospitals, Maternity; D006801:Humans; D007231:Infant, Newborn; D007730:Kuwait; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification / Q000302:isolation & purification*; D011247:Pregnancy; D013203:Staphylococcal Infections / Q000145:classification / Q000175:diagnosis / Q000382:microbiology*
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