title: RNA-binding protein Rbm47 binds to Nanog in mouse embryonic stem cells.
abstract: Embryonic stem cells (ES cells) are pluripotent cells capable for self-renewal and to differentiate to all cell types. Finding the molecular mechanisms responsible for these unique characteristics of ES cells is important. RNA-binding proteins play important roles in post-transcriptional gene regulation by binding to specific mRNA targets. In this study, we investigated the targets of RNA-binding protein Rbm47 in mouse ES cells. Overexpression of HA epitope-tagged Rbm47 in mouse ES cells followed by RNA-binding protein immunoprecipitation, and then RT-PCR analysis of co-immunoprecipitated RNA showed that Rbm47 binds to Nanog transcript in mouse ES cells and doesn't bind to Sox2 and Oct4 transcripts in these cells. This finding can give rise to reveal molecular mechanisms underlying pluripotency and stemness of ES cells and will be necessary for efficient application of these cells in regenerative medicine and tissue engineering.
authors: Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran.
authors: Meghdad
authors: M
authors: Yeganeh
authors: Ehsan
authors: E
authors: Seyedjafari
authors: Farnaz Akbari
authors: FA
authors: Kamrani
authors: Nasser
authors: N
authors: Ghaemi
mesh_terms: D000818:Animals; D053595:Embryonic Stem Cells / Q000378:metabolism*; D015870:Gene Expression; D005786:Gene Expression Regulation; D023061:Gene Order; D005822:Genetic Vectors / Q000235:genetics; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D011485:Protein Binding; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D055748:SOXB1 Transcription Factors / Q000378:metabolism
chemical_list: D018398:Homeodomain Proteins; D000071317:Nanog Homeobox Protein; C475540:Nanog protein, mouse; D050814:Octamer Transcription Factor-3; D016601:RNA-Binding Proteins; C585951:Rbm47 protein, mouse; D055748:SOXB1 Transcription Factors
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