title: Evaluation of PCR-ELISA as a tool for monitoring transmission of Wuchereria bancrofti in District of Gampaha, Sri Lanka.
abstract: OBJECTIVE
  To compare Wuchereria bancrofti (W. bancrofti) infection rates of Culex quinquefasciatus, using dissection and PCR-ELISA in two consecutive time periods (from 2007 to 2008 and from 2008 to 2009).
  
  
  METHODS
  Mosquitoes were collected in 30 sentinel and 15 non-sentinel sites in 15 Medical Officer of Health areas of Gampaha District known for the presence of W. bancrofti transmission in two consecutive time period of 2007 to 2008 and 2008 to 2009. Captured mosquitoes were dissected to determine the W. bancrofti larvae (L1, L2, L3). PCR was carried out using DNA extracted from mosquito pools (15 body parts/pool) utilizing the primers specific for Wb-SspI repeat. PCR products were analyzed by hybridization ELISA using fluorescein-labeled wild type specific probes. The prevalence of infected/infective mosquitoes in PCR pools (3 pools/site) was estimated using the PoolScreen™ algorithm and a novel probability-based method.
  
  
  RESULTS
  Of 45 batches of mosquitoes dissected, W. bancrofti infected mosquitoes were found in 19 and 13 batches, with an infection rate of 13.29% and 3.10% with mean larval density of 8.7 and 1.0 larvae per mosquito for two study periods in the Gampaha District. Total of 405 pools of head, thorax and abdomen were processed by PCR-ELISA for each year. Of these, 51 and 31 pools were positive for W. bancrofti in the two study periods respectively. The association of dissection based prevalence rates with PCR based rates as determined by the Pearson correlation coefficient were 0.176 and 0.890 respectively for the two periods.
  
  
  CONCLUSIONS
  Data indicate that PCR-ELISA is more sensitive than the traditional dissection techniques for monitoring transmission intensity.
authors: Molecular Medicine Unit, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka.
authors: Asha Dilrukshi
authors: AD
authors: Wijegunawardana
authors: Nilmini Silva
authors: NS
authors: Gunawardane
authors: Chanditha
authors: C
authors: Hapuarachchi
authors: Aresha
authors: A
authors: Manamperi
authors: Kithsiri
authors: K
authors: Gunawardena
authors: Wimaladharma
authors: W
authors: Abeyewickrama
authors: Baha
authors: B
authors: Latif
mesh_terms: D000818:Animals; D009033:Culicidae / Q000469:parasitology; D004605:Elephantiasis, Filarial / Q000453:epidemiology* / Q000635:transmission*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D016133:Polymerase Chain Reaction; D011159:Population Surveillance; D015995:Prevalence; D013188:Sri Lanka / Q000453:epidemiology; D014958:Wuchereria bancrofti / Q000235:genetics* / Q000276:immunology*
keywords: Culex quinquefasciatus; Dissection; PCR-ELISA; Sri Lanka; Wuchereria bancrofti
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