title: Development of rapid phenotypic system for the identification of Gram-negative oxidase-positive bacilli in resource-limited settings.
abstract: Rapid and accurate identification of bacterial pathogens is a fundamental goal of clinical microbiology. The diagnosis and surveillance of diseases is dependent, to a great extent, on laboratory services, which cannot function without effective reliable reagents and diagnostics. Despite the advancement in microbiology diagnosis globally, resourcelimited countries still struggle to provide an acceptable diagnosis quality which helps in clinical disease management and improve their mortality and morbidity data. During this study an indigenous product, Quick Test Strip (QTS) NE, was developed for the rapid identification of biochemically slower group of Gram-negative oxidase-positive bacilli that covers 19 different bacterial genera. Some of the members belonging to these groups are well-established human pathogens, e.g. various species of Vibrio, Pseudomonas, Burkholderia, Aeromonas, Achromobacter and Stenotrophomonas. This study also evaluates the performance of QTS-NE by comparing with genotypic characterization methods. A total of 232 clinical and reference bacterial isolates were tested by three different methods. QTSNE provides 100 percent concordant results with other rapid identification and molecular characterization methods and confirms the potential to be used in clinical diagnosis.
authors: Immunology and Infectious Diseases Research Laboratory, Department of Microbiology, University of Karachi, University Road, Karachi 75270, Pakistan.
authors: Mahmooda
authors: M
authors: Kazmi
authors: Adnan
authors: A
authors: Khan
authors: Shahana Urooj
authors: SU
authors: Kazmi
mesh_terms: D001424:Bacterial Infections / Q000175:diagnosis* / Q000382:microbiology; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D015373:Bacterial Typing Techniques / Q000191:economics / Q000592:standards*; D006090:Gram-Negative Bacteria / Q000145:classification / Q000201:enzymology* / Q000235:genetics; D006295:Health Resources; D006801:Humans; D010088:Oxidoreductases / Q000737:chemistry* / Q000378:metabolism; D010154:Pakistan; D010641:Phenotype; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011933:Reagent Kits, Diagnostic / Q000191:economics / Q000592:standards*; D012015:Reference Standards; D012680:Sensitivity and Specificity
chemical_list: D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D011933:Reagent Kits, Diagnostic; D010088:Oxidoreductases
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