title: A novel and stable mouse artificial chromosome vector.
abstract: Human chromosome fragments (hCFs) and human artificial chromosomes (HACs) can be transferred into mouse ES cells to produce trans-chromosomic (Tc) mice. Although hCFs and HACs containing large genomic DNAs can be autonomously maintained in Tc mice, their retention rate is variable in mouse ES cell lines and Tc mouse tissues, possibly because of centromere differences between the species. To improve the retention rate of artificial chromosomes in mouse cells, we constructed novel mouse artificial chromosome (MAC) vectors by truncating a natural mouse chromosome at a site adjacent to the centromeric region. We obtained cell clones containing the MAC vectors that were stably maintained in mouse ES cells and various tissues in Tc mice. The MACs possess acceptor sites into which a desired gene or genes can be inserted. Thus, Tc mice harboring the MAC vectors may be valuable tools for functional analyses of desired genes, producing humanized model mice, and synthetic biology.
authors: Department of Biomedical Science, Institute of Regenerative Medicine and Biofunction, Graduate School of Medical Science, Tottori University , 86 Nishi-cho, Yonago, Tottori 683-8503, Japan.
authors: Masato
authors: M
authors: Takiguchi
authors: Yasuhiro
authors: Y
authors: Kazuki
authors: Kei
authors: K
authors: Hiramatsu
authors: Satoshi
authors: S
authors: Abe
authors: Yuichi
authors: Y
authors: Iida
authors: Shoko
authors: S
authors: Takehara
authors: Tadashi
authors: T
authors: Nishida
authors: Tetsuya
authors: T
authors: Ohbayashi
authors: Teruhiko
authors: T
authors: Wakayama
authors: Mitsuo
authors: M
authors: Oshimura
mesh_terms: D000818:Animals; D016466:CHO Cells; D022201:Chromosomes, Artificial / Q000235:genetics*; D006224:Cricetinae; D003412:Cricetulus; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D051379:Mice; D014162:Transfection
keywords: chromosome engineering; human artificial chromosome (HAC); humanized model mouse; mouse artificial chromosome (MAC); trans-chromosomic mouse