title: NMDA-dependent phase synchronization between septal and temporal CA3 hippocampal networks.
abstract: Increasing evidence suggests that synchronization between brain regions is essential for information exchange and memory processes. However, it remains incompletely known which synaptic mechanisms contribute to the process of synchronization. Here, we investigated whether NMDA receptor-mediated synaptic plasticity was an important player in synchronization between septal and temporal CA3 areas of the rat hippocampus. We found that both the septal and temporal CA3 regions intrinsically generate weakly synchronized δ frequency oscillations in the complete hippocampus in vitro. Septal and temporal oscillators differed in frequency, power, and rhythmicity, but both required GABAA and AMPA receptors. NMDA receptor activation, and most particularly the NR2B subunit, contributed considerably more to rhythm generation at the temporal than the septal region. Brief activation of NMDA receptors by application of extracellular calcium dramatically potentiated the septal-temporal coherence for long durations (>40 min), an effect blocked by the NMDA antagonist AP-5. This long-lasting NMDA-receptor-dependent increase in coherence was also associated with an elevated phase locking of spikes locally and across regions. Changes in coherence between oscillators were associated with increases in phase locking between oscillators independent of oscillator amplitude. Finally, although the septal CA3 rhythm preceded the oscillations in temporal regions in control conditions, this was reversed during the NMDA-dependent enhancement in coherence, suggesting that NMDA receptor activation can change the direction of information flow along the septotemporal CA3 axis. These data demonstrate that plastic changes in communication between septal and temporal hippocampal regions can arise from the NMDA-dependent phase locking of neural oscillators.
authors: McGill University, Douglas Mental Health University Institute, Montreal, Quebec H4H 1R3, Canada.
authors: Ning
authors: N
authors: Gu
authors: Jesse
authors: J
authors: Jackson
authors: Romain
authors: R
authors: Goutagny
authors: Germaine
authors: G
authors: Lowe
authors: Frédéric
authors: F
authors: Manseau
authors: Sylvain
authors: S
authors: Williams
mesh_terms: D000200:Action Potentials / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D000831:Animals, Newborn; D001640:Bicuculline / Q000494:pharmacology; D001683:Biological Clocks / Q000187:drug effects / Q000502:physiology*; D056654:CA3 Region, Hippocampal / Q000166:cytology* / Q000502:physiology; D002118:Calcium / Q000378:metabolism; D004558:Electric Stimulation; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D005260:Female; D058787:GABA-A Receptor Antagonists / Q000494:pharmacology; D066298:In Vitro Techniques; D008297:Male; D016202:N-Methylaspartate / Q000378:metabolism*; D009415:Nerve Net / Q000187:drug effects / Q000502:physiology*; D018408:Patch-Clamp Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism
chemical_list: D018691:Excitatory Amino Acid Antagonists; D058787:GABA-A Receptor Antagonists; D016194:Receptors, N-Methyl-D-Aspartate; D016202:N-Methylaspartate; D002118:Calcium; D001640:Bicuculline
references: J Physiol Pharmacol. 2011 Aug;62(4):473-82
references: 22100849
references: J Neurosci Methods. 2010 Sep 30;192(1):146-51
references: 20637804
references: J Neurosci. 2004 May 19;24(20):4767-77
references: 15152037
references: Neurobiol Learn Mem. 2007 Sep;88(2):177-85
references: 17540583
references: Proc Natl Acad Sci U S A. 2002 Mar 5;99(5):3194-8
references: 11867718
references: Neuron. 2006 Dec 7;52(5):871-82
references: 17145507
references: Trends Cogn Sci. 2005 Oct;9(10):474-80
references: 16150631
references: Neuroscience. 1994 Oct;62(4):1033-47
references: 7845584
references: Neuroscience. 2005;135(3):765-79
references: 16154282
references: J Physiol. 2006 Oct 15;576(Pt 2):457-76
references: 16887877
references: Hippocampus. 2000;10(2):187-97
references: 10791841
references: Neuron. 2010 Jan 14;65(1):7-19
references: 20152109
references: J Neurosci. 2009 Jun 24;29(25):8094-102
references: 19553449
references: J Comp Neurol. 1977 Mar 1;172(1):49-84
references: 65364
references: Neuron. 2011 Oct 6;72(1):153-65
references: 21982376
references: Proc Natl Acad Sci U S A. 2002 Aug 6;99(16):10825-30
references: 12149439
references: Neuroscience. 1990;38(3):609-19
references: 2270136
references: Neuron. 2012 Aug 9;75(3):410-7
references: 22884325
references: Eur J Neurosci. 2006 Apr;23(8):2185-96
references: 16630065
references: Front Behav Neurosci. 2012 Jan 05;5:90
references: 22319482
references: Trends Cogn Sci. 2007 Oct;11(10):442-50
references: 17905642
references: Neuroscience. 1989;31(3):571-91
references: 2687721
references: Neuron. 2010 Jun 24;66(6):921-36
references: 20620877
references: Nature. 1999 Feb 4;397(6718):430-3
references: 9989408
references: J Physiol. 1998 Feb 15;507 ( Pt 1):13-24
references: 9490809
references: J Comp Neurol. 1994 Jan 8;339(2):181-208
references: 8300905
references: Science. 2002 Jul 12;297(5579):211-8
references: 12040087
references: J Neurosci. 2010 Feb 3;30(5):1777-87
references: 20130187
references: J Neurophysiol. 2013 Jan;109(2):570-9
references: 23114214
references: Science. 2007 Mar 23;315(5819):1719-22
references: 17379810
references: J Pharmacol Exp Ther. 1997 Dec;283(3):1285-92
references: 9400004
references: J Neurosci. 2009 Jun 24;29(25):8215-24
references: 19553461
references: Neuroscience. 1982 Jun;7(6):1411-6
references: 6126840
references: J Neurophysiol. 2009 Mar;101(3):1192-200
references: 19118111
references: Nat Rev Neurosci. 2011 Feb;12(2):105-18
references: 21248789
references: Nature. 2010 Apr 1;464(7289):763-7
references: 20360742
references: J Neurosci. 2011 Aug 24;31(34):12104-17
references: 21865453
references: Science. 2007 Jun 15;316(5831):1609-12
references: 17569862
references: Behav Brain Res. 2003 Feb 17;139(1-2):197-213
references: 12642189
references: Nat Neurosci. 2009 Dec;12(12):1491-3
references: 19881503
references: Proc Natl Acad Sci U S A. 2007 Mar 27;104(13):5644-9
references: 17372196
references: J Neurosci. 2005 Apr 13;25(15):3962-72
references: 15829648
references: Neurosci Lett. 1987 Jun 1;77(1):37-42
references: 3601214
references: J Neurosci. 2006 Jun 7;26(23):6213-29
references: 16763029
references: Nat Neurosci. 2013 Mar;16(3):349-56
references: 23354333
references: Neuron. 2011 Sep 8;71(5):898-910
references: 21903082
references: Science. 2006 Sep 15;313(5793):1626-8
references: 16973878
references: Hum Brain Mapp. 1999;8(4):194-208
references: 10619414
references: Neuron. 2005 Apr 7;46(1):141-51
references: 15820700
references: Nature. 1999 Sep 2;401(6748):63-9
references: 10485705
references: J Neurosci. 1993 Sep;13(9):3916-25
references: 8366351
references: Brain Res Bull. 1986 Sep;17(3):291-6
references: 3021291
references: Eur J Pharmacol. 2010 Jan 10;626(1):49-56
references: 19836379
references: Proc Natl Acad Sci U S A. 2010 Apr 6;107(14):6516-9
references: 20332204
references: Hippocampus. 1998;8(6):608-19
references: 9882018
references: Science. 2004 May 14;304(5673):1021-4
references: 15143284
references: Nature. 1989 Nov 9;342(6246):175-7
references: 2812013
references: J Neurophysiol. 2010 Aug;104(2):1195-210
references: 20463205
references: J Neurosci. 1999 May 15;19(10):4180-8
references: 10234045
references: J Physiol. 2010 Jan 1;588(Pt 1):93-9
references: 19822542
references: J Neurophysiol. 2010 Aug;104(2):994-1006
references: 20505130
references: Curr Opin Neurobiol. 2012 Jun;22(3):496-508
references: 22325859
references: Trends Neurosci. 2007 Jul;30(7):365-73
references: 17559951
references: Behav Neurosci. 1999 Dec;113(6):1170-88
references: 10636297
references: Neuron. 2009 Oct 29;64(2):267-80
references: 19874793
references: Science. 2003 Aug 8;301(5634):846-50
references: 12907806
references: PLoS Biol. 2009 Apr 21;7(4):e1000089
references: 19385719