title: Remarkable impact of PAHs and TPHs on the richness and diversity of bacterial species in surface soils exposed to long-term hydrocarbon pollution.
abstract: Nowadays, because of substantial use of petroleum-derived fuels the number and extension of hydrocarbon polluted terrestrial ecosystems is in growth worldwide. In remediation of aforementioned sites bioremediation still tends to be an innovative, environmentally attractive technology. Although huge amount of information is available concerning the hydrocarbon degradation potential of cultivable hydrocarbonoclastic bacteria little is known about the in situ long-term effects of petroleum derived compounds on the structure of soil microbiota. Therefore, in this study our aim was to determine the long-term impact of total petroleum hydrocarbons (TPHs), volatile petroleum hydrocarbons (VPHs), total alkyl benzenes (TABs) as well as of polycyclic aromatic hydrocarbons (PAHs) on the structure of bacterial communities of four different contaminated soil samples. Our results indicated that a very high amount of TPH affected positively the diversity of hydrocarbonoclastic bacteria. This finding was supported by the occurrence of representatives of the α-, β-, γ-Proteobacteria, Actinobacteria, Flavobacteriia and Bacilli classes. High concentration of VPHs and TABs contributed to the predominance of actinobacterial isolates. In PAH impacted samples the concentration of PAHs negatively correlated with the diversity of bacterial species. Heavily PAH polluted soil samples were mainly inhabited by the representatives of the β-, γ-Proteobacteria (overwhelming dominance of Pseudomonas sp.) and Actinobacteria.
authors: Department of Analytical Chemistry and Environmental Engineering, Polytehnical University of Bucharest, Splaiul Independenţei 313, 060042, Bucharest, Romania, benedektibor@sapientia.siculorum.ro.
authors: Tibor
authors: T
authors: Benedek
authors: Balázs
authors: B
authors: Vajna
authors: András
authors: A
authors: Táncsics
authors: Károly
authors: K
authors: Márialigeti
authors: Szabolcs
authors: S
authors: Lányi
authors: István
authors: I
authors: Máthé
mesh_terms: D039903:Actinobacteria / Q000235:genetics / Q000302:isolation & purification*; D001555:Benzene Derivatives / Q000737:chemistry / Q000378:metabolism; D001673:Biodegradation, Environmental; D044822:Biodiversity; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D017753:Ecosystem; D019143:Evolution, Molecular; D006838:Hydrocarbons / Q000737:chemistry* / Q000378:metabolism; D064307:Microbiota; D010578:Petroleum / Q000378:metabolism; D010802:Phylogeny; D011084:Polycyclic Aromatic Hydrocarbons / Q000737:chemistry / Q000378:metabolism; D020560:Proteobacteria / Q000235:genetics / Q000302:isolation & purification*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000737:chemistry*; D013045:Species Specificity
chemical_list: D001555:Benzene Derivatives; D004269:DNA, Bacterial; D006838:Hydrocarbons; D010578:Petroleum; D011084:Polycyclic Aromatic Hydrocarbons; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants
references: J Environ Manage. 2012 Mar;95 Suppl:S325-31
references: 21802196
references: Chemosphere. 2004 Nov;57(5):401-12
references: 15331267
references: Appl Environ Microbiol. 2004 Jun;70(6):3425-33
references: 15184140
references: Microb Ecol. 2010 Apr;59(3):445-56
references: 19789910
references: Sci Total Environ. 2010 Sep 15;408(20):4334-40
references: 20655572
references: Environ Pollut. 2005 Jan;133(1):71-84
references: 15327858
references: Appl Environ Microbiol. 1997 Nov;63(11):4516-22
references: 9361437
references: Appl Environ Microbiol. 2009 May;75(9):2613-20
references: 19270134
references: ISME J. 2008 Sep;2(9):968-81
references: 18563190
references: J Environ Qual. 2005 May 11;34(3):1073-80
references: 15888893
references: Biochem Biophys Res Commun. 2002 Jun 14;294(3):560-6
references: 12056803
references: FEMS Microbiol Ecol. 2005 Nov 1;54(3):375-80
references: 16332335
references: Mar Pollut Bull. 2010 Nov;60(11):2056-61
references: 20719344
references: Environ Microbiol. 2006 Mar;8(3):455-65
references: 16478452
references: Can J Microbiol. 2003 Aug;49(8):508-13
references: 14608386
references: Int J Syst Evol Microbiol. 2001 Jul;51(Pt 4):1405-17
references: 11491340
references: PLoS One. 2011 Mar 02;6(3):e16943
references: 21399677
references: Appl Environ Microbiol. 2003 Jan;69(1):275-84
references: 12514005
references: Sci Total Environ. 2012 Jun 1;426:56-64
references: 22542235
references: Microb Ecol. 2009 Jul;58(1):153-60
references: 18958515
references: J Hazard Mater. 2009 Sep 30;169(1-3):1-15
references: 19442441
references: Appl Environ Microbiol. 1993 Mar;59(3):695-700
references: 7683183
references: Mol Biol Evol. 2007 Aug;24(8):1596-9
references: 17488738
references: J Environ Sci Health A Tox Hazard Subst Environ Eng. 2011;46(3):281-90
references: 21308599
references: Microb Ecol. 2012 Oct;64(3):702-13
references: 22614940
references: Chemosphere. 2007 Jul;68(8):1535-41
references: 17482237
references: Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
references: 9254694
references: Res Microbiol. 2005 Mar;156(2):201-10
references: 15748985
references: J Environ Manage. 2012 May 30;99:10-7
references: 22306081
references: Int J Syst Evol Microbiol. 2007 Oct;57(Pt 10):2259-2261
references: 17911292
references: Environ Sci Pollut Res Int. 2012 Jan;19(1):31-41
references: 21647694