title: Exposure to phthalic acid, phthalate diesters and phthalate monoesters from foodstuffs: UK total diet study results.
abstract: Phthalates are ubiquitous in the environment and thus exposure to these compounds can occur in various forms. Foods are one source of such exposure. There are only a limited number of studies that describe the levels of phthalates (diesters, monoesters and phthalic acid) in foods and assess the exposure from this source. In this study the levels of selected phthalate diesters, phthalate monoesters and phthalic acid in total diet study (TDS) samples are determined and the resulting exposure estimated. The methodology for the determination of phthalic acid and nine phthalate monoesters (mono-isopropyl phthalate, mono-n-butyl phthalate, mono-isobutyl phthalate, mono-benzyl phthalate, mono-cyclohexyl phthalate, mono-n-pentyl phthalate, mono-(2-ethylhexyl) phthalate, mono-n-octyl phthalate and mono-isononyl phthalate) in foods is described. In this method phthalate monoesters and phthalic acid are extracted from the foodstuffs with a mixture of acidified acetonitrile and dichloromethane. The method uses isotope-labelled phthalic acid and phthalate monoester internal standards and is appropriate for quantitative determination in the concentration range of 5-100 µg kg⁻¹. The method was validated in-house and its broad applicability demonstrated by the analysis of high-fat, high-carbohydrate and high-protein foodstuffs as well as combinations of all three major food constituents. The methodology used for 15 major phthalate diesters has been reported elsewhere. Phthalic acid was the most prevalent phthalate, being detected in 17 food groups. The highest concentration measured was di-(2-ethylhexyl) phthalate in fish (789 µg kg⁻¹). Low levels of mono-n-butyl phthalate and mono-(2-ethylhexyl) phthalate were detected in several of the TDS animal-based food groups and the highest concentrations measured corresponded with the most abundant diesters (di-n-butyl phthalate and di-(2-ethylhexyl) phthalate). The UK Committee on Toxicity of Chemicals in Food, Consumer Products and the Environment (COT) considered the levels found and concluded that they did not indicate a risk to human health from dietary exposure alone.
authors: The Food and Environment Research Agency, York, UK. emma.bradley@fera.gsi.gov.uk
authors: Emma L
authors: EL
authors: Bradley
authors: Richard A
authors: RA
authors: Burden
authors: Karim
authors: K
authors: Bentayeb
authors: Malcolm
authors: M
authors: Driffield
authors: Nick
authors: N
authors: Harmer
authors: David N
authors: DN
authors: Mortimer
authors: Dennis R
authors: DR
authors: Speck
authors: Jana
authors: J
authors: Ticha
authors: Laurence
authors: L
authors: Castle
mesh_terms: D002853:Chromatography, Liquid; D004032:Diet; D004952:Esters / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D010795:Phthalic Acids / Q000032:analysis* / Q000737:chemistry; D053719:Tandem Mass Spectrometry; D006113:United Kingdom
chemical_list: D004952:Esters; D010795:Phthalic Acids