title: Overexpression of β-actin is closely associated with metastasis of gastric cancer.
abstract: OBJECTIVE
  β-actin is known to be involved in motility of cancer cells. However, the association between β-actin expression and metastasis of gastric cancer remains unclear.
  
  
  METHODS
  We enrolled 40 gastric cancer patients who underwent gastrectomy and evaluated the expression of β-actin in gastric cancer tissues and the corresponding normal mucosa by the analysis of Western blotting. To further study the function of β-actin in cancer cell migration, wound healing assay was performed in SGC7901 transfected with β-actin siRNA.
  
  
  RESULTS
  The level of β-actin was significantly higher in gastric cancer tissues than in the corresponding normal mucosa. The correlation between β-actin expression and the local lymph node metastasis was statistically significant, which demonstrated that overexpression of β-actin might contribute to the local lymph node metastasis of gastric cancer. Interference of β-actin expression could inhibit migration of SGC7901 cells.
  
  
  CONCLUSIONS
  β-actin, the most conservative and dynamic protein, is overexpressed in gastric cancer tissues and may play a crucial role in metastasis of gastric cancer.
authors: GuangDong Provincial Key Laboratory of Gastroenterology, Nanfang Hospital, Southern Medical University, GuangDong, China.
authors: Jing
authors: J
authors: Xu
authors: Zhenshu
authors: Z
authors: Zhang
authors: Jianting
authors: J
authors: Chen
authors: Fuqiang
authors: F
authors: Liu
authors: Lan
authors: L
authors: Bai
mesh_terms: D000199:Actins / Q000235:genetics / Q000378:metabolism*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement; D005260:Female; D005743:Gastrectomy; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D034622:RNA Interference; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000556:secondary* / Q000601:surgery; D014162:Transfection; D015854:Up-Regulation
chemical_list: D000199:Actins