title: Long-term potentiation of synaptic transmission in the adult mouse insular cortex: multielectrode array recordings.
abstract: The insular cortex (IC) is widely believed to be an important forebrain structure involved in cognitive and sensory processes such as memory and pain. However, little work has been performed at the cellular level to investigate the synaptic basis of IC-related brain functions. To bridge the gap, the present study was designed to characterize the basic synaptic mechanisms for insular long-term potentiation (LTP). Using a 64-channel recording system, we found that an enduring form of late-phase LTP (L-LTP) could be reliably recorded for at least 3 h in different layers of IC slices after theta burst stimulation. The induction of insular LTP is protein synthesis dependent and requires activation of both GluN2A and GluN2B subunits of the NMDA receptor, L-type voltage-gated calcium channels, and metabotropic glutamate receptor 1. The paired-pulse facilitation ratio was unaffected by insular L-LTP induction, and expression of insular L-LTP required the recruitment of postsynaptic calcium-permeable AMPA receptors. Our results provide the first in vitro report of long-term multichannel recordings of L-LTP in the IC in adult mice and suggest its potential important roles in insula-related memory and chronic pain.
authors: Department of Brain and Cognitive Sciences, College of Natural Sciences, Seoul National University, Seoul, Korea.
authors: Ming-Gang
authors: MG
authors: Liu
authors: Sukjae Joshua
authors: SJ
authors: Kang
authors: Tian-Yao
authors: TY
authors: Shi
authors: Kohei
authors: K
authors: Koga
authors: Ming-Ming
authors: MM
authors: Zhang
authors: Graham L
authors: GL
authors: Collingridge
authors: Bong-Kiun
authors: BK
authors: Kaang
authors: Min
authors: M
authors: Zhuo
mesh_terms: D000818:Animals; D000841:Anisomycin / Q000494:pharmacology; D002540:Cerebral Cortex / Q000502:physiology*; D066298:In Vitro Techniques; D017774:Long-Term Potentiation / Q000187:drug effects / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009482:Neurophysiology / Q000379:methods; D018091:Receptors, AMPA / Q000502:physiology; D016194:Receptors, N-Methyl-D-Aspartate / Q000502:physiology; D009435:Synaptic Transmission / Q000502:physiology
chemical_list: D018091:Receptors, AMPA; D016194:Receptors, N-Methyl-D-Aspartate; D000841:Anisomycin
keywords: calcium-permeable AMPA receptor; glutamate receptor; insular cortex; long-term potentiation; multielectrode array
references: Cell. 1994 Oct 7;79(1):69-79
references: 7923379
references: Dev Neurobiol. 2007 Mar;67(4):498-509
references: 17443804
references: Eur J Neurosci. 2002 Sep;16(6):1139-45
references: 12383243
references: Brain Res. 2003 Nov 21;991(1-2):274-9
references: 14575905
references: J Neurosci. 1997 Jul 1;17(13):5129-35
references: 9185550
references: Mol Pain. 2007 Apr 30;3:11
references: 17470281
references: J Neurosci. 2012 Aug 15;32(33):11318-29
references: 22895715
references: Nat Neurosci. 2006 May;9(5):602-4
references: 16582904
references: Curr Pharm Des. 2005;11(21):2797-807
references: 16101456
references: J Neurosci. 2008 Jun 4;28(23):6000-9
references: 18524905
references: Mol Brain. 2009 Feb 03;2:4
references: 19192303
references: EMBO J. 2007 Nov 28;26(23):4879-90
references: 17972919
references: Front Syst Neurosci. 2011 Sep 26;5:79
references: 21991247
references: Trends Neurosci. 2007 Mar;30(3):126-34
references: 17275103
references: J Neurophysiol. 1999 Dec;82(6):2956-69
references: 10601432
references: Science. 2006 Aug 25;313(5790):1093-7
references: 16931756
references: Neuroimage. 2005 Aug 1;27(1):201-9
references: 15921935
references: J Neurosci. 2005 Jul 20;25(29):6907-10
references: 16033900
references: PLoS One. 2010 Sep 29;5(9):
references: 20927382
references: Neuropharmacology. 2004 Mar;46(3):311-7
references: 14975686
references: Science. 2010 Dec 3;330(6009):1400-4
references: 21127255
references: J Neurosci. 1999 Nov 1;19(21):RC36
references: 10531470
references: Science. 2000 Oct 20;290(5491):533-6
references: 11039938
references: Mol Brain. 2010 Sep 16;3:27
references: 20846411
references: Science. 2010 Nov 19;330(6007):1108-12
references: 21030604
references: Pain. 2007 Mar;128(1-2):20-30
references: 17011704
references: Mol Cells. 2007 Jun 30;23(3):259-71
references: 17646700
references: Behav Brain Res. 2002 Feb 1;129(1-2):101-6
references: 11809500
references: Nature. 1993 Jan 7;361(6407):31-9
references: 8421494
references: Hippocampus. 2006;16(11):907-15
references: 17024679
references: Proc Natl Acad Sci U S A. 1991 Jun 15;88(12):5379-82
references: 2052615
references: Brain Res. 1998 Oct 26;809(1):91-6
references: 9795158
references: Neurobiol Learn Mem. 2011 May;95(4):519-26
references: 21440652
references: Exp Neurobiol. 2012 Jun;21(2):37-51
references: 22792024
references: Mol Brain. 2012 Sep 18;5:31
references: 22986281
references: J Neurosci. 2003 Jul 23;23(16):6557-66
references: 12878697
references: Neuron. 2007 Jun 21;54(6):859-71
references: 17582328
references: Prog Neurobiol. 1999 Sep;59(1):55-79
references: 10416961
references: PLoS One. 2009;4(2):e4339
references: 19190753
references: Neuropsychopharmacology. 2008 Jan;33(1):18-41
references: 17728696
references: J Neurosci. 1997 Jul 1;17(13):5196-205
references: 9185557
references: J Neurosci. 2007 Apr 25;27(17):4598-602
references: 17460072
references: Ann N Y Acad Sci. 2011 Apr;1225:72-82
references: 21534994
references: J Neurosci. 2000 Sep 15;20(18):7017-23
references: 10995847
references: Neuropsychopharmacology. 2008 Apr;33(5):1004-18
references: 17625504
references: Pain. 2011 Mar;152(3 Suppl):S49-S64
references: 21146929
references: Mol Brain. 2012 Feb 03;5:6
references: 22304729
references: Science. 2001 Mar 23;291(5512):2417-9
references: 11264539
references: Prog Neurobiol. 2003 Feb;69(3):143-79
references: 12758108
references: Nat Rev Neurosci. 2004 Mar;5(3):209-17
references: 14976520
references: Nature. 1997 Dec 11;390(6660):604-7
references: 9403688
references: Neurosci Lett. 2002 Apr 5;322(2):87-90
references: 11958850
references: Trends Neurosci. 2008 Apr;31(4):199-207
references: 18329111
references: Eur J Neurosci. 2005 Jul;22(2):485-94
references: 16045501
references: Brain Res Brain Res Rev. 1999 Jan;29(1):83-120
references: 9974152
references: J Physiol. 2013 Feb 15;591(4):955-72
references: 23230236
references: Brain Res. 2000 Jan 3;852(1):208-12
references: 10661514
references: J Neurosci. 2007 Mar 21;27(12):3111-9
references: 17376972
references: Curr Opin Neurobiol. 1999 Jun;9(3):299-304
references: 10395580
references: Neurosci Lett. 2012 Jan 17;507(1):38-42
references: 22172929
references: Mol Brain. 2008 Sep 30;1:9
references: 18826591
references: Learn Mem. 2005 Sep-Oct;12(5):447-9
references: 16166398
references: Nature. 1999 Sep 2;401(6748):63-9
references: 10485705
references: J Neurophysiol. 1998 May;79(5):2535-45
references: 9582226
references: Neuropharmacology. 2005;49 Suppl 1:13-25
references: 16024054
references: Eur J Neurosci. 2006 Sep;24(5):1434-42
references: 16965548
references: J Neurosci. 2004 Sep 8;24(36):7821-8
references: 15356193
references: J Neurosci. 1996 May 15;16(10):3189-98
references: 8627357
references: J Neurosci. 1999 Nov 1;19(21):9346-54
references: 10531439
references: J Neurosci. 2000 Sep 1;20(17):6317-25
references: 10964936
references: Science. 2004 May 14;304(5673):1021-4
references: 15143284
references: Front Behav Neurosci. 2012 Jan 05;5:87
references: 22319481
references: Neuroimage. 2006 Aug 1;32(1):256-65
references: 16679031
references: J Neurosci. 2009 Jul 22;29(29):9219-26
references: 19625512
references: Brain Res. 1998 Jan 1;779(1-2):314-9
references: 9473708
references: J Clin Invest. 2011 Apr;121(4):1608-23
references: 21383497
references: Neuroreport. 1997 Feb 10;8(3):687-93
references: 9106748
references: Nat Neurosci. 2002 Jun;5(6):573-9
references: 12006982
references: Curr Opin Neurobiol. 2011 Apr;21(2):291-8
references: 21295464
references: Neuron. 2005 Sep 15;47(6):859-72
references: 16157280
references: J Neurophysiol. 2012 Oct;108(7):1988-98
references: 22786952
references: Neuropharmacology. 2007 Jan;52(1):60-70
references: 16904707
references: Nature. 1994 Nov 17;372(6503):237-43
references: 7969468