title: Myriocin, a serine palmitoyltransferase inhibitor, blocks cytokinesis in Leishmania (Viannia) braziliensis promastigotes.
abstract: We studied the effect of myriocin, an inhibitor of serine palmitoyltransferase, on cultured Leishmania (Viannia) braziliensis promastigotes. Myriocin significantly reduced synthesis of inositol phosphorylceramide, the major sphingolipid expressed in promastigotes as characterized by thin layer chromatography and electrospray ionization mass spectrometry. Log-phase promastigotes treated with 1 μM myriocin showed a 52% reduction in growth rate and morphological alterations such as more rounded shape and shorter flagellum. Promastigotes treated with myriocin also displayed a variety of aberrant cell phenotypes. The percentage of cells with one nucleus and one kinetoplast (1N1K), following treatment with 1 or 5 μM myriocin, decreased from 89% (control value) to 27% or 3%, respectively. The percentage of cells with two nuclei (2N2K) varied from 7% (control value) to 19% and 6% for 1 or 5 μM myriocin-treated parasites, respectively. High percentage of myriocin-treated parasites exhibited large atypical cells presenting three or more nucleus (32% and 89% for 1 or 5 μM myriocin, respectively). Transmission electron microscopy following treatment with 1 μM myriocin showed the presence of 4N parasites possibly as a result of an incomplete cytokinesis. Addition of 3-ketodihidrosphingosine to myriocin-treated promastigotes rescue parasite growth and morphology. Addition of ethanolamine did not rescue the myriocin effect on parasite. Our findings indicate that sphingolipids are essential for the completion of cytokinesis, and may play a major role in cell proliferation in L. (V.) braziliensis, thus, differing from data described for Leishmania major sphingolipid-free mutant, where addition of ethanolamine rescue wild-type parasite characteristics.
authors: Department of Biochemistry, Escola Paulista de Medicina, Universidade Federal de São Paulo, Rua Botucatu 862, São Paulo, SP 04023-900, Brazil.
authors: Erica V
authors: EV
authors: Castro
authors: Kelly G
authors: KG
authors: Yoneyama
authors: Edna F
authors: EF
authors: Haapalainen
authors: Marcos S
authors: MS
authors: Toledo
authors: Helio K
authors: HK
authors: Takahashi
authors: Anita H
authors: AH
authors: Straus
mesh_terms: D048749:Cytokinesis / Q000187:drug effects*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005229:Fatty Acids, Monounsaturated / Q000494:pharmacology*; D019084:Fluorescent Antibody Technique, Indirect; D006028:Glycosphingolipids / Q000378:metabolism; D007892:Leishmania braziliensis / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D051102:Serine C-Palmitoyltransferase / Q000037:antagonists & inhibitors*; D013107:Sphingolipids / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D005229:Fatty Acids, Monounsaturated; D006028:Glycosphingolipids; D013107:Sphingolipids; C477937:inositolphosphorylceramide; D051102:Serine C-Palmitoyltransferase; C001996:thermozymocidin
keywords: inositol phosphorylceramide; sphingolipid