title: Rat subcutaneous tissue response to MTA Fillapex® and Portland cement.
abstract: The aim of this study was to evaluate the response of rat subcutaneous tissue to MTA Fillapex® (Angelus), an experimental root canal filling material based on Portland cement and propylene glycol (PCPG), and a zinc oxide, eugenol and iodoform (ZOEI) paste. These materials were placed in polyethylene tubes and implanted into the dorsal connective tissue of Wistar rats for 7 and 15 days. The specimens were stained with hematoxylin and eosin, and evaluated regarding inflammatory reaction parameters by optical microscopy. The intensity of inflammatory response against the sealers was analyzed by two blinded and previously calibrated examiners for all experimental periods (kappa=0.96). The histological evaluation showed that all materials caused a moderate inflammatory reaction at 7 days, which subsided with time. A greater inflammatory reaction was observed at 7 days in the tubes filled with ZOEI paste. Tubes filled with MTA Fillapex presented some giant cells, macrophages and lymphocytes after 7 days. At 15 days, the presence of fibroblasts and collagen fibers was observed indicating normal tissue healing. The tubes filled with PCPG showed similar results to those observed in MTA Fillapex. At 15 days, the inflammatory reaction was almost absent at the tissue, with several collagen fibers indicating normal tissue healing. Data were analyzed by the nonparametric Kruskal-Wallis test (α=0.05). Statistically significant difference (p<0.05) was found only between PCPG at 15 days and ZOEI at 7 days groups. No significant differences were observed among the other groups/periods (p>0.05). MTA Fillapex and Portland cement added with propylene glycol had greater tissue compatibility than the PCPG paste.
authors: Department of Pediatric Dentistry,Orthodontics and Public Health, Bauru Dental School, University of São Paulo, Bauru, SP, Brazil.
authors: Nádia Carolina Teixeira
authors: NC
authors: Marques
authors: Natalino
authors: N
authors: Lourenço Neto
authors: Ana Paula
authors: AP
authors: Fernandes
authors: Camila de Oliveira
authors: Cde O
authors: Rodini
authors: Marco Antônio Hungaro
authors: MA
authors: Duarte
authors: Thais Marchini
authors: TM
authors: Oliveira
mesh_terms: D017607:Aluminum Compounds / Q000633:toxicity*; D000818:Animals; D017610:Calcium Compounds / Q000633:toxicity*; D003238:Connective Tissue / Q000187:drug effects; D003738:Dental Cements / Q000633:toxicity*; D004338:Drug Combinations; D006847:Hydrocarbons, Iodinated / Q000633:toxicity; D008297:Male; D008422:Materials Testing; D010087:Oxides / Q000633:toxicity*; D019946:Propylene Glycol; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012387:Root Canal Filling Materials / Q000737:chemistry / Q000633:toxicity*; D017640:Silicates / Q000633:toxicity*; D040521:Subcutaneous Tissue / Q000187:drug effects*; D015035:Zinc Oxide-Eugenol Cement / Q000633:toxicity*
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D003738:Dental Cements; D004338:Drug Combinations; D006847:Hydrocarbons, Iodinated; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; D015035:Zinc Oxide-Eugenol Cement; C472247:accelerated Portland cement; C086631:mineral trioxide aggregate; D019946:Propylene Glycol; C010473:iodoform