title: Rapid research response to the 2009 A(H1N1)pdm09 influenza pandemic (Revised).
abstract: BACKGROUND
  When novel influenza viruses cause human infections, it is critical to characterize the illnesses, viruses, and immune responses to infection in order to develop diagnostics, treatments, and vaccines. The objective of the study was to collect samples from patients with suspected or confirmed A(H1N1)pdm09 infections that could be made available to the scientific community. Respiratory secretions, sera and peripheral blood mononuclear cells (PBMCs) were collected sequentially (when possible) from patients presenting with suspected or previously confirmed A(H1N1)pdm09 infections. Clinical manifestations and illness outcomes were assessed. Respiratory secretions were tested for the presence of A(H1N1)pdm09 virus by means of isolation in tissue culture and real time RT-PCR. Sera were tested for the presence and level of HAI and neutralizing antibodies against the A(H1N1)pdm09 virus.
  
  
  CONCLUSIONS
  Thirty patients with confirmed A(H1N1)pdm09 infection were enrolled at Baylor College of Medicine (BCM). Clinical manifestations of illness were consistent with typical influenza. Twenty-eight of 30 had virological confirmation of illness; all recovered fully. Most patients had serum antibody responses or high levels of antibody in convalescent samples. Virus-positive samples were sent to J. Craig Venter Institute for sequencing and sequences were deposited in GenBank. Large volumes of sera collected from 2 convalescent adults were used to standardize antibody assays; aliquots of these sera are available from the repository. Aliquots of serum, PBMCs and stool collected from BCM subjects and subjects enrolled at other study sites are available for use by the scientific community, upon request.
authors: Department of Molecular Virology & Microbiology, Houston, TX, USA. wkeitel@bcm.edu
authors: Wendy A
authors: WA
authors: Keitel
authors: Pedro A
authors: PA
authors: Piedra
authors: Robert L
authors: RL
authors: Atmar
authors: Gail
authors: G
authors: Demmler
authors: Hana M
authors: HM
authors: El Sahly
authors: Jill
authors: J
authors: Barrett
authors: Rebecca A
authors: RA
authors: Halpin
authors: Rosanna
authors: R
authors: Lagos
authors: Susan
authors: S
authors: Fisher-Hoch
authors: Flor
authors: F
authors: Munoz
mesh_terms: D000293:Adolescent; D057134:Antibodies, Neutralizing / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D007251:Influenza, Human / Q000453:epidemiology* / Q000821:virology; D008297:Male; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D057134:Antibodies, Neutralizing
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