title: A blue-absorbing photolabile protecting group for in vivo chromatically orthogonal photoactivation.
abstract: The small and synthetically easily accessible 7-diethylamino-4-thiocoumarinylmethyl photolabile protecting group has been validated for uncaging with blue light. It exhibits a significant action cross-section for uncaging in the 470-500 nm wavelength range and a low light absorption between 350 and 400 nm. These attractive features have been implemented in living zebrafish embryos to perform chromatic orthogonal photoactivation of two biologically active species controlling biological development with UV and blue-cyan light sources, respectively.
authors: Ecole Normale Supérieure, Département de Chimie, UMR CNRS-ENS-UPMC 8640 PASTEUR , 24, rue Lhomond, 75231 Paris Cedex 05, France.
authors: Ludovic
authors: L
authors: Fournier
authors: Carole
authors: C
authors: Gauron
authors: Lijun
authors: L
authors: Xu
authors: Isabelle
authors: I
authors: Aujard
authors: Thomas
authors: T
authors: Le Saux
authors: Nathalie
authors: N
authors: Gagey-Eilstein
authors: Sylvie
authors: S
authors: Maurin
authors: Sylvie
authors: S
authors: Dubruille
authors: Jean-Bernard
authors: JB
authors: Baudin
authors: David
authors: D
authors: Bensimon
authors: Michel
authors: M
authors: Volovitch
authors: Sophie
authors: S
authors: Vriz
authors: Ludovic
authors: L
authors: Jullien
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000737:chemistry*; D002219:Carbamates / Q000737:chemistry*; D003116:Color; D008027:Light; D018613:Microscopy, Confocal; D015394:Molecular Structure; D013194:Staining and Labeling; D015027:Zebrafish / Q000196:embryology
chemical_list: D001559:Benzhydryl Compounds; D002219:Carbamates