title: Simultaneous Detection of Mixed Infection of Onion yellow dwarf virus and an Allexivirus in RT-PCR for Ensuring Virus Free Onion Bulbs.
abstract: Reduced seed production in onion is associated with Onion yellow dwarf virus (OYDV), a filamentous Potyvirus. Onion is also infected with other filamentous virus particles suspected to be Allexivirus. RT-PCR was used to detect mixed infection of both the viruses in leaves and bulbs. A duplex RT-PCR was developed, which simultaneously detected the presence of these two viruses in winter (Rabi) onion bulb. In summer (Kharif) onion bulbs only Allexivirus was detected. The absence of OYDV in summer crop is discussed. The sequencing of RT-PCR amplified products confirmed the identity of OYDV and Allexivirus, the latter showing closer identity to Garlic virus C (GVC)/Garlic mite-borne mosaic virus. This makes the first detection of an Allexivirus in onion crop in India. The duplex RT-PCR to detect these viruses (OYDV and Allexivirus) would be an improvement for indexing of viruses in onion bulbs for seed production.
authors: Plant Virology Unit, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110012 India.
authors: Sandeep
authors: S
authors: Kumar
authors: V K
authors: VK
authors: Baranwal
authors: Subodh
authors: S
authors: Joshi
authors: Meenakshi
authors: M
authors: Arya
authors: S
authors: S
authors: Majumder
keywords: Allexivirus; Leaf; Onion bulb; Onion yellow dwarf virus; Virus indexing
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