title: Inhibition of Dengue virus 2 replication by artificial micrornas targeting the conserved regions.
abstract: Dengue virus (DENV), a mosquito-borne flavivirus, causes serious diseases and threatens public health in tropical and subtropical areas worldwide. RNA interference (RNAi) is a prevailing strategy for antiviral therapy. In this paper, 6 single artificial microRNAs (amiRNAs) targeting the highly conserved regions of the DENV-2 genome were identified and inhibited virus replication efficiently. Then, effective tandem amiRNAs targeting 2 different DENV-2 genome regions were constructed and expressed simultaneously from a single microRNA-like polycistron to avoid virus variation or mutation escape. Finally, the most high-performance tandem amiRNA was embedded in a lenti-viral vector and inhibited DENV-2 virus replication stably and dose-dependently. Overall, these results indicated that RNAi based on multiple amiRNAs targeting viral conserved regions was an effective approach for improvements of nucleic acid inhibitors of DENV and provided a new therapeutic strategy for DENV infection in humans.
authors: Department of Biotechnology, Beijing Institute of Radiation Medicine, Beijing 100850, China.
authors: Pei-wen
authors: PW
authors: Xie
authors: Yu
authors: Y
authors: Xie
authors: Xiu-juan
authors: XJ
authors: Zhang
authors: Hai
authors: H
authors: Huang
authors: Li-na
authors: LN
authors: He
authors: Xue-jun
authors: XJ
authors: Wang
authors: Sheng-qi
authors: SQ
authors: Wang
mesh_terms: D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D017124:Conserved Sequence / Q000235:genetics; D006224:Cricetinae; D003716:Dengue Virus / Q000235:genetics* / Q000254:growth & development; D018390:Gene Targeting / Q000379:methods*; D005822:Genetic Vectors; D016679:Genome, Viral; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D051379:Mice; D035683:MicroRNAs / Q000138:chemical synthesis / Q000235:genetics*; D008969:Molecular Sequence Data; D034622:RNA Interference; D014779:Virus Replication
chemical_list: D035683:MicroRNAs
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