title: Simultaneous determination of three Curcuminoids in Curcuma wenyujin Y.H.chen et C.Ling. by liquid chromatography-tandem mass spectrometry combined with pressurized liquid extraction.
abstract: A sensitive and efficient method based on pressurized liquid extraction (PLE) followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for simultaneous identification and quantification of three Curcuminoids in Curcuma wenyujin Y.H.chen et C.Ling., a well known traditional Chinese medicinal herb. PLE was performed using methanol as the extractant, and with the temperature set at 100 °C and the pressure at 1500 psi. Shorter extraction time and less solvent consumption were obtained compared to other extraction methods. LC was performed by using a Waters XBridge C18 column (150 mm × 2.1 mm i.d., 3.5 μm) and a mobile phase of acetonitrile containing 0.1% acetic acid. The operating parameters were thoroughly optimized. The recoveries ranged from 92.4% to 104.1% and relative standard deviations were lower than 3.6%. The method was applied to the determination of Curcumin, Demethoxycurcumin and Bisdemethoxycurcumin contained in Curcuma wenyujin Y.H.chen et C.Ling. samples, which provided a new analytical approach for analysis and quality assessment of traditional Chinese medicines.
authors: Analytical & Testing Center, Sichuan University, Chengdu 610064, China.
authors: Yan
authors: Y
authors: Shen
authors: Chao
authors: C
authors: Han
authors: Xiangzhun
authors: X
authors: Chen
authors: Xiandeng
authors: X
authors: Hou
authors: Zhou
authors: Z
authors: Long
mesh_terms: D002853:Chromatography, Liquid; D030024:Curcuma / Q000737:chemistry*; D003474:Curcumin / Q000031:analogs & derivatives / Q000032:analysis* / Q000302:isolation & purification; D036381:Diarylheptanoids; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000737:chemistry; D059625:Liquid-Liquid Extraction / Q000379:methods*; D012997:Solvents / Q000737:chemistry; D053719:Tandem Mass Spectrometry; D013997:Time Factors
chemical_list: D036381:Diarylheptanoids; D004365:Drugs, Chinese Herbal; D012997:Solvents; C034786:bisdemethoxycurcumin; D003474:Curcumin; C050229:demethoxycurcumin