title: Modulated crosslinking of macroporous polymeric cryogel affects in vitro cell adhesion and growth.
abstract: Cell to matrix interactions affect in vitro cell adherence and proliferation and further decide the fate of tissue development for biomedical applications. This study demonstrates the role of crosslinking in altering the surface properties of 3D porous cryogel matrices. Glutaraldehyde and 1-ethyl-3(3-dimethylaminopropyl)-carbodiimide crosslinkers are used separately for the crosslinking of alginate and gelatin (i.e., AG(G) and AG(EN)), respectively. The difference in crosslinking affects the physiochemical properties of these matrices leading to variable cell behavior as demonstrated using four different cell types, which show homogeneous cell growth in AG(G) and spheroid cell growth in AG(EN). The present study shows successful use of controlled crosslinking in directing the cell growth for tissue engineering.
authors: Department of Biological Sciences and Bioengineering, Indian Institute of Technology Kanpur, Kanpur-208016, UP, India.
authors: Anuj
authors: A
authors: Tripathi
authors: Tanushree
authors: T
authors: Vishnoi
authors: Deepti
authors: D
authors: Singh
authors: Ashok
authors: A
authors: Kumar
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion; D049109:Cell Proliferation; D059985:Cryogels / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D016062:Porosity; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D059985:Cryogels; D020100:Hydrogels; D011108:Polymers; D005780:Gelatin
keywords: Cell-matrix interaction; crosslinkers; cryogels; stem cell; tissue-engineering