title: Imaging DNA with fluorochrome bearing metals.
abstract: Molecules that fluoresce upon binding DNA are widely used in assaying and visualizing DNA in cells and tissues. However, using light to visualize DNA in animals is limited by the attenuation of light transmission by biological tissues. Moreover, it is now clear that DNA is an important mediator of dead cell clearance, coagulation reactions, and an immunogen in autoimmune lupus. Attaching metals (e.g., superparamagnetic nanoparticles, gadolinium ions, radioactive metal ions) to DNA-binding fluorochromes provides a way of imaging DNA in whole animals, and potentially humans, without light. Imaging metal-bearing, DNA-binding fluorochromes and their target DNA by magnetic resonance imaging may shed light on the many key roles of DNA in health and disease beyond the storage of genetic information.
authors: Center for Translational Nuclear Medicine and Molecular Imaging, ‡Martinos Center for Biomedical Imaging, Department of Radiology, and §Cardiovascular Research Center, Cardiology Division, Massachusetts General Hospital, Harvard Medical School , Boston, Massachusetts 02114, United States.
authors: Hoonsung
authors: H
authors: Cho
authors: Yanyan
authors: Y
authors: Guo
authors: David E
authors: DE
authors: Sosnovik
authors: Lee
authors: L
authors: Josephson
mesh_terms: D000818:Animals; D003550:Cystic Fibrosis / Q000503:physiopathology; D004247:DNA / Q000032:analysis* / Q000502:physiology; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D005682:Gadolinium / Q000737:chemistry; D006801:Humans; D007364:Intercalating Agents / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry; D008670:Metals / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods*; D015899:Tomography, Emission-Computed, Single-Photon / Q000379:methods; D051598:Whole Body Imaging / Q000379:methods
chemical_list: D005456:Fluorescent Dyes; D007364:Intercalating Agents; D008670:Metals; D004247:DNA; D005682:Gadolinium
references: Cytometry. 1994 Feb 1;15(2):129-40
references: 7513274
references: Biochim Biophys Acta. 1988 Feb 28;949(2):158-68
references: 2449244
references: J Thromb Haemost. 2012 Jan;10(1):136-44
references: 22044575
references: Anal Biochem. 1982 Apr;121(2):382-7
references: 6179438
references: J Magn Reson. 2009 Dec;201(2):175-85
references: 19786359
references: Proc Natl Acad Sci U S A. 1990 Dec;87(23):9188-92
references: 2251263
references: Expert Rev Respir Med. 2007 Dec;1(3):317-29
references: 20477171
references: Nat Med. 2008 Sep;14(9):914-5
references: 18776881
references: Circ Cardiovasc Imaging. 2011 Nov;4(6):729-37
references: 21836081
references: Methods Mol Biol. 2011;726:33-49
references: 21424441
references: Angew Chem Int Ed Engl. 2012 Jul 9;51(28):6904-7
references: 22684680
references: Biochemistry. 1978 Nov 28;17(24):5078-88
references: 569495
references: Trends Biotechnol. 2007 Oct;25(10):433-6
references: 17825446
references: J Immunol. 2005 Jun 1;174(11):7352-8
references: 15905582
references: ACS Nano. 2013 Mar 26;7(3):2032-41
references: 23373524
references: N Engl J Med. 1994 Sep 8;331(10):637-42
references: 7503821
references: Anticancer Res. 2007 May-Jun;27(3A):1257-65
references: 17593617
references: Ann N Y Acad Sci. 2003 Dec;1010:467-70
references: 15033771
references: Cancer Treat Rev. 2002 Oct;28(5):255-71
references: 12435372
references: Autoimmunity. 2007 Jun;40(4):281-4
references: 17516210
references: Proc Natl Acad Sci U S A. 1990 May;87(10):3851-5
references: 2339125
references: Eur Respir J. 1990 Jan;3(1):19-23
references: 2107097
references: J Am Chem Soc. 2007 Feb 21;129(7):2025-34
references: 17256855
references: N Engl J Med. 2008 Feb 28;358(9):929-39
references: 18305268
references: Nature. 1992 Oct 29;359(6398):859-61
references: 1436062
references: Proc Soc Exp Biol Med. 1957 Dec;96(3):572-4
references: 13505795
references: Thorax. 1999 May;54(5):470
references: 10409074
references: Clin Chem. 2000 Mar;46(3):319-23
references: 10702517
references: Science. 2004 Mar 5;303(5663):1532-5
references: 15001782
references: Ann N Y Acad Sci. 2001 Sep;945:211-20
references: 11708481
references: Cell. 2010 Mar 5;140(5):619-30
references: 20211132
references: Lab Chip. 2005 Mar;5(3):280-4
references: 15726204
references: Appl Microbiol Biotechnol. 2006 Dec;73(3):495-504
references: 17063328
references: Arthritis Res Ther. 2013 Jan 22;15(1):102
references: 23347779
references: J Clin Microbiol. 1990 Mar;28(3):495-503
references: 1691208
references: Hum Genet. 1977 May 10;36(3):299-305
references: 67077
references: Arthritis Res Ther. 2012 Jul 27;14(4):R174
references: 22838636
references: J Vasc Interv Radiol. 2012 May;23(5):712-8
references: 22525027
references: Arthritis Rheum. 1964 Aug;7:379-90
references: 14202576
references: Cancer Res. 2010 Jan 15;70(2):560-7
references: 20068178
references: Chem Commun (Camb). 2009 Aug 7;(29):4444-6
references: 19597620
references: Nat Med. 2008 Aug;14(8):869-74
references: 18607350
references: Anal Chem. 2009 Jul 15;81(14):5637-45
references: 19522527
references: Angew Chem Int Ed Engl. 1998 Nov 2;37(20):2754-2794
references: 29711117
references: Proc Natl Acad Sci U S A. 2010 Sep 7;107(36):15880-5
references: 20798043
references: Arthritis Rheum. 2012 Nov;64(11):3677-86
references: 23112091
references: Ann N Y Acad Sci. 2010 Oct;1209:10-6
references: 20958310