title: Fabrication and in vitro evaluation of stable collagen/hyaluronic acid biomimetic multilayer on titanium coatings.
abstract: Layer-by-layer (LBL) self-assembly technique has been proved to be a highly effective method to immobilize the main components of the extracellular matrix such as collagen and hyaluronic acid on titanium-based implants and form a polyelectrolyte multilayer (PEM) film by electrostatic interaction. However, the formed PEM film is unstable in the physiological environment and affects the long-time effectiveness of PEM film. In this study, a modified LBL technology has been developed to fabricate a stable collagen/hyaluronic acid (Col/HA) PEM film on titanium coating (TC) by introducing covalent immobilization. Scanning electron microscopy, diffuse reflectance Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy were used to characterize the PEM film. Results of Sirius red staining demonstrated that the chemical stability of PEM film was greatly improved by covalent cross-linking. Cell culture assays further illustrated that the functions of human mesenchymal stem cells, such as attachment, spreading, proliferation and differentiation, were obviously enhanced by the covalently immobilized Col/HA PEM on TCs compared with the absorbed Col/HA PEM. The improved stability and biological properties of the Col/HA PEM covalently immobilized TC may be beneficial to the early osseointegration of the implants.
authors: Shanghai Institute of Ceramics, Chinese Academy of Science, Shanghai, People's Republic of China.
authors: Haiyong
authors: H
authors: Ao
authors: Youtao
authors: Y
authors: Xie
authors: Honglue
authors: H
authors: Tan
authors: Shengbing
authors: S
authors: Yang
authors: Kai
authors: K
authors: Li
authors: Xiaodong
authors: X
authors: Wu
authors: Xuebin
authors: X
authors: Zheng
authors: Tingting
authors: T
authors: Tang
mesh_terms: D000704:Analysis of Variance; D001391:Azo Compounds; D032701:Biomimetics; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D003094:Collagen / Q000737:chemistry*; D005109:Extracellular Matrix / Q000737:chemistry*; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D008855:Microscopy, Electron, Scanning; D056951:Photoelectron Spectroscopy; D019736:Prostheses and Implants; D060888:Real-Time Polymerase Chain Reaction; D017550:Spectroscopy, Fourier Transform Infrared; D055672:Static Electricity; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D001391:Azo Compounds; C062246:Direct Red 81; D006820:Hyaluronic Acid; D003094:Collagen; D014025:Titanium
keywords: covalent immobilization; extracellular matrix; layer-by-layer; osteogenic activity; titanium coating
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