title: Three-phase partitioning as a rapid and easy method for the purification and recovery of catalase from sweet potato tubers (Solanum tuberosum).
abstract: Three-phase partitioning (TPP) was used to purify and recover catalase from potato crude extract. The method consists of ammonium sulfate saturation, t-butanol addition, and adjustment of pH, respectively. The best catalase recovery (262 %) and 14.1-fold purification were seen in the interfacial phase in the presence of 40 % (w/v) ammonium sulfate saturation with 1.0:1.0 crude extract/t-butanol ratio (v/v) at pH 7 in a single step. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the enzyme showed comparatively purification and protein molecular weight was nearly found to be 56 kDa. This study shows that TPP is a simple, economical, and quick method for the recovering of catalase and can be used for the purification process.
authors: Section of Biochemistry, Department of Chemistry, Faculty of Arts and Sciences, Kocaeli University, Umuttepe Central Campus, 41380 İzmit, Kocaeli, Turkey. yonca.avciduman@gmail.com
authors: Yonca Avcı
authors: YA
authors: Duman
authors: Erdem
authors: E
authors: Kaya
mesh_terms: D002374:Catalase / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification*; D004789:Enzyme Activation; D004795:Enzyme Stability; D059625:Liquid-Liquid Extraction / Q000379:methods*; D008970:Molecular Weight; D035281:Plant Tubers / Q000201:enzymology*; D011198:Solanum tuberosum / Q000201:enzymology*
chemical_list: D002374:Catalase