title: X-ray structure analysis and characterization of AFUEI, an elastase inhibitor from Aspergillus fumigatus.
abstract: Elastase from Aspergillus sp. is an important factor for aspergillosis. AFUEI is an inhibitor of the elastase derived from Aspergillus fumigatus. AFUEI is a member of the I78 inhibitor family and has a high inhibitory activity against elastases of Aspergillus fumigatus and Aspergillus flavus, human neutrophil elastase and bovine chymotrypsin, but does not inhibit bovine trypsin. Here we report the crystal structure of AFUEI in two crystal forms. AFUEI is a wedge-shaped protein composed of an extended loop and a scaffold protein core. The structure of AFUEI shows remarkable similarity to serine protease inhibitors of the potato inhibitor I family, although they are classified into different inhibitor families. A structural comparison with the potato I family inhibitors suggests that the extended loop of AFUEI corresponds to the binding loop of the potato inhibitor I family, and AFUEI inhibits its cognate proteases through the same mechanism as the potato I family inhibitors.
authors: Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-Ku, Nagoya 464-8602, Japan.
authors: Mayuko
authors: M
authors: Sakuma
authors: Katsumi
authors: K
authors: Imada
authors: Yoshiyuki
authors: Y
authors: Okumura
authors: Kei-ichi
authors: K
authors: Uchiya
authors: Nobuo
authors: N
authors: Yamashita
authors: Kenji
authors: K
authors: Ogawa
authors: Atsushi
authors: A
authors: Hijikata
authors: Tsuyoshi
authors: T
authors: Shirai
authors: Michio
authors: M
authors: Homma
authors: Toshiaki
authors: T
authors: Nikai
mesh_terms: D000595:Amino Acid Sequence; D001232:Aspergillus fumigatus; D018360:Crystallography, X-Ray; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D006860:Hydrogen Bonding; D019272:Leukocyte Elastase / Q000037:antagonists & inhibitors / Q000737:chemistry; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D017386:Sequence Homology, Amino Acid; D015842:Serine Proteinase Inhibitors / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D005656:Fungal Proteins; D015842:Serine Proteinase Inhibitors; D019272:Leukocyte Elastase
keywords: Aspergillus; Protease; Protease Inhibitor; Protein Structure; Serine Protease
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