title: NFYA1 is involved in regulation of postgermination growth arrest under salt stress in Arabidopsis.
abstract: The nuclear factor Y (NF-Y), which is a ubiquitous transcription factor found in eukaryotes, is composed of three distinct subunits, namely, NF-YA, NF-YB, and NF-YC. Here, we firstly characterized the detailed function of the Arabidopsis NFYA1 factor. It is found that the 35S::AtNFYA1-overexpressed lines were hypersensitive to salt stress and Abscisic acid (ABA) during the early-postgermination growth stages. The transgenic lines exhibited a severe postgermination growth arrest compared with the wild-type (WT) under salt stress and ABA treatment. Interestingly, sodium tungstate, which is an ABA synthesis inhibitor, restored the salt-sensitive phenotype of the 35S::AtNFYA1 lines. Results of the qRT-PCR analysis showed that the mRNA levels of ABI3 and ABI5, as well as their downstream genes AtEM1 and AtEM6, were more greatly upregulated under salt stress during seed germination in the transgenic lines compared with those in WT. On the other hand, the NFYA1-RNAi lines were found to be insensitive to salt stress and exhibited decreased levels of ABI3, ABI5, EM1, and EM6 transcripts. Our results provide clear evidence supporting a role of AtNFYA1 in regulating postgermination growth arrest under salt stress.
authors: State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian, Shandong, People's Republic of China.
authors: Yan-Jie
authors: YJ
authors: Li
authors: Yi
authors: Y
authors: Fang
authors: Ya-Ru
authors: YR
authors: Fu
authors: Jin-Guang
authors: JG
authors: Huang
authors: Chang-Ai
authors: CA
authors: Wu
authors: Cheng-Chao
authors: CC
authors: Zheng
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism / Q000494:pharmacology; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism; D053898:Biosynthetic Pathways; D023081:CCAAT-Binding Factor / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D009154:Mutation; D030821:Plants, Genetically Modified; D034622:RNA Interference; D055049:Salt Tolerance / Q000235:genetics*; D015398:Signal Transduction; D013312:Stress, Physiological / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: C091601:ABI3 protein, Arabidopsis; C409743:ABI5 protein, Arabidopsis; D029681:Arabidopsis Proteins; D050976:Basic-Leucine Zipper Transcription Factors; D023081:CCAAT-Binding Factor; D014157:Transcription Factors; C402244:nuclear factor Y; D000040:Abscisic Acid
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