title: A major quantitative trait locus for cold-responsive gene expression is linked to frost-resistance gene Fr-A2 in common wheat.
abstract: Low temperature induces expression of Cor (cold-responsive)/Lea (late embryogenesis-abundant) gene family members through C-repeat binding factor (CBF) transcription factors in common wheat. However, the relationship between the genetic loci controlling cold-responsive gene expression and freezing tolerance is unclear. In expression quantitative trait locus (eQTL) analysis, accumulated transcripts of Cor/Lea and CBF genes were quantified in recombinant inbred lines derived from a cross between two common wheat cultivars with different levels of freezing tolerance. Four eQTLs controlling five cold-responsive genes were found, and the major eQTL with the greatest effect was located on the long arm of chromosome 5A. At least the 1D and 5A eQTLs played important roles in development of freezing tolerance in common wheat. The chromosomal location of the 5A eQTL, controlling four cold-responsive genes, coincided with a region homoeologous to a frost-tolerance locus (Fr-A (m) 2) reported as a CBF cluster region in einkorn wheat. The 5A eQTL plays a significant role through Cor/Lea gene expression in cold acclimation of wheat. In addition, our results suggest that one or more CBF copies at the Fr-2 region positively regulate other copies, which might amplify the positive effects of the CBF cluster on downstream Cor/Lea gene activation.
authors: Graduate School of Agricultural Science, Kobe University , 1-1 Rokkodai, Nada, Kobe, Hyogo 657-8501, Japan.
authors: Yoichi
authors: Y
authors: Motomura
authors: Fuminori
authors: F
authors: Kobayashi
authors: Julio C M
authors: JC
authors: Iehisa
authors: Shigeo
authors: S
authors: Takumi
keywords: Triticum aestivum L.; cold acclimation; freezing tolerance; recombinant inbred lines; transcript accumulation
references: Plant J. 2002 Aug;31(3):279-92
references: 12164808
references: Theor Appl Genet. 2010 Aug;121(4):629-41
references: 20401645
references: Plant Mol Biol. 2008 Jun;67(3):257-70
references: 18317935
references: Plant J. 2007 Jul;51(2):308-21
references: 17559507
references: Plant Biotechnol J. 2011 Feb;9(2):230-49
references: 20642740
references: J Exp Bot. 2005 Mar;56(413):887-95
references: 15668223
references: Funct Integr Genomics. 2009 Nov;9(4):513-23
references: 19488798
references: BMC Genomics. 2010 Nov 11;11:629
references: 21070652
references: Theor Appl Genet. 1995 Jun;90(7-8):1174-9
references: 24173081
references: PLoS One. 2010 Jan 06;5(1):e8598
references: 20066049
references: Plant Physiol. 2003 Dec;133(4):1755-67
references: 14645724
references: J Plant Physiol. 2010 Mar 15;167(5):343-50
references: 19962784
references: J Exp Bot. 2008;59(4):891-905
references: 18326864
references: Plant Physiol. 2005 Oct;139(2):643-51
references: 16219925
references: Physiol Plant. 2004 Apr;120(4):585-594
references: 15032820
references: Funct Integr Genomics. 2007 Jan;7(1):53-68
references: 16775685
references: Theor Appl Genet. 2004 Feb;108(4):670-80
references: 14576984
references: Trends Plant Sci. 2011 Sep;16(9):481-8
references: 21700486
references: Physiol Plant. 2008 Sep;134(1):74-86
references: 18433415
references: Plant Mol Biol. 2005 Nov;59(4):533-51
references: 16244905
references: Theor Appl Genet. 2007 Nov;115(8):1083-91
references: 17763839
references: J Exp Bot. 2001 Dec;52(365):2367-74
references: 11709586
references: Annu Rev Plant Physiol Plant Mol Biol. 1999 Jun;50:571-599
references: 15012220
references: Hereditas. 2012 Apr;149(2):62-71
references: 22568701
references: Mol Genet Genomics. 2007 May;277(5):533-54
references: 17285309
references: Nat Genet. 2009 Feb;41(2):166-7
references: 19169256
references: BMC Genomics. 2011 Jun 07;12:299
references: 21649926
references: Plant Physiol. 2011 May;156(1):20-8
references: 21415278
references: Genes Genet Syst. 2006 Apr;81(2):77-91
references: 16755132
references: Mol Genet Genomics. 2010 Apr;283(4):351-63
references: 20179969
references: Mol Genet Genomics. 2005 Mar;273(1):54-65
references: 15690172
references: Theor Appl Genet. 2009 May;118(8):1465-76
references: 19277599
references: Theor Appl Genet. 2006 Mar;112(5):832-42
references: 16365758
references: Genes Genet Syst. 2005 Jun;80(3):185-97
references: 16172531
references: Plant Physiol Biochem. 2008 Feb;46(2):205-11
references: 18061465
references: Mol Genet Genomics. 2005 Dec;274(5):506-14
references: 16200412
references: Mol Genet Genomics. 2003 Apr;269(1):60-7
references: 12715154
references: Plant Cell. 2011 Apr;23(4):1249-63
references: 21467582
references: Mol Gen Genet. 2000 Mar;263(2):194-200
references: 10778737
references: Trends Plant Sci. 2008 Feb;13(2):72-7
references: 18262820
references: Plant Biotechnol J. 2010 Sep;8(7):749-71
references: 20561247
references: Theor Appl Genet. 2009 Nov;119(7):1335-48
references: 19756475
references: Plant Mol Biol. 1994 Mar;24(5):701-13
references: 8193295
references: Genes Genet Syst. 2000 Feb;75(1):49-57
references: 10846621
references: J Exp Bot. 2003 Oct;54(391):2265-74
references: 12909691
references: Plant Cell. 1994 Feb;6(2):251-64
references: 8148648
references: Plant Biotechnol J. 2007 May;5(3):442-53
references: 17388792
references: Transgenic Res. 2008 Oct;17(5):755-67
references: 18034365
references: J Plant Physiol. 2003 Feb;160(2):193-200
references: 12685035
references: Mol Genet Genomics. 2006 Feb;275(2):193-203
references: 16362370