title: Improvement of ethanol production from D-lactic acid by constitutive expression of lactate transporter Jen1p in Saccharomyces cerevisiae.
abstract: To improve ethanol production from D-lactate, Jen1p, a monocarboxylate-proton symporter, was constitutively expressed in Saccharomyces cerevisiae NAM34-4C. The mutant produced 2.4 g/L of ethanol, approximately 2.4 times higher than that of the wild-type strain. A monocarboxylate/proton symporter gene (JEN1) null mutant was also constructed. It produced 0.19 g/L of ethanol, 5 times lower than that of the wild-type strain.
authors: Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo University, Ikeda, Nishi-ku, Kumamoto, Japan.
authors: Makoto
authors: M
authors: Wakamatsu
authors: Masataka
authors: M
authors: Tomitaka
authors: Tatsunori
authors: T
authors: Tani
authors: Hisataka
authors: H
authors: Taguchi
authors: Kenji
authors: K
authors: Kida
authors: Takashi
authors: T
authors: Akamatsu
mesh_terms: D000431:Ethanol / Q000378:metabolism*; D015870:Gene Expression; D005818:Genetic Engineering / Q000379:methods*; D019344:Lactic Acid / Q000378:metabolism*; D027501:Monocarboxylic Acid Transporters / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D027981:Symporters / Q000235:genetics*
chemical_list: C118697:JEN1 protein, S cerevisiae; D027501:Monocarboxylic Acid Transporters; D029701:Saccharomyces cerevisiae Proteins; D027981:Symporters; D019344:Lactic Acid; D000431:Ethanol