title: Genomic organization and expression analysis of a farnesyl diphosphate synthase gene (FPPS2) in apples (Malus domestica Borkh.).
abstract: A farnesyl diphosphate synthase gene (FPPS2), which contains 11 introns and 12 exons, was isolated from the apple cultivar "White Winter Pearmain". When it was compared to our previously reported FPPS1, its each intron size was different, its each exon size was the same as that of FPPS1 gene, 30 nucleotide differences were found in its coding sequence. Based on these nucleotide differences, specific primers were designed to perform expression analysis; the results showed that it expressed in both fruit and leaf, its expression level was obviously lower than that of FPPS1 gene in fruit which was stored at 4°C for 5 weeks. This is the first report concerning two FPPS genes and their expression comparison in apples.
authors: Shandong Institute of Pomology, Taian 271000, China. yuankj@vip.sina.com
authors: Kejun
authors: K
authors: Yuan
authors: Changjun
authors: C
authors: Wang
authors: Li
authors: L
authors: Xin
authors: Anning
authors: A
authors: Zhang
authors: Chengxiang
authors: C
authors: Ai
mesh_terms: D020816:Amino Acid Motifs; D017931:DNA Primers; D018744:DNA, Plant / Q000032:analysis / Q000235:genetics; D005091:Exons; D005638:Fruit / Q000201:enzymology / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D051228:Geranyltranstransferase / Q000235:genetics*; D007438:Introns; D027845:Malus / Q000201:enzymology / Q000235:genetics*; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D018744:DNA, Plant; D010940:Plant Proteins; D051228:Geranyltranstransferase