title: Physicochemical and biological evaluation of poly(ethylene glycol) methacrylate grafted onto poly(dimethyl siloxane) surfaces for prosthetic devices.
abstract: Poly(dimethyl siloxane) (PDMS) was surface-polymerized with poly(ethylene glycol)methacrylate (PEGMA) by surface-initiated atom transfer radical polymerization (SI-ATRP) in aqueous media at room temperature. Modification of the PDMS surface followed a three-step procedure: (i) PDMS surface hydroxylation by UV/ozone exposure, immediately followed by (ii) covalent attachment of the initiator, 1-trichlorosilyl-2-(chloromethylphenyl)ethane, onto the hydroxylated PDMS, via chemical vapor deposition; finally (iii) PDMS surface-polymerization of PEGMA by ATRP. Modified PDMS was characterized by water contact angle measurement, SEM, FTIR-ATR, and XPS. Results showed that modified surfaces had a hydrophilic character, given the water contact angles around 60°; FTIR-ATR and XPS analysis confirmed the presence of polymerized PEGMA on the surface of PDMS and the adhesion of Staphylococcus aureus GB 2/1 and Streptococcus salivarius GB 24/9 onto the modified surfaces was inhibited 94% and 81%, respectively. Finally, the modified PDMS showed no evidence of cytotoxic effects in in vitro assays using human skin fibroblasts.
authors: IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Braga, Portugal.
authors: Sara
authors: S
authors: Gonçalves
authors: Ana
authors: A
authors: Leirós
authors: Theo
authors: T
authors: van Kooten
authors: Fernando
authors: F
authors: Dourado
authors: Lígia R
authors: LR
authors: Rodrigues
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D002627:Chemistry, Physical; D004129:Dimethylpolysiloxanes / Q000737:chemistry* / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D008826:Microbial Sensitivity Tests; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000494:pharmacology*; D058105:Polymerization; D019736:Prostheses and Implants; D012867:Skin / Q000166:cytology; D013211:Staphylococcus aureus / Q000187:drug effects; D013291:Streptococcus / Q000187:drug effects; D013329:Structure-Activity Relationship; D013499:Surface Properties
chemical_list: D000900:Anti-Bacterial Agents; D004129:Dimethylpolysiloxanes; C450003:poly(ethylene glycol) methacrylate polymer; D011092:Polyethylene Glycols; C013830:baysilon