title: Modulation of the release of Ang-2 in experimental endotoxic shock by a species-specific circulating factor.
abstract: OBJECTIVE
  To study the modulation of the release of angiopoietin-2 (Ang-2) in experimental endotoxic shock.
  
  
  METHODS
  Twelve pigs were studied; eight became septic after the intravenous infusion of lipopolysaccharide (LPS) of Escherichia coli O55:B5. The concentrations of LPS, angiopoietin-2 (Ang-2), tumour necrosis factor-alpha (TNFα) and malondialdehyde (MDA) were measured soon after the LPS infusion in the serum samples from the pulmonary and systemic circulation. Peripheral blood mononuclear cells (PBMCs) were isolated from two healthy swine, from two healthy human donors and from four patients with septic shock. The PBMCs were cultured with the serum of the septic animals in the presence or absence of polymyxin B. Concentrations of Ang-2 and TNFα were measured in supernatants.
  
  
  RESULTS
  Serum Ang-2 was higher in the systemic circulation than in the pulmonary circulation. Increased Ang-2 release was noted in swine PBMCs in the presence of polymyxin B. A reciprocal decrease in TNFα release was observed, typically after incubation with serum sampled from the pulmonary circulation.
  
  
  CONCLUSIONS
  There is evidence for a circulating factor that primes Ang-2 release from blood monocytes in the event of septic shock. The finding indicates a possible site of interference within the septic shock cascade.
authors: 4th Department of Internal Medicine, University of Athens, Medical School, Greece.
authors: Dionyssia-Pinelopi
authors: DP
authors: Carrer
authors: Katerina
authors: K
authors: Kotzampassi
authors: Barbara
authors: B
authors: Fyntanidou
authors: Vasilis
authors: V
authors: Grosomanidis
authors: Andreas
authors: A
authors: Papapetropoulos
authors: Maria
authors: M
authors: Lymperi
authors: Evangelos J
authors: EJ
authors: Giamarellos-Bourboulis
mesh_terms: D042702:Angiopoietin-2 / Q000097:blood* / Q000276:immunology* / Q000627:therapeutic use*; D000818:Animals; D004195:Disease Models, Animal; D006801:Humans; D008297:Male; D009000:Monocytes / Q000276:immunology; D012772:Shock, Septic / Q000503:physiopathology; D013552:Swine; D014409:Tumor Necrosis Factor-alpha / Q000097:blood / Q000276:immunology
chemical_list: D042702:Angiopoietin-2; D014409:Tumor Necrosis Factor-alpha