title: Lutein recovery from Chlorella sp. ESP-6 with coagulants.
abstract: Production of algal lutein included cell cultivation, biomass harvesting, cell wall disruption, and subsequent purification if needed. This work cultivated Chlorella sp. ESP-6 cells in photobioreactor to a biomass content of 1.1 gl(-1) and then the freezing-grinding, ultrasonic treatment (20 and 42kHz) and microwave treatment were used to disrupt the cell walls for recover intracellular lutein. The grinding recovered more lutein than ultrasound or microwave pretreatment. Single coagulation using >30 mgl(-1) chitosan or dual-conditioning using 10 mg l(-1) polyaluminum chloride and 10 mgl(-1) chitosan effectively enhance sedimentation and membrane filtration efficiency of algal suspensions. However, the presence of coagulants lowers the lutein yield from algal biomass in the subsequent 20 kHz ultrasound treatment and purification process. Simulation results revealed affine adsorption of lutein onto chitosan molecules via hydroxyl-amine interaction. The possible drawback by pre-treatment stage should be considered together with the subsequent recovery stage in whole process assessment.
authors: Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 106, Taiwan.
authors: Rhesa Pramudita
authors: RP
authors: Utomo
authors: Yin-Ru
authors: YR
authors: Chang
authors: Duu-Jong
authors: DJ
authors: Lee
authors: Jo-Shu
authors: JS
authors: Chang
mesh_terms: D000536:Aluminum Hydroxide / Q000494:pharmacology*; D018533:Biomass; D048271:Chitosan / Q000494:pharmacology*; D002708:Chlorella / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D005427:Flocculation / Q000187:drug effects; D014975:Lutein / Q000302:isolation & purification*; D008872:Microwaves; D009391:Nephelometry and Turbidimetry; D055672:Static Electricity; D014465:Ultrasonics
chemical_list: C016213:aluminum oxychloride; D000536:Aluminum Hydroxide; D048271:Chitosan; D014975:Lutein