title: Electrochemical disinfection of fish pathogens in seawater without the production of a lethal concentration of chlorine using a flow reactor.
abstract: An electrochemical disinfection system employing a honeycombed platinum coated titanium electrode was developed for the disinfection of seawater. Cell suspensions (2 l, 10³ cells/ml) of the fish pathogens, Vibrio alginolyticus, Edwardsiella tarda, Lactococcus garvieae and Vibrio anguillarum were circulated in a reactor equipped with 10 sets of these electrodes at a flow rate of 200 ml/min with an applied potential of 1.0 V vs. Ag/AgCl reference electrode. The circulated cells were completely disinfected after 3 h of treatment, whereas free residual chlorine generated due to seawater electrolysis was below 0.1 ppm. In addition, a diphenyl-1-pyrenylphosphine fluorescent assay revealed that lipid peroxidation in the cell membranes of disinfected bacteria was induced probably by reactive oxygen species generated during electrochemical treatment.
authors: Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan. tsuyo@cc.tuat.ac.jp
authors: Tsuyoshi
authors: T
authors: Tanaka
authors: Mari
authors: M
authors: Shimoda
authors: Nozomi
authors: N
authors: Shionoiri
authors: Masahito
authors: M
authors: Hosokawa
authors: Tomoyuki
authors: T
authors: Taguchi
authors: Hitoshi
authors: H
authors: Wake
authors: Tadashi
authors: T
authors: Matsunaga
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000473:pathology; D002713:Chlorine / Q000032:analysis* / Q000633:toxicity; D004203:Disinfection / Q000295:instrumentation* / Q000379:methods*; D004566:Electrodes; D004572:Electrolysis / Q000295:instrumentation* / Q000379:methods*; D005399:Fishes / Q000382:microbiology*; D005456:Fluorescent Dyes / Q000032:analysis; D015227:Lipid Peroxidation; D009943:Organophosphorus Compounds / Q000032:analysis; D011721:Pyrenes / Q000032:analysis; D017382:Reactive Oxygen Species / Q000378:metabolism; D012623:Seawater / Q000382:microbiology*; D013997:Time Factors; D014025:Titanium
chemical_list: D005456:Fluorescent Dyes; D009943:Organophosphorus Compounds; D011721:Pyrenes; D017382:Reactive Oxygen Species; C066322:diphenyl-1-pyrenylphosphine; D002713:Chlorine; D014025:Titanium
keywords: Disinfection byproducts; Electrochemical disinfection; Flow reactor; Lipid peroxidation; Marine pathogen