title: Micro-particle image velocimetry for velocity profile measurements of micro blood flows.
abstract: Micro-particle image velocimetry (μPIV) is used to visualize paired images of micro particles seeded in blood flows. The images are cross-correlated to give an accurate velocity profile. A protocol is presented for μPIV measurements of blood flows in microchannels. At the scale of the microcirculation, blood cannot be considered a homogeneous fluid, as it is a suspension of flexible particles suspended in plasma, a Newtonian fluid. Shear rate, maximum velocity, velocity profile shape, and flow rate can be derived from these measurements. Several key parameters such as focal depth, particle concentration, and system compliance, are presented in order to ensure accurate, useful data along with examples and representative results for various hematocrits and flow conditions.
authors: Department of Chemical and Biological Engineering, University of Ottawa.
authors: Katie L
authors: KL
authors: Pitts
authors: Marianne
authors: M
authors: Fenech
mesh_terms: D000818:Animals; D056656:Lab-On-A-Chip Devices; D010316:Particle Size; D012039:Regional Blood Flow / Q000502:physiology*; D012212:Rheology / Q000295:instrumentation / Q000379:methods*; D013552:Swine
references: Annu Rev Fluid Mech. 2005 Jan 1;37:43-69
references: 21151769
references: Ann Biomed Eng. 2004 Jun;32(6):803-14
references: 15255211
references: Physiol Meas. 2012 Oct;33(10):1585-602
references: 22945542
references: Electrophoresis. 2003 Nov;24(21):3563-76
references: 14613181
references: J Physiol. 2011 Mar 1;589(Pt 5):1047-52
references: 21242255
references: Physiol Rev. 2011 Jan;91(1):327-87
references: 21248169
references: J Mech Behav Biomed Mater. 2013 Jan;17:333-6
references: 23127640
references: Angew Chem Int Ed Engl. 1998 Mar 16;37(5):550-575
references: 29711088
references: Transfusion. 1999 Mar;39(3):277-81
references: 10204590
references: Electrophoresis. 2010 Jan;31(1):2-16
references: 20039289