title: [Effects of pioglitazone on TGFbeta1 expression in ischemia/reperfusion injury myocardium of rats].
abstract: OBJECTIVE
  To investigate the effects of pioglitazone on transforming growth factor beta1 (TGFbeta1) expression in ischemia/reperfusion injury myocardium of rats.
  
  
  METHODS
  Thirty SD rats were randomly divided into five groups (n = 6): ischemia/reperfusion group, pioglitazone 5 mg/(kg x d) group, pioglitazone 10 mg/(kg x d) group, pioglitazone 20 mg/(kg x d) group and pioglitazone 20 mg/(kg x d) + peroxisome proliferator-activated receptor gamma (PPARgamma) specific antagonist GW9662 group. Left anterior descending coronary artery of rats were ligated for 30 min and reperfused for 120 min to establish the model of ischemia/reperfusion in vivo. RT-PCR was performed to detect the expression of TGFbeta1 mRNA. Western blot was performed to detect the expression of TGFbeta1 protein.
  
  
  RESULTS
  Myocardial apoptosis was significantly suppressed by pioglitazone. Pioglitazone upregulated TGFPbeta1 expression both in mRNA and protein level. GW9662 reversed the inhibition of myocardial apoptosis and the upregulation of TGFbeta1 expression by pioglitazone.
  
  
  CONCLUSIONS
  Pioglitazone can inhibit the myocardial apoptosis induced by ischemia/reperfusion. Pioglitazone may protect the myocardium from ischemia/reperfusion via upregulation of TGFbeta1. This protection may be mediated by PPARgamma.
authors: Department of Geriatric Cardiology, Chinese PLA General Hospital, Beijing 100853, China. wanghao2003@sina.com
authors: Hao
authors: H
authors: Wang
authors: Ping
authors: P
authors: Ye
authors: Yang
authors: Y
authors: Li
authors: Zong-Bin
authors: ZB
authors: Li
authors: Lin
authors: L
authors: Wang
mesh_terms: D000813:Anilides / Q000494:pharmacology; D000818:Animals; D017209:Apoptosis; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D047495:PPAR gamma / Q000037:antagonists & inhibitors; D000077205:Pioglitazone; D051381:Rats; D017207:Rats, Sprague-Dawley; D045162:Thiazolidinediones / Q000494:pharmacology*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
chemical_list: C457499:2-chloro-5-nitrobenzanilide; D000813:Anilides; D047495:PPAR gamma; C508962:Tgfb1 protein, rat; D045162:Thiazolidinediones; D053773:Transforming Growth Factor beta1; D000077205:Pioglitazone