title: Arf1 and membrane curvature cooperate to recruit Arfaptin2 to liposomes.
abstract: Arfaptin2 contains a Bin/Amphiphysin/Rvs (BAR) domain and directly interacts with proteins of the Arf/Arl family in their active GTP-bound state. It has been proposed that BAR domains are able to sense membrane curvature and to induce membrane tubulation. We report here that active Arf1 is required for the recruitment of Arfaptin2 to artificial liposomes mimicking the Golgi apparatus lipid composition. The Arf1-dependent recruitment of Arfaptin2 increases with membrane curvature, while the recruitment of Arf1 itself is not sensitive to curvature. At high protein concentrations, the binding of Arfaptin2 induces membrane tubulation. Finally, membrane-bound Arfaptin2 is released from the liposome when ArfGAP1 catalyzes the hydrolysis of GTP to GDP in Arf1. These results show that both Arf1 activation and high membrane curvature are required for efficient recruitment of Arfaptin2 to membranes.
authors: Laboratorio de Biofísica de Biomembranas. Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC, UNC-CONICET), Departamento de Química Biológica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina. ambroggioernesto@yahoo.com
authors: Ernesto E
authors: EE
authors: Ambroggio
authors: James
authors: J
authors: Sillibourne
authors: Bruno
authors: B
authors: Antonny
authors: Jean-Baptiste
authors: JB
authors: Manneville
authors: Bruno
authors: B
authors: Goud
mesh_terms: D020823:ADP-Ribosylation Factor 1 / Q000378:metabolism*; D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D006160:Guanosine Triphosphate / Q000378:metabolism; D006801:Humans; D008081:Liposomes / Q000378:metabolism*; D021381:Protein Transport; D013379:Substrate Specificity; D053835:Unilamellar Liposomes / Q000378:metabolism
chemical_list: C101158:ARFIP2 protein, human; D048868:Adaptor Proteins, Signal Transducing; D008081:Liposomes; D053835:Unilamellar Liposomes; D006160:Guanosine Triphosphate; D020823:ADP-Ribosylation Factor 1
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