title: CD4+ T-cell responses among adults and young children in response to Streptococcus pneumoniae and Haemophilus influenzae vaccine candidate protein antigens.
abstract: We characterized cytokine profiles of CD4(+) T-helper (h) cells in adults and young children to ascertain if responses occur to next-generation candidate vaccine antigens PspA, PcpA, PhtD, PhtE, Ply, LytB of Streptococcus pneumonia (Spn) and protein D and OMP26 of non-typeable Haemophilus influenzae (NTHi). Adults had vaccine antigen-specific Th1 and Th2 cells responsive to all antigens evaluated whereas young children had significant numbers of vaccine antigen-specific CD4(+) T cells producing IL-2, (p=0.004). Vaccine antigen-specific CD4(+) T-cell populations in adults were largely of effector (TEM) and/or central memory (TCM) phenotypes as defined by CD45RA(-)CCR7(+) or CD45RA(-)CCR7(-) respectively; however among young children antigen-specific IL-2 producing CD4(+) T cells demonstrated CD45RA(+) expression (non-memory cells). We conclude that adults have circulating memory CD4(+) T cells (CD45RA(-)) that can be stimulated by all the tested Spn and NTHi protein vaccine candidate antigens, whereas young children have a more limited response.
authors: Center for Infectious Disease and Immunology, Research Institute, Rochester General Hospital, 1425 Portland Avenue, Rochester, NY 14621, USA.
authors: Sharad K
authors: SK
authors: Sharma
authors: David
authors: D
authors: Roumanes
authors: Anthony
authors: A
authors: Almudevar
authors: Tim R
authors: TR
authors: Mosmann
authors: Michael E
authors: ME
authors: Pichichero
mesh_terms: D000328:Adult; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000008:administration & dosage / Q000276:immunology; D001426:Bacterial Proteins / Q000008:administration & dosage / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D002352:Carrier Proteins / Q000008:administration & dosage / Q000276:immunology; D002675:Child, Preschool; D016207:Cytokines / Q000276:immunology; D005260:Female; D006192:Haemophilus Infections / Q000517:prevention & control*; D018073:Haemophilus Vaccines / Q000008:administration & dosage / Q000276:immunology*; D006801:Humans; D007072:Immunoglobulin D / Q000008:administration & dosage / Q000276:immunology; D007223:Infant; D007376:Interleukin-2 / Q000276:immunology; D008074:Lipoproteins / Q000008:administration & dosage / Q000276:immunology; D008297:Male; D011008:Pneumococcal Infections / Q000517:prevention & control*; D022242:Pneumococcal Vaccines / Q000008:administration & dosage / Q000276:immunology*; D018272:Porins / Q000276:immunology; D013296:Streptococcus pneumoniae / Q000276:immunology*; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D016207:Cytokines; D018073:Haemophilus Vaccines; D007072:Immunoglobulin D; D007376:Interleukin-2; D008074:Lipoproteins; C118414:OMP26 protein, Haemophilus influenzae; D022242:Pneumococcal Vaccines; D018272:Porins; C451502:ompP2 protein, Haemophilus influenzae; C067989:glpQ protein, Haemophilus influenzae
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