title: Prevalence of tuberculosis in pigs slaughtered at two abattoirs in Ethiopia and molecular characterization of Mycobacterium tuberculosis isolated from tuberculous-like lesions in pigs.
abstract: BACKGROUND
  Tuberculosis (TB) is an infectious, granulomatous disease caused by acid-fast bacilli of the genus Mycobacterium. The disease affects practically all species of vertebrates. Although mammalian tuberculosis has been nearly controlled in many developed countries, it is still a serious problem in humans and domestic animals including pigs in developing countries. In Ethiopia, the prevalence of TB in pigs is not known. Therefore, this study was designed to estimate the prevalence of TB in pigs in central Ethiopia and to characterize the causative agents using molecular techniques.
  
  
  RESULTS
  The estimated prevalence of TB was 5.8% (49/841). Age and origin of pigs were significantly associated (P<0.001) with the prevalence. In contrast, an association of sex, floor type and water source with the prevalence could not be shown. Culture positivity was confirmed in 30.6% (15/49) of the tuberculous-like lesions. Of the 15 isolates, 12 were acid fast positive while five of the latter were confirmed by multiplex PCR as members of the M. tuberculosis complex. Speciation of the five isolates further confirmed that they were M. tuberculosis, belonging to SIT1088 (two isolates) and SIT1195 (one isolate). The remaining two isolates belong to an identical spoligotype, the pattern of which was not found in the spoligotype database (SpolDB4).
  
  
  CONCLUSIONS
  The isolation of M. tuberculosis from pigs suggests a possible risk of transmission between humans and pigs. Hence, establishing feasible control methods is required.
authors: Department of Veterinary Clinical Medicine, Faculty of Veterinary Medicine, University of Gondar, PO Box 196, Gondar, Ethiopia. sintumu@yahoo.com
authors: Sintayehu Mulugeta
authors: SM
authors: Arega
authors: Franz Josef
authors: FJ
authors: Conraths
authors: Gobena
authors: G
authors: Ameni
mesh_terms: D000003:Abattoirs / Q000706:statistics & numerical data*; D000822:Animal Husbandry; D000818:Animals; D005002:Ethiopia / Q000453:epidemiology; D005260:Female; D008297:Male; D060885:Multiplex Polymerase Chain Reaction / Q000662:veterinary; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification*; D015995:Prevalence; D012307:Risk Factors; D013552:Swine / Q000382:microbiology; D013553:Swine Diseases / Q000453:epidemiology* / Q000382:microbiology; D014376:Tuberculosis / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*
references: J Clin Microbiol. 2009 Sep;47(9):2794-801
references: 19553569
references: J Clin Microbiol. 1999 May;37(5):1254-9
references: 10203466
references: Vet Rec. 2000 Jun 3;146(23):659-65
references: 10883854
references: J Infect Dis. 2005 Jan 15;191(2):147-9
references: 15609222
references: BMC Microbiol. 2006 Mar 06;6:23
references: 16519816
references: J Clin Microbiol. 1997 Apr;35(4):907-14
references: 9157152
references: Clin Microbiol Rev. 2006 Oct;19(4):658-85
references: 17041139
references: Rev Sci Tech. 2007 Dec;26(3):711-9
references: 18293619
references: Vet Microbiol. 2003 Dec 2;97(1-2):123-33
references: 14637044
references: Clin Infect Dis. 2001 Oct 15;33(8):1363-74
references: 11550115
references: J Vet Med B Infect Dis Vet Public Health. 2002 Nov;49(9):415-8
references: 12489708
references: Acta Vet Hung. 2004;52(2):143-50
references: 15168745
references: Vet J. 2000 Sep;160(2):92-106
references: 10985801
references: Clin Vaccine Immunol. 2007 Oct;14(10):1356-61
references: 17761523
references: Vet Rec. 1984 May 19;114(20):497-500
references: 6740882
references: PLoS One. 2009;4(4):e5068
references: 19352493
references: J Clin Microbiol. 2006 Jan;44(1):29-34
references: 16390943
references: PLoS One. 2011 Jan 24;6(1):e15862
references: 21283668
references: Vet Microbiol. 1994 May;40(1-2):1-4
references: 8073618
references: N Engl J Med. 2008 Mar 13;358(11):1089-92
references: 18337598
references: Trop Anim Health Prod. 2009 Jun;41(5):755-65
references: 19058024
references: Vet Rec. 2010 Jul 24;167(4):138-41
references: 20656993
references: J Vet Diagn Invest. 2009 Jan;21(1):48-52
references: 19139500
references: PCR Methods Appl. 1992 May;1(4):269-73
references: 1282431
references: Appl Environ Microbiol. 1995 Jan;61(1):371-3
references: 7887618
references: Res Vet Sci. 2003 Feb;74(1):1-15
references: 12507561
references: Int J Tuberc Lung Dis. 2001 Dec;5(12):1164-5
references: 11769779
references: Trop Anim Health Prod. 2010 Jun;42(5):905-13
references: 20012691
references: Int J Tuberc Lung Dis. 2010 Oct;14(10):1259-65
references: 20843416
references: J Clin Microbiol. 2005 Mar;43(3):1261-8
references: 15750094