title: Characterization of blood lipoproteins and validation of cholesterol and triacylglycerol assays for free-ranging polar bears (Ursus maritimus).
abstract: Blood triacylglycerol (TG) and lipoproteins are important variables for evaluating nutritional status of wildlife, but measurements are often expensive and difficult. Performance of a small, portable blood analyzer intended for human medical diagnostics was evaluated in measuring these variables in plasma and serum from free-ranging polar bears (Ursus maritimus), which are experiencing nutritional stress related to sea ice loss. The analyzer accurately tracked changes in concentration of total cholesterol (Ctotal), cholesterol associated with high-density lipoprotein (CHDL), and TG during a validation protocol of diluting samples and spiking them with exogenous cholesterol and glycerol. Values of Ctotal and TG agreed well with values obtained by other methods (ultracentrifugation followed by colorimetric assays); agreement was variable for values of cholesterol associated with specific lipoproteins. Similar to a study of captive polar bears, ultracentrifugation methods revealed greater TG in very low-density lipoproteins than in low-density lipoprotein, which is unusual and merits additional study.
authors: Department of Zoology and Physiology, University of Wyoming, 1000 East University Avenue, Laramie, WY, USA. jwhitema@uwyo.edu
authors: John P
authors: JP
authors: Whiteman
authors: Nicholas
authors: N
authors: Frank
authors: Katie A
authors: KA
authors: Greller
authors: Henry J
authors: HJ
authors: Harlow
authors: Merav
authors: M
authors: Ben-David
mesh_terms: D000818:Animals; D002784:Cholesterol / Q000097:blood*; D015203:Reproducibility of Results; D014280:Triglycerides / Q000097:blood*; D001503:Ursidae / Q000097:blood*
chemical_list: D014280:Triglycerides; D002784:Cholesterol
keywords: Cholesterol; lipoproteins; methods; polar bears; triacylglycerol