title: Biotin-conjugated anti-CD44 antibody-avidin binding system for the improvement of chondrocyte adhesion to scaffolds.
abstract: The clinical need for improved treatment options for patients with cartilage injuries has motivated tissue-engineering studies aimed at the in vitro generation of cell-based implants with functional properties. The success of tissue-engineered repair of cartilage may depend on the rapid and efficient adhesion of transplanted cells to the scaffold. In the present study, chondrocyte-scaffold constructs were engineered by planting porcine chondrocytes into nonporous chitosan membranes and 3D porous chitosan scaffolds that were treated with or without biotin-conjugated anti-CD44 antibody-avidin binding system and avidin-biotin binding system. The spreading area, cell exfoliation rates, cell proliferation rates, histological analysis, DNA and glycosaminoglycan (GAG) content, and mRNA expression were investigated to evaluate the efficiency of biotin-conjugated anti-CD44 antibody-avidin binding system for the improvement of cell adhesion to scaffolds in the cartilage tissue. The results showed that the biotin-conjugated anti-CD44 antibody-avidin binding system improved cell adhesion to scaffolds effectively. These studies suggest that this binding system has the potential to provide improved tissue-engineered cartilage for clinical applications.
authors: Department of Orthopaedic Surgery, Zhongshan Hospital, Fudan University, Shanghai, 200032, China.
authors: Hong
authors: H
authors: Lin
authors: Jian
authors: J
authors: Zhou
authors: Longxiang
authors: L
authors: Shen
authors: Yuhui
authors: Y
authors: Ruan
authors: Jian
authors: J
authors: Dong
authors: Changan
authors: C
authors: Guo
authors: Zhengrong
authors: Z
authors: Chen
mesh_terms: D000818:Animals; D000906:Antibodies / Q000378:metabolism*; D001360:Avidin; D001710:Biotin / Q000378:metabolism*; D002356:Cartilage / Q000378:metabolism; D002448:Cell Adhesion; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism*; D004247:DNA / Q000378:metabolism; D005786:Gene Expression Regulation; D006025:Glycosaminoglycans / Q000378:metabolism; D018960:Hyaluronan Receptors / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013194:Staining and Labeling; D034421:Sus scrofa; D013997:Time Factors; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000906:Antibodies; D006025:Glycosaminoglycans; D018960:Hyaluronan Receptors; D012333:RNA, Messenger; D001360:Avidin; D001710:Biotin; D004247:DNA
keywords: anti-CD44 antibody; avidin-biotin; cartilage tissue engineering; cell adhesion; chondrocytes