title: Development, expansion, and in vivo monitoring of human NK cells from human embryonic stem cells (hESCs) and and induced pluripotent stem cells (iPSCs).
abstract: We present a method for deriving natural killer (NK) cells from undifferentiated hESCs and iPSCs using a feeder-free approach. This method gives rise to high levels of NK cells after 4 weeks culture and can undergo further 2-log expansion with artificial antigen presenting cells. hESC- and iPSC-derived NK cells developed in this system have a mature phenotype and function. The production of large numbers of genetically modifiable NK cells is applicable for both basic mechanistic as well as anti-tumor studies. Expression of firefly luciferase in hESC-derived NK cells allows a non-invasive approach to follow NK cell engraftment, distribution, and function. We also describe a dual-imaging scheme that allows separate monitoring of two different cell populations to more distinctly characterize their interactions in vivo. This method of derivation, expansion, and dual in vivo imaging provides a reliable approach for producing NK cells and their evaluation which is necessary to improve current NK cell adoptive therapies.
authors: Department of Medicine Hematology, Oncology, and Transplant, University of Minnesota, Minneapolis, USA.
authors: Allison M
authors: AM
authors: Bock
authors: David
authors: D
authors: Knorr
authors: Dan S
authors: DS
authors: Kaufman
mesh_terms: D002454:Cell Differentiation / Q000502:physiology; D003584:Cytological Techniques / Q000379:methods*; D053595:Embryonic Stem Cells / Q000166:cytology*; D006801:Humans; D007694:Killer Cells, Natural / Q000166:cytology*; D039904:Pluripotent Stem Cells / Q000166:cytology*
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