title: [Effect of recombinant human growth hormone on the secretion of TNF-α and IL-6 by THP-1 cells and its correlation with the NF-κB pathway].
abstract: OBJECTIVE
  To investigate the effect of recombinant human growth hormone (rhGH) on the secretion of TNF-α and IL-6 in THP-1 cells and analyze the correlation between the secretion and NF-κB signaling pathway.
  
  
  METHODS
  The concentrations of TNF-α and IL-6 in the supernatants of THP-1 induced by rhGH were measured with ELISA. The effects of LPS and an NF-κB inhibitor, BAY11-7082 on the secretion of TNF-α and IL-6 were also observed. The activity of NF-κB was detected by luciferase reporter and electrophoretic mobility shift assay (EMSA) in THP-1 cells induced by rhGH.
  
  
  RESULTS
  rhGH alone promoted the secretion of TNF-α and IL-6 in THP-1 cells while inhibited the secretion of TNF-α and IL-6 in THP-1 stimulated by LPS. BAY11-7082 inhibited the secretion of TNF-α and IL-6 in THP-1 stimulated by LPS and rhGH. The activity of NF-κB was significantly correlated with the secretion of TNF-α and IL-6.
  
  
  CONCLUSIONS
  rhGH played a bidirectional role in the regulation on the secretion of TNF-α and IL-6 via NF-κB signaling pathway in THP-1 cells.
authors: Nursing Institute, Xinxiang Medical University, China. zrq19221@163.com
authors: Ruiqin
authors: R
authors: Zhang
authors: Jing
authors: J
authors: Tan
authors: Guojun
authors: G
authors: Zhang
authors: Ruling
authors: R
authors: Shi
authors: Xiangfeng
authors: X
authors: Song
authors: Lanzhi
authors: L
authors: Mao
mesh_terms: D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D019382:Human Growth Hormone / Q000494:pharmacology*; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism*; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
chemical_list: D015850:Interleukin-6; D016328:NF-kappa B; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D019382:Human Growth Hormone