title: Phytochemical investigation and pharmacognostic standardization of Cissampelos pareira root.
abstract: BACKGROUND
  In ethno-medicinal practices, the roots of Cissampelos pareira(Patha) are used in the treatment of various ailments related to urinary problems and skin infections, and in tumorinhibitor activity, antibacterial, antimalarial, diuretic activity,anticonvulsant activity etc., The main problem encountered in standardization of Ayurvedic drugs is proper identification of the source plant.
  
  
  METHODS
  The macroscopic features of each anatomical component have been observed by a high-resolution camera. The moisture content, total ash, acid-insoluble ash, and water-alcohol and ether extractive values of the powdered sampleswere determined by the method as per WHO guidelines. The phytochemical investigation of the methanolic extract of Cissampelos pareira root was performed by the standard chemical ltests and by TLC on silica gel G using solvent systems proposed by Harborne.
  
  
  RESULTS
  The microscopic characteristics showed the wavy epidermis with unicellular trichomes. Lignified xylem vessels, radial medullary rays, and prismatic calcium oxalate crystals had also been found. Phytochemical screening revealed that the Cissampelos pareira root extract contains terpenoids, alkaloids, tannins, amino acid proteins, and carbohydrates. Alkaloids and essential oil were detected in TLC of the Cissampelos pareira root extract developed using blends of methanol:concentrated ammonia (200:3) and n-butanol:acetone:water (3:1:1) and benzene:ethyl acetate:formic acid(9:7:4) as solvent systems for alkaloid whereas chloroform(100%), benzene (100%), chloroform:benzene(1:1),and ether:benzene(1:1) as solvent systems for essential oil.
  
  
  CONCLUSIONS
  These findings will be useful toward establishing pharmacognostic standards on identification, purity,quality, and classification of the plant, which is gaining relevance in plant drug research.
authors: Rayat and Bahra Institute of Pharmacy, Sahauran, Kharar, Mohali, Punjab, India.
authors: Jhuma
authors: J
authors: Samanta
authors: S
authors: S
authors: Bhattacharya
authors: Rana
authors: R
authors: Rayat
keywords: Cissampelos pareira; pharmacognostic standards; pharmacognostical and phytochemical investigation
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