title: GmTMT2a from soybean elevates the α-tocopherol content in corn and Arabidopsis.
abstract: Tocochromanol, or vitamin E, plays a crucial role in human and animal nutrition and is synthesized only by photosynthetic organisms. γ-Tocopherol methyltransferase (γ-TMT), one of the key enzymes in the tocopherol biosynthetic pathway in plants, converts γ, δ-tocopherols into α-, β-tocopherols. Tocopherol content was investigated in 15 soybean cultivars and GmTMT2 was isolated from five varieties based on tocopherol content. GmTMT2a was expressed in E. coli and the purified protein effectively converted γ-tocopherol into α-tocopherol in vitro. Overexpression of GmTMT2a enhanced α-tocopherol content 4-6-fold in transgenic Arabidopsis, and α-tocopherol content increased 3-4.5-fold in transgenic maize seed, which correlated with the accumulation of GmTMT2a. Transgenic corn that is α-tocopherol-rich may be beneficial for animal health and growth.
authors: Biotechnology Research Institute, National Key Facility of Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors: Lan
authors: L
authors: Zhang
authors: Yanzhong
authors: Y
authors: Luo
authors: Yongxing
authors: Y
authors: Zhu
authors: Liang
authors: L
authors: Zhang
authors: Wei
authors: W
authors: Zhang
authors: Rumei
authors: R
authors: Chen
authors: Miaoyun
authors: M
authors: Xu
authors: Yunliu
authors: Y
authors: Fan
authors: Lei
authors: L
authors: Wang
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D017931:DNA Primers / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli; D008780:Methyltransferases / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D013025:Glycine max / Q000235:genetics*; D003313:Zea mays / Q000235:genetics / Q000378:metabolism*; D024502:alpha-Tocopherol / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D008780:Methyltransferases; C047259:gamma-tocopherol methyltransferase; D024502:alpha-Tocopherol
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