title: The DEAD-box helicase DDX3 substitutes for the cap-binding protein eIF4E to promote compartmentalized translation initiation of the HIV-1 genomic RNA.
abstract: Here, we show a novel molecular mechanism promoted by the DEAD-box RNA helicase DDX3 for translation of the HIV-1 genomic RNA. This occurs through the adenosine triphosphate-dependent formation of a translation initiation complex that is assembled at the 5' m(7)GTP cap of the HIV-1 mRNA. This is due to the property of DDX3 to substitute for the initiation factor eIF4E in the binding of the HIV-1 m(7)GTP 5' cap structure where it nucleates the formation of a core DDX3/PABP/eIF4G trimeric complex on the HIV-1 genomic RNA. By using RNA fluorescence in situ hybridization coupled to indirect immunofluorescence, we further show that this viral ribonucleoprotein complex is addressed to compartmentalized cytoplasmic foci where the translation initiation complex is assembled.
authors: CIRI, International Center for Infectiology Research, Université de Lyon, Lyon 69634, France. rsotorifo@med.uchile.cl
authors: Ricardo
authors: R
authors: Soto-Rifo
authors: Paulina S
authors: PS
authors: Rubilar
authors: Théophile
authors: T
authors: Ohlmann
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D002451:Cell Compartmentation; D003594:Cytoplasmic Granules / Q000737:chemistry; D053487:DEAD-box RNA Helicases / Q000032:analysis / Q000502:physiology*; D039561:Eukaryotic Initiation Factor-4E / Q000378:metabolism; D016679:Genome, Viral; D015497:HIV-1 / Q000235:genetics* / Q000502:physiology; D006367:HeLa Cells; D006801:Humans; D019169:Jurkat Cells; D010442:Peptide Chain Initiation, Translational; D012315:RNA Caps / Q000378:metabolism; D012367:RNA, Viral / Q000032:analysis / Q000096:biosynthesis*; D014779:Virus Replication
chemical_list: D039561:Eukaryotic Initiation Factor-4E; D012315:RNA Caps; D012367:RNA, Viral; D000255:Adenosine Triphosphate; C119332:DDX3X protein, human; D053487:DEAD-box RNA Helicases
references: PLoS One. 2009 Nov 24;4(11):e7997
references: 19956697
references: Adv Virus Res. 2009;74:1-40
references: 19698894
references: J Biol Chem. 2012 May 25;287(22):18500-9
references: 22493286
references: Proc Natl Acad Sci U S A. 1997 May 13;94(10):5201-6
references: 9144215
references: Cell. 2009 Feb 20;136(4):688-700
references: 19239889
references: EMBO J. 1988 Sep;7(9):2831-7
references: 3181141
references: Nat Rev Mol Cell Biol. 2011 Jul 22;12(8):505-16
references: 21779027
references: Cell. 2004 Oct 29;119(3):381-92
references: 15507209
references: Traffic. 2006 Nov;7(11):1440-50
references: 16984406
references: Cell. 2006 Dec 29;127(7):1389-400
references: 17190602
references: Trends Biochem Sci. 2003 Apr;28(4):215-20
references: 12713906
references: Nucleic Acids Res. 2007;35(18):e121
references: 17881372
references: Microbiol Mol Biol Rev. 2000 Jun;64(2):239-80
references: 10839817
references: Science. 2005 Sep 2;309(5740):1514-8
references: 16141059
references: Cell. 2009 Feb 20;136(4):719-30
references: 19239891
references: PLoS Pathog. 2009 May;5(5):e1000437
references: 19478882
references: Biochemistry. 1999 Jun 29;38(26):8538-47
references: 10387101
references: J Mol Biol. 2002 Jun 7;319(3):615-35
references: 12054859
references: PLoS Pathog. 2010 Nov 18;6(11):e1001200
references: 21124996
references: Proc Natl Acad Sci U S A. 2010 Aug 17;107(33):14787-92
references: 20679221
references: Annu Rev Microbiol. 1998;52:491-532
references: 9891806
references: J Biol Chem. 2009 Nov 6;284(45):31350-62
references: 19737937
references: Retrovirology. 2008 Oct 28;5:97
references: 18957126
references: Cell. 1994 Aug 26;78(4):657-68
references: 8069914
references: RNA. 2010 Jan;16(1):221-7
references: 19933321
references: J Biol Chem. 2011 Jul 1;286(26):22758-68
references: 21536667
references: Nature. 1986 May 22-28;321(6068):412-7
references: 3012355
references: J Mol Biol. 2007 May 4;368(3):691-705
references: 17368478
references: EMBO J. 2012 Sep 12;31(18):3745-56
references: 22872150
references: J Biol Chem. 2001 Apr 20;276(16):12959-66
references: 11278368
references: Cell. 2001 Sep 7;106(5):607-17
references: 11551508
references: Cell. 2009 Feb 20;136(4):731-45
references: 19239892
references: Nucleic Acids Res. 1988 Sep 26;16(18):8953-62
references: 3174438
references: Cell. 2010 Aug 6;142(3):368-74
references: 20691898
references: Virus Res. 2012 Nov;169(2):430-7
references: 22705970
references: J Cell Sci. 2010 Feb 1;123(Pt 3):369-83
references: 20053637
references: J Virol. 1986 Aug;59(2):284-91
references: 3016298
references: Mol Cell. 2011 Sep 16;43(6):962-72
references: 21925384
references: Retrovirology. 2004 Dec 08;1:42
references: 15588285
references: Nucleic Acids Res. 2011 Jul;39(12):5232-44
references: 21393254
references: Biochem J. 2012 Jan 1;441(1):119-29
references: 21883093
references: Mol Cell. 2002 Sep;10(3):585-97
references: 12408826
references: Mol Biol Cell. 2008 Sep;19(9):3847-58
references: 18596238
references: Cell. 1997 Sep 19;90(6):1051-60
references: 9323133
references: J Mol Biol. 2012 Jan 6;415(1):61-74
references: 22051512
references: Nucleic Acids Res. 2012 Mar;40(6):2653-67
references: 22121214
references: Trends Biochem Sci. 2006 Dec;31(12):687-93
references: 17084632
references: J Biol Chem. 2006 Oct 27;281(43):32870-8
references: 16951406
references: PLoS Pathog. 2012;8(3):e1002612
references: 22457629
references: Genes Dev. 2009 Nov 1;23(21):2537-50
references: 19884259
references: Retrovirology. 2006 Mar 17;3:18
references: 16545126
references: Trends Biochem Sci. 2008 Mar;33(3):141-50
references: 18291657
references: J Virol. 1994 Sep;68(9):5433-8
references: 8057425
references: Nucleic Acids Res. 2008 Aug;36(14):4708-18
references: 18628297
references: Methods Enzymol. 2007;431:61-81
references: 17923231
references: Nat Struct Mol Biol. 2009 Sep;16(9):930-7
references: 19668212
references: Gene. 2006 Feb 15;367:17-37
references: 16337753
references: Mol Biol Cell. 2009 Jun;20(11):2673-83
references: 19369421
references: FEBS J. 2012 Sep;279(17):3098-111
references: 22759308
references: Wiley Interdiscip Rev RNA. 2011 Mar-Apr;2(2):277-98
references: 21957010
references: Mol Cell Proteomics. 2012 Apr;11(4):M111.015313
references: 22174317
references: Oncogene. 2008 Jan 24;27(5):700-14
references: 17667941
references: RNA. 2005 Sep;11(9):1355-63
references: 16043498
references: PLoS One. 2009 Sep 14;4(9):e7030
references: 19750007
references: Biochem Soc Trans. 2008 Aug;36(Pt 4):690-3
references: 18631141
references: Retrovirology. 2010 May 10;7:41
references: 20459747