title: [Local adenosine A1 receptors of baihui acupoint mediate cerebral ischemia tolerance induced by Electroacupuncture].
abstract: OBJECTIVE
  To investigate whether local A1R of Baihui acupoint mediate cerebral ischemia tolerance induced by electro-acupuncture (EA).
  
  
  METHODS
  Sixty SD rats were randomly divided into five groups, i.e., the sham-operation (S) group, the model group (M), the electroacupuncture (E) group, the CCPA group and the DMSO group. The focal cerebral ischemia/reperfusion model was established by middle cerebral artery occlusion (MCAO) in rats. Rats in the E group were received EA pretreatment baihui acupoint at 2 h before established MCAO. The rats in DMSO group and the CCPA group were injected with DMSO (20 µl) and CCPA (0.1 mmol/L) 20 µl into Baihui, respectively, at 2 h before established MCAO. After 24 h reperfusion, the rats' behavior, cerebral infarct volume, the cerebral Bcl-2 protein expression were assessed.
  
  
  RESULTS
  Compared with M group, the rats' behavior was improved, the cerebral infarct volume was decreased and the Bcl-2 protein expression was up-regulated (P < 0.05) in the E group. Compared with M and DMSO group, the rats' behavior was improved, the cerebral infarct volume was decreased and the Bcl-2 protein expression was up-regulated (P < 0.05) in the CCPA group. There were no statistical differences between CCPA and E group.
  
  
  CONCLUSIONS
  EA induced cerebral ischemia tolerance. Local A1R of Baihui acupoint possible mediate cerebral ischemia tolerance induced by Electroacupuncture.
authors: Department of Anesthiology, First Affiliated Hospital of Wenzhou Medical College, China.
authors: Dong-dong
authors: DD
authors: Liang
authors: Hong-fa
authors: HF
authors: Wang
authors: Ming-xiao
authors: MX
authors: Zhang
authors: Qin-xue
authors: QX
authors: Dai
authors: Hai-peng
authors: HP
authors: Liu
authors: Yun-chang
authors: YC
authors: Mo
authors: Jun-lu
authors: JL
authors: Wang
mesh_terms: D015669:Acupuncture Points; D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism* / Q000628:therapy*; D015671:Electroacupuncture; D019194:Ischemic Preconditioning / Q000379:methods; D008297:Male; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D043682:Receptor, Adenosine A1 / Q000378:metabolism*
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D043682:Receptor, Adenosine A1