title: Comparison of in-house developed ELISA with HPLC for measurement of hba1c.
abstract: In-house developed ELISA for HbA1c was evaluated in comparison with Bio-Rad D-10 Dual Program (HPLC). % HbA1c was estimated using said methods in 40 blood samples. Intra and inter assay coefficient of variation (CV) were in acceptable range i.e., <3%. HbA1c results obtained by two methods showed good correlation, (y = 0.8942x + 0.7229 R2 = 0.877). Sensitivity, specificity, positive, and negative predictive value for in-house ELISA classifying patients as controlled and uncontrolled diabetes against HPLC were recorded as 87%, 94.1%, 95.0%, and 93.8%, respectively, while accuracy of the test was 90.0%. In-house ELISA for HbA1c is not only accurate, cost effective and simple but also precise comparable to HPLC in deciding glycemic status of patients.
authors: PMRC Research Centre, Shaikh Zayed Medical Complex, Lahore, Pakistan. saima_razi@hotmail.com
authors: Saima Naz
authors: SN
authors: Mohsin
authors: Farkhanda
authors: F
authors: Ghafoor
authors: Muhammad
authors: M
authors: Aasim
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003920:Diabetes Mellitus / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006442:Glycated Hemoglobin / Q000378:metabolism*; D006801:Humans; D008297:Male
chemical_list: D006442:Glycated Hemoglobin; C517652:hemoglobin A1c protein, human