title: Purification of β-lactoglobulin with a high-capacity anion exchanger: high-throughput process development and scale-up considerations.
abstract: BACKGROUND
  β-Lactoglobulin is the most abundant protein in bovine whey. It is a valuable nutriceutical with multiple physiological functions. There are many ongoing efforts to improve approaches by which this whey protein can be conveniently and economically purified in significant quantities. High-capacity resins for protein fractionation are currently available in the biotech industry. One such resin is evaluated in the present investigation.
  
  
  RESULTS
  This work describes a high-capacity ion exchange chromatography method for one-column fractionation of β-lactoglobulin from whey. It was obtained with a >90% purity. The dynamic binding capacity was measured in packed columns. Comparable value predicted on the basis of Langmuir isotherm analysis from batch adsorption data in a high-throughput 96-well format is shown. Scale-up considerations are discussed with respect to feed concentration and binding capacity.
  
  
  CONCLUSIONS
  The feasibility of preparing purified β-lactoglobulin with a single high-capacity anion exchanger step was demonstrated. A capacity of >200 mg mL(-1) was obtained. A significant improvement in productivity can be realized by a simultaneous increase of binding capacity and feed concentration.
authors: Process Applications R&D, Process Chromatography Division, Bio-Rad Laboratories, Hercules, CA, 94547, USA.
authors: Paul K
authors: PK
authors: Ng
authors: Mark A
authors: MA
authors: Snyder
mesh_terms: D000818:Animals; D000837:Anion Exchange Resins; D002417:Cattle; D005591:Chemical Fractionation; D002852:Chromatography, Ion Exchange / Q000379:methods*; D007768:Lactalbumin / Q000302:isolation & purification; D007782:Lactoglobulins / Q000302:isolation & purification*; D008892:Milk / Q000737:chemistry*
chemical_list: D000837:Anion Exchange Resins; D007782:Lactoglobulins; D007768:Lactalbumin
keywords: high capacity; purification; whey; β-lactoglobulin