title: A shark liver gene-derived active peptide expressed in the silkworm, Bombyx mori: preliminary studies for oral administration of the recombinant protein.
abstract: Active peptide from shark liver (APSL) is a cytokine from Chiloscyllium plagiosum that can stimulate liver regeneration and protects the pancreas. To study the effect of orally administered recombinant APSL (rAPSL) on an animal model of type 2 diabetes mellitus, the APSL gene was cloned, and APSL was expressed in Bombyx mori N cells (BmN cells), silkworm larvae and silkworm pupae using the silkworm baculovirus expression vector system (BEVS). It was demonstrated that rAPSL was able to significantly reduce the blood glucose level in mice with type 2 diabetes induced by streptozotocin. The analysis of paraffin sections of mouse pancreatic tissues revealed that rAPSL could effectively protect mouse islets from streptozotocin-induced lesions. Compared with the powder prepared from normal silkworm pupae, the powder prepared from pupae expressing rAPSL exhibited greater protective effects, and these results suggest that rAPSL has potential uses as an oral drug for the treatment of diabetes mellitus in the future.
authors: Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Institute of Biochemistry, Zhejiang Sci-Tech University, Hangzhou 310018, China. liuyunlong5566@163.com
authors: Yunlong
authors: Y
authors: Liu
authors: Ying
authors: Y
authors: Chen
authors: Jianqing
authors: J
authors: Chen
authors: Wenping
authors: W
authors: Zhang
authors: Qing
authors: Q
authors: Sheng
authors: Jian
authors: J
authors: Chen
authors: Wei
authors: W
authors: Yu
authors: Zuoming
authors: Z
authors: Nie
authors: Yaozhou
authors: Y
authors: Zhang
authors: Wutong
authors: W
authors: Wu
authors: Lisha
authors: L
authors: Wang
authors: Inthrani Raja
authors: IR
authors: Indran
authors: Jun
authors: J
authors: Li
authors: Lian
authors: L
authors: Qian
authors: Zhengbing
authors: Z
authors: Lv
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D012831:Bombyx / Q000235:genetics / Q000378:metabolism; D016207:Cytokines / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000503:physiopathology; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy* / Q000503:physiopathology; D007515:Islets of Langerhans / Q000187:drug effects / Q000473:pathology; D008099:Liver; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011208:Powders; D011679:Pupa / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D012754:Sharks / Q000378:metabolism*; D013311:Streptozocin
chemical_list: D001786:Blood Glucose; D016207:Cytokines; D011208:Powders; D011994:Recombinant Proteins; D013311:Streptozocin
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