title: Polyionic and cysteine-containing fusion peptides as versatile protein tags.
abstract: In response to advances in proteomics research and the use of proteins in medical and biotechnological applications, recombinant protein production and the design of specific protein characteristics and functions has become a widely used technology. In this context, protein fusion tags have been developed as indispensable tools for protein expression, purification, and the design of functionalized surfaces or artificially bifunctional proteins. Here we summarize how positively or negatively charged polyionic fusion peptides with or without an additional cysteine can be used as protein tags for protein expression and purification, for matrix-assisted refolding of aggregated protein, and for coupling of proteins either to technologically relevant matrices or to other proteins. In this context we used cysteine-containing polyionic fusion peptides for the design of immunotoxins. In general, polyionic fusion tags can be considered as a multifunctional module in protein technology.
authors: Institut fur Biochemie und Biotechnologie, Martin-Luther-Universit at Halle-Wittenberg, Kurt-Mothes Strasse 3, D-06120 Halle/Saale, Germany. hauke.lilie@biochemtech.uni-halle.de
authors: Hauke
authors: H
authors: Lilie
authors: Susanne
authors: S
authors: Richter
authors: Sabine
authors: S
authors: Bergelt
authors: Stefan
authors: S
authors: Frost
authors: Franziska
authors: F
authors: Gehle
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular / Q000379:methods; D003545:Cysteine / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D000922:Immunotoxins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D007477:Ions / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D020673:Protein Renaturation; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D000922:Immunotoxins; D007477:Ions; D010455:Peptides; D011993:Recombinant Fusion Proteins; D003545:Cysteine