title: FBXO7 immunoreactivity in α-synuclein-containing inclusions in Parkinson disease and multiple system atrophy.
abstract: Mutations in the gene encoding the F-box only protein 7 (FBXO7) cause PARK15, an autosomal recessive form of juvenile parkinsonism. Although the brain pathology in PARK15 patients remains unexplored, in vivo imaging displays severe loss of nigrostriatal dopaminergic terminals. Understanding the pathogenesis of PARK15 might therefore illuminate the mechanisms of the selective dopaminergic neuronal degeneration, which could also be important for understanding idiopathic Parkinson disease (PD). The expression of FBXO7 in the human brain remains poorly characterized, and its expression in idiopathic PD and different neurodegenerative diseases has not been investigated. Here, we studied FBXO7 protein expression in brain samples of normal controls (n = 9) and from patients with PD (n = 13), multiple system atrophy (MSA) (n = 5), Alzheimer disease (AD) (n = 5), and progressive supranuclear palsy (PSP) (n = 5) using immunohistochemistry with 2 anti-FBXO7 antibodies. We detected widespread brain FBXO7 immunoreactivity, with the highest levels in neurons of the cerebral cortex, putamen, and cerebellum. There were no major differences between normal and PD brains overall, but FBXO7 immunoreactivity was detected in large proportions of α-synuclein-positive inclusions (Lewy bodies, Lewy neurites, glial cytoplasmic inclusions), where it colocalized with α-synuclein in PD and MSA cases. By contrast, weak FBXO7 immunoreactivity was occasionally detected in tau-positive inclusions in AD and PSP. These findings suggest a role for FBXO7 in the pathogenesis of the synucleinopathies.
authors: Departments of Clinical Genetics, Erasmus MC, Rotterdam, The Netherlands.
authors: Tianna
authors: T
authors: Zhao
authors: Lies-Anne
authors: LA
authors: Severijnen
authors: Marcel
authors: M
authors: van der Weiden
authors: Ping Pin
authors: PP
authors: Zheng
authors: Ben A
authors: BA
authors: Oostra
authors: Renate K
authors: RK
authors: Hukema
authors: Rob
authors: R
authors: Willemsen
authors: Johan M
authors: JM
authors: Kros
authors: Vincenzo
authors: V
authors: Bonifati
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001921:Brain / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D044783:F-Box Proteins / Q000276:immunology / Q000378:metabolism*; D005260:Female; D057809:HEK293 Cells; D006801:Humans; D002479:Inclusion Bodies / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D019578:Multiple System Atrophy / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D010300:Parkinson Disease / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D051844:alpha-Synuclein / Q000276:immunology / Q000378:metabolism*
chemical_list: D044783:F-Box Proteins; C489149:FBXO7 protein, human; D051844:alpha-Synuclein