title: [Imaging monitoring method of CaMKII activity by immunohistochemical analysis in schizophrenic model rats].
abstract: Schizophrenia is characterized by various behavioral abnormalities including cognitive dysfunction. Neonatal ventral hippocampus (NVH)-lesioned rats had been known as neurodevelopmental animal model similar to schizophrenia. Previous observations indicate that postpubertal NVH-lesioned rats exhibit impairments in prepulse inhibition (PPI), spontaneous locomotion, social interaction behavior and working memory. Here, we document the neurochemical basis of those defects in NVH-lesioned rats. Since Ca²⁺/calmodulin-dependent protein kinase II (CaMKII), which is NMDA receptor downstream kinase, is essential for memory and learning acquisition, we developed a protocol to monitor the spatial changes in CaMKII autophosphorylation using immunohistochemical imaging of whole brain slices with anti-autophosphorylated CaMKII antibody in order to address mechanisms underlying impaired cognitive function in NVH-lesioned rats. Immunohistochemical analyses using anti-autophosphorylated CaMKII antibody revealed that CaMKII autophosphorylation was significantly reduced in the medial prefrontal cortex (mPFC) of NVH-lesioned rats compared with control animals. This immunohistochemical technique is useful to investigate temporal and special changes in CaMKII activity in rodent brain and to evaluate drugs to improve the cognitive impairment.
authors: Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Tohoku University.
authors: Yasushi
authors: Y
authors: Yabuki
authors: Osamu
authors: O
authors: Nakagawasai
authors: Takeshi
authors: T
authors: Tadano
authors: Kohji
authors: K
authors: Fukunaga
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000378:metabolism*; D003071:Cognition / Q000502:physiology*; D003072:Cognition Disorders / Q000188:drug therapy / Q000209:etiology*; D004195:Disease Models, Animal; D006624:Hippocampus / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D008568:Memory; D057054:Molecular Imaging / Q000379:methods*; D008991:Monitoring, Physiologic / Q000379:methods*; D010766:Phosphorylation; D017397:Prefrontal Cortex / Q000378:metabolism; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism; D012559:Schizophrenia / Q000150:complications* / Q000378:metabolism* / Q000503:physiopathology; D012565:Schizophrenic Psychology
chemical_list: D016194:Receptors, N-Methyl-D-Aspartate; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2