title: Immunohistochemical analysis of staged guided bone regeneration and osseointegration of titanium implants using a polyethylene glycol membrane.
abstract: OBJECTIVE
  This study aimed to immunohistochemically evaluate staged guided bone regeneration and osseointegration of titanium implants using two bone graft substitutes in combination with a polyethylene glycol (PEG) membrane in a dog model.
  
  
  METHODS
  Saddle-type alveolar ridge defects were prepared in the lower jaws of 12 foxhounds and randomly filled with a natural bone mineral (NBM) or a biphasic calcium phosphate (SBC) and covered with an in situ gelling PEG membrane. After a healing period of 8 and 12 weeks (six animals each), modSLA titanium implants were inserted to heal in a submerged position. At 8 + 2 and 12 + 2 weeks, respectively, dissected blocks were processed for immunohistochemical analysis [osteocalcin (OC)].
  
  
  RESULTS
  After 8 + 2 weeks, mean OC values (%) tended to be higher in the NBM group (NBM, 32.7 ± 8.9%), but failed to reach statistical significance over the SBC group (SBC, 24.4 ± 6.6%). After 12 + 2 weeks, mean OC values decreased in both groups and was almost identical in both groups (NBM 1.6 ± 1.2%/SBC 2.1 ± 1.4%).
  
  
  CONCLUSIONS
  It was concluded that all augmentation procedures investigated were characterised by a comparable OC activity during the process of bone regeneration and osseointegration of modSLA titanium implants.
authors: Department of Oral Surgery, Westdeutsche Kieferklinik, Heinrich Heine University, 40225, Düsseldorf, Germany, ilja.mihatovic@med.uni-duesseldorf.de.
authors: Ilja
authors: I
authors: Mihatovic
authors: Vladimir
authors: V
authors: Golubovic
authors: Jürgen
authors: J
authors: Becker
authors: Frank
authors: F
authors: Schwarz
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D004285:Dogs; D007150:Immunohistochemistry; D008567:Membranes, Artificial; D016348:Osseointegration; D011092:Polyethylene Glycols; D019736:Prostheses and Implants; D014025:Titanium
chemical_list: D008567:Membranes, Artificial; D011092:Polyethylene Glycols; D014025:Titanium
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