title: Prevalence of fusB in Staphylococcus aureus clinical isolates.
abstract: Fusidic acid (FA) resistance in Staphylococcus aureus markedly varied among different regions. Few data for FA resistance are available in China. In this study, FA susceptibility testing was performed, and the prevalence of fusB and fusC in 116 clinical isolates of S. aureus was investigated by PCR. Mutations in fusA were also determined by sequencing of PCR products. Molecular typing of fusB-positive strains was based on multilocus sequence typing (MLST), spa typing and pulsed-field gel electrophoresis (PFGE). A DNA fragment flanking fusB was sequenced. Transformation experiments were carried out in fusB-positive S. aureus. Of 116 S. aureus including 19 meticillin-resistant S. aureus (MRSA) and 97 meticillin-susceptible S. aureus (MSSA), four (3.5 %) were resistant to FA with MICs of 6-12 µg ml(-1), including one MRSA from blood and three MSSA from wound exudates. All four FA-resistant isolates were found to be fusB gene positive. Three FA-resistant MSSA strains had the same MLST profile of ST630 and spa type of t377, whilst the MRSA strain belonged to ST630-t4549. Only one PFGE pattern was recognized for these four strains. No fusC and fusA mutations were detected in any of the isolates. FA resistance in fusB-positive clinical isolates could be transferred to S. aureus RN4220. The fusB gene was located in a transposon-like element, which had 99 % identity with that found in pUB101. In conclusion, the FA resistance rate is low in S. aureus, and the fusB gene is responsible for the resistance.
authors: Lishui Central Hospital, Zhejiang, PR China.|Key Laboratory of Clinical Pharmacology of Antibiotics, Ministry of Health, Shanghai, PR China.|Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, PR China.
authors: Jinwei
authors: J
authors: Huang
authors: Wenzhou Medical College, Zhejiang, PR China.
authors: Meiping
authors: M
authors: Ye
authors: Lishui Central Hospital, Zhejiang, PR China.
authors: Hui
authors: H
authors: Ding
authors: Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, PR China.
authors: Qinglan
authors: Q
authors: Guo
authors: Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, PR China.
authors: Baixing
authors: B
authors: Ding
authors: Key Laboratory of Clinical Pharmacology of Antibiotics, Ministry of Health, Shanghai, PR China.|Institute of Antibiotics, Huashan Hospital, Fudan University, Shanghai, PR China.
authors: Minggui
authors: M
authors: Wang
mesh_terms: D000328:Adult; D000368:Aged; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics*; D002681:China / Q000453:epidemiology; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D005672:Fusidic Acid / Q000494:pharmacology*; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D058885:Multilocus Sequence Typing; D009154:Mutation; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D015995:Prevalence; D017422:Sequence Analysis, DNA; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology*; D013205:Staphylococcal Protein A / Q000235:genetics; D013211:Staphylococcus aureus / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; C492443:FusB protein, Staphylococcus aureus; D013205:Staphylococcal Protein A; D005672:Fusidic Acid