title: Immobilization of proteins onto poly(2-hydroxyethyl methacrylate) functionalized Fe-Au/core-shell nanoparticles via adsorption strategy.
abstract: Biocompatible magnetic nanocomposites of Fe-AuNPs and poly(2-hydroxylethyl methacrylate) (PHEMA) were employed as a strategic protein immobilization platform. The hybrid magnetic nanocomposites were prepared by applying a 'grafting to' ATRP protocol. Fe-AuNPs having Fe core and Au shell were initially prepared by the inverse micelle method. Disulfide-containing PHEMA (DT-PHEMA) was grafted to the Fe-AuNPs surface by taking the advantages of the thiol chemistry. The grafting of DT-PHEMA to the Fe-AuNPs was confirmed by relevant spectroscopic analyses. The superparamagnetic property, a basic requirement for facile protein immobilization, of the magnetic nanocomposites was measured by the SQUID analysis. Lysozyme, gamma-globulins and bovine serum albumin (BSA) were immobilized onto magnetic nanocomposites via the adsorption strategy. The absorption intensity of lysozyme, gamma-globulins and BSA on the PHEMA grafted Fe-AuNPs were observed to be higher than that of bare Fe-AuNPs.
authors: Department of Imaging System Engineering, Pukyong National University, Busan, 608-737, Korea.
authors: M Rafiqul
authors: MR
authors: Islam
authors: Long Giang
authors: LG
authors: Bach
authors: Sung Yong
authors: SY
authors: Seo
authors: Kwon Taek
authors: KT
authors: Lim
mesh_terms: D000327:Adsorption; D006046:Gold / Q000737:chemistry*; D007501:Iron / Q000737:chemistry*; D008422:Materials Testing; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008968:Molecular Conformation; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry*; D016062:Porosity; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry* / Q000648:ultrastructure; D013499:Surface Properties
chemical_list: D011506:Proteins; D011102:Polyhydroxyethyl Methacrylate; D006046:Gold; D007501:Iron