title: Serum levels and activity of indoleamine2,3-dioxygenase and tryptophanyl-tRNA synthetase and their association with disease severity in patients with chronic kidney disease.
abstract: OBJECTIVE
  This study aims to test the serum levels and activity of indoleamine2,3-dioxygenase(IDO) and tryptophanyl-tRNA synthetase (TTS) in patients with chronic kidney disease (CKD) and to evaluate their association with disease severity.
  
  
  METHODS
  Serum concentrations of IDO and TTS in 61 patients with CKD and 16 healthy volunteers were tested by ELISA. Tryptophan and kynurenine concentrations were measured by high-performance liquid chromatography (HPLC).
  
  
  RESULTS
  Patients with CKD showed higher serum levels of IDO and TTS in comparison to healthy controls (p = 0.001). Patients with CKD showed lower serum levels of tryptophan and higher serum levels of kynurenine in comparison to healthy controls (p < 0.001). The kyn/Trp ratio significantly correlated with the disease severity in CKD patients (r = 0.721; p < 0.001).
  
  
  CONCLUSIONS
  IDO and TTS may play critical roles in the immune pathogenesis of CKD. The activity of IDO correlated with the disease severity of CKD.
authors: Department of Nephrology, The First Affiliated Hospital of Harbin Medical University, Harbin Medical University, Harbin, China.
authors: Yu-Shi
authors: YS
authors: Bao
authors: Ying
authors: Y
authors: Ji
authors: Shi-Lei
authors: SL
authors: Zhao
authors: Lin-Lin
authors: LL
authors: Ma
authors: Ru-Juan
authors: RJ
authors: Xie
authors: Shi-Ping
authors: SP
authors: Na
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D005919:Glomerular Filtration Rate; D006801:Humans; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000097:blood*; D007737:Kynurenine / Q000097:blood; D008297:Male; D008875:Middle Aged; D051436:Renal Insufficiency, Chronic / Q000097:blood* / Q000201:enzymology / Q000503:physiopathology; D014364:Tryptophan / Q000097:blood; D014369:Tryptophan-tRNA Ligase / Q000097:blood*
chemical_list: D015415:Biomarkers; C576710:IDO1 protein, human; C576711:IDO2 protein, human; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D007737:Kynurenine; D014364:Tryptophan; D014369:Tryptophan-tRNA Ligase