title: A mitochondria-targeting gold-peptide nanoassembly for enhanced cancer-cell killing.
abstract: Design and construction of multifunctional nanoparticles for effective delivery and therapeutic application remains a challenging task. It is desirable that nanoparticles can overcome multiple biological barriers and reach specific cellular locations to achieve maximum therapeutic effects. This aim often requires the fine tuning of nanoparticles' chemical and physical properties, as well as better understanding of their interaction with live cells. A peptide-modified gold-nanoparticle platform is designed, which consists of a 20-nm gold core stabilized with a layer of biotinylated CALNN-based peptides and a further layer of tetrameric streptavidins for functionalization with biotinylated molecules. The nanoassembly undergoes an efficient dynamin-dependent and caveolae-mediated endocytosis pathway, and displays highly specific localization to mitochondria, organelles of great therapeutic importance. When functionalized with a cytotoxic peptide (KLA: (KLAKLAK)2 ), the KLA-anchored nanoassembly exhibits dramatically enhanced anticancer activity, thousands of times stronger than that of the free KLA peptide, likely because of its improved cell entry efficiency, mitochondria-specific delivery, and the polyvalent effect of the nanoassembly. The study opens up the possibility of developing mitochondria-targeted nanomedicines.
authors: Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, 398 Ruoshui Road, Suzhou Industrial Park, Suzhou, Jiangsu, 215123, P. R. China; University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing, 100049, P. R. China.
authors: Xiaochuan
authors: X
authors: Ma
authors: Xiaobo
authors: X
authors: Wang
authors: Ming
authors: M
authors: Zhou
authors: Hao
authors: H
authors: Fei
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000737:chemistry* / Q000493:pharmacokinetics; D002470:Cell Survival / Q000187:drug effects; D016503:Drug Delivery Systems; D006046:Gold / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics; D006367:HeLa Cells; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D008928:Mitochondria / Q000378:metabolism*; D010455:Peptides / Q000008:administration & dosage* / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D036341:Intercellular Signaling Peptides and Proteins; C535058:KLA peptide; D010455:Peptides; D006046:Gold
keywords: anticancer; gold nanoparticles; mitochondria targeting; nanomedicine; polypeptides