title: The enhanced effect of lupeol on the destruction of gastric cancer cells by NK cells.
abstract: Lupeol, a triterpene, was reported to possess beneficial effects as a therapeutic and preventive agent for a range of disorders. Many studies have confirmed that lupeol possesses strong activities such as antioxidative, antiinflammatory, antiarthritic, antimutagenic, and antimalarial, both in vitro and in vivo, and at its effective therapeutic doses exhibit no toxicity to normal cells and tissues. Lupeol was observed to inhibit the proliferation of gastric tumour cells in a dose-dependent manner, as assessed by MTT assay, and induce the proliferation of NK cells, as assessed by flow cytometry and Western blotting. The killing effect of NK cells on gastric tumour cells was assessed by LDH. Our experiment demonstrated that lupeol at appropriate concentrations could promote the proliferation of NK cells, inhibit the proliferation of gastric cancer cell lines BGC823, N87 and HGC27, and increase the killing effect of NK cells on gastric cancer cells. We speculated that lupeol might increase the expression of PFP, IFN-γ, and CD107a via the activation of PI3K/Akt and Wnt/β-catenin signalling pathways. Lupeol could serve as a potential agent against gastric cancer; however, further in-depth in vivo studies are still required.
authors: Department of Physiology, Xuzhou Medical College, 84 West Huaihai Road, Xuzhou, Jiangsu 221002, China.
authors: Xiao-Ting
authors: XT
authors: Wu
authors: Jun-Quan
authors: JQ
authors: Liu
authors: Xiao-Ting
authors: XT
authors: Lu
authors: Fu-Xing
authors: FX
authors: Chen
authors: Zhong-Hai
authors: ZH
authors: Zhou
authors: Tao
authors: T
authors: Wang
authors: Sheng-Ping
authors: SP
authors: Zhu
authors: Su-Juan
authors: SJ
authors: Fei
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects / Q000276:immunology; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology; D007694:Killer Cells, Natural / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D052118:Lysosomal-Associated Membrane Protein 1 / Q000276:immunology; D053978:Pentacyclic Triterpenes / Q000494:pharmacology*; D052899:Pore Forming Cytotoxic Proteins / Q000276:immunology; D051057:Proto-Oncogene Proteins c-akt / Q000276:immunology; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000276:immunology*; D051176:beta Catenin / Q000276:immunology; D000094042:Lupanes
chemical_list: D000970:Antineoplastic Agents; D016207:Cytokines; D007371:Interferon-gamma; D052118:Lysosomal-Associated Membrane Protein 1; D053978:Pentacyclic Triterpenes; D052899:Pore Forming Cytotoxic Proteins; D051057:Proto-Oncogene Proteins c-akt; D051176:beta Catenin; C010480:lupeol; D000094042:Lupanes