title: Yeast diversity isolated from grape musts during spontaneous fermentation from a Brazilian winery.
abstract: Saccharomyces and non-Saccharomyces yeast species from a winery located in Brazil were identified by ribosomal gene-sequencing analysis. A total of 130 yeast strains were isolated from grape surfaces and musts during alcoholic fermentation from Isabel, Bordeaux, and Cabernet Sauvignon varieties. Samples were submitted to PCR-RFLP analysis and genomic sequencing. Thirteen species were identified: Candida quercitrusa, Candida stellata, Cryptococcus flavescens, Cryptococcus laurentii, Hanseniaspora uvarum, Issatchenkia occidentalis, Issatchenkia orientalis, Issatchenkia terricola, Pichia kluyveri, Pichia guilliermondii, Pichia sp., Saccharomyces cerevisiae, and Sporidiobolus pararoseus. A sequential substitution of species during the different stages of fermentation, with a dominance of non-Saccharomyces yeasts at the beginning, and a successive replacement of species by S. cerevisiae strains at the final steps were observed. This is the first report about the yeast distribution present throughout the alcoholic fermentation in a Brazilian winery, providing supportive information for future studies on their contribution to wine quality.
authors: Laboratory of Biochemistry and Applied Microbiology, São Paulo State University, São José do Rio Preto, São Paulo, 15054-000, Brazil. carol_bez@hotmail.com
authors: Carolina
authors: C
authors: Bezerra-Bussoli
authors: Milla Alves
authors: MA
authors: Baffi
authors: Eleni
authors: E
authors: Gomes
authors: Roberto
authors: R
authors: Da-Silva
mesh_terms: D044822:Biodiversity; D001938:Brazil; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D005285:Fermentation; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA; D027843:Vitis / Q000382:microbiology*; D014920:Wine / Q000382:microbiology*; D015003:Yeasts / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer
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