title: Increase of bone morphogenetic protein-7 expressing pulmonary resident cells in pneumonectomized rats.
abstract: OBJECTIVE
  Compensatory lung growth (CLG) is recognized in rodents subjected to major pulmonary resection; however, the source of cells constituting regenerated tissues during the CLG is still unknown. We investigated the differentiation of lung resident cells and the participation of bone marrow (BM)-derived cells in the remnant lung of pneumonectomized rats.
  
  
  METHODS
  After left pneumonectomy, the right remnant lung of Wistar rats was subjected to morphologic and molecular experiments at several time points. We studied the expression of bone morphogenic protein 7 (BMP-7), an accelerator of epithelial differentiation, based on the gene expression profile data of the remnant lung. Next, we evaluated the presence of GFP-positive cells in the remnant lung of Wistar rats that had received BM transplantation from green fluorescent protein (GFP) gene-transgenic Wistar rats prior to left pneumonectomy.
  
  
  RESULTS
  We observed progression of emphysematous change, modulation of gene expression profile, and proliferating cellular nuclear antigen-positive cells in the alveoli of the remnant lungs. BMP-7 protein positive cells were detected in the alveolar septa, which increased significantly over time with the progression of emphysematous change. No bone marrow-derived cells were detected in the right remnant lung of the GFP-BM transferred rats by fluorescence microscopy, immunohistochemistry, or polymerase chain reaction at any time.
  
  
  CONCLUSIONS
  Lung resident cells appear to contribute to CLG, possibly via a trans-differentiation pathway.
authors: Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, 812-8582, Japan, taro.oba@gmail.com.
authors: Taro
authors: T
authors: Ohba
authors: Hironobu
authors: H
authors: Wada
authors: Ichiro
authors: I
authors: Yoshino
authors: Shigetoshi
authors: S
authors: Yoshida
authors: Tetsuzo
authors: T
authors: Tagawa
authors: Fumihiro
authors: F
authors: Shoji
authors: Koji
authors: K
authors: Yamazaki
authors: Yoshihiko
authors: Y
authors: Maehara
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000502:physiology; D016026:Bone Marrow Transplantation; D055419:Bone Morphogenetic Protein 7 / Q000235:genetics* / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics*; D005260:Female; D015870:Gene Expression; D049452:Green Fluorescent Proteins; D008168:Lung / Q000166:cytology* / Q000502:physiology*; D011013:Pneumonectomy; D011650:Pulmonary Alveoli / Q000166:cytology / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration / Q000235:genetics* / Q000502:physiology*
chemical_list: D055419:Bone Morphogenetic Protein 7; D049452:Green Fluorescent Proteins
references: EMBO J. 1990 Jul;9(7):2085-93
references: 2357959
references: Am J Respir Crit Care Med. 2008 Feb 1;177(3):321-9
references: 17975199
references: Mech Dev. 1995 Mar;50(1):71-83
references: 7605753
references: FEBS Lett. 2004 Jan 2;556(1-3):249-52
references: 14706858
references: Am J Respir Cell Mol Biol. 2002 May;26(5):525-33
references: 11970903
references: Nat Rev Cancer. 2002 Jun;2(6):442-54
references: 12189386
references: J Cell Biol. 2006 Mar 27;172(7):973-81
references: 16567498
references: Cell. 2001 Jun 29;105(7):829-41
references: 11439179
references: Nat Med. 2000 Nov;6(11):1229-34
references: 11062533
references: Am J Physiol Lung Cell Mol Physiol. 2004 Apr;286(4):L658-67
references: 12922980
references: Nature. 2001 Apr 5;410(6829):701-5
references: 11287958
references: J Biol Chem. 2005 Mar 4;280(9):8094-100
references: 15591043
references: Acta Anat (Basel). 1995;154(1):8-20
references: 8714286
references: Am J Respir Cell Mol Biol. 1996 Nov;15(5):611-23
references: 8918368
references: Cell. 2001 May 4;105(3):369-77
references: 11348593
references: Am J Physiol Lung Cell Mol Physiol. 2006 Jan;290(1):L120-6
references: 16126788
references: Respir Res. 2001;2(6):340-7
references: 11737933
references: Genes Dev. 1995 Nov 15;9(22):2808-20
references: 7590255
references: Am J Physiol. 1988 Dec;255(6 Pt 1):C754-9
references: 3264460
references: J Thorac Cardiovasc Surg. 2012 Mar;143(3):711-719.e2
references: 22035964
references: Curr Opin Genet Dev. 1996 Aug;6(4):432-8
references: 8791534
references: Genes Cells. 2002 Dec;7(12):1191-204
references: 12485160
references: Nat Med. 2007 Aug;13(8):952-61
references: 17660828
references: Methods Enzymol. 2006;419:285-321
references: 17141060
references: Chest Surg Clin N Am. 1999 May;9(2):459-73, xii-xiii
references: 10365276
references: J Clin Invest. 2003 Dec;112(12):1776-84
references: 14679171
references: Science. 1998 Mar 6;279(5356):1528-30
references: 9488650
references: Pediatr Surg Int. 1998 Jul;13(5-6):341-5
references: 9639612
references: Respir Physiol. 2000 Jan;119(1):1-17
references: 10701703
references: Am J Respir Cell Mol Biol. 2004 Jan;30(1):76-83
references: 12829451
references: Development. 2001 Dec;128(24):5181-8
references: 11748153
references: FEBS Lett. 2003 Jul 3;546(1):133-9
references: 12829249
references: Comp Biochem Physiol A Mol Integr Physiol. 2001 May;129(1):245-60
references: 11369549
references: J Appl Physiol (1985). 2004 Nov;97(5):1992-8
references: 15475557
references: Am J Physiol Lung Cell Mol Physiol. 2002 Mar;282(3):L345-58
references: 11839527
references: J Clin Invest. 1980 Nov;66(5):962-70
references: 7430353
references: Scand J Thorac Cardiovasc Surg. 1982;16(3):293-8
references: 6820575
references: Genes Dev. 1995 Nov 15;9(22):2795-807
references: 7590254