title: Vertical distribution of ammonia-oxidizing archaea and bacteria in sediments of a eutrophic lake.
abstract: In order to characterize the vertical variation of abundance and community composition of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in sediments of a eutrophic lake, Lake Taihu, molecular techniques including real-time PCR, clone library, and sequencing were carried out in this study. Abundances of archaeal amoA gene (ranged from 2.34 × 10(6) to 4.43 × 10(7) copies [g dry sediment](-1)) were higher than those of bacterial amoA gene (ranged from 5.02 × 10(4) to 6.91 × 10(6) copies [g dry sediment](-1)) for all samples and both of them exhibited negative correlations with the increased depths. Diversities of archaeal and bacterial amoA gene increased with the elevated depths. There were no significant variations of AOB community structures derived from different sediment depths, whereas obvious differences were observed for the AOA community compositions. The information acquired in this study would be useful to elucidate the roles of AOA and AOB in the nitrogen cycling of freshwater ecosystems.
authors: State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering, Hohai University, Nanjing, 210098, China. dayong_zhao1982@126.com
authors: Dayong
authors: D
authors: Zhao
authors: Jin
authors: J
authors: Zeng
authors: Wenhua
authors: W
authors: Wan
authors: Huidi
authors: H
authors: Liang
authors: Rui
authors: R
authors: Huang
authors: Qinglong L
authors: QL
authors: Wu
mesh_terms: D000641:Ammonia / Q000378:metabolism*; D001105:Archaea / Q000145:classification* / Q000302:isolation & purification*; D001419:Bacteria / Q000145:classification* / Q000302:isolation & purification*; D058448:Biota; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D060106:Lakes / Q000382:microbiology*; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D019641:DNA, Archaeal; D004269:DNA, Bacterial; D000641:Ammonia; D010088:Oxidoreductases; C045582:ammonia monooxygenase
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