title: Ewing Sarcoma: influence of TP53 Arg72Pro and MDM2 T309G SNPs.
abstract: The Ewing Sarcoma is an important tumor of bone and soft tissue. The SNPs Arg72Pro of TP53 and T309G of MDM2 have been associated with many cancer types and have been differently distributed among populations worldwide. Based on a case-control design, this study aimed to assess the role of these SNPs in 24 Ewing Sarcoma patients, compared to 91 control individuals. DNA samples were extracted from blood and genotyped for both SNPs by PCR-RFLP and confirmed by DNA sequencing. The results showed an association between the G allele of the T309G and Ewing Sarcoma (P=0.02). Comparing to the TT carriers, the risk of G allele carriers was 3.35 (95% CI=1.22-9.21) with P=0.02. At the genotypic level, an association of the TT genotype with the control group (P=0.03) was found. Comparing to the TT genotype, the risk of TG and GG was 2.97 (95% CI=1.03-8.58) with P=0.04 and 5.00 (95% CI=1.23-20.34) with P=0.02, respectively. No associations regarding the Arg72Pro SNP were found. Considering that the T309G has been associated with several types of cancer, including sarcomas, our results indicate that this SNP may also be important to Ewing Sarcoma predisposition.
authors: Molecular and Cellular Oncology Research Group, Biotechnology Unit, Technology Development Center (CDTec), Federal University of Pelotas (UFPel), Pelotas, RS, 96010-900, Brazil.
authors: Helena S
authors: HS
authors: Thurow
authors: Fernando P
authors: FP
authors: Hartwig
authors: Clarice S
authors: CS
authors: Alho
authors: Deborah S B S
authors: DS
authors: Silva
authors: Rafael
authors: R
authors: Roesler
authors: Ana Lucia
authors: AL
authors: Abujamra
authors: Caroline Brunetto
authors: CB
authors: de Farias
authors: Algemir Lunardi
authors: AL
authors: Brunetto
authors: Bernardo L
authors: BL
authors: Horta
authors: Odir A
authors: OA
authors: Dellagostin
authors: Tiago
authors: T
authors: Collares
authors: Fabiana K
authors: FK
authors: Seixas
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics; D001938:Brazil; D016022:Case-Control Studies; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D016015:Logistic Models; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D051736:Proto-Oncogene Proteins c-mdm2 / Q000235:genetics*; D012512:Sarcoma, Ewing / Q000235:genetics*; D017422:Sequence Analysis, DNA; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; C497206:MDM2 protein, human; D051736:Proto-Oncogene Proteins c-mdm2
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