title: Exploring the utility of human DNA methylation arrays for profiling mouse genomic DNA.
abstract: Illumina Infinium Human Methylation (HM) BeadChips are widely used for measuring genome-scale DNA methylation, particularly in relation to epigenome-wide association studies (EWAS) studies. The methylation profile of human samples can be assessed accurately and reproducibly using the HM27 BeadChip (27,578 CpG sites) or its successor, the HM450 BeadChip (482,421 CpG sites). To date no mouse equivalent has been developed, greatly hindering the application of this methodology to the wide range of valuable murine models of disease and development currently in existence. We found 1308 and 13,715 probes from HM27 and HM450 BeadChip respectively, uniquely matched the bisulfite converted reference mouse genome (mm9). We demonstrate reproducible measurements of DNA methylation at these probes in a range of mouse tissue samples and in a murine cell line model of acute myeloid leukaemia. In the absence of a mouse counterpart, the Infinium Human Methylation BeadChip arrays have utility for methylation profiling in non-human species.
authors: Cancer and Disease Epigenetics Group, Murdoch Childrens Research Institute, Department of Paediatrics, The University of Melbourne, Royal Children's Hospital, Parkville, Victoria 3052, Australia. nwon@unimelb.edu.au
authors: Nicholas C
authors: NC
authors: Wong
authors: Jane
authors: J
authors: Ng
authors: Nathan E
authors: NE
authors: Hall
authors: Sebastian
authors: S
authors: Lunke
authors: Marika
authors: M
authors: Salmanidis
authors: Gabriela
authors: G
authors: Brumatti
authors: Paul G
authors: PG
authors: Ekert
authors: Jeffrey M
authors: JM
authors: Craig
authors: Richard
authors: R
authors: Saffery
mesh_terms: D000818:Animals; D018899:CpG Islands / Q000235:genetics*; D004247:DNA / Q000235:genetics*; D016172:DNA Fingerprinting; D019175:DNA Methylation / Q000235:genetics*; D015894:Genome, Human; D006801:Humans; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D004247:DNA