title: Identification of genes involved in Pseudomonas aeruginosa biofilm-specific resistance to antibiotics.
abstract: Pseudomonas aeruginosa is a key opportunistic pathogen characterized by its biofilm formation ability and high-level multiple antibiotic resistance. By screening a library of random transposon insertion mutants with an increased biofilm-specifc antibiotic susceptibility, we previously identified 3 genes or operons of P. aeruginosa UCBPP-PA14 (ndvB, PA1875-1877 and tssC1) that do not affect biofilm formation but are involved in biofilm-specific antibiotic resistance. In this study, we demonstrate that PA0756-0757 (encoding a putative two-component regulatory system), PA2070 and PA5033 (encoding hypothetical proteins of unknown function) display increased expression in biofilm cells and also have a role in biofilm-specific antibiotic resistance. Furthermore, deletion of each of PA0756, PA2070 and PA5033 resulted in a significant reduction of lethality in Caenorhabditis elegans, indicating a role for these genes in both biofilm-specific antibiotic resistance and persistence in vivo. Together, these data suggest that these genes are potential targets for antimicrobial agents.
authors: Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Canada.
authors: Li
authors: L
authors: Zhang
authors: Meredith
authors: M
authors: Fritsch
authors: Lisa
authors: L
authors: Hammond
authors: Ryan
authors: R
authors: Landreville
authors: Cristina
authors: C
authors: Slatculescu
authors: Antonio
authors: A
authors: Colavita
authors: Thien-Fah
authors: TF
authors: Mah
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D017173:Caenorhabditis elegans / Q000382:microbiology; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D015870:Gene Expression; D005798:Genes, Bacterial; D008826:Microbial Sensitivity Tests; D009154:Mutation; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development
chemical_list: D000900:Anti-Bacterial Agents
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