title: LSI-based amperometric sensor for real-time monitoring of embryoid bodies.
abstract: A large scale integration (LSI)-based amperometric sensor is used for electrochemical evaluation and real-time monitoring of the alkaline phosphatase (ALP) activity of mouse embryoid bodies (EBs). EBs were prepared by the hanging drop culture of embryonic stem (ES) cells. The ALP activity of EBs with various sizes was electrochemically detected at 400 measurement points on a Bio-LSI chip. The electrochemical measurements revealed that the relative ALP activity was low for large EBs and decreased with progress of the differentiation level of the ES cells. The ALP activity of the EBs was successfully monitored in real time for 3.5h, and their ALP activity in a glucose-free buffer decreased after 2h. To the best of our knowledge, this is the first report on the application of an LSI-based amperometric sensor for real-time cell monitoring over 3h. The chip is expected to be useful for the evaluation of cell activities.
authors: Graduate School of Environmental Studies, Tohoku University, Sendai 980-8579, Japan.
authors: Mustafa
authors: M
authors: Sen
authors: Kosuke
authors: K
authors: Ino
authors: Kumi Y
authors: KY
authors: Inoue
authors: Toshiharu
authors: T
authors: Arai
authors: Taku
authors: T
authors: Nishijo
authors: Atsushi
authors: A
authors: Suda
authors: Ryota
authors: R
authors: Kunikata
authors: Hitoshi
authors: H
authors: Shiku
authors: Tomokazu
authors: T
authors: Matsue
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism*; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation*; D002454:Cell Differentiation; D002470:Cell Survival; D055664:Electrochemical Techniques / Q000295:instrumentation*; D058732:Embryoid Bodies / Q000166:cytology / Q000201:enzymology*; D057075:Enzyme Assays / Q000295:instrumentation; D004867:Equipment Design; D051379:Mice
chemical_list: D000469:Alkaline Phosphatase