title: Implication of CA repeated tracts on post-transcriptional regulation in Trypanosoma cruzi.
abstract: In Trypanosoma cruzi gene expression regulation mainly relays on post-transcriptional events. Nevertheless, little is known about the signals which control mRNA abundance and functionality. We have previously found that CA repeated tracts (polyCA) are abundant in the vicinity of open reading frames and constitute specific targets for single stranded binding proteins from T. cruzi epimastigote. Given the reported examples of the involvement of polyCA motifs in gene expression regulation, we decided to further study their role in T. cruzi. Using an in silico genome-wide analysis, we identify the genes that contain polyCA within their predicted UTRs. We found that about 10% of T. cruzi genes carry polyCA therein. Strikingly, they are frequently concurrent with GT repeated tracts (polyGT), favoring the formation of a secondary structure exhibiting the complementary polydinucleotides in a double stranded helix. This feature is found in the species-specific family of genes coding for mucine associated proteins (MASPs) and other genes. For those polyCA-containing UTRs that lack polyGT, the polyCA is mainly predicted to adopt a single stranded structure. We further analyzed the functional role of such element using a reporter approach in T. cruzi. We found out that the insertion of polyCA at the 3' UTR of a reporter gene in the pTEX vector modulates its expression along the parasite's life cycle. While no significant change of the mRNA steady state of the reporter gene could be detected at the trypomastigote stage, significant increase in the epimastigote and reduction in the amastigote stage were observed. Altogether, these results suggest the involvement of polyCA as a signal in gene expression regulation in T. cruzi.
authors: Laboratorio de Interacciones Moleculares, Facultad de Ciencias, 11400 Montevideo, Uruguay.
authors: Lucía
authors: L
authors: Pastro
authors: Pablo
authors: P
authors: Smircich
authors: Leticia
authors: L
authors: Pérez-Díaz
authors: María Ana
authors: MA
authors: Duhagon
authors: Beatriz
authors: B
authors: Garat
mesh_terms: D016000:Cluster Analysis; D019295:Computational Biology; D018900:Dinucleotide Repeats / Q000235:genetics / Q000502:physiology*; D005786:Gene Expression Regulation / Q000235:genetics / Q000502:physiology*; D017930:Genes, Reporter; D009690:Nucleic Acid Conformation; D011061:Poly A / Q000235:genetics*; D011066:Poly C / Q000235:genetics*; D012323:RNA Processing, Post-Transcriptional / Q000502:physiology; D016053:RNA, Protozoan / Q000737:chemistry*; D014349:Trypanosoma cruzi / Q000235:genetics / Q000378:metabolism*; D020506:Untranslated Regions / Q000235:genetics / Q000502:physiology
chemical_list: D016053:RNA, Protozoan; D020506:Untranslated Regions; D011061:Poly A; D011066:Poly C
keywords: Polydinucleotides; RNA conformation; Reporter assay; UTR; mRNA stability; mRNA steady state