title: Fascin 1 is transiently expressed in mouse melanoblasts during development and promotes migration and proliferation.
abstract: Fascins, a family of actin-bundling proteins, are expressed in a spatially and temporally restricted manner during development and often in cancer. Fascin 1 has a clear role in cell migration in vitro, but its role in vivo in mammals is not well understood. Here, we investigate the role of fascin 1 in the melanocyte lineage and in melanoma cells. Fascin 1 knockout causes hypopigmentation in adult mice owing to migration and cell cycle progression defects in melanoblasts, the melanocyte precursor cell. Study of live embryo skin explants reveals that E14.5 fascin 1-null melanoblasts migrate slower, and generate fewer and thinner pseudopods. By contrast, fascin 1 expression drives faster migration and lamellipodia protrusion in melanocytes in vitro. In addition, fascin 1 depletion retards melanoblast proliferation in vivo and melanoma cell growth in vitro. These data indicate that fascin 1 not only promotes cell migration in mouse melanocytes but it also has a role in growth and cell cycle progression.
authors: Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, UK.
authors: Yafeng
authors: Y
authors: Ma
authors: Ang
authors: A
authors: Li
authors: William J
authors: WJ
authors: Faller
authors: Silvana
authors: S
authors: Libertini
authors: Florencia
authors: F
authors: Fiorito
authors: David A
authors: DA
authors: Gillespie
authors: Owen J
authors: OJ
authors: Sansom
authors: Shigeko
authors: S
authors: Yamashiro
authors: Laura M
authors: LM
authors: Machesky
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000502:physiology*; D002453:Cell Cycle; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D008544:Melanocytes / Q000166:cytology*; D008545:Melanoma / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D008840:Microfilament Proteins / Q000235:genetics / Q000502:physiology*; D010858:Pigmentation; D012867:Skin / Q000473:pathology; D013997:Time Factors
chemical_list: D002352:Carrier Proteins; D008840:Microfilament Proteins; C020324:fascin
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