title: Requirement of vesicle-associated membrane protein 721 and 722 for sustained growth during immune responses in Arabidopsis.
abstract: Extracellular immune responses to ascomycete and oomycete pathogens in Arabidopsis are dependent on vesicle-associated secretion mediated by the SNARE proteins PEN1 syntaxin, SNAP33 and endomembrane-resident VAMP721/722. Continuous movement of functional GFP-VAMP722 to and from the plasma membrane in non-stimulated cells reflects the second proposed function of VAMP721/722 in constitutive secretion during plant growth and development. Application of the bacterium-derived elicitor flg22 stabilizes VAMP721/722 that are otherwise constitutively degraded via the 26S proteasome pathway. Depletion of VAMP721/722 levels by reducing VAMP721/722 gene dosage enhances flg22-induced seedling growth inhibition in spite of elevated VAMP721/722 abundance. We therefore propose that plants prioritize the deployment of the corresponding secretory pathway for defense over plant growth. Interstingly, VAMP721/722 specifically interact in vitro and in vivo with the plasma membrane syntaxin SYP132 that is required for plant growth and resistance to bacteria. This suggests that the plant growth/immunity-involved VAMP721/722 form SNARE complexes with multiple plasma membrane syntaxins to discharge cue-dependent cargo molecules.
authors: Deparment of Plant Microbe Interactions, Max-Planck-Institut für Züchtungsforschung, D-50829 Köln, Germany.
authors: Hye Sup
authors: HS
authors: Yun
authors: Mark
authors: M
authors: Kwaaitaal
authors: Naohiro
authors: N
authors: Kato
authors: Changhyun
authors: C
authors: Yi
authors: Sohyeon
authors: S
authors: Park
authors: Masa H
authors: MH
authors: Sato
authors: Paul
authors: P
authors: Schulze-Lefert
authors: Chian
authors: C
authors: Kwon
mesh_terms: D017360:Arabidopsis / Q000276:immunology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D048708:Cell Growth Processes / Q000235:genetics / Q000276:immunology; D002462:Cell Membrane / Q000378:metabolism*; D002478:Cells, Cultured; D018628:Gene Dosage / Q000235:genetics; D007109:Immunity / Q000235:genetics; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D021381:Protein Transport / Q000235:genetics / Q000276:immunology; D050765:Qa-SNARE Proteins / Q000378:metabolism*; D050766:Qb-SNARE Proteins / Q000378:metabolism; D050767:Qc-SNARE Proteins / Q000378:metabolism; D050683:R-SNARE Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D036226:Seedlings / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins; C479425:PEN1 protein, Arabidopsis; D050765:Qa-SNARE Proteins; D050766:Qb-SNARE Proteins; D050767:Qc-SNARE Proteins; D050683:R-SNARE Proteins; C439366:SNAP33 protein, Arabidopsis; C568818:SYP132 protein, Arabidopsis; C570049:VAMP721 protein, Arabidopsis; C570050:VAMP722 protein, Arabidopsis; D046988:Proteasome Endopeptidase Complex; C061553:ATP dependent 26S protease
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