title: Surface modification of plasmonic nanostructured materials with thiolated oligonucleotides in 10 seconds using selective microwave heating.
abstract: This study demonstrates the proof-of-principle of rapid surface modification of plasmonic nanostructured materials with oligonucleotides using low power microwave heating. Due to their interesting optical and electronic properties, silver nanoparticle films (SNFs, 2 nm thick) deposited onto glass slides were used as the model plasmonic nanostructured materials. Rapid surface modification of SNFs with oligonucleotides was carried out using two strategies (1) Strategy 1: for ss-oligonucleotides, surface hybridization and (2) Strategy 2: for ds-oligonucleotides, solution hybridization), where the samples were exposed to 10, 15, 30 and 60 seconds microwave heating. To assess the efficacy of our new rapid surface modification technique, identical experiments carried out without the microwave heating (i.e., conventional method), which requires 24 hours for the completion of the identical steps. It was found that SNFs can be modified with ss- and ds-oligonucleotides in 10 seconds, which typically requires several hours of incubation time for the chemisorption of thiol groups on to the planar metal surface using conventional techniques.
authors: Morgan State University, Department of Chemistry, 1700 East Cold Spring Lane, Baltimore, MD 21251 USA.
authors: Biebele
authors: B
authors: Abel
authors: Kadir
authors: K
authors: Aslan
keywords: Surface modification; gold nanoparticles; metal-enhanced fluorescence; oligonucleotides; plasmonic nanostructures; plasmonics; silver island films
references: Biotechnol Prog. 2004 Nov-Dec;20(6):1817-24
references: 15575717
references: Anal Biochem. 2012 Feb 15;421(2):632-9
references: 22226791
references: Analyst. 2010 Aug;135(8):2028-35
references: 20593080
references: Nature. 1996 Aug 15;382(6592):607-9
references: 8757129
references: Analyst. 2012 Jan 7;137(1):202-8
references: 22049365
references: J Fluoresc. 2005 Mar;15(2):99-104
references: 15883762
references: Nano Biomed Eng. 2012 Jan 1;4(1):23-28
references: 22485194
references: J Mol Biol. 2005 May 6;348(3):589-96
references: 15826656
references: Macromol Rapid Commun. 2011 Apr 19;32(8):679-83
references: 21480427
references: Anal Chem. 2005 Dec 15;77(24):8057-67
references: 16351156
references: Anal Biochem. 1999 Oct 1;274(1):7-17
references: 10527491
references: Langmuir. 2012 Feb 28;28(8):3896-902
references: 22272583
references: Methods Mol Biol. 2012;800:33-53
references: 21964781
references: Nano Biomed Eng. 2011;3(3):184-191
references: 21949594
references: Nano Biomed Eng. 2010;2(1):1-9
references: 20622988
references: Expert Rev Mol Diagn. 2012 Jan;12(1):5-7
references: 22133112
references: Biosens Bioelectron. 2001 Dec;16(9-12):1109-18
references: 11679296
references: Anal Bioanal Chem. 2012 Jan;402(3):1093-100
references: 22124755
references: J Immunol Methods. 2008 Feb 29;331(1-2):103-13
references: 18230398
references: J Nanosci Nanotechnol. 2002 Jun-Aug;2(3-4):397-404
references: 12908269
references: J Am Chem Soc. 2012 May 2;134(17):7266-9
references: 22506486
references: Biochem Biophys Res Commun. 2003 Jun 20;306(1):213-8
references: 12788090
references: Nano Biomed Eng. 2010 Jan 1;2(3):155-164
references: 20953346
references: Angew Chem Int Ed Engl. 2007;46(26):4944-7
references: 17510988
references: Analyst. 2007 Nov;132(11):1122-9
references: 17955146
references: Biosens Bioelectron. 2010 Aug 15;25(12):2579-85
references: 20483582
references: Nat Biotechnol. 2001 Jan;19(1):51-5
references: 11135552
references: Analyst. 2007 Nov;132(11):1130-8
references: 17955147
references: Anal Chem. 2001 Jan 1;73(1):1-7
references: 11195491
references: Curr Opin Biotechnol. 2005 Feb;16(1):55-62
references: 15722016
references: Biochem Biophys Res Commun. 2006 Sep 22;348(2):612-7
references: 16890197
references: Anal Chem. 2009 Oct 15;81(20):8523-8
references: 19751052
references: Analyst. 2012 Mar 7;137(5):1259-64
references: 22282767
references: Anal Chem. 2011 Feb 1;83(3):674-7
references: 21218821
references: Nano Biomed Eng. 2010;2(3):165-170
references: 21949593
references: Anal Bioanal Chem. 2012 Jan;402(1):381-7
references: 21931953
references: Methods Mol Biol. 2012;808:183-94
references: 22057526
references: Anal Chem. 2006 Dec 15;78(24):8313-8
references: 17165821