title: A cryptic cytoplasmic male sterility unveils a possible gynodioecious past for Arabidopsis thaliana.
abstract: Gynodioecy, the coexistence of hermaphrodites and females (i.e. male-sterile plants) in natural plant populations, most often results from polymorphism at genetic loci involved in a particular interaction between the nuclear and cytoplasmic genetic compartments (cytonuclear epistasis): cytoplasmic male sterility (CMS). Although CMS clearly contributes to the coevolution of involved nuclear loci and cytoplasmic genomes in gynodioecious species, the occurrence of CMS genetic factors in the absence of sexual polymorphism (cryptic CMS) is not easily detected and rarely taken in consideration. We found cryptic CMS in the model plant Arabidopsis thaliana after crossing distantly related accessions, Sha and Mr-0. Male sterility resulted from an interaction between the Sha cytoplasm and two Mr-0 genomic regions located on chromosome 1 and chromosome 3. Additional accessions with either nuclear sterility maintainers or sterilizing cytoplasms were identified from crosses with either Sha or Mr-0. By comparing two very closely related cytoplasms with different male-sterility inducing abilities, we identified a novel mitochondrial ORF, named orf117Sha, that is most likely the sterilizing factor of the Sha cytoplasm. The presence of orf117Sha was investigated in worldwide natural accessions. It was found mainly associated with a single chlorotype in accessions belonging to a clade predominantly originating from Central Asia. More than one-third of accessions from this clade carried orf117Sha, indicating that the sterilizing-inducing cytoplasm had spread in this lineage. We also report the coexistence of the sterilizing cytoplasm with a non-sterilizing cytoplasm at a small, local scale in a natural population; in addition a correlation between cytotype and nuclear haplotype was detected in this population. Our results suggest that this CMS system induced sexual polymorphism in A. thaliana populations, at the time when the species was mainly outcrossing.
authors: INRA Institut National de la Recherche Agronomique, UMR1318, IJPB Institut Jean-Pierre Bourgin, Versailles, France.
authors: Nicolas
authors: N
authors: Gobron
authors: Cezary
authors: C
authors: Waszczak
authors: Matthieu
authors: M
authors: Simon
authors: Sophie
authors: S
authors: Hiard
authors: Stéphane
authors: S
authors: Boivin
authors: Delphine
authors: D
authors: Charif
authors: Aloïse
authors: A
authors: Ducamp
authors: Estelle
authors: E
authors: Wenes
authors: Françoise
authors: F
authors: Budar
mesh_terms: D017360:Arabidopsis / Q000145:classification / Q000235:genetics*; D005075:Biological Evolution; D002467:Cell Nucleus / Q000235:genetics; D032461:Chromosomes, Plant; D003433:Crosses, Genetic; D003593:Cytoplasm / Q000235:genetics; D056426:Genetic Loci; D008928:Mitochondria / Q000235:genetics*; D024101:Mitochondrial Proteins / Q000235:genetics*; D016366:Open Reading Frames; D010802:Phylogeny; D051479:Plant Infertility / Q000235:genetics*
chemical_list: D024101:Mitochondrial Proteins
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