title: Clinical significance of combined detection of human papilloma virus infection and human telomerase RNA component gene amplification in patients with squamous cell carcinoma of the esophagus in northern China.
abstract: BACKGROUND
  The aim of the study was to test for human papilloma virus (HPV) infection and human telomerase RNA component (hTERC) gene amplification in tissues derived from esophageal cancer, in esophagus displaying atypical hyperplasia and in normal tissue, and to analyze the relationship between them and discuss whether HPV infection and hTERC gene amplification play a role in the duration of survival of esophageal cancer patients.
  
  
  METHODS
  To test for HPV infection, surface plasma resonance was used after extracting and subjecting the DNA to PCR amplification. Measurement of hTERC gene amplification was performed by the fluorescence in situ hybridization technique.
  
  
  RESULTS
  The rates of HPV infection in the normal group, the atypical esophageal hyperplasia group and the cancer group were 0% (0/40), 10.00% (1/10) and 20.65% (19/92), respectively, with a statistically significant difference of P < 0.01. The hTERC gene amplification rate in normal tissue, grade I atypical hyperplastic tissue, grade II/III atypical hyperplastic tissue and esophageal cancer tissue were 0% (0/89), 15.38% (4/26), 47.06% (8/17) and 89.13% (82/92), respectively, with a statistically significant difference of P < 0.01. On follow-up of 92 patients, survival curves of the HPV-positive and HPV-negative groups were not significantly different (P > 0.05). Survival curves of the hTERC gene amplification-positive and hTERC gene amplification-negative groups were statistically significant (P < 0.05). A matching chi-square test showed that there was no correlation between HPV infection and hTERC gene amplification (P > 0.05).
  
  
  CONCLUSIONS
  HPV infection may be one of many factors contributing to the development of esophageal cancer, but it does not influence prognosis. Amplification of the hTERC gene appears to influence certain features associated with postoperative survival in esophageal carcinoma patients.
authors: Department of Oncology, First Affiliated Hospital, Cancer Institute, Henan University of Science and Technology, Luoyang, Henan Province, 471003, China.
authors: Yu-Feng
authors: YF
authors: Wang
authors: Xin-Shuai
authors: XS
authors: Wang
authors: She-Gan
authors: SG
authors: Gao
authors: Qiang
authors: Q
authors: Chen
authors: Yan-Tong
authors: YT
authors: Yang
authors: Zhong-Yue
authors: ZY
authors: Xiao
authors: Xiu-Qing
authors: XQ
authors: Peng
authors: Xiu-Feng
authors: XF
authors: Hu
authors: Qia-Yin
authors: QY
authors: Wang
authors: Xiao-Shan
authors: XS
authors: Feng
mesh_terms: D000328:Adult; D002294:Carcinoma, Squamous Cell / Q000150:complications / Q000235:genetics* / Q000821:virology*; D002681:China; D004938:Esophageal Neoplasms / Q000150:complications / Q000175:diagnosis / Q000235:genetics* / Q000821:virology*; D005260:Female; D005784:Gene Amplification; D006801:Humans; D008297:Male; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000150:complications / Q000175:diagnosis* / Q000235:genetics; D012313:RNA / Q000235:genetics*; D016019:Survival Analysis; D019098:Telomerase / Q000235:genetics*
chemical_list: C422952:telomerase RNA; D012313:RNA; D019098:Telomerase
references: BMC Genomics. 2010 Oct 18;11:576
references: 20955586
references: J Pathol. 2006 Dec;210(4):412-9
references: 17054308
references: Mol Cell. 2009 Nov 25;36(4):609-19
references: 19941821
references: J Infect Dis. 1994 Feb;169(2):235-40
references: 8106758
references: J Huazhong Univ Sci Technolog Med Sci. 2009 Jun;29(3):368-71
references: 19513624
references: Acta Cytol. 2012;56(1):80-4
references: 22236750
references: Gulf J Oncolog. 2011 Jul;(10):18-26
references: 21724525
references: Oncol Rep. 2003 Sep-Oct;10(5):1431-6
references: 12883719
references: Cancer Epidemiol Biomarkers Prev. 2012 May;21(5):780-5
references: 22337534
references: World J Gastroenterol. 2006 Mar 7;12(9):1352-5
references: 16552800
references: Int J Hyg Environ Health. 2011 Mar;214(2):156-61
references: 21130683
references: J Neurovirol. 2003 Feb;9(1):29-35
references: 12587066
references: BMC Cancer. 2010 Jan 16;10:19
references: 20078887
references: Gynecol Obstet Invest. 1982;14(2):90-6
references: 6288519
references: Harefuah. 2011 Nov;150(11):837-41, 876
references: 22428203
references: Int J Cancer. 2006 Sep 15;119(6):1354-9
references: 16615110
references: Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2011 Feb;25(1):39-41
references: 21789852