title: Combined meningococcal serogroup A and W135 outer-membrane vesicles activate cell-mediated immunity and long-term memory responses against non-covalent capsular polysaccharide A.
abstract: Outer-membrane vesicles (OMVs) have inherent adjuvant properties, and many vaccines use OMV as vaccine components. Utilizing the adjuvant properties of OMV could lead to the formulation of vaccines that are less expensive and potentially more immunogenic than covalently conjugated polysaccharide vaccines. We evaluated the adjuvant effect in Balb/c mice of combinations of OMV from Neisseria meningitidis serogroup A and W135 as compared to that of the non-covalently conjugated capsular polysaccharide A. Both antigens were adsorbed onto aluminum hydroxide. The mice were given a booster dose of plain polysaccharide A to stimulate an immunologic memory response. Subclasses determination and cytokine assays demonstrated the capacity of OMV to induce a IgG2a/IgG2b isotype profile and IFN-γ production, suggesting the induction of a Th1 pattern immune response. Lymphoproliferative responses to OMVs were high, with affinity maturation of antibodies observed. Bactericidal titers after the booster dose were also observed. Memory B cells and long-term memory T cells were also detected. The results of this study indicate that combined meningococcal serogroup A and W135 OMV can activate cell-mediated immunity and induce a long-term memory response.
authors: Immunology Department, Vice presidency of Research and Development, Havana, Cuba, bromeu@finlay.edu.cu.
authors: Belkis
authors: B
authors: Romeu
authors: Miriam
authors: M
authors: Lastre
authors: Luis
authors: L
authors: García
authors: Bárbara
authors: B
authors: Cedré
authors: Aleida
authors: A
authors: Mandariote
authors: Mildrey
authors: M
authors: Fariñas
authors: Reynaldo
authors: R
authors: Oliva
authors: Einar
authors: E
authors: Rosenqvist
authors: Oliver
authors: O
authors: Pérez
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D005260:Female; D007111:Immunity, Cellular / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007156:Immunologic Memory / Q000276:immunology; D007371:Interferon-gamma / Q000097:blood / Q000276:immunology; D008585:Meningitis, Meningococcal / Q000276:immunology* / Q000517:prevention & control; D022401:Meningococcal Vaccines / Q000276:immunology* / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D038381:Neisseria meningitidis, Serogroup A / Q000276:immunology*; D038582:Neisseria meningitidis, Serogroup W-135 / Q000276:immunology*; D011135:Polysaccharides, Bacterial / Q000276:immunology; D050683:R-SNARE Proteins / Q000276:immunology*; D013047:Specific Pathogen-Free Organisms
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D022401:Meningococcal Vaccines; D011135:Polysaccharides, Bacterial; D050683:R-SNARE Proteins; D007371:Interferon-gamma
references: Rev Cubana Med Trop. 2004 Jan-Apr;56(1):26-30
references: 15849905
references: J Infect Dis. 1998 Jun;177(6):1614-21
references: 9607841
references: Methods. 2009 Dec;49(4):301-8
references: 19410000
references: Mucosal Immunol. 2013 Mar;6(2):288-96
references: 22806100
references: Infect Immun. 2001 Jul;69(7):4502-8
references: 11401992
references: Pediatr Infect Dis J. 2004 Aug;23(8):748-55
references: 15295225
references: J Leukoc Biol. 2004 Feb;75(2):163-89
references: 14525967
references: Vaccine. 2008 Aug 18;26(35):4434-45
references: 18617296
references: J Immunol. 2005 Nov 1;175(9):5675-80
references: 16237057
references: Vaccine. 2005 Jan 26;23(10):1312-21
references: 15652674
references: Annu Rev Immunol. 2005;23:487-513
references: 15771579
references: Expert Rev Vaccines. 2011 Mar;10(3):307-22
references: 21434799
references: J Infect Dis. 2003 Apr 1;187(7):1142-6
references: 12660929
references: J Infect Dis. 1998 Apr;177(4):1112-5
references: 9534995
references: Eur J Pharm Sci. 2011 Sep 18;44(1-2):32-40
references: 21699977
references: NIPH Ann. 1991 Dec;14(2):195-207; discussion 208-10
references: 1812432
references: Scand J Immunol. 2012 Aug;76(2):99-107
references: 22537024
references: Cell Microbiol. 2011 Jul;13(7):925-33
references: 21501363
references: Lancet. 1985 Jul 20;2(8447):114-8
references: 2862316
references: J Infect Dis. 2010 Aug 15;202(3):470-9
references: 20575660
references: Infect Immun. 1999 Jan;67(1):113-9
references: 9864204
references: Semin Immunol. 2004 Oct;16(5):323-33
references: 15528077
references: Vaccine. 2010 Feb 25;28(9):2097-103
references: 20056181
references: Vaccine. 2005 May 31;23(29):3762-74
references: 15893613
references: Immunol Cell Biol. 2004 Dec;82(6):603-10
references: 15550118
references: Nat Med. 2005 Apr;11(4 Suppl):S54-62
references: 15812491
references: Vaccine. 2004 Mar 29;22(11-12):1423-32
references: 15063565
references: Vaccine. 2009 Dec 11;28(2):576-82
references: 19878751
references: Pediatr Infect Dis J. 2007 Aug;26(8):716-22
references: 17848884
references: Vaccine. 2001 Apr 30;19(23-24):3043-50
references: 11311998
references: PLoS One. 2012;7(2):e31160
references: 22347446
references: Pediatr Infect Dis J. 1992 Oct;11(10):855-9
references: 1408486
references: Scand J Infect Dis. 2011 Oct;43(10):809-13
references: 21671827
references: Nat Rev Immunol. 2009 Mar;9(3):213-20
references: 19214194
references: Vaccine. 2004 Jun 2;22(17-18):2171-80
references: 15149774
references: Carbohydr Res. 2003 Nov 14;338(23):2539-47
references: 14670715
references: Vaccine. 2009 Jun 24;27 Suppl 2:B3-12
references: 19481313
references: Nat Med. 2011 Nov 20;17(12):1602-9
references: 22101769
references: J Exp Med. 2006 Feb 20;203(2):305-10
references: 16476769
references: Annu Rev Immunol. 2010;28:107-30
references: 19968562