title: Detection of orally administered inositol stereoisomers in mouse blood plasma and their effects on translocation of glucose transporter 4 in skeletal muscle cells.
abstract: Simple pharmacological studies on inositol stereoisomers are presented in this study. Male ICR mice were orally administered 1 g/kg BW of three inositol stereoisomers, myo-inositol (MI), d-chiro-inositol (DCI), and scyllo-inositol (SI), and blood plasma samples and skeletal muscle fractions were prepared after an hour. The plasma samples were subjected to gas chromatography-coupled time-of-flight mass spectrometry (GC-TOF-MS) analysis. None of the three stereoisomers was seen in untreated samples, but substantial amounts ranging from 2.5 to 6.5 mM were detected only after administration, indicating that orally administered inositol stereoisomers were readily absorbed and their levels elevated in the bloodstream. In addition, plasma of SI-administered animals contained substantial MI, suggesting a possible metabolic conversion of SI to MI. In the skeletal muscle fractions, glucose transporter type 4 (GLUT4) content in the plasma membrane increased, indicating that inositol stereoisomers stimulated GLUT4 translocation.
authors: Department of Agrobioscience and ‡Organization of Advanced Science and Technology, Kobe University , 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.
authors: Yoko
authors: Y
authors: Yamashita
authors: Masaru
authors: M
authors: Yamaoka
authors: Tomohisa
authors: T
authors: Hasunuma
authors: Hitoshi
authors: H
authors: Ashida
authors: Ken-ichi
authors: K
authors: Yoshida
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D051275:Glucose Transporter Type 4 / Q000378:metabolism*; D007294:Inositol / Q000008:administration & dosage* / Q000097:blood* / Q000493:pharmacokinetics; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000648:ultrastructure; D021381:Protein Transport / Q000187:drug effects; D013237:Stereoisomerism
chemical_list: D051275:Glucose Transporter Type 4; C009217:scyllitol; D007294:Inositol