title: Effect of hydrogen peroxide on immersion challenge of rainbow trout fry with Flavobacterium psychrophilum.
abstract: An experimental model for immersion challenge of rainbow trout fry (Oncorhynchus mykiss) with Flavobacterium psychrophilum, the causative agent of rainbow trout fry syndrome and bacterial cold water disease was established in the present study. Although injection-based infection models are reliable and produce high levels of mortality attempts to establish a reproducible immersion model have been less successful. Various concentrations of hydrogen peroxide (H₂O₂) were evaluated before being used as a pre-treatment stressor prior to immersion exposure to F. psychrophilum. H₂O₂ accelerated the onset of mortality and increased mortality approximately two-fold; from 9.1% to 19.2% and from 14.7% to 30.3% in two separate experiments. Clinical signs observed in the infected fish corresponded to symptoms characteristically seen during natural outbreaks. These findings indicate that pre-treatment with H₂O₂ can increase the level of mortality in rainbow trout fry after exposure to F. psychrophilum.
authors: National Veterinary Institute, Technical University of Denmark, Bülowsvej 27, Frederiksberg C, Denmark. mmah@vet.dtu.dk
authors: Maya Maria Mihályi
authors: MM
authors: Henriksen
authors: Lone
authors: L
authors: Madsen
authors: Inger
authors: I
authors: Dalsgaard
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D005393:Fish Diseases / Q000382:microbiology*; D045826:Flavobacteriaceae Infections / Q000382:microbiology*; D005417:Flavobacterium / Q000187:drug effects* / Q000502:physiology*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D007101:Immersion; D017686:Oncorhynchus mykiss / Q000382:microbiology*
chemical_list: D000891:Anti-Infective Agents, Local; D006861:Hydrogen Peroxide
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