title: Effect of white adipose tissue flap and insulin-like growth factor-1 on nerve regeneration in rats.
abstract: Adipose tissue-derived stem cells and insulin-like growth factor-1 (IGF-1) have shown potential to enhance peripheral nerve regeneration. The purpose of this study was to investigate the effect of an in vivo biologic scaffold, consisting of white adipose tissue flap (WATF) and/or IGF-1 on nerve regeneration in a crush injury model. Forty rats all underwent a sciatic nerve crush injury and then received: a pedicled WATF, a controlled local release of IGF-1, both treatments, or no treatment at the injury site. Outcomes were the normalized maximum isometric tetanic force (ITF) of the tibialis anterior muscle and histomorphometric measurements. At 4 weeks, groups with WATF had a statistically significant improvement in maximum ITF recovery, as compared to those without (P < 0.05), and there was an increase in myelin thickness and total axon count in the WATF-only group versus control (P < 0.01). Functional and histomorphometric data suggest that IGF-1 suppressed the effect of the WATF. Use of a pedicled WATF improved the functional and histomorphometrical results after axonotmesis in a rat model. IGF-1 does not appear to enhance nerve regeneration in this model. Utilizing the WATF may have a beneficial therapeutic role in peripheral nerve injuries.
authors: Department of Orthopaedic Surgery, Columbia University, New York, NY, USA.
authors: Ayhan
authors: A
authors: Kilic
authors: Bukola
authors: B
authors: Ojo
authors: Rebecca A
authors: RA
authors: Rajfer
authors: Geoffrey
authors: G
authors: Konopka
authors: Daniel
authors: D
authors: Hagg
authors: Eugene
authors: E
authors: Jang
authors: Yelena
authors: Y
authors: Akelina
authors: Jeremy J
authors: JJ
authors: Mao
authors: Melvin P
authors: MP
authors: Rosenwasser
authors: Peter
authors: P
authors: Tang
mesh_terms: D052436:Adipose Tissue, White; D000818:Animals; D001696:Biomechanical Phenomena; D003131:Combined Modality Therapy; D003692:Delayed-Action Preparations; D048091:Guided Tissue Regeneration / Q000379:methods*; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D008863:Microspheres; D009416:Nerve Regeneration / Q000187:drug effects / Q000502:physiology; D059348:Peripheral Nerve Injuries / Q000188:drug therapy / Q000601:surgery / Q000628:therapy*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000187:drug effects / Q000293:injuries* / Q000473:pathology / Q000502:physiology; D013524:Surgical Flaps; D054457:Tissue Scaffolds; D016896:Treatment Outcome
chemical_list: D003692:Delayed-Action Preparations; D007334:Insulin-Like Growth Factor I