title: The protective effect of recombinant FomA-expressing Lactobacillus acidophilus against periodontal infection.
abstract: A number of studies have shown that the outer membrane protein FomA found in Fusobacterium nucleatum demonstrates great potential as an immune target for combating periodontitis. Lactobacillus acidophilus is a useful antigen delivery vehicle for mucosal immunisation, and previous studies by our group have shown that L. acidophilus acts as a protective factor in periodontal health. In this study, making use of the immunogenicity of FomA and the probiotic properties of L. acidophilus, we constructed a recombinant form of L. acidophilus expressing the FomA protein and detected the FomA-specific IgG in the serum and sIgA in the saliva of mice through oral administration with the recombinant strains. When serum containing FomA-specific antibodies was incubated with the F. nucleatum in vitro, the number of Porphyromonas gingivalis cells that coaggregated with the F. nucleatum cells was significantly reduced. Furthermore, a mouse gum abscess model was successfully generated, and the range of gingival abscesses in the immune mice was relatively limited compared with the control group. The level of IL-1β in the serum and local gum tissues of the immune mice was consistently lower than in the control group. Our findings indicated that oral administration of the recombinant L. acidophilus reduced the risk of periodontal infection with P. gingivalis and F. nucleatum.
authors: Department of Preventive and Pediatric Dentistry, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, 200011, China.
authors: Li
authors: L
authors: Ma
authors: Qinfeng
authors: Q
authors: Ding
authors: Xiping
authors: X
authors: Feng
authors: Fei
authors: F
authors: Li
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D016866:Bacteroidaceae Infections / Q000276:immunology / Q000517:prevention & control / Q000628:therapy*; D004195:Disease Models, Animal; D005260:Female; D005674:Fusobacterium Infections / Q000276:immunology / Q000517:prevention & control / Q000628:therapy*; D016967:Fusobacterium nucleatum / Q000276:immunology / Q000472:pathogenicity; D007070:Immunoglobulin A / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D053583:Interleukin-1beta / Q000097:blood / Q000378:metabolism; D007779:Lactobacillus acidophilus / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D010508:Periodontal Abscess / Q000382:microbiology / Q000517:prevention & control / Q000628:therapy*; D016966:Porphyromonas gingivalis / Q000276:immunology / Q000472:pathogenicity; D019936:Probiotics / Q000627:therapeutic use
chemical_list: D001425:Bacterial Outer Membrane Proteins; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D053583:Interleukin-1beta; C083760:fomA protein, Fusobacterium
references: Infect Immun. 2010 Mar;78(3):1185-92
references: 20008529
references: J Clin Periodontol. 2006 Mar;33(3):226-32
references: 16489950
references: Infect Immun. 2001 Mar;69(3):1547-53
references: 11179325
references: FEMS Microbiol Lett. 2003 Dec 5;229(1):119-26
references: 14659551
references: Caries Res. 2004 May-Jun;38(3):204-11
references: 15153690
references: J Basic Microbiol. 2007 Jun;47(3):281-6
references: 17518422
references: Benef Microbes. 2010 Mar;1(1):11-29
references: 21831747
references: Vaccine. 2010 Apr 26;28(19):3496-505
references: 20189489
references: Trends Microbiol. 2003 Feb;11(2):94-100
references: 12598132
references: Eur J Biochem. 1995 Oct 1;233(1):310-6
references: 7588760
references: Arch Oral Biol. 2007 Jul;52(7):697-704
references: 17275778
references: Int J Mol Sci. 2012;13(2):1582-97
references: 22408409
references: J Nutr. 2001 Mar;131(3):807-12
references: 11238763
references: Lett Appl Microbiol. 1999 Jun;28(6):419-22
references: 10389256
references: FEMS Microbiol Lett. 2000 Jan 1;182(1):57-62
references: 10612731
references: PLoS One. 2011;6(10):e26163
references: 22022548
references: Eur J Dent. 2010 Jul;4(3):348-55
references: 20613927
references: J Bacteriol. 1993 Feb;175(3):840-50
references: 8380804
references: Inflammation. 2012 Aug;35(4):1330-7
references: 22382516
references: Shanghai Kou Qiang Yi Xue. 2011 Aug;20(4):364-7
references: 21909593
references: Infect Immun. 1998 Oct;66(10):4729-32
references: 9746571
references: Oral Microbiol Immunol. 1988 Dec;3(4):145-52
references: 3254470
references: Arch Oral Biol. 1992 Sep;37(9):717-24
references: 1329700
references: J Appl Microbiol. 2006 Feb;100(2):256-63
references: 16430501
references: Clin Microbiol Rev. 1996 Jan;9(1):55-71
references: 8665477
references: Immunol Lett. 2005 Mar 15;97(2):181-8
references: 15752556
references: Infect Immun. 1989 Feb;57(2):331-7
references: 2912893
references: FEMS Microbiol Lett. 2005 Jan 1;242(1):27-35
references: 15675069
references: Curr Drug Metab. 2009 Jan;10(1):90-4
references: 19149517
references: Acta Odontol Scand. 2012 Jan;70(1):1-6
references: 21449691
references: Infect Immun. 1997 Dec;65(12):5231-7
references: 9393820
references: Eur J Biochem. 1999 Mar;260(3):818-24
references: 10103012
references: Microb Cell Fact. 2005 Jan 04;4(1):2
references: 15631634