title: Myf5 expression during fetal myogenesis defines the developmental progenitors of adult satellite cells.
abstract: Myf5 is a member of the muscle-specific determination genes and plays a critical role in skeletal muscle development. Whereas the expression of Myf5 during embryonic and fetal myogenesis has been extensively studied, its expression in progenitors that will ultimately give rise to adult satellite cells, the stem cells responsible for muscle repair, is still largely unexplored. To investigate this aspect, we have generated a mouse strain carrying a CreER coding sequence in the Myf5 locus. In this strain, Tamoxifen-inducible Cre activity parallels endogenous Myf5 expression. Combining Myf5(CreER) and Cre reporter alleles, we were able to evaluate the contribution of cells expressing Myf5 at distinct developmental stages to the pool of satellite cells in adult hindlimb muscles. Although it was possible to trace back the origin of some rare satellite cells to a subpopulation of Myf5(+ve) progenitors in the limb buds at the late embryonic stage (∼E12), a significant number of satellite cells arise from cells which expressed Myf5 for the first time at the fetal stage (∼E15). These studies provide direct evidence that adult satellite cells derive from progenitors that first express the myogenic determination gene Myf5 during fetal stages of myogenesis.
authors: Paul F. Glenn Laboratories for the Biology of Aging and Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA 94305, USA.
authors: Stefano
authors: S
authors: Biressi
authors: Christopher R R
authors: CR
authors: Bjornson
authors: Poppy M M
authors: PM
authors: Carlig
authors: Koichi
authors: K
authors: Nishijo
authors: Charles
authors: C
authors: Keller
authors: Thomas A
authors: TA
authors: Rando
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D019070:Cell Lineage / Q000502:physiology*; D017931:DNA Primers / Q000235:genetics; D005333:Fetus / Q000502:physiology*; D005434:Flow Cytometry; D005697:Galactosides; D007211:Indoles; D019426:Integrases; D051379:Mice; D008856:Microscopy, Fluorescence; D024510:Muscle Development / Q000502:physiology*; D018482:Muscle, Skeletal / Q000166:cytology*; D051787:Myogenic Regulatory Factor 5 / Q000378:metabolism*; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology / Q000378:metabolism*; D013234:Stem Cells / Q000378:metabolism*; D013629:Tamoxifen
chemical_list: D017931:DNA Primers; D005697:Galactosides; D007211:Indoles; C497418:Myf5 protein, mouse; D051787:Myogenic Regulatory Factor 5; D013629:Tamoxifen; C045073:Cre recombinase; D019426:Integrases; C044888:5-bromo-4-chloro-3-indolyl beta-galactoside
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