title: Migrating oligodendrocyte progenitor cells swell prior to soma dislocation.
abstract: The migration of oligodendrocyte progenitor cells (OPCs) to the white matter is an indispensable requirement for an intact brain function. The mechanism of cell migration in general is not yet completely understood. Nevertheless, evidence is accumulating that besides the coordinated rearrangement of the cytoskeleton, a finetuned interplay of ion and water fluxes across the cell membrane is essential for cell migration. One part of a general hypothesis is that a local volume increase towards the direction of movement triggers a mechano-activated calcium influx that regulates various procedures at the rear end of a migrating cell. Here, we investigated cell volume changes of migrating OPCs using scanning ion conductance microscopy. We found that during accelerated migration OPCs undergo an increase in the frontal cell body volume. These findings are supplemented with time lapse calcium imaging data that hint an increase in calcium content the frontal part of the cell soma.
authors: Central Unit for Ionbeams and Radionuclides (RUBION), Ruhr-University Bochum, Bochum, Germany. patrick.happel@rub.de
authors: Patrick
authors: P
authors: Happel
authors: Kerstin
authors: K
authors: Möller
authors: Nina K
authors: NK
authors: Schwering
authors: Irmgard D
authors: ID
authors: Dietzel
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D002454:Cell Differentiation; D002465:Cell Movement / Q000502:physiology*; D002467:Cell Nucleus / Q000378:metabolism; D048429:Cell Size; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000378:metabolism*; D040542:Mechanotransduction, Cellular; D020527:Microscopy, Scanning Probe / Q000295:instrumentation / Q000379:methods; D057054:Molecular Imaging / Q000379:methods; D009836:Oligodendroglia / Q000166:cytology / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013234:Stem Cells / Q000166:cytology / Q000502:physiology*
chemical_list: D002118:Calcium
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