title: Enhanced expression of MYF5 and MYOD1 in fibroblast cells via the forced expression of bos taurus MYF5.
abstract: The formation of vertebrate skeletal muscles widely thought to be under the control of hierarchy of regulatory genes. MYF5 is one of the myogenic determination gene expressed in the developing mouse dermomyotome which control skeletal muscle differentiation. In the current work, we had obtained the cDNA sequence including the full coding region of the bos taurus myogenic factor MYF5 by reverse transcription polymerase chain reaction. Furthermore, we examined whether fibroblast cell derived from mouse and bos taurus can be transduced using plasmid vectors carrying bos taurus MYF5. Bos taurus MYF5 activates MYF5 and MYOD1 expression after 1 day culture. The concerted upregulation of the myogenic regulatory factors enhanced myosin (skeletal fast) expression. These observation show that MYF5 is essential for myogenic differentiation and provides candidates for regulation bos taurus skeletal muscle development.
authors: Laboratory of Animal Genetics and Breeding, Department of Animal Science, Tianjin Agricultural University, Tianjin, 300384, P. R. China.
authors: Yong Wei
authors: YW
authors: Nie
authors: Xiang Bin
authors: XB
authors: Ding
authors: Xiu Guo
authors: XG
authors: Ge
authors: Han Lu
authors: HL
authors: Fan
authors: Zhong Wei
authors: ZW
authors: Liu
authors: Hong
authors: H
authors: Guo
mesh_terms: D000818:Animals; D002417:Cattle; D002454:Cell Differentiation; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D015870:Gene Expression; D051379:Mice; D024510:Muscle Development / Q000235:genetics; D018482:Muscle, Skeletal / Q000166:cytology / Q000378:metabolism*; D017570:MyoD Protein / Q000235:genetics* / Q000378:metabolism; D051787:Myogenic Regulatory Factor 5 / Q000235:genetics* / Q000378:metabolism; D016366:Open Reading Frames; D010957:Plasmids; D014161:Transduction, Genetic
chemical_list: D017570:MyoD Protein; C057363:MyoD1 myogenic differentiation protein; D051787:Myogenic Regulatory Factor 5
keywords: bos taurus MYF5; fibroblast cell transduced; muscle differentiation