title: Suppression of murine collagen-induced arthritis by vaccination of synovial vascular endothelial cells.
abstract: OBJECTIVE
  Endothelial cells (ECs) lining the lumina of blood vessels are involved in leukocyte extravasation underlying inflammatory states, such as rheumatoid arthritis (RA). The rheumatoid pannus, the site of inflammation and joint destruction in the rheumatoid synovium, relies on the development of neovascular vessels to sustain its growth. We studied a method to selectively target and destroy new synovial blood vessels by vaccination with synovial EC.
  
  
  METHODS
  Collagen-induced arthritis (CIA) mice were vaccinated with tumor necrosis factor (TNF)-alpha-stimulated EC (TNF-EC) antigen with an incomplete adjuvant. TNF-EC was used as a model of EC in synovial tissue on RA.
  
  
  RESULTS
  Arthritis was significantly decreased in TNF-EC vaccinated mice compared with non-vaccinated mice based on the arthritis score. Moreover, the TNF-EC vaccine suppressed bone erosion, hyperplasia of the synovium and expression of neovascular vessel as shown by hematoxylin-eosin staining, X-ray analysis and immunohistochemical.
  
  
  CONCLUSIONS
  Vaccine therapy against vascular EC in synovial tissue may provide a novel approach to the treatment of RA.
authors: Laboratory of Drug and Gene Delivery, Faculty of Pharma-sciences, Teikyo University, 2-11-1 Kaga, Itabashi-ku, Tokyo 173-8605, Japan. sawaguti@pharm.teikyo-u.ac.jp
authors: Yoshikazu
authors: Y
authors: Sawaguchi
authors: Keiichi
authors: K
authors: Hirata
authors: Ryo
authors: R
authors: Suzuki
authors: Naoki
authors: N
authors: Utoguchi
authors: Kazuo
authors: K
authors: Maruyama
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D000941:Antigens / Q000276:immunology* / Q000494:pharmacology; D001169:Arthritis, Experimental / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D024043:Collagen Type II / Q000276:immunology; D004730:Endothelium, Vascular / Q000166:cytology* / Q000276:immunology / Q000473:pathology; D008297:Male; D051379:Mice; D008811:Mice, Inbred DBA; D009389:Neovascularization, Pathologic / Q000276:immunology / Q000517:prevention & control; D013583:Synovial Membrane / Q000166:cytology* / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology; D014611:Vaccination / Q000379:methods*
chemical_list: D000906:Antibodies; D000941:Antigens; D024043:Collagen Type II; D014409:Tumor Necrosis Factor-alpha