title: A magneto-DNA nanoparticle system for rapid detection and phenotyping of bacteria.
abstract: So far, although various diagnostic approaches for pathogen detection have been proposed, most are too expensive, lengthy or limited in specificity for clinical use. Nanoparticle systems with unique material properties, however, circumvent these problems and offer improved accuracy over current methods. Here, we present novel magneto-DNA probes capable of rapid and specific profiling of pathogens directly in clinical samples. A nanoparticle hybridization assay, involving ubiquitous and specific probes that target bacterial 16S rRNAs, was designed to detect amplified target DNAs using a miniaturized NMR device. Ultimately, the magneto-DNA platform will allow both universal and specific detection of various clinically relevant bacterial species, with sensitivity down to single bacteria. Furthermore, the assay is robust and rapid, simultaneously diagnosing a panel of 13 bacterial species in clinical specimens within 2 h. The generic platform described could be used to rapidly identify and phenotype pathogens for a variety of applications.
authors: Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge Street, CPZN 5206, Boston, Massachusetts 02114, USA.
authors: Hyun Jung
authors: HJ
authors: Chung
authors: Cesar M
authors: CM
authors: Castro
authors: Hyungsoon
authors: H
authors: Im
authors: Hakho
authors: H
authors: Lee
authors: Ralph
authors: R
authors: Weissleder
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques / Q000379:methods*; D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D060328:Magnetic Phenomena; D008969:Molecular Sequence Data; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010641:Phenotype; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012015:Reference Standards; D013045:Species Specificity
chemical_list: D012336:RNA, Ribosomal, 16S; D004247:DNA
references: Nature. 2009 Nov 26;462(7272):461-4
references: 19940916
references: Nat Biotechnol. 2005 Jul;23(7):885-9
references: 15951805
references: Pediatrics. 2012 Apr;129(4):e1104-9
references: 22451708
references: Trends Biotechnol. 2011 May;29(5):240-50
references: 21377748
references: ACS Nano. 2011 Nov 22;5(11):8834-41
references: 21967150
references: Emerg Infect Dis. 2007 Apr;13(4):594-600
references: 17553275
references: Clin Infect Dis. 2009 May 1;48(9):1268-75
references: 19335166
references: Expert Rev Med Devices. 2009 Nov;6(6):641-51
references: 19911875
references: Nat Biotechnol. 2012 May;30(5):434-9
references: 22522955
references: Lancet. 2011 Jan 15;377(9761):228-41
references: 21146207
references: Nat Med. 2008 Aug;14(8):869-74
references: 18607350
references: Sci Transl Med. 2012 Jul 4;4(141):141ra92
references: 22764208
references: J Clin Microbiol. 2002 Sep;40(9):3449-54
references: 12202592
references: Clin Microbiol Infect. 2008 Oct;14(10):908-34
references: 18828852
references: Science. 2006 Dec 1;314(5804):1464-7
references: 17138901
references: Nat Nanotechnol. 2010 Sep;5(9):660-5
references: 20676091
references: Nat Biotechnol. 2002 Aug;20(8):816-20
references: 12134166
references: Annu Rev Food Sci Technol. 2011;2:259-79
references: 22129383
references: Clin Chem. 2009 May;55(5):856-66
references: 19264858
references: Science. 2005 Apr 22;308(5721):554-7
references: 15845853
references: Proc Natl Acad Sci U S A. 2009 Jul 28;106(30):12459-64
references: 19620715
references: Angew Chem Int Ed Engl. 2012 Jul 27;51(31):7752-5
references: 22744868
references: Nat Rev Microbiol. 2012 Sep;10(9):599-606
references: 22864262
references: Nat Rev Microbiol. 2010 Jan;8(1):74-82
references: 20010952
references: Nature. 2000 Aug 17;406(6797):799-803
references: 10963611
references: Nature. 1990 May 3;345(6270):63-5
references: 1691827
references: J Appl Microbiol. 2010 Mar;108(3):779-788
references: 19702857
references: Microbiol Res. 2011 Feb 20;166(2):99-110
references: 20223646
references: J Microbiol Methods. 2009 Sep;78(3):325-30
references: 19616588
references: Bioconjug Chem. 1999 Mar-Apr;10(2):186-91
references: 10077466
references: Lab Chip. 2011 Jul 7;11(13):2282-7
references: 21547317
references: Crit Care. 2009;13(4):217
references: 19664174