title: Determination of the major phenolic compounds in pomegranate juices by HPLC−DAD−ESI-MS.
abstract: Traditionally, pomegranate (Punica granatum L.) has been consumed as fresh fruit or as pomegranate juice. In this study, the main phenolic compounds of 12 pomegranate varieties and 5 pomegranate clones were determined by HPLC−DAD−ESI-MS. Two chromatographic methods with a fused-core C18 column and a classical HPLC system were developed. Thirteen anthocyanins and fourteen other phenolic compounds were determined in the pomegranate juices. As far as we are concerned, a new flavonol-glycoside, phellatin or its isomer amurensin, has been tentatively identified for the first time in pomegranate juices. Total phenolic content ranged from 580.8 to 2551.3 mg/L of pomegranate juice. Anthocyanins varied between 20 to 82% of total phenolic content. Flavonoids were 1.6-23.6% of total phenolic compounds, while phenolic acids and ellagitannins were in the range 16.4-65.8%. The five clones reported a phenolic content comparable with that of the other pomegranate samples.
authors: Department of Analytical Chemistry, University of Granada, c/Fuentenueva s/n, 18071, Granada, Spain.
authors: Ana María
authors: AM
authors: Gómez-Caravaca
authors: Vito
authors: V
authors: Verardo
authors: Moreno
authors: M
authors: Toselli
authors: Antonio
authors: A
authors: Segura-Carretero
authors: Alberto
authors: A
authors: Fernández-Gutiérrez
authors: Maria Fiorenza
authors: MF
authors: Caboni
mesh_terms: D000872:Anthocyanins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D000975:Antioxidants / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D019976:Cloning, Organism; D055664:Electrochemical Techniques; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D005638:Fruit / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D055951:Functional Food / Q000032:analysis*; D047348:Hydrolyzable Tannins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D029561:Lythraceae / Q000737:chemistry* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D019083:Mediterranean Region; D015394:Molecular Structure; D010636:Phenols / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000872:Anthocyanins; D000975:Antioxidants; D005419:Flavonoids; D047348:Hydrolyzable Tannins; D010636:Phenols