title: Novel method for transdiaphragmatic pressure measurements in mice.
abstract: The diaphragm muscle (DIAm) is responsible for breathing and determines the ability to generate both ventilatory and non-ventilatory behaviors. Size limitations of the mouse make transdiaphragmatic pressure (Pdi) measurement using a dual balloon system untenable. Adult C57BL/6J mice (n=8) and C57BL/6×129 (n=9), underwent Pdi measurements using solid-state pressure catheters spanning the thoracic and abdominal surfaces of the DIAm. Measurements were conducted during eupnea, hypoxia (10% O2)-hypercapnia (5% CO2), chemical airway stimulation (i.e., sneezing), spontaneously occurring deep breaths, sustained tracheal occlusion, and bilateral phrenic nerve stimulation. There was a difference in the Pdi generated across the range of ventilatory and non-ventilatory behaviors (p=0.001). No difference in Pdi across behaviors was evident between mouse strains (p=0.161). This study establishes a novel method to determine Pdi across a range of DIAm behaviors in mice that may be useful in evaluating conditions associated with reduced ability to perform expulsive, non-ventilatory behaviors.
authors: Department of Physiology and Biomedical Engineering, Mayo Clinic, College of Medicine, 200 First Street SW, Rochester, MN 55905, USA.
authors: Sarah M
authors: SM
authors: Greising
authors: Dylan C
authors: DC
authors: Sieck
authors: Gary C
authors: GC
authors: Sieck
authors: Carlos B
authors: CB
authors: Mantilla
mesh_terms: D000818:Animals; D003964:Diaphragm / Q000502:physiology*; D008297:Male; D051379:Mice; D057507:Mice, 129 Strain; D008810:Mice, Inbred C57BL; D011312:Pressure; D012129:Respiratory Function Tests / Q000379:methods*; D015656:Respiratory Mechanics / Q000502:physiology*
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