title: Comparative analysis of serum proteins in relation to rheumatoid arthritis and chronic periodontitis.
abstract: BACKGROUND
  Rheumatoid arthritis (RA) and chronic periodontitis (CP) are chronic inflammatory conditions and share many pathologic features. The common molecular pathogenesis of the two inflammatory diseases is unclear. The aim of the present study is to evaluate serum protein profiles specific for patients with RA and CP by a comprehensive proteomic analysis.
  
  
  METHODS
  The study participants were: 10 patients with RA, 10 patients with CP, 10 patients with RA and CP, and 10 healthy controls. All groups were balanced for age, sex, and smoking status. Serum protein spot volume was examined with two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis. Proteins with significant differences in abundance among the four groups were determined with computer image analysis and identified with mass spectrometry and protein databases.
  
  
  RESULTS
  A total of 1,694 protein spots were obtained in sera of the four groups. Seven spots were significantly different in abundance among the four groups. Of these, three spots (complement component 3, complement factor H, and ceruloplasmin) were significantly different in the RA+CP group compared with the other three groups (P <0.05). The similar profiles of complement component 3, complement factor H, and ceruloplasmin were observed by enzyme-linked immunosorbent assay.
  
  
  CONCLUSIONS
  These results suggest that patients with RA and CP may exhibit three serum proteins with different abundance compared with healthy controls and patients with RA only or CP only.
authors: Division of Periodontology, Department of Oral Biological Science, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
authors: Tomoko
authors: T
authors: Yokoyama
authors: Tetsuo
authors: T
authors: Kobayashi
authors: Satoshi
authors: S
authors: Ito
authors: Akira
authors: A
authors: Yamagata
authors: Kohei
authors: K
authors: Ishida
authors: Moe
authors: M
authors: Okada
authors: Ken
authors: K
authors: Oofusa
authors: Akira
authors: A
authors: Murasawa
authors: Hiromasa
authors: H
authors: Yoshie
mesh_terms: D001172:Arthritis, Rheumatoid / Q000097:blood*; D001798:Blood Proteins / Q000032:analysis*; D016022:Case-Control Studies; D002570:Ceruloplasmin / Q000032:analysis; D055113:Chronic Periodontitis / Q000097:blood* / Q000145:classification; D003176:Complement C3 / Q000032:analysis; D017242:Complement Factor H / Q000032:analysis; D051056:Complement Inactivating Agents / Q000097:blood; D003774:Dental Plaque Index; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D007155:Immunologic Factors / Q000097:blood; D007525:Isoelectric Focusing / Q000379:methods; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D017622:Periodontal Attachment Loss / Q000145:classification; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000145:classification
chemical_list: D001798:Blood Proteins; D003176:Complement C3; D051056:Complement Inactivating Agents; D007155:Immunologic Factors; D017242:Complement Factor H; D002570:Ceruloplasmin