title: Plasma proteins adsorption mechanism on polyethylene-grafted poly(ethylene glycol) surface by quartz crystal microbalance with dissipation.
abstract: Protein adsorption has a vital role in biomaterial surface science because it is directly related to the hemocompatibility of blood-contacting materials. In this study, monomethoxy poly(ethylene glycol) (mPEG) with two different molecular weights was grafted on polyethylene as a model to elucidate the adsorption mechanisms of plasma protein through quartz crystal microbalance with dissipation (QCM-D). Combined with data from platelet adhesion, whole blood clotting time, and hemolysis rate, the blood compatibility of PE-g-mPEG film was found to have significantly improved. Two adsorption schemes were developed for real-time monitoring of protein adsorption. Results showed that the preadsorbed bovine serum albumin (BSA) on the surfaces of PE-g-mPEG films could effectively inhibit subsequent adsorption of fibrinogen (Fib). Nonspecific protein adsorption of BSA was determined by surface coverage, not by the chain length of PEG. Dense PEG brush could release more trapped water molecules to resist BSA adsorption. Moreover, the preadsorbed Fib could be gradually displaced by high-concentration BSA. However, the adsorption and displacement of Fib was determined by surface hydrophilicity.
authors: State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China.
authors: Jing
authors: J
authors: Jin
authors: Wei
authors: W
authors: Jiang
authors: Jinghua
authors: J
authors: Yin
authors: Xiangling
authors: X
authors: Ji
authors: Paola
authors: P
authors: Stagnaro
mesh_terms: D000327:Adsorption; D000818:Animals; D001792:Blood Platelets / Q000166:cytology / Q000187:drug effects; D001798:Blood Proteins / Q000737:chemistry*; D002417:Cattle; D002448:Cell Adhesion; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects; D005340:Fibrinogen / Q000037:antagonists & inhibitors / Q000737:chemistry; D006461:Hemolysis; D057927:Hydrophobic and Hydrophilic Interactions; D008970:Molecular Weight; D020959:Polyethylene; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000494:pharmacology; D058525:Quartz Crystal Microbalance Techniques / Q000379:methods*; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013499:Surface Properties; D014914:Whole Blood Coagulation Time
chemical_list: D001798:Blood Proteins; D012710:Serum Albumin, Bovine; D011092:Polyethylene Glycols; D005340:Fibrinogen; D020959:Polyethylene; C028210:monomethoxypolyethylene glycol