title: Biodegradation of tetrahydrofuran by Pseudomonas oleovorans DT4 immobilized in calcium alginate beads impregnated with activated carbon fiber: mass transfer effect and continuous treatment.
abstract: A novel entrapment matrix, calcium alginate (CA) coupled with activated carbon fiber (ACF), was prepared to immobilize Pseudomonas oleovorans DT4 for degrading tetrahydrofuran (THF). The addition of 1.5% ACF increased the adsorption capacity of the immobilized bead, thus resulting in an enhanced average removal rate of 30.3mg/(Lh). The synergism between adsorption and biodegradation was observed in the hybrid CA-ACF beads instead of in the system comprising CA beads and freely suspended ACF. The effective diffusion coefficient of the CA-ACF bead was not significantly affected by bead size, but the bead's value of 1.14×10(-6)cm(2)/s (for the bead diameter of 0.4 cm) was larger than that of the CA bead by almost one order of magnitude based on the intraparticle diffusion-reaction kinetics analysis. Continuous treatment of the THF-containing wastewater was succeeded by CA-ACF immobilized cells in a packed-bed reactor for 54 d with a >90% removal efficiency.
authors: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China.
authors: Dong-Zhi
authors: DZ
authors: Chen
authors: Jun-Yi
authors: JY
authors: Fang
authors: Qian
authors: Q
authors: Shao
authors: Jie-Xu
authors: JX
authors: Ye
authors: Du-Juan
authors: DJ
authors: Ouyang
authors: Jian-Meng
authors: JM
authors: Chen
mesh_terms: D000327:Adsorption; D000464:Alginates / Q000494:pharmacology*; D001673:Biodegradation, Environmental / Q000187:drug effects; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D002244:Carbon / Q000494:pharmacology*; D000077482:Carbon Fiber; D018914:Cells, Immobilized / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002606:Charcoal / Q000494:pharmacology*; D004275:DNA, Ribosomal / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D004058:Diffusion; D005663:Furans / Q000378:metabolism*; D020723:Glucuronic Acid / Q000494:pharmacology; D006603:Hexuronic Acids / Q000494:pharmacology; D008863:Microspheres; D044204:Pseudomonas oleovorans / Q000166:cytology* / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D012996:Solutions
chemical_list: D000464:Alginates; D000077482:Carbon Fiber; D004275:DNA, Ribosomal; D005663:Furans; D006603:Hexuronic Acids; D012996:Solutions; D002606:Charcoal; C018674:tetrahydrofuran; D002244:Carbon; D020723:Glucuronic Acid