title: Septin9 is involved in T-cell development and CD8+ T-cell homeostasis.
abstract: SEPTIN9 (SEPT9) is a filament-forming protein involved in numerous cellular processes. We have used a conditional knock out allele of Sept9 to specifically delete Sept9 in T-cells. As shown by fluorescence-activated cell sorting, loss of Sept9 at an early thymocyte stage in the thymus results in increased numbers of double-negative cells indicating that SEPT9 is involved in the transition from the double-negative stage during T-cell development. Accordingly, the relative numbers of mature T-cells in the periphery are decreased in mice with a T-cell-specific deletion of Sept9. Proliferation of Sept9-deleted CD8(+) T-cells from the spleen is decreased upon stimulation in culture. The altered T-cell homeostasis caused by the loss of Sept9 results in an increase of CD8(+) central memory T-cells.
authors: Department of Molecular Biology and Genetics, Aarhus University, Bldg. 1130, C.F. Mollers Allé 3, 8000, Aarhus, Denmark.
authors: Louise Berkhoudt
authors: LB
authors: Lassen
authors: Annette
authors: A
authors: Füchtbauer
authors: Alexander
authors: A
authors: Schmitz
authors: Annette Balle
authors: AB
authors: Sørensen
authors: Finn Skou
authors: FS
authors: Pedersen
authors: Ernst-Martin
authors: EM
authors: Füchtbauer
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology* / Q000378:metabolism*; D002452:Cell Count; D002454:Cell Differentiation / Q000235:genetics; D049109:Cell Proliferation; D015536:Down-Regulation / Q000235:genetics; D006706:Homeostasis / Q000276:immunology*; D007156:Immunologic Memory / Q000235:genetics; D019426:Integrases / Q000378:metabolism; D008212:Lymphocyte Depletion; D008221:Lymphoid Tissue / Q000166:cytology / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D060888:Real-Time Polymerase Chain Reaction; D058112:Septins / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation / Q000235:genetics
chemical_list: C045073:Cre recombinase; D019426:Integrases; D058112:Septins; C574402:septin 9, mouse