title: Comparative bioenergetic study of neuronal and muscle mitochondria during aging.
abstract: Mitochondrial respiratory chain defects have been associated with various diseases and with normal aging, particularly in tissues with high energy demands, including brain and skeletal muscle. Tissue-specific manifestation of mitochondrial DNA (mtDNA) mutations and mitochondrial dysfunction are hallmarks of mitochondrial diseases although the underlying mechanisms are largely unclear. Previously, we and others have established approaches for transferring mtDNA from muscle and synaptosomes of mice at various ages to cell cultures. In this study, we carried out a comprehensive bioenergetic analysis of cells bearing mitochondria derived from young, middle-aged, and old mouse skeletal muscles and synaptosomes. Significant age-associated alterations in oxidative phosphorylation and regulation during aging were observed in cybrids carrying mitochondria from both skeletal muscle and synaptosomes. Our results also revealed that loss of oxidative phosphorylation capacity may occur at various ages in muscle and brain. These findings indicate the existence of a tissue-specific regulatory mechanism for oxidative phosphorylation.
authors: School of Life Science, Wenzhou Medical College, Wenzhou, Zhejiang 325035, China.
authors: Hongzhi
authors: H
authors: Li
authors: Lokendra
authors: L
authors: Kumar Sharma
authors: Youfen
authors: Y
authors: Li
authors: Peiqing
authors: P
authors: Hu
authors: Abimbola
authors: A
authors: Idowu
authors: Danhui
authors: D
authors: Liu
authors: Jianxin
authors: J
authors: Lu
authors: Yidong
authors: Y
authors: Bai
mesh_terms: D000375:Aging / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D001921:Brain / Q000378:metabolism / Q000473:pathology; D004272:DNA, Mitochondrial; D004734:Energy Metabolism; D006801:Humans; D051379:Mice; D008928:Mitochondria / Q000378:metabolism* / Q000473:pathology; D028361:Mitochondrial Diseases / Q000378:metabolism* / Q000473:pathology; D009154:Mutation; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D009928:Organ Specificity; D010085:Oxidative Phosphorylation
chemical_list: D004272:DNA, Mitochondrial
keywords: Aging; Cybrid; Free radicals; Mitochondria; Skeletal muscle; Synaptosome
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