title: Use of laser fluorescence in dental caries diagnosis: a fluorescence x biomolecular vibrational spectroscopic comparative study.
abstract: The aim of this work was to verify the existence of correlation between Raman spectroscopy readings of phosphate apatite (~960 cm-1), fluoridated apatite (~575 cm-1) and organic matrix (~1450 cm-1) levels and Diagnodent® readings at different stages of dental caries in extracted human teeth. The mean peak value of fluorescence in the carious area was recorded and teeth were divided in enamel caries, dentin caries and sound dental structure. After fluorescence readings, Raman spectroscopy was carried out on the same sites. The results showed significant difference (ANOVA, p<0.05) between the fluorescence readings for enamel (16.4 ± 2.3) and dentin (57.6 ± 23.7) on carious teeth. Raman peaks of enamel and dentin revealed that ~575 and ~960 cm-1 peaks were more intense in enamel caries. There was significant negative correlation (p<0.05) between the ~575 and ~960 cm-1 peaks and dentin caries. It may be concluded that the higher the fluorescence detected by Diagnodent the lower the peaks of phosphate apatite and fluoridated apatite. As the early diagnosis of caries is directly related to the identification of changes in the inorganic tooth components, Raman spectroscopy was more sensitive to variations of these components than Diagnodent.
authors: Biophotonics Center, Dental School, Federal Universityof Bahia, Salvador, BA, Brazil.
authors: Fabíola Bastos de
authors: FB
authors: Carvalho
authors: Artur Felipe Santos
authors: AF
authors: Barbosa
authors: Fátima Antonia Aparecida
authors: FA
authors: Zanin
authors: Aldo
authors: A
authors: Brugnera Júnior
authors: Landulfo
authors: L
authors: Silveira Júnior
authors: Antonio Luiz Barbosa
authors: AL
authors: Pinheiro
mesh_terms: D000704:Analysis of Variance; D001031:Apatites / Q000032:analysis; D003731:Dental Caries / Q000175:diagnosis*; D003743:Dental Enamel / Q000737:chemistry*; D003804:Dentin / Q000737:chemistry*; D017886:Durapatite / Q000032:analysis*; D005453:Fluorescence; D005470:Fluorometry; D006801:Humans; D054023:Lasers, Semiconductor; D009930:Organic Chemicals / Q000032:analysis; D013059:Spectrum Analysis, Raman; D018709:Statistics, Nonparametric; D014732:Vibration
chemical_list: D001031:Apatites; D009930:Organic Chemicals; D017886:Durapatite; C025105:fluorapatite