title: Chitosan-functionalized silk fibroin 3D scaffold for keratocyte culture.
abstract: The goal of this study was to evaluate the potential suitability of an artificial membrane composed of silk fibroin (SF) functionalized by different ratios of chitosan (CS) as a substrate for the stroma of the cornea. Keratocytes were cultured on translucent membranes made of SF and CS with different ratios. The biophysical properties of the silk fibroin and chitosan (SF/CS) membrane were examined. The SF/CS showed tensile strengths that increased as the CS concentration increased, but the physical and mechanical properties of chitosan-functionalized silk fibroin scaffolds weakened significantly compared with those of native corneas. The resulting cell scaffolds were evaluated using western blot in addition to light and electron microscopy. The cell attachment and proliferation on the scaffold were similar to those on a plastic plate. Keratocytes cultured in serum on SF/CS exhibited stellate morphology along with a marked increase in the expression of keratocan compared with identical cultures on tissue culture plastics. The biocompatibility was tested by transplanting the acellular membrane into rabbit corneal stromal pockets. There was no inflammatory complication detected at any time point on the macroscopic level. Taken together, these results indicate that SF/CS holds promise as a substrate for corneal reconstruction.
authors: Eye Hospital, The First Affiliated Hospital of Harbin Medical University, 23 Youzheng Street, Harbin, 150001, People's Republic of China.
authors: Linan
authors: L
authors: Guan
authors: Pei
authors: P
authors: Tian
authors: Hongyan
authors: H
authors: Ge
authors: Xianling
authors: X
authors: Tang
authors: Hong
authors: H
authors: Zhang
authors: Lingling
authors: L
authors: Du
authors: Ping
authors: P
authors: Liu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D049109:Cell Proliferation; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry / Q000494:pharmacology*; D003315:Cornea / Q000187:drug effects* / Q000601:surgery; D005136:Eye Proteins / Q000235:genetics / Q000378:metabolism; D005349:Fibroins / Q000737:chemistry / Q000494:pharmacology*; D015870:Gene Expression; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008422:Materials Testing; D011509:Proteoglycans / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D013718:Tensile Strength; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D005136:Eye Proteins; D011509:Proteoglycans; D005349:Fibroins; D048271:Chitosan
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