title: Antennapedia is involved in the development of thoracic legs and segmentation in the silkworm, Bombyx mori.
abstract: Homeotic genes, which are associated closely with body patterning of various species, specify segment identity. The Wedge eye-spot (Wes) is a new homeotic mutant located on the sixth linkage group. Homozygous Wes/Wes embryos are lethal and display a pair of antenna-like appendages under the mouthparts as well as fused thoracic segments. These mutants also exhibit a narrower eye-spot at the larval stage compared with the wild type. By positional cloning, we identified the candidate gene of the Wes locus, Bombyx mori Antennapedia (BmAntp). Two BmAntp transcripts were identified in the homozygote of the Wes mutant, including a normal form and an abnormal form with a 1570-bp insertion. Our data showed that the insertion element was a long interspersed nuclear element (LINE)-like transposon that destroyed the original open reading frame of BmAntp. Quantitative RT-PCR analysis showed that the expression levels of normal BmAntp transcripts were increased markedly in the Wes heterozygous larvae compared with the wild type. Furthermore, we performed RNAi of BmAntp and observed fused thoracic segments and defective thoracic legs in the developing embryos. Our results indicated that BmAntp is responsible for the Wes mutant and has an important role in determining the proper development of the thoracic segments. Our identification of a homeotic mutation in the silkworm is an important contribution to our understanding of the regulation of Hox genes at different levels of expression.
authors: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
authors: P
authors: P
authors: Chen
authors: X L
authors: XL
authors: Tong
authors: D D
authors: DD
authors: Li
authors: M Y
authors: MY
authors: Fu
authors: S Z
authors: SZ
authors: He
authors: H
authors: H
authors: Hu
authors: Z H
authors: ZH
authors: Xiang
authors: C
authors: C
authors: Lu
authors: F Y
authors: FY
authors: Dai
mesh_terms: D000825:Animal Structures / Q000254:growth & development* / Q000378:metabolism; D000818:Animals; D051943:Antennapedia Homeodomain Protein / Q000235:genetics / Q000378:metabolism*; D019521:Body Patterning; D012831:Bombyx / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D004251:DNA Transposable Elements; D018507:Gene Expression Regulation, Developmental; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D051943:Antennapedia Homeodomain Protein; D004251:DNA Transposable Elements; D019476:Insect Proteins
references: Nature. 2004 Oct 7;431(7009):653-9
references: 15470419
references: Adv Genet. 2011;76:55-91
references: 22099692
references: Genes Genet Syst. 1998 Dec;73(6):353-8
references: 10333566
references: Nat Rev Genet. 2009 Oct;10(10):691-703
references: 19763152
references: Dev Genes Evol. 2004 Jul;214(7):324-38
references: 15170569
references: Development. 2003 Nov;130(22):5445-55
references: 14507783
references: EMBO J. 1986 Dec 1;5(12):3327-34
references: 16453732
references: Development. 1992 Mar;114(3):555-63
references: 1352236
references: Genes Cells. 1996 Jun;1(6):555-68
references: 9078385
references: Cell. 2000 Jun 9;101(6):577-80
references: 10892643
references: Dev Biol. 2004 Mar 15;267(2):462-72
references: 15013806
references: Proc Natl Acad Sci U S A. 1994 Dec 6;91(25):12150-4
references: 7991598
references: Dev Biol. 1989 May;133(1):196-209
references: 2565268
references: FEBS J. 2005 Oct;272(20):5101-9
references: 16218944
references: Nature. 1987 Feb 26-Mar 4;325(6107):816-8
references: 3821869
references: Proc Natl Acad Sci U S A. 2005 Nov 8;102(45):16303-8
references: 16263926
references: Genetics. 1986 Nov;114(3):919-42
references: 3098627
references: Heredity (Edinb). 2008 May;100(5):533-40
references: 18364737
references: Cell. 2006 Jun 30;125(7):1387-99
references: 16814723
references: Nature. 1997 Aug 14;388(6643):682-6
references: 9262403
references: Evol Dev. 2002 Nov-Dec;4(6):459-99
references: 12492146
references: Dev Biol. 1981 Jan 15;81(1):51-64
references: 6780397
references: Cell. 1986 Oct 10;47(1):113-22
references: 3093083
references: Dev Genes Evol. 2009 Oct;219(9-10):437-44
references: 19908062
references: Nature. 1995 Aug 10;376(6540):479-85
references: 7637779
references: Cell. 1992 Jan 24;68(2):283-302
references: 1346368
references: Proc Natl Acad Sci U S A. 2008 Sep 9;105(36):13439-44
references: 18755899
references: Dev Biol. 2009 Apr 15;328(2):403-9
references: 19389347
references: Development. 2008 May;135(9):1669-79
references: 18367556
references: J Comput Biol. 2000 Feb-Apr;7(1-2):203-14
references: 10890397
references: Proc Natl Acad Sci U S A. 2012 Mar 27;109(13):4921-6
references: 22421434
references: Curr Biol. 1999 Feb 11;9(3):109-15
references: 10021383
references: BMC Genomics. 2009 Aug 21;10:389
references: 19698097
references: Nucleic Acids Res. 2010 Jan;38(Database issue):D453-6
references: 19793867
references: Development. 2000 Apr;127(8):1617-26
references: 10725238