title: The effects of Nanos2 on Boule and Stra8 in male germline stem cells (mGSCs).
abstract: The mitosis-meiosis switch is a key event in the differentiation of germ cells. Meiosis is important in development biology, however, it has not been clear what is the regulation mechanism in mammals. Our previous study showed that Boule could activate Stra8 directly and result in the meiosis initiation of dairy goat male germline stem cells (mGSCs). Nanos2, a RNA-binding protein, plays critical roles in the suppression of meiosis by preventing Stra8 expression and maintain the male germ cell development. The main purpose of this study was to explore whether Nanos2 represses Stra8 transcription through Boule or not. We found ectopic over-expression of Nanos2 in GC-1 and mGSCs down-regulated Stra8 transcription and translation, and Boule expression was not affected. It was in consistent with our expectation that RA could up-regulate Boule and Stra8 expression, but down-regulate Nanos2 expression in mGSCs. In dairy goat, the expression levels of Boule and Stra8 would rise with the increase of age, but the expression level of Nanos2 in 90 dpp and adult testis had not shown a clear change. In conclusion, Nanos2 represses Stra8 expression but not through Boule in dairy goat mGSCs.
authors: Key Lab for Animal Biotechnology of Agriculture Ministry, Shaanxi Centre of Stem Cells Engineering & Technology, College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi, China.
authors: Mingzhao
authors: M
authors: Li
authors: Meng
authors: M
authors: Yu
authors: Haijing
authors: H
authors: Zhu
authors: Wencong
authors: W
authors: Song
authors: Jinlian
authors: J
authors: Hua
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D005854:Germ Cells / Q000378:metabolism*; D006041:Goats; D008297:Male; D008540:Meiosis; D052247:Nitric Oxide Synthase Type II / Q000235:genetics* / Q000378:metabolism; D014176:Protein Biosynthesis; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction; D013234:Stem Cells / Q000378:metabolism*; D013737:Testis / Q000378:metabolism; D014158:Transcription, Genetic; D014212:Tretinoin / Q000494:pharmacology
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D016601:RNA-Binding Proteins; D014212:Tretinoin; D052247:Nitric Oxide Synthase Type II
references: Proc Natl Acad Sci U S A. 2008 Sep 30;105(39):14976-80
references: 18799751
references: Mol Hum Reprod. 2012 Nov;18(11):523-34
references: 22899867
references: Endocrinology. 2007 Oct;148(10):4560-7
references: 17584971
references: Science. 2006 Apr 28;312(5773):596-600
references: 16574820
references: Anim Reprod Sci. 2013 Feb;137(1-2):45-52
references: 23290695
references: J Androl. 2000 Nov-Dec;21(6):776-98
references: 11105904
references: PLoS One. 2011;6(6):e20249
references: 21674038
references: Reproduction. 2001 Mar;121(3):347-54
references: 11226060
references: Science. 2003 Aug 29;301(5637):1239-41
references: 12947200
references: Cell Reprogram. 2011 Apr;13(2):133-44
references: 21473690
references: Dev Cell. 2010 Oct 19;19(4):612-24
references: 20951351
references: Development. 1999 Nov;126(21):4861-71
references: 10518502
references: Gene Expr Patterns. 2006 Jun;6(5):572-7
references: 16530490
references: Hum Mol Genet. 2010 Jun 15;19(12):2360-9
references: 20335278
references: PLoS One. 2012;7(3):e33558
references: 22448252
references: Stem Cells. 2012 Feb;30(2):280-91
references: 22102605
references: Hum Mol Genet. 2003 Jan 15;12(2):169-75
references: 12499397
references: Genes Dev. 2008 Feb 15;22(4):430-5
references: 18281459
references: J Assist Reprod Genet. 2002 Nov;19(11):531-5
references: 12489526
references: Theriogenology. 2012 Jun;77(9):1939-50
references: 22444555
references: J Cell Biochem. 2013 Feb;114(2):294-302
references: 22930651
references: Nat Protoc. 2010 May;5(5):921-8
references: 20431537
references: Cell Cycle. 2008 Mar 1;7(5):656-64
references: 18256537
references: Cell Prolif. 2013 Apr;46(2):223-31
references: 23510477
references: Endocrinology. 2012 May;153(5):2466-73
references: 22396454
references: Cell Stem Cell. 2009 Jul 2;5(1):87-96
references: 19570517
references: Science. 2009 Sep 11;325(5946):1394-8
references: 19745153
references: Exp Cell Res. 1992 Aug;201(2):417-35
references: 1322317
references: J Cell Biol. 1996 Oct;135(2):469-77
references: 8896602
references: Hum Reprod. 2005 Mar;20(3):782-8
references: 15591084