title: Profiling the indole alkaloids in yohimbe bark with ultra-performance liquid chromatography coupled with ion mobility quadrupole time-of-flight mass spectrometry.
abstract: An ultra-performance liquid chromatography/ion mobility quadrupole time-of-flight mass spectrometry (UPLC/IM-QTOF-MS) method was developed for profiling the indole alkaloids in yohimbe bark. Many indole alkaloids with the yohimbine or ajmalicine core structure, plus methylated, oxidized and reduced species, were characterized. Common fragments and mass differences are described. It was shown that the use of IMS could provide another molecular descriptor, i.e. molecular shape by rotationally averaged collision cross-section; this is of great value for identification of constituents when reference materials are usually not available. Using the combination of high resolution (~40000) accurate mass measurement with time-aligned parallel (TAP) fragmentation, MS(E) (where E represents collision energy), ion mobility mass spectrometry (IMS) and UPLC chromatography, a total 55 indole alkaloids were characterized and a few new indole alkaloids are reported for the first time.
authors: U.S. Department of Agriculture, Agricultural Research Service, Beltsville Human Nutrition Research Center, Food Composition and Methods Development Laboratory, Beltsville, MD, USA.
authors: Jianghao
authors: J
authors: Sun
authors: Andrew
authors: A
authors: Baker
authors: Pei
authors: P
authors: Chen
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D026121:Indole Alkaloids / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D032068:Pausinystalia / Q000737:chemistry*; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D015016:Yohimbine / Q000737:chemistry
chemical_list: D026121:Indole Alkaloids; D010936:Plant Extracts; D015016:Yohimbine