title: Complex structure of OspI and Ubc13: the molecular basis of Ubc13 deamidation and convergence of bacterial and host E2 recognition.
abstract: Ubc13 is an important ubiquitin-conjugating (E2) enzyme in the NF-κB signaling pathway. The Shigella effector OspI targets Ubc13 and deamidates Gln100 of Ubc13 to a glutamic acid residue, leading to the inhibition of host inflammatory responses. Here we report the crystal structure of the OspI-Ubc13 complex at 2.3 Å resolution. The structure reveals that OspI uses two differently charged regions to extensively interact with the α1 helix, L1 loop and L2 loop of Ubc13. The Gln100 residue is bound within the hydrophilic catalytic pocket of OspI. A comparison between Ubc13-bound and wild-type free OspI structures revealed that Ubc13 binding induces notable structural reassembly of the catalytic pocket, suggesting that substrate binding might be involved in the catalysis of OspI. The OspI-binding sites in Ubc13 largely overlap with the binding residues for host ubiquitin E3 ligases and a deubiquitinating enzyme, which suggests that the bacterial effector and host proteins exploit the same surface on Ubc13 for specific recognition. Biochemical results indicate that both of the differently charged regions in OspI are important for the interaction with Ubc13, and the specificity determinants in Ubc13 for OspI recognition reside in the distinct residues in the α1 helix and L2 region. Our study reveals the molecular basis of Ubc13 deamidation by OspI, as well as a convergence of E2 recognition by bacterial and host proteins.
authors: Life Sciences Institute, Zhejiang University, Hangzhou, Zhejiang, China.
authors: Panhan
authors: P
authors: Fu
authors: Xiaoqing
authors: X
authors: Zhang
authors: Mengmeng
authors: M
authors: Jin
authors: Li
authors: L
authors: Xu
authors: Chong
authors: C
authors: Wang
authors: Zongping
authors: Z
authors: Xia
authors: Yongqun
authors: Y
authors: Zhu
mesh_terms: D000581:Amidohydrolases / Q000737:chemistry* / Q000378:metabolism*; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism*; D058947:Bacterial Secretion Systems; D001665:Binding Sites / Q000235:genetics; D018360:Crystallography, X-Ray; D057809:HEK293 Cells; D006801:Humans; D007249:Inflammation / Q000276:immunology / Q000382:microbiology; D046912:Multiprotein Complexes / Q000737:chemistry / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D011485:Protein Binding / Q000235:genetics; D016415:Sequence Alignment; D012763:Shigella flexneri / Q000276:immunology / Q000378:metabolism*; D048029:TNF Receptor-Associated Factor 6 / Q000378:metabolism; D044763:Ubiquitin-Conjugating Enzymes / Q000737:chemistry* / Q000378:metabolism*; D054875:Ubiquitination
chemical_list: D001426:Bacterial Proteins; D058947:Bacterial Secretion Systems; D046912:Multiprotein Complexes; D016328:NF-kappa B; D048029:TNF Receptor-Associated Factor 6; C471607:UBE2N protein, human; D044763:Ubiquitin-Conjugating Enzymes; D000581:Amidohydrolases
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