title: Use of "MGE enhancers" for labeling and selection of embryonic stem cell-derived medial ganglionic eminence (MGE) progenitors and neurons.
abstract: The medial ganglionic eminence (MGE) is an embryonic forebrain structure that generates the majority of cortical interneurons. MGE transplantation into specific regions of the postnatal central nervous system modifies circuit function and improves deficits in mouse models of epilepsy, Parkinson's disease, pain, and phencyclidine-induced cognitive deficits. Herein, we describe approaches to generate MGE-like progenitor cells from mouse embryonic stem (ES) cells. Using a modified embryoid body method, we provided gene expression evidence that mouse ES-derived Lhx6(+) cells closely resemble immature interneurons generated from authentic MGE-derived Lhx6(+) cells. We hypothesized that enhancers that are active in the mouse MGE would be useful tools in detecting when ES cells differentiate into MGE cells. Here we demonstrate the utility of enhancer elements [422 (DlxI12b), Lhx6, 692, 1056, and 1538] as tools to mark MGE-like cells in ES cell differentiation experiments. We found that enhancers DlxI12b, 692, and 1538 are active in Lhx6-GFP(+) cells, while enhancer 1056 is active in Olig2(+) cells. These data demonstrate unique techniques to follow and purify MGE-like derivatives from ES cells, including GABAergic cortical interneurons and oligodendrocytes, for use in stem cell-based therapeutic assays and treatments.
authors: Department of Psychiatry and the Nina Ireland Laboratory of Developmental Neurobiology, University of California San Francisco, San Francisco, CA, USA. nyc600@gmail.com
authors: Ying-Jiun J
authors: YJ
authors: Chen
authors: Daniel
authors: D
authors: Vogt
authors: Yanling
authors: Y
authors: Wang
authors: Axel
authors: A
authors: Visel
authors: Shanni N
authors: SN
authors: Silberberg
authors: Cory R
authors: CR
authors: Nicholas
authors: Teruko
authors: T
authors: Danjo
authors: Joshua L
authors: JL
authors: Pollack
authors: Len A
authors: LA
authors: Pennacchio
authors: Stewart
authors: S
authors: Anderson
authors: Yoshiki
authors: Y
authors: Sasai
authors: Scott C
authors: SC
authors: Baraban
authors: Arnold R
authors: AR
authors: Kriegstein
authors: Arturo
authors: A
authors: Alvarez-Buylla
authors: John L R
authors: JL
authors: Rubenstein
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D002469:Cell Separation; D002478:Cells, Cultured; D058732:Embryoid Bodies / Q000502:physiology* / Q000637:transplantation; D004742:Enhancer Elements, Genetic; D005260:Female; D005434:Flow Cytometry; D059330:GABAergic Neurons / Q000378:metabolism; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D057809:HEK293 Cells; D006801:Humans; D008164:Luminescent Proteins / Q000096:biosynthesis / Q000235:genetics; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D058953:Neural Stem Cells / Q000502:physiology*; D011401:Promoter Regions, Genetic; D016548:Prosencephalon / Q000166:cytology*; D013194:Staining and Labeling; D059467:Transcriptome; D014161:Transduction, Genetic; D000097573:Red Fluorescent Protein
chemical_list: D015415:Biomarkers; D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein
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