title: Purification, characterization, and chemical modification of neurotoxic peptide from Daboia russelii snake venom of India.
abstract: Comprehensive knowledge of venom composition is very important for effective management of snake envenomation and antivenom preparation. Daboia russelii venom from the eastern region of India is the most neurotoxic among the four venom samples investigated. From the eastern D. russelii venom sample, neurotoxic peptide has been purified by combined method of ion exchange gel permeation chromatography and reversed phase high performance liquid chromatography. Molecular weight of Daboia neurotoxin III (DNTx-III) found to be 6,849 Da (as measured on matrix-assisted laser desorption/ionisation-time of flight mass spectrometer), and N-terminal amino acid sequences is I K C F I T P D U T S Q A. Approximate LD50 dosage was 0.24 mg/kg body weight. It produced concentration- and time-dependent inhibition of indirectly stimulated twitches of Rana hexadactyla sciatic nerve gastrocnemius muscle preparations. Chemical modification of DNTx-III tryptophan residue(s) reduced the twitch height inhibition property of toxin, signifying the importance of tryptophan residues for the neurotoxic function. This type of neurotoxic peptide is unique to east Indian regional D. russelii venom.
authors: Department of Studies in Biochemistry, University of Mysore, Mysore, 570 006, India.
authors: Madhukumar
authors: M
authors: Venkatesh
authors: Nijaguna
authors: N
authors: Prasad
authors: Tej
authors: T
authors: Sing
authors: Veerabasappa
authors: V
authors: Gowda
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002645:Chickens; D005260:Female; D006801:Humans; D007194:India; D007928:Lethal Dose 50; D008297:Male; D051379:Mice; D008970:Molecular Weight; D009469:Neuromuscular Junction / Q000187:drug effects; D009498:Neurotoxins / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity; D011898:Ranidae; D017840:Daboia; D012909:Snake Bites / Q000209:etiology / Q000628:therapy; D014364:Tryptophan / Q000737:chemistry; D014757:Viper Venoms / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity
chemical_list: D009498:Neurotoxins; D010455:Peptides; D014757:Viper Venoms; D014364:Tryptophan