title: Molecular interaction of a new antibacterial polymer with a supported lipid bilayer measured by an in situ label-free optical technique.
abstract: The interaction of the antibacterial polymer-branched poly(ethylene imine) substituted with quaternary ammonium groups, PEO and alkyl chains, PEI25QI5J5A815-with a solid supported lipid bilayer was investigated using surface sensitive optical waveguide spectroscopy. The analysis of the optogeometrical parameters was extended developing a new composite layer model in which the structural and optical anisotropy of the molecular layers was taken into consideration. Following in situ the change of optical birefringence we were able to determine the composition of the lipid/polymer surface layer as well as the displacement of lipid bilayer by the antibacterial polymer without using additional labeling. Comparative assessment of the data of layer thickness and optical anisotropy helps to reveal the molecular mechanism of antibacterial effect of the polymer investigated.
authors: MTA TTK MFA Institute for Technical Physics and Materials Science, Research Centre for Natural Sciences, Budapest, Konkoly Thege u. 29-33 H-1121, Hungary. keul@dwi.rwth-aachen.de.
authors: Robert
authors: R
authors: Horvath
authors: Balázs
authors: B
authors: Kobzi
authors: Helmut
authors: H
authors: Keul
authors: Martin
authors: M
authors: Moeller
authors: Eva
authors: E
authors: Kiss
mesh_terms: D000327:Adsorption; D000478:Alkylation; D064751:Ammonium Compounds / Q000737:chemistry*; D016880:Anisotropy; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D008027:Light; D008051:Lipid Bilayers / Q000737:chemistry*; D008956:Models, Chemical; D008958:Models, Molecular; D010713:Phosphatidylcholines / Q000737:chemistry*; D011094:Polyethyleneimine / Q000737:chemistry*; D013057:Spectrum Analysis
chemical_list: D064751:Ammonium Compounds; D000900:Anti-Bacterial Agents; D008051:Lipid Bilayers; D010713:Phosphatidylcholines; D011094:Polyethyleneimine; C028694:1-palmitoyl-2-oleoylphosphatidylcholine
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