title: Structural determination and NMR characterization of a bacterial exopolysaccharide.
abstract: A strain of Bacillus licheniformis with high exopolysaccharide (EPS) production ability was isolated and identified. A new type of EPS was isolated from the strain fermentation and its structural characteristics were investigated and elucidated by partial and total acid hydrolysis, Fourier transform infrared, and (1)H and (13)C NMR spectroscopy including 2D (1)H, COSY, NOESY, XHCOR and HMBC experiments. Based on obtained data, the EPS was found to be a levan composed of linear chains of (2→6)-linked β-d-fructofuranosyl residues with connections β (2→6).
authors: Biochemistry Laboratory, Faculty of Sciences of Sfax, PB 802, 3018 Sfax, Tunisia. imenbiologie@yahoo.fr
authors: Imen
authors: I
authors: Dahech
authors: Jawhar
authors: J
authors: Fakhfakh
authors: Mohamed
authors: M
authors: Damak
authors: Hafedh
authors: H
authors: Belghith
authors: Hafedh
authors: H
authors: Mejdoub
authors: Karima Srih
authors: KS
authors: Belghith
mesh_terms: D001407:Bacillus / Q000737:chemistry*; D005285:Fermentation; D005630:Fructans / Q000737:chemistry* / Q000302:isolation & purification; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000302:isolation & purification; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D005630:Fructans; D011135:Polysaccharides, Bacterial; C072599:levan