title: Ascaris lumbricoides infection in urban schoolchildren: specific IgE and IL-10 production.
abstract: BACKGROUND
  Helminth infections and allergies are diseases with intense Th2 lymphocytes participation and characterised by a high IgE and Interleukin-(IL) IL-4, IL-5 production and eosinophilia. However, helminths also induce IL-10 production, which may alter the outcome of allergic diseases in infected patients.
  
  
  OBJECTIVE
  This experimental study analyses the relationship between IL-10 production by cell culture from geohelminth infected and non-infected children and specific IgE to Ascaris lumbricoides (Asc) or Blomia tropicalis (BT).
  
  
  METHODS
  IL-10 content in supernatant from peripheral blood mononuclear cell culture from nine helminth infected and eleven non-infected patients was determined by ELISA after in vitro stimulation with Asc or BT extracts.
  
  
  RESULTS
  A positive association was observed between total IgE levels and anti-Ascaris and anti-Blomia tropicalis specific IgE, independent of infection status. For both helminth-infected and non-infected groups, there was no difference in IL-10 production in response to Asc extract, even though anti-Ascaris IgE levels were higher in the latter group. In response to BT stimulus, a lower production of IL-10 by the geohelminth-infected group was observed, but with no relationship between IL-10 production and specific IgE to BT.
  
  
  CONCLUSIONS
  The results suggest that anti-Ascaris IgE in non-infected patients may be associated to a resistance to parasites. Levels of specific IgE to parasite antigens or B. tropicalis allergen were not impaired by IL-10 production in children from an urban area in which geohelminthiasis is endemic.
authors: Keizo Asami Laboratory of Immunopathology, Federal University of Pernambuco, Recife, Brazil.
authors: V
authors: V
authors: Souza
authors: Research Center for Allergy and Clinical Immunology, Federal University of Pernambuco, Recife, Brazil.
authors: D
authors: D
authors: Medeiros
authors: Keizo Asami Laboratory of Immunopathology, Federal University of Pernambuco, Recife, Brazil.
authors: I
authors: I
authors: Sales
authors: Keizo Asami Laboratory of Immunopathology, Federal University of Pernambuco, Recife, Brazil.
authors: V
authors: V
authors: Costa
authors: Research Center for Allergy and Clinical Immunology, Federal University of Pernambuco, Recife, Brazil.
authors: A
authors: A
authors: Silva
authors: Research Center for Allergy and Clinical Immunology, Federal University of Pernambuco, Recife, Brazil.
authors: J
authors: J
authors: Rizzo
authors: Division of Allergy, Clinical Immunology and Rheumatology, Federal University of São Paulo, São Paulo, Brazil.
authors: D
authors: D
authors: Sole
authors: Research Center for Allergy and Clinical Immunology, Federal University of Pernambuco, Recife, Brazil. Electronic address: emanuel.sarinho@gmail.com.
authors: E
authors: E
authors: Sarinho
mesh_terms: D040041:Acaridae / Q000276:immunology; D000485:Allergens / Q000276:immunology; D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology; D001196:Ascariasis / Q000276:immunology*; D017164:Ascaris lumbricoides / Q000276:immunology*; D002478:Cells, Cultured; D002648:Child; D005260:Female; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology*; D007073:Immunoglobulin E / Q000097:blood*; D019476:Insect Proteins / Q000276:immunology; D016753:Interleukin-10 / Q000097:blood*; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000469:parasitology; D008297:Male; D011153:Population; D012882:Skin Tests; D014505:Urban Population
chemical_list: D000485:Allergens; D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D019476:Insect Proteins; D016753:Interleukin-10; D007073:Immunoglobulin E
keywords: Ascaris lumbricoides; Blomia tropicalis; Immunoglobulin E; Intelerkin-10; Schoolchildren; Urban area