title: "Zoom-ln"--A targeted database search for identification of glycation modifications analyzed by untargeted tandem mass spectrometry.
abstract: Post-translational modifications (PTMs) are very important to biological function, however their identification and characterization is technically challenging. In this study, we have identified glycation modifications by nano LC-MSE, a data independent acquisition work flow, followed by database search using the Protein Lynx Global Server (PLGSJ). PLGS search with a complete human protein database hardly identified glycation modifications in a glycated human serum albumin (HSA), which was detected to be glycated by western blotting with advanced glycation end products (AGE) antibody and fluorescence spectroscopy. To overcome this difficulty, "Zoom-In" approach, a targeted database search was used to identify glycation modifications in a glycated HSA, which were further manually validated. This approach was useful for identification of glycation modifications from untargeted tandem mass spectrometryworkflow such as MSE, but may require the development of a new algorithm or an upgrade of the existing software.
authors: Proteomics Facility, Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune 411008, India.
authors: Hemangi S
authors: HS
authors: Bhonsle
authors: Arvind M
authors: AM
authors: Korwar
authors: Suresh K
authors: SK
authors: Kesavan
authors: Santosh D
authors: SD
authors: Bhosale
authors: Sneha B
authors: SB
authors: Bansode
authors: Mahesh J
authors: MJ
authors: Kulkarni
mesh_terms: D000595:Amino Acid Sequence; D015153:Blotting, Western; D030562:Databases, Protein; D017127:Glycation End Products, Advanced / Q000032:analysis*; D006020:Glycopeptides / Q000737:chemistry / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D008969:Molecular Sequence Data; D011499:Protein Processing, Post-Translational; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D012709:Serum Albumin / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D017127:Glycation End Products, Advanced; D006020:Glycopeptides; D011506:Proteins; D012709:Serum Albumin