title: Ultrasensitive detection of proteins on Western blots with semiconducting polymer dots.
abstract: Ultrasensitive fluorescence imaging of proteins on western blots using a bright, compact, and orange-emitting semiconducting polymer dot (CN-PPV) is demonstrated. A detection limit at the single-picogram level in dot blots is achieved; with conventional western blotting, 50 pg of transferrin and trypsin inhibitor after sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transfer onto a polyvinylidene fluoride membrane are detected. This method does not require any additional equipment or time compared with the conventional procedure with traditional fluorescent probes.
authors: Department of Chemistry, University of Washington, Seattle, WA 98195, USA.
authors: Fangmao
authors: F
authors: Ye
authors: Polina B
authors: PB
authors: Smith
authors: Changfeng
authors: C
authors: Wu
authors: Daniel T
authors: DT
authors: Chiu
mesh_terms: D015153:Blotting, Western / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D005456:Fluorescent Dyes / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry; D011506:Proteins / Q000032:analysis*; D012666:Semiconductors / Q000592:standards*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D005465:Fluorocarbon Polymers
chemical_list: D005456:Fluorescent Dyes; D011108:Polymers; D011145:Polyvinyls; D011506:Proteins; D012967:Sodium Dodecyl Sulfate; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers
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