title: Small changes in ambient temperature affect alternative splicing in Arabidopsis thaliana.
abstract: Alternative splicing (AS) gives rise to multiple mRNA isoforms from the same gene, providing possibilities to regulate gene expression beyond the level of transcription. In a recent paper in Nucleic Acids Research we used a high resolution RT-PCR based panel to study changes in AS patterns in plants with altered levels of an hnRNP-like RNA-binding protein in Arabidopsis thaliana. Furthermore, we detected significant changes in AS patterns between different Arabidopsis ecotypes. Here we investigated how small changes in ambient temperature affect AS. We found significant changes in AS for 12 of 28 investigated events (43%) upon transfer of Arabidopsis plants from 20°C to 16°C and for 6 of the 28 investigated events (21%) upon transfer from 20°C to 24°C.
authors: Molecular Cell Physiology; Bielefeld University; Bielefeld, Germany.
authors: Corinna
authors: C
authors: Streitner
authors: Craig G
authors: CG
authors: Simpson
authors: Paul
authors: P
authors: Shaw
authors: Selahattin
authors: S
authors: Danisman
authors: John W S
authors: JW
authors: Brown
authors: Dorothee
authors: D
authors: Staiger
mesh_terms: D017398:Alternative Splicing; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D017343:Genes, Plant; D020033:Protein Isoforms / Q000378:metabolism; D013696:Temperature
chemical_list: D020033:Protein Isoforms
keywords: RNA-binding proteins; alternative splicing; posttranscriptional regulation; temperature
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