title: Genetic variation in the HN and SH genes of mumps viruses: a comparison of strains from mumps cases with and without neurological symptoms.
abstract: BACKGROUND
  It is known that mumps virus (MuV) strains may vary in their neurovirulent capacity, and certain MuV strains may be highly neurotropic. In animal models and epidemiological studies, mutations at specific amino acids (aa) have been proposed to be associated with neurovirulence. To assess whether these genetic variations can be observed in clinical samples from patients and if they correlate with neurovirulence as determined by clinical symptoms, 39 mumps patients with or without neurological symptoms were investigated.
  
  
  RESULTS
  Respiratory samples, oral fluids, throat swabs, and neurological and cerebrospinal fluid samples were tested by RT-PCR and products sequenced. Sequences of the entire small hydrophobic (SH) gene and the partial hemagglutinin-neuraminidase (HN) gene were compared.
  
  
  CONCLUSIONS
  The results showed there was no significant difference between the samples of the two groups of patients at the aa sites in either the HN protein or the SH protein, which have previously been hypothesized to be associated with neurovirulence or antigenicity. The occurrence of neurological symptoms of mumps does not appear to be due to a single point mutation in either the HN or SH gene.
authors: National Institute of Viral Disease Control and Prevention, Chinese Centre for Disease Control and Prevention, Beijing, People's Republic of China.
authors: Aili
authors: A
authors: Cui
authors: David W G
authors: DW
authors: Brown
authors: Wenbo
authors: W
authors: Xu
authors: Li
authors: L
authors: Jin
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D005838:Genotype; D015707:HN Protein / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D009107:Mumps / Q000821:virology*; D009109:Mumps virus / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity; D054730:Protein Interaction Domains and Motifs; D016415:Sequence Alignment; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*; D014774:Virulence / Q000235:genetics
chemical_list: D015707:HN Protein; D014764:Viral Proteins; C067386:small hydrophobic protein, Mumps virus
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