title: [Research of the whole grain-soybean compound package to regulate the cholesterol metabolism by SREBP-2, LDLR and visfatin].
abstract: OBJECTIVE
  To discuss the possible mechanism of the whole grain-soybean compound package on dyslipidaemia rats.
  
  
  METHODS
  44 SD rats were randomly divided into four groups: the hyperlipidaemic group, the rice-flour group, the whole grain-soybean compound package group and the negative control group by lipid profiles, fed with corresponding feed for eight weeks. Serum total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) were measured before and after the test. At the end of the experiment, blood samples were taken from the femoral artery, the rat organs were collected and weighted. Serum Visfatin levels and SREBP-2 mRNA and LDLR mRNA in the liver were measured.
  
  
  RESULTS
  Compared with the hyperlipidaemic group and rice-flour group, the body weight, serum TC, TG and LDL-C of whole grain-soybean compound package group were significantly decreased (P < 0.05), HDL-C was significantly increased (P < 0.05). Visfatin levels were significantly decreased (P < 0.05). Gene expressions of SREBP-2 and LDLR were significantly increased (P < 0.05). Gene expression of Visfatin in whole grain-soybean compound package group was significantly lower than that in hyperlipidaemia group and rice-flour group (P < 0.05).
  
  
  CONCLUSIONS
  Whole grain-soybean compound package can improve the serum lipid profiles and Visfatin of rats fed with a high fat diet. The possible mechanism is that the whole grain-soybean compound package can activate the expression of SREBP-2, LDLR and Visfatin. And then enhance the expressing activity of regulate the cholesterol metabolism by SREBP-2, LDLR and Visfatin. Ultimately, to reduce the level of rats' cholesterol, and then ameliorate the dyslipidaemia of rats.
authors: Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, Nanjing 210009, China. liuwen2185@163.com
authors: Wen
authors: W
authors: Liu
authors: Chengkai
authors: C
authors: Zhai
authors: Xiaoqiang
authors: X
authors: Zhang
authors: Hong
authors: H
authors: Zhang
authors: Mingxia
authors: M
authors: Jiang
authors: Siyu
authors: S
authors: Zhou
authors: Yang
authors: Y
authors: Liu
authors: Na
authors: N
authors: Zhao
authors: Junhong
authors: J
authors: Zhao
mesh_terms: D000821:Animal Feed; D000818:Animals; D002523:Edible Grain; D006949:Hyperlipidemias / Q000517:prevention & control*; D050356:Lipid Metabolism; D008055:Lipids / Q000097:blood; D008099:Liver / Q000378:metabolism; D008297:Male; D054409:Nicotinamide Phosphoribosyltransferase / Q000097:blood; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011973:Receptors, LDL / Q000235:genetics / Q000378:metabolism*; D013025:Glycine max; D051782:Sterol Regulatory Element Binding Protein 2 / Q000235:genetics / Q000378:metabolism*
chemical_list: D008055:Lipids; D012333:RNA, Messenger; D011973:Receptors, LDL; D051782:Sterol Regulatory Element Binding Protein 2; D054409:Nicotinamide Phosphoribosyltransferase