title: The DEAH-box helicase DHX36 mediates dendritic localization of the neuronal precursor-microRNA-134.
abstract: Specific microRNAs (miRNAs), including miR-134, localize to neuronal dendrites, where they control synaptic protein synthesis and plasticity. However, the mechanism of miRNA transport is unknown. We found that the neuronal precursor-miRNA-134 (pre-miR-134) accumulates in dendrites of hippocampal neurons and at synapses in vivo. Dendritic localization of pre-miR-134 is mediated by the DEAH-box helicase DHX36, which directly associates with the pre-miR-134 terminal loop. DHX36 function is required for miR-134-dependent inhibition of target gene expression and the control of dendritic spine size. Dendritically localized pre-miR-134 could provide a local source of miR-134 that can be mobilized in an activity-dependent manner during plasticity.
authors: Institut für Physiologische Chemie, Biochemisch-Pharmakologisches Centrum Marburg, Philipps-Universität Marburg, 35032 Marburg, Germany.
authors: Silvia
authors: S
authors: Bicker
authors: Sharof
authors: S
authors: Khudayberdiev
authors: Kerstin
authors: K
authors: Weiß
authors: Kathleen
authors: K
authors: Zocher
authors: Stefan
authors: S
authors: Baumeister
authors: Gerhard
authors: G
authors: Schratt
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D053487:DEAD-box RNA Helicases / Q000235:genetics / Q000378:metabolism*; D003712:Dendrites / Q000201:enzymology*; D049229:Dendritic Spines / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D006624:Hippocampus / Q000378:metabolism; D035683:MicroRNAs / Q000378:metabolism*; D051381:Rats; D013574:Synaptosomes / Q000378:metabolism
chemical_list: C581995:MIRN134 microRNA, rat; D035683:MicroRNAs; C581994:DHX36 protein, rat; D053487:DEAD-box RNA Helicases
keywords: DHX36; dendritic transport; precursor-microRNA; protein synthesis; synaptic plasticity
references: EMBO J. 2009 Mar 18;28(6):697-710
references: 19197241
references: Mol Cell. 2007 May 25;26(4):523-37
references: 17531811
references: Cell. 2008 Oct 31;135(3):422-35
references: 18984155
references: Nat Cell Biol. 2009 Jun;11(6):705-16
references: 19465924
references: Cell. 2009 Feb 20;136(4):719-30
references: 19239891
references: J Neurochem. 2005 Aug;94(4):896-905
references: 16092937
references: Mol Cell. 2004 Jan 16;13(1):101-11
references: 14731398
references: Neuron. 2009 Sep 24;63(6):803-17
references: 19778509
references: Nature. 2010 Aug 26;466(7310):1105-9
references: 20622856
references: Nature. 2006 Jan 19;439(7074):283-9
references: 16421561
references: Mol Cell. 2011 Jun 10;42(5):673-88
references: 21658607
references: Front Neural Circuits. 2010 Jan 12;3:16
references: 20126250
references: Genes Dev. 2010 Jun 1;24(11):1086-92
references: 20516194
references: J Biol Chem. 2005 Nov 18;280(46):38117-20
references: 16150737
references: Immunity. 2011 Jun 24;34(6):866-78
references: 21703541
references: EMBO Rep. 2007 Nov;8(11):1052-60
references: 17932509
references: Results Probl Cell Differ. 2001;34:129-38
references: 11288672
references: Methods. 2007 Oct;43(2):105-9
references: 17889796
references: Nat Rev Neurosci. 2007 Oct;8(10):776-89
references: 17848965
references: Cell. 2006 Jan 13;124(1):191-205
references: 16413491
references: J Neurosci. 2006 Jul 5;26(27):7143-6
references: 16822969
references: Nat Rev Mol Cell Biol. 2005 May;6(5):376-85
references: 15852042
references: Neuron. 2009 Dec 24;64(6):871-84
references: 20064393
references: Science. 2007 Jan 5;315(5808):97-100
references: 17204650
references: J Neurosci. 2004 Aug 18;24(33):7366-77
references: 15317862
references: J Neurosci. 2006 Dec 20;26(51):13390-9
references: 17182790
references: Nat Rev Genet. 2010 Sep;11(9):597-610
references: 20661255
references: J Biol Chem. 2008 Dec 12;283(50):35186-98
references: 18854321
references: Mol Cell. 2008 Oct 24;32(2):276-84
references: 18951094
references: Neuron. 2010 Feb 11;65(3):373-84
references: 20159450
references: Cell. 2009 Aug 21;138(4):696-708
references: 19703396