title: Nanoscale patterning of membrane-bound proteins formed through curvature-induced partitioning of phase-specific receptor lipids.
abstract: This work describes a technique for forming high-density arrays and patterns of membrane-bound proteins through binding to a curvature-organized compositional pattern of metal-chelating lipids (Cu(2+)-DOIDA or Cu(2+)-DSIDA). In this bottom-up approach, the underlying support is an e-beam formed, square lattice pattern of hemispheres. This curvature pattern sorts Cu(2+)-DOIDA to the 200 nm hemispherical lattice sites of a 600 nm × 600 nm unit cell in Ld - Lo phase separated lipid multibilayers. Binding of histidine-tagged green fluorescent protein (His-GFP) creates a high density array of His-GFP-bound pixels localized to the square lattice sites. In comparison, the negative pixel pattern is created by sorting Cu(2+)-DSIDA in Ld - Lβ' phase separated lipid multibilayers to the flat grid between the lattice sites followed by binding to His-GFP. Lattice defects in the His-GFP pattern lead to interesting features such as pattern circularity. We also observe defect-free arrays of His-GFP that demonstrate perfect arrays can be formed by this method suggesting the possibility of using this approach for the localization of various active molecules to form protein, DNA, or optically active molecular arrays.
authors: Department of Chemical Engineering and Materials Science, University of California, Davis, Davis, California 95616, United States.
authors: Maria O
authors: MO
authors: Ogunyankin
authors: Dale L
authors: DL
authors: Huber
authors: Darryl Y
authors: DY
authors: Sasaki
authors: Marjorie L
authors: ML
authors: Longo
mesh_terms: D003300:Copper / Q000737:chemistry; D049452:Green Fluorescent Proteins / Q000737:chemistry*; D006639:Histidine / Q000737:chemistry; D008055:Lipids / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry*; D015394:Molecular Structure; D010316:Particle Size; D013499:Surface Properties
chemical_list: D008055:Lipids; D008565:Membrane Proteins; D049452:Green Fluorescent Proteins; D006639:Histidine; D003300:Copper