title: VPPIPP and IPPVPP: two hexapeptides innovated to exert antihypertensive activity.
abstract: In this study, two hexapeptides of IPPVPP and VPPIPP were innovated by using two commercial antihypertensive peptides IPP and VPP as two domains cis-linked and trans-linked, respectively. The IPPVPP and VPPIPP were chemically synthesized and evaluated for the antihypertensive activity in vitro/vivo. The in vitro ACE-inhibitory study showed that VPPIPP (34.71 ± 4.38%) has a significantly stronger activity than that of IPPVPP (13.17 ± 0.25%) at a treatment concentration of 10 µmol/L, but it was weaker than the commercial IPP (56.97 ± 2.40%) (P<0.05). However, VPPIPP, IPPVPP, and IPP lowered the systolic blood pressure by 21 ± 0.9%, 17.4 ± 1.3% and 17.5 ± 0.9%, respectively, in rats at 1.5 mg/kg body weight dosage. The result was consistent with the mRNA level of sarcoplasmic reticulum Ca(2+), Mg(2+) -ATPase Gene (SERCA 2a) in rat hearts. Additionally, VPPIPP and IPPVPP showed no negative impact on blood glycometabolism. The results suggested that the two hexapeptides could be potent bioactive peptides in functional foods for people with high blood pressure.
authors: Department of Food Science and Engineering, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
authors: Fengyun
authors: F
authors: Ding
authors: Bingjun
authors: B
authors: Qian
authors: Xin
authors: X
authors: Zhao
authors: Shanqi
authors: S
authors: Shen
authors: Yun
authors: Y
authors: Deng
authors: Danfeng
authors: D
authors: Wang
authors: Feng
authors: F
authors: Zhang
authors: Zhongquan
authors: Z
authors: Sui
authors: Pu
authors: P
authors: Jing
mesh_terms: D000595:Amino Acid Sequence; D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry* / Q000627:therapeutic use*; D000818:Animals; D000959:Antihypertensive Agents / Q000737:chemistry* / Q000627:therapeutic use*; D001786:Blood Glucose / Q000378:metabolism; D001794:Blood Pressure / Q000187:drug effects; D008297:Male; D010455:Peptides / Q000737:chemistry* / Q000627:therapeutic use*; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D011817:Rabbits; D051381:Rats; D011918:Rats, Inbred SHR; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000235:genetics; D015533:Transcriptional Activation / Q000187:drug effects
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D001786:Blood Glucose; D010455:Peptides; D007703:Peptidyl-Dipeptidase A; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases
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