title: Matrix metalloproteinase, vitamin A and methylprednisolone effects on experimentally induced amyloid arthropathy.
abstract: We evaluated the role of some matrix metalloproteinases (MMPs) in enhancing the effect of vitamin A and the inhibiting effect of methylprednisolone on amyloid arthropathy in brown layer chicks. We used 100 one-day-old Isa brown layer chicks. The chicks were allocated to one of four groups as follows: negative control group (I), vitamin A group (II), positive control group (III) and methylprednisolone group (IV). Amyloid arthropathy was induced by injections of complete Freund's adjuvant into the left intertarsal joints of the chicks. Serum vitamin A and tissue MMP (MMP-1, MMP-2, MMP-9) levels were measured and differences among the groups were investigated. Serum vitamin A rates (μg/dl) were: 63.57 ± 4.10, 47.13 ± 10.62, 53.26 ± 10.79, 98.48 ± 8.20 in groups I, II, III and IV, respectively (p < 0.001). MMP-1, MMP-2 and MMP-9 levels were evaluated in tissues from the chickens with amyloid arthropathy. Methylprednisolone significantly suppressed the release of MMP-1 and MMP-2, and increased the release of MMP-9 in birds with amyloid arthropathy. In addition, vitamin A significantly increased the release of MMP-1, MMP-2 and MMP-9.
authors: Department of Pathology, Faculty of Veterinary Medicine, Afyon Kocatepe Üniversity, 03200, Afyonkarahisar, Turkey. alpers@aku.edu.tr
authors: A
authors: A
authors: Sevimli
authors: A
authors: A
authors: Yağcı
authors: A
authors: A
authors: Bülbül
mesh_terms: D000682:Amyloid; D000686:Amyloidosis / Q000139:chemically induced*; D000818:Animals; D002645:Chickens; D005620:Freund's Adjuvant; D007150:Immunohistochemistry; D020782:Matrix Metalloproteinases / Q000097:blood / Q000378:metabolism*; D008775:Methylprednisolone / Q000494:pharmacology* / Q000627:therapeutic use; D013583:Synovial Membrane / Q000187:drug effects; D013640:Tarsal Joints / Q000187:drug effects*; D014801:Vitamin A / Q000097:blood / Q000494:pharmacology*
chemical_list: D000682:Amyloid; D014801:Vitamin A; D005620:Freund's Adjuvant; D020782:Matrix Metalloproteinases; D008775:Methylprednisolone