title: High vitamin D3 diet administered during active colitis negatively affects bone metabolism in an adoptive T cell transfer model.
abstract: Decreased bone mineral density (BMD) represents an extraintestinal complication of inflammatory bowel disease (IBD). Vitamin D₃ has been considered a viable adjunctive therapy in IBD. However, vitamin D₃ plays a pleiotropic role in bone modeling and regulates the bone formation-resorption balance, depending on the physiological environment, and supplementation during active IBD may have unintended consequences. We evaluated the effects of vitamin D₃ supplementation during the active phase of disease on colonic inflammation, BMD, and bone metabolism in an adoptive IL-10-/- CD4⁺ T cell transfer model of chronic colitis. High-dose vitamin D₃ supplementation for 12 days during established disease had negligible effects on mucosal inflammation. Plasma vitamin D₃ metabolites correlated with diet, but not disease, status. Colitis significantly reduced BMD. High-dose vitamin D₃ supplementation did not affect cortical bone but led to a further deterioration of trabecular bone morphology. In mice fed a high vitamin D₃ diet, colitis more severely impacted bone formation markers (osteocalcin and bone alkaline phosphatase) and increased bone resorption markers, ratio of receptor activator of NF-κB ligand to osteoprotegrin transcript, plasma osteoprotegrin level, and the osteoclast activation marker tartrate-resistant acid phosphatase (ACp5). Bone vitamin D receptor expression was increased in mice with chronic colitis, especially in the high vitamin D₃ group. Our data suggest that vitamin D₃, at a dose that does not improve inflammation, has no beneficial effects on bone metabolism and density during active colitis or may adversely affect BMD and bone turnover. These observations should be taken into consideration in the planning of further clinical studies with high-dose vitamin D₃ supplementation in patients with active IBD.
authors: Department of Pediatrics, Steele Children's Research Center, University of Arizona Health Sciences Center, Tucson, AZ 85724, USA.
authors: C B
authors: CB
authors: Larmonier
authors: R-M T
authors: RM
authors: McFadden
authors: F M
authors: FM
authors: Hill
authors: R
authors: R
authors: Schreiner
authors: R
authors: R
authors: Ramalingam
authors: D G
authors: DG
authors: Besselsen
authors: F K
authors: FK
authors: Ghishan
authors: P R
authors: PR
authors: Kiela
mesh_terms: D019264:Adoptive Transfer; D066258:Amphiregulin; D000821:Animal Feed; D000818:Animals; D015519:Bone Density / Q000187:drug effects; D001842:Bone and Bones / Q000187:drug effects* / Q000378:metabolism*; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology; D002762:Cholecalciferol / Q000008:administration & dosage / Q000494:pharmacology*; D002908:Chronic Disease; D003092:Colitis / Q000150:complications* / Q000378:metabolism; D004032:Diet; D066255:EGF Family of Proteins; D017353:Gene Deletion; D006023:Glycoproteins / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D014815:Vitamins / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D066258:Amphiregulin; C587849:Areg protein, mouse; D066255:EGF Family of Proteins; D006023:Glycoproteins; D036341:Intercellular Signaling Peptides and Proteins; D014815:Vitamins; D016753:Interleukin-10; D002762:Cholecalciferol
keywords: 1,25-dihydroxyvitamin D3; bone mineral density; bone turnover; inflammatory bowel disease
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