title: Brucella melitensis 16MΔhfq attenuation confers protection against wild-type challenge in BALB/c mice.
abstract: Brucellosis is a globally distributed zoonotic disease that causes animal and human diseases. Although effective, the current Brucella vaccines (Rev.1 and M5-90) have several drawbacks. The first involves residual virulence for animals and humans and the second is the inability to differentiate natural infection from that caused by vaccination. Therefore, Brucella melitensis 16M hfq mutant (16MΔhfq) was constructed to overcome these drawbacks. Similarly to Rev.1 and M5-90, 16MΔhfq reduces survival in macrophages and mice and induces strong protective immunity in BALB/c mice. Moreover, these vaccines elicit anti-Brucella-specific IgG1 and IgG2a subtype responses and induce secretion of gamma interferon and interleukin-4. The Hfq antigen also allows serological differentiation between infected and vaccinated animals. These results show that 16MΔhfq is an ideal live attenuated vaccine candidate against virulent Brucella melitensis 16M infection. It will be further evaluated in sheep.
authors: College of Animal Science and Technology, Shihezi University, North 4 Road, Shihezi 832003, China.
authors: Junbo
authors: J
authors: Zhang
authors: Fei
authors: F
authors: Guo
authors: Chuangfu
authors: C
authors: Chen
authors: Zhiqiang
authors: Z
authors: Li
authors: Hui
authors: H
authors: Zhang
authors: Yuanzhi
authors: Y
authors: Wang
authors: Ke
authors: K
authors: Zhang
authors: Guoqing
authors: G
authors: Du
authors: Yuefeng
authors: Y
authors: Li
authors: Jiangde
authors: J
authors: Wang
authors: Tong
authors: T
authors: Jian
authors: Zhen
authors: Z
authors: Wang
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D002004:Brucella Vaccine / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D017347:Brucella melitensis / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity*; D002006:Brucellosis / Q000276:immunology / Q000517:prevention & control*; D002470:Cell Survival; D004195:Disease Models, Animal; D005260:Female; D017353:Gene Deletion; D035001:Host Factor 1 Protein / Q000235:genetics*; D007074:Immunoglobulin G / Q000097:blood; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D008264:Macrophages / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D016019:Survival Analysis; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D000907:Antibodies, Bacterial; D002004:Brucella Vaccine; D035001:Host Factor 1 Protein; D007074:Immunoglobulin G; D014613:Vaccines, Attenuated; D037521:Virulence Factors; D015847:Interleukin-4; D007371:Interferon-gamma
keywords: Brucella melitensis; Hfq; live attenuated; vaccine candidate