title: Effect of chondroitinase ABC on adhesion and behavior of synovial membrane-derived mesenchymal stem cells in rabbit partial-thickness chondral defects.
abstract: Transplanted cells may have difficulty attaching to the surface of partial-thickness chondral lesions because of the anti-adhesive properties of the proteoglycan rich matrix. Therefore, the current study attempts to evaluate the effect of chondroitinase ABC (chABC) on the adhesion and behavior of transplanted synovial membrane-derived mesenchymal stem cells (SDSCs) in rabbit partial-thickness chondral defects. In ex vivo adhesion experiments, chABC treatment (0.1 U/ml) was increased in SDSC attachment to the cartilage explants, and significantly diminished by pretreatment with neutralizing antibody against fibronectin. In the in vivo experiments, 1 day and 4 weeks after the chABC treatment (0.1 and 1 U/ml), the immunoreactivity (IR) against CS-56 (intact chondroitin sulfate antibody) was markedly decreased; however, the IR of 2B6 (stub of the chondroitin 4-sulfate chain), 3B3 (stub of the chondroitin 6-sulfate chain), and fibronectin was increased. At 12 weeks, this IR returned to normal except in the high-dose chABC-treated group (1 U/ml). Furthermore, the attachment of SDSCs to the chondral defects after chABC treatment was increased at 7 days compared with that in the chondral defects pretreated with saline. However, the tissue repaired by SDSCs was negatively stained for type II collagen at 12 weeks. In conclusion, these results showed that the exposure to fibronectin by chABC treatment enhances the attachment of SDSCs to partial-thickness chondral defects. However, the tissue regenerated by SDSCs showed lack of hyaline cartilage regeneration. Thus, to understand the fate of transplanted MSCs in cartilage defect is very important for successful cell therapies.
authors: Department of Orthopedic Surgery, Seoul National University College of Medicine, #28 Yongondong, Chongnogu, Seoul, 110-744, Republic of Korea.
authors: Jae-Chul
authors: JC
authors: Lee
authors: Hyun Jin
authors: HJ
authors: Min
authors: Sahnghoon
authors: S
authors: Lee
authors: Sang Cheol
authors: SC
authors: Seong
authors: Myung Chul
authors: MC
authors: Lee
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000293:injuries* / Q000473:pathology; D002448:Cell Adhesion / Q000187:drug effects*; D017690:Cell Transplantation; D019765:Chondroitin ABC Lyase / Q000008:administration & dosage / Q000494:pharmacology*; D002809:Chondroitin Sulfates / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005353:Fibronectins / Q000378:metabolism; D050724:Fractures, Cartilage / Q000473:pathology; D007270:Injections, Intra-Articular; D039081:Integrin alpha5beta1 / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D011817:Rabbits; D013264:Stifle; D013583:Synovial Membrane / Q000187:drug effects* / Q000473:pathology
chemical_list: D005353:Fibronectins; D039081:Integrin alpha5beta1; D002809:Chondroitin Sulfates; D019765:Chondroitin ABC Lyase