title: Using high-throughput in situ plate screening to evaluate the effect of dehydration on protein crystals.
abstract: Crystal dehydration is a post-crystallization technique that can potentially improve the diffraction of macromolecular crystals. There are currently several ways of undertaking this process; however, dehydration experiments are often limited in their throughput and require prior manipulation of the samples. In the present study, a novel method is proposed that uses in situ plate screening to assess the effect of dehydration by combining the throughput of 96-well crystallization plates with direct X-ray feedback on crystal diffraction quality.
authors: Life Science, Diamond Light Source, Harwell Science and Innovation Campus, Didcot, Oxfordshire OX11 0DE, England.
authors: Alice
authors: A
authors: Douangamath
authors: Pierre
authors: P
authors: Aller
authors: Petra
authors: P
authors: Lukacik
authors: Juan
authors: J
authors: Sanchez-Weatherby
authors: Isabel
authors: I
authors: Moraes
authors: Jose
authors: J
authors: Brandao-Neto
mesh_terms: D003460:Crystallization / Q000295:instrumentation / Q000379:methods; D018360:Crystallography, X-Ray; D057166:High-Throughput Screening Assays / Q000379:methods*; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins
keywords: dehydration; high throughput; in situ plate screening