title: The adsorptive behavior of albumin and lysozyme proteins on rod-shaped and plate-shaped hydroxyapatite.
abstract: The adsorption behavior of albumin (BSA) and lysozyme (LSZ) on rod-shaped and plate-shaped hydroxyapatite (HA) was investigated to evaluate the influence of crystal orientation and morphology on the selective protein adsorption of HA. The rod-shaped HA was prepared by hydrothermal treatment from β-tricalcium phosphate (β-TCP) in H3PO4 solution (pH 2.0 and 4.0 for HA-pH 2.0 and HA-pH 4.0). The plate-shaped HA was synthesized by hydrolysis of CaHPO4-2H2O (DCPD) in NaOH solution at 40°C and 80°C (HA-40°C and HA-80°C). The synthesized HA was characterized using scanning electron microscopy (SEM) and X-ray diffractometry (XRD). HA-pH 2.0 and HA-pH 4.0 produced rod-shaped crystals that were highly oriented to the a-face plane, whereas HA-40°C and HA-80°C showed a plate-like shape and a c-face preferred orientation. The peak intensity ratio I(300)/I(002) (a/c intensity ratio) from the XRD patterns increased in the following order: HA-80°C, HA-40°C, HA-pH 2.0 and HA-pH 4.0. It also increased as the Ca/P ratio decreased. The amount of adsorbed BSA increased in the following order: HA-pH 4.0, HA-pH 2.0, HA-40°C and HA-80°C. The amount of adsorbed LSZ on HA increased in the following order--HA-pH 2.0, HA-pH 4.0, HA-40°C and HA-80°C--with a corresponding decrease in the a/c intensity ratio. The BSA/LSA adsorption ratio increased with the a/c intensity ratio in the range of 3.3-8.9, and the BSA and LSZ were selectively adsorbed on HA, depending on the crystal shape.
authors: Department of Mechanical Engineering, Ibaraki University, Hitachi, Ibaraki, Japan. ozeki@mx.ibaraki.ac.jp
authors: K
authors: K
authors: Ozeki
authors: T
authors: T
authors: Hoshino
authors: H
authors: H
authors: Aoki
authors: T
authors: T
authors: Masuzawa
mesh_terms: D000327:Adsorption; D000818:Animals; D018786:Bone Substitutes / Q000737:chemistry*; D002130:Calcium Phosphates / Q000737:chemistry; D002417:Cattle; D003460:Crystallization; D017886:Durapatite / Q000737:chemistry*; D009113:Muramidase / Q000302:isolation & purification*; D033362:Powder Diffraction; D012710:Serum Albumin, Bovine / Q000302:isolation & purification*; D014961:X-Ray Diffraction
chemical_list: D018786:Bone Substitutes; D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; D012710:Serum Albumin, Bovine; D017886:Durapatite; D009113:Muramidase