title: Identification of HLA class I-binding peptides derived from unique cancer-associated proteins by mass spectrometric analysis.
abstract: OBJECTIVE
  Since antigenic peptides of the cancer-associated antigens presented on human leukocyte antigen (HLA) molecules are recognized by specific cytotoxic T-lymphocytes, they have the potential to becoming effective peptide vaccines for cancer immunotherapy.
  
  
  METHODS
  Peptides binding to HLA-A*0201 and HLA-A*2402 obtained from human prostate cancer cells by acid-elution were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and source proteins of the peptides were determined based on the HLA-binding capacity listed on the Syfpeithi.
  
  
  RESULTS
  We identified TKLSA possibly derived from absent in melanoma 1-like protein (AIM1L), and RLRYT from trans-membrane protein-191C (TMEM 191C) or c20orf201. Messenger RNAs encoding these proteins were expressed in various cancer cell types but none or very few in non-cancerous tissues except for testis, cerebellum and ovary.
  
  
  CONCLUSIONS
  HLA class I-binding peptides of unique cancer-associated proteins were identified by MS analysis, and might become a promising tool for the generation of novel cancer vaccines.
authors: Department of Oncology, Institute of DNA Medicine, The Jikei University School of Medicine, 3-25-8 Nishi-shimbashi Minato-ku, Tokyo 105-8461 Japan. ykama@jikei.ac.jp
authors: Yuko
authors: Y
authors: Kamata
authors: Akiko
authors: A
authors: Kuhara
authors: Takeo
authors: T
authors: Iwamoto
authors: Kazumi
authors: K
authors: Hayashi
authors: Shigeo
authors: S
authors: Koido
authors: Takahiro
authors: T
authors: Kimura
authors: Shin
authors: S
authors: Egawa
authors: Sadamu
authors: S
authors: Homma
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D002853:Chromatography, Liquid; D015395:Histocompatibility Antigens Class I / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D009369:Neoplasms / Q000276:immunology / Q000378:metabolism*; D010446:Peptide Fragments / Q000276:immunology / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; D015395:Histocompatibility Antigens Class I; D010446:Peptide Fragments
keywords: HLA class-I; antigenic peptide; cancer associated antigen; mass spectrometric analysis; prostate cancer