title: Isolation of a wheat (Triticum aestivum L.) mutant in ABA 8'-hydroxylase gene: effect of reduced ABA catabolism on germination inhibition under field condition.
abstract: Pre-harvest sprouting, the germination of mature seeds on the mother plant under moist condition, is a serious problem in cereals. To investigate the effect of reduced abscisic acid (ABA) catabolism on germination in hexaploid wheat (Triticum aestivum L.), we cloned the wheat ABA 8'-hydroxyase gene which was highly expressed during seed development (TaABA8'OH1) and screened for mutations that lead to reduced ABA catabolism. In a screen for natural variation, one insertion mutation in exon 5 of TaABA8'OH1 on the D genome (TaABA8'OH1-D) was identified in Japanese cultivars including 'Tamaizumi'. However, a single mutation in TaABA8'OH1-D had no clear effect on germination inhibition in double haploid lines. In a screen for a mutation, one deletion mutant lacking the entire TaABA8'OH1 on the A genome (TaABA8'OH1-A), TM1833, was identified from gamma-ray irradiation lines of 'Tamaizumi'. TM1833 (a double mutant in TaABA8'OH1-A and TaABA8'OH1-D) showed lower TaABA8'OH1 expression, higher ABA content in embryos during seed development under field condition and lower germination than those in 'Tamaizumi' (a single mutant in TaABA8'OH1-D). These results indicate that reduced ABA catabolism through mutations in TaABA8'OH1 may be effective in germination inhibition in field-grown wheat.
authors: NARO Institute of Crop Science , 2-1-18 Kannondai, Tsukuba, Ibaraki 305-8518, Japan.
authors: Makiko
authors: M
authors: Chono
authors: Hitoshi
authors: H
authors: Matsunaka
authors: Masako
authors: M
authors: Seki
authors: Masaya
authors: M
authors: Fujita
authors: Chikako
authors: C
authors: Kiribuchi-Otobe
authors: Shunsuke
authors: S
authors: Oda
authors: Hisayo
authors: H
authors: Kojima
authors: Daisuke
authors: D
authors: Kobayashi
authors: Naoto
authors: N
authors: Kawakami
keywords: ABA 8′-hydroxylase; Triticum aestivum L.; abscisic acid; dormancy; germination; pre-harvest sprouting; wheat
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