title: [Screening of hepatocyte proteins interacting with hepatitis B virus X protein using CytoTrap yeast two-hybrid technique].
abstract: OBJECTIVE
  To screen the hepatocyte proteins that interact with hepatitis B virus X protein (HBx).
  
  
  METHODS
  The recombinant plasmid pSos-HBx was constructed by inserting Sos-HBx fragment into the bait vector, and after sequence verification the plasmid was transformed into competent yeast cells. The expression and self-activation of Sos-HBx protein was detected in the yeast cells. The hepatocyte proteins interacting with the bait protein was screened with CytoTrap yeast two-hybrid technique.
  
  
  RESULTS
  The reconstructed plasmid harboring HBx gene expressed Sos-HBx protein in the yeast cells without self-activation of the protein. CytoTrap yeast two-hybrid system identified 6 hepatocyte proteins that interacted with HBx, including fibronectin 1, translationally controlled tumor protein, IQ motif and WD repeats 1, follistatin, orosomucoid 1, and disulfide isomerase family A member 3.
  
  
  CONCLUSIONS
  Six HBx-binding hepatocyte proteins have been identified using the CytoTrap yeast two-hybrid system, which provides clues for further investigation of the role of HBx protein in hepatitis and liver cancer.
authors: Faculty of Life Science and Technology, Kunming University of Science and Technology, China. jiaobaihai@126.com
authors: Baihai
authors: B
authors: Jiao
authors: Yan
authors: Y
authors: Wen
authors: Xiaojia
authors: X
authors: Liu
authors: Yue
authors: Y
authors: Feng
authors: Amei
authors: A
authors: Zhang
authors: Li
authors: L
authors: Liu
authors: Xueshan
authors: X
authors: Xia
mesh_terms: D005822:Genetic Vectors; D022781:Hepatocytes / Q000378:metabolism*; D006801:Humans; D010957:Plasmids; D054730:Protein Interaction Domains and Motifs; D011506:Proteins / Q000378:metabolism; D015534:Trans-Activators / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D054334:Viral Regulatory and Accessory Proteins
chemical_list: D011506:Proteins; D015534:Trans-Activators; D054334:Viral Regulatory and Accessory Proteins; C057628:hepatitis B virus X protein