title: Structural changes in dengue virus when exposed to a temperature of 37°C.
abstract: Previous binding studies of antibodies that recognized a partially or fully hidden epitope suggest that insect cell-derived dengue virus undergoes structural changes at an elevated temperature. This was confirmed by our cryo-electron microscopy images of dengue virus incubated at 37°C, where viruses change their surface from smooth to rough. Here we present the cryo-electron microscopy structures of dengue virus at 37°C. Image analysis showed four classes of particles. The three-dimensional (3D) map of one of these classes, representing half of the imaged virus population, shows that the E protein shell has expanded and there is a hole at the 3-fold vertices. Fitting E protein structures into the map suggests that all of the interdimeric and some intradimeric E protein interactions are weakened. The accessibility of some previously found cryptic epitopes on this class of particles is discussed.
authors: Program in Emerging Infectious Diseases, Duke-NUS Graduate Medical School, Singapore.
authors: Guntur
authors: G
authors: Fibriansah
authors: Thiam-Seng
authors: TS
authors: Ng
authors: Victor A
authors: VA
authors: Kostyuchenko
authors: Jaime
authors: J
authors: Lee
authors: Sumarlin
authors: S
authors: Lee
authors: Jiaqi
authors: J
authors: Wang
authors: Shee-Mei
authors: SM
authors: Lok
mesh_terms: D000818:Animals; D002460:Cell Line; D020285:Cryoelectron Microscopy; D009033:Culicidae; D003716:Dengue Virus / Q000737:chemistry* / Q000648:ultrastructure*; D004591:Electrophoresis, Polyacrylamide Gel; D008958:Models, Molecular; D011487:Protein Conformation; D012394:Rosaniline Dyes; D013696:Temperature; D014759:Viral Envelope Proteins / Q000648:ultrastructure*
chemical_list: D012394:Rosaniline Dyes; D014759:Viral Envelope Proteins; C048139:Coomassie blue
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