title: Morphometric evaluation and nonclassical criteria for the diagnosis of HPV infection and cytological atypia in cervical samples.
abstract: Herein, we evaluated cervical samples from normal tissue or HPV-infected tissue, to determine if the relative nuclear/cytoplasmic ratio (NA/CA) and the presence of nonclassical cytological criteria are a novel cytological criterion for the diagnosis of HPV. Significantly, larger NA/CA ratios were found for the HPV-ATYPIA+ and HPV+ATYPIA+ groups compared with HPV-ATYPIA- group, regardless of collection method. For the samples collected with a spatula, only three samples from the HPV-ATIPIA- group showed four or more nonclassical parameters (i.e., were positive), while a larger number of the samples in the HPV-ATYPIA+, HPV+ATYPIA-, and HPV+ATYPIA+ groups were positive (13, 4, and 13 samples, respectively). Among those collected with a brush, no sample showed four or more nonclassical criteria in the HPV-ATYPIA- group, while a number of samples were positive in the HPV-ATYPIA+, HPV+ATYPIA-, and HPV+ATYPIA+ groups (4, 3, and 4 samples, respectively). HPV infection was associated with significant morphometrical changes; no increase in the NA/CA ratio was found in the HPV+ATYPIA- samples, compared with the HPV-ATIPIA- samples collected with either a spatula or a brush. In conclusion, by including nonclassical cytological criteria into the patient diagnosis, we were able to reduce the number of false negative and false positive HPV diagnoses made using conventional cytology alone.
authors: Departamento de Análises Clínicas, Escola de Farmácia, Universidade Federal de Ouro Preto (UFOP), Ouro Preto, MG, Brasil.
authors: Dandara Emery Morais
authors: DE
authors: Sana
authors: Priscila
authors: P
authors: Mayrink de Miranda
authors: Bruna Caroline Vieira
authors: BC
authors: Pitol
authors: Mariana Soares
authors: MS
authors: Moran
authors: Nayara Nascimento Toledo
authors: NN
authors: Silva
authors: Ismael Dali Cotrim
authors: ID
authors: Guerreiro da Silva
authors: Rita
authors: R
authors: de Cássia Stocco
authors: Willy
authors: W
authors: Beçak
authors: Angélica Alves
authors: AA
authors: Lima
authors: Cláudia Martins
authors: CM
authors: Carneiro
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002467:Cell Nucleus / Q000473:pathology; D002584:Cervix Uteri / Q000473:pathology* / Q000821:virology*; D003581:Cytodiagnosis / Q000379:methods*; D005260:Female; D006801:Humans; D008875:Middle Aged; D027383:Papillomaviridae / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology*; D014626:Vaginal Smears; D055815:Young Adult
keywords: HPV; cervix-vaginal cytology; molecular biology; morphometry; nonclassical criteria