title: Mitochondrial transcription factor A regulated ionizing radiation-induced mitochondrial biogenesis in human lung adenocarcinoma A549 cells.
abstract: Mitochondrial transcription factor A (TFAM), the first well-characterized transcription factor from vertebrate mitochondria, is closely related to mitochondrial DNA (mtDNA) maintenance and repair. Recent evidence has shown that the ratio of mtDNA to nuclearDNA (nDNA) is increased in both human cells and murine tissues after ionizing radiation (IR). However, the underlying mechanism has not as yet been clearly identified. In the present study, we demonstrated that in human lung adenocarcinoma A549 cells, expression of TFAM was upregulated, together with the increase of the relative mtDNA copy number and cytochrome c oxidase (COX) activity after α-particle irradiation. Furthermore, short hairpin RNA (shRNA)-mediated TFAM knockdown inhibited the enhancement of the relative mtDNA copy number and COX activity caused by α-particles. Taken together, our data suggested that TFAM plays a crucial role in regulating mtDNA amplification and mitochondrial biogenesis under IR conditions.
authors: School of Nuclear Science and Technology, University of Science and Technology of China, Hefei 230027, PR China.
authors: Jing
authors: J
authors: Yu
authors: Qisen
authors: Q
authors: Wang
authors: Ni
authors: N
authors: Chen
authors: Yuxiang
authors: Y
authors: Sun
authors: Xiaofei
authors: X
authors: Wang
authors: Lijun
authors: L
authors: Wu
authors: Shaopeng
authors: S
authors: Chen
authors: Hang
authors: H
authors: Yuan
authors: An
authors: A
authors: Xu
authors: Jun
authors: J
authors: Wang
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology* / Q000503:physiopathology*; D045744:Cell Line, Tumor; D056915:DNA Copy Number Variations / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004307:Dose-Response Relationship, Radiation; D003576:Electron Transport Complex IV / Q000378:metabolism*; D006801:Humans; D024101:Mitochondrial Proteins / Q000378:metabolism*; D063269:Mitochondrial Turnover / Q000528:radiation effects*; D011829:Radiation Dosage; D014157:Transcription Factors / Q000378:metabolism*; D015854:Up-Regulation / Q000528:radiation effects
chemical_list: D004272:DNA, Mitochondrial; D004268:DNA-Binding Proteins; D024101:Mitochondrial Proteins; C499631:TFAM protein, human; D014157:Transcription Factors; D003576:Electron Transport Complex IV
keywords: ionizing radiation; mitochondrial DNA; mitochondrial biogenesis; mitochondrial transcription factor A
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