title: Reduction of motor disorder in 6-OHDA-induced severe parkinsonism rats by post treatment with granulocyte-colony stimulating factor.
abstract: Granulocyte-colony stimulating factor (G-CSF) induced regeneration of dopaminergic neurons and improved behavior deficit in moderate Parkinson's disease (PD) model mice. Post treatment of G-CSF in severe PD model has not been addressed. A very severe PD model in rats was induced by a high dose 6-hydroxydopamine (6-OHDA) injected into the right medial forebrain bundle to evaluate therapeutic effects of G-CSF. G-CSF (50 microg/kg/day for five days) was given on the 9th day after the 6-OHDA injection. Rotational behavior was examined on the 9th and 28th days. Rats were killed on the 28th day and survival dopaminergic neurons in the substantia nigra, dopaminergic axons and dopaminergic receptor 2 in the striatum were examined. We, for the first time, demonstrated that post treatment with G-CSF reduced abnormal rotational behavior and increased the lesion to non-lesion ratio of dopaminergic fibers in the striatum, but the treatment promoted neither the increase in survival dopaminergic neurons nor the increase in dopaminergic receptor 2 expression. We conclude that post treatment with G-CSF can reduce the abnormal rotational behavior of severe PD rats primarily through relative increases in dopaminergic fibers of the lesion side in the striatum. Results of our study suggest therapeutic potentials of G-CSF for treating severe PD patients.
authors: Institute of Medical Sciences, Tzu Chi University, Hualien 97002, Taiwan, Republic of China.
authors: Chia-Hsin
authors: CH
authors: Liao
authors: Department of Neurosurgery, Buddhist Tzu Chi General Hospital, Hualien 97004, Taiwan, Republic of China.
authors: Shin-Yuan
authors: SY
authors: Chen
authors: Institute of Medical Sciences, Tzu Chi University, Hualien 97002, Taiwan, Republic of China.
authors: Jon-Son
authors: JS
authors: Kuo
authors: Institute of Medical Sciences, Tzu Chi University, Hualien 97002, Taiwan, Republic of China.
authors: Cheng-Yoong
authors: CY
authors: Pang
mesh_terms: D000818:Animals; D003342:Corpus Striatum / Q000378:metabolism; D004195:Disease Models, Animal; D059290:Dopaminergic Neurons / Q000187:drug effects*; D004353:Drug Evaluation, Preclinical; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D009416:Nerve Regeneration / Q000187:drug effects*; D016627:Oxidopamine / Q000008:administration & dosage; D020734:Parkinsonian Disorders / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017448:Receptors, Dopamine D2 / Q000378:metabolism; D013565:Sympatholytics / Q000008:administration & dosage
chemical_list: D017448:Receptors, Dopamine D2; D013565:Sympatholytics; D016179:Granulocyte Colony-Stimulating Factor; D016627:Oxidopamine