title: Identification of clinical biomarkers for pre-analytical quality control of blood samples.
abstract: BACKGROUND
  Pre-analytical conditions are key factors in maintaining the high quality of biospecimens. They are necessary for accurate reproducibility of experiments in the field of biomarker discovery as well as achieving optimal specificity of laboratory tests for clinical diagnosis. In research at the National Biobank of Korea, we evaluated the impact of pre-analytical conditions on the stability of biobanked blood samples by measuring biochemical analytes commonly used in clinical laboratory tests.
  
  
  METHODS
  We measured 10 routine laboratory analytes in serum and plasma samples from healthy donors (n = 50) with a chemistry autoanalyzer (Hitachi 7600-110). The analyte measurements were made at different time courses based on delay of blood fractionation, freezing delay of fractionated serum and plasma samples, and at different cycles (0, 1, 3, 6, 9) of freeze-thawing. Statistically significant changes from the reference sample mean were determined using the repeated-measures ANOVA and the significant change limit (SCL).
  
  
  RESULTS
  The serum levels of GGT and LDH were changed significantly depending on both the time interval between blood collection and fractionation and the time interval between fractionation and freezing of serum and plasma samples. The glucose level was most sensitive only to the elapsed time between blood collection and centrifugation for blood fractionation. Based on these findings, a simple formula (glucose decrease by 1.387 mg/dL per hour) was derived to estimate the length of time delay after blood collection. In addition, AST, BUN, GGT, and LDH showed sensitive responses to repeated freeze-thaw cycles of serum and plasma samples.
  
  
  CONCLUSIONS
  These results suggest that GGT and LDH measurements can be used as quality control markers for certain pre-analytical conditions (eg, delayed processing or repeated freeze-thawing) of blood samples which are either directly used in the laboratory tests or stored for future research in the biobank.
authors: Hyun Ju
authors: HJ
authors: Kang
authors: Soon Young
authors: SY
authors: Jeon
authors: Jae-Sun
authors: JS
authors: Park
authors: Ji Young
authors: JY
authors: Yun
authors: Han Na
authors: HN
authors: Kil
authors: Won Kyung
authors: WK
authors: Hong
authors: Mee-Hee
authors: MH
authors: Lee
authors: Jun-Woo
authors: JW
authors: Kim
authors: Jae-Pil
authors: JP
authors: Jeon
authors: Bok Ghee
authors: BG
authors: Han
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood*; D001800:Blood Specimen Collection / Q000379:methods* / Q000592:standards*; D015925:Cryopreservation; D005260:Female; D005615:Freezing; D006801:Humans; D008297:Male; D008875:Middle Aged; D011786:Quality Control; D012044:Regression Analysis; D044967:Serum / Q000378:metabolism
chemical_list: D015415:Biomarkers
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