title: MicroFilament Analyzer identifies actin network organizations in epidermal cells of Arabidopsis thaliana roots.
abstract: The plant cytoskeleton plays a crucial role in the cells' growth and development during different developmental stages and it undergoes many rearrangements. In order to describe the arrangements of the F-actin cytoskeleton in root epidermal cells of Arabidopsis thaliana, the recently developed software MicroFilament Analyzer (MFA) was exploited. This software enables high-throughput identification and quantification of the orientation of filamentous structures on digital images in a highly standardized and fast way. Using confocal microscopy and transgenic GFP-FABD2-GFP plants the actin cytoskeleton was visualized in the root epidermis. MFA analysis revealed that during the early stages of cell development F-actin is organized in a mainly random pattern. As the cells grow, they preferentially adopt a longitudinal organization, a pattern that is also preserved in the largest cells. In the evolution from young to old cells, an approximately even distribution of transverse, oblique or combined orientations is always present besides the switch from random to a longitudinal oriented actin cytoskeleton.
authors: University of Antwerp; Biology Department; Plant Growth and Development; Antwerpen, Belgium.
authors: Eveline
authors: E
authors: Jacques
authors: Michal
authors: M
authors: Lewandowski
authors: Jan
authors: J
authors: Buytaert
authors: Yves
authors: Y
authors: Fierens
authors: Jean-Pierre
authors: JP
authors: Verbelen
authors: Kris
authors: K
authors: Vissenberg
mesh_terms: D008841:Actin Cytoskeleton; D017360:Arabidopsis / Q000033:anatomy & histology* / Q000254:growth & development; D063245:Plant Development; D019441:Plant Epidermis / Q000033:anatomy & histology*; D018517:Plant Roots / Q000033:anatomy & histology / Q000254:growth & development; D012984:Software
keywords: Arabidopsis; actin cytoskeleton; development; microfilament analyzer; root epidermis
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