title: Description of Pseudomonas jessenii subsp. pseudoputida subsp. nov., amended description of Pseudomonas jessenii and description of Pseudomonas jessenii subsp. jessenii subsp. nov.
abstract: Fluorescent Pseudomonas putida CCM 3656 (ATCC 11250) was analysed according to the methods of polyphasic approach which were based on sequence analyses involving the rpoB and rrs genes, manual ribotyping using endonuclease HindIII, DNA base composition determination and DNA-DNA hybridization. The results obtained by these genotyping methods showed that the strain CCM 3656 is distant from P. putida taxon, which was supported with phenotype characterization represented by whole-cell protein profile analysis, matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry profiling and extended biotyping. The DNA-DNA hybridization experiments performed between the strain CCM 3656 and the closest relatives revealed 77 % similarity with Pseudomonas jessenii. However, the outcomes of sequencing, ribotyping and phenotype characterization allow distinguishing the studied strain from P. jessenii. On the basis of the obtained taxonomic data, we suggest reclassifying strain CCM 3656 to a novel subspecies of P. jessenii and propose naming P. jessenii subsp. pseudoputida subsp. nov. with CCM 3656(T) as type strain. Furthermore, we present an amended description of P. jessenii and proposal of P. jessenii subsp. jessenii subsp. nov.
authors: Czech Collection of Microorganisms Department of Experimental Biology, Faculty of Science, Masaryk University, Tvrdého 14, 602 00, Brno, Czech Republic, marekos@seznam.cz.
authors: Marcel
authors: M
authors: Kosina
authors: Ivana
authors: I
authors: Mašlaňová
authors: Andrea
authors: A
authors: Pascutti-Vávrová
authors: Ondrej
authors: O
authors: Sedo
authors: Matej
authors: M
authors: Lexa
authors: Pavel
authors: P
authors: Svec
authors: Ivo
authors: I
authors: Sedláček
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D020543:Proteome / Q000032:analysis; D011549:Pseudomonas / Q000737:chemistry / Q000145:classification* / Q000235:genetics; D021521:Ribotyping; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D004275:DNA, Ribosomal; D020543:Proteome; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit
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