title: Partial molar pregnancy after intracytoplasmic sperm injection occurring as a result of diploid sperm usage.
abstract: OBJECTIVE
  Partial molar pregnancies are rare conceptions characterized by having 69 rather than 46 chromosomes, the additional chromosome complement usually occurring as a result of fertilization of the ovum by two sperm. Although assisted conception with intracytoplasmic sperm injection (ICSI) should prevent the development of a partial molar pregnancy, occasional cases have been described after assisted conception using ICSI. The objective of this study was to investigate the cause of partial molar pregnancy in a couple who had undertaken assisted conception with ICSI.
  
  
  METHODS
  Fluorescent microsatellite genotyping of DNA from the couple and tissue from their partial molar pregnancy was performed in order to confirm diagnosis and investigate the origin of the additional chromosome set.
  
  
  RESULTS
  Genotyping confirmed that the partial molar tissue was triploid with an additional chromosome complement from the father. Genotyping of additional loci proximal to the centromere demonstrated that the two paternal sets of chromosomes originated in a single sperm with a double complement of paternal DNA resulting from non-reduction at the second meiotic division.
  
  
  CONCLUSIONS
  This study confirms that partial molar pregnancy may occur after assisted conception with ICSI and that this occurs as a result of fertilization with a diploid sperm.
authors: Department of Medical Oncology, Imperial College Healthcare NHS Trust, Charing Cross Hospital, London, UK. philip.savage@imperial.nhs.uk
authors: Philip
authors: P
authors: Savage
authors: Neil
authors: N
authors: Sebire
authors: Tom
authors: T
authors: Dalton
authors: Anna
authors: A
authors: Carby
authors: Michael J
authors: MJ
authors: Seckl
authors: Rosemary A
authors: RA
authors: Fisher
mesh_terms: D000328:Adult; D004171:Diploidy; D005260:Female; D005307:Fertilization in Vitro; D060005:Genotyping Techniques; D006801:Humans; D006828:Hydatidiform Mole / Q000235:genetics* / Q000473:pathology; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics; D011247:Pregnancy; D020554:Sperm Injections, Intracytoplasmic; D013094:Spermatozoa / Q000473:pathology*
references: Hum Reprod. 2000 Mar;15(3):594-8
references: 10686202
references: Hum Reprod. 2002 Jan;17(1):5-7
references: 11756352
references: Fertil Steril. 2008 Jul;90(1):49-55
references: 17953968
references: J Reprod Med. 2010 Jul-Aug;55(7-8):341-5
references: 20795349
references: Ann Hum Genet. 1982 Jul;46(3):223-31
references: 7125594
references: Lancet. 1978 Aug 12;2(8085):366
references: 79723
references: Lancet. 1992 Jul 4;340(8810):17-8
references: 1351601
references: Cancer Genet Cytogenet. 1982 Apr;5(4):309-20
references: 6284349
references: Am J Hum Genet. 2000 Jun;66(6):1807-20
references: 10801385
references: Fertil Steril. 2005 Feb;83(2):462-3
references: 15705393
references: Hum Reprod. 1997 Apr;12(4):805-8
references: 9159446
references: Reprod Biomed Online. 2004 Oct;9(4):442-6
references: 15511346