title: Role of nitric oxide in the pathogenesis of alloxan diabetes.
abstract: We studied the effects of N(w)-nitro-L-arginine (L-NNA), a nonselective inhibitor of NO synthases, on the severity of type 1 diabetes mellitus induced by subcutaneous injection of 130 mg/kg alloxan in August rats with high activity of NO system and in Wistar rats. Five days after alloxan injection, hyperglycemia levels after overnight fasting in August and Wistar rats were 27.1±3.7 and 22.0±1.1 mmol/liter, respectively (p<0.03). The mortality over 15 days after alloxan injection in August rats was higher than in Wistar rats (36 and 26%, respectively). L-NNA normalized glucose levels in diabetics of both groups. It completely prevented mortality in August and reduced it to 13% in Wistar rats. Body weight loss and polydipsia after L-NNA injection were also less pronounced in August rats. Plasma nitrite/nitrate concentrations in August rats were 32% higher than in Wistar rats, both in intact and diabetic rats. These data attest to an important role of NO in the pathogenesis of alloxan diabetes.
authors: Institute of Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow, Russia. belkinal@yandex.ru
authors: L M
authors: LM
authors: Belkina
authors: E A
authors: EA
authors: Smirnova
authors: O L
authors: OL
authors: Terekhina
authors: S V
authors: SV
authors: Kruglov
authors: E S
authors: ES
authors: Boichuk
mesh_terms: D000496:Alloxan; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000517:prevention & control*; D004791:Enzyme Inhibitors / Q000627:therapeutic use; D008297:Male; D009566:Nitrates / Q000097:blood; D009569:Nitric Oxide / Q000096:biosynthesis / Q000502:physiology*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors*; D009573:Nitrites / Q000097:blood; D019335:Nitroarginine / Q000627:therapeutic use*; D051381:Rats; D011919:Rats, Inbred Strains; D017208:Rats, Wistar
chemical_list: D001786:Blood Glucose; D004791:Enzyme Inhibitors; D009566:Nitrates; D009573:Nitrites; D019335:Nitroarginine; D009569:Nitric Oxide; D000496:Alloxan; D019001:Nitric Oxide Synthase