title: Bio-oil based biorefinery strategy for the production of succinic acid.
abstract: BACKGROUND
  Succinic acid is one of the key platform chemicals which can be produced via biotechnology process instead of petrochemical process. Biomass derived bio-oil have been investigated intensively as an alternative of diesel and gasoline fuels. Bio-oil could be fractionized into organic phase and aqueous phase parts. The organic phase bio-oil can be easily upgraded to transport fuel. The aqueous phase bio-oil (AP-bio-oil) is of low value. There is no report for its usage or upgrading via biological methods. In this paper, the use of AP-bio-oil for the production of succinic acid was investigated.
  
  
  RESULTS
  The transgenic E. coli strain could grow in modified M9 medium containing 20 v/v% AP-bio-oil with an increase in OD from 0.25 to 1.09. And 0.38 g/L succinic acid was produced. With the presence of 4 g/L glucose in the medium, succinic acid concentration increased from 1.4 to 2.4 g/L by addition of 20 v/v% AP-bio-oil. When enzymatic hydrolysate of corn stover was used as carbon source, 10.3 g/L succinic acid was produced. The obtained succinic acid concentration increased to 11.5 g/L when 12.5 v/v% AP-bio-oil was added. However, it decreased to 8 g/L when 50 v/v% AP-bio-oil was added. GC-MS analysis revealed that some low molecular carbon compounds in the AP-bio-oil were utilized by E. coli.
  
  
  CONCLUSIONS
  The results indicate that AP-bio-oil can be used by E. coli for cell growth and succinic acid production.
authors: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P. O. Box 353, No. 1 Zhongguancun North Second Street, Beijing 100190, P.R. China ; 2University of Chinese Academy of Sciences, Beijing 100049, R.P. China.
authors: Caixia
authors: C
authors: Wang
authors: Department of Chemical and Biochemical Engineering, Technical University of Denmark, Lyngby, DK-2800, Denmark ; Sino-Danish Center for Education and Research, Niels Jensensvej 2, DK-8000, Aarhus C, Denmark.
authors: Anders
authors: A
authors: Thygesen
authors: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P. O. Box 353, No. 1 Zhongguancun North Second Street, Beijing 100190, P.R. China ; 2University of Chinese Academy of Sciences, Beijing 100049, R.P. China.
authors: Yilan
authors: Y
authors: Liu
authors: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P. O. Box 353, No. 1 Zhongguancun North Second Street, Beijing 100190, P.R. China.
authors: Qiang
authors: Q
authors: Li
authors: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P. O. Box 353, No. 1 Zhongguancun North Second Street, Beijing 100190, P.R. China.
authors: Maohua
authors: M
authors: Yang
authors: 2University of Chinese Academy of Sciences, Beijing 100049, R.P. China ; State Key Laboratory of Multiple Complex Systems, Institute of Process Engineering, Chinese Academy of Sciences, No. 1 Zhongguancun North Second Street, P. O. Box 353, , Beijing 100190, P.R. China.
authors: Dan
authors: D
authors: Dang
authors: State Key Laboratory of Multiple Complex Systems, Institute of Process Engineering, Chinese Academy of Sciences, No. 1 Zhongguancun North Second Street, P. O. Box 353, , Beijing 100190, P.R. China.
authors: Ze
authors: Z
authors: Wang
authors: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P. O. Box 353, No. 1 Zhongguancun North Second Street, Beijing 100190, P.R. China.
authors: Yinhua
authors: Y
authors: Wan
authors: State Key Laboratory of Multiple Complex Systems, Institute of Process Engineering, Chinese Academy of Sciences, No. 1 Zhongguancun North Second Street, P. O. Box 353, , Beijing 100190, P.R. China.
authors: Weigang
authors: W
authors: Lin
authors: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P. O. Box 353, No. 1 Zhongguancun North Second Street, Beijing 100190, P.R. China.
authors: Jianmin
authors: J
authors: Xing
references: Metab Eng. 2011 Jan;13(1):76-81
references: 21040799
references: Bioresour Technol. 2010 May;101(9):3292-4
references: 20061143
references: Bioresour Technol. 2012 Jul;116:366-71
references: 22534370
references: Appl Microbiol Biotechnol. 2002 Apr;58(5):663-8
references: 11956751
references: J Chromatogr A. 2012 Sep 28;1257:131-40
references: 22897863
references: Curr Opin Microbiol. 2006 Jun;9(3):268-74
references: 16617034
references: Bioresour Technol. 2011 Jan;102(2):1704-8
references: 20801644
references: Bioresour Technol. 2011 Mar;102(6):4304-12
references: 21277771
references: Appl Microbiol Biotechnol. 2007 Sep;76(4):727-40
references: 17609945