title: [Immunostimulator CH2a bearing thiazolidin-4-one promotes the function of human iNKT cells in vitro].
abstract: OBJECTIVE
  To explore the immunostimulatory effect of immunostimulant CH2a bearing thiazolidin-4-one on human invariant natural killer T (iNKT) cell function in vitro.
  
  
  METHODS
  Peripheral blood mononuclear cells (PBMCs) were obtained from healthy adults, and then amplified with α-Galcer and IL-2 in vitro. The iNKT cells were isolated from these proliferating cells by magnetic-activated cell sorting (MACS) method. The purified iNKT cells were labled with 5-(and 6)-carboxyfluorescein diacetate succinimidyl ester (CFDA-SE) and then incubated with CH2a for functional analysis, including cell proliferation, expressions of IFN-γ and IL-4 by flow cytometry, proliferation rate and cytotoxicity by MTT assay. In additon, ELISA was performed to determine the levels of IFN-γ and IL-4 in cell culture media.
  
  
  RESULTS
  CH2a significantly promoted the proliferation of iNKT cells induced by IL-2 in vitro, stimulated the release of both IFN-r and IL-4, and led to the increase in IFN-γ/IL-4 ratio. More importantly, the cytotoxicity of iNKT was also markedly elevated under the stimulation of CH2a.
  
  
  CONCLUSIONS
  Immunostimulant CH2a probably promote the production of Th1-like cytokines and the differentiation of Th0 to Th1, so as to improve cellular immune function. In addition, CH2a could significantly enhance the cytotoxicity of iNKT cells.
authors: School of Medicine, College of Chemistry and Environmental Science, Hebei University, Baoding 071000, China. mengming127@163.com
authors: Ming
authors: M
authors: Meng
authors: Chunxiao
authors: C
authors: Li
authors: Zerui
authors: Z
authors: Wang
authors: Han
authors: H
authors: Hua
authors: Jianhong
authors: J
authors: Shi
authors: Hua
authors: H
authors: Chen
authors: Xiaoliu
authors: X
authors: Li
authors: Dongzhi
authors: D
authors: Chen
mesh_terms: D000328:Adult; D048708:Cell Growth Processes / Q000187:drug effects / Q000276:immunology; D005260:Female; D005699:Galactosylceramides / Q000276:immunology; D006801:Humans; D007114:Immunization / Q000379:methods; D007155:Immunologic Factors / Q000276:immunology / Q000494:pharmacology*; D007371:Interferon-gamma / Q000276:immunology; D007376:Interleukin-2 / Q000276:immunology; D015847:Interleukin-4 / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology; D008297:Male; D055611:Natural Killer T-Cells / Q000187:drug effects* / Q000276:immunology*; D013844:Thiazoles / Q000276:immunology / Q000494:pharmacology*
chemical_list: D005699:Galactosylceramides; D007155:Immunologic Factors; D007376:Interleukin-2; D013844:Thiazoles; C493154:alpha-galactosylceramide; D015847:Interleukin-4; D007371:Interferon-gamma