title: O-GlcNAc modification of the coat protein of the potyvirus Plum pox virus enhances viral infection.
abstract: O-GlcNAcylation is a dynamic protein modification which has been studied mainly in metazoans. We reported previously that an Arabidopsis thaliana O-GlcNAc transferase modifies at least two threonine residues of the Plum pox virus (PPV) capsid protein (CP). Now, six additional residues were shown to be involved in O-GlcNAc modification of PPV CP. CP O-GlcNAcylation was abolished in the PPV CP7-T/A mutant, in which seven threonines were mutated. PPV CP7-T/A infected Nicotiana clevelandii, Nicotiana benthamiana, and Prunus persica without noticeable defects. However, defects in infection of A. thaliana were readily apparent. In mixed infections of wild-type arabidopsis, the CP7-T/A mutant was outcompeted by wild-type virus. These results indicate that CP O-GlcNAcylation has a major role in the infection process. O-GlcNAc modification may have a role in virion assembly and/or stability as the CP of PPV CP7-T/A was more sensitive to protease digestion than that of the wild-type virus.
authors: Department of Plant Molecular Genetics, Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, Darwin 3, Madrid, Spain.
authors: José de Jesús
authors: Jde J
authors: Pérez
authors: Namrata D
authors: ND
authors: Udeshi
authors: Jeffrey
authors: J
authors: Shabanowitz
authors: Sergio
authors: S
authors: Ciordia
authors: Silvia
authors: S
authors: Juárez
authors: Cheryl L
authors: CL
authors: Scott
authors: Neil E
authors: NE
authors: Olszewski
authors: Donald F
authors: DF
authors: Hunt
authors: Juan Antonio
authors: JA
authors: García
mesh_terms: D000117:Acetylglucosamine / Q000378:metabolism*; D017360:Arabidopsis / Q000821:virology; D036022:Capsid Proteins / Q000737:chemistry / Q000378:metabolism*; D004252:DNA Mutational Analysis; D010935:Plant Diseases / Q000821:virology; D017801:Plum Pox Virus / Q000737:chemistry / Q000472:pathogenicity*; D011499:Protein Processing, Post-Translational; D027861:Prunus / Q000821:virology; D014026:Nicotiana / Q000821:virology
chemical_list: D036022:Capsid Proteins; C095320:coat protein, Plum pox virus; D000117:Acetylglucosamine
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