title: Electron microscopy and composition of raw acorn starch in relation to in vivo starch digestibility.
abstract: The structure and composition of starch play an important role as co-factors affecting raw starch digestibility: such features were investigated in raw acorn starch from the most diffused oak trees in the Mediterranean basin. A total of 620 whole ripe acorns from Holm (Quercus ilex L., n = 198), Downy (Quercus pubescens Willd., n = 207) and Cork (Quercus suber L., n = 215) oaks sampled on the Sardinia Isle (40° 56' 0'' N; 9° 4' 0'' E; 545 m above the mean sea level) in the same geographical area, were analyzed for their chemical composition. The starch contents ranged between 51.2% and 53.5% of dry matter. The starch granules displayed a spheroid/ovoid and cylindrical shape; on scanning electron microscopic (SEM) analyses, a bimodal distribution of starch granule size was observed both for Holm and Cork oak acorns, whereas the starch granules of Downy oak acorns showed diameters between 10.2 and 13.8 μm. The specific amylose to amylopectin ratio of acorn starch was 25.8%, 19.5% and 34.0% in the Holm, Downy and Cork oaks, respectively. The (13)C Nuclear Magnetic Resonance (NMR) signal analysis displayed a pivotal spectrum for the identification of the amylose peaks in raw acorn starch, as a basis for the amylose to amylopectin ratio determination.
authors: Research Unit of Animal Husbrandry Sciences, Dipartimento di Agraria, Università degli Studi di Sassari, via De Nicola, 07100 - Sassari, Italy. mgcappai@uniss.it
authors: Maria Grazia
authors: MG
authors: Cappai
authors: Giuseppe Andrea
authors: GA
authors: Alesso
authors: Giuseppa
authors: G
authors: Nieddu
authors: Marina
authors: M
authors: Sanna
authors: Walter
authors: W
authors: Pinna
mesh_terms: D004063:Digestion; D006801:Humans; D007558:Italy; D008855:Microscopy, Electron, Scanning; D008954:Models, Biological; D029963:Quercus / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D012639:Seeds / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D013213:Starch / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure*
chemical_list: D013213:Starch