title: Evaluation of the histologic changes in the fat-grafted facial skin: clinical trial.
abstract: BACKGROUND
  Fat grafting is increasingly common in plastic surgery procedures. The discovery of stem cells in fat tissue has given a new direction to the use of fat as a therapeutic tool for other patient conditions. Only one experimental study in rats shows dermal changes after application of lipofilling. For this reason, the authors conducted this study to evaluate skin changes in patients after application of the technique. This study aimed to observe histologic changes in the skin of patients undergoing fat grafting.
  
  
  METHODS
  Fat grafting was performed in the preauricular region on one side of patients undergoing face-lifts at the Jalisco Reconstructive Surgery Institute, Guadalajara, Mexico. Preauricular skin was used in this procedure as a withdrawal study and control condition. Hematoxylin-eosin and Masson staining was performed to assess dermal and epidermal thickness, vascularity, and collagen behavior. No inferential statistics were registered with the Wilcoxon test.
  
  
  RESULTS
  The study investigated 16 patients to observe statistically significant differences in dermal thickness, the presence of immature collagen (neoformation), and arteries. No difference in epidermal thickness was observed.
  
  
  CONCLUSIONS
  The study showed a regenerative effect with fat grafting that included an increased thickness of the dermis, collagen neoformation, and the presence of increased vascularity in local skin subjected to treatment.
  
  
  METHODS
  This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
authors: Jalisco Institute of Reconstructive Surgery, Guadalajara, Jalisco, Mexico. drpcovarrubias@gmail.com
authors: Patricio
authors: P
authors: Covarrubias
authors: Lázaro
authors: L
authors: Cárdenas-Camarena
authors: José
authors: J
authors: Guerrerosantos
authors: Lorena
authors: L
authors: Valenzuela
authors: Ismael
authors: I
authors: Espejo
authors: José Antonio
authors: JA
authors: Robles
authors: Sebastián
authors: S
authors: Gioia
mesh_terms: D000273:Adipose Tissue / Q000637:transplantation*; D000328:Adult; D000368:Aged; D003094:Collagen / Q000378:metabolism*; D005260:Female; D006801:Humans; D015187:Lipectomy; D008297:Male; D008875:Middle Aged; D019651:Plastic Surgery Procedures; D012867:Skin / Q000098:blood supply / Q000473:pathology*; D014182:Transplantation, Autologous
chemical_list: D003094:Collagen