title: Compartmentalization of GABAergic inhibition by dendritic spines.
abstract: γ-aminobutyric acid-mediated (GABAergic) inhibition plays a critical role in shaping neuronal activity in the neocortex. Numerous experimental investigations have examined perisomatic inhibitory synapses, which control action potential output from pyramidal neurons. However, most inhibitory synapses in the neocortex are formed onto pyramidal cell dendrites, where theoretical studies suggest they may focally regulate cellular activity. The precision of GABAergic control over dendritic electrical and biochemical signaling is unknown. By using cell type-specific optical stimulation in combination with two-photon calcium (Ca(2+)) imaging, we show that somatostatin-expressing interneurons exert compartmentalized control over postsynaptic Ca(2+) signals within individual dendritic spines. This highly focal inhibitory action is mediated by a subset of GABAergic synapses that directly target spine heads. GABAergic inhibition thus participates in localized control of dendritic electrical and biochemical signaling.
authors: Department of Neurobiology, Yale School of Medicine, New Haven, CT 06510, USA.
authors: Chiayu Q
authors: CQ
authors: Chiu
authors: Gyorgy
authors: G
authors: Lur
authors: Thomas M
authors: TM
authors: Morse
authors: Nicholas T
authors: NT
authors: Carnevale
authors: Graham C R
authors: GC
authors: Ellis-Davies
authors: Michael J
authors: MJ
authors: Higley
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D000075402:Channelrhodopsins; D003198:Computer Simulation; D049229:Dendritic Spines / Q000502:physiology*; D005260:Female; D018698:Glutamic Acid / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008959:Models, Neurological; D019579:Neocortex / Q000502:physiology*; D009433:Neural Inhibition; D010775:Photic Stimulation; D017966:Pyramidal Cells / Q000502:physiology*; D013569:Synapses / Q000502:physiology; D005680:gamma-Aminobutyric Acid / Q000502:physiology*
chemical_list: D000075402:Channelrhodopsins; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D002118:Calcium
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