title: Low C-peptide levels and decreased expression of TNF and CD45 in children with high risk of type 1 diabetes.
abstract: Type 1 diabetes (T1D) patients have numeral and functional defects in peripheral immune cells, but the pre-diabetic period is fairly uncharacterized. Our aim was to analyze expression of immunological markers in T1D high risk children and relate it to clinical/immunological parameters. Children from ABIS (All Babies in Southeast Sweden) with ≥2 diabetes related autoantibodies were considered at high risk. Age-matched controls and new-onset T1D patients were included. Expression of genes related to immune cell function and different arms of the immune system was assessed in peripheral blood mononuclear cells using PCR array. Risk children had lower TNF and CD45, and although there were few differences between the groups, expression of many genes differed when comparing children with regard to residual insulin secretion. Hence, expression of immune related genes seemed related not only to the autoimmune process but rather to residual β-cell function, which was decreased already during the pre-diabetic phase.
authors: Division of Pediatrics, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden. linda.akerman@liu.se
authors: Linda
authors: L
authors: Akerman
authors: Johnny
authors: J
authors: Ludvigsson
authors: Rosaura
authors: R
authors: Casas
mesh_terms: D000293:Adolescent; D002096:C-Peptide / Q000097:blood* / Q000235:genetics / Q000276:immunology; D002648:Child; D015331:Cohort Studies; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000276:immunology*; D005260:Female; D020869:Gene Expression Profiling; D006680:HLA Antigens / Q000097:blood / Q000276:immunology; D006801:Humans; D017493:Leukocyte Common Antigens / Q000097:blood* / Q000235:genetics / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D010446:Peptide Fragments / Q000097:blood* / Q000235:genetics / Q000276:immunology; D011236:Prediabetic State / Q000097:blood / Q000276:immunology*; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013548:Sweden; D014409:Tumor Necrosis Factor-alpha / Q000097:blood* / Q000235:genetics / Q000276:immunology
chemical_list: D002096:C-Peptide; D006680:HLA Antigens; D010446:Peptide Fragments; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; C076419:tumor necrosis factor (1-26); D017493:Leukocyte Common Antigens; C516769:PTPRC protein, human