title: Isolation, Identification, and Bioactivity of Monoterpenoids and Sesquiterpenoids from the Mycelia of Edible Mushroom Pleurotus cornucopiae.
abstract: Edible mushroom is a profilic source of bioactive metabolites for the development of drugs and nutraceuticals. In this work, four new monoterpenoids (1-4) and one new sesquiterpenoid (6) were isolated from the mycelia of edible mushroom Pleurotus cornucopiae fermented on rice. Their structures were established by nuclear magnetic resonance, mass spectrometry, and circular dichroism (CD) data analysis. Compound 1 possesses an unusual spiro[benzofuran-3,2'-oxiran] skeleton. The absolute configuration of the 6,7-diol moieties in compounds 1, 2, and 6 was assigned using the in situ dimolybdenum CD method. Compounds 1-5, 7, and 8 showed moderate inhibitory activity against nitric oxide production in lipopolysaccaride-activated macrophages, with IC50 values in the range of 60-90 μM. Compounds 6 and 7 also exhibited slight cytotoxicity against HeLa and HepG2 cells.
authors: State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences , 8 Beiertiao, Zhongguancun, Haidian District, Beijing 100190, People's Republic of China.
authors: Shaojuan
authors: S
authors: Wang
authors: Li
authors: L
authors: Bao
authors: Feng
authors: F
authors: Zhao
authors: Quanxin
authors: Q
authors: Wang
authors: Shaojie
authors: S
authors: Li
authors: Jinwei
authors: J
authors: Ren
authors: Li
authors: L
authors: Li
authors: Huaan
authors: H
authors: Wen
authors: Liangdong
authors: L
authors: Guo
authors: Hongwei
authors: H
authors: Liu
mesh_terms: D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D008264:Macrophages / Q000187:drug effects; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D039821:Monoterpenes / Q000737:chemistry* / Q000302:isolation & purification* / Q000633:toxicity; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000378:metabolism; D020076:Pleurotus / Q000737:chemistry*; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity; D014675:Vegetables / Q000737:chemistry*
chemical_list: D039821:Monoterpenes; D012717:Sesquiterpenes; D009569:Nitric Oxide