title: The effect of energy substrates on PHB accumulation of Acidiphilium cryptum DX1-1.
abstract: The effect of glucose and elemental sulfur on the growth and PHB accumulation of Acidiphilium cryptum DX1-1 was investigated. Meanwhile, the differential expressions of 19 genes related with PHB accumulation, sulfur metabolism and carbon fixed in heterotrophy, phytotrophy and mixotrophy were studied by RT-qPCR. The results showed that strain DX1-1 could accumulate PHB with sulfur as the energy substance and atmospheric CO2 as carbon resource. Glucose could improve the growth of strain DX1-1 cultured in medium with sulfur as the energy substance, and almost all the key enzyme-encoding genes related with PHB, sulfur metabolism and carbon fixed were basically up-regulated. PHB polymerase (Arcy_3030), ribulose-bisphosphate carboxylase (Acry_0825), ribulose-phosphate-epimerase (Acry_0022), and cysteine synthase A (Acry_2560) played important role in PHB accumulation, the modified expression of which could influence the PHB yield. With CO2 as carbon resource, the main initial substance of PHB accumulation for strain DX1-1 was acetyl-CoA, instead of acetate with the glucose as the carbon resource. Because of accumulating PHB by fixed atmospheric CO2 while independent of light, A. cryptum DX1-1 may have specifically potential in production of PHB.
authors: School of Environment and Municipal Engineering, Qingdao Technological University, Qingdao, 26600, China. xalcsu@sina.com
authors: Ai-ling
authors: AL
authors: Xu
authors: Jin-lan
authors: JL
authors: Xia
authors: Zhi-wen
authors: ZW
authors: Song
authors: Peng
authors: P
authors: Jiang
authors: Yan
authors: Y
authors: Xia
authors: Min-xi
authors: MX
authors: Wan
authors: Rui-yong
authors: RY
authors: Zhang
authors: Yi
authors: Y
authors: Yang
authors: Ke-ke
authors: KK
authors: Liu
mesh_terms: D041801:Acidiphilium / Q000235:genetics / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D004734:Energy Metabolism; D020869:Gene Expression Profiling; D005947:Glucose / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000378:metabolism*; D053858:Metabolic Networks and Pathways / Q000235:genetics; D011091:Polyesters / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D013455:Sulfur / Q000378:metabolism*; D000094122:Polyhydroxybutyrates
chemical_list: D002245:Carbon Dioxide; D005947:Glucose; D006885:Hydroxybutyrates; D011091:Polyesters; D013455:Sulfur; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates
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