title: The protective effects of beta-casomorphin-7 against glucose -induced renal oxidative stress in vivo and vitro.
abstract: Oxidative stress is implicated in the pathogenesis of diabetic nephropathy. The present study aimed to investigate the effect of β-casomorphin-7 (BCM7) on the oxidative stress occurring in kidney tissue in streptozotocin (STZ)-induced diabetic rats and proximal tubular epithelial cells (NRK-52E) exposure to high glucose (HG) by using biochemical methods. There is a significant decrease in plasma insulin and a significant increase in plasma glucagon in the rats of diabetic group. Oral administration of BCM7 for 30 days to rats with STZ-induced diabetes resulted in a significant increase in serum level of insulin, and a decrease in the level of glucagon. Moreover, rats with STZ-induced diabetes had lower levels of superoxide dismutase (SOD), glutathione peroxidase (GPx) and total antioxidative capacity (T-AOC), higher levels of malondialdehyde (MDA) and hydrogen peroxide (H2O2) in the kidney than that in the control rats. The administration of BCM7 altered the changes of SOD, GPx, T-AOC, MDA and H2O2 in the kidney of diabetic rats. Furthermore, BCM7 alleviated high glucose-induced decreasement in SOD and GPx activity, increasement in MDA contents in the NRK-52E cells. BCM7 ameliorated the changes of angiotensin converting enzyme (ACE) and ACE2 levels in the kidney of diabetic rats and BCM7 lowered the levels of angiotensin (Ang)II in the kidney of diabetic rats and culture medium for cells. Moreover losartan (antagonist of angiotensin II type I receptor) lowered the high glucose-induced oxidative stress in the NRK-52E cells. Our results suggest that administration of BCM7 would alleviate high glucose-induced renal oxidative stress in vivo and in vitro, which may be associated with down regulation of the concentration of Ang II partly.
authors: Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agriculture University, Nanjing, People's Republic of China.
authors: Wei
authors: W
authors: Zhang
authors: Jinfeng
authors: J
authors: Miao
authors: Shanshan
authors: S
authors: Wang
authors: Yuanshu
authors: Y
authors: Zhang
mesh_terms: D000804:Angiotensin II / Q000378:metabolism; D047228:Angiotensin II Type 1 Receptor Blockers / Q000494:pharmacology; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002460:Cell Line; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D003928:Diabetic Nephropathies / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D004723:Endorphins / Q000494:pharmacology*; D004847:Epithelial Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D005934:Glucagon / Q000097:blood; D005979:Glutathione Peroxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000378:metabolism; D007328:Insulin / Q000097:blood; D007527:Isoenzymes / Q000378:metabolism; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D019808:Losartan / Q000494:pharmacology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010446:Peptide Fragments / Q000494:pharmacology*; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D047228:Angiotensin II Type 1 Receptor Blockers; D001786:Blood Glucose; D004723:Endorphins; D007328:Insulin; D007527:Isoenzymes; D010446:Peptide Fragments; D000804:Angiotensin II; D008315:Malondialdehyde; C041128:beta-casomorphin 7; D005934:Glucagon; D006861:Hydrogen Peroxide; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D007703:Peptidyl-Dipeptidase A; D019808:Losartan
references: Methods Enzymol. 1990;186:421-31
references: 2233309
references: Anal Biochem. 1966 Aug;16(2):359-64
references: 6007581
references: Diabetes. 2004 Mar;53(3):762-8
references: 14988262
references: Methods Enzymol. 1984;105:114-21
references: 6727659
references: Electrophoresis. 1988 Jun;9(6):255-62
references: 2466658
references: Peptides. 2012 Aug;36(2):186-91
references: 22687367
references: Free Radic Res. 2011 May;45(5):575-84
references: 21381899
references: Nature. 2001 Dec 13;414(6865):813-20
references: 11742414
references: Peptides. 2010 Sep;31(9):1725-9
references: 20685284
references: Circ Res. 2010 Oct 29;107(9):1058-70
references: 21030723
references: PLoS One. 2012;7(6):e39824
references: 22745830
references: Kidney Int. 2002 Jan;61(1):186-94
references: 11786100
references: Cancer Res. 1984 Nov;44(11):5086-91
references: 6149017
references: Am J Pathol. 2006 Jun;168(6):1808-20
references: 16723697
references: J Clin Biochem Nutr. 2011 Jan;48(1):72-7
references: 21297916
references: Nature. 2001 Dec 13;414(6865):782-7
references: 11742409
references: Free Radic Biol Med. 2003 Jan 15;34(2):186-95
references: 12521600
references: Kidney Int. 2005 May;67(5):1684-91
references: 15840014
references: Diabetologia. 1999 Mar;42(3):292-6
references: 10096780
references: PLoS One. 2012;7(8):e42623
references: 22900035
references: Biochem Biophys Res Commun. 1972 Jan 31;46(2):849-54
references: 4400444
references: IUBMB Life. 2000 Apr;49(4):303-7
references: 10995033
references: Exp Diabetes Res. 2011;2011:521076
references: 22110472
references: Clin Chem. 1997 Jul;43(7):1209-14
references: 9216458
references: Lancet. 1998 Jul 18;352(9123):213-9
references: 9683226
references: Eur J Pharmacol. 1984 Oct 30;106(1):213-4
references: 6529969
references: PLoS One. 2012;7(10):e47360
references: 23077601
references: Diabetes. 2008 Jun;57(6):1446-54
references: 18511445
references: Regul Pept. 2000 Jul 28;91(1-3):21-7
references: 10967199
references: Food Chem Toxicol. 2010 Aug-Sep;48(8-9):2407-12
references: 20570704
references: Mol Med. 2011 Jan-Feb;17(1-2):59-69
references: 20844835
references: Diabetes. 1991 Oct;40(10):1328-34
references: 1834497
references: Eur J Biochem. 1974 Sep 16;47(3):469-74
references: 4215654
references: PLoS One. 2012;7(7):e39739
references: 22808054
references: Oxid Med Cell Longev. 2010 Mar-Apr;3(2):101-8
references: 20716934
references: Hoppe Seylers Z Physiol Chem. 1979 Sep;360(9):1211-6
references: 511110
references: Free Radic Biol Med. 1990;9(6):515-40
references: 2079232
references: Mol Cell Biochem. 2011 Nov;357(1-2):171-9
references: 21625956
references: Methods Enzymol. 1984;105:429-35
references: 6328196
references: Metabolism. 1990 Sep;39(9):971-5
references: 2202888