title: LPS challenge regulates gene expression and tissue localization of a Ciona intestinalis gene through an alternative polyadenylation mechanism.
abstract: A subtractive hybridization strategy for the identification of differentially expressed genes was performed between LPS-challenged and naive Ciona intestinalis. This strategy allowed the characterization of two transcripts (Ci8short and Ci8long) generated by the use of two Alternative Polyadenylation sites. The Ci8long transcript contains a protein domain with relevant homology to several components of the Receptor Transporting Protein (RTP) family not present in the Ci8short mRNA. By means of Real Time PCR and Northern Blot, the Ci8short and Ci8long transcripts showed a different pattern of gene expression with the Ci8short mRNA being strongly activated after LPS injection in the pharynx. In situ hybridization analysis demonstrated that the activation of the APA site also influenced the tissue localization of the Ci8short transcript. This analysis showed that the Ci8long mRNA was expressed in hemocytes meanwhile the Ci8short mRNA was highly transcribed also in vessel endothelial cells and in the epithelium of pharynx. These findings demonstrated that regulation of gene expression based on different polyadenylation sites is an ancestral powerful strategy influencing both the level of expression and tissue distribution of alternative transcripts.
authors: Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche, Palermo, Italy.
authors: Aiti
authors: A
authors: Vizzini
authors: Angela
authors: A
authors: Bonura
authors: Daniela
authors: D
authors: Parrinello
authors: Maria Antonietta
authors: MA
authors: Sanfratello
authors: Valeria
authors: V
authors: Longo
authors: Paolo
authors: P
authors: Colombo
mesh_terms: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002938:Ciona intestinalis / Q000145:classification / Q000235:genetics* / Q000276:immunology / Q000378:metabolism*; D019295:Computational Biology; D018076:DNA, Complementary / Q000737:chemistry; D015870:Gene Expression; D023061:Gene Order; D005796:Genes; D008070:Lipopolysaccharides / Q000276:immunology; D008969:Molecular Sequence Data; D010802:Phylogeny; D026723:Polyadenylation; D021381:Protein Transport; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment
chemical_list: D020413:3' Untranslated Regions; D020121:5' Untranslated Regions; D018076:DNA, Complementary; D008070:Lipopolysaccharides; D011506:Proteins; D012333:RNA, Messenger
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