title: Structure-activity relationships of analogs of 3,4,5-trimethylfuran-2(5H)-one with germination inhibitory activities.
abstract: Smoke-derived butenolide compounds have, in recent years, been shown to be important germination signaling molecules, which also affect seedling growth. The butenolide 3,4,5-trimethylfuran-2(5H)-one was previously isolated from plant-derived smoke and was found to significantly reduce the effect on germination by the highly active promotor karrikinolide (KAR1, 3-methyl-2H-furo[2,3-c]pyran-2-one), another smoke-derived compound. In this study, 11 analogs of 3,4,5-trimethylfuran-2(5H)-one were synthesized and their effect on the germination of light-sensitive 'Grand Rapids' lettuce seeds (Lactua sativa cv. 'Grand Rapids') were evaluated. A concentration series (1mM-1μM) of the analogs were tested alone, or in combination with 0.01μM KAR1. Only two compounds were found to reduce the germination promotory effect of 0.01μM KAR1 in a similar manner as observed with 3,4,5-trimethylfuran-2(5H)-one, with activity ranging from 1mM to 10μM. Four compounds were found to have inhibitory activity at 1mM and 100μM. The retention of activity by some of the analogs may be useful for designing novel compounds with improved activity. Furthermore, understanding the structure-activity relationships of these compounds may be helpful in synthesizing molecular probes that can be used to further investigate the mechanism of action of these compounds in regulating seed germination.
authors: Institute of Organic Chemistry and Biochemistry AS CR, v.v.i., Flemingovo nám. 2, 166 10 Prague 6, Czech Republic.
authors: Martin
authors: M
authors: Pošta
authors: Marnie E
authors: ME
authors: Light
authors: Heino B
authors: HB
authors: Papenfus
authors: Johannes
authors: J
authors: Van Staden
authors: Ladislav
authors: L
authors: Kohout
mesh_terms: D004305:Dose-Response Relationship, Drug; D005663:Furans / Q000138:chemical synthesis / Q000494:pharmacology*; D018525:Germination / Q000187:drug effects*; D018545:Lactuca / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D010937:Plant Growth Regulators / Q000138:chemical synthesis / Q000494:pharmacology*; D011714:Pyrans / Q000494:pharmacology*; D012639:Seeds / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D012906:Smoke / Q000032:analysis; D013329:Structure-Activity Relationship
chemical_list: C548099:3,4,5-trimethylfuran-2(5H)-one; D005663:Furans; D010937:Plant Growth Regulators; D011714:Pyrans; D012906:Smoke; C557869:karrikinolide
keywords: Butenolide; KAR(1); Karrikinolide; Lactuca sativa; Seed germination; Smoke; karrikinolide (3-methyl-2H-furo[2,3-c]pyran-2-one)