title: A new matrix assisted ionization method for the analysis of volatile and nonvolatile compounds by atmospheric probe mass spectrometry.
abstract: Matrix assisted ionization of nonvolatile compounds is shown not to be limited to vacuum conditions and does not require a laser. Simply placing a solution of analyte dissolved with a suitable matrix such as 3-nitrobenzonitrile (3-NBN) or 2,5-dihydroxyacetophenone on a melting point tube and gently heating the dried sample near the ion entrance aperture of a mass spectrometer using a flow of gas produces abundant ions of peptides, small proteins, drugs, and polar lipids. Fundamental studies point to matrix-mediated ionization occurring prior to the entrance aperture of the mass spectrometer. The method is analytically useful, producing peptide mass fingerprints of bovine serum albumin tryptic digest consuming sub-picomoles of sample. Application of 100 fmol of angiotensin I in 3-NBN matrix produces the doubly and triply protonated molecular ions as the most abundant peaks in the mass spectrum. No carryover is observed for samples containing up to 100 pmol of this peptide. A commercial atmospheric samples analysis probe provides a simple method for sample introduction to an atmospheric pressure ion source for analysis of volatile and nonvolatile compounds without using the corona discharge but using sample preparation similar to matrix-assisted laser desorption/ionization.
authors: University of the Sciences, Philadelphia, PA, USA.
authors: Shubhashis
authors: S
authors: Chakrabarty
authors: Vincent S
authors: VS
authors: Pagnotti
authors: Ellen D
authors: ED
authors: Inutan
authors: Sarah
authors: S
authors: Trimpin
authors: Charles N
authors: CN
authors: McEwen
mesh_terms: D000818:Animals; D001274:Atmospheric Pressure; D002417:Cattle; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D008055:Lipids / Q000737:chemistry; D009570:Nitriles; D009578:Nitrobenzenes; D010446:Peptide Fragments / Q000737:chemistry; D010449:Peptide Mapping / Q000379:methods; D004364:Pharmaceutical Preparations / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D014357:Trypsin; D055549:Volatile Organic Compounds / Q000032:analysis
chemical_list: D008055:Lipids; D009570:Nitriles; D009578:Nitrobenzenes; D010446:Peptide Fragments; D004364:Pharmaceutical Preparations; D055549:Volatile Organic Compounds; D012710:Serum Albumin, Bovine; C110438:3-nitrobenzonitrile; D014357:Trypsin
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