title: Is the speed of chronic compression an important factor for chronic spinal cord injury rat model?
abstract: OBJECTIVE
  To evaluate the effect of expansion speed on chronic compressive spinal cord injury in the rat.
  
  
  METHODS
  Thirty-six Sprague-Dawley rats were divided into four groups: a control group, a group receiving compressor in the C5-C6 epidural space with instant compression (group 1), and two other groups receiving water-absorbing polyurethane polymer sheets with two expansion speeds, which reached maximum volume in 2 h (group 2: fast expansion) or 24 h (group 3: slow expansion). A C6 laminectomy was performed in the control group. Neurological function, MRI, large motoneuron number in the ventral horn, and myelin staining intensity in the posterior funiculus were evaluated.
  
  
  RESULTS
  In the instant compression group, compression was confirmed on T2-weighted images by a hypointense signal change in the intramedulla. In the gradual compressive injury groups, large motoneuron number (p<0.001), but not myelin staining intensity, was significantly decreased in both the fast and slow expansion groups compared with the instant compression group. However, there was no difference in Basso Beattie Bresnahan score, cord distortion in T2-weighted image, large motoneuron numbers, or myelin staining between the fast and slow expansion groups.
  
  
  CONCLUSIONS
  Instant spinal cord compression caused acute injury. Gradual expansion compression induced reliable pathology and MRI characteristics consistent with chronic compressive spinal cord injury. The speed of expansion is not a significant problem for establishing a reliable model if the chronic compression is induced by gradual expansion.
authors: Department of Spine Surgery, The First Affiliated Hospital of Sun Yat-Sen University, China. houqinglong@163.com
authors: Hou-Qing
authors: HQ
authors: Long
authors: Guang-Sheng
authors: GS
authors: Li
authors: Er-Jian
authors: EJ
authors: Lin
authors: Wen-Han
authors: WH
authors: Xie
authors: Wen-Li
authors: WL
authors: Chen
authors: Keith Dip-Kei
authors: KD
authors: Luk
authors: Yong
authors: Y
authors: Hu
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D006801:Humans; D009046:Motor Neurons / Q000473:pathology*; D010812:Physical Stimulation / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013117:Spinal Cord Compression / Q000150:complications / Q000473:pathology* / Q000503:physiopathology*; D013119:Spinal Cord Injuries / Q000473:pathology* / Q000503:physiopathology*