title: Analysis of the activity of virus internal ribosome entry site in silkworm Bombyx mori.
abstract: Internal ribosome entry site (IRES) has been widely used in genetic engineering; however, the application in silkworm (Bombyx mori) has hardly been reported. In this study, the biological activity of partial sequence of Encephalomyocarditis virus (EMCV) IRES, Rhopalosiphum padi virus (RhPV) IRES, and the hybrid of IRES of EMCV and RhPV were investigated in Spodoptera frugiperda (Sf9) cell line and silkworm tissues. The hybrid IRES of EMCV and RhPV showed more effective than EMCV IRES or RhPV IRES in promoting downstream gene expression in insect and silkworm. The activities of all IRESs in middle silk gland of silkworm were higher than those in the fat body and posterior silk gland. The hybrid IRES of EMCV and RhPV was integrated into silkworm genome by transgenic technology to test biological activity of IRES. Each of the positive transgenic individuals had significant expression of report gene EGFP. These results suggested that IRES has a potential to be used in the genetic engineering research of silkworm.
authors: College of Animal Sciences, Zhejiang University, Hangzhou 310029, China.
authors: Lupeng
authors: L
authors: Ye
authors: Lanfang
authors: L
authors: Zhuang
authors: Jisheng
authors: J
authors: Li
authors: Zhengying
authors: Z
authors: You
authors: Jianshe
authors: J
authors: Liang
authors: Hao
authors: H
authors: Wei
authors: Jianrong
authors: J
authors: Lin
authors: Boxiong
authors: B
authors: Zhong
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics / Q000821:virology*; D002460:Cell Line; D017931:DNA Primers; D049750:Genome, Insect; D010957:Plasmids; D012270:Ribosomes / Q000378:metabolism*; D018411:Spodoptera / Q000166:cytology
chemical_list: D017931:DNA Primers
keywords: Bombyx mori; genetic engineering; internal ribosome entry site