title: Cdc25B phosphatase participates in maintaining metaphase II arrest in mouse oocytes.
abstract: Cdc25B is an essential regulator for meiotic resumption in mouse oocytes. However, the role of this phosphatase during the later stage of the meiotic cell cycle is not known. In this study, we investigated the role of Cdc25B during metaphase II (MII) arrest in mouse oocytes. Cdc25B was extensively phosphorylated during MII arrest with an increase in the phosphatase activity toward Cdk1. Downregulation of Cdc25B by antibody injection induced the formation of a pronucleus-like structure. Conversely, overexpression of Cdc25B inhibited Ca(2+)-mediated release from MII arrest. Moreover, Cdc25B was immediately dephosphorylated and hence inactivated during MII exit, suggesting that Cdk1 phosphorylation is required to exit from MII arrest. Interestingly, this inactivation occurred prior to cyclin B degradation. Taken together, our data demonstrate that MII arrest in mouse oocytes is tightly regulated not only by the proteolytic degradation of cyclin B but also by dynamic phosphorylation of Cdk1.
authors: Department of Genetic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon 440-746, Korea.
authors: Hyoeun
authors: H
authors: Kang
authors: Seok Cheol
authors: SC
authors: Hwang
authors: Yong Seok
authors: YS
authors: Park
authors: Jeong Su
authors: JS
authors: Oh
mesh_terms: D000818:Animals; D019138:Antibodies, Blocking / Q000008:administration & dosage; D016203:CDC2 Protein Kinase / Q000378:metabolism*; D059447:Cell Cycle Checkpoints / Q000187:drug effects / Q000235:genetics; D002478:Cells, Cultured; D019926:Cyclin B / Q000378:metabolism*; D005260:Female; D008540:Meiosis / Q000187:drug effects / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D009865:Oocytes / Q000502:physiology*; D010766:Phosphorylation / Q000187:drug effects / Q000235:genetics; D059748:Proteolysis / Q000187:drug effects; D019076:Transgenes / Q000235:genetics; D020687:cdc25 Phosphatases / Q000235:genetics / Q000378:metabolism*
chemical_list: D019138:Antibodies, Blocking; D019926:Cyclin B; D016203:CDC2 Protein Kinase; C517006:Cdc25b protein, mouse; D020687:cdc25 Phosphatases
references: Mol Cell Biol. 2001 Jun;21(12):3853-61
references: 11359894
references: Dev Biol. 2008 May 1;317(1):260-9
references: 18367163
references: Mol Biol Cell. 2003 Oct;14(10):4003-14
references: 14517314
references: Proc Natl Acad Sci U S A. 2007 Dec 11;104(50):19867-72
references: 18056802
references: J Cell Sci. 2013 Mar 1;126(Pt 5):1081-5
references: 23345398
references: Science. 2011 Apr 22;332(6028):462-5
references: 21454751
references: PLoS One. 2011 Feb 17;6(2):e14711
references: 21379580
references: Mol Cell Biol. 2011 Apr;31(7):1478-91
references: 21262764
references: Cell Cycle. 2006 Aug;5(15):1687-98
references: 16921258
references: Reproduction. 2005 Dec;130(6):813-23
references: 16322541
references: Am J Physiol. 1996 May;270(5 Pt 1):C1354-61
references: 8967435
references: Curr Opin Cell Biol. 1996 Dec;8(6):795-804
references: 8939679
references: Nat Genet. 2002 Apr;30(4):446-9
references: 11912493
references: Curr Biol. 2003 Feb 18;13(4):264-75
references: 12593792
references: Genes Dev. 2000 Sep 15;14(18):2344-57
references: 10995390
references: Curr Biol. 2005 Sep 20;15(18):1670-6
references: 16169490
references: J Cell Biol. 2010 Jan 25;188(2):199-207
references: 20083600
references: Methods Mol Biol. 2004;281:153-62
references: 15220527
references: J Biol Chem. 1997 Nov 7;272(45):28607-14
references: 9353326
references: PLoS One. 2007 Feb 28;2(2):e247
references: 17327911