title: [Physicochemical properties and antiproliferative activity of recombinant Yersinia pseudotuberculosis L-asparaginase].
abstract: The physicochemical, catalytic, and antiproliferative activity of a recombinant L-asparaginase from Yersinia pseudotuberculosis (YpA) have been studied. The following results were obtained: the K(M) value for L-asparagine is 17 +/- 0.9 microM, the optimal temperature is 60 degrees C, pH is 8.0, pI is 5.4 +/- 0.3, the L-glutaminase activity is no more than 5-6% of the L-asparaginase activity, and the antiproliferative activity on the Fisher L5178y lymphadenosis cell line comprised T/C = 136% (p < 0.001) at a 15% recovery rate. The described characteristic allows one to regard YpA as an antitumor enzyme with biological features similar to the L-asparaginase of E. coli.
authors: V S
authors: VS
authors: Pokrovskiĭ
authors: M V
authors: MV
authors: Pokrovskaia
authors: S S
authors: SS
authors: Aleksandrova
authors: R M
authors: RM
authors: Andrianov
authors: D D
authors: DD
authors: Zhdanov
authors: N M
authors: NM
authors: Omel'ianiuk
authors: E M
authors: EM
authors: Treshchalina
authors: N N
authors: NN
authors: Sokolov
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001215:Asparaginase / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D051379:Mice; D008811:Mice, Inbred DBA; D009374:Neoplasms, Experimental / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D015011:Yersinia pseudotuberculosis / Q000201:enzymology*
chemical_list: D000970:Antineoplastic Agents; D001426:Bacterial Proteins; D001215:Asparaginase