title: Identification of major facilitator transporters involved in cellulase production during lactose culture of Trichoderma reesei PC-3-7.
abstract: Although lactose is a preferred cellulase inducer in the industrial production of cellulase by Trichoderma reesei, the mechanism of induction is not fully understood. Because sugar transporters might be involved at an early step of induction by oligosaccharides, we sought permeases associated with cellulase induction by lactose. Two such MFS sugar transporters in the T. reesei hyper-cellulolytic PC-3-7 strain, an industrial cellulase producer developed in Japan, were identified in a screening for lactose permeases. Disruption of the genes encoding these two transporters resulted in decreased lactose uptake and delayed growth in lactose culture. Further, the deletion strains produced less cellulase when cultivated on lactose. No substantial differences were observed in cellulase production when PC-3-7 was cultivated in cellulose-based medium. The present work provides evidence that these transporters are critical for cellulase production in lactose culture.
authors: Department of Bioengineering, Nagaoka University of Technology, Kamitomioka, Nagaoka, Niigata, Japan.
authors: Juliano de Oliveira
authors: Jde O
authors: Porciuncula
authors: Takanori
authors: T
authors: Furukawa
authors: Yosuke
authors: Y
authors: Shida
authors: Kazuki
authors: K
authors: Mori
authors: Satoshi
authors: S
authors: Kuhara
authors: Yasushi
authors: Y
authors: Morikawa
authors: Wataru
authors: W
authors: Ogasawara
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001692:Biological Transport; D002480:Cellulase / Q000096:biosynthesis*; D002482:Cellulose / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry; D046508:Culture Techniques; D004790:Enzyme Induction / Q000187:drug effects; D007785:Lactose / Q000494:pharmacology*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D010802:Phylogeny; D014242:Trichoderma / Q000187:drug effects / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D026901:Membrane Transport Proteins; D002482:Cellulose; C021499:lactose permease; D002480:Cellulase; D007785:Lactose