title: The octamer is the major form of CENP-A nucleosomes at human centromeres.
abstract: The centromere is the chromosomal locus that ensures fidelity in genome transmission at cell division. Centromere protein A (CENP-A) is a histone H3 variant that specifies centromere location independently of DNA sequence. Conflicting evidence has emerged regarding the histone composition and stoichiometry of CENP-A nucleosomes. Here we show that the predominant form of the CENP-A particle at human centromeres is an octameric nucleosome. CENP-A nucleosomes are very highly phased on α-satellite 171-base-pair monomers at normal centromeres and also display strong positioning at neocentromeres. At either type of functional centromere, CENP-A nucleosomes exhibit similar DNA-wrapping behavior, as do octameric CENP-A nucleosomes reconstituted with recombinant components, having looser DNA termini than those on conventional nucleosomes containing canonical histone H3. Thus, the fundamental unit of the chromatin that epigenetically specifies centromere location in mammals is an octameric nucleosome with loose termini.
authors: Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
authors: Dan
authors: D
authors: Hasson
authors: Tanya
authors: T
authors: Panchenko
authors: Kevan J
authors: KJ
authors: Salimian
authors: Mishah U
authors: MU
authors: Salman
authors: Nikolina
authors: N
authors: Sekulic
authors: Alicia
authors: A
authors: Alonso
authors: Peter E
authors: PE
authors: Warburton
authors: Ben E
authors: BE
authors: Black
mesh_terms: D001324:Autoantigens / Q000032:analysis*; D002503:Centromere / Q000737:chemistry*; D000076247:Centromere Protein A; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis*; D020869:Gene Expression Profiling; D006801:Humans; D008954:Models, Biological; D008958:Models, Molecular; D009707:Nucleosomes / Q000737:chemistry*; D055503:Protein Multimerization
chemical_list: D001324:Autoantigens; C000619700:CENPA protein, human; D000076247:Centromere Protein A; D002868:Chromosomal Proteins, Non-Histone; D009707:Nucleosomes
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