title: A transparent organic transistor structure for bidirectional stimulation and recording of primary neurons.
abstract: Real-time stimulation and recording of neural cell bioelectrical activity could provide an unprecedented insight in understanding the functions of the nervous system, and it is crucial for developing advanced in vitro drug screening approaches. Among organic materials, suitable candidates for cell interfacing can be found that combine long-term biocompatibility and mechanical flexibility. Here, we report on transparent organic cell stimulating and sensing transistors (O-CSTs), which provide bidirectional stimulation and recording of primary neurons. We demonstrate that the device enables depolarization and hyperpolarization of the primary neuron membrane potential. The transparency of the device also allows the optical imaging of the modulation of the neuron bioelectrical activity. The maximal amplitude-to-noise ratio of the extracellular recording achieved by the O-CST device exceeds that of a microelectrode array system on the same neuronal preparation by a factor of 16. Our organic cell stimulating and sensing device paves the way to a new generation of devices for stimulation, manipulation and recording of cell bioelectrical activity in vitro and in vivo.
authors: Consiglio Nazionale delle Ricerche, Istituto per la Sintesi Organica e la Fotoreattività, via Gobetti, 101, 40129 Bologna, Italy. valentina.benfenati@isof.cnr.it
authors: Valentina
authors: V
authors: Benfenati
authors: Stefano
authors: S
authors: Toffanin
authors: Simone
authors: S
authors: Bonetti
authors: Guido
authors: G
authors: Turatti
authors: Assunta
authors: A
authors: Pistone
authors: Michela
authors: M
authors: Chiappalone
authors: Anna
authors: A
authors: Sagnella
authors: Andrea
authors: A
authors: Stefani
authors: Gianluca
authors: G
authors: Generali
authors: Giampiero
authors: G
authors: Ruani
authors: Davide
authors: D
authors: Saguatti
authors: Roberto
authors: R
authors: Zamboni
authors: Michele
authors: M
authors: Muccini
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D002478:Cells, Cultured; D004558:Electric Stimulation / Q000295:instrumentation*; D004567:Electrodes, Implanted; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008839:Microelectrodes; D009474:Neurons / Q000502:physiology*; D009930:Organic Chemicals / Q000737:chemistry; D051381:Rats; D012031:Refractometry / Q000295:instrumentation; D014173:Transistors, Electronic
chemical_list: D009930:Organic Chemicals
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