title: Expressing the Erwinia amylovora type III effector DspA/E in the yeast Saccharomyces cerevisiae strongly alters cellular trafficking.
abstract: Erwinia amylovora is responsible for fire blight, a necrotic disease of apples and pears. E. amylovora relies on a type III secretion system (T3SS) to induce disease on host plants. DspA/E belongs to the AvrE family of type III effector. Effectors of the AvrE family are injected via the T3SS in plant cell and are important to promote bacterial growth following infection and to suppress plant defense responses. Their mode of action in the plant cells is unknown. Here we study the physiological effects induced by dspA/E expression in the yeast Saccharomyces cerevisiae. Expression of dspA/E in the yeast inhibits cell growth. This growth inhibition is associated with perturbations of the actin cytoskeleton and endocytosis.
authors: INRA, UMR217, LIPP, 16 rue Claude Bernard, 75231 Paris cedex 05, France ; UPMC, Université Paris VI, UMR217, LIPP, 16 rue Claude Bernard, 75231 Paris cedex 05, France ; AgroParisTech, UMR217, LIPP, 16 rue Claude Bernard, 75231 Paris cedex 05, France.
authors: Sabrina
authors: S
authors: Siamer
authors: Oriane
authors: O
authors: Patrit
authors: Mathilde
authors: M
authors: Fagard
authors: Naïma
authors: N
authors: Belgareh-Touzé
authors: Marie-Anne
authors: MA
authors: Barny
keywords: AvrE; Cellular traffic; DspA/E; Erwinia amylovora; T3Es, type III effectors; T3SS, type III secretion system; Yeast
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