title: An in vitro comparison of microleakage of two self-etched adhesive and the one-bottle adhesive used in pit and fissure sealant with or without saliva contamination.
abstract: OBJECTIVE
  To minimize the deleterious effects of contaminated enamel on bonding, some authors have advocated the use of adhesive systems under the sealant. The aim of this study was to compare the microleakage of two self-etch and the one-bottle adhesive used in pit and fissure sealant with or without saliva contamination.
  
  
  METHODS
  Sixty extracted premolar teeth were assigned to the six groups. Group 1: Acid +saliva +single bond +sealant, Group 2: Acid +single bond +sealant, Group 3: Saliva +S (3) bond +sealant, Group 4: S (3) bond +sealant, Group 5: Saliva +protect bond +sealant, Group 6: Protect bond +sealant. The teeth were thermocycled and immersed in 2% basic fuchsine dye for 24 h, then sectioned and examined with a stereomicroscope under ×40 magnification. The results were evaluated with Kruskal-Wallis and Dunn tests.
  
  
  RESULTS
  Group 2 has the lower microleakage scores than Groups 4 and 6. Also, Group 6 has the lower microleakage scores than Group 4, and generally an increase in microleakage was observed in each group related to contamination.
  
  
  CONCLUSIONS
  The best technique of sealant therapy in saliva contaminated and non- contaminated condition is the use of acid-etching and bonding agent.
authors: Department of Pediatric Dentistry, School of Dentistry, Ahvaz University of Medical Sciences, Ahvaz, Iran.
authors: Leila
authors: L
authors: Bassir
authors: Mashaallah
authors: M
authors: Khanehmasjedi
authors: Ehsan
authors: E
authors: Nasr
authors: Azita
authors: A
authors: Kaviani
mesh_terms: D000134:Acid Etching, Dental / Q000379:methods; D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry; D004396:Coloring Agents; D003763:Dental Leakage / Q000145:classification*; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D006801:Humans; D055115:Light-Curing of Dental Adhesives / Q000379:methods; D008422:Materials Testing; D010756:Phosphoric Acids / Q000737:chemistry; D010895:Pit and Fissure Sealants / Q000737:chemistry*; D019279:Resin Cements / Q000737:chemistry; D012394:Rosaniline Dyes; D012463:Saliva / Q000502:physiology*; D013499:Surface Properties; D013696:Temperature; D013997:Time Factors
chemical_list: C503075:Clearfil Protect Bond; C505694:Clearfil S3 Bond; D004396:Coloring Agents; D017220:Dentin-Bonding Agents; D010756:Phosphoric Acids; D010895:Pit and Fissure Sealants; D019279:Resin Cements; D012394:Rosaniline Dyes; C115612:single bond; D017438:Bisphenol A-Glycidyl Methacrylate; C025485:basic fuchsin; C030242:phosphoric acid