title: Fluorescent optical fiber sensors for cell viability monitoring.
abstract: A new simple method for non-invasive cell culture viability monitoring based on vital fluorescent stains is introduced, and its efficiency for long-term experiments on cells is demonstrated. In contrast to common methods for cell viability control, which are usually either destructive (like flow-type counters or dead cells coloring and counting), or hardly quantitative like fluorescent microscopy, the method described is automated, does not require the removal of cells from their growth area and is sensitive enough to deal with as low as tens of cells.
authors: SRC Bioclinicum, Ugreshskaya 2-85, Moscow, 115088, Russia.
authors: Ilia
authors: I
authors: Sergachev
authors: Alexander
authors: A
authors: Rusanov
authors: Evgeny
authors: E
authors: Trushkin
authors: Dmitry
authors: D
authors: Sakharov
authors: Uwe
authors: U
authors: Marx
authors: Alexander
authors: A
authors: Tonevitsky
mesh_terms: D015374:Biosensing Techniques; D049109:Cell Proliferation; D002470:Cell Survival; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology*; D005434:Flow Cytometry; D006801:Humans; D015603:Keratinocytes / Q000166:cytology*; D008856:Microscopy, Fluorescence; D055100:Optical Fibers; D012867:Skin / Q000166:cytology*