title: Analytical method validation for the determination of meptyldinocap as 2,4-dinitrooctylphenol metabolite in mango and soil using LC-MS/MS and dissipation study of the fungicide in Indian mango field ecosystem.
abstract: An analytical method for the quantitative determination of meptyldinocap (2,4-DNOPC) as 2,4-dinitrooctylphenyl (2,4-DNOP) in mango and soil was developed as well as validated using liquid chromatography tandem mass spectrometry (LC-MS/MS). The method comprised an extraction with an acetone:methanol:4 N HCl (100:10:5, v/v/v) mixture followed by hydrolytic conversion of parent 2,4-DNOPC to the corresponding phenol metabolite (2,4-DNOP), and cleanup was done by liquid:liquid partition using ethyl acetate. Final quantitation was performed by LC-MS/MS of 2,4-DNOP with negative electrospray ionization using gradient elution. The method was validated at concentrations ranging from 0.025 to 2 μg/g, and the limit of quantification (LOQ) of meptyldinocap in mango and soil samples was 0.025 μg/g. The recovery of meptyldinocap from mango and soil sample was found to be 93-98% spiked at different levels with analyte, and the relative standard deviation for repeatability (RSD(r)) and reproducibility (RSD(R)) were acceptable (2-6%). The method was rugged as evident from a low measurement uncertainty at 0.05 μg/g. In order to evaluate its safety use in India a multilocational field dissipation study on meptyldinocap in mango was conducted by following the proposed analytical method.
authors: Department of Chemistry, Kalyani University, Kalyani-741235, WB, India.
authors: Sudeb
authors: S
authors: Mandal
authors: Bappaditya
authors: B
authors: Kanrar
authors: Saktipada
authors: S
authors: Das
authors: Anjan
authors: A
authors: Bhattacharyya
mesh_terms: D019297:2,4-Dinitrophenol / Q000031:analogs & derivatives* / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D004136:Dinitrobenzenes / Q000032:analysis*; D005659:Fungicides, Industrial / Q000032:analysis*; D007194:India; D057230:Limit of Detection; D031022:Mangifera / Q000737:chemistry*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004136:Dinitrobenzenes; D005659:Fungicides, Industrial; C584656:2,4-dinitro-6-(1-methylheptyl)phenol; C556327:meptyldinocap; D019297:2,4-Dinitrophenol