title: Evidence for Occurrence of Human group B rotavirus in Central India Based on Characterization of NSP2 Gene.
abstract: Human group B rotavirus (HuGBR) was first described as the causative agent of severe gastroenteritis, affecting millions of people in China during 1982-1983. In spite of serological evidences for the presence of HuGBR in many countries of the world, the virus has only been detected from China, Bangladesh and some parts of India. The present study describes a HuGBR (designated as MP-1 isolate) which was confirmed in an adult patient suffering from gastroenteritis in 2008 in Madhya Pradesh, central India. The RNA electrophoresis in polyacrylamide gel (RNA-PAGE) and NSP2 gene based RT-PCR assays and later sequencing was used to confirm the isolate. The nucleotide and deduced amino acid sequences of this HuGBR (MP-1) isolate were analyzed and their relationship with corresponding gene of other Indian, Bangladeshi and Chinese HuGBR and animal group B rotaviruses (AnGBR) was determined. The isolate showed a typical RNA banding pattern of 4:2:2:3 in RNA-PAGE which was indicative of group B rotaviruses (GBR). The sequence comparison of MP-1 isolate with NSP2 gene revealed that MP-1 isolate had 98.6 and 97.7% nucleotide sequence homology and 93.8% amino acid similarity with Bang373 and CAL-1 strains, respectively. The nucleotide and amino acid sequence similarity of MP-1 isolate with one of the Chinese ADRV (WH-1) strain was 92.8 and 92.5%, respectively. While sequence homology with another Chinese strain ADRV (J19) was considerably lower (45.6 and 48.3%, respectively). The percent identity with AnGBRs (porcine and murine) was also lower at nucleotide and amino acid level (66 to 80%). The phylogenetic analysis suggested that MP-1 isolate is closer to Bangladeshi (Bang373) as compared to Indian strain (CAL-1). Our findings indicated that MP-1 isolate might have originated from a common ancestral HuGBR virus but distinct from AnGBR lineage. Occurrence of GBR in other parts of India warrants further epidemiological and molecular studies to develop effective control strategies for GBR infection in adults as well as children.
authors: Division of Virology, IVRI, Mukteswar Campus, Nainital, 263138 Uttarakhand India.
authors: Y P S
authors: YP
authors: Malik
authors: K M
authors: KM
authors: Chandrashekar
authors: K
authors: K
authors: Sharma
authors: Minakshi
authors: M
authors: Prasad
authors: G
authors: G
authors: Prasad
keywords: Group B; NSP2 gene; Rotavirus; Sequence analysis
references: Vet Pathol. 1989 Sep;26(5):376-85
references: 2555957
references: J Clin Microbiol. 1989 Oct;27(10):2180-3
references: 2479654
references: J Clin Virol. 2000 Sep 1;17(3):177-81
references: 10996114
references: J Clin Microbiol. 1982 Sep;16(3):473-7
references: 6182158
references: Lancet. 1984 May 26;1(8387):1139-42
references: 6144874
references: Lancet. 2001 Jan 20;357(9251):198-9
references: 11213100
references: J Med Virol. 2004 Jan;72(1):149-55
references: 14635024
references: Virology. 1993 May;194(1):277-83
references: 8386876
references: Anal Biochem. 1987 Apr;162(1):156-9
references: 2440339
references: J Clin Microbiol. 1991 Mar;29(3):539-43
references: 1709948
references: J Clin Microbiol. 1991 Mar;29(3):519-23
references: 1645368
references: Lancet. 1999 Jan 30;353(9150):380-1
references: 9950453
references: Emerg Infect Dis. 2003 May;9(5):565-72
references: 12737740
references: J Clin Microbiol. 1993 Apr;31(4):917-23
references: 8385152
references: J Clin Virol. 2006 Jul;36(3):222-7
references: 16765641
references: J Clin Microbiol. 1987 Feb;25(2):316-9
references: 3029164
references: J Med Virol. 2004 Dec;74(4):662-7
references: 15484272
references: Epidemiol Infect. 2004 Aug;132(4):745-9
references: 15310177
references: J Med Virol. 2001 Aug;64(4):583-8
references: 11468747
references: Adv Virus Res. 1988;35:193-218
references: 2852892
references: J Clin Microbiol. 2003 May;41(5):2187-90
references: 12734276
references: J Gen Virol. 2008 Oct;89(Pt 10):2622-2629
references: 18796732