title: Antioxidant systems from Pepper (Capsicum annuum L.): involvement in the response to temperature changes in ripe fruits.
abstract: Sweet pepper is susceptible to changes in the environmental conditions, especially temperatures below 15 °C. In this work, two sets of pepper fruits (Capsicum annuum L.) which underwent distinct temperature profiles in planta were investigated. Accordingly, two harvesting times corresponding to each set were established: Harvest 1, whose fruits developed and ripened at 14.9 °C as average temperature; and Harvest 2, with average temperature of 12.4 °C. The oxidative metabolism was analyzed in all fruits. Although total ascorbate content did not vary between Harvests, a shift from the reduced to the oxidized form (dehydroascorbate), accompanied by a higher ascorbate peroxidase activity, was observed in Harvest 2 with respect to Harvest 1. Moreover, a decrease of the ascorbate-generating enzymatic system, the γ-galactono-lactone dehydrogenase, was found at Harvest 2. The activity values of the NADP-dependent dehydrogenases analyzed seem to indicate that a lower NADPH synthesis may occur in fruits which underwent lower temperature conditions. In spite of the important changes observed in the oxidative metabolism in fruits subjected to lower temperature, no oxidative stress appears to occur, as indicated by the lipid peroxidation and protein oxidation profiles. Thus, the antioxidative systems of pepper fruits seem to be involved in the response against temperature changes.
authors: Department of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, CSIC, Apartado 419, Granada E-18080, Spain. josemanuel.palma@eez.csic.es.
authors: Rosa M
authors: RM
authors: Mateos
authors: Ana
authors: A
authors: Jiménez
authors: Paloma
authors: P
authors: Román
authors: Félix
authors: F
authors: Romojaro
authors: Sierra
authors: S
authors: Bacarizo
authors: Marina
authors: M
authors: Leterrier
authors: Manuel
authors: M
authors: Gómez
authors: Francisca
authors: F
authors: Sevilla
authors: Luis A
authors: LA
authors: Del Río
authors: Francisco J
authors: FJ
authors: Corpas
authors: José M
authors: JM
authors: Palma
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D002212:Capsicum / Q000201:enzymology / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D003683:Dehydroascorbic Acid / Q000378:metabolism; D005638:Fruit / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013696:Temperature
chemical_list: D000975:Antioxidants; D010940:Plant Proteins; D017382:Reactive Oxygen Species; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione; D003683:Dehydroascorbic Acid
references: J Exp Bot. 2006;57(8):1735-45
references: 16760420
references: Plant Physiol. 1997 May;114(1):275-284
references: 12223704
references: Physiol Plant. 2009 Feb;135(2):130-9
references: 19055545
references: Int J Food Sci Nutr. 2005 Feb;56(1):45-51
references: 16019314
references: Eur J Biochem. 2001 May;268(9):2678-86
references: 11322889
references: Protoplasma. 2012 Jul;249(3):599-611
references: 22002742
references: Plant Physiol. 1998 Apr;116(4):1195-200
references: 9536035
references: Plant Physiol. 1995 Jul;108(3):1151-60
references: 7630940
references: Clin Sci (Lond). 1993 Apr;84(4):407-12
references: 8482045
references: Plant Cell Environ. 2012 Feb;35(2):281-95
references: 21414013
references: J Agric Food Chem. 2007 Oct 17;55(21):8596-603
references: 17880151
references: J Plant Physiol. 2003 Dec;160(12):1507-16
references: 14717445
references: Plant Physiol. 2008 Jul;147(3):978-84
references: 18612075
references: J Biol Chem. 1969 Nov 25;244(22):6049-55
references: 5389100
references: J Agric Food Chem. 2010 Jun 9;58(11):6621-9
references: 20462192
references: J Agric Food Chem. 1999 Jan;47(1):100-7
references: 10563856
references: J Agric Food Chem. 2000 May;48(5):1713-20
references: 10820084
references: Methods Enzymol. 1978;52:302-10
references: 672633
references: Biochem Soc Trans. 1996 May;24(2):472-8
references: 8736787
references: Anal Biochem. 1971 Nov;44(1):276-87
references: 4943714
references: Free Radic Res. 1999 Dec;31 Suppl:S11-8
references: 10694035
references: Biol Proced Online. 2001 Nov 16;3:19-25
references: 12734582
references: J Agric Food Chem. 2003 Oct 8;51(21):6293-9
references: 14518958
references: Anal Biochem. 1976 May 7;72:248-54
references: 942051
references: Cell. 1994 Nov 18;79(4):583-93
references: 7954825
references: Plant Cell Physiol. 2006 Jul;47(7):984-94
references: 16766574
references: Plant Cell Environ. 2006 Mar;29(3):409-25
references: 17080595
references: Free Radic Res. 2007 Feb;41(2):191-9
references: 17364945
references: Bioessays. 2006 Nov;28(11):1091-101
references: 17041898
references: Plant Physiol. 2011 Jan;155(1):2-18
references: 21205630
references: Trends Plant Sci. 2002 Sep;7(9):405-10
references: 12234732
references: Planta. 1990 Jan;180(2):278-84
references: 24201957
references: Plant Foods Hum Nutr. 2011 Nov;66(4):416-23
references: 21792678
references: Plant Biol (Stuttg). 2009 Jul;11(4):613-24
references: 19538399
references: Methods Enzymol. 1994;233:346-57
references: 8015469
references: Trends Plant Sci. 2010 Jul;15(7):370-4
references: 20605736
references: Plant Cell Environ. 2006 Jul;29(7):1449-59
references: 17080966
references: J Exp Bot. 2007;58(10):2417-27
references: 17545229
references: Annu Rev Plant Biol. 2004;55:373-99
references: 15377225
references: J Exp Bot. 2006;57(8):1603-20
references: 16714307
references: Biochem J. 1998 Mar 1;330 ( Pt 2):777-84
references: 9480890
references: J Exp Bot. 2004 May;55(399):1105-13
references: 15047761
references: J Exp Bot. 2004 Aug;55(403):1623-33
references: 15234991
references: Planta. 1998 Dec;207(2):280-5
references: 9951728
references: J Agric Food Chem. 2010 May 12;58(9):5374-9
references: 20329773
references: Anal Biochem. 1993 Aug 1;212(2):540-6
references: 8214598
references: Phytochemistry. 2002 Nov;61(6):631-5
references: 12423883
references: J Agric Food Chem. 2010 Jun 9;58(11):6951-9
references: 20450196
references: Plant Physiol. 1999 Nov;121(3):921-928
references: 10557241
references: Annu Rev Plant Physiol Plant Mol Biol. 1998 Jun;49:249-279
references: 15012235
references: Plant Cell Environ. 2010 Apr;33(4):453-67
references: 19712065
references: Methods Enzymol. 1984;105:121-6
references: 6727660
references: New Phytol. 2005 Mar;165(3):683-701
references: 15720680
references: Int Rev Cytol. 1995;163:175-247
references: 8522420
references: J Proteomics. 2011 Aug 12;74(8):1230-43
references: 21524723