title: Characterization and optimization of production of exopolysaccharide from Chlamydomonas reinhardtii.
abstract: Chlamydomonas reinhardtii strain RAC was isolated based on its ability to secrete large amount of exopolysaccharide (EPS). The purified EPS had a molecular weight of 2.25×10(5)Da, and showed fibrillar structure with surfaces having sheet-like appearance. Chemical analysis showed the presence of galacturonic acid, ribose, arabinose, xylose, glucose, galactose and rhamnose sugars. The production of EPS was optimized by the classical one-at-a-time approach and Plackett-Burman design, followed by response surface methodology. The resulting response surface model was statistically significant (p<0.5) and predicted maximum EPS production of 628 mg/L. The optimum production medium consisted of CaCl2 - 74, NaNO3 - 422, K2HPO4 - 10 and MgSO4 - 200mg/L with a pH 7. The EPS showed significant antioxidant activity, which can have several industrial applications. This is the first report on characterization and production of EPS from a Chlamydomonas strain isolated from India. Its differences from the earlier reported EPS are discussed.
authors: Biotechnology Division, Institute of Himalayan Bioresource Technology (IHBT), Council of Scientific and Industrial Research (CSIR), Palampur 176061, India. abafana@rediffmail.com
authors: Amit
authors: A
authors: Bafana
mesh_terms: D016825:Chlamydomonas reinhardtii / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D011134:Polysaccharides