title: Dynamics of peripheral blood lymphocyte subpopulations in the acute and subacute phase of Legionnaires' disease.
abstract: OBJECTIVE
  Absolute lymphocytopenia is recognised as an important hallmark of the immune response to severe infection and observed in patients with Legionnaires' disease. To explore the immune response, we studied the dynamics of peripheral blood lymphocyte subpopulations in the acute and subacute phase of LD.
  
  
  RESULTS
  EDTA-anticoagulated blood was obtained from eight patients on the day the diagnosis was made through detection of L. pneumophila serogroup 1 antigen in urine. A second blood sample was obtained in the subacute phase. Multiparametric flow cytometry was used to calculate lymphocyte counts and values for B-cells, T-cells, NK cells, CD4+ and CD8+ T-cells. Expression of activation markers was analysed. The values obtained in the subacute phase were compared with an age and gender matched control group. Absolute lymphocyte count (×10⁹/l, median and range) significantly increased from 0.8 (0.4-1.6) in the acute phase to 1.4 (0.8-3.4) in the subacute phase. B-cell count showed no significant change, while T-cell count (×10⁶/l, median and range) significantly increased in the subacute phase (495 (182-1024) versus 979 (507-2708), p = 0.012) as a result of significant increases in both CD4+ and CD8+ T-cell counts (374 (146-629) versus 763 (400-1507), p = 0.012 and 119 (29-328) versus 224 (107-862), p = 0.012). In the subacute phase of LD, significant increases were observed in absolute counts of activated CD4+ T-cells, naïve CD4+ T-cells and memory CD4+ T-cells. In the CD8+ T-cell compartment, activated CD8+ T-cells, naïve CD8+ T-cell and memory CD8+ T-cells were significantly increased (p<0.05).
  
  
  CONCLUSIONS
  The acute phase of LD is characterized by absolute lymphocytopenia, which recovers in the subacute phase with an increase in absolute T-cells and re-emergence of activated CD4+ and CD8+ T cells. These observations are in line with the suggested role for T-cell activation in the immune response to LD.
authors: Department of Emergency Medicine and Intensive Care, Jeroen Bosch Hospital, 's-Hertogenbosch, The Netherlands. p.de.jager@jbz.nl
authors: Cornelis P C
authors: CP
authors: de Jager
authors: Eugenie F A
authors: EF
authors: Gemen
authors: Jacqueline
authors: J
authors: Leuvenink
authors: Mirrian
authors: M
authors: Hilbink
authors: Robert J F
authors: RJ
authors: Laheij
authors: Tom
authors: T
authors: van der Poll
authors: Peter C
authors: PC
authors: Wever
mesh_terms: D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000378:metabolism; D002097:C-Reactive Protein / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D016952:Legionella pneumophila; D007877:Legionnaires' Disease / Q000097:blood* / Q000150:complications / Q000276:immunology*; D007958:Leukocyte Count; D008213:Lymphocyte Activation / Q000276:immunology; D018655:Lymphocyte Count; D016131:Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D008231:Lymphopenia / Q000097:blood / Q000209:etiology; D008297:Male; D008875:Middle Aged
chemical_list: D015703:Antigens, CD; D002097:C-Reactive Protein
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