title: A bright and responsive europium probe for determination of pH change within the endoplasmic reticulum of living cells.
abstract: A ratiometric Eu(III) complex has been developed that localises selectively within the endoplasmic reticulum of living cells. Careful calibration, using a time-gated spectral imaging microscope, allows the intensity ratio of emission bands and the variation of excited state lifetime to be used for pH determination, with a pKa of 7.15.
authors: Department of Chemistry, Durham University, South Road, Durham, DH1 3LE, UK.
authors: Brian K
authors: BK
authors: McMahon
authors: Robert
authors: R
authors: Pal
authors: David
authors: D
authors: Parker
mesh_terms: D000818:Animals; D004721:Endoplasmic Reticulum / Q000737:chemistry*; D005063:Europium / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents / Q000737:chemistry; D051379:Mice; D041681:NIH 3T3 Cells
chemical_list: D007202:Indicators and Reagents; D005063:Europium