title: Metabolic flux network analysis of fermentative hydrogen production: using Clostridium tyrobutyricum as an example.
abstract: This study applies metabolic flux network analysis (MFA) to evaluate the metabolic flux of fermentative hydrogen production (FHP) with the use of Clostridium tyrobutyricum fed with either glucose or lactate/acetate as substrates. The MFA results suggest that hydraulic retention time (HRT) presents significant impact on hydrogen production from glucose. At HRT between 4 and 18 h, increase of HRT increased hydrogen production but decreased lactate production, while at HRT below 4 h decrease of HRT increased hydrogen production but decreased lactate production. The flux for lactate, butyrate and acetate seemed to affect H₂ production, due presumably to their impacts on the balance of NADH, ferredoxin and ATP. It is suggested that the MFA can be a useful tool to provide valuable information for optimization and design of the fermentative hydrogen production process.
authors: Department of Environmental Engineering, National Cheng Kung University, No. 1, University Road, Tainan 701, Taiwan.
authors: Hai-Hsuan
authors: HH
authors: Cheng
authors: Liang-Ming
authors: LM
authors: Whang
authors: Che-An
authors: CA
authors: Lin
authors: I-Chun
authors: IC
authors: Liu
authors: Chao-Wei
authors: CW
authors: Wu
mesh_terms: D019149:Bioreactors; D048014:Clostridium tyrobutyricum / Q000378:metabolism*; D005285:Fermentation; D006859:Hydrogen / Q000378:metabolism*; D053858:Metabolic Networks and Pathways; D018505:Waste Management / Q000379:methods
chemical_list: D006859:Hydrogen