title: Rapid surface plasmon resonance immunoassay for the determination of deoxynivalenol in wheat, wheat products, and maize-based baby food.
abstract: A rapid screening assay (9 min/sample) has been developed and validated for the detection of deoxynivalenol in durum wheat, wheat products, and maize-based baby foods using an SPR biosensor. Through a single laboratory validation, the limits of detection (LOD) for wheat, wheat-based breakfast cereal, and maize-based baby food were 57, 9, and 6 μg/kg, respectively. Intra-assay and interassay precisions were calculated for each matrix at the maximum and half-maximum European Union regulatory limits and expressed as the coefficient of variation (CV). All CVs fell below 10% with the exception of the between-run CV for breakfast cereal. Recoveries at the concentrations tested ranged from 92 to 115% for all matrices. Action limits of 161, 348, and 1378 μg/kg were calculated for baby food, wheat-based breakfast cereal, and wheat, respectively, and the linear range of the assay was determined as 250-2000 μg/kg.
authors: Institute of Agri-food and Land Use, School of Biological Sciences, David Keir Building, Queen's University of Belfast, Stranmillis Road, Belfast BT9 5AG, Northern Ireland. j.p.meneely@qub.ac.uk
authors: Julie
authors: J
authors: Meneely
authors: Terence
authors: T
authors: Fodey
authors: Laura
authors: L
authors: Armstrong
authors: Michael
authors: M
authors: Sulyok
authors: Rudolf
authors: R
authors: Krska
authors: Christopher
authors: C
authors: Elliott
mesh_terms: D000818:Animals; D005506:Food Contamination / Q000032:analysis; D007118:Immunoassay / Q000379:methods*; D007225:Infant Food / Q000032:analysis*; D051379:Mice; D008807:Mice, Inbred BALB C; D020349:Surface Plasmon Resonance / Q000379:methods*; D014255:Trichothecenes / Q000032:analysis*; D014908:Triticum / Q000737:chemistry*; D003313:Zea mays / Q000737:chemistry*
chemical_list: D014255:Trichothecenes; C007262:deoxynivalenol