title: Multivalent virus-like-particle vaccine protects against classic and variant infectious bursal disease viruses.
abstract: Nucleotide sequences that encode the pVP2 proteins from a variant infectious bursal disease virus (IBDV) strain designated USA08MD34p and a classic IBDV strain designated Mo195 were produced with the use of reverse-transcriptase-polymerase chain reaction (RT-PCR) and cloned into a pGEM-T Easy vector. A nucleotide sequence that encodes the VP3 protein was also produced from the USA08MD34p viral genome with the use of RT-PCR and cloned into a pGEM-T Easy vector. The VP3 and pVP2 clones were inserted into the pVL1393 baculovirus transfer vector and sequenced to confirm their orientation to the promoter and to ensure they contained uninterrupted open reading frames. Recombinant baculoviruses were constructed by transfection in Sf9 cells. Three recombinant baculoviruses were produced and contained the USA08MD34p-VP3, USA08MD34p-pVP2, or Mo195-pVP2 genomic sequences. Virus-like particles (VLPs) were observed with the use of transmission electron microscopy when the USA08MD34p-VP3 baculovirus was co-inoculated into Sf9 cells with either of the pVP2 constructs. VLPs were also observed when the USA08MD34p-pVP2 and Mo195-pVP2 were coexpressed with USA08MD34p-VP3. These multivalent VLPs contained both classic and variant pVP2 molecules. Stability tests demonstrated the VLPs were stable at 4 and 24 C for 8 wk. The USA08MD34p, Mo195, and multivalent VLPs were used to vaccinate chickens. They induced an IBDV-specific antibody response that was detected by enzyme-linked immunosorbent assay (ELISA), and virus-neutralizing antibodies were detected in vitro. Chickens vaccinated with the multivalent VLPs were protected from a virulent variant IBDV strain (V1) and a virulent classic IBDV strain (STC). The results indicate the multivalent VLPs maintained the antigenic integrity of the variant and classic viruses and have the potential to serve as a multivalent vaccine for use in breeder-flock vaccination programs.
authors.affiliation: Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Avenue, Wooster, OH 44691, USA. Jackwood.2@osu.edu
authors.forename: Daral J
authors.initials: DJ
authors.lastname: Jackwood
mesh_terms: D000818:Animals; D016367:Baculoviridae / Q000235:genetics; D018175:Birnaviridae Infections / Q000517:prevention & control / Q000662:veterinary*; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay; D005822:Genetic Vectors / Q000235:genetics; D007243:Infectious bursal disease virus / Q000145:classification / Q000235:genetics / Q000276:immunology*; D046529:Microscopy, Electron, Transmission; D011201:Poultry Diseases / Q000517:prevention & control*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D061987:Sf9 Cells; D018411:Spodoptera / Q000235:genetics; D014162:Transfection; D058425:Vaccines, Virus-Like Particle / Q000276:immunology*; D015678:Viral Structural Proteins / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*; D014771:Virion / Q000235:genetics
chemical_list: C082513:VP2 protein, infectious bursal disease virus; C478244:VP3 protein, infectious bursal disease virus; D058425:Vaccines, Virus-Like Particle; D015678:Viral Structural Proteins; D014765:Viral Vaccines