title: Production of small nano-sized particles by complex formation between polycations and linearized plasmid DNA at a low pH.
abstract: We report on the technical advance of linearized pDNA (pDNA(linear)) above the circular one (pDNA(circ)) for preparation of small-sized DNA/polycation complexes (DPC) at a low pH. Also, the resistance of the DPC formed with pDNA(linear) against poly-L-asparagine indicates that effective ion-pairing occurred between the pDNA(linear) and polycations.
authors.affiliation: Faculty of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita-Ku, Sapporo, Hokkaido 060-0812, Japan.
authors.forename: Ryohei
authors.initials: R
authors.lastname: Togashi
authors.forename: Hidetaka
authors.initials: H
authors.lastname: Akita
authors.forename: Hideyoshi
authors.initials: H
authors.lastname: Harashima
mesh_terms: D004247:DNA / Q000737:chemistry*; D004270:DNA, Circular / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D049329:Nanostructures / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D010957:Plasmids / Q000737:chemistry*; D011073:Polyamines / Q000737:chemistry*; D000071228:Polyelectrolytes
chemical_list: D004247:DNA; D004270:DNA, Circular; D010455:Peptides; D011073:Polyamines; D000071228:Polyelectrolytes; C045164:polyasparagine
keywords: Compaction; Linearized DNA; Plasmid DNA; Polycation; pH