title: Crystal structure of AcrB complexed with linezolid at 3.5 Å resolution.
abstract: AcrB is an inner membrane resistance-nodulation-cell division efflux pump and is part of the AcrAB-TolC tripartite efflux system. We have determined the crystal structure of AcrB with bound Linezolid at a resolution of 3.5 Å. The structure shows that Linezolid binds to the A385/F386 loops of the symmetric trimer of AcrB. A conformational change of a loop in the bottom of the periplasmic cleft is also observed.
authors.affiliation: Los Alamos National Laboratory, Los Alamos, NM 87545, USA. lwhung@lanl.gov
authors.forename: Li-Wei
authors.initials: LW
authors.lastname: Hung
authors.forename: Heung-Bok
authors.initials: HB
authors.lastname: Kim
authors.forename: Satoshi
authors.initials: S
authors.lastname: Murakami
authors.forename: Goutam
authors.initials: G
authors.lastname: Gupta
authors.forename: Chang-Yub
authors.initials: CY
authors.lastname: Kim
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Terwilliger
mesh_terms: D000081:Acetamides / Q000737:chemistry* / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D018360:Crystallography, X-Ray; D024901:Drug Resistance, Multiple, Bacterial; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000378:metabolism; D000069349:Linezolid; D008958:Models, Molecular; D023303:Oxazolidinones / Q000737:chemistry* / Q000378:metabolism; D019897:Periplasm / Q000378:metabolism
chemical_list: D000081:Acetamides; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D023303:Oxazolidinones; D000069349:Linezolid