title: Elucidating changes in interfacial water structure upon protein adsorption.
abstract: A coat to fit the body: Although protein adsorption onto surfaces is a complicated process, sum-frequency generation (SFG) spectroscopy probes the interfacial water structure around a silica surface as a model protein (bovine serum albumin, BSA) adsorbs onto it from solution. At pH 8.0, the attenuated SFG intensity after BSA adsorption indicates that interfacial water molecules are less ordered due to a reduction in surface ξ-potential. The SFG spectrum shows the C-O stretching vibrations subject to stronger ([symbol: see text]≈3200 cm(-1)) and weaker (3200 cm(-1)) hydrogen bonds.
authors.affiliation: Department of Chemistry, Texas A&M University, College Station, TX 77842-3012, USA.
authors.forename: J
authors.initials: J
authors.lastname: Kim
authors.forename: P S
authors.initials: PS
authors.lastname: Cremer
mesh_terms: D000327:Adsorption; D000818:Animals; D002417:Cattle; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D013499:Surface Properties; D014867:Water / Q000737:chemistry*
chemical_list: D014867:Water; D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide