title: Anti-aging effects of co-enzyme Q10 on periodontal tissues.
abstract: Oxidative stress is associated with age-related reactions. The anti-oxidative effects of a reduced form of co-enzyme Q10 (rCoQ10) suppress oxidative stress, which may contribute to the prevention of age-related inflammatory reactions. We examined the effects of topically applied rCoQ10 on periodontal inflammatory reactions in a rat aging model. Male Fischer 344 rats, 2 (n = 6) and 4 mos (n = 18) of age, were used. All of the two-month-old rats and 6 of the four-month-old rats were sacrificed and 12 remaining four-month-old rats received topically applied ointment with or without 1% rCoQ10 on the gingival surface until they reached 6 mos of age. The rats showed an age-dependent increase in circulating oxidative stress. RCoQ10 decreased oxidative DNA damage and tartrate-resistant acid-phosphatase-positive osteoclasts in the periodontal tissue at 6 mos of age as compared with the control. The same conditions lowered gene expression of caspase-1 and interleukin-1β in the periodontal tissue. Furthermore, Nod-like receptor protein 3 inflammasomes were less activated in periodontal tissues from rCoQ10-treated rats as compared with the control rats. Our results suggest that rCoQ10 suppresses age-related inflammatory reactions and osteoclast differentiation by inhibiting oxidative stress.
authors.affiliation: Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Yoneda
authors.forename: T
authors.initials: T
authors.lastname: Tomofuji
authors.forename: D
authors.initials: D
authors.lastname: Ekuni
authors.forename: T
authors.initials: T
authors.lastname: Azuma
authors.forename: Y
authors.initials: Y
authors.lastname: Endo
authors.forename: K
authors.initials: K
authors.lastname: Kasuyama
authors.forename: T
authors.initials: T
authors.lastname: Machida
authors.forename: M
authors.initials: M
authors.lastname: Morita
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000135:Acid Phosphatase / Q000032:analysis; D000199:Actins / Q000187:drug effects; D000367:Age Factors; D000375:Aging / Q000187:drug effects*; D000539:Alveolar Process / Q000187:drug effects / Q000473:pathology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D051017:Apoptosis Regulatory Proteins; D053468:CARD Signaling Adaptor Proteins; D002352:Carrier Proteins; D020170:Caspase 1 / Q000187:drug effects; D003598:Cytoskeletal Proteins / Q000187:drug effects; D004249:DNA Damage / Q000187:drug effects; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000032:analysis / Q000097:blood; D045222:Electron Transport Chain Complex Proteins / Q000494:pharmacology*; D005881:Gingiva / Q000187:drug effects / Q000473:pathology; D058847:Inflammasomes / Q000187:drug effects; D053583:Interleukin-1beta / Q000187:drug effects; D007527:Isoenzymes / Q000032:analysis; D008297:Male; D023421:Models, Animal; D016328:NF-kappa B / Q000187:drug effects; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; D010010:Osteoclasts / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D010519:Periodontium / Q000187:drug effects* / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D011916:Rats, Inbred F344; D018160:Receptors, Cytoplasmic and Nuclear / Q000187:drug effects; D000071681:Tartrate-Resistant Acid Phosphatase; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000097:blood / Q000494:pharmacology; D014815:Vitamins / Q000494:pharmacology*
chemical_list: D000199:Actins; D000975:Antioxidants; D051017:Apoptosis Regulatory Proteins; D053468:CARD Signaling Adaptor Proteins; D002352:Carrier Proteins; D003598:Cytoskeletal Proteins; D045222:Electron Transport Chain Complex Proteins; D058847:Inflammasomes; D053583:Interleukin-1beta; D007527:Isoenzymes; D016328:NF-kappa B; D000071199:NLR Family, Pyrin Domain-Containing 3 Protein; C573346:Nlrp3 protein, rat; C498535:Pycard protein, rat; D018160:Receptors, Cytoplasmic and Nuclear; D014815:Vitamins; D014451:Ubiquinone; D000080242:8-Hydroxy-2'-Deoxyguanosine; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase; D020170:Caspase 1; C024989:coenzyme Q10; D003849:Deoxyguanosine
keywords: aging; coenzyme Q10; inflammasomes; oxidative stress; periodontium; preclinical drug evaluation