title: Isolation and antifungal properties of cyclo(D-Tyr-L-Leu) diketopiperazine isolated from Bacillus sp. associated with rhabditid entomopathogenic nematode.
abstract: Bacillus sp. associated with an entomopathogenic nematode is shown to produce diketopiperazine (DKP) that showed stronger antifungal activity against Colletotrichum gloeosporioides [minimum inhibitory concentration (MIC): 8 μg mL(- 1)] than commercial fungicide oligochitosan (MIC: 125 μg mL(- 1)). DKP identified as cyclo(D-Tyr-L-Leu) was isolated for the first time from a natural source with a d-tyrosine residue. This report also demonstrates for the first time an antifungal property exploration of cyclo(Tyr-Leu) class of dipeptides. The structural elucidation was carried out using 1D, 2D NMR methods and HPLC.
authors.affiliation: a National Institute for Interdisciplinary Science and Technology (NIIST), CSIR , Thiruvananthapuram , 695 019 , Kerala India.
authors.forename: Nishanth
authors.initials: N
authors.lastname: Kumar
authors.forename: Jaggaiah N
authors.initials: JN
authors.lastname: Gorantla
authors.forename: C
authors.initials: C
authors.lastname: Mohandas
authors.forename: Bala
authors.initials: B
authors.lastname: Nambisan
authors.forename: Ravi S
authors.initials: RS
authors.lastname: Lankalapalli
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001407:Bacillus / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D020231:Colletotrichum / Q000187:drug effects*; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D009348:Nematoda / Q000382:microbiology*; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000935:Antifungal Agents; D010456:Peptides, Cyclic; C000589998:cyclo(D-Tyr-L-Leu)diketopiperazine