title: Perineuronal nets protect fast-spiking interneurons against oxidative stress.
abstract: A hallmark of schizophrenia pathophysiology is the dysfunction of cortical inhibitory GABA neurons expressing parvalbumin, which are essential for coordinating neuronal synchrony during various sensory and cognitive tasks. The high metabolic requirements of these fast-spiking cells may render them susceptible to redox dysregulation and oxidative stress. Using mice carrying a genetic redox imbalance, we demonstrate that extracellular perineuronal nets, which constitute a specialized polyanionic matrix enwrapping most of these interneurons as they mature, play a critical role in the protection against oxidative stress. These nets limit the effect of genetically impaired antioxidant systems and/or excessive reactive oxygen species produced by severe environmental insults. We observe an inverse relationship between the robustness of the perineuronal nets around parvalbumin cells and the degree of intracellular oxidative stress they display. Enzymatic degradation of the perineuronal nets renders mature parvalbumin cells and fast rhythmic neuronal synchrony more susceptible to oxidative stress. In parallel, parvalbumin cells enwrapped with mature perineuronal nets are better protected than immature parvalbumin cells surrounded by less-condensed perineuronal nets. Although the perineuronal nets act as a protective shield, they are also themselves sensitive to excess oxidative stress. The protection might therefore reflect a balance between the oxidative burden on perineuronal net degradation and the capacity of the system to maintain the nets. Abnormal perineuronal nets, as observed in the postmortem patient brain, may thus underlie the vulnerability and functional impairment of pivotal inhibitory circuits in schizophrenia.
authors.affiliation: Department of Psychiatry, Centre Hospitalier Universitaire Vaudois and University of Lausanne, 1008 Prilly-Lausanne, Switzerland.
authors.forename: Jan-Harry
authors.initials: JH
authors.lastname: Cabungcal
authors.forename: Pascal
authors.initials: P
authors.lastname: Steullet
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Morishita
authors.forename: Rudolf
authors.initials: R
authors.lastname: Kraftsik
authors.forename: Michel
authors.initials: M
authors.lastname: Cuenod
authors.forename: Takao K
authors.initials: TK
authors.lastname: Hensch
authors.forename: Kim Q
authors.initials: KQ
authors.lastname: Do
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D003433:Crosses, Genetic; D005071:Evoked Potentials / Q000502:physiology; D005109:Extracellular Matrix / Q000378:metabolism* / Q000502:physiology; D005721:Glutamate-Cysteine Ligase / Q000235:genetics; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D007395:Interneurons / Q000378:metabolism* / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008856:Microscopy, Fluorescence; D018384:Oxidative Stress / Q000502:physiology*; D010320:Parvalbumins / Q000378:metabolism; D012559:Schizophrenia / Q000378:metabolism* / Q000503:physiopathology*
chemical_list: D010320:Parvalbumins; C560749:GCLM protein, mouse; D005721:Glutamate-Cysteine Ligase
keywords: critical period; extracellular matrix; glutamate cysteine ligase; glutathione; neuronal synchronization