title: Identification of the Interaction between P-Glycoprotein and Anxa2 in Multidrug-resistant Human Breast Cancer Cells.
abstract: OBJECTIVE
  To explore the interaction of Anxa2 with P-Glycoprotein (P-gp) in the migration and invasion of the multidrug-resistant (MDR) human breast cancer cell line MCF-7/ADR.
  
  
  METHODS
  A pair of short hairpin RNA (shRNA) targeting P-gp was transfected into MCF-7/ADR cells, and monoclonal cell strains were screened. The expression of P-gp was detected by Western blot. Transwell chambers were used to observe the cell migration capacity and invasion ability. The interaction between P-gp and Anxa2 was examined by immunoprecipitation and immunofluorescence confocal microscopy analyses.
  
  
  RESULTS
  P-gp expression was significantly knocked down, and there were notable decreasing trends in the migration and invasion capability of MDR breast cancer cells (P<0.05). There was a close interaction between Anxa2 and P-gp.
  
  
  CONCLUSIONS
  MCF-7/ADR is an MDR human breast cancer cell line with high migration and invasion abilities. The knockdown of P-gp notably impaired the migration and invasion abilities of the tumor cells. The interaction of Anxa2 with P-pg may play an important role in the enhanced invasiveness of MDR human breast cancer cells.
authors.affiliation: Public Laboratory, Key Laboratory of Breast Cancer Prevention and Therapy, Tianjin Medical University, Ministry of Education, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China.
authors.forename: Hai-Chang
authors.initials: HC
authors.lastname: Zhang
authors.forename: Fei
authors.initials: F
authors.lastname: Zhang
authors.forename: Bing
authors.initials: B
authors.lastname: Wu
authors.forename: Jing-Hua
authors.initials: JH
authors.lastname: Han
authors.forename: Wei
authors.initials: W
authors.lastname: Ji
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Rui-Fang
authors.initials: RF
authors.lastname: Niu
keywords: Anxa2; P-glycoprotein; breast neoplasm; drug resistance; multiple; neoplasm metastasis