title: RNA interference-mediated silencing of NANOG leads to reduced proliferation and self-renewal, cell cycle arrest and apoptosis in T-cell acute lymphoblastic leukemia cells via the p53 signaling pathway.
abstract: NANOG is critical for maintaining the self-renewal and proliferative properties of embryonic stem cells. Here we found that cultured T-cell acute lymphoblastic leukemia (T-ALL) cells, as well as human primary T-ALL cells, express a functional variant of NANOG. NANOG mRNA is derived predominantly from a retrogene locus termed NANOGP8. Furthermore, we showed that RNA interference-mediated NANOG knockdown inhibited cell proliferation, reduced self-renewal, promoted apoptosis and arrested the cell cycle through a p53-mediated pathway in leukemic cells. These findings demonstrate the oncogenic potential of this pluripotent gene in human T-ALL cells.
authors.affiliation: Department of Hematology, Nanjing Medical University, Nanjing, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Cao
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Chong
authors.initials: C
authors.lastname: Chen
authors.forename: Chao
authors.initials: C
authors.lastname: Lv
authors.forename: Fanjing
authors.initials: F
authors.lastname: Meng
authors.forename: Lingyu
authors.initials: L
authors.lastname: Zeng
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Li
authors.forename: Qingyun
authors.initials: Q
authors.lastname: Wu
authors.forename: Kai
authors.initials: K
authors.lastname: Zhao
authors.forename: Bin
authors.initials: B
authors.lastname: Pan
authors.forename: Hai
authors.initials: H
authors.lastname: Cheng
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Kailin
authors.initials: K
authors.lastname: Xu
mesh_terms: D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D059447:Cell Cycle Checkpoints; D002454:Cell Differentiation; D049109:Cell Proliferation; D003114:Colony-Forming Units Assay; D020869:Gene Expression Profiling; D018398:Homeodomain Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D000071317:Nanog Homeobox Protein; D020411:Oligonucleotide Array Sequence Analysis; D054218:Precursor T-Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D018398:Homeodomain Proteins; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein; D012333:RNA, Messenger; C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53
keywords: NANOG; T-cell acute lymphoblastic leukemia; p53