title: Proteomic strategies to characterize signaling pathways.
abstract: Cells respond to external stimuli by transducing signals through a series of intracellular molecules and eliciting an appropriate response. The cascade of events through which the signals are transduced include post-translational modifications such as phosphorylation and ubiquitylation in addition to formation of multi-protein complexes. Improvements in biological mass spectrometry and protein/peptide microarray technology have tremendously improved our ability to probe proteins, protein complexes, and signaling pathways in a high-throughput fashion. Today, a single mass spectrometry-based investigation of a signaling pathway has the potential to uncover the large majority of known signaling intermediates painstakingly characterized over decades in addition to discovering a number of novel ones. Here, we discuss various proteomic strategies to characterize signaling pathways and provide protocols for phosphoproteomic analysis.
authors.affiliation: Institute of Bioinformatics, International Technology Park, Bangalore, India.
authors.forename: H C
authors.initials: HC
authors.lastname: Harsha
authors.forename: Sneha M
authors.initials: SM
authors.lastname: Pinto
authors.forename: Akhilesh
authors.initials: A
authors.lastname: Pandey
mesh_terms: D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D009369:Neoplasms / Q000378:metabolism; D010750:Phosphoproteins / Q000737:chemistry / Q000378:metabolism*; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D040901:Proteomics / Q000379:methods*; D015398:Signal Transduction
chemical_list: D010750:Phosphoproteins