title: Evaluating the in vivo Th2 priming potential among common allergens.
abstract: Exposure to allergens, both man-made and from our environment is increasingly associated with the development of significant human health issues such as allergy and asthma. Allergen induced production of the cytokine interleukin (IL-)4 by Th2 cells is central to the pathogenesis of allergic disease (Gavett et al., 1994). The development of the G4 mouse, that expresses green fluorescent protein (GFP) as a surrogate for IL-4 protein expression has made it possible to directly track the immune cells that produce IL-4. By combining a reliable intradermal immunisation technique with the transgenic G4 mouse we have been able to develop a novel & unique in vivo primary Th2 immune response model (PTh2). When allergens relevant to human disease are evaluated using the PTh2 assay a dose dependent hierarchy of allergenicity is revealed with environmental allergens (cockroach, house dust mite) the most potent and food allergens being the least. In addition, the PTh2 assay is extremely sensitive to the immunoregulatory effects of Mycobacterial extracts and immunosuppressive drugs on primary Th2 cell development. Taken together, this assay provides a standardised method for the identification of the structural and functional properties of proteins relevant to allergenicity, and is a powerful screening tool for novel lead compounds that are effective at inhibiting the primary Th2 response in allergic diseases.
authors.affiliation: Malaghan Institute of Medical Research, Box 7060, Wellington 6242, New Zealand.
authors.forename: Mali
authors.initials: M
authors.lastname: Camberis
authors.forename: Melanie
authors.initials: M
authors.lastname: Prout
authors.forename: Shiau-Choot
authors.initials: SC
authors.lastname: Tang
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Forbes-Blom
authors.forename: Marcus
authors.initials: M
authors.lastname: Robinson
authors.forename: Ryan
authors.initials: R
authors.lastname: Kyle
authors.forename: Yasmine
authors.initials: Y
authors.lastname: Belkaid
authors.forename: William
authors.initials: W
authors.lastname: Paul
authors.forename: Graham
authors.initials: G
authors.lastname: Le Gros
mesh_terms: D000485:Allergens / Q000276:immunology*; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D005260:Female; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012867:Skin / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology*
chemical_list: D000485:Allergens; D008070:Lipopolysaccharides
keywords: Allergenicity; CD4 T cell; House dust mite; In vivo Th2 immunoassay; Interleukin-4; eGFP-reporter