title: Six new phenolic glycosides and a new ceramide from the flowers of Wedelia biflora and their cytotoxicity against some cancer cell lines.
abstract: The plant Wedelia biflora has been used in traditional medicine in India and Vietnam to treat various symptoms. However, the chemical composition of its flowers remains mostly unknown. Therefore, we now report the isolation and structural elucidation of six new phenolic glycosides {wedebicoside A - F (1-6)} and one new ceramide [wedebiceramide (9)], together with six known compounds, 1-O-(2',4'-diangeloyloxy-beta-D-fucopyranosyl)-6-hydroxythymol (7), 1-O-[2',4'-diangeloyloxy-3'-(3"-angeloyloxy-beta-D-fucopyranosyl)-beta-D-fucopyransyl]-6-hydroxythymol (8), anhydrosecoisolariciresinol (10), friedeline (11), epifriedelanol (12) and stigmasterol (13) from the flowers of Wedelia biflora. Their structures were established by 1D and 2D NMR spectroscopy, as well as by high resolution ESI-MS analysis and comparison with literature data. The cytotoxic activities against HeLa, MCF-7 and NCI-H460 were evaluated on some purified compounds at the concentration of 100 microg/mL. Compounds 1, 2, 3 and 5 showed strong cytotoxic activities against three surveyed cancer cell lines. Consequently, this study elucidated the phytochemical composition of W. biflora, as well as the potential use of some of the new compounds against some cancers.
authors.affiliation: Department of Basic Science, University of Medicine and Pharmacy, Ho Chi Minh City, 217 Hong Bang Str., Dist. 5, Ho Chi Minh City, Vietnam.
authors.forename: Nguyen T H
authors.initials: NT
authors.lastname: Thu
authors.forename: Le T
authors.initials: le T
authors.lastname: Ha
authors.forename: Vo T
authors.initials: VT
authors.lastname: Nga
authors.forename: Pham N K
authors.initials: PN
authors.lastname: Tuyen
authors.forename: Ton T
authors.initials: TT
authors.lastname: Quang
authors.forename: Forde-Riddick
authors.initials: FR
authors.lastname: Daniellee
authors.forename: Pratt
authors.initials: P
authors.lastname: Lawrence
authors.forename: Nguyen K P
authors.initials: NK
authors.lastname: Phung
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002518:Ceramides / Q000737:chemistry* / Q000494:pharmacology*; D035264:Flowers / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry* / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D031209:Wedelia / Q000737:chemistry*
chemical_list: D002518:Ceramides; D006027:Glycosides