title: Comparative evaluation of agar dilution and broth microdilution methods for antibiotic susceptibility testing of Helicobacter cinaedi.
abstract: The aim of this study was to establish a broth microdilution method for antimicrobial susceptibility testing of Helicobacter cinaedi and to assess the prevalence and mechanisms of fluoroquinolone resistance in Japanese clinical isolates. A broth microdilution method using modified Levinthal broth was developed and compared with the agar dilution method for testing susceptibility to ampicillin, gentamicin, tetracycline and ciprofloxacin. The minimum inhibitory concentrations obtained by these two methods were almost the same for all the antibiotics tested, demonstrating the broth microdilution method to be a suitable and reliable technique for antimicrobial susceptibility testing. A broth microdilution method for antimicrobial susceptibility test for H. cinaedi was established. This method is expected to help improve treatment.
authors.affiliation: Department of Microbiology, School of Pharmacy, Aichi Gakuin University, 1-100 Kusumoto-cho, Nagoya 464-8650, Japan. jtomida@dpc.agu.ac.jp
authors.forename: Junko
authors.initials: J
authors.lastname: Tomida
authors.forename: Ayako
authors.initials: A
authors.lastname: Oumi
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Okamoto
authors.forename: Yuji
authors.initials: Y
authors.lastname: Morita
authors.forename: Akihiko
authors.initials: A
authors.lastname: Okayama
authors.forename: Naoaki
authors.initials: N
authors.lastname: Misawa
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Hayashi
authors.forename: Takaaki
authors.initials: T
authors.lastname: Akaike
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kawamura
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003470:Culture Media / Q000737:chemistry; D016998:Helicobacter / Q000187:drug effects* / Q000302:isolation & purification; D016481:Helicobacter Infections / Q000382:microbiology; D006801:Humans; D007564:Japan; D008826:Microbial Sensitivity Tests / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media