title: Assaying plant phosphatidic acid phosphatase activity.
abstract: Phosphatidic acid phosphatase (PAP; EC 3.1.3.4) catalyzes the dephosphorylation of phosphatidic acid (PA) to produce diacylglycerol (DAG) and inorganic phosphate. In seed plants, PA plays pivotal roles both as a precursor to membrane lipids and as a signaling molecule. As more information on the roles of PAP in plants becomes available and the importance of PAP is revealed, protocols for assaying plant PAP activity are of interest to an increasing audience. This chapter describes procedures to assay plant PAP activity that are based on recent publications.
authors.affiliation: Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, ROC.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Nakamura
mesh_terms: D002855:Chromatography, Thin Layer; D057075:Enzyme Assays / Q000379:methods*; D004926:Escherichia coli / Q000378:metabolism; D005816:Genetic Complementation Test; D007091:Image Processing, Computer-Assisted; D009154:Mutation / Q000235:genetics; D010711:Phosphatidate Phosphatase / Q000378:metabolism*; D010944:Plants / Q000201:enzymology*; D011994:Recombinant Proteins / Q000302:isolation & purification; D012441:Saccharomyces cerevisiae / Q000235:genetics; D013223:Statistics as Topic
chemical_list: D011994:Recombinant Proteins; D010711:Phosphatidate Phosphatase