title: Fluorescence in situ hybridization (FISH) as an aid for the diagnosis of graft-versus-host disease in two multivisceral organ transplant patients.
abstract: We herein describe 2 cases of adult multivisceral transplant patients who developed graft-versus-host disease manifesting predominantly as lichenoid skin papules and plaques. The diagnosis was supported by histopathology but ultimately corroborated by the utilization of the fluorescence in situ hybridization (FISH) technique using X and Y chromosome probes on unstained biopsy slides. In both cases, FISH revealed a high percentage of donor-derived cells as part of the inflammatory infiltrate in the skin biopsy. This report adds to the previous publications showing the utility of FISH in corroborating the diagnosis of graft-versus-host disease in transplant patients with unmatched sex donor.
authors.affiliation: Department of Dermatology, Indiana University School of Medicine, Indianapolis, IN, USA. cmachado@iupui.edu
authors.forename: Christiane
authors.initials: C
authors.lastname: Machado
authors.forename: Rachael
authors.initials: R
authors.lastname: Teo
authors.forename: Nico
authors.initials: N
authors.lastname: Mousdicas
authors.forename: Marco A
authors.initials: MA
authors.lastname: Lacerda
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Vianna
authors.forename: Gail H
authors.initials: GH
authors.lastname: Vance
authors.forename: Ryan
authors.initials: R
authors.lastname: Stohler
authors.forename: Peter
authors.initials: P
authors.lastname: Ng
authors.forename: Simon J P
authors.initials: SJ
authors.lastname: Warren
mesh_terms: D000328:Adult; D000368:Aged; D001706:Biopsy; D041321:Chromosomes, Human, X; D041322:Chromosomes, Human, Y; D015342:DNA Probes; D017809:Fatal Outcome; D005260:Female; D005819:Genetic Markers; D005820:Genetic Testing / Q000379:methods*; D006086:Graft vs Host Disease / Q000175:diagnosis* / Q000188:drug therapy / Q000235:genetics* / Q000276:immunology / Q000473:pathology; D006801:Humans; D007166:Immunosuppressive Agents / Q000627:therapeutic use; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D016377:Organ Transplantation / Q000009:adverse effects*; D011237:Predictive Value of Tests; D012867:Skin / Q000473:pathology; D016896:Treatment Outcome
chemical_list: D015342:DNA Probes; D005819:Genetic Markers; D007166:Immunosuppressive Agents