title: A universally applicable and rapid method for measuring the growth of streptomyces and other filamentous microorganisms by methylene blue adsorption-desorption.
abstract: Quantitative assessment of growth of filamentous microorganisms, such as streptomycetes, is generally restricted to determination of dry weight. Here, we describe a straightforward methylene blue-based sorption assay to monitor microbial growth quantitatively, simply, and rapidly. The assay is equally applicable to unicellular and filamentous bacterial and eukaryotic microorganisms.
authors.affiliation: Institute for Biology/Microbiology, Martin Luther University Halle-Wittenberg, Halle, Germany.
authors.forename: Marco
authors.initials: M
authors.lastname: Fischer
authors.forename: R Gary
authors.initials: RG
authors.lastname: Sawers
mesh_terms: D000327:Adsorption; D001419:Bacteria / Q000254:growth & development; D001431:Bacteriological Techniques / Q000379:methods*; D056890:Eukaryota / Q000254:growth & development; D008751:Methylene Blue / Q000378:metabolism*; D013302:Streptomyces / Q000254:growth & development* / Q000378:metabolism
chemical_list: D008751:Methylene Blue