title: Immunocytochemical expression of 3beta- and 17beta-hydroxysteroid dehydrogenase in bitch ovaries exposed to low doses of zearalenone.
abstract: Companion animals, including bitches, are exposed to intoxication with zearalenone (ZEN) present in feed, and the above may lead to reproductive disorders which are pften noted in veterinary practice. The aim of this study was to determine the effect of long-term and low-dose ZEN intoxication on the immunoexpression (optical density) of 3beta-hydroxysteroid dehydrogenase (3beta-HSD) and 17beta-hydroxysteroid dehydrogenase (17beta-HSD) in the ovaries of pre-pubertal bitches. The experiment involved 30 clinically healthy, immature Beagle bitches aged approximately 70 days with initial average body weight of 8 kg. The animals were randomly divided into two experimental groups (EI and EII) and a control group (C; n = 10 in all groups). Group EI was administered 50 microg ZEN/kg BW per os over a period of 42 days, group EII received 75 microg ZEN/kg BW per os for 42 days, and group C was administered placebo per os over a period of 42 days. Weighed amounts of the analyzed mycotoxin were administered every morning in gel capsules before feeding. The animals were subjected to ovariohysterectomy at the end of the experiment. Immunocytochemical analyses were performed to localize 3beta-HSD and 17beta-HSD enzymes and determine their optical density. The results revealed that changes in the immunoexpression of the above enzymes were inversely proportional to the applied dose of ZEN substrate.
authors.affiliation: Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland. mgaja@uwm.edu.pl
authors.forename: M
authors.initials: M
authors.lastname: Gajecka
authors.forename: I
authors.initials: I
authors.lastname: Otrocka-Domagała
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D004285:Dogs; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006913:Hydroxysteroid Dehydrogenases / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry / Q000662:veterinary*; D010053:Ovary / Q000187:drug effects* / Q000201:enzymology
chemical_list: D015096:3-Hydroxysteroid Dehydrogenases; D006913:Hydroxysteroid Dehydrogenases; C035507:3(17)-hydroxysteroid dehydrogenase