title: The multifunctional protein YdiV represses P fimbria-mediated adherence in uropathogenic Escherichia coli.
abstract: YdiV, a degenerate EAL domain protein, represses motility by interacting with FlhD to abolish FlhDC interaction with DNA. Here, we demonstrate that deletion of ydiV dysregulates coordinate control of motility and adherence by increasing adherence of Escherichia coli CFT073 to a bladder epithelial cell line by specifically increasing production of P fimbriae. Interestingly, only one of the two P fimbrial operons, pap_2, present in the genome of E. coli CFT073 was upregulated. This derepression of the pap_2 operon is abolished following deletion of either cya or crp, demonstrating cyclic AMP (cAMP)-dependent activation of the P fimbrial operon. However, the absence of YdiV does not affect the gene expression of cya and crp, and loss of SdiA in the ydiV mutant does not affect the derepression of the pap_2 operon, suggesting that YdiV control of adherence acts in response to cAMP levels. Deletion of ydiV increases motility by increasing expression of fliA, suggesting that in E. coli CFT073, YdiV regulates motility by the same mechanism as that described previously for commensal E. coli strains. Furthermore, analysis of site-directed mutations found two putative Mg(2+)-binding residues of four conserved YdiV residues (E29 and Q219) that were involved in regulation of motility and FliC production, while two conserved c-di-GMP-binding residues (D156 and D165) only affected motility. None of the four conserved YdiV residues appeared to affect regulation of adherence. Therefore, we propose a model in which a degenerate EAL, YdiV, utilizes different domains to regulate motility through interaction with FlhD and adherence to epithelial cells through cAMP-dependent effects on the pap_2 promoter.
authors.affiliation: Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA.
authors.forename: Rachel R
authors.initials: RR
authors.lastname: Spurbeck
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Alteri
authors.forename: Stephanie D
authors.initials: SD
authors.lastname: Himpsl
authors.forename: Harry L T
authors.initials: HL
authors.lastname: Mobley
mesh_terms: D001422:Bacterial Adhesion; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D000242:Cyclic AMP / Q000378:metabolism; D004847:Epithelial Cells / Q000382:microbiology; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D010861:Fimbriae, Bacterial / Q000235:genetics / Q000502:physiology*; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D008124:Locomotion; D009876:Operon; D015534:Trans-Activators / Q000378:metabolism; D056689:Uropathogenic Escherichia coli / Q000235:genetics / Q000502:physiology*
chemical_list: D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D015534:Trans-Activators; C577842:YdiV protein, E coli; C090553:flhD protein, E coli; D000242:Cyclic AMP