title: Identification of interferon-γ-inducible-lysosomal thiol reductase (GILT) gene from Mefugu (Takifugu obscures) and its immune response to LPS challenge.
abstract: Interferon-γ-inducible-lysosomal thiol reductase (GILT) plays a key role in the processing and presentation of MHC class II-restricted antigen (Ag) by catalyzing disulfide bond reduction. In this study, a Mefugu cDNA (ToGILT) encodes a deduced protein of 242 amino acids with a putative molecular weight of 28.6 kDa. It contains typical features of GILT proteins including the signature sequence CQHGX2ECX2NX4C, CXXC motif and other five cysteines. Genomic analysis revealed that ToGILT gene exhibited a similar exon-intron organization to human and mouse GILT. Phylogenetic analysis showed that ToGILT derived from a common ancestor with other vertebrate GILT proteins. The ToGILT mRNA was expressed in a tissue-specific manner and obviously up-regulated in spleen and kidney after LPS induction. These results suggest that ToGILT may be involved in the immune response to bacteria challenge in Takifugu obscurus.
authors.affiliation: Jiangsu Province Key Laboratory for Molecular and Medical Biotechnology, Life Sciences College, Nanjing Normal University, Nanjing 210023, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Liu
authors.forename: Hongxin
authors.initials: H
authors.lastname: Ai
authors.forename: Wen
authors.initials: W
authors.lastname: Xiao
authors.forename: Yuefen
authors.initials: Y
authors.lastname: Shen
authors.forename: Yang
authors.initials: Y
authors.lastname: Shen
authors.forename: Xianwei
authors.initials: X
authors.lastname: Cui
authors.forename: Shuangquan
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D003001:Cloning, Molecular; D029941:Fish Proteins / Q000145:classification / Q000235:genetics / Q000276:immunology*; D020869:Gene Expression Profiling; D015723:Gene Library; D008070:Lipopolysaccharides / Q000276:immunology*; D008969:Molecular Sequence Data; D010088:Oxidoreductases / Q000378:metabolism; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000145:classification / Q000235:genetics / Q000276:immunology*; D010802:Phylogeny; D011994:Recombinant Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013438:Sulfhydryl Compounds / Q000378:metabolism; D023741:Takifugu / Q000235:genetics / Q000276:immunology*
chemical_list: D029941:Fish Proteins; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D013438:Sulfhydryl Compounds; D010088:Oxidoreductases; D050862:Oxidoreductases Acting on Sulfur Group Donors
keywords: Lipopolysaccharide (LPS); Phylogenetic analysis; Real-time PCR; Takifugu obscurus; Thiol reductase activity; ToGILT