title: Theoretical and experimental SHG angular intensity patterns from healthy and proteolysed muscles.
abstract: SHG angular intensity pattern (SHG-AIP) of healthy and proteolysed muscle tissues are simulated and imaged here for the first time to our knowledge. The role of the spatial distribution of second-order nonlinear emitters on SHG-AIP is highlighted. SHG-AIP with two symmetrical spots is found to be a signature of healthy muscle whereas SHG-AIP with one centered spot in pathological mdx muscle is found to be a signature of myofibrillar disorder. We also show that SHG-AIP provides information on the three-dimensional structural organization of myofibrils in physiological and proteolysed muscle. Our results open an avenue for future studies aimed at unraveling more complex physiological and pathological fibrillar tissues organization.
authors.affiliation: Institut de Physique de Rennes, Département d' Optique, UMR UR1-Centre National de la Recherche Scientifique 6251, Université de Rennes 1, Rennes, France. denis.rouede@univ-rennes1.fr
authors.forename: Denis
authors.initials: D
authors.lastname: Rouède
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Bellanger
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Schaub
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Recher
authors.forename: François
authors.initials: F
authors.lastname: Tiaho
mesh_terms: D000818:Animals; D018613:Microscopy, Confocal; D008954:Models, Biological; D018482:Muscle, Skeletal / Q000378:metabolism / Q000648:ultrastructure*; D059748:Proteolysis; D057109:Scanning Laser Polarimetry; D014982:Xenopus laevis