title: Direct colposcopic vision used with the LLETZ procedure for optimal treatment of CIN: results of joint cohort studies.
abstract: OBJECTIVE
  To assess the value of direct colposcopic vision (DCV) for optimizing large loop excision of the transformation zone (LLETZ) for the treatment of cervical intraepithelial neoplasia (CIN).
  
  
  METHODS
  Data from 648 patients who underwent excisional procedures for CIN and were included in two previously published cohort studies were retrospectively reviewed. Women who had a LLETZ were included for analysis (n = 436). Margin status, surgical specimen dimensions and volume were analysed according to the use of colposcopy during procedure.
  
  
  RESULTS
  Compared to LLETZ guided by previous colposcopy report only, and to LLETZ performed immediately after colposcopy, DCV allowed for a significantly higher rate of clear margins: 33 (52.4 %), 104 (68.0 %) and 142 (84.5 %), respectively (p < 0.001). It also allowed for a significantly higher probability of achieving both negative margins and depth of specimen <10 mm: 10 (15.9 %) cases, 47 (30.7 %) cases and 125 (74.4 %) cases, respectively (p < 0.001). In multivariate analysis, when compared with the use of previous colposcopy report or with colposcopy immediately before the LLETZ, DCV allowed for a significantly higher probability of negative margins (AOR: 4.61; 95 % CI: 2.37-8.99 and AOR: 2.55; 95 % CI: 1.47-4.41), combined negative margins and depth <75th percentile (AOR: 3.67; 95 % CI: 1.97-6.86 and AOR: 3.05; 95 % CI: 1.91-4.87) and combined negative margins and volume <75th percentile (AOR: 12.96; 95 % CI: 5.99-28.05 and AOR: 6.16; 95 % CI: 3.75-10.14), respectively.
  
  
  CONCLUSIONS
  When used with the LLETZ procedure, DCV allows for optimal outcomes in terms of negative resection margins, and minimized depth and volume of the excised specimen; and should therefore be recommended.
authors.affiliation: Department of Obstetrics and Gynaecology, Assistance Publique des Hôpitaux de Marseille (APHM), Hôpital Nord, Chemin des Bourrelly, 13015, Marseille Cedex 20, France, xcarco@free.fr.
authors.forename: Xavier
authors.initials: X
authors.lastname: Carcopino
authors.forename: Julien
authors.initials: J
authors.lastname: Mancini
authors.forename: Colette
authors.initials: C
authors.lastname: Charpin
authors.forename: Céline
authors.initials: C
authors.lastname: Grisot
authors.forename: Joan Annette
authors.initials: JA
authors.lastname: Maycock
authors.forename: Gilles
authors.initials: G
authors.lastname: Houvenaeghel
authors.forename: Aubert
authors.initials: A
authors.lastname: Agostini
authors.forename: Léon
authors.initials: L
authors.lastname: Boubli
authors.forename: Walter
authors.initials: W
authors.lastname: Prendiville
mesh_terms: D000328:Adult; D000367:Age Factors; D003127:Colposcopy; D005260:Female; D006801:Humans; D008875:Middle Aged; D018365:Neoplasm, Residual; D012189:Retrospective Studies; D013997:Time Factors; D002583:Uterine Cervical Neoplasms / Q000473:pathology / Q000601:surgery*; D002578:Uterine Cervical Dysplasia / Q000473:pathology / Q000601:surgery*