title: WAVE1 gene silencing via RNA interference reduces ovarian cancer cell invasion, migration and proliferation.
abstract: OBJECTIVE
  Wiskott-Aldrich syndrome protein family verprolin-homologous protein 1 (WAVE1) has been implicated in cancer cell migration and invasion. We have previously shown that the overexpression of WAVE1 in epithelial ovarian cancer (EOC) tissues is associated with a poor prognosis. However, the mechanism of WAVE1 regulating the malignant behaviors in EOC remains unclear.
  
  
  METHODS
  In the present study, we knocked down WAVE1 expression in SKOV3 and OVCAR-3 cells through RNA interference to detect the cell biology and molecular biology changes. Moreover, western-blot was used to investigate the underlying mechanism of WAVE1 regulating the proliferative and invasive malignant behaviors in ovarian cancer cells.
  
  
  RESULTS
  The down-regulation of WAVE1 had a significant effect on cell morphological changes. WAVE1 silencing decreased cell migration, cell invasion, cell adhesion, colony formation and cell proliferation in vitro. In addition, we found that down-regulation of WAVE1 inhibited malignant behaviors in vivo. Furthermore, our study also indicated that the PI3K/AKT and p38MAPK signaling pathways might contribute to WAVE1 promotion of ovarian cancer cell proliferation, migration, and invasion.
  
  
  CONCLUSIONS
  WAVE1 might promote the proliferative and invasive malignant behaviors through the activation of the PI3K/AKT and p38MAPK signaling pathways in EOC.
authors.affiliation: Department of Obsterics and Gynecology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Suiyang
authors.initials: S
authors.lastname: Zhou
authors.forename: Liangdan
authors.initials: L
authors.lastname: Tang
authors.forename: Liyuan
authors.initials: L
authors.lastname: Shen
authors.forename: Lin
authors.initials: L
authors.lastname: Xiao
authors.forename: Zhaoning
authors.initials: Z
authors.lastname: Duan
authors.forename: Li
authors.initials: L
authors.lastname: Jia
authors.forename: Yi
authors.initials: Y
authors.lastname: Cao
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Mu
mesh_terms: D000818:Animals; D000077216:Carcinoma, Ovarian Epithelial; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D020868:Gene Silencing; D006801:Humans; D051379:Mice; D009361:Neoplasm Invasiveness; D009375:Neoplasms, Glandular and Epithelial / Q000473:pathology*; D010051:Ovarian Neoplasms / Q000473:pathology*; D019869:Phosphatidylinositol 3-Kinases / Q000502:physiology; D051057:Proto-Oncogene Proteins c-akt / Q000502:physiology; D034622:RNA Interference; D051300:Wiskott-Aldrich Syndrome Protein Family / Q000235:genetics / Q000502:physiology*; D048051:p38 Mitogen-Activated Protein Kinases / Q000502:physiology
chemical_list: C114864:WASF1 protein, human; D051300:Wiskott-Aldrich Syndrome Protein Family; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D048051:p38 Mitogen-Activated Protein Kinases
keywords: Invasion; Ovarian cancer; Proliferation; RNA interference; WAVE1