title: Biological and Molecular Properties of Dengue 2 Strains Isolated during the DHF/DSS Cuban Epidemic, 1981.
abstract: To study some biological and molecular properties of nine DENV-2 strains isolated during the 1981 Cuban epidemic, temperature sensitivity, viral plaque size, the kinetic of virus replication in newborn mice inoculated by intracerebral route, the influence of pH medium on virus-cell attachment phase and the restriction enzyme pattern were studied. Strains were classified in two patterns according to temperature sensitivity, plaque size, and virus replication in mouse brain and cell culture and restriction enzymatic pattern the changes observed differentiate clearly the strains isolated at the beginning and at the end of the epidemic suggesting that viruses with different characteristics circulated.
authors.affiliation: Department of Virology, PAHO/WHO Collaborating Centre for the Study of Dengue and its Vector, Institute of Tropical Medicine "Pedro Kourí", Havana City, Cuba.
authors.forename: Lester
authors.initials: L
authors.lastname: González
authors.forename: Mayling
authors.initials: M
authors.lastname: Alvarez
authors.forename: Delfina
authors.initials: D
authors.lastname: Del Rosario
authors.forename: Alequis
authors.initials: A
authors.lastname: Pavón
authors.forename: Irina
authors.initials: I
authors.lastname: Prado
authors.forename: Joel
authors.initials: J
authors.lastname: Díaz
authors.forename: Luis
authors.initials: L
authors.lastname: Morier
authors.forename: María G
authors.initials: MG
authors.lastname: Guzmán
keywords: 1981; Cuba; DF; DHF; biological properties; dengue; dengue fever; dengue hemorrhagic fever