title: Different kinetics of circulating antibody-secreting cell responses after primary and booster oral immunizations: a tool for assessing immunological memory.
abstract: We show that the kinetics of circulating IgA as well as IgG antibody-secreting cell (ASC) responses differs considerably after primary and booster vaccination with the oral cholera vaccine Dukoral(®), as determined by the antibody in lymphocyte supernatant (ALS) as well as ELISPOT methods. Thus, whereas the antitoxin ASC responses did not peak until 7-9 days after primary vaccination, peak responses to a second dose given after two weeks, or a single booster dose given 6 months to 14 years later, were recorded already after 4-5 days and then rapidly declined. Our results indicate that many previous studies reporting ASC results 7-10 days after repeated immunization may have substantially underestimated the magnitudes of the responses. The results also suggest that detection of peak ASC responses at an early time point after booster immunization can be used as a simple tool to assess immunological memory.
authors.affiliation: Gothenburg University Vaccine Research Institute (GUVAX), Department of Microbiology and Immunology, University of Gothenburg, Sweden. susannah.leach@microbio.gu.se
authors.forename: Susannah
authors.initials: S
authors.lastname: Leach
authors.forename: Anna
authors.initials: A
authors.lastname: Lundgren
authors.forename: Ann-Mari
authors.initials: AM
authors.lastname: Svennerholm
mesh_terms: D000328:Adult; D000921:Antibody-Producing Cells / Q000276:immunology*; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000276:immunology; D022121:Cholera Vaccines / Q000008:administration & dosage / Q000276:immunology; D054307:Enterotoxigenic Escherichia coli / Q000276:immunology; D005260:Female; D006801:Humans; D007117:Immunization, Secondary; D007070:Immunoglobulin A / Q000097:blood / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007156:Immunologic Memory; D007700:Kinetics; D008297:Male; D014611:Vaccination / Q000379:methods; D055815:Young Adult
chemical_list: D001428:Bacterial Vaccines; D022121:Cholera Vaccines; C586175:Dukoral; D007070:Immunoglobulin A; D007074:Immunoglobulin G