title: Biodegradation of (1→3)-β-polyglucuronate prepared by TEMPO-mediated oxidation.
abstract: 2,2,6,6-Tetramethylpiperidine-1-oxyl radical (TEMPO)-mediated oxidation was applied to curdlan to prepare water-soluble sodium (1→3)-β-polyglucuronate, and its biodegradation behavior was then investigated. A bacterial strain (EH621) having the ability to degrade (1→3)-β-polyglucuronate was isolated from soil, and identified as Paenibacillus sp. Strain EH621 cultured with (1→3)-β-polyglucuronate decreased the initial total carbon in the supernatant by approximately 60% within 3 days, showing that (1→3)-β-polyglucuronate can be readily degraded and metabolized by microorganisms present in the natural environment. Hydrolytic enzyme activity was detected in the cell-free extract of EH621, which was highly specific to (1→3)-β-polyglucuronate. Analyses of the enzymatic degradation products revealed that (1→3)-β-polyglucuronate was endolytically degraded to its monomeric unit, glucuronate, via some oligomers and dimers.
authors.affiliation: Department of Applied Biological Chemistry, Utsunomiya University, Tochigi 321-8505, Japan.
authors.forename: Erika
authors.initials: E
authors.lastname: Watanabe
authors.forename: Naoto
authors.initials: N
authors.lastname: Habu
authors.forename: Akira
authors.initials: A
authors.lastname: Isogai
mesh_terms: D001483:Base Sequence; D001673:Biodegradation, Environmental; D003497:Cyclic N-Oxides / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D005965:Glucuronates / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D056507:Paenibacillus / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D047071:beta-Glucans / Q000737:chemistry
chemical_list: D003497:Cyclic N-Oxides; D004269:DNA, Bacterial; D005965:Glucuronates; D012336:RNA, Ribosomal, 16S; D047071:beta-Glucans; C038459:curdlan; C003959:TEMPO