title: IL-10 treatment is associated with prohibitin expression in the Crohn's disease intestinal fibrosis mouse model.
abstract: Prohibitin, which can inhibit oxidative stress and mitochondrial dysfunction, has been shown to have significant anti-inflammatory activities. Here, we investigate the effects of altering prohibitin levels in affected tissues in the interleukin-10 knockout (IL-10KO) mouse model with intestinal fibrosis. The aim of this study is to investigate the effects of IL-10 on prohibitin and the role of prohibitin in intestinal fibrosis of murine colitis. After the mice were treated with IL-10, prohibitin expression and localization were evaluated in IL-10KO and wild-type (WT, 129/SvEv) mice. The colon tissue was then investigated and the potential pathogenic molecular mechanisms were further studied. Fluorescence-based quantitative polymerase chain reaction (FQ-PCR) and immunohistochemistry assays revealed a significant upregulation of prohibitin with IL-10 treatment. Furthermore, IL-10 decreases inflammatory cytokines and TGF-β1 in the IL-10KO model of Crohn's disease and demonstrates a promising trend in decreasing tissue fibrosis. In conclusion, we hypothesize that IL-10 treatment is associated with increased prohibitin and would decrease inflammation and fibrosis in an animal model of Crohn's disease. Interestingly, prohibitin may be a potential target for intestinal fibrosis associated with inflammatory bowel disease (IBD).
authors.affiliation: Department of Gastroenterology, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University, Shanghai 200233, China.
authors.forename: C
authors.initials: C
authors.lastname: Yuan
authors.forename: W-X
authors.initials: WX
authors.lastname: Chen
authors.forename: J-S
authors.initials: JS
authors.lastname: Zhu
authors.forename: N-W
authors.initials: NW
authors.lastname: Chen
authors.forename: Y-M
authors.initials: YM
authors.lastname: Lu
authors.forename: Y-X
authors.initials: YX
authors.lastname: Ou
authors.forename: H-Q
authors.initials: HQ
authors.lastname: Chen
mesh_terms: D000818:Animals; D003092:Colitis / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism; D003106:Colon / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D003424:Crohn Disease / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005355:Fibrosis / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism*; D007150:Immunohistochemistry; D016753:Interleukin-10 / Q000172:deficiency / Q000235:genetics / Q000627:therapeutic use*; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000187:drug effects; D051379:Mice; D018345:Mice, Knockout; D000091402:Prohibitins; D060888:Real-Time Polymerase Chain Reaction; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000091402:Prohibitins; D012097:Repressor Proteins; D016753:Interleukin-10