title: Rapidly in situ forming chitosan/ε-polylysine hydrogels for adhesive sealants and hemostatic materials.
abstract: A novel in situ forming polysaccharides/polypeptide hydrogel composed of naturally derived materials for applications as adhesive sealant and hemostatic material was developed via Michael addition crosslinking, taking advantage of its mild condition. Thiol-modified chitosan (CSS) was fast in situ crosslinked by an efficient polypeptide crosslinker (EPLM) which was prepared by introducing maleimide groups onto ε-polylysine. Gelation can happen swiftly within 15-215s depending on the CSS concentration, the degree of substitution (DS) of maleimide groups, and the molar ratio of maleimide group to thiol group. Results indicated that storage modulus of the hydrogel increased dramatically with the increase of CSS concentration and DS of maleimide. The obtained adhesive hydrogel had an adhesion strength 4 times higher than that of the commercial fibrin glue. Notably, it is non-toxic to L929 cells and exhibits excellent prompt hemostatic property. Polysaccharides/polypeptide structure designed here facilitates to improve both the biocompatibility and the adhesive property.
authors.affiliation: School of Materials Science and Engineering, and Tianjin Key Laboratory of Composite and Functional Materials, Tianjin University, Tianjin 300072, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Nie
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Yuan
authors.forename: Jin
authors.initials: J
authors.lastname: Zhao
authors.forename: Yalin
authors.initials: Y
authors.lastname: Zhou
authors.forename: Huijing
authors.initials: H
authors.lastname: Bao
mesh_terms: D000268:Adhesiveness; D000269:Adhesives / Q000737:chemistry* / Q000494:pharmacology; D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology; D006490:Hemostatics / Q000737:chemistry* / Q000494:pharmacology; D020100:Hydrogels; D008099:Liver / Q000187:drug effects / Q000503:physiopathology; D008297:Male; D051379:Mice; D011107:Polylysine / Q000737:chemistry* / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012212:Rheology
chemical_list: D000269:Adhesives; D006490:Hemostatics; D020100:Hydrogels; D011107:Polylysine; D048271:Chitosan