title: Functional interrogation of kinases and other nucleotide-binding proteins.
abstract: The largest mammalian enzyme family is the kinases. Kinases and other nucleotide-binding proteins are key regulators of signal transduction pathways and the mutation or overexpression of these proteins is often the difference between health and disease. As a result, a massive research effort has focused on understanding how these proteins function and how to inhibit them for therapeutic benefit. Recent advances in chemical biological tools have enabled functional interrogation of these enzymes to provide a deeper understanding of their physiological roles. In addition, these innovative platforms have paved the way for a new generation of drugs whose properties have been guided by functional profiling.
authors.affiliation: ActivX Biosciences, Inc., 11025 N. Torrey Pines Rd, La Jolla, CA 92037, USA.
authors.forename: Jonathan S
authors.initials: JS
authors.lastname: Rosenblum
authors.forename: Tyzoon K
authors.initials: TK
authors.lastname: Nomanbhoy
authors.forename: John W
authors.initials: JW
authors.lastname: Kozarich
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D057075:Enzyme Assays; D006801:Humans; D010766:Phosphorylation; D010770:Phosphotransferases / Q000737:chemistry / Q000502:physiology*; D060066:Protein Interaction Maps; D011499:Protein Processing, Post-Translational; D040901:Proteomics
chemical_list: D000255:Adenosine Triphosphate; D010770:Phosphotransferases