title: [Expression pattern of hsa-miR-9 and its association with BCL6 in EBV-positive and EBV-negative Burkitt lymphoma cell lines].
abstract: OBJECTIVE
  To investigate the differential expression pattern of hsa-miR-9 between EBV-positive and -negative Burkitt lymphoma cell lines and its association with BCL-6.
  
  
  METHODS
  The expression of hsa-miR9 and BCL-6 mRNAs in EBV(+) Raji and EBV-Ramous cells in mRNA levels were detected using fluorescence quantitative PCR (QRT-PCR). The two cells lines were transiently transfected with hsa-mir9-inhibitor and hsa-mir9-minicsvia Oligofectamine 2000, and the changes in BCL6 expressions was detected using QRT-PCR and Western blotting. Annexin V/PI staining was used to analyze the apoptosis and morphological changes of the transfected cells.
  
  
  RESULTS
  The expression of Hsa-miR9 and BCL-6 was significantly higher in EBV(+) Raji cells than EBV(-) Ramous cells (P<0.01). BCL-6 mRNA and protein expression was reduced in EBV(+) Raji cells after transfection with hsa-miR9-inhibitor but up-regulated in EBV(-) Ramous cells transfected with hsa-miR9-minics. Flow cytometry revealed a significantly decreased apoptosis rate in EBV(+) Raji cells transfected with hsa-miR9-inhibitor but an increased rate in EBV(-) Ramous cells transfected with hsa-miR9-minics, and the results were confirmed by microscopic observations.
  
  
  CONCLUSIONS
  Hsa-miR9 positively regulate the expression of BCL-6 and apoptosis of EBV(+) Raji cells and EBV(-) Ramous cells.
authors.affiliation: Department of Pathology, Southern Medical University, Guangzhou, China. daixz2010@163.com
authors.forename: Xinzhen
authors.initials: X
authors.lastname: Dai
authors.forename: Shaohong
authors.initials: S
authors.lastname: Chen
authors.forename: Juan
authors.initials: J
authors.lastname: Ge
authors.forename: Xiqun
authors.initials: X
authors.lastname: Han
authors.forename: Xinhua
authors.initials: X
authors.lastname: Zhou
authors.forename: Ziqin
authors.initials: Z
authors.lastname: Wu
authors.forename: Tong
authors.initials: T
authors.lastname: Zhao
mesh_terms: D017209:Apoptosis; D002051:Burkitt Lymphoma / Q000235:genetics* / Q000473:pathology / Q000821:virology; D002455:Cell Division; D045744:Cell Line, Tumor; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic; D004854:Herpesvirus 4, Human; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D051560:Proto-Oncogene Proteins c-bcl-6; D014162:Transfection
chemical_list: C084103:BCL6 protein, human; D004268:DNA-Binding Proteins; C541874:MIRN92 microRNA, human; D035683:MicroRNAs; D051560:Proto-Oncogene Proteins c-bcl-6