title: Comparative evaluation of the Bruker Biotyper and Vitek MS matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry systems for identification of yeasts of medical importance.
abstract: We report the first comparative evaluation between the Bruker Biotyper MS (BMS) and the Vitek MS (VMS) for the identification of yeasts. The rate of correct identifications at the species level was comparable using the commercial databases (89.8% versus 84.3%; P = 0.712), but higher for BMS using an in-house-extended database (100% versus 84.3%; P = 0.245). Importantly, the rate of misidentification was significantly higher for VMS (1% versus 12.1%; P < 0.0001), including the rate of major errors (0% versus 4.5%; P = 0.0036).
authors.affiliation: Laboratorio di Microbiologia e Virologia, Università "Vita-Salute" San Raffaele, Milan, Italy. mancini.nicasio@hsr.it
authors.forename: Nicasio
authors.initials: N
authors.lastname: Mancini
authors.forename: Elena
authors.initials: E
authors.lastname: De Carolis
authors.forename: Laura
authors.initials: L
authors.lastname: Infurnari
authors.forename: Antonietta
authors.initials: A
authors.lastname: Vella
authors.forename: Nicola
authors.initials: N
authors.lastname: Clementi
authors.forename: Luisa
authors.initials: L
authors.lastname: Vaccaro
authors.forename: Alberto
authors.initials: A
authors.lastname: Ruggeri
authors.forename: Brunella
authors.initials: B
authors.lastname: Posteraro
authors.forename: Roberto
authors.initials: R
authors.lastname: Burioni
authors.forename: Massimo
authors.initials: M
authors.lastname: Clementi
authors.forename: Maurizio
authors.initials: M
authors.lastname: Sanguinetti
mesh_terms: D003951:Diagnostic Errors / Q000706:statistics & numerical data; D006801:Humans; D008828:Microbiological Techniques / Q000379:methods*; D016533:Mycological Typing Techniques / Q000379:methods*; D009172:Mycology / Q000379:methods*; D009181:Mycoses / Q000175:diagnosis* / Q000382:microbiology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013997:Time Factors; D015003:Yeasts / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification / Q000378:metabolism