title: Process optimization and production of polyhydroxybutyrate using palm jaggery as economical carbon source by marine sponge-associated Bacillus licheniformis MSBN12.
abstract: The Polyhydroxybutyrate (PHB) producer, Bacillus licheniformis MSBN12 was isolated from the marine sponge Callyspongia diffusa. The PHB production of B. licheniformis MSBN12 was optimized using a four-factor Box-Behnken design to find the interactive effects of variables such as palm jaggery, wheat bran, seawater, and incubation temperature. The maximum yield of PHB (6.38 g/L) was achieved through response surface methodology-based optimization and the optimized conditions were further used for the batch and fed-batch fermentation. Maximum biomass was reached at 48 and 36 h of incubation with PHB accumulation of 62.91 and 67.16 % (w/w of dry cells) for batch and fed-batch process. The production of PHB under fed-batch process with B. licheniformis MSBN12 was increased threefold over shake flask culture when palm jaggery as sole carbon source. The ¹H NMR data was extrapolated with peaks of the PHB reference standard and confirmed as PHB analog.
authors.affiliation: Department of Bioinformatics, Bharathidasan University, Tiruchirappalli, 620024, Tamil Nadu, India.
authors.forename: G
authors.initials: G
authors.lastname: Sathiyanarayanan
authors.forename: G
authors.initials: G
authors.lastname: Saibaba
authors.forename: G
authors.initials: G
authors.lastname: Seghal Kiran
authors.forename: Joseph
authors.initials: J
authors.lastname: Selvin
mesh_terms: D000818:Animals; D001407:Bacillus / Q000145:classification / Q000378:metabolism*; D001483:Base Sequence; D019149:Bioreactors; D002244:Carbon / Q000378:metabolism*; D017931:DNA Primers; D006863:Hydrogen-Ion Concentration; D006885:Hydroxybutyrates / Q000378:metabolism*; D008386:Marine Biology; D010802:Phylogeny; D011161:Porifera / Q000382:microbiology*; D013379:Substrate Specificity; D013696:Temperature
chemical_list: D017931:DNA Primers; D006885:Hydroxybutyrates; D002244:Carbon