title: Luminescent core-shell imprinted nanoparticles engineered for targeted Förster resonance energy transfer-based sensing.
abstract: Red-luminescent 200 nm silica nanoparticles have been designed and prepared as a versatile platform for developing FRET (Förster resonance energy transfer) biomimetic assays. Ru(phen)₃²⁺ dye molecules embedded off-center in the silica core provide the long-lived donor emission, and a near-infrared labeled analyte serves as fluorescent acceptor (the measured R₀ of this D-A pair is 4.3 nm). A thin surface-grafted molecularly imprinted polymer (MIP) shell intervenes as selective enrofloxacin-binding element. These nanoparticles have been tested for photochemical detection of enrofloxacin by using a competitive scheme that can be readily performed in MeCN-HEPES (pH 7.5) 7:3 (v/v) mixtures and allows for the antibiotic detection in the μM range (LOD = 2 μM) without optimization of the assay. Given the well-known difficulties of coupling the target-binding-to-MIP and the transducing events, the novel photochemical approach tuned up here will be valuable in future developments of MIP-based assays and optosensors that capitalize also on the advantages of nanomaterials for (bio)analysis.
authors.forename: Ana B
authors.initials: AB
authors.lastname: Descalzo
authors.forename: Clara
authors.initials: C
authors.lastname: Somoza
authors.forename: María C
authors.initials: MC
authors.lastname: Moreno-Bondi
authors.forename: Guillermo
authors.initials: G
authors.lastname: Orellana
mesh_terms: D000970:Antineoplastic Agents / Q000032:analysis; D015374:Biosensing Techniques / Q000379:methods*; D000077422:Enrofloxacin; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D024841:Fluoroquinolones / Q000032:analysis*; D049449:Luminescence; D053768:Metal Nanoparticles / Q000737:chemistry*; D054802:Molecular Imprinting / Q000379:methods*; D055668:Photochemical Processes; D011108:Polymers / Q000737:chemistry*; D012428:Ruthenium / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: D000970:Antineoplastic Agents; D024841:Fluoroquinolones; D011108:Polymers; D000077422:Enrofloxacin; D012822:Silicon Dioxide; D012428:Ruthenium