title: Method development and validation of olprinone in human plasma by HPLC coupled with ESI-MS-MS: application to a pharmacokinetic study.
abstract: A simple, specific and highly sensitive assay using liquid chromatography-tandem mass spectrometry with electrospray ionization was developed for the determination of olprinone in human plasma. Following the addition of codeine as an internal standard and simple liquid-liquid extraction with ethyl acetate-isopropanol (95:5, v/v), the analytes were separated on a Zorbax SB-C18 column (4.6 × 150 mm i.d., 5 µm) and eluted with a mobile phase consisting of methanol-10 mmol/L ammonium acetate containing 1% formic acid (50:50, v/v) at a flow rate of 1.0 mL/min. Detection was achieved with electrospray positive ionization mass spectrometry using multiple reaction monitoring mode. Linear calibration curves were obtained in the concentration range of 0.1-100 ng/mL (r = 0.9995), with a lower limit of quantification of 0.1 ng/mL. The intra-day and inter-day precision values, as relative deviation, were below 6.06% and the accuracy, as relative error, was below 9.92% at all quality control concentrations. The method was applicable to clinical pharmacokinetic study of an olprinone hydrochloride hydrate injection in healthy Chinese volunteers.
authors.affiliation: 1School of Pharmaceutical Sciences, Jilin University, Changchun 130021, P.R. China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Wang
authors.forename: Baochang
authors.initials: B
authors.lastname: Zhang
authors.forename: Xilin
authors.initials: X
authors.lastname: Sun
authors.forename: Yantong
authors.initials: Y
authors.lastname: Sun
authors.forename: Meiyun
authors.initials: M
authors.lastname: Shi
mesh_terms: D000293:Adolescent; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006207:Half-Life; D006801:Humans; D007093:Imidazoles / Q000302:isolation & purification* / Q000493:pharmacokinetics; D008297:Male; D058987:Phosphodiesterase 3 Inhibitors / Q000302:isolation & purification* / Q000493:pharmacokinetics; D011728:Pyridones / Q000302:isolation & purification* / Q000493:pharmacokinetics; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D055815:Young Adult
chemical_list: D007093:Imidazoles; D058987:Phosphodiesterase 3 Inhibitors; D011728:Pyridones; C059498:olprinone