title: Effect of turmeric and carrot seed extracts on serum liver biomarkers and hepatic lipid peroxidation, antioxidant enzymes and total antioxidant status in rats.
abstract: BACKGROUND
  Pathogenic role of free radicals are well known in various metabolic diseases. They originate from internal and external sources of body. Essential roles of antioxidant defense system for cellular redox regulation and free radical scavenging activity were described in this study. Many in vitro investigations have shown that turmeric (TE) and carrot seed extract (CSE) exhibits to possess antioxidant activities. In this study, we evaluated the antioxidant potentials of ethanolic TE and CSE based on in vivo experiment in the rats.
  
  
  METHODS
  ANIMALS WERE ASSIGNED TO SIX GROUPS: the 1st and 2nd groups were control groups and 2nd group received 0.2 ml dimethyl sulphoxide as vehicle treated group; other four experimental groups received different doses of TE (100, 200 mg/kg b.w.) and CSE (200, 400 mg/kg b.w.) by gavages, respectively for a period of one month. The indicators of oxidative stress, lipids peroxidation, markers of hepatocyte injury and biliary function markers were measured.
  
  
  RESULTS
  The levels of superoxide dismutase, catalase, and glutathione peroxidase were significantly stimulated in the hepatic tissue of treatment groups. The malondialdehyde contents of liver tissue were significantly reduced in the groups fed with TE and CSE. Serum levels of alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase, in treated groups were found to be significantly decreased, whereas albumin and total protein increased as compared to the control groups (P<0.05).
  
  
  CONCLUSIONS
  this study showed that the regular intake of TE and CSE through the diet can improve antioxidant status and inhibit peroxidation activity in the liver tissue so that using these extracts may protect tissue oxidative stress.
authors.affiliation: Young Researchers Club, Ardabil Branch, Islamic Azad University, Ardabil, Iran.
authors.forename: Adel
authors.initials: A
authors.lastname: Rezaei-Moghadam
authors.forename: Daryoush
authors.initials: D
authors.lastname: Mohajeri
authors.forename: Behnam
authors.initials: B
authors.lastname: Rafiei
authors.forename: Rana
authors.initials: R
authors.lastname: Dizaji
authors.forename: Asghar
authors.initials: A
authors.lastname: Azhdari
authors.forename: Mahdi
authors.initials: M
authors.lastname: Yeganehzad
authors.forename: Maryamossadat
authors.initials: M
authors.lastname: Shahidi
authors.forename: Mohammad
authors.initials: M
authors.lastname: Mazani
keywords: Antioxidant Enzymes; Carrot Seed Extract; Lipid Peroxidation; Serum Liver Biomarkers; Total Antioxidant Status; Turmeric Extract