title: Effect of hypericin on the ADAMTS-9 and ADAMTS-8 gene expression in MCF7 breast cancer cells.
abstract: OBJECTIVE
  To investigate the effects of hypericin which is obtained from the plant Hypericum perforatum on the expression and the regulation of ADAMTS8 and ADAMTS9 genes in MCF7 breast cancer cells and on the viability of these cells.
  
  
  METHODS
  MCF7 cells were cultured and were separately exposed to 2, 10 and 50 µl/mL of hypericin. After 24 hours, RNA was isolated from these cells and converted to cDNA. The expression levels of ADAMTS8 and ADAMTS9 genes were evaluated using the Reverse Transcription Polymerase Chain Reaction. XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide, disodium salt) cell viability assay was used to determine cytotoxicity.
  
  
  RESULTS
  ADAMTS9 expression in MCF7 cells were increased 1.8 and 3.6 fold with the use of 2 and 10 µl/mL of hypericin, respectively; and decreased 0.7 fold with the use of 50 µl/mL of hypericin. There was no significant change in the ADAMTS8 expression. Rapid cell death was observed in the cancer cells when hypericin was used at a dose of ≥ 50 µl/mL.
  
  
  CONCLUSIONS
  The increase in ADAMTS9 expression can be a useful factor in the prevention of possible metastasis in breast cancer and for the occurrence of a tumor suppressive effect. Hypericin increases the expression of ADAMTS9, therefore, it may show its antitumoral and antiapoptotic effects by means of ADAMTS9.
authors.affiliation: Department of Medical Genetics, Abant Izzet Baysal University, Bolu, Turkey. zeynep_ipek@yahoo.com
authors.forename: Z
authors.initials: Z
authors.lastname: Ocak
authors.forename: M
authors.initials: M
authors.lastname: Acar
authors.forename: E
authors.initials: E
authors.lastname: Gunduz
authors.forename: M
authors.initials: M
authors.lastname: Gunduz
authors.forename: K
authors.initials: K
authors.lastname: Demircan
authors.forename: U
authors.initials: U
authors.lastname: Uyeturk
authors.forename: T
authors.initials: T
authors.lastname: Ozlü
mesh_terms: D051722:ADAM Proteins / Q000235:genetics*; D000071096:ADAMTS Proteins; D000071119:ADAMTS9 Protein; D000873:Anthracenes; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004396:Coloring Agents; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D020902:Hypericum / Q000737:chemistry; D010569:Perylene / Q000031:analogs & derivatives* / Q000494:pharmacology; D012334:RNA, Neoplasm / Q000096:biosynthesis / Q000302:isolation & purification; D060888:Real-Time Polymerase Chain Reaction; D013778:Tetrazolium Salts
chemical_list: D000873:Anthracenes; D004396:Coloring Agents; D012334:RNA, Neoplasm; D013778:Tetrazolium Salts; C059087:2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide; D010569:Perylene; C004965:hypericin; D051722:ADAM Proteins; D000071096:ADAMTS Proteins; C120807:ADAMTS8 protein, human; D000071119:ADAMTS9 Protein; C412869:ADAMTS9 protein, human