title: Protein-protein interaction analysis in single microfluidic droplets using FRET and fluorescence lifetime detection.
abstract: Herein, we demonstrate the feasibility of a protein-protein interaction analysis and reaction progress monitoring in microfluidic droplets using FRET and microscopic fluorescence lifetime measurements. The fabrication of microdroplet chips using soft- and photolithographic techniques is demonstrated and the resulting chips reliably generate microdroplets of 630 pL and 6.71 nL at frequencies of 7.9 and 0.75 Hz, respectively. They were used for detection of protein-protein interactions in microdroplets using a model system of Alexa Fluor 488 labelled biotinylated BSA, Alexa Fluor 594 labelled streptavidin and unlabelled chicken egg white avidin. These microchips could be used for quantitative detection of avidin and streptavidin in microdroplets in direct and competitive assay formats with nanomolar detection limits, corresponding to attomole protein amounts. Four droplets were found to be sufficient for analytical determination. Fluorescence intensity ratio and fluorescence lifetime measurements were performed and compared for microdroplet FRET determination. A competitive on-chip binding assay for determination of unlabelled avidin using fluorescence lifetime detection could be performed within 135 s only.
authors.affiliation: Institut für Analytische Chemie, Universität Leipzig, Johannisallee 29, Leipzig, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Benz
authors.forename: Heiko
authors.initials: H
authors.lastname: Retzbach
authors.forename: Stefan
authors.initials: S
authors.lastname: Nagl
authors.forename: Detlev
authors.initials: D
authors.lastname: Belder
mesh_terms: D001360:Avidin / Q000737:chemistry; D001710:Biotin / Q000737:chemistry; D004867:Equipment Design; D031541:Fluorescence Resonance Energy Transfer; D005470:Fluorometry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D011485:Protein Binding; D025941:Protein Interaction Mapping / Q000295:instrumentation / Q000379:methods*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D019809:Streptavidin / Q000737:chemistry; D013997:Time Factors
chemical_list: D001360:Avidin; D012710:Serum Albumin, Bovine; D001710:Biotin; D019809:Streptavidin