title: [Development and evaluation of colloidal gold immunochromatography assay (GICA) for detection of antibody against Schistosoma japonica in smaller serum].
abstract: OBJECTIVE
  To develop a rapid colloidal gold immunochromatography assay (GICA) for detection of the antibody to schistosome in 5 microl sera.
  
  
  METHODS
  A soluble egg antigen (SEA) from Schistosomajaponica was separated with sephacryl S-300 column to retain the active antigen fractions to the antibody of schistosome. The optional reaction system and detection kit with 5 microl sera were established by improving conjugated concentration and formulas of sample buffer and labeled solution.
  
  
  RESULTS
  The sensitivity of detecting schistosomiasis patients whose stool examinations for schistosome eggs were positive was 93.7%, the specificity to health population 97.1%, the cross reaction rate to patients with paragonimiasis 5.6%. The Youden' s index value was 0.91. There was 96.1% crude coincidence of GICA and ELISA in detecting 507 cases of floating population and the Kappa value was 0.81.
  
  
  CONCLUSIONS
  GICA kit is practical for detection of schistosomiasis in the field because of its advantages such as smaller serum needed, the high sensitivity, lower cross reaction rate and spread application for human and animals.
authors.affiliation: Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, China.
authors.forename: Shou-Fu
authors.initials: SF
authors.lastname: Jiang
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Zhang
authors.forename: Bao-Liang
authors.initials: BL
authors.lastname: Li
authors.forename: Yan-Yan
authors.initials: YY
authors.lastname: He
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Yue-Hong
authors.initials: YH
authors.lastname: Tang
authors.forename: Xiao-Jiang
authors.initials: XJ
authors.lastname: Ma
authors.forename: Li
authors.initials: L
authors.lastname: Cai
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood* / Q000276:immunology*; D000947:Antigens, Helminth / Q000276:immunology / Q000302:isolation & purification; D002846:Chromatography, Affinity / Q000379:methods*; D017979:Gold Colloid / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D015203:Reproducibility of Results; D012549:Schistosoma japonicum / Q000276:immunology*
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D017979:Gold Colloid