title: β-Lactoglobulin-linoleate complexes: In vitro digestion and the role of protein in fatty acid uptake.
abstract: The dairy protein β-lactoglobulin (BLG) is known to bind fatty acids such as the salt of the essential longchain fatty acid linoleic acid (cis,cis-9,12-octadecadienoic acid, n-6, 18:2). The aim of the current study was to investigate how bovine BLG-linoleate complexes, of various stoichiometry, affect the enzymatic digestion of BLG and the intracellular transport of linoleate into enterocyte-like monolayers. Duodenal and gastric digestions of the complexes indicated that BLG was hydrolyzed more rapidly when complexed with linoleate. Digested as well as undigested BLG-linoleate complexes reduced intracellular linoleate transport as compared with free linoleate. To investigate whether enteroendocrine cells perceive linoleate differently when part of a complex, the ability of linoleate to increase production or secretion of the enteroendocrine satiety hormone, cholecystokinin, was measured. Cholecystokinin mRNA levels were different when linoleate was presented to the cells alone or as part of a protein complex. In conclusion, understanding interactions between linoleate and BLG could help to formulate foods with targeted fatty acid bioaccessibility and, therefore, aid in the development of food matrices with optimal bioactive efficacy.
authors.affiliation: INRA, UMR1253 STLO, 65 rue de Saint Brieuc, F-35042 Rennes, France.
authors.forename: Solène
authors.initials: S
authors.lastname: Le Maux
authors.forename: André
authors.initials: A
authors.lastname: Brodkorb
authors.forename: Thomas
authors.initials: T
authors.lastname: Croguennec
authors.forename: Alan A
authors.initials: AA
authors.lastname: Hennessy
authors.forename: Saïd
authors.initials: S
authors.lastname: Bouhallab
authors.forename: Linda
authors.initials: L
authors.lastname: Giblin
mesh_terms: D000818:Animals; D001692:Biological Transport; D018938:Caco-2 Cells / Q000378:metabolism; D002417:Cattle; D002766:Cholecystokinin / Q000235:genetics / Q000378:metabolism; D004063:Digestion; D004847:Epithelial Cells / Q000378:metabolism; D005227:Fatty Acids / Q000493:pharmacokinetics*; D006801:Humans; D066298:In Vitro Techniques; D007782:Lactoglobulins / Q000502:physiology*; D019787:Linoleic Acid / Q000378:metabolism / Q000493:pharmacokinetics*; D008892:Milk / Q000737:chemistry*; D012333:RNA, Messenger / Q000032:analysis
chemical_list: D005227:Fatty Acids; D007782:Lactoglobulins; D012333:RNA, Messenger; D002766:Cholecystokinin; D019787:Linoleic Acid