title: Application of mimotope peptides of fumonisin b1 in Peptide ELISA.
abstract: Anti-fumonisin B(1) (FB(1)) McAb 1D11 was used as the target for biopanning from a phage random loop-constrained heptapeptide library. After three cycles of panning, seven phages with three mimotope peptides were selected to mimic the binding of FB(1) to 1D11. After the identification of phage ELISA, the phage clone that showed the best linear range of detection was chosen for further research. One peptide with the inserted peptide sequence of the phage was synthetized, named CT-452. An indirect competitive ELISA (peptide ELISA) for detecting FB(1) was established using the CT-452-bovine serum albumin conjugate as coating antigen. The linear range of the inhibition curve was 1.77-20.73 ng/mL. The half inhibitory concentration (IC50) was 6.06 ng/mL, and the limit of detection was 1.18 ng/mL. This method was compared with conventional indirect ELISA (commercial ELISA kit) and high-performance liquid chromatography (HPLC), and the results showed the reliability of the peptide ELISA for the determination of FB(1) in cereal samples. The relationship between the CT-452 and FB(1) standard concentrations in peptide ELISA was evaluated. The results indicated that synthetic peptide CT-452 can replace the FB(1) standard to establish an immunoassay free of FB(1).
authors.affiliation: State Key Laboratory of Food Science and Technology, Sino-Germany Joint Research Institute, Nanchang University, No. 235 Nanjing East Road, Nanchang 330047, China.
authors.forename: Xing
authors.initials: X
authors.lastname: Liu
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
authors.forename: Qing-hua
authors.initials: QH
authors.lastname: He
authors.forename: Zhen-yun
authors.initials: ZY
authors.lastname: He
authors.forename: Zheng-ping
authors.initials: ZP
authors.lastname: Xiong
mesh_terms: D000911:Antibodies, Monoclonal; D002851:Chromatography, High Pressure Liquid; D002523:Edible Grain / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D037341:Fumonisins / Q000032:analysis* / Q000737:chemistry* / Q000276:immunology; D018716:Molecular Mimicry; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry / Q000276:immunology*; D015203:Reproducibility of Results
chemical_list: D000911:Antibodies, Monoclonal; D037341:Fumonisins; D019151:Peptide Library; D010455:Peptides; C056933:fumonisin B1