title: miR-146a and miR-150 promote the differentiation of CD133+ cells into T-lymphoid lineage.
abstract: MicroRNAs control the genes involved in hematopoietic stem cell (HSCs) survival, proliferation and differentiation. The over-expression of miR-146 and miR-150 has been reported during differentiation of HSCs into T-lymphoid lineage. Therefore, in this study we evaluated the effect of their over-expression on CD133+ cells differentiation to T cells. miR-146a and miR-150 were separately and jointly transduced to human cord blood derived CD133+ cells (>97% purity). We used qRT-PCR to assess the expression of CD2, CD3ε, CD4, CD8, CD25, T cell receptor alpha (TCR-α) and Ikaros genes in differentiated cells 4 and 8 days after transduction of the miRNAs. Following the over-expression of miR-146a, significant up-regulation of CD2, CD4, CD25 and Ikaros genes were observed (P<0.01). On the other hand, over-expression of miR-150 caused an increase in the expression of Ikaros, CD4, CD25 and TCR-α. To evaluate the combinatorial effect of miR-146a and miR-150, transduction of both miRNAs was concurrently performed which led to increase in the expression of Ikaros, CD4 and CD3 genes. In conclusion, it seems that the effect of miR-150 and miR-146a on the promotion of T cell differentiation is time-dependant. Moreover, miRNAs could be used either as substitutes or complements of the conventional differentiation protocols for higher efficiency.
authors.affiliation: Department of Molecular Biology and Genetic Engineering, Stem Cell Technology Research Center, 1997775555, Tehran, Iran.
authors.forename: Parviz
authors.initials: P
authors.lastname: Fallah
authors.forename: Ehsan
authors.initials: E
authors.lastname: Arefian
authors.forename: Mahmood
authors.initials: M
authors.lastname: Naderi
authors.forename: Seyed Hamid
authors.initials: SH
authors.lastname: Aghaee-Bakhtiari
authors.forename: Amir
authors.initials: A
authors.lastname: Atashi
authors.forename: Katayoun
authors.initials: K
authors.lastname: Ahmadi
authors.forename: Abbas
authors.initials: A
authors.lastname: Shafiee
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
mesh_terms: D000071916:AC133 Antigen; D000704:Analysis of Variance; D015703:Antigens, CD / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D017931:DNA Primers / Q000235:genetics; D005312:Fetal Blood / Q000166:cytology; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000276:immunology*; D005822:Genetic Vectors; D006023:Glycoproteins / Q000378:metabolism*; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D013601:T-Lymphocytes / Q000166:cytology*
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D017931:DNA Primers; D006023:Glycoproteins; D035683:MicroRNAs; D010455:Peptides; C542542:MIRN146 microRNA, human; C533097:MIR150, human; C000606023:PROM1 protein, human