title: Development and characterization of multilamellar liposomes containing pyridostigmine.
abstract: Pyridostigmine has cardioprotective activity in both free and liposomal forms. This study aimed to develop and characterize liposomal formulations of pyridostigmine. For this, a spectrophotometric ultraviolet (UV) analytical method, at 270 nm, was developed and validated to quantify liposomal pyridostigmine. The method was linear in ranges from 0.02 to 0.09 mg/mL. The accuracy of this method was determined intra- and inter-day; the results of coefficient of variation were of 1.73-2.72% and 0.32-2.32%, respectively. The accuracy ranged between 99.45% and 101.12%. The method has not changed by influence of liposomal matrix and demonstrated being able to quantify pyridostigmine in liposomes. Two liposomal multilamellar formulations were developed: a constituted by dystearoyl-phosphatidylcholine (DSPC) and cholesterol (CHOL) other by dioleil-phosphatidylcholine (DOPC) and CHOL. The encapsulation efficiency was determined as 23.4% and 15.4%, respectively. Analyses of size and release of pyridostigmine from the formulations were made and the results showed that the formulations are viable for future studies in vivo.
authors.affiliation: CiPharma, Escola de Farmácia, Universidade Federal de Ouro Preto , Ouro Preto, Minas Gerais , Brasil and.
authors.forename: Ana Carolina Moreira
authors.initials: AC
authors.lastname: Souza
authors.forename: Andrea
authors.initials: A
authors.lastname: Grabe-Guimarães
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Souza
authors.forename: Wallace Entringer
authors.initials: WE
authors.lastname: Botacim
authors.forename: Tamara Marine
authors.initials: TM
authors.lastname: Almeida
authors.forename: Fréderic Jean Georges
authors.initials: FJ
authors.lastname: Frézard
authors.forename: Neila Márcia
authors.initials: NM
authors.lastname: Silva Barcellos
mesh_terms: D002626:Chemistry, Pharmaceutical / Q000379:methods; D002784:Cholesterol / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D010316:Particle Size; D010713:Phosphatidylcholines / Q000737:chemistry; D011729:Pyridostigmine Bromide / Q000737:chemistry*
chemical_list: D004337:Drug Carriers; D008081:Liposomes; D010713:Phosphatidylcholines; D002784:Cholesterol; C017251:1,2-oleoylphosphatidylcholine; D011729:Pyridostigmine Bromide