title: Introduction to mass spectrometry-based proteomics.
abstract: Mass spectrometry has been widely applied to study biomolecules and one rapidly developing field is the global analysis of proteins, proteomics. Understanding and handling mass spectrometry data is a multifaceted task that requires many decisions to be made to get the most comprehensive information from an experiment. Later chapters in this book deal in-depth with various aspects of the process and how different tools can be applied to the many analytical challenges. This introductory chapter is intended as a basic introduction to mass spectrometry (MS)-based proteomics to set the scene for newcomers and give pointers to reference material. There are many applications of mass spectrometry in proteomics and each application is associated with some analytical choices, instrumental limitations and data processing steps that depend on the aim of the study and means of conducting it. Different aspects of the proteome can be explored by choosing the right combination of sample preparation, MS instrumentation and data processing. This chapter gives an outline for some of these commonly used setups and some of the key concepts, many of which are explored in greater depth in later chapters.
authors.affiliation: Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP), Porto, Portugal.
authors.forename: Rune
authors.initials: R
authors.lastname: Matthiesen
authors.forename: Jakob
authors.initials: J
authors.lastname: Bunkenborg
mesh_terms: D013058:Mass Spectrometry / Q000295:instrumentation* / Q000379:methods*; D011506:Proteins / Q000032:analysis; D020543:Proteome / Q000737:chemistry; D040901:Proteomics / Q000379:methods*
chemical_list: D011506:Proteins; D020543:Proteome