title: The putrescine biosynthesis pathway in Lactococcus lactis is transcriptionally regulated by carbon catabolic repression, mediated by CcpA.
abstract: Lactococcus lactis is the lactic acid bacterium most widely used by the dairy industry as a starter for the manufacture of fermented products such as cheese and buttermilk. However, some strains produce putrescine from agmatine via the agmatine deiminase (AGDI) pathway. The proteins involved in this pathway, including those necessary for agmatine uptake and conversion into putrescine, are encoded by the aguB, aguD, aguA and aguC genes, which together form an operon. This paper reports the mechanism of regulation of putrescine biosynthesis in L. lactis. It is shown that the aguBDAC operon, which contains a cre site at the promoter of aguB (the first gene of the operon), is transcriptionally regulated by carbon catabolic repression (CCR) mediated by the catabolite control protein CcpA.
authors.affiliation: Instituto de Productos Lácteos de Asturias (IPLA-CSIC), Paseo Río Linares s/n, 33300 Villaviciosa, Asturias, Spain.
authors.forename: Daniel M
authors.initials: DM
authors.lastname: Linares
authors.forename: Beatriz
authors.initials: B
authors.lastname: del Río
authors.forename: Victor
authors.initials: V
authors.lastname: Ladero
authors.forename: Begoña
authors.initials: B
authors.lastname: Redruello
authors.forename: María Cruz
authors.initials: MC
authors.lastname: Martín
authors.forename: María
authors.initials: M
authors.lastname: Fernández
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Alvarez
mesh_terms: D002244:Carbon / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005798:Genes, Bacterial / Q000235:genetics; D005947:Glucose / Q000378:metabolism / Q000494:pharmacology; D013294:Lactococcus lactis / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D005810:Multigene Family / Q000235:genetics; D011700:Putrescine / Q000096:biosynthesis*
chemical_list: D002244:Carbon; D005947:Glucose; D011700:Putrescine