title: A method of experimental rheumatoid arthritis induction using collagen type II isolated from chicken sternal cartilage.
abstract: At present, collagen‑induced arthritis (CIA) is the best known and most extensively used model for the immunological and pathological characteristics of human rheumatoid arthritis (RA). This model is useful not only in aiding our understanding of the pathogenesis of this disease, but also in the development of new therapies. Bovine, porcine and human collagen has been used to induce CIA; however, response has been identified to vary between strains and injection conditions, and false positive results and reduced potency are common as a result of minor contaminants or deglycosylated protein. Therefore, in the present study, type II collagen (CII) was isolated and purified from chicken sternal cartilage and was found to successfully induce the RA model. Furthermore, T helper 17 (Th17) cells were observed to infiltrate the joint on day 45 following induction by CII. In vitro, expression of toll‑like receptor 2 (TLR2) increased in peritoneal macrophages stimulated by CII. In addition, blockage of TLR2 was identified to markedly decrease levels of TGF‑β and IL‑6 in the cell culture supernatant. The results indicate that CII isolated from chicken sternal cartilage may be recognized by TLR2 on macrophages, leading to TGF‑β and IL‑6 production and subsequent activation of Th17 cells which mediates CIA development.
authors.affiliation: Department of Immunology and Laboratory Immunology, Jiangsu University, Zhenjiang 212013, PR China. szl30@yeah.net
authors.forename: Zhaoliang
authors.initials: Z
authors.lastname: Su
authors.forename: Siamak Sandoghchian
authors.initials: SS
authors.lastname: Shotorbani
authors.forename: Xugan
authors.initials: X
authors.lastname: Jiang
authors.forename: Rui
authors.initials: R
authors.lastname: Ma
authors.forename: Huiling
authors.initials: H
authors.lastname: Shen
authors.forename: Fanzhi
authors.initials: F
authors.lastname: Kong
authors.forename: Huaxi
authors.initials: H
authors.lastname: Xu
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000276:immunology* / Q000378:metabolism; D001172:Arthritis, Rheumatoid / Q000276:immunology* / Q000378:metabolism; D002356:Cartilage / Q000737:chemistry; D002645:Chickens; D024043:Collagen Type II / Q000276:immunology* / Q000302:isolation & purification; D015850:Interleukin-6 / Q000096:biosynthesis; D017737:Macrophages, Peritoneal / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D015398:Signal Transduction; D058504:Th17 Cells / Q000276:immunology / Q000378:metabolism; D051195:Toll-Like Receptor 2 / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000096:biosynthesis
chemical_list: D024043:Collagen Type II; D015850:Interleukin-6; D051195:Toll-Like Receptor 2; D016212:Transforming Growth Factor beta