title: Effects of polysaccharides from different species of Dendrobium (Shihu) on macrophage function.
abstract: Dendrobium spp. are precious medicinal plants, used in China for thousands of years as health foods and nutrients. Polysaccharides are the main effective ingredients in Dendrobium plants. In this study, the chemical characteristics and the effects of crude polysaccharides (CPs) from five species of Dendrobium on macrophage function were investigated and compared in vitro for the first time. Chemical characteristic studies showed that CPs from different species of Dendrobium were diverse, displaying widely varied Mw distributions and molar ratios of monosaccharides. Their effects on macrophage functions, such as promoting phagocytosis, release of NO and cytokines IL-1α, IL-6, IL-10 and TNF-α, were also different. Moreover, CPs from D. officinale, especially collected from Yunnan Province, exerted the strongest immunomodulatory activities and could be explored as a novel potential functional food. The diverse chemical characteristics of CPs from different species of Dendrobium might contribute to their varied effects on macrophage functions, which should be further investigated.
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China.
authors.forename: Lan-Zhen
authors.initials: LZ
authors.lastname: Meng
authors.forename: Guang-Ping
authors.initials: GP
authors.lastname: Lv
authors.forename: De-Jun
authors.initials: DJ
authors.lastname: Hu
authors.forename: Kit-Leong
authors.initials: KL
authors.lastname: Cheong
authors.forename: Jing
authors.initials: J
authors.lastname: Xie
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Shao-Ping
authors.initials: SP
authors.lastname: Li
mesh_terms: D000818:Animals; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis*; D031667:Dendrobium / Q000737:chemistry*; D007155:Immunologic Factors / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects*; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D016207:Cytokines; D007155:Immunologic Factors; D011134:Polysaccharides