title: A protein phosphatase 2A complex spatially controls plant cell division.
abstract: In the absence of cell migration, the orientation of cell divisions is crucial for body plan determination in plants. The position of the division plane in plant cells is set up premitotically via a transient cytoskeletal array, the preprophase band, which precisely delineates the cortical plane of division. Here we describe a protein complex that targets protein phosphatase 2A activity to microtubules, regulating the transition from the interphase to the premitotic microtubule array. This complex, which comprises TONNEAU1 and a PP2A heterotrimeric holoenzyme with FASS as regulatory subunit, is recruited to the cytoskeleton via the TONNEAU1-recruiting motif family of proteins. Despite the acentrosomal nature of plant cells, all members of this complex share similarity with animal centrosomal proteins involved in ciliary and centriolar/centrosomal functions, revealing an evolutionary link between the cortical cytoskeleton of plant cells and microtubule organizers in other eukaryotes.
authors.affiliation: INRA, Institut Jean-Pierre Bourgin UMR1318, Saclay Plant Science, 78000 Versailles, France.
authors.forename: Lara
authors.initials: L
authors.lastname: Spinner
authors.forename: Astrid
authors.initials: A
authors.lastname: Gadeyne
authors.forename: Katia
authors.initials: K
authors.lastname: Belcram
authors.forename: Magali
authors.initials: M
authors.lastname: Goussot
authors.forename: Michaël
authors.initials: M
authors.lastname: Moison
authors.forename: Yann
authors.initials: Y
authors.lastname: Duroc
authors.forename: Dominique
authors.initials: D
authors.lastname: Eeckhout
authors.forename: Nancy
authors.initials: N
authors.lastname: De Winne
authors.forename: Estelle
authors.initials: E
authors.lastname: Schaefer
authors.forename: Eveline
authors.initials: E
authors.lastname: Van De Slijke
authors.forename: Geert
authors.initials: G
authors.lastname: Persiau
authors.forename: Erwin
authors.initials: E
authors.lastname: Witters
authors.forename: Kris
authors.initials: K
authors.lastname: Gevaert
authors.forename: Geert
authors.initials: G
authors.lastname: De Jaeger
authors.forename: David
authors.initials: D
authors.lastname: Bouchez
authors.forename: Daniël
authors.initials: D
authors.lastname: Van Damme
authors.forename: Martine
authors.initials: M
authors.lastname: Pastuglia
mesh_terms: D000483:Alleles; D017360:Arabidopsis / Q000166:cytology* / Q000201:enzymology* / Q000648:ultrastructure; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002455:Cell Division; D018525:Germination; D007527:Isoenzymes / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D008870:Microtubules / Q000378:metabolism; D046912:Multiprotein Complexes / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D010641:Phenotype; D010749:Phosphoprotein Phosphatases / Q000378:metabolism; D059828:Plant Cells / Q000201:enzymology*; D011418:Prophase; D011485:Protein Binding; D060066:Protein Interaction Maps; D054648:Protein Phosphatase 2 / Q000235:genetics / Q000378:metabolism*; D036226:Seedlings / Q000648:ultrastructure
chemical_list: D029681:Arabidopsis Proteins; C460109:FASS protein, Arabidopsis; D007527:Isoenzymes; D008869:Microtubule-Associated Proteins; D046912:Multiprotein Complexes; C585274:tonneau1 protein, Arabidopsis; D010749:Phosphoprotein Phosphatases; D054648:Protein Phosphatase 2