title: Effect of basic fibroblast growth factor on the myocardial expression of hypoxia-inducible factor-1α and vascular endothelial growth factor following acute myocardial infarction.
abstract: OBJECTIVE
  To investigate the effect of basic fibroblast growth factor (bFGF) on myocardial expression of hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) following acute myocardial infarction (AMI) in rats.
  
  
  METHODS
  Eighty male Sprague-Dawley rats were divided into sham operation (n=20), AMI control (n=20), bFGF50 (intravenous bFGF 50 μg/kg/d, n=20) and bFGF200 (intravenous bFGF 200 μg/kg/d, n=20) groups. The left ventricular ejection fraction (LVEF) was measured by echocardiography. The expression of HIF-1α mRNA and VEGF mRNA in the ischaemic tissues was analysed by reverse transcription-polymerase chain reaction.
  
  
  RESULTS
  The LVEF in the bFGF50 and bFGF200 group was higher than in the AMI control group (p<0.05) seven and 14 days after the treatment. There was no difference in HIF-1α mRNA expression between the bFGF50 and AMI control group (p>0.05). However, the HIF-1α mRNA expression in the bFGF200 group was higher than in the AMI control group seven days (1.13 ± 0.18 vs 0.90 ± 0.14, p<0.01) and 14 days (1.31 ± 0.18 vs 0.93 ± 0.09, p<0.01) after the treatment. The VEGF mRNA expression in the bFGF200 group was also higher than in the AMI control group seven days (1.10 ± 0.17 vs 0.86 ± 0.14, p<0.01) and 14 days after the AMI (1.28 ± 0.19 vs 0.89 ± 0.14, p<0.01).
  
  
  CONCLUSIONS
  bFGF therapy was associated with an improvement in left ventricular function and an increase in myocardial expression of HIF-1α mRNA and VEGF mRNA following AMI. bFGF may exert its cardioprotective effect through upregulating HIF-1α mRNA and VEGF mRNA in the ischaemic myocardium.
authors.affiliation: Department of Cardiology, Qilu Hospital of Shandong University School of Medicine, Jinan, PR China; Department of Cardiology, Liaocheng People's Hospital, Clinical School of Taishan Medical University, Liaocheng, PR China.
authors.forename: Heng-Chen
authors.initials: HC
authors.lastname: Yao
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Meng
authors.forename: Qian-feng
authors.initials: QF
authors.lastname: Han
authors.forename: Mei
authors.initials: M
authors.lastname: Zhang
authors.forename: Le-Xin
authors.initials: LX
authors.lastname: Wang
mesh_terms: D000818:Animals; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000096:biosynthesis*; D008297:Male; D009124:Muscle Proteins / Q000096:biosynthesis*; D009203:Myocardial Infarction / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis*
chemical_list: C497444:Hif1a protein, rat; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D009124:Muscle Proteins; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat; D016222:Fibroblast Growth Factor 2
keywords: Fibroblast growth factor; Hypoxia-inducible factor; Myocardial infarction; Rats; Vascular endothelial growth factor