title: Immunologic mechanism of Patchouli alcohol anti-H1N1 influenza virus may through regulation of the RLH signal pathway in vitro.
abstract: Patchouli alcohol (PA) is a kind of methanol extracted from traditional Chinese medicine Pogostemonis Herba. Our research aimed to observe the anti-influenza virus role of PA in vitro. 16HBE (human respiratory epithelial cell) was infected by H1N1 (A/FM1/1/47) to set the cell model. Then the 16HBE was co-cultivated with three kinds of immune cells: dendritic cells, macrophages, and monocytes, PA (the concentration is 10 μg/mL) was added as a treatment intervention for 24 h. The immune cells and the supernate were collected for RT-PCR and ELISA detection related to RLH (RIG-1-like helicases) pathway. Results showed that the IL-4 and IFN-γ in supernate were increased after H1N1 infection, and the PA treatment suppressed the expression of cytokines and the mRNA of RLH pathway. PA anti-influenza virus may through regulate the RLH singal pathway.
authors.affiliation: Department of Traditional Chinese Medicine, Medical College of Jinan University, Guangzhou, China.
authors.forename: Xian-Lin
authors.initials: XL
authors.lastname: Wu
authors.forename: Da-Hong
authors.initials: DH
authors.lastname: Ju
authors.forename: Jia
authors.initials: J
authors.lastname: Chen
authors.forename: Bin
authors.initials: B
authors.lastname: Yu
authors.forename: Kang-Li
authors.initials: KL
authors.lastname: Liu
authors.forename: Jin-Xiong
authors.initials: JX
authors.lastname: He
authors.forename: Cong-Qi
authors.initials: CQ
authors.lastname: Dai
authors.forename: Sha
authors.initials: S
authors.lastname: Wu
authors.forename: Zhou
authors.initials: Z
authors.lastname: Chang
authors.forename: You-Ping
authors.initials: YP
authors.lastname: Wang
authors.forename: Xiao-Yin
authors.initials: XY
authors.lastname: Chen
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D007155:Immunologic Factors / Q000494:pharmacology*; D053118:Influenza A Virus, H1N1 Subtype / Q000187:drug effects* / Q000502:physiology; D007251:Influenza, Human / Q000188:drug therapy / Q000201:enzymology / Q000276:immunology* / Q000821:virology; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology; D015847:Interleukin-4 / Q000235:genetics / Q000276:immunology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D020365:RNA Helicases / Q000235:genetics / Q000276:immunology*; D012717:Sesquiterpenes / Q000494:pharmacology*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D004365:Drugs, Chinese Herbal; D007155:Immunologic Factors; D012717:Sesquiterpenes; D015847:Interleukin-4; D007371:Interferon-gamma; D020365:RNA Helicases; C052983:patchouli alcohol