title: Multilayer scaffold of electrospun PLA-PCL-collagen nanofibers as a dural substitute.
abstract: Dural closure after the neurosurgery can prevent postoperative complications. Although many types of dural substitute have been developed, most of them lack functional and structural characteristics compared with the natural dura mater. In this study, we used electrospinning method to fabricate a multilayer scaffold to promote dural repair. The inner layer of the scaffold that faces the brain tissue is composed of poly-lactic acid (PLA) to reduce tissue adhesion. The middle layer of the scaffold is composed of poly-ɛ-caprolactone and PLA, which provides a watertight seal. The outer layer of the scaffold contains a large amount of collagen to promote cell attachment and proliferation. The results from in vitro study and an animal model have shown that this multilayer fibrous scaffold has sufficient mechanic strength and biochemical properties to enhance dural repair. Therefore, fabrication of scaffold with multiple functional and structural layers may provide a novel approach for tissue engineering.
authors.affiliation: Department of Anatomy, Key Lab of Biomechanics and Tissue Engineering Third Military Medical University, Chongqing, 400038, China; Department of Neurosurgery, Kunming Military General Hospital, Kunming, 650000, China.
authors.forename: Yu-fei
authors.initials: YF
authors.lastname: Wang
authors.forename: Hong-feng
authors.initials: HF
authors.lastname: Guo
authors.forename: Da-jun
authors.initials: DJ
authors.lastname: Ying
mesh_terms: D000042:Absorption; D000165:Acridine Orange; D000818:Animals; D002448:Cell Adhesion; D049109:Cell Proliferation; D002470:Cell Survival; D003094:Collagen / Q000737:chemistry*; D004388:Dura Mater / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry; D011817:Rabbits; D013778:Tetrazolium Salts; D013844:Thiazoles; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014867:Water
chemical_list: D011091:Polyesters; D013778:Tetrazolium Salts; D013844:Thiazoles; D014867:Water; C016240:polycaprolactone; D003094:Collagen; C022616:thiazolyl blue; D000165:Acridine Orange
keywords: collagen; dural substitutes; electrospinning; poly-lactic acid; poly-ϵ-caprolactone