title: Sphingomonas kyungheensis sp. nov., a bacterium with ginsenoside-converting activity isolated from soil of a ginseng field.
abstract: A bacterial strain THG-B283(T), which has β-glucosidase activity, was isolated from soil of a ginseng field. Cells were Gram-reaction-negative, oxidase- and catalase-positive, aerobic, motile with one polar flagellum and rod-shaped. The strain was subjected to a polyphasic taxonomic study. Strain THG-B283(T) grew optimally at around pH 7.0, at 25-28 °C and in the absence of NaCl on R2A agar. 16S rRNA gene sequence analysis revealed that strain THG-B283(T) belongs to the family Sphingomonadaceae and is closely related to Sphingomonas melonis DAPP-PG 224(T) (98.2 %), S. aquatilis JSS7(T) (98.1 %), S. insulae DS-28(T) (97.6 %), S. mali IFO 15500(T) (97.1 %) and S. pruni IFO 15498(T) (97.0 %). Strain THG-B283(T) contained Q-10 as the predominant ubiquinone. The major fatty acids included summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), C18 : 1ω7c, C14 : 0 2-OH and C16 : 0. The DNA G+C content was 72.2 mol%. The major component in the polyamine pattern was sym-homospermidine. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidyldimethylethanolamine, phosphatidylcholine, sphingoglycolipid, diphosphatidylglycerol, an unidentified glycolipid, unidentified aminolipids, an unidentified phospholipid and unidentified lipids. Genomic and chemotaxonomic data supported the affiliation of strain THG-B283(T) to the genus Sphingomonas. DNA-DNA relatedness between strain THG-B283(T) and its closest phylogenetic neighbours was below 23 %. On the basis of phenotypic, phylogenetic and genetic data, strain THG-B283(T) represents a novel species of genus Sphingomonas, for which the name Sphingomonas kyungheensis sp. nov. is proposed. The type strain is THG-B283(T) ( = KACC 16224(T) = LMG 26582(T)).
authors.affiliation: Department of Oriental Medicinal Material & Processing College of Life Science, Kyung Hee University, Yongin, Kyunggi 446-701, Republic of Korea.
authors.forename: Heung-Min
authors.initials: HM
authors.lastname: Son
authors.affiliation: Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Republic of Korea.
authors.forename: Jung-Eun
authors.initials: JE
authors.lastname: Yang
authors.affiliation: Department of Oriental Medicinal Material & Processing College of Life Science, Kyung Hee University, Yongin, Kyunggi 446-701, Republic of Korea.
authors.forename: YongJin
authors.initials: Y
authors.lastname: Park
authors.affiliation: Department of Oriental Medicinal Material & Processing College of Life Science, Kyung Hee University, Yongin, Kyunggi 446-701, Republic of Korea.
authors.forename: Chang-Kyun
authors.initials: CK
authors.lastname: Han
authors.affiliation: Microbial Resource Center, KCTC, Korea Research Institute of Bioscience & Biotechnology, Daejeon 305-806, Republic of Korea.
authors.forename: Song-Gun
authors.initials: SG
authors.lastname: Kim
authors.affiliation: Department of Marine Biotechnology, Anyang University, Incheon 417-833, Republic of Korea.
authors.forename: MooChang
authors.initials: M
authors.lastname: Kook
authors.affiliation: Department of Oriental Medicinal Material & Processing College of Life Science, Kyung Hee University, Yongin, Kyunggi 446-701, Republic of Korea.
authors.forename: Tae-Hoo
authors.initials: TH
authors.lastname: Yi
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D005894:Panax / Q000382:microbiology*; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013095:Spermidine / Q000031:analogs & derivatives / Q000032:analysis; D020578:Sphingomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014451:Ubiquinone / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; C007961:sym-homospermidine; D014451:Ubiquinone; C025204:Ubiquinone Q2; D013095:Spermidine