title: PPISURV: a novel bioinformatics tool for uncovering the hidden role of specific genes in cancer survival outcome.
abstract: Multiple clinical studies have correlated gene expression with survival outcome in cancer on a genome-wide scale. However, in many cases, no obvious correlation between expression of well-known tumour-related genes (that is, p53, p73 and p21) and survival rates of patients has been observed. This can be mainly explained by the complex molecular mechanisms involved in cancer, which mask the clinical relevance of a gene with multiple functions if only gene expression status is considered. As we demonstrate here, in many such cases, the expression of the gene interaction partners (gene 'interactome') correlates significantly with cancer survival and is indicative of the role of that gene in cancer. On the basis of this principle, we have implemented a free online datamining tool (http://www.bioprofiling.de/PPISURV). PPISURV automatically correlates expression of an input gene interactome with survival rates on >40 publicly available clinical expression data sets covering various tumours involving about 8000 patients in total. To derive the query gene interactome, PPISURV employs several public databases including protein-protein interactions, regulatory and signalling pathways and protein post-translational modifications.
authors.affiliation: 1] Toxicology Unit, Hodgkin Building, Medical Research Council, Leicester University, Leicester, UK [2] Molecular Pharmacology Laboratory, Technological University, St-Petersburg, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Antonov
authors.affiliation: Institute for Molecular Medicine Finland FIMM, University of Helsinki; Helsinki, Finland.
authors.forename: M
authors.initials: M
authors.lastname: Krestyaninova
authors.affiliation: Toxicology Unit, Hodgkin Building, Medical Research Council, Leicester University, Leicester, UK.
authors.forename: R A
authors.initials: RA
authors.lastname: Knight
authors.affiliation: Donnelly Center for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, Canada.
authors.forename: I
authors.initials: I
authors.lastname: Rodchenkov
authors.affiliation: 1] Toxicology Unit, Hodgkin Building, Medical Research Council, Leicester University, Leicester, UK [2] Molecular Pharmacology Laboratory, Technological University, St-Petersburg, Russia [3] Department of Experimental Medicine and Surgery, University of Rome 'Tor Vergata', Via Montpellier 1, Rome, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Melino
authors.affiliation: 1] Molecular Pharmacology Laboratory, Technological University, St-Petersburg, Russia [2] Department of Biochemistry, University of Leicester, Leicester, UK [3] Gene Expression Programme, Institute of Cytology, St-Petersburg, Russia.
authors.forename: N A
authors.initials: NA
authors.lastname: Barlev
mesh_terms: D019295:Computational Biology / Q000379:methods*; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D020869:Gene Expression Profiling; D016158:Genes, p53; D006801:Humans; D009369:Neoplasms / Q000235:genetics*; D009687:Nuclear Proteins / Q000235:genetics; D015996:Survival Rate; D016896:Treatment Outcome; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins / Q000235:genetics
chemical_list: C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C000606460:TP73 protein, human; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins