title: Changes of apoptosis in tumor tissues with time after irreversible electroporation.
abstract: Irreversible electroporation is a novel method of ablating living tissues through its non-thermal effects, unlike radiofrequency ablation which has a severe problem of heat sink. It is due to high-energy direct current which leads to permanent disruption of lipid bilayer integrity in terms of exchanges between intra- and extracellular components via nano-sized pores. That finally causes irreversible damage to cellular homeostasis. Irreversibly damaged cells may undergo apoptosis followed by necrosis with time after electroporation. This damage can make it possible to monitor the ablated area with time post-IRE through MR imaging and an ultrasound system. Most previous studies have investigated the immediate response of undesired tissue to IRE. In our study, we showed changes of tumor tissues with time post-IRE by histological analysis and MR imaging. Tissues under IRE ablation showed a peak apoptotic rate at 24 h after IRE ablation with viable tissues at the peripheral rim of treated tissues in histological analysis. This phenomenon was also observed with no enhancement on contrast-enhanced MR images due to devascularization of IRE ablated zones.
authors.affiliation: Department of Radiology and Seoul National University Cancer Research Institute, Seoul National University College of Medicine, SNU-SMG Boramae Medical Center, 39 Boramae-Gil, Dongjak-Gu, Seoul 156-707, Republic of Korea.
authors.forename: Hong-Bae
authors.initials: HB
authors.lastname: Kim
authors.forename: Chang-Kyu
authors.initials: CK
authors.lastname: Sung
authors.forename: Ku Youn
authors.initials: KY
authors.lastname: Baik
authors.forename: Kee-Wook
authors.initials: KW
authors.lastname: Moon
authors.forename: Hyung-Sik
authors.initials: HS
authors.lastname: Kim
authors.forename: Jeong-Han
authors.initials: JH
authors.lastname: Yi
authors.forename: Jong-Hyun
authors.initials: JH
authors.lastname: Jung
authors.forename: Min-Hoan
authors.initials: MH
authors.lastname: Moon
authors.forename: Ohc-Kyung
authors.initials: OK
authors.lastname: Choi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D045744:Cell Line, Tumor; D018274:Electroporation / Q000379:methods*; D005260:Female; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011471:Prostatic Neoplasms / Q000473:pathology* / Q000503:physiopathology / Q000628:therapy*; D016896:Treatment Outcome