title: [Determination of plasma protein binding rate of arctiin and arctigenin with ultrafiltration].
abstract: OBJECTIVE
  To determine the plasma protein binding rate of arctiin and arctigenin.
  
  
  METHODS
  The ultrafiltration combined with HPLC was employed to determine the plasma protein binding rate of arctiin and arctigenin as well as rat plasma and healthy human plasma proteins.
  
  
  RESULTS
  The plasma protein binding rate of arctiin with rat plasma at the concentrations of 64. 29, 32.14, 16.07 mg x L(-1) were (71.2 +/- 2.0)%, (73.4 +/- 0.61)%, (78.2 +/- 1.9)%, respectively; while the plasma protein binding rate of arctiin with healthy human plasma at the above concentrations were (64.8 +/- 3.1)%, (64.5 +/- 2.5)%, (77.5 +/- 1.7)%, respectively. The plasma protein binding rate of arctigenin with rat plasma at the concentrations of 77.42, 38.71, 19.36 mg x L(-1) were (96.7 +/- 0.41)%, (96.8 +/- 1.6)%, (97.3 +/- 0.46)%, respectively; while the plasma protein binding rate of arctigenin with normal human plasma at the above concentrations were (94.7 +/- 3.1)%, (96.8 +/- 1.6)%, (97.9 +/- 1.3)%, respectively.
  
  
  CONCLUSIONS
  The binding rate of arctiin with rat plasma protein was moderate, which is slightly higher than the binding rate of arctiin with healthy human plasma protein. The plasma protein binding rates of arctigenin with both rat plasma and healthy human plasma are very high.
authors.affiliation: School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China. hxy4848@126.com
authors.forename: Xue-Ying
authors.initials: XY
authors.lastname: Han
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Ri-Qiu
authors.initials: RQ
authors.lastname: Tan
authors.forename: De-Qiang
authors.initials: DQ
authors.lastname: Dou
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D001798:Blood Proteins / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005663:Furans / Q000378:metabolism*; D005960:Glucosides / Q000378:metabolism*; D006801:Humans; D017705:Lignans / Q000378:metabolism*; D008297:Male; D011485:Protein Binding; D051381:Rats; D017207:Rats, Sprague-Dawley; D014462:Ultrafiltration / Q000379:methods*
chemical_list: D001798:Blood Proteins; D005663:Furans; D005960:Glucosides; D017705:Lignans; C077992:arctiin; C071942:arctigenin