title: Comparison of sealing ability of MTA and EndoSequence Bioceramic Root Repair Material: a bacterial leakage study.
abstract: OBJECTIVE
  To compare the sealing ability of ProRoot mineral trioxide aggregate (MTA) to the sealing ability of EndoSequence Bioceramic Root Repair Material (ES-BCRR) putty using a bacterial leakage model.
  
  
  METHODS
  Root canals of 60 single-rooted extracted teeth were enlarged to an apical diameter of 0.5 mm using EndoSequence files. The apical 3 mm of each root was sectioned at 90 degrees to the long axis of the root. An ultrasonic surgical tip was used to prepare a 3-mm deep root-end preparation in all teeth. Teeth were equally divided into four groups: Group 1, MTA; Group 2, ES-BCRR putty; Group 3, positive control, gutta-percha without sealer; Group 4, negative control, sealed with wax and nail varnish. Prepared teeth were kept moist for 48 hours to allow for initial setting of the materials. After ethylene oxide sterilization, the teeth were suspended in sterilized vials containing 3% phenol lactose broth and inoculated with Enterococcus faecalis through the occlusal access openings. The samples were observed daily for leakage to a maximum of 28 days. Chi-square and Fisher exact tests were used to compare the experimental groups and an alpha level of significance was set at P = .05.
  
  
  RESULTS
  In the ES-BCRR group 93% of samples leaked, compared to only 20% of samples in the MTA group. There was a significant difference in leakage between the experimental groups (P < .0001). Also there were no significant differences between the negative control group and MTA group and between the positive control group and ES-BCRR group (P = 1.00).
  
  
  CONCLUSIONS
  Samples in the ES-BCRR group leaked significantly more than samples in the MTA group.
authors.affiliation: Department of Endodontics, University of Medicine and Dentistry of New Jersey, New Jersey Dental School, Newark, New Jersey 07101, USA. hirschcs@umdnj.edu
authors.forename: Craig S
authors.initials: CS
authors.lastname: Hirschberg
authors.forename: Niyati S
authors.initials: NS
authors.lastname: Patel
authors.forename: Loken M
authors.initials: LM
authors.lastname: Patel
authors.forename: Daniel E
authors.initials: DE
authors.lastname: Kadouri
authors.forename: Gary R
authors.initials: GR
authors.lastname: Hartwell
mesh_terms: D017607:Aluminum Compounds; D017610:Calcium Compounds; D002130:Calcium Phosphates; D016009:Chi-Square Distribution; D003763:Dental Leakage / Q000382:microbiology / Q000517:prevention & control*; D004338:Drug Combinations; D013293:Enterococcus faecalis; D006801:Humans; D010087:Oxides; D012387:Root Canal Filling Materials; D012389:Root Canal Obturation; D018915:Root Canal Preparation; D017640:Silicates
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D002130:Calcium Phosphates; D004338:Drug Combinations; C550603:EndoSequence root repair material; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate