title: Cultivation of limbal epithelial cells on electrospun poly (lactide-co-glycolide) scaffolds for delivery to the cornea.
abstract: In delivering tissues to the body, both natural and synthetic materials have been used. Currently, a natural membrane, the human amniotic membrane (AM), is used to deliver limbal epithelial cells (LEC) to the cornea. AM presents inherent problems with structural variation and requires extensive serological screening before use. Therefore alternatives are required to improve the predictability in clinical outcomes and economic costs associated with the use of this biological substrate. In this chapter, we describe the development of an alternative, structurally simple, synthetic biodegradable electrospun scaffold based on poly(lactide-co-glycolide) (PLGA: materials used in dissolvable sutures) to replace AM.
authors.affiliation: The Kroto Research Institute, University of Sheffield, Sheffield, UK.
authors.forename: Pallavi
authors.initials: P
authors.lastname: Deshpande
authors.forename: Charanya
authors.initials: C
authors.lastname: Ramachandran
authors.forename: Virender S
authors.initials: VS
authors.lastname: Sangwan
authors.forename: Sheila
authors.initials: S
authors.lastname: Macneil
mesh_terms: D016475:3T3 Cells; D020341:Absorbable Implants; D000818:Animals; D018920:Coculture Techniques; D003316:Corneal Diseases / Q000628:therapy; D004847:Epithelial Cells / Q000166:cytology* / Q000637:transplantation; D061252:Feeder Cells; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D016850:Limbus Corneae / Q000166:cytology*; D051379:Mice; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid