title: Development of a chloroplast DNA marker for monitoring of transgene introgression in Brassica napus L.
abstract: Chloroplast molecular markers can provide useful information for high-resolution analysis of inter- and intra-specific variation in Brassicaceae and for differentiation between its species. Combining data generated from nuclear and chloroplast markers enables the study of seed and pollen movement, and assists in the assessment of gene-flow from genetically modified (GM) plants through hybridization studies. To develop chloroplast DNA markers for monitoring of transgene introgression in Brassica napus L., we searched for sequence variations in the chloroplast (cp) genome, and developed a simple cpDNA marker that is reliable, time-saving, and easily discriminates among 4 species (B. napus, B. rapa, Raphanus sativus, and Sinapis alba) based on PCR-product length polymorphism. This marker will be useful to identify maternal lineages and to estimate transgene movement of GM canola.
authors.affiliation: Biosafety Division, National Academy of Agricultural Science, Rural Development Administration, Suwon, 441-707, South Korea. woo001@korea.kr
authors.forename: Hee-Jong
authors.initials: HJ
authors.lastname: Woo
authors.forename: Myung-Ho
authors.initials: MH
authors.lastname: Lim
authors.forename: Kong-Sik
authors.initials: KS
authors.lastname: Shin
authors.forename: Bianca
authors.initials: B
authors.lastname: Martins
authors.forename: Bum-Kyu
authors.initials: BK
authors.lastname: Lee
authors.forename: Hyun-Suk
authors.initials: HS
authors.lastname: Cho
authors.forename: Carol A
authors.initials: CA
authors.lastname: Mallory-Smith
mesh_terms: D029688:Brassica napus / Q000145:classification* / Q000235:genetics*; D018742:DNA, Chloroplast / Q000235:genetics*; D005819:Genetic Markers; D014644:Genetic Variation; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction / Q000379:methods; D031226:Sinapis / Q000145:classification / Q000235:genetics; D019076:Transgenes
chemical_list: D018742:DNA, Chloroplast; D005819:Genetic Markers