title: Phytochemical and Antimicrobial Studies of Methyl Angolensate and Luteolin-7-O-glucoside Isolated from Callus Cultures of Soymida febrifuga.
abstract: Soymida febrifuga (Roxb.) A. Juss. is an indigenous lofty deciduous medicinal tree, monotypic genus endemic to India. Hexane, ethyl acetate and methanol extracts of Soymida febrifuga root callus were tested for their phytochemical constituents and antimicrobial activity. Among them, ethyl acetate extract was found to be most effective, which on subjection to silica gel column chromatography led to the separation and isolation of methyl angolensate and luteolin-7-O-glucoside. Structures were determined by Nuclear Magnetic Resonance and Liquid Chromatographic Mass Spectroscopic methods. Further studies indicated that methyl angolensate and luteolin-7-O-glucoside had an anti-bacterial effect against Bacillus subtilis and Salmonella typhimurium, respectively. In addition to that methyl angolensate had an anti-fungal activity against Aspergillus niger while luteolin-7-O-glucoside inhibited Alternaria alternata.
authors.affiliation: Division of Plant Tissue Culture, Department of Botany, Sri Venkateswara University, Tirupati-517502, Andhra Pradesh, India; ; Department of Biochemistry, Indian Institute of Science, Bangalore, India.
authors.forename: Kishore K
authors.initials: KK
authors.lastname: Chiruvella
authors.forename: Arifullah
authors.initials: A
authors.lastname: Mohammed
authors.forename: Gayathri
authors.initials: G
authors.lastname: Dampuri
authors.forename: Rama Gopal
authors.initials: RG
authors.lastname: Ghanta
authors.forename: Sathees C
authors.initials: SC
authors.lastname: Raghavan
keywords: Meliaceae; Soymida febrifuga; antibacterial; antifungal; callus; luteolin-7-O-glucoside; methyl angolensate; silica gel column chromatography