title: Immunogenicity of dormancy-related antigens in individuals infected with Mycobacterium tuberculosis in Japan.
abstract: METHODS
  DosR regulon genes are considered essential for Mycobacterium tuberculosis dormancy, and their products are demonstrated to have immunogenicity in M. tuberculosis-infected individuals, suggesting that DosR regulon-encoded proteins are suitable targets for vaccines to control the reactivation of dormant M. tuberculosis.
  
  
  OBJECTIVE
  Prospective analysis of T-cell and antibody responses against DosR regulon-encoded antigens in M. tuberculosis-infected individuals in Japan to identify effective vaccine targets.
  
  
  METHODS
  T-cell responses against 33 DosR regulon-encoded antigens were investigated in 26 consecutive M. tuberculosis-infected individuals--14 with latent tuberculosis infection (LTBI) and 12 with active pulmonary tuberculosis (PTB)--using enzyme-linked immunosorbent spot assay, and antibody responses in 42 consecutive individuals, 14 with LTBI and 28 with PTB.
  
  
  RESULTS
  Six antigens (Rv0570, Rv1996, Rv2004c, Rv2028c, Rv2029c and Rv3133c) induced stronger T-cell responses in LTBI than in PTB, In contrast, antigen-specific antibody responses to five antigens (Rv0080, Rv1738, Rv2007c, Rv2031c and Rv2032) were found to be stronger in PTB than in LTBI cases.
  
  
  CONCLUSIONS
  T-cell responses to six antigens might contribute to natural protection against dormant M. tuberculosis. These antigens are therefore considered to be potential targets of novel vaccines to control M. tuberculosis reactivation in the Japanese population.
authors.affiliation: Department of Infectious Diseases, and Internal Medicine 2 (Divisions of Endocrinology & Metabolism, Respiratology & Hepatology), Hamamatsu University School of Medicine, Hamamatsu, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Hozumi
authors.forename: K
authors.initials: K
authors.lastname: Tsujimura
authors.forename: Y
authors.initials: Y
authors.lastname: Yamamura
authors.forename: S
authors.initials: S
authors.lastname: Seto
authors.forename: M
authors.initials: M
authors.lastname: Uchijima
authors.forename: T
authors.initials: T
authors.lastname: Nagata
authors.forename: S
authors.initials: S
authors.lastname: Miwa
authors.forename: H
authors.initials: H
authors.lastname: Hayakawa
authors.forename: T
authors.initials: T
authors.lastname: Fujisawa
authors.forename: D
authors.initials: D
authors.lastname: Hashimoto
authors.forename: N
authors.initials: N
authors.lastname: Inui
authors.forename: T
authors.initials: T
authors.lastname: Suda
authors.forename: K
authors.initials: K
authors.lastname: Chida
authors.forename: Y
authors.initials: Y
authors.lastname: Koide
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000917:Antibody Formation / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology*; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology; D004268:DNA-Binding Proteins; D058501:Enzyme-Linked Immunospot Assay; D005260:Female; D006801:Humans; D007564:Japan; D055985:Latent Tuberculosis / Q000235:genetics / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000276:immunology*; D011446:Prospective Studies; D011494:Protein Kinases / Q000235:genetics / Q000276:immunology; D018085:Regulon / Q000235:genetics / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology; D014397:Tuberculosis, Pulmonary / Q000235:genetics / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C542863:DosR protein, Mycobacterium tuberculosis; D011494:Protein Kinases