title: Adipose tissue-derived stem cells as a regenerative therapy for a mouse steatohepatitis-induced cirrhosis model.
abstract: Cirrhosis is a chronic liver disease that impairs hepatic function and causes advanced fibrosis. Mesenchymal stem cells have gained recent popularity as a regenerative therapy since they possess immunomodulatory functions. We found that injected adipose tissue-derived stem cells (ADSCs) reside in the liver. Injection of ADSCs also restores albumin expression in hepatic parenchymal cells and ameliorates fibrosis in a nonalcoholic steatohepatitis model of cirrhosis in mice. Gene expression analysis of the liver identifies up- and down-regulation of genes, indicating regeneration/repair and anti-inflammatory processes following ADSC injection. ADSC treatment also decreases the number of intrahepatic infiltrating CD11b(+) and Gr-1(+) cells and reduces the ratio of CD8(+) /CD4(+) cells in hepatic inflammatory cells. This is consistent with down-regulation of genes in hepatic inflammatory cells related to antigen presentation and helper T-cell activation.
  
  
  CONCLUSIONS
  These results suggest that ADSC therapy is beneficial in cirrhosis, as it can repair and restore the function of the impaired liver.
authors.affiliation: Department of Gastroenterology, Kanazawa University Hospital, Ishikawa, Japan; Disease Control and Homeostasis, Kanazawa University, Ishikawa, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Seki
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Sakai
authors.forename: Takuya
authors.initials: T
authors.lastname: Komura
authors.forename: Alessandro
authors.initials: A
authors.lastname: Nasti
authors.forename: Keiko
authors.initials: K
authors.lastname: Yoshida
authors.forename: Mami
authors.initials: M
authors.lastname: Higashimoto
authors.forename: Masao
authors.initials: M
authors.lastname: Honda
authors.forename: Soichiro
authors.initials: S
authors.lastname: Usui
authors.forename: Masayuki
authors.initials: M
authors.lastname: Takamura
authors.forename: Toshinari
authors.initials: T
authors.lastname: Takamura
authors.forename: Takahiro
authors.initials: T
authors.lastname: Ochiya
authors.forename: Kengo
authors.initials: K
authors.lastname: Furuichi
authors.forename: Takashi
authors.initials: T
authors.lastname: Wada
authors.forename: Shuichi
authors.initials: S
authors.lastname: Kaneko
mesh_terms: D000273:Adipose Tissue / Q000473:pathology*; D000418:Albumins / Q000378:metabolism; D000818:Animals; D039481:CD11b Antigen / Q000378:metabolism; D016516:CD4-CD8 Ratio; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005234:Fatty Liver / Q000150:complications*; D005260:Female; D008103:Liver Cirrhosis / Q000209:etiology* / Q000628:therapy*; D008115:Liver Regeneration / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D019707:Receptors, Chemokine / Q000378:metabolism; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000473:pathology*; D016896:Treatment Outcome
chemical_list: D000418:Albumins; D039481:CD11b Antigen; C503133:Gr-1 protein, mouse; D019707:Receptors, Chemokine