title: Microfluidic amperometric sensor for analysis of nitric oxide in whole blood.
abstract: Standard photolithographic techniques and a nitric oxide (NO) selective xerogel polymer were utilized to fabricate an amperometric NO microfluidic sensor with low background noise and the ability to analyze NO levels in small sample volumes (~250 μL). The sensor exhibited excellent analytical performance in phosphate buffered saline, including a NO sensitivity of 1.4 pA nM(-1), a limit of detection (LOD) of 840 pM, and selectivity over nitrite, ascorbic acid, acetaminophen, uric acid, hydrogen sulfide, ammonium, ammonia, and both protonated and deprotonated peroxynitrite (selectivity coefficients of -5.3, -4.2, -4.0, -5.0, -6.0, -5.8, -3.8, -1.5, and -4.0, respectively). To demonstrate the utility of the microfluidic NO sensor for biomedical analysis, the device was used to monitor changes in blood NO levels during the onset of sepsis in a murine pneumonia model.
authors.affiliation: Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.
authors.forename: Rebecca A
authors.initials: RA
authors.lastname: Hunter
authors.forename: Benjamin J
authors.initials: BJ
authors.lastname: Privett
authors.forename: W Hampton
authors.initials: WH
authors.lastname: Henley
authors.forename: Elise R
authors.initials: ER
authors.lastname: Breed
authors.forename: Zhe
authors.initials: Z
authors.lastname: Liang
authors.forename: Rohit
authors.initials: R
authors.lastname: Mittal
authors.forename: Benyam P
authors.initials: BP
authors.lastname: Yoseph
authors.forename: Jonathan E
authors.initials: JE
authors.lastname: McDunn
authors.forename: Eileen M
authors.initials: EM
authors.lastname: Burd
authors.forename: Craig M
authors.initials: CM
authors.lastname: Coopersmith
authors.forename: J Michael
authors.initials: JM
authors.lastname: Ramsey
authors.forename: Mark H
authors.initials: MH
authors.lastname: Schoenfisch
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D044085:Microfluidics / Q000379:methods*; D009569:Nitric Oxide / Q000032:analysis / Q000097:blood*; D013552:Swine
chemical_list: D009569:Nitric Oxide