title: [Synthesis and identification of rutin complete antigen and analysis its immunogenicity].
abstract: OBJECTIVE
  Synthesis and identification of complete antigen of rutin, the traditional Chinese medicine active ingredient, and develop rapid detection of rutin using enzyme-linked immunoassay method (ELISA). Immunogenicity of the complete antigen was also studied.
  
  
  METHODS
  Prepare the complete antigen by sodium periodate solution and identified by UV scanning and SDS-PAGE test. Male New Zealand white rabbits were immunized by the antigen to obtain the antiserum.
  
  
  RESULTS
  The results of UV analysis showed that the coupling ratio of complete antigen is 13: 1. SDS-PAGE display of the artificial antigen was delayed compared with bovine serum protein. The titer of rutin antibody is 1:4 000. The sensitivity of IC50 was 5.37 mg x L(-1), the lowest detection limit was 1 mg x L(-1), the average recovery was 102%, the intra and interspecific RSD were less than 10%, cross-reactivity rate of antibodies and other analogs were less than 1%.
  
  
  CONCLUSIONS
  Rutin complete antigen was synthesized successfully, and the rapid detection of rutin by ELISA method was successfully established.
authors.affiliation: Department of Pharmaceutics, Hunan University of Traditional Chinese Medicine, Changsha 410208, China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Bao
authors.forename: Fu-Yuan
authors.initials: FY
authors.lastname: He
authors.forename: Jiao-Li
authors.initials: JL
authors.lastname: Zeng
authors.forename: Zan-Shao
authors.initials: ZS
authors.lastname: Xia
authors.forename: Jun-Lin
authors.initials: JL
authors.lastname: Deng
authors.forename: Qing-Hui
authors.initials: QH
authors.lastname: Sun
authors.forename: Hong
authors.initials: H
authors.lastname: Lei
authors.forename: Yu-Ran
authors.initials: YR
authors.lastname: Du
mesh_terms: D000818:Animals; D000918:Antibody Specificity / Q000276:immunology*; D000941:Antigens / Q000276:immunology*; D002417:Cattle; D003429:Cross Reactions / Q000276:immunology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D007106:Immune Sera / Q000276:immunology*; D007114:Immunization; D008297:Male; D010504:Periodic Acid / Q000737:chemistry; D011817:Rabbits; D012431:Rutin / Q000138:chemical synthesis / Q000276:immunology*; D012710:Serum Albumin, Bovine / Q000276:immunology; D012996:Solutions / Q000737:chemistry
chemical_list: D000941:Antigens; D007106:Immune Sera; D012996:Solutions; D010504:Periodic Acid; D012710:Serum Albumin, Bovine; D012431:Rutin; C009288:metaperiodate