title: Mass spectrometry-based metabolomics of single yeast cells.
abstract: Single-cell level measurements are necessary to characterize the intrinsic biological variability in a population of cells. In this study, we demonstrate that, with the microarrays for mass spectrometry platform, we are able to observe this variability. We monitor environmentally (2-deoxy-D-glucose) and genetically (ΔPFK2) perturbed Saccharomyces cerevisiae cells at the single-cell, few-cell, and population levels. Correlation plots between metabolites from the glycolytic pathway, as well as with the observed ATP/ADP ratio as a measure of cellular energy charge, give biological insight that is not accessible from population-level metabolomic data.
authors.affiliation: Department of Chemistry and Applied Biosciences, Eidgenössiche Technische Hochschule Zurich, CH-8093 Zurich, Switzerland.
authors.forename: Alfredo J
authors.initials: AJ
authors.lastname: Ibáñez
authors.forename: Stephan R
authors.initials: SR
authors.lastname: Fagerer
authors.forename: Anna Mareike
authors.initials: AM
authors.lastname: Schmidt
authors.forename: Pawel L
authors.initials: PL
authors.lastname: Urban
authors.forename: Konstantins
authors.initials: K
authors.lastname: Jefimovs
authors.forename: Philipp
authors.initials: P
authors.lastname: Geiger
authors.forename: Reinhard
authors.initials: R
authors.lastname: Dechant
authors.forename: Matthias
authors.initials: M
authors.lastname: Heinemann
authors.forename: Renato
authors.initials: R
authors.lastname: Zenobi
mesh_terms: D002452:Cell Count; D003847:Deoxyglucose; D006019:Glycolysis / Q000502:physiology*; D016014:Linear Models; D055432:Metabolomics / Q000379:methods*; D046228:Microarray Analysis / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000378:metabolism / Q000502:physiology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D003847:Deoxyglucose
keywords: MALDI mass spectrometry; baker's yeast; single-cell measurements