title: Identification and production of a novel natural pigment, cordycepoid A, from Cordyceps bifusispora.
abstract: A novel yellow pigment, cordycepoid A, was isolated and identified from the entomogenous fungi Cordyceps bifusispora. Cordycepoid A exhibited no significant toxicity against Chinese hamster ovary (CHO) cells and mice, and showed high stability against food addictives, metal ions and heat. A liquid/solid double-phase cultural process for the production of the pigment was optimized as follows: 3 days aged liquid seed, 7.5 % inoculums, incubation temperature at 25 °C, 10 days of solid culture, and the last 5 days exposed to 200 Lx scattered light. The liquid seed medium and the solid culture medium were also optimized. Ethanol was selected as extracting solvent for its scale-up production. The optimal extracting conditions were determined as liquid/solid ratio at 20:1, extracting temperature at 40 °C, ultrasonic power at 400 W, and extracting time of 40 min.
authors.affiliation: Anhui Agricultural University, Hefei 230036, China.
authors.forename: Rui-Li
authors.initials: RL
authors.lastname: Lu
authors.forename: Fei-Fei
authors.initials: FF
authors.lastname: Luo
authors.forename: Feng-Lin
authors.initials: FL
authors.lastname: Hu
authors.forename: Bo
authors.initials: B
authors.lastname: Huang
authors.forename: Chun-Ru
authors.initials: CR
authors.lastname: Li
authors.forename: Guan-Hu
authors.initials: GH
authors.lastname: Bao
mesh_terms: D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D032661:Cordyceps / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media / Q000378:metabolism; D006801:Humans; D051379:Mice; D015394:Molecular Structure; D010860:Pigments, Biological / Q000096:biosynthesis* / Q000737:chemistry* / Q000302:isolation & purification / Q000633:toxicity; D012995:Solubility
chemical_list: D003470:Culture Media; D010860:Pigments, Biological