title: Comparative studies on the anti-tumor activities of high temperature- and pH-modified citrus pectins.
abstract: High temperature and pH modification could produce functional pectins. In this study, high temperature-modified (HTCP) and pH-modified (MCP) citrus pectins were prepared for studying their anti-tumor activities in eight cancer cell lines and a mouse Sarcoma-180 (S-180) tumor model. HTCP inhibited the proliferation of these cancer cells and induced a caspase-3-dependent cell apoptosis and cell cycle arrest at G2/M phase. It also inhibited the growth of S-180 tumor to 49% of the control at the dose of 200 mg kg(-1) d(-1) and extended the survival time of the tumor-bearing mice. MCP had no anti-proliferative effects on these cancer cells and no anti-tumor effect in the mouse model. The anti-tumor activity of HTCP in the mouse tumor model was not correlated with immunomodulation and galectin-3 inhibition, but correlated well with proliferation inhibition. HTCP might be exploited as a functional food for cancer prevention and/or treatment.
authors.affiliation: School of Life Science, Northeast Normal University, 5268 Renmin Street, 130024 Changchun, Jilin Province, People's Republic of China.
authors.forename: Miao
authors.initials: M
authors.lastname: Hao
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Yuan
authors.forename: Hairong
authors.initials: H
authors.lastname: Cheng
authors.forename: Huiting
authors.initials: H
authors.lastname: Xue
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.forename: Yifa
authors.initials: Y
authors.lastname: Zhou
authors.forename: Guihua
authors.initials: G
authors.lastname: Tai
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009369:Neoplasms / Q000188:drug therapy* / Q000201:enzymology / Q000235:genetics / Q000503:physiopathology; D010368:Pectins / Q000008:administration & dosage* / Q000737:chemistry* / Q000302:isolation & purification; D010936:Plant Extracts / Q000008:administration & dosage* / Q000737:chemistry* / Q000302:isolation & purification; D013696:Temperature
chemical_list: D010936:Plant Extracts; D010368:Pectins; D053148:Caspase 3