title: Biochemical characterization and cooperation with co-chaperones of heat shock protein 90 from Schizosaccharomyces pombe.
abstract: The characterization of Hsp90 from the fission yeast Schizosaccharomyces pombe was performed. Hsp90 of S. pombe existed as a dimer and exhibited ATP-dependent conformational changes. It captured unfolded proteins in the ATP-free open conformation and protected them from thermal aggregation. Hsp90 of S. pombe was also able to refold thermally denatured firefly luciferase. The co-chaperones Sti1 and Aha1 bound Hsp90 and modulated its activity. Because the affinity of Sti1 was higher than that of Aha1, the effect of Sti1 appeared to dominate when both co-chaperones existed simultaneously.
authors.affiliation: Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan.
authors.forename: Mari
authors.initials: M
authors.lastname: Ishida
authors.forename: Taichi
authors.initials: T
authors.lastname: Tomomari
authors.forename: Taro
authors.initials: T
authors.lastname: Kanzaki
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Abe
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Oka
authors.forename: Masafumi
authors.initials: M
authors.lastname: Yohda
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism / Q000494:pharmacology; D018833:Chaperonins / Q000378:metabolism; D018841:HSP90 Heat-Shock Proteins / Q000737:chemistry* / Q000378:metabolism*; D049409:Luciferases, Firefly / Q000737:chemistry / Q000378:metabolism; D018832:Molecular Chaperones / Q000378:metabolism*; D011485:Protein Binding; D011487:Protein Conformation / Q000187:drug effects; D011489:Protein Denaturation; D055503:Protein Multimerization; D058849:Protein Refolding; D058767:Protein Unfolding; D012568:Schizosaccharomyces / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism; D013696:Temperature
chemical_list: C587646:Aha1 protein, S pombe; D018841:HSP90 Heat-Shock Proteins; D018832:Molecular Chaperones; D029702:Schizosaccharomyces pombe Proteins; C587647:Sti1 protein, S pombe; D000255:Adenosine Triphosphate; D049409:Luciferases, Firefly; D018833:Chaperonins
keywords: Chaperone; Co-chaperone; Conformational change; Hsp90; Schizosaccharomyces pombe