title: Decreased L-tryptophan concentration in distinctive brain regions of mice treated repeatedly with phencyclidine.
abstract: It has been reported that repeated phencyclidine (PCP) treatment induces schizophrenia-like behavior in mice. L-Tryptophan (Trp) concentrations in brain tissues of control (n = 8) and PCP-treated mice (10 mg/kg/day, s.c., 14 days, n = 10) were determined using high-performance liquid chromatography (HPLC) with fluorescence detection. The HPLC method involved pre-column fluorescence derivatization with (R)-(-)-4-(N,N-dimethylaminosulfonyl)-7-(3-isothiocyanatopyrrolidin-1-yl)-2,1,3-benzoxadiazole (DBD-PyNCS). Eight different parts of the brain, namely, the frontal cortex, nucleus accumbens, striatum, hippocampus, amygdala, thalamus, hypothalamus, and cerebellum, of both groups were investigated. A significant decrease in the L-Trp concentration in the nucleus accumbens (p = 0.024) and hippocampus (p = 0.027) was observed in PCP-treated mice, suggesting that alteration of the L-Trp metabolism might occur in these brain parts.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Toho University, 2-2-1 Miyama, Funabashi-shi, Chiba, 274-8510, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Iizuka
authors.forename: Shoko
authors.initials: S
authors.lastname: Watanabe
authors.forename: Mayuko
authors.initials: M
authors.lastname: Koshikawa
authors.forename: Yurie
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: Yuki
authors.initials: Y
authors.lastname: Aoyama
authors.forename: Hideaki
authors.initials: H
authors.lastname: Ichiba
authors.forename: Toshitaka
authors.initials: T
authors.lastname: Nabeshima
authors.forename: Takeshi
authors.initials: T
authors.lastname: Fukushima
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D018691:Excitatory Amino Acid Antagonists / Q000009:adverse effects / Q000494:pharmacology*; D017879:Isothiocyanates; D008297:Male; D051379:Mice; D010069:Oxadiazoles; D010622:Phencyclidine / Q000009:adverse effects / Q000494:pharmacology*; D012559:Schizophrenia / Q000139:chemically induced; D014364:Tryptophan / Q000032:analysis / Q000378:metabolism*
chemical_list: C107817:4-(3-isothiocyanatopyrrolidin-1-yl)-7-(N,N-dimethylaminosulfonyl)-2,1,3-benzoxadiazole; D018691:Excitatory Amino Acid Antagonists; D017879:Isothiocyanates; D010069:Oxadiazoles; D014364:Tryptophan; D010622:Phencyclidine