title: Assessment and in vivo scoring of murine experimental autoimmune uveoretinitis using optical coherence tomography.
abstract: Despite advances in clinical imaging and grading our understanding of retinal immune responses and their morphological correlates in experimental autoimmune uveoretinitis (EAU), has been hindered by the requirement for post-mortem histology. To date, monitoring changes occurring during EAU disease progression and evaluating the effect of therapeutic intervention in real time has not been possible. We wanted to establish whether optical coherence tomography (OCT) could detect intraretinal changes during inflammation and to determine its utility as a tool for accurate scoring of EAU. EAU was induced in C57BL/6J mice and animals evaluated after 15, 26, 36 and 60 days. At each time-point, contemporaneous Spectralis-OCT scanning, topical endoscopic fundal imaging (TEFI), fundus fluorescein angiography (FFA) and CD45-immunolabelled histology were performed. OCT features were further characterised on retinal flat-mounts using immunohistochemistry and 3D reconstruction. Optic disc swelling and vitreous opacities detected by OCT corresponded to CD45+ cell infiltration on histology. Vasculitis identified by FFA and OCT matched perivascular myeloid and T-cell infiltrates and could be differentiated from unaffected vessels. Evolution of these changes could be followed over time in the same eye. Retinal folds were visible and found to encapsulate mixed populations of activated myeloid cells, T-cells and microglia. Using these features, an OCT-based EAU scoring system was developed, with significant correlation to validated histological (Pearson r(2) = 0.6392, P<0.0001, n = 31 eyes) and TEFI based scoring systems (r(2) = 0.6784, P<0.0001). OCT distinguishes the fundamental features of murine EAU in vivo, permits dynamic assessment of intraretinal changes and can be used to score disease severity. As a result, it allows tissue synchronisation with subsequent cellular and functional assessment and greater efficiency of animal usage. By relating OCT signals with immunohistochemistry in EAU, our findings offer the opportunity to inform the interpretation of OCT changes in human uveitis.
authors.affiliation: Department of Genetics, UCL Institute of Ophthalmology, London, United Kingdom.
authors.forename: Colin J
authors.initials: CJ
authors.lastname: Chu
authors.forename: Philipp
authors.initials: P
authors.lastname: Herrmann
authors.forename: Livia S
authors.initials: LS
authors.lastname: Carvalho
authors.forename: Sidath E
authors.initials: SE
authors.lastname: Liyanage
authors.forename: James W B
authors.initials: JW
authors.lastname: Bainbridge
authors.forename: Robin R
authors.initials: RR
authors.lastname: Ali
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Dick
authors.forename: Ulrich F O
authors.initials: UF
authors.lastname: Luhmann
mesh_terms: D000818:Animals; D001327:Autoimmune Diseases / Q000175:diagnosis* / Q000473:pathology; D001808:Blood Vessels / Q000473:pathology; D002138:Calibration; D004195:Disease Models, Animal; D018450:Disease Progression; D005260:Female; D006801:Humans; D051379:Mice; D009898:Optic Disk / Q000098:blood supply / Q000473:pathology; D012173:Retinitis / Q000175:diagnosis* / Q000473:pathology; D041623:Tomography, Optical Coherence; D014605:Uveitis / Q000175:diagnosis* / Q000473:pathology