title: [Rationality of the processing methods of aconiti lateralis radix (Fuzi) based on chemical analysis].
abstract: In this study, we explored the rationality of processing methods and mechanism of Aconiti Lateralis Radix (Fuzi) through comparing the chemical contents of diester alkaloids (DAs) and monoester alkaloids (MAs) in the raw material of Fuzi and its processed products. The results showed that the toxicity potency of MAs is at least lower than 1/64 to 1/180 of the toxicity potency of DAs. The contents of DAs in processed Fuzi decreased to 1/76.5 to 1/38.3 of the value of raw Fuzi. The contents of MAs in processed Fuzi significantly increased by 4.6 to 5.2 fold or basically the same as that of the raw Fuzi. The values of MAs/DAs of processed Fuzi were enhanced by 30 to 390 fold of the raw Fuzi. It was found that the contents of DAs were insignificantly different between "Wu dan fu pian" (steaming or stir-frying without Danba) and "Dan fu pian" (steaming or stir-frying with Danba). The result suggested that the abilities of "eliminating toxicity" of different processing methods were equivalent at all. In contrast, the contents of MAs contained in "Wu dan fu pian" were of 5.3 to 8.7 fold higher than the values in "Dan fu pian". This result suggested the processing method by steaming or stir-frying without Danba might have better effect for "conserving property" than the method processed with Danba stipulated by China Pharmacopoeia. We believe that the new processing method without Danba can be recommended in further application due to it offers a simple procedure and it will not introduce inorganic impurities in the products.
authors.affiliation: College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China.
authors.forename: Rui-Qing
authors.initials: RQ
authors.lastname: Wen
authors.forename: Dong-Hui
authors.initials: DH
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.forename: Jia-Bo
authors.initials: JB
authors.lastname: Wang
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Zhao
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Sun
authors.forename: Dan
authors.initials: D
authors.lastname: Yan
authors.forename: Xiao-He
authors.initials: XH
authors.lastname: Xiao
authors.forename: Yu-Zhen
authors.initials: YZ
authors.lastname: Ren
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Du
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Zhou
mesh_terms: D000157:Aconitine / Q000031:analogs & derivatives / Q000032:analysis* / Q000633:toxicity; D031841:Aconitum / Q000737:chemistry*; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D016000:Cluster Analysis; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000633:toxicity; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013678:Technology, Pharmaceutical / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; C479202:benzoylhypaconine; C047307:benzoylaconine; C058309:hypaconitine; C019470:mesaconitine; C085283:benzoylmesaconine; D000157:Aconitine