title: Skin autofluorescence relates to soluble receptor for advanced glycation end-products and albuminuria in diabetes mellitus.
abstract: The aim of this study was to compare skin autofluorescence caused by advanced glycation end-products (AGEs) with biochemical markers of endothelial dysfunction and soluble receptor for AGEs (sRAGE) in patients with diabetes. Skin autofluorescence (AF) assessed by AGE-Reader was evaluated with sRAGE and other biochemical parameters in 88 patients with diabetes (47 Type 1/T1DM/ and 41 Type 2/T2DM/) and 20 controls. Skin AF was significantly higher in T1DM and T2DM in comparison to controls (2.39 ± 0.54, 2.63 ± 0.73 versus 1.96 ± 0.33 AU; P < 0.0001). Positive correlation of AF with sRAGE was detected in T1DM and T2DM (r = 0.37, P < 0.02 and r = 0.60, P < 0.0001), but not in controls. Significantly higher AF values were found in patients with positive albuminuria as compared to those with normal albuminuria. Similarly, higher AF was detected in patients with endothelial dysfunction expressed by vWF, ICAM-1, and VCAM-1. Multiple regression analysis revealed independent association of skin AF with age, sRAGE, and albumin-creatinine ratio in patients with diabetes (R (2) = 0.38). Our study confirms that AF is elevated in patients with diabetes, especially with positive albuminuria and endothelial dysfunction. The strong and independent relationship between AF and sRAGE supports the idea that AF may reflect AGEs/RAGE interactions. The exact mechanism remains to be established.
authors.affiliation: Institute of Medical Biochemistry and Laboratory Diagnostics, First Faculty of Medicine, Charles University in Prague, General University Hospital in Prague, 128 08 Prague 2, Czech Republic ; 3rd Department of Internal Medicine, First Faculty of Medicine, Charles University in Prague, General University Hospital in Prague, 128 08 Prague 2, Czech Republic.
authors.forename: J
authors.initials: J
authors.lastname: Skrha
authors.forename: J
authors.initials: J
authors.lastname: Soupal
authors.forename: G
authors.initials: G
authors.lastname: Loni Ekali
authors.forename: M
authors.initials: M
authors.lastname: Prázný
authors.forename: M
authors.initials: M
authors.lastname: Kalousová
authors.forename: J
authors.initials: J
authors.lastname: Kvasnička
authors.forename: L
authors.initials: L
authors.lastname: Landová
authors.forename: T
authors.initials: T
authors.lastname: Zima
authors.forename: J
authors.initials: J
authors.lastname: Skrha