title: Preparation and application of reversed phase Chromatorotor for the isolation of natural products by centrifugal preparative chromatography.
abstract: A method of preparation of rotors with a reversed phase (RP) solid silica gel sorbent layer has been developed for centrifugal preparative chromatography (CPC), also known as rotational planar chromatography (RPC). The rotors consist of binder free RP solid SiO2 layers of different thicknesses packed between two supported circular glass discs and can be used in any appropriate device for centrifugal chromatography, like Chromatotron and CycloGraph. Polar and /or semi-polar compounds with close R(f) values, as well as extracts and column fractions were separated and/or purified in a preparative and/or semi-preparative scale using the RP rotors, eluted with mixtures of aqueous-based solvents. We herein report three examples of its application, using RP Chromatorotors, for the isolation of the diastereoisomeric alkaloids banistenosides I and II from Banisteriopsis caapi, saponins III and IV from Fagonia cretica, and the sesquiterpenes artemisinin (V) and artemisinic acid (VI) from Artemisia annua.
authors.affiliation: National Center for Natural Products Research, School of Pharmacy, Research Institute of Pharmaceutical Sciences, The University of Mississippi, University, MS 38677, USA. milias@olemiss.edu
authors.forename: Ilias
authors.initials: I
authors.lastname: Muhammad
authors.forename: Volodymyr
authors.initials: V
authors.lastname: Samoylenko
authors.forename: Francis
authors.initials: F
authors.lastname: Machumi
authors.forename: Mohamed Ahmed
authors.initials: MA
authors.lastname: Zakia
authors.forename: Rabab
authors.initials: R
authors.lastname: Mohammed
authors.forename: Mona H
authors.initials: MH
authors.lastname: Hetta
authors.forename: Van
authors.initials: V
authors.lastname: Gillum
mesh_terms: D031172:Artemisia annua / Q000737:chemistry; D037621:Artemisinins / Q000032:analysis; D001688:Biological Products / Q000032:analysis*; D002845:Chromatography / Q000379:methods*; D015394:Molecular Structure; D012503:Saponins / Q000032:analysis
chemical_list: D037621:Artemisinins; D001688:Biological Products; D012503:Saponins; C047721:artemisic acid