title: [Genetic diversity and genetic structure of endangered wild Sinopodophyllum emodi by start codon targeted polymorphism].
abstract: OBJECTIVE
  Revealed the genetic diversity level and genetic structure characteristics in Sinopodophyllum emodi, a rare and endangered species in China.
  
  
  METHODS
  We detected the genetic polymorphism within and among six wild populations (45 individuals) by the approach of Start Codon Targeted (SCoT) Polymorphism. The associated genetic parameters were calculated by POP-GENE1.31 and the relationship was constructed based on UPGMA method.
  
  
  RESULTS
  A total of 350 bands were scored by 27 primers and 284 bands of them were polymorphic. The average polymorphic bands of each primer were 10.52. At species level, there was a high level of genetic diversity among six populations (PPB = 79.27%, N(e) = 1.332 7, H = 0.210 9 and H(sp) = 0.328 6). At population level, the genetic diversity level was low (PPB = 10.48% (4.00% -23.71%), N(e) = 1.048 7 (1.020 7-1.103 7), H = 0.029 7 (0.012 9-0.063 1), H(pop) = 0.046 2 (0.019 9-0.098 6). The Nei's coefficient of genetic differentiation was 0.841 1, which was consistent with the Shannon's coefficient of genetic differentiation (0.849 4). Two calculated methods all showed that most of the genetic variation existed among populations. The gene flow (N(m) = 0.094 4) was less among populations, indicating that the degree of genetic differentiation was higher. Genetic similarity coefficient were changed from 0.570 8 to 0.978 7. By clustering analysis, the tested populations were divided into two classes and had a tendency that the same geographical origin or material of similar habitats clustered into one group.
  
  
  CONCLUSIONS
  The genetic diversity of samples of S. emodi is high,which laid a certain foundation for effective protection and improvement of germplasm resources.
authors.affiliation: Chongqing Academy of Chinese Materia Medica, National Engineering Laboratory for Propagation of Endangered Traditional Chinese Medicine, Chongqing 400065, China.
authors.forename: Da-Xia
authors.initials: DX
authors.lastname: Chen
authors.forename: Ji-Feng
authors.initials: JF
authors.lastname: Zhao
authors.forename: Xiang
authors.initials: X
authors.lastname: Liu
authors.forename: Chang-Hua
authors.initials: CH
authors.lastname: Wang
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Zhang
authors.forename: Song-Yun
authors.initials: SY
authors.lastname: Qin
authors.forename: Guo-Yue
authors.initials: GY
authors.lastname: Zhong
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D028141:Berberidaceae / Q000145:classification / Q000235:genetics*; D002681:China; D016000:Cluster Analysis; D018387:Codon, Initiator; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D056727:Endangered Species; D051456:Gene Flow; D005819:Genetic Markers / Q000235:genetics; D040342:Genetic Structures; D014644:Genetic Variation; D005843:Geography; D008516:Medicine, Chinese Traditional; D010802:Phylogeny; D010946:Plants, Medicinal; D011110:Polymorphism, Genetic; D012984:Software
chemical_list: D018387:Codon, Initiator; D017931:DNA Primers; D018744:DNA, Plant; D005819:Genetic Markers