title: Separation of phenylpropanoid glycosides from a Chinese herb by HSCCC.
abstract: An effective high-speed counter-current chromatography method was established for the preparative isolation and purification of two phenylpropanoid glycosides from the Tibetan medicinal plant Pedicularis longiflora Rudolph. var. tubiformis (Klotz). Tsoong. With a two-phase solvent system composed of chloroform-n-butanol-methanol-water (4:3:4:5, v/v), 40 mg of an extract of Pedicularis longiflora Rudolph. var. tubiformis (Klotz). Tsoong was separated to yield 20 mg of verbascoside and 18 mg of isoacteoside, with purity values of 97 and 98%, respectively. The chemical structures of these two components were identified by proton and carbon nuclear magnetic resonance. In addition, the antioxidant activity of the two phenylpropanoid glycosides was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH)-high-performance liquid chromatography, and the results showed that the two components exhibited strong antioxidant DPPH radical scavenging activity, with IC50 values of 15.6 and 18.9 µg/mL, respectively.
authors.affiliation: 1Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining 810001, P. R. China.
authors.forename: Chen
authors.initials: C
authors.lastname: Chen
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Zhao
authors.forename: Hui-Lan
authors.initials: HL
authors.lastname: Yue
authors.forename: Yu-Lin
authors.initials: YL
authors.lastname: Li
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
mesh_terms: D001713:Biphenyl Compounds / Q000737:chemistry; D003377:Countercurrent Distribution; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D016166:Free Radical Scavengers / Q000737:chemistry / Q000302:isolation & purification; D005609:Free Radicals / Q000737:chemistry; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification*; D038861:Medicine, Tibetan Traditional; D032262:Pedicularis / Q000737:chemistry*; D010851:Picrates / Q000737:chemistry
chemical_list: D001713:Biphenyl Compounds; D004365:Drugs, Chinese Herbal; D016166:Free Radical Scavengers; D005609:Free Radicals; D006027:Glycosides; D010851:Picrates; C004931:1,1-diphenyl-2-picrylhydrazyl