title: A mutation in the receptor Methoprene-tolerant alters juvenile hormone response in insects and crustaceans.
abstract: Juvenile hormone is an essential regulator of major developmental and life history events in arthropods. Most of the insects use juvenile hormone III as the innate juvenile hormone ligand. By contrast, crustaceans use methyl farnesoate. Despite this difference that is tied to their deep evolutionary divergence, the process of this ligand transition is unknown. Here we show that a single amino-acid substitution in the receptor Methoprene-tolerant has an important role during evolution of the arthropod juvenile hormone pathway. Microcrustacea Daphnia pulex and D. magna share a juvenile hormone signal transduction pathway with insects, involving Methoprene-tolerant and steroid receptor coactivator proteins that form a heterodimer in response to various juvenoids. Juvenile hormone-binding pockets of the orthologous genes differ by only two amino acids, yet a single substitution within Daphnia Met enhances the receptor's responsiveness to juvenile hormone III. These results indicate that this mutation within an ancestral insect lineage contributed to the evolution of a juvenile hormone III receptor system.
authors.affiliation: Okazaki Institute for Integrative Bioscience, National Institute for Basic Biology, National Institutes of Natural Sciences, Higashiyama, Myodaiji, Okazaki 444-8787, Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Miyakawa
authors.forename: Kenji
authors.initials: K
authors.lastname: Toyota
authors.forename: Ikumi
authors.initials: I
authors.lastname: Hirakawa
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Ogino
authors.forename: Shinichi
authors.initials: S
authors.lastname: Miyagawa
authors.forename: Shigeto
authors.initials: S
authors.lastname: Oda
authors.forename: Norihisa
authors.initials: N
authors.lastname: Tatarazako
authors.forename: Toru
authors.initials: T
authors.lastname: Miura
authors.forename: John K
authors.initials: JK
authors.lastname: Colbourne
authors.forename: Taisen
authors.initials: T
authors.lastname: Iguchi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D003621:Daphnia / Q000187:drug effects / Q000235:genetics*; D055785:Gene Knockdown Techniques; D007313:Insecta / Q000187:drug effects / Q000235:genetics*; D007605:Juvenile Hormones / Q000494:pharmacology*; D008024:Ligands; D008297:Male; D008726:Methoprene / Q000494:pharmacology*; D008969:Molecular Sequence Data; D050505:Mutant Proteins / Q000378:metabolism; D009154:Mutation / Q000235:genetics*; D010641:Phenotype; D055503:Protein Multimerization / Q000187:drug effects; D034622:RNA Interference; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000235:genetics*; D011987:Receptors, Steroid / Q000737:chemistry / Q000378:metabolism; D015533:Transcriptional Activation / Q000187:drug effects / Q000235:genetics
chemical_list: D007605:Juvenile Hormones; D008024:Ligands; D050505:Mutant Proteins; D011956:Receptors, Cell Surface; D011987:Receptors, Steroid; D008726:Methoprene