title: Lipogels: surface-adherent composite hydrogels assembled from poly(vinyl alcohol) and liposomes.
abstract: Drug-eluting engineered surface coatings are of paramount importance for many biomedical applications from implantable devices to tissue engineering. Herein, we present the assembly of lipogels, composite physical hydrogels assembled from poly(vinyl alcohol) and liposomes using thiol-disulfide exchange between end group modified PVA and thiocholesterol containing liposomes, and the response of adhering cells to these coatings. We demonstrate the controlled loading of liposomes into the polymer matrix and the preserved mechanical properties of the lipogels. Furthermore, the lipogels are successfully rendered cell adhesive by incorporation of poly(l-lysine) into the PVA polymer matrix or by poly(dopamine) coating of the lipogels. The successful lipid uptake from the lipogels by macrophages, hepatocytes, and myoblasts was monitored by flow cytometry. Finally, the delivery of active cargo, paclitaxel, to adherent myoblasts is shown, thus illustrating the potential of the lipogels as a drug eluting interface for biomedical applications.
authors.affiliation: Department of Chemistry, Aarhus University, Aarhus, Denmark.
authors.forename: Bettina E B
authors.initials: BE
authors.lastname: Jensen
authors.forename: Leticia
authors.initials: L
authors.lastname: Hosta-Rigau
authors.forename: Philipp R
authors.initials: PR
authors.lastname: Spycher
authors.forename: Erik
authors.initials: E
authors.lastname: Reimhult
authors.forename: Brigitte
authors.initials: B
authors.lastname: Städler
authors.forename: Alexander N
authors.initials: AN
authors.lastname: Zelikin
mesh_terms: D000818:Animals; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D020100:Hydrogels / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D007211:Indoles / Q000138:chemical synthesis / Q000494:pharmacology; D008081:Liposomes / Q000737:chemistry / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D032446:Myoblasts / Q000166:cytology / Q000378:metabolism*; D011107:Polylysine / Q000737:chemistry / Q000494:pharmacology; D011108:Polymers / Q000138:chemical synthesis / Q000494:pharmacology; D011142:Polyvinyl Alcohol / Q000737:chemistry / Q000494:pharmacology
chemical_list: D020100:Hydrogels; D007211:Indoles; D008081:Liposomes; D011108:Polymers; C568283:polydopamine; D011107:Polylysine; D011142:Polyvinyl Alcohol