title: A classical brown adipose tissue mRNA signature partly overlaps with brite in the supraclavicular region of adult humans.
abstract: Human brown adipose tissue (BAT) has been detected in adults but was recently suggested to be of brite/beige origin. We collected BAT from the supraclavicular region in 21 patients undergoing surgery for suspected cancer in the neck area and assessed the gene expression of established murine markers for brown, brite/beige, and white adipocytes. We demonstrate that a classical brown expression signature, including upregulation of miR-206, miR-133b, LHX8, and ZIC1 and downregulation of HOXC8 and HOXC9, coexists with an upregulation of two newly established brite/beige markers, TBX1 and TMEM26. A similar mRNA expression profile was observed when comparing isolated human adipocytes from BAT and white adipose tissue (WAT) depots, differentiated in vitro. In conclusion, our data suggest that human BAT might consist of both classical brown and recruitable brite adipocytes, an observation important for future considerations on how to induce human BAT.
authors.affiliation: Centre of Inflammation and Metabolism, Department of Infectious Diseases, Rigshospitalet, DK-2100 Copenhagen, Denmark.
authors.forename: Naja Zenius
authors.initials: NZ
authors.lastname: Jespersen
authors.forename: Therese Juhlin
authors.initials: TJ
authors.lastname: Larsen
authors.forename: Lone
authors.initials: L
authors.lastname: Peijs
authors.forename: Søren
authors.initials: S
authors.lastname: Daugaard
authors.forename: Preben
authors.initials: P
authors.lastname: Homøe
authors.forename: Annika
authors.initials: A
authors.lastname: Loft
authors.forename: Jasper
authors.initials: J
authors.lastname: de Jong
authors.forename: Neha
authors.initials: N
authors.lastname: Mathur
authors.forename: Barbara
authors.initials: B
authors.lastname: Cannon
authors.forename: Jan
authors.initials: J
authors.lastname: Nedergaard
authors.forename: Bente Klarlund
authors.initials: BK
authors.lastname: Pedersen
authors.forename: Kirsten
authors.initials: K
authors.lastname: Møller
authors.forename: Camilla
authors.initials: C
authors.lastname: Scheele
mesh_terms: D017667:Adipocytes / Q000378:metabolism; D052438:Adipocytes, White / Q000378:metabolism; D002001:Adipose Tissue, Brown / Q000378:metabolism*; D052436:Adipose Tissue, White / Q000378:metabolism; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics; D015536:Down-Regulation; D005260:Female; D006801:Humans; D007473:Ion Channels / Q000235:genetics / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D059467:Transcriptome; D000071256:Uncoupling Protein 1; D015854:Up-Regulation; D055815:Young Adult
chemical_list: D015415:Biomarkers; D007473:Ion Channels; D024101:Mitochondrial Proteins; D012333:RNA, Messenger; D000071256:Uncoupling Protein 1; D009638:Norepinephrine