title: [Impact of mass spectrometry by MALDI-TOF MS for the rapid identification of aerobic and anaerobic bacteria of clinical importance].
abstract: BACKGROUND
  MALDI-TOF MS (Matrix Assisted Laser Desorption Ionization -Time of Flight Mass Spectrometry) technology, recently introduced in the microbiology laboratory has proven to be a precise and rapid method for bacterial identification.
  
  
  OBJECTIVE
  To evaluate the performance, costs associated and turnaround time of MALDI-TOF in a routine laboratory.
  
  
  METHODS
  Five hundred and sixty one clinical isolates (281 aerobes and 280 anaerobes) previously identified by conventional methods were evaluated. Discordances were resolved by means of 16S rRNA sequencing.
  
  
  RESULTS
  MALDI-TOF identified 95, 7% of the aerobes isolates and 86, 4% of the anaerobes. The groups with better performance were the enterobacteriacea and Bacteroides spp with 95% and 100% identification at the species level. The error rate of MALDI-TOF and conventional methods compared to sequencing was 0, 39% and 9, 4% respectively. The costs associated were 8 times lower with a turnaround time of 6 hours.
  
  
  CONCLUSIONS
  MALDI-TOF proved to be simple, precise and less expensive technology compared to the traditional methods.
authors.affiliation: Departamento de Laboratorios Clínicos, Escuela de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile.
authors.forename: Paulette
authors.initials: P
authors.lastname: Legarraga
authors.forename: Marcela
authors.initials: M
authors.lastname: Moraga
authors.forename: Marusella
authors.initials: M
authors.lastname: Lam
authors.forename: Enrique
authors.initials: E
authors.lastname: Geoffroy
authors.forename: Cecilia
authors.initials: C
authors.lastname: Zumarán
authors.forename: Patricia
authors.initials: P
authors.lastname: García
mesh_terms: D001420:Bacteria, Aerobic / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001421:Bacteria, Anaerobic / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques / Q000379:methods*; D003365:Costs and Cost Analysis; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D006801:Humans; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S