title: Novel interactions of caffeic acid with different hemoglobins: effects on discoloration and lipid oxidation in different washed muscles.
abstract: Caffeic acid (CA) accelerated methemoglobin (Hb) formation at pH5.8 and 25°C. This was attributed to electron donation from CA to liganded O2 in Hb. CA inhibited hemin dissociation from metHb. Pig Hb remained mostly as oxyHb and did not promote lipid oxidation in washed cod muscle (WCM) nor washed turkey muscle (WTM) during iced storage at pH5.8. Conversely, perch Hb rapidly was converted to metHb and readily promoted lipid oxidation based on lipid peroxide and hexanal formation. CA strongly inhibited perch Hb-mediated lipid oxidation during storage. Once metHb formation occurred in WCM, CA appeared to maintain the heme protein as metHb during the remainder of iced storage. CA may have become bound to perch Hb based on filtration analysis. CA facilitated the transfer of perch Hb (but not pig Hb) from the aqueous phase to the insoluble components of WCM. Collectively, these results suggest that CA inhibited Hb-mediated lipid oxidation by various mechanisms not related to inhibition of metHb formation.
authors.affiliation: Meat Science and Muscle Biology Laboratory, Department of Animal Science, University of Wisconsin-Madison, Madison, WI 53706-1284, USA.
authors.forename: Sung Yong
authors.initials: SY
authors.lastname: Park
authors.forename: Ingrid
authors.initials: I
authors.lastname: Undeland
authors.forename: Thippeswamy
authors.initials: T
authors.lastname: Sannaveerappa
authors.forename: Mark P
authors.initials: MP
authors.lastname: Richards
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002109:Caffeic Acids / Q000737:chemistry / Q000494:pharmacology*; D003116:Color; D004591:Electrophoresis, Polyacrylamide Gel; D006427:Hemin / Q000037:antagonists & inhibitors / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D050356:Lipid Metabolism / Q000187:drug effects*; D008054:Lipid Peroxides / Q000378:metabolism; D008706:Methemoglobin / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000737:chemistry*; D010471:Perches; D013552:Swine; D014422:Turkeys
chemical_list: D000975:Antioxidants; D002109:Caffeic Acids; D008054:Lipid Peroxides; D006427:Hemin; D008706:Methemoglobin; C040048:caffeic acid