title: Flush at room temperature followed by storage on ice creates the best lung graft preservation in rats.
abstract: Current clinical lung preservation techniques have not eliminated ischaemia-reperfusion (I/R) injury, despite many improvements. The optimal combination of flush and storage temperatures remain unclear in lung preservation. This is the first study to investigate a range of temperatures with 24-h inflated storage using consistent state-of-the-art preservation techniques. A rat lung transplant model was used to investigate the optimal combination of flush and storage temperatures. In six groups, rat lungs were flushed at 4 °C, 10 °C or room temperature (F(4) /F(10) /F(Rt)) with Perfadex and stored inflated for 24 h in Perfadex on melting ice or at 10 °C (S(ice) /S(10)). Left donor lungs were transplanted for analysis. During 2-h reperfusion, the lung graft function was measured (blood gases, maximum ventilation pressure and static compliance) and lung graft injury was also assessed (W/D ratio, total lung protein, Tryptase, Myeloperoxidase). Right donor lungs were assessed for W/D ratio only after flush and storage. For baseline measurements, left lungs without intervention were used. The combination of F(Rt) -S(ice) showed a significantly higher pO(2), lower P(max), low W/D ratios and total protein levels of left lungs after reperfusion when compared with F(4) -S(ice) and baseline. Storage at 10 °C did not improve preservation. We conclude that F(Rt) -S(ice) creates the best lung graft preservation.
authors.affiliation: Department of Biomedical Engineering, UMCG, University of Groningen, Groningen, The Netherlands. aj.munneke@umcg.nl
authors.forename: Anita J
authors.initials: AJ
authors.lastname: Munneke
authors.forename: Gerhard
authors.initials: G
authors.lastname: Rakhorst
authors.forename: Arjen H
authors.initials: AH
authors.lastname: Petersen
authors.forename: Wim
authors.initials: W
authors.lastname: van Oeveren
authors.forename: Jochum
authors.initials: J
authors.lastname: Prop
authors.forename: Michiel E
authors.initials: ME
authors.lastname: Erasmus
mesh_terms: D000818:Animals; D016040:Lung Transplantation / Q000379:methods*; D009926:Organ Preservation / Q000379:methods*; D051381:Rats; D011917:Rats, Inbred Lew; D015424:Reperfusion; D013696:Temperature; D053802:Tryptases / Q000097:blood
chemical_list: D053802:Tryptases