title: 8-Aminoquinoline-based ratiometric zinc probe: unexpected binding mode and its application in living cells.
abstract: PMQA, an 8-aminoquinoline-based ratiometric fluorescent sensor, demonstrates the Zn(2+)-induced red-shift of emission (85nm), and was successfully applied to image zinc in living cells. Compared to 2:1 stoichiometry in PMQA-Zn(2+), PMQA-Cu(2+) shows 1:1 composition. Both nitrogen atoms from the aminoquinoline are missing in binding of zinc, while they are critically involved in Cu(2+) chelation. The structure difference between PMQA-Zn(2+) and PMQA-Cu(2+) might shed light in designing novel zinc probes without suffering from copper interference.
authors.affiliation: State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, PR China.
authors.forename: Liangwei
authors.initials: L
authors.lastname: Zhang
authors.forename: Xuemei
authors.initials: X
authors.lastname: Cui
authors.forename: Jinyu
authors.initials: J
authors.lastname: Sun
authors.forename: Yuli
authors.initials: Y
authors.lastname: Wang
authors.forename: Weishuang
authors.initials: W
authors.lastname: Li
authors.forename: Jianguo
authors.initials: J
authors.lastname: Fang
mesh_terms: D000634:Aminoquinolines / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D002614:Chelating Agents / Q000737:chemistry; D056831:Coordination Complexes / Q000737:chemistry*; D003300:Copper / Q000737:chemistry; D018360:Crystallography, X-Ray; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D013050:Spectrometry, Fluorescence / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D015032:Zinc / Q000032:analysis* / Q000737:chemistry
chemical_list: D000634:Aminoquinolines; D002614:Chelating Agents; D056831:Coordination Complexes; D005456:Fluorescent Dyes; D003300:Copper; D015032:Zinc; C080436:8-aminoquinoline