title: A new method of establishing orthotopic bladder transplantable tumor in mice.
abstract: OBJECTIVE
  The present study aims to find a convenient, rapid, and stable method to establish bladder tumor in mice.
  
  
  METHODS
  Female Balb/C-nu-nu nude mice (or female T739 mice) were narcotized by sodium pentobarbital at a dosage of 60 mg/kg. The stylet of the 24# venous retention needles was bent in a 5° to 7° angle at a distance of 15 mm from the needlepoint to form a circle with 2.61 mm to 3.66 mm radius when the stylet is rotated. The pipe casing was lubricated with liquid paraffin, and inserted into the bladder cavity. The drift angle stylet was inserted into the pipe casing slowly, rotated for five times, and then pulled out. A cell suspension (0.1 mL) of approximately 1×10(6) T24 cells (or BTT cells) was then injected immediately.
  
  
  RESULTS
  A total of 60 T739 mice and 60 Balb/C-nu-nu nude mice were inoculated with BTT cells and T24 cells, respectively. The bladder tumor incidence and the average survival time of the tumor-bearing mice were 100% and (26.69±9.24) d and 100% and (34.59±9.8) d for the T739 mice and Balb/C-nu-nu nude mice, respectively.
  
  
  CONCLUSIONS
  Using the drift angle stylet to injure the mucous membrane of the urinary bladder can establish a stable bladder transplantable tumor model in mice.
authors.affiliation: Department of Comparative Medicine, Shanxi Cancer Institute, Taiyuan 030013, China.
authors.forename: Xi-Hua
authors.initials: XH
authors.lastname: Yang
authors.forename: Lian-Sheng
authors.initials: LS
authors.lastname: Ren
authors.forename: Guo-Ping
authors.initials: GP
authors.lastname: Wang
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Zhao
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Zhen-Guo
authors.initials: ZG
authors.lastname: Mi
authors.forename: Xihua
authors.initials: X
authors.lastname: Bai
keywords: bladder tumor; mice; model