title: A convenient and efficient purification method for chemically labeled oligonucleotides.
abstract: We developed an efficient, cost-effective, and rapid purification method for chemically-labeled oligonucleotides that requires less time than conventional procedures such as ethanol precipitation or size-exclusion chromatography. Based on the hydrophilic and hydrophobic properties of DNA and amine-reactive fluorophores, we show that n-butanol saturated with distilled water may be used to remove unreacted fluorophores by sequestering them in the organic phase, while labeled DNA remains in the aqueous phase. This phase extraction method is simple, fast, and allows for processing multiple samples simultaneously, a necessity for high-throughput labeling strategies.
authors.affiliation: WCU Department of Biophysics and Chemical Biology, Seoul National University, Korea.
authors.forename: Jihee
authors.initials: J
authors.lastname: Hwang
authors.forename: Junhee
authors.initials: J
authors.lastname: Kang
authors.forename: Seong Keun
authors.initials: SK
authors.lastname: Kim
authors.forename: Younggyu
authors.initials: Y
authors.lastname: Kim
mesh_terms: D020001:1-Butanol / Q000737:chemistry; D000097:Acetonitriles / Q000737:chemistry; D002232:Carbocyanines / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry / Q000302:isolation & purification*; D059625:Liquid-Liquid Extraction / Q000379:methods*; D009841:Oligonucleotides / Q000737:chemistry / Q000302:isolation & purification*; D013050:Spectrometry, Fluorescence
chemical_list: D000097:Acetonitriles; D002232:Carbocyanines; D005456:Fluorescent Dyes; D009841:Oligonucleotides; C085321:cyanine dye 5; D020001:1-Butanol; C032159:acetonitrile