title: Indigenous plasmids of Bacillus megaterium WSH-002 involved in mutualism with Ketogulonicigenium vulgare WSH-001.
abstract: In the two-step vitamin C fermentation process, the precursor 2-keto-l-gulonic acid (2-KLG) was synthesized using a mixed culture of Ketogulonicigenium vulgare WSH-001 and Bacillus megaterium WSH-002, which contained three plasmids, pBME1, pBME2 and pBME3. The cell growth of B. megaterium was not affected by the elimination of these plasmids. However, elimination of pBME2 and pBME3 significantly affected l-sorbose uptake and 2-KLG production. Sequence analysis of the plasmids showed that many of the pBME2 and pBME3 genes were of unknown function or could not be assigned to a specific metabolic pathway. The current work showed that the indigenous plasmids pBME2 and pBME3 of B. megaterium WSH-002 involved in mutualism with K. vulgare WSH-001. The results provided a promising new route to further demonstrate the mutualism process between the two bacteria.
authors.affiliation: School of Biotechnology and Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China.
authors.forename: Jingwen
authors.initials: J
authors.lastname: Zhou
authors.forename: Qiaoshuang
authors.initials: Q
authors.lastname: Zheng
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Guocheng
authors.initials: G
authors.lastname: Du
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
mesh_terms: D020561:Alphaproteobacteria / Q000235:genetics*; D001410:Bacillus megaterium / Q000235:genetics* / Q000254:growth & development*; D001483:Base Sequence; D019295:Computational Biology; D017931:DNA Primers / Q000235:genetics; D005798:Genes, Bacterial / Q000235:genetics*; D058977:Molecular Sequence Annotation; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013400:Sugar Acids / Q000378:metabolism; D013559:Symbiosis / Q000235:genetics*
chemical_list: D017931:DNA Primers; D013400:Sugar Acids; C011491:provitamin C
keywords: 2-Keto-l-gulonic acid; Plasmid elimination; Symbiosis; Vitamin C; l-ascorbic acid