title: Oxidative stress markers in intestinal mucosa of Tunisian inflammatory bowel disease patients.
abstract: BACKGROUND / AIMS: Inflammatory bowel diseases (IBDs), Crohn's disease (CrD) and ulcerative colitis (UC), are chronic gastrointestinal inflammatory disorders. The precise etiology of IBD remains unclear, and it is thought that interactions among various factors, including, genetic factors, the host immune system and environmental factors, cause disruption of intestinal homeostasis, leading to dysregulated inflammatory responses of the gut. As inflammation is intimately related to formation of reactive intermediates, including, reactive oxygen species, oxidative stress has been proposed as a mechanism underlying the pathophysiology of IBD. The purpose of this study is to examine the lipid peroxidation, protein oxidation and anti-oxidative profile in Tunisian IBD.
  
  
  METHODS
  Malondialdehyde (MDA), conjugated dienes (CD), protein thiol levels, as well as the catalase (CAT) activity were evaluated in intestinal biopsies of 17 patients affected by IBD (12 CrD and 5 UC) and 12 healthy control individuals.
  
  
  RESULTS
  Oxidative stress was confirmed in these two types of disease biopsies as compared to controls. MDA and CD levels were significantly increased in both UC and CrD patients' biopsies as compared to controls' biopsies ( P < 0.001). CAT activity was similar in UC and CrD biopsies' and was not significantly increased in IBD patients' biopsies compared with controls' biopsies ( P > 0.05). Anon-significant decrease in thiol (SH) level was observed in both UC and CrD patients' biopsies compared with controls' biopsies ( P > 0.05).
  
  
  CONCLUSIONS
  Increased levels of MDA and CD in IBD patients' biopsies underline the implication of oxidative stress in the physiopathology of IBD.
authors.affiliation: Department of Immunology, Medicine School and Habib Bourguiba Hospital, Sfax, Tunisia. dorra-ing@voila.fr
authors.forename: Dorra
authors.initials: D
authors.lastname: Bouzid
authors.forename: Bochra
authors.initials: B
authors.lastname: Gargouri
authors.forename: Riadh Ben
authors.initials: RB
authors.lastname: Mansour
authors.forename: Ali
authors.initials: A
authors.lastname: Amouri
authors.forename: Nabil
authors.initials: N
authors.lastname: Tahri
authors.forename: Saloua
authors.initials: S
authors.lastname: Lassoued
authors.forename: Hatem
authors.initials: H
authors.lastname: Masmoudi
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D002374:Catalase / Q000378:metabolism; D005260:Female; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D007413:Intestinal Mucosa / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000502:physiology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology*; D013438:Sulfhydryl Compounds / Q000378:metabolism; D014416:Tunisia; D055815:Young Adult
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D013438:Sulfhydryl Compounds; D008315:Malondialdehyde; D002374:Catalase