title: Poliovirus: generation and characterization of mutants.
abstract: Poliovirus (PV) is the prototypical picornavirus. It is a non-enveloped RNA virus with a small (~7.5-kb) genome of positive polarity. cDNA clones of several strains are available, and infectious virus can be produced by the transfection of in vitro-transcribed viral genomes into an appropriate host cell. The ease of genetic studies in poliovirus is a primary reason that it has long served as a model to study RNA virus biology, pathogenesis, and evolution. Protocols for the generation and characterization of PV mutants are presented.
authors.affiliation: Department of Microbiology and Immunology, University of California, San Francisco, California.
authors.forename: Cecily P
authors.initials: CP
authors.lastname: Burrill
authors.affiliation: Department of Microbiology and Immunology, University of California, San Francisco, California.
authors.forename: Vanessa R
authors.initials: VR
authors.lastname: Strings
authors.affiliation: Department of Microbiology and Immunology, University of California, San Francisco, California.
authors.forename: Michael B
authors.initials: MB
authors.lastname: Schulte
authors.affiliation: Department of Microbiology and Immunology, University of California, San Francisco, California.
authors.forename: Raul
authors.initials: R
authors.lastname: Andino
mesh_terms: D005827:Genetics, Microbial / Q000379:methods*; D009154:Mutation; D017955:Poliovirus / Q000235:genetics* / Q000302:isolation & purification*; D014773:Virology / Q000379:methods*