title: Molecular epidemiology of β-lactamase-producing Neisseria gonorrhoeae strains in Manaus, AM, Brazil.
abstract: We report new sequence types of 14 penicillinase-producing Neisseria gonorrhoeae, isolated from sexually transmitted disease clinic attendees in Manaus, Brazil. They were characterized by WI/WII/WIII groups, susceptibility testing and Multi-Antigen Sequencing Typing/Mutilocus Sequence Typing protocols. Twelve were classified as WII/III and 2 as WI and were presented resistance to penicillin and tetracycline. New alleles for por and AroE genes and novel sequence types were identified, revealing molecular characteristics not described previously. ST1590 is the common ancestor after eBURST analysis, and these findings represent an important contribution of molecular epidemiology approach in gonococci's research in Amazonas.
authors.affiliation: Tropical and Infectious Disease, Universidade do Estado do Amazonas, Fundação Alfredo da Matta-FUAM, Laboratório de Bacteriologia, Clínica Rua Codajás, Manaus, Amazonas, Brazil. wianfe@yahoo.com.br
authors.forename: William Antunes
authors.initials: WA
authors.lastname: Ferreira
authors.forename: Cristina Motta
authors.initials: CM
authors.lastname: Ferreira
authors.forename: Felipe Gomes
authors.initials: FG
authors.lastname: Naveca
authors.forename: Waldemara
authors.initials: W
authors.lastname: de Souza Vasconcelos
authors.forename: Jairo
authors.initials: J
authors.lastname: de Souza Gomes
authors.forename: Maria de Fátima Pinto
authors.initials: Mde F
authors.lastname: da Silva
authors.forename: Maria das Graças Costa
authors.initials: Md
authors.lastname: Alecrim
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001938:Brazil / Q000453:epidemiology; D005260:Female; D005838:Genotype; D006069:Gonorrhea / Q000453:epidemiology* / Q000382:microbiology; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D017720:Molecular Epidemiology; D009344:Neisseria gonorrhoeae / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D010403:Penicillin Resistance; D012703:Serotyping; D013753:Tetracycline Resistance; D055815:Young Adult; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001618:beta-Lactamases