title: [Study on antioxidant interaction of different preparations and proportions of Danggui-Chuanxiong drug pair].
abstract: OBJECTIVE
  To observe the in vitro antioxidant interaction of different preparations and proportions of Danggui-Chuanxiong drug pair in the DPPH free radical scavenging rate with the response surface methodology.
  
  
  METHODS
  The 2,2-diphenyl-1-picryl-hydrazyl (DPPH) free radical scavenging rate method was adopted for determining the antioxidant activity of extracts from Danggui-Chuanxiong with 10 proportions and three extraction processes. The response surface methodology was used to determine the parameters of the dose-effect curve and establish a three-dimensional response surface model. The three-dimensional response surface graph was constructed with Matlab software.
  
  
  RESULTS
  All of the 30 samples with different proportions and preparations had antioxidant effect in scavenging free radicals and a remarkable dose-effect relationship. Their water extracts had a narrow synergistic range, with only spot distribution. Their antagonist ranges were districted in six bands of various widths. The synergistic ranges of ethanol extracts were districted in small bands, with the antagonist ranges scattered in points. The synergistic ranges of their water-alcohol extracts were distributed in three bands, with their antagonist ranges scattered in points. In short, the water-alcohol extracts showed a wider synergistic range than ethanol extracts, followed by water-extracts. All of the three extraction processes showed no obvious synergistic and antagonist effects.
  
  
  CONCLUSIONS
  The quantitative study on the interaction of traditional Chinese medicines with different compatibilities with the response surface methodology provides reference of thoughts and methods for relevant studies.
authors.affiliation: Jiangsu Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae, Nanjing University of Chinese Medicine, Nanjing 210046, China. maomilove321@163.com
authors.forename: Mei-Yan
authors.initials: MY
authors.lastname: Huang
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Tang
authors.forename: Er-Xin
authors.initials: EX
authors.lastname: Shang
authors.forename: Jian-Ming
authors.initials: JM
authors.lastname: Guo
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Lin-Yan
authors.initials: LY
authors.lastname: Wang
authors.forename: Wei-Xia
authors.initials: WX
authors.lastname: Li
authors.forename: Jin-Ao
authors.initials: JA
authors.lastname: Duan
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D001713:Biphenyl Compounds / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D004365:Drugs, Chinese Herbal / Q000378:metabolism*; D016166:Free Radical Scavengers / Q000378:metabolism; D008516:Medicine, Chinese Traditional; D010084:Oxidation-Reduction; D010851:Picrates / Q000378:metabolism
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D004365:Drugs, Chinese Herbal; D016166:Free Radical Scavengers; D010851:Picrates; C004931:1,1-diphenyl-2-picrylhydrazyl