title: Identification of gene microarray expression profiles in patients with chronic graft-versus-host disease following allogeneic hematopoietic cell transplantation.
abstract: Chronic graft-versus-host disease (GVHD) results in significant morbidity and mortality, limiting the benefit of allogeneic hematopoietic cell transplantation (HCT). Peripheral blood gene expression profiling of the donor immune repertoire following HCT may provide associated genes and pathways thereby improving the pathophysiologic understanding of chronic GVHD. We profiled 70 patients and identified candidate genes that provided mechanistic insight in the biologic pathways that underlie chronic GVHD. Our data revealed that the dominant gene signature in patients with chronic GVHD represented compensatory responses that control inflammation and included the interleukin-1 decoy receptor, IL-1 receptor type II, and genes that were profibrotic and associated with the IL-4, IL-6 and IL-10 signaling pathways. In addition, we identified three genes that were important regulators of extracellular matrix. Validation of this discovery phase study will determine if the identified genes have diagnostic, prognostic or therapeutic implications.
authors.affiliation: Department of Medicine Stanford University School of Medicine, 300 Pasteur Drive, Stanford, CA 94305 USA.
authors.forename: Holbrook E
authors.initials: HE
authors.lastname: Kohrt
authors.forename: Lu
authors.initials: L
authors.lastname: Tian
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Ash A
authors.initials: AA
authors.lastname: Alizadeh
authors.forename: Sue
authors.initials: S
authors.lastname: Hsieh
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Tibshirani
authors.forename: Samuel
authors.initials: S
authors.lastname: Strober
authors.forename: Minnie
authors.initials: M
authors.lastname: Sarwal
authors.forename: Robert
authors.initials: R
authors.lastname: Lowsky
mesh_terms: D000328:Adult; D015331:Cohort Studies; D003430:Cross-Sectional Studies; D005260:Female; D020869:Gene Expression Profiling; D006086:Graft vs Host Disease / Q000235:genetics* / Q000276:immunology; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D006801:Humans; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D011446:Prospective Studies; D012313:RNA / Q000737:chemistry / Q000235:genetics; D012189:Retrospective Studies; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult
chemical_list: D012313:RNA