title: A tyrosinase biosensor based on ordered mesoporous carbon-Au/L-lysine/Au nanoparticles for simultaneous determination of hydroquinone and catechol.
abstract: A novel biosensor was developed based on tyrosinase immobilization with ordered mesoporous carbon-Au (OMC-Au), L-lysine membrane and Au nanoparticles on a glassy carbon electrode (GCE). It was applied for the simultaneous determination of dihydroxybenzene isomers using differential pulse voltammetry (DPV). The tyrosinase/OMC-Au/L-lysine/Au film was characterized by scanning electron microscopy (SEM) and impedance spectra. Under optimized conditions, the DPV study results for two isomers, hydroquinone (HQ, 1,4-dihydroxybenzene) and catechol (CC, 1,2-dihydroxybenzene) showed low peak potentials, and the peak-to-peak difference was about 135.85 mV, which ensured the anti-interference ability of the biosensor and made simultaneous detection of dihydroxybenzene isomers possible in real samples. DPV peak currents increased linearly with concentration over the range of 4.0 × 10(-7) to 8.0 × 10(-5) M, and the detection limits of hydroquinone and catechol were 5 × 10(-8) M and 2.5 × 10(-8) M (S/N = 3), respectively. The tyrosinase biosensor exhibited good repeatability and stability. In addition, the response mechanism of enzyme catalysed redox on the OMC-Au/L-lysine/Au film modified electrode based on electrochemical study was discussed. The proposed method could be extended for the development of other enzyme-based biosensors.
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha, 410082, PR China. tanglin@hnu.edu.cn
authors.forename: Lin
authors.initials: L
authors.lastname: Tang
authors.forename: Yaoyu
authors.initials: Y
authors.lastname: Zhou
authors.forename: Guangming
authors.initials: G
authors.lastname: Zeng
authors.forename: Zhen
authors.initials: Z
authors.lastname: Li
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Liu
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Guiqiu
authors.initials: G
authors.lastname: Chen
authors.forename: Guide
authors.initials: G
authors.lastname: Yang
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Lei
authors.forename: Mengshi
authors.initials: M
authors.lastname: Wu
mesh_terms: D000363:Agaricales / Q000201:enzymology; D055162:Biocatalysis; D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry*; D002396:Catechols / Q000032:analysis* / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D006873:Hydroquinones / Q000032:analysis* / Q000737:chemistry; D007536:Isomerism; D007700:Kinetics; D008239:Lysine / Q000737:chemistry*; D008567:Membranes, Artificial; D053768:Metal Nanoparticles / Q000737:chemistry; D014442:Monophenol Monooxygenase / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D016062:Porosity; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D002396:Catechols; D004800:Enzymes, Immobilized; D006873:Hydroquinones; D008567:Membranes, Artificial; D014867:Water; D002244:Carbon; D006046:Gold; D014442:Monophenol Monooxygenase; D008239:Lysine; C034221:catechol; C031927:hydroquinone