title: Exploring neural cell dynamics with digital holographic microscopy.
abstract: In this review, we summarize how the new concept of digital optics applied to the field of holographic microscopy has allowed the development of a reliable and flexible digital holographic quantitative phase microscopy (DH-QPM) technique at the nanoscale particularly suitable for cell imaging. Particular emphasis is placed on the original biological information provided by the quantitative phase signal. We present the most relevant DH-QPM applications in the field of cell biology, including automated cell counts, recognition, classification, three-dimensional tracking, discrimination between physiological and pathophysiological states, and the study of cell membrane fluctuations at the nanoscale. In the last part, original results show how DH-QPM can address two important issues in the field of neurobiology, namely, multiple-site optical recording of neuronal activity and noninvasive visualization of dendritic spine dynamics resulting from a full digital holographic microscopy tomographic approach.
authors.affiliation: Centre de Neurosciences Psychiatriques, Centre Hospitalier Universitaire Vaudois (CHUV), Département de Psychiatrie, Site de Cery, CH-1008 Prilly/Lausanne, Switzerland.
authors.forename: P
authors.initials: P
authors.lastname: Marquet
authors.forename: C
authors.initials: C
authors.lastname: Depeursinge
authors.forename: P J
authors.initials: PJ
authors.lastname: Magistretti
mesh_terms: D000465:Algorithms; D000818:Animals; D003585:Cell Biology; D049229:Dendritic Spines / Q000473:pathology*; D004594:Electrophysiology / Q000379:methods; D004867:Equipment Design; D006696:Holography / Q000379:methods*; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D021621:Imaging, Three-Dimensional; D008853:Microscopy / Q000379:methods*; D009415:Nerve Net; D009474:Neurons / Q000473:pathology*; D055095:Optics and Photonics