title: Evaluation of the double-disk synergy test for New Delhi metallo-β-lactamase-1 and other metallo-β-lactamase producing gram-negative bacteria by using metal-ethylenediaminetetraacetic acid complexes.
abstract: New Delhi metallo-β-lactamase-1 (NDM-1), one of the metallo-β-lactamases (MBLs), has been identified from clinical isolates worldwide. Rapid detection of NDM-1 producers is necessary to prevent their dissemination. Seven types of EDTA complexes were evaluated as MBL inhibitors in double-disk synergy tests (DDSTs), resulting in detection of the first isolate of NDM-1-producing Escherichia coli (NDM-1 Dok01) in Japan. NDM-1 Dok01 was detected when EDTA magnesium disodium salt tetrahydrate (Mg-EDTA), EDTA calcium disodium salt dihydrate, EDTA cobalt disodium salt tetrahydrate and EDTA copper disodium salt tetrahydrate were used as MBL inhibitors. The sensitivity and specificity of DDSTs using Mg-EDTA for 75 MBL producers and 25 non-MBL producers were 96.0% and 100%, respectively. These findings indicate that the DDST method using Mg-EDTA can detect MBL-producing strains, including NDM-1 producers.
authors.affiliation: Biochemical Research Laboratories, Research and Development Division, Eiken Chemical, 143 Nogi, Nogi-machi Shimotsuga-gun, Tochigi 329-0114, Japan. momoko_fujisaki@eiken.co.jp
authors.forename: Momoko
authors.initials: M
authors.lastname: Fujisaki
authors.forename: Shinya
authors.initials: S
authors.lastname: Sadamoto
authors.forename: Akira
authors.initials: A
authors.lastname: Hishinuma
mesh_terms: D001431:Bacteriological Techniques / Q000379:methods*; D004492:Edetic Acid / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000378:metabolism; D006090:Gram-Negative Bacteria / Q000201:enzymology*; D012680:Sensitivity and Specificity; D065093:beta-Lactamase Inhibitors; D001618:beta-Lactamases / Q000032:analysis*
chemical_list: D004791:Enzyme Inhibitors; D065093:beta-Lactamase Inhibitors; D004492:Edetic Acid; D001618:beta-Lactamases; C552864:beta-lactamase NDM-1