title: Development and Validation of Spectrofluorimetric Method for Determination of Biotin in Bulk and Pharmaceutical Preparations via its Oxidation with Cerium (IV).
abstract: A simple and sensitive spectrofluorimetric method was developed for the determination of biotin in pure form and in pharmaceutical preparations. The proposed method is based on the oxidation of the drug with cerium (IV) ammonium sulfate in acidic medium. The fluorescence of the produced Cerium (III) was measured at 365 nm after excitation at 255 nm. The different experimental parameters affecting the development and stability of the reaction were carefully studied and optimized. The method is applicable over the concentration range of 30-120 ng/mL with correlation coefficient of 0.9998. The detection limit (LOD) of biotin was 2.41 ng/mL while quantitation limit (LOQ) was 7.29 ng/mL. The proposed procedure was successfully applied for the determination of biotin in pharmaceutical preparations with mean recoveries of 99.55 ± 0.83 and 101.67 ± 1.53 for biotin ampoules and capsules, respectively. The results obtained were in good agreement with those obtained using the official method.
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.
authors.forename: M I
authors.initials: MI
authors.lastname: Walash
authors.forename: M
authors.initials: M
authors.lastname: Rizk
authors.forename: Z A
authors.initials: ZA
authors.lastname: Sheribah
authors.forename: M M
authors.initials: MM
authors.lastname: Salim
keywords: biotin; cerium (IV); oxidation; pharmaceutical preparations; spectrofluorimetry