title: An evaluation of the anti-tumor efficacy of oleanolic acid-loaded PEGylated liposomes.
abstract: The effective delivery of oleanolic acid (OA) to the target site has several benefits in therapy for different pathologies. However, the delivery of OA is challenging due to its poor aqueous solubility. The study aims to evaluate the tumor inhibition effect of the PEGylated OA nanoliposome on the U14 cervical carcinoma cell line. In our previous study, OA was successfully encapsulated into PEGylated liposome with the modified ethanol injection method. Oral administration of PEGylated OA liposome was demonstrated to be more efficient in inhibiting xenograft tumors. The results of organ index indicated that PEG liposome exhibited higher anti-tumor activity and lower cytotoxicity. It was also found that OA and OA liposomes induced tumor cell apoptosis detected by flow cytometry. Furthermore, effects of OA on the morphology of tumor and other tissues were observed by hematoxylin and eosin staining. The histopathology sections did not show pathological changes in kidney or liver in tested mice. In contrast, there was a significant difference in tumor tissues between treatment groups and the negative control group. These observations imply that PEGylated liposomes seem to have advantages for cancer therapy in terms of effective delivery of OA.
authors.affiliation: Applied Chemical Key Lab of Hebei Province, College of Environmental and Chemical Engineering, Yanshan University, No. 438 Hebei Street, Qinhuangdao 066004, People's Republic of China.
authors.forename: Shengnan
authors.initials: S
authors.lastname: Tang
authors.forename: Dawei
authors.initials: D
authors.lastname: Gao
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhao
authors.forename: Jing
authors.initials: J
authors.lastname: Zhou
authors.forename: Xiaoning
authors.initials: X
authors.lastname: Zhao
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001835:Body Weight / Q000187:drug effects; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D006801:Humans; D007668:Kidney / Q000187:drug effects / Q000473:pathology; D008081:Liposomes / Q000737:chemistry* / Q000648:ultrastructure; D008099:Liver / Q000187:drug effects / Q000473:pathology; D051379:Mice; D009828:Oleanolic Acid / Q000008:administration & dosage / Q000494:pharmacology*; D009928:Organ Specificity / Q000187:drug effects; D011092:Polyethylene Glycols / Q000737:chemistry*; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D008081:Liposomes; D011092:Polyethylene Glycols; D009828:Oleanolic Acid