title: [Transcriptional regulation of exosporium basal layer structural gene exsB in Bacillus thuringiensis by SigmaK and GerE].
abstract: OBJECTIVE
  To identify the transcriptional regulation of exosporium basal layer structural gene exsB in Bacillus thuringiensis.
  
  
  METHODS
  We analyzed exsB and its promoter sequence in Bacillus cereus group by sequence alignment. We performed beta-galactosidase assay of exsB-lacZ gene fusion to analyze transcriptional activation of exsB promoter; we used Electrophoretic mobility shift assays to detect binding of GerE and exsB promoter.
  
  
  RESULTS
  exsB was the high similarity in Bacillus cereus group strains. Beta-galactosidase assay showed that exsB promoter had the strong transcriptional activation on the late sporulation phase. Deletion of gerE or sigK gene decreased the activation of exsB promoter. Electrophoretic mobility shift assays showed that GerE could bind with exsB promoter.
  
  
  CONCLUSIONS
  The exsB gene is regulated by SigmaK and GerE on the late sporulation phase.
authors.affiliation: College of Life Sciences, Northeast Agricultural University, Harbin 150030, China. xiaoshi_zia@126.com
authors.forename: Ning
authors.initials: N
authors.lastname: Qu
authors.forename: Qi
authors.initials: Q
authors.lastname: Peng
authors.forename: Lili
authors.initials: L
authors.lastname: Qiu
authors.forename: Chunxia
authors.initials: C
authors.lastname: Liu
authors.forename: Zhen
authors.initials: Z
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.forename: Fuping
authors.initials: F
authors.lastname: Song
authors.forename: Jie
authors.initials: J
authors.lastname: Li
mesh_terms: D001413:Bacillus thuringiensis / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D001665:Binding Sites; D016384:Consensus Sequence; D004269:DNA, Bacterial / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial / Q000235:genetics*; D009154:Mutation; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins; D016415:Sequence Alignment; D013171:Spores, Bacterial; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation; D001616:beta-Galactosidase
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; C084190:sigma K; C030069:spore-specific proteins, Bacillus; D001616:beta-Galactosidase