title: Distinct functional and temporal requirements for zebrafish Hdac1 during neural crest-derived craniofacial and peripheral neuron development.
abstract: The regulation of gene expression is accomplished by both genetic and epigenetic means and is required for the precise control of the development of the neural crest. In hdac1(b382) mutants, craniofacial cartilage development is defective in two distinct ways. First, fewer hoxb3a, dlx2 and dlx3-expressing posterior branchial arch precursors are specified and many of those that are consequently undergo apoptosis. Second, in contrast, normal numbers of progenitors are present in the anterior mandibular and hyoid arches, but chondrocyte precursors fail to terminally differentiate. In the peripheral nervous system, there is a disruption of enteric, DRG and sympathetic neuron differentiation in hdac1(b382) mutants compared to wildtype embryos. Specifically, enteric and DRG-precursors differentiate into neurons in the anterior gut and trunk respectively, while enteric and DRG neurons are rarely present in the posterior gut and tail. Sympathetic neuron precursors are specified in hdac1(b382) mutants and they undergo generic neuronal differentiation but fail to undergo noradrenergic differentiation. Using the HDAC inhibitor TSA, we isolated enzyme activity and temporal requirements for HDAC function that reproduce hdac1(b382) defects in craniofacial and sympathetic neuron development. Our study reveals distinct functional and temporal requirements for zebrafish hdac1 during neural crest-derived craniofacial and peripheral neuron development.
authors.affiliation: Molecular, Cellular and Developmental Biology Program, Ohio State University, Columbus, Ohio, United States of America.
authors.forename: Myron S
authors.initials: MS
authors.lastname: Ignatius
authors.forename: Arife
authors.initials: A
authors.lastname: Unal Eroglu
authors.forename: Smitha
authors.initials: S
authors.lastname: Malireddy
authors.forename: Glen
authors.initials: G
authors.lastname: Gallagher
authors.forename: Roopa M
authors.initials: RM
authors.lastname: Nambiar
authors.forename: Paul D
authors.initials: PD
authors.lastname: Henion
mesh_terms: D000818:Animals; D001934:Branchial Region / Q000002:abnormalities / Q000196:embryology / Q000473:pathology; D002454:Cell Differentiation / Q000187:drug effects; D019465:Craniofacial Abnormalities / Q000196:embryology / Q000473:pathology; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D005145:Face / Q000002:abnormalities / Q000196:embryology* / Q000473:pathology; D056284:Histone Deacetylase 1 / Q000235:genetics / Q000378:metabolism*; D006877:Hydroxamic Acids / Q000494:pharmacology; D006928:Hyoid Bone / Q000002:abnormalities / Q000187:drug effects / Q000196:embryology / Q000473:pathology; D008334:Mandible / Q000002:abnormalities / Q000187:drug effects / Q000196:embryology / Q000473:pathology; D009154:Mutation / Q000235:genetics; D009432:Neural Crest / Q000187:drug effects / Q000196:embryology / Q000378:metabolism / Q000473:pathology*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D017933:Peripheral Nervous System / Q000187:drug effects / Q000196:embryology / Q000473:pathology; D010641:Phenotype; D012886:Skull / Q000002:abnormalities / Q000196:embryology* / Q000473:pathology; D013234:Stem Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D013564:Sympathetic Nervous System / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D013997:Time Factors; D015027:Zebrafish / Q000196:embryology* / Q000378:metabolism*; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D006877:Hydroxamic Acids; D029961:Zebrafish Proteins; C012589:trichostatin A; C488363:HDAC1 protein, zebrafish; D056284:Histone Deacetylase 1