title: Antibiotic resistance determinants of multidrug-resistant Acinetobacter baumannii clinical isolates in Algeria.
abstract: Antibiotic susceptibility testing was performed on 71 Acinetobacter baumannii clinical isolates, and presence of antibiotic resistance genes was screened for by PCR amplification and sequencing. Resistance rates were very high for aminoglycosides (22-80%), fluoroquinolones (>90%), and cephalosporins (>90%) but remained low for rifampin (2.8%) or null for colistin. Antibiotic resistance encoding genes detected were as follows: blaTEM-128 gene (74.6%), aph(3')-VI (50.7 %), aadA (63.4%), ant(2″)-I (14.1%), aac(3)-Ia (91.1%), aac(6')-Ib (4.2%), mutation Ser83Leu in gyrA (94.4%), double mutations Ser83Leu and Ser80Leu (or Ser84Leu) in gyrA and parC (69.0%), and mutation I581N in RRDR of the rpoB gene.
authors.affiliation: Unité de recherche sur les maladies infectieuses et tropicales émergentes (URMITE) CNRS-IRD INSERM 7278, Méditerranée Infection, Faculté de Médecine et de Pharmacie, Aix-Marseille-Université, Marseille, France.
authors.forename: Sofiane
authors.initials: S
authors.lastname: Bakour
authors.forename: Abdelaziz
authors.initials: A
authors.lastname: Touati
authors.forename: Farida
authors.initials: F
authors.lastname: Sahli
authors.forename: Abdennour Ait
authors.initials: AA
authors.lastname: Ameur
authors.forename: Djamila
authors.initials: D
authors.lastname: Haouchine
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Rolain
mesh_terms: D000151:Acinetobacter Infections / Q000188:drug therapy* / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000145:classification / Q000627:therapeutic use*; D001426:Bacterial Proteins / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D006801:Humans; D008826:Microbial Sensitivity Tests; D009154:Mutation; D010802:Phylogeny
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins
keywords: Acinetobacter baumannii; Aminoglycoside-modifying enzymes; Multidrug resistant bacteria; Rifampin resistance