title: Lactobacillus planarum subsp. plantarum JCM 1149 vs. Aeromonas hydrophila NJ-1 in the anterior intestine and posterior intestine of hybrid tilapia Oreochromis niloticus ♀ × Oreochromis aureus ♂: an ex vivo study.
abstract: To investigate the ex vivo interactions of probiotic-pathogen-host in warm-water fish, hybrid tilapia (Oreochromis niloticus♀ × Oreochromis aureus♂) were sacrificed to isolate anterior and posterior intestine for incubation with phosphate-buffered saline (PBS; pH 7.2) as the control, Lactobacillus plantarum JCM 1149 at 1.0 × 10(9) CFU/ml, Aeromonas hydrophila NJ-1 at 1.0 × 10(8) CFU/ml, or the both combination. Denaturing Gradient Gel Electrophoresis (DGGE) fingerprint and consequent sequence analysis confirmed anterior intestine sac was more prone to the colonization of L. plantarum JCM 1149 and A. hydrophila NJ-1 than the posterior part. L. plantarum JCM 1149 and A. hydrophila NJ-1 inhibited the population each other in anterior or posterior sac, indicating their competition for the colonization. The induced expression of HSP70, IL-1β and TNF-α in the anterior sac by the addition of L. plantarum JCM 1149 or A. hydrophila NJ-1 demonstrated the activity and a local immune response of ex vivo anterior sac. Compared with posterior intestine, higher population colonization and more sensitive immune response of anterior sac indicated differential patterns for the probiotic-pathogen-host interactions. Scanning electronic microscopy (SEM) observation showed that pathogen A. hydrophila NJ-1 damaged the anterior intestine, which was alleviated by the pretreatment of L. plantarum JCM 1149, showing its probiotic effect.
authors.affiliation: Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Ren
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.forename: Yaling
authors.initials: Y
authors.lastname: Yang
authors.forename: Suxu
authors.initials: S
authors.lastname: He
authors.forename: Wenshu
authors.initials: W
authors.lastname: Liu
authors.forename: Einar
authors.initials: E
authors.lastname: Ringø
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Zhou
mesh_terms: D016980:Aeromonas hydrophila / Q000502:physiology*; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D058645:Denaturing Gradient Gel Electrophoresis / Q000662:veterinary; D006824:Hybridization, Genetic; D007422:Intestines / Q000382:microbiology*; D048191:Lactiplantibacillus plantarum / Q000502:physiology*; D008855:Microscopy, Electron, Scanning / Q000662:veterinary; D019936:Probiotics / Q000494:pharmacology*; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary; D017210:Tilapia / Q000235:genetics / Q000382:microbiology*