title: Histone deacetylases inhibitor trichostatin A modulates the extracellular release of APE1/Ref-1.
abstract: Apurinic/apyrimidinic endonuclease 1/Redox factor-1 (APE1/Ref-1) can be acetylated via post-translational modification. We investigated the effect of an inhibitor of histone deacetylases on the extracellular release of APE1/Ref-1 in HEK293 cells. Trichostatin A (TSA), an inhibitor of histone deacetylases, induced APE1/Ref-1 secretion without changing cell viability. In a fluorescence quantitative assay, the secreted APE1/Ref-1 was estimated to be about 10 ng/mL in response to TSA (1 μM). However, TSA did not induce the secretion of lysine-mutated APE1/Ref-1 (K6R/K7R). TSA also caused nuclear to cytoplasmic translocation of APE1/Ref-1. Taken together, these findings suggest that APE1/Ref-1 is a protein whose secretion is governed by lysine acetylation.
authors.affiliation: Department of Physiology, School of Medicine, Chungnam National University, Daejeon 301-747, Republic of Korea.
authors.forename: Sunga
authors.initials: S
authors.lastname: Choi
authors.forename: Yu Ran
authors.initials: YR
authors.lastname: Lee
authors.forename: Myoung Soo
authors.initials: MS
authors.lastname: Park
authors.forename: Hee Kyoung
authors.initials: HK
authors.lastname: Joo
authors.forename: Eun Jung
authors.initials: EJ
authors.lastname: Cho
authors.forename: Hyo Shin
authors.initials: HS
authors.lastname: Kim
authors.forename: Cuk Seong
authors.initials: CS
authors.lastname: Kim
authors.forename: Jin Bong
authors.initials: JB
authors.lastname: Park
authors.forename: Kaikobad
authors.initials: K
authors.lastname: Irani
authors.forename: Byeong Hwa
authors.initials: BH
authors.lastname: Jeon
mesh_terms: D000107:Acetylation / Q000187:drug effects; D021581:Active Transport, Cell Nucleus / Q000187:drug effects; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005110:Extracellular Space / Q000201:enzymology; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D057809:HEK293 Cells; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology*; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology*; D008239:Lysine / Q000737:chemistry; D016297:Mutagenesis, Site-Directed; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; D011993:Recombinant Fusion Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; C012589:trichostatin A; C085108:APEX1 protein, human; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase; D008239:Lysine