title: Roseovarius lutimaris sp. nov., isolated from a marine tidal flat.
abstract: A novel Gram-staining-negative, moderately halophilic and strictly aerobic bacterium, designated strain 112(T), was isolated from a tidal flat at Taean, Korea. Cells were catalase- and oxidase-positive ovoids to rods and did not produce bacteriochlorophyll a. Optimum growth of strain 112(T) was observed at 30 °C, at pH 6.5-7.5 and in the presence of 2-4 % (w/v) NaCl. The major cellular fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C16 : 0 and Q-10 was detected as the predominant ubiquinone. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, an unidentified aminolipid and three unidentified lipids. The genomic DNA G+C content of strain 112(T) was 58.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 112(T) formed a phyletic lineage within the genus Roseovarius of the class Alphaproteobacteria. Strain 112(T) was most closely related to Roseovarius mucosus DFL-24(T), with 96.52 % similarity. On the basis of phenotypic, chemotaxonomic and molecular properties, strain 112(T) represents a novel species of the genus Roseovarius, for which the name Roseovarius lutimaris sp. nov. is proposed. The type strain is 112(T) ( = KACC 16185(T) = JCM 17743(T)).
authors.affiliation: School of Biological Sciences and Research Center for Biomolecules and Biosystems, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Eun Jin
authors.initials: EJ
authors.lastname: Choi
authors.affiliation: School of Biological Sciences and Research Center for Biomolecules and Biosystems, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Hyo Jung
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: School of Biological Sciences and Research Center for Biomolecules and Biosystems, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Jeong Myeong
authors.initials: JM
authors.lastname: Kim
authors.affiliation: School of Biological Sciences and Research Center for Biomolecules and Biosystems, Chung-Ang University, Seoul 156-756, Republic of Korea.
authors.forename: Che Ok
authors.initials: CO
authors.lastname: Jeon
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D041902:Rhodobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000032:analysis; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2