title: Separation, immobilization, and biocatalytic utilization of proteins by a supramolecular membrane.
abstract: Membrane separation of biomolecules and their application in biocatalysis is becoming increasingly important for biotechnology, demanding the development of new biocompatible materials with novel properties. In the present study, an entirely noncovalent water-based material is used as a membrane for size-selective separation, immobilization, and biocatalytic utilization of proteins. The membrane shows stable performance under physiological conditions, allowing filtration of protein mixtures with a 150 kDa molecular weight cutoff (∼8 nm hydrodynamic diameter cutoff). Due to the biocompatibility of the membrane, filtered proteins stay functionally active and retained proteins can be partially recovered. Upon filtration, large enzymes become immobilized within the membrane. They exhibit stable activity when subjected to a constant flux of substrates for prolonged periods of time, which can be used to carry out heterogeneous biocatalysis. The noncovalent membrane material can be easily disassembled, purified, reassembled, and reused, showing reproducible performance after recycling. The robustness, recyclability, versatility, and biocompatibility of the supramolecular membrane may open new avenues for manipulating biological systems.
authors.affiliation: Department of Organic Chemistry, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: Elisha
authors.initials: E
authors.lastname: Krieg
authors.forename: Shira
authors.initials: S
authors.lastname: Albeck
authors.forename: Haim
authors.initials: H
authors.lastname: Weissman
authors.forename: Eyal
authors.initials: E
authors.lastname: Shimoni
authors.forename: Boris
authors.initials: B
authors.lastname: Rybtchinski
mesh_terms: D055162:Biocatalysis; D004800:Enzymes, Immobilized; D005663:Furans / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D007700:Kinetics; D008851:Micropore Filters; D008970:Molecular Weight; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D011728:Pyridones / Q000737:chemistry; D014462:Ultrafiltration / Q000379:methods*
chemical_list: D004800:Enzymes, Immobilized; D005663:Furans; D011506:Proteins; D011728:Pyridones; C543575:bolaamphiphile