title: Comparative proteomic analysis of aluminum tolerance in tibetan wild and cultivated barleys.
abstract: Aluminum (Al) toxicity is a major limiting factor for plant production in acid soils. Wild barley germplasm is rich in genetic diversity and may provide elite genes for crop Al tolerance improvement. The hydroponic-experiments were performed to compare proteomic and transcriptional characteristics of two contrasting Tibetan wild barley genotypes Al- resistant/tolerant XZ16 and Al-sensitive XZ61 as well as Al-resistant cv. Dayton. Results showed that XZ16 had less Al uptake and translocation than XZ61 and Dayton under Al stress. Thirty-five Al-tolerance/resistance-associated proteins were identified and categorized mainly in metabolism, energy, cell growth/division, protein biosynthesis, protein destination/storage, transporter, signal transduction, disease/defense, etc. Among them, 30 were mapped on barley genome, with 16 proteins being exclusively up-regulated by Al stress in XZ16, including 4 proteins (S-adenosylmethionine-synthase 3, ATP synthase beta subunit, triosephosphate isomerase, Bp2A) specifically expressed in XZ16 but not Dayton. The findings highlighted the significance of specific-proteins associated with Al tolerance, and verified Tibetan wild barley as a novel genetic resource for Al tolerance.
authors.affiliation: Department of Agronomy, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou, PR China.
authors.forename: Huaxin
authors.initials: H
authors.lastname: Dai
authors.forename: Fangbin
authors.initials: F
authors.lastname: Cao
authors.forename: Xianhong
authors.initials: X
authors.lastname: Chen
authors.forename: Mian
authors.initials: M
authors.lastname: Zhang
authors.forename: Imrul Mosaddek
authors.initials: IM
authors.lastname: Ahmed
authors.forename: Zhong-Hua
authors.initials: ZH
authors.lastname: Chen
authors.forename: Chengdao
authors.initials: C
authors.lastname: Li
authors.forename: Guoping
authors.initials: G
authors.lastname: Zhang
authors.forename: Feibo
authors.initials: F
authors.lastname: Wu
mesh_terms: D000535:Aluminum / Q000633:toxicity*; D046508:Culture Techniques; D005838:Genotype; D001467:Hordeum / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics; D040901:Proteomics; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics; D018609:Tibet; D059467:Transcriptome / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D010940:Plant Proteins; D000535:Aluminum