title: Determination of leakage from antibody adsorbent: composition analysis and pH effect.
abstract: To study the leakage at different solution pH values, IgG Sepharose 6FF®, a commercially available immunoadsorbent, was used as a model. The leaked substance consists of three parts: (1) ligands and its fragments; (2) ligands plus matrix fragments in which ligands are chemically attached to the adsorbent matrix; and (3) matrix fragments. Buffer solution pH values had a great effect on both the kinetics and the amount of ligand leakage. Cross-linking of the adsorbent matrix could reduce both matrix leakage and antibody leakage at pH 3.0, but its effect was limited at pH 11.0 for ligand leakage.
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.
authors.forename: Lan
authors.initials: L
authors.lastname: Zhao
authors.forename: Yongdong
authors.initials: Y
authors.lastname: Liu
authors.forename: Yinjue
authors.initials: Y
authors.lastname: Wang
authors.forename: Yongdong
authors.initials: Y
authors.lastname: Huang
authors.forename: Xiunan
authors.initials: X
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Guanghui
authors.initials: G
authors.lastname: Ma
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Su
mesh_terms: D000327:Adsorption; D000906:Antibodies / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000379:methods*; D019544:Equipment Failure Analysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007074:Immunoglobulin G / Q000737:chemistry; D008956:Models, Chemical; D012685:Sepharose / Q000737:chemistry
chemical_list: D000906:Antibodies; D007074:Immunoglobulin G; D012685:Sepharose
keywords: human immunoglobulin G; immunoadsorbent; ligand leakage; matrix leakage; pH-dependent