title: A likely role for a novel PH-domain containing protein, PEPP2, in connecting membrane and cytoskeleton.
abstract: PH domains (pleckstrin homology) are well known to bind membrane phosphoinositides with different specificities and direct PH domain-containing proteins to discrete subcellular apartments with assistances of alternative binding partners. PH domain-containing proteins are found to be involved in a wide range of cellular events, including signalling, cytoskeleton rearrangement and vesicular trafficking. Here we showed that a novel PH domain-containing protein, PEPP2, displayed moderate phosphoinositide binding specificity. Full length PEPP2 associated with both plasma membrane and microtubules. The membrane-associated PEPP2 nucleated at cell-cell contacts and the leading edge of migrating cells. Overexpression of PEPP2 increased membrane microviscosity, indicating a potential role of PEPP2 in regulating function of membrane and microtubules.
authors.affiliation: Department of Biology, School of Life Science and Technology, Jinan University, Guangzhou, China. tyizou@jnu.edu.cn
authors.forename: Yi
authors.initials: Y
authors.lastname: Zou
authors.forename: Wenping
authors.initials: W
authors.lastname: Zhong
mesh_terms: D000730:Androstadienes / Q000494:pharmacology; D000818:Animals; D019556:COS Cells; D002462:Cell Membrane / Q000378:metabolism*; D002522:Chlorocebus aethiops; D003599:Cytoskeleton / Q000378:metabolism*; D004058:Diffusion; D005982:Glutathione Transferase / Q000378:metabolism; D018398:Homeodomain Proteins / Q000378:metabolism*; D008055:Lipids / Q000737:chemistry; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000378:metabolism; D008954:Models, Biological; D010716:Phosphatidylinositols / Q000737:chemistry; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D015398:Signal Transduction; D014783:Viscosity; D000077191:Wortmannin; D014945:Wound Healing
chemical_list: D000730:Androstadienes; D018398:Homeodomain Proteins; D008055:Lipids; D010716:Phosphatidylinositols; C471968:RHOXF2 protein, human; D011993:Recombinant Fusion Proteins; D005982:Glutathione Transferase; D000077191:Wortmannin