title: A robust sensor platform for label-free detection of anti-Salmonella antibodies using undiluted animal sera.
abstract: We present a portable and easy-to-use biosensor platform, allowing for label-free detection of diagnostic markers in undiluted animal serum. Exemplarily, this is shown for the detection of anti-Salmonella antibodies. 1-lambda-Reflectometry was used as detection method, making the new biosensor platform portable, cheap, and robust. As recognition elements, lipopolysaccharides (LPSs) from Salmonella typhimurium bacteria were immobilized as sensitive layer on the transducer to carry out serological tests via a direct assay format. For this purpose, a new surface preparation protocol has been worked out allowing for immobilization of the LPS via hydrophobic interactions. It has been shown that results obtained by 1-lambda-Reflectometry are equivalent to those obtained by the non-portable Reflectometric Interference Spectroscopy setup. The new sensor platform was calibrated in both matrices, buffer and undiluted serum. Good sensitivity, selectivity and intra chip reproducibility have been observed. Furthermore, inter chip reproducibility was examined and recovery rates were found to be between 99 and 117% in undiluted serum.
authors.affiliation: Institute of Physical and Theoretical Chemistry (IPTC), Eberhard Karls Universität Tübingen, Tübingen, Germany. melanie.ewald@uni-tuebingen.de
authors.forename: Melanie
authors.initials: M
authors.lastname: Ewald
authors.forename: Alexander Fabian
authors.initials: AF
authors.lastname: Le Blanc
authors.forename: Günter
authors.initials: G
authors.lastname: Gauglitz
authors.forename: Günther
authors.initials: G
authors.lastname: Proll
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D012475:Salmonella / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial