title: Sequence and phylogenetic analyses of the water-soluble glucan synthesizing-glucosyltransferase genes of Streptococcus dentirousetti.
abstract: Two tandemly aligned glucosyltransferase (GTF) genes whose gene products are responsible for water-soluble glucan synthesis were isolated from Streptococcus dentirousetti NUM1303 and sequenced. One of the GTF genes of S. dentirousetti consisted of a 4110 bp open reading frame (ORF) that encoded for a 1369 amino acid protein and was revealed to be a S. sobrinus gtfS homolog. The percent similarity of amino acid sequences of the GTF-S from S. dentirousetti compared to those from S. sobrinus was 99%. In addition, a putative gtfT was found in tandem in the downstream region of the S. dentirousetti gtfS. The gtfT of S. dentirousetti consisted of a 4527 bp ORF encoding for 1508 amino acids. The similarity of amino acid sequences of the GTF-T from S. dentirousetti and S. sobrinus was 94%. Phylogenetic analysis based on amino acid sequences from other related streptococcal GTFs suggested that both GTF-S and GTF-T of S. dentirousetti are closely related to S. sobrinus.
authors.affiliation: Department of Oral Microbiology, Nihon University School of Dentistry at Matsudo, Chiba 271-8587, Japan. kuwahara.noriko@nihon‐u.ac.jp
authors.forename: Noriko
authors.initials: N
authors.lastname: Shinozaki-Kuwahara
authors.forename: Masanori
authors.initials: M
authors.lastname: Saito
authors.forename: Masatomo
authors.initials: M
authors.lastname: Hirasawa
authors.forename: Kazuko
authors.initials: K
authors.lastname: Takada
mesh_terms: D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D005936:Glucans / Q000096:biosynthesis*; D005964:Glucosyltransferases / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D013291:Streptococcus / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D005936:Glucans; D005964:Glucosyltransferases