title: GnRH neuron type-specific transcriptome analysis by laser captured single-cell microarray in the medaka.
abstract: Most vertebrates possess at least two gonadotropin-releasing hormone (GnRH) neuron types. To understand the physiological significance of the multiple GnRH systems in the brain, we examined three GnRH neuron type-specific transcriptomes using single-cell microarray analyses in the medaka (Oryzias latipes). A microarray profile of the three GnRH neuron types revealed five genes that are uniquely expressed in specific GnRH neuron types. GnRH1 neurons expressed three genes that are homologous to functionally characterised genes, GnRH2 neurons uniquely expressed one unnamed gene, and GnRH3 neurons uniquely expressed one known gene. These genes may be involved in the modulation or maintenance of each GnRH neuron type.
authors.affiliation: Brain Research Institute, Monash University Sunway Campus, Jalan Lagoon Selatan, Bandar Sunway, Selangor 46150, Malaysia.
authors.forename: Shogo
authors.initials: S
authors.lastname: Moriya
authors.forename: Satoshi
authors.initials: S
authors.lastname: Ogawa
authors.forename: Ishwar S
authors.initials: IS
authors.lastname: Parhar
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002469:Cell Separation / Q000379:methods; D002478:Cells, Cultured; D020869:Gene Expression Profiling / Q000379:methods*; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D008297:Male; D009474:Neurons / Q000378:metabolism*; D052898:Optical Tweezers; D009990:Oryzias / Q000378:metabolism*; D040081:Protein Array Analysis / Q000379:methods*; D059467:Transcriptome / Q000502:physiology*
chemical_list: D007987:Gonadotropin-Releasing Hormone