title: Semiautomated analysis of dendrite morphology in cell culture.
abstract: Quantifying dendrite morphology is a method for determining the effect of biochemical pathways and extracellular agents on neuronal development and differentiation. Quantification can be performed using Sholl analysis, dendrite counting, and length quantification. These procedures can be performed on dendrite-forming cell lines or primary neurons grown in culture. In this protocol, we describe the use of a set of computer programs to assist in quantifying many aspects of dendrite morphology, including changes in total and localized arbor complexity.
authors.affiliation: Neuroscience Graduate Program, Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, NJ, USA.
authors.forename: Eric S
authors.initials: ES
authors.lastname: Sweet
authors.forename: Chris L
authors.initials: CL
authors.lastname: Langhammer
authors.forename: Melinda K
authors.initials: MK
authors.lastname: Kutzing
authors.forename: Bonnie L
authors.initials: BL
authors.lastname: Firestein
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001331:Automation; D018929:Cell Culture Techniques / Q000379:methods*; D003712:Dendrites / Q000378:metabolism*; D012984:Software; D013223:Statistics as Topic