title: Host and viral features of human dengue cases shape the population of infected and infectious Aedes aegypti mosquitoes.
abstract: Dengue is the most prevalent arboviral disease of humans. The host and virus variables associated with dengue virus (DENV) transmission from symptomatic dengue cases (n = 208) to Aedes aegypti mosquitoes during 407 independent exposure events was defined. The 50% mosquito infectious dose for each of DENV-1-4 ranged from 6.29 to 7.52 log10 RNA copies/mL of plasma. Increasing day of illness, declining viremia, and rising antibody titers were independently associated with reduced risk of DENV transmission. High early DENV plasma viremia levels in patients were a marker of the duration of human infectiousness, and blood meals containing high concentrations of DENV were positively associated with the prevalence of infectious mosquitoes 14 d after blood feeding. Ambulatory dengue cases had lower viremia levels compared with hospitalized dengue cases but nonetheless at levels predicted to be infectious to mosquitoes. These data define serotype-specific viremia levels that vaccines or drugs must inhibit to prevent DENV transmission.
authors.affiliation: Oxford University Clinical Research Unit, District 5, Ho Chi Minh City, Vietnam.
authors.forename: Minh Nguyen
authors.initials: MN
authors.lastname: Nguyet
authors.forename: Thi Hue Kien
authors.initials: TH
authors.lastname: Duong
authors.forename: Vu Tuan
authors.initials: VT
authors.lastname: Trung
authors.forename: Than Ha Quyen
authors.initials: TH
authors.lastname: Nguyen
authors.forename: Chau N B
authors.initials: CN
authors.lastname: Tran
authors.forename: Vo Thi
authors.initials: VT
authors.lastname: Long
authors.forename: Le Thi
authors.initials: le T
authors.lastname: Dui
authors.forename: Hoa Lan
authors.initials: HL
authors.lastname: Nguyen
authors.forename: Jeremy J
authors.initials: JJ
authors.lastname: Farrar
authors.forename: Edward C
authors.initials: EC
authors.lastname: Holmes
authors.forename: Maia A
authors.initials: MA
authors.lastname: Rabaa
authors.forename: Juliet E
authors.initials: JE
authors.lastname: Bryant
authors.forename: Truong Thanh
authors.initials: TT
authors.lastname: Nguyen
authors.forename: Huong Thi Cam
authors.initials: HT
authors.lastname: Nguyen
authors.forename: Lan Thi Hong
authors.initials: LT
authors.lastname: Nguyen
authors.forename: Mai Phuong
authors.initials: MP
authors.lastname: Pham
authors.forename: Hung The
authors.initials: HT
authors.lastname: Nguyen
authors.forename: Tai Thi Hue
authors.initials: TT
authors.lastname: Luong
authors.forename: Bridget
authors.initials: B
authors.lastname: Wills
authors.forename: Chau Van Vinh
authors.initials: CV
authors.lastname: Nguyen
authors.forename: Marcel
authors.initials: M
authors.lastname: Wolbers
authors.forename: Cameron P
authors.initials: CP
authors.lastname: Simmons
mesh_terms: D000330:Aedes / Q000821:virology*; D000818:Animals; D001483:Base Sequence; D015331:Cohort Studies; D003715:Dengue / Q000453:epidemiology* / Q000635:transmission* / Q000821:virology*; D003716:Dengue Virus / Q000145:classification / Q000235:genetics*; D004797:Enzyme-Linked Immunosorbent Assay; D054884:Host-Pathogen Interactions; D006801:Humans; D007303:Insect Vectors / Q000821:virology*; D016013:Likelihood Functions; D008957:Models, Genetic; D008969:Molecular Sequence Data; D010802:Phylogeny; D015995:Prevalence; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012703:Serotyping; D014744:Vietnam / Q000453:epidemiology; D014766:Viremia / Q000453:epidemiology
keywords: entomology; infectious disease; virology