title: [Preliminary study on pH-sensitive lipid bilayer-coated mesoporous silica nanoparticles as a novel drug carrier for antitumor drug].
abstract: This study plans to prepare lipid bilayer-coated mesoporous silica nanoparticles (LMSNs) which are pH sensitive with core-shell structure to improve the tumor cell lethality of antitumor drug. The lipid coated mesoporous silica nanoparticles loaded with irinotecan (CPT-11) (CPT-11-LMSNs) were prepared by hot water-film hydration method, and the characterized its morphology, particle size and release in vitro. Meanwhile, the intracellular uptake and cell toxicity of CPT-11-LMSNs and intracellular accumulation of CPT-11 were evaluated on human breast carcinoma cell line (MCF-7). The results indicated that the mean diameter of the spherical LMSNs was (120.27 +/- 5.91) nm. The slow release in simulated normal physiological conditions and a rapid release under simulated intracellular condition demonstrated the pH sensitivity of CPT-11-MSNs in vitro. Moreover, the CPT-11-LMSN could improve the intracellular CPT-11 cumulant 2.1 times and reduce half maximal inhibitory concentration (IC50) values of CPT-11 1.4 times compared with CPT-11-MSNs, demonstrating a stronger cell lethality.
authors.affiliation: Department of Pharmaceutics, China Pharmaceutical University, Nanjing 210009, China.
authors.forename: Fei-Fei
authors.initials: FF
authors.lastname: Li
authors.forename: Xin-Xin
authors.initials: XX
authors.lastname: Zhang
authors.forename: Shi-Yan
authors.initials: SY
authors.lastname: Guo
authors.forename: Yong
authors.initials: Y
authors.lastname: Gan
authors.forename: Juan
authors.initials: J
authors.lastname: Li
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage* / Q000494:pharmacology; D002166:Camptothecin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D004337:Drug Carriers; D006801:Humans; D006863:Hydrogen-Ion Concentration; D000077146:Irinotecan; D008051:Lipid Bilayers / Q000008:administration & dosage / Q000737:chemistry*; D061986:MCF-7 Cells; D053758:Nanoparticles; D010316:Particle Size; D016062:Porosity; D012822:Silicon Dioxide / Q000008:administration & dosage / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004337:Drug Carriers; D008051:Lipid Bilayers; D012822:Silicon Dioxide; D000077146:Irinotecan; D002166:Camptothecin