title: Metabolomics-based component profiling of Halomonas sp. KM-1 during different growth phases in poly(3-hydroxybutyrate) production.
abstract: To investigate the relationship between the production of poly(3-hydroxybutyrate) (PHB) and metabolic changes during different growth phases, a non-sterile batch fermentation process involving an alkalophilic and halophilic bacterium, Halomonas sp. KM-1, was used. Intracellular metabolites were analyzed using gas chromatography-mass spectrometry to characterize the metabolic profile. Significant changes relating to PHB production were observed in the TCA cycle, lipid-synthesis and amino acid biosynthetic pathways were found to shift dramatically between the exponential growth and stationary phases. During the stationary phase, 17 metabolites were upregulated and a cell dry mass of 17.8 g/L that included 44.8% PHB was observed at 24h in 5% glucose-supplemented cultures, whereas 11 metabolites were upregulated and a cell dry mass of 38.4 g/L that included 73.7% PHB was observed at 36 h in 10% glucose-supplemented cultures. This study provides pattern analysis of metabolite regulation during PHB accumulation, indicating that multicomponent and phase-specific mechanisms are involved.
authors.affiliation: Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Midorigaoka, Ikeda, Osaka 563-8577, Japan.
authors.forename: You-Xun
authors.initials: YX
authors.lastname: Jin
authors.forename: Lian-Hua
authors.initials: LH
authors.lastname: Shi
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Kawata
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D061249:Batch Cell Culture Techniques; D018533:Biomass; D008401:Gas Chromatography-Mass Spectrometry; D005947:Glucose / Q000494:pharmacology; D020632:Halomonas / Q000254:growth & development* / Q000378:metabolism*; D006885:Hydroxybutyrates / Q000378:metabolism*; D008055:Lipids / Q000096:biosynthesis; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D055432:Metabolomics / Q000379:methods*; D050296:Microbial Viability / Q000187:drug effects; D011091:Polyesters / Q000378:metabolism*; D013997:Time Factors; D000094122:Polyhydroxybutyrates
chemical_list: D000596:Amino Acids; D005947:Glucose; D006885:Hydroxybutyrates; D008055:Lipids; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates