title: Characterization of genes required for the pathogenicity of Pectobacterium carotovorum subsp. carotovorum Pcc21 in Chinese cabbage.
abstract: Pectobacterium carotovorum subsp. carotovorum is a well-known plant pathogen that causes severe soft rot disease in various crops, resulting in considerable economic loss. To identify pathogenicity-related factors, Chinese cabbage was inoculated with 5314 transposon mutants of P. carotovorum subsp. carotovorum Pcc21 derived using Tn5 transposon mutagenesis. A total of 35 reduced-virulence or avirulent mutants were isolated, and 14 loci were identified. The 14 loci could be functionally grouped into nutrient utilization (pyrD, purH, purD, leuA and serB), production of plant cell-wall-degrading enzymes (PCWDEs) (expI, expR and PCC21_023220), motility (flgA, fliA and flhB), biofilm formation (expI, expR and qseC), susceptibility to antibacterial plant chemicals (tolC) and unknown function (ECA2640). Among the 14 genes identified, qseC, tolC and PCC21_023220 are novel pathogenicity factors of P. carotovorum subsp. carotovorum involved in biofilm formation, phytochemical resistance and PCWDE production, respectively.
authors.affiliation: Division of Microbial Safety, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
authors.forename: Dong Hwan
authors.initials: DH
authors.lastname: Lee
authors.affiliation: Division of Microbial Safety, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
authors.forename: Jeong-A
authors.initials: JA
authors.lastname: Lim
authors.affiliation: Division of Microbial Safety, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
authors.forename: Juneok
authors.initials: J
authors.lastname: Lee
authors.affiliation: Division of Microbial Safety, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
authors.forename: Eunjung
authors.initials: E
authors.lastname: Roh
authors.affiliation: Division of Microbial Safety, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
authors.forename: Kyusuk
authors.initials: K
authors.lastname: Jung
authors.affiliation: Department of Horticultural Biotechnology and Institute of Life Science & Resources, Kyung Hee University, Yongin 441-701, Republic of Korea.
authors.forename: Minseon
authors.initials: M
authors.lastname: Choi
authors.affiliation: Department of Horticultural Biotechnology and Institute of Life Science & Resources, Kyung Hee University, Yongin 441-701, Republic of Korea.
authors.forename: Changsik
authors.initials: C
authors.lastname: Oh
authors.affiliation: Department of Agricultural Biotechnology, Center for Agricultural Biomaterials, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea.
authors.forename: Sangryeol
authors.initials: S
authors.lastname: Ryu
authors.affiliation: Division of Microbial Safety, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
authors.forename: Jongchul
authors.initials: J
authors.lastname: Yun
authors.affiliation: Division of Microbial Safety, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
authors.forename: Sunggi
authors.initials: S
authors.lastname: Heu
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D018441:Biofilms / Q000254:growth & development; D001937:Brassica / Q000382:microbiology*; D004251:DNA Transposable Elements / Q000235:genetics; D024881:Drug Resistance, Bacterial; D015964:Gene Expression Regulation, Bacterial; D008826:Microbial Sensitivity Tests; D016254:Mutagenesis, Insertional; D016973:Pectobacterium carotovorum / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D010935:Plant Diseases / Q000382:microbiology*; D014774:Virulence / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D037521:Virulence Factors