title: Cytotoxicity of α-dicarbonyl compounds submitted to in vitro simulated digestion process.
abstract: α-Dicarbonyl compounds (α-DCs), such as glyoxal, methylglyoxal and 2,3-butanedione, are highly reactive substances occurring in thermally treated and fermented foods, that may react with amino and sulphydryl groups of side chains of proteins to form Maillard reaction end products, inducing a negative impact on the digestibility and on nutritional value of protein. In recent years the role of food derived α-DCs in gastroduodenal tract is under investigation to understand whether excess consumption of such dietary compounds might be a risk for human health. In this study the interactions between a mixture of glyoxal, methylglyoxal and 2,3-butanedione and the digestive enzymes (pepsin and pancreatin) were studied. The results showed that during gastroduodenal digestion α-DCs react with digestive enzymes to produce carbonylated proteins. Moreover, undigested and digested α-DC cytotoxicity against human cells, as well as their ability to inhibit the function of human enzymes responsible for DNA repair were shown.
authors.affiliation: Institute of Molecular Genetics, IGM-CNR, via Abbiategrasso 207, 27100 Pavia, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Amoroso
authors.forename: Giovanni
authors.initials: G
authors.lastname: Maga
authors.forename: Maria
authors.initials: M
authors.lastname: Daglia
mesh_terms: D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D019951:DNA Polymerase beta / Q000037:antagonists & inhibitors / Q000378:metabolism; D003931:Diacetyl / Q000633:toxicity*; D004063:Digestion; D006037:Glyoxal / Q000633:toxicity*; D006801:Humans; D008954:Models, Biological; D010194:Pancreatin / Q000037:antagonists & inhibitors / Q000378:metabolism*; D010434:Pepsin A / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D050050:Protein Carbonylation / Q000187:drug effects; D011765:Pyruvaldehyde / Q000633:toxicity*
chemical_list: D006037:Glyoxal; D011765:Pyruvaldehyde; D010194:Pancreatin; C415367:DNA polymerase beta2; D019951:DNA Polymerase beta; D010434:Pepsin A; D003931:Diacetyl