title: Protective mechanisms of sevoflurane against one-lung ventilation-induced acute lung injury: role of cyclooxygenase-2 and 5-lipoxygenase pathways.
abstract: OBJECTIVE
  To explore the protective mechanisms of sevoflurane against acute lung injury (ALI) induced by one-lung ventilation (OLV) in view of cyclooxygenase-2 (COX2) and 5-lipoxygenase (5-LOX) pathways.
  
  
  METHODS
  Eighteen healthy Japanese white rabbits were randomized into sham-operated group (S group), OLV group (O group) and OLV + sevoflurane group (OS group). COX2 and 5-LOX protein and mRNA expressions in the lungs were detected by Western blotting and real-time PCR, respectively. Prostaglandin I2 (PGI2), thromboxane A2 (TXA2) and leukotrienes B2 (LTB2) in the lung tissues were quantified with ELISA. Histological scores and lung wet/dry weight (W/D) ratios were determined for lung injury assessment.
  
  
  RESULTS
  COX2 and 5-LOX protein and mRNA expressions and the contents of LTB2, TXA2 and PGI2 in the lungs, lung W/D ratio and histological scores were significantly higher while PGI2/TXA2 ratio was significantly lower in O group and OS group than in S group (P<0.05). Compared with those in O group, COX2 and 5-LOX expressions, pulmonary contents of LTB2, TXA2 and PGI2, and lung W/D ratio all decreased significantly but PGI2/TXA2 ratio was significantly elevated in OS group (P<0.05).
  
  
  CONCLUSIONS
  OLV may activate COX2 and 5-LOX pathways to result in increased production of arachidonic acid metabolites. Sevoflurane protects against OLV-induced ALI probably by reducing AA metabolites and regulating PGI2/TXA2 ratio through inhibitions of COX2 and 5-LOX pathways.
authors.affiliation: Kunming Medical University, Kunming, China. liur414324@hotmail.com
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Luo
authors.forename: Jiang
authors.initials: J
authors.lastname: Li
authors.forename: Qingjie
authors.initials: Q
authors.lastname: Ma
authors.forename: Jingzhe
authors.initials: J
authors.lastname: Sun
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Li
authors.forename: Dianhua
authors.initials: D
authors.lastname: Wang
mesh_terms: D055371:Acute Lung Injury / Q000209:etiology / Q000378:metabolism*; D000818:Animals; D001094:Arachidonate 5-Lipoxygenase / Q000378:metabolism*; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D008168:Lung / Q000187:drug effects / Q000378:metabolism; D008738:Methyl Ethers / Q000009:adverse effects*; D061810:One-Lung Ventilation / Q000009:adverse effects*; D012333:RNA, Messenger / Q000235:genetics; D011817:Rabbits; D000077149:Sevoflurane
chemical_list: D008738:Methyl Ethers; D012333:RNA, Messenger; D000077149:Sevoflurane; D001094:Arachidonate 5-Lipoxygenase; D051546:Cyclooxygenase 2