title: [Influencing of hep-2 cell function by RNAi silencing E-cadherin expression].
abstract: OBJECTIVE
  To explore the function of human laryngeal carcinoma Hep-2 cell after down-regulating the expression of E-cadherin gene to provide theoretical rationales for gene therapy of laryngeal cancer.
  
  
  METHODS
  According to the GenBank database, 3 pairs of shRNA sequences of E-cadherin gene were designed and synthesized. shRNAs were transfected into the cell line Hep-2 by liposome. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the silencing effect of E-cadherin expression. The changed capacity of cell proliferation were detected in vitro by methyl thiazolyl tetrazolium (MTT) assay in the transfected Hep-2 cells and the cell proliferation rate (survival rate) was calculated. And Transwell was used to detect the migratory capacity of Hep-2 cells after siRNA transfection.
  
  
  RESULTS
  The E-cadherin gene expression of RNAi transfected Hep-2 cells significantly decreased in interference group. And the proliferation of interference group became markedly enhanced. In Transwell test, the migrated cell numbers in interference group were significant higher than those in negative control group (262 ± 15, 288 ± 12, 292 ± 6 vs 74 ± 8, all P < 0.01).
  
  
  CONCLUSIONS
  RNA interference silencing of E-cadherin gene expression can significantly enhance the proliferation and migratory capacity of Hep-2 cells. And E-cadherin may be considered as one of gene therapy targets for laryngeal cancer.
authors.affiliation: Department of Otorhinolaryngology, Weifang People's Hospital, Weifang 261041, China. tjunmail@126.com
authors.forename: Jun
authors.initials: J
authors.lastname: Tian
authors.forename: Cheng-wen
authors.initials: CW
authors.lastname: Li
authors.forename: Gui-qing
authors.initials: GQ
authors.lastname: Wu
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.forename: Qi
authors.initials: Q
authors.lastname: Chen
mesh_terms: D015703:Antigens, CD; D015820:Cadherins / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D009361:Neoplasm Invasiveness; D034622:RNA Interference
chemical_list: D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins