title: A new Schiff base fluorescent probe for imaging Cu2+ in living cells.
abstract: A novel probe based on ferrocenyl-1,3,4-thiadiazol-containing Schiff base was synthesized by the reaction of 5-ferrocenyl-1,3,4-thiadiazol-2-amine and 4-(diethylamino)salicylaldehyde, and characterized by IR, NMR, HRMS and X-ray analysis. UV-vis spectral and fluorescence property of the probe were investigated. The probe can be used to colorimetric sensitive and selective fluorescent recognition of Cu(2+) in buffer solution. Moreover, the probe can detect Cu(2+) by electrochemical method. Additionally, the Schiff base was successfully used as a selective and sensitive fluorescent probe for monitoring Cu(2+) ions in living cells.
authors.affiliation: Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Ye
authors.forename: Fei
authors.initials: F
authors.lastname: Ge
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Zhou
authors.forename: Jin-Ting
authors.initials: JT
authors.lastname: Liu
authors.forename: Bao-Xiang
authors.initials: BX
authors.lastname: Zhao
mesh_terms: D003124:Colorimetry / Q000379:methods; D003300:Copper / Q000032:analysis*; D005296:Ferrous Compounds / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D057230:Limit of Detection; D008856:Microscopy, Fluorescence; D008958:Models, Molecular; D012545:Schiff Bases / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013830:Thiadiazoles / Q000737:chemistry*
chemical_list: D005296:Ferrous Compounds; D005456:Fluorescent Dyes; D012545:Schiff Bases; D013830:Thiadiazoles; C058949:1,3,4-thiadiazole; D003300:Copper