title: Electrochemical impedance spectroscopic sensing of methamphetamine by a specific aptamer.
abstract: BACKGROUND
  Electrochemical impedance spectroscopy (EIS) is a simple and highly sensitive technique that can be used for evaluation of the aptamer-target interaction even in a label-free approach.
  
  
  METHODS
  To pursue the effectiveness of EIS, in the current study, the folding properties of specific aptamer for methamphetamine (METH) (i.e., aptaMETH) were evaluated in the presence of METH and amphetamine (Amph). Folded and unfolded aptaMETH was mounted on the gold electrode surface and the electron charge transfer was measured by EIS.
  
  
  RESULTS
  The Ret of methamphetamine-aptaMETH was significantly increased in comparison with other folding conditions, indicating specific detection of METH by aptaMETH.
  
  
  CONCLUSIONS
  Based on these findings, methamphetamine-aptaMETH on the gold electrode surface displayed the most interfacial electrode resistance and thus the most folding situation. This clearly indicates that the aptaMETH can profoundly and specifically pinpoint METH; as a result we suggest utilization of this methodology for fast and cost-effective identification of METH.
authors.affiliation: Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran ; Students' Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran ; Research Center for Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Ebrahimi
authors.forename: Mohammad
authors.initials: M
authors.lastname: Johari-Ahar
authors.forename: Hossein
authors.initials: H
authors.lastname: Hamzeiy
authors.forename: Jaleh
authors.initials: J
authors.lastname: Barar
authors.forename: Omid
authors.initials: O
authors.lastname: Mashinchian
authors.forename: Yadollah
authors.initials: Y
authors.lastname: Omidi
keywords: Aptamer; Biosensor; Electrochemical Impedance; Gold Nanoparticles; Spectroscopy