title: Isolation and characterization of fatty acid methyl ester (FAME)-producing Streptomyces sp. S161 from sheep (Ovis aries) faeces.
abstract: An actinomycete producing oil-like mixtures was isolated and characterized. The strain was isolated from sheep faeces and identified as Streptomyces sp. S161 based on 16S rRNA gene sequence analysis. The strain showed cellulase and xylanase activities. The (1) H nuclear magnetic resonance (NMR) spectra of the mixtures showed that the mixtures were composed of fatty acid methyl esters (52·5), triglycerides (13·7) and monoglycerides (9·1) (mol.%). Based on the gas chromatography-mass spectrometry (GC-MS) analysis, the fatty acid methyl esters were mainly composed of C14-C16 long-chain fatty acids. The results indicated that Streptomyces sp. S161 could produce fatty acid methyl esters (FAME) directly from starch. To our knowledge, this is the first isolated strain that can produce biodiesel (FAME) directly from starch.
authors.affiliation: School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Lu
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: Z
authors.initials: Z
authors.lastname: Deng
authors.forename: H
authors.initials: H
authors.lastname: Wu
authors.forename: Q
authors.initials: Q
authors.lastname: Deng
authors.forename: H
authors.initials: H
authors.lastname: Tan
authors.forename: L
authors.initials: L
authors.lastname: Cao
mesh_terms: D000818:Animals; D056804:Biofuels; D005227:Fatty Acids / Q000096:biosynthesis* / Q000737:chemistry; D005243:Feces / Q000382:microbiology*; D008401:Gas Chromatography-Mass Spectrometry; D009682:Magnetic Resonance Spectroscopy; D050178:Monoglycerides / Q000096:biosynthesis / Q000737:chemistry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D034561:Sheep, Domestic / Q000382:microbiology*; D013213:Starch / Q000378:metabolism; D013302:Streptomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014280:Triglycerides / Q000096:biosynthesis / Q000737:chemistry
chemical_list: D056804:Biofuels; D005227:Fatty Acids; D050178:Monoglycerides; D012336:RNA, Ribosomal, 16S; D014280:Triglycerides; D013213:Starch
keywords: 1
  H NMR; Streptomyces; biodiesel; fatty acid methyl ester; sheep