title: Localized surface plasmon resonance detection of biological toxins using cell surface oligosaccharides on glyco chips.
abstract: We have detected biological toxins using localized surface plasmon resonance (LSPR) and synthetic glycosyl ceramides (β-lactoside, globosyl trisaccharide (Gb3), or GM1 pentasaccharide) attached to gold (Au) nanoparticles. The particle diameters ranged from 5-100 nm. The detection sensitivity for three toxins (ricin, Shiga toxin, and cholera toxin) was found to depend not only on the attached glycoside but also on the diameter of the Au nanoparticles. For the detection of ricin, the 20-nm β-lactoside-coated Au nanoparticle exhibited the highest LSPR response, whereas 40-nm Gb3- and GM1-coated Au nanoparticles gave the best results for Shiga toxin and cholera toxin, respectively. In addition, a blocking process on the nanoparticle surface greatly improved the detection sensitivity for cholera toxin. The LSPR system enabled us to detect ricin at 30 ng/mL, Shiga toxin at 10 ng/mL, and the cholera toxin at 20 ng/mL.
authors.affiliation: Nanosystem Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Japan.
authors.forename: Takehiro
authors.initials: T
authors.lastname: Nagatsuka
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Uzawa
authors.forename: Keita
authors.initials: K
authors.lastname: Sato
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kondo
authors.forename: Masayuki
authors.initials: M
authors.lastname: Izumi
authors.forename: Kenji
authors.initials: K
authors.lastname: Yokoyama
authors.forename: Isaac
authors.initials: I
authors.lastname: Ohsawa
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Seto
authors.forename: Paola
authors.initials: P
authors.lastname: Neri
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mori
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Nishida
authors.forename: Masato
authors.initials: M
authors.lastname: Saito
authors.forename: Eiichi
authors.initials: E
authors.lastname: Tamiya
mesh_terms: D002462:Cell Membrane / Q000737:chemistry; D004705:Endocytosis; D056656:Lab-On-A-Chip Devices; D009844:Oligosaccharides / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000379:methods*; D014118:Toxins, Biological / Q000032:analysis*
chemical_list: D009844:Oligosaccharides; D014118:Toxins, Biological