title: Cyclophilin 20-3 relays a 12-oxo-phytodienoic acid signal during stress responsive regulation of cellular redox homeostasis.
abstract: The jasmonate family of phytohormones plays central roles in plant development and stress acclimation. However, the architecture of their signaling circuits remains largely unknown. Here we describe a jasmonate family binding protein, cyclophilin 20-3 (CYP20-3), which regulates stress-responsive cellular redox homeostasis. (+)-12-Oxo-phytodienoic acid (OPDA) binding promotes CYP20-3 to form a complex with serine acetyltransferase 1, which triggers the formation of a hetero-oligomeric cysteine synthase complex with O-acetylserine(thiol)lyase B in chloroplasts. The cysteine synthase complex formation then activates sulfur assimilation that leads to increased levels of thiol metabolites and the buildup of cellular reduction potential. The enhanced redox capacity in turn coordinates the expression of a subset of OPDA-responsive genes. Thus, we conclude that CYP20-3 is a key effector protein that links OPDA signaling to amino acid biosynthesis and cellular redox homeostasis in stress responses.
authors.affiliation: Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.
authors.forename: Sang-Wook
authors.initials: SW
authors.lastname: Park
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Andrea
authors.initials: A
authors.lastname: Viehhauser
authors.forename: Bin
authors.initials: B
authors.lastname: He
authors.forename: Soonok
authors.initials: S
authors.lastname: Kim
authors.forename: Anders K
authors.initials: AK
authors.lastname: Nilsson
authors.forename: Mats X
authors.initials: MX
authors.lastname: Andersson
authors.forename: Joshua D
authors.initials: JD
authors.lastname: Kittle
authors.forename: Madana M R
authors.initials: MM
authors.lastname: Ambavaram
authors.forename: Sheng
authors.initials: S
authors.lastname: Luan
authors.forename: Alan R
authors.initials: AR
authors.lastname: Esker
authors.forename: Dorothea
authors.initials: D
authors.lastname: Tholl
authors.forename: Daniela
authors.initials: D
authors.lastname: Cimini
authors.forename: Mats
authors.initials: M
authors.lastname: Ellerström
authors.forename: Gitta
authors.initials: G
authors.lastname: Coaker
authors.forename: Thomas K
authors.initials: TK
authors.lastname: Mitchell
authors.forename: Andy
authors.initials: A
authors.lastname: Pereira
authors.forename: Karl-Josef
authors.initials: KJ
authors.lastname: Dietz
authors.forename: Christopher B
authors.initials: CB
authors.lastname: Lawrence
mesh_terms: D000596:Amino Acids / Q000096:biosynthesis; D017360:Arabidopsis; D002736:Chloroplasts / Q000378:metabolism*; D002846:Chromatography, Affinity; D003517:Cyclopentanes / Q000378:metabolism; D021983:Cyclophilins / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D006706:Homeostasis / Q000502:physiology*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology*; D054883:Oxylipins / Q000378:metabolism; D060066:Protein Interaction Maps; D051050:Serine O-Acetyltransferase / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D000596:Amino Acids; D003517:Cyclopentanes; D005231:Fatty Acids, Unsaturated; D054883:Oxylipins; C025999:12-oxophytodienoic acid; C011006:jasmonic acid; D051050:Serine O-Acetyltransferase; D021983:Cyclophilins
keywords: GSH; TGA; cysteine biosynthesis; plant plasticity; wounding