title: [Principles for molecular identification of traditional Chinese materia medica using DNA barcoding].
abstract: Since the research of molecular identification of Chinese Materia Medica (CMM) using DNA barcode is rapidly developing and popularizing, the principle of this method is approved to be listed in the Supplement of the Pharmacopoeia of the People's Republic of China. Based on the study on comprehensive samples, the DNA barcoding systems have been established to identify CMM, i.e. ITS2 as a core barcode and psbA-trnH as a complementary locus for identification of planta medica, and COI as a core barcode and ITS2 as a complementary locus for identification of animal medica. This article introduced the principle of molecular identification of CMM using DNA barcoding and its drafting instructions. Furthermore, its application perspective was discussed.
authors.affiliation: The National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China. slchen@implad.ac.cn
authors.forename: Shi-Lin
authors.initials: SL
authors.lastname: Chen
authors.forename: Hui
authors.initials: H
authors.lastname: Yao
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Han
authors.forename: Tian-Yi
authors.initials: TY
authors.lastname: Xin
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Pang
authors.forename: Lin-Chun
authors.initials: LC
authors.lastname: Shi
authors.forename: Kun
authors.initials: K
authors.lastname: Luo
authors.forename: Jing-Yuan
authors.initials: JY
authors.lastname: Song
authors.forename: Dian-Yun
authors.initials: DY
authors.lastname: Hou
authors.forename: Shang-Mei
authors.initials: SM
authors.lastname: Shi
authors.forename: Zhong-Zhi
authors.initials: ZZ
authors.lastname: Qian
mesh_terms: D000818:Animals; D002681:China; D004247:DNA / Q000235:genetics; D058893:DNA Barcoding, Taxonomic / Q000379:methods*; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D004365:Drugs, Chinese Herbal / Q000145:classification* / Q000302:isolation & purification; D003576:Electron Transport Complex IV / Q000235:genetics; D008419:Materia Medica / Q000145:classification* / Q000302:isolation & purification; D008516:Medicine, Chinese Traditional; D010940:Plant Proteins / Q000235:genetics; D010946:Plants, Medicinal
chemical_list: D021903:DNA, Ribosomal Spacer; D004365:Drugs, Chinese Herbal; D008419:Materia Medica; D010940:Plant Proteins; D004247:DNA; D003576:Electron Transport Complex IV