title: Fast quantitative method for the analysis of carotenoids in transgenic maize.
abstract: A fast method was developed to determine carotenoid content in transgenic maize seeds. The analysis was carried out using an ultrahigh-pressure liquid chromatograph coupled to a photodiode array detector and a mass spectrometer (UHPLC-PDA-MS/MS). Sixteen carotenoid pigments were detected and quantified in <13 min. In addition, it was possible to obtain good resolution of both polar xanthophylls and nonpolar carotenes. The method exhibited (a) a high degree of repeatability (%RSD < 13%), (b) linear calibration curves (R² > 0.9952), (c) satisfactory recoveries for most of the pigments (between 82 and 108%), and (d) low detection (from 0.02 to 0.07 μg/mL) and quantification limits (from 0.05 to 0.20 μg/mL) (LOD and LOQ, respectively). The methodology was applied to the analysis of transgenic maize lines TM1, TM2, and TM3, expressing several carotenogenic genes.
authors.affiliation: Department of Chemistry, ETSEA, University of Lleida -Agrotecnio Center, Avenida Alcalde Rovira Roure 191, 25198 Lleida, Spain.
authors.forename: Sol Maiam
authors.initials: SM
authors.lastname: Rivera
authors.forename: Francisca
authors.initials: F
authors.lastname: Vilaró
authors.forename: Changfu
authors.initials: C
authors.lastname: Zhu
authors.forename: Chao
authors.initials: C
authors.lastname: Bai
authors.forename: Gemma
authors.initials: G
authors.lastname: Farré
authors.forename: Paul
authors.initials: P
authors.lastname: Christou
authors.forename: Ramon
authors.initials: R
authors.lastname: Canela-Garayoa
mesh_terms: D000818:Animals; D002338:Carotenoids / Q000032:analysis* / Q000096:biosynthesis / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D018556:Crops, Agricultural / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006801:Humans; D015394:Molecular Structure; D009753:Nutritive Value; D055668:Photochemical Processes; D030821:Plants, Genetically Modified / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry; D003313:Zea mays / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D002338:Carotenoids