title: Influence of the 524-VAAEIL-529 sequence of annexins A6 in their interfacial behavior and interaction with lipid monolayers.
abstract: Annexin A6 (AnxA6), a calcium- and membrane-binding protein, is expressed in mammalian cells in two isoforms: AnxA6-1 and AnxA6-2, the latter lacking the 524-VAAEIL-529 sequence at the start of repeat 7. The different intracellular localization of these two isoforms suggests distinct function in membrane dynamics. The aim of this work was to analyze the behavior of AnxA6 isoforms at the air/water interface alone and in the presence of membrane mimicking lipid monolayers. Using Langmuir technique showed that AnxA6-2 was less adsorbed to the neat air-water interface than AnxA6-1 at acidic pH and minor differences in their PM-IRRAS spectra were observed. Both isoforms exhibited similar behavior towards cholesterol monolayer. However, the interactions of AnxA6-2 with cholesterol ester monolayer were most favorable compared to AnxA6-1. Our experimental data are discussed in relation with the different intracellular localization of the two isoforms and with our constructed model of AnxA6-2 with the known crystal structure of AnxA6-1 showing the persistence of the 516-529 α-helix in AnxA6-2 despite the absence of the 524-VAAEIL-529 sequence.
authors.affiliation: Université de Lyon, Université Lyon 1, INSA de Lyon, CPE Lyon, ICBMS CNRS UMR 5246, Villeurbanne F-69622, France.
authors.forename: Magdalena M
authors.initials: MM
authors.lastname: Domon
authors.forename: Mehmet Nail
authors.initials: MN
authors.lastname: Nasir
authors.forename: Slawomir
authors.initials: S
authors.lastname: Pikula
authors.forename: Françoise
authors.initials: F
authors.lastname: Besson
mesh_terms: D000595:Amino Acid Sequence; D017317:Annexin A6 / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D008567:Membranes, Artificial; D008956:Models, Chemical; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D017317:Annexin A6; D008567:Membranes, Artificial; D020033:Protein Isoforms; D011994:Recombinant Proteins