title: Prognostic value of cyclin D1 expression in tumor-free surgical margins in head and neck squamous cell carcinomas.
abstract: CONCLUSIONS
  It was proved that cyclin D1-positive status in surgical margins was an independent prognostic indicator of local recurrence. The expression of cyclin D1 in tumor-free surgical margins may better predict local recurrence in patients with head and neck squamous cell carcinoma (HNSCC) after surgical treatment with curative intent.
  
  
  OBJECTIVE
  This retrospective study aimed to determine the prognostic indicators for local recurrence in HNSCC.
  
  
  METHODS
  A total of 116 HNSCC patients who underwent surgical treatment with curative intent and had histopathologically tumor-free margins were eligible for this study. The expression of p53 and cyclin D1 was assessed by immunohistochemical staining in surgical margins as well as in tumor specimens.
  
  
  RESULTS
  In all, 63 patients (54.3%) had p53-positive tumor specimens and 34 patients (29.3%) had p53-positive margins. Seventy-six patients (65.6%) had cyclin D1-positive tumor specimens and 54 patients (46.6%) had cyclin D1-positive margins. A significant difference in local control rates was observed between patients with cyclin D1-positive and -negative margins (77.2% vs 91.5%, log rank test, p = 0.0139). Multivariate Cox proportional hazards testing indicated that the hazard ratio of cyclin D1-positive margins for local recurrence was 4.58 (95% confidence interval 1.14-21.69, p = 0.0304).
authors.affiliation: Department of Otolaryngology- Head and Neck Surgery, Hokkaido University Graduate School of Medicine, Sapporo, Japan. t-sakashita@med.hokudai.ac.jp
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Sakashita
authors.forename: Akihiro
authors.initials: A
authors.lastname: Homma
authors.forename: Seigo
authors.initials: S
authors.lastname: Suzuki
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Hatakeyama
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kano
authors.forename: Takatsugu
authors.initials: T
authors.lastname: Mizumachi
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Oridate
authors.forename: Satoshi
authors.initials: S
authors.lastname: Fukuda
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000378:metabolism* / Q000401:mortality / Q000601:surgery; D019938:Cyclin D1 / Q000378:metabolism*; D005260:Female; D006258:Head and Neck Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000401:mortality / Q000601:surgery; D006801:Humans; D007564:Japan / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000175:diagnosis / Q000378:metabolism*; D011379:Prognosis; D012189:Retrospective Studies; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: C530104:CCND1 protein, human; D016159:Tumor Suppressor Protein p53; D019938:Cyclin D1