title: Characterization of x-type high-molecular-weight glutenin promoters (x-HGP) from different genomes in Triticeae.
abstract: The sequences of x-type high-molecular-weight glutenin promoter (x-HGP) from 21 diploid Triticeae species were cloned and sequenced. The lengths of x-HGP varied from 897 to 955 bp, and there are 329 variable sites including 105 singleton sites and 224 polymorphic sites. Genetic distances of pairwise X-HGP sequences ranged from 0.30 to 16.40% within 21 species and four outgroup species of Hordeum. All five recognized regulatory elements emerged and showed higher conservation in the x-HGP of 21 Triticeae species. Most variations were distributed in the regions among or between regulatory elements. A 22 bp and 50 bp insertions which were the copy of adjacent region with minor change, were found in the x-HGP of Ae. speltoides and Ps. Huashanica, and could be regarded as genome specific indels. The phylogeny of media-joining network and neighbour-joining tree both supported the topology were composed of three sperate clusters. Especially, the cluster I comprising the x-HGP sequences of Aegilops, Triticum, Henrardia, Agropyron and Taeniatherum was highly supporting by both network and NJ tree. As conferring to higher level and temporal and spatial expression, x-HGP can used as the source of promoter for constructing transgenic plants which allow endosperm-specific expression of exogenous gene on higher level. In addition, the x-HGP has enough conservation and variation; so it should be valuable in phylogenetic analyses of Triticeae family members.
authors.affiliation: Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130 China.
authors.forename: Qian-Tao
authors.initials: QT
authors.lastname: Jiang
authors.forename: Quan-Zhi
authors.initials: QZ
authors.lastname: Zhao
authors.forename: Xiu-Ying
authors.initials: XY
authors.lastname: Wang
authors.forename: Chang-Shui
authors.initials: CS
authors.lastname: Wang
authors.forename: Shan
authors.initials: S
authors.lastname: Zhao
authors.forename: Xue
authors.initials: X
authors.lastname: Cao
authors.forename: Xiu-Jin
authors.initials: XJ
authors.lastname: Lan
authors.forename: Zhen-Xiang
authors.initials: ZX
authors.lastname: Lu
authors.forename: You-Liang
authors.initials: YL
authors.lastname: Zheng
authors.forename: Yu-Ming
authors.initials: YM
authors.lastname: Wei
keywords: Evolution analysis; Regulatory element; Triticeae; x-type high-molecular-weight glutenin promoter (x-HGP)