title: BNIP3 mediates pre-myelinating oligodendrocyte cell death in hypoxia and ischemia.
abstract: Developing oligodendrocytes, collectively termed 'pre-myelinating oligodendrocytes' (preOLs), are vulnerable to hypoxic or ischemic insults. The underlying mechanism of this vulnerability remains unclear. Previously, we showed that Bcl-2⁄E1B-19K-interacting protein 3 (BNIP3), a proapoptotic member of the Bcl-2 family proteins, induced neuronal death in a caspase-independent manner in stroke. In this study, we investigated the role of BNIP3 in preOL cell death induced by hypoxia or ischemia. In primary oligodendrocyte progenitor cell (OPC) cultures exposed to oxygen-glucose deprivation, we found that BNIP3 was upregulated and levels of BNIP3 expression correlated with the death of OPCs. Up-regulation of BNIP3 was observed in preOLs in the white matter in a neonatal rat model of stroke. Knockout of BNIP3 significantly reduced death of preOLs in the middle cerebral artery occlusion model in mice. Our results demonstrate a role of BNIP3 in mediating preOLs cell death induced by hypoxia or ischemia, and suggest that BNIP3 may be a new target for protecting oligodendrocytes from death after stroke. Pre-myelinating oligodendrocytes (preOLs) are known to be highly vulnerable to ischemic insults. It remains unclear, however, how preOLs die. This study shows that BNIP3, a proapoptotic member of the Bcl-2 family proteins, is a mediator of hypoxia/ischemia-induced preOLs death. The BNIP3 cell death pathway may therefore be a new target for protecting oligodendrocytes from death after stroke.
authors.affiliation: Department of Histology and Embryology, Faculty of Basic Medicine, Third Military Medical University, Chongqing, China; Department of Human Anatomy and Cell Science, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Chengren
authors.initials: C
authors.lastname: Li
authors.forename: Teng
authors.initials: T
authors.lastname: Guan
authors.forename: Xueping
authors.initials: X
authors.lastname: Chen
authors.forename: Wenyan
authors.initials: W
authors.lastname: Li
authors.forename: Qiyan
authors.initials: Q
authors.lastname: Cai
authors.forename: Jianqin
authors.initials: J
authors.lastname: Niu
authors.forename: Lan
authors.initials: L
authors.lastname: Xiao
authors.forename: Jiming
authors.initials: J
authors.lastname: Kong
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002545:Brain Ischemia / Q000473:pathology*; D016923:Cell Death / Q000502:physiology*; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D005260:Female; D002534:Hypoxia, Brain / Q000473:pathology*; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D020244:Infarction, Middle Cerebral Artery / Q000473:pathology; D008297:Male; D008565:Membrane Proteins / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D024101:Mitochondrial Proteins / Q000502:physiology*; D009186:Myelin Sheath / Q000502:physiology; D009836:Oligodendroglia / Q000473:pathology* / Q000502:physiology*; D011518:Proto-Oncogene Proteins / Q000502:physiology*; D051381:Rats
chemical_list: C505650:BNIP3 protein, rat; C508686:BNip3 protein, mouse; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D011518:Proto-Oncogene Proteins
keywords: BNIP3; apoptosis; hypoxia; oligodendrocyte progenitor cell (OPC)