title: Solution structure and Rpn1 interaction of the UBL domain of human RNA polymerase II C-terminal domain phosphatase.
abstract: The ubiquitin-like modifier (UBL) domain of ubiquitin-like domain proteins (UDPs) interacts specifically with subunits of the 26 S proteasome. A novel UDP, ubiquitin-like domain-containing C-terminal domain phosphatase (UBLCP1), has been identified as an interacting partner of the 26 S proteasome. We determined the high-resolution solution structure of the UBL domain of human UBLCP1 by nuclear magnetic resonance spectroscopy. The UBL domain of hUBLCP1 has a unique β-strand (β3) and β3-α2 loop, instead of the canonical β4 observed in other UBL domains. The molecular topology and secondary structures are different from those of known UBL domains including that of fly UBLCP1. Data from backbone dynamics shows that the β3-α2 loop is relatively rigid although it might have intrinsic dynamic profile. The positively charged residues of the β3-α2 loop are involved in interacting with the C-terminal leucine-rich repeat-like domain of Rpn1.
authors.affiliation: Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul, Korea.
authors.forename: Ji-Hye
authors.initials: JH
authors.lastname: Yun
authors.forename: Sunggeon
authors.initials: S
authors.lastname: Ko
authors.forename: Chung-Kyung
authors.initials: CK
authors.lastname: Lee
authors.forename: Hae-Kap
authors.initials: HK
authors.lastname: Cheong
authors.forename: Chaejoon
authors.initials: C
authors.lastname: Cheong
authors.forename: Jong-Bok
authors.initials: JB
authors.lastname: Yoon
authors.forename: Weontae
authors.initials: W
authors.lastname: Lee
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000737:chemistry* / Q000378:metabolism*; D010749:Phosphoprotein Phosphatases / Q000737:chemistry* / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D012996:Solutions; D047992:TNF Receptor-Associated Factor 2; D047988:Tumor Necrosis Factor Receptor-Associated Peptides and Proteins / Q000378:metabolism*
chemical_list: D009687:Nuclear Proteins; C101338:PSMD2 protein, human; D012996:Solutions; D047992:TNF Receptor-Associated Factor 2; D047988:Tumor Necrosis Factor Receptor-Associated Peptides and Proteins; D010749:Phosphoprotein Phosphatases; C502029:UBLCP1 protein, human