title: Functionalization of nanostructured ZnO films by copper-free click reaction.
abstract: The copper-free click reaction was explored as a surface functionalization methodology for ZnO nanorod films grown by metal organic chemical vapor deposition (MOCVD). 11-Azidodecanoic acid was bound to ZnO nanorod films through the carboxylic acid moiety, leaving the azide group available for Cu-free click reaction with alkynes. The azide-functionalized layer was reacted with 1-ethynylpyrene, a fluorescent probe, and with alkynated biotin, a small biomolecule. The immobilization of pyrene on the surface was probed by fluorescence spectroscopy, and the immobilization of biotin was confirmed by binding with streptavidin-fluorescein isothiocyanate (streptavidin-FITC). The functionalized ZnO films were characterized by Fourier transform infrared attenuated total reflectance (FTIR-ATR), steady-state fluorescence emission, fluorescence microscopy, and field emission scanning electron microscopy (FESEM).
authors.affiliation: Department of Chemistry, Rutgers, the State University of New Jersey, 73 Warren Street, Newark, New Jersey 07102, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Cao
authors.forename: Elena
authors.initials: E
authors.lastname: Galoppini
authors.forename: Pavel Ivanoff
authors.initials: PI
authors.lastname: Reyes
authors.forename: Yicheng
authors.initials: Y
authors.lastname: Lu
mesh_terms: D001710:Biotin / Q000737:chemistry; D003652:Decanoic Acids / Q000737:chemistry; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D049329:Nanostructures / Q000737:chemistry*; D011721:Pyrenes / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D003652:Decanoic Acids; D008567:Membranes, Artificial; D011721:Pyrenes; C042881:1-ethynylpyrene; C031071:decanoic acid; D001710:Biotin; D015034:Zinc Oxide