title: Simultaneous Determination of Potassium Clavulanate and Amoxicillin Trihydrate in Bulk, Pharmaceutical Formulations and in Human Urine Samples by UV Spectrophotometry.
abstract: A simple and sensitive UV spectrophotometric method was developed and validated for the simultaneous determination of Potassium Clavulanate (PC) and Amoxicillin Trihydrate (AT) in bulk, pharmaceutical formulations and in human urine samples. The method was linear in the range of 0.2-8.5 μg/ml for PC and 6.4-33.6 μg/ml for AT. The absorbance was measured at 205 and 271 nm for PC and AT respectively. The method was validated with respect to accuracy, precision, specificity, ruggedness, robustness, limit of detection and limit of quantitation. This method was used successfully for the quality assessment of four PC and AT drug products and in human urine samples with good precision and accuracy. This is found to be simple, specific, precise, accurate, reproducible and low cost UV Spectrophotometric method.
authors.affiliation: Vardhman Life Sciences Pvt. Ltd., A-7, Sipcot Industrial Complex, Cuddalore (Tamilnadu), India.
authors.forename: Rajinder Singh
authors.initials: RS
authors.lastname: Gujral
authors.forename: Sk Manirul
authors.initials: SM
authors.lastname: Haque
keywords: amoxicillin trihydrate; bulk drug; human urine samples; pharmaceutical formulation; potassium clavulanate; spectrophotometer; uv