title: The 46359CT polymorphism of DNMT3B is associated with the risk of cervical cancer.
abstract: Abnormal methylation is related to cancer development. Since DNMT3B is an enzyme that modulates genomic methylation, we hypothesized that genetic variants of the promoter DNMT3B may be associated with an increased risk of developing cervical cancer. Our aim was to investigate the association between -579GT and 46359CT polymorphisms of DNMT3B and cervical cancer, high-grade squamous intraepithelial lesions (HSIL), and low-grade squamous intraepithelial lesions (LSIL). Samples from 200 healthy women and 130 women with squamous intraepithelial lesions (70 with cervical cancer, 30 with HSIL, and 30 with LSIL) were analyzed. Polymorphism genotyping was performed using PCR and restriction fragment length polymorphism. The -579GT polymorphism was not associated with cervical cancer, HSIL, or LSIL. The CT genotype of 46359CT polymorphism was significantly associated with cervical cancer risk (OR 8.75, CI 1.27-374.1), whereas the TT genotype was associated with a significantly decreased risk of HSIL (OR 0.66, CI 0.01-0.32) and LSIL (OR 0.11, CI 0.026-0.45). Our results suggest that genotyping the 46359CT polymorphism in DNMT3B may help identify women who are genetically susceptible to cervical cancer development. Additional studies with larger sample sizes are necessary to confirm our findings.
authors.affiliation: Laboratorio de Biomedicina Molecular, Unidad Académica de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero Chilpancingo, Guerrero Avenida Lázaro Cárdenas, C.P. 39090, Ciudad Universitaria, Chilpancingo, Guerrero, México.
authors.forename: Daniel
authors.initials: D
authors.lastname: Hernández-Sotelo
authors.forename: Rubén
authors.initials: R
authors.lastname: García-Aguilar
authors.forename: Yaneth
authors.initials: Y
authors.lastname: Castro-Coronel
authors.forename: Jonathan J
authors.initials: JJ
authors.lastname: Magaña
authors.forename: Marco Antonio
authors.initials: MA
authors.lastname: Leyva-Vazquez
authors.forename: Luz del Carmen
authors.initials: Ldel C
authors.lastname: Alarcón-Romero
authors.forename: Esther
authors.initials: E
authors.lastname: López-Bayghen
authors.forename: Berenice
authors.initials: B
authors.lastname: Illades-Aguiar
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D016022:Case-Control Studies; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D018307:Neoplasms, Squamous Cell / Q000235:genetics* / Q000473:pathology* / Q000821:virology; D011110:Polymorphism, Genetic; D012306:Risk; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000473:pathology* / Q000821:virology; D055815:Young Adult; D002578:Uterine Cervical Dysplasia / Q000235:genetics; D000097584:DNA Methyltransferase 3B
chemical_list: D004248:DNA (Cytosine-5-)-Methyltransferases; D000097584:DNA Methyltransferase 3B