title: Genome scan study of prostate cancer in Arabs: identification of three genomic regions with multiple prostate cancer susceptibility loci in Tunisians.
abstract: BACKGROUND
  Large databases focused on genetic susceptibility to prostate cancer have been accumulated from population studies of different ancestries, including Europeans and African-Americans. Arab populations, however, have been only rarely studied.
  
  
  METHODS
  Using Affymetrix Genome-Wide Human SNP Array 6, we conducted a genome-wide association study (GWAS) in which 534,781 single nucleotide polymorphisms (SNPs) were genotyped in 221 Tunisians (90 prostate cancer patients and 131 age-matched healthy controls). TaqMan SNP Genotyping Assays on 11 prostate cancer associated SNPs were performed in a distinct cohort of 337 individuals from Arab ancestry living in Qatar and Saudi Arabia (155 prostate cancer patients and 182 age-matched controls). In-silico expression quantitative trait locus (eQTL) analysis along with mRNA quantification of nearby genes was performed to identify loci potentially cis-regulated by the identified SNPs.
  
  
  RESULTS
  Three chromosomal regions, encompassing 14 SNPs, are significantly associated with prostate cancer risk in the Tunisian population (P = 1 × 10-4 to P = 1 × 10-5). In addition to SNPs located on chromosome 17q21, previously found associated with prostate cancer in Western populations, two novel chromosomal regions are revealed on chromosome 9p24 and 22q13. eQTL analysis and mRNA quantification indicate that the prostate cancer associated SNPs of chromosome 17 could enhance the expression of STAT5B gene.
  
  
  CONCLUSIONS
  Our findings, identifying novel GWAS prostate cancer susceptibility loci, indicate that prostate cancer genetic risk factors could be ethnic specific.
authors.affiliation: Laboratory of Genetic Medicine and Immunology, Weill Cornell Medical College in Qatar, Qatar Foundation, Doha, Qatar. loc2008@qatar-med.cornell.edu.
authors.forename: Jingxuan
authors.initials: J
authors.lastname: Shan
authors.forename: Khalid
authors.initials: K
authors.lastname: Al-Rumaihi
authors.forename: Danny
authors.initials: D
authors.lastname: Rabah
authors.forename: Issam
authors.initials: I
authors.lastname: Al-Bozom
authors.forename: Dhanya
authors.initials: D
authors.lastname: Kizhakayil
authors.forename: Karim
authors.initials: K
authors.lastname: Farhat
authors.forename: Sami
authors.initials: S
authors.lastname: Al-Said
authors.forename: Hala
authors.initials: H
authors.lastname: Kfoury
authors.forename: Shoba P
authors.initials: SP
authors.lastname: Dsouza
authors.forename: Jillian
authors.initials: J
authors.lastname: Rowe
authors.forename: Hanif G
authors.initials: HG
authors.lastname: Khalak
authors.forename: Shahzad
authors.initials: S
authors.lastname: Jafri
authors.forename: Idil I
authors.initials: II
authors.lastname: Aigha
authors.forename: Lotfi
authors.initials: L
authors.lastname: Chouchane
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D018912:Arabs / Q000235:genetics*; D016022:Case-Control Studies; D002874:Chromosome Mapping / Q000379:methods; D015331:Cohort Studies; D020022:Genetic Predisposition to Disease; D016678:Genome; D055106:Genome-Wide Association Study; D005838:Genotype; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide; D017430:Prostate-Specific Antigen / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000175:diagnosis* / Q000208:ethnology / Q000235:genetics*; D011780:Qatar; D040641:Quantitative Trait Loci; D012333:RNA, Messenger / Q000378:metabolism; D015203:Reproducibility of Results; D012307:Risk Factors; D012529:Saudi Arabia; D014416:Tunisia
chemical_list: D012333:RNA, Messenger; D017430:Prostate-Specific Antigen