title: Estimating the contribution of inhalation exposure to di-2-ethylhexyl phthalate (DEHP) for PVC production workers, using personal air sampling and urinary metabolite monitoring.
abstract: Because of troubling reports of high urinary metabolite levels and adverse reproductive health effects in workers exposed to di(2-ethylhexyl)phthalate (DEHP) in occupational settings, concern about exposure to DEHP in occupational settings is increasing. However, the contributions of different routes of exposure to DEHP are unclear. We used personal air sampling and biomonitoring to determine the contribution of inhalation exposure to the body burden of DEHP in the workplace. Eighty-nine workers (high-exposure group: 66 raw-materials workers; low-exposure group: 23 administrative workers) were recruited from three polyvinyl chloride (PVC) factories. Urinary levels of mono(2-ethylhexyl) phthalate (MEHP), (mono(2-ethyl-5-oxohexyl) phthalate (MEOHP), and mono(2-ethyl-5-hydroxyhexyl) phthalate (MEHHP) were measured in pre-shift and post-shift samples. The geometric means of airborne concentrations of DEHP were 5.3 μg/m3 (low-exposure group) and 32.7 μg/m3 (high-exposure group) (P<0.01). Correlation analysis showed a consistently significant association between airborne DEHP concentration and urinary DEHP metabolite levels in the high-exposure group. Calculating daily DEHP intake based on total urinary metabolite levels showed that the geometric means of total daily urinary metabolite levels of DEHP were 9.2 μg/kg/day (low-exposure group) and 15.5 μg/kg/day (high-exposure group) (P<0.01). A quartile analysis of all workers showed a significant trend toward an association between the individual contribution of inhalation exposure to DEHP and urinary DEHP metabolite levels, for which the mean inhalation contribution was 46.7% in the highest quartile. We conclude that inhalation-absorbed airborne DEHP significantly increased the total body burden of DEHP in these occupationally exposed workers.
authors.affiliation: Department of Environmental and Occupational Health, College of Medicine, National Cheng Kung University, 138 Sheng-Li Road, Tainan 704, Taiwan.
authors.forename: Jer-Pei
authors.initials: JP
authors.lastname: Fong
authors.forename: Fang-Jin
authors.initials: FJ
authors.lastname: Lee
authors.forename: I-Syuan
authors.initials: IS
authors.lastname: Lu
authors.forename: Shi-Nian
authors.initials: SN
authors.lastname: Uang
authors.forename: Ching-Chang
authors.initials: CC
authors.lastname: Lee
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000395:Air Pollutants, Occupational / Q000032:analysis*; D004051:Diethylhexyl Phthalate / Q000032:analysis*; D004784:Environmental Monitoring / Q000379:methods; D006801:Humans; D019570:Inhalation Exposure / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D016273:Occupational Exposure / Q000032:analysis*; D010968:Plasticizers / Q000032:analysis*; D011143:Polyvinyl Chloride; D055815:Young Adult
chemical_list: D000395:Air Pollutants, Occupational; D010968:Plasticizers; D011143:Polyvinyl Chloride; D004051:Diethylhexyl Phthalate
keywords: BMI; DEHP; Di(2-ethylhexyl) phthalate (DEHP); ESI; EU; European Union; Exposure contribution; GM; GSD; HPLC; LOD; MEHHP; MEHP; MEOHP; MS/MS; Occupational exposure; PVC; Polyvinyl chloride (PVC); RfD; SIP; SPE; U.S.; United States; body mass index; di(2-ethylhexyl) phthalate; electrospray ionization; geometric mean; geometric standard deviation; high performance liquid chromatography; limit of detection; mono(2-ethyl-5-hydroxyhexyl) phthalate; mono(2-ethyl-5-oxohexyl) phthalate; mono(2-ethylhexyl) phthalate; polyvinyl chloride; reference dose; solid phase extraction; summary index for plastic material contacted; tandem mass spectrometry