title: Improved HPLC purification strategy for [11C]raclopride and [11C]DASB leading to high radiochemical yields and more practical high quality radiopharmaceutical formulations.
abstract: A downscaled analytical HPLC purification strategy for [(11)C]raclopride and [(11)C]DASB was proposed to obtain a straightforward produced injectable solution with a substantially reduced volume. Both tracers were radiosynthesized using [(11)C]methyl triflate, and several analytical columns, in combination with ethanol containing eluents, were examined to optimize the chromatographic resolution. This resulted in a 5 mL solution of [(11)C]raclopride, obtained after 6 min, and a 7 mL solution of [(11)C]DASB, obtained after 10 min, using a C16-alkylamide and CN column respectively.
authors.affiliation: Laboratory for Radiopharmacy, Ghent University, Ghent, Belgium. nick.vanlaeken@Ugent.be
authors.forename: Nick
authors.initials: N
authors.lastname: Van Laeken
authors.forename: Ken
authors.initials: K
authors.lastname: Kersemans
authors.forename: Dieter
authors.initials: D
authors.lastname: De Meestere
authors.forename: Ingeborg
authors.initials: I
authors.lastname: Goethals
authors.forename: Filip
authors.initials: F
authors.lastname: De Vos
mesh_terms: D000814:Aniline Compounds / Q000138:chemical synthesis* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007553:Isotope Labeling / Q000379:methods*; D008422:Materials Testing; D020891:Raclopride / Q000138:chemical synthesis* / Q000302:isolation & purification*; D011829:Radiation Dosage; D019275:Radiopharmaceuticals / Q000138:chemical synthesis / Q000302:isolation & purification; D013440:Sulfides / Q000138:chemical synthesis* / Q000302:isolation & purification*
chemical_list: C412822:3-amino-4-(2-dimethylaminomethylphenylsulfanyl)benzonitrile; D000814:Aniline Compounds; D019275:Radiopharmaceuticals; D013440:Sulfides; D020891:Raclopride