title: Effects of allogenous periosteal-derived cells transfected with adenovirus-mediated BMP-2 on repairing defects of the mandible in rabbits.
abstract: OBJECTIVE
  This report describes the effect of periosteal-derived cells transfected with adenovirus-mediated bone morphogenetic protein-2 (BMP-2) on the repair of mandibular defects in rabbits.
  
  
  METHODS
  Periosteal-derived cells were transfected with a replication-defective adenoviral vector encoding BMP-2, and the expression of BMP-2 was examined in transfected cells using in situ hybridization and enzyme-linked immunosorbent assay. In addition, the proliferation ability and activity of alkaline phosphatase of transfected cells were examined using the 3-[4,5-dimethylthiazol-2-Yl]-2,5-diphenyltetrazolium bromide method and enzymology, respectively. In vitro critical-size defects (about 10 × 6 mm) were made bilaterally in each rabbit mandible, and individual sites were implanted with tissue-engineered bone modified with an adenovirus construct encoding the recombinant human BMP-2 gene (Ad-BMP-2), tissue-engineered bone without modification, single bioactive glass ceramic, or no implants (control). New bone formation was evaluated by histochemical stain.
  
  
  RESULTS
  BMP-2 expression in the supernate of infected cells was detected from the first day after Ad-BMP-2 transfection and remained at a high level for at least 2 weeks. Alkaline phosphatase expression in transfected cells was significantly greater than in uninfected cells. The group of Ad-BMP-2-modified periosteal-derived cells formed more new bone than the other group at any time point.
  
  
  CONCLUSIONS
  Gene-modified tissue-engineered bone grafts have greater osteogenic potential than single tissue-engineered bone and single bioactive glass ceramic graft. Ex vivo Ad-BMP-2 transfer to periosteal-derived cells can increase bone formation in critical-size bone defects. Further studies are needed to determine if modified engineered cells can be developed for safe and effective clinical applications.
authors.affiliation: Attending Doctor, Department of Stomatology, QiLu Hospital of Shandong University, Jinan, Shandong, China.
authors.forename: Mingxia
authors.initials: M
authors.lastname: Sun
authors.forename: Wanye
authors.initials: W
authors.lastname: Tan
authors.forename: Ketao
authors.initials: K
authors.lastname: Wang
authors.forename: Zuoqing
authors.initials: Z
authors.lastname: Dong
authors.forename: Huahai
authors.initials: H
authors.lastname: Peng
authors.forename: Fengcai
authors.initials: F
authors.lastname: Wei
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000469:Alkaline Phosphatase / Q000032:analysis; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000627:therapeutic use*; D001861:Bone Regeneration / Q000235:genetics; D018786:Bone Substitutes / Q000737:chemistry; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D017690:Cell Transplantation; D002516:Ceramics / Q000737:chemistry; D004396:Coloring Agents; D004797:Enzyme-Linked Immunosorbent Assay; D005822:Genetic Vectors / Q000235:genetics*; D048091:Guided Tissue Regeneration / Q000379:methods; D006801:Humans; D017403:In Situ Hybridization; D008336:Mandibular Diseases / Q000601:surgery*; D010012:Osteogenesis / Q000502:physiology; D010521:Periosteum / Q000166:cytology*; D011817:Rabbits; D011994:Recombinant Proteins / Q000627:therapeutic use; D013778:Tetrazolium Salts; D013844:Thiazoles; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014162:Transfection; D016212:Transforming Growth Factor beta / Q000627:therapeutic use*; D014184:Transplantation, Homologous
chemical_list: D055396:Bone Morphogenetic Protein 2; D018786:Bone Substitutes; D004396:Coloring Agents; D011994:Recombinant Proteins; D013778:Tetrazolium Salts; D013844:Thiazoles; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; C023506:Glass ceramics; D000469:Alkaline Phosphatase; C022616:thiazolyl blue