title: Application of supercritical CO2 for extraction of polyisoprenoid alcohols and their esters from plant tissues.
abstract: In this study, a method of supercritical fluid extraction (SFE) with carbon dioxide of polyisoprenoids from plant photosynthetic tissues is described. SFE was an effective extraction method for short- and medium-chain compounds with even higher yield than that observed for the "classical extraction" method with organic solvents. Moreover, SFE-derived extracts contained lower amounts of impurities (e.g., chlorophylls) than those obtained by extraction of the same tissue with organic solvents. Elevated temperature and extended extraction time of SFE resulted in a higher rate of extraction of long-chain polyisoprenoids. Ethanol cofeeding did not increase the extraction efficiency of polyisoprenoids; instead, it increased the content of impurities in the lipid extract. Optimization of SFE time and temperature gives the opportunity of prefractionation of complex polyisoprenoid mixtures accumulated in plant tissues. Extracts obtained with application of SFE are very stable and free from organic solvents and can further be used directly in experimental diet supplementation or as starting material for preparation of semisynthetic polyisoprenoid derivatives, e.g., polyisoprenoid phosphates.
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
authors.forename: Adam
authors.initials: A
authors.lastname: Jozwiak
authors.forename: Robert
authors.initials: R
authors.lastname: Brzozowski
authors.forename: Zygmunt
authors.initials: Z
authors.lastname: Bujnowski
authors.forename: Tadeusz
authors.initials: T
authors.lastname: Chojnacki
authors.forename: Ewa
authors.initials: E
authors.lastname: Swiezewska
mesh_terms: D000438:Alcohols / Q000737:chemistry / Q000302:isolation & purification*; D002245:Carbon Dioxide / Q000737:chemistry*; D025924:Chromatography, Supercritical Fluid; D004952:Esters / Q000737:chemistry / Q000302:isolation & purification*; D028222:Picea / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry / Q000302:isolation & purification*; D031994:Sorbus / Q000737:chemistry; D013696:Temperature; D013997:Time Factors; D014026:Nicotiana / Q000737:chemistry
chemical_list: D000438:Alcohols; D004952:Esters; D011108:Polymers; D002245:Carbon Dioxide
keywords: Nicotiana tabacum; Picea abies; Sorbus intermedia; polyprenol; solanesol; supercritical fluid extraction