title: The role of preameloblast-conditioned medium in dental pulp regeneration.
abstract: Pulp regeneration using human dental pulp stem cells (hDPSCs) maintains tooth vitality compared with conventional root canal therapy. Our previous study demonstrated that preameloblast-conditioned medium (PA-CM) from murine apical bud cells induces the odontogenic differentiation of hDPSCs and promoted dentin formation in mouse subcutaneous tissue. The purpose of the present study is to evaluate the effects of PA-CM with human whole pulp cells on pulp regeneration in an empty root canal space. Human pulp cells were seeded in the pulp cavities of 5 mm-thick human tooth segments with or without PA-CM treatment, and then transplanted subcutaneously into immunocompromised mice. In the pulp cell-only group, skeletal muscle with pulp-like tissue was generated in the pulp cavity. A reparative dentin-like structure with entrapped cells lined the existing dentin wall. However, in the PA-CM-treated group, only pulp-like tissue was regenerated without muscle or a reparative dentin-like structure. Moreover, human odontoblast-like cells exhibited palisade arrangement around the pulp, and typical odontoblast processes elongated into dentinal tubules. The results suggest that PA-CM can induce pulp regeneration of human pulp cells with physiological structures in an empty root canal space.
authors.affiliation: Department of Oral Histology-Developmental Biology and Dental Research Institute, School of Dentistry, Seoul National University, 28 Yeongun-dong, Chongro-gu, Seoul, 110-749, Korea.
authors.forename: Han-Wool
authors.initials: HW
authors.lastname: Choung
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Lee
authors.forename: Dong-Seol
authors.initials: DS
authors.lastname: Lee
authors.forename: Pill-Hoon
authors.initials: PH
authors.lastname: Choung
authors.forename: Joo-Cheol
authors.initials: JC
authors.lastname: Park
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism; D017077:Culture Media, Conditioned; D003782:Dental Pulp / Q000166:cytology / Q000502:physiology*; D048091:Guided Tissue Regeneration; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D051379:Mice; D009804:Odontoblasts / Q000378:metabolism / Q000648:ultrastructure; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000378:metabolism* / Q000648:ultrastructure; D055815:Young Adult
chemical_list: D000954:Antigens, Surface; D017077:Culture Media, Conditioned; D018960:Hyaluronan Receptors; C525591:STRO-1 antigen, human