title: T helper and regulatory T cell cytokine profile in active, stable and narrow band ultraviolet B treated generalized vitiligo.
abstract: BACKGROUND
  Vitiligo is a T cell mediated autoimmune depigmenting disease. Altered cytokine concentrations have been suggested in the pathogenesis of vitiligo.
  
  
  METHODS
  T helper and regulatory T cell cytokines (IL-2, IFN-γ, IL-10, IL-13, IL-17 and TGF-β) have been estimated by ELISA and their clinical correlation was determined. The study had 3 groups: group I with 80 vitiligo patients (60 active and 20 stable), group II with 25 narrow band ultraviolet B treated vitiligo and group III with 70 healthy controls.
  
  
  RESULTS
  Significant difference was found in the serum interleukin (IL)-10, IL-13, IL-17A and TGF-β1 concentrations among 3 groups (P<0.05). In group I, serum IL-2, IL-17A concentrations were significantly increased and TGF-β1 concentrations were decreased in active vitiligo compared to stable vitiligo (P<0.05). Concentrations of IL-2, IL-10 and IL-13 (rho=-0.307, rho=-0.407, rho=-0.351 and P<0.05; respectively) were negatively- and TGF-β1 concentrations were positively-correlated (rho=0.799, P=0.001) with disease duration. Interleukin-13 concentrations were negatively- and serum TGF-β1 concentrations were positively-correlated (rho=-0.326, rho=0.244 and P<0.05; respectively) with percentage of body surface area involvement.
  
  
  CONCLUSIONS
  Increased concentrations of serum IL-10, IL-13, and IL-17A and decreased concentrations of TGF-β1 suggested altered cell-mediated immunity that may facilitate the melanocyte cytotoxicity in vitiligo.
authors.affiliation: Department of Dermatology & Venereology, AIIMS, New Delhi, India.
authors.forename: Manoj Kumar
authors.initials: MK
authors.lastname: Tembhre
authors.forename: Vinod Kumar
authors.initials: VK
authors.lastname: Sharma
authors.forename: Alpana
authors.initials: A
authors.lastname: Sharma
authors.forename: Parthaprasad
authors.initials: P
authors.lastname: Chattopadhyay
authors.forename: Somesh
authors.initials: S
authors.lastname: Gupta
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D016207:Cytokines / Q000097:blood* / Q000276:immunology; D005260:Female; D006801:Humans; D007111:Immunity, Cellular / Q000528:radiation effects; D008297:Male; D008544:Melanocytes / Q000276:immunology / Q000378:metabolism* / Q000528:radiation effects; D012720:Severity of Illness Index; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology / Q000378:metabolism* / Q000528:radiation effects; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000378:metabolism* / Q000528:radiation effects; D014466:Ultraviolet Rays; D014820:Vitiligo / Q000097:blood* / Q000276:immunology / Q000473:pathology / Q000532:radiotherapy
chemical_list: D016207:Cytokines
keywords: Active/stable generalized vitiligo; Cytokines; GV; IFN; IL; NB-UVB; Narrowband ultraviolet B (NB-UVB); Regulatory T cells; T helper; T helper cells; TGF; TNF; Th; Treg; generalized vitiligo; interferon; interleukin; narrowband ultra violet B; regulatory T cell; transforming growth factor; tumor necrosis factor