title: EBV-miR-BART1 is involved in regulating metabolism-associated genes in nasopharyngeal carcinoma.
abstract: EBV-miR-BART1 has been found to be highly expressed in some cancers including nasopharyngeal carcinoma (NPC), but its exact roles in the pathogenesis of NPC remain unclear. Here, we did RNA deep sequencing to compare the gene expression profile between EBV-miR-BART1-expressing CNE1 cells and the control cells to determine the possible effects of EBV-miR-BART1 in NPC. Gene expression profiling analysis unexpectedly showed a significant number of up- and down-modulated metabolism-associated genes, such as G6PD, SAT1, ASS1, PAST1, FUT1, SGPL1, DHRS3, B4GALT1, PHGDH, IDH2, PISD, UGT8, LDHB and GALNT1, in EBV-miR-BART1-expressing NPC cells, which were next confirmed by RT-qPCR. Moreover, of these metabolism-genes, PSAT1 and PHGDH expression levels were significantly upregulated and most of other genes were obviously up-expressed in NPC specimens compared with chronic nasopharyngitis (CNP) tissues. Collectively, we for the first time found the effects of EBV-miR-BART1 on the expression of mechanism-associated genes in NPC, suggesting a novel role of EBV-miR-BART1 in cancer metabolism, which remains to be fully elucidated.
authors.affiliation: Cancer Research Institute and the Provincial Key Laboratory of Functional Proteomics, Southern Medical University, Guangzhou 510515, PR China.
authors.forename: Yanfen
authors.initials: Y
authors.lastname: Ye
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.forename: Lan
authors.initials: L
authors.lastname: Zhang
authors.forename: Yuxiang
authors.initials: Y
authors.lastname: Chen
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Lyu
authors.forename: Longmei
authors.initials: L
authors.lastname: Cai
authors.forename: Yaoyong
authors.initials: Y
authors.lastname: Lu
authors.forename: Yi
authors.initials: Y
authors.lastname: Deng
authors.forename: Jianguo
authors.initials: J
authors.lastname: Wang
authors.forename: Kaitai
authors.initials: K
authors.lastname: Yao
authors.forename: Weiyi
authors.initials: W
authors.lastname: Fang
authors.forename: Hongbing
authors.initials: H
authors.lastname: Cai
authors.forename: Xin
authors.initials: X
authors.lastname: Li
mesh_terms: D001706:Biopsy; D002277:Carcinoma; D002352:Carrier Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D004279:DNA, Viral / Q000032:analysis; D020031:Epstein-Barr Virus Infections / Q000378:metabolism; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D004854:Herpesvirus 4, Human / Q000378:metabolism*; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism* / Q000821:virology; D009304:Nasopharyngitis / Q000378:metabolism; D014157:Transcription Factors
chemical_list: C485676:ARL2BP protein, human; D002352:Carrier Proteins; D004279:DNA, Viral; D035683:MicroRNAs; D014157:Transcription Factors