title: Nasal delivery of PLG microparticle encapsulated defensin peptides adjuvanted gp41 antigen confers strong and long-lasting immunoprotective response against HIV-1.
abstract: Defensins display immunostimulatory activities including a chemotactic effect for T lymphocytes/immature dendritic cells and secretion of pro-inflammatory cytokines suggest their role in bridging innate and adaptive immunity. We hypothesized whether defensins with separately emulsified HIV-1 immunogen would elicit peptide-specific systemic and mucosal antibody response in mice. The HIV-1 peptide alone in microsphere showed low peptide-specific antibody response in sera and different washes, while the presence of defensins markedly increased the antibody peak titre both in sera (102,400-409,600) (p < 0.05) and in washes (800-25,600) (p < 0.001). Defensins with HIV-1 peptide were showing 43.0-83.2% and 38.7-72.3% in vitro neutralization against laboratory isolates in serum and lavage samples, respectively, higher than HIV-1 peptide alone. Our findings may have implications in the development of new mucosal adjuvant for AIDS vaccination.
authors.affiliation: Department of Biochemistry, All India Institute of Medical Sciences (A.I.I.M.S.), Room Number 3029, New Delhi, 110029, India, mohan.teena@gmail.com.
authors.forename: Teena
authors.initials: T
authors.lastname: Mohan
authors.forename: D
authors.initials: D
authors.lastname: Mitra
authors.forename: D N
authors.initials: DN
authors.lastname: Rao
mesh_terms: D016915:AIDS Vaccines / Q000008:administration & dosage* / Q000276:immunology; D000281:Administration, Intranasal; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D023082:Defensins / Q000008:administration & dosage* / Q000276:immunology; D005260:Female; D015700:HIV Envelope Protein gp41 / Q000008:administration & dosage* / Q000276:immunology; D015658:HIV Infections / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D015497:HIV-1 / Q000276:immunology*; D018928:Immunity, Mucosal / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008863:Microspheres; D009500:Neutralization Tests; D018709:Statistics, Nonparametric
chemical_list: D016915:AIDS Vaccines; D000914:Antibodies, Viral; D023082:Defensins; D015700:HIV Envelope Protein gp41; C514787:gp41 protein, Human immunodeficiency virus 1