title: Transmembrane protein 208: a novel ER-localized protein that regulates autophagy and ER stress.
abstract: Autophagy and endoplasmic reticulum (ER) stress are both tightly regulated cellular processes that play central roles in various physiological and pathological conditions. Recent reports have indicated that ER stress is a potent inducer of autophagy. However, little is known about the underlying molecular link between the two processes. Here we report a novel human protein, transmembrane protein 208 (TMEM208) that can regulate both autophagy and ER stress. When overexpressed, TMEM208 impaired autophagy as characterized by the decrease of the accumulation of LC3-II, decreased degradation of autophagic substrates, and reduced expression of critical effectors and vital molecules of the ER stress and autophagy processes. In contrast, knockdown of the TMEM208 gene promoted autophagy, as demonstrated by the increase of LC3-II, increased degradation of autophagic substrates, and enhanced expression levels for genes key in the ER stress and autophagic processes. Taken together, our results reveal that this novel ER-located protein regulates both ER stress and autophagy, and represents a possible link between the two different cellular processes.
authors.affiliation: Key Laboratory of Medical Immunology, Ministry of Health, Peking University Health Science Center, Beijing, China.
authors.forename: Yuanbo
authors.initials: Y
authors.lastname: Zhao
authors.forename: Jia
authors.initials: J
authors.lastname: Hu
authors.forename: Guangyan
authors.initials: G
authors.lastname: Miao
authors.forename: Liujing
authors.initials: L
authors.lastname: Qu
authors.forename: Zhenda
authors.initials: Z
authors.lastname: Wang
authors.forename: Ge
authors.initials: G
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Lv
authors.forename: Dalong
authors.initials: D
authors.lastname: Ma
authors.forename: Yingyu
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D001343:Autophagy; D001483:Base Sequence; D004721:Endoplasmic Reticulum / Q000378:metabolism*; D059865:Endoplasmic Reticulum Stress; D005786:Gene Expression Regulation; D006358:Hot Temperature; D006801:Humans; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D021381:Protein Transport
chemical_list: D008565:Membrane Proteins; C585265:TMEM208 protein, human