title: Conditioned serum-free medium from umbilical cord mesenchymal stem cells has anti-photoaging properties.
abstract: Chronic exposure to solar radiation is the primary cause of photoaging and benign and malignant skin tumors. A conditioned serum-free medium (SFM) was prepared from umbilical cord mesenchymal stem cells (UC-MSCs) and its anti-photoaging effect, following chronic UV irradiation in vitro and in vivo, was evaluated. UC-MSC SFM had a stimulatory effect on human dermal fibroblast proliferation and reduced UVA-induced cell death. In addition, UC-MSC SFM blocked UVA inhibition of superoxide dismutase activity. Topical application of UC-MSC SFM to mouse skin prior to UV irradiation blocked the inhibition of superoxide dismutase and glutathione peroxidase activities, and prevented the upregulation of malonaldehyde. UC-MSC SFM thus protects against photoaging induced by UVA and UVB radiation and is a promising candidate for skin anti-photoaging treatments.
authors.affiliation: Biomedical R&D Center, Life and Science Technology College, Jinan University, 5th Floor, 601 Huangpu Road, Guangzhou, 510632, People's Republic of China.
authors.forename: Qiuying
authors.initials: Q
authors.lastname: Liu
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Luo
authors.forename: Shaoqing
authors.initials: S
authors.lastname: He
authors.forename: Xinlei
authors.initials: X
authors.lastname: Peng
authors.forename: Sheng
authors.initials: S
authors.lastname: Xiong
authors.forename: Yifei
authors.initials: Y
authors.lastname: Wang
authors.forename: Xueyun
authors.initials: X
authors.lastname: Zhong
authors.forename: Xiangrong
authors.initials: X
authors.lastname: Zhou
authors.forename: Carol A
authors.initials: CA
authors.lastname: Eisenberg
authors.forename: Bruce Z
authors.initials: BZ
authors.lastname: Gao
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002470:Cell Survival / Q000528:radiation effects*; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000737:chemistry*; D016895:Culture Media, Serum-Free / Q000737:chemistry; D005347:Fibroblasts / Q000502:physiology / Q000528:radiation effects*; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D008315:Malondialdehyde / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000378:metabolism* / Q000502:physiology*; D051379:Mice; D012867:Skin / Q000201:enzymology / Q000378:metabolism / Q000528:radiation effects; D013482:Superoxide Dismutase / Q000378:metabolism; D014466:Ultraviolet Rays; D014470:Umbilical Cord / Q000166:cytology*
chemical_list: D017077:Culture Media, Conditioned; D016895:Culture Media, Serum-Free; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase