title: Α-tocopherol prevents ERK1/2 activation in PC12 cells under conditions of oxidative stress and its contribution to the protective effect.
abstract: Preincubation with 100 nM and 100 μM α-tocopherol for 18 h prevented long-term activation of extracellular signal-activated kinase (ERK1/2), induced by H2O2in PC12 cells. α-Tocopherol significantly reduced H2O2-induced death of PC12 cell, but its protective effect was significantly lower in the presence of ERK1/2 inhibitor. These data show that prevention of long-term activation of ERK1/2 by α-tocopherol contributes to the increase in viability of PC12 cells exposed to H2O2. This fact suggests that inhibition of ERK1/2 activity by α-tocopherol reduces neuronal cell death in the brain under conditions of oxidative stress in vivo.
authors.affiliation: Laboratory of Comparative Neurochemistry, I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Saint-Petersburg, Russia.
authors.forename: I O
authors.initials: IO
authors.lastname: Zakharova
authors.forename: T V
authors.initials: TV
authors.lastname: Sokolova
authors.forename: N F
authors.initials: NF
authors.lastname: Avrova
mesh_terms: D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D004789:Enzyme Activation; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D016716:PC12 Cells; D051381:Rats; D024502:alpha-Tocopherol / Q000494:pharmacology*
chemical_list: D006861:Hydrogen Peroxide; D048049:Extracellular Signal-Regulated MAP Kinases; D024502:alpha-Tocopherol