title: A DNA-binding protein encoded by ORF008L of Singapore grouper iridovirus.
abstract: Singapore grouper iridovirus (SGIV) is a major viral pathogen that can cause substantial economic losses in aquaculture, but its genome replication, organization and package are largely unknown. We isolated SGIV protein-DNA core by freeze-thaw lysis of viral particles and gradient centrifugation. Twelve proteins were identified from the core by mass spectrometry. ORF008L, one of the core proteins, was identified as a collagen-like protein and its DNA binding ability was demonstrated by electrophoretic mobility shift assay (EMSA). Binding of ORF008L to DNA was neither sequence specific nor pH dependent, and it protected DNA from degradation by DNase I in vitro. These results suggest that ORF008L may play a role in protection or stabilization of the viral genome during infection.
authors.affiliation: Mechanobiology Institute, National University of Singapore, Singapore 117411, Singapore.
authors.forename: Lili
authors.initials: L
authors.lastname: Wang
authors.forename: Qing Yun
authors.initials: QY
authors.lastname: Chong
authors.forename: Jinlu
authors.initials: J
authors.lastname: Wu
mesh_terms: D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D024202:Electrophoretic Mobility Shift Assay; D011485:Protein Binding; D017995:Ranavirus / Q000235:genetics / Q000502:physiology*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D014764:Viral Proteins
keywords: Collagen; DMTase; DNA methyltransferase; DNA-binding protein; EMSA; GE cells; SGIV; Singapore grouper iridovirus; electrophoretic mobility shift assay; grouper embryonic cells