title: An ultrasensitive electrochemiluminescence immunoassay for carbohydrate antigen 19-9 in serum based on antibody labeled Fe3O4 nanoparticles as capture probes and graphene/CdTe quantum dot bionanoconjugates as signal amplifiers.
abstract: The CdTe quantum dots (QDs), graphene nanocomposite (CdTe-G) and dextran-Fe3O4 magnetic nanoparticles have been synthesized for developing an ultrasensitive electrochemiluminescence (ECL) immunoassay for Carcinoembryonic antigen 19-9 (CA 19-9) in serums. Firstly, the capture probes (CA 19-9 Ab1/Fe3O4) for enriching CA 19-9 were synthesized by immobilizing the CA 19-9's first antibody (CA 19-9 Ab1) on magnetic nanoparticles (dextran-Fe3O4). Secondly, the signal probes (CA 19-9 Ab2/CdTe-G), which can emit an ECL signal, were formed by attaching the secondary CA 19-9 antibody (CA 19-9 Ab2) to the surface of the CdTe-G. Thirdly, the above two probes were used for conjugating with a serial of CA 19-9 concentrations. Graphene can immobilize dozens of CdTe QDs on their surface, which can emit stronger ECL intensity than CdTe QDs. Based on the amplified signal, ultrasensitive antigen detection can be realized. Under the optimal conditions, the ECL signal depended linearly on the logarithm of CA 19-9 concentration from 0.005 to 100 pg/mL, and the detection limit was 0.002 pg/mL. Finally, five samples of human serum were tested, and the results were compared with a time-resolved fluorescence assay (TRFA). The novel immunoassay provides a stable, specific and highly sensitive immunoassay protocol for tumor marker detection at very low levels, which can be applied in early diagnosis of tumor.
authors.affiliation: The State Key Laboratory Base of Novel Functional Materials and Preparation Science, School of Material Science and Chemical Engineering, Ningbo University, Ningbo 315211, China. ganning@nbu.edu.cn.
authors.forename: Ning
authors.initials: N
authors.lastname: Gan
authors.forename: Jing
authors.initials: J
authors.lastname: Zhou
authors.forename: Ping
authors.initials: P
authors.lastname: Xiong
authors.forename: Tianhua
authors.initials: T
authors.lastname: Li
authors.forename: Shan
authors.initials: S
authors.lastname: Jiang
authors.forename: Yuting
authors.initials: Y
authors.lastname: Cao
authors.forename: Qianli
authors.initials: Q
authors.lastname: Jiang
mesh_terms: D000906:Antibodies / Q000737:chemistry; D018395:CA-19-9 Antigen / Q000097:blood*; D019187:Cadmium Compounds / Q000737:chemistry; D017097:Electric Impedance; D055664:Electrochemical Techniques / Q000379:methods; D006108:Graphite / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008163:Luminescent Measurements / Q000379:methods; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D045663:Quantum Dots; D015203:Reproducibility of Results; D013691:Tellurium / Q000737:chemistry
chemical_list: D000906:Antibodies; D018395:CA-19-9 Antigen; D019187:Cadmium Compounds; D058185:Magnetite Nanoparticles; D006108:Graphite; D013691:Tellurium