title: Generation of murine sympathoadrenergic progenitor-like cells from embryonic stem cells and postnatal adrenal glands.
abstract: Sympathoadrenergic progenitor cells (SAPs) of the peripheral nervous system (PNS) are important for normal development of the sympathetic PNS and for the genesis of neuroblastoma, the most common and often lethal extracranial solid tumor in childhood. However, it remains difficult to isolate sufficient numbers of SAPs for investigations. We therefore set out to improve generation of SAPs by using two complementary approaches, differentiation from murine embryonic stem cells (ESCs) and isolation from postnatal murine adrenal glands. We provide evidence that selecting for GD2 expression enriches for ESC-derived SAP-like cells and that proliferating SAP-like cells can be isolated from postnatal adrenal glands of mice. These advances may facilitate investigations about the development and malignant transformation of the sympathetic PNS.
authors.affiliation: Department of Pediatrics and Adolescent Medicine, University Medical Center Ulm, Ulm, Germany.
authors.forename: Shobhit
authors.initials: S
authors.lastname: Saxena
authors.forename: Joachim
authors.initials: J
authors.lastname: Wahl
authors.forename: Markus S
authors.initials: MS
authors.lastname: Huber-Lang
authors.forename: Dominic
authors.initials: D
authors.lastname: Stadel
authors.forename: Peter
authors.initials: P
authors.lastname: Braubach
authors.forename: Klaus-Michael
authors.initials: KM
authors.lastname: Debatin
authors.forename: Christian
authors.initials: C
authors.lastname: Beltinger
mesh_terms: D000222:Adaptation, Physiological; D000311:Adrenal Glands / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D018959:CD57 Antigens / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D015870:Gene Expression; D051379:Mice; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D013564:Sympathetic Nervous System / Q000166:cytology* / Q000378:metabolism
chemical_list: D015415:Biomarkers; D018959:CD57 Antigens