title: Generation and properties of antibacterial coatings based on electrostatic attachment of silver nanoparticles to protein-coated polypropylene fibers.
abstract: We present a simple method for attaching silver nanoparticles to polypropylene (PP) fibers in a two-step process to impart antibacterial properties. Specifically, PP fibers are pretreated by the adsorption from an aqueous solution of heat-denatured lysozyme (LYS) followed by LYS cross-linking using glutaraldehyde and sodium borohydride. At neutral pH, the surface of the adsorbed LYS layer is enriched with numerous positive charges. Silver nanoparticles (AgNPs) capped with trisodium citrate are subsequently deposited onto the protein-coated PP. Nanoparticle binding is mediated by electrostatic interactions between the positively charged LYS layer and the negatively charged AgNPs. The density of AgNPs deposited on PP depends on the amount of protein adsorbed on the surface. UV-vis spectroscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, and scanning electron microscopy are employed to follow all preparation steps and to characterize the resulting functional surfaces. The antibacterial activity of the modified surfaces is tested against gram negative bacteria Escherichia coli (E. coli). Overall, our results show that PP surfaces coated with AgNPs exhibit excellent antibacterial activity with 100% removal efficiency.
authors.affiliation: Department of Materials Science & Engineering, North Carolina State University, Raleigh, North Carolina 27695-7907, United States.
authors.forename: Kiran K
authors.initials: KK
authors.lastname: Goli
authors.forename: Nimish
authors.initials: N
authors.lastname: Gera
authors.forename: Xiaomeng
authors.initials: X
authors.lastname: Liu
authors.forename: Balaji M
authors.initials: BM
authors.lastname: Rao
authors.forename: Orlando J
authors.initials: OJ
authors.lastname: Rojas
authors.forename: Jan
authors.initials: J
authors.lastname: Genzer
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects*; D004927:Escherichia coli Infections / Q000517:prevention & control; D057927:Hydrophobic and Hydrophilic Interactions; D008027:Light; D053768:Metal Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D011126:Polypropylenes / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry; D012834:Silver / Q000737:chemistry*; D055672:Static Electricity; D014466:Ultraviolet Rays
chemical_list: D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D011126:Polypropylenes; D011506:Proteins; D012834:Silver