title: Lactobacillus decelerates cervical epithelial cell cycle progression.
abstract: We investigated cell cycle progression in epithelial cervical ME-180 cells during colonization of three different Lactobacillus species utilizing live cell microscopy, bromodeoxyuridine incorporation assays, and flow cytometry. The colonization of these ME-180 cells by L. rhamnosus and L. reuteri, originating from human gastric epithelia and saliva, respectively, was shown to reduce cell cycle progression and to cause host cells to accumulate in the G1 phase of the cell cycle. The G1 phase accumulation in L. rhamnosus-colonized cells was accompanied by the up-regulation and nuclear accumulation of p21. By contrast, the vaginal isolate L. crispatus did not affect cell cycle progression. Furthermore, both the supernatants from the lactic acid-producing L. rhamnosus colonies and lactic acid added to cell culture media were able to reduce the proliferation of ME-180 cells. In this study, we reveal the diversity of the Lactobacillus species to affect host cell cycle progression and demonstrate that L. rhamnosus and L. reuteri exert anti-proliferative effects on human cervical carcinoma cells.
authors.affiliation: Department of Molecular Biosciences, The Wenner-Grens Institute, Stockholm University, Stockholm, Sweden.
authors.forename: Katarina
authors.initials: K
authors.lastname: Vielfort
authors.forename: Linda
authors.initials: L
authors.lastname: Weyler
authors.forename: Niklas
authors.initials: N
authors.lastname: Söderholm
authors.forename: Mattias
authors.initials: M
authors.lastname: Engelbrecht
authors.forename: Sonja
authors.initials: S
authors.lastname: Löfmark
authors.forename: Helena
authors.initials: H
authors.lastname: Aro
mesh_terms: D002453:Cell Cycle / Q000502:physiology*; D002455:Cell Division; D002460:Cell Line; D049109:Cell Proliferation; D002584:Cervix Uteri / Q000166:cytology* / Q000382:microbiology*; D048749:Cytokinesis; D004847:Epithelial Cells / Q000378:metabolism* / Q000382:microbiology; D005260:Female; D016193:G1 Phase; D006801:Humans; D019344:Lactic Acid / Q000096:biosynthesis; D007778:Lactobacillus / Q000502:physiology*
chemical_list: D019344:Lactic Acid