title: Simple boric acid-based fluorescent focusing for sensing of glucose and glycoprotein via multipath moving supramolecular boundary electrophoresis chip.
abstract: Boric acid-based fluorescent complex probe of BBV-HPTS (boronic acid-based benzyl viologen (BBV) and hydroxypyrene trisulfonic acid trisodium salt (HPTS)) was rarely used for sensitive sensing of saccharide (especially glycoprotein) via electrophoresis. We proposed a novel model of moving supramolecular boundary (MSB) formed with monosaccharide or glycoprotein in microcolumn and the complex probe of BBV-HPTS in the cathodic injection tube, developed a method of MSB fluorescent focusing for sensitive recognition of monosaccharide and glycoprotein, and designed a special multipath capillary electrophoresis (CE) chip for relative experiments. As a proof of concept, glucose and hemoglobin A1c (HbA1c) were respectively used as the mode saccharide and glycoprotein for the relevant demonstration. The experiments revealed that (i) the complex of BBV-HPTS could interact with free glucose or bound one in glycoprotein; (ii) the fluorescent signal was a function of glucose or glycoprotein content approximately; and (iii) interestingly the fluorescent band motion was dependent on glucose content. The developed method had the following merits: (i) low cost; (ii) low limit of detection (down to 1.39 pg/mL for glucose and 2.0 pg per capillary HbA1c); and (iii) high throughput (up to 12 runs or more per patch) and speed (less than 5 min). The developed method has potential use for sensitive monitoring of monosaccharide and glycoprotein in biomedical samples.
authors.affiliation: Laboratory of Analytical Biochemistry and Bioseparation, State Key Laboratory of Microbial Metabolism, and School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Jingyu
authors.initials: J
authors.lastname: Dong
authors.forename: Si
authors.initials: S
authors.lastname: Li
authors.forename: Houyu
authors.initials: H
authors.lastname: Wang
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Meng
authors.forename: Liuyin
authors.initials: L
authors.lastname: Fan
authors.forename: Haiyang
authors.initials: H
authors.lastname: Xie
authors.forename: Chengxi
authors.initials: C
authors.lastname: Cao
authors.forename: Weibing
authors.initials: W
authors.lastname: Zhang
mesh_terms: D001888:Boric Acids / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D005947:Glucose / Q000032:analysis*; D006023:Glycoproteins / Q000032:analysis* / Q000097:blood; D006801:Humans; D040081:Protein Array Analysis / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D001888:Boric Acids; D006023:Glycoproteins; D005947:Glucose; C032688:boric acid