title: Endurance training enhances LXRα gene expression in Wistar male rats.
abstract: Liver X receptor α (LXRα) is a member of the ligand-activated transcription factor of nuclear hormonal receptor superfamily, whose activation leads to modulation in the expression of genes involved in cholesterol homeostasis including ATP-binding cassette transporter A1 (ABCA1), which plays a crucial role in plasma high-density lipoprotein cholesterol (HDL-C) remodeling. The purpose of this study was to investigate whether endurance training enhanced the expression level of liver LXRα gene. Twelve adult male Wistar rats (200-220 g) were divided into control and training groups. Training group received exercise on a motor-driven treadmill at 28 m/min (0 % grade) for 60 min/day, 5 days/week for 8 weeks. Twenty-four hours after the last exercise session, the rats were killed and blood was taken from the right ventricle of each rat. Plasma was collected for HDL-C, low-density lipoprotein cholesterol (LDL-C), TC and TG measurements. Furthermore, a portion of the liver of each rat was excised and washed in ice-cold saline and frozen in liquid nitrogen for assessment of LXRα and ABCA1 mRNA levels. Data indicated significant increase in both LXRα and ABCA1 mRNA levels in trained rats, compared to control rats. Plasma HDL-C concentration was significantly higher (P < 0.001) in trained rats at the end of treadmill exercise. However, there was a significant decrease in LDL-C (P < 0.003), TG, TC concentration, TC/HDL-C and LDL/HDL-C ratios in trained rats compared with those in the control group (P < 0.001). In conclusion, we found that endurance training induced significant elevation in LXRα gene expression, which correlated with enhanced levels of ABCA1 mRNA and plasma HDL-C concentration.
authors.affiliation: Department of Exercise Physiology, Faculty of Sport Sciences, University of Isfahan, Isfahan, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Kazeminasab
authors.forename: Mohammad
authors.initials: M
authors.lastname: Marandi
authors.forename: Kamran
authors.initials: K
authors.lastname: Ghaedi
authors.forename: Fahimeh
authors.initials: F
authors.lastname: Esfarjani
authors.forename: Jamal
authors.initials: J
authors.lastname: Moshtaghian
mesh_terms: D064286:ATP Binding Cassette Transporter 1 / Q000235:genetics; D000818:Animals; D002784:Cholesterol / Q000097:blood / Q000235:genetics; D008076:Cholesterol, HDL / Q000097:blood / Q000235:genetics; D008078:Cholesterol, LDL / Q000097:blood / Q000235:genetics; D015870:Gene Expression / Q000235:genetics* / Q000502:physiology; D008099:Liver / Q000378:metabolism; D000071518:Liver X Receptors; D008297:Male; D057093:Orphan Nuclear Receptors / Q000235:genetics*; D010807:Physical Endurance / Q000235:genetics* / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017208:Rats, Wistar; D014280:Triglycerides / Q000097:blood / Q000235:genetics
chemical_list: D064286:ATP Binding Cassette Transporter 1; D002784:Cholesterol; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D000071518:Liver X Receptors; D057093:Orphan Nuclear Receptors; D012333:RNA, Messenger; D014280:Triglycerides; C577834:Abca1 protein, rat; C000605215:Nr1h3 protein, rat