title: Isolation of infectious chikungunya virus and dengue virus using anionic polymer-coated magnetic beads.
abstract: Mosquitoes-borne viruses are a major threat for human populations. Among them, chikungunya virus (CHIKV) and dengue virus (DENV) cause thousands of cases worldwide. The recent propagation of mosquito vectors competent to transmit these viruses to temperate areas increases their potential impact on susceptible human populations. The development of sensitive methods allowing the detection and isolation of infectious viruses is of crucial interest for determination of virus contamination in humans and in competent mosquito vectors. However, simple and rapid method allowing the capture of infectious CHIKV and DENV from samples with low viral titers useful for further genetic and functional characterization of circulating strains is lacking. The present study reports a fast and sensitive isolation technique based on viral particles adsorption on magnetic beads coated with anionic polymer, poly(methyl vinyl ether-maleic anhydrate) and suitable for isolation of infectious CHIKV and DENV from the four serotypes. Starting from quite reduced biological material, this method was accurate to combine with conventional detection techniques, including qRT-PCR and immunoblotting and allowed isolation of infectious particles without resorting to a step of cultivation. The use of polymer-coated magnetic beads is therefore of high interest for rapid detection and isolation of CHIKV and DENV from samples with reduced viral loads and represents an accurate approach for the surveillance of mosquito vector in area at risk for arbovirus outbreaks.
authors.affiliation: Laboratoire MIVEGEC, UMR 224 IRD/CNRS/UM1, Montpellier, France.
authors.forename: Sirilaksana
authors.initials: S
authors.lastname: Patramool
authors.forename: Eric
authors.initials: E
authors.lastname: Bernard
authors.forename: Rodolphe
authors.initials: R
authors.lastname: Hamel
authors.forename: Luplertlop
authors.initials: L
authors.lastname: Natthanej
authors.forename: Nathalie
authors.initials: N
authors.lastname: Chazal
authors.forename: Pornapat
authors.initials: P
authors.lastname: Surasombatpattana
authors.forename: Peeraya
authors.initials: P
authors.lastname: Ekchariyawat
authors.forename: Simon
authors.initials: S
authors.lastname: Daoust
authors.forename: Supatra
authors.initials: S
authors.lastname: Thongrungkiat
authors.forename: Frédéric
authors.initials: F
authors.lastname: Thomas
authors.forename: Laurence
authors.initials: L
authors.lastname: Briant
authors.forename: Dorothée
authors.initials: D
authors.lastname: Missé
mesh_terms: D018354:Alphavirus Infections / Q000175:diagnosis / Q000821:virology*; D000818:Animals; D002646:Chikungunya virus / Q000302:isolation & purification*; D009033:Culicidae / Q000821:virology*; D003715:Dengue / Q000175:diagnosis / Q000821:virology*; D003716:Dengue Virus / Q000302:isolation & purification*; D006801:Humans; D008280:Magnetics; D008863:Microspheres; D011108:Polymers; D012680:Sensitivity and Specificity; D013048:Specimen Handling / Q000379:methods; D013997:Time Factors; D014773:Virology / Q000379:methods*
chemical_list: D011108:Polymers
keywords: Arbovirus; Chikungunya; Dengue; Magnetic beads; Viro-Adembeads