title: Cloning and characterization of bottlenose dolphin (Tursiops truncatus) interleukin-10.
abstract: The function of cytokines in cetaceans has so far only been determined for the proinflammatory cytokines. In this study, we cloned bottlenose dolphin (Tursiops truncatus) interleukin-10 (IL-10) cDNA from concanavalin A (Con A)-stimulated peripheral blood mononuclear cells (PBMC), and investigated the mRNA expression levels in various tissues and the bioactivity of recombinant dolphin (rd) IL-10. The gene encodes a polypeptide of 178 amino acids which encompasses the mature protein sequence of 158 amino acids. Quantitative expression analysis of dolphin IL-10 revealed that the highest mRNA levels are found in the spleen. To assess its function, rdIL-10 was produced in human embryonic kidney 293 cells and its bioactivity was demonstrated through IL-10-induced inhibition of proinflammatory cytokine mRNA expression IFN-γ, TNF-α, and IL-2 of Con A-stimulated PBMC. These results indicated that the structure and function of bottlenose dolphin IL-10 is similar to that of other animals. This is the first report of the characterization of an anti-inflammatory cytokine in cetaceans.
authors.affiliation: Nihon University Veterinary Research Center, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Segawa
authors.forename: Nana
authors.initials: N
authors.lastname: Karatani
authors.forename: Takuya
authors.initials: T
authors.lastname: Itou
authors.forename: Miwa
authors.initials: M
authors.lastname: Suzuki
authors.forename: Takeo
authors.initials: T
authors.lastname: Sakai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D050681:Bottle-Nosed Dolphin / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D018076:DNA, Complementary; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger; D016753:Interleukin-10