title: Exploring the use of five color flow cytometry to examine the effect of acute tai chi practice on pro inflammatory monocyte subtypes - biomed 2013.
abstract: OBJECTIVE
  The purpose of this study was to determine whether five-color flow cytometry could be used to examine the effect of acute Tai Chi (TC) practice on pro-inflammatory monocyte subtypes within the total monocyte population in peripheral blood.
  
  
  METHODS
  A pilot pre- post- intervention design was our experimental model: pre-intervention measurements acted as the control for the post- intervention measurements. The research was conducted at Landsbaum Center for Health Education in a Midwestern community. Healthy subjects were asked to perform their style of TC for one hour.
  
  
  METHODS
  Four milliliter of peripheral blood was collected in the BD Vacutainer CPT tubes immediately prior and after one hour of TC practice. Peripheral blood mononuclear cells (PBMCs) were isolated following the manufacturers protocol. PBMCs were then assessed for CD14, CD16, TLR2, TLR4, and HLA-DR expressions using five-color flow cytometry measurement. FlowJo and Graphpad Prism software were used to analyze the results.
  
  
  RESULTS
  The proportion of pro-inflammatory CD14+CD16+ monocytes decreased immediately after one hour of TC practice (p<0.01).
  
  
  CONCLUSIONS
  The results indicate that the five-color flow cytometric process was sufficiently sensitive to be used to explore changes in peripheral blood pro-inflammatory monocytes following one hour of TC practice.
authors.affiliation: Indiana University School of Medicine.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Li
authors.forename: Roy W
authors.initials: RW
authors.lastname: Geib