title: Enhancing diffractive multi-plane microscopy using colored illumination.
abstract: We present a method to increase the number of simultaneously imaged focal planes in diffractive multi-plane imaging. We exploit the chromatic properties of diffraction by using multicolor LED illumination and demonstrate time-synchronous imaging of up to 21 focal planes.We discuss the possibilities and limits given by the use of a liquid crystal spatial light modulator to display the diffractive patterns. The method is suitable for wide-field transmission and reflection microscopy.
authors.affiliation: Division of Biomedical Physics, Innsbruck Medical University, M¨ullerstraße 44, 6020 Innsbruck, Austria. alexander.jesacher@i-med.ac.at
authors.forename: Alexander
authors.initials: A
authors.lastname: Jesacher
authors.forename: Clemens
authors.initials: C
authors.lastname: Roider
authors.forename: Monika
authors.initials: M
authors.lastname: Ritsch-Marte
mesh_terms: D003124:Colorimetry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D008029:Lighting / Q000295:instrumentation*; D008853:Microscopy / Q000379:methods*; D012031:Refractometry / Q000295:instrumentation*