title: Tissue and swab culture in diabetic foot infections: neuropathic versus neuroischemic ulcers.
abstract: We evaluated the diagnostic performance of swabs versus tissue cultures in 28 diabetic patients with neuropathic (group A) and 22 diabetic patients with neuroischemic foot ulcer (group B) and the differences in bacterial isolates between the 2 groups. In group A, sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of swab cultures for the diagnosis of infection were 100%, 40%, 88.5%, and 100%, respectively. In group B, the corresponding values were 100%, 22.2%, 65%, and 100%. In group A, sensitivity, specificity, PPV, and NPV of swab cultures for the identification of pathogens were 100%, 14.3%, 53.8%, and 100%, respectively. In group B, the corresponding values were 100%, 18.2%, 55%, and 100%. In each group, Staphylococcus aureus and Pseudomonas aeruginosa were the most common isolates. The number of isolates was significantly higher on swab versus tissue cultures only in group A (P = .033). No differences were observed between groups in number of isolates and colony forming units. In conclusion, swab cultures are highly sensitive but less specific and have an excellent NPV both in diabetic patients with neuropathic and in those with neuroischemic foot ulcer. There are no differences between the groups in microbial load.
authors.affiliation: Democritus University of Thrace, University Hospital of Alexandroupolis, Greece. maria_thdemetriou@yahoo.gr
authors.forename: Maria
authors.initials: M
authors.lastname: Demetriou
authors.forename: Nikolaos
authors.initials: N
authors.lastname: Papanas
authors.forename: Maria
authors.initials: M
authors.lastname: Panopoulou
authors.forename: Konstantinos
authors.initials: K
authors.lastname: Papatheodorou
authors.forename: Anastasios
authors.initials: A
authors.lastname: Bounovas
authors.forename: Efstratios
authors.initials: E
authors.lastname: Maltezos
mesh_terms: D001706:Biopsy / Q000379:methods; D002478:Cells, Cultured; D015169:Colony Count, Microbial; D003925:Diabetic Angiopathies / Q000382:microbiology / Q000473:pathology; D017719:Diabetic Foot / Q000382:microbiology / Q000473:pathology*; D003929:Diabetic Neuropathies / Q000382:microbiology / Q000473:pathology; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity; D014946:Wound Infection / Q000382:microbiology / Q000473:pathology*
keywords: culture; diabetes mellitus; diabetic foot; diagnosis; infection; ischemia; neuropathy