title: Regulators of human adipose-derived stem cell self-renewal.
abstract: Adipose tissue is an alternative source of mesenchymal stem cells and human adipose-derived stem cells (ASCs) display an attractive and substantial therapeutic potential when transplanted in animal models. To this end, an understanding of ASC biology is necessary and the knowledge of mechanisms that maintain ASCs in an undifferentiated state with no loss of differentiation potential during ex vivo expansion represents a crucial step. However, these mechanisms remain to be identified because appropriate human cellular models are scant. In this review we will describe a cellular model isolated from human adipose tissue displaying all the features of stem cells. Then, we will focus on the identification of intrinsic and extrinsic factors regulating the balance between human ASC proliferation and differentiation. We will point out the role of factors secreted by undifferentiated ASCs, such a FGF2, activin A, BMP4, Hedgehog molecules and secreted by adipose tissue macrophages. Finally, we will outline the role of miRNAs in these processes.
authors.affiliation: Université de Nice Sophia-Antipolis, Institut Biologie du Développement et Cancer, CNRS UMR 6543, Faculté de Médecine 28 avenue de Valombrose, 06108 Nice Cedex 2, France.
authors.forename: Phi
authors.initials: P
authors.lastname: Villageois
authors.forename: Brigitte
authors.initials: B
authors.lastname: Wdziekonski
authors.forename: Laure-Emmanuelle
authors.initials: LE
authors.lastname: Zaragosi
authors.forename: Magali
authors.initials: M
authors.lastname: Plaisant
authors.forename: Tala
authors.initials: T
authors.lastname: Mohsen-Kanson
authors.forename: Nadège
authors.initials: N
authors.lastname: Lay
authors.forename: Annie
authors.initials: A
authors.lastname: Ladoux
authors.forename: Pascal
authors.initials: P
authors.lastname: Peraldi
authors.forename: Christian
authors.initials: C
authors.lastname: Dani
keywords: Adipose-derived stem cells; FGF2; Hedgehog; activin A; adipocyte differentiation; miR30; proliferation