title: Growth factor receptor-bound protein 14: a potential new gene associated with oocyte competence.
abstract: The Grb14 protein is a member of the Grb7 protein family. This protein family acts by binding to tyrosine kinase receptors, promoting cell proliferation and differentiation. There is evidence of the involvement of tyrosine kinase factors in the bovine oocyte maturation process. However, Grb14 has not been studied for bovine cumulus-oocyte complexes (COCs). The aim of the present study was to characterize Grb14 mRNA expression in bovine COCs during follicular development. Furthermore, we demonstrated that the expression of Grb14 mRNA is not regulated by estradiol. mRNA expression of Grb14 was assessed in 480 COCs from follicles of different sizes (1-3, 4-6, 6-8 or >8 mm) by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Grb14 mRNA expression decreased in COCs throughout follicular growth (P < 0.05). The role of estradiol in the expression of Grb14 mRNA in COCs was studied. Grb14 mRNA abundance did not differ in COCs cultured in the presence or absence of 17β-estradiol or fulvestrant. In conclusion, we showed that Grb14 mRNA is downregulated in COCs during antral follicle development, a finding that suggests a role for Grb14 in oocyte competence.
authors.affiliation: Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil.
authors.forename: Paulo Roberto Antunes
authors.initials: PR
authors.lastname: Rosa
authors.affiliation: Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil.
authors.forename: Rodrigo Camponogara
authors.initials: RC
authors.lastname: Bohrer
authors.affiliation: Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil.
authors.forename: Matheus Pedrotti
authors.initials: MP
authors.lastname: De Cesaro
authors.affiliation: Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil.
authors.forename: Karina
authors.initials: K
authors.lastname: Gutierrez
authors.affiliation: Department of Animal Science, Santa Catarina State University, Chapecó, SC, 89802-200, Brazil.
authors.forename: Rogério
authors.initials: R
authors.lastname: Ferreira
authors.affiliation: Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil.
authors.forename: Gabriel Ribas
authors.initials: GR
authors.lastname: Pereira
authors.affiliation: Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil.
authors.forename: João Francisco Coelho
authors.initials: JF
authors.lastname: Oliveira
authors.affiliation: Laboratory of Biotechnology and Animal Reproduction- BioRep, Federal University of Santa Maria, Av. Roraima #1000, CEP 97105-900, Santa Maria, RS 97105-900, Brazil.
authors.forename: Paulo Bayard Dias
authors.initials: PB
authors.lastname: Gonçalves
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D000818:Animals; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D054885:Cumulus Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D004958:Estradiol / Q000494:pharmacology; D004967:Estrogens / Q000494:pharmacology; D005260:Female; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D009866:Oogenesis / Q000187:drug effects / Q000502:physiology; D006080:Ovarian Follicle / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D004967:Estrogens; D012333:RNA, Messenger; D004958:Estradiol