title: [Effect of blocking endogenous miR-23a on the proliferation and invasion in gastric adenocarcinoma cell line MGC803].
abstract: OBJECTIVE
  To investigate the effect of functional blocking of endogenous miR-23a with a specific antisense oligonucleotide (ASO) on the proliferation and invasiveness of gastric adenocarcinoma cell line MGC803 in vitro.
  
  
  METHODS
  A specific ASO targeting miR-23a, namely ASO-23a, was transfected into MGC803 cells to block endogenous miR-23a. The mRNA level of miR-23a in the transfected cells was detected with quantitative real-time PCR. The changes of cell proliferation following the transfection were detected with MTT assay and colony formation assay, and TUNEL assay and Transwell assay were employed to evaluate the changes in cell apoptosis and invasiveness, respectively.
  
  
  RESULTS
  Quantitative real-time PCR demonstrated efficient functional blocking of endogenous miR-23a in MGC803 cells by ASO-23a. Suppression of miR-23a with ASO-23a obviously inhibited cell growth, colony formation and invasiveness of MGC803 cells and significantly enhanced the cell apoptosis.
  
  
  CONCLUSIONS
  ASO-23a can efficiently block the function of endogenous miR-23a in MGC803 cells to inhibit cell proliferation and invasion and promote cell apoptosis.
authors.affiliation: College of Basic Medicine, Hebei United University, Tangshan, China. zhulihua1972@163.com
authors.forename: Lihua
authors.initials: L
authors.lastname: Zhu
authors.forename: Jiali
authors.initials: J
authors.lastname: Tian
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.forename: Meimei
authors.initials: M
authors.lastname: Wang
authors.forename: Yanan
authors.initials: Y
authors.lastname: Xiong
authors.forename: Guangling
authors.initials: G
authors.lastname: Zhang
authors.forename: Shuying
authors.initials: S
authors.lastname: Li
authors.forename: Lijie
authors.initials: L
authors.lastname: Yuan
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology; D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D016376:Oligonucleotides, Antisense; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D014162:Transfection
chemical_list: C552430:MIRN23a microRNA, human; D035683:MicroRNAs; D016376:Oligonucleotides, Antisense