title: Analysis of adult neurogenesis: evidence for a prominent "non-neurogenic" DCX-protein pool in rodent brain.
abstract: Here, we have developed a highly sensitive immunoassay for Dcx to characterize expression in brain and cerebrospinal fluid (CSF) of rodents. We demonstrate that Dcx is widely expressed during development in various brain regions and as well can be detected in cerebrospinal fluid of rats (up to 30 days postnatal). While Dcx protein level decline in adulthood and were detectable in neurogenic regions of the adult rodent brain, similar levels were also detectable in brain regions expected to bear no neurogenesis including the cerebral cortex and CA1/CA3 enriched hippocampus. We monitored DCX protein levels after paradigms to increase or severely decrease adult hippocampal neurogenesis, namely physical activity and cranial radiation, respectively. In both paradigms, Dcx protein- and mRNA-levels clearly reflected changes in neurogenesis in the hippocampus. However, basal Dcx-levels are unaffected in non-neurogenic regions (e.g. CA1/CA3 enriched hippocampus, cortex). These data suggest that there is a substantial "non-neurogenic" pool of Dcx- protein, whose regulation can be uncoupled from adult neurogenesis suggesting caution for the interpretation of such studies.
authors.affiliation: F. Hoffmann-La Roche AG, Pharma Research & Early Development, DTA Neuroscience, Basel, Switzerland. Thomas.kremer@roche.com
authors.forename: Thomas
authors.initials: T
authors.lastname: Kremer
authors.forename: Ravi
authors.initials: R
authors.lastname: Jagasia
authors.forename: Annika
authors.initials: A
authors.lastname: Herrmann
authors.forename: Hugues
authors.initials: H
authors.lastname: Matile
authors.forename: Edilio
authors.initials: E
authors.lastname: Borroni
authors.forename: Fiona
authors.initials: F
authors.lastname: Francis
authors.forename: Hans Georg
authors.initials: HG
authors.lastname: Kuhn
authors.forename: Christian
authors.initials: C
authors.lastname: Czech
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D005260:Female; D005786:Gene Expression Regulation / Q000528:radiation effects; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects; D008297:Male; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000134:cerebrospinal fluid / Q000235:genetics / Q000378:metabolism*; D055495:Neurogenesis / Q000528:radiation effects; D009479:Neuropeptides / Q000134:cerebrospinal fluid / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats
chemical_list: C000714847:Dcx protein, mouse; C000714848:Dcx protein, rat; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D008869:Microtubule-Associated Proteins; D009479:Neuropeptides; D012333:RNA, Messenger