title: Phosphatidylinositol synthase activity from plant membrane fractions and from E. coli-expressed recombinant peptides.
abstract: Phosphatidylinositol (PtdIns) synthase is a lipid-synthesizing enzyme responsible for the synthesis of the phospholipid, PtdIns. Its enzymatic properties have been studied in in vitro assays using either membrane-enriched fractions or the purified protein in reconstituted lipid vesicles as a source of enzyme. More recently the specificities in terms of substrate preferences have also been studied using the recombinant protein expressed in Escherichia coli. This chapter deals with the purification of membranes as a source of PtdIns synthase before focusing on the in vitro assays of the enzymatic activities of the protein and, briefly, on the analysis of the product.
authors.affiliation: Physiologie Cellulaire et Moléculaire des Plantes, Université Pierre et Marie Curie, EAC 7180 CNRS, Paris, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Collin
authors.forename: Françoise
authors.initials: F
authors.lastname: Cochet
mesh_terms: D017360:Arabidopsis / Q000201:enzymology; D051607:CDP-Diacylglycerol-Inositol 3-Phosphatidyltransferase / Q000378:metabolism*; D002462:Cell Membrane / Q000201:enzymology*; D057075:Enzyme Assays / Q000379:methods*; D004926:Escherichia coli / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism*; D013347:Subcellular Fractions / Q000201:enzymology
chemical_list: D010455:Peptides; D011994:Recombinant Proteins; D051607:CDP-Diacylglycerol-Inositol 3-Phosphatidyltransferase