title: NF-κB activity is inversely correlated to RNF11 expression in Parkinson's disease.
abstract: RING finger protein 11 (RNF11), a negative regulator of NF-κB signaling pathway, colocalizes with α-synuclein and is sequestered in Lewy bodies in Parkinson's disease (PD). Since persistent NF-κB activation is reported in PD, in this report we investigated if RNF11 expression level is correlated to activated NF-κB in PD. We examined RNF11 expression levels in correlation to phospho-p65, a marker for activated NF-κB, in control and PD brain tissue from cerebral cortex. In addition we performed double immunofluorescence labeling experiments to confirm this correlation. Our investigations demonstrated that the neuronal RNF11 expression was down-regulated in PD and was usually associated with increased expression of phospho-p65. Double labeling confirmed that loss of neuronal RNF11 was linked to increased phospho-p65 expression, suggesting that persistent presence of NF-κB activation could be due to decreased levels of its negative regulator. Our data exemplifies the relevance of RNF11 and persistent NF-κB activation in PD.
authors.affiliation: Department of Neurology, Emory University, Atlanta, GA, USA.
authors.forename: Elaine
authors.initials: E
authors.lastname: Pranski
authors.forename: Carson D
authors.initials: CD
authors.lastname: Van Sanford
authors.forename: Nirjari
authors.initials: N
authors.lastname: Dalal
authors.forename: Adam L
authors.initials: AL
authors.lastname: Orr
authors.forename: Dipan
authors.initials: D
authors.lastname: Karmali
authors.forename: Deborah S
authors.initials: DS
authors.lastname: Cooper
authors.forename: Marla
authors.initials: M
authors.lastname: Gearing
authors.forename: James J
authors.initials: JJ
authors.lastname: Lah
authors.forename: Allan I
authors.initials: AI
authors.lastname: Levey
authors.forename: Ranjita
authors.initials: R
authors.lastname: Betarbet
mesh_terms: D001921:Brain / Q000378:metabolism*; D002352:Carrier Proteins / Q000032:analysis / Q000378:metabolism*; D004268:DNA-Binding Proteins; D005455:Fluorescent Antibody Technique; D006801:Humans; D016328:NF-kappa B / Q000032:analysis / Q000378:metabolism*; D010300:Parkinson Disease / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D016328:NF-kappa B; C404508:RNF11 protein, human