title: Single-molecule microscopy of the green fluorescent protein using simultaneous two-color excitation.
abstract: In vivo microscopy of the Green Fluorescent Protein (GFP), the most important label in cell biology, with single-molecule sensitivity is hampered by an insufficient signal-to-noise ratio. A significant improvement is obtained with a novel two-color excitation technique. The picture clearly shows the increased brightness of GFP in in vitro single-molecule assays and in live-cell microscopy under two-color illumination (upper cell) as compared to normal illumination (lower cell).
authors.affiliation: Department Chemie, Lehrstuhl Physikalische Chemie I, Ludwig-Maximilians Universität München, München, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Jung
authors.forename: J
authors.initials: J
authors.lastname: Wiehler
authors.forename: B
authors.initials: B
authors.lastname: Steipe
authors.forename: C
authors.initials: C
authors.lastname: Bräuchle
authors.forename: A
authors.initials: A
authors.lastname: Zumbusch
mesh_terms: D003116:Color; D049452:Green Fluorescent Proteins / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
chemical_list: D049452:Green Fluorescent Proteins