title: Genomic analysis of bacteriophage PBECO4 infecting Escherichia coli O157:H7.
abstract: Escherichia coli O157:H7 is a human pathogen. We isolated a novel bacteriophage infecting this bacterium from a sewage water treatment facility. Phage PBECO4 belongs to the family Myoviridae, having an isometric head and a contractile tail. It has a linear double-stranded DNA genome of 348,113 base pairs in length with a GC content of 34.09 %. Whole-genome sequencing revealed that PBECO4 is distantly related to enterobacteria phage vB_KleM_RaK2, with 10 % similarity, and Cronobacter phage vB_CsaM_GAP32 with 6 % similarity. Five hundred fifty-one putative open reading frames (ORFs) and six tRNA genes were found. Eight ORFs are related to genes encoding structural proteins, nine to DNA packaging, two to DNA lysis activity, and 42 to replication and regulation. Four hundred ninety ORFs have not been functionally annotated.
authors.affiliation: Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, Yongin, Korea.
authors.forename: Min Soo
authors.initials: MS
authors.lastname: Kim
authors.forename: Sung Sik
authors.initials: SS
authors.lastname: Hong
authors.forename: Kwangseo
authors.initials: K
authors.lastname: Park
authors.forename: Heejoon
authors.initials: H
authors.lastname: Myung
mesh_terms: D003090:Coliphages / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D019453:Escherichia coli O157 / Q000821:virology*; D016679:Genome, Viral / Q000235:genetics*; D023281:Genomics; D006801:Humans; D008969:Molecular Sequence Data; D017900:Myoviridae / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D012343:RNA, Transfer / Q000235:genetics; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins; D012343:RNA, Transfer