title: Is the pre-Tg DSC endotherm observed with solid state proteins associated with the protein internal dynamics? Investigation of bovine serum albumin by solid state hydrogen/deuterium exchange.
abstract: DSC thermograms of solid state pure proteins often show a distinct endotherm at a temperature far below the glass transition temperature of the system (Tg). We hypothesized this endotherm represents enthalpy recovery associated with an internal mobility transition of the protein molecule. Although the existence of an internal transition has been postulated, whether this endotherm is associated with such a transition has not previously been discussed. The purpose of this study was to investigate the origin of the pre-Tg endotherm in lyophilized bovine serum albumin (BSA). Due to strong glass behavior, the system Tg was determined by extrapolating Tg data of disaccharide/BSA formulations to zero saccharide. A small pre-Tg endotherm around 40-60 °C was observed in amorphous BSA equilibrated at 11%RH. The apparent activation energy suggested the endotherm was "α-mobility"-related. A solid state hydrogen/deuterium exchange study using FTIR was conducted over a temperature range spanning the endotherm. We found a fast phase, followed by essentially a plateau level which is highly temperature dependent in the 40-60 °C range, suggesting enhanced internal protein motion as the system passes through the temperature range of the endotherm. These results suggest the pre-Tg endotherm is associated with a protein internal mobility transition.
authors.affiliation: School of Pharmacy, University of Connecticut, USA; Analytical Research Laboratories, Astellas Pharma Inc., Shizuoka, Japan. Electronic address: masayasu.mizuno@astellas.com.
authors.forename: Masayasu
authors.initials: M
authors.lastname: Mizuno
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Pikal
mesh_terms: D000818:Animals; D002417:Cattle; D002626:Chemistry, Pharmaceutical / Q000379:methods; D003903:Deuterium / Q000737:chemistry*; D005612:Freeze Drying / Q000379:methods; D005898:Glass / Q000737:chemistry; D006859:Hydrogen / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D044366:Transition Temperature
chemical_list: D012710:Serum Albumin, Bovine; D006859:Hydrogen; D003903:Deuterium
keywords: Amorphous; DSC; FTIR; Freeze-drying/lyophilization; Glass transition; Protein formulation; Protein internal dynamics; Solid state H/D exchange