title: High-throughput determination of Sudan Azo-dyes within powdered chili pepper by paper spray mass spectrometry.
abstract: A high-throughput mass spectrometric method is presented for the simultaneous detection of Sudan I, II, III, IV and Para-Red azo-dyes in foodstuff. The method is based on the use of paper spray mass spectrometry (MS) and deuterium-labeled internal standards on a triple-quadrupole instrument. A detailed assay of each azo-dye was performed by the isotope dilution method, through the precursor ion scan approach, using deuterium-labeled internal standards. The gas-phase breakdown pattern of each labeled and unlabeled analogue displays the naphthoic moiety as a common fragment. Sudan dyes can be determined above the threshold of 1 ppm. Paper spray allows for a wide range of analytes and samples to be investigated by MS in the open air and without sample preparation and bypassing chromatography.
authors.affiliation: Dipartimento di Chimica, Università della Calabria, Via P. Bucci Cubo 12/C, I-87036, Arcavacata di Rende (CS), Italy.
authors.forename: Domenico
authors.initials: D
authors.lastname: Taverna
authors.forename: Leonardo
authors.initials: L
authors.lastname: Di Donna
authors.forename: Fabio
authors.initials: F
authors.lastname: Mazzotti
authors.forename: Barbara
authors.initials: B
authors.lastname: Policicchio
authors.forename: Giovanni
authors.initials: G
authors.lastname: Sindona
mesh_terms: D001391:Azo Compounds / Q000032:analysis*; D002212:Capsicum / Q000737:chemistry*; D004396:Coloring Agents / Q000032:analysis*; D057166:High-Throughput Screening Assays / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*
chemical_list: D001391:Azo Compounds; D004396:Coloring Agents