title: Comparative serum proteomic analysis of adenomyosis using the isobaric tags for relative and absolute quantitation technique.
abstract: OBJECTIVE
  To identify differentially expressed proteins from the serum of women with and without adenomyosis and to explore the potential pathogenesis of adenomyosis.
  
  
  METHODS
  Serum samples from patients with adenomyosis were compared with samples from healthy controls.
  
  
  METHODS
  University hospital.
  
  
  METHODS
  Twenty patients with adenomyosis and 20 healthy volunteers.
  
  
  METHODS
  Collection of serum samples.
  
  
  METHODS
  Protein expression of serum was evaluated with iTRAQ (isobaric tags for relative and absolute quantitation) technology, and the validation of protein was performed with Western blot.
  
  
  RESULTS
  A total of 167 proteins were identified from 1,406 distinct peptides using iTRAQ technology. Twenty-five proteins were abnormally expressed in adenomyosis patients compared with the control group; 4 of these proteins were significantly down-regulated and 21 were significantly up-regulated in women with adenomyosis. Western blotting was used to validate the relative increases in serum protein levels for three of the identified proteins.
  
  
  CONCLUSIONS
  The differentially expressed proteins identified in our study are mainly involved in cell adhesion, the immune response, and the inflammatory response. On the basis of the results of this study, it can be concluded that these mechanisms may play an important role in the pathogenesis of adenomyosis. Additionally, these proteins may provide clues for not only a promising biomarker for the diagnosis of adenomyosis but also a potential target for therapeutic intervention.
authors.affiliation: Department of Obstetrics and Gynecology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing, People's Republic of China.
authors.forename: Long
authors.initials: L
authors.lastname: Xiaoyu
authors.forename: Zhang
authors.initials: Z
authors.lastname: Weiyuan
authors.forename: Jiang
authors.initials: J
authors.lastname: Ping
authors.forename: Wan
authors.initials: W
authors.lastname: Anxia
authors.forename: Zhou
authors.initials: Z
authors.lastname: Liane
mesh_terms: D062788:Adenomyosis / Q000097:blood* / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis / Q000097:blood; D001774:Blood Chemical Analysis; D015153:Blotting, Western; D016022:Case-Control Studies; D016000:Cluster Analysis; D005260:Female; D006801:Humans; D040901:Proteomics / Q000379:methods*; D054928:Validation Studies as Topic
chemical_list: D015415:Biomarkers
keywords: Adenomyosis; iTRAQ; proteomics; serum biomarkers