title: The RNAi machinery regulates growth and development in the filamentous fungus Trichoderma atroviride.
abstract: The RNAi machinery is generally involved in genome protection in filamentous fungi; however, the physiological role of RNAi has been poorly studied in fungal models. Here, we report that in the filamentous fungus Trichoderma atroviride, the products of the dcr2 and rdr3 genes control reproductive development, because mutations in these genes affect conidiation. In addition, Dcr1 together with Dcr2 control vegetative growth since Δdcr1, Δdcr2 and Δdcr1Δdcr2 present morphological alterations. Whole-genome transcriptional analysis of WT, Δdcr1, Δdcr2 and Δdcr1Δdcr2 show that each Dicer controls different biological processes, such as development or metabolism, which could explain the lack of conidiation in the mutants. Finally, we observed sRNAs that are differentially expressed in the WT and Δdcr2. The expression of some of these sRNAs correlates with the expression of differential transcripts, suggesting that these mRNAs may contain the corresponding targets. Together these data show that in T. atroviride, the RNAi machinery plays a central role in endogenous processes such as development and fitness, beyond controlling genome protection against invasive nucleic acids as reported for other fungi.
authors.affiliation: Laboratorio Nacional de Genómica para la Biodiversidad, Cinvestav Sede Irapuato, Km 9.6 Libramiento Norte Carretera Irapuato-León, 36821, Irapuato, Gto., Mexico.
authors.forename: Nohemi
authors.initials: N
authors.lastname: Carreras-Villaseñor
authors.forename: Edgardo U
authors.initials: EU
authors.lastname: Esquivel-Naranjo
authors.forename: J Manuel
authors.initials: JM
authors.lastname: Villalobos-Escobedo
authors.forename: Cei
authors.initials: C
authors.lastname: Abreu-Goodger
authors.forename: Alfredo
authors.initials: A
authors.lastname: Herrera-Estrella
mesh_terms: D005658:Fungi / Q000235:genetics* / Q000254:growth & development*; D017353:Gene Deletion; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D020868:Gene Silencing; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism*; D043244:Ribonuclease III / Q000235:genetics / Q000378:metabolism*; D013172:Spores, Fungal / Q000235:genetics / Q000254:growth & development; D014242:Trichoderma / Q000235:genetics* / Q000254:growth & development*
chemical_list: D034741:RNA, Small Interfering; D043244:Ribonuclease III