title: Bifidobacterium longum NCC3001 inhibits AH neuron excitability.
abstract: BACKGROUND
  Bifidobacterium longum (B. longum) NCC3001 can affect behavior and brain biochemistry, but identification of the cellular targets needs further investigation. Our hypothesis was that the communication with the brain might start with action on enteric sensory neurons.
  
  
  METHODS
  Ileal segments from adult mice were used to create a longitudinal muscle-myenteric-plexus preparation to expose sensory after-hyperpolarizing (AH) neurons in the myenteric plexus to allow access with microelectrodes. The intrinsic excitability of AH neurons was tested in response to the perfusion of conditioned media (B. longum culture supernatant) or unconditioned media (growth medium, MRS).
  
  
  RESULTS
  B. longum conditioned medium significantly reduced the excitability of AH neurons compared to perfusion with the unconditioned medium. Specifically, a reduction was seen in the number of action potentials fired per depolarizing test pulse, the instantaneous and time-dependent input resistances and the magnitude of the hyperpolarization-activated cationic current (Ih ).
  
  
  CONCLUSIONS
  The probiotic B. longum reduces excitability of AH sensory neurons likely via opening of potassium channels and closing of hyperpolarization-activated cation channels.
authors.affiliation: Department of Medicine, Faculty of Health Sciences, Farncombe Family Digestive Health Research Institute, McMaster University, Hamilton, Ontario, Canada.
authors.forename: A
authors.initials: A
authors.lastname: Khoshdel
authors.forename: E F
authors.initials: EF
authors.lastname: Verdu
authors.forename: W
authors.initials: W
authors.lastname: Kunze
authors.forename: P
authors.initials: P
authors.lastname: McLean
authors.forename: G
authors.initials: G
authors.lastname: Bergonzelli
authors.forename: J D
authors.initials: JD
authors.lastname: Huizinga
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D001644:Bifidobacterium / Q000378:metabolism*; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004594:Electrophysiology; D051379:Mice; D008839:Microelectrodes; D009197:Myenteric Plexus / Q000378:metabolism* / Q000382:microbiology*; D009474:Neurons / Q000378:metabolism* / Q000382:microbiology*
chemical_list: D017077:Culture Media, Conditioned