title: Saccharomonospora amisosensis sp. nov., isolated from deep marine sediment.
abstract: A novel actinomycete, strain DS3030(T), was isolated from a deep sediment sample, collected from the southern Black Sea coast, Turkey, and was examined using a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain DS3030(T) was shown to belong to the genus Saccharomonospora and to be related most closely to Saccharomonospora marina XMU15(T) (99.6 % similarity). Sequence similarities with other strains of the genus Saccharomonospora were lower than 97.0 %. The organism had chemical and morphological features typical of the genus Saccharomonospora. The cell wall of the novel strain contained meso-diaminopimelic acid, arabinose and galactose as diagnostic sugars. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The predominant menaquinone was MK-9(H4). Major fatty acids were iso-C16 : 0, iso-C16 : 0 2-OH and C16 : 1cis 9. Phenotypic data clearly distinguished the new isolate from its closest relative, S. marina XMU15(T). The combined genotypic and phenotypic data and low DNA-DNA relatedness with its closest related strain reveal that strain DS3030(T) represents a novel species of the genus, for which the name Saccharomonospora amisosensis sp. nov. is proposed. The type strain is DS3030(T) ( = DSM 45685(T) = KCTC 29069(T) = NRRL B-24885(T)).
authors.affiliation: Department of Biology, Faculty of Art and Science, Ondokuz Mayis University, 55139 Kurupelit-Samsun, Turkey.
authors.forename: Aysel
authors.initials: A
authors.lastname: Veyisoglu
authors.affiliation: Department of Biology, Faculty of Art and Science, Ondokuz Mayis University, 55139 Kurupelit-Samsun, Turkey.
authors.forename: Anil
authors.initials: A
authors.lastname: Sazak
authors.affiliation: Science Teaching Programme, Gazi Faculty of Education, Gazi University, Ankara, Turkey.
authors.forename: Demet
authors.initials: D
authors.lastname: Cetin
authors.affiliation: Anadolu University, Faculty of Science, Biology Department, 26470 Eskişehir, Turkey.
authors.forename: Kiymet
authors.initials: K
authors.lastname: Guven
authors.affiliation: Department of Biology, Faculty of Art and Science, Ondokuz Mayis University, 55139 Kurupelit-Samsun, Turkey.
authors.forename: Nevzat
authors.initials: N
authors.lastname: Sahin
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001089:Arabinose / Q000032:analysis; D001482:Base Composition; D059038:Black Sea; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005690:Galactose / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014421:Turkey; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9; D003960:Diaminopimelic Acid; D001089:Arabinose; D005690:Galactose