title: Effectiveness of egg yolk antibody against shiga toxin II variant toxicity in vitro and in vivo.
abstract: We prepared anti-Shiga toxin II variant (Stx2e) and anti-Stx2e B antibodies in chicken egg yolk and investigated their effectiveness in vitro and in vivo. Both Egg yolk antibodies (IgY) reduced the cytotoxic effects of Stx2e to Vero cells. The protective efficacy of anti-Stx2e IgY and anti-Stx2e B IgY was investigated in clean Kunming mice, which were experimentally induced with Stx2e. Peritoneal injection of anti-Stx2e IgY and anti-Stx2e B IgY apparently reduced the lethal effect of Stx2e to mice. These results indicate that both anti-Stx2e IgY and anti-Stx2e B IgY could protect mice from Stx2e challenge. Therefore, anti-Stx2e IgY or anti-Stx2e B IgY might be considered as potential candidate for therapeutics for porcine edema disease.
authors.affiliation: Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, Harbin 150030, Heilongjiang, China.
authors.forename: Yufei
authors.initials: Y
authors.lastname: Feng
authors.forename: Wenxin
authors.initials: W
authors.lastname: Liu
authors.forename: Dongfang
authors.initials: D
authors.lastname: Shi
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000907:Antibodies, Bacterial / Q000276:immunology*; D002642:Chick Embryo; D002645:Chickens; D002522:Chlorocebus aethiops; D004530:Egg Yolk / Q000737:chemistry* / Q000276:immunology; D005260:Female; D051379:Mice; D009500:Neutralization Tests; D022641:Shiga Toxin 2 / Q000276:immunology* / Q000633:toxicity*; D054323:Shiga-Toxigenic Escherichia coli / Q000276:immunology* / Q000502:physiology; D014709:Vero Cells
chemical_list: D000906:Antibodies; D000907:Antibodies, Bacterial; D022641:Shiga Toxin 2