title: H-rev107 regulates prostaglandin D2 synthase-mediated suppression of cellular invasion in testicular cancer cells.
abstract: BACKGROUND
  H-rev107 is a member of the HREV107 type II tumor suppressor gene family which includes H-REV107, RIG1, and HRASLS. H-REV107 has been shown to express at high levels in differentiated tissues of post-meiotic testicular germ cells. Prostaglandin D2 (PGD2) is conjectured to induce SRY-related high-mobility group box 9 (SOX9) expression and subsequent Sertoli cell differentiation. To date, the function of H-rev107 in differentiated testicular cells has not been well defined.
  
  
  RESULTS
  In the study, we found that H-rev107 was co-localized with prostaglandin D2 synthase (PTGDS) and enhanced the activity of PTGDS, resulting in increase of PGD2 production in testis cells. Furthermore, when H-rev107 was expressed in human NT2/D1 testicular cancer cells, cell migration and invasion were inhibited. Also, silencing of PTGDS would reduce H-rev107-mediated increase in PGD2, cAMP, and SOX9. Silencing of PTGDS or SOX9 also alleviated H-rev107-mediated suppression of cell migration and invasion.
  
  
  CONCLUSIONS
  These results revealed that H-rev107, through PTGDS, suppressed cell migration and invasion. Our data suggest that the PGD2-cAMP-SOX9 signal pathway might play an important role in H-rev107-mediated cancer cell invasion in testes.
authors.affiliation: Department of Internal Medicine, Buddhist Tzu Chi General Hospital Taipei Branch, New Taipei City, Taiwan.
authors.forename: Rong-Yaun
authors.initials: RY
authors.lastname: Shyu
authors.forename: Chang-Chieh
authors.initials: CC
authors.lastname: Wu
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Wang
authors.forename: Tzung-Chieh
authors.initials: TC
authors.lastname: Tsai
authors.forename: Lu-Kai
authors.initials: LK
authors.lastname: Wang
authors.forename: Mao-Liang
authors.initials: ML
authors.lastname: Chen
authors.forename: Shun-Yuan
authors.initials: SY
authors.lastname: Jiang
authors.forename: Fu-Ming
authors.initials: FM
authors.lastname: Tsai
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D019746:Intramolecular Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D054834:Lipocalins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009361:Neoplasm Invasiveness; D054512:Phospholipases A2, Calcium-Independent / Q000235:genetics* / Q000378:metabolism; D015230:Prostaglandin D2 / Q000235:genetics / Q000378:metabolism; D055758:SOX9 Transcription Factor / Q000235:genetics / Q000378:metabolism; D012708:Sertoli Cells / Q000378:metabolism / Q000473:pathology; D015398:Signal Transduction; D013736:Testicular Neoplasms / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D054834:Lipocalins; D055758:SOX9 Transcription Factor; D054512:Phospholipases A2, Calcium-Independent; C490066:Plaat3 protein, mouse; D019746:Intramolecular Oxidoreductases; C022466:prostaglandin R2 D-isomerase; D015230:Prostaglandin D2