title: Survey of Enterobacteriaceae contamination of table eggs collected from layer flocks in Australia.
abstract: In the present study, eggs from commercial caged layer flocks at different stages of lay in Australia were collected. Enterobacteriaceae populations from eggshell surface and eggshell pore were enumerated and these populations characterized using API® Rapid 20E strips. The eggshell surface, eggshell pore and egg internal content samples were also processed for the isolation of Salmonella and these isolates were tested for the presence or absence of several virulence genes (prgH, sopB, spiC, orfL, invA, sifA, sitC, misL). Results indicated that there was no significant difference in total Enterobacteriaceae count on the eggs of the flock from early, mid or late lay flocks. Enterobacteriaceae isolates were of 11 different genera which included: Cedecea, Citrobacter, Enterobacter, Escherichia, Klebsiella, Kluyvera, Leclercia, Pantoea, Salmonella, Serratia and Yersinia. Out of all 153 identified Enterobacteriaceae isolates, the Escherichia genus was reported most frequently (60.78%). Results also indicated that overall there were 4.51% (14/310) Salmonella positive pooled samples. In this study, 14 Salmonella strains were isolated, serotyping confirmed that 12 out of them were Salmonella Infantis and the 2 others were Salmonella enterica subsp. enterica serovar 4,12:d: Polymerase chain reaction results indicated that all Salmonella Infantis isolates harboured invA, misL, orfL, prgH, sifA, sitC, sopB and spiC genes which suggests that Salmonella Infantis strains isolated from eggshell surface may have the capacity to invade and survive in macrophages.
authors.affiliation: School of Animal and Veterinary Sciences, The University of Adelaide, Roseworthy, 5173, Australia.
authors.forename: V C
authors.initials: VC
authors.lastname: Gole
authors.forename: K K
authors.initials: KK
authors.lastname: Chousalkar
authors.forename: J R
authors.initials: JR
authors.lastname: Roberts
mesh_terms: D000818:Animals; D001315:Australia; D058491:Bacterial Load; D002645:Chickens; D002954:Citrobacter / Q000302:isolation & purification; D004528:Egg Shell / Q000382:microbiology*; D004531:Eggs / Q000382:microbiology*; D004754:Enterobacter / Q000302:isolation & purification; D004755:Enterobacteriaceae / Q000145:classification / Q000302:isolation & purification*; D005516:Food Microbiology; D005798:Genes, Bacterial; D012475:Salmonella / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D019779:Salmonella enterica / Q000235:genetics / Q000302:isolation & purification