title: In vivo optical coherence tomography (OCT) in periocular basal cell carcinoma: correlations between in vivo OCT images and postoperative histology.
abstract: OBJECTIVE
  To investigate in vivo optical coherence tomography (OCT) for imaging of periocular basal cell carcinoma (BCC).
  
  
  METHODS
  Consecutive patients with periocular BCC were prospectively investigated with VivoSight OCT imaging prior to surgical excision. Histology sections were compared with OCT images with regard to lesion measurements (x, y and z dimensions) and histological features.
  
  
  RESULTS
  A total of 15 patients with biopsy proven BCC were recruited. The OCT horizontal margins correlated positively with histology (r=0.8 and 0.66, x and y axes) and could be identified in 3/15 (x axis) and 6/15 (y axis) cases. The vertical margin correlation was r=0.43 and BCC depth could be measured in 9/15 cases. The following histological features of BCC could be identified on OCT images: (1) lobular pattern (100%); (2) dilated blood vessels (80%); (3) reflective margins of tumour lobules (100%); and (4) epidermal thinning overlying BCC lobules (100%).
  
  
  CONCLUSIONS
  This study indicated a strong positive correlation between the margins of periocular BCCs measured using in vivo OCT and histology, and a weak positive correlation with depth of invasion. VivoSight OCT produced high resolution images of BCC morphology. The limitations in horizontal margin measurements could potentially be overcome by design modification of the scanning probe.
authors.affiliation: Ophthalmology Department, Maidstone & Tunbridge Wells NHS Trust, Maidstone, Kent, UK.
authors.forename: Lucia
authors.initials: L
authors.lastname: Pelosini
authors.forename: Henry Barnabas
authors.initials: HB
authors.lastname: Smith
authors.forename: John B
authors.initials: JB
authors.lastname: Schofield
authors.forename: Adam
authors.initials: A
authors.lastname: Meeckings
authors.forename: Anish
authors.initials: A
authors.lastname: Dhital
authors.forename: Mona
authors.initials: M
authors.lastname: Khandwala
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D002280:Basal Cell Carcinoma / Q000175:diagnosis*; D005142:Eyelid Neoplasms / Q000175:diagnosis*; D005143:Eyelids / Q000473:pathology; D005260:Female; D005583:Fourier Analysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects; D011446:Prospective Studies; D012878:Skin Neoplasms / Q000175:diagnosis*; D013223:Statistics as Topic; D041623:Tomography, Optical Coherence
keywords: Eye Lids; Imaging; Neoplasia; Pathology; Treatment Surgery