title: Zirex: a novel zinc-regulated expression system for Lactococcus lactis.
abstract: Here, we report a new zinc-inducible expression system for Lactococcus lactis, called Zirex, consisting of the pneumococcal repressor SczA and PczcD. PczcD tightly regulates the expression of green fluorescent protein in L. lactis. We show the applicability of Zirex together with the nisin-controlled expression system, enabling simultaneous but independent regulation of different genes.
authors.affiliation: Department of Molecular Genetics, University of Groningen, Linnaeusborg, Groningen, The Netherlands.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Mu
authors.forename: Manuel
authors.initials: M
authors.lastname: Montalbán-López
authors.forename: Yoshimitsu
authors.initials: Y
authors.lastname: Masuda
authors.forename: Oscar P
authors.initials: OP
authors.lastname: Kuipers
mesh_terms: D015964:Gene Expression Regulation, Bacterial; D049452:Green Fluorescent Proteins / Q000378:metabolism; D013294:Lactococcus lactis / Q000235:genetics* / Q000378:metabolism; D009561:Nisin / Q000235:genetics / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D015032:Zinc / Q000378:metabolism*
chemical_list: D012097:Repressor Proteins; D009561:Nisin; D049452:Green Fluorescent Proteins; C102526:nisin A; D015032:Zinc