title: Topographical and chemical effects of electrochemically assisted deposited hydroxyapatite coatings on osteoblast-like cells.
abstract: A recently commercialised hydroxyapatite electrochemically assisted chemical deposition technique (BoneMaster) has been shown to induce increased bone apposition; whether this response is caused by the surface topography or chemistry is unknown. An in-vitro examination using human osteoblast-like cells was performed on a series of BoneMaster-coated surfaces. The chemistry was separated from the topography using a thin gold coating; Thermanox coverslips were used as a control. BoneMaster surfaces showed significantly greater alkaline phosphatase activity and osteocalcin production compared with controls; however, no difference was found between the gold-coated and uncoated BoneMaster samples, indicating topography is the main contributing factor.
authors.affiliation: 1University of Oxford, Botnar Research Centre, Nuffield Orthopaedic Centre, Oxford, UK.
authors.forename: Elise C
authors.initials: EC
authors.lastname: Pegg
authors.forename: Faisal
authors.initials: F
authors.lastname: Matboli
authors.forename: Tim
authors.initials: T
authors.lastname: Marriott
authors.forename: Imran
authors.initials: I
authors.lastname: Khan
authors.forename: Colin A
authors.initials: CA
authors.lastname: Scotchford
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D045744:Cell Line, Tumor; D020099:Coated Materials, Biocompatible; D017886:Durapatite; D055664:Electrochemical Techniques / Q000379:methods*; D006801:Humans; D010006:Osteoblasts / Q000166:cytology* / Q000201:enzymology; D056951:Photoelectron Spectroscopy; D013499:Surface Properties
chemical_list: D020099:Coated Materials, Biocompatible; D017886:Durapatite; D000469:Alkaline Phosphatase
keywords: Fixation; bone; chemistry; hydroxyapatite; surface; topography