title: A convenient synthesis of GDP-D-rhamnose: the donor substrate for D-rhamnosyltransferase WbpZ from Pseudomonas aeruginosa PAO1.
abstract: Gram negative bacteria have lipopolysaccharides (LPS) that are critical for their survival. LPS molecules are composed of antigenic exopolysaccharide chains (O antigens). We are interested in discovering the enzymes involved in the biosynthesis of O antigens in Pseudomonas aeruginosa. The common polysaccharide antigen contains α-linked D-rhamnose residues. We have now synthesized GDP-D-rhamnose by a convenient synthesis in aqueous solution, and have shown that it can be used without extensive purification as the donor substrate for D-rhamnosyltransferase (WbpZ) from the P. aeruginosa strain PAO1. The availability of this nucleotide sugar preparation allows for characterization of D-rhamnosyltransferases.
authors.affiliation: Department of Medicine, Queen's University, Kingston, Ontario, Canada K7L 3N6.
authors.forename: Shuo
authors.initials: S
authors.lastname: Wang
authors.forename: Hidenori
authors.initials: H
authors.lastname: Tanaka
authors.forename: Ole
authors.initials: O
authors.lastname: Hindsgaul
authors.forename: Joseph S
authors.initials: JS
authors.lastname: Lam
authors.forename: Inka
authors.initials: I
authors.lastname: Brockhausen
mesh_terms: D006156:Guanosine Diphosphate Sugars / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D006602:Hexosyltransferases / Q000378:metabolism*; D011550:Pseudomonas aeruginosa / Q000201:enzymology* / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: C422949:GDP-rhamnose; D006156:Guanosine Diphosphate Sugars; D006602:Hexosyltransferases; C025816:LPS rhamnosyltransferases