title: TALEN-mediated editing of the mouse Y chromosome.
abstract: The functional study of Y chromosome genes has been hindered by a lack of mouse models with specific Y chromosome mutations. We used transcription activator-like effector nuclease (TALEN)-mediated gene editing in mouse embryonic stem cells (mESCs) to produce mice with targeted gene disruptions and insertions in two Y-linked genes--Sry and Uty. TALEN-mediated gene editing is a useful tool for dissecting the biology of the Y chromosome.
authors.affiliation: Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA.
authors.forename: Haoyi
authors.initials: H
authors.lastname: Wang
authors.forename: Yueh-Chiang
authors.initials: YC
authors.lastname: Hu
authors.forename: Styliani
authors.initials: S
authors.lastname: Markoulaki
authors.forename: G Grant
authors.initials: GG
authors.lastname: Welstead
authors.forename: Albert W
authors.initials: AW
authors.lastname: Cheng
authors.forename: Chikdu S
authors.initials: CS
authors.lastname: Shivalila
authors.forename: Tatyana
authors.initials: T
authors.lastname: Pyntikova
authors.forename: Daniel B
authors.initials: DB
authors.lastname: Dadon
authors.forename: Daniel F
authors.initials: DF
authors.lastname: Voytas
authors.forename: Adam J
authors.initials: AJ
authors.lastname: Bogdanove
authors.forename: David C
authors.initials: DC
authors.lastname: Page
authors.forename: Rudolf
authors.initials: R
authors.lastname: Jaenisch
mesh_terms: D000818:Animals; D053595:Embryonic Stem Cells / Q000378:metabolism; D004720:Endonucleases / Q000235:genetics* / Q000378:metabolism; D018390:Gene Targeting; D050173:Genes, Y-Linked; D051379:Mice; D015778:Minor Histocompatibility Antigens; D009154:Mutation; D011506:Proteins / Q000235:genetics*; D055749:SOXB2 Transcription Factors / Q000235:genetics* / Q000378:metabolism; D014998:Y Chromosome / Q000235:genetics*
chemical_list: D015778:Minor Histocompatibility Antigens; D011506:Proteins; D055749:SOXB2 Transcription Factors; C102450:Uty protein, mouse; D004720:Endonucleases