title: Nucleoside analog labeling of neural stem cells and their progeny.
abstract: Nucleoside analog pulse labeling is an important technique which can assess the birthdate, cell cycle maintenance, or cycling rates of cells during development. This method has evolved over several decades of use and is now applied to a multitude of tissue subtypes and systems. The methodology in this chapter covers the classic uses for analog pulse labeling as well as their use in conjunction with the newly characterized technique of in utero electroporation (IUE).
authors.affiliation: Department of Neuroscience and Cell Biology, UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ, USA.
authors.forename: Erik Michael
authors.initials: EM
authors.lastname: DeBoer
authors.forename: Mladen-Roko
authors.initials: MR
authors.lastname: Rasin
mesh_terms: D000818:Animals; D001947:Breeding; D002453:Cell Cycle; D019070:Cell Lineage; D018274:Electroporation; D005260:Female; D007150:Immunohistochemistry; D051379:Mice; D058953:Neural Stem Cells / Q000166:cytology*; D009705:Nucleosides / Q000378:metabolism*; D011247:Pregnancy; D012996:Solutions; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D009705:Nucleosides; D012996:Solutions