title: Decontamination of materials contaminated with Bacillus anthracis and Bacillus thuringiensis Al Hakam spores using PES-Solid, a solid source of peracetic acid.
abstract: OBJECTIVE
  To develop test methods and evaluate survival of Bacillus anthracis Ames, B. anthracis ∆Sterne and B. thuringiensis Al Hakam spores after exposure to PES-Solid (a solid source of peracetic acid), including PES-Solid formulations with bacteriostatic surfactants.
  
  
  RESULTS
  Spores (≥ 7 logs) were dried on seven different test materials and treated with three different PES-Solid formulations (or preneutralized controls) at room temperature for 15 min. There was either no spore survival or less than 1 log (<10 spores) of spore survival in 56 of 63 test combinations (strain, formulation and substrate). Less than 2.7 logs (<180 spores) survived in the remaining seven test combinations. The highest spore survival rates were seen on water-dispersible chemical agent resistant coating (CARC-W) and Naval ship topcoat (NTC). Electron microscopy and Coulter analysis showed that all spore structures were intact after spore inactivation with PES-Solid.
  
  
  CONCLUSIONS
  Three PES-Solid formulations inactivated Bacillus spores that were dried on seven different materials.
  
  
  CONCLUSIONS
  A test method was developed to show that PES-Solid formulations effectively inactivate Bacillus spores on different materials.
authors.affiliation: CBR Concepts and Experimentation Branch (Z21), Naval Surface Warfare Center, Dahlgren Division, Dahlgren, VA 22448-5162, USA. DLGR_NSWC_Z20@navy.mil
authors.forename: T L
authors.initials: TL
authors.lastname: Buhr
authors.forename: C M
authors.initials: CM
authors.lastname: Wells
authors.forename: A A
authors.initials: AA
authors.lastname: Young
authors.forename: Z A
authors.initials: ZA
authors.lastname: Minter
authors.forename: C A
authors.initials: CA
authors.lastname: Johnson
authors.forename: A N
authors.initials: AN
authors.lastname: Payne
authors.forename: D C
authors.initials: DC
authors.lastname: McPherson
mesh_terms: D001408:Bacillus anthracis / Q000187:drug effects* / Q000648:ultrastructure; D001413:Bacillus thuringiensis / Q000187:drug effects* / Q000648:ultrastructure; D003666:Decontamination / Q000379:methods*; D004202:Disinfectants / Q000737:chemistry / Q000494:pharmacology*; D010463:Peracetic Acid / Q000494:pharmacology*; D013171:Spores, Bacterial / Q000187:drug effects / Q000648:ultrastructure
chemical_list: D004202:Disinfectants; D010463:Peracetic Acid
keywords: Bacillus; PES-Solid; decontamination; peracetic acid; spore