title: The culture of limbal epithelial cells.
abstract: The transplantation of cultured limbal epithelial cells (LEC) has since its first application in 1997 emerged as a promising technique for treating limbal stem cell deficiency. The culture methods hitherto used vary with respect to preparation of the harvested tissue, choice of culture medium, culture time, culture substrates, and supplementary techniques. In this chapter, we describe a procedure for establishing human LEC cultures using a feeder-free explant culture technique with human amniotic membrane (AM) as the culture substrate.
authors.affiliation: Department of Medical Biochemistry, Oslo University Hospital, Oslo, Norway.
authors.forename: Tor Paaske
authors.initials: TP
authors.lastname: Utheim
authors.forename: Torstein
authors.initials: T
authors.lastname: Lyberg
authors.forename: Sten
authors.initials: S
authors.lastname: Ræder
mesh_terms: D000650:Amnion; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods; D003470:Culture Media; D004847:Epithelial Cells; D019573:Epithelium, Corneal / Q000166:cytology*; D006801:Humans; D016850:Limbus Corneae / Q000166:cytology*; D046509:Tissue Culture Techniques
chemical_list: D003470:Culture Media