title: Comperative study of catalase immobilization on chitosan, magnetic chitosan and chitosan-clay composite beads.
abstract: Catalase was immobilized on chitosan and modified chitosan. Studies were carried out on free-immobilized catalase concerning the determination of optimum temperature, pH, thermal, storage stability, reusability, and kinetic parameters. Optimum temperature and pH for free catalase and catalase immobilized were found as 35°C and 7.0, respectively. After 100 times of repeated tests, the immobilized catalases on chitosan-clay and magnetic chitosan maintain over 50% and 60% of the original activity, respectively. The ease of catalase immobilization on low-cost matrices and good stability upon immobilization in the present study make it a suitable product for further use in the food industry.
authors.affiliation: Faculty of Science and Arts, Department of Chemistry, Celal Bayar University , Muradiye, Manisa , Turkey.
authors.forename: Esra
authors.initials: E
authors.lastname: Başak
authors.forename: Tülin
authors.initials: T
authors.lastname: Aydemir
authors.forename: Ayşe
authors.initials: A
authors.lastname: Dinçer
authors.forename: Seda Çınar
authors.initials: SÇ
authors.lastname: Becerik
mesh_terms: D000538:Aluminum Silicates / Q000737:chemistry*; D000818:Animals; D002374:Catalase / Q000737:chemistry* / Q000378:metabolism; D002417:Cattle; D048271:Chitosan / Q000737:chemistry*; D000077215:Clay; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008863:Microspheres; D012117:Resins, Synthetic / Q000737:chemistry; D013696:Temperature
chemical_list: D000538:Aluminum Silicates; D004800:Enzymes, Immobilized; D058185:Magnetite Nanoparticles; D012117:Resins, Synthetic; D048271:Chitosan; D002374:Catalase; D000077215:Clay