title: Antimüllerian hormone inhibits follicle-stimulating hormone-induced adenylyl cyclase activation, aromatase expression, and estradiol production in human granulosa-lutein cells.
abstract: OBJECTIVE
  To investigate the effects of antimüllerian hormone (AMH) on basal and FSH-induced cytochrome P450 aromatase (aromatase) expression and E2 production in human granulosa-lutein (hGL) cells, and to elucidate the mechanism by which AMH exerts its effects.
  
  
  METHODS
  Experimental study.
  
  
  METHODS
  Academic medical center for reproductive science.
  
  
  METHODS
  The hGL cells were obtained from consenting patients undergoing IVF treatment.
  
  
  METHODS
  None.
  
  
  METHODS
  Primary cultures of hGL cells were used to examine the effects of AMH (10 ng/mL) on basal and FSH (0.2 IU/mL)-stimulated E2 and intracellular cyclic adenosine 3':5' monophosphate (cAMP) accumulation, as well as aromatase and FSH receptor expression. Small interfering RNA targeting type II AMH receptor (AMHR2) was used to verify the specificity of the effects.
  
  
  RESULTS
  Treatment with AMH significantly reduced FSH-stimulated aromatase expression and E2 accumulation, whereas it had no measurable effects on basal and/or 8-Br-cAMP-stimulated levels. The FSH receptor messenger RNA and protein levels were not altered in AMH-treated cells. Cotreatment with AMH suppressed FSH-induced increases in intracellular cAMP. Knockdown of AMHR2 reversed the effects of AMH on aromatase expression.
  
  
  CONCLUSIONS
  The AMH acts through AMHR2 to inhibit FSH-induced adenylyl cyclase activation, aromatase expression, and E2 production.
authors.affiliation: Department of Obstetrics and Gynaecology, Child and Family Research Institute, University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: Hsun-Ming
authors.initials: HM
authors.lastname: Chang
authors.forename: Christian
authors.initials: C
authors.lastname: Klausen
authors.forename: Peter C K
authors.initials: PC
authors.lastname: Leung
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism*; D054304:Anti-Mullerian Hormone / Q000494:pharmacology*; D001141:Aromatase / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D015536:Down-Regulation / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D004958:Estradiol / Q000378:metabolism*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D006801:Humans; D008184:Luteal Cells / Q000187:drug effects* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000494:pharmacology; D011962:Receptors, FSH / Q000235:genetics / Q000378:metabolism; D018000:Receptors, Peptide / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D018125:Receptors, Transforming Growth Factor beta / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism
chemical_list: D034741:RNA, Small Interfering; D011962:Receptors, FSH; D018000:Receptors, Peptide; D018125:Receptors, Transforming Growth Factor beta; C084621:anti-Mullerian hormone receptor; D004958:Estradiol; D054304:Anti-Mullerian Hormone; D005640:Follicle Stimulating Hormone; D001141:Aromatase; D000262:Adenylyl Cyclases
keywords: AMH; AMHR2; E(2); adenylyl cyclase; aromatase