title: Functionalized layered double hydroxide nanoparticles conjugated with disulfide-linked polycation brushes for advanced gene delivery.
abstract: Layered double hydroxides (LDHs) have aroused great attention as potential nanosized drug delivery carriers, but independent inorganic LDH wrapped with DNA shows very low transfection efficiency. To manipulate and control the surface properties of LDH nanoparticles is of crucial importance in the designing of LDH-based drug carriers. In this work, surface-initiated atom transfer radical polymerization (ATRP) of 2-(dimethylamino)ethyl methacrylate (DMAEMA) is employed to tailor the functionality of LDH surfaces in a well-controlled manner and produce a series of well-defined novel gene delivery vectors (termed as LDH-PDs), where a flexible three-step method was first developed to introduce the ATRP initiation sites containing disulfide bonds onto LDH surfaces. In comparison the pristine LDH particles, the resultant LDH-PDs exhibited better ability to condense plasmid DNA (pDNA) and much higher levels to delivery genes in different cell lines including COS7 and HepG2 cell lines. Moreover, the LDH-PDs also could largely enhance cellular uptake. This present study demonstrates that functionalization of bioinorganic LDH with flexible polycation brushes is an effective means to produce new LDH-based gene delivery systems.
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, College of Materials Science and Engineering, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: H
authors.initials: H
authors.lastname: Hu
authors.forename: K M
authors.initials: KM
authors.lastname: Xiu
authors.forename: S L
authors.initials: SL
authors.lastname: Xu
authors.forename: W T
authors.initials: WT
authors.lastname: Yang
authors.forename: F J
authors.initials: FJ
authors.lastname: Xu
mesh_terms: D000818:Animals; D019556:COS Cells; D002412:Cations / Q000737:chemistry / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D004247:DNA / Q000737:chemistry; D004220:Disulfides / Q000737:chemistry* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000737:chemistry* / Q000494:pharmacology; D056945:Hep G2 Cells; D006801:Humans; D006878:Hydroxides / Q000737:chemistry* / Q000494:pharmacology; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D010957:Plasmids; D011108:Polymers / Q000737:chemistry* / Q000494:pharmacology; D013329:Structure-Activity Relationship; D013499:Surface Properties; D013696:Temperature
chemical_list: D002412:Cations; D004220:Disulfides; D006878:Hydroxides; D011108:Polymers; D004247:DNA; C031356:hydroxide ion