title: Induction of B7-H6, a ligand for the natural killer cell-activating receptor NKp30, in inflammatory conditions.
abstract: B7-H6, a member of the B7 family of immunoreceptors, is as a cell-surface ligand for the NKp30-activating receptor expressed on natural killer cells. B7-H6 is not detected in normal human tissues at steady state but is expressed on tumor cells. However, whether B7-H6 can be expressed in other conditions remains unknown. We analyzed here the pathways that lead to the expression of B7-H6 in nontransformed cells. In vitro, B7-H6 was induced at the surface of CD14(+)CD16(+) proinflammatory monocytes and neutrophils upon stimulation by ligands of Toll-like receptors or proinflammatory cytokines such as interleukin-1β and tumor necrosis factor α. In these conditions, a soluble form of B7-H6 (sB7-H6) was also produced by activated monocytes and neutrophils. In vivo, B7-H6 was expressed on circulating proinflammatory CD14(+)CD16(+) monocytes in a group of patients in sepsis conditions, and was linked to an increased mortality. sB7-H6 was selectively detected in the sera of patients with gram-negative sepsis and was associated with membrane vesicles that co-sedimented with the exosomal fraction. These findings reveal that B7-H6 is not only implicated in tumor immunosurveillance but also participates in the inflammatory response in infectious conditions.
authors.affiliation: Centre d'Immunologie de Marseille-Luminy, Institut National de la Santé et de la Recherche Médicale, Marseille Cedex 09, France.
authors.forename: Jessica
authors.initials: J
authors.lastname: Matta
authors.forename: Myriam
authors.initials: M
authors.lastname: Baratin
authors.forename: Laurent
authors.initials: L
authors.lastname: Chiche
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Forel
authors.forename: Céline
authors.initials: C
authors.lastname: Cognet
authors.forename: Guillemette
authors.initials: G
authors.lastname: Thomas
authors.forename: Catherine
authors.initials: C
authors.lastname: Farnarier
authors.forename: Christelle
authors.initials: C
authors.lastname: Piperoglou
authors.forename: Laurent
authors.initials: L
authors.lastname: Papazian
authors.forename: Damien
authors.initials: D
authors.lastname: Chaussabel
authors.forename: Sophie
authors.initials: S
authors.lastname: Ugolini
authors.forename: Frédéric
authors.initials: F
authors.lastname: Vély
authors.forename: Eric
authors.initials: E
authors.lastname: Vivier
mesh_terms: D000911:Antibodies, Monoclonal / Q000378:metabolism; D060887:B7 Antigens / Q000097:blood / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism; D002478:Cells, Cultured; D006801:Humans; D007249:Inflammation / Q000276:immunology* / Q000473:pathology; D008024:Ligands; D009000:Monocytes / Q000378:metabolism; D055628:Natural Cytotoxicity Triggering Receptor 3 / Q000378:metabolism*; D009504:Neutrophils / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018805:Sepsis / Q000276:immunology / Q000473:pathology; D012995:Solubility
chemical_list: D000911:Antibodies, Monoclonal; D060887:B7 Antigens; D008024:Ligands; C542363:NCR3LG1 protein, human; D055628:Natural Cytotoxicity Triggering Receptor 3; D012333:RNA, Messenger