title: Quantitative analysis of betaine in Lycii Fructus by HILIC-ELSD.
abstract: A rapid and simple high-performance liquid chromatography (HPLC) method with evaporative light scattering detection (ELSD) was developed for the determination of betaine from Lycii Fructus. Betaine was separated with an Atlantis hydrophilic interaction liquid chromatography silica column (4.6 × 150 mm, 5 μm, 100 Å) by isocratic elution using 30 mM ammonium acetate buffer and acetonitrile (20:80, v/v %) as the mobile phase. The flow rate was 1.0 mL/min, and the temperature for the spray chamber and drift tube was set at 30 and 50 °C, respectively. The method was fully validated with respect to linearity, precision, accuracy, stability and robustness. The HPLC/ELSD method was applied successfully to the quantification of betaine in the extract of Lycii Fructus. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of twenty-six L. barbarum L. from China (BC01-BC26), 3 L. barbarum L. (BJ27-BJ29) from Japan, 12 L. chinense Miller from China (CC30-CC41) and 51 L. chinense Miller samples (CK42-CK92) from Korea. The results indicate that the established HPLC/ELSD method is suitable for quality evaluation of Lycii Fructus.
authors.affiliation: College of Pharmacy, Catholic University of Daegu, Gyeongsan, 712-702, South Korea.
authors.forename: Bing Tian
authors.initials: BT
authors.lastname: Zhao
authors.forename: Su Yang
authors.initials: SY
authors.lastname: Jeong
authors.forename: Kyoung
authors.initials: K
authors.lastname: Hwangbo
authors.forename: Dong Cheul
authors.initials: DC
authors.lastname: Moon
authors.forename: Eun-Kyoung
authors.initials: EK
authors.lastname: Seo
authors.forename: Dongho
authors.initials: D
authors.lastname: Lee
authors.forename: Je-Hyun
authors.initials: JH
authors.lastname: Lee
authors.forename: Byung-Sun
authors.initials: BS
authors.lastname: Min
authors.forename: Eun-Sook
authors.initials: ES
authors.lastname: Ma
authors.forename: Jong Keun
authors.initials: JK
authors.lastname: Son
authors.forename: Mi Hee
authors.initials: MH
authors.lastname: Woo
mesh_terms: D001622:Betaine / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005638:Fruit / Q000737:chemistry; D008027:Light; D032305:Lycium / Q000737:chemistry*; D012542:Scattering, Radiation
chemical_list: D001622:Betaine