title: [Improving effect of Shengjingsan on spermatogenic function following testicular torsion/detorsion in rats and its mechanism].
abstract: OBJECTIVE
  To study the effect of Shengjingsan on spermatogenic function following testicular torsion/detorsion in rats and its action mechanism.
  
  
  METHODS
  Forty SD male rats were equally randomized to groups A (sham operation), B (control), C (low-dose Shengjingsan), D (medium-dose Shengjingsan) and E (high-dose Shengjingsan). The model of testicular torsion was established by 720 degrees clockwise torsion of the left testis for 4 hours. An hour before operation, the rats of group B received daily gavage of normal saline at 1 ml per kg per d, while those in groups C, D and E that of Shengjingsan at 0.01, 0.02 and 0.03 g per kg per d, all for 35 days. Then all the rats were sacrificed for measuring the semen parameters by CASA and detecting the expression of the CatSper1 gene in the sperm by RT-PCR.
  
  
  RESULTS
  Compared with group A, Sperm concentration, the percentage of grade a + b sperm, sperm vitality and CatSper1 expression were significantly lower in group B ([15.30 +/- 6.30] %, [44.42 +/- 6.36] %, [21.00 +/- 6.14] x 10(6)/ml and 1.12 +/- 0.50) than in A ([51.30 +/- 6.60]%, [69.01 +/- 7.20]%, [40.53 +/- 7.01] x 10(6)/ml and 2.04 +/- 0.77) (P < 0.01). Compared with group B, the four parameters were increased remarkably in groups D ([51.63 +/- 3.20] %, [72.09 +/- 2.20]%, [55.30 +/- 5.90] x10(6)/ml and 2.11 +/- 0.20) andE ([55.93 +/- 3.17]%, [73.01 +/- 2.11]%, [58.33 + 4.90] x 10(6)/ml and 2.31 +/- 0.17) (P < 0.01), but not significantly in C ([18.02 +/- 0.23]%, [48.04 +/- 7.01]%, [22.87 +/- 2.10] x 10(6)/ml and 1.19 +/- 0.51) (P > 0.05).
  
  
  CONCLUSIONS
  Shengjingsan can improve sperm parameters following testicular torsion/ detorsion in male rats by regulating their spermatogenic function and improving the expression of CatSper1 in the sperm.
authors.affiliation: Department of Urology, Laiwu Hospital Affiliated to Taishan Medical College, Laiwu, Shandong 271100, China. lwwsp719@163.com
authors.forename: Shi-Ping
authors.initials: SP
authors.lastname: Wang
authors.forename: Yan-Hui
authors.initials: YH
authors.lastname: Zhai
authors.forename: Ke-Liang
authors.initials: KL
authors.lastname: Xie
authors.forename: Li-Jiang
authors.initials: LJ
authors.lastname: Sun
authors.forename: Yan-Jiang
authors.initials: YJ
authors.lastname: Li
authors.forename: Xin-Sheng
authors.initials: XS
authors.lastname: Wang
mesh_terms: D000818:Animals; D015220:Calcium Channels / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013076:Sperm Count; D013086:Spermatic Cord Torsion / Q000378:metabolism* / Q000503:physiopathology; D013091:Spermatogenesis / Q000187:drug effects*; D013094:Spermatozoa / Q000378:metabolism*
chemical_list: D015220:Calcium Channels; C579284:CatSper1 protein, rat; D004365:Drugs, Chinese Herbal