title: Nanostructured CaCO₃-poly(ethyleneimine) microparticles for phenol sensing in fluidic microsystem.
abstract: A new and simple strategy based on nanostructured CaCO₃-poly(ethyleneimine) (PEI) microparticles (MPs) for phenol sensing using PDMS/glass fluidic microchip is developed. This fluidic microsystem including integrated screen-printed electrodes modified with CaCO₃-PEI MPs and tyrosinase (Tyr) through cross-linking with glutaraldehyde, represents a low-cost platform for phenol detection. The designed fluidic microsystem improves the sensitivity of the biosensor allowing the detection of very low concentrations of phenol (up to 10 nM). This device shows high repeatability and low detection limit, is easy to be fabricated, inexpensive, disposable, and amenable to mass production.
authors.affiliation: ICN2-Institut Catala de Nanociencia i Nanotecnologia, Campus UAB, Bellaterra, Barcelona, Spain.
authors.forename: Carmen C
authors.initials: CC
authors.lastname: Mayorga-Martinez
authors.forename: Lenka
authors.initials: L
authors.lastname: Hlavata
authors.forename: Sandrine
authors.initials: S
authors.lastname: Miserere
authors.forename: Adaris
authors.initials: A
authors.lastname: López-Marzo
authors.forename: Jan
authors.initials: J
authors.lastname: Labuda
authors.forename: Josefina
authors.initials: J
authors.lastname: Pons
authors.forename: Arben
authors.initials: A
authors.lastname: Merkoçi
mesh_terms: D000363:Agaricales / Q000201:enzymology; D015374:Biosensing Techniques / Q000295:instrumentation; D002119:Calcium Carbonate / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000378:metabolism; D004867:Equipment Design; D057230:Limit of Detection; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D014442:Monophenol Monooxygenase / Q000378:metabolism; D019800:Phenol / Q000032:analysis*; D011094:Polyethyleneimine / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D019800:Phenol; D011094:Polyethyleneimine; D014442:Monophenol Monooxygenase; D002119:Calcium Carbonate
keywords: Electrochemistry; Fluidic microsystem; Nanostructurated CaCO3-poly(ethylene-imine) microparticles; Phenolic compounds