title: Comparison of Cytotoxic Activity of Anticancer Drugs against Various Human Tumor Cell Lines Using In Vitro Cell-Based Approach.
abstract: Chemotherapy is the main treatment modality for certain types of cancer. It is important to monitor and ensure that these chemotherapeutic drugs are potent and effective prior to patient administration. The objective of the study is to evaluate the cytotoxic activity and potency of selected commercially available generic anticancer drugs in comparison with originator using various human cancer cell lines in an in vitro cell-based assay. Half-maximal inhibitory concentration (IC50) of the different chemotherapeutic agents was obtained from an experimentally derived dose-response curve. Relative potency of the drugs was estimated according to Parallel Line assay. This study demonstrated that the selected generic oncology products tested had similar efficacy compared with the originator. Both products showed comparable results as shown both in vitro cytotoxicity assay and statistical analysis. In vitro cell-based cytotoxicity assay promises to be a useful, reliable and rapid method for demonstrating chemotherapeutic drug activity.
authors.affiliation: Department of Biological Sciences, Medical and Regulatory Affairs Division, United Laboratories, Inc., 66 United St. Mandaluyong City, Philippines.
authors.forename: Leila
authors.initials: L
authors.lastname: Florento
authors.forename: Ronald
authors.initials: R
authors.lastname: Matias
authors.forename: Elena
authors.initials: E
authors.lastname: Tuaño
authors.forename: Katherine
authors.initials: K
authors.lastname: Santiago
authors.forename: Frederick
authors.initials: F
authors.lastname: Dela Cruz
authors.forename: Alexander
authors.initials: A
authors.lastname: Tuazon
keywords: IC50; dose-response; in vitro cytotoxicity; relative potency