title: Claudin-8 expression in Sertoli cells and putative spermatogonial stem cells in the bovine testis.
abstract: Adhesion molecules are expressed by both adult and embryonic stem cells, with different classes of adhesion molecules involved in cell-membrane and intercellular contacts. In this study the expression of the adhesion molecule claudin-8 (CLDN8), a tight-junction protein, was investigated as a potential marker for undifferentiated spermatogonia in the bovine testis. We found that CLDN8 was expressed by both spermatogonia and a subset of Sertoli cells in the bovine testis. We also showed co-expression of GFRα1 in testis cells with CLDN8 and with Dolichos biflorus agglutinin-fluorescein isothiocyanate (DBA-FITC) staining. We observed co-enrichment of spermatogonia and CLDN8-expressing Sertoli cells in DBA-FITC-assisted magnetic-activated cell sorting (MACS), an observation supported by results from fluorescence-activated cell sorting analysis, which showed CLDN8-expressing cells were over-represented in the MACS-positive cell fraction, leading to the hypothesis that CLDN8 may play a role in the spermatogonial stem-cell niche.
authors.affiliation: CSIRO Food Futures National Research Flagship, North Ryde, NSW 2113, Australia.
authors.forename: Mary
authors.initials: M
authors.lastname: McMillan
authors.affiliation: CSIRO Animal, Food and Health Sciences, F. D. McMaster Laboratory, Armidale, NSW 2350, Australia.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Andronicos
authors.affiliation: CSIRO Food Futures National Research Flagship, North Ryde, NSW 2113, Australia.
authors.forename: Rhonda
authors.initials: R
authors.lastname: Davey
authors.affiliation: CSIRO Food Futures National Research Flagship, North Ryde, NSW 2113, Australia.
authors.forename: Sally
authors.initials: S
authors.lastname: Stockwell
authors.affiliation: School of Environmental and Rural Science, University of New England, Armidale, NSW 2351, Australia.
authors.forename: Geoff
authors.initials: G
authors.lastname: Hinch
authors.affiliation: CSIRO Food Futures National Research Flagship, North Ryde, NSW 2113, Australia.
authors.forename: Sabine
authors.initials: S
authors.lastname: Schmoelzl
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology / Q000378:metabolism*; D000818:Animals; D002417:Cattle; D002469:Cell Separation; D057167:Claudins / Q000378:metabolism*; D005434:Flow Cytometry; D051097:Glial Cell Line-Derived Neurotrophic Factor Receptors / Q000378:metabolism; D008297:Male; D012708:Sertoli Cells / Q000166:cytology / Q000378:metabolism*; D055153:Stem Cell Niche / Q000502:physiology; D013737:Testis / Q000166:cytology* / Q000378:metabolism
chemical_list: D057167:Claudins; D051097:Glial Cell Line-Derived Neurotrophic Factor Receptors; C502011:claudin 8