title: Influence of amino acids Shiff bases on irradiated DNA stability in vivo.
abstract: To reveal protective role of the new Mn(II) complexes with Nicotinyl-L-Tyrosinate and Nicotinyl-L-Tryptophanate Schiff Bases against ionizing radiation. The DNA of the rats liver was isolated on 7, 14, and 30 days after X-ray irradiation. The differences between the DNA of irradiated rats and rats pre-treated with Mn(II) complexes were studied using the melting, microcalorimetry, and electrophoresis methods. The melting parameters and the melting enthalpy of rats livers DNA were changed after the X-ray irradiation: melting temperature and melting enthalpy were decreased and melting interval was increased. These results can be explained by destruction of DNA molecules. It was shown that pre-treatment of rats with Mn(II) complexes approximates the melting parameters to norm. Agarose gel electrophoresis data confirmed the results of melting studies. The separate DNA fragments were revealed in DNA samples isolated from irradiated animals. The DNA isolated from animals pre-treated with the Mn(II) chelates had better electrophoretic characteristics, which correspond to healthy DNA. Pre-treatment of the irradiated rats with Mn(II)(Nicotinil-L-Tyrosinate) and Mn(II)(Nicotinil-L-Tryptophanate)2 improves the DNA characteristics.
authors.affiliation: Department of Physics, Yerevan State University, Al. Manoogian 1, 0025, Yerevan, Armenia, nelkarapetyan@gmail.com.
authors.forename: N H
authors.initials: NH
authors.lastname: Karapetyan
authors.forename: M H
authors.initials: MH
authors.lastname: Malakyan
authors.forename: S A
authors.initials: SA
authors.lastname: Bajinyan
authors.forename: A L
authors.initials: AL
authors.lastname: Torosyan
authors.forename: I E
authors.initials: IE
authors.lastname: Grigoryan
authors.forename: S G
authors.initials: SG
authors.lastname: Haroutiunian
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D000818:Animals; D002151:Calorimetry; D056831:Coordination Complexes / Q000737:chemistry / Q000494:pharmacology; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D004249:DNA Damage / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D008297:Male; D008345:Manganese / Q000737:chemistry; D009539:Nicotinic Acids / Q000737:chemistry; D011839:Radiation, Ionizing; D051381:Rats; D017208:Rats, Wistar; D012545:Schiff Bases / Q000737:chemistry / Q000494:pharmacology*; D014364:Tryptophan / Q000031:analogs & derivatives / Q000737:chemistry; D014443:Tyrosine / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D000596:Amino Acids; D056831:Coordination Complexes; D009539:Nicotinic Acids; D012545:Schiff Bases; C555004:nicotinyl tryptophanate; C555003:nicotinyl tyrosinate; D014443:Tyrosine; D008345:Manganese; D014364:Tryptophan; D004247:DNA