title: Basolateral BMP signaling in polarized epithelial cells.
abstract: Bone morphogenetic proteins (BMPs) regulate various biological processes, mostly mediated by cells of mesenchymal origin. However, the roles of BMPs in epithelial cells are poorly understood. Here, we demonstrate that, in polarized epithelial cells, BMP signals are transmitted from BMP receptor complexes exclusively localized at the basolateral surface of the cell membrane. In addition, basolateral stimulation with BMP increased expression of components of tight junctions and enhanced the transepithelial resistance (TER), counteracting reduction of TER by treatment with TGF-β or an anti-tumor drug. We conclude that BMPs maintain epithelial polarity via intracellular signaling from basolaterally localized BMP receptors.
authors.affiliation: Department of Biochemistry, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Chuo, Yamanashi, Japan. msaitoh-ind@umin.ac.jp
authors.forename: Masao
authors.initials: M
authors.lastname: Saitoh
authors.forename: Takuya
authors.initials: T
authors.lastname: Shirakihara
authors.forename: Akira
authors.initials: A
authors.lastname: Fukasawa
authors.forename: Kana
authors.initials: K
authors.lastname: Horiguchi
authors.forename: Kei
authors.initials: K
authors.lastname: Sakamoto
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sugiya
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Beppu
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Fujita
authors.forename: Ikuo
authors.initials: I
authors.lastname: Morita
authors.forename: Kohei
authors.initials: K
authors.lastname: Miyazono
authors.forename: Keiji
authors.initials: K
authors.lastname: Miyazawa
mesh_terms: D000818:Animals; D055415:Bone Morphogenetic Protein 4 / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D055418:Bone Morphogenetic Protein 6 / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D052004:Bone Morphogenetic Protein Receptors / Q000235:genetics* / Q000378:metabolism; D002460:Cell Line; D016764:Cell Polarity / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology; D004285:Dogs; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D015870:Gene Expression; D055427:Growth Differentiation Factor 2 / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D051379:Mice; D032446:Myoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D015398:Signal Transduction; D019108:Tight Junctions / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000494:pharmacology
chemical_list: C525810:Bmp4 protein, mouse; C525817:Bmp6 protein, mouse; D055415:Bone Morphogenetic Protein 4; D055418:Bone Morphogenetic Protein 6; C526108:Gdf2 protein, mouse; D055427:Growth Differentiation Factor 2; D016212:Transforming Growth Factor beta; D052004:Bone Morphogenetic Protein Receptors; D002945:Cisplatin