title: Antiobesity effects of quercetin-rich onion peel extract on the differentiation of 3T3-L1 preadipocytes and the adipogenesis in high fat-fed rats.
abstract: The aim of the present study was to examine the effect of quercetin-rich onion peel extract (OPE) on anti-differentiation in 3T3-L1 preadipocytes and the antiobesity in high-fat fed rats. We found that lipid accumulations and TG contents in 3T3-L1 cells were markedly suppressed by OPE. The mRNA levels of activating protein (AP2) were down-regulated and those of carnitine palmitoyl transferase-1 α (CPT-1α) and fatty acid binding protein 4 (FABP4) were up-regulated by 75 and 100 μg/ml OPE. Body weight, retroperitoneal and mesenteric fat weights of SD rats were significantly lower in the 8 week high fat (HF) diet+0.72% OPE group than in the HF group. Peroxisome proliferator-activated receptor (PPAR)γ mRNA levels were down-regulated in the epididymal fat of OPE than those of control and HF, and significant down-regulation of CCAAT/enhancer binding protein (C/EBP)α mRNA levels in OPE was also observed than the control. The mRNA levels of CPT-1α and uncoupling protein-1 (UCP-1) were up-regulated by the OPE, while those of fatty acid synthase (FAS) and acetyl-CoA carboxylase (ACC) were down-regulated in HF and OPE groups compared to control group. These results suggest that quercentin-enriched OPE may have antiobesity effects by suppressing preadipocyte differentiation and inhibiting adipogenesis.
authors.affiliation: Department of Food and Nutrition, Korea University, Seoul 136-703, Republic of Korea.
authors.forename: Jiyoung
authors.initials: J
authors.lastname: Moon
authors.forename: Hyun-Ju
authors.initials: HJ
authors.lastname: Do
authors.forename: Oh Yoen
authors.initials: OY
authors.lastname: Kim
authors.forename: Min-Jeong
authors.initials: MJ
authors.lastname: Shin
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects*; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D019440:Anti-Obesity Agents / Q000494:pharmacology*; D001483:Base Sequence; D002454:Cell Differentiation / Q000187:drug effects*; D017931:DNA Primers; D059305:Diet, High-Fat; D050356:Lipid Metabolism / Q000235:genetics; D008297:Male; D051379:Mice; D019697:Onions / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D011794:Quercetin / Q000032:analysis*; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D015430:Weight Gain / Q000187:drug effects
chemical_list: D019440:Anti-Obesity Agents; D017931:DNA Primers; D010936:Plant Extracts; D012333:RNA, Messenger; D011794:Quercetin
keywords: 3T3-L1; ACC; AP2; Adipogenesis; Antiobesity; CPT-1α; FABP4; FAS; High-fat diet; OPE; Onion peel extract; Quercetin; acetyl-CoA carboxylase; activating protein; carnitine palmitoyltransferase-1 α; fatty acid binding protein 4; fatty acid synthase; onion peel extract