title: Metal-organic frameworks-based biosensor for sequence-specific recognition of double-stranded DNA.
abstract: A simple, cost-efficient, sensitive and selective fluorescence sensor is developed for sequence-specific recognition of duplex DNA (ds-DNA) in vitro using metal-organic framework (MOF) as the sensing platform. N,N-Bis(2-hydroxy-ethyl)dithiooxamidatocopper(II) (H(2)dtoaCu) was chosen as the example MOF, because it strongly chemisorbs the dye-labeled probe TFO (triplex-forming oligonucleotide), and quenches fluorescence from the dye. In the presence of target ds-DNA (the PPT of HIV RNA, a 16-bp ds-DNA sequence), the TFO could interact with the major groove in ds-DNA (via Hoogsteen hydrogen bonding) to form a rigid triplex structure, resulting in fluorescence recovery. The enhanced fluorescence signal has a relationship with the ds-DNA concentration, the detection limit is as low as 1.3 nmol L(-1) (S/N = 3) with good selectivity, which is lower than that based on a graphene oxide platform and electrochemical-DNA sensor.
authors.affiliation: State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China.
authors.forename: Lifen
authors.initials: L
authors.lastname: Chen
authors.forename: Hanye
authors.initials: H
authors.lastname: Zheng
authors.forename: Xi
authors.initials: X
authors.lastname: Zhu
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Lin
authors.forename: Longhua
authors.initials: L
authors.lastname: Guo
authors.forename: Bin
authors.initials: B
authors.lastname: Qiu
authors.forename: Guonan
authors.initials: G
authors.lastname: Chen
authors.forename: Zhong-Ning
authors.initials: ZN
authors.lastname: Chen
mesh_terms: D001483:Base Sequence; D015374:Biosensing Techniques / Q000379:methods*; D003300:Copper / Q000737:chemistry; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000235:genetics*; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D009942:Organometallic Compounds / Q000737:chemistry*; D013379:Substrate Specificity
chemical_list: D009942:Organometallic Compounds; C051802:triplex DNA; D003300:Copper; D004247:DNA