title: Interaction between IGF1 and IGFBPs in bovine cystic ovarian disease.
abstract: Cystic ovarian disease (COD) is one of the main factors responsible for reproductive disorders in cattle. Although the pathogenesis and mechanism of cyst formation are not fully understood, it has been proposed that the IGF system could play an essential role, as it is a key intraovarian regulator. The aim of the present study was to determine whether the altered levels in IGF1 detected in bovines with COD are associated with changes at mRNA level or with differential modulation by IGFBPs. The mRNA levels of the IGF components studied were analyzed by real time PCR and in situ hybridization, and IGFBP expression and activity were assayed by immunohistochemistry and ligand blot, respectively. Results showed a decreased IGF1 mRNA level due to a lower granulosa cell gene expression in cystic follicles (P<0.05). Results also showed variations in IGFBP expression in the intraovarian cellular compartment and concentration in follicular fluid, and suggest that IGFBP3 is a key regulator of intrafollicular IGF1 in animals with COD.
authors.affiliation: Laboratorio de Biología Celular y Molecular Aplicada, Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Argentina; Instituto de Ciencias Veterinarias del Litoral ICiVet-Litoral, Consejo Nacional de Investigaciones Científicas y Tecnológicas CONICET, Argentina.
authors.forename: F M
authors.initials: FM
authors.lastname: Rodríguez
authors.forename: N R
authors.initials: NR
authors.lastname: Salvetti
authors.forename: M
authors.initials: M
authors.lastname: Colombero
authors.forename: M L
authors.initials: ML
authors.lastname: Stangaferro
authors.forename: C G
authors.initials: CG
authors.lastname: Barbeito
authors.forename: H H
authors.initials: HH
authors.lastname: Ortega
authors.forename: F
authors.initials: F
authors.lastname: Rey
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D002417:Cattle / Q000378:metabolism*; D005260:Female; D015571:Follicular Fluid / Q000378:metabolism; D007150:Immunohistochemistry / Q000662:veterinary; D017403:In Situ Hybridization / Q000662:veterinary; D018969:Insulin-Like Growth Factor Binding Proteins / Q000235:genetics / Q000378:metabolism*; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism*; D010048:Ovarian Cysts / Q000378:metabolism / Q000662:veterinary*; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary
chemical_list: D018969:Insulin-Like Growth Factor Binding Proteins; D012333:RNA, Messenger; D007334:Insulin-Like Growth Factor I