title: [Investigation of bacterial diversity in the biological desulfurization reactor for treating high salinity wastewater by the 16S rDNA cloning method].
abstract: The bacterial diversity in the biological desulfurization reactor operated continuously for 1 year was studied by the 16S rDNA cloning and sequencing method. Forty clones were randomly selected and their partial 16S rDNA genes (ca. 1,400 bp) were sequenced and blasted. The results indicated that there were dominant bacterias in the biological desulfurization reactor, where 33 clones belonged to 3 different published phyla, while 1 clone belonged to unknown phylum. The dominant bacterial community in the system was Proteobacteria, which accounted for 85.3%. The bacterial community succession was as follows: the gamma-Proteobacteria(55.9%), beta-Proteobacteria(17.6%), Actinobacteridae (8.8%), delta-Proteobacteria (5.9%) , alpha-Proteobacteria(5.9%), and Sphingobacteria (2.9%). Halothiobacillus sp. ST15 and Thiobacillus sp. UAM-I were the major desulfurization strains.
authors.affiliation: College of Resources and Environmental Engineering, Wuhan University of Science and Technology, Wuhan 430070, China. liuweiguo@bipt.edu.cn
authors.forename: Wei-Guo
authors.initials: WG
authors.lastname: Liu
authors.forename: Cun-Zhen
authors.initials: CZ
authors.lastname: Liang
authors.forename: Jin-Sheng
authors.initials: JS
authors.lastname: Yang
authors.forename: Gui-Ping
authors.initials: GP
authors.lastname: Wang
authors.forename: Miao-Miao
authors.initials: MM
authors.lastname: Liu
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D044822:Biodiversity; D019149:Bioreactors / Q000382:microbiology*; D003198:Computer Simulation; D004269:DNA, Bacterial / Q000235:genetics; D015723:Gene Library; D020560:Proteobacteria / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D013455:Sulfur / Q000302:isolation & purification* / Q000378:metabolism; D014865:Waste Disposal, Fluid / Q000379:methods; D062065:Wastewater / Q000737:chemistry*
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D013455:Sulfur; D062065:Wastewater