title: Leukocyte cell population data (volume conductivity scatter) in postprandial leukocyte activation.
abstract: BACKGROUND
  Changes in leukocyte cell population data have been reported in various infectious diseases, but little is known in other inflammatory conditions such as the postprandial state. We investigated whether leukocyte cell population data change during postprandial leukocyte activation.
  
  
  METHODS
  Healthy volunteers underwent a standardized oral fat loading test (OFLT). Flowcytometric quantitation of leukocyte activation markers CD11b, CD66b, CD35, and CD36, together with leukocyte cell population data from LH750 hematology analyzers were measured fasting and at 4 and 8 h postprandially.
  
  
  RESULTS
  Twelve volunteers were included. Postprandial leukocyte activation was confirmed by increased expression of CD11b by monocytes (+11.7%) and neutrophils (+15.0%) and by increased expression of CD66b (+14.7%) and CD35 (+16.6%) by neutrophils at T = 4 h. The mean scatter from neutrophils, reflecting granularity, significantly decreased at T = 4 h (P < 0.05) and returned to baseline at T = 8 h (P-anova 0.048). The mean volume of monocytes increased significantly at T = 4 h (P < 0.001) and returned to baseline at T = 8 h (P-anova 0.0008). At T = 4 h, CD11b expression on neutrophils was associated with a reduction in mean scatter of neutrophils (Pearson's r: -0.677, P = 0.016).
  
  
  CONCLUSIONS
  Postprandial leukocyte activation is accompanied by temporary changes in leukocyte cell population data, similar to changes observed during various infections, but to a lesser extent.
authors.affiliation: Department of Internal Medicine, Sint Franciscus Gasthuis, Rotterdam, The Netherlands.
authors.forename: B
authors.initials: B
authors.lastname: Klop
authors.forename: G-J M
authors.initials: GJ
authors.lastname: van de Geijn
authors.forename: T L
authors.initials: TL
authors.lastname: Njo
authors.forename: H W
authors.initials: HW
authors.lastname: Janssen
authors.forename: A P
authors.initials: AP
authors.lastname: Rietveld
authors.forename: A
authors.initials: A
authors.lastname: van Miltenburg
authors.forename: L
authors.initials: L
authors.lastname: Fernández-Sender
authors.forename: J W F
authors.initials: JW
authors.lastname: Elte
authors.forename: M
authors.initials: M
authors.lastname: Castro Cabezas
mesh_terms: D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D007958:Leukocyte Count; D007962:Leukocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism; D009504:Neutrophils / Q000378:metabolism; D019518:Postprandial Period; D013997:Time Factors; D014280:Triglycerides / Q000097:blood; D055815:Young Adult
chemical_list: D015415:Biomarkers; D014280:Triglycerides
keywords: LH750 analyzer; Postprandial lipemia; leukocyte activation; leukocyte cell population data; volume conductivity scatter