title: Effective embryoid body formation from induced pluripotent stem cells for regeneration of respiratory epithelium.
abstract: OBJECTIVE
  We have previously demonstrated the potential use of induced pluripotent stem (iPS) cells for regeneration of respiratory epithelium by culturing embryoid bodies (EBs). The aim of the present study was to determine the most effective conditions for EB formation from iPS cells for regeneration of respiratory epithelium.
  
  
  METHODS
  Experimental study.
  
  
  METHODS
  iPS cells cultured on a gelatin-coated dish were seeded on low-attachment plates for generating EBs. Under several conditions including the air-liquid interface (ALI) method, with varying cell numbers and suspension times, EBs were transferred to a gelatin-coated dish supplemented with growth factors. The shape, size, aggregation, and adhesion of EBs for iPS cell differentiation were evaluated, and the cultured tissue was histologically examined.
  
  
  RESULTS
  EBs appropriate for differentiation were observed using 1,000 cells after 5 days of suspension culture. Respiratory epithelium-like tissue was histologically observed. The ciliary epithelium was confirmed immunohistologically.
  
  
  CONCLUSIONS
  Based on the varying suspension times and cell numbers with the ALI method, this study presented effective conditions for EB formation from iPS cells for regeneration of respiratory epithelium.
authors.affiliation: Department of Otolaryngology, Fukushima Medical University, Fukushima, Japan.
authors.forename: Koshi
authors.initials: K
authors.lastname: Otsuki
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Imaizumi
authors.forename: Yukio
authors.initials: Y
authors.lastname: Nomoto
authors.forename: Mika
authors.initials: M
authors.lastname: Nomoto
authors.forename: Ikuo
authors.initials: I
authors.lastname: Wada
authors.forename: Masao
authors.initials: M
authors.lastname: Miyake
authors.forename: Koichi
authors.initials: K
authors.lastname: Omori
mesh_terms: D002478:Cells, Cultured; D058732:Embryoid Bodies; D057026:Induced Pluripotent Stem Cells; D012038:Regeneration; D020545:Respiratory Mucosa / Q000502:physiology*
keywords: Induced pluripotent stem cells; ciliated cells; embryoid bodies; respiratory epithelium