title: Evaluation of CD4+CD25+ T lymphocyte response time kinetics in patients with chronic Chagas disease after in vitro stimulation with recombinant Trypanosoma cruzi antigens.
abstract: BACKGROUND
  CD4+CD25+ T lymphocytes have been implicated in the regulation of host inflammatory response against Trypanosoma cruzi, and may be involved in the clinical course of the disease.
  
  
  METHODS
  Peripheral blood mononuclear cells from patients with chronic Chagas disease were cultured in the presence of T. cruzi recombinant antigens and assayed for lymphocytes at distinct time points.
  
  
  RESULTS
  It was possible to differentiate clinical forms of chronic Chagas disease at days 3 and 5 according to presence of CD4+CD25+ T cells in cell cultures.
  
  
  CONCLUSIONS
  Longer periods of cell culture proved to be potentially valuable for prospective evaluations of CD4+CD25+ T lymphocytes in patients with chronic Chagas disease.
authors.affiliation: Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE, Brazil.
authors.forename: Suellen Carvalho de Moura
authors.initials: SC
authors.lastname: Braz
authors.forename: Virginia Maria Barros de
authors.initials: VM
authors.lastname: Lorena
authors.forename: Adriene Siqueira
authors.initials: AS
authors.lastname: Melo
authors.forename: Maria da Glória Aureliano Melo
authors.initials: Mda G
authors.lastname: Cavalcanti
authors.forename: Yara de Miranda
authors.initials: Yde M
authors.lastname: Gomes
mesh_terms: D000953:Antigens, Protozoan / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D014355:Chagas Disease / Q000276:immunology*; D002908:Chronic Disease; D006801:Humans; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology*; D007700:Kinetics; D007963:Leukocytes, Mononuclear / Q000469:parasitology; D013997:Time Factors; D014349:Trypanosoma cruzi / Q000276:immunology*
chemical_list: D000953:Antigens, Protozoan; D053645:Interleukin-2 Receptor alpha Subunit