title: Antifibrotic activity of galangin, a novel function evaluated in animal liver fibrosis model.
abstract: This study aimed to investigate the effects of galangin on liver fibrosis in rats induced by subcutaneous injection of carbon tetrachloride (CCl4). The administration of CCl4 to rats for 12 weeks caused significant increase of hyaluronic acid, laminin, alanine transaminase, aspartate transaminase and decrease of total protein, albumin in serum, while the influences could be reversed by galangin. Galangin markedly reduced hepatic malondialdehyde, hydroxyproline concentration, increased activities of liver superoxide dismutase, glutathione peroxidase compared with CCl4-treated rats. Histological results indicated that galangin alleviated liver damage. In addition, treatment with galangin significantly down-regulated expressions of α-smooth muscle actin and transforming growth factor β1. These results suggest galangin can inhibit liver fibrosis induced by CCl4 in rats, which was probably associated with its effect on removing oxygen free radicals, decreasing lipid peroxidation, as well as inhibiting hepatic stellate cells activation and proliferation.
authors.affiliation: Department of Pharmacology, China Pharmaceutical University, Nanjing, China.
authors.forename: Xinhui
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Pharmacology, China Pharmaceutical University, Nanjing, China. Electronic address: gonggq@hotmail.com.
authors.forename: Guoqing
authors.initials: G
authors.lastname: Gong
authors.affiliation: Department of Pharmacology, China Pharmaceutical University, Nanjing, China.
authors.forename: Wenhui
authors.initials: W
authors.lastname: Yang
authors.affiliation: Department of Pharmacology, China Pharmaceutical University, Nanjing, China.
authors.forename: Yunzhan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Pharmacology, China Pharmaceutical University, Nanjing, China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Jiang
authors.affiliation: Department of Pharmacology, China Pharmaceutical University, Nanjing, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Li
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002251:Carbon Tetrachloride; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000494:pharmacology*; D055166:Hepatic Stellate Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008106:Liver Cirrhosis, Experimental / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D015415:Biomarkers; D005419:Flavonoids; C037032:galangin; D002251:Carbon Tetrachloride
keywords: ALB; ALT; AST; CCl(4); CMC-Na; ECM; GSH-Px; HA; HI; HSCs; Hyp; LN; MDA; ROS; SOD; TGF-β1; TP; alanine transaminase; albumin; aspartate transaminase; carbon tetrachloride; carboxymethyl cellulose-Na; extracellular matrix; glutathione peroxidase; hepatic index; hepatic stellate cells; hyaluronic acid; hydroxyproline; laminin; malondialdehyde; reactive oxygen species; superoxide dismutase; total protein; transforming growth factor β1; α-SMA; α-smooth muscle actin