title: Quantifying continuous-flow dielectrophoretic trapping of cells and micro-particles on micro-electrode array.
abstract: An interdigitated electrode array embedded within a micro-channel with forced flow is shown to enable dielectrophoretic (DEP) characterization of particles and/or cells based on measurements of their trapping percentage over a continuous frequency range. A simplified model of the trapping percentage, using spatial averaging of the convective and DEP force, linearly correlated it to the effective DEP force (in its positive mode). Thus, the Clausius-Mossotti factor was fitted to the experimental data, yielding effective electrical characteristics of the particles and/or cells. Also, the generated trapping percentage curve response over a continuous range of frequencies facilitates sorting and detection based on differences other than just the cross-over frequencies.
authors.affiliation: Russel Berrie Nanotechnology Institute RBNI, Technion - Israel Institute of Technology, Haifa, Israel.
authors.forename: Lichen
authors.initials: L
authors.lastname: Rozitsky
authors.forename: Amir
authors.initials: A
authors.lastname: Fine
authors.forename: Dekel
authors.initials: D
authors.lastname: Dado
authors.forename: Shahar
authors.initials: S
authors.lastname: Nussbaum-Ben-Shaul
authors.forename: Shulamit
authors.initials: S
authors.lastname: Levenberg
authors.forename: Gilad
authors.initials: G
authors.lastname: Yossifon
mesh_terms: D000818:Animals; D002460:Cell Line; D002469:Cell Separation; D055252:Cell-Derived Microparticles / Q000737:chemistry*; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism; D004867:Equipment Design; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D006801:Humans; D051379:Mice; D046228:Microarray Analysis / Q000379:methods*; D008839:Microelectrodes; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D008863:Microspheres; D008962:Models, Theoretical; D011137:Polystyrenes / Q000737:chemistry
chemical_list: D011137:Polystyrenes