title: Skin and hair on-a-chip: in vitro skin models versus ex vivo tissue maintenance with dynamic perfusion.
abstract: Substantial progress has been achieved over the last few decades in the development of skin equivalents to model the skin as an organ. However, their static culture still limits the emulation of essential physiological properties crucial for toxicity testing and compound screening. Here, we describe a dynamically perfused chip-based bioreactor platform capable of applying variable mechanical shear stress and extending culture periods. This leads to improvements of culture conditions for integrated in vitro skin models, ex vivo skin organ cultures and biopsies of single hair follicular units.
authors.affiliation: Technische Universität Berlin, Institute of Biotechnology, Berlin, Germany.
authors.forename: Beren
authors.initials: B
authors.lastname: Ataç
authors.forename: Ilka
authors.initials: I
authors.lastname: Wagner
authors.forename: Reyk
authors.initials: R
authors.lastname: Horland
authors.forename: Roland
authors.initials: R
authors.lastname: Lauster
authors.forename: Uwe
authors.initials: U
authors.lastname: Marx
authors.forename: Alexander G
authors.initials: AG
authors.lastname: Tonevitsky
authors.forename: Reza P
authors.initials: RP
authors.lastname: Azar
authors.forename: Gerd
authors.initials: G
authors.lastname: Lindner
mesh_terms: D019149:Bioreactors; D002450:Cell Communication; D024062:Collagen Type V / Q000378:metabolism; D018859:Hair Follicle / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D007633:Keratins / Q000378:metabolism; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D008954:Models, Biological; D009924:Organ Culture Techniques / Q000295:instrumentation*; D012867:Skin / Q000166:cytology* / Q000378:metabolism; D013314:Stress, Mechanical
chemical_list: D024062:Collagen Type V; D007633:Keratins