title: The study of the proteome of healthy human blood plasma under conditions of long-term confinement in an isolation chamber.
abstract: We identified changes in the proteome of healthy human blood plasma caused by exposure to 105-day confinement in an isolation chamber. After removal of major proteins and concentration of minor proteins, plasma fractions were analyzed by two-dimensional electrophoresis followed by identification of significantly different protein spots by mass spectrometric analysis of the peptide fragments. The levels of α- and β-chains of fibrinogen, a fragment of complement factor C4, apolipoproteins AI and E, plasminogen factor C1 complement, and immunoglobulin M changed in participants during the isolation period. These changes probably reflect the adaptive response to altered conditions of life.
authors.affiliation: Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia. oxana.trifonova@gmail.com
authors.forename: O P
authors.initials: OP
authors.lastname: Trifonova
authors.forename: L Kh
authors.initials: LKh
authors.lastname: Pastushkova
authors.forename: N F
authors.initials: NF
authors.lastname: Samenkova
authors.forename: A L
authors.initials: AL
authors.lastname: Chernobrovkin
authors.forename: I I
authors.initials: II
authors.lastname: Karuzina
authors.forename: A V
authors.initials: AV
authors.lastname: Lisitsa
authors.forename: I M
authors.initials: IM
authors.lastname: Larina
mesh_terms: D000222:Adaptation, Physiological; D000328:Adult; D016632:Apolipoprotein A-I / Q000097:blood; D001057:Apolipoproteins E / Q000097:blood; D001798:Blood Proteins / Q000032:analysis*; D003172:Complement C1 / Q000378:metabolism; D003181:Complement C4 / Q000378:metabolism; D020378:Confined Spaces; D015180:Electrophoresis, Gel, Two-Dimensional; D005340:Fibrinogen / Q000378:metabolism; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D008297:Male; D013058:Mass Spectrometry; D010949:Plasma / Q000737:chemistry*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics
chemical_list: D016632:Apolipoprotein A-I; D001057:Apolipoproteins E; D001798:Blood Proteins; D003172:Complement C1; D003181:Complement C4; D007075:Immunoglobulin M; D020543:Proteome; D005340:Fibrinogen