title: Fluorescent molecularly imprinted polymer thin films for specific protein detection prepared with dansyl ethylenediamine-conjugated O-acryloyl L-hydroxyproline.
abstract: Protein-imprinted polymers, capable of specific transduction of protein binding events into fluorescent signal change, were designed and synthesized by using dansyl ethylenediamine-conjugated O-acryloyl L-hydroxyproline (Hyp-En-Dans). Human serum albumin (HSA) was used as a model target protein and HSA-imprinted polymers (HSA-IP) were prepared on glass substrates. Specific fluorescence change was observed for HSA binding on the imprinted polymer thin film, whereas a weaker response was observed for other proteins, including bovine serum albumin, chymotrypsin, lysozyme, and avidin. The binding specificity was found to derive from the rigid structure of the hydrogen-bondable pyrrolidine moiety. Compared with SPR measurements, the non-specific binding caused by the polymer matrix and/or randomly located fluorescent monomer residues that did not compose specific binding sites did not contribute to the observed fluorescence change. These results revealed that the proposed protein-imprinting technique using Hyp-En-Dans could provide a highly selective protein-sensing platform, in which only specific binding events would be detected by fluorescent measurements.
authors.affiliation: Graduate School of Engineering, Kobe University, 1-1 Rokkoudai-cho, Nada-ku, Kobe 657-8501, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Inoue
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kuwahara
authors.forename: Kohei
authors.initials: K
authors.lastname: Ohmori
authors.forename: Hirobumi
authors.initials: H
authors.lastname: Sunayama
authors.forename: Tooru
authors.initials: T
authors.lastname: Ooya
authors.forename: Toshifumi
authors.initials: T
authors.lastname: Takeuchi
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D002417:Cattle; D003619:Dansyl Compounds / Q000737:chemistry*; D005029:Ethylenediamines / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D006909:Hydroxyproline / Q000737:chemistry*; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry; D012680:Sensitivity and Specificity; D012710:Serum Albumin, Bovine / Q000032:analysis* / Q000302:isolation & purification; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D003619:Dansyl Compounds; D005029:Ethylenediamines; D005456:Fluorescent Dyes; D011108:Polymers; D012710:Serum Albumin, Bovine; C032377:dansylethylenediamine; D006909:Hydroxyproline