title: Isolation, characterization and complete genome sequence of PhaxI: a phage of Escherichia coli O157 : H7.
abstract: Bacteriophages are considered as promising biological agents for the control of infectious diseases. Sequencing of their genomes can ascertain the absence of antibiotic resistance, toxin or virulence genes. The anti-O157 : H7 coliphage, PhaxI, was isolated from a sewage sample in Iran. Morphological studies by transmission electron microscopy showed that it has an icosahedral capsid of 85-86 nm and a contractile tail of 115×15 nm. PhaxI contains dsDNA composed of 156 628 nt with a G+C content of 44.5 mol% that encodes 209 putative proteins. In MS analysis of phage particles, 92 structural proteins were identified. PhaxI lyses Escherichia coli O157 : H7 in Luria-Bertani medium and milk, has an eclipse period of 20 min and a latent period of 40 min, and has a burst size of about 420 particles per cell. PhaxI is a member of the genus 'Viunalikevirus' of the family Myoviridae and is specific for E. coli O157 : H7.
authors.affiliation: Pharmaceutics Research Center, Kerman University of Medical Sciences, PO Box 76175-493, Kerman, Iran.|Department of Pharmaceutical Biotechnology and Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, PO Box 14155-6451, Tehran, Iran.
authors.forename: Salehe Sabouri
authors.initials: SS
authors.lastname: Shahrbabak
authors.affiliation: Department of Pharmaceutical Biotechnology and Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, PO Box 14155-6451, Tehran, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Khodabandehlou
authors.affiliation: Department of Pharmaceutical Biotechnology and Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, PO Box 14155-6451, Tehran, Iran.
authors.forename: Ahmad Reza
authors.initials: AR
authors.lastname: Shahverdi
authors.affiliation: Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki and Helsinki University Central Hospital Laboratory, Helsinki, Finland.
authors.forename: Mikael
authors.initials: M
authors.lastname: Skurnik
authors.affiliation: Department of Microbiology, Immunology, and Infectiology, Faculty of Medicine, Laval University, Quebec, QC; G1X 4C6, Canada.
authors.forename: Hans-Wolfgang
authors.initials: HW
authors.lastname: Ackermann
authors.affiliation: Institute of Biotechnology, PO Box 65, University of Helsinki, Helsinki, Finland.
authors.forename: Markku
authors.initials: M
authors.lastname: Varjosalo
authors.affiliation: Department of Pharmaceutical Biotechnology and Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, PO Box 14155-6451, Tehran, Iran.
authors.forename: Mojtaba Tabatabaei
authors.initials: MT
authors.lastname: Yazdi
authors.affiliation: Department of Pharmaceutical Biotechnology and Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, PO Box 14155-6451, Tehran, Iran.
authors.forename: Zargham
authors.initials: Z
authors.lastname: Sepehrizadeh
mesh_terms: D001482:Base Composition; D003090:Coliphages / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics*; D019453:Escherichia coli O157 / Q000821:virology*; D016679:Genome, Viral; D007492:Iran; D013058:Mass Spectrometry; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D017900:Myoviridae / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000648:ultrastructure; D016366:Open Reading Frames; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000821:virology; D014771:Virion / Q000737:chemistry / Q000648:ultrastructure
chemical_list: D004279:DNA, Viral; D012722:Sewage