title: High mobility group protein N5 (HMGN5) and lamina-associated polypeptide 2α (LAP2α) interact and reciprocally affect their genome-wide chromatin organization.
abstract: The interactions of nuclear lamins with the chromatin fiber play an important role in regulating nuclear architecture and chromatin function; however, the full spectrum of these interactions is not known. We report that the N-terminal domain of the nucleosome-binding protein HMGN5 interacts with the C-terminal domain of the lamin-binding protein LAP2α and that these proteins reciprocally alter their interaction with chromatin. Chromatin immunoprecipitation analysis of cells lacking either HMGN5 or LAP2α reveals that loss of either protein affects the genome-wide distribution of the remaining partner. Our study identifies a new functional link between chromatin-binding and lamin-binding proteins.
authors.affiliation: Protein Section, Laboratory of Metabolism, Center for Cancer Research, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA.
authors.forename: Shaofei
authors.initials: S
authors.lastname: Zhang
authors.forename: Dustin E
authors.initials: DE
authors.lastname: Schones
authors.forename: Cedric
authors.initials: C
authors.lastname: Malicet
authors.forename: Mark
authors.initials: M
authors.lastname: Rochman
authors.forename: Ming
authors.initials: M
authors.lastname: Zhou
authors.forename: Roland
authors.initials: R
authors.lastname: Foisner
authors.forename: Michael
authors.initials: M
authors.lastname: Bustin
mesh_terms: D001665:Binding Sites / Q000235:genetics; D015153:Blotting, Western; D002467:Cell Nucleus / Q000378:metabolism; D002843:Chromatin / Q000235:genetics / Q000378:metabolism*; D047369:Chromatin Immunoprecipitation; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D036681:Fluorescence Recovery After Photobleaching; D015894:Genome, Human; D057809:HEK293 Cells; D024001:HMGN Proteins / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D007700:Kinetics; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D011485:Protein Binding; D034622:RNA Interference; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*
chemical_list: D002843:Chromatin; D004268:DNA-Binding Proteins; D024001:HMGN Proteins; C429289:HMGN5 protein, human; D008164:Luminescent Proteins; D008565:Membrane Proteins; D015534:Trans-Activators; C093413:lamina-associated polypeptide 2
keywords: Cell Biology; Chromatin; Chromosomes/Non-histone Chromosomal Proteins; HMG Proteins; LAP2Alpha; Lamin; Nuclear Organization