title: The growth and delivery of mesenchymal and limbal stem cells using copolymer polyamide 6/12 nanofiber scaffolds.
abstract: The injured or otherwise damaged cornea is healed by limbal stem cells (LSC). If the limbus where LSC reside is also damaged or nonfunctional, the cornea cannot heal properly and this defect leads to impaired vision that can result in blindness. The only way to treat total LSC deficiency is by transplantation of limbal tissue or a transfer of LSC. Recently, mesenchymal stem cells (MSC) have been shown as another promising source of stem cells for corneal healing and regeneration. Here, we describe a protocol for the use of polyamide 6/12 nanofiber scaffolds for the growth of MSC and LSC, and for their transfer onto a mechanically damaged ocular surface in the experimental mouse model.
authors.affiliation: Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Holan
authors.forename: Eliska
authors.initials: E
authors.lastname: Javorkova
authors.forename: Peter
authors.initials: P
authors.lastname: Trosan
mesh_terms: D053687:Adult Stem Cells / Q000378:metabolism / Q000637:transplantation*; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D018929:Cell Culture Techniques; D002469:Cell Separation; D048430:Cell Shape; D002478:Cells, Cultured; D006801:Humans; D016850:Limbus Corneae / Q000166:cytology; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D057139:Nanofibers / Q000737:chemistry*; D009757:Nylons / Q000737:chemistry*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D015703:Antigens, CD; D009757:Nylons