title: Saliva improves Streptococcus mitis protective effect on human gingival fibroblasts in presence of 2-hydroxyethyl-methacrylate.
abstract: This study aimed to investigate the effect of saliva on Streptococcus mitis free cells and on S. mitis/human gingival fibroblasts (HGFs) co-culture model, in presence of 2-hydroxyethyl-methacrylate (HEMA). The bacterial aggregation both in the planktonic phase and on HGFs, as well as the apoptotic and necrotic eukaryotic cells amount were analyzed, in presence of saliva and/or HEMA. The aggregation test revealed a significant saliva aggregation effect on S. mitis strains compared to the untreated sample. No significant differences were recorded in the amount of culturable bacteria in all studied conditions; however, from microscopy images, the saliva/HEMA combining effect induced a significant bacterial aggregation and adhesion on HGFs. HEMA treatment decreased viable eukaryotic cell number with a parallel increment of necrotic cells, but when saliva was added to the co-culture, the viable cells percentage increased to a value comparable to the control sample.
authors.affiliation: Department of Pharmacy, University "G. d'Annunzio", Chieti-Pescara, Via dei Vestini, 31, Chieti, Italy.
authors.forename: Mara
authors.initials: M
authors.lastname: Di Giulio
authors.forename: Viviana
authors.initials: V
authors.lastname: di Giacomo
authors.forename: Emanuela
authors.initials: E
authors.lastname: Di Campli
authors.forename: Soraya
authors.initials: S
authors.lastname: Di Bartolomeo
authors.forename: Susi
authors.initials: S
authors.lastname: Zara
authors.forename: Guido
authors.initials: G
authors.lastname: Pasquantonio
authors.forename: Amelia
authors.initials: A
authors.lastname: Cataldi
authors.forename: Luigina
authors.initials: L
authors.lastname: Cellini
mesh_terms: D017209:Apoptosis; D001422:Bacterial Adhesion / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D018920:Coculture Techniques; D005347:Fibroblasts / Q000166:cytology / Q000382:microbiology*; D005881:Gingiva / Q000166:cytology / Q000382:microbiology*; D006801:Humans; D008689:Methacrylates / Q000494:pharmacology*; D012463:Saliva / Q000502:physiology*; D034361:Streptococcus mitis / Q000187:drug effects* / Q000502:physiology
chemical_list: D008689:Methacrylates; C005044:hydroxyethyl methacrylate