title: Label-free immunosensor for the detection of kanamycin using Ag@Fe₃O₄ nanoparticles and thionine mixed graphene sheet.
abstract: A highly sensitive label-free immunosensor for the detection of kanamycin had been developed using silver hybridized mesoporous ferroferric oxide nanoparticles (Ag@Fe₃O₄ NPs) and thionine mixed graphene sheet (TH-GS). TH was used as an electron transfer mediator. The electrical signal was greatly improved in the presence of GS due to its good electron-transfer ability. With the advantages of large specific surface area and excellent electrical conductivity, Ag@Fe₃O₄ NPs could immobilize more antibodies of kanamycin and promote the electron transfer. Cyclic voltammetry and square wave voltammetry were used to characterize the recognition of kanamycin. The proposed immunosensor showed good performances such as low detection limit (15 pg mL⁻¹), wide linear range (from 0.050 to 16 ng mL⁻¹), short analysis time (3 min), high stability, and good selectivity in the detection of kanamycin. The immunosensor was evaluated for pork meat sample, receiving satisfactory results.
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
authors.forename: Shujun
authors.initials: S
authors.lastname: Yu
authors.forename: Qin
authors.initials: Q
authors.lastname: Wei
authors.forename: Bin
authors.initials: B
authors.lastname: Du
authors.forename: Dan
authors.initials: D
authors.lastname: Wu
authors.forename: He
authors.initials: H
authors.lastname: Li
authors.forename: Liangguo
authors.initials: L
authors.lastname: Yan
authors.forename: Hongmin
authors.initials: H
authors.lastname: Ma
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D055910:Antibodies, Immobilized / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods; D055664:Electrochemical Techniques / Q000379:methods; D052203:Ferrosoferric Oxide / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D007612:Kanamycin / Q000032:analysis*; D057230:Limit of Detection; D008460:Meat / Q000032:analysis; D053758:Nanoparticles / Q000737:chemistry*; D010640:Phenothiazines / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013552:Swine
chemical_list: D000900:Anti-Bacterial Agents; D055910:Antibodies, Immobilized; D010640:Phenothiazines; D012834:Silver; D007612:Kanamycin; D006108:Graphite; C009469:thionine; D052203:Ferrosoferric Oxide