title: Comparison of the genetic background of different Colombian populations using the SNPforID 52plex identification panel.
abstract: Various strategies for analysing SNP markers and genotyping have been published with the goal of obtaining informative profiles from biological samples that contain only small amounts of template and/or degraded DNA. In this study, a multiplex assay of 52 autosomal single-nucleotide polymorphisms (SNPs) was used to analyse 438 individuals from urban populations from different regions of Colombia, as well as a sample of 50 Native American individuals of the Pastos ethnic group from Nariño. To determine if significant differences in these 52 SNPs exist between the distinct regions of Colombia, genetic distance and admixture analyses were performed based on the available data for 17 different Colombian population groups and for population groups from Africa, Europe and America. The results demonstrate significant differences between the populations from the Southwest Andean, Central-West Andean, Central-East Andean, Orinoquian and northern Colombian Pacific Coast regions. Most of the regions exhibited a European and Native American admixture. One exception is the population from the region of Chocó (on the northern Pacific Coast), which exhibits a high proportion of African admixture (54 %). From the observed genetic backgrounds, it is possible to conclude that a single reference database for the entire country would not be suitable for forensic purposes. The allele frequencies and the forensically relevant parameters were calculated for all of the markers in each Colombian region with significant values for the combined matching probability (power of discrimination ≥0.99999999999999990) and the combined probability of exclusion (≥0.9990) in trios that were obtained from all of the population groups.
authors.affiliation: IdentiGEN-Genetic Identification Laboratory and Research Group of Genetic Identification, School of Natural and Exact Sciences (FCEN), University of Antioquia, Calle 67, 53-108, Bloque 7-321, Medellin, Colombia.
authors.forename: Adriana
authors.initials: A
authors.lastname: Ibarra
authors.forename: Ana
authors.initials: A
authors.lastname: Freire-Aradas
authors.forename: Martha
authors.initials: M
authors.lastname: Martínez
authors.forename: Manuel
authors.initials: M
authors.lastname: Fondevila
authors.forename: German
authors.initials: G
authors.lastname: Burgos
authors.forename: Mauricio
authors.initials: M
authors.lastname: Camacho
authors.forename: Henry
authors.initials: H
authors.lastname: Ostos
authors.forename: Zuleyma
authors.initials: Z
authors.lastname: Suarez
authors.forename: Angel
authors.initials: A
authors.lastname: Carracedo
authors.forename: Sidney
authors.initials: S
authors.lastname: Santos
authors.forename: Leonor
authors.initials: L
authors.lastname: Gusmão
mesh_terms: D003105:Colombia; D003431:Cross-Cultural Comparison; D016172:DNA Fingerprinting / Q000379:methods*; D005006:Ethnicity / Q000235:genetics*; D053803:Forensic Genetics / Q000379:methods*; D005787:Gene Frequency; D005819:Genetic Markers / Q000235:genetics*; D014644:Genetic Variation / Q000235:genetics; D005828:Genetics, Population; D005838:Genotype; D006801:Humans; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011237:Predictive Value of Tests; D011336:Probability
chemical_list: D005819:Genetic Markers