title: Genetic diversity of HA1 domain of heammaglutinin gene of influenza A(H1N1)pdm09 in Tunisia.
abstract: We present major results concerning isolation and determination of the nucleotide sequence of hemagglutinin (HA1) of the pandemic (H1N1)pdm09 influenza viruses found in Tunisia. Amino acid analysis revealed minor amino acid changes in the antigenic or receptor-binding domains. We found mutations that were also present in 1918 pandemic virus, which includes S183P in 4 and S185T mutation in 19 of 27 viruses analyzed from 2011, while none of the 2009 viruses carried these mutations. Also two specific amino acid differences into N-glycosylation sites (N288T and N276H) were detected. The phylogenetic analysis revealed that the majority of the Tunisian isolates clustered with clade A/St. Petersburg/27/2011 viruses characterized by D97N and S185T mutations. However it also reveals a trend of 2010 strains to accumulate amino acid variation and form new phylogenetic clade with three specific amino acid substitutions: V47I, E172K and K308E.
authors.affiliation: National Influenza Centre-Tunis, Unit Virology, Microbiology Laboratory, Charles Nicolle's Hospital, Tunis, Tunisia. awatefbio@ymail.com
authors.forename: Awatef
authors.initials: A
authors.lastname: El Moussi
authors.forename: Mohamed Ali
authors.initials: MA
authors.lastname: Ben Hadj Kacem
authors.forename: Francisco
authors.initials: F
authors.lastname: Pozo
authors.forename: Juan
authors.initials: J
authors.lastname: Ledesma
authors.forename: Maria Teresa
authors.initials: MT
authors.lastname: Cuevas
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Casas
authors.forename: Amine
authors.initials: A
authors.lastname: Slim
mesh_terms: D019943:Amino Acid Substitution; D016000:Cluster Analysis; D014644:Genetic Variation; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics*; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics*; D007251:Influenza, Human / Q000821:virology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014416:Tunisia
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D012367:RNA, Viral