title: Functional characterization of Nicotiana benthamiana chromomethylase 3 in developmental programs by virus-induced gene silencing.
abstract: DNA methylation is essential for normal developmental processes and genome stability. DNA methyltransferases are key enzymes catalyzing DNA methylation. Chromomethylase (CMT) genes are specific to the plant kingdom and encode chromodomain-containing methyltransferases. However, the function of CMT genes in plants remains elusive. In this study, we isolated and characterized a CMT gene from Nicotiana benthamiana, designated NbCMT3. Alignment of the NbCMT3 amino acid sequence with other plant CMT3s showed conservation of bromo-adjacent-homology and methyltransferase catalytic domains. We investigated the expression patterns of NbCMT3 and its function in developmental programs. NbCMT3 was expressed predominately in proliferating tissues such as apical shoots and young leaves. NbCMT3 protein showed a nuclear location, which could be related to its putative cellular functions. Knocking down NbCMT3 expression by virus-induced gene silencing revealed its vital role(s) in leaf morphogenesis. The formation of palisade cells was defective in NbCMT3-silenced plants as compared with controls. NbCMT3 has a role in developmental programs.
authors.affiliation: Department of Biology, National Chunghua University of Education, No.1, Jin-De Road, 500, Changhua, Taiwan.
authors.forename: Pin-Quan
authors.initials: PQ
authors.lastname: Hou
authors.forename: Yung-I
authors.initials: YI
authors.lastname: Lee
authors.forename: Kuei-Ting
authors.initials: KT
authors.lastname: Hsu
authors.forename: Yu-Ting
authors.initials: YT
authors.lastname: Lin
authors.forename: Wei-Zhi
authors.initials: WZ
authors.lastname: Wu
authors.forename: John-Yee
authors.initials: JY
authors.lastname: Lin
authors.forename: Trinh Ngoc
authors.initials: TN
authors.lastname: Nam
authors.forename: Shih-Feng
authors.initials: SF
authors.lastname: Fu
mesh_terms: D000595:Amino Acid Sequence; D004248:DNA (Cytosine-5-)-Methyltransferases / Q000302:isolation & purification / Q000378:metabolism*; D019175:DNA Methylation; D020868:Gene Silencing; D008969:Molecular Sequence Data; D063246:Organogenesis, Plant; D010641:Phenotype; D018515:Plant Leaves / Q000166:cytology / Q000254:growth & development; D010940:Plant Proteins / Q000302:isolation & purification / Q000378:metabolism; D017422:Sequence Analysis, DNA; D014026:Nicotiana / Q000201:enzymology* / Q000254:growth & development
chemical_list: D010940:Plant Proteins; D004248:DNA (Cytosine-5-)-Methyltransferases; C112810:chromomethylase