title: Protective effect of apigenin on mouse acute liver injury induced by acetaminophen is associated with increment of hepatic glutathione reductase activity.
abstract: Apigenin, a natural plant flavone, has many beneficial effects, but there is no report about treatment of acetaminophen-induced liver injury. Our aim was to examine the protective effect of apigenin on acetaminophen-induced mouse acute liver injury and to investigate the potential mechanisms. A mouse model with acute liver injury was induced by intraperitoneally given acetaminophen 350 mg kg(-1) after oral administration of apigenin 100 and 200 mg kg(-1) for 7 days. The results showed that after treatment with apigenin, the levels of serum alanine aminotransferase and aspartate aminotransferase were gradually decreased, and the severity of liver injury was decreased. In particular, significant changes in liver necrosis were observed in the apigenin 200 mg kg(-1) group. Apigenin could gradually increase the hepatic glutathione reductase (GR) activity and reduced glutathione (GSH) content, and decrease the hepatic malondialdehyde content, but the activities of glutathione peroxidase and glutathione S-transferase in hepatic tissues between the model group and the apigenin-treated groups were not significantly different. It was concluded that apigenin could protect against acetaminophen-induced acute liver injury in mice, and the mechanisms might be associated with enhancing hepatic GSH content via increment of GR activity.
authors.affiliation: Department of Pharmacology, College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, Jiangsu Province, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Yang
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Wang
authors.forename: Jie
authors.initials: J
authors.lastname: Xue
authors.forename: Zhen-Lun
authors.initials: ZL
authors.lastname: Gu
authors.forename: Mei-Lin
authors.initials: ML
authors.lastname: Xie
mesh_terms: D000082:Acetaminophen / Q000009:adverse effects; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D047310:Apigenin / Q000008:administration & dosage*; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy / Q000201:enzymology / Q000209:etiology / Q000517:prevention & control*; D005980:Glutathione Reductase / Q000235:genetics / Q000378:metabolism*; D005982:Glutathione Transferase / Q000097:blood; D006801:Humans; D008099:Liver / Q000187:drug effects / Q000201:enzymology* / Q000293:injuries / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D020011:Protective Agents / Q000008:administration & dosage*
chemical_list: D020011:Protective Agents; D000082:Acetaminophen; D008315:Malondialdehyde; D047310:Apigenin; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D000410:Alanine Transaminase