title: In vitro effects of bisphenol A on the quality parameters, oxidative stress, DNA integrity and adenosine triphosphate content in sterlet (Acipenser ruthenus) spermatozoa.
abstract: Among endocrine disruptors, the xenoestrogen bisphenol A (BPA) deserves particular attention due to widespread human exposure. Besides hormonal effects, BPA has been suspected to be responsible for adverse effect on reproductive ability of various species. In the present study the effect of BPA on the quality parameters, oxidative stress, the DNA integrity and intracellular ATP content of sterlet (Acipenser ruthenus) spermatozoa were investigated in vitro. Fish spermatozoa were exposed to concentrations of BPA possibly occurring in nature (0.5, 1.75, 2.5, 5 and 10μg/L) for 2h. Results revealed that BPA significantly decreased spermatozoa motility and velocity of spermatozoa at concentration of BPA 2.5-10μg/L. Significant positive correlation (r=0.713, P<0.05) was found between percent motile spermatozoa and ATP content. Oxidative stress was observed at concentrations 1.75-10μg/L, as reflected by significantly higher levels of protein and lipid oxidation and superoxide dismutase activity. Intracellular ATP content of spermatozoa decreased with increasing concentrations of BPA. A dramatic increase in DNA fragmentation expressed as percent tail DNA (2.2%±0.46) and Olive tail moment (0.37±0.09 arbitrary units) was recorded at concentrations of 1.75μg/L and above. The present study confirms that concentrations of BPA that can be encountered in nature are capable to induce oxidative stress, leading to impaired sperm quality, DNA fragmentation and intracellular ATP content.
authors.affiliation: Faculty of Fisheries and Protection of Waters, Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia, Zatisi 728/II, Vodnany, Czech Republic.
authors.forename: Martin
authors.initials: M
authors.lastname: Hulak
authors.forename: Ievgeniia
authors.initials: I
authors.lastname: Gazo
authors.forename: Anna
authors.initials: A
authors.lastname: Shaliutina
authors.forename: Pavla
authors.initials: P
authors.lastname: Linhartova
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001559:Benzhydryl Compounds / Q000633:toxicity*; D004247:DNA / Q000235:genetics; D053938:DNA Fragmentation / Q000187:drug effects; D052244:Endocrine Disruptors / Q000633:toxicity*; D005399:Fishes / Q000235:genetics*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000633:toxicity*; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000378:metabolism; D000098826:Bisphenol A Compounds
chemical_list: D000255:Adenosine Triphosphate; D000975:Antioxidants; D001559:Benzhydryl Compounds; D004247:DNA; D052244:Endocrine Disruptors; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds