title: [The advance in synthetic biology: towards a microbe-derived paclitaxel intermediates].
abstract: The synthetic biology matures to promote the heterologous biosynthesis of the well-known drug paclitaxel that is one of the most important and active chemotherapeutic agents for the first-line clinical treatment of cancer. This review focuses on the construction and regulation of the biosynthetic pathway of paclitaxel intermediates in both Escherichia coli and Saccharomyces cerevisiae. In particular, the review also features the early efforts to design and overproduce taxadiene and the bottleneck of scale fermentation for producing the intermediates.
authors.affiliation: State Key Laboratory of Bioactive Substances and Functions of Natural Medicines & Key Laboratory of Biosynthesis of Natural Products, Ministry of Health of PRC, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China. wwang@imm.ac.cn
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Zheng
authors.forename: Shu-Qiong
authors.initials: SQ
authors.lastname: Huang
authors.forename: Lei
authors.initials: L
authors.lastname: Guo
authors.forename: Jian-Qiang
authors.initials: JQ
authors.lastname: Kong
authors.forename: Ke-Di
authors.initials: KD
authors.lastname: Cheng
mesh_terms: D000475:Alkenes / Q000737:chemistry / Q000378:metabolism*; D000972:Antineoplastic Agents, Phytogenic / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism; D053898:Biosynthetic Pathways; D004224:Diterpenes / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*; D005285:Fermentation; D060847:Metabolic Engineering; D017239:Paclitaxel / Q000096:biosynthesis* / Q000737:chemistry / Q000378:metabolism; D011355:Prodrugs; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D058615:Synthetic Biology
chemical_list: D000475:Alkenes; D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; D011355:Prodrugs; C093125:taxa-4(5),11(12)diene; D017239:Paclitaxel