title: NSP1 is a component of the Myc signaling pathway.
abstract: Nodulation and arbuscular mycorrhization require the activation of plant host symbiotic programs by Nod factors, and Myc-LCOs and COs, respectively. The pathways involved in the perception and downstream signaling of these signals include common and distinct components. Among the distinct components, NSP1, a GRAS transcription factor, has been considered for years to be specifically involved in nodulation. Here, we analyzed the degree of conservation of the NSP1 sequence in arbuscular mycorrhizal (AM) host and non-AM host plants and carefully examined the ability of Medicago truncatula nsp1 mutants to respond to Myc-LCOs and to be colonized by an arbuscular mycorrhizal fungus. In AM-host plants, the selection pressure on NSP1 is stronger than in non-AM host ones. The response to Myc-LCOs and the frequency of mycorrhizal colonization are significantly reduced in the nsp1 mutants. Our results reveal that NSP1, previously described for its involvement in the Nod factor signaling pathway, is also involved in the Myc-LCO signaling pathway. They bring additional evidence on the evolutionary relatedness between nodulation and mycorrhization.
authors.affiliation: Department of Agronomy, University of Wisconsin Madison, 219 Moore Hall, 1575 Linden Drive, Madison, WI, 53706, USA.
authors.forename: Pierre-Marc
authors.initials: PM
authors.lastname: Delaux
authors.affiliation: Laboratoire de Recherche en Sciences Végétales, UMR5546, Université de Toulouse, 31326, Castanet-Tolosan CEDEX, France.|CNRS, UMR5546, 31326, Castanet-Tolosan CEDEX, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Bécard
authors.affiliation: Laboratoire de Recherche en Sciences Végétales, UMR5546, Université de Toulouse, 31326, Castanet-Tolosan CEDEX, France.|CNRS, UMR5546, 31326, Castanet-Tolosan CEDEX, France.
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Combier
mesh_terms: D018506:Gene Expression Regulation, Plant; D046913:Medicago truncatula / Q000235:genetics / Q000378:metabolism / Q000382:microbiology; D009154:Mutation; D038821:Mycorrhizae / Q000502:physiology*; D009844:Oligosaccharides / Q000378:metabolism*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000382:microbiology; D015398:Signal Transduction; D013559:Symbiosis; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D009844:Oligosaccharides; D010940:Plant Proteins; D014157:Transcription Factors