title: Integration of a graphite/poly(methyl-methacrylate) composite electrode into a poly(methylmethacrylate) substrate for electrochemical detection in microchips.
abstract: Traditional fabrication methods for polymer microchips, the bonding of two substrates together to form the microchip, can make the integration of carbon electrodes difficult. We have developed a simple and inexpensive method to integrate graphite/PMMA composite electrodes (GPCEs) into a PMMA substrate. These substrates can be bonded to other PMMA layers using a solvent-assisted thermal bonding method. The optimal composition of the GPCEs for electrochemical detection was determined using cyclic voltammetry with dopamine as a test analyte. Using the optimized GPCEs in an all-PMMA flow cell with flow injection analysis, it was possible to detect 50 nM dopamine under the best conditions. These electrodes were also evaluated for the detection of dopamine and catechol following separation by MCE.
authors.affiliation: Ralph N. Adams Institute for Bioanalytical Chemistry, University of Kansas, Lawrence, KS, USA.
authors.forename: Anne
authors.initials: A
authors.lastname: Regel
authors.forename: Susan
authors.initials: S
authors.lastname: Lunte
mesh_terms: D002244:Carbon / Q000737:chemistry; D002396:Catechols / Q000032:analysis*; D004298:Dopamine / Q000032:analysis*; D004566:Electrodes; D046209:Electrophoresis, Microchip / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D057230:Limit of Detection; D019904:Polymethyl Methacrylate / Q000737:chemistry*
chemical_list: D002396:Catechols; D002244:Carbon; D006108:Graphite; D019904:Polymethyl Methacrylate; C034221:catechol; D004298:Dopamine
keywords: Carbon electrode; Carbon paste electrode; Graphite/poly(methylmethacrylate) composite electrode; Microchip electrophoresis; Polymer microchip