title: The zebrafish CreZoo: an easy-to-handle database for novel CreER(T2)-driver lines.
abstract: We report a new open access database, the zebrafish CreZoo ( http://crezoo.crt-dresden.de ), which contains novel CreER(T2)-driver lines that express Cre fused to the mutated human ligand-binding domain of the estrogen receptor (CreER(T2)) in several tissues. Recently, the conditional Cre/loxP technology has been added to the toolbox for the precise manipulation of the zebrafish genome, but currently the number of CreER(T2)-driver lines is limited. To enlarge the pool of existing CreER(T2)-driver lines, we conducted a genome-wide screen using a gene trap cassette comprising a splice acceptor and an mCherry-tagged variant of CreER(T2). All molecular and expression data obtained in this screen are summarized in the CreZoo database, which currently comprises an inventory of about 47 Cre-driver lines expressing CreER(T2) in a cell- and tissue-specific manner during development and adulthood. Combined with other Cre-dependent effector lines, the CreZoo will be a great tool to manipulate the zebrafish genome.
authors.affiliation: Dresden University of Technology, Dresden, Germany.
authors.forename: Peggy
authors.initials: P
authors.lastname: Jungke
authors.forename: Stefan
authors.initials: S
authors.lastname: Hans
authors.forename: Michael
authors.initials: M
authors.lastname: Brand
mesh_terms: D000818:Animals; D019992:Databases as Topic; D015027:Zebrafish