title: Single-cell force spectroscopy of probiotic bacteria.
abstract: Single-cell force spectroscopy is a powerful atomic force microscopy modality in which a single living cell is attached to the atomic force microscopy cantilever to quantify the forces that drive cell-cell and cell-substrate interactions. Although various single-cell force spectroscopy protocols are well established for animal cells, application of the method to individual bacterial cells remains challenging, mainly owing to the lack of appropriate methods for the controlled attachment of single live cells on cantilevers. We present a nondestructive protocol for single-bacterial cell force spectroscopy, which combines the use of colloidal probe cantilevers and of a bioinspired polydopamine wet adhesive. Living cells from the probiotic species Lactobacillus plantarum are picked up with a polydopamine-coated colloidal probe, enabling us to quantify the adhesion forces between single bacteria and biotic (lectin monolayer) or abiotic (hydrophobic monolayer) surfaces. These minimally invasive single-cell experiments provide novel, to our knowledge, insight into the specific and nonspecific forces driving the adhesion of L. plantarum, and represent a generic platform for studying the molecular mechanisms of cell adhesion in probiotic and pathogenic bacteria.
authors.affiliation: Université catholique de Louvain, Institute of Life Sciences, Louvain-la-Neuve, Belgium.
authors.forename: Audrey
authors.initials: A
authors.lastname: Beaussart
authors.forename: Sofiane
authors.initials: S
authors.lastname: El-Kirat-Chatel
authors.forename: Philippe
authors.initials: P
authors.lastname: Herman
authors.forename: David
authors.initials: D
authors.lastname: Alsteens
authors.forename: Jacques
authors.initials: J
authors.lastname: Mahillon
authors.forename: Pascal
authors.initials: P
authors.lastname: Hols
authors.forename: Yves F
authors.initials: YF
authors.lastname: Dufrêne
mesh_terms: D048191:Lactiplantibacillus plantarum / Q000648:ultrastructure*; D018625:Microscopy, Atomic Force / Q000379:methods*; D019936:Probiotics; D059010:Single-Cell Analysis / Q000379:methods