title: A comparative study on the bulk adhesive strength of the recombinant mussel adhesive protein fp-3.
abstract: Mussel adhesive protein (MAP) type 3 (fp-3) is considered one of the key components for mussel adhesion. However, its bulk adhesive strength has not been characterized due to its availability in limited quantities. In the present work, a feasible production (~47 mg l(-1)) of recombinant fp-3 was achieved, and its bulk adhesive strength was measured for the first time; ~0.57 MPa for the unmodified form and ~0.94 and ~2.28 MPa for the 3,4-dihydroxy-L-phenylalanine (DOPA)-modified form, having a 9.6% yield without and with oxidant treatment, respectively. Furthermore, values for the bulk adhesive strength of several DOPA-modified recombinant MAPs were compared. The maximum adhesive strength of DOPA-modified fp-3 after oxidant treatment was stronger than that of type 5 (fp-5), which has a 6.2% modification yield, and was comparable to that of hybrid types fp-131 and fp-151, which have similar yields (~5%). The strong bulk adhesive property of recombinant fp-3 demonstrates its potential use as a promising bioadhesive.
authors.affiliation: Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, Korea.
authors.forename: Byeongseon
authors.initials: B
authors.lastname: Yang
authors.forename: Dong Gyun
authors.initials: DG
authors.lastname: Kang
authors.forename: Jeong Hyun
authors.initials: JH
authors.lastname: Seo
authors.forename: Yoo Seong
authors.initials: YS
authors.lastname: Choi
authors.forename: Hyung Joon
authors.initials: HJ
authors.lastname: Cha
mesh_terms: D000269:Adhesives / Q000737:chemistry; D000818:Animals; D057219:Biofouling; D001696:Biomechanical Phenomena; D049872:Bivalvia / Q000737:chemistry*; D004295:Dihydroxyphenylalanine / Q000737:chemistry / Q000378:metabolism; D008962:Models, Theoretical; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D000269:Adhesives; D011506:Proteins; D011994:Recombinant Proteins; C037403:adhesive protein, mussel; D004295:Dihydroxyphenylalanine