title: Proteomic analysis of differentially expressed proteins in lung cancer in Wistar rats using NNK as an inducer.
abstract: Lung cancer is one of the commonest cancers detected worldwide with a high mortality rate. The responsible factors affecting survival include delayed prognosis, and lack of effective treatments. To help improve the disease management, there is a need for better screening and development of specific markers that help in the early diagnosis. Analysis of differentially expressed proteins in cancer cells in comparison to their normal counterparts using proteome profiling revealed identification of new biomarkers as therapeutic targets. Therefore, an animal model for lung cancer was developed and monitored by histopathological evaluation. Lung tissue proteins were isolated, solubilized and resolved on 2D gel electrophoresis using broad pH range IPG strips (pH 3-10). Liquid chromatography and mass spectrometry (LC-MS/MS) revealed 66 proteins to be differentially expressed in cancer tissue as compared to normal. The study identified and characterized three of these proteins, namely peroxiredoxin-6, β-actin and collagen α-1 (VI) as potentially prospective biomarkers for early detection of lung cancer.
authors.affiliation: Department of Biotechnology, Jamia Hamdard, New Delhi 110062, India.
authors.forename: Naveen
authors.initials: N
authors.lastname: Chaudhary
authors.forename: Shilpa
authors.initials: S
authors.lastname: Bhatnagar
authors.forename: Shabnam
authors.initials: S
authors.lastname: Malik
authors.forename: Deepshikha P
authors.initials: DP
authors.lastname: Katare
authors.forename: S K
authors.initials: SK
authors.lastname: Jain
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002273:Carcinogens / Q000494:pharmacology; D002853:Chromatography, Liquid; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D008297:Male; D013058:Mass Spectrometry; D009602:Nitrosamines / Q000494:pharmacology*; D054465:Peroxiredoxin VI / Q000378:metabolism; D040081:Protein Array Analysis; D040901:Proteomics; D051381:Rats; D017208:Rats, Wistar; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D002273:Carcinogens; D009602:Nitrosamines; C016583:4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone; D054465:Peroxiredoxin VI