title: Selection and characterization of a novel photoperiod-sensitive male sterile line in upland cotton.
abstract: Upland cotton (Gossypium hirsutum L.) shows strong heterosis. However, heterosis is not widely utilized owing to the high cost of hybrid seed production. Creation of a photoperiod-sensitive genetic male sterile line could substantially reduce the cost of hybrid seed production in upland cotton. Such a mutant with virescent marker was found by space mutation in near-earth orbit and its traits had been stable after 4 years of selection in Anyang and Sanya, China. This mutant was fertile with an 11-12.5 h photoperiod when the temperature was higher than 21.5 °C and was sterile with a 13-14.5 h photoperiod. Genetic analysis indicated that both traits were controlled by a single recessive gene or two closely linked genes. Also, the cytological observations and transcriptome profiling analysis showed that the degradation of pollen grain cytoplasm should be the primary reason why the mutant line were male sterile under long-day conditions.
authors.affiliation: State Key Laboratory of Cotton Biology, the Institute of Cotton Research, the Chinese Academy of Agricultural Sciences, Anyang, 455000, China.
authors.forename: Jianhui
authors.initials: J
authors.lastname: Ma
authors.forename: Hengling
authors.initials: H
authors.lastname: Wei
authors.forename: Ji
authors.initials: J
authors.lastname: Liu
authors.forename: Meizhen
authors.initials: M
authors.lastname: Song
authors.forename: Chaoyou
authors.initials: C
authors.lastname: Pang
authors.forename: Long
authors.initials: L
authors.lastname: Wang
authors.forename: Wenxiang
authors.initials: W
authors.lastname: Zhang
authors.forename: Shuli
authors.initials: S
authors.lastname: Fan
authors.forename: Shuxun
authors.initials: S
authors.lastname: Yu
mesh_terms: D002734:Chlorophyll / Q000378:metabolism; D020090:Chromosome Segregation / Q000235:genetics; D003433:Crosses, Genetic; D060146:Ecotype; D035264:Flowers / Q000166:cytology / Q000254:growth & development; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D063990:Gene Ontology; D005819:Genetic Markers; D003368:Gossypium / Q000235:genetics / Q000502:physiology*; D009154:Mutation / Q000235:genetics; D017440:Photoperiod; D051479:Plant Infertility / Q000502:physiology*; D018515:Plant Leaves / Q000502:physiology; D015203:Reproducibility of Results; D017422:Sequence Analysis, DNA; D013696:Temperature; D015854:Up-Regulation / Q000235:genetics
chemical_list: D005819:Genetic Markers; D002734:Chlorophyll
keywords: Photoperiod-sensitive genetic male sterile; sterility mechanism; upland cotton; virescent marker