title: Characterization of pyrene degradation by Pseudomonas sp. strain Jpyr-1 isolated from active sewage sludge.
abstract: Using pyrene as a sole carbon, a new polycyclic aromatic hydrocarbons (PAHs)-degrading bacterial strain was isolated from the active sewage sludge. This strain was identified as Pseudomonas sp. Jpyr-1 by 16S rRNA gene sequence analysis. The maximum degradation rate of pyrene was 3.07 mg L(-1)h(-1) in 48 h incubation with initial pyrene concentration of 200 mg L(-1). Moreover, in binary system consisting of pyrene and another PAH, the enzyme system of Jpyr-1 showed a preference toward pyrene. Furthermore, competitive inhibition of pyrene degradation by other PAH compounds occurred in the binary system. Jpyr-1 could also rapidly degrade other PAHs, such as benzanthracene, chrysene and benzo[a]pyrene. Moreover, several metabolites were detected during pyrene degradation which indicated that Jpyr-1 degraded pyrene through the o-phthalate pathway. Taken together, these results indicated that Pseudomonas sp. Jpyr-1 was a new PAHs-degrading strain that might be useful in the bioremediation of sites contaminated with PAHs.
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, No. 2 Linggong Road, Ganjingzi District, Dalian, Liaoning 116023, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Ma
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.forename: Lingyun
authors.initials: L
authors.lastname: Jia
mesh_terms: D001673:Biodegradation, Environmental; D001711:Biotransformation; D008969:Molecular Sequence Data; D010802:Phylogeny; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism; D011549:Pseudomonas / Q000302:isolation & purification* / Q000378:metabolism*; D011721:Pyrenes / Q000378:metabolism*; D012722:Sewage / Q000382:microbiology*; D013379:Substrate Specificity
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes; D012722:Sewage; C030984:pyrene