title: Comparison of restriction enzymes for pulsed-field gel electrophoresis typing of Moraxella catarrhalis.
abstract: NotI, the most prevalent restriction enzyme used for typing Moraxella catarrhalis, failed to digest genomic DNA from respiratory samples. An improved pulsed-field gel electrophoresis (PFGE) methodology determined SpeI as the best choice for typing this bacterial species, with a good restriction of clinical samples and a good clustering correlation with NotI.
authors.affiliation: Microbiology Department, Hospital Universitari Bellvitge, IDIBELL, University of Barcelona, Barcelona, Spain. smartinm@bellvitgehospital.cat
authors.forename: Sara
authors.initials: S
authors.lastname: Marti
authors.forename: Carmen
authors.initials: C
authors.lastname: Puig
authors.forename: Arnau
authors.initials: A
authors.lastname: Domenech
authors.forename: Josefina
authors.initials: J
authors.lastname: Liñares
authors.forename: Carmen
authors.initials: C
authors.lastname: Ardanuy
mesh_terms: D016000:Cluster Analysis; D004262:DNA Restriction Enzymes; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D006801:Humans; D017720:Molecular Epidemiology / Q000379:methods; D058889:Molecular Typing / Q000379:methods*; D001936:Moraxella catarrhalis / Q000145:classification* / Q000235:genetics*
chemical_list: D004262:DNA Restriction Enzymes