title: Raman microspectroscopy as a diagnostic tool to study single living nasopharyngeal carcinoma cell lines.
abstract: Raman spectroscopy can provide molecular-level fingerprint information about the biochemical composition and structure of cells and tissues with excellent spatial resolution. In this study, Raman spectroscopy of 3 different nasopharyngeal carcinoma cell lines C666-1, CNE1, and CNE2 and 1 nasopharyngeal normal cell line NP69 acquired on a piece of silica glass slide are presented to investigate the differences among them. The results show the ratio of I1657/I1449 (= 0.7) could provide good distinction between tumor and normal cell lines very easily, which coincides with existing reports about the study of different cell lines and bronchial tissue. In addition, several statistical analytical methods were used to classify these 4 different cell lines and then achieved an exciting result with great sensitivity and specificity of >90%, respectively. The findings of this work further support former work where cells' Raman spectra were acquired on a different substrate. All of these results indicate Raman spectroscopy has the potential to discriminate between normal and tumor cells and have potential use in early diagnosis of nasopharyngeal carcinoma.
authors.affiliation: Fujian Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian, China.
authors.forename: Zuanfang
authors.initials: Z
authors.lastname: Li
authors.forename: Yang
authors.initials: Y
authors.lastname: Chen
authors.forename: Yongzeng
authors.initials: Y
authors.lastname: Li
authors.forename: Weiwei
authors.initials: W
authors.lastname: Chen
authors.forename: Jianji
authors.initials: J
authors.lastname: Pan
authors.forename: Ying
authors.initials: Y
authors.lastname: Su
authors.forename: Changyan
authors.initials: C
authors.lastname: Zou
mesh_terms: D045744:Cell Line, Tumor; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000473:pathology*; D013059:Spectrum Analysis, Raman / Q000379:methods*