title: [Expression of epithelial-mesenchymal transition markers in tumor tissues generated by side population and non-side population of A431 cells in NOD/SCID mice].
abstract: OBJECTIVE
  To isolate the side population (SP) and non-side population (NSP) cells from A431 cells and compare their difference in tumorigenicity in mice and the expression profiles of epithelial-mesenchymal transition (EMT) markers.
  
  
  METHODS
  A431 cells stained with Hoechst 33342 were sorted with flow cytometry. The isolated SP cells and NSP cells were inoculated into NOD/SCID mice and the tumorigenicity of the cells was observed. EMT markers E-cadherin, β-catenin, vimentin, AXL, and Erbb3 in the tumor tissues were detected by immunohisto-staining.
  
  
  RESULTS
  The tumors generated by SP cells were larger than those by NSP cells in NOD/SCID mice. Compared with the tumors generated by NSP cells, the cells in the periphery of tumors generated by SP cells showed up-regulated expressions of AXL, vimentin and β-catenin and down-regulated ERBB3 and E-cadherin.
  
  
  CONCLUSIONS
  The SP cells in A431 cells have a strong tumorigenicity and show more EMT phenotypes in tissues.
authors.affiliation: Department of Immunology and Pathobiology, Xi'an Medical College, Xi'an, China. shliqi0220@163.com
authors.forename: Lingqiao
authors.initials: L
authors.lastname: Shao
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Wang
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Guo
authors.forename: Songmei
authors.initials: S
authors.lastname: Geng
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D058750:Epithelial-Mesenchymal Transition; D006801:Humans; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D014411:Neoplastic Stem Cells / Q000378:metabolism; D058985:Side-Population Cells / Q000378:metabolism*; D012878:Skin Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor