title: The antioxidant activity of copper(II) (3,5-diisopropyl salicylate)4 and its protective effect against streptozotocin-induced diabetes mellitus in rats.
abstract: Oxidative stress has been suggested as a potential contributor to the development of diabetic complications. In this study, we investigated the protective effect of a strong antioxidant copper complex against streptozotocin (STZ)-induced diabetes in animals. Out of four copper complexes used, copper(II) (3,5-diisopropyl salicylate)4 (Cu(II)DIPS) was found to be the most potent antioxidant-copper complex. Pretreatment with Cu(II)DIPS (5 mg/kg) twice a week prior to the injection of streptozotocin (50 mg/kg) has reduced the level of hyperglycemia by 34 % and the mortality rate by 29 %. Injection of the same dosage of the ligand 3,5-diisopropyl salicylate has no effect on streptozotocin-induced hyperglycemia. The same copper complex has neither hypoglycemic activity when injected in normal rats nor antidiabetic activity when injected in STZ-induced diabetic rats. The protective effect of Cu(II)DIPS could be related to its strong antioxidant activity compared to other copper complexes median effective concentration (MEC) = 23.84 μg/ml and to Trolox MEC = 29.30 μg/ml. In addition, it reduced serum 8-hydroxy-2'-deoxyguanosine, a biomarker of oxidative DNA damage, by 29 %. This effect may explain why it was not effective against diabetic rats, when β Langerhans cells were already destroyed. Similar protective activities were reported by other antioxidants like Trolox.
authors.affiliation: Faculty of Nursing and Allied Health Professions, Birzeit University, Birzeit, Palestine. mqazzaz@birzeit.edu
authors.forename: Munir
authors.initials: M
authors.lastname: Qazzaz
authors.forename: Rula
authors.initials: R
authors.lastname: Abdul-Ghani
authors.forename: Munther
authors.initials: M
authors.lastname: Metani
authors.forename: Rateb
authors.initials: R
authors.lastname: Husein
authors.forename: Abdul-Latif
authors.initials: AL
authors.lastname: Abu-Hijleh
authors.forename: Abdul-Salam
authors.initials: AS
authors.lastname: Abdul-Ghani
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D001786:Blood Glucose; D002839:Chromans / Q000627:therapeutic use; D056831:Coordination Complexes / Q000627:therapeutic use; D003300:Copper / Q000737:chemistry*; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012459:Salicylates / Q000737:chemistry / Q000627:therapeutic use*
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D002839:Chromans; D056831:Coordination Complexes; D012459:Salicylates; C030865:copper bis(3,5-diisopropylsalicylate); D003300:Copper; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid