title: Association of aldosterone synthase (CYP11B2) gene -344T/C polymorphism with the risk of primary chronic glomerulonephritis in the Polish population.
abstract: BACKGROUND
  We evaluate whether angiotensinogen AGT M235T (rs699), angiotensin-converting enzyme ACE (I/D) (rs4646994) and aldosterone synthase CYP11B2 -344C/T (rs1799998) polymorphisms can be genetic risk factors of chronic glomerulonephritis (GN) in the Polish population.
  
  
  METHODS
  The study was conducted in 140 patients with primary chronic GN: mesangial proliferative GN (MesPGN) (n = 49), IgA nephropathy (IgAN) (n = 31), membranous nephropathy (MN) (n = 27), focal segmental glomerulosclerosis (FSGS) (n = 25), membranoproliferative GN (MPGN) (n = 4), and minimal change disease (MCD) (n = 4), and controls (n = 187). Genotypes were determined by HRM curve analysis for AGT M235T, by PCR and agarose gel separation for ACE (I/D), and by PCR-RFLP for CYP11B2 -344C/T.
  
  
  RESULTS
  We found a significant association of the CYP11B2 -344C/T polymorphism in the recessive model with all subtypes of GN (OR = 1.925 (95% CI = 1.152-3.219, p = 0.0118, p(corr) = 0.0354)). We also observed that the CYP11B2 -344C/T polymorphism in the recessive model may also be an independent significant risk factor of IgAN (OR = 2.743 (95% CI = 1.219-6.172, p = 0.0122, p(corr) = 0.0366)), FSGS (OR = 2.895 (95% CI = 1.200-6.985, p = 0.0145, p(corr) = 0.0435)), and all proliferative GNs (MesPGN, IgAN, MPGN) (OR = 2.171 (95% CI = 1.211-3.894, p = 0.0084, p(corr) = 0.0252)).
  
  
  CONCLUSIONS
  Our results suggest that the CYP11B2 -344C/T polymorphism might be an independent risk factor of IgAN, FSGS and all proliferative chronic GNs.
authors.affiliation: Department of Nephrology, Transplantology and Internal Medicine.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Pawlik
authors.affiliation: Department of Biochemistry and Molecular Biology, Poznan University of Medical Sciences, Poland.
authors.forename: Adrianna
authors.initials: A
authors.lastname: Mostowska
authors.affiliation: Department of Biochemistry and Molecular Biology, Poznan University of Medical Sciences, Poland.
authors.forename: Margarita
authors.initials: M
authors.lastname: Lianeri
authors.affiliation: Department of Nephrology, Transplantology and Internal Medicine.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Oko
authors.affiliation: Department of Biochemistry and Molecular Biology, Poznan University of Medical Sciences, Poland pjagodzi@am.poznan.pl.
authors.forename: Paweł P
authors.initials: PP
authors.lastname: Jagodziński
mesh_terms: D000328:Adult; D000368:Aged; D002908:Chronic Disease; D019405:Cytochrome P-450 CYP11B2 / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005921:Glomerulonephritis / Q000201:enzymology* / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011044:Poland; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012307:Risk Factors; D055815:Young Adult
chemical_list: D019405:Cytochrome P-450 CYP11B2
keywords: CYP11B2; glomerulonephritis; polymorphisms