title: Repeated supra-maximal sprint cycling with and without sodium bicarbonate supplementation induces endothelial microparticle release.
abstract: Under normal homeostatic conditions, the endothelium releases microparticles (MPs), which are known to increase under stressful conditions and in disease states. CD105 (endoglin) and CD106 (vascular cell adhesion molecule-1) are expressed on the surface of endothelial cells and increased expression in response to stress may be observed. A randomised-controlled double-blinded study aimed to examine the use of endothelial MPs as a marker for the state of one's endothelium, as well as whether maintaining acid-base homeostasis affects the release of these MPs. This study tested seven healthy male volunteers, who completed a strenuous cycling protocol, with venous blood analysed for CD105+ and CD106+ MPs by flow cytometry at regular intervals. Prior to each trial participants consumed either 0.3 g·kg(-1) body mass of sodium bicarbonate (NaHCO3), or 0.045 g·kg(-1) body mass of sodium chloride (NaCl). A significant rise in endothelial CD105+ MPs and CD106+ MPs (p<0.05) was observed at 90 min post-exercise. A significant trend was shown for these MPs to return to resting levels 180 min post-exercise in both groups. No significance was found between experimental groups, suggesting that maintaining acid-base variables closer to basal levels has little effect upon the endothelial stress response for this particular exercise mode. In conclusion, strenuous exercise is accompanied by MP release and the endothelium is able to rapidly recover in healthy individuals, whilst maintaining acid-base homeostasis does not attenuate the MP release from the endothelium after exercise.
authors.affiliation: a Department of Sport, Health and Exercise Science , University of Hull , Hull , UK.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Kirk
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Peart
authors.forename: Leigh A
authors.initials: LA
authors.lastname: Madden
authors.forename: Rebecca V
authors.initials: RV
authors.lastname: Vince
mesh_terms: D000293:Adolescent; D000328:Adult; D015703:Antigens, CD / Q000097:blood*; D001642:Bicycling / Q000502:physiology*; D055252:Cell-Derived Microparticles / Q000378:metabolism; D019587:Dietary Supplements; D004311:Double-Blind Method; D000071063:Endoglin; D042783:Endothelial Cells / Q000187:drug effects*; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D011956:Receptors, Cell Surface / Q000097:blood*; D017693:Sodium Bicarbonate / Q000494:pharmacology*; D019010:Vascular Cell Adhesion Molecule-1 / Q000097:blood*; D055815:Young Adult
chemical_list: D015703:Antigens, CD; C064206:ENG protein, human; D000071063:Endoglin; D011956:Receptors, Cell Surface; D019010:Vascular Cell Adhesion Molecule-1; D017693:Sodium Bicarbonate
keywords: Endothelial function; anaerobic exercise; microparticles; sodium bicarbonate; vascular cell adhesion molecule-1