title: Metabolic engineering of Candida utilis for isopropanol production.
abstract: A genetically-engineered strain of the yeast Candida utilis harboring genes encoding (1) an acetoacetyl-CoA transferase from Clostridium acetobutylicum ATCC 824, (2) an acetoacetate decarboxylase, and (3) a primary-secondary alcohol dehydrogenase derived from Clostridium beijerinckii NRRL B593 produced up to 0.21 g/L of isopropanol. Because the engineered strain accumulated acetate, isopropanol titer was improved to 1.2 g/L under neutralized fermentation conditions. Optimization of isopropanol production was attempted by the overexpression and disruption of several endogenous genes. Simultaneous overexpression of two genes encoding acetyl-CoA synthetase and acetyl-CoA acetyltransferase increased isopropanol titer to 9.5 g/L. Moreover, in fed-batch cultivation, the resultant recombinant strain produced 27.2 g/L of isopropanol from glucose with a yield of 41.5 % (mol/mol). This is the first demonstration of the production of isopropanol by genetically engineered yeast.
authors.affiliation: Central Laboratories for Key Technologies, KIRIN Company Ltd., 1-13-5 Fukuura Kanazawa-ku, Yokohama, Kanagawa 236-0004, Japan. Hideyuki_Tamakawa@kirin.co.jp
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Tamakawa
authors.forename: Tokiko
authors.initials: T
authors.lastname: Mita
authors.forename: Aki
authors.initials: A
authors.lastname: Yokoyama
authors.forename: Shigehito
authors.initials: S
authors.lastname: Ikushima
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yoshida
mesh_terms: D019840:2-Propanol / Q000378:metabolism*; D000085:Acetates / Q000378:metabolism; D002175:Candida / Q000235:genetics* / Q000378:metabolism*; D005285:Fermentation; D007218:Industrial Microbiology; D060847:Metabolic Engineering
chemical_list: D000085:Acetates; D019840:2-Propanol