title: [Chemical constituents and their antitumor cytotoxic activity in leaves of Ilex centrochinensis].
abstract: OBJECTIVE
  To investigate the chemical constituents in leaves of Ilex centrochinensis and their antitumor bioactivity.
  
  
  METHODS
  Various chromatography techniques such as column chromatography on silica gel, Sephadex LH-20 and preparative HPLC were used to isolate and purify the compounds and their structures were identified by spectral data and physicochemical properties. Their antitumor effect was tested by MTT method.
  
  
  RESULTS
  Ten compounds were isolated and identified as 1,4-benzenediol (1), (2S)-5,4'-dihydroxy-7,3'-dimethoxyflavan(2), (2S)-5,4'-dihydroxy-7-methoxyflavan (3), kaempferol (4), quercetin (5), naringenin (6), ursolic acid (7), uvaol (8), oleanolic acid (9) and beta-sitosterols (10).
  
  
  CONCLUSIONS
  Compounds 1-5, 7, 8 were isolated from the species for the first time, among which compounds 1-3 were isolated from the Ilex genus for the first time. Compounds 2 and 3 showed strong cytotoxic activity against Huh7 cell lines with IC50 values of 8.98, 13.04 mg x L(-1), respectively. Compounds 7-9 exhibited weak cytotoxic activity against Caco-2 cell lines with IC50 values of 28.52, 38.28, 33.04 mg x L(-1), respectively.
authors.affiliation: The Second Clinical Medical College of Jinan University, Shenzhen Institute of Geriatrics, Shenzhen 518020, China.
authors.forename: Lu-Jun
authors.initials: LJ
authors.lastname: Li
authors.forename: Peng
authors.initials: P
authors.lastname: Du
authors.forename: Ke-Huan
authors.initials: KH
authors.lastname: Sun
authors.forename: Mei-Qun
authors.initials: MQ
authors.lastname: Cao
authors.forename: Zheng-Zhi
authors.initials: ZZ
authors.lastname: Wu
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D018938:Caco-2 Cells; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006801:Humans; D030017:Ilex / Q000737:chemistry*; D020128:Inhibitory Concentration 50; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry*; D010946:Plants, Medicinal / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D010936:Plant Extracts