title: An integrated strategy for in vivo metabolite profiling using high-resolution mass spectrometry based data processing techniques.
abstract: An ongoing challenge of drug metabolite profiling is to detect and identify unknown or low-level metabolites in complex biological matrices. Here we present a generic strategy for metabolite detection using multiple accurate-mass-based data processing tools via the analysis of rat samples of two model drug candidates, AZD6280 and AZ12488024. First, the function of isotopic pattern recognition was proved to be highly effective in the detection of metabolites derived from [(14)C]-AZD6280 that possesses a distinct isotopic pattern. The metabolites revealed using this approach were in excellent qualitative correlation to those observed in radiochromatograms. Second, the effectiveness of accurate mass based untargeted data mining tools such as background subtraction, mass defect filtering, or a data mining package (MZmine) used for metabolomic analysis in detection of metabolites of [(14)C]-AZ12488024 in rat urine, feces, bile and plasma samples was examined and a total of 33 metabolites of AZ12488024 were detected. Among them, at least 16 metabolites were only detected by the aid of the data mining packages and not via radiochromatograms. New metabolic pathways such as S-oxidation and thiomethylation reactions occurring on the thiazole ring were proposed based on the processed data. The results of these experiments also demonstrated that accurate mass-based mass defect filtering (MDF) and data mining techniques used in metabolomics are complementary and can be valuable tools for delineating low-level metabolites in complex matrices. Furthermore, the application of distinct multiple data-mining algorithms in parallel, or in tandem, can be effective for rapidly profiling in vivo drug metabolites.
authors.affiliation: DMPK of Infection Innovative Medicine, AstraZeneca Pharmaceuticals, Waltham, MA 02451, USA. jian.guo@astrazeneca.com
authors.forename: Jian
authors.initials: J
authors.lastname: Guo
authors.forename: Minli
authors.initials: M
authors.lastname: Zhang
authors.forename: Charles S
authors.initials: CS
authors.lastname: Elmore
authors.forename: Karthick
authors.initials: K
authors.lastname: Vishwanathan
mesh_terms: D000818:Animals; D001646:Bile / Q000737:chemistry; D057225:Data Mining / Q000379:methods; D001330:Electronic Data Processing / Q000379:methods*; D005243:Feces / Q000737:chemistry; D006574:Heterocyclic Compounds, 2-Ring / Q000097:blood / Q000378:metabolism* / Q000652:urine; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D010880:Piperidines / Q000097:blood / Q000378:metabolism* / Q000652:urine; D011804:Quinolines / Q000097:blood / Q000378:metabolism* / Q000652:urine; D051381:Rats
chemical_list: C584464:4-((4-((2-hydroxyethyl)methylcarbamoyl)phenyl)quinolin-8-yl-methylene)-1-thiazol-4-ylmethylpiperidinium; C584463:4-amino-8-(2,5-dimethoxyphenyl)-N-propylcinnoline-3-carboxamide; D006574:Heterocyclic Compounds, 2-Ring; D010880:Piperidines; D011804:Quinolines