title: Chemiluminescence detector based on a single planar transparent digital microfluidic device.
abstract: We report on a compact and portable prototype of chemiluminescence detector based on a single planar single polar transparent electrowetting-on-dielectrics (EWOD) device. The coupling ground model was proposed to build the EWOD device, which could be driven under a single polar voltage. Such a design not only simplified the chip construction and control circuit, but also had the potential for the ball-like droplet to focus the fluorescence and enhance the detection sensitivity. Simulations and experiments both confirmed that the greater the contact angle, the stronger the detected optical signal, and thus the higher the sensitivity. The sensitivity of the prototype detector to H2O2 was 5.45 mV (mmol L(-1))(-1) and the detection limit was 0.01 mmol L(-1) when the contact angle of the EWOD surface was 120°. To further increase the sensitivity and decrease the detection limit, the contact angle of the EWOD device could be increased and the dark current of the photomultiplier decreased. The prototype shows potential applications as highly sensitive, cost effective and portable immuno-detectors, especially as a blood glucose monitor.
authors.affiliation: Department of Microelectronics, Fudan University, 220 Handan RD, Shanghai 200433, China.
authors.forename: Xiangyu
authors.initials: X
authors.lastname: Zeng
authors.forename: Kaidi
authors.initials: K
authors.lastname: Zhang
authors.forename: Jian
authors.initials: J
authors.lastname: Pan
authors.forename: Guoping
authors.initials: G
authors.lastname: Chen
authors.forename: Ai-Qun
authors.initials: AQ
authors.lastname: Liu
authors.forename: Shih-Kang
authors.initials: SK
authors.lastname: Fan
authors.forename: Jia
authors.initials: J
authors.lastname: Zhou
mesh_terms: D001786:Blood Glucose / Q000032:analysis; D055615:Electrical Equipment and Supplies; D058874:Electrowetting; D004867:Equipment Design; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D012815:Signal Processing, Computer-Assisted
chemical_list: D001786:Blood Glucose; D006861:Hydrogen Peroxide