title: Combination therapy of human adipose-derived stem cells and basic fibroblast growth factor hydrogel in muscle regeneration.
abstract: Skeletal muscle regeneration after sport injury is inconsistent, and complete healing without fibrosis is very important. In this study, we determined whether the combination therapy using human adipose-derived stem cells (h-ADSCs) and basic fibroblast growth factor (bFGF) incorporated into hydrogel could enhance muscle regeneration in a muscle laceration animal model. The h-ADSCs and/or bFGF hydrogels were applied to the lacerated gastrocnemius muscle. Fast twitch muscle contraction improved significantly and fibrosis decreased significantly in combined h-ADSC and bFGF-hydrogel group compared to other experimental groups. Skeletal muscle differentiation of h-ADSCs was determined by immunohistochemistry (PKH-26/MyHC co-staining) and Western blot. Our data suggested that combination therapy of h-ADSCs and bFGF hydrogel resulted in functional recovery, revascularization and reinnervation with minimal fibrosis in lacerated muscle. A combination of h-ADSCs and bFGF hydrogel can be used as a promising therapy for skeletal muscle regeneration.
authors.affiliation: Department of Physical and Rehabilitation Medicine, Sungkyunkwan University School of Medicine, Samsung Medical Center, Seoul, South Korea.
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Hwang
authors.forename: In Gul
authors.initials: IG
authors.lastname: Kim
authors.forename: Shuyu
authors.initials: S
authors.lastname: Piao
authors.forename: Ae Ryang
authors.initials: AR
authors.lastname: Jung
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Lee
authors.forename: Ki Dong
authors.initials: KD
authors.lastname: Park
authors.forename: Ji Youl
authors.initials: JY
authors.lastname: Lee
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D009119:Muscle Contraction / Q000187:drug effects; D018482:Muscle, Skeletal / Q000187:drug effects / Q000502:physiology*; D012038:Regeneration / Q000187:drug effects
chemical_list: D016222:Fibroblast Growth Factor 2; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate