title: Overexpression of carbonic anhydrase II and Ki-67 proteins in prognosis of gastrointestinal stromal tumors.
abstract: OBJECTIVE
  To investigate the expression and prognostic value of carbonic anhydrase II (CA II) and Ki-67 in gastrointestinal stromal tumors (GISTs).
  
  
  METHODS
  One hundred and thirteen GIST patients admitted to Chinese People's Liberation Army General Hospital from January 2004 to December 2010 were retrospectively followed up, and immunohistochemistry was used to detect CA II, Ki-67 and CD117 expression in tumor samples. The survival rates of the patients were analyzed using the Kaplan-Meier method. Log-rank test, χ² test and Cox proportional hazards model were used to determine the relationships between CA II, Ki-67 and CD117 expression and prognostic value in GISTs.
  
  
  RESULTS
  The survival rates at 1, 3 and 5 years were 90.0%, 82.0% and 72.0% in all patients. However, in patients with positive CA II or Ki-67, the survival rates were 92.0%, 83.0% and 77.0% or 83.0%, 66.6% and 53.0%, respectively. Compared with the negative groups, the survival rates in the positive groups were significantly lower (CA II log-rank P = 0.000; Ki-67 log-rank P = 0.004). Multivariate Cox analysis revealed that CA II, CD117 and Ki-67 were considerable immune factors in prognosis of GIST patients (CA II P = 0.043; CD117 P = 0.042; Ki-67 P = 0.007). Besides, tumor diameter, mitotic rate, tumor site, depth of invasion, complete resection, intraoperative rupture, and adjuvant therapy were important prognosis predictive factors. Our study indicated that CA II had strong expression in GISTs and the prognosis of GISTs with high CA II expression was better than that of GISTs with low or no expression, suggesting that CA II is both a diagnostic and prognostic biomarker for GIST.
  
  
  CONCLUSIONS
  CA II and Ki-67 are significant prognostic factors for GISTs. CA II associated with neovascular endothelia could serve as a potential target for cancer therapy.
authors.affiliation: Department of General Surgery, General Hospital of PLA, Beijing 100853, China.
authors.forename: Li-Cheng
authors.initials: LC
authors.lastname: Liu
authors.forename: Wen-Tong
authors.initials: WT
authors.lastname: Xu
authors.forename: Xin
authors.initials: X
authors.lastname: Wu
authors.forename: Po
authors.initials: P
authors.lastname: Zhao
authors.forename: Ya-Li
authors.initials: YL
authors.lastname: Lv
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
mesh_terms: D001706:Biopsy; D024402:Carbonic Anhydrase II / Q000032:analysis*; D016009:Chi-Square Distribution; D002681:China; D005260:Female; D046152:Gastrointestinal Stromal Tumors / Q000201:enzymology* / Q000401:mortality / Q000473:pathology / Q000628:therapy; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D019394:Ki-67 Antigen / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D008940:Mitotic Index; D015999:Multivariate Analysis; D009361:Neoplasm Invasiveness; D011379:Prognosis; D016016:Proportional Hazards Models; D019009:Proto-Oncogene Proteins c-kit / Q000032:analysis; D012189:Retrospective Studies; D012307:Risk Factors; D013997:Time Factors; D015854:Up-Regulation
chemical_list: D019394:Ki-67 Antigen; D019009:Proto-Oncogene Proteins c-kit; D024402:Carbonic Anhydrase II
keywords: CD117; Carbonic anhydrase; Gastrointestinal stromal tumors; Ki-67; Prognostic factor