title: Changes in CD34+ cell count in peripheral blood after whole blood donation.
abstract: OBJECTIVE
  We aimed to investigate the change in the number of stem cells and white cells in the early period following blood donation.
  
  
  METHODS
  22 male (71%) and 9 female (29%), 31 volunteers in total were included in the study. 450 ml of whole blood were collected from each of the volunteers for the donation. Complete blood counts were performed on the volunteers before and at 6 and 24h after the donation and CD34+ cell counts per ml of peripheral blood were measured by flow cytometry technique.
  
  
  RESULTS
  There was a statistically significant increase in the number of CD34+ cells in the peripheral blood at 6h following blood donation (p<0.001). At 24h, however, there was a statistically significant decrease in the number of CD34+ cells, compared to 6h (p<0.001). There was a statistically significant increase in the number of leukocytes in the peripheral blood at 6h following blood donation (p<0.001). At 24h, there was a decrease in the number of leukocytes, which was statistically significant compared to 6h (p<0.001). When the difference in CD34+ cell and leukocytes counts before blood donation and at 24h after blood donation were compared, the results were not statistically significant.
  
  
  CONCLUSIONS
  As the result of this study, a transient increase in the number of CD34+ cells in the peripheral blood after blood donation was demonstrated, with a decline in CD34+ cell counts back to levels prior to donation at 24h.
authors.affiliation: Erciyes University, Faculty of Medicine, Department of Hematology, Kayseri, Turkey. Electronic address: drcigdempala@gmail.com.
authors.forename: Cigdem
authors.initials: C
authors.lastname: Pala
authors.forename: Haluk
authors.initials: H
authors.lastname: Mumcuoglu
authors.forename: Fatih
authors.initials: F
authors.lastname: Kurnaz
authors.forename: Serdar
authors.initials: S
authors.lastname: Sivgin
authors.forename: Leylagul
authors.initials: L
authors.lastname: Kaynar
authors.forename: Muzaffer
authors.initials: M
authors.lastname: Keklik
authors.forename: Gulsah
authors.initials: G
authors.lastname: Akyol
authors.forename: Yavuz
authors.initials: Y
authors.lastname: Koker
authors.forename: Bulent
authors.initials: B
authors.lastname: Eser
mesh_terms: D000328:Adult; D018952:Antigens, CD34 / Q000097:blood*; D001782:Blood Donors; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D008297:Male; D013234:Stem Cells; D013997:Time Factors
chemical_list: D018952:Antigens, CD34
keywords: Blood donation; CD34; Peripheral stem cell