title: Peripherally applied synthetic peptide isoAsp7-Aβ(1-42) triggers cerebral β-amyloidosis.
abstract: Intracerebral and intraperitoneal inoculation with β-amyloid-rich brain extracts originating from patients with Alzheimer's disease as well as intracerebral injection of aggregates composed of synthetic Aβ can induce cerebral β-amyloidosis, and associated cognitive dysfunctions in susceptible animal hosts. We have found that repetitive intravenous administration of 100 μg of synthetic peptide corresponding to isoAsp7-containing Aβ(1-42), an abundant age-dependent Aβ isoform present both in the pathological brain and in synthetic Aβ preparations, robustly accelerates formation of classic dense-core congophilic amyloid plaques in the brain of β-amyloid precursor protein transgenic mice. Our findings indicate this peptide as an inductive agent of cerebral β-amyloidosis in vivo.
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov Street 32, 119991, Moscow, Russia. kozinsa@gmail.com
authors.forename: S A
authors.initials: SA
authors.lastname: Kozin
authors.forename: I B
authors.initials: IB
authors.lastname: Cheglakov
authors.forename: A A
authors.initials: AA
authors.lastname: Ovsepyan
authors.forename: G B
authors.initials: GB
authors.lastname: Telegin
authors.forename: P O
authors.initials: PO
authors.lastname: Tsvetkov
authors.forename: A V
authors.initials: AV
authors.lastname: Lisitsa
authors.forename: A A
authors.initials: AA
authors.lastname: Makarov
mesh_terms: D000544:Alzheimer Disease / Q000139:chemically induced / Q000473:pathology; D000595:Amino Acid Sequence; D016229:Amyloid beta-Peptides / Q000008:administration & dosage / Q000633:toxicity*; D000686:Amyloidosis / Q000139:chemically induced* / Q000473:pathology; D000818:Animals; D001921:Brain / Q000187:drug effects* / Q000473:pathology; D007275:Injections, Intravenous; D026581:Isoaspartic Acid / Q000008:administration & dosage / Q000235:genetics / Q000633:toxicity*; D008297:Male; D051379:Mice; D008809:Mice, Inbred C3H; D008822:Mice, Transgenic; D008969:Molecular Sequence Data; D010446:Peptide Fragments / Q000008:administration & dosage / Q000633:toxicity*; D058225:Plaque, Amyloid / Q000139:chemically induced* / Q000473:pathology
chemical_list: D016229:Amyloid beta-Peptides; D026581:Isoaspartic Acid; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42)