title: Histone deacetylase 3 implicated in the pathogenesis of children glioma by promoting glioma cell proliferation and migration.
abstract: Glioma is an aggressive cancer with high mortality, especially in children. It is known that histone modification plays an important role in the pathogenesis of various cancers. However, it is unknown whether histone deacetylase 3 (HDAC3) plays a role in the tumorigenesis of children gliomas. This study was aimed to explore the potential effects of HDAC3 in children gliomas. Expression of HDAC3 was measured in children glioma samples (n=70) and normal brain tissues (n=7) by real-time PCR and western blotting. Survival of the two groups was analyzed by the Kaplan-Meier method. The effects of HDAC3 in the pathogenesis of gliomas were analyzed by silencing the glioma cells U87-MG and U251. Data showed that HDAC3 was significantly elevated in children's gliomas, following the glioma grade, with almost no expression in normal brain tissues. Ectopic HDAC3 expression was correlated with poorer prognosis of children with glioma. In glioma cell lines, inhibition of HDAC3 using siRNA could suppress proliferation and sphere formation, induce G0/G1 arrest and apoptosis, and suppress the migration of glioma cells in comparison with controls. The higher level of HDAC3 expression was associated with more advanced tumor grades and shorter survival. HDAC3 participated in the pathogenesis of children gliomas by promoting glioma cell proliferation and migration.
authors.affiliation: Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhu
authors.forename: Hong
authors.initials: H
authors.lastname: Wan
authors.forename: Chaoqiang
authors.initials: C
authors.lastname: Xue
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.forename: Cheng
authors.initials: C
authors.lastname: Qian
authors.forename: Yuqi
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000818:Animals; D015153:Blotting, Western; D001932:Brain Neoplasms / Q000201:enzymology* / Q000401:mortality / Q000473:pathology; D002465:Cell Movement; D049109:Cell Proliferation; D002648:Child; D005260:Female; D005910:Glioma / Q000201:enzymology* / Q000401:mortality / Q000473:pathology; D006655:Histone Deacetylases / Q000378:metabolism*; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction; D046888:Tissue Array Analysis; D000099186:Histone Deacetylase 3
chemical_list: D006655:Histone Deacetylases; D000099186:Histone Deacetylase 3