title: Mapping the extracellular and membrane proteome associated with the vasculature and the stroma in the embryo.
abstract: In order to map the extracellular or membrane proteome associated with the vasculature and the stroma in an embryonic organism in vivo, we developed a biotinylation technique for chicken embryo and combined it with mass spectrometry and bioinformatic analysis. We also applied this procedure to implanted tumors growing on the chorioallantoic membrane or after the induction of granulation tissue. Membrane and extracellular matrix proteins were the most abundant components identified. Relative quantitative analysis revealed differential protein expression patterns in several tissues. Through a bioinformatic approach, we determined endothelial cell protein expression signatures, which allowed us to identify several proteins not yet reported to be associated with endothelial cells or the vasculature. This is the first study reported so far that applies in vivo biotinylation, in combination with robust label-free quantitative proteomics approaches and bioinformatic analysis, to an embryonic organism. It also provides the first description of the vascular and matrix proteome of the embryo that might constitute the starting point for further developments.
authors.affiliation: INSERM, U1029, 33405 Talence, France.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Soulet
authors.forename: Witold W
authors.initials: WW
authors.lastname: Kilarski
authors.forename: Florence
authors.initials: F
authors.lastname: Roux-Dalvai
authors.forename: John M J
authors.initials: JM
authors.lastname: Herbert
authors.forename: Izabela
authors.initials: I
authors.lastname: Sacewicz
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Mouton-Barbosa
authors.forename: Roy
authors.initials: R
authors.lastname: Bicknell
authors.forename: Patricia
authors.initials: P
authors.lastname: Lalor
authors.forename: Bernard
authors.initials: B
authors.lastname: Monsarrat
authors.forename: Andreas
authors.initials: A
authors.lastname: Bikfalvi
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000378:metabolism*; D019921:Biotinylation; D045744:Cell Line, Tumor; D002642:Chick Embryo / Q000378:metabolism*; D049033:Chorioallantoic Membrane / Q000293:injuries / Q000378:metabolism*; D042783:Endothelial Cells / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D006801:Humans; D007421:Intestine, Small / Q000196:embryology / Q000378:metabolism; D007668:Kidney / Q000196:embryology / Q000378:metabolism; D008099:Liver / Q000196:embryology / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D009369:Neoplasms / Q000378:metabolism; D020543:Proteome
chemical_list: D030161:Avian Proteins; D016326:Extracellular Matrix Proteins; D008565:Membrane Proteins; D020543:Proteome