title: Identification of biomarkers for endometriosis in eutopic endometrial cells from patients with endometriosis using a proteomics approach.
abstract: Endometriosis is a gynecological disease defined as the presence of endometrial tissue outside the uterine cavity, which is caused by various factors. Proteomic analysis of two sets of eutopic endometrial cells collected from the menstrual blood of females with (n=6; n=3) or without (n=6; n=3) endometriosis was performed to identify novel potential biomarkers for endometriosis. The data revealed that samples from endometriosis patients had stem cell characteristics, as they had higher mRNA expression levels of octamer-binding transcription factor 4 (Oct-4), C-X-C chemokine receptor type 4 (CXCR4), SRY-box containing gene 2 (SOX2) and mesenchymal-epithelial transition factor (MET) compared with that of the normal controls. Three proteins, collapsin response mediator protein 2 (CRMP2), ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCH-L1) and myosin regulatory light polypeptide 9 (MYL9), were simultaneously identified from the two sets of samples from females with or without endometriosis by two-dimensional electrophoresis (2-DE). A difference in CRMP2 expression was confirmed with western blotting. Taken together, the results suggest that CRMP2 plays a role in the pathogenesis of endometriosis.
authors.affiliation: Department of Animal Science and Technology, College of Animal Bioscience & Technology, Konkuk University, Seoul 143‑701, Republic of Korea.
authors.forename: Jin-Hee
authors.initials: JH
authors.lastname: Hwang
authors.forename: Jin-Ju
authors.initials: JJ
authors.lastname: Oh
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Yong-Cheng
authors.initials: YC
authors.lastname: Jin
authors.forename: Jae-Sung
authors.initials: JS
authors.lastname: Lee
authors.forename: Jong-Ryeol
authors.initials: JR
authors.lastname: Choi
authors.forename: Kyu-Sup
authors.initials: KS
authors.lastname: Lee
authors.forename: Jong-Kil
authors.initials: JK
authors.lastname: Joo
authors.forename: Hong-Gu
authors.initials: HG
authors.lastname: Lee
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics; D004715:Endometriosis / Q000235:genetics / Q000378:metabolism*; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000378:metabolism; D050814:Octamer Transcription Factor-3 / Q000235:genetics / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism
chemical_list: D015415:Biomarkers; D036341:Intercellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D050814:Octamer Transcription Factor-3; D012333:RNA, Messenger; D019718:Receptors, CXCR4; C095114:collapsin response mediator protein-2