title: Single-walled carbon nanotubes mediated neovascularity targeted antitumor drug delivery system.
abstract: OBJECTIVE
  The aim of this study was to prepare a new neovascularity targeting antitumor drug delivery system mediated by single-walled carbon nanotubes (SWNTs).
  
  
  METHODS
  In this study, antiangiogenesis agent 2-methoxyestradiol was loaded by SWNTs via π~π accumulation. The SWNTs were then linked with NGR (Asn-Gly-Arg) peptide, which could target tumor angiogenesis. This drug delivery system was characterized by transmission electron microscope, scanning electron microscopy, and atomic force microscope analysis. The suppression efficacy of tumor growth in cultured breast cancer cell line was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The in vivo antitumor activity was evaluated on the Sarcoma (S180) tumor-bearing mice model.
  
  
  RESULTS
  The characteristics of this drug delivery system showed that the particle of complex was 190 ± 4.3 nm in size distribution and 23.56 ± 2.03 mV in zeta potential. The inhibition ratio of this SWNTs drug delivery system at 24, 48, and 72 h was about 57.7%, 83.6%, and 88.2%. Compared with normal saline group, the relative tumor volumes in the 2ME, SWNTs-2ME, and NGR-SWNTs-2ME groups were decreased 1 week after administration.
  
  
  CONCLUSIONS
  This novel neovascularity targeting drug delivery system containing NGR-SWNTs-2ME may be beneficial to improve treatment efficacy and minimize side effects in future cancer therapy.
authors.affiliation: School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, China.
authors.forename: Chengqun
authors.initials: C
authors.lastname: Chen
authors.forename: Huijuan
authors.initials: H
authors.lastname: Zhang
authors.forename: Lin
authors.initials: L
authors.lastname: Hou
authors.forename: Jinjin
authors.initials: J
authors.lastname: Shi
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Chaofeng
authors.initials: C
authors.lastname: Zhang
authors.forename: Mingyue
authors.initials: M
authors.lastname: Zhang
authors.forename: Hongling
authors.initials: H
authors.lastname: Zhang
authors.forename: Xiufang
authors.initials: X
authors.lastname: Shi
authors.forename: Huixiang
authors.initials: H
authors.lastname: Li
authors.forename: Zhenzhong
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D000077584:2-Methoxyestradiol; D000818:Animals; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems; D004958:Estradiol / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D037742:Nanotubes, Carbon; D009369:Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000188:drug therapy; D009842:Oligopeptides / Q000008:administration & dosage* / Q000493:pharmacokinetics; D014018:Tissue Distribution; D050257:Tubulin Modulators / Q000008:administration & dosage* / Q000493:pharmacokinetics
chemical_list: C526428:NGR peptide; D037742:Nanotubes, Carbon; D009842:Oligopeptides; D050257:Tubulin Modulators; D004958:Estradiol; D000077584:2-Methoxyestradiol