title: An HPLC-HR-MS-MS method for identification of anticoagulant rodenticides in blood.
abstract: This paper presents a fully validated method for the qualitative identification of bromadiolone, brodifacoum, coumachlor, coumatetralyl, difenacoum and warfarin in whole blood specimens. Samples are protein precipitated with acetonitrile, processed via solid-phase extraction and analyzed by high-performance liquid chromatography with high resolution tandem mass spectrometric detection. Limits of detection were 10 ng/mL or better for all analytes.
authors.affiliation: Federal Bureau of Investigation Laboratory, Quantico, Virginia, USA. jason.schaff@ic.fbi.gov
authors.forename: Jason E
authors.initials: JE
authors.lastname: Schaff
authors.forename: Madeline A
authors.initials: MA
authors.lastname: Montgomery
mesh_terms: D015110:4-Hydroxycoumarins / Q000097:blood; D000097:Acetonitriles / Q000737:chemistry; D000925:Anticoagulants / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003374:Coumarins / Q000097:blood; D006801:Humans; D057230:Limit of Detection; D015203:Reproducibility of Results; D012378:Rodenticides / Q000097:blood*; D013048:Specimen Handling; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014859:Warfarin / Q000097:blood
chemical_list: D015110:4-Hydroxycoumarins; D000097:Acetonitriles; D000925:Anticoagulants; D003374:Coumarins; D012378:Rodenticides; D014859:Warfarin; C013418:bromfenacoum; C021636:bromadiolone; C004699:coumatetralyl; C012903:difenacoum; C412550:coumachlor; C032159:acetonitrile