title: Improvement of modified charcoal-cefoperazone-deoxycholate agar by addition of potassium clavulanate for detecting Campylobacter spp. in chicken carcass rinse.
abstract: The presence of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli (E. coli) in raw poultry is one of the most common factors that interfere with the isolation of Campylobacter by cefoperazone-based selective agar. The performance of modified charcoal-cefoperazone-deoxycholate agar (mCCDA) was improved by addition of an ESBL inhibitor, potassium clavulanate (0.5 mg/L). The ability of the supplemented medium (C-mCCDA) to detect Campylobacter species from chicken carcass rinse was compared with that of normal mCCDA. The isolation rate using C-mCCDA was significantly (p<0.05) higher compared with that using mCCDA (C-mCCDA, 67 out of 120; mCCDA, 38 out of 120). Furthermore, the selectivity of the C-mCCDA as assessed by comparing the number of contaminated plates (C-mCCDA, 44 out of 120; mCCDA, 110 out of 120) and growth index (C-mCCDA, 1.76; mCCDA, 2.79) of competing flora was also better (p<0.05) than that of mCCDA.
authors.affiliation: KU Center for Food Safety, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Jung-Whan
authors.initials: JW
authors.lastname: Chon
authors.forename: Hyunsook
authors.initials: H
authors.lastname: Kim
authors.forename: Hong-Seok
authors.initials: HS
authors.lastname: Kim
authors.forename: Kun-Ho
authors.initials: KH
authors.lastname: Seo
mesh_terms: D000362:Agar / Q000737:chemistry; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001431:Bacteriological Techniques / Q000379:methods*; D002167:Campylobacter / Q000187:drug effects / Q000254:growth & development / Q000302:isolation & purification; D002438:Cefoperazone / Q000494:pharmacology; D002606:Charcoal / Q000737:chemistry; D002645:Chickens; D019818:Clavulanic Acid / Q000494:pharmacology; D003470:Culture Media / Q000737:chemistry*; D003840:Deoxycholic Acid / Q000494:pharmacology; D005516:Food Microbiology / Q000379:methods*; D008460:Meat / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D003840:Deoxycholic Acid; D002606:Charcoal; D019818:Clavulanic Acid; D002438:Cefoperazone; D000362:Agar