title: Diagnosis of antigenic markers of acute toxoplasmosis by IgG avidity immunoblotting.
abstract: To perform IgG avidity immunoblotting assay for detection of acute toxoplasmosis, 100 serum samples were collected from Tehran, Iran. The presence of Toxoplasma-specific IgG and IgM antibodies were checked by commercial Trinity kit. Samples were categorized in acute and chronic phases of Toxoplasma gondii infection according to IgG avidity ELISA. IgG avidity immunoblotting was performed, and antigenic bands with molecular weights of 22, 25, 28, 30, 32, 42, 44, 49, 55, 60, 66, 69, 88, 106, 130 and 157 kDa were recognized as low avidity markers. The most prevalent antigen for low avidity was p22. It is concluded that IgG avidity immunoblotting could distinguish acute and chronic phases of T. gondii infection.
authors.affiliation: School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Siamak
authors.initials: S
authors.lastname: Ali-Heydari
authors.forename: Hossien
authors.initials: H
authors.lastname: Keshavarz
authors.forename: Saeedeh
authors.initials: S
authors.lastname: Shojaee
authors.forename: Mehdi
authors.initials: M
authors.lastname: Mohebali
mesh_terms: D000208:Acute Disease; D000818:Animals; D000915:Antibody Affinity / Q000276:immunology*; D000953:Antigens, Protozoan / Q000032:analysis* / Q000276:immunology; D015415:Biomarkers / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D015151:Immunoblotting / Q000379:methods*; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology*; D007075:Immunoglobulin M / Q000097:blood; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D014122:Toxoplasma / Q000276:immunology*; D014123:Toxoplasmosis / Q000175:diagnosis* / Q000276:immunology
chemical_list: D000953:Antigens, Protozoan; D015415:Biomarkers; D007106:Immune Sera; D007074:Immunoglobulin G; D007075:Immunoglobulin M