title: DDRGK1 regulates NF-κB activity by modulating IκBα stability.
abstract: NF-κB is a ubiquitously expressed transcription factor that regulates a large number of genes in response to diverse physiological and pathological stimuli. The regulation of the transcriptional activity of NF-κB is often dependent on its interaction with IκBα. Proteins that bind to IκBα are critical regulators of NF-κB activity. DDRGK1 is a member of the DDRGK domain-containing protein family with unknown function. In this study, we showed that the depletion of DDRGK1 inhibits cell proliferation and invasion. Microarray analysis indicated that the expression of NF-κB target genes showed the most significant decrease after depleting of DDRGK1, suggesting that DDRGK1 may play an important role in the NF-κB signaling pathway. We further demonstrated that DDRGK1 interacts with IκBα and regulates its stability, thereby regulates the NF-κB transcriptional activity. Our findings identify DDRGK1 as an important regulator of the NF-κB pathway.
authors.affiliation: Key Laboratory for Cell Proliferation and Regulation Biology of Ministry of Education, Institute of Cell Biology, College of Life Sciences, Beijing Normal University, Beijing, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Xi
authors.forename: Deqiang
authors.initials: D
authors.lastname: Ding
authors.forename: Junzhi
authors.initials: J
authors.lastname: Zhou
authors.forename: Miao
authors.initials: M
authors.lastname: Wang
authors.forename: Yu-Sheng
authors.initials: YS
authors.lastname: Cong
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D002460:Cell Line; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D016000:Cluster Analysis; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D020672:I-kappa B Proteins / Q000378:metabolism*; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000378:metabolism*; D011485:Protein Binding; D055550:Protein Stability; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D015533:Transcriptional Activation
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C000622456:DDRGK1 protein, human; D020672:I-kappa B Proteins; D016328:NF-kappa B; C000606204:NFKBIA protein, human; D011506:Proteins; D019938:Cyclin D1; D000072000:NF-KappaB Inhibitor alpha