title: Circulating Prostate Cells Found in Men with Benign Prostate Disease Are P504S Negative: Clinical Implications.
abstract: Introduction. Developments in immunological and quantitative real-time PCR-based analysis have enabled the detection, enumeration, and characterization of circulating tumor cells (CTCs). It is assumed that the detection of CTCs is associated with cancer, based on the finding that CTCs can be detected in all major cancer and not in healthy subjects or those with benign disease. Methods and Patients. Consecutive men, with suspicion of prostate cancer, had blood samples taken before prostate biopsy; mononuclear cells were obtained using differential gel centrifugation and CPCs detecting using anti-PSA immunocytochemistry. Positive samples underwent further classification with anti-P504S. Results. 329 men underwent prostate biopsy; of these men 83 underwent a second biopsy and 44 a third one. Of those with a biopsy negative for cancer, 19/226 (8.4%) had CPCs PSA (+) P504S (-) detected at first biopsy, 6/74 (8.1%) at second biopsy, and 5/33 (15.2%) at third biopsy. Men with cancer-positive biopsies did not have PSA (+) P504S (-) CPCs detected. These benign cells were associated with chronic prostatitis. Conclusions. Patients with chronic prostatitis may have circulating prostate cells detected in blood, which do not express the enzyme P504S and should be thought of as benign in nature.
authors.affiliation: Division of Medicine, Hospital Carabineros of Chile, Simón Bolívar 2200, Ñuñoa, 7770199 Santiago, Chile ; Instituto de BioOncología, Avenida Salvador 95, Oficina 95, Providencia, 7500710 Santiago, Chile ; Circulating Tumor Cell Unit, Faculty of Medicine Universidad Mayor, Renato Sánchez 4369, Las Condes, 7550224 Santiago, Chile.
authors.forename: Nigel P
authors.initials: NP
authors.lastname: Murray
authors.forename: Eduardo
authors.initials: E
authors.lastname: Reyes
authors.forename: Leonardo
authors.initials: L
authors.lastname: Badínez
authors.forename: Nelson
authors.initials: N
authors.lastname: Orellana
authors.forename: Cynthia
authors.initials: C
authors.lastname: Fuentealba
authors.forename: Ruben
authors.initials: R
authors.lastname: Olivares
authors.forename: José
authors.initials: J
authors.lastname: Porcell
authors.forename: Ricardo
authors.initials: R
authors.lastname: Dueñas