title: Analysis of defense signals in Arabidopsis thaliana leaves by ultra-performance liquid chromatography/tandem mass spectrometry: jasmonates, salicylic acid, abscisic acid.
abstract: Defense signaling compounds and phytohormones play an essential role in the regulation of plant responses to various environmental abiotic and biotic stresses. Among the most severe stresses are herbivory, pathogen infection, and drought stress. The major hormones involved in the regulation of these responses are 12-oxo-phytodienoic acid (OPDA), the pro-hormone jasmonic acid (JA) and its biologically active isoleucine conjugate (JA-Ile), salicylic acid (SA), and abscisic acid (ABA). These signaling compounds are present and biologically active at very low concentrations from ng/g to μg/g dry weight. Accurate and sensitive quantification of these signals has made a significant contribution to the understanding of plant stress responses. Ultra-performance liquid chromatography (UPLC) coupled with a tandem quadrupole mass spectrometer (MS/MS) has become an essential technique for the analysis and quantification of these compounds.
authors.affiliation: Pharmaceutical Biology, Julius-von-Sachs-Institute for Biosciences, University of Wuerzburg, Wuerzburg, Germany.
authors.forename: Nadja
authors.initials: N
authors.lastname: Stingl
authors.forename: Markus
authors.initials: M
authors.lastname: Krischke
authors.forename: Agnes
authors.initials: A
authors.lastname: Fekete
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Mueller
mesh_terms: D000040:Abscisic Acid / Q000032:analysis*; D017360:Arabidopsis / Q000276:immunology*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003517:Cyclopentanes / Q000032:analysis*; D054883:Oxylipins / Q000032:analysis*; D018515:Plant Leaves / Q000737:chemistry*; D012015:Reference Standards; D020156:Salicylic Acid / Q000032:analysis*; D015398:Signal Transduction / Q000276:immunology; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D003517:Cyclopentanes; D054883:Oxylipins; C011006:jasmonic acid; D000040:Abscisic Acid; D020156:Salicylic Acid