title: [Rapid recognition and identification of chemical constituents in Qinghuo Zhimai tablets by SPE-HPLC-ESI-MS(n)].
abstract: OBJECTIVE
  To establish a method for analyzing solid phase extraction-high performance liquid chromatography-electrospray ionization tandem mass spectrometry (SPE-HPLC-ESI-MS(n)), in order to recognize and identify the main chemical constituents in Qinghuo Zhimai tablets.
  
  
  METHODS
  The possible structures of the compounds were determined according to the structure information of compounds observed from molecular ion peaks and fragment ions in HPLC-ESI-MS(n) negative ion mode and by comparing with literature data or control samples.
  
  
  RESULTS
  Through the comparative analysis on Qinghuo Zhimai Tablets and components of its formula, 39 chemical constituents were identified, including 7 caffeoylquinic acids, 7 iridoids, 6 diterpenoid lactones, 5 homoisoflavonoids, 13 steroidal saponins and 1 flavone glycoside.
  
  
  CONCLUSIONS
  This study provides a simple and rapid method for identifying chemical components in Qinghuo Zhimai tablets.
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. zhangjiayu0615@163.com
authors.forename: Jia-Yu
authors.initials: JY
authors.lastname: Zhang
authors.forename: Yan-Jiang
authors.initials: YJ
authors.lastname: Qiao
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Gao
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhang
authors.forename: Qun
authors.initials: Q
authors.lastname: Ma
authors.forename: Jian-Qiu
authors.initials: JQ
authors.lastname: Lu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D047309:Flavones / Q000737:chemistry / Q000302:isolation & purification; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification; D039823:Iridoids / Q000737:chemistry / Q000302:isolation & purification; D007783:Lactones / Q000737:chemistry / Q000302:isolation & purification; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure; D011801:Quinic Acid / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification; D052616:Solid Phase Extraction / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013607:Tablets; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; D047309:Flavones; D006027:Glycosides; D039823:Iridoids; D007783:Lactones; D012503:Saponins; D013607:Tablets; C472707:caffeoylquinic acid; D011801:Quinic Acid; C043562:flavone