title: Kainic acid induces expression of caveolin-1 in activated microglia in rat brain.
abstract: Caveolin-1, a major constituent of caveolae, has been implicated in endocytosis, signal transduction and cholesterol transport in a wide variety of cells. In the present study, the expression of caveolin-1 was examined by immunohistochemistry in rat brain with or without systemic injection of kainic acid (KA). Caveolin-1 immunoreactivity was observed in capillary walls in brains of control rats. From one to seven days after KA injection, caveolin-1 immunoreactivity appeared in activated microglia in the cerebral cortex, hippocampus and other brain regions. The strongest immunoreactivity of microglia was seen after 3 days after KA administration. The expression of caveolin-1 was confirmed by RT-PCR and Western blot analysis, respectively. The induction of caveolin-1 expression in microglia activated in response to kainic acid administration suggests its possible role in a modulation of inflammation.
authors.affiliation: Molecular Neuroscience Research Center, Shiga University of Medical Science, Otsu, Japan.
authors.forename: Shigeko
authors.initials: S
authors.lastname: Takeuchi
authors.forename: Wakoto
authors.initials: W
authors.lastname: Matsuda
authors.forename: Ikuo
authors.initials: I
authors.lastname: Tooyama
authors.forename: Osamu
authors.initials: O
authors.lastname: Yasuhara
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D001921:Brain / Q000166:cytology*; D051242:Caveolin 1 / Q000235:genetics / Q000378:metabolism*; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D007608:Kainic Acid / Q000494:pharmacology*; D008297:Male; D017628:Microglia / Q000187:drug effects / Q000378:metabolism*; D018613:Microscopy, Confocal; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D015415:Biomarkers; D051242:Caveolin 1; D007608:Kainic Acid