title: [Separation and identification of endophytic fungi from desert plant Cynanchum komarovii].
abstract: OBJECTIVE
  The research aimed to investigate the entophytic fungal community of Cynanchum Komarrovii, including the biodiversity in different organs and the correlations with ecological environment. Endophytic fungi with patent bioactivity were also rapidly screened.
  
  
  METHODS
  PDA medium was used to isolate and purify the endophytic fungi from C. komarovii living in Shaanxi and Ningxia district, respectively. The strains were identified based on the morphological characteristics of the fungi and similarity of 5.8S gene and internal transcribed spacer (ITS) sequence. Pyriculaia oryzae model was applied to preliminarily screen the active fungi.
  
  
  RESULTS
  Ninety-four strains of endophytic fungi were isolated and identified to 9 species, 13 genera, 9 families and 6 orders, among them, 47 strains were from the plants living in Ningxia. And then, 5 of them were isolated from roots, 14 from branches, and 28 from leaves. They were identified belonging to 8 species, 9 genera, 5 families and 4 orders. Additionally, 47 strains were from the plants living in Shaanxi. 16 were isolated from the roots, 18 from branches, 13 from leaves. They were identified belonging to 5 species, 8 genera, 6 families and 4 orders. By preliminary screening, 18 strains of endophytes completely inhibited the germination of conidium, which showed a potential bioactivity for these fungi. Both N4 and S17 strains had stronger growth inhibition effect.
  
  
  CONCLUSIONS
  Endophytic fungi from desert plant C. komarovii have the feature of diversity. Different geographical environment and type of organizations lead to the significant difference on the quantity and the species composition. Most of fungi in Ningxia C. komarovii distribute in leaves. However, most of those in Shaanxi C. komarovii distribute in stems and leaves. It also indicated that endophytes from C. komarovii had a strong antifungal activity.
authors.affiliation: School of Pharmacy, Ningxia Medical University, Ningxia Research Center of Modern Hui Medicine Engineering and Techology, Yinchuan 750004, China.
authors.forename: Hai-Jing
authors.initials: HJ
authors.lastname: Duan
authors.forename: Ting
authors.initials: T
authors.lastname: Han
authors.forename: Xiu-Li
authors.initials: XL
authors.lastname: Wu
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Lu-Ping
authors.initials: LP
authors.lastname: Qin
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology; D044822:Biodiversity; D002681:China; D017077:Culture Media, Conditioned / Q000494:pharmacology; D031105:Cynanchum / Q000382:microbiology*; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D003889:Desert Climate; D060026:Endophytes / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D005658:Fungi / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D014644:Genetic Variation; D020082:Magnaporthe / Q000187:drug effects / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D010802:Phylogeny; D018515:Plant Leaves / Q000382:microbiology; D018517:Plant Roots / Q000382:microbiology; D018547:Plant Stems / Q000382:microbiology; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D013045:Species Specificity
chemical_list: D000935:Antifungal Agents; D017077:Culture Media, Conditioned; D021903:DNA, Ribosomal Spacer; D012340:RNA, Ribosomal, 5.8S