title: Gamma radiation mediated green synthesis of gold nanoparticles using fermented soybean-garlic aqueous extract and their antimicrobial activity.
abstract: Aspergillus oryzae was used to enhance the mobilization of antioxidants of soybean matrix along with garlic as a co-substrate by modulating polyphenolic substances during solid-state fermentation. Mobilized polyphenols were used as a green tool for synthesis and stabilization of gold nanoparticles (AuNPs). The radiation-induced AuNPs synthesis is a simple, clean and inexpensive process which involves radiolysis of aqueous solution that provides an efficient method to reduce metal ions. Gamma irradiated aqueous extract of fermented soybean and garlic was used for rapid preparation of AuNPs combining both effects of radiolytic reactions by radiation and stabilization by bioactive components of fermented extract. The synthesized AuNPs were confirmed by UV-Visible spectrophotometry, dynamic light scattering (DLS), Fourier Transform infra red (FT-IR) spectrophotometry, and transmission electron microscope (TEM) analysis which revealed morphology of spherical AuNPs with size ranging from 7-12 nm. The synthesized AuNPs exhibited antimicrobial activity against both Gram positive and Gram negative bacteria, as measured by well diffusion assay.
authors.affiliation: Drug Radiation Research Department, National Center for Radiation Research & Technology (NCRRT), Nasr City, P.O. Box 29, Cairo, Egypt.
authors.forename: Ahmed Ibrahim
authors.initials: AI
authors.lastname: El-Batal
authors.forename: Abd-Algawad M
authors.initials: AA
authors.lastname: Hashem
authors.forename: Noha M
authors.initials: NM
authors.lastname: Abdelbaky
keywords: Antimicrobial activity; Gamma radiation; Garlic; Gold nanoparticles; Solid state fermentation; Soybean