title: Dyella kyungheensis sp. nov., isolated from soil of a cornus fruit field.
abstract: A Gram-reaction-negative, aerobic, motile by one polar flagellum, yellow-pigmented, rod-shaped bacterium, designated strain THG-B117(T), was isolated from soil of a cornus fruit field of Hoengseong province in South Korea and its taxonomic position was investigated by a polyphasic study. Strain THG-B117(T) grew well at 25-30 °C and at pH 6.0-8.0 in the absence of NaCl on nutrient agar. On the basis of 16S rRNA gene sequence similarity, strain THG-B117(T) was shown to belong to the family Xanthomonadaceae and be related to Dyella japonica XD53(T) (98.7 % similarity), Dyella terrae JS14-6(T) (98.0 %), Dyella koreensis BB4(T) (96.9 %), Dyella soli JS12-10(T) (96.9 %) and Dyella thiooxydans ATSB10(T) (96.7 %). DNA-DNA hybridization experiments showed that DNA relatedness between strain THG-B117(T) and its phylogenetically closest neighbours was below 45.1 %. The G+C content of the genomic DNA of strain THG-B117(T) was 64.8 mol%. The major polar lipids were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, unidentified aminolipids, unidentified aminophospholipids and unidentified phospholipids. Phenotypic and chemotaxonomic data (major ubiquinone was Q-8, and major fatty acids were iso-C15 : 0, iso-C16 : 0, iso-C17 : 0 and iso-C17 : 1ω9c) supported the affiliation of strain THG-B117(T) with the genus Dyella. The results of physiological and biochemical tests suggested that strain THG-B117(T) was different genotypically and phenotypically from recognized species of the genus Dyella, and represents a novel species of this genus. The name Dyella kyungheensis sp. nov. is proposed, with the type strain THG-B117(T) ( = KACC 16981(T) = JCM 18747(T)).
authors.affiliation: Department of Oriental Medicinal Material & Processing, College of Life science, Kyung Hee University, 1 Seocheon, Kihung, Yongin, Kyunggi 446-701, Republic of Korea.
authors.forename: Heung-Min
authors.initials: HM
authors.lastname: Son
authors.affiliation: Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Republic of Korea.
authors.forename: Jung-Eun
authors.initials: JE
authors.lastname: Yang
authors.affiliation: Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Republic of Korea.
authors.forename: Eun-Ji
authors.initials: EJ
authors.lastname: Yi
authors.affiliation: Department of Oriental Medicinal Material & Processing, College of Life science, Kyung Hee University, 1 Seocheon, Kihung, Yongin, Kyunggi 446-701, Republic of Korea.
authors.forename: YongJin
authors.initials: Y
authors.lastname: Park
authors.affiliation: Department of Oriental Medicinal Material & Processing, College of Life science, Kyung Hee University, 1 Seocheon, Kihung, Yongin, Kyunggi 446-701, Republic of Korea.
authors.forename: Kyung-Hwa
authors.initials: KH
authors.lastname: Won
authors.affiliation: Department of Oriental Medicinal Material & Processing, College of Life science, Kyung Hee University, 1 Seocheon, Kihung, Yongin, Kyunggi 446-701, Republic of Korea.
authors.forename: Ju-Han
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Oriental Medicinal Material & Processing, College of Life science, Kyung Hee University, 1 Seocheon, Kihung, Yongin, Kyunggi 446-701, Republic of Korea.
authors.forename: Chang-Kyun
authors.initials: CK
authors.lastname: Han
authors.affiliation: Department of Marine Biotechnology, Anyang University, Incheon 417-833, Republic of Korea.
authors.forename: MooChang
authors.initials: M
authors.lastname: Kook
authors.affiliation: Department of Oriental Medicinal Material & Processing, College of Life science, Kyung Hee University, 1 Seocheon, Kihung, Yongin, Kyunggi 446-701, Republic of Korea.
authors.forename: Tae-Hoo
authors.initials: TH
authors.lastname: Yi
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D029768:Cornus; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D044166:Xanthomonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S