title: Low-intensity pulsed ultrasound induced enhanced adipogenesis of adipose-derived stem cells.
abstract: OBJECTIVE
  The aim of this study was to investigate effects of low-intensity pulsed ultrasound (LIPUS) on differentiation of adipose-derived stem cells (ASCs), in vitro.
  
  
  METHODS
  Murine ASCs were treated with LIPUS for either three or five days, immediately after adipogenic induction, or delayed for 2 days. Expression of adipogenic genes PPAR-γ1, and APN, was examined by real-time PCR. Immunofluorescence (IF) staining was performed to test for PPAR-γ at the protein level.
  
  
  RESULTS
  Our data revealed that specific patterns of LIPUS up-regulated levels of both PPAR-γ1 and APN mRNA, and PPAR-γ protein.
  
  
  CONCLUSIONS
  In culture medium containing adipogenic reagents, LIPUS enhanced ASC adipogenesis.
authors.affiliation: State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, China.
authors.forename: N
authors.initials: N
authors.lastname: Fu
authors.forename: X
authors.initials: X
authors.lastname: Yang
authors.forename: K
authors.initials: K
authors.lastname: Ba
authors.forename: Y
authors.initials: Y
authors.lastname: Fu
authors.forename: X
authors.initials: X
authors.lastname: Wei
authors.forename: Y
authors.initials: Y
authors.lastname: Yue
authors.forename: G
authors.initials: G
authors.lastname: Li
authors.forename: Y
authors.initials: Y
authors.lastname: Yao
authors.forename: J
authors.initials: J
authors.lastname: Chen
authors.forename: X
authors.initials: X
authors.lastname: Cai
authors.forename: C
authors.initials: C
authors.lastname: Liang
authors.forename: Y
authors.initials: Y
authors.lastname: Ge
authors.forename: Y
authors.initials: Y
authors.lastname: Lin
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000000981:diagnostic imaging* / Q000378:metabolism; D050156:Adipogenesis; D000273:Adipose Tissue / Q000166:cytology / Q000000981:diagnostic imaging* / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D059630:Mesenchymal Stem Cells / Q000000981:diagnostic imaging*; D051379:Mice; D047495:PPAR gamma / Q000096:biosynthesis / Q000378:metabolism; D014463:Ultrasonography
chemical_list: D047495:PPAR gamma