title: Intestinal caveolin-1 is important for dietary fatty acid absorption.
abstract: How dietary fatty acids are absorbed into the enterocyte and transported to the ER is not established. We tested the possibility that caveolin-1 containing lipid rafts and endocytic vesicles were involved. Apical brush border membranes took up 15% of albumin bound (3)H-oleate whereas brush border membranes from caveolin-1 KO mice took up only 1%. In brush border membranes, the (3)H-oleate was in the detergent resistant fraction of an OptiPrep gradient. On OptiPrep gradients of intestinal cytosol, we also found the (3)H-oleate in the detergent resistant fraction, separate from OptiPrep gradients spiked with (3)H-oleate or (3)H-triacylglycerol. Caveolin-1 immuno-depletion of cytosol removed 91% of absorbed (3)H-oleate whereas immuno-depletion using IgG, or anti-caveolin-2 or -3 or anti-clathrin antibodies removed 20%. Electron microscopy showed the presence of caveolin-1 containing vesicles in WT mouse cytosol that were 4 fold increased by feeding intestinal sacs 1mM oleate. No vesicles were seen in caveolin-1 KO mouse cytosol. Caveolin-1 KO mice gained less weight on a 23% fat diet and had increased fat in their stool compared to WT mice. We conclude that dietary fatty acids are absorbed by caveolae in enterocyte brush border membranes, are endocytosed, and transported in cytosol in caveolin-1 containing endocytic vesicles.
authors.affiliation: Department of Medicine, The University of Tennessee Health Science Center, TN, USA.
authors.forename: Shahzad
authors.initials: S
authors.lastname: Siddiqi
authors.forename: Atur
authors.initials: A
authors.lastname: Sheth
authors.forename: Feenalie
authors.initials: F
authors.lastname: Patel
authors.forename: Matthew
authors.initials: M
authors.lastname: Barnes
authors.forename: Charles M
authors.initials: CM
authors.lastname: Mansbach
mesh_terms: D000818:Animals; D051242:Caveolin 1 / Q000235:genetics / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D004041:Dietary Fats / Q000378:metabolism* / Q000494:pharmacology; D004705:Endocytosis / Q000502:physiology*; D020895:Enterocytes / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007408:Intestinal Absorption / Q000502:physiology*; D021962:Membrane Microdomains / Q000235:genetics / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D008871:Microvilli / Q000235:genetics / Q000378:metabolism; D019301:Oleic Acid / Q000378:metabolism* / Q000494:pharmacology; D051381:Rats
chemical_list: C495537:Cav1 protein, rat; D051242:Caveolin 1; D004041:Dietary Fats; D019301:Oleic Acid