title: Antigenotoxic potential of aqueous extracts from the chanterelle mushroom, Cantharellus cibarius (higher Basidiomycetes), on human mononuclear cell cultures.
abstract: Cantharellus cibarius is one of the most important wild, edible, and ectomycorrhizal mushrooms growing at La Malinche National Park, Tlaxcala, Mexico; therefore, the assessment of its biological properties is of great interest to know its potential as an alternative treatment to chemopreventive strategies when it is consumed as part of a diet. Comet assay was used to evaluate the antigenotoxic properties of several concentrations of aqueous extracts (0.0125, 0.025, 0.05, 0.1, and 0.2% w/v) prepared at room temperature (22 ± 2°C). As a test system we used human mononuclear cells exposed to methyl methanesulphonate (MMS) in vitro according to 3 different protocols: previous, simultaneous, and posterior. Previous (0.0125%) and simultaneous (0.1%) treatments resulted in the highest inhibitory efficiency. In the former, the cells assessed showed a tail length of 94.9 ± 64 µm; in the latter, the tails measured 106.2 ± 40 µm. Resulting percentages of reduction in damage were 236% and 196.1%, respectively. We did not obtain a dose-dependent response. The mean tail length for each protocol (previous, 133.1 ± 80 µm; simultaneous, 127.8 ± 57 µm; posterior, 146.3 ± 74 µm) was statistically significant with regard to the positive control (MMS).
authors.affiliation: Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias, Centro Nacional de Investigacion Disciplinaria en Conservacion y Mejoramiento de Ecosistemas Forestales, Coyoacan, Mexico. clyame@hotmail.com
authors.forename: Claudia
authors.initials: C
authors.lastname: Mendez-Espinoza
authors.forename: Edelmira
authors.initials: E
authors.lastname: Garcia-Nieto
authors.forename: Adriana Montoya
authors.initials: AM
authors.lastname: Esquivel
authors.forename: Monica Montiel
authors.initials: MM
authors.lastname: Gonzalez
authors.forename: Efrain Velasco
authors.initials: EV
authors.lastname: Bautista
authors.forename: Carmen Calderon
authors.initials: CC
authors.lastname: Ezquerro
authors.forename: Libertad Juarez
authors.initials: LJ
authors.lastname: Santacruz
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D005591:Chemical Fractionation; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects*; D014867:Water
chemical_list: D014867:Water