title: Promoter regions of potato vacuolar invertase gene in response to sugars and hormones.
abstract: Potato vacuolar acid invertase (StvacINV1) (β-fructofuranosidase; EC 3.2.1.26) has been confirmed to play an important role in cold-induced sweetening of potato tubers. However, the transcriptional regulation mechanisms of StvacINV1 are largely unknown. In this study, the 5'-flanking sequence of StvacINV1 was cloned and the cis-acting elements were predicted. Histochemical assay showed that the StvacINV1 promoter governed β-glucuronidase (GUS) expression in potato leaves, stems, roots and tubers. Quantitative analysis of GUS expression suggested that the activity of StvacINV1 promoter was suppressed by sucrose, glucose, fructose, and cold, while enhanced by indole-3-acetic acid (IAA), and gibberellic acid (GA3). Further deletion analysis clarified that the promoter regions from -118 to -551, -551 to -1021, and -1021 to -1521 were required for responding to sucrose/glucose, GA3, and IAA, respectively. These findings provide essential information regarding transcriptional regulation mechanisms of StvacINV1.
authors.affiliation: National Centre for Vegetable Improvement Central China, Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
authors.forename: Yongbin
authors.initials: Y
authors.lastname: Ou
authors.forename: Botao
authors.initials: B
authors.lastname: Song
authors.forename: Xun
authors.initials: X
authors.lastname: Liu
authors.forename: Conghua
authors.initials: C
authors.lastname: Xie
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lin
authors.forename: Huiling
authors.initials: H
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
mesh_terms: D018506:Gene Expression Regulation, Plant / Q000235:genetics; D005838:Genotype; D005875:Gibberellins / Q000494:pharmacology*; D005947:Glucose / Q000494:pharmacology*; D007210:Indoleacetic Acids / Q000494:pharmacology*; D018515:Plant Leaves / Q000187:drug effects / Q000201:enzymology; D018517:Plant Roots / Q000187:drug effects / Q000201:enzymology; D018547:Plant Stems / Q000187:drug effects / Q000201:enzymology; D011401:Promoter Regions, Genetic / Q000187:drug effects / Q000235:genetics*; D011198:Solanum tuberosum / Q000187:drug effects / Q000201:enzymology*; D013395:Sucrose / Q000494:pharmacology*; D014617:Vacuoles / Q000201:enzymology*; D043324:beta-Fructofuranosidase / Q000235:genetics*
chemical_list: D005875:Gibberellins; D007210:Indoleacetic Acids; D013395:Sucrose; C030737:indoleacetic acid; C007842:gibberellic acid; D043324:beta-Fructofuranosidase; D005947:Glucose