title: Serum calprotectin levels correlate with biochemical and histological markers of disease activity in TNBS colitis.
abstract: OBJECTIVE
  Serum calprotectin is elevated in patients with inflammatory bowel disease (IBD). Whether it correlates other markers of disease activity is unknown. The aim of this study was to correlate serum calprotectin with biochemical and histological measures of intestinal inflammation.
  
  
  METHODS
  TNBS colitis was induced in wistar rats, and serial blood samples were collected at 0, 3, and 12 days. Animals were subsequently sacrificed for pathological evaluation at day 12. Serum calprotectin and cytokines were measured by ELISA. Pathologic changes were classified at the macroscopic and microscopic levels.
  
  
  RESULTS
  TNBS colitis induced elevated serum calprotectin, TNF and IL-6 within 24 h. Levels of serum calprotectin remained elevated in parallel to persistence of loose stool and weight loss to day 12. Serum calprotectin levels correlated with serum levels of TNF-α and IL6 (p < 0.001), but not CRP. Animals with liquid stool had significantly higher levels of serum calprotectin than control animals. There was a correlation between macroscopic colitis scores, and levels of serum calprotectin.
  
  
  CONCLUSIONS
  Serum calprotectin levels correlate with biochemical and histological markers of inflammation in TNBS colitis. This biomarker may have potential for diagnostic use in patients with IBD.
authors.affiliation: Gastroenterology Department, Federal University of São Paulo, UNIFESP/EPM, Brazil. didia_cury@uol.com.br
authors.forename: Didia Bismara
authors.initials: DB
authors.lastname: Cury
authors.forename: Sender Jankiel
authors.initials: SJ
authors.lastname: Mizsputen
authors.forename: Clara
authors.initials: C
authors.lastname: Versolato
authors.forename: Luciana Odashiro
authors.initials: LO
authors.lastname: Miiji
authors.forename: Edson
authors.initials: E
authors.lastname: Pereira
authors.forename: Maria Aparecida
authors.initials: MA
authors.lastname: Delboni
authors.forename: Nestor
authors.initials: N
authors.lastname: Schor
authors.forename: Alan C
authors.initials: AC
authors.lastname: Moss
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D003092:Colitis / Q000097:blood* / Q000139:chemically induced / Q000473:pathology; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000737:chemistry; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000097:blood / Q000175:diagnosis; D015850:Interleukin-6 / Q000097:blood*; D039841:Leukocyte L1 Antigen Complex / Q000097:blood*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014302:Trinitrobenzenesulfonic Acid; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*; D015431:Weight Loss
chemical_list: D015415:Biomarkers; D015850:Interleukin-6; D039841:Leukocyte L1 Antigen Complex; D014409:Tumor Necrosis Factor-alpha; D014302:Trinitrobenzenesulfonic Acid