title: Structure and non-structure of centrosomal proteins.
abstract: Here we perform a large-scale study of the structural properties and the expression of proteins that constitute the human Centrosome. Centrosomal proteins tend to be larger than generic human proteins (control set), since their genes contain in average more exons (20.3 versus 14.6). They are rich in predicted disordered regions, which cover 57% of their length, compared to 39% in the general human proteome. They also contain several regions that are dually predicted to be disordered and coiled-coil at the same time: 55 proteins (15%) contain disordered and coiled-coil fragments that cover more than 20% of their length. Helices prevail over strands in regions homologous to known structures (47% predicted helical residues against 17% predicted as strands), and even more in the whole centrosomal proteome (52% against 7%), while for control human proteins 34.5% of the residues are predicted as helical and 12.8% are predicted as strands. This difference is mainly due to residues predicted as disordered and helical (30% in centrosomal and 9.4% in control proteins), which may correspond to alpha-helix forming molecular recognition features (α-MoRFs). We performed expression assays for 120 full-length centrosomal proteins and 72 domain constructs that we have predicted to be globular. These full-length proteins are often insoluble: Only 39 out of 120 expressed proteins (32%) and 19 out of 72 domains (26%) were soluble. We built or retrieved structural models for 277 out of 361 human proteins whose centrosomal localization has been experimentally verified. We could not find any suitable structural template with more than 20% sequence identity for 84 centrosomal proteins (23%), for which around 74% of the residues are predicted to be disordered or coiled-coils. The three-dimensional models that we built are available at http://ub.cbm.uam.es/centrosome/models/index.php.
authors.affiliation: Centro de Biología Molecular Severo Ochoa (CBMSO), CSIC-UAM, Madrid, Spain.
authors.forename: Helena G
authors.initials: HG
authors.lastname: Dos Santos
authors.forename: David
authors.initials: D
authors.lastname: Abia
authors.forename: Robert
authors.initials: R
authors.lastname: Janowski
authors.forename: Gulnahar
authors.initials: G
authors.lastname: Mortuza
authors.forename: Michela G
authors.initials: MG
authors.lastname: Bertero
authors.forename: Maïlys
authors.initials: M
authors.lastname: Boutin
authors.forename: Nayibe
authors.initials: N
authors.lastname: Guarín
authors.forename: Raúl
authors.initials: R
authors.lastname: Méndez-Giraldez
authors.forename: Alfonso
authors.initials: A
authors.lastname: Nuñez
authors.forename: Juan G
authors.initials: JG
authors.lastname: Pedrero
authors.forename: Pilar
authors.initials: P
authors.lastname: Redondo
authors.forename: María
authors.initials: M
authors.lastname: Sanz
authors.forename: Silvia
authors.initials: S
authors.lastname: Speroni
authors.forename: Florian
authors.initials: F
authors.lastname: Teichert
authors.forename: Marta
authors.initials: M
authors.lastname: Bruix
authors.forename: José M
authors.initials: JM
authors.lastname: Carazo
authors.forename: Cayetano
authors.initials: C
authors.lastname: Gonzalez
authors.forename: José
authors.initials: J
authors.lastname: Reina
authors.forename: José M
authors.initials: JM
authors.lastname: Valpuesta
authors.forename: Isabelle
authors.initials: I
authors.lastname: Vernos
authors.forename: Juan C
authors.initials: JC
authors.lastname: Zabala
authors.forename: Guillermo
authors.initials: G
authors.lastname: Montoya
authors.forename: Miquel
authors.initials: M
authors.lastname: Coll
authors.forename: Ugo
authors.initials: U
authors.lastname: Bastolla
authors.forename: Luis
authors.initials: L
authors.lastname: Serrano
mesh_terms: D000595:Amino Acid Sequence; D018385:Centrosome / Q000378:metabolism*; D030562:Databases, Protein; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D011485:Protein Binding; D017510:Protein Folding; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D020033:Protein Isoforms; D011506:Proteins; D020543:Proteome