title: The effect of calorie restriction on acute ethanol-induced oxidative and nitrosative liver injury in rats.
abstract: The aim of our study was to examine the effect of calorie restriction (CR) on oxidative and nitrosative liver injury in rats, induced by acute ethanol intoxication. Male Wistar rats were divided into groups: (1) control; (2) calorie-restricted groups with intake of 60-70% (CR60-70) and 40-50% of daily energy needs (CR40-50); (3) ethanol-treated group (E); (4) calorie-restricted, ethanol-treated groups (E+CR60-70 and E+CR40-50). Ethanol was administered in 5 doses of 2g/kg every 12h, and duration of CR was 5 weeks before ethanol treatment. Malondialdehyde and nitrite and nitrate level were significantly lower in E+CR60-70 and higher in E+CR40-50 vs. E group. Liver reduced glutathione content and activity of both superoxide dismutase izoenzymes were significantly higher in E+CR60-70 and lower in E+CR40-50 vs. E group. Oxidative stress may be a potential mechanism of hormetic effects of CR on acute ethanol-induced liver injury.
authors.affiliation: Institute of Pathophysiology, "Ljubodrag Buba Mihailović", Faculty of Medicine, University of Belgrade, Belgrade, Serbia.
authors.forename: Dušan
authors.initials: D
authors.lastname: Mladenović
authors.affiliation: Institute for Medical Research, Military Medical Academy, Belgrade, Serbia.
authors.forename: Milica
authors.initials: M
authors.lastname: Ninković
authors.affiliation: Institute of Pathophysiology, "Ljubodrag Buba Mihailović", Faculty of Medicine, University of Belgrade, Belgrade, Serbia.
authors.forename: Vuk
authors.initials: V
authors.lastname: Aleksić
authors.affiliation: Clinics of Gynecology and Obstetrics, "Narodni front", Faculty of Medicine, University of Belgrade, Belgrade, Serbia.
authors.forename: Tamara
authors.initials: T
authors.lastname: Šljivančanin
authors.affiliation: Institute of Pathophysiology, "Ljubodrag Buba Mihailović", Faculty of Medicine, University of Belgrade, Belgrade, Serbia.
authors.forename: Danijela
authors.initials: D
authors.lastname: Vučević
authors.affiliation: Faculty of Dentistry,Pančevo, University of Business Economy, Novi Sad, Serbia.
authors.forename: Vera
authors.initials: V
authors.lastname: Todorović
authors.affiliation: Institute of Pathophysiology, "Ljubodrag Buba Mihailović", Faculty of Medicine, University of Belgrade, Belgrade, Serbia.
authors.forename: Milena
authors.initials: M
authors.lastname: Stanković
authors.affiliation: Institute of Medical Physiology, "Richard Burian", Faculty of Medicine, University of Belgrade, Belgrade, Serbia.
authors.forename: Olivera
authors.initials: O
authors.lastname: Stanojlović
authors.affiliation: Institute of Pathophysiology, "Ljubodrag Buba Mihailović", Faculty of Medicine, University of Belgrade, Belgrade, Serbia. Electronic address: tanjamm@med.bg.ac.rs.
authors.forename: Tatjana
authors.initials: T
authors.lastname: Radosavljević
mesh_terms: D000208:Acute Disease; D000818:Animals; D031204:Caloric Restriction; D004195:Disease Models, Animal; D000431:Ethanol; D005978:Glutathione / Q000378:metabolism; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008108:Liver Diseases, Alcoholic / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009566:Nitrates / Q000378:metabolism; D009573:Nitrites / Q000378:metabolism; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D026361:Reactive Nitrogen Species / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors
chemical_list: D009566:Nitrates; D009573:Nitrites; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species; D000431:Ethanol; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: Calorie restriction; Ethanol; Liver; Oxidative stress; Rats