title: Targeted ANP32E mutant mice do not demonstrate obvious movement defects.
abstract: BACKGROUND
  The ANP32 family of proteins have been implicated in neuronal function through biochemical and cellular biology studies in neurons, as well as by recent behavioural studies of a gene-trapped loss-of-function mutation of Anp32e in mice, particularly with respect to fine motor function. A second targeted allele of the Anp32e, however, did not appear to demonstrate neurological phenotypes.
  
  
  RESULTS
  Using a stringently controlled cohort of ten-generation backcrossed, co-caged, sex-matched, littermate pairs, we assayed for potential motor defects in the targeted ANP32E-deficient mice. We found no phenotypic difference in any assays.
  
  
  CONCLUSIONS
  Since it is unlikely that the gene-trap is a more complete loss-of-function, our results suggest that ANP32E has no appreciable effect on motor functions and that genetic background differences most likely account for the gene-trap phenomena.
authors.affiliation: Neuroscience and Behavioral Disorders Program, Duke-NUS Graduate Medical School, Singapore, Singapore.
authors.forename: Peiyan
authors.initials: P
authors.lastname: Wong
authors.forename: Vonny I
authors.initials: VI
authors.lastname: Leo
authors.forename: Meijun
authors.initials: M
authors.lastname: Low
authors.forename: Tak W
authors.initials: TW
authors.lastname: Mak
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zhang
authors.forename: Patrick T
authors.initials: PT
authors.lastname: Reilly
mesh_terms: D000483:Alleles; D000818:Animals; D005260:Female; D006579:Heterozygote; D006720:Homozygote; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D018832:Molecular Chaperones; D009043:Motor Activity / Q000502:physiology*; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000235:genetics*; D010641:Phenotype
chemical_list: C435730:Anp32e protein, mouse; D018832:Molecular Chaperones; D009419:Nerve Tissue Proteins