title: [Selection of reference genes for virulence gene expression in Vibrio parahaemolyticus].
abstract: OBJECTIVE
  We selected reference genes for virulence gene expression of Vibrio parahaemolyticus under different environmental conditions.
  
  
  METHODS
  Using qRT-PCR, we evaluated the expression stability of four housekeeping genes (GAPDH, pvuA, pvsA and rpoS) of V. parahaemolyticus cultivated separately in seawater, filtered seawater, shrimp and Tryptone Soya Broth.
  
  
  RESULTS
  The result shows that all the candidate reference genes could be amplified specifically in qRT-PCR reaction. The expression stability of the four reference genes ranked pvuA (2.906) > pvsA (3.197) > GAPDH (3.746) > rpoS (6.512). Further analysis with geNorm program reveals that the highest stability was observed in pvuA and pvsA. The geometric average score of the two genes was considered as the most appropriate reference gene for normalizing the expression of virulence genes of V. parahaemolyticus.
  
  
  CONCLUSIONS
  The pvuA and pvsA genes could be used as reference genes to study the virulence gene expression of V. parahaemolyticus.
authors.affiliation: College of Food Science and Technology, Shanghai Ocean University, Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai 201306, China. lqapple1988@yahoo.com.cn
authors.forename: Qin
authors.initials: Q
authors.lastname: Li
authors.forename: Zhiyun
authors.initials: Z
authors.lastname: Peng
authors.forename: Xinpeng
authors.initials: X
authors.lastname: Chen
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Sun
authors.forename: Yingjie
authors.initials: Y
authors.lastname: Pan
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhao
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D020869:Gene Expression Profiling / Q000592:standards; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D020043:Genes, Essential; D012015:Reference Standards; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012623:Seawater; D012984:Software; D014735:Vibrio Infections / Q000382:microbiology; D014736:Vibrio parahaemolyticus / Q000235:genetics* / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics
chemical_list: D001426:Bacterial Proteins