title: Analysis of the complete genome sequences of one swine and two human hepatitis E virus genotype 4 strains isolated in Beijing, China.
abstract: Full-length sequences were determined and analyzed for two human (MO and W3) and one swine (W2-5) hepatitis E virus (HEV) isolates from Beijing, China. The genomes of the three strains were composed of 7242, 7239, 7239 nucleotides, respectively, excluding the poly (A) tails, and were 84% identical to each other. All were classified into genotype 4. Sequence analysis shows that the 2 human isolates have up to 91-94% nucleotide identity in full length genome with swine strains isolated in China, while the swine isolate share 92% identity with the human strain T1 from Beijing. At the amino acid level, the three strains share 94%, 97% and 89-92% identity in the ORF1, ORF2 and ORF3, proteins respectively. The human strains MO and W3 have the highest identity, 97%, 98-99% and 96-98% in ORFs 1-3, respectively, to swine strains CHN-XJ-SW13 and CHN-XJ-SW33 from Xinjiang, China, while swine strain W2-5 has highest identity with the human strain HE-JA2, 96%, 99% and 91% in ORFs 1-3, respectively. Genotype specific amino acid substitutions were found at a single site in all three ORFs by sequences alignment, and genotype specific short sequences (5-10aa in length) were found in ORF1 and the C-terminus of ORF3. However, no difference was found at any amino acid position that discriminates between human and swine HEVs within genotype 4 for any of the three ORFs. These results indicated that the genotype 4 HEV strains from humans and pigs in China may evolve from the common ancestor.
authors.affiliation: Department of Cell Biology, National Institutes for Food and Drug Control, No. 2, Tiantanxili, Beijing 100050, China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Ma
authors.forename: Yansheng
authors.initials: Y
authors.lastname: Geng
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Li
authors.forename: Tim J
authors.initials: TJ
authors.lastname: Harrison
authors.forename: Weijin
authors.initials: W
authors.lastname: Huang
authors.forename: Chenyan
authors.initials: C
authors.lastname: Zhao
authors.forename: Youchun
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002681:China; D016679:Genome, Viral; D005838:Genotype; D016751:Hepatitis E / Q000662:veterinary* / Q000821:virology*; D016752:Hepatitis E virus / Q000145:classification / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D012367:RNA, Viral / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013552:Swine; D013553:Swine Diseases / Q000821:virology*
chemical_list: D012367:RNA, Viral
keywords: Complete genome; Hepatitis E virus; Sequence analysis; Swine HEV; Zoonotic transmission