title: High-molecular-weight poly-gamma-glutamate protects against hypertriglyceridemic effects of a high-fructose diet in rat.
abstract: We studied the effects of 2 different dosages of highmolecular- weight poly-γ-glutamic acid (hm γ-PGA) derived from Bacillus subtilis chungkookjang on lipid metabolism in a high-fructose diet-induced hypertriglyceridemic animal model. For 4 weeks, rats were fed either AIN-93 diet (normal control, NC; n = 10) or modified AIN-93 diet in which cornstarch was substituted with 63% fructose (n = 30) to induce hypertriglyceridemia. After 4 weeks, the hypertriglyceridemic rats were treated with daily oral doses of 0 mg (hypertriglyceridemic control, HC), 2.5 mg (hypertriglyceridemic, low hm γ-PGA, HL), or 5 mg·kg·bw(-1)·d(-1) (hypertriglyceridemic, high hm γ-PGA, HH) hm γ-PGA for 4 weeks. The HL and HH groups exhibited significantly lower levels of serum triglyceride, total cholesterol, LDL cholesterol, and free fatty acids than the HC group. The administration of hm γ-PGA reduced serum ALT and AST levels. The activities of lipogenic enzymes such as hepatic malic enzyme and glucose-6-phosphate dehydrogenase as well as glucose-6-phosphate dehydrogenase mRNA expression were significantly decreased by hm γ-PGA administration (p < 0.05). These results indicate that hm γ- PGA has an anti-hypertriglyceridemic effect in highfructose diet-induced hypertriglyceridemic rats.
authors.affiliation: Department of Food and Nutrition, Kookmin University, Seoul 136-702, Korea.
authors.forename: Yeong Hui
authors.initials: YH
authors.lastname: Jeon
authors.forename: Mi-Sun
authors.initials: MS
authors.lastname: Kwak
authors.forename: Moon-Hee
authors.initials: MH
authors.lastname: Sung
authors.forename: Sun-Hee
authors.initials: SH
authors.lastname: Kim
authors.forename: Myung-Hwan
authors.initials: MH
authors.lastname: Kim
authors.forename: Moon-Jeong
authors.initials: MJ
authors.lastname: Chang
mesh_terms: D000818:Animals; D001412:Bacillus subtilis / Q000737:chemistry; D001688:Biological Products / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D004032:Diet / Q000379:methods*; D005632:Fructose / Q000008:administration & dosage*; D008970:Molecular Weight; D011099:Polyglutamic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D051381:Rats; D014280:Triglycerides / Q000097:blood*
chemical_list: D001688:Biological Products; D014280:Triglycerides; C511775:poly(gamma-glutamic acid); D011099:Polyglutamic Acid; D005632:Fructose