title: Profiling celiac disease antibody repertoire.
abstract: The aim of this study was to dissect the autoantibody response in celiac disease (CD) that remains largely unknown, with the goal of identifying the disease-specific autoantigenic protein pattern or the so called epitome. Sera from CD patients were used to select immunoreactive antigens from a cDNA phage-display library. Candidate genes were identified, the corresponding proteins produced and their immunoreactivity validated with sera from CD patients and controls. Thirteen CD-specific antigens were identified and further validated by protein microarray. The specificity for 6 of these antigens was confirmed by ELISA. Furthermore we showed that this antibody response was not abolished on a gluten free diet and was not shared with other autoimmune diseases. These antigens appear to be CD specific and independent of gluten induction. The utility of this panel extends beyond its diagnostic value and it may drive the attention to new targets for unbiased screens in autoimmunity research.
authors.affiliation: Department of Health Sciences and Interdisciplinary Research Center on Autoimmune Diseases (IRCAD), University of Eastern Piedmont Amedeo Avogadro, Novara, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: D'Angelo
authors.forename: Flavio
authors.initials: F
authors.lastname: Mignone
authors.forename: Cecilia
authors.initials: C
authors.lastname: Deantonio
authors.forename: Roberto
authors.initials: R
authors.lastname: Di Niro
authors.forename: Roberta
authors.initials: R
authors.lastname: Bordoni
authors.forename: Roberto
authors.initials: R
authors.lastname: Marzari
authors.forename: Gianluca
authors.initials: G
authors.lastname: De Bellis
authors.forename: Tarcisio
authors.initials: T
authors.lastname: Not
authors.forename: Fortunato
authors.initials: F
authors.lastname: Ferrara
authors.forename: Andrew
authors.initials: A
authors.lastname: Bradbury
authors.forename: Claudio
authors.initials: C
authors.lastname: Santoro
authors.forename: Daniele
authors.initials: D
authors.lastname: Sblattero
mesh_terms: D000293:Adolescent; D000328:Adult; D001323:Autoantibodies / Q000097:blood / Q000276:immunology*; D001324:Autoantigens / Q000235:genetics / Q000276:immunology*; D002446:Celiac Disease / Q000097:blood / Q000175:diagnosis / Q000235:genetics / Q000276:immunology*; D061505:Cell Surface Display Techniques; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D040081:Protein Array Analysis; D012372:ROC Curve; D055815:Young Adult
chemical_list: D001323:Autoantibodies; D001324:Autoantigens