title: Tumor environmental factors glucose deprivation and lactic acidosis induce mitotic chromosomal instability--an implication in aneuploid human tumors.
abstract: Mitotic chromosomal instability (CIN) plays important roles in tumor progression, but what causes CIN is incompletely understood. In general, tumor CIN arises from abnormal mitosis, which is caused by either intrinsic or extrinsic factors. While intrinsic factors such as mitotic checkpoint genes have been intensively studied, the impact of tumor microenvironmental factors on tumor CIN is largely unknown. We investigate if glucose deprivation and lactic acidosis--two tumor microenvironmental factors--could induce cancer cell CIN. We show that glucose deprivation with lactic acidosis significantly increases CIN in 4T1, MCF-7 and HCT116 scored by micronuclei, or aneuploidy, or abnormal mitosis, potentially via damaging DNA, up-regulating mitotic checkpoint genes, and/or amplifying centrosome. Of note, the feature of CIN induced by glucose deprivation with lactic acidosis is similar to that of aneuploid human tumors. We conclude that tumor environmental factors glucose deprivation and lactic acidosis can induce tumor CIN and propose that they are potentially responsible for human tumor aneuploidy.
authors.affiliation: Cancer Institute (a Key Laboratory for Cancer Intervention and Prevention of China National Ministry of Education), The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Dai
authors.forename: Feifei
authors.initials: F
authors.lastname: Sun
authors.forename: Chunpeng
authors.initials: C
authors.lastname: Zhu
authors.forename: Xun
authors.initials: X
authors.lastname: Hu
mesh_terms: D000140:Acidosis, Lactic / Q000378:metabolism*; D000782:Aneuploidy; D000818:Animals; D045744:Cell Line, Tumor; D018385:Centrosome / Q000378:metabolism; D043171:Chromosomal Instability; D004249:DNA Damage; D004195:Disease Models, Animal; D005784:Gene Amplification; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D051379:Mice; D008938:Mitosis / Q000235:genetics*; D009369:Neoplasms / Q000235:genetics* / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: D005947:Glucose