title: Effect of type 1 herpes simplex infection of phenotypic peculiarities of human vascular endothelial cells in culture.
abstract: We studied the effect of type 1 herpes simplex infection on the production of innate immunity mediators in human vascular endothelial cells in culture. It was found that production of anti-inflammatory cytokines IL-1β, IL.6, and TNF-α in infected cultures depended on the level of their spontaneous production, while IL-8 production was suppressed irrespective of its spontaneous level. Shedding of cell adhesion molecules of early (P-selectin and E-selectin) and late (PECAM-1 and VE-cadherin) phases of leukocyte recruitment depended on individual capacity of human vascular endothelial cell cultures to maintain reproduction of type 1 herpes simples virus. The production of vasodilator NO and vasoconstrictor endothelin-1 by infected cultures also depended on spontaneous synthesis of this transmitter by non-infected cultures.
authors.affiliation: N. F. Gamaleya Institute of Epidemiology and Microbiology, Ministry of Health Care and Social Development of the Russian Federation, Moscow, Russia. scheglovitova@rambler.ru
authors.forename: O N
authors.initials: ON
authors.lastname: Scheglovitova
authors.forename: N N
authors.initials: NN
authors.lastname: Skliankina
authors.forename: N V
authors.initials: NV
authors.lastname: Boldyreva
authors.forename: A A
authors.initials: AA
authors.lastname: Babayants
authors.forename: I S
authors.initials: IS
authors.lastname: Frolova
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D015820:Cadherins / Q000378:metabolism; D002478:Cells, Cultured; D019040:E-Selectin / Q000378:metabolism; D042783:Endothelial Cells / Q000276:immunology* / Q000378:metabolism / Q000821:virology*; D019332:Endothelin-1 / Q000378:metabolism; D018259:Herpesvirus 1, Human / Q000276:immunology*; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D019007:P-Selectin / Q000378:metabolism; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D000099152:Cadherin 5
chemical_list: D015703:Antigens, CD; D015820:Cadherins; D019040:E-Selectin; D019332:Endothelin-1; D053583:Interleukin-1beta; D015850:Interleukin-6; D016209:Interleukin-8; D009569:Nitric Oxide; D019007:P-Selectin; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D014409:Tumor Necrosis Factor-alpha; D000099152:Cadherin 5