title: AXM mutagenesis: an efficient means for the production of libraries for directed evolution of proteins.
abstract: Affinity maturation is an important part of the recombinant antibody development process. There are several well-established approaches for generating libraries of mutated antibody genes for affinity maturation, but these approaches are generally too laborious or expensive to allow high-throughput, parallel processing of multiple antibodies. Here, we describe a scalable approach that enables the generation of libraries with greater than 10(8) clones from a single Escherichia coli transformation. In our method, a mutated DNA fragment is produced using PCR conditions that promote nucleotide misincorporation into newly synthesized DNA. In the PCR reaction, one of the primers contains at least three phosphorothioate linkages at its 5' end, and treatment of the PCR product with a 5' to 3' exonuclease is used to preferentially remove the strand synthesized with the non-modified primer, resulting in a single-stranded DNA fragment. This fragment then serves as a megaprimer to prime DNA synthesis on a uracilated, circular, single-stranded template in a Kunkel-like mutagenesis reaction that biases nucleotide base-changes between the megaprimer and uracilated DNA sequence in favor of the in vitro synthesized megaprimer. This method eliminates the inefficient subcloning steps that are normally required for the construction of affinity maturation libraries from randomly mutagenized antibody genes.
authors.affiliation: AxioMx, Inc., 688 E. Main St., Branford, CT 06405, USA.
authors.forename: Erika G
authors.initials: EG
authors.lastname: Holland
authors.forename: Diane L
authors.initials: DL
authors.lastname: Buhr
authors.forename: Felicity E
authors.initials: FE
authors.lastname: Acca
authors.forename: Dawn
authors.initials: D
authors.lastname: Alderman
authors.forename: Kristin
authors.initials: K
authors.lastname: Bovat
authors.forename: Valeria
authors.initials: V
authors.lastname: Busygina
authors.forename: Brian K
authors.initials: BK
authors.lastname: Kay
authors.forename: Michael P
authors.initials: MP
authors.lastname: Weiner
authors.forename: Margaret M
authors.initials: MM
authors.lastname: Kiss
mesh_terms: D004926:Escherichia coli / Q000235:genetics; D016296:Mutagenesis; D019151:Peptide Library; D016133:Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000096:biosynthesis*
chemical_list: D019151:Peptide Library; D011994:Recombinant Proteins
keywords: Affinity maturation; Directed evolution; Phage display; Recombinant antibodies