title: A functional autophagy pathway is required for rapamycin-induced degradation of the Sgs1 helicase in Saccharomyces cerevisiae.
abstract: In yeast Saccharomyces cerevisiae, the immunosuppressant rapamycin mimics starvation by inhibiting the kinase Tor1. We recently documented that this treatment triggers a rapid degradation of Sgs1, a helicase involved in several biological processes such as the prevention of genomic instability. Herein, we show that yeast strains deleted for genes ATG2, ATG9, and PEP4, encoding components of the autophagy pathway, prevent rapamycin-induced degradation of Sgs1. We propose that defects in the autophagy pathway prevent degradation of key proteins in the rapamycin response pathway and as a consequence cause resistance to the drug.
authors.affiliation: University of Carthage, High Institute of Environmental Sciences and Technologies, Borj-Cedria Science and Technology Park, PB-1003, Hammam-Lif 2050, Tunisia.
authors.forename: Rim
authors.initials: R
authors.lastname: Marrakchi
authors.forename: Chedly
authors.initials: C
authors.lastname: Chouchani
authors.forename: Jeremie
authors.initials: J
authors.lastname: Poschmann
authors.forename: Emil
authors.initials: E
authors.lastname: Andreev
authors.forename: Mohamed
authors.initials: M
authors.lastname: Cherif
authors.forename: Dindial
authors.initials: D
authors.lastname: Ramotar
mesh_terms: D001343:Autophagy; D001483:Base Sequence; D017931:DNA Primers; D059748:Proteolysis; D053484:RecQ Helicases / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000201:enzymology / Q000276:immunology; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D020123:Sirolimus / Q000494:pharmacology*
chemical_list: D017931:DNA Primers; D029701:Saccharomyces cerevisiae Proteins; C090472:SGS1 protein, S cerevisiae; D053484:RecQ Helicases; D020123:Sirolimus