title: Sequences of metanicins, 20-residue peptaibols from the ascomycetous fungus CBS 597.80.
abstract: Four linear 20-residue peptaibols, named metanicins (MTCs) A-D, were isolated from submerged cultures of the ascomycetous fungus CBS 597.80. Structure elucidation was performed by a combination of fast-atom-bombardment mass spectrometry (FAB-MS), electrospray ionization MS, Edman degradation of isolated fragments, and amino acid analysis by ion-exchange and gas chromatography, and enantioselective HPLC. The sequences of MTC A(B) are (amino acid exchange in B and C in parentheses): Ac-Aib-Ala-Aib-Ala-Aib-Ala-Gln-Aib-Val-Aib-Gly-Leu-Aib-Pro-Val-Aib-Aib(D-Iva)-Gln-Gln-Pheol and of MTC C(D) Ac-Aib-Ala-Aib-Ala-Aib-Ala-Gln-Aib-Val-Aib-Gly-Leu-Aib-Pro-Val-Aib-Aib(D-Iva)-Gln-Gln-Pheol (Ac, acetyl; Aib, α-aminoisobutyric acid; Iva, isovaline; Pheol, L-phenylalaninol). The peptides are related, and some of the sequences are identical, to other 20-residue peptaibols isolated from Trichoderma species. MTCs show moderate activities against Micrococcus luteus, Enterococcus faecalis, and Staphylococcus aureus, and very low activities against Bacillus subtilis. The producer has originally been identified and deposited as Metarhizium anisopliae var. anisopliae CBS 597.80. Although this identification has been withdrawn by Centralbureau voor Schimmelcultures (CBS) in the meantime, the accession number will be retained - independently from any taxonomic revisions.
authors.affiliation: Interdisciplinary Research Centre for BioSystems, Land Use and Nutrition IFZ, Institute of Nutritional Science, Department of Food Sciences, University of Giessen, Heinrich-Buff-Ring 26 - 32, D-35392 Giessen. akimonyo@gmx.de
authors.forename: Anastase
authors.initials: A
authors.lastname: Kimonyo
authors.forename: Hans
authors.initials: H
authors.lastname: Brückner
mesh_terms: D001203:Ascomycota; D002851:Chromatography, High Pressure Liquid; D054848:Peptaibols / Q000737:chemistry* / Q000235:genetics
chemical_list: D054848:Peptaibols