title: Influence of curing agent on fibrosis around silicone implants.
abstract: Severe capsular contracture around silicone expander breast implants leading to pain and failure is a major clinical problem. Even though earlier studies have implicated the immunogenicity of silicone, the role of physical and chemical properties of the silicone material in excessive collagen deposition and fibrosis has been less addressed. The present study investigates whether there is any correlation between the type of curing systems i.e. addition and free radical curing and the fibrosis around silicone elastomer. The experiment carried out uses commercially available silicone ventriculo-peritoneal shunt material elastomer cured by platinum and the results are compared with results obtained in a similar study carried out by the authors using commercially available silicone tissue expander material cured by peroxide. Ultra-high molecular weight poly-ethylene (UHMWPE), the standard reference for biocompatibility evaluation, was used as the control material. The materials were implanted in rat skeletal muscle for 30 and 90 days. Inflammatory cells, myofibroblasts, cytokines, and collagen deposition at the material-tissue interface were identified by haematoxylin-eosin and Masson's Trichrome stains and semi-quantitated based on immunohistochemical studies. Results indicate that even though the cellular response in the initial phase of wound healing was similar in both platinum and peroxide-cured materials, the collagen deposition in the proliferative phase was more around peroxide-cured material in comparison to the platinum-cured silicone elastomer. There is a need to look into the molecular mechanisms of this interaction and the possibility of using curing systems other than free radical peroxide in the manufacture of silicone elastomer expanders for breast prosthesis.
authors.affiliation: Division of Implant Biology, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Trivandrum, Kerala 695012, India.
authors.forename: Josna
authors.initials: J
authors.lastname: Joseph
authors.forename: Mira
authors.initials: M
authors.lastname: Mohanty
mesh_terms: D000269:Adhesives / Q000009:adverse effects / Q000494:pharmacology*; D000818:Animals; D019928:Breast Implantation / Q000009:adverse effects / Q000379:methods; D018427:Breast Implants / Q000009:adverse effects*; D005347:Fibroblasts / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D005355:Fibrosis / Q000139:chemically induced / Q000276:immunology; D005549:Foreign-Body Reaction / Q000276:immunology / Q000473:pathology; D008214:Lymphocytes / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D008422:Materials Testing; D008970:Molecular Weight; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000473:pathology*; D020959:Polyethylene / Q000737:chemistry / Q000276:immunology; D051381:Rats; D012826:Silicone Elastomers / Q000009:adverse effects / Q000737:chemistry; D012828:Silicones / Q000009:adverse effects*
chemical_list: D000269:Adhesives; D012826:Silicone Elastomers; D012828:Silicones; D020959:Polyethylene