title: Heliimonas saccharivorans gen. nov., sp. nov., a member of the family Chitinophagaceae isolated from a mineral water aquifer, and emended description of Filimonas lacunae.
abstract: Two isolates, with optimum growth temperature and pH of about 30 °C and 6.0-7.0, were recovered from a borehole head of a mineral water aquifer in Portugal. The closest relatives based on 16S rRNA gene sequence analysis were species of genera of the family Chitinophagaceae. Strains L2-4(T) and L2-109 formed translucent colonies and non-motile pleomorphic cells. Strains were strictly aerobic, and oxidase- and catalase-positive. The major fatty acids of strains L2-4(T) and L2-109 were 17 : 0 iso 3-OH, 15 : 0 iso and 15 : 1 iso G. The major polar lipids were phosphatidylethanolamine, one unidentified phospholipid, four unidentified aminophospholipids, four unidentified aminolipids and three unidentified polar lipids. Menaquinone 7 was the only respiratory quinone. The G+C content of the DNA of strains L2-4(T) and L2-109 was 42.0 and 41.4 mol%, respectively. Based on 16S rRNA gene sequence analysis, physiological and biochemical characteristics, strains L2-4(T) ( = CECT 8122(T) = LMG 26919(T)) and L2-109 ( = CECT 8121 = LMG 26920) are considered to represent a novel species of a new genus, for which the name Heliimonas saccharivorans gen. nov., sp. nov. is proposed. The type strain of Heliimonas saccharivorans is L2-4(T). Due to additional results obtained in this study an emended description of Filimonas lacunae is provided.
authors.affiliation: Center for Neuroscience and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal.
authors.forename: Tânia
authors.initials: T
authors.lastname: Leandro
authors.affiliation: Center for Neuroscience and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal.
authors.forename: Luís
authors.initials: L
authors.lastname: França
authors.affiliation: Department of Life Sciences, University of Coimbra, 3001-401 Coimbra, Portugal.|Center for Neuroscience and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal.
authors.forename: M Fernanda
authors.initials: MF
authors.lastname: Nobre
authors.affiliation: Department of Biological Sciences, University of Alaska Anchorage, Anchorage, AK 99508, USA.
authors.forename: Fred A
authors.initials: FA
authors.lastname: Rainey
authors.affiliation: Department of Life Sciences, University of Coimbra, 3001-401 Coimbra, Portugal.
authors.forename: Milton S
authors.initials: MS
authors.lastname: da Costa
mesh_terms: D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D060587:Groundwater / Q000382:microbiology*; D008900:Mineral Waters / Q000382:microbiology*; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D011174:Portugal; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D008900:Mineral Waters; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7