title: Changes of phenolic secondary metabolite profiles in the reaction of narrow leaf lupin (Lupinus angustifolius) plants to infections withColletotrichum lupinifungus or treatment with its toxin.
abstract: Plant interactions with environmental factors cause changes in the metabolism and regulation of biochemical and physiological processes. Plant defense against pathogenic microorganisms depends on an innate immunity system that is activated as a result of infection. There are two mechanisms of triggering this system: basal immunity activated as a result of a perception of microbe-associated molecular patterns through pattern recognition receptors situated on the cell surface and effector-triggered immunity (ETI). An induced biosynthesis of bioactive secondary metabolites, in particular phytoalexins, is one of the mechanisms of plant defense to fungal infection. Results of the study on narrow leaf lupin (Lupinus angustifoliusL.) plants infected with the anthracnose fungusColletotrichum lupiniand treated with fungal phytotoxic metabolites are described in the paper. TheC. lupiniphytotoxins were isolated from liquid cultures, purified and partially characterized with physicochemical methods. Accumulation of secondary metabolites on leaf surface and within the tissues of plants either infected, treated with the fungal phytotoxin or submitted to both treatments was studied using GC-MS and LC-MS, respectively. Substantial differences in isoflavone aglycones and glycoconjugate profiles occurred in response to different ways of plant treatment.
authors.affiliation: Institute of Bioorganic Chemistry PAS, Noskowskiego 12/14, 61-704 Poznań, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Wojakowska
authors.forename: Dorota
authors.initials: D
authors.lastname: Muth
authors.forename: Dorota
authors.initials: D
authors.lastname: Narożna
authors.forename: Cezary
authors.initials: C
authors.lastname: Mądrzak
authors.forename: Maciej
authors.initials: M
authors.lastname: Stobiecki
authors.forename: Piotr
authors.initials: P
authors.lastname: Kachlicki
keywords: Colletotrichum lupini; Elicitor; Flavonoids; Infection; Liquid chromatography/Mass spectrometry; Lupinus angustifolius; Secondary metabolite profiling