title: Development of test strips for rapid buprenorphine detection in vitro.
abstract: OBJECTIVE
  Buprenorphine (BUP) is the primary treatment for narcotic addiction, but it is often abused by opioid-dependent patients in many countries. For timely and effective detection and controlling the amount of BUP used in therapy, a rapid and sensitive test is needed. In the present study, we describe the development of test strips using monoclonal antibodies (MAbs) for the detection of BUP.
  
  
  METHODS
  The MAbs were generated from hybridomas, and purified MAbs were used to create colloidal gold-antibody conjugates that were placed in the test strips.
  
  
  RESULTS
  The BUP test strips had a limit of detection (LOD) of 12.5 ng/mL and did not cross-react with other drugs tested at physiological levels.
  
  
  CONCLUSIONS
  Therefore, this assay has sufficient sensitivity and specificity for BUP detection in urine specimens so that the dosage of BUP given to individuals being treated for opioid dependence can be monitored.
authors.affiliation: The Pneumology Department of the Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.
authors.forename: Sheng-Jin
authors.initials: SJ
authors.lastname: Li
authors.affiliation: The Laboratory Medicine Department of the Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, China.
authors.forename: Ding
authors.initials: D
authors.lastname: Wang
authors.affiliation: The Institute for Viral Hepatitis, Key Laboratory of Molecular Biology on Infectious Diseases of the Ministry of Education, Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: The Institute for Viral Hepatitis, Key Laboratory of Molecular Biology on Infectious Diseases of the Ministry of Education, Chongqing Medical University, Chongqing, 400016, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zheng
authors.affiliation: The Laboratory Medicine Department of the Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, China. Electronic address: ache11@163.com.
authors.forename: Wei-Xian
authors.initials: WX
authors.lastname: Chen
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D002047:Buprenorphine / Q000652:urine*; D002624:Chemistry, Clinical / Q000379:methods*; D002846:Chromatography, Affinity; D004591:Electrophoresis, Polyacrylamide Gel; D006112:Gravitation; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D051379:Mice; D008807:Mice, Inbred BALB C; D011934:Reagent Strips
chemical_list: D000911:Antibodies, Monoclonal; D011934:Reagent Strips; D002047:Buprenorphine
keywords: 3,4-Methylenedioxyethylamphetamine; BCOCB; BSA; BTG; BUP; BUP-Gluc; Bovine serum albumin; Bovine thyroglobulin; Buprenorphine; Buprenorphine-3-β-d-glucuronide; Colloidal gold; ELISA; Enzoylecgonine; Enzyme-linked immunosorbent assay; GC; Gas chromatography; LC; Liquid chromatography; MAbs; MDEA; MS; Mass spectrometry; Monoclonal antibodies; Monoclonal antibody; NBUP; NBUP-Gluc; Norbuprenorphine; Norbuprenorphine-3-β-d-glucuronide; OD; Optical densities; PBS; Phosphate-buffered saline; SD; Standard deviations; THC; TMB; Test strips; Tetramethylbenzidine; ∆-9 Tetrahydrocannabinol