title: Bovine carriers of Anaplasma marginale and Anaplasma bovis in South India.
abstract: Carriers of bovine anaplasmosis in Northern Kerala, South India were detected using conventional microscopical and molecular techniques. PCR-RFLP and nested PCR techniques were used for detection of Anaplasma marginale and Anaplasma bovis respectively and the PCR products were confirmed by sequencing. Out of 150 samples tested, 25 were detected positive for A. marginale and five for A. bovis based on molecular tests. The inclusion bodies of A. marginale could be detected by microscopy in two blood smears after staining by giemsa while acridine orange staining detected three smears positive. The data clearly suggest the higher sensitivity of molecular techniques for diagnosis of these diseases.
authors.affiliation: Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala, India.
authors.forename: A S
authors.initials: AS
authors.lastname: Nair
authors.forename: R
authors.initials: R
authors.lastname: Ravindran
authors.forename: B
authors.initials: B
authors.lastname: Lakshmanan
authors.forename: C
authors.initials: C
authors.lastname: Sreekumar
authors.forename: S S
authors.initials: SS
authors.lastname: Kumar
authors.forename: Remya
authors.initials: R
authors.lastname: Raju
authors.forename: P V
authors.initials: PV
authors.lastname: Tresamol
authors.forename: M B
authors.initials: MB
authors.lastname: Vimalkumar
authors.forename: M R
authors.initials: MR
authors.lastname: Saseendranath
mesh_terms: D000709:Anaplasma / Q000302:isolation & purification*; D000712:Anaplasmosis / Q000453:epidemiology* / Q000382:microbiology*; D000818:Animals; D001431:Bacteriological Techniques; D001769:Blood / Q000382:microbiology; D002353:Carrier State / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology* / Q000382:microbiology*; D003430:Cross-Sectional Studies; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D007194:India; D008853:Microscopy; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015995:Prevalence; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S