title: Neural induction with a dopaminergic phenotype from human pluripotent stem cells through a feeder-free floating aggregation culture.
abstract: Pluripotent stem cells are promising potential sources for cell replacement therapy and are useful research tools for exploring disease mechanisms. Neural cells are one of the cell types that have been most efficiently differentiated through several established protocols. This chapter describes the feeder-free floating aggregation culture system for the induction of dopaminergic neurons. This method is simple and highly efficient for the production of dopaminergic neurons. It has several advantages for application in clinical usage in comparison to the other protocols using either feeder cells or Matrigel.
authors.affiliation: Department of Clinical Application, Center for iPS Cell, Kyoto University, Kyoto, Japan.
authors.forename: Asuka
authors.initials: A
authors.lastname: Morizane
authors.forename: Daisuke
authors.initials: D
authors.lastname: Doi
authors.forename: Jun
authors.initials: J
authors.lastname: Takahashi
mesh_terms: D002448:Cell Adhesion / Q000187:drug effects; D002449:Cell Aggregation / Q000187:drug effects; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation / Q000187:drug effects; D003470:Culture Media / Q000494:pharmacology; D059290:Dopaminergic Neurons / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D061252:Feeder Cells / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D007150:Immunohistochemistry; D010641:Phenotype; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors
chemical_list: D003470:Culture Media