title: Rapid, whole blood diagnostic test for detecting anti-hantavirus antibody in rats.
abstract: Hantavirus is a causative agent of rodent-borne viral zoonoses, hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome. Seoul virus (SEOV) is a causative agent of urban and laboratory rat-associated HFRS worldwide. Surveillance of rodents has been done mainly by serological detection of hantavirus-specific antibodies by enzyme linked immunosorbent assay (ELISA) and immunofluorescent antibody assay (IFA). An immunochromatographic (ICG) test was developed with the N-terminal 103 amino acids of nucleocapsid protein of Hantaan virus expressed by Escherichia coli as an antigen to detect IgG antibody specific to hantavirus in sera from Rattus sp. animals. Antibody-detecting sensitivity of the ICG test was the same as that of ELISA and about 100-times higher than that of IFA. Overall sensitivities and specificities of the ICG test in comparison to ELISA and IFA for sera from 192 urban rats and 123 laboratory rats were 99.3% and 100%, respectively. Diluted whole blood samples without separation could be used for the ICG test. The ICG test enabled detection of antibodies to SEOV, Hantaan, Dobrava/Belgrade, and Thailand viruses, which are causative agents of HFRS throughout Eurasia. The ICG test is a rapid, simple and safe method for diagnosis of SEOV infection in rats.
authors.affiliation: Department of Microbiology, Graduate School of Medicine, Hokkaido University, Kita-ku, Kita-15, Nishi-7, Sapporo 060-8638, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Amada
authors.forename: Kumiko
authors.initials: K
authors.lastname: Yoshimatsu
authors.forename: Shumpei P
authors.initials: SP
authors.lastname: Yasuda
authors.forename: Kenta
authors.initials: K
authors.lastname: Shimizu
authors.forename: Takaaki
authors.initials: T
authors.lastname: Koma
authors.forename: Nobuhito
authors.initials: N
authors.lastname: Hayashimoto
authors.forename: Chandika D
authors.initials: CD
authors.lastname: Gamage
authors.forename: Sanae
authors.initials: S
authors.lastname: Nishio
authors.forename: Akira
authors.initials: A
authors.lastname: Takakura
authors.forename: Jiro
authors.initials: J
authors.lastname: Arikawa
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D036022:Capsid Proteins / Q000235:genetics; D002846:Chromatography, Affinity / Q000379:methods*; D003955:Diagnostic Tests, Routine / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D018063:Hantaan virus / Q000235:genetics / Q000276:immunology; D006476:Orthohantavirus / Q000276:immunology*; D018778:Hantavirus Infections / Q000276:immunology / Q000662:veterinary*; D007074:Immunoglobulin G / Q000097:blood; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics; D012376:Rodent Diseases / Q000175:diagnosis* / Q000276:immunology; D012680:Sensitivity and Specificity; D013785:Thailand; D014758:Viral Core Proteins / Q000235:genetics
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D014758:Viral Core Proteins; C111782:nucleocapsid protein, Hantaan virus
keywords: HFRS; Hemorrhagic fever with renal syndrome; ICG test; IgG; Laboratory animals; Seoul virus