title: Acupuncture suppresses reinstatement of morphine-seeking behavior induced by a complex cue in rats.
abstract: Morphine causes physical and psychological dependence for individuals after repeated-use. Above all, our previous study showed that acupuncture attenuated reinstatement of morphine-seeking behavior induced by pharmacological cue. In this study, we investigated whether acupuncture could suppress the reinstatement of morphine-seeking behavior induced by the combination of environmental and pharmacological cues and the possible neuronal involvement. Male Sprague-Dawley rats were trained to self-administer morphine (1.0 mg/kg) for 3 weeks. Following the withdrawal phase (7 days), the effects of acupuncture on reinstatement of morphine-seeking behavior were investigated. For the investigation of neuronal involvement, the GABAA receptor antagonist bicuculline and the GABAB receptor antagonist SCH 50911 were pre-treated. Morphine-seeking behavior induced by combination of re-exposure to the operant chamber and morphine injection was suppressed perfectly by acupuncture at SI5, but not at the control acupoint LI5 and this effect was blocked by pre-treatment with the GABA receptor antagonists. This study suggests that acupuncture at SI5 can be considered as a predominant therapy for the reinstatement of morphine-seeking behavior in humans.
authors.affiliation: Department of Acupuncture, Moxibustion and Acupoint, College of Oriental Medicine, Daegu Haany University, Daegu 706-828, South Korea. dlqhdgy@dhu.ac.kr
authors.forename: Bong Hyo
authors.initials: BH
authors.lastname: Lee
authors.forename: Sung Chul
authors.initials: SC
authors.lastname: Lim
authors.forename: Hyeon Jeong
authors.initials: HJ
authors.lastname: Jeon
authors.forename: Jae Su
authors.initials: JS
authors.lastname: Kim
authors.forename: Yun Kyu
authors.initials: YK
authors.lastname: Lee
authors.forename: Hyun Jong
authors.initials: HJ
authors.lastname: Lee
authors.forename: Sunghyun
authors.initials: S
authors.lastname: In
authors.forename: Hee Young
authors.initials: HY
authors.lastname: Kim
authors.forename: Seong Shoon
authors.initials: SS
authors.lastname: Yoon
authors.forename: Chae Ha
authors.initials: CH
authors.lastname: Yang
mesh_terms: D015670:Acupuncture Therapy / Q000379:methods*; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology*; D003216:Conditioning, Operant / Q000187:drug effects / Q000502:physiology*; D003463:Cues; D005108:Extinction, Psychological / Q000187:drug effects / Q000502:physiology*; D008297:Male; D009020:Morphine / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012646:Self Administration
chemical_list: D009020:Morphine
keywords: Acupuncture; Cue; Environment; GABA; Morphine; Reinstatement