title: Lower expression of ER-α36 is associated with the development of endometrial hyperplasia in PCOS patients.
abstract: To explore the expression change of ER-α36 in endometria of PCOS patients.
  
  
  
  Proliferative endometria were collected and divided into three groups: CE group (n=30), proliferative endometria from control women; PCOSE group (n=30), proliferative endometria from PCOS patients; HPCOSE group (n=15), hyperplastic endometria from PCOS patients. The cellular localization of ER-α36 and ER-α66 was analyzed by immunohistochemistry, and expression of ER-α36 and ER-α66 was determined by immunohistochemistry and Real-Time Quantitative PCR. The correlation between serum hormone concentration and expression of ER-α36 and ER-α66 was analyzed.
  
  
  
  ER-α36 was localized on the cell surface, cytoplasm and nucleus of glandular epithelial cells of both CE and PCOSE groups, mainly on the cell surface, and ER-α66 was localized on the nucleus. The protein and mRNA expression of ER-α36 was decreased from CE, PCOSE to HPCOSE group, while expression of ER-α66 showed no obvious difference among groups. The expression ratio of ER-α36 to ER-α66 of CE, PCOSE and HPCOSE group showed a decreasing tendency. The expression of ER-α36 and its expressive ratio to ER-α66 was negatively correlated with serum concentration of LH, LH/FSH, testosterone and androstenedione.
  
  
  
  Lower expression of ER-α66 is associated with the development of endometrial hyperplasia in PCOS patients.
authors.affiliation: Reproductive Medical Centre, Department of Obstetrics and Gynecology, Peking University Third Hospital, and Key Laboratory of Assisted Reproduction, Ministry of Education, and Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Beijing, China.
authors.forename: Sheng-Li
authors.initials: SL
authors.lastname: Lin
authors.forename: Bin-Bin
authors.initials: BB
authors.lastname: Tu
authors.forename: Xiao-Guo
authors.initials: XG
authors.lastname: Du
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Yan
authors.forename: Jie
authors.initials: J
authors.lastname: Qiao
mesh_terms: D004714:Endometrial Hyperplasia / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D047628:Estrogen Receptor alpha / Q000032:analysis / Q000096:biosynthesis*; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D007150:Immunohistochemistry; D018613:Microscopy, Confocal; D011085:Polycystic Ovary Syndrome / Q000150:complications / Q000378:metabolism* / Q000473:pathology; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D047628:Estrogen Receptor alpha