title: The PhosphoGRID Saccharomyces cerevisiae protein phosphorylation site database: version 2.0 update.
abstract: PhosphoGRID is an online database that curates and houses experimentally verified in vivo phosphorylation sites in the Saccharomyces cerevisiae proteome (www.phosphogrid.org). Phosphosites are annotated with specific protein kinases and/or phosphatases, along with the condition(s) under which the phosphorylation occurs and/or the effects on protein function. We report here an updated data set, including nine additional high-throughput (HTP) mass spectrometry studies. The version 2.0 data set contains information on 20 177 unique phosphorylated residues, representing a 4-fold increase from version 1.0, and includes 1614 unique phosphosites derived from focused low-throughput (LTP) studies. The overlap between HTP and LTP studies represents only ∼3% of the total unique sites, but importantly 45% of sites from LTP studies with defined function were discovered in at least two independent HTP studies. The majority of new phosphosites in this update occur on previously documented proteins, suggesting that coverage of phosphoproteins in the yeast proteome is approaching saturation. We will continue to update the PhosphoGRID data set, with the expectation that the integration of information from LTP and HTP studies will enable the development of predictive models of phosphorylation-based signaling networks. Database URL: http://www.phosphogrid.org/
authors.affiliation: Department of Biochemistry and Molecular Biology, Molecular Epigenetics, Life Sciences Institute, University of British Columbia, 2350 Health Sciences Mall, Vancouver, British Columbia, Canada V6T 1Z3. ijs.ubc@gmail.com
authors.forename: Ivan
authors.initials: I
authors.lastname: Sadowski
authors.forename: Bobby-Joe
authors.initials: BJ
authors.lastname: Breitkreutz
authors.forename: Chris
authors.initials: C
authors.lastname: Stark
authors.forename: Ting-Cheng
authors.initials: TC
authors.lastname: Su
authors.forename: Matthew
authors.initials: M
authors.lastname: Dahabieh
authors.forename: Sheetal
authors.initials: S
authors.lastname: Raithatha
authors.forename: Wendy
authors.initials: W
authors.lastname: Bernhard
authors.forename: Rose
authors.initials: R
authors.lastname: Oughtred
authors.forename: Kara
authors.initials: K
authors.lastname: Dolinski
authors.forename: Kris
authors.initials: K
authors.lastname: Barreto
authors.forename: Mike
authors.initials: M
authors.lastname: Tyers
mesh_terms: D030562:Databases, Protein; D057166:High-Throughput Screening Assays; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D020543:Proteome / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D010750:Phosphoproteins; D020543:Proteome; D029701:Saccharomyces cerevisiae Proteins