title: Differential expression of cell adhesion molecules in an ionizing radiation-induced breast cancer model system.
abstract: Cell-cell adhesion is mediated by members of the cadherin-catenin system and among them E-cadherin and β-catenin are important adhesion molecules for epithelial cell function and preservation of tissue integrity. To investigate the importance of cell adhesion molecules in breast carcinogenesis, we developed an in vitro breast cancer model system wherein immortalized human breast epithelial cell line, MCF-10F, was malignantly transformed by exposure to low doses of high linear energy transfer (LET) α particle radiation (150 keV/µm) and subsequent growth in the presence or absence of 17β-estradiol. This model consisted of human breast epithelial cells in different stages of transformation: i) parental cell line MCF-10F; ii) MCF-l0F continuously grown with estradiol at 10(-8) (Estrogen); iii) a non-malignant cell line (Alpha3); and iv) a malignant and tumorigenic cell line (Alpha5) and the Tumor2 cell line derived from the nude mouse xenograft of the Alpha5 cell line. Expression levels of important cell adhesion molecules such as α-catenin, β-catenin, γ-catenin, E-cadherin and integrin were found to be higher at the protein level in the Alpha5 and Tumor2 cell lines relative to these levels in the non-tumorigenic MCF-10F, Estrogen and Alpha3 cell lines. In corroboration, cDNA expression analysis revealed elevated levels of genes involved in the cell adhesion function [E-cadherin, integrin β6 and desmocollin3 (DSc3)] in the Alpha5 and Tumor2 cell lines relative to the levels in the MCF-10F, Estrogen and Alpha3 cell lines. Collectively, our results suggest that cell adhesion molecules are expressed at higher levels in malignantly transformed breast epithelial cells relative to levels in non-malignant cells. However, reduced levels of adhesion molecules observed in the mouse xenograft-derived Tumor 2 cell line compared to the pre-tumorigenic Alpha5 cell line suggests that the altered expression levels of adhesion molecules depend on the tumor tissue microenvironment.
authors.affiliation: Institute for Advanced Research, Tarapacá University, Arica, Chile. gmc24@columbia.edu
authors.forename: Gloria M
authors.initials: GM
authors.lastname: Calaf
authors.forename: Debasish
authors.initials: D
authors.lastname: Roy
authors.forename: Gopeshwar
authors.initials: G
authors.lastname: Narayan
authors.forename: Adayabalam S
authors.initials: AS
authors.lastname: Balajee
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000378:metabolism*; D015820:Cadherins / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000096:biosynthesis* / Q000378:metabolism*; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic / Q000528:radiation effects; D051187:Desmocollins; D005260:Female; D064593:Heterografts; D006801:Humans; D039641:Integrin beta Chains / Q000378:metabolism; D008562:Membrane Glycoproteins / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D009381:Neoplasms, Radiation-Induced / Q000378:metabolism*; D011839:Radiation, Ionizing; D059016:Tumor Microenvironment; D051178:alpha Catenin / Q000378:metabolism; D051176:beta Catenin / Q000378:metabolism; D051185:gamma Catenin / Q000378:metabolism
chemical_list: D015820:Cadherins; D015815:Cell Adhesion Molecules; D051187:Desmocollins; D039641:Integrin beta Chains; D008562:Membrane Glycoproteins; D051178:alpha Catenin; D051176:beta Catenin; D051185:gamma Catenin; C072170:integrin beta6; C090171:Dsc3 protein, mouse