title: Gene transfer in developing chick embryos: in ovo electroporation.
abstract: In ovo electroporation is a popular technique to study gene function during development. This technique enables precise temporal and spatial genetic manipulation with the added advantages of being quick and inexpensive. In this chapter the transient transfection of a construct into the neural tube of a chicken embryo via in ovo electroporation is described. Modifications of this basic technique and methods to -analyze the resulting electroporated embryos such as qPCR and microarray are also discussed.
authors.affiliation: Division of Genetics, Genomics and Development Center for Integrative Genomics, Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.
authors.forename: Emma K
authors.initials: EK
authors.lastname: Farley
mesh_terms: D000818:Animals; D000906:Antibodies / Q000378:metabolism; D002642:Chick Embryo; D018274:Electroporation / Q000379:methods*; D018014:Gene Transfer Techniques; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D013194:Staining and Labeling
chemical_list: D000906:Antibodies