title: Effect of Bizhongxiao Decotion (BZXD) on Some Cytokines in Plasma of Rats with CII-induced Rheumatoid Arthritis.
abstract: OBJECTIVE
  To investigate the influence of bizhongxiao decoction (BZXD) which is a Traditional Chinese medicine for RA including, on the plasma TNF-α and IL-1β in rats with CII-induced arthritis (CIA) and explore the protective mechanism of BZXD in the treatment of rheumatoid arthritis.
  
  
  METHODS
  75 SD rats were divided into four groups randomly. Normal control group (n=5) not be treated any more. The CIA rat was established by subcutaneous injection with bovine II collagen (B II C) and complete Freund, s adjuvant (CFA) after 7d breeding. The CIA rats were divided into the CIA group (n=16), BZXD group (n=29) treated with BZXD and the MTX group (n=25) treated with methotrexate. All rats were killed after various intervals (25, 30, 35, 40, or 45d). At the end of each time interval, we collected the blood of each rat. To detect TNF-α and IL-1β in plasma with radio-immunity kit.
  
  
  RESULTS
  BIIC and CFA can be used to copying CIA model. The incidence of arthritis was 88%. The plasma TNF-α and IL-1β levels of CIA group, BZXD group and MTX group were notably higher than those of normal control group (p<0.05), moreover, the CIA group was higher than those of the MTX group and BZXD group at various interval (p<0.01). TNF-α and IL-1β rose step by step in CIA group but decreased in BZXD group and MTX group gradually. Moreover, in BZXD group were lower than those in MTX group (p<0.05).
  
  
  CONCLUSIONS
  TNF-α and IL-1β play a very important role in the formation and development of RA. BZXD can notably decrease the plasma TNF-α and IL-1β levels, which was better than MTX.
authors.affiliation: Xiangya Hospital, Central South University, Changsha, China.
authors.forename: An-Yu
authors.initials: AY
authors.lastname: Wang
authors.forename: Qing-Hua
authors.initials: QH
authors.lastname: Liang
authors.forename: Xu
authors.initials: X
authors.lastname: Luo
authors.forename: Tai-Cheng
authors.initials: TC
authors.lastname: Bao
authors.forename: Chun-Yan
authors.initials: CY
authors.lastname: Li
authors.forename: Jiang
authors.initials: J
authors.lastname: Cheng
authors.forename: Xiao-Chun
authors.initials: XC
authors.lastname: Liu
keywords: IL-1β; TNF-α; arthritis; bizhongxiao decotion; methotrexate; rheumatoid