title: Enantioselective esterification of ibuprofen under microwave irradiation.
abstract: Enantioselective esterification of ibuprofen has been successfully carried out in an organic solvent catalyzed by recombinant APE 1547 (a thermophilic esterase from the archaeon Aeropyrum pernix K1). Here we used microwave irradiation (MW) as the mode of heating to improve the enzyme performance. Under the optimum conditions, the enzyme activity of APE 1547 was 4.16 μmol/mg/h and the enantioselectivity (E value) was 52.9. Compared with conventional heating, the enzyme activity and the enantioselectivity were increased about 21.9-fold and 1.4-fold, respectively. The results also indicated that APE 1547 can maintain 95% of its activity even after being used five times, suggesting that the enzyme is stable under low power MW conditions.
authors.affiliation: The Second Hospital of Jilin University, Changchun 130011, China.
authors.forename: Zecheng
authors.initials: Z
authors.lastname: Yang
authors.forename: Xuedun
authors.initials: X
authors.lastname: Niu
authors.forename: Xuedong
authors.initials: X
authors.lastname: Fang
authors.forename: Ge
authors.initials: G
authors.lastname: Chen
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Yue
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Dantong
authors.initials: D
authors.lastname: Zhao
authors.forename: Zhi
authors.initials: Z
authors.lastname: Wang
mesh_terms: D041522:Aeropyrum / Q000201:enzymology*; D019843:Archaeal Proteins / Q000737:chemistry*; D004950:Esterases / Q000737:chemistry*; D004951:Esterification; D007052:Ibuprofen / Q000737:chemistry*; D008872:Microwaves; D011994:Recombinant Proteins / Q000737:chemistry
chemical_list: D019843:Archaeal Proteins; D011994:Recombinant Proteins; D004950:Esterases; D007052:Ibuprofen