title: Sensitive analysis of aminoglycoside antibiotics via hyphenation of transient moving substitution boundary with field-enhanced sample injection in capillary electrophoresis.
abstract: A novel field-enhanced sample injection coupled with transient moving substitution boundary method in capillary electrophoresis was developed for aminoglycoside antibiotic (AG) analysis using 18-crown-6-tetracarboxylic acid (18C6H4) as a pseudostationary phase. Results indicated that the stacking mechanism of moving substitution boundary relied on the substitution reaction between 18C6H4-bonded AG complexes and Na(+) at the substitution boundary. The stacking mechanism as well as important parameters governing pre-concentration and separation have been investigated in order to obtain maximum resolution and sensitivity. Under optimized conditions, using a sample prepared in a low-conductivity matrix, the limits of detection for streptomycin, neomycin, and kanamycin were 0.62, 5.9 and 8.6 nM (S/N=3), respectively, and the detection sensitivities were improved 940-, 692-, and 415-fold, respectively. The method also gave accurate and reliable results in the analysis of AGs in river water samples.
authors.affiliation: Department of Chemistry, East China Normal University, 500 Dongchuan Road, Shanghai 200241, China.
authors.forename: Shuli
authors.initials: S
authors.lastname: Ge
authors.forename: Wanrong
authors.initials: W
authors.lastname: Tang
authors.forename: Ruobing
authors.initials: R
authors.lastname: Han
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhu
authors.forename: Qingjiang
authors.initials: Q
authors.lastname: Wang
authors.forename: Pingang
authors.initials: P
authors.lastname: He
authors.forename: Yuzhi
authors.initials: Y
authors.lastname: Fang
mesh_terms: D000617:Aminoglycosides / Q000032:analysis*; D000900:Anti-Bacterial Agents / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D045483:Rivers / Q000737:chemistry; D012680:Sensitivity and Specificity; D014874:Water Pollutants, Chemical / Q000032:analysis
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D014874:Water Pollutants, Chemical