title: Concordant mast cell and basophil production by individual hematopoietic blast colony-forming cells.
abstract: Previous studies have shown that mouse bone marrow cells can produce mast cells when stimulated in vitro by stem cell factor (SCF) and interleukin-3 (IL-3). Experiments to define the marrow cells able to generate mast cells showed that the most active subpopulations were the Kit(+) Sca1(-) progenitor cell fraction and the more ancestral Kit(+) Sca1(+) blast colony-forming cell fraction. In clonal cultures, up to 64% of blast colony-forming cells were able to generate mast cells when stimulated by SCF and IL-3, and, of these, the most active were those in the CD34(-) Flt3R(-) long-term repopulating cell fraction. Basophils, identified by the monoclonal antibody mMCP-8 to mouse mast cell serine protease-8, were also produced by 50% of blast colony-forming cells with a strong concordance in the production of both cell types by individual blast colony-forming cells. Enriched populations of marrow-derived basophils were shown to generate variable numbers of mast cells after a further incubation with SCF and IL-3. The data extend the repertoire of lineage-committed cells able to be produced by multipotential hematopoietic blast colony-forming cells and show that basophils and mast cells can have common ancestral cells and that basophils can probably generate mast cells at least under defined in vitro conditions.
authors.affiliation: Cancer and Haematology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville 3052, Victoria, Australia. metcalf@wehi.edu.au
authors.forename: Donald
authors.initials: D
authors.lastname: Metcalf
authors.forename: Ashley P
authors.initials: AP
authors.lastname: Ng
authors.forename: Tracey M
authors.initials: TM
authors.lastname: Baldwin
authors.forename: Ladina
authors.initials: L
authors.lastname: Di Rago
authors.forename: Sandra
authors.initials: S
authors.lastname: Mifsud
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D001399:Azure Stains; D001491:Basophils / Q000166:cytology*; D002454:Cell Differentiation / Q000502:physiology*; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D066298:In Vitro Techniques; D008407:Mast Cells / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D019089:Stem Cell Factor
chemical_list: D000911:Antibodies, Monoclonal; D001399:Azure Stains; D019089:Stem Cell Factor