title: High-throughput STR analysis for DNA database using direct PCR.
abstract: Since the Korean criminal DNA database was launched in 2010, we have focused on establishing an automated DNA database profiling system that analyzes short tandem repeat loci in a high-throughput and cost-effective manner. We established a DNA database profiling system without DNA purification using a direct PCR buffer system. The quality of direct PCR procedures was compared with that of conventional PCR system under their respective optimized conditions. The results revealed not only perfect concordance but also an excellent PCR success rate, good electropherogram quality, and an optimal intra/inter-loci peak height ratio. In particular, the proportion of DNA extraction required due to direct PCR failure could be minimized to <3%. In conclusion, the newly developed direct PCR system can be adopted for automated DNA database profiling systems to replace or supplement conventional PCR system in a time- and cost-saving manner.
authors.affiliation: DNA Analysis Laboratory, Division of Forensic DNA, Supreme Prosecutors' Office, Seoul, 137-730, Korea.
authors.forename: Jeong Eun
authors.initials: JE
authors.lastname: Sim
authors.forename: Su Jeong
authors.initials: SJ
authors.lastname: Park
authors.forename: Han Chul
authors.initials: HC
authors.lastname: Lee
authors.forename: Se-Yong
authors.initials: SY
authors.lastname: Kim
authors.forename: Jong Yeol
authors.initials: JY
authors.lastname: Kim
authors.forename: Seung Hwan
authors.initials: SH
authors.lastname: Lee
mesh_terms: D016172:DNA Fingerprinting / Q000379:methods*; D030561:Databases, Nucleic Acid; D006801:Humans; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction / Q000379:methods*
keywords: Korean DNA database; buccal FTA card; direct polymerase chain reaction; forensic science; high-throughput; offender; short tandem repeat