title: The role of lysyl oxidase-like 2 in the odontogenic differentiation of human dental pulp stem cells.
abstract: Adult human dental pulp stem cells (hDPSCs) are a unique population of precursor cells those are isolated from postnatal dental pulp and have the ability to differentiate into a variety of cell types utilized for the formation of a reparative dentin-like complex. Using LC-MS/MS proteomics approaches, we identified the proteins secreted from the differentiating hDPSCs in mineralization media. Lysyl oxidase-like 2 (LOXL2) was identified as a protein that was down-regulated in the hDPSCs that differentiate into odontoblast-like cells. The role of LOXL2 has not been studied in dental pulp stem cells. LOXL2 mRNA levels were reduced in differentiating hDPSCs, whereas the levels of other LOX family members including LOX, LOXL1, LOXL3, and LOXL4, are increased. The protein expression and secretion levels of LOXL2 were also decreased during odontogenic differentiation. Recombinant LOXL2 protein treatment to hDPSCs resulted in a dose-dependent decrease in the early differentiation and the mineralization accompanying with the lower levels of odontogenic markers such as DSPP, DMP-1 and ALP. These results suggest that LOXL2 has a negative effect on the differentiation of hDPSCs and blocking LOXL2 can promote the hDPSC differentiation to odontoblasts.
authors.affiliation: Department of Veterinary Biochemistry, Brain Korea 21 and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.
authors.forename: Joo-Hyun
authors.initials: JH
authors.lastname: Kim
authors.forename: Eun-Hyang
authors.initials: EH
authors.lastname: Lee
authors.forename: Hye-Jeong
authors.initials: HJ
authors.lastname: Park
authors.forename: Eui-Kyun
authors.initials: EK
authors.lastname: Park
authors.forename: Tae-Geon
authors.initials: TG
authors.lastname: Kwon
authors.forename: Hong-In
authors.initials: HI
authors.lastname: Shin
authors.forename: Je-Yoel
authors.initials: JY
authors.lastname: Cho
mesh_terms: D053687:Adult Stem Cells / Q000187:drug effects / Q000502:physiology*; D000594:Amino Acid Oxidoreductases / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D015415:Biomarkers / Q000378:metabolism; D002113:Calcification, Physiologic / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics*; D002478:Cells, Cultured; D054425:Chemokine CCL27 / Q000378:metabolism; D003782:Dental Pulp / Q000187:drug effects / Q000502:physiology*; D016326:Extracellular Matrix Proteins / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000235:genetics; D006801:Humans; D009805:Odontogenesis / Q000187:drug effects / Q000235:genetics*; D010750:Phosphoproteins / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D012795:Sialoglycoproteins / Q000378:metabolism; D055153:Stem Cell Niche; D000099311:Dentin Sialophosphoprotein
chemical_list: D000594:Amino Acid Oxidoreductases; D015415:Biomarkers; D054425:Chemokine CCL27; D016326:Extracellular Matrix Proteins; D010750:Phosphoproteins; D011994:Recombinant Proteins; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein; C492276:CCL27 protein, human; C513309:DMP1 protein, human; C119091:LOXL2 protein, human