title: [The effects of tissue inhibitor of metalloproteinases on beta-dystroglycan in salivary adenoid cystic carcinoma].
abstract: OBJECTIVE
  To investigate the expression of beta-dystroglycan (beta-DG) and the roles of tissue inhibitor of metalloproteinases (TIMPs) on beta-DG in salivary adenoid cystic carcinoma (SACC).
  
  
  METHODS
  beta-DG in highly lung metastatic cell line ACC-M and lowly lung metastatic one ACC-2 was tested by immunocytochemistry with different concentrations (10, 15, 20, 25 micromol x L(-1)) of TIMPs, and that without the regulation of TIMPs was served as controls. beta-DG was detected in seven specimens of SACC and ten cases of normal salivary gland tissues which were considered as a comparison group by immunohistochemistry.
  
  
  RESULTS
  There was no positive beta-DG immune-staining at the ACC-2 and ACC-M cell lines without TIMPs in the cell culture. beta-DG expressed after the regulation of TIMPs. beta-DG expression was localized predominantly in basement membrane of the acinus, while the negative results were distributed in the carcinoma cells and around the cancer cell nests.
  
  
  CONCLUSIONS
  Beta-DG is widely expressed by transmembrane protein that plays important roles in connecting the extracellular matrix to the cytoskeleton, the fracture of this structure means that it is easy to invade and transfer, so restoration of beta-DG expression by TIMPs is considered to be critical for successful treatment of SACC.
authors.affiliation: Postgraduate Education College, Ningxia Medical University, Yinchuan 750004, China.
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Hao
authors.forename: Jia
authors.initials: J
authors.lastname: Ma
authors.forename: Chenchen
authors.initials: C
authors.lastname: Si
authors.forename: Jia
authors.initials: J
authors.lastname: Xu
authors.forename: Jie
authors.initials: J
authors.lastname: Jing
mesh_terms: D003528:Carcinoma, Adenoid Cystic; D045744:Cell Line, Tumor; D049030:Dystroglycans; D006801:Humans; D007150:Immunohistochemistry; D012468:Salivary Gland Neoplasms; D019714:Tissue Inhibitor of Metalloproteinases
chemical_list: D019714:Tissue Inhibitor of Metalloproteinases; D049030:Dystroglycans