title: Integrins-FAK-Rho GTPases pathway in endothelial cells sense and response to surface wettability of plasma nanocoatings.
abstract: Vascular endothelial cell (EC) adhesion and migration are essential processes in re-endothelialization of implanted biomaterials, which are affected by surface properties of implanted materials such as surface wettability. Our previous study demonstrated that, as model substrates, EC adhesion/migration showed an opposite behavior on the hydrophobic and hydrophilic surfaces of plasma SiOx:H nanocoatings. Extending our previous works, the expression and distribution of crucial proteins in integrins-FAK-Rho GTPases signaling pathways were examined, respectively. The results showed that a hydrophilic surface could enhance the expression of focal adhesion protein associated with cell adhesion; however, the hydrophobic surface could improve the expression of Rho GTPases associated with cell migration and phosphorylation level of FAK, revealing the potential reason of surface wettability mediating different cells' adhesion/migration behaviors. These findings reveal the relationship and molecular mechanism of endothelial cell adhesion/migration, which was expected to guide the surface modification of implants for accelerating endothelialization.
authors.affiliation: Institute of Biomedical Engineering, School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Shen
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Ma
authors.forename: Min
authors.initials: M
authors.lastname: Gao
authors.forename: Yi
authors.initials: Y
authors.lastname: Lai
authors.forename: Guixue
authors.initials: G
authors.lastname: Wang
authors.forename: Qingsong
authors.initials: Q
authors.lastname: Yu
authors.forename: Fu-zhai
authors.initials: FZ
authors.lastname: Cui
authors.forename: Xiaoheng
authors.initials: X
authors.lastname: Liu
mesh_terms: D015153:Blotting, Western; D002448:Cell Adhesion / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000378:metabolism*; D042783:Endothelial Cells / Q000737:chemistry / Q000201:enzymology*; D005455:Fluorescent Antibody Technique; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000737:chemistry / Q000378:metabolism*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D006822:Hybrid Cells; D057927:Hydrophobic and Hydrophilic Interactions; D016023:Integrins / Q000737:chemistry / Q000378:metabolism*; D049329:Nanostructures / Q000737:chemistry*; D011474:Prosthesis Design; D015607:Stents; D013499:Surface Properties; D016608:Talin / Q000737:chemistry / Q000378:metabolism; D017155:Wettability; D020764:cdc42 GTP-Binding Protein / Q000737:chemistry / Q000378:metabolism; D020741:rho GTP-Binding Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D020099:Coated Materials, Biocompatible; D016023:Integrins; D016608:Talin; D051416:Focal Adhesion Protein-Tyrosine Kinases; D020764:cdc42 GTP-Binding Protein; D020741:rho GTP-Binding Proteins