title: 3T3-L1 adipocytes possess anandamide- and epinephrine-responsive machinery for MDM2 distribution to the plasma membrane.
abstract: The effects of biomolecules on peripheral tissues and their responsive machinery are not well understood. We examined MDM2 level in the plasma membrane (PM) and total MDM2 level of 3T3-L1 adipocytes treated with biomolecular anandamide, epinephrine, and other agents for 15 min. We also examined biomolecular responses in cells treated with mithramycin A, a binding inhibitor, or cells exposed to cooling and cell viability. Immunoblotting revealed that PM MDM2 level increased and total MDM2 level was not altered following treatment with anandamide, epinephrine, capsaicin, CL316243, and aluminum fluoride. PM MDM2 distribution caused by a biomolecular concentration was maintained by treatment with mithramycin A and exposure of cells to 28°C or 32°C but not to 18°C, and PM MDM2 levels after treatment with high concentrations of biomolecules were altered upon exposure to the inhibitor and mild hypothermia. These conditions did not decrease cell viability. Our findings indicate that 3T3-L1 adipocytes possess molecular machinery that responds differentially to anandamide and epinephrine under the inhibitor treatment and cool temperature conditions and that is sensitive to other agents (which mimic biomolecular responses); these machineries can induce subcellular alterations in molecular interactions. We provide information helpful for clarifying biomolecular responsive machinery present in 3T3-L1 adipocytes.
authors.affiliation: Department of Pharmacology, Chiba Institute of Science, Chiba, Japan. y-ohsaka@live.jp
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Ohsaka
authors.forename: Hoyoku
authors.initials: H
authors.lastname: Nishino
mesh_terms: D041721:3T3-L1 Cells; D000818:Animals; D001095:Arachidonic Acids / Q000008:administration & dosage*; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D063388:Endocannabinoids / Q000008:administration & dosage*; D004837:Epinephrine / Q000008:administration & dosage*; D051379:Mice; D053284:Polyunsaturated Alkamides / Q000008:administration & dosage*; D051736:Proto-Oncogene Proteins c-mdm2 / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D001095:Arachidonic Acids; D063388:Endocannabinoids; D053284:Polyunsaturated Alkamides; C497207:Mdm2 protein, mouse; D051736:Proto-Oncogene Proteins c-mdm2; C078814:anandamide; D004837:Epinephrine