title: Gene expression profiling of a compatible interaction between Douglas-Fir and the root rot fungal pathogen Phellinus sulphurascens.
abstract: Douglas-fir (DF) (Pseudotsuga menziesii) is one of the largest and most economically important coniferous species in western North America. Its productivity is greatly affected by the root rot fungus Phellinus sulphurascens Pilát. Evidence of resistance by DF to fungal root pathogens such as P. sulphurascens has been reported but mechanisms of resistance in this compatible pathosystem are not yet known. To better understand the DF-P. sulphurascens interaction, especially at the molecular level, we selected 12 diverse plant genes already identified as defense-related from a cDNA library constructed using root tissues from P. sulphurascens-infected DF seedlings. Using quantitative reverse-transcriptase polymerase chain reaction on infected DF root samples collected at five different time points after inoculation, we found that P. sulphurascens infection significantly elevated expression of the 12 selected genes. In most cases the highest expression level was recorded within 2 to 3 days after inoculation. The constructed cDNA library, which is enriched with defense-related host genes and a number of fungal genes, will continue to serve as a useful resource for future larger-scale gene discovery and functional research on the P. sulphurascens and DF pathosystem.
authors.affiliation: Natural Resources Canada, Canadian Forest Service, Pacific Forestry Centre, 506 West Burnside Road, Victoria, BC, V8Z 1M5, Canada.
authors.forename: M A
authors.initials: MA
authors.lastname: Islam
authors.forename: Rona N
authors.initials: RN
authors.lastname: Sturrock
authors.forename: Abul K M
authors.initials: AK
authors.lastname: Ekramoddoullah
mesh_terms: D001487:Basidiomycota / Q000502:physiology*; D018744:DNA, Plant / Q000235:genetics; D018506:Gene Expression Regulation, Plant / Q000276:immunology*; D015723:Gene Library; D010935:Plant Diseases / Q000382:microbiology*; D018517:Plant Roots / Q000382:microbiology*; D028224:Pseudotsuga / Q000382:microbiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D036226:Seedlings; D059467:Transcriptome
chemical_list: D018744:DNA, Plant