title: Effects of pomegranate seed oil on glucose and lipid metabolism-related organs in rats fed an obesogenic diet.
abstract: Studies conducted in mice have revealed positive effects of punicic acid (PUA). The aim of this study was to analyze the effects of PUA on fat accumulation and glycemic control in rats fed an obesogenic diet. Rats were randomly divided into two groups: control group and PUA group (diet supplemented with 0.5% PUA). No changes were observed in adipose tissue weights. The glucose tolerance test showed that the glycemic value in the PUA group had decreased significantly at the final time (120 min) (-19.3%), as had fructosamine levels (-11.1%). However, homeostasis model assessment (HOMA-IR) showed that insulin resistance did not improve. No changes were observed in the liver, skeletal muscle composition, or peroxisome proliferator-activated receptors (PPARs) activation. Low levels (mg/g tissue) of PUA (0.04 ± 0.01 in both tissues) and higher levels of cis-9,trans-11 conjugated linoleic acid (0.31 ± 0.08 in liver, 0.52 ± 0.11 in muscle) were found. PUA supplementation induced hypoplasia (-16.1%) due to the antiproliferative effect on hepatocytes. In conclusion, dietary supplementation of 0.5% PUA did not lead to decreased fat accumulation in adipose tissue, liver, or skeletal muscle, or to improved glycemic control. The hypoplasia induced in liver is a negative effect that should be considered before proposing PUA as a functional ingredient.
authors.affiliation: Nutrition and Obesity Group, Department of Nutrition and Food Science, Faculty of Pharmacy, University of the Basque Country (UPV/EHU), Paseo de la Universidad , 7. 01006 Vitoria, Spain.
authors.forename: Jonatan
authors.initials: J
authors.lastname: Miranda
authors.forename: Leixuri
authors.initials: L
authors.lastname: Aguirre
authors.forename: Alfredo
authors.initials: A
authors.lastname: Fernández-Quintela
authors.forename: M Teresa
authors.initials: MT
authors.lastname: Macarulla
authors.forename: M Guadalupe
authors.initials: MG
authors.lastname: Martínez-Castaño
authors.forename: Josune
authors.initials: J
authors.lastname: Ayo
authors.forename: Elisabette
authors.initials: E
authors.lastname: Bilbao
authors.forename: María P
authors.initials: MP
authors.lastname: Portillo
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D059305:Diet, High-Fat / Q000009:adverse effects*; D005951:Glucose Tolerance Test; D006801:Humans; D050356:Lipid Metabolism / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D029561:Lythraceae / Q000737:chemistry*; D008297:Male; D051379:Mice; D018482:Muscle, Skeletal / Q000187:drug effects / Q000378:metabolism; D009765:Obesity / Q000188:drug therapy* / Q000378:metabolism; D010938:Plant Oils / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds / Q000737:chemistry*
chemical_list: D001786:Blood Glucose; D010938:Plant Oils