title: An arabino(glucurono)xylan isolated from immunomodulatory active hemicellulose fraction of Salvia officinalis L.
abstract: From the aerial parts of sage (Salvia officinalis L.) an arabino-(4-O-methyl-glucurono)-xylan (AGX) was isolated by alkaline extraction followed by precipitation with barium hydroxide solution. Polymer was isolated from sage as a light brown polysaccharide material of molecular mass (Mp) 84,000. Compositional analyses of sage AGX revealed xylose (81%), arabinose (10%), glucuronic acid (8%) and small amounts of hexoses (1%). Linkage sugar analyses showed the (1→4)-linked xylopyranosyl backbone with low degree of substitution (9-10%) at O-2 and O-3. Arabinofuranose residues were found as the terminal, 1,3-, 1,5- and 1,3,5-linked. NMR structural analyses of acidic oligomers, generated by partial acidic hydrolysis of AGX, confirmed a substitution of xylose residues by glucuronic acid and its 4-O-methyl derivate at O-2 at an average on every fourteenth xylose residue. NMR and FT-IR measurements, as well as a high negative optical rotation confirmed the β configuration of glycosidic linkages in AGX backbone.
authors.affiliation: Institute of Chemistry, Center for Glycomics, Slovak Academy of Sciences, Dúbravská cesta 9, 845 38 Bratislava, Slovak Republic. Peter.Capek@savba.sk
authors.forename: P
authors.initials: P
authors.lastname: Capek
authors.forename: M
authors.initials: M
authors.lastname: Matulová
mesh_terms: D001089:Arabinose / Q000031:analogs & derivatives / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D007155:Immunologic Factors / Q000737:chemistry* / Q000302:isolation & purification; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D008970:Molecular Weight; D010429:Pentoses / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry*; D027543:Salvia officinalis / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D014990:Xylans / Q000737:chemistry* / Q000302:isolation & purification; D014994:Xylose / Q000737:chemistry
chemical_list: D007155:Immunologic Factors; D010429:Pentoses; D011134:Polysaccharides; D014990:Xylans; C431714:arabinofuranose; C114470:glucuronoarabinoxylan; C007916:hemicellulose; D020723:Glucuronic Acid; D014994:Xylose; D001089:Arabinose