title: MR detection of LPS-induced neutrophil activation using mannan-coated superparamagnetic iron oxide nanoparticles.
abstract: OBJECTIVE
  The purpose of this study was to investigate the potential of the phagocytic activity of lipopolysaccharide (LPS) induced neutrophils that are mannan coated with superparamagnetic iron oxide nanoparticles (M-SPION).
  
  
  METHODS
  Human neutrophils were divided into control and LPS groups. The neutrophils were labeled with M-SPION and dextran-coated SPION. After labeling of M-SPION, the mean signal intensity (SI) of the LPS group was significantly lower than that of the control group.
  
  
  RESULTS
  The labeling of both control and LPS groups with M-SPION showed significantly lower SI than those labeled with D-SPION. After labeling with M-SPION, the intracellular iron uptake of neutrophil in Prussian blue staining was markedly demonstrated in the LPS group, but not in the control group. M-SPION was more effective than D-SPION in the labeling of neutrophils in vitro.
  
  
  CONCLUSIONS
  The in vitro labeling technique of LPS neutrophil with M-SPION on MR imaging could be developed into a diagnostic method of LPS-induced neutrophils.
authors.affiliation: Department of Radiology, Chonnam National University Hwasun Hospital, Chonnam National University Medical School, Gwangju, 501-757, South Korea.
authors.forename: Han
authors.initials: H
authors.lastname: Shanhua
authors.forename: Han
authors.initials: H
authors.lastname: Huijing
authors.forename: Myeong Ju
authors.initials: MJ
authors.lastname: Moon
authors.forename: Suk Hee
authors.initials: SH
authors.lastname: Heo
authors.forename: Hyo Soon
authors.initials: HS
authors.lastname: Lim
authors.forename: In-Kyu
authors.initials: IK
authors.lastname: Park
authors.forename: Chong-Su
authors.initials: CS
authors.lastname: Cho
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Kwak
authors.forename: Yong Yeon
authors.initials: YY
authors.lastname: Jeong
mesh_terms: D002478:Cells, Cultured; D006801:Humans; D042541:Intracellular Space / Q000737:chemistry / Q000378:metabolism; D037181:Lectins, C-Type / Q000737:chemistry / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles / Q000032:analysis / Q000737:chemistry*; D008351:Mannans / Q000378:metabolism*; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins / Q000737:chemistry / Q000378:metabolism; D018375:Neutrophil Activation / Q000187:drug effects / Q000502:physiology*; D009504:Neutrophils / Q000737:chemistry* / Q000166:cytology* / Q000378:metabolism; D011956:Receptors, Cell Surface / Q000737:chemistry / Q000378:metabolism
chemical_list: D037181:Lectins, C-Type; D008070:Lipopolysaccharides; D058185:Magnetite Nanoparticles; D008351:Mannans; D000090323:Mannose Receptor; D037241:Mannose-Binding Lectins; D011956:Receptors, Cell Surface