title: Long-term stability of human genomic and human papillomavirus DNA stored in BD SurePath and Hologic PreservCyt liquid-based cytology media.
abstract: We evaluated the effect of storage at 2 to 8°C on the stability of human genomic and human papillomavirus (HPV) DNA stored in BD SurePath and Hologic PreservCyt liquid-based cytology media. DNA retained the ability to be extracted and PCR amplified for more than 2.5 years in both medium types. Prior inability to detect DNA in archived specimens may have been due to failure of the extraction method to isolate DNA from fixed cells.
authors.affiliation: BD Diagnostics, Sparks, Maryland, USA.
authors.forename: Patricia M
authors.initials: PM
authors.lastname: Agreda
authors.forename: Gerard H
authors.initials: GH
authors.lastname: Beitman
authors.forename: Erin C
authors.initials: EC
authors.lastname: Gutierrez
authors.forename: James M
authors.initials: JM
authors.lastname: Harris
authors.forename: Kristopher R
authors.initials: KR
authors.lastname: Koch
authors.forename: William D
authors.initials: WD
authors.lastname: LaViers
authors.forename: Sharon V
authors.initials: SV
authors.lastname: Leitch
authors.forename: Courtney E
authors.initials: CE
authors.lastname: Maus
authors.forename: Ray A
authors.initials: RA
authors.lastname: McMillian
authors.forename: William A
authors.initials: WA
authors.lastname: Nussbaumer
authors.forename: Marcus L R
authors.initials: ML
authors.lastname: Palmer
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Porter
authors.forename: Gregory A
authors.initials: GA
authors.lastname: Richart
authors.forename: Ryan J
authors.initials: RJ
authors.lastname: Schwab
authors.forename: Laurence M
authors.initials: LM
authors.lastname: Vaughan
mesh_terms: D003470:Culture Media / Q000737:chemistry; D003584:Cytological Techniques / Q000379:methods*; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D012034:Refrigeration; D013048:Specimen Handling / Q000379:methods*; D013997:Time Factors; D014773:Virology / Q000379:methods*
chemical_list: D003470:Culture Media; D004279:DNA, Viral