title: Variation of the effect of calcium phosphate enhancement of implanted silk fibroin ligament bone integration.
abstract: In this article, low crystallinity hydroxyapatite (LHA) is developed and utilized to modify silk fibroin scaffolds which are applied to repair bone/ligament defects successfully. It can promote osteogenesis which is authenticated through in vitro and in vivo tests. The scaffold is an efficient carrier, supporting cell proliferation and differentiation. Meanwhile, cytocompatibility and osteoblastic gene expressions (RUNX2 and osteocalcin, for example) of rabbit's bone marrow derived mesenchymal stem cells (MSCs) are significantly boosted on LHA/silk scaffold. Further, for animal trial, almost 60% of bone volume and 80% of original mechanical strength are recovered after 4 months' bone/ligament regeneration in bone tunnel of rabbit model, where significant amount of bone tissue regeneration is also confirmed by data of histological evaluation and micro computed tomography (μ-CT). Hence, the invented scaffold is applicable for ligament/bone regeneration in future lager animal and clinical trials.
authors.affiliation: Department of Bioengineering, National University of Singapore, Singapore.
authors.forename: Pujiang
authors.initials: P
authors.lastname: Shi
authors.forename: Thomas K H
authors.initials: TK
authors.lastname: Teh
authors.forename: Siew L
authors.initials: SL
authors.lastname: Toh
authors.forename: James C H
authors.initials: JC
authors.lastname: Goh
mesh_terms: D000818:Animals; D016118:Anterior Cruciate Ligament / Q000000981:diagnostic imaging / Q000187:drug effects / Q000502:physiology*; D001861:Bone Regeneration / Q000187:drug effects; D001842:Bone and Bones / Q000000981:diagnostic imaging / Q000187:drug effects / Q000502:physiology*; D002118:Calcium / Q000378:metabolism; D002130:Calcium Phosphates / Q000494:pharmacology*; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D003460:Crystallization; D017886:Durapatite / Q000494:pharmacology; D005349:Fibroins; D005786:Gene Expression Regulation / Q000187:drug effects; D020007:Implants, Experimental; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000648:ultrastructure; D016348:Osseointegration / Q000502:physiology*; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D015676:Osteonectin / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D011859:Radiography; D013194:Staining and Labeling; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D002130:Calcium Phosphates; D024042:Collagen Type I; D050659:Core Binding Factor Alpha 1 Subunit; D015676:Osteonectin; D015675:Osteocalcin; D005349:Fibroins; D017886:Durapatite; C020243:calcium phosphate; D002118:Calcium