title: Depolarizing actions of hydrogen sulfide on hypothalamic paraventricular nucleus neurons.
abstract: Hydrogen sulfide (H2S) is a novel neurotransmitter that has been shown to influence cardiovascular functions as well and corticotrophin hormone (CRH) secretion. Since the paraventricular nucleus of the hypothalamus (PVN) is a central relay center for autonomic and endocrine functions, we sought to investigate the effects of H2S on the neuronal population of the PVN. Whole cell current clamp recordings were acquired from the PVN neurons and sodium hydrosulfide hydrate (NaHS) was bath applied at various concentrations (0.1, 1, 10, and 50 mM). NaHS (1, 10, and 50 mM) elicited a concentration-response relationship from the majority of recorded neurons, with almost exclusively depolarizing effects following administration. Cells responded and recovered from NaHS administration quickly and the effects were repeatable. Input differences from baseline and during the NaHS-induced depolarization uncovered a biphasic response, implicating both a potassium and non-selective cation conductance. The results from the neuronal population of the PVN shed light on the possible physiological role that H2S has in autonomic and endocrine function.
authors.affiliation: Department of Biomedical and Molecular Science, Queen's University, Kingston, Ontario, Canada.
authors.forename: C Sahara
authors.initials: CS
authors.lastname: Khademullah
authors.forename: Alastair V
authors.initials: AV
authors.lastname: Ferguson
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D008297:Male; D009467:Neuromuscular Depolarizing Agents / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects*; D018377:Neurotransmitter Agents / Q000494:pharmacology*; D010286:Paraventricular Hypothalamic Nucleus / Q000166:cytology* / Q000187:drug effects; D018408:Patch-Clamp Techniques / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D013440:Sulfides / Q000494:pharmacology*
chemical_list: D009467:Neuromuscular Depolarizing Agents; D018377:Neurotransmitter Agents; D013440:Sulfides; C025451:sodium bisulfide