title: Protective effect of a Butea monosperma (Lam.) Taub. flowers extract against skin inflammation: antioxidant, anti-inflammatory and matrix metalloproteinases inhibitory activities.
abstract: BACKGROUND
  Butea monosperma (Lam.) Taubert (Syn. Butea frondosa; family Fabaceae) is a common plant of the Indian continent (Das et al., 2011; Sharma and Deshwal, 2011). The brightly orange flowers of this plant are widely used in traditional medicine and more particularly for inflammatory disease.
  
  
  OBJECTIVE
  In vitro anti-inflammatory mechanism of a hydroethanolic extract of B. monosperma flowers (BME) and more specifically of an enriched fraction in butrin and isobutrin (BI) was studied using cell culture of Normal Human Keratinocyte, cells involved in the skin inflammatory.
  
  
  METHODS
  Dried and crushed B. monosperma flowers were extracted with Ethanol/H2O (70/30 v/v). The butrin/isobutrin fraction was obtained by centrifugal Partition Chromatography (CPC). Experiments were conducted on UV-B treated normal human epidermis keratinocytes, cells involved in the skin inflammatory response. To evaluate extract anti-inflammatory activity, cytokines IL-1β, IL-6, IL-8, prostaglandin E2 and metalloproteinases MMP-1, -2, -9 and -10 were measured in the cells supernatant.
  
  
  RESULTS
  Our data clearly showed that hydroalcoholic B. monosperma flower extract was able to decrease the secretion of IL-1β, IL-6 and IL-8 pro-inflammatory cytokines of -32, -33 and -18% respectively. Interestingly, Prostaglandin E2 production and the secretion of MMP-1, -2, -9 and -10 were also inhibited. Same results were observed in presence of enriched fraction in butrin and isobutrin and confirmed the participation of these molecules in the anti-inflammatory activity.
  
  
  CONCLUSIONS
  These results explain the anti-inflammatory activity of B. monosperma and confirm the interest to use it in traditional Indian medicine. Moreover, its metalloproteinases inhibitory activities coupled with its anti-inflammatory action also give anti-aging property to this plant.
authors.affiliation: LVMH Recherche, BP58, 45800 Saint Jean de Braye, France. irenimel@research.lvmh-pc.com
authors.forename: Isabelle
authors.initials: I
authors.lastname: Krolikiewicz-Renimel
authors.forename: Thomas
authors.initials: T
authors.lastname: Michel
authors.forename: Emilie
authors.initials: E
authors.lastname: Destandau
authors.forename: Madhukar
authors.initials: M
authors.lastname: Reddy
authors.forename: Patrice
authors.initials: P
authors.lastname: André
authors.forename: Claire
authors.initials: C
authors.lastname: Elfakir
authors.forename: Chantal
authors.initials: C
authors.lastname: Pichon
mesh_terms: D000328:Adult; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D031290:Butea / Q000737:chemistry*; D002478:Cells, Cultured; D047188:Chalcones / Q000737:chemistry / Q000494:pharmacology; D016207:Cytokines / Q000378:metabolism; D003872:Dermatitis / Q000188:drug therapy* / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D004817:Epidermis / Q000187:drug effects / Q000378:metabolism; D005260:Female; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology; D035264:Flowers / Q000737:chemistry*; D006801:Humans; D007249:Inflammation / Q000188:drug therapy / Q000378:metabolism; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D012867:Skin / Q000187:drug effects / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D047188:Chalcones; D016207:Cytokines; D005419:Flavonoids; D010936:Plant Extracts; C049386:butrin; C049385:isobutrin; D020782:Matrix Metalloproteinases; D015232:Dinoprostone