title: Functional nanofiber mat of polyvinyl alcohol/gelatin containing nanoparticles of biphasic calcium phosphate for bone regeneration in rat calvaria defects.
abstract: New biodegradable mats was successfully obtained by functional polyvinyl alcohol (PVA)/Gelatin (GE) blend fiber mats containing different BCP amounts (20, 40, and 50 w/v%) of biphasic calcium phosphate (BCP) nanoparticles for bone regeneration. BCP nanoparticles were loaded and dispersed successfully in the PVA/GE fibrous matrix. The addition of BCP was found to have increased fiber diameter, tensile strength, osteoblast cell adhesion, proliferation, and protein expression. Compared to the others, the 50% BCP-loaded electrospun PVA/GE fibers had the most favorable mechanical properties, cell attachment and growth, and protein expression. In vivo bone formation was examined using rat models, and increased bone formation was observed for the 50% BCP-loaded electrospun PVA/GE blends within 2 and 4 weeks. This result suggests that the 50% BCP-PVA/GE composite nanofiber mat has high potential for use in the field of bone regeneration and tissue engineering.
authors.affiliation: Department of Biomedical Engineering & Material, College of Medicine, Soonchunhyang University, 366-1 Ssangyong dong, Cheonan, South Korea.
authors.forename: Nguyen Thuy Ba
authors.initials: NT
authors.lastname: Linh
authors.forename: Kap-Ho
authors.initials: KH
authors.lastname: Lee
authors.forename: Byong-Taek
authors.initials: BT
authors.lastname: Lee
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D018786:Bone Substitutes / Q000737:chemistry*; D002460:Cell Line; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D006882:Hydroxyapatites / Q000737:chemistry*; D008297:Male; D008422:Materials Testing; D057139:Nanofibers / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry; D010006:Osteoblasts / Q000166:cytology; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012886:Skull / Q000293:injuries / Q000502:physiology*
chemical_list: D018786:Bone Substitutes; D006882:Hydroxyapatites; C074950:hydroxyapatite-beta tricalcium phosphate; D005780:Gelatin; D011142:Polyvinyl Alcohol