title: Expression of group IIA phospholipase A2 is an independent predictor of favorable outcome for patients with gastric cancer.
abstract: Growing evidence suggests that phospholipase A2 (PLA2) plays a pivotal role in tumorigenesis in human gastrointestinal cancer. One of the well-studied isoforms of PLA2, group IIA PLA2 (PLA2G2A), appears to exert its protumorigenic or antitumorigenic effects in a tissue-specific manner. The present study was designed to determine the expression profile and prognostic value of PLA2G2A in gastric cancer in a large Chinese cohort. By using real-time polymerase chain reaction, the amount of PLA2G2A messenger RNA in 60 pairs of fresh gastric tumors and adjacent noncancerous mucosa was measured. The immunostaining of PLA2G2A in 866 gastric cancers with paired noncancerous tissues was assayed. No expression of PLA2G2A was found in normal gastric mucosa, and focal expression of PLA2G2A was noticed in intestinal metaplasia, whereas significantly increased expression of PLA2G2A was observed in the cytoplasm of gastric cancer cells. Furthermore, the extent of PLA2G2A expression was associated with tumor size (P < .001), tumor differentiation (P = .001), T class (P < .001), N class (P < .001), and TNM stage (P < .001) of gastric cancer. Multivariate analysis showed that PLA2G2A expression was an independent predictor of survival for patients with gastric cancer (P = .024). Expression of PLA2G2A seems to be protective for patients with gastric cancer (hazard ratio, 1.423; 95% confidence interval, 1.047-1.935), and it may be a target for achieving better treatment outcomes.
authors.affiliation: Department of Oncology, Changzheng Hospital, Second Military Medical University, Shanghai 200070, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Wang
authors.forename: Chun-Jin
authors.initials: CJ
authors.lastname: Huang
authors.forename: Guan-Zhen
authors.initials: GZ
authors.lastname: Yu
authors.forename: Jie-Jun
authors.initials: JJ
authors.lastname: Wang
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
authors.forename: Yu-Mei
authors.initials: YM
authors.lastname: Li
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology* / Q000235:genetics / Q000401:mortality / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002681:China / Q000453:epidemiology; D003593:Cytoplasm / Q000201:enzymology / Q000473:pathology; D005260:Female; D005753:Gastric Mucosa / Q000201:enzymology; D015870:Gene Expression; D020869:Gene Expression Profiling; D054501:Group II Phospholipases A2 / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008297:Male; D008679:Metaplasia; D008875:Middle Aged; D009367:Neoplasm Staging; D011230:Precancerous Conditions / Q000201:enzymology / Q000235:genetics / Q000473:pathology; D011379:Prognosis; D012333:RNA, Messenger / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D013270:Stomach / Q000201:enzymology / Q000473:pathology; D013274:Stomach Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000401:mortality / Q000473:pathology; D015996:Survival Rate; D055815:Young Adult
chemical_list: D012333:RNA, Messenger; D054501:Group II Phospholipases A2; C516287:PLA2G2A protein, human
keywords: Gastric cancer; Group IIA phospholipase A2; Immunohistochemistry; Prognosis; Tissue microarray