title: Transcriptional wiring for establishing cell lineage specification at the blastocyst stage in cattle.
abstract: Mice and cattle use distinct pathways for the first cell segregation into inner cell mass (ICM) and trophectoderm (TE) lineages at the blastocyst stage. However, limited knowledge is available regarding the reliable transcriptional networks that orchestrate the complex developmental processes at this stage in nonrodent species. In order to elucidate the site-dominant transcriptomic properties of bovine blastocysts, we separated cell samples into the ICM and TE using both mechanical and chemical methods and performed in silico prescreening for candidate genes that were site-dominantly expressed in bovine blastocysts. We further performed quantitative real-time PCR and in situ hybridization using the site-specific cell samples. As a result, we identified seven ICM-dominant genes and five TE-dominant genes not found in earlier studies. Our findings provide novel insights into the mechanism of cell-fate specification in the pre-implantation bovine embryo.
authors.affiliation: Laboratory of Animal Breeding and Reproduction, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Nagatomo
authors.forename: Shinjiro
authors.initials: S
authors.lastname: Kagawa
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Kishi
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Takuma
authors.forename: Ayari
authors.initials: A
authors.lastname: Sada
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Yamanaka
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Abe
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Wada
authors.forename: Masashi
authors.initials: M
authors.lastname: Takahashi
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Kono
authors.forename: Manabu
authors.initials: M
authors.lastname: Kawahara
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000166:cytology* / Q000378:metabolism; D053624:Blastocyst Inner Cell Mass / Q000378:metabolism; D002417:Cattle; D002454:Cell Differentiation / Q000235:genetics; D019070:Cell Lineage / Q000235:genetics*; D046149:Embryo Culture Techniques; D047108:Embryonic Development / Q000235:genetics*; D005260:Female; D005307:Fertilization in Vitro; D018507:Gene Expression Regulation, Developmental; D053263:Gene Regulatory Networks
keywords: blastocyst; bovine; cell lineage specification; gene expression