title: Opposing functions of PLAG1 in pleomorphic adenoma: a microarray analysis of PLAG1 transgenic mice.
abstract: Over-expression of the proto-oncogene pleomorphic adenoma gene 1 (PLAG1) plays a crucial role in the formation of pleomorphic adenoma, which is the most common type of salivary gland tumor. To understand the molecular mechanisms governing PLAG1-mediated tumorigenesis, we used a microarray-based approach to identify PLAG1 target genes. We validated the expression of several genes, including Bax, Fas, p53, p21, p16, Cyclin D1, Egfr, Trail-R/DR5, c-Fos, c-myc and Igf2, by real-time RT-PCR or western blotting. Using luciferase reporter gene assays, we determined that the promoters of Bax, Fas, p53, TRAIL-R/DR5, and c-Fos were transactivated by PLAG1. PLAG1 not only activates genes that promote cell proliferation and tumor formation but also genes that inhibit these cellular processes. Therefore, we conclude that PLAG1 may play a dual role in tumor formation.
authors.affiliation: Department of Oral and Maxillofacial Surgery, College of Stomatology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Wenjun
authors.initials: W
authors.lastname: Shang
authors.forename: Xia
authors.initials: X
authors.lastname: Lei
authors.forename: Shukun
authors.initials: S
authors.lastname: Shen
authors.forename: Hongxin
authors.initials: H
authors.lastname: Zhang
authors.forename: Zhugang
authors.initials: Z
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Huang
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Yu
authors.forename: Huishan
authors.initials: H
authors.lastname: Ong
authors.forename: Xuelai
authors.initials: X
authors.lastname: Yin
authors.forename: Wenjun
authors.initials: W
authors.lastname: Yang
authors.forename: Chenping
authors.initials: C
authors.lastname: Zhang
mesh_terms: D008949:Adenoma, Pleomorphic / Q000235:genetics*; D000818:Animals; D019677:Artificial Gene Fusion; D015153:Blotting, Western; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D046228:Microarray Analysis; D000090063:Proto-Oncogene Mas; D060888:Real-Time Polymerase Chain Reaction; D015533:Transcriptional Activation
chemical_list: D004268:DNA-Binding Proteins; C000715470:MAS1 protein, human; C104382:PLAG1 protein, human; D000090063:Proto-Oncogene Mas; D008156:Luciferases