title: Liquid chromatographic determination of alogliptin in bulk and in its pharmaceutical preparation.
abstract: In this work, a reversed-phase liquid chromatographic (RP-LC) method has been developed for the determination of alogliptin (ALG) based on isocratic elution using a mobile phase consisting of potassium dihydrogen phosphate buffer pH (4.6)-acetonitrile (20:80, v/v) at a flow rate of 1 mL min(-1) with UV detection at 215 nm. Chromatographic separation was achieved on a Symmetry(®) cyanide column (150 mm × 4.6 mm, 5 μm). Linearity, accuracy and precision were found to be acceptable over the concentration range of 5-160 μg mL(-1) for ALG in bulk. The optimized method was validated and proved to be specific, robust and accurate for the quality control of ALG in pharmaceutical preparations.
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Cairo University, Kasr El-Aini St., Cairo 11562, Egypt.
authors.forename: Ramzia I
authors.initials: RI
authors.lastname: El-Bagary
authors.forename: Ehab F
authors.initials: EF
authors.lastname: Elkady
authors.forename: Bassam M
authors.initials: BM
authors.lastname: Ayoub
keywords: alogliptin; isocratic elution; pharmaceutical preparation; reversed-phase liquid chromatography