title: The effect of increased centrifugation temperature on the quality of red-blood-cell concentrates of automated whole blood processing.
abstract: OBJECTIVE
  There are manual and automated methods to separate whole blood (WB) available. The Atreus whole blood processing system is an automated method, which combines centrifugation and expression of components into a single device. A major difference to conventional methods is that centrifugation temperature is not controlled at 22°C. The aim of this study was to examine the influence of increased centrifugation temperatures on the quality of red-blood-cell concentrates (RCC) after active cooling of WB prior to processing.
  
  
  METHODS
  A total of 28 WB were processed: 16 at centrifugation temperatures of up to 28°C (1st protocol) and 12 at 34°C (2nd protocol). RCC quality parameters were tested weekly for 42 days.
  
  
  RESULTS
  Red-blood-cell concentrates (RCC) quality complied with the European and German guidelines. Haemolysis was not significantly different throughout storage. Significant statistical differences were detected between both protocols in potassium concentration at the end of storage and in ATP levels at the day of processing.
  
  
  CONCLUSIONS
  Centrifugation temperatures of up to 34°C are well tolerated by the red blood cells with minimal interference with the RCC quality parameters.
authors.affiliation: Institute of Transfusion Medicine, University Hospital Jena, Jena, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Weinigel
authors.forename: S
authors.initials: S
authors.lastname: Rummler
authors.forename: D
authors.initials: D
authors.lastname: Barz
mesh_terms: D002469:Cell Separation / Q000379:methods*; D002498:Centrifugation / Q000379:methods; D004906:Erythrocyte Count; D004912:Erythrocytes / Q000737:chemistry* / Q000166:cytology* / Q000382:microbiology; D006461:Hemolysis; D006801:Humans; D010976:Platelet Count; D011188:Potassium / Q000032:analysis; D013696:Temperature
chemical_list: D011188:Potassium
keywords: blood processing; component production; red cell components