title: Effect of avian influenza A H5N1 infection on the expression of microRNA-141 in human respiratory epithelial cells.
abstract: BACKGROUND
  Avian influenza remains a serious threat to human health. The consequence of human infection varies markedly among different subtypes of avian influenza viruses. In addition to viral factors, the difference in host cellular response is likely to play a critical role. This study aims at elucidating how avian influenza infection perturbs the host's miRNA regulatory pathways that may lead to adverse pathological events, such as cytokine storm, using the miRNA microarray approach.
  
  
  RESULTS
  The results showed that dysregulation of miRNA expression was mainly observed in highly pathogenic avian influenza A H5N1 infection. We found that miR-21*, miR-100*, miR-141, miR-574-3p, miR-1274a and miR1274b were differentially expressed in response to influenza A virus infection. Interestingly, we demonstrated that miR-141, which was more highly induced by H5N1 than by H1N1 (p < 0.05), had an ability to suppress the expression of a cytokine - transforming growth factor (TGF)-β2. This was supported by the observation that the inhibitory effect could be reversed by antagomiR-141.
  
  
  CONCLUSIONS
  Since TGF-β2 is an important cytokine that can act as both an immunosuppressive agent and a potent proinflammatory molecule through its ability to attract and regulate inflammatory molecules, and previous report showed that only seasonal influenza H1N1 (but not the other avian influenza subtypes) could induce a persistent expression of TGF-β2, we speculate that the modulation of TGF-β2 expression by different influenza subtypes via miR-141 might be a critical step for determining the outcome of either normal or excessive inflammation progression.
authors.affiliation: School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, New Territories, Hong Kong Special Administration Region, Shatin, People's Republic of China.
authors.forename: Wai-Yip
authors.initials: WY
authors.lastname: Lam
authors.forename: Apple Chung-Man
authors.initials: AC
authors.lastname: Yeung
authors.forename: Karry Lei-Ka
authors.initials: KL
authors.lastname: Ngai
authors.forename: Man-Shan
authors.initials: MS
authors.lastname: Li
authors.forename: Ka-Fai
authors.initials: KF
authors.lastname: To
authors.forename: Stephen Kwok-Wing
authors.initials: SK
authors.lastname: Tsui
authors.forename: Paul Kay-Sheung
authors.initials: PK
authors.lastname: Chan
mesh_terms: D002460:Cell Line; D004847:Epithelial Cells / Q000821:virology*; D020869:Gene Expression Profiling; D054884:Host-Pathogen Interactions; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000472:pathogenicity*; D035683:MicroRNAs / Q000096:biosynthesis*
chemical_list: C533895:MIRN141 microRNA, human; D035683:MicroRNAs