title: Isolation and characterization of a turkey arthritis reovirus.
abstract: During the spring and summer of 2011, the Minnesota Veterinary Diagnostic Laboratory at the University of Minnesota received 14 submissions of 15-to-18-week-old tom turkeys that were recumbent with wing tip bruises ("wing walkers") and uni- or bilateral swelling of the hock (tibiotarsal) joints. Gastrocnemius or digital flexor tendons were occasionally ruptured. A total of five turkey arthritis reoviruses (TARV-MN1 through TARV-MN5) were isolated in specific-pathogen-free embryonated chicken eggs and QT-35 cells. The identity of the isolates was confirmed by electron microscopy, reverse transcription-polymerase chain reaction, and gene sequence analysis. BLAST analysis on the basis of a 880 bp nucleotide sequence of the S4 gene confirmed all isolates as a reovirus. Phylogenetic analysis divided the five isolates into two subgroups: subgroup I containing TARV-MN1, -2, -3, and -5, and the other subgroup containing TARV-MN4. Isolates in subgroup I had a similarity of 97%-100% with each other, while subgroup II (TARV-MN4) had a similarity of only 89.2% with subgroup I viruses. This isolate showed 90%-93% similarity with turkey enteric reoviruses in the United States, while the other four isolates in subgroup I had 89%-97.6% similarity. These results indicate divergence within TARVs as well as from enteric viruses, which needs to be confirmed by complete genome sequence analysis. Further experimental studies are planned to determine the role of these isolates in turkey arthritis and to compare them with classical chicken reovirus.
authors.affiliation: Department of Veterinary Population Medicine and Veterinary Diagnostic Laboratory, University of Minnesota, 1333 Gortner Avenue, St. Paul, MN 55108, USA.
authors.forename: Sunil K
authors.initials: SK
authors.lastname: Mor
authors.forename: Tamer A
authors.initials: TA
authors.lastname: Sharafeldin
authors.forename: Robert E
authors.initials: RE
authors.lastname: Porter
authors.forename: Andre
authors.initials: A
authors.lastname: Ziegler
authors.forename: Devi P
authors.initials: DP
authors.lastname: Patnayak
authors.forename: Sagar M
authors.initials: SM
authors.lastname: Goyal
mesh_terms: D000818:Animals; D007794:Lameness, Animal / Q000821:virology*; D008910:Minnesota; D008969:Molecular Sequence Data; D030101:Orthoreovirus, Avian / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D011201:Poultry Diseases / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D020539:Sequence Analysis, Protein / Q000662:veterinary; D017423:Sequence Analysis, RNA / Q000662:veterinary; D017385:Sequence Homology; D013717:Tenosynovitis / Q000662:veterinary* / Q000821:virology; D014422:Turkeys; D054334:Viral Regulatory and Accessory Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D054334:Viral Regulatory and Accessory Proteins; C031657:sigma NS protein, Reovirus