title: Antioxidant activity of selected stilbenoids and their bioproduction in hairy root cultures of muscadine grape (Vitis rotundifolia Michx.).
abstract: Stilbenoids are polyphenolic phytoalexins with health-related properties in humans. Muscadine grape ( Vitis rotundifolia ) hairy root cultures were established via Agrobacterium rhizogenes -mediated transformation, and the effects of growth regulators (3-indolebutyric acid and 6-benzylaminopurine) and methyl jasmonate (MeJA) on stilbenoid production were studied. Twenty-one-day-old hairy root cultures were treated with 100 μM MeJA for 24 h, and then the stilbenoids were extracted from the medium and tissue with ethyl acetate and analyzed by HPLC. Resveratrol, piceid, and ε-viniferin were observed preferentially in tissue, whereas piceatannol was observed only in medium. Growth regulators did not affect the yield of stilbenoids, whereas higher levels were found upon treatment with MeJA. Stilbenoids identified in the hairy root cultures were analyzed for their radical scavenging capacity showing piceatannol and ε-viniferin as the strongest antioxidants. Muscadine grape hairy root cultures were demonstrated to be amenable systems to study stilbenoid biosynthesis and a sustainable source of these bioactive compounds.
authors.affiliation: Arkansas Biosciences Institute and ‡Department of Biological Sciences, Arkansas State University, State University, Arkansas 72467, United States.
authors.forename: Cesar
authors.initials: C
authors.lastname: Nopo-Olazabal
authors.forename: John
authors.initials: J
authors.lastname: Hubstenberger
authors.forename: Luis
authors.initials: L
authors.lastname: Nopo-Olazabal
authors.forename: Fabricio
authors.initials: F
authors.lastname: Medina-Bolivar
mesh_terms: D000975:Antioxidants / Q000032:analysis* / Q000378:metabolism; D018929:Cell Culture Techniques; D002851:Chromatography, High Pressure Liquid; D010936:Plant Extracts / Q000032:analysis* / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism; D013267:Stilbenes / Q000032:analysis* / Q000378:metabolism; D027843:Vitis / Q000737:chemistry* / Q000254:growth & development / Q000378:metabolism
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D013267:Stilbenes