title: Mesenchymal stromal cell characteristics vary depending on their origin.
abstract: Mesenchymal stromal cells (MSCs) are rare progenitor cells that can be isolated from various tissues. They exhibit multilineage differentiation potential, support regenerative processes, and interact with various immune cells. Therefore, MSCs represent a promising tool for regenerative medicine. However, source-dependent and donor-dependent differences of MSC properties, including implications on their clinical application are still largely unknown. We evaluated MSCs derived from perinatal tissues umbilical cord (UC) and amniotic membrane (AM) in comparison to adult MSCs from bone marrow (BM), which were used as gold standard. We found genetic background-independent differences between MSCs from UC and AM. While AM- and UC-MSCs were closer to each other than to BM-MSCs, they also exhibited differences between each other. AM-MSCs from different donors but not UC-MSCs displayed high interdonor variability. In addition, we show that although all MSCs expressed similar surface markers, MSC populations from UC and AM showed differential profiles of gene expression and paracrine factor secretion to BM-derived MSCs. Notably, pathway analysis of gene expression data revealed intriguing differences between MSCs suggesting that MSCs from UC and AM possess in general a higher potential of immunomodulatory capacity, whereas BM-MSCs showed a higher potential of supporting regenerative processes as exemplified by neuronal differentiation and development. These differences between perinatal and BM-derived MSCs may be relevant for clinical applications.
authors.affiliation: 1 Roche Diagnostics GmbH , pharma Research and Early Development (pRED), Penzberg, Germany .
authors.forename: Heike
authors.initials: H
authors.lastname: Wegmeyer
authors.forename: Ann-Marie
authors.initials: AM
authors.lastname: Bröske
authors.forename: Mathias
authors.initials: M
authors.lastname: Leddin
authors.forename: Karin
authors.initials: K
authors.lastname: Kuentzer
authors.forename: Anna Katharina
authors.initials: AK
authors.lastname: Nisslbeck
authors.forename: Julia
authors.initials: J
authors.lastname: Hupfeld
authors.forename: Kornelius
authors.initials: K
authors.lastname: Wiechmann
authors.forename: Jennifer
authors.initials: J
authors.lastname: Kuhlen
authors.forename: Christoffer
authors.initials: C
authors.lastname: von Schwerin
authors.forename: Carsten
authors.initials: C
authors.lastname: Stein
authors.forename: Saskia
authors.initials: S
authors.lastname: Knothe
authors.forename: Jürgen
authors.initials: J
authors.lastname: Funk
authors.forename: Ralf
authors.initials: R
authors.lastname: Huss
authors.forename: Markus
authors.initials: M
authors.lastname: Neubauer
mesh_terms: D000650:Amnion / Q000166:cytology / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D019070:Cell Lineage; D049109:Cell Proliferation; D064987:Cell- and Tissue-Based Therapy; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D015870:Gene Expression; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D059785:Karyotype; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D010920:Placenta / Q000166:cytology*; D011247:Pregnancy; D012038:Regeneration; D014470:Umbilical Cord / Q000166:cytology / Q000378:metabolism
chemical_list: D016207:Cytokines; D036341:Intercellular Signaling Peptides and Proteins