title: Development and comparison of two dispersive liquid-liquid microextraction techniques coupled to high performance liquid chromatography for the rapid analysis of bisphenol A in edible oils.
abstract: In this study, two novel sample extraction methods for the analysis of bisphenol A (BPA) in edible oils were developed by using liquid-liquid extraction followed by a dispersive liquid-liquid microextraction (LLE-DLLME) and reversed-phase dispersive liquid-liquid microextraction (RP-DLLME). RP-DLLME showed a superior characteristic over LLE-DLLME and other previously reported procedures because of its easy operation, short extraction time, high sensitivity, low organic solvent consumption and waste generation. The optimized extraction conditions of RP-DLLME for 1.0 g of edible oil diluted in 4 mL of n-hexane were: extractant, 100 μL 0.2 M sodium hydroxide solution (80% methanol, v/v); extraction time, 1 min; centrifugation, 3 min. The determination of BPA was carried out by high performance liquid chromatography coupled with a DAD detector. The method offered excellent linearity over a range of 0.010-0.5 μg g(-1) with a correlation coefficient of r>0.997. Intra-day and inter-day repeatability values expressed as relative standard deviation were 1.9% and 5.9%, respectively. The quantitation limit and detection limit were 6.3 and 2.5 ng g(-1). The target analyte was detected in 5 out of 16 edible oil samples. The recovery rates in real samples ranged from 89.5 to 99.7%.
authors.affiliation: College of Science, Northwest A&F University, Yangling, China. shuhliu@nwsuaf.edu.cn
authors.forename: Shuhui
authors.initials: S
authors.lastname: Liu
authors.forename: Qilong
authors.initials: Q
authors.lastname: Xie
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
authors.forename: Janzhi
authors.initials: J
authors.lastname: Sun
authors.forename: Hui
authors.initials: H
authors.lastname: He
authors.forename: Xiaoke
authors.initials: X
authors.lastname: Zhang
mesh_terms: D001559:Benzhydryl Compounds / Q000032:analysis* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D005506:Food Contamination / Q000032:analysis*; D057230:Limit of Detection; D059627:Liquid Phase Microextraction / Q000379:methods*; D010636:Phenols / Q000032:analysis* / Q000302:isolation & purification*; D010938:Plant Oils / Q000032:analysis*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D010636:Phenols; D010938:Plant Oils; C006780:bisphenol A; D000098826:Bisphenol A Compounds