title: Rapid analysis of mono-saccharides and oligo-saccharides in hydrolysates of lignocellulosic biomass by HPLC.
abstract: HPLC using pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone (PMP) was used to analyse mono-saccharides and oligo-saccharides in hydrolysates of lignocellulosic biomass. PMP derivatives, including those of mannose, rhamnose, cellobiose, glucose, xylose and arabinose, were separated within 14 min with detection at 254 nm. The method was also suitable for xylo-oligosaccharides (XOS): PMP derivatives of xylohexaose, xylopentaose, xylotetraose, xylotriose and xylobiose were well separated under the same conditions. The method was used to determine the mono-saccharide composition of Miscanthus and evaluate the production of XOS from enzymatic hydrolysis of crude xylan.
authors.affiliation: School of Energy Research, Xiamen University, Xiamen, 361005, People's Republic of China.
authors.forename: Hailong
authors.initials: H
authors.lastname: Li
authors.forename: Chuannan
authors.initials: C
authors.lastname: Long
authors.forename: Juan
authors.initials: J
authors.lastname: Zhou
authors.forename: Jian
authors.initials: J
authors.lastname: Liu
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Wu
authors.forename: Minnan
authors.initials: M
authors.lastname: Long
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*; D009005:Monosaccharides / Q000032:analysis*; D009844:Oligosaccharides / Q000032:analysis*; D006109:Poaceae / Q000378:metabolism*
chemical_list: D009005:Monosaccharides; D009844:Oligosaccharides; C036909:lignocellulose; D008031:Lignin; D006026:Glycoside Hydrolases