title: Solid phase synthesis and biological evaluation of probestin as an angiogenesis inhibitor.
abstract: Probestin is a potent aminopeptidase N (APN) inhibitor originally isolated from the bacterial culture broth. Here, we report probestin synthesis by solid phase peptide synthesis (SPPS) method and evaluated its activity to inhibit angiogenesis using a chicken embryo chorioallantoic membrane (CAM) assay and a CAM tumor xenograft model. Results from these studies demonstrate that probestin inhibits the angiogenic activity and tumor growth.
authors.affiliation: Department of Pharmaceutical Sciences, College of Pharmacy, The University of Oklahoma Health Sciences Center, 1110 N. Stonewall Avenue, Room 301, Oklahoma City, OK 73117, USA.
authors.forename: Gopal
authors.initials: G
authors.lastname: Pathuri
authors.forename: Jessica E
authors.initials: JE
authors.lastname: Thorpe
authors.forename: Bryan C
authors.initials: BC
authors.lastname: Disch
authors.forename: Lora C
authors.initials: LC
authors.lastname: Bailey-Downs
authors.forename: Michael A
authors.initials: MA
authors.lastname: Ihnat
authors.forename: Hariprasad
authors.initials: H
authors.lastname: Gali
mesh_terms: D020533:Angiogenesis Inhibitors / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D001943:Breast Neoplasms / Q000098:blood supply / Q000188:drug therapy; D018826:CD13 Antigens / Q000037:antagonists & inhibitors; D045744:Cell Line, Tumor; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000098:blood supply; D005260:Female; D006801:Humans; D008807:Mice, Inbred BALB C; D018919:Neovascularization, Physiologic / Q000187:drug effects; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D060327:Solid-Phase Synthesis Techniques / Q000379:methods; D023041:Xenograft Model Antitumor Assays
chemical_list: D020533:Angiogenesis Inhibitors; D009842:Oligopeptides; C063432:probestin; D018826:CD13 Antigens