title: A protein kinase assay based on FRET between quantum dots and fluorescently-labeled peptides.
abstract: A novel protein kinase assay was developed, based on FRET between QDs and fluorescently-labeled substrate peptides. The negatively charged QDs recognize the change in net charge of the peptide upon phosphorylation. Despite its simple mechanism, this assay is sensitive and robust enough to be applied to the evaluation of protein kinase inhibitors.
authors.affiliation: Graduate School of Systems Life Sciences, Kyushu University, 744 Motooka, Nishi-Ku, Fukuoka 819-0395, Japan.
authors.forename: Shujiro
authors.initials: S
authors.lastname: Shiosaki
authors.forename: Takanobu
authors.initials: T
authors.lastname: Nobori
authors.forename: Takeshi
authors.initials: T
authors.lastname: Mori
authors.forename: Riki
authors.initials: R
authors.lastname: Toita
authors.forename: Yuta
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Chan Woo
authors.initials: CW
authors.lastname: Kim
authors.forename: Tatsuhiro
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Takuro
authors.initials: T
authors.lastname: Niidome
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Katayama
mesh_terms: D004305:Dose-Response Relationship, Drug; D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer; D010455:Peptides / Q000737:chemistry*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D011494:Protein Kinases / Q000032:analysis* / Q000378:metabolism; D045663:Quantum Dots; D013329:Structure-Activity Relationship
chemical_list: D010455:Peptides; D047428:Protein Kinase Inhibitors; D011494:Protein Kinases