title: Hard X-ray nanoprobe investigations of the subtissue metal distributions within Daphnia magna.
abstract: The unique potential of nanoscale elemental imaging of major/minor and trace-level elemental distributions within thin biological tissue sections of the ecotoxicological model organism Daphnia magna is demonstrated by synchrotron radiation nano-X-ray fluorescence (nano-XRF). The applied highly specialized sample preparation method, coupled with the high spatial resolution (∼180 nm) and high X-ray photon flux (6 × 10(11) photons/s) available at the European Synchrotron Radiation Facility (ESRF) ID22NI beamline proved to be critical for the high-quality visualization of (trace-)metal distributions on the submicron level within the target structures of interest. These include the branchial sacs on the thoracic appendages (epipodites) of D. magna, which are osmoregulatory regions where ion exchange occurs. For the main element of interest (Zn), detection limits of 0.7 ppm (3 ag) was reached in fast-scanning mode using an acquisition time of 0.3 s/pixel. As demonstrated, synchrotron radiation nano-XRF revealed the elemental distributions of Ca, Fe, and Zn within this osmoregulatory region on the submicron scale, aiding the exploration of possible detoxification mechanisms of Zn within D. magna at the subtissue level.
authors.affiliation: Department of Analytical Chemistry, Ghent University, Krijgslaan 281 (S12), 9000, Ghent, Belgium. Bjorn.DeSamber@UGent.be
authors.forename: B
authors.initials: B
authors.lastname: De Samber
authors.forename: K A C
authors.initials: KA
authors.lastname: De Schamphelaere
authors.forename: C R
authors.initials: CR
authors.lastname: Janssen
authors.forename: B
authors.initials: B
authors.lastname: Vekemans
authors.forename: R
authors.initials: R
authors.lastname: De Rycke
authors.forename: G
authors.initials: G
authors.lastname: Martinez-Criado
authors.forename: R
authors.initials: R
authors.lastname: Tucoulou
authors.forename: P
authors.initials: P
authors.lastname: Cloetens
authors.forename: L
authors.initials: L
authors.lastname: Vincze
mesh_terms: D000818:Animals; D002118:Calcium / Q000032:analysis / Q000493:pharmacokinetics; D002138:Calibration; D003621:Daphnia / Q000033:anatomy & histology / Q000737:chemistry* / Q000187:drug effects; D054750:Ecotoxicology / Q000379:methods*; D004867:Equipment Design; D005453:Fluorescence; D007501:Iron / Q000032:analysis / Q000493:pharmacokinetics; D057230:Limit of Detection; D008670:Metals / Q000032:analysis / Q000493:pharmacokinetics*; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods*; D017356:Synchrotrons; D014018:Tissue Distribution; D014965:X-Rays; D015032:Zinc / Q000032:analysis / Q000493:pharmacokinetics
chemical_list: D008670:Metals; D007501:Iron; D015032:Zinc; D002118:Calcium