title: Oseltamivir-resistant influenza viruses isolated in South Korea from 2005 to 2010.
abstract: South Korean isolates of oseltamivir-resistant influenza viruses from 2005-2010 were investigated with a total 491 influenza viruses identified from 1702 specimens. Neuraminidase genes from 342 influenza viruses (71 A/H1N1, 74 pandemic A/H1N1 2009, 117 A/H3N2, and 80 B) were analyzed by RT-PCR with molecular markers for oseltamivir resistance. The H274Y mutation in the NA protein was identified in 100 % (n=40) of A/H1N1 viruses circulating in 2008-2009. Influenza A/H1N1 viruses harboring the H274Y substitution exhibited, on average, a 626-fold reduction in oseltamivir susceptibility and clustered with the A/Norway/1736/2007 strain. Close and timely monitoring for resistance to clinically available influenza antivirals should be consistently performed.
authors.affiliation: Gyeonggi-do Institute of Health and Environment, Suwon, Gyeonggi-do, South Korea.
authors.forename: Han-Gil
authors.initials: HG
authors.lastname: Cho
authors.forename: Jang-Hoon
authors.initials: JH
authors.lastname: Choi
authors.forename: Hyun-Kyung
authors.initials: HK
authors.lastname: Lee
authors.forename: Su-Kyoung
authors.initials: SK
authors.lastname: Mun
authors.forename: Jong-Bok
authors.initials: JB
authors.lastname: Lee
authors.forename: Eek-Hoon
authors.initials: EH
authors.lastname: Jho
authors.forename: Chun
authors.initials: C
authors.lastname: Kang
authors.forename: Young-Hee
authors.initials: YH
authors.lastname: Lim
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D024882:Drug Resistance, Viral; D006801:Humans; D053118:Influenza A Virus, H1N1 Subtype / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D053122:Influenza A Virus, H3N2 Subtype / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D009981:Influenza B virus / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D007251:Influenza, Human / Q000453:epidemiology* / Q000821:virology*; D009154:Mutation; D009439:Neuraminidase / Q000235:genetics*; D009975:Orthomyxoviridae / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification*; D053139:Oseltamivir / Q000494:pharmacology*; D010802:Phylogeny; D056910:Republic of Korea / Q000453:epidemiology; D014764:Viral Proteins / Q000235:genetics
chemical_list: D000998:Antiviral Agents; D014764:Viral Proteins; D053139:Oseltamivir; D009439:Neuraminidase