title: Thyroid endocrine disruption in zebrafish larvae following exposure to hexaconazole and tebuconazole.
abstract: The widely used triazole fungicides have the potential to disrupt endocrine system, but little is known of such effects or underlying mechanisms of hexaconazole (HEX) and tebuconazole (TEB) in fish. In the present study, zebrafish (Danio rerio) embryos were exposed to various concentrations of HEX (0.625, 1.25 and 2.5 mg/L) and TEB (1, 2 and 4 mg/L) from fertilization to 120 h post-fertilization (hpf). The whole body content of thyroid hormone and transcription of genes in the hypothalamic-pituitary-thyroid (HPT) axis were analyzed. The results showed that thyroxine (T4) levels were significantly decreased, while triiodothyronine (T3) concentrations were significantly increased after exposure to HEX and TEB, indicating thyroid endocrine disruption. Exposure to HEX significantly induced the transcription of all the measured genes (i.e., corticotrophin-releasing hormone (CRH), thyroid-stimulating hormone (TSHβ), sodium/iodide symporter (NIS), transthyretin (TTR), uridine diphosphate glucuronosyltransferase (UGT1ab), thyronine deiodinase (Dio1 and Dio2), thyroid hormone receptors (TRα and TRβ) in the HPT axis, but did not affect the transcription of thyroglobulin (TG). However, TEB exposure resulted in the upregulation of all the measured genes, excepting that TG, Dio1and TRα had not changed significantly. The overall results indicated that exposure to HEX and TEB could alter thyroid hormone levels as well as gene transcription in the HPT axis in zebrafish larvae.
authors.affiliation: Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310029, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Yu
authors.forename: Mengli
authors.initials: M
authors.lastname: Chen
authors.forename: Yihua
authors.initials: Y
authors.lastname: Liu
authors.forename: Wenjun
authors.initials: W
authors.lastname: Gui
authors.forename: Guonian
authors.initials: G
authors.lastname: Zhu
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D052244:Endocrine Disruptors / Q000633:toxicity*; D004797:Enzyme-Linked Immunosorbent Assay; D005786:Gene Expression Regulation / Q000187:drug effects*; D007030:Hypothalamo-Hypophyseal System / Q000378:metabolism*; D007814:Larva / Q000187:drug effects / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D013961:Thyroid Gland / Q000187:drug effects* / Q000378:metabolism; D013974:Thyroxine / Q000378:metabolism; D014230:Triazoles / Q000633:toxicity*; D014284:Triiodothyronine / Q000378:metabolism; D015027:Zebrafish / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D052244:Endocrine Disruptors; D014230:Triazoles; D014284:Triiodothyronine; C087114:tebuconazole; D003346:Corticotropin-Releasing Hormone; D013974:Thyroxine; C409722:hexaconazole