title: Gene discovery in the tropical scallop Nodipecten nodosus: construction and sequencing of a normalized cDNA library.
abstract: We report the construction and characterization of a normalized cDNA library from the digestive gland of the marine bivalve Nodipecten nodosus, a commercially valuable tropical scallop. A total of 288 clones were sequenced, and 250 unique sequences were obtained. The cDNA library showed a small sequence redundancy (2.3%) and high numbers of recombinant (99.9%) and independent clones (2.0 × 10(6) cfu), indicating that the cDNA library generated in this study is a profitable resource for efficient gene discovery for N. nodosus. EST functional annotation by Gene Ontology term assignment revealed the identification of sequences potentially involved in aquaculture and ecotoxicology relevant processes such as apoptosis, growth, lipid metabolism, reproduction, development, response to stress and immunity.
authors.affiliation: Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho 373, CSS, Bl. G, 21949902 Rio de Janeiro, Brazil. Electronic address: juliana@biof.ufrj.br.
authors.forename: Juliana Alves
authors.initials: JA
authors.lastname: Americo
authors.forename: Francesco
authors.initials: F
authors.lastname: Dondero
authors.forename: Milton Ozório
authors.initials: MO
authors.lastname: Moraes
authors.forename: Silvana
authors.initials: S
authors.lastname: Allodi
authors.forename: Mauro
authors.initials: M
authors.lastname: de Freitas Rebelo
mesh_terms: D000818:Animals; D020224:Expressed Sequence Tags; D015723:Gene Library; D005796:Genes / Q000235:genetics*; D056726:Genetic Association Studies; D008969:Molecular Sequence Data; D049897:Pectinidae / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014329:Tropical Climate
keywords: Aquaculture; EST; Lion's Paw scallop; Stress; mRNA