title: Mass measurement of polyphosphoinositides by thin-layer and gas chromatography.
abstract: Phosphoinositides derive from the phospholipid, phosphatidylinositol (PtdIns), by phosphorylation of the inositol ring in the lipid head group. The determination of phosphoinositide species is a particular challenge, because the structurally similar inositolphosphate-head groups must be analyzed as well as the lipid-associated fatty acids. The method presented in this chapter consists of two steps: First phosphoinositides are separated by thin-layer chromatography (TLC) according to their characteristic head groups and the individual lipids are isolated. Second, the fatty acids associated with each isolated lipid are analyzed using a gas-chromatograph (GC). The combination of these two classical methods for lipid analysis, TLC and GC, provides a cost-efficient and reliable alternative to lipidomics approaches requiring more extensive instrumentation.
authors.affiliation: Department of Cellular Biochemistry, Institute for Biochemistry and Biotechnology, Martin-Luther-University Halle-Wittenberg, Halle, Germany.
authors.forename: Mareike
authors.initials: M
authors.lastname: Heilmann
authors.forename: Ingo
authors.initials: I
authors.lastname: Heilmann
mesh_terms: D000143:Acids / Q000737:chemistry; D002849:Chromatography, Gas / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods*; D004951:Esterification; D005227:Fatty Acids / Q000378:metabolism; D008970:Molecular Weight; D018129:Phosphatidylinositol Phosphates / Q000737:chemistry* / Q000302:isolation & purification; D010944:Plants / Q000378:metabolism; D012015:Reference Standards; D012822:Silicon Dioxide; D012996:Solutions; D013223:Statistics as Topic
chemical_list: D000143:Acids; D005227:Fatty Acids; D018129:Phosphatidylinositol Phosphates; D012996:Solutions; D012822:Silicon Dioxide