title: [Changes in gene expression profiles in multiple myeloma cells after knocking-down SNF5, a core subunit of SWI/SNF complex].
abstract: OBJECTIVE
  To investigate the changes in the gene expression profiles in a multiple myeloma cell line after knocking-down the expression of SNF5, a core component of the SWI/SNF chromatin remodeling complex.
  
  
  METHODS
  The total RNA were extracted from tetracycline-inducible SNF5 knockdown (KD) cell line derived from KM3 cells for gene expression profiling by affymetrix microarray and bioinformatic analysis.
  
  
  RESULTS
  Knockdown of SNF5 inhibited KM3 cell proliferation. A total of 545 genes were found to be differentially expressed in the cells with SNF5 knockdown, among which 214 were up-regulated and 331 were down-regulated.
  
  
  CONCLUSIONS
  SNF5 is essential for the growth of multiple myeloma cells and can regulate the expression of the genes associated with cell growth and apoptosis.
authors.affiliation: Department of Hematology, Zhejiang University, Hangzhou, China. xstyingying@163.com
authors.forename: Yingying
authors.initials: Y
authors.lastname: Xie
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D009101:Multiple Myeloma / Q000235:genetics* / Q000473:pathology; D034741:RNA, Small Interfering; D000071796:SMARCB1 Protein; D014157:Transcription Factors / Q000235:genetics*; D059467:Transcriptome
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; D034741:RNA, Small Interfering; D000071796:SMARCB1 Protein; C513266:SMARCB1 protein, human; D014157:Transcription Factors