title: Inhibitory activity of chemical constituents from Arenaria serpyllifolia on nitric oxide production.
abstract: Five new compounds, including one new xanthone, 1-hydroxy-5-methoxyxanthone 6-O-β-D-glucopyranoside (1), one new lignan, 3-(β-D-glucopyranosyloxymethyl)-2-(4-hydroxy-3-methoxyphenyl)-5-(3-acetoxypropyl)-7-methoxy-(2R,3S)-dihydrobenzofuran (2), and three new γ-pyrones, japonicumone A 4'-O-β-D-glucopyranoside (3), japonicumone B 3'-O-β-D-glucopyranoside (4), and japonicumone B 4'-O-β-D-glucopyranoside (5), together with eight known compounds (6-13) were isolated from the whole plants of Arenaria serpyllifolia. Their structures were elucidated on the basis of extensive spectroscopic analysis (UV, IR, HRESIMS, 1D- and 2D-NMR, and CD) as well as chemical methods. The isolated compounds were evaluated for their inhibitory effects on nitric oxide production in lipopolysaccharide-activated RAW 264.7 macrophages. Compounds 1-5, sacranoside A (9), and pedunculoside (13) showed potential nitric oxide inhibitory activities with IC50 values ranging from 14.92 µM to 52.23 µM.
authors.affiliation: State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing, PR China.
authors.forename: Guan-Shen
authors.initials: GS
authors.lastname: Zhou
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Ma
authors.forename: Ke-Wu
authors.initials: KW
authors.lastname: Zeng
authors.forename: Peng-Fei
authors.initials: PF
authors.lastname: Tu
authors.forename: Yong
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D000818:Animals; D031243:Arenaria Plant / Q000737:chemistry*; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D009569:Nitric Oxide / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D013057:Spectrum Analysis
chemical_list: D010936:Plant Extracts; D009569:Nitric Oxide