title: N-Acetylglucosamine modulates function of the skin fibroblasts.
abstract: BACKGROUND
  Fibroblasts are an important component of the skin determining its properties. N-Acetylglucosamine (NAG) is the substrate for hyaluronan synthesis, and it also has anti-inflammatory and anti-senescent activity in mesothelial cells.
  
  
  METHODS
  We tested in in vitro-cultured human skin fibroblasts how supplementation of culture medium with NAG 10 mmol L(-1) changes properties of these cells.
  
  
  RESULTS
  Fibroblasts cultured in presence of NAG produced more proteins and that was mainly due to increased synthesis of collagen (+33% vs. control, P < 0.05). Hyaluronan synthesis was increased (+107% vs. control, P < 0.001), but interleukin-6 synthesis was reduced (-22% vs. control, P < 0.05). Fibroblasts cultured in medium with NAG 10 mmol L(-1) demonstrated improved ability to heal the injured layer of cells (+34% vs. control, P < 0.05). Additionally senescence of fibroblasts undergoing replicative ageing in the presence of NAG was less pronounced, as reflected by smaller increase in the population doubling time (-70% vs. control, P < 0.05).
  
  
  CONCLUSIONS
  We conclude that NAG induced changes in the skin fibroblasts' properties maybe important for prevention of the age-dependent changes in its structure and function.
authors.affiliation: Department of Pathophysiology, Poznan University of Medical Sciences, Poznan, Poland.
authors.forename: A
authors.initials: A
authors.lastname: Połubinska
authors.forename: J
authors.initials: J
authors.lastname: Cwalinski
authors.forename: E
authors.initials: E
authors.lastname: Baum
authors.forename: A
authors.initials: A
authors.lastname: Bręborowicz
mesh_terms: D000117:Acetylglucosamine / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D003094:Collagen / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism*; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D012867:Skin / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D015850:Interleukin-6; D006820:Hyaluronic Acid; D003094:Collagen; D000117:Acetylglucosamine
keywords: N-Acetylglucosamine; collagen; dermal fibroblasts; hyaluronan; inflammation; senescence