title: Single-walled carbon nanotubes as specific targeting and Raman spectroscopic agents for detection and discrimination of single human breast cancer cells.
abstract: Raman active nano-complex agents based on single-walled carbon nanotubes (SWCNTs) are prepared and used for the swift and specific detection of breast cancer cells. SWCNTs are functionalized to bond covalently with the anti-epithelial cell adhesion molecule (anti-EpCAM) antibody, which is specific to the highly expressed EpCAM antigen on the surface of breast cancer cells (MCF-7), but not on normal cells. The Raman nano-complexes demonstrate excellent ability to detect in vitro single breast cancer cells (MCF-7) and discriminate between them and normal fibroblast cells during the first 30 min of the targeting process. Raman linearity scanning is collected from a monolayer cell mixture, including both cancer cells and normal cells incubated with anti-EpCAM-SWCNTs, using a 633-nm laser excitation. The results shows that the Raman signal collected from targeted MCF-7 cells is extremely high, whereas there is little signal from the normal cells.
authors.affiliation: University of Arkansas at Little Rock, Center for Integrative Nanotechnology Sciences, Little Rock, AR 72204, USA.
authors.forename: Zeid A
authors.initials: ZA
authors.lastname: Nima
authors.forename: Meena W
authors.initials: MW
authors.lastname: Mahmood
authors.forename: Alokita
authors.initials: A
authors.lastname: Karmakar
authors.forename: Thikra
authors.initials: T
authors.lastname: Mustafa
authors.forename: Shawn
authors.initials: S
authors.lastname: Bourdo
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
authors.forename: Alexandru S
authors.initials: AS
authors.lastname: Biris
mesh_terms: D000951:Antigens, Neoplasm / Q000737:chemistry / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000737:chemistry / Q000378:metabolism; D001943:Breast Neoplasms / Q000737:chemistry* / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000378:metabolism; D002460:Cell Line; D000071858:Epithelial Cell Adhesion Molecule; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D061986:MCF-7 Cells; D037742:Nanotubes, Carbon / Q000737:chemistry*; D059010:Single-Cell Analysis / Q000379:methods*; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules; D000071858:Epithelial Cell Adhesion Molecule; D037742:Nanotubes, Carbon