title: Nanosecond pulsed electric field stimulation of reactive oxygen species in human pancreatic cancer cells is Ca(2+)-dependent.
abstract: The cellular response to 100 ns pulsed electric fields (nsPEF) exposure includes the formation of transient nanopores in the plasma membrane and organelle membranes, an immediate increase in intracellular Ca(2+), an increase in reactive oxygen species (ROS), DNA fragmentation and caspase activation. 100 ns, 30 kV/cm nsPEF stimulates an increase in ROS proportional to the pulse number. This increase is inhibited by the anti-oxidant, Trolox, as well as the presence of Ca(2+) chelators in the intracellular and extracellular media. This suggests that the nsPEF-triggered Ca(2+) increase is required for ROS generation.
authors.affiliation: BioElectroMed Corp., 849 Mitten Rd., Suite 104, Burlingame, CA 94010, USA. rich@bioelectromed.com
authors.forename: Richard
authors.initials: R
authors.lastname: Nuccitelli
authors.forename: Kaying
authors.initials: K
authors.lastname: Lui
authors.forename: Mark
authors.initials: M
authors.lastname: Kreis
authors.forename: Brian
authors.initials: B
authors.lastname: Athos
authors.forename: Pamela
authors.initials: P
authors.lastname: Nuccitelli
mesh_terms: D002118:Calcium / Q000378:metabolism*; D045744:Cell Line, Tumor; D004558:Electric Stimulation / Q000379:methods*; D006801:Humans; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species; D002118:Calcium