title: T helper (Th) 1 and Th2 cytokine expression profile in dengue and malaria infection using magnetic bead-based bio-plex assay.
abstract: Dengue and malaria infections are two very common vector-borne diseases annually affecting millions of people around the world. Both diseases show a variety of clinical presentations, ranging from mild symptoms of dengue fever (DF) to severe dengue hemorrhagic fever (DHF) in dengue infection, and low and high parasitemia in malaria infection. T helper (Th)1 and Th2 cytokine expressions in mild and severe forms of dengue virus type-2 (DENV-2) and Plasmodium falciparum infection, were compared to normal human sera using high throughput magnetic bead-based Bio-Plex assay. A significant elevation of Th1 and Th2 cytokines expression [interleukin (IL)-2, IL-4, IL-5, IL-10, IL-13, granulocyte-macrophage colony-stimulating factor (GM-CSF), interferon (IFN)-gamma, and tumor necrosis factor (TNF)-alpha] was detected in DENV-2 and P. falciparum malaria infections compared with normal controls (p < 0.05). DENV-2 infection showed a slight higher expression of Th1 and Th2 cytokines in DHF than DF, except for IL-13. In P. falciparum infection, high parasitemia showed a significantly higher expression of IL-4, IL-10, GM-CSF, and TNF-alpha (p < 0.05). Both DENV-2 and P. falciparum malaria infections manifested high IL-10 expression, greatest among the cytokines examined, and in the severe forms of infection. The results of this study should lead to a better understanding of pathogenesis of dengue infection and P. falciparum malaria.
authors.affiliation: Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Pannamas
authors.initials: P
authors.lastname: Maneekan
authors.forename: Pornsawan
authors.initials: P
authors.lastname: Leaungwutiwong
authors.forename: Dorothee
authors.initials: D
authors.lastname: Misse
authors.forename: Natthanej
authors.initials: N
authors.lastname: Luplertlop
mesh_terms: D016207:Cytokines / Q000096:biosynthesis*; D003715:Dengue / Q000453:epidemiology / Q000276:immunology* / Q000378:metabolism; D003716:Dengue Virus / Q000276:immunology; D006801:Humans; D018189:Immunomagnetic Separation; D016778:Malaria, Falciparum / Q000453:epidemiology / Q000276:immunology* / Q000378:metabolism; D010963:Plasmodium falciparum / Q000276:immunology; D012720:Severity of Illness Index; D018417:Th1 Cells / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*; D013785:Thailand / Q000453:epidemiology
chemical_list: D016207:Cytokines