title: Structural and functional characterization of receptor kinases with nucleotide cyclase activity.
abstract: There has been an increase in the identification and characterization of plant receptor kinases possessing nucleotide cyclase activity. This has necessitated the development of robust methodologies for the structural and functional characterization of this biologically important family of proteins. Here we outline some of the techniques that can be effectively used in the characterization of this bifunctional family of proteins.
authors.affiliation: Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, VIC, Australia.
authors.forename: Victor
authors.initials: V
authors.lastname: Muleya
authors.forename: Janet I
authors.initials: JI
authors.lastname: Wheeler
authors.forename: Helen R
authors.initials: HR
authors.lastname: Irving
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000201:enzymology*; D003001:Cloning, Molecular; D003432:Cross-Linking Reagents / Q000494:pharmacology; D006152:Cyclic GMP / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005453:Fluorescence; D006162:Guanylate Cyclase / Q000378:metabolism*; D006801:Humans; D010766:Phosphorylation / Q000187:drug effects; D020794:Receptor Protein-Tyrosine Kinases / Q000737:chemistry* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D003432:Cross-Linking Reagents; D011994:Recombinant Proteins; D020794:Receptor Protein-Tyrosine Kinases; D006162:Guanylate Cyclase; D006152:Cyclic GMP