title: Microencapsulated probiotics using emulsification technique coupled with internal or external gelation process.
abstract: Alginate-chitosan microcapsules containing probiotics (Yeast, Y235) were prepared by emulsification/external gelation and emulsification/internal gelation techniques respectively. The gel beads by external gelation showed asymmetrical structure, but those by internal gelation showed symmetrical structure in morphology. The cell viability was approximately 80% for these two techniques. However, during cell culture process, emulsification/internal gelation microcapsules showed higher cell growth and lower cell leakage. Moreover, the survival rate of entrapped low density cells with culture (ELDCwc) increased obviously than that directly entrapped high density cells (dEHDC) and free cells when keeping in simulated gastrointestinal conditions. It indicated the growth process of cells in microcapsule was important and beneficial to keep enough active probiotics under harmful environment stress. Therefore, the emulsification/internal gelation technique was the preferred method for application in food or biotechnological industries.
authors.affiliation: Laboratory of Biomedical Materials Engineering, Dalian Institute of Chemical Physics (DICP), Chinese Academy of Sciences (CAS), Dalian 116023, PR China.
authors.forename: Huiyi
authors.initials: H
authors.lastname: Song
authors.forename: Weiting
authors.initials: W
authors.lastname: Yu
authors.forename: Meng
authors.initials: M
authors.lastname: Gao
authors.forename: Xiudong
authors.initials: X
authors.lastname: Liu
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Ma
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D004339:Drug Compounding; D004655:Emulsions; D005782:Gels; D019936:Probiotics / Q000737:chemistry*; D015003:Yeasts / Q000737:chemistry*
chemical_list: D004655:Emulsions; D005782:Gels