title: [Influence of serum containing Liuwei Dihuang decoction (see text) on proliferation of osteoblasts under stretch-stress environment].
abstract: OBJECTIVE
  To study influence of serum containing Liuwei Dihuang decoction (see text) on proliferation and differentiation of osteoblast form neonatal SD rats cultured in vitro at different times and different stretch stress.
  
  
  METHODS
  After osteoblast cultured for 24 hours in the serum containing Liuwei Dihuang decoction (see text) and serum in control group, the 0.5 Hz frequency, 6% and 12% stretch-stress were added. The MTT1 and the activity of ALP were measured at the 12th and 24th hours, and the data were analyzed.
  
  
  RESULTS
  1. In the environment of stretch stress to the frequency of 0.5 Hz, and stretched for 24 hours, the osteoblast was stimulated under elongation rate of 6% and 12%; the proliferation and differentiation of osteoblast was more active under elongation rate of 12% than that of 6%. 2. There were no stimulating effects on osteoblast proliferation and differentiation of serum containing Liuwei Dihuiang decoction (see text) acted on osteoblast cells of SD rats cultured in vitro for a shot time.
  
  
  CONCLUSIONS
  Stretch stress environment can enhance osteoblast proliferation and differentiation cultured in vitro.
authors.affiliation: Zhejing University of Traditional Chinese Medicine, Hangzhou, Zhejiang, China.
authors.forename: Wan
authors.initials: W
authors.lastname: Cheng
authors.forename: Xiao-Kang
authors.initials: XK
authors.lastname: Tang
authors.forename: Hang
authors.initials: H
authors.lastname: Ying
authors.forename: Min
authors.initials: M
authors.lastname: Li
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008297:Male; D010006:Osteoblasts / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D044967:Serum; D013314:Stress, Mechanical
chemical_list: D004365:Drugs, Chinese Herbal; C042579:Liuwei Dihuang Decoction; D000469:Alkaline Phosphatase