title: Genetic differ in TLR4 gene polymorphisms and expression involved in Salmonella natural and artificial infection respectively in Chinese native chicken breeds.
abstract: Salmonella enteritidis (SE) is a foodborne pathogen that negatively affects both animal and human health. Genetic variations in response to pathogenic SE colonization or to SE vaccination were measured in chicken resource populations. Toll-like receptor 4 (TLR4) is part of a group of evolutionarily conserved pattern recognition receptors involved in the activation of the immune system in response to various pathogens and in the innate defense against infection. In this study, TLR4 was investigated the association of TLR4 gene polymorphisms with Salmonella natural infection situation of birds from two distinct Chinese genetic breeds. One SNP G1894C in the second intron of chicken TLR4 (chTLR4) was scanned in the two hens breed, which showed significant association with Salmonella natural infection situation (P<0.05). Genetic variations in response to pathogenic SE colonization also existed in distinct Chinese chicken resource population. In this study, mRNA expression of TLR4 was detected to investigate the association with the effect of artificial SE challenge in heterophil granulocytes and spleen of chicks from two distinct Chinese genetic breeds at 1, 3 and 10 day post-infection during the acute infection period. It clearly showed that young chicks' response to SE infection was regulated by TLR4 mRNA expression. The results suggest that genetics, time, gender, and interactions among these factors, play important roles in TLR4 mRNA basic values and copies modulation of SE mediated immune response in distinct Chinese chickens.
authors.affiliation: Jiangsu Provincial Key Laboratory of Poultry Genetics & Breeding, Institute of Poultry Science of Jiangsu Province, Yangzhou, 225125, Jiangsu, People's Republic of China. lhfxf_002@yahoo.com.cn
authors.forename: H F
authors.initials: HF
authors.lastname: Li
authors.forename: Y
authors.initials: Y
authors.lastname: Hu
authors.forename: H
authors.initials: H
authors.lastname: Hu
authors.forename: C
authors.initials: C
authors.lastname: Song
authors.forename: J T
authors.initials: JT
authors.lastname: Shu
authors.forename: C H
authors.initials: CH
authors.lastname: Zhu
authors.forename: S J
authors.initials: SJ
authors.lastname: Zhang
authors.forename: J H
authors.initials: JH
authors.lastname: Fan
authors.forename: W W
authors.initials: WW
authors.lastname: Chen
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002645:Chickens; D002681:China; D017931:DNA Primers / Q000235:genetics; D014644:Genetic Variation; D006098:Granulocytes / Q000276:immunology; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D018807:Polymorphism, Single-Stranded Conformational; D011201:Poultry Diseases / Q000235:genetics / Q000276:immunology* / Q000382:microbiology*; D060888:Real-Time Polymerase Chain Reaction; D012481:Salmonella Infections, Animal / Q000235:genetics / Q000276:immunology*; D012477:Salmonella enteritidis / Q000276:immunology*; D012737:Sex Factors; D013045:Species Specificity; D013154:Spleen / Q000276:immunology; D013997:Time Factors; D051197:Toll-Like Receptor 4 / Q000235:genetics* / Q000378:metabolism
chemical_list: D017931:DNA Primers; D051197:Toll-Like Receptor 4