title: Differential detection of Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii in fecal samples by nested PCR in the United Arab Emirates (UAE).
abstract: Amoebiasis is one of the most important infectious diseases afflicting mainly tropical and subtropical countries. This study was carried out in the Sharjah Emirate, UAE in order to accurately detect and differentiate Entamoeba histolytica, Entamoeba dispar and E. moshkovskii in fecal samples collected from the Sharjah municipality public health clinic by ELISA and nested polymerase chain reaction (PCR). One hundred and twenty specimens were examined and the PCR was positive for E. histolytica, E. dispar and E. moshkovskii (collectively referred to as Entamoeba complex) in 19.2% (23 out of 120). Of those, 10% (12/120) were mono - infection with E. histolytica; 2.5% (3/120) with E. dispar; and 2.5% (3/120) E. moshkovskii. The nested PCR also detected mixed infections by both E. histolytica and E. dispar in 3.3% (4/120) and E. dispar and E. moshkovskii in 0.8% (1/120). The TechLab ELISA kit failed to detect E. histolytica in any of the E. histolytica PCR positive samples. Overall, the percentage of E. histolytica including those found in mixed infections was 13.3% (16/120). Compared to nested PCR, microscopy was found to have an overall sensitivity of 52.2% and a specificity of 75.2% for detection of Entamoeba complex. The present study indicates that E. histolytica is present in the UAE with an average incidence rate of 13.3%. However, larger studies need to be conducted in order to confirm these findings. We propose the use of PCR in both the routine diagnosis of amoebiasis and epidemiological survey in the UAE.
authors.affiliation: Department of Medical Laboratory Technology, College of Health Sciences, University of Sharjah, Sharjah, UAE.
authors.forename: Ali
authors.initials: A
authors.lastname: ElBakri
authors.forename: Amidou
authors.initials: A
authors.lastname: Samie
authors.forename: Sinda
authors.initials: S
authors.lastname: Ezzedine
authors.forename: Raed Abu
authors.initials: RA
authors.lastname: Odeh
mesh_terms: D000953:Antigens, Protozoan / Q000032:analysis; D016054:DNA, Protozoan / Q000032:analysis; D004404:Dysentery, Amebic / Q000175:diagnosis* / Q000453:epidemiology / Q000469:parasitology; D004747:Entamoeba / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D004748:Entamoeba histolytica / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D004749:Entamoebiasis / Q000175:diagnosis* / Q000453:epidemiology / Q000469:parasitology; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology*; D006801:Humans; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D014479:United Arab Emirates / Q000453:epidemiology
chemical_list: D000953:Antigens, Protozoan; D016054:DNA, Protozoan