title: [Archaeal community structure and diversity in Urumqi No. 10 cold sulfur spring analyzed by culture-independent approach].
abstract: OBJECTIVE
  We explored the composition and diversity of archaea in a cold sulfur spring water in Xinjiang earthquake fault zone.
  
  
  METHODS
  Environmental total DNA was extracted directly with enzymatic lysis method from a cold sulfur spring water. We constructed clone library of 16S rRNA gene amplified with archaeal-specific primers. A total of 115 positive clones were selected randomly from the library and identified by restriction length polymorphism (RFLP) with enzyme Alu I and Afa I. The unique RFLP patterns corresponded clones were selected for sequencing, BLAS alignment and constructing 16S rRNA gene phylogenetic tree.
  
  
  RESULTS
  In total, 44 operational taxonomic units (OTUs) were determined from the library. BLAST and phylogenetic analysis indicated that these OTUs were affiliated with Euryarchaeota (94.78%) and Thaumarchaeota (4.35%). Only one Thaumarchaeotal clone was detected and most related to the genus Nitrosopumilus with 93% similarity. Euryarchaeotal clones were abundant and diverse. Of them, 42.61% of clones belonged to RC-V cluster; 13.91% of clones, 20.87% of clones were classified into LDS cluster and Methanomicrobiales respectively; 4.35% of clones had high similarity with ANME-1a-FW, which were involved in Anaerobic oxidation of methane (AOM). In addition, we also detected some (13.05%) unknown Euryarchaotal clones.
  
  
  CONCLUSIONS
  Euryarchaeota in the environment were diverse, and possibly with a large fraction of potential novel species.
authors.affiliation: College of Life Science and Technology of Xinjiang University, Urumqi 830046, China. 15276702381@163.com
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Zeng
authors.forename: Yunus
authors.initials: Y
authors.lastname: Zulipiya
authors.forename: Xiaoqi
authors.initials: X
authors.lastname: Gao
authors.forename: Xiuhuang
authors.initials: X
authors.lastname: Dong
authors.forename: Juan
authors.initials: J
authors.lastname: Xue
authors.forename: Kai
authors.initials: K
authors.lastname: Lou
mesh_terms: D001105:Archaea / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D044822:Biodiversity; D002681:China; D017931:DNA Primers / Q000235:genetics; D019641:DNA, Archaeal / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D019605:Euryarchaeota / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D015723:Gene Library; D008969:Molecular Sequence Data; D060985:Natural Springs / Q000382:microbiology; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D019642:RNA, Archaeal / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013455:Sulfur
chemical_list: D017931:DNA Primers; D019641:DNA, Archaeal; D004275:DNA, Ribosomal; D019642:RNA, Archaeal; D012336:RNA, Ribosomal, 16S; D013455:Sulfur