title: Halomonas sp. BS4, A biosurfactant producing halophilic bacterium isolated from solar salt works in India and their biomedical importance.
abstract: Halophilic bacteria were isolated from Thamaraikulam solar salt works in India. After routine biosurfactant screening by various methods, the biosurfactant producing bacteria, Halomonas sp BS4 was confirmed by 16 S rRNA sequencing. The growth optimization of Halomonas sp BS4 revealed their optimum growth at 8% NaCl and 6-8 pH in the growth medium. Further the partially purified biosurfactants were characterized by TLC, FTIR and GC-MS analysis. GC-MS results revealed that, the partial purified biosurfactants contain 1, 2-Ethanediamine N, N, N', N'-tetra, 8-Methyl-6-nonenamide, (Z)-9-octadecenamide and a fatty acid derivative. Pharmacological screening of antibacterial, antifungal, antiviral and anticancer assays revealed that, the biosurfactant extracted from Halomonas sp BS4 effectively controlled the human pathogenic bacteria and fungi an aquaculturally important virus, WSSV. The biosurfactant also suppressed the proliferation of mammary epithelial carcinoma cell by 46.77% at 2.5 μg concentration. Based on these findings, the present study concluded that, there is a possibility to develop eco-friendly antimicrobial and anticancer drugs from the extremophilic origin.
authors.affiliation: Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam, Kanyakumari Dist, Tamilnadu 629502, India.
authors.forename: Mariathason Birdilla Selva
authors.initials: MB
authors.lastname: Donio
authors.forename: Fernando Arul
authors.initials: FA
authors.lastname: Ronica
authors.forename: Vijayaragavan Thanga
authors.initials: VT
authors.lastname: Viji
authors.forename: Subramanian
authors.initials: S
authors.lastname: Velmurugan
authors.forename: John Selesteen Charles Adlin
authors.initials: JS
authors.lastname: Jenifer
authors.forename: Mariavincent
authors.initials: M
authors.lastname: Michaelbabu
authors.forename: Prasenjit
authors.initials: P
authors.lastname: Dhar
authors.forename: Thavasimuthu
authors.initials: T
authors.lastname: Citarasu
keywords: Antimicrobial activity; Biosurfactants; Halomonas; White spot syndrome virus (WSSV)