title: Peptide-based vector of VEGF plasmid for efficient gene delivery in vitro and vessel formation in vivo.
abstract: Critical limb ischemia is regarded as a potentially lethal disease, and the treatment effects of existing therapies are limited. Here, in order to develop a potential approach to improve the therapy effects, we designed a peptide of TAT-PKKKRKV as the vector for VEGF165 plasmid to facilitate in vivo angiogenesis. In in vitro studies, TAT-PKKKRKV with low cytotoxicity exhibited efficient transfection ability either with or without serum. Additionally, application of TAT-PKKKRKV/VEGF165 complexes in hindlimb ischemia rats obviously promoted the expression of VEGF protein, which further enhanced effective angiogenesis. The results indicated that TAT-PKKKRKV is an efficient gene vector with low toxicity both in vitro and in vivo, which has great potential for clinical gene therapy.
authors.affiliation: Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, P R China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qu
authors.forename: Si-Yong
authors.initials: SY
authors.lastname: Qin
authors.forename: Shan
authors.initials: S
authors.lastname: Ren
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Jiang
authors.forename: Ren-Xi
authors.initials: RX
authors.lastname: Zhuo
authors.forename: Xian-Zheng
authors.initials: XZ
authors.lastname: Zhang
mesh_terms: D000818:Animals; D019556:COS Cells; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D004195:Disease Models, Animal; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000737:chemistry* / Q000494:pharmacology; D057809:HEK293 Cells; D006367:HeLa Cells; D006614:Hindlimb / Q000187:drug effects* / Q000473:pathology; D006801:Humans; D007511:Ischemia / Q000188:drug therapy* / Q000473:pathology; D009842:Oligopeptides / Q000737:chemistry* / Q000494:pharmacology; D010316:Particle Size; D010957:Plasmids / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D013499:Surface Properties; D042442:Vascular Endothelial Growth Factors / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D009842:Oligopeptides; D042442:Vascular Endothelial Growth Factors