title: Expression of Opa interacting protein 5 (OIP5) is associated with tumor stage and prognosis of clear cell renal cell carcinoma.
abstract: Opa interacting protein 5 (OIP5), overexpressed in some types of human cancers, has been reported to be associated with the carcinogenesis of human cancer. However, the biological function and clinical significance of OIP5 in human Clear Cell Renal Cell Carcinoma (CCRCC) remains unknown. In the present study, we found the expression of OIP5 was markedly upregulated in surgical CCRCC specimens and CCRCC cell lines. Immunohistochemical analysis revealed that paraffin-embedded archival CCRCC specimens exhibited higher levels of OIP5 expression than normal renal tissues. Further statistical analysis suggested the upregulation of OIP5 was positively correlated with the Fuhrman grade (P = 0.02), T classification (P = 0.015), N classification (P = 0.018) and clinical stage (P = 0.035). Also, patients with high OIP5 expression dramatically exhibited shorter survival time (P = 0.001). In addition, the OIP5 expression was an independent prognostic marker of overall survival of CCRCC patients in a multivariate analysis (P = 0.008). Experimentally, we demonstrated that silencing OIP5 in CCRCC cell lines by specific siRNA clearly inhibited cell growth. In conclusion, our findings suggested that OIP5 could be a valuable marker of CCRCC progression and prognosis, and a promising therapeutic target for CCRCC.
authors.affiliation: Department of Urological Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
authors.forename: Mancheng
authors.initials: M
authors.lastname: Gong
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Xu
authors.forename: Wenjing
authors.initials: W
authors.lastname: Dong
authors.forename: Guiying
authors.initials: G
authors.lastname: Guo
authors.forename: Wenjun
authors.initials: W
authors.lastname: Ni
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Wang
authors.forename: Ruihua
authors.initials: R
authors.lastname: An
mesh_terms: D002292:Carcinoma, Renal Cell / Q000737:chemistry / Q000175:diagnosis* / Q000235:genetics*; D018797:Cell Cycle Proteins; D045744:Cell Line, Tumor; D002470:Cell Survival; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis / Q000235:genetics*; D006801:Humans; D007680:Kidney Neoplasms / Q000737:chemistry / Q000175:diagnosis* / Q000235:genetics*; D009367:Neoplasm Staging; D011379:Prognosis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; C517146:OIP5 protein, human
keywords: Clear cell renal cell carcinoma; Opa interacting protein 5 (OIP5); Prognosis; Progression; RNA interference