title: Phytochemical composition ofPotentilla anserinaL. analyzed by an integrative GC-MS and LC-MS metabolomics platform.
abstract: Potentilla anserinaL. (Rosaceae) is known for its beneficial effects of prevention of pre-menstrual syndrome (PMS). For this reasonP. anserinais processed into many food supplements and pharmaceutical preparations. Here we analyzed hydroalcoholic reference extracts and compared them with various extracts of different pharmacies using an integrative metabolomics platform comprising GC-MS and LC-MS analysis and software toolboxes for data alignment (MetMAX Beta 1.0) and multivariate statistical analysis (COVAIN 1.0). Multivariate statistics of the integrated GC-MS and LC-MS data showed strong differences between the different plant extract formulations. Different groups of compounds such as chlorogenic acid, kaempferol 3-O-rutinoside, acacetin 7-O-rutinoside, and genistein were reported for the first time in this species. The typical fragmentation pathway of the isoflavone genistein confirmed the identification of this active compound that was present with different abundances in all the extracts analyzed. As a result we have revealed that different extraction procedures from different vendors produce different chemical compositions, e.g. different genistein concentrations. Consequently, the treatment may have different effects. The integrative metabolomics platform provides the highest resolution of the phytochemical composition and a mean to define subtle differences in plant extract formulations.
authors.affiliation: Department of Pharmaceutical and Biomedical Sciences, University of Salerno, Salerno, Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Mari
authors.forename: David
authors.initials: D
authors.lastname: Lyon
authors.forename: Lena
authors.initials: L
authors.lastname: Fragner
authors.forename: Paola
authors.initials: P
authors.lastname: Montoro
authors.forename: Sonia
authors.initials: S
authors.lastname: Piacente
authors.forename: Stefanie
authors.initials: S
authors.lastname: Wienkoop
authors.forename: Volker
authors.initials: V
authors.lastname: Egelhofer
authors.forename: Wolfram
authors.initials: W
authors.lastname: Weckwerth
keywords: Flavonoids; GC-MS; Genistein; LC-MS; Mass accuracy precursor alignment (MAPA); Medicinal plants; Metabolomics