title: Quantification of diacylglycerol by mass spectrometry.
abstract: Diacylglycerol (DAG) is an important intermediate of lipid metabolism and a component of phospholipase C signal transduction. Quantification of DAG in plant membranes represents a challenging task because of its low abundance. DAG can be measured by direct infusion mass spectrometry (MS) on a quadrupole time-of-flight mass spectrometer after purification from the crude plant lipid extract via solid-phase extraction on silica columns. Different internal standards are employed to compensate for the dependence of the MS and MS/MS signals on the chain length and the presence of double bonds in the acyl moieties. Thus, using a combination of single MS and MS/MS experiments, quantitative results for the different molecular species of DAGs from Arabidopsis can be obtained.
authors.affiliation: Institute of Molecular Physiology and Biotechnology of Plants (IMBIO), University of Bonn, Bonn, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: vom Dorp
authors.forename: Isabel
authors.initials: I
authors.lastname: Dombrink
authors.forename: Peter
authors.initials: P
authors.lastname: Dörmann
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D005591:Chemical Fractionation; D004075:Diglycerides / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D013058:Mass Spectrometry / Q000379:methods*; D008970:Molecular Weight; D018515:Plant Leaves / Q000378:metabolism; D012015:Reference Standards; D052616:Solid Phase Extraction; D013223:Statistics as Topic
chemical_list: C011439:1,2-diacylglycerol; D004075:Diglycerides