title: Trans-ferulic acid-based solid lipid nanoparticles and their antioxidant effect in rat brain microsomes.
abstract: In this study, stearic acid- and stearyl ferulate-based solid lipid nanoparticles containing trans-ferulic acid (SLN-FA and SLN-SF-FA, respectively), were prepared and characterized for loading efficiency, size and shape. In addition, by using rat brain microsomes, we evaluated in vitro the antioxidant activity of these formulations against three well known initiators of lipid peroxidation, such as AAPH, NADPH/ADP-Fe(3+) and SIN-1 which in turn generate the peroxyl and perferryl radicals as well as peroxynitrite, respectively. Commercially available FA and its ethyl ester (FAEE) were used as comparators. Both SLN-FA and SLN-SF-FA dose-dependently reduced lipid peroxidation induced by the three oxidants. Interestingly, SLN-SF-FA displayed greater efficacy (EC50) and potency (maximal activity) against AAPH- and NADPH/ADP-Fe(3+)-induced lipid peroxidation. Our results support the idea that this new formulations could facilitate the uptake of FA by the cells because of their lipophilic structure, thus increasing FA bioavailability. Furthermore, stearyl ferulate-based nanoparticles could prevent the degradation of FA entrapped on their structure, making FA almost entirely available to explicate its antioxidant power once released.
authors.affiliation: Department of Pharmaceutical Sciences, University of Calabria, 87036 Arcavacata di Rende (CS), Italy.
authors.forename: Sonia
authors.initials: S
authors.lastname: Trombino
authors.forename: Roberta
authors.initials: R
authors.lastname: Cassano
authors.forename: Teresa
authors.initials: T
authors.lastname: Ferrarelli
authors.forename: Eugenio
authors.initials: E
authors.lastname: Barone
authors.forename: Nevio
authors.initials: N
authors.lastname: Picci
authors.forename: Cesare
authors.initials: C
authors.lastname: Mancuso
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry*; D001921:Brain / Q000166:cytology*; D003373:Coumaric Acids / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry*; D008297:Male; D008861:Microsomes / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D051381:Rats; D013499:Surface Properties
chemical_list: D000975:Antioxidants; D003373:Coumaric Acids; D008055:Lipids; C004999:ferulic acid