title: A dynamic database of microarray-characterized cell lines with various cytogenetic and genomic backgrounds.
abstract: The Human Genetic Cell Repository sponsored by the National Institute of General Medical Sciences (NIGMS) contains more than 11,000 cell lines and DNA samples collected from numerous individuals. All of these cell lines and DNA samples are categorized into several collections representing a variety of disease states, chromosomal abnormalities, heritable diseases, distinct human populations, and apparently healthy individuals. Many of these cell lines have previously been studied with detailed conventional cytogenetic analyses, including G-banded karyotyping and fluorescence in situ hybridization. This work was conducted by investigators at submitting institutions and scientists at Coriell Institute for Medical Research, where the NIGMS Repository is hosted. Recently, approximately 900 cell lines, mostly chosen from the Chromosomal Aberrations and Heritable Diseases collections, have been further characterized in detail at the Coriell Institute using the Affymetrix Genome-Wide Human SNP Array 6.0 to detect copy number variations and copy number neutral loss of heterozygosity. A database containing detailed cytogenetic and genomic information for these cell lines has been constructed and is freely available through several sources, such as the NIGMS Repository website and the University of California at Santa Cruz Genome Browser. As additional cell lines are analyzed and subsequently added into it, the database will be maintained dynamically.
authors.affiliation: Coriell Institute for Medical Research, Camden, New Jersey 08103, USA. ztang@coriell.org
authors.forename: Zhenya
authors.initials: Z
authors.lastname: Tang
authors.forename: Dorit S
authors.initials: DS
authors.lastname: Berlin
authors.forename: Lorraine
authors.initials: L
authors.lastname: Toji
authors.forename: Gokce A
authors.initials: GA
authors.lastname: Toruner
authors.forename: Christine
authors.initials: C
authors.lastname: Beiswanger
authors.forename: Shashikant
authors.initials: S
authors.lastname: Kulkarni
authors.forename: Christa L
authors.initials: CL
authors.lastname: Martin
authors.forename: Beverly S
authors.initials: BS
authors.lastname: Emanuel
authors.forename: Michael
authors.initials: M
authors.lastname: Christman
authors.forename: Norman P
authors.initials: NP
authors.lastname: Gerry
mesh_terms: D002460:Cell Line / Q000166:cytology* / Q000378:metabolism; D025063:Chromosome Disorders / Q000235:genetics* / Q000473:pathology; D020732:Cytogenetic Analysis; D030541:Databases, Genetic; D015894:Genome, Human; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide
keywords: FISH; ISCN 2013; chromosomal microarray analysis; karyotype