title: Identification of senkyunolide I metabolites in rats using ultra performance liquid chromatography/quadrupole-time-of-flight tandem mass spectrometry.
abstract: Ligusticum chuanxiong Hort. (Umbelliferae) has been widely prescribed to treat cardiovascular disease in China for centuries. Senkyunolide I is one of the major bioactive components in L. chuanxiong, which shows pharmacological activities against migraines and oxidative damage. In this paper, ultra performance liquid chromatography/quadrupole-time-of-flight tandem mass spectrometry (UPLC/Q-TOF-MS) was applied for the rapid analysis of senkyunolide I metabolites in rats after its intravenous administration. The non-metabolized parent compound and eighteen metabolites from drug-treated samples in rat plasma, urine and bile were identified. Our analysis indicated that methylation, hydration, epoxidation, glucuronidation and glutathione conjugation were the major pathways of senkyunolide I metabolism in vivo. This study provides important information regarding the metabolism of senkyunolide I, which will be helpful for understanding its mechanism of action. Furthermore, this work demonstrates the potential of using UPLC/Q-TOF-MS for the rapid and reliable characterization of the metabolites of natural products.
authors.affiliation: Engineering Research Center of Modern Preparation of TCM, Ministry of Education, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Yao-kun
authors.initials: YK
authors.lastname: Xiong
authors.forename: Xiao
authors.initials: X
authors.lastname: Lin
authors.forename: Shuang
authors.initials: S
authors.lastname: Liang
authors.forename: Yan-long
authors.initials: YL
authors.lastname: Hong
authors.forename: Lan
authors.initials: L
authors.lastname: Shen
authors.forename: Yi
authors.initials: Y
authors.lastname: Feng
mesh_terms: D061605:Administration, Intravenous; D000818:Animals; D001572:Benzofurans / Q000008:administration & dosage / Q000032:analysis* / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005260:Female; D030004:Ligusticum; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013997:Time Factors
chemical_list: D001572:Benzofurans; D004365:Drugs, Chinese Herbal; C576743:senkyunolide I; C101574:chuangxiong extract