title: Excretory/secretory products of Fasciola hepatica but not recombinant phosphoglycerate kinase induce death of human hepatocyte cells.
abstract: The liver fluke Fasciola hepatica infects a wide range of hosts, and has a considerable impact on the agriculture industry, mainly through infections of sheep and cattle. Further, human infection is now considered of public health importance and is hyperendemic in some regions. The fluke infection causes considerable damage to the hosts' liver. However, the mechanisms of liver destruction have not yet been completely elucidated. In the present report we incubated a human liver cell line in the presence of either F. hepatica excretory/secretory material (FhES) or recombinant phosphoglycerate kinase (FhPGK). Dosedependent cytotoxicity in the presence of FhES was observed, indicating that FhES is capable of killing human hepatocytes, supporting a role for FhES in damaging host liver cells during infection; while treatment with a recombinant intracellular protein - FhPGK, had no impact on cell survival.
authors.affiliation: Division of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Warsaw University of Life Sciences - SGGW, 8 Ciszewskiego Street, 02-786, Warsaw, Poland. piotr_baska@sggw.pl
authors.forename: Piotr
authors.initials: P
authors.lastname: Bąska
authors.forename: Luke J
authors.initials: LJ
authors.lastname: Norbury
authors.forename: Marcin
authors.initials: M
authors.lastname: Wiśniewski
authors.forename: Kamil
authors.initials: K
authors.lastname: Januszkiewicz
authors.forename: Halina
authors.initials: H
authors.lastname: Wędrychowicz
mesh_terms: D000818:Animals; D016923:Cell Death / Q000187:drug effects*; D005210:Fasciola hepatica / Q000378:metabolism / Q000472:pathogenicity*; D015801:Helminth Proteins / Q000378:metabolism / Q000494:pharmacology*; D056945:Hep G2 Cells; D022781:Hepatocytes / Q000187:drug effects* / Q000469:parasitology; D006790:Host-Parasite Interactions; D006801:Humans; D010735:Phosphoglycerate Kinase / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D015801:Helminth Proteins; D011994:Recombinant Proteins; D010735:Phosphoglycerate Kinase