title: Expression of the NOB1 gene and its clinical significance in papillary thyroid carcinoma.
abstract: OBJECTIVE
  The aim of this study was to explore the relationships between expression of the NIN1/RPN12 binding protein 1 homologue gene (NOB1) in papillary thyroid carcinoma tissue and clinicopathological variables.
  
  
  METHODS
  Expression of NOB1 in papillary thyroid carcinoma, normal thyroid and benign thyroid tumour tissue was evaluated at the mRNA and protein levels by real-time fluorescence quantitative reverse transcription-polymerase chain reaction and immunohistochemistry, respectively. Relationships between immunohistochemical scores and several clinicopathological variables were also examined.
  
  
  RESULTS
  Expression of NOB1 mRNA and protein in papillary thyroid carcinomatissue was significantly higher than in normal thyroid tissue and benign thyroid tissue, while there was no significant difference between normal thyroid tissue and benign thyroid tumour tissue. A high level of NOB1 protein expression was associated with large tumour size and Union for International Cancer Control stage, but was not significantly correlated with sex or age.
  
  
  CONCLUSIONS
  Compared with normal thyroid and benign thyroid tumour tissue, papillary thyroid carcinomas showed higher expression of NOB1, which indicates that the expression level of the NOB1 gene in the thyroid may play a key role in the occurrence and development of papillary thyroid carcinoma.
authors.affiliation: Department of Thyroid Surgery, The First Affiliated Hospital, Jilin University, Changchun, Jilin, PR China.
authors.forename: Shan
authors.initials: S
authors.lastname: Lin
authors.forename: Wei
authors.initials: W
authors.lastname: Meng
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.forename: Peisong
authors.initials: P
authors.lastname: Wang
authors.forename: Shuai
authors.initials: S
authors.lastname: Xue
authors.forename: Guang
authors.initials: G
authors.lastname: Chen
mesh_terms: D000328:Adult; D002277:Carcinoma / Q000235:genetics* / Q000473:pathology; D002291:Carcinoma, Papillary; D005260:Female; D015870:Gene Expression; D006044:Goiter, Nodular / Q000235:genetics* / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D009687:Nuclear Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D000077273:Thyroid Cancer, Papillary; D013961:Thyroid Gland / Q000378:metabolism / Q000473:pathology*; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000473:pathology; D047368:Tumor Burden
chemical_list: C506664:NOB1 protein, human; D009687:Nuclear Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins
keywords: NOB1; Papillary thyroid carcinoma; clinical significance; immunohistochemistry; quantitative RT–PCR