title: [Biodegradation of lignocellulose by Penicillium simplicissimum and characters of lignocellulolytic enzymes].
abstract: Penicillium simplicissimum(Oudem.) Thomrn BGA can secrete lignocellulolytic enzymes, among these enzymes the highest activities of hemicellulase, cellulase, lignin peroxidase (Lip), manganese peroxidase (Mnp) and laccase are 146.82 Iu.g-1, 2.78 U.g-1, 47.97 U.g-1, 34.56 U.g-1 and 17.94 U.g-1 respectively. According to the results and the statistical analysis of SPSS, the ability of secreting lignocellulolytic enzymes by Penicillium simplicissimum significantly correlated with the structure of lignocellulose, and the biodegradation of lignocellulose was probably a kind of synergistic effect of several lignocellulolytic enzymes. In the solid-state fermentation of 30 days, the hemicellulose content has a significantly negative correlation with the fermentation days(r = -0.946, P < 0.01), there was also a significantly negative correlation between the cellulose-biodegradation and the lignin-biodegradation (r = -0.818, P<0.05). As unselected enzymes, Lip and Mnp can degrade hemicellulose and cellulose corporately when biodegrades lignin. The significant correlation is showed between Lip, Mnp and cellulose (correlation parameters are r = 0. 922, P <0.01; r = 0.807, P<0.05 respectively). In addition, the biosorption is found to have a very important effect in the removal of liquid alkali lignin by Penicillium simplicissimum. Key words:Penicillium simplicissimum; lignocellulolytic enzymes; biodegradation; biosorption; synergistic effect
authors.affiliation: College of Environmental Science and Engineering, Hunan University, Changsha 410082, China. sy19000@163.com
authors.forename: Ying
authors.initials: Y
authors.lastname: Shen
authors.forename: Tian-Jue
authors.initials: TJ
authors.lastname: Hu
authors.forename: Guang-Ming
authors.initials: GM
authors.lastname: Zeng
authors.forename: Dan-Lian
authors.initials: DL
authors.lastname: Huang
authors.forename: Lu
authors.initials: L
authors.lastname: Yin
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Juan-Juan
authors.initials: JJ
authors.lastname: Wu
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
mesh_terms: D001673:Biodegradation, Environmental; D002480:Cellulase / Q000378:metabolism; D008031:Lignin / Q000378:metabolism*; D010407:Penicillium / Q000201:enzymology* / Q000378:metabolism*; D010544:Peroxidases / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism; D012037:Refuse Disposal / Q000379:methods*
chemical_list: D011134:Polysaccharides; C036909:lignocellulose; C007916:hemicellulose; D008031:Lignin; D010544:Peroxidases; C042858:lignin peroxidase; D002480:Cellulase