title: Measurement of inositol (1,4,5) trisphosphate in plant tissues by a competitive receptor binding assay.
abstract: The phosphoinositide signaling pathway is important for plant responses to many different stresses. As part of the responses to a stimulus, InsP3 levels may increase rapidly and transiently. The receptor binding assay for InsP3 described here is easy to use and an ideal method to monitor and compare InsP3 levels in multiple samples from large scale experiments. The method is based on competitive binding of InsP3 to the mammalian brain InsP3 specific receptor protein. This chapter describes a protocol for extracting and neutralizing plant samples and performing the receptor binding assay (using a commercially available kit). The protocol described has been used effectively to monitor InsP3 levels in plant tissues of different origin and in response to different stresses.
authors.affiliation: Department of Cellular Biochemistry, Institute for Biochemistry and Biotechnology, Martin-Luther-University Halle-Wittenberg, Halle, Germany.
authors.forename: Ingo
authors.initials: I
authors.lastname: Heilmann
authors.forename: Imara Y
authors.initials: IY
authors.lastname: Perera
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D001667:Binding, Competitive; D001681:Biological Assay / Q000379:methods*; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism*; D053496:Inositol 1,4,5-Trisphosphate Receptors / Q000378:metabolism; D009928:Organ Specificity; D011485:Protein Binding; D036226:Seedlings / Q000378:metabolism; D014316:Tritium
chemical_list: D053496:Inositol 1,4,5-Trisphosphate Receptors; D014316:Tritium; D015544:Inositol 1,4,5-Trisphosphate