title: F18ab Escherichia coli flagella expression is regulated by acyl-homoserine lactone and contributes to bacterial virulence.
abstract: To investigate the effect of the Quorum Sensing (QS)-I system on the expression of virulence factors in Shiga toxin producing and verotoxin-producing Escherichia coli (STEC and VTEC), the yenI gene from Yersinia enterocolitica was cloned into E. coli F18ab 107/86. Recombinant E. coli transformed with yenI produced acyl-homoserine lactone synthase (AHL), as measured using cross-streaking assays with the reporter biosensor strain Chromobacterium violaceum CV026. The AI-1 positive recombinant F18ab E. coli exhibited impaired expression of flagella, decreased motility, reduced biofilm formation and AI-2 production, as well as attenuated adherence and invasion on IPEC-J2 cells. This study provides new insights to the crucial function of AI-1 in regulating STEC virulence.
authors.affiliation: College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Fenghua
authors.initials: F
authors.lastname: Yao
authors.forename: Mingxu
authors.initials: M
authors.lastname: Zhou
authors.forename: Jun
authors.initials: J
authors.lastname: Zhu
authors.forename: Xinjun
authors.initials: X
authors.lastname: Zhang
authors.forename: Wenbin
authors.initials: W
authors.lastname: Bao
authors.forename: Shenglong
authors.initials: S
authors.lastname: Wu
authors.forename: Philip R
authors.initials: PR
authors.lastname: Hardwidge
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Zhu
mesh_terms: D054742:Acyl-Butyrolactones / Q000378:metabolism*; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D018441:Biofilms; D002460:Cell Line; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity*; D029968:Escherichia coli Proteins / Q000235:genetics; D036781:Fimbriae Proteins / Q000235:genetics; D005407:Flagella / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D053038:Quorum Sensing / Q000235:genetics; D013552:Swine; D015534:Trans-Activators / Q000235:genetics; D014774:Virulence / Q000235:genetics*
chemical_list: D054742:Acyl-Butyrolactones; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C113053:FedA protein, E coli; D015534:Trans-Activators; C096956:yenI protein, Yersinia enterocolitica; D036781:Fimbriae Proteins