title: Cytotoxicity evaluation of zinc oxide-eugenol and non-eugenol cements using different fibroblast cell lines.
abstract: OBJECTIVE
  Despite being commonly used as temporary cements in dentistry, there is a lack of studies regarding the cytotoxicity of zinc oxide-eugenol (ZOE) and zinc oxide non-eugenol (ZONE) cements. In addition, cytotoxicity evaluation of the materials often involves animal-based cells. Therefore, in this study, a cytotoxicity evaluation of commercially available ZOE and ZONE cements was carried out using both animal and human-based cells.
  
  
  METHODS
  The extraction or dilution of the extraction from four commercially available cements (two zinc oxide-eugenol and two zinc oxide non-eugenol) was tested for cytotoxicity, using three different cells and a water-soluble treatzolium salt assay. The results were confirmed using a confocal laser microscope following calcein AM and ethidium homodimer-1 staining.
  
  
  RESULTS
  The results showed that there was a significant difference in cell viability depending on which cell was used, even when the same material was tested. Generally, L929 showed relatively low cell viability with a low EC50 (effective concentration of extracts that caused 50% of cell viability compared to the control) value compared to both HGF-1 and hTERT-hNOF. Such results were also confirmed by a confocal laser microscope.
  
  
  CONCLUSIONS
  Careful consideration on interpreting the results for cytotoxicity evaluation of ZOE and ZONE cements is needed when different cells are used.
authors.affiliation: Research Center for Orofacial Hard Tissue Regeneration and Department and Research Institute of Dental Biomaterials and Bioengineering, Yonsei University College of Dentistry , Seoul , Republic of Korea.
authors.forename: Jae-Sung
authors.initials: JS
authors.lastname: Kwon
authors.forename: Rasika P
authors.initials: RP
authors.lastname: Illeperuma
authors.forename: Jin
authors.initials: J
authors.lastname: Kim
authors.forename: Kwang-Mahn
authors.initials: KM
authors.lastname: Kim
authors.forename: Kyoung-Nam
authors.initials: KN
authors.lastname: Kim
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects*; D003738:Dental Cements; D005054:Eugenol / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D051379:Mice; D015034:Zinc Oxide / Q000494:pharmacology*
chemical_list: D003738:Dental Cements; D005054:Eugenol; D015034:Zinc Oxide