title: Low expression of LOC285194 is associated with poor prognosis in colorectal cancer.
abstract: BACKGROUND
  The long non-coding RNAs (lncRNAs) study has gradually become one of the hot topics in the field of RNA biology. One lncRNA which has attracted attention is LOC285194, a lncRNA demonstrated the potential tumor-suppressor role in osteosarcoma. The aim of this study was to examine the expression of LOC285194 in colorectal cancer (CRC) patients and to investigate the relationship between this lncRNA levels and existing clinicopathologic parameters and patient survival.
  
  
  METHODS
  The expression of LOC285194 was detected by quantitative real-time polymerase chain reaction in pairs of tumorous and adjacent normal tissues of 81 colorectal cancer patients with a follow-up of 5 years, as well as in three colorectal cancer cell lines and normal intestinal mucous cell line. Then, we analyzed the potential relationship between this lncRNA levels in tumor tissues and existing clinicopathological features of CRC, and clinical outcome.
  
  
  RESULTS
  The relative expression levels of LOC285194 was significantly lower in tumor tissues (p<0.001) and colorectal cancer cell lines compared with adjacent normal tissues and normal intestinal mucous cell line. In addition, low expression of LOC285194 was correlated with larger tumor size (p=0.015), higher tumor stage (p=0.034), and more distant metastasis (p=0.046). Kaplan-Meier analysis indicated that patients with low LOC285194 expression had a poor disease free survival (p=0.010). Moreover, multivariate analysis showed that decreased expression of LOC285194 was an independent predictor of disease-specific survival.
  
  
  CONCLUSIONS
  Our data indicate that LOC285194 might be a novel prognostic indicator in colorectal cancer and may be a potential target for diagnosis and gene therapy.
authors.forename: Peng
authors.initials: P
authors.lastname: Qi
authors.forename: Mi-die
authors.initials: MD
authors.lastname: Xu
authors.forename: Shu-juan
authors.initials: SJ
authors.lastname: Ni
authors.forename: Dan
authors.initials: D
authors.lastname: Huang
authors.forename: Ping
authors.initials: P
authors.lastname: Wei
authors.forename: Cong
authors.initials: C
authors.lastname: Tan
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Zhou
authors.forename: Xiang
authors.initials: X
authors.lastname: Du
mesh_terms: D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D011379:Prognosis; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D062085:RNA, Long Noncoding; C581996:loc285194 LncRNA, human