title: Quality of harvest and role of cell dose in unrelated bone marrow transplantation: an Italian Bone Marrow Donor Registry-Gruppo Italiano Trapianto di Midollo Osseo Study.
abstract: In this study, we investigated the factors affecting cell dose harvest and the role of cell dose on outcome. We analysed data from a cohort of 703 patients who underwent unrelated bone marrow transplantation facilitated by IBMDR in GITMO centers between 2002 and 2008. The median-infused cell doses is 3.7 × 10(8)/kg, the correlation between the nucleated cells requested from transplant centers and those harvested by collection centers was adequate. A harvested/requested cells ratio lower than 0.5 was observed only in 3% of harvests. A volume of harvested marrow higher than the median value of 1270 ml was related to a significant lower infused cell dose (χ(2): 44.4; P < 0.001). No patient- or donor-related variables significantly influenced the cell dose except for the recipient younger age (χ(2): 95.7; P < 0.001) and non-malignant diseases (χ(2): 33.8; P < 0.001). The cell dose resulted an independent predictor factor for a better outcome in patients affected by non-malignant disease (P = 0.05) while early disease malignant patients receiving a lower cell dose showed a higher risk of relapse (P = 0.05).
authors.forename: Franca
authors.initials: F
authors.lastname: Fagioli
authors.forename: Paola
authors.initials: P
authors.lastname: Quarello
authors.forename: Simona
authors.initials: S
authors.lastname: Pollichieni
authors.forename: Teresa
authors.initials: T
authors.lastname: Lamparelli
authors.forename: Massimo
authors.initials: M
authors.lastname: Berger
authors.forename: Fabio
authors.initials: F
authors.lastname: Benedetti
authors.forename: Veronica
authors.initials: V
authors.lastname: Barat
authors.forename: Renato
authors.initials: R
authors.lastname: Marciano
authors.forename: Alessandro
authors.initials: A
authors.lastname: Rambaldi
authors.forename: Andrea
authors.initials: A
authors.lastname: Bacigalupo
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Sacchi
mesh_terms: D000293:Adolescent; D000328:Adult; D016026:Bone Marrow Transplantation / Q000009:adverse effects / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007558:Italy; D008297:Male; D008875:Middle Aged; D012008:Recurrence; D020858:Tissue and Organ Harvesting / Q000379:methods* / Q000592:standards; D019172:Transplantation Conditioning / Q000009:adverse effects / Q000379:methods; D014184:Transplantation, Homologous; D016896:Treatment Outcome; D061349:Unrelated Donors; D055815:Young Adult
keywords: Cell dose; Harvest; Relapse; Unrelated bone marrow transplantation