title: Comparative analysis of the chloroplast proteomes of a wheat (Triticum aestivum L.) single seed descent line and its parents.
abstract: To understand the photosynthetic basis in a single seed descent line 10 (SSDL10) of wheat contained high ATP in leaves, the chloroplast proteome was compared to SSDL10 and its parents using a combination of 2-DE and MALDI-TOF MS and MS/MS. More than 300 protein spots could be reproducibly detected in the 2D gel. 18 spots were differentially expressed between SSDL10 and the parents, 16 of which were identified by MS with the localization in chloroplasts. These proteins are grouped into diverse functional categories, including Calvin cycle and electron transport in photosynthesis, redox homeostasis, metabolism, and regulation. In addition to Rubisco large subunit, the content of photosynthetic electron transfers such as chlorophyll a-b binding protein, ATP synthase δ subunit, ferredoxin-NADP⁺ oxidoreductase (FNR) was higher in SSDL10 than in its parents. Furthermore, cyclic electron transfer around photosystem I (CET) was faster in SSDL10 than in the parents. Analysis of NADPH-NBT oxidoreductase activity combined with immuno-detection further revealed that, the activity of two high molecular mass protein complexes containing FNR probably involved, the CET appeared higher in SSDL10 than in the parents. The possible mechanism for the regulative role of CET in photosynthesis in SSDL10 is discussed.
authors.affiliation: National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China.
authors.forename: Zhi-Hui
authors.initials: ZH
authors.lastname: He
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Li
authors.forename: Yunkang
authors.initials: Y
authors.lastname: Shen
authors.forename: Zhen-Sheng
authors.initials: ZS
authors.lastname: Li
authors.forename: Hualing
authors.initials: H
authors.lastname: Mi
mesh_terms: D002736:Chloroplasts / Q000378:metabolism*; D004579:Electron Transport; D010788:Photosynthesis; D045331:Photosystem I Protein Complex / Q000378:metabolism*; D018515:Plant Leaves / Q000378:metabolism; D020543:Proteome / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D014908:Triticum / Q000235:genetics / Q000378:metabolism*
chemical_list: D045331:Photosystem I Protein Complex; D020543:Proteome
keywords: 2-cys Prx; 2-cysteine peroxiredoxins; ATP synthase; ATPase; CET; Chloroplast; Cyclic electron transfer; Cytb(6)f; FNR; FQR; GS; MALDI TOF; MS/MS; NAD(P)H dehydrogenase; NDH; OEC; OEE1; P700; PSI; PSII; Photosynthesis; Proteomics; RCA; Rubisco; TRX m; Wheat (Triticum aestivum L.); cyclic electron transfer; cytochrome b(6)f; ferredoxin-NADP(+) oxidoreductase; ferredoxin-plastoquinone reductase; glutamine synthase; m-type thioredoxin; matrix-assisted laser desorption/ionization time of flight; oxygen-evolving complex; oxygen-evolving enhancer 1; photosystem I; photosystem II; ribulose-1,5-bisphosphate carboxylase/oxygenase; rubisco activase; tandem mass spectroscopy; the reaction center chlorophylls in PSI