title: Purification, characterisation and expression in Saccharomyces cerevisiae of LipG7 an enantioselective, cold-adapted lipase from the Antarctic filamentous fungus Geomyces sp. P7 with unusual thermostability characteristics.
abstract: A lipase, LipG7, has been purified from the Antarctic filamentous fungus Geomyces sp. P7 which was found to be cold-adapted and able to retain/regain its activity after heat denaturation. The LipG7 exhibits 100% residual activity following 1h incubation at 100°C whilst simultaneously showing kinetic adaptations to cold temperatures. LipG7 was also found to have industrial potential as an enantioselective biocatalyst as it is able to effectively catalyse the enantioselective transesterification of a secondary alcohol. The LipG7 coding sequence has been identified and cloned using 454 pyrosequencing of the transcriptome and inverse PCR. The LipG7 protein has been heterologously expressed in Saccharomyces cerevisiae BJ5465 and shown to exhibit the same characteristics as the native protein.
authors.affiliation: Faculty of Biotechnology and Food Sciences, Institute of Technical Biochemistry, Technical University of Lodz, ul. B. Stefanowskiego 4/10, 90-924 Lodz, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Florczak
authors.forename: Maurycy
authors.initials: M
authors.lastname: Daroch
authors.forename: Mark Charles
authors.initials: MC
authors.lastname: Wilkinson
authors.forename: Aneta
authors.initials: A
authors.lastname: Białkowska
authors.forename: Andrew Derek
authors.initials: AD
authors.lastname: Bates
authors.forename: Marianna
authors.initials: M
authors.lastname: Turkiewicz
authors.forename: Lesley Ann
authors.initials: LA
authors.lastname: Iwanejko
mesh_terms: D000222:Adaptation, Physiological; D000864:Antarctic Regions; D001203:Ascomycota / Q000201:enzymology* / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D001709:Biotechnology / Q000379:methods; D003001:Cloning, Molecular; D003080:Cold Temperature; D004795:Enzyme Stability; D005658:Fungi / Q000201:enzymology* / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008049:Lipase / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D013237:Stereoisomerism
chemical_list: D001426:Bacterial Proteins; D008049:Lipase