title: A novel spectrophotometric method for the determination oxacillin sodium.
abstract: A simple and sensitive spectrophotometric method was developed for the determination of Oxacillin sodium. The method was based on charge transfer complexation reaction of the drug with iodine in methanol - dichloromethane medium. The absorbance was measured at 365 nm against the reagent blank. Under optimized experimental conditions, Beer's law is obeyed in the concentration ranges 2-8 μg/ml for Oxacillin Sodium. The method was validated for specificity, linearity, precision, accuracy, limit of quantitation, robustness and ruggedness. The LOD and LOQ value were 0.39 and 1.18 μg/ml respectively. The method was successfully applied to the analysis of Oxacillin sodium in Human Urine samples with good accuracy and precision.
authors.affiliation: Vardhman Chemtech Ltd, Nimbua, Dera Bassi, Mohali, Punjab, India.
authors.forename: Rajinder Singh
authors.initials: RS
authors.lastname: Gujral
authors.forename: Sk Manirul
authors.initials: SM
authors.lastname: Haque
authors.forename: Prem
authors.initials: P
authors.lastname: Shanker
keywords: Oxacillin sodium; human urine; iodine; spectrophotometer; validation