title: Identification of aldolase and ferredoxin reductase within the dbt operon of Burkholderia fungorum DBT1.
abstract: Burkholderia fungorum DBT1, first isolated from settling particulate matter of an oil refinery wastewater, is a bacterial strain which has been shown capable of utilizing several polycyclic aromatic hydrocarbons (PAHs) including dibenzothiophene (DBT). In particular, this microbe is able to efficiently degrade DBT through the Kodama pathway. Previous investigations have lead to the identification of six genes, on a total of eight, required for DBT degradation. In the present study, a combined experimental/computational approach was adopted to identify and in silico characterize the two missing genes, namely a ferredoxin reductase and a hydratase-aldolase. Thus, the finding of all enzymatic components of the Kodama pathway in B. fungorum DBT1 makes this bacterial strain amenable for possible exploitation in soil bioremediation protocols.
authors.affiliation: Department of Biotechnology, University of Verona, Strada Le Grazie 15, 37134, Verona, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Piccoli
authors.forename: Marco
authors.initials: M
authors.lastname: Andreolli
authors.forename: Alejandro
authors.initials: A
authors.lastname: Giorgetti
authors.forename: Fabio
authors.initials: F
authors.lastname: Zordan
authors.forename: Silvia
authors.initials: S
authors.lastname: Lampis
authors.forename: Giovanni
authors.initials: G
authors.lastname: Vallini
mesh_terms: D001711:Biotransformation; D019117:Burkholderia / Q000201:enzymology* / Q000235:genetics; D005287:Ferredoxin-NADP Reductase / Q000235:genetics* / Q000378:metabolism*; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics* / Q000378:metabolism*; D053858:Metabolic Networks and Pathways; D009876:Operon; D012989:Soil Pollutants / Q000378:metabolism; D013876:Thiophenes / Q000378:metabolism
chemical_list: D012989:Soil Pollutants; D013876:Thiophenes; D005287:Ferredoxin-NADP Reductase; D005634:Fructose-Bisphosphate Aldolase; C016366:dibenzothiophene
keywords: Burkholderia fungorum DBT1; Dibenzothiophene; Polycyclic aromatic hydrocarbons; Protein structure bioinformatics; Targeting gene walking