title: RNA interference mediated pten knock-down inhibit the formation of polycystic ovary.
abstract: Pten (phosphatase and tensin homolog deleted on chromosome 10), a kind of tumor suppressor gene, plays important roles in female reproductive system. But its expression and roles in the formation of polycystic ovaries are yet to be known. In this study, we constructed a rat model of PCOS using norethindrone and HCG injections and found the expressions of pten mRNA and PTEN protein increased significantly in the polycystic ovary tissue by immunohistochemistry, RT-PCR, and western blot. Furthermore, the results showed that in vivo ovaries could be effectively transfected by lentiviral vectors through the ovarian microinjection method and indicated that pten shRNA may inhibit the formation of polycystic ovaries by pten down-regulation. Our study provides new information regarding the role of PTEN in female reproductive disorders, such as polycystic ovary syndrome.
authors.affiliation: Medical Experimental Teaching Department, Nanchang University, Nanchang 330031, China.
authors.forename: Jie-Xiu
authors.initials: JX
authors.lastname: Ouyang
authors.forename: Tao
authors.initials: T
authors.lastname: Luo
authors.forename: Hui-Yun
authors.initials: HY
authors.lastname: Sun
authors.forename: Jian
authors.initials: J
authors.lastname: Huang
authors.forename: Dan-Feng
authors.initials: DF
authors.lastname: Tang
authors.forename: Lei
authors.initials: L
authors.lastname: Wu
authors.forename: Yue-Hui
authors.initials: YH
authors.lastname: Zheng
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Zheng
mesh_terms: D000818:Animals; D015153:Blotting, Western; D004195:Disease Models, Animal; D004958:Estradiol / Q000097:blood; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D006801:Humans; D007150:Immunohistochemistry; D007986:Luteinizing Hormone / Q000097:blood; D010053:Ovary / Q000378:metabolism* / Q000473:pathology; D051059:PTEN Phosphohydrolase / Q000235:genetics* / Q000378:metabolism; D011085:Polycystic Ovary Syndrome / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013739:Testosterone / Q000097:blood
chemical_list: D013739:Testosterone; D004958:Estradiol; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone; D051059:PTEN Phosphohydrolase