title: Enhancement of supercritical CO2 inactivation of spores of Penicillium oxalicum by ethanol cosolvent.
abstract: The inactivation of spores of Penicillium oxalicum by supercritical carbon dioxide (SC-CO2) was optimized by response surface methodology. The optimal inactivation conditions of 16.8 MPa, 49°C, and 20 min were determined using ridge analysis, at which the predicted and experimental log10 reductions were obtained as 5.74 and 6.12, respectively. The synergistic effect of a cosolvent (ethanol), which was used to modify SC-CO2, on the inactivation of the fungal spores was investigated. At less severe conditions of 10 MPa and 40°C, P. oxalicum spores of 10(7) CFU/ml were completely inactivated within 45 min by SC-CO2 modified with ethanol.
authors.affiliation: School of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Korea.
authors.forename: Hyong Seok
authors.initials: HS
authors.lastname: Park
authors.forename: Kyoung Heon
authors.initials: KH
authors.lastname: Kim
mesh_terms: D002245:Carbon Dioxide / Q000494:pharmacology*; D015169:Colony Count, Microbial; D004202:Disinfectants / Q000494:pharmacology*; D000431:Ethanol / Q000494:pharmacology*; D006874:Hydrostatic Pressure; D050296:Microbial Viability / Q000187:drug effects; D010407:Penicillium / Q000187:drug effects*; D013172:Spores, Fungal / Q000187:drug effects*; D013696:Temperature; D013997:Time Factors
chemical_list: D004202:Disinfectants; D002245:Carbon Dioxide; D000431:Ethanol