title: Nanocomposite hydrogels for cartilage tissue engineering: mesoporous silica nanofibers interlinked with siloxane derived polysaccharide.
abstract: Injectable materials for mini-invasive surgery of cartilage are synthesized and thoroughly studied. The concept of these hybrid materials is based on providing high enough mechanical performances along with a good medium for chondrocytes proliferation. The unusual nanocomposite hydrogels presented herein are based on siloxane derived hydroxypropylmethylcellulose (Si-HPMC) interlinked with mesoporous silica nanofibers. The mandatory homogeneity of the nanocomposites is checked by fluorescent methods, which show that the silica nanofibres dispersion is realized down to nanometric scale, suggesting an efficient immobilization of the silica nanofibres onto the Si-HPMC scaffold. Such dispersion and immobilization are reached thanks to the chemical affinity between the hydrophilic silica nanofibers and the pendant silanolate groups of the Si-HPMC chains. Tuning the amount of nanocharges allows tuning the resulting mechanical features of these injectable biocompatible hybrid hydrogels. hASC stem cells and SW1353 chondrocytic cells viability is checked within the nanocomposite hydrogels up to 3 wt% of silica nanofibers.
authors.affiliation: Institut des Matériaux Jean Rouxel (IMN), CNRS UMR 6502, Université de Nantes, 2 rue de la Houssinière, B.P. 32229, 44322, Nantes Cedex 3, France.
authors.forename: Nela
authors.initials: N
authors.lastname: Buchtová
authors.forename: Gildas
authors.initials: G
authors.lastname: Réthoré
authors.forename: Cécile
authors.initials: C
authors.lastname: Boyer
authors.forename: Jérôme
authors.initials: J
authors.lastname: Guicheux
authors.forename: Frédéric
authors.initials: F
authors.lastname: Rambaud
authors.forename: Karine
authors.initials: K
authors.lastname: Vallé
authors.forename: Philippe
authors.initials: P
authors.lastname: Belleville
authors.forename: Clément
authors.initials: C
authors.lastname: Sanchez
authors.forename: Olivier
authors.initials: O
authors.lastname: Chauvet
authors.forename: Pierre
authors.initials: P
authors.lastname: Weiss
authors.forename: Jean
authors.initials: J
authors.lastname: Le Bideau
mesh_terms: D002356:Cartilage / Q000166:cytology / Q000502:physiology; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003432:Cross-Linking Reagents / Q000138:chemical synthesis / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry* / Q000494:pharmacology; D008422:Materials Testing; D053761:Nanocomposites / Q000737:chemistry; D057139:Nanofibers / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D012833:Siloxanes / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D003432:Cross-Linking Reagents; D020100:Hydrogels; D011134:Polysaccharides; D012833:Siloxanes; D012822:Silicon Dioxide