title: Replication of white spot syndrome virus (WSSV) in the polychaete Dendronereis spp.
abstract: This study investigated whether WSSV replicates in naturally infected Dendronereis spp., a common polychaete (Nereididae) species in shrimp ponds in Indonesia. To detect WSSV replication, (i) immunohistochemistry (IHC) using a monoclonal antibody against WSSV VP28 protein and (ii) nested RT-PCR using specific primers set for the vp28 gene to detect WSSV-specific mRNA were applied. WSSV immunoreactive-nuclei were detected in the gut epithelium of the polychaete and WSSV mRNA was detected with nested RT-PCR. This, together with the IHC results, confirmed that WSSV could replicate in Dendronereis spp. This is the first report showing that WSSV replicated in a naturally infected non-crustacean host.
authors.affiliation: Aquaculture and Fisheries Group, Wageningen University, The Netherlands; Fisheries Department, Faculty of Fisheries and Marine Sciences, Diponegoro University, Semarang, Indonesia. rinadesrina@yahoo.com
authors.lastname: Desrina
authors.forename: J A J
authors.initials: JA
authors.lastname: Verreth
authors.forename: S B
authors.initials: SB
authors.lastname: Prayitno
authors.forename: J H W M
authors.initials: JH
authors.lastname: Rombout
authors.forename: J M
authors.initials: JM
authors.lastname: Vlak
authors.forename: M C J
authors.initials: MC
authors.lastname: Verdegem
mesh_terms: D000818:Animals; D054884:Host-Pathogen Interactions; D011077:Polychaeta / Q000821:virology*; D012333:RNA, Messenger; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014779:Virus Replication; D046848:White spot syndrome virus 1 / Q000235:genetics / Q000502:physiology*
chemical_list: D012333:RNA, Messenger
keywords: Dendronereis spp.; Immunohistochemistry; RT-PCR; Replication; WSSV