title: Myofilament lattice structure in presence of a skeletal myopathy-related tropomyosin mutation.
abstract: Human tropomyosin mutations deregulate skeletal muscle contraction at the cellular level. One key feature is the slowing of the kinetics of force development. The aim of the present study was to characterize the potential underlying molecular mechanisms by recording and analyzing the X-ray diffraction patterns of human membrane-permeabilized muscle cells expressing a particular β-tropomyosin mutation (E41K). During resting conditions, the d1,0 lattice spacing, Δ1,0 and I1,1 to I1,0 ratio were not different from control values. These results suggest that, in presence of the E41K β-tropomyosin mutation, the myofilament lattice geometry is well maintained and therefore may not have any detrimental influence on the contraction mechanisms and thus, on the rate of force generation.
authors.affiliation: Centre of Human & Aerospace Physiological Sciences, King's College London, Room 3.3, Shepherd's House, Guy's Campus, London, SE1 1UL, UK, julien.ochala@kcl.ac.uk.
authors.forename: Julien
authors.initials: J
authors.lastname: Ochala
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Iwamoto
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D018482:Muscle, Skeletal / Q000378:metabolism / Q000473:pathology*; D009154:Mutation; D009210:Myofibrils / Q000235:genetics* / Q000378:metabolism; D017696:Myopathies, Nemaline / Q000235:genetics* / Q000378:metabolism; D014335:Tropomyosin / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014961:X-Ray Diffraction
chemical_list: D014335:Tropomyosin