title: Neurotrophins play differential roles in short and long-term recognition memory.
abstract: The neurotrophin family of proteins are believed to mediate various forms of synaptic plasticity in the adult brain. Here we have assessed the roles of these proteins in object recognition memory in the rat, using icv infusions of function-blocking antibodies or the tyrosine kinase antagonist, tyrphostin AG879, to block Trk receptors. We report that tyrphostin AG879 impairs both short-term and long-term recognition memory, indicating a requirement for Trk receptor activation in both processes. The effect of inhibition of each of the neurotrophins with activity-blocking neutralising antibodies was also tested. Treatment with anti-BDNF, anti-NGF or anti-NT4 had no effect on short-term memory, but blocked long-term recognition memory. Treatment with anti-NT3 had no effect on either process. We also assessed changes in expression of neurotrophins and their respective receptors in the hippocampus, dentate gyrus and perirhinal cortex over a 24 h period following training in the object recognition task. We observed time-dependent changes in expression of the Trk receptors and their ligands in the dentate gyrus and perirhinal cortex. The data are consistent with a pivotal role for neurotrophic factors in the expression of recognition memory.
authors.affiliation: Department of Physiology, School of Medicine, and Trinity College Institute of Neuroscience, University of Dublin, Trinity College, Dublin 2, Ireland. callaghc@tcd.ie
authors.forename: Charlotte K
authors.initials: CK
authors.lastname: Callaghan
authors.forename: Aine M
authors.initials: AM
authors.lastname: Kelly
mesh_terms: D000818:Animals; D019138:Antibodies, Blocking / Q000494:pharmacology; D019208:Brain-Derived Neurotrophic Factor / Q000037:antagonists & inhibitors / Q000378:metabolism; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism; D008297:Male; D057567:Memory, Long-Term / Q000187:drug effects / Q000502:physiology*; D008570:Memory, Short-Term / Q000187:drug effects / Q000502:physiology*; D020932:Nerve Growth Factor / Q000037:antagonists & inhibitors / Q000378:metabolism; D009414:Nerve Growth Factors / Q000037:antagonists & inhibitors / Q000187:drug effects / Q000378:metabolism*; D020933:Neurotrophin 3 / Q000037:antagonists & inhibitors / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D020794:Receptor Protein-Tyrosine Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D021641:Recognition, Psychology; D020032:Tyrphostins / Q000494:pharmacology
chemical_list: C081020:AG-879; D019138:Antibodies, Blocking; D019208:Brain-Derived Neurotrophic Factor; D009414:Nerve Growth Factors; D020933:Neurotrophin 3; D020032:Tyrphostins; D020932:Nerve Growth Factor; D020794:Receptor Protein-Tyrosine Kinases; C073922:neurotrophin 4
keywords: BDNF; Dentate gyrus; Learning; Memory; Neurotrophin; Perirhinal cortex