title: [In situ genotoxity evaluation of triazines using Tradescantia clone 4430 Trad-SHM bioassay].
abstract: Tradescantia 4430 clone stamen hair mutation (Trad-SHM) bioassay was used to evaluate the genotoxicity of a herbicide composed of triazines (atrazine and simazine) after in situ exposure. Thirty plant pots were exposed during the herbicide application (test group) and a control group was maintained in a greenhouse (control group). Genotoxicity was expressed in terms of pink mutation events (EMPs) and the data analysis was performed by Student's t test comparing the control and contaminated group after eight-days (C8D = 8-day control; T8D = 8-day test) and peak day (CPD = peak day control; TPD = peak day test). Exposure to the herbicide caused a significantly higher number of EMPs in the test group (T8D = 2.27; TPD = 4.69) than in the control group (C8D = 0.71; CPD = 0.62). This demonstrates that the Trad-SHM bioassay is sensitive and efficient and can be used as tool to assess the genotoxic potential of environmental contaminants like triazines associated with adverse effects on human health.
authors.affiliation: Universidade do Oeste de Santa Catarina, SC, 89600-000. caripatussi@yahoo.com.br
authors.forename: Carina
authors.initials: C
authors.lastname: Patussi
authors.forename: Márcia
authors.initials: M
authors.lastname: Bündchen
mesh_terms: D001681:Biological Assay / Q000379:methods*; D009152:Mutagenicity Tests / Q000379:methods*; D009154:Mutation; D034823:Tradescantia / Q000187:drug effects*; D014227:Triazines / Q000633:toxicity*
chemical_list: D014227:Triazines