title: Overexpression of the urokinase receptor splice variant uPAR-del4/5 in breast cancer cells affects cell adhesion and invasion in a dose-dependent manner and modulates transcription of tumor-associated genes.
abstract: mRNA levels of the urokinase receptor splice variant uPAR-del4/5 are associated with prognosis in breast cancer. Its overexpression in cancer cells affects tumor biologically relevant processes. In the present study, individual breast cancer cell clones displaying low vs. high uPAR-del4/5 expression were analyzed demonstrating that uPAR-del4/5 leads to reduced cell adhesion and invasion in a dose-dependent manner. Additionally,matrix metalloproteinase-9 (MMP-9) was found to be strongly upregulated in uPAR-del4/5 overexpressing compared to vector control cells. uPAR-del4/5 may thus play an important role in the regulation of the extracellular proteolytic network and, by this, influence the metastatic potential of breast cancer cells.
authors.affiliation: Clinical Research Unit , Department of Obstetrics and Gynecology, Technical University of Munich, Ismaninger Str. 22, D-81675 Munich, Germany
authors.forename: Bettina
authors.initials: B
authors.lastname: Grismayer
authors.forename: Sumito
authors.initials: S
authors.lastname: Sato
authors.forename: Charlotte
authors.initials: C
authors.lastname: Kopitz
authors.forename: Christian
authors.initials: C
authors.lastname: Ries
authors.forename: Susanne
authors.initials: S
authors.lastname: Soelch
authors.forename: Manfred
authors.initials: M
authors.lastname: Schmitt
authors.forename: Gustavo
authors.initials: G
authors.lastname: Baretton
authors.forename: Achim
authors.initials: A
authors.lastname: Krüger
authors.forename: Thomas
authors.initials: T
authors.lastname: Luther
authors.forename: Matthias
authors.initials: M
authors.lastname: Kotzsch
authors.forename: Viktor
authors.initials: V
authors.lastname: Magdolen
mesh_terms: D001940:Breast / Q000166:cytology / Q000378:metabolism* / Q000473:pathology; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000235:genetics; D009361:Neoplasm Invasiveness / Q000235:genetics; D012326:RNA Splicing; D055293:Receptors, Urokinase Plasminogen Activator / Q000235:genetics*; D015854:Up-Regulation
chemical_list: D055293:Receptors, Urokinase Plasminogen Activator; D020780:Matrix Metalloproteinase 9