title: Daidzein inhibits carbohydrate digestive enzymes in vitro and alleviates postprandial hyperglycemia in diabetic mice.
abstract: This study was designed to investigate whether daidzein inhibits α-glucosidase and α-amylase activities and alleviates postprandial hyperglycemia in streptozotocin-induced diabetic mice. Daidzein showed prominent inhibitory effects against α-glucosidase and α-amylase. The IC50 values of daidzein against α-glucosidase and α-amylase were 0.048 and 0.301 mmol, respectively, which showed that daidzein was more effective than acarbose. The increase in postprandial blood glucose levels was more significantly suppressed in the daidzein-administered group than in the water group of both streptozotocin-induced diabetic and normal mice. Moreover, the area under the curve was significantly lowered following daidzein administration (2043 versus 2475 mmol min l) in the streptozotocin-induced diabetic mice. These results indicated that daidzein may be a potent α-glucosidase inhibitor and suppress the postprandial hyperglycemia caused by starch.
authors.affiliation: Department of Food Science and Nutrition, Pusan National University, Busan 609-735, Republic of Korea.
authors.forename: Mi-Hwa
authors.initials: MH
authors.lastname: Park
authors.forename: Jae-Won
authors.initials: JW
authors.lastname: Ju
authors.forename: Min Jung
authors.initials: MJ
authors.lastname: Park
authors.forename: Ji Sook
authors.initials: JS
authors.lastname: Han
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D050260:Carbohydrate Metabolism / Q000187:drug effects*; D048909:Diabetes Complications / Q000097:blood / Q000188:drug therapy* / Q000201:enzymology; D004791:Enzyme Inhibitors / Q000494:pharmacology / Q000627:therapeutic use; D065089:Glycoside Hydrolase Inhibitors; D006943:Hyperglycemia / Q000097:blood / Q000188:drug therapy* / Q000201:enzymology; D007529:Isoflavones / Q000494:pharmacology* / Q000627:therapeutic use; D008297:Male; D051379:Mice; D000516:alpha-Amylases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D007529:Isoflavones; C004742:daidzein; D000516:alpha-Amylases; D000520:alpha-Glucosidases