title: Effect of zinc supplementation on serum antibody titers to heat shock protein 27 in patients with thalassemia major.
abstract: Objective This current study was conducted to determine the effect of zinc supplementation on antibody titers to heat shock protein 27 (anti-HSP27) in patients with beta-thalassemia major (β-TM). Methods This was a double-blinded placebo-controlled clinical trial conducted at Dr Sheikh Hospital (Mashhad, Iran) from 2011 to 2012. Sixty-four patients (41 females and 23 males), aged between 8 and 18 years with transfusion-dependent β-TM were randomly allocated to two age- and sex-matched groups. The zinc (case) group received 30 mg of daily zinc sulfate supplementation and the placebo (control) group received same shape and color placebo over 9 months period of the trial. Serum anti-HSP27 titers were measured at the third and ninth months of the trial, using an in-house enzyme-linked immune-absorbent assay. Result There was a significant difference in anti-HSP27 titers, between the groups after 9 months. The baseline value of anti-HSP27 was 0.44 ± 0.15 in zinc group and were significantly decreased to 0.40 ± 0.18 after 9 months on treatment, while the baseline value of anti-HSP27 were significantly increased from 0.43 ± 0.17 to 0.44 ± 0.18 in the placebo group (P = 0.01). Conclusion Serum anti-HSP27 titers were significantly reduced in patients with β-TM treated with zinc supplements compared to a group treated with a placebo. It suggests that the potential antioxidant and anti-inflammatory effects of zinc supplements may account for a reduction in anti-HSP27 titers in patients with β-TM.
authors.forename: Elham
authors.initials: E
authors.lastname: Ghahramanlu
authors.forename: Abdollah
authors.initials: A
authors.lastname: Banihashem
authors.forename: Naghme-Zahra
authors.initials: NZ
authors.lastname: Mirhossini
authors.forename: Golkoo
authors.initials: G
authors.lastname: Hosseini
authors.forename: Hesam
authors.initials: H
authors.lastname: Mostafavi-Toroghi
authors.forename: Shima
authors.initials: S
authors.lastname: Tavallaie
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Meshkat
authors.forename: Majid
authors.initials: M
authors.lastname: Ghayour-Mobarhan
authors.forename: Gordon
authors.initials: G
authors.lastname: Ferns
mesh_terms: D000293:Adolescent; D002648:Child; D019587:Dietary Supplements; D004311:Double-Blind Method; D005260:Female; D055551:HSP27 Heat-Shock Proteins / Q000097:blood*; D006360:Heat-Shock Proteins; D006801:Humans; D008297:Male; D018832:Molecular Chaperones; D015032:Zinc / Q000008:administration & dosage*; D017086:beta-Thalassemia / Q000097:blood* / Q000188:drug therapy*
chemical_list: D055551:HSP27 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D015032:Zinc
keywords: HSP27 Heat-Shock Proteins; Thalassemia; Zinc