title: Detection and identification of cancerous murine fibroblasts, transformed by murine sarcoma virus in culture, using Raman spectroscopy and advanced statistical methods.
abstract: BACKGROUND
  Cancer is one of the leading worldwide causes of death. It may be induced by a variety of factors, including carcinogens, radiation, genetic factors, or DNA and RNA viruses. The early detection of cancer is critical for its successful therapy, which can result in complete recovery from some types of cancer.
  
  
  METHODS
  Raman spectroscopy has been widely used in medicine and biology. It is a noninvasive, nondestructive, and water-insensitive technique that can detect changes in cells and tissues that are caused by different disorders, such as cancer. In this study, Raman spectroscopy was used for the identification and characterization of murine fibroblast cell lines (NIH/3T3) and malignant fibroblast cells transformed by murine sarcoma virus (NIH-MuSV) cells.
  
  
  RESULTS
  Using principal component analysis and LDA it was possible to differentiate between the NIH/3T3 and NIH-MuSV cells with an 80-85% success rate based on their Raman shift spectra.
  
  
  CONCLUSIONS
  The best results for differentiation were achieved from spectra that were obtained from the rich membrane sites.
  
  
  CONCLUSIONS
  Because of its homogeneity and complete control of most factors affecting its growth, cell culture is a preferred model for the detection and identification of specific biomarkers related to cancer transformation or other cellular modifications.
authors.affiliation: Department of Physics, SCE - ShamoonCollege of Engineering, Beer-Sheva 84100, Israel. ahmad@sce.ac.il
authors.forename: A
authors.initials: A
authors.lastname: Salman
authors.forename: E
authors.initials: E
authors.lastname: Shufan
authors.forename: L
authors.initials: L
authors.lastname: Zeiri
authors.forename: M
authors.initials: M
authors.lastname: Huleihel
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000473:pathology* / Q000821:virology; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D002472:Cell Transformation, Viral; D003588:Cytopathogenic Effect, Viral; D005347:Fibroblasts / Q000473:pathology* / Q000821:virology; D051379:Mice; D025202:Molecular Diagnostic Techniques; D008980:Moloney murine sarcoma virus / Q000502:physiology; D041681:NIH 3T3 Cells; D025341:Principal Component Analysis; D013059:Spectrum Analysis, Raman