title: HLA-G 3' untranslated region polymorphisms are associated with systemic lupus erythematosus in 2 Brazilian populations.
abstract: OBJECTIVE
  HLA-G has well recognized tolerogenic properties in physiological and nonphysiological conditions. The 3' untranslated region (3'UTR) of the HLA-G gene has at least 3 polymorphic sites (14-bpINS/DEL, +3142C/G, and +3196C/G) described as associated with posttranscriptional influence on messenger RNA production; however, only the 14-bpINS/DEL and +3142C/G sites have been studied in systemic lupus erythematosus (SLE).
  
  
  METHODS
  We investigated the HLA-G 3'UTR polymorphic sites (14-bpINS/DEL, +3003C/T, +3010C/G, +3027A/C, +3035C/T, +3142C/G, +3187A/G, and +3196C/G) in 190 Brazilian patients with SLE and 282 healthy individuals in allele, genotype, and haplotype analyses. A multiple logistic regression model was used to assess the association of the disease features with the HLA-G 3'UTR haplotypes.
  
  
  RESULTS
  Increased frequencies were observed of the 14-bpINS (p = 0.053), +3010C (p = 0.008), +3142G (p = 0.006), and +3187A (p = 0.013) alleles, and increased frequencies of the 14-bpINS-INS (p = 0.094), +3010 C-C (p = 0.033), +3142 G-G (p = 0.021), and +3187 A-A (p = 0.035) genotypes. After Bonferroni correction, only the +3142G (p = 0.05) and +3010C (p = 0.06) alleles were overrepresented in SLE patients. The UTR-1 haplotype (14-bpDEL/+3003T/+3010G/+3027C/+3035C/+3142C/+3187G/+3196C) was underrepresented in SLE (pcorr = 0.035).
  
  
  CONCLUSIONS
  These results indicate that HLA-G 3'UTR polymorphic sites, particularly +3142G and +3010C alleles, were associated with SLE susceptibility, whereas UTR-1 was associated with protection against development of SLE.
authors.affiliation: Department of Immunology, Aggeu Magalhães Research Center, Oswaldo Cruz Foundation, Recife-PE, Brazil. norma.lucena@hotmail.com
authors.forename: Norma
authors.initials: N
authors.lastname: Lucena-Silva
authors.forename: Veridiana Sales Barbosa
authors.initials: VS
authors.lastname: de Souza
authors.forename: Renan Garcia
authors.initials: RG
authors.lastname: Gomes
authors.forename: Alex
authors.initials: A
authors.lastname: Fantinatti
authors.forename: Yara Costa Netto
authors.initials: YC
authors.lastname: Muniz
authors.forename: Rafael Sales
authors.initials: RS
authors.lastname: de Albuquerque
authors.forename: Alessandra Luna Ramos
authors.initials: AL
authors.lastname: Monteiro
authors.forename: George Tadeu Nunes
authors.initials: GT
authors.lastname: Diniz
authors.forename: Maria Rosângela Cunha Duarte
authors.initials: MR
authors.lastname: Coelho
authors.forename: Celso Teixeira
authors.initials: CT
authors.lastname: Mendes-Junior
authors.forename: Erick da Cruz
authors.initials: Eda C
authors.lastname: Castelli
authors.forename: Eduardo Antônio
authors.initials: EA
authors.lastname: Donadi
mesh_terms: D020413:3' Untranslated Regions; D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D001938:Brazil; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D059951:HLA-G Antigens / Q000235:genetics*; D006239:Haplotypes; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic
chemical_list: D020413:3' Untranslated Regions; D059951:HLA-G Antigens
keywords: GENETIC STUDIES; HLA ANTIGENS; SUSCEPTIBILITY; SYSTEMIC LUPUS ERYTHEMATOSUS