title: Thioflavin-T excimer formation upon interaction with amyloid fibers.
abstract: The molecular mechanism of the Thioflavin-T (Th-T) binding to amyloids remains unknown. By combining experimental analysis of Th-T excitation and emission spectra with theoretical calculations we suggest that Th-T fluorescence changes upon interaction with amyloids may arise from the formation of an excimer with an oblique angle of ~120 degrees.
authors.affiliation: Institut de Nanociència i nanotecnologia, Departament Fisicoquímica, Universitat de Barcelona, Joan XXIII s/n, E-08028-Barcelona, Spain. rsabate@ub.edu
authors.forename: Raimon
authors.initials: R
authors.lastname: Sabate
authors.forename: Luis
authors.initials: L
authors.lastname: Rodriguez-Santiago
authors.forename: Mariona
authors.initials: M
authors.lastname: Sodupe
authors.forename: Sven J
authors.initials: SJ
authors.lastname: Saupe
authors.forename: Salvador
authors.initials: S
authors.lastname: Ventura
mesh_terms: D000682:Amyloid / Q000737:chemistry* / Q000378:metabolism; D016229:Amyloid beta-Peptides / Q000737:chemistry / Q000378:metabolism; D052160:Benzothiazoles; D019281:Dimerization; D006863:Hydrogen-Ion Concentration; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D011328:Prions / Q000737:chemistry / Q000378:metabolism; D012997:Solvents / Q000737:chemistry; D013844:Thiazoles / Q000737:chemistry*
chemical_list: D000682:Amyloid; D016229:Amyloid beta-Peptides; D052160:Benzothiazoles; D010446:Peptide Fragments; D011328:Prions; D012997:Solvents; D013844:Thiazoles; C075222:amyloid beta-protein (1-42); C009462:thioflavin T