title: Direct object resolution by image subtraction: a new molecular ruler for nanometric measurements on complexed fluorophores.
abstract: A technique for measuring distances between two or more fluorophores spaced in the 10-100 nm range is described. We identify a linear correlation between the intensity-amplitude in the difference-image of single molecules undergoing fluorescence fluctuations and their separation. The transform is used to map distances between coupled fluorophores.
authors.affiliation: Department of Chemistry, School of Life Sciences, University of Sussex, Falmer, BN1 9QJ, UK.
authors.forename: Rémi L
authors.initials: RL
authors.lastname: Boulineau
authors.forename: Mark A
authors.initials: MA
authors.lastname: Osborne
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D013499:Surface Properties
chemical_list: D005456:Fluorescent Dyes