title: Effects of hydroxyapatite nanostructure on channel surface of porcine acellular dermal matrix scaffold on cell viability and osteogenic differentiation of human periodontal ligament stem cells.
abstract: A new nanostructured hydroxyapatite-coated porcine acellular dermal matrix (HAp-PADM) was fabricated by a biomimetic mineralization method. Human periodontal ligament stem cells were seeded on HAp-PADM and the effects of this scaffold on cell shape, cytoskeleton organization, cell viability, and osteogenic differentiation were examined. Periodontal ligament stem cells cultured on HAp-PADM exhibited different cell shape when compared with those on pure PADM. Moreover, HAp-PADM promoted cell viability and alkaline phosphatase activity significantly. Based on quantitative real-time polymerase chain reaction, the expression of bone-related markers runt-related transcription factor 2 (Runx2), osteopontin (OPN), and osteocalcin (OCN) upregulated in the HAp-PADM scaffold. The enhancement of osteogenic differentiation of periodontal ligament stem cells on the HAp-PADM scaffold was proposed based on the research results. The results of this study highlight the micro-nano, two-level, three-dimensional HAp-PADM composite as a promising scaffold for periodontal tissue engineering.
authors.affiliation: Shandong Provincial Key Laboratory of Oral Biomedicine, Department of Periodontology, Shandong University, Jinan, People’s Republic of China.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Ge
authors.forename: Ning
authors.initials: N
authors.lastname: Zhao
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Pishan
authors.initials: P
authors.lastname: Yang
mesh_terms: D062088:Acellular Dermis; D000469:Alkaline Phosphatase / Q000032:analysis / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D017886:Durapatite / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D018613:Microscopy, Confocal; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure*; D010012:Osteogenesis / Q000187:drug effects*; D053495:Osteopontin / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D010513:Periodontal Ligament / Q000166:cytology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology*; D013552:Swine; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; C493709:RUNX2 protein, human; D053495:Osteopontin; D017886:Durapatite; D000469:Alkaline Phosphatase
keywords: differentiation; hydroxyapatite; nanostructure; proliferation; scaffold; tissue engineering