title: Genome-wide identification and evolutionary and expression analyses of MYB-related genes in land plants.
abstract: MYB proteins constitute one of the largest transcription factor families in plants. Recent evidence revealed that MYB-related genes play crucial roles in plants. However, compared with the R2R3-MYB type, little is known about the complex evolutionary history of MYB-related proteins in plants. Here, we present a genome-wide analysis of MYB-related proteins from 16 species of ﬂowering plants, moss, Selaginella, and algae. We identified many MYB-related proteins in angiosperms, but few in algae. Phylogenetic analysis classified MYB-related proteins into five distinct subgroups, a result supported by highly conserved intron patterns, consensus motifs, and protein domain architecture. Phylogenetic and functional analyses revealed that the Circadian Clock Associated 1-like/R-R and Telomeric DNA-binding protein-like subgroups are >1 billion yrs old, whereas the I-box-binding factor-like and CAPRICE-like subgroups appear to be newly derived in angiosperms. We further demonstrated that the MYB-like domain has evolved under strong purifying selection, indicating the conservation of MYB-related proteins. Expression analysis revealed that the MYB-related gene family has a wide expression profile in maize and soybean development and plays important roles in development and stress responses. We hypothesize that MYB-related proteins initially diversified through three major expansions and domain shuffling, but remained relatively conserved throughout the subsequent plant evolution.
authors.affiliation: 1Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region of Ministry of Agriculture, Maize Research Institute of Sichuan Agricultural University, Chengdu, Sichuan 611130, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Du
authors.forename: Yong-Bin
authors.initials: YB
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Xie
authors.forename: Zhe
authors.initials: Z
authors.lastname: Liang
authors.forename: San-Jie
authors.initials: SJ
authors.lastname: Jiang
authors.forename: Shuang-Shuang
authors.initials: SS
authors.lastname: Zhang
authors.forename: Yu-Bi
authors.initials: YB
authors.lastname: Huang
authors.forename: Yi-Xiong
authors.initials: YX
authors.lastname: Tang
mesh_terms: D019143:Evolution, Molecular; D005091:Exons; D018507:Gene Expression Regulation, Developmental; D018745:Genome, Plant; D007438:Introns; D020626:Oncogene Proteins v-myb / Q000235:genetics*; D010802:Phylogeny; D010944:Plants / Q000235:genetics*
chemical_list: D020626:Oncogene Proteins v-myb
keywords: MYB-related transcription factors; classification; evolution; expression profile analysis; phylogenetic analysis