title: A novel indirect ELISA based on glycoprotein Gn for the detection of IgG antibodies against Rift Valley fever virus in small ruminants.
abstract: Rift Valley fever virus (RVFV) is an emerging zoonotic pathogen that causes high morbidity and mortality in humans and livestock. In this paper, we describe the cloning, expression and purification of RVFV glycoprotein Gn and its application as a diagnostic antigen in an indirect ELISA for the specific detection of RVF IgG antibodies in sheep and goats. The performance of this Gn based ELISA is validated using a panel of almost 2000 field samples from sheep and goats from Mozambique, Senegal, Uganda and Yemen. All serum samples were also tested by virus neutralization test (VNT), the gold standard method for RVFV serological testing. Compared to the VNT results the Gn based ELISA proved to have an excellent sensitivity (94.56%) and specificity (95.57%). Apart from establishing this new diagnostic assay, these results also demonstrate a close correlation between the presence of RVFV Gn and neutralizing antibodies.
authors.affiliation: Institute for Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut, 17493 Greifswald-Insel Riems, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Jäckel
authors.forename: M
authors.initials: M
authors.lastname: Eiden
authors.forename: A
authors.initials: A
authors.lastname: Balkema-Buschmann
authors.forename: M
authors.initials: M
authors.lastname: Ziller
authors.forename: P Jansen
authors.initials: PJ
authors.lastname: van Vuren
authors.forename: J T
authors.initials: JT
authors.lastname: Paweska
authors.forename: M H
authors.initials: MH
authors.lastname: Groschup
mesh_terms: D017954:Africa South of the Sahara / Q000453:epidemiology; D000818:Animals; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D006023:Glycoproteins / Q000378:metabolism*; D015511:Goat Diseases / Q000175:diagnosis* / Q000821:virology; D006041:Goats; D007074:Immunoglobulin G / Q000097:blood; D012295:Rift Valley Fever / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D012296:Rift Valley fever virus / Q000276:immunology*; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis* / Q000821:virology; D014764:Viral Proteins / Q000276:immunology / Q000378:metabolism; D015006:Yemen / Q000453:epidemiology
chemical_list: D006023:Glycoproteins; D007074:Immunoglobulin G; D014764:Viral Proteins
keywords: Glycoprotein Gn; Indirect IgG ELISA; Rift Valley fever virus; Small ruminants