title: [Study on in vitro/in vivo correlation of extracts from Ligusticum chuanxiong after transdermal administration].
abstract: OBJECTIVE
  To study the transdermal permeation and in vivo pharmacokinetics of effective constituent ferulic acid from Ligusticum chuanxiong, in order to establish the in vitro/in vivo correlation in transdermal permeation.
  
  
  METHODS
  Franz diffusion cell was adopted in the in vitro transdermal permeation, with CD-1 nude rat abdominal skin as the permeation medium. Linear probes were implanted in CD-1 nude rats. With PBS as perfusate, microdialysis was employed to study the pharmacokinetics. Ferulic acid concentrations in the receptor solution and dialysate were assessed by high performance liquid chromatography (HPLC). The study on correlation between in vitro and in vivo data was conducted by deconvolution methods.
  
  
  RESULTS
  The transdermal permeation rate of ferulic acid from Ligusticum chuanxiong was (0.094 4 +/- 0.049 4) microg x cm2 x min, with Cmax of ferulic acid being 808.91 microg x L(-1), and Tmax being 183 min after dermal administration. The in vitro/in vivo correlation was 93.61.
  
  
  CONCLUSIONS
  Ferulic acid in extracts from L. chuanxiong can quickly penetrate skins. By using the in vitro/in vivo correlation in transdermal permeation, simple in vitro transdermal permeation method can be adopted to study the changes in its pharmacokinetics.
authors.affiliation: School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China. baijie22811@163.com
authors.forename: Jie
authors.initials: J
authors.lastname: Bai
authors.forename: Yang
authors.initials: Y
authors.lastname: Lu
authors.forename: Shou-Ying
authors.initials: SY
authors.lastname: Du
authors.forename: Cong-Min
authors.initials: CM
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Peng-Yue
authors.initials: PY
authors.lastname: Li
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000008:administration & dosage / Q000493:pharmacokinetics*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000493:pharmacokinetics*; D066298:In Vitro Techniques; D030004:Ligusticum; D008657:Metabolic Clearance Rate; D010539:Permeability; D051381:Rats; D011923:Rats, Nude; D012867:Skin / Q000378:metabolism*; D012869:Skin Absorption
chemical_list: D003373:Coumaric Acids; D004365:Drugs, Chinese Herbal; C004999:ferulic acid; C101574:chuangxiong extract