title: [Preparation and identification of polyclonal antibody to serotype I Mareks disease virus sorf2 protein].
abstract: OBJECTIVE
  To obtain mice and rabbit polyclonal antibody of serotype I Marek's disease virus (MDV) sorf2 protein with higher titer and to identify the specificity.
  
  
  METHODS
  Using serotype I MDV GX0101 as template, we amplified sorf2 gene and then cloned it into pET-28a (+) and pET-32a (+) respectively. The recombinant plasmid pET-28a-sorf2 and pET-32a-sorf2 was separately transformed into Escherichia coli BL21 (Rosetta) competent cell which was induced with isopropylthio-beta-D-galactoside (IPTG). After purification, immuned 6-8 Balb/c mice and adult New Zealand white rabbit with the purified fusion protein and the anti-sorf2 polyclonal antibody were prepared. The specificity of the serum was detected by Western blot and the indirect immunofluorescence assay (IFA) method.
  
  
  RESULTS
  Serotype I MDV sorf2 protein was expressed successfully in the recombinant E coli. Mice and rabbit anti-sorf2 polyclonal antibody with higher titer could react with sorf2 protein specifically.
  
  
  CONCLUSIONS
  The prepared anti-sorf2 polyclonal antibody could identify MDV sorf2 gene deletion strain specifically. In addition, it could be used for differential of MDV vaccine poison HVT and serotype I MDV, which was useful for the separation and identification of clinical MDV.
authors.affiliation: Animal Science and Technology College, Shandong Agricultural University, Tai' an 271018, China. cnydia@163.com
authors.forename: Ning
authors.initials: N
authors.lastname: Cui
authors.forename: Shuai
authors.initials: S
authors.lastname: Su
authors.forename: Jiuqing
authors.initials: J
authors.lastname: Li
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
authors.forename: Yanpeng
authors.initials: Y
authors.lastname: Li
authors.forename: Jiabo
authors.initials: J
authors.lastname: Ding
authors.forename: Zhizhong
authors.initials: Z
authors.lastname: Cui
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D015153:Blotting, Western; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D008381:Herpesvirus 2, Gallid / Q000235:genetics / Q000276:immunology*; D008380:Marek Disease / Q000276:immunology* / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D030781:Organisms, Genetically Modified; D010957:Plasmids; D011817:Rabbits; D011994:Recombinant Proteins; D017384:Sequence Deletion; D013047:Specific Pathogen-Free Organisms
chemical_list: D000914:Antibodies, Viral; D001426:Bacterial Proteins; D011994:Recombinant Proteins