title: Surface enhanced resonance Raman scattering (SERRS)--a first example of its use in multiplex genotyping.
abstract: Accurate multiplex genotyping of the mutational status of the cystic fibrosis gene with the sensitive and selective SERRS technique is reported. This new assay does not require separation of the various alleles and shows that SERRS is a realistic alternative detection technique for use in modern DNA assays.
authors.affiliation: Department of Pure and Applied Chemistry, University of Strathclyde, Glasgow, UK. duncan.graham@strath.ac.uk
authors.forename: D
authors.initials: D
authors.lastname: Graham
authors.forename: B J
authors.initials: BJ
authors.lastname: Mallinder
authors.forename: D
authors.initials: D
authors.lastname: Whitcombe
authors.forename: W E
authors.initials: WE
authors.lastname: Smith
mesh_terms: D000483:Alleles; D003550:Cystic Fibrosis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D017931:DNA Primers / Q000737:chemistry / Q000378:metabolism; D005838:Genotype; D006720:Homozygote; D006801:Humans; D060885:Multiplex Polymerase Chain Reaction; D020411:Oligonucleotide Array Sequence Analysis; D012235:Rhodamines / Q000737:chemistry; D013059:Spectrum Analysis, Raman
chemical_list: D017931:DNA Primers; D012235:Rhodamines