title: Glucagon-like peptide-1 upregulates visfatin expression in 3T3-L1 adipocytes.
abstract: The incretin hormone glucagon-like peptide-1 (GLP-1) exerts important functions in controlling glucose homeostasis. Many studies have revealed molecular targets of GLP-1, but its influence on adipokines has not been determined. Visfatin, a recently discovered adipokine, has been shown to attenuate insulin resistance by binding to insulin receptor. Our study shows that GLP-1 induced secretion of visfatin into the culture medium of 3T3-L1 adipocytes due to increased visfatin mRNA expression. Furthermore, the effect of GLP-1 on visfatin was dose- and time-dependent. H89, a protein kinase A inhibitor, prevented the induction of visfatin expression by GLP-1. Moreover, inhibition of NF-κB by PDTC reduced the basal visfatin release while having no effect on the transcription regulation by GLP-1. In addition, GLP-1 alleviated the decrease of visfatin mRNA expression under endoplasmic reticulum stress induced by thapsigargin. Taken together, our study suggests that GLP-1 promotes the novel insulin-mimetic adipocytokine visfatin expression via the PKA pathway and might influence glucose metabolism.
authors.affiliation: Department of Endocrinology, Shanghai First People's Hospital, Shanghai Jiaotong University, Shanghai, China.
authors.forename: R
authors.initials: R
authors.lastname: Liu
authors.forename: X
authors.initials: X
authors.lastname: Ding
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: M
authors.initials: M
authors.lastname: Wang
authors.forename: Y
authors.initials: Y
authors.lastname: Peng
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects / Q000201:enzymology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D017868:Cyclic AMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects / Q000235:genetics; D052216:Glucagon-Like Peptide 1 / Q000494:pharmacology*; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D054409:Nicotinamide Phosphoribosyltransferase / Q000235:genetics* / Q000378:metabolism; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D015533:Transcriptional Activation / Q000187:drug effects / Q000235:genetics; D015854:Up-Regulation / Q000187:drug effects*
chemical_list: D016328:NF-kappa B; D047428:Protein Kinase Inhibitors; D012333:RNA, Messenger; D052216:Glucagon-Like Peptide 1; D054409:Nicotinamide Phosphoribosyltransferase; D017868:Cyclic AMP-Dependent Protein Kinases