title: The loop-mediated isothermal amplification assay for rapid diagnosis of Babesia canis canis infections in dogs.
abstract: The aim of this study was to use a rapid and easy DNA-based test, the loop-mediated isothermal amplification (LAMP), for diagnosis of Babesia canis canis infections in dogs. 10 DNA samples of 18S RNA-A and 10 DNA samples of 18S RNA-B of B. canis canis were used in the study. LAMP method could successfully detect DNA in all examined samples down to 0.1 pg dilution. Obtained results suggest that this method has high specificity and sensitivity and can be applied in analytical laboratories in diagnosis of canine babesiosis.
authors.affiliation: Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Głeboka 30, 20-612 Lublin, Poland. ukaszek0@wp.pl
authors.forename: Ł
authors.initials: Ł
authors.lastname: Adaszek
authors.forename: M
authors.initials: M
authors.lastname: Jankowska
authors.forename: M
authors.initials: M
authors.lastname: Kalinowski
authors.forename: T
authors.initials: T
authors.lastname: Banach
authors.forename: D
authors.initials: D
authors.lastname: Wułupek
authors.forename: S
authors.initials: S
authors.lastname: Winiarczyk
mesh_terms: D000818:Animals; D001403:Babesia / Q000235:genetics / Q000302:isolation & purification*; D001404:Babesiosis / Q000175:diagnosis / Q000662:veterinary*; D004247:DNA / Q000235:genetics; D004283:Dog Diseases / Q000175:diagnosis / Q000469:parasitology*; D004285:Dogs; D021141:Nucleic Acid Amplification Techniques / Q000662:veterinary*; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012680:Sensitivity and Specificity
chemical_list: D012337:RNA, Ribosomal, 18S; D004247:DNA