title: Metabolic glycan imaging by isonitrile-tetrazine click chemistry.
abstract: Seeing the sugar coating: N-Acetyl-glucosamine and mannosamine derivatives tagged with an isonitrile group are metabolically incorporated into cell-surface glycans and can be detected with a fluorescent tetrazine. This bioorthogonal isonitrile-tetrazine ligation is also orthogonal to the commonly used azide-cyclooctyne ligation, and so will allow simultaneous detection of the incorporation of two different sugars.
authors.affiliation: Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK.
authors.forename: Shaun
authors.initials: S
authors.lastname: Stairs
authors.forename: André A
authors.initials: AA
authors.lastname: Neves
authors.forename: Henning
authors.initials: H
authors.lastname: Stöckmann
authors.forename: Yelena A
authors.initials: YA
authors.lastname: Wainman
authors.forename: Heather
authors.initials: H
authors.lastname: Ireland-Zecchini
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Brindle
authors.forename: Finian J
authors.initials: FJ
authors.lastname: Leeper
mesh_terms: D000117:Acetylglucosamine / Q000737:chemistry; D000818:Animals; D001710:Biotin / Q000737:chemistry; D045744:Cell Line, Tumor; D057930:Click Chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D006595:Hexosamines / Q000737:chemistry; D051379:Mice; D018613:Microscopy, Confocal; D009570:Nitriles / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D013777:Tetrazoles / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D006595:Hexosamines; D009570:Nitriles; D011134:Polysaccharides; D013777:Tetrazoles; C008322:mannosamine; D001710:Biotin; D000117:Acetylglucosamine