title: [Determination of terpene lactones in Ginkgo biloba leaves in different ages by UPLC-TQ-MS].
abstract: OBJECTIVE
  To establish an ultra-high performance liquid chromatography coupled with triple quadrupole mass (UPLC-TQ-MS) for determination of four terpene lactones.
  
  
  METHODS
  Chromatographic separation was carried out on a ACQUITY UPLC BEH C18 column (2.1 mm x 100 mm, 1.7 microm) with isocratic elution of 70% methanol at a flow rate of 0.4 mL x min(-1), the column temperature was set at 30 degrees C; Waters Xevo TQ worked in multiple reaction monitoring mode.
  
  
  RESULTS
  All calibration curves were linear (r > 0.990 3) over the tested ranges. The average recoveries ranged from 98.83% to 103.9% with RSD value below 3.0%. The contents of total terpene lactones in Ginkgo biloba leaves were significantly different in different ages. The contents in the leaves of young ginkgo tree were higher than that in old tree.
  
  
  CONCLUSIONS
  The method was simple and fast with high precision, sensitivity and repeatability, which can be used for qualitative and quantitative analysis of terpene lactones in G. biloba leaves.
authors.affiliation: Jiangsu Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae, Nanjing University of Chinese Medicine, Nanjing 210046, China. yaobest@163.com
authors.forename: Xin
authors.initials: X
authors.lastname: Yao
authors.forename: Gui-Sheng
authors.initials: GS
authors.lastname: Zhou
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Tang
authors.forename: Ye-Fei
authors.initials: YF
authors.lastname: Qian
authors.forename: Er-Xin
authors.initials: EX
authors.lastname: Shang
authors.forename: Shu-Lan
authors.initials: SL
authors.lastname: Su
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Qian
authors.forename: Jin-Ao
authors.initials: JA
authors.lastname: Duan
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D020441:Ginkgo biloba / Q000737:chemistry*; D007783:Lactones / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D018515:Plant Leaves / Q000737:chemistry*; D015203:Reproducibility of Results; D013729:Terpenes / Q000032:analysis*; D013997:Time Factors
chemical_list: D007783:Lactones; D013729:Terpenes