title: A physiological role for the supramolecular organization of rhodopsin and transducin in rod photoreceptors.
abstract: Vertebrate vision in rod photoreceptors begins when a photon hits the visual pigment rhodopsin (Rh) and triggers the phototransduction cascade. Although the fine biochemical and biophysical details of this paradigmatic signalling pathway have been studied for decades, phototransduction still presents unclear mechanistic aspects. Increasing lines of evidence suggest that the visual pigment rhodopsin (Rh) is natively organized in dimers on the surface of disc membranes, and may form higher order "paracrystalline" assemblies, which are not easy to reconcile with the classical collision-coupling mechanistic scenario evoked to explain the extremely fast molecular processes required in phototransduction. The questioned and criticized existence of paracrystalline Rh rafts can be fully accepted only if it can be explained in functional terms by a solid mechanistic picture. Here we discuss how recent data suggest a physiological role for supramolecular assemblies of Rh and its cognate G protein transducin (Gt), which by forming transient complexes in the dark may ensure rapid activation of the cascade even in a crowded environment that, according to the classical picture, would otherwise stop the cascade.
authors.affiliation: Department of Life Sciences and Reproduction, Section of Biological Chemistry, and Centre for Biomedical Computing, University of Verona, Strada le Grazie 8, 37134 Verona, Italy. daniele.dellorco@univr.it
authors.forename: Daniele
authors.initials: D
authors.lastname: Dell'Orco
mesh_terms: D000818:Animals; D006801:Humans; D007700:Kinetics; D008027:Light; D055537:Light Signal Transduction; D021962:Membrane Microdomains / Q000378:metabolism; D008954:Models, Biological; D011485:Protein Binding; D021381:Protein Transport; D017948:Retinal Rod Photoreceptor Cells / Q000378:metabolism* / Q000528:radiation effects; D012243:Rhodopsin / Q000378:metabolism*; D015293:Transducin / Q000378:metabolism*
chemical_list: D012243:Rhodopsin; D015293:Transducin