title: Genetic diversity in Napier grass (Pennisetum purpureum) cultivars: implications for breeding and conservation.
abstract: Napier grass is an important forage crop for dairy production in the tropics; as such, its existing genetic diversity needs to be assessed for conservation. The current study assessed the genetic variation of Napier grass collections from selected regions in Eastern Africa and the International Livestock Research Institute Forage Germplasm-Ethiopia. The diversity of 281 cultivars was investigated using five selective amplified fragment length polymorphism (AFLP) markers and classical population genetic parameters analysed using various software. The number of bands generated was 216 with fragments per primer set ranging from 50 to 115. Mean percentage polymorphic loci was 63.40. Genetic diversity coefficients based on Nei's genetic diversity ranged from 0.0783 to 0.2142 and Shannon's information index ranged from 0.1293 to 0.3445. The Fst value obtained was moderately significant (Fst = 0.1688). Neighbour-joining analysis gave two distinct clusters which did not reflect geographical locations. Analysis of molecular variance showed all variance components to be highly significant (P < 0.001), indicating more variation within (91 %) than between populations (9 %). Results suggested moderate genetic differentiation among Napier grass populations sampled, which could imply a high germplasm exchange within the region. The AFLP markers used in this study efficiently discriminate among cultivars and could be useful in identification and germplasm conservation.
authors.affiliation: Biotechnology and Biodiversity , Kenya Agricultural Research Institute , PO Box 14733, Nairobi 00800 , Kenya ; Biochemistry and Molecular Biology Department , Egerton University , PO Box 563, Egerton 20115 , Kenya ; Biosciences Eastern and Central Africa (BecA) at ILRI Hub , PO Box 30709, Nairobi 00100 , Kenya.
authors.forename: Bramwel W
authors.initials: BW
authors.lastname: Wanjala
authors.forename: Meshack
authors.initials: M
authors.lastname: Obonyo
authors.forename: Francis N
authors.initials: FN
authors.lastname: Wachira
authors.forename: Alice
authors.initials: A
authors.lastname: Muchugi
authors.forename: Margaret
authors.initials: M
authors.lastname: Mulaa
authors.forename: Jagger
authors.initials: J
authors.lastname: Harvey
authors.forename: Robert A
authors.initials: RA
authors.lastname: Skilton
authors.forename: Janice
authors.initials: J
authors.lastname: Proud
authors.forename: Jean
authors.initials: J
authors.lastname: Hanson
keywords: AFLP; Napier grass; conservation; cultivars; genetic diversity; germplasm