title: In vitro effects of resveratrol on oxidative stress in diabetic platelets.
abstract: To evaluate the in vitro effects of resveratrol (RSV) incubation on platelets from compensated and decompensated diabetic patients in order to use it as an adjuvant therapy. The study was performed on 77 diabetic patients and divided into two phases: 29 compensated and 48 decompensated diabetic platelets were analyzed at recruitment (T₀) and after in vitro RSV incubation (20 μg/ml) for 3 h at 37 °C (T₁). Lipoperoxide and nitric oxide (NO) levels, superoxide dismutase (SOD) and Na(+)/K(+) ATPase activities, total antioxidant capacity (TAC), and membrane fluidity tested by anisotropy of fluorescent probes TMA-DPH and DPH were determined. In vitro RSV incubation counteracts oxidative damage associated with diabetes and its complications; it is able to improve platelet function through augmented membrane fluidity and Na(+)/K(+) ATPase activity; it enhances antioxidant systems' functionality by increasing NO levels, SOD activity, and TAC and by decreasing lipoperoxide levels in both compensated and decompensated patients. Such platelet functionality enhancement suggests a new method of secondary prevention of complications associated with platelet dysfunction. Being free from one of the major risks associated with many antidiabetic agents, it can be assumed that RSV utilization in the diabetic diet may have a preventive and protective role in the progression of diabetic oxidative damage.
authors.affiliation: Section of Biochemistry, Biology and Physics, Department of Clinical Sciences, Faculty of Medicine, Marche Polytechnic University, Via Tronto 10, 60128, Ancona, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Raffaelli
authors.forename: A
authors.initials: A
authors.lastname: Vignini
authors.forename: A
authors.initials: A
authors.lastname: Giulietti
authors.forename: A
authors.initials: A
authors.lastname: Alidori
authors.forename: F
authors.initials: F
authors.lastname: Borroni
authors.forename: G
authors.initials: G
authors.lastname: Sforza
authors.forename: E
authors.initials: E
authors.lastname: Faloia
authors.forename: L
authors.initials: L
authors.lastname: Mazzanti
authors.forename: L
authors.initials: L
authors.lastname: Nanetti
mesh_terms: D000328:Adult; D000368:Aged; D000975:Antioxidants / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D002478:Cells, Cultured; D003922:Diabetes Mellitus, Type 1 / Q000097:blood*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D005260:Female; D006801:Humans; D008297:Male; D008560:Membrane Fluidity / Q000187:drug effects; D008875:Middle Aged; D018384:Oxidative Stress / Q000187:drug effects*; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D013267:Stilbenes; D000077185:Resveratrol