title: Immunization with biodegradable nanoparticles efficiently induces cellular immunity and protects against influenza virus infection.
abstract: The ability of vaccines to induce T cell responses is crucial for preventing diseases caused by viruses or bacteria. Nanoparticles (NPs) are considered an efficient tool for inducing potent immune responses. In this study, we describe a novel vaccination approach with biodegradable calcium phosphate (CaP) NPs that serve as carrier of immunoactive TLR9 ligand (CpG) combined with a viral Ag from the influenza A virus hemagglutinin. Functionalized CaP NPs were efficiently taken up by dendritic cells in vivo and elicited a potent T cell-mediated immune response in immunized mice with high numbers of IFN-γ-producing CD4(+) and CD8(+) effector T cells. Most importantly, both i.p. and intranasal immunization with these NPs offered protection in a mouse model of influenza virus infection. This study demonstrates the great potential of CaP NPs as a novel vaccination tool that offers substantial flexibility for several infection models.
authors.affiliation: Infection Immunology, Institute of Medical Microbiology, University Hospital Essen, University of Duisburg-Essen, 45122 Essen, Germany.
authors.forename: Torben
authors.initials: T
authors.lastname: Knuschke
authors.forename: Viktoriya
authors.initials: V
authors.lastname: Sokolova
authors.forename: Olga
authors.initials: O
authors.lastname: Rotan
authors.forename: Munisch
authors.initials: M
authors.lastname: Wadwa
authors.forename: Matthias
authors.initials: M
authors.lastname: Tenbusch
authors.forename: Wiebke
authors.initials: W
authors.lastname: Hansen
authors.forename: Peter
authors.initials: P
authors.lastname: Staeheli
authors.forename: Matthias
authors.initials: M
authors.lastname: Epple
authors.forename: Jan
authors.initials: J
authors.lastname: Buer
authors.forename: Astrid M
authors.initials: AM
authors.lastname: Westendorf
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D002130:Calcium Phosphates / Q000494:pharmacology; D005434:Flow Cytometry; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000276:immunology; D007111:Immunity, Cellular / Q000276:immunology*; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000737:chemistry / Q000276:immunology*; D007274:Injections, Intraperitoneal; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000737:chemistry; D013601:T-Lymphocytes / Q000276:immunology; D014611:Vaccination / Q000379:methods*
chemical_list: D002130:Calcium Phosphates; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines; C020243:calcium phosphate