title: Influence of the insertion of a cationic peptide on the size and shape of nanoliposomes: a light scattering investigation.
abstract: Nanoliposomes are widely used for drug delivery in the human body. Cell-penetrating peptides are amphiphilic peptides inserting in the lipid bilayer of these lipid vesicles to induce the fusion with target cells. Separation by size exclusion chromatography coupled with the analysis by light scattering detectors provides both the hydrodynamic radius and the radius of gyration of all the liposomes in a sample. In this paper, the influence of the insertion of a cationic peptide, K2LA12, on the size and shape of anionic liposomes has been studied by this approach. The results obtained highlighted an increase in size and a slight deformation of the lipid vesicles depending on the concentration of peptides incorporated into the lipid bilayers.
authors.affiliation: Unité de Génie Enzymatique et Cellulaire, UMR 6022 CNRS, Université de Picardie Jules Verne, 33 rue Saint-Leu, 80039, Amiens cedex, France. olivier.wattraint@u-picardie.fr
authors.forename: Olivier
authors.initials: O
authors.lastname: Wattraint
authors.forename: Imene
authors.initials: I
authors.lastname: Saadallah
authors.forename: Viviane
authors.initials: V
authors.lastname: Silva-Pires
authors.forename: Pascal
authors.initials: P
authors.lastname: Sonnet
authors.forename: Catherine
authors.initials: C
authors.lastname: Sarazin
mesh_terms: D057846:Cell-Penetrating Peptides / Q000737:chemistry*; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D010715:Phosphatidylglycerols / Q000737:chemistry*
chemical_list: D057846:Cell-Penetrating Peptides; D008081:Liposomes; D010715:Phosphatidylglycerols; C002773:dimyristoylphosphatidylglycerol; D004134:Dimyristoylphosphatidylcholine
keywords: Liposome size and shape; Membrane-active peptides; Multi-angle light scattering; Phospholipid; Photon correlation spectroscopy; Size exclusion chromatography