title: Lipid affinity beads: from identifying new lipid binding proteins to assessing their binding properties.
abstract: Lipid affinity beads can be used to identify novel proteins with lipid binding capacity or to determine binding prerequisites of known lipid-binding proteins. Here we describe several applications for which this tool can be used and which considerations have to be taken into account. In addition to a precise protocol, several suggestions are made for experimental setups to facilitate identification of in vivo lipid binding targets.
authors.affiliation: Swammerdam Institute for Life Sciences, Section Plant Physiology, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Fionn
authors.initials: F
authors.lastname: McLoughlin
authors.forename: Christa
authors.initials: C
authors.lastname: Testerink
mesh_terms: D017360:Arabidopsis / Q000378:metabolism; D001671:Biochemistry / Q000379:methods*; D008055:Lipids / Q000737:chemistry*; D018551:Solanum lycopersicum / Q000201:enzymology; D008565:Membrane Proteins / Q000378:metabolism; D008863:Microspheres; D010712:Phosphatidic Acids / Q000378:metabolism; D010730:Phosphoenolpyruvate Carboxylase / Q000378:metabolism; D011485:Protein Binding; D011506:Proteins / Q000378:metabolism*
chemical_list: D008055:Lipids; D008565:Membrane Proteins; D010712:Phosphatidic Acids; D011506:Proteins; D010730:Phosphoenolpyruvate Carboxylase