title: Steroidal glycosides from Reineckia carnea herba and their antitussive activity.
abstract: Two new steroidal glycosides, 1α,3α-dihydroxy-5β-pregn-16-en-20-one 3-O-α-L-rhamnopyranoside (1) and 1β,3β,27-trihydroxycholest-16-en-22-one 1,3-di-O-α-L-rhamnoside (2), along with seven known steroidal glycosides (3-9), were isolated from Reineckia carnea herba. Their structures were determined by detailed analysis of their 1D- and 2D-NMR and MS spectra. Compound 9 was isolated for the first time from the Reineckia genus. Except for 8, compounds 2, 3, 4, 5, 6, 7, and 9 displayed clear in vitro antitussive activity.
authors.affiliation: Development and Utilization Key Laboratory of Northeast Plant Materials, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, China.
authors.forename: Na
authors.initials: N
authors.lastname: Han
authors.forename: Ling Li
authors.initials: LL
authors.lastname: Chen
authors.forename: Yao
authors.initials: Y
authors.lastname: Wang
authors.forename: Rui
authors.initials: R
authors.lastname: Xue
authors.forename: Li Bo
authors.initials: LB
authors.lastname: Zou
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Yin
mesh_terms: D000818:Animals; D000996:Antitussive Agents / Q000737:chemistry* / Q000494:pharmacology*; D002783:Cholestenones / Q000737:chemistry / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods; D006027:Glycosides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D006168:Guinea Pigs; D066298:In Vitro Techniques; D019668:Liliaceae / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015394:Molecular Structure; D009126:Muscle Relaxation / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects; D010946:Plants, Medicinal; D011284:Pregnenolone / Q000031:analogs & derivatives; D014132:Trachea / Q000187:drug effects
chemical_list: C585786:1alpha,3alpha-dihydroxy-5beta-pregn-16-en-20-one 3-O-alpha-L-rhamnopyranoside; C585788:1beta,3beta,27-trihydroxycholest-16-en-22-one 1,3-di-O-alpha-L-rhamnoside; D000996:Antitussive Agents; D002783:Cholestenones; D006027:Glycosides; D011284:Pregnenolone