title: Excretion profile of corticosteroids in bovine urine compared with tissue residues after therapeutic and growth-promoting administration of dexamethasone.
abstract: The illicit use of dexamethasone as growth-promoting agent in animal breeding is still practiced within the EU constituting a health risk for meat consumers. An experimental study was developed to assess dexamethasone urinary excretion and tissue distribution (liver, kidney, and muscle) in male calves after therapeutic and growth-promoting administration. Urine and tissue samples collected from treated and untreated bovines were also investigated for the presence of other natural and synthetic corticosteroids (prednisolone, prednisone, hydrocortisone, and cortisone), in order to study a possible correlation with dexamethasone administration and to clarify prednisolone origin. Analyses were performed by a multi-residue LC-MS/MS method developed and validated according to the Commission Decision 2002/657/EC. The results confirm the rapid rate of dexamethasone urinary excretion, irrespective of the dosage, the duration and the route of administration, and the disappearance of cortisone and hydrocortisone during the treatment. Dexamethasone was distributed to the tissues where the elimination rate proceeded relatively slower as suggested by the presence of residues one month after the withdrawal of the therapeutic treatment. An increase in the number of positive findings for prednisolone, in association with higher levels of cortisone and hydrocortisone, was observed in urine samples collected from slaughterhouse rather than those collected at the farm. Prednisone residues were found only in one urine sample that showed the highest levels of prednisolone, hydrocortisone, and cortisone. The occurrence of prednisolone residues in urine and even in tissue samples confirms the endogenous nature of this molecule.
authors.affiliation: Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Roma, Italy. carolina.ferranti@iss.it
authors.forename: Carolina
authors.initials: C
authors.lastname: Ferranti
authors.forename: Marco
authors.initials: M
authors.lastname: Famele
authors.forename: Luca
authors.initials: L
authors.lastname: Palleschi
authors.forename: Elena
authors.initials: E
authors.lastname: Bozzetta
authors.forename: Marzia
authors.initials: M
authors.lastname: Pezzolato
authors.forename: Rosa
authors.initials: R
authors.lastname: Draisci
mesh_terms: D000818:Animals; D002417:Cattle; D003907:Dexamethasone / Q000031:analogs & derivatives* / Q000493:pharmacokinetics / Q000627:therapeutic use / Q000652:urine; D005938:Glucocorticoids / Q000493:pharmacokinetics / Q000627:therapeutic use / Q000652:urine*; D006133:Growth Substances / Q000493:pharmacokinetics / Q000627:therapeutic use / Q000652:urine; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D018482:Muscle, Skeletal / Q000378:metabolism; D011239:Prednisolone / Q000652:urine; D014018:Tissue Distribution
chemical_list: D005938:Glucocorticoids; D006133:Growth Substances; C004180:dexamethasone 21-phosphate; D003907:Dexamethasone; D011239:Prednisolone
keywords: Cattle; Dexamethasone; LC–MS/MS; Prednisolone; Tissue distribution; Urinary excretion