title: The effect of preoperative melatonin on nuclear erythroid 2-related factor 2 activation in patients undergoing coronary artery bypass grafting surgery.
abstract: During and after coronary artery bypass grafting (CABG), oxidative stress occurs. Finding an effective way to improve antioxidant response is important in CABG surgery. It has been shown that patients with coronary heart disease have a low Melatonin production rate. The present study aimed to investigate the effects of Melatoninon nuclear erythroid 2-related factor 2(Nrf2) activity in patients undergoing CABG surgery. Thirty volunteers undergoing CABG were randomized to receive 10 mg oral Melatonin (Melatonin group, n = 15) or placebo (placebo group, n = 15) before sleeping for 1 month before surgery. The activated Nrf2 was measured twice by DNA-based ELISA method in the nuclear extract of peripheral blood mononuclear cells of patients before aortic clumps and 45 minutes after CABG operation. Melatonin administration was associated with a significant increase in both plasma levels of Melatonin and Nrf2 concentration in Melatonin group compared to placebo group, respectively (15.2 ± 4.6 pmol/L, 0.28 ± 0.01 versus 1.1 ± 0.59 pmol/L, 0.20 ± 0.07, P < 0.05). The findings of the present study provide preliminary data suggesting that Melatonin may play a significant role in the potentiation of the antioxidant defense and attenuate cellular damages resulting from CABG surgery via theNrf2 pathway.
authors.affiliation: Department of Physiology, Physiology Research Centre, Isfahan University of Medical Sciences, Hezar Jerib Avenue, Isfahan 73461-8174, Iran.
authors.forename: Shaghayegh
authors.initials: S
authors.lastname: Haghjooy Javanmard
authors.forename: Amin
authors.initials: A
authors.lastname: Ziaei
authors.forename: Saeid
authors.initials: S
authors.lastname: Ziaei
authors.forename: Ehsan
authors.initials: E
authors.lastname: Ziaei
authors.forename: Mohsen
authors.initials: M
authors.lastname: Mirmohammad-Sadeghi
mesh_terms: D000328:Adult; D000368:Aged; D001026:Coronary Artery Bypass; D005260:Female; D006801:Humans; D008297:Male; D008550:Melatonin / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D008875:Middle Aged; D051267:NF-E2-Related Factor 2 / Q000378:metabolism*; D011182:Postoperative Care; D011300:Preoperative Care
chemical_list: D051267:NF-E2-Related Factor 2; C495635:NFE2L2 protein, human; D008550:Melatonin