title: The polyserine domain of the lysyl-5 hydroxylase Jmjd6 mediates subnuclear localization.
abstract: Jmjd6 (jumonji-domain-containing protein 6) is an Fe(II)- and 2OG (2-oxoglutarate)-dependent oxygenase that catalyses hydroxylation of lysine residues in proteins involved in pre-mRNA splicing. Jmjd6 plays an essential role in vertebrate embryonic development and has been shown to modulate alternative splicing in response to hypoxic stress. In the present study we show that an alternatively spliced version of Jmjd6 lacking the polyS (polyserine) domain localizes to the nucleolus, predominantly in the fibrillar centre. Jmjd6 with the polyS domain deleted also interacts with nucleolar proteins. Furthermore, co-immunoprecipitation experiments and F2H (fluorescent 2-hybrid) assays demonstrate that Jmjd6 homo-oligomerization occurs in cells. In correlation with the observed variations in the subnuclear distribution of Jmjd6, the structure of Jmjd6 oligomers in vitro changes in the absence of the polyS domain, possibly reflecting the role of the polyS domain in nuclear/nucleolar shuttling of Jmjd6.
authors.affiliation: Institute of Molecular Toxicology and Pharmacology, Helmholtz Zentrum München-German Research Center for Environmental Health, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Wolf
authors.forename: Monica
authors.initials: M
authors.lastname: Mantri
authors.forename: Astrid
authors.initials: A
authors.lastname: Heim
authors.forename: Udo
authors.initials: U
authors.lastname: Müller
authors.forename: Erika
authors.initials: E
authors.lastname: Fichter
authors.forename: Mukram M
authors.initials: MM
authors.lastname: Mackeen
authors.forename: Lothar
authors.initials: L
authors.lastname: Schermelleh
authors.forename: Gregory
authors.initials: G
authors.lastname: Dadie
authors.forename: Heinrich
authors.initials: H
authors.lastname: Leonhardt
authors.forename: Catherine
authors.initials: C
authors.lastname: Vénien-Bryan
authors.forename: Benedikt M
authors.initials: BM
authors.lastname: Kessler
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Schofield
authors.forename: Angelika
authors.initials: A
authors.lastname: Böttger
mesh_terms: D002460:Cell Line; D002466:Cell Nucleolus / Q000378:metabolism / Q000648:ultrastructure; D002850:Chromatography, Gel; D015180:Electrophoresis, Gel, Two-Dimensional; D036681:Fluorescence Recovery After Photobleaching; D006367:HeLa Cells; D006801:Humans; D047468:Immunoprecipitation; D056484:Jumonji Domain-Containing Histone Demethylases / Q000737:chemistry / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D008240:Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase / Q000737:chemistry / Q000378:metabolism*; D012326:RNA Splicing / Q000235:genetics
chemical_list: C498707:JMJD6 protein, human; D056484:Jumonji Domain-Containing Histone Demethylases; D008240:Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase