title: Synthesis, characterization, and antibacterial activity of cross-linked chitosan-glutaraldehyde.
abstract: This present study deals with synthesis, characterization and antibacterial activity of cross-linked chitosan-glutaraldehyde. Results from this study indicated that cross-linked chitosan-glutaraldehyde markedly inhibited the growth of antibiotic-resistant Burkholderia cepacia complex regardless of bacterial species and incubation time while bacterial growth was unaffected by solid chitosan. Furthermore, high temperature treated cross-linked chitosan-glutaraldehyde showed strong antibacterial activity against the selected strain 0901 although the inhibitory effects varied with different temperatures. In addition, physical-chemical and structural characterization revealed that the cross-linking of chitosan with glutaraldehyde resulted in a rougher surface morphology, a characteristic Fourier transform infrared (FTIR) band at 1559 cm⁻¹, a specific X-ray diffraction peak centered at 2θ = 15°, a lower contents of carbon, hydrogen and nitrogen, and a higher stability of glucose units compared to chitosan based on scanning electron microscopic observation, FTIR spectra, X-ray diffraction pattern, as well as elemental and thermo gravimetric analysis. Overall, this study indicated that cross-linked chitosan-glutaraldehyde is promising to be developed as a new antibacterial drug.
authors.affiliation: State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China. libin0571@zju.edu.cn
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Chang-Lin
authors.initials: CL
authors.lastname: Shan
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhou
authors.forename: Yuan
authors.initials: Y
authors.lastname: Fang
authors.forename: Yang-Li
authors.initials: YL
authors.lastname: Wang
authors.forename: Fei
authors.initials: F
authors.lastname: Xu
authors.forename: Li-Rong
authors.initials: LR
authors.lastname: Han
authors.forename: Muhammad
authors.initials: M
authors.lastname: Ibrahim
authors.forename: Long-Biao
authors.initials: LB
authors.lastname: Guo
authors.forename: Guan-Lin
authors.initials: GL
authors.lastname: Xie
authors.forename: Guo-Chang
authors.initials: GC
authors.lastname: Sun
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D016956:Burkholderia cepacia / Q000187:drug effects*; D048271:Chitosan / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D003432:Cross-Linking Reagents / Q000737:chemistry; D024881:Drug Resistance, Bacterial; D005976:Glutaral / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D006358:Hot Temperature; D008855:Microscopy, Electron, Scanning; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013818:Thermogravimetry; D013997:Time Factors; D014961:X-Ray Diffraction
chemical_list: D000900:Anti-Bacterial Agents; D003432:Cross-Linking Reagents; D048271:Chitosan; D005976:Glutaral