title: Effects of estrogen and estrogenic compounds, 4-tert-octylphenol, and bisphenol A on the uterine contraction and contraction-associated proteins in rats.
abstract: We examined the effects of estradiol (E2), 4-tert-octylphenol (OP), and bisphenol A (BPA) on uterine contractions in immature rats. The expression and localization of contraction-associated proteins (CAPs), and contractility of rat uterus with a collagen gel contraction assay were analyzed. E2, OP, and BPA all increased oxytocin (OT)-related pathway, while the prostaglandin-related signaling was reduced. Interestingly, E2 and estrogenic compounds showed distinct effects on the contractile activity of uterine cells. E2 enhanced the contractility, while OP and BPA significantly decreased it. Immunohistochemical analysis of CAPs showed distinct regulation of prostaglandin F receptor localization by E2 and estrogenic compounds, which may explain the different contractile activities of those reagents. In summary, we demonstrate that E2, OP, and BPA regulate CAP expression in a similar manner in the immature rat uterus, however, the effects on contractile activity were modulated differently. These findings suggest that OP and BPA interfere with uterine contractility.
authors.affiliation: Laboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea. anbs@pusan.ac.kr
authors.forename: Beum-Soo
authors.initials: BS
authors.lastname: An
authors.forename: Hyo-Jin
authors.initials: HJ
authors.lastname: Ahn
authors.forename: Hong-Seok
authors.initials: HS
authors.lastname: Kang
authors.forename: Eui-Man
authors.initials: EM
authors.lastname: Jung
authors.forename: Hyun
authors.initials: H
authors.lastname: Yang
authors.forename: Eui-Ju
authors.initials: EJ
authors.lastname: Hong
authors.forename: Eui-Bae
authors.initials: EB
authors.lastname: Jeung
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000494:pharmacology*; D002478:Cells, Cultured; D052244:Endocrine Disruptors / Q000494:pharmacology*; D004958:Estradiol / Q000494:pharmacology* / Q000502:physiology; D004968:Estrogens, Non-Steroidal / Q000494:pharmacology; D005260:Female; D015870:Gene Expression / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D006911:Hydroxyprostaglandin Dehydrogenases / Q000235:genetics / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000187:drug effects / Q000502:physiology; D010636:Phenols / Q000494:pharmacology*; D021381:Protein Transport; D051381:Rats; D017207:Rats, Sprague-Dawley; D018045:Receptors, Oxytocin / Q000235:genetics / Q000378:metabolism; D011982:Receptors, Prostaglandin / Q000235:genetics / Q000378:metabolism; D014590:Uterine Contraction / Q000187:drug effects* / Q000378:metabolism; D014599:Uterus / Q000166:cytology / Q000187:drug effects / Q000502:physiology; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D004958:Estradiol; D004968:Estrogens, Non-Steroidal; D006911:Hydroxyprostaglandin Dehydrogenases; D010636:Phenols; D018045:Receptors, Oxytocin; D011982:Receptors, Prostaglandin; C035859:15-hydroxyprostaglandin dehydrogenase; C105260:4-tert-octylphenol; C006780:bisphenol A; D000098826:Bisphenol A Compounds