title: In vitro antifungal activity of antifungalmycin 702, a new polyene macrolide antibiotic, against the rice blast fungus Magnaporthe grisea.
abstract: Antifungalmycin 702, a novel polyene macrolide antibiotic produced by Streptomyces padanus JAU4234, strongly inhibited mycelial growth of the rice blast fungus, Magnaporthe grisea, with EC50 of 37 μg/ml and EC90 of 136 μg/ml. Significant reduction in the number of conidia was observed at above 20 μg/ml. Conidia germination and appressorium formation were also suppressed and were not viable with >40 μg/ml. When treated with antifungalmycin 702, hyphae morphology became irregular. Based on microscopic examination, antifungalmycin 702 may exert its antifungal activity by changing the structure of cell membranes and the cytoskeleton and interacting with the organelles. Antifungalmycin 702 thus has potential as a new fungicide in the treatment of rice blast disease.
authors.affiliation: Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai, 200032, China. zhqxiong@sibs.ac.cn
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Xiong
authors.forename: Xiao-Rong
authors.initials: XR
authors.lastname: Tu
authors.forename: Sai-Jin
authors.initials: SJ
authors.lastname: Wei
authors.forename: Lin
authors.initials: L
authors.lastname: Huang
authors.forename: Xun-Hang
authors.initials: XH
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Lu
authors.forename: Guo-Quan
authors.initials: GQ
authors.lastname: Tu
mesh_terms: D002462:Cell Membrane / Q000187:drug effects; D003599:Cytoskeleton / Q000187:drug effects; D005659:Fungicides, Industrial / Q000302:isolation & purification / Q000494:pharmacology*; D018942:Macrolides / Q000302:isolation & purification / Q000494:pharmacology*; D020082:Magnaporthe / Q000187:drug effects* / Q000254:growth & development; D050296:Microbial Viability / Q000187:drug effects; D025282:Mycelium / Q000187:drug effects / Q000254:growth & development; D015388:Organelles / Q000187:drug effects; D012275:Oryza / Q000382:microbiology; D011090:Polyenes / Q000302:isolation & purification / Q000494:pharmacology*; D013302:Streptomyces / Q000378:metabolism*
chemical_list: D005659:Fungicides, Industrial; D018942:Macrolides; D011090:Polyenes