title: Longitudinal peripheral blood lymphocyte subsets correlate with decreased disease activity in juvenile dermatomyositis.
abstract: OBJECTIVE
  To determine the clinical characteristics and subsets of peripheral blood lymphocytes (PBL), which correlate with decreased disease activity in patients with juvenile dermatomyositis (JDM).
  
  
  METHODS
  Peripheral blood mononuclear cells from 24 patients with JDM were collected at Mayo Clinic Rochester between 2007 and 2011. These were analyzed using fluorescence-activated cell sorting and flow cytometry. Clinical disease activity was determined by visual analog scales (VAS) collected in 2 consecutive visits and correlated with PBL subsets.
  
  
  RESULTS
  The change in CD3+CD69+ T cells correlated with the change in global VAS scores. The change in HLA-DR- CD11c+ myeloid dendritic cells also correlated with the change in extramuscular VAS scores. There were trends toward decreased levels of HLA-DR- CD11c+ cells with decreased muscle and global VAS scores, but these did not reach significance. The change in HLA-DR- CD123+ plasmacytoid dendritic cells negatively correlated with the change in muscle VAS scores. Although not statistically significant, decreased levels of CD3-CD16- CD56+ natural killer (NK) cells and HLA-DR- CD86+ myeloid dendritic cells, and increased levels of CD16+CD56- NK cells, correlated with decreased VAS scores.
  
  
  CONCLUSIONS
  Changes in CD3+CD69+ T cells, HLA-DR- CD11c+ myeloid dendritic cells, and HLA-DR- CD123+ plasmacytoid dendritic cells are associated with improved clinical course in JDM and could be used as markers for disease activity, but findings need to be verified in a larger, independent cohort. Lack of significant differences among most of our PBL subsets suggests that lymphocyte phenotyping may be difficult to definitively correlate with disease activity in JDM.
authors.affiliation: Division of Rheumatology, Departments of Medicine and Pediatrics and Health Sciences Research, Mayo Clinic Rochester, Rochester, Minnesota, USA. ernste.floranne@mayo.edu
authors.forename: Floranne C
authors.initials: FC
authors.lastname: Ernste
authors.forename: Cynthia S
authors.initials: CS
authors.lastname: Crowson
authors.forename: Consuelo Lopez
authors.initials: CL
authors.lastname: de Padilla
authors.forename: Molly S
authors.initials: MS
authors.lastname: Hein
authors.forename: Ann M
authors.initials: AM
authors.lastname: Reed
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D003713:Dendritic Cells / Q000276:immunology*; D003882:Dermatomyositis / Q000097:blood / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D007694:Killer Cells, Natural / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D016131:Lymphocyte Subsets / Q000276:immunology*; D008297:Male; D012720:Severity of Illness Index; D055815:Young Adult
keywords: DENDRITIC CELLS; JUVENILE DERMATOMYOSITIS; NATURAL KILLER CELLS; PERIPHERAL BLOOD LYMPHOCYTE CELL SUBTYPES; T CELLS