title: Hetero-oligonucleotide nanoscale tiles capable of two-dimensional lattice formation as testbeds for a rapid, affordable purification methodology.
abstract: New nanoscale hetero-oligonucleotide tiles are assembled from DNA, RNA and morpholino oligos and purified using size exclusion filtration. Homo-oligonucleotide tiles assembled from RP-cartridge processed DNA oligos are purified by nondenaturing gel electrophoresis. These tiles' purity and homogeneity are demonstrated by gel electrophoresis and their incorporation into two-dimensional arrays visualized by AFM. This purification methodology increases throughput and decreases costs for researchers who wish to screen multiple tiles for utilization in structural or analytical studies.
authors.affiliation: Chemistry Department, St. John's University, 8000 Utopia Parkway, Queens, NY 11439, USA. lukemanp@stjohns.edu
authors.forename: Philip S
authors.initials: PS
authors.lastname: Lukeman
mesh_terms: D004247:DNA / Q000737:chemistry; D016521:Electrophoresis, Gel, Pulsed-Field; D018625:Microscopy, Atomic Force; D060172:Morpholinos / Q000737:chemistry; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry* / Q000302:isolation & purification; D012313:RNA / Q000737:chemistry
chemical_list: D060172:Morpholinos; D009841:Oligonucleotides; D012313:RNA; D004247:DNA