title: Porcine reproductive and respiratory syndrome virus activates inflammasomes of porcine alveolar macrophages via its small envelope protein E.
abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) infection results in extensive tissue inflammation and damage, which are believed to be responsible for increased susceptibility to secondary infection and even for death. However, its pathogenic mechanisms are not fully understood. To explore the mechanism underlying the PRRSV-induced tissue inflammation and damage, we investigated whether PRRSV activates porcine alveolar macrophage (PAM) inflammasomes which mediate por-IL-1β maturation/release and subsequently induce tissue inflammation and injury. Our results showed that PRRSV and its small envelope protein E significantly increased IL-1β release from LPS-primed PAMs; however, only PRRSV not protein E significantly increased IL-1β release from no-LPS-primed PAMs, which indicates PRRSV can activate inflammasomes of PAMs by its encoded protein E. These results provide a molecular basis for the pathogenic mechanism of PRRSV on inducing extensive tissue inflammation and damage, and suggest that the inflammasome may provide a potential therapeutic target for PRRS prevention and treatment.
authors.affiliation: Laboratory of Molecular Virology and Immunology, College of Veterinary Medicine, Agricultural University of Hebei, Hebei Engineering and Technology Research Center of Veterinary Biological Products, Baoding, China.
authors.forename: Kao
authors.initials: K
authors.lastname: Zhang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Hou
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Zhong
authors.forename: Xiujin
authors.initials: X
authors.lastname: Li
authors.forename: Huihui
authors.initials: H
authors.lastname: Chen
authors.forename: Wenyan
authors.initials: W
authors.lastname: Li
authors.forename: Jiexia
authors.initials: J
authors.lastname: Wen
authors.forename: Liyue
authors.initials: L
authors.lastname: Wang
authors.forename: Weiquan
authors.initials: W
authors.lastname: Liu
authors.forename: Fei
authors.initials: F
authors.lastname: Zhong
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D054884:Host-Pathogen Interactions; D058847:Inflammasomes / Q000276:immunology*; D053583:Interleukin-1beta / Q000276:immunology; D016676:Macrophages, Alveolar / Q000276:immunology* / Q000821:virology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology*; D013552:Swine; D014759:Viral Envelope Proteins / Q000276:immunology*
chemical_list: D058847:Inflammasomes; D053583:Interleukin-1beta; D014759:Viral Envelope Proteins