title: (2S)-5, 2', 5'-trihydroxy-7-methoxyflavanone, a natural product from Abacopteris penangiana, presents neuroprotective effects in vitro and in vivo.
abstract: The aim of this study was to investigate the neuroprotective effects of (2S)-5, 2', 5'-trihydroxy-7-methoxyflavanone (TMF), a natural product from Abacopteris penangiana (Hook.) Ching, in oxidative stress-induced neurodegeneration models in vitro and in vivo. In PC12 cells, preincubation of TMF (3-20 μM) for 24 h decreased the dopamine-induced toxicity and attenuated the redox imbalance in PC12 cells through regulating the ratio of reduced glutathione/oxidized glutathione (GSH/GSSG), which is a sensitive marker of oxidative stress. Additionally, long-term intraperitoneal (i.p.) injection of TMF (4 or 8 mg/kg/day) for 2 weeks significantly improved the behavioral performance of D-galactose (D-gal) treated mice in a Morris water maze test. Biochemical analysis revealed that TMF inhibited the activation of AP-1 (activator protein-1) and upregulated the level of BDNF (brain derived neurophic factor) as well as the ratio of GSH/GSSG in the hippocampus of D-gal treated mice. Furthermore, western blotting analysis indicated that TMF increased phosphorylation of cAMP-response element-binding protein (CREB). Therefore, the natural product TMF possessed a potential for the treatment of neurodegenerative diseases.
authors.affiliation: Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation of Hubei Province, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, No. 13, Hangkong Road, Wuhan, 430030, China.
authors.forename: Han
authors.initials: H
authors.lastname: Wei
authors.forename: Guanghua
authors.initials: G
authors.lastname: Wu
authors.forename: Jinglou
authors.initials: J
authors.lastname: Chen
authors.forename: Xuenong
authors.initials: X
authors.lastname: Zhang
authors.forename: Chaomei
authors.initials: C
authors.lastname: Xiong
authors.forename: Yongfang
authors.initials: Y
authors.lastname: Lei
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.forename: Jinlan
authors.initials: J
authors.lastname: Ruan
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D004298:Dopamine / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D047309:Flavones / Q000302:isolation & purification / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D019803:Glutathione Disulfide / Q000378:metabolism; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism; D066298:In Vitro Techniques; D018782:Maze Learning / Q000187:drug effects; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D016716:PC12 Cells; D029625:Pteridaceae / Q000737:chemistry*; D051381:Rats
chemical_list: C560949:5,2',5'-trihydroxy-7-methoxyflavone; D047309:Flavones; D009419:Nerve Tissue Proteins; D018696:Neuroprotective Agents; D005978:Glutathione; D019803:Glutathione Disulfide; D004298:Dopamine