title: Isolation and characterization of a cryptic plasmid, pMBLR00, from Leuconostoc mesenteroides subsp. mesenteroides KCTC 3733.
abstract: A cryptic plasmid, pMBLR00, from Leuconostoc mesenteroides subsp. mesenteroides KCTC 3733 was isolated, characterized, and used for the construction of a cloning vector to engineer Leuconostoc species. pMBLR00 is a rolling circle replication plasmid, containing 3,370 base pairs. Sequence analysis revealed that pMBLR00 has 3 open reading frames: Cop (copy number control protein), Rep (replication protein), and Mob (mobilization protein). pMBLR00 replicates by rolling circle replication, which was confirmed by the presence of a conserved double-stranded origin and single-stranded DNA intermediates. An Escherichia coli-Leuconostoc shuttle vector, pMBLR02, was constructed and was able to replicate in Leuconostoc citreum 95. pMBLR02 could be a useful genetic tool for metabolic engineering and the genetic study of Leuconostoc species.
authors.affiliation: Department of Molecular Science and Technology, Ajou University, Suwon 443-749, Korea.
authors.forename: Han Seung
authors.initials: HS
authors.lastname: Chae
authors.forename: Jeong Min
authors.initials: JM
authors.lastname: Lee
authors.forename: Ju-Hoon
authors.initials: JH
authors.lastname: Lee
authors.forename: Pyung Cheon
authors.initials: PC
authors.lastname: Lee
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D004265:DNA Helicases / Q000235:genetics; D004261:DNA Replication; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D005822:Genetic Vectors; D005827:Genetics, Microbial / Q000379:methods; D007934:Leuconostoc / Q000235:genetics*; D008967:Molecular Biology / Q000379:methods; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010957:Plasmids / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA; D015534:Trans-Activators / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; C083180:Mob protein, Bacteria; D015534:Trans-Activators; C045264:replication initiator protein; D004265:DNA Helicases