title: Hesperidin prevents androgen deficiency-induced bone loss in male mice.
abstract: The purpose of this study was to examine whether hesperidin inhibits bone loss in androgen-deficient male mice. Male ddY mice aged 7 weeks underwent either a sham operation or orchidectomy (ORX) and were divided into five groups: a sham-operated group fed a control diet (Sham) based on AIN-93G formulation with corn oil instead of soy bean oil, an ORX group fed the control diet (ORX), a group fed the control diet containing 0.5% hesperidin (ORX + H), a group fed the control diet containing 0.7% α-glucosylhesperidin (ORX + αG), and a group fed the control diet containing 0.013% simvastatin (ORX + St). Four weeks after intervention, ORX mice showed a striking decrease in seminal vesicle weight, which was not affected by the administration of hesperidin, α-glucosylhesperidin, or simvastatin. Femoral BMD was significantly reduced by ORX, and bone loss was inhibited by the administration of hesperidin, α-glucosylhesperidin or simvastatin. Histomorphometric analysis showed that the bone volume and trabecular thickness were significantly lower, and the osteoclast number was higher in the distal femoral cancellous bone in the ORX group than in the Sham group, and these were normalized in the ORX + H, ORX + αG and ORX + St groups. These results indicate that hesperidin inhibited bone resorption and hyperlipidemia, in ORX mice, and the preventive effect was stronger than that observed in ovariectomized mice in our previous study.
authors.affiliation: Department of Nutrition and Life Science, Kanagawa Institute of Technology, 1030 Shimoogino, Atsugi, Kanagawa, Japan; Department of Clinical Dietetics and Human Nutrition, Josai University, 1-1, Keyakidai, Sakado, Saitama, Japan.
authors.forename: Hiroshige
authors.initials: H
authors.lastname: Chiba
authors.forename: Hyounju
authors.initials: H
authors.lastname: Kim
authors.forename: Akiyo
authors.initials: A
authors.lastname: Matsumoto
authors.forename: Satoko
authors.initials: S
authors.lastname: Akiyama
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Ishimi
authors.forename: Kazuharu
authors.initials: K
authors.lastname: Suzuki
authors.forename: Mariko
authors.initials: M
authors.lastname: Uehara
mesh_terms: D000728:Androgens / Q000172:deficiency* / Q000494:pharmacology; D000818:Animals; D015519:Bone Density / Q000187:drug effects; D001862:Bone Resorption / Q000188:drug therapy*; D002118:Calcium / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D005269:Femur / Q000187:drug effects / Q000473:pathology; D006569:Hesperidin / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D009919:Orchiectomy; D009929:Organ Size / Q000187:drug effects; D012669:Seminal Vesicles / Q000187:drug effects; D019821:Simvastatin / Q000494:pharmacology; D014280:Triglycerides / Q000097:blood
chemical_list: D000728:Androgens; D014280:Triglycerides; D002784:Cholesterol; D019821:Simvastatin; D006569:Hesperidin; D002118:Calcium
keywords: androgen deficiency; hesperidin; osteoporosis; statin