title: Expression of CD200 and CD200R regulatory molecules on the CD83+ monocyte-derived dendritic cells generated from patients with laryngeal cancer.
abstract: CD200 molecule may play a role in local tumor invasion and augmenting the metastatic capacity of squamous cell carcinoma. The objective of the study was to assess by means of flow cytometry the expression of CD200 and its receptor, CD200R, on CD83+ monocyte-derived dendritic cells (Mo-DCs), pulsed or not with autologous tumor cell lysates (aTCL) in patients who suffer from laryngeal carcinoma in comparison to healthy donors. The median value of CD200 mean fluorescence intensity (MFI) on the Mo-DCs pulsed with aTCL of the patients with laryngeal cancer was 61.94 and was statistically significantly higher than on the unpulsed Mo-DCs of these patients (24.81) and healthy donors (16.63), p = 0.0034 and p = 0.0004, respectively. Median MFI score of CD200R in specimen derived from patients with laryngeal cancer was 259.31 on Mo-DCs pulsed with aTCL, while in unpulsed Mo-DCs was 86.74 (p = 0.0035) and on the Mo-DCs from control group it was 67.51 (p = 0.0004). The obtained results showed a relation between the presence of laryngeal cancer and the expression of CD200 and CD200R molecules on the CD83+ Mo-DCs pulsed with autologous cancer cell lysates. This analysis may have implications for setting new therapeutic options for cancer immunotherapy in the future.
authors.affiliation: Department of Otolaryngology and Laryngeal Oncology, Medical University of Lublin, Lublin, Poland. janusz.klatka@wp.pl
authors.forename: Janusz
authors.initials: J
authors.lastname: Klatka
authors.forename: Ewelina
authors.initials: E
authors.lastname: Grywalska
authors.forename: Maria
authors.initials: M
authors.lastname: Klatka
authors.forename: Mansur
authors.initials: M
authors.lastname: Rahnama
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Polak
authors.forename: Jacek
authors.initials: J
authors.lastname: Rolinski
mesh_terms: D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000378:metabolism*; D000954:Antigens, Surface / Q000378:metabolism*; D003713:Dendritic Cells / Q000378:metabolism* / Q000473:pathology*; D005434:Flow Cytometry; D005453:Fluorescence; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism*; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008562:Membrane Glycoproteins / Q000378:metabolism*; D008875:Middle Aged; D009000:Monocytes / Q000473:pathology*; D064446:Orexin Receptors; D011956:Receptors, Cell Surface / Q000378:metabolism*; D000097802:CD83 Antigen
chemical_list: D015703:Antigens, CD; D000954:Antigens, Surface; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D064446:Orexin Receptors; D011956:Receptors, Cell Surface; D000097802:CD83 Antigen; C477337:CD200R1 protein, human; C042739:antigens, CD200