title: A transfection reporter for the prevention of false-negative results in molecular beacon experiments.
abstract: We previously developed a molecular beacon-type probe to detect the strand scission in cellular base excision repair and found that the phosphodiester linkages in the fluorophore/quencher linkers were cleaved. This reaction was applied to a transfection reporter, which contained the unmodified phosphodiester in the linker to another type of fluorophore. After cotransfection of cells with the probe and the reporter, the signals were used to detect the incision and to confirm the proper transfection, respectively. This method will contribute to the prevention of false-negative results in experiments using molecular beacon-type probes.
authors.affiliation: Division of Chemistry, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 560-8531, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Toga
authors.forename: Isao
authors.initials: I
authors.lastname: Kuraoka
authors.forename: Akira
authors.initials: A
authors.lastname: Yasui
authors.forename: Shigenori
authors.initials: S
authors.lastname: Iwai
mesh_terms: D000818:Animals; D004260:DNA Repair / Q000235:genetics*; D005188:False Negative Reactions; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry; D017930:Genes, Reporter / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D015335:Molecular Probes / Q000032:analysis / Q000737:chemistry*; D012680:Sensitivity and Specificity; D014162:Transfection / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D015335:Molecular Probes
keywords: Base excision repair; Molecular beacon; Phosphorothioate; Thymine glycol