title: Lgr5 expression as stem cell marker in human gastric gland and its relatedness with other putative cancer stem cell markers.
abstract: To explore Lgr5 as the possible stem cell marker in human gastric tissue, 259 normal gastric tissues and dissected gastric adenocarcinoma were analyzed by immunohistochemistry, immunofluorescence double staining and qRT-PCR. The results demonstrated that Lgr5 was expressed in the bottom of the normal gastric gland units, and showed a differential expression in gastric adenocarcinoma with varying differentiation. Lgr5 and Bmi1 were co-expressed within the same cells of gastric glands. CD26+, CD44+, ALDH1+ and CD133+ cells co-existed with Lgr5+ cells in the stem cell zone of adjacent normal gastric mucosa, and they were detectable in gastric adenocarcinoma but behaved differently. We concluded that Lgr5 may be the adult stem cell marker in human gastric epithelium; Lgr5 and Bmi1 may belong to the same stem cell population; Lgr5, CD26, CD44, ALDH1, and CD133 may be functionally-associated.
authors.affiliation: College of Life Sciences, Hebei University, Baoding, Hebei 071002, PR China.
authors.forename: Chen
authors.initials: C
authors.lastname: Wu
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Xie
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Yanan
authors.initials: Y
authors.lastname: Wang
authors.forename: Yawei
authors.initials: Y
authors.lastname: Guo
authors.forename: Huanna
authors.initials: H
authors.lastname: Tian
authors.forename: Yanshuang
authors.initials: Y
authors.lastname: Li
authors.forename: Wufang
authors.initials: W
authors.lastname: Fan
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D000080924:Aldehyde Dehydrogenase 1 Family; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics; D004848:Epithelium / Q000378:metabolism; D005753:Gastric Mucosa / Q000378:metabolism*; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D014411:Neoplastic Stem Cells / Q000166:cytology / Q000378:metabolism*; D063150:Polycomb Repressive Complex 1 / Q000235:genetics / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D050697:Retinal Dehydrogenase / Q000235:genetics / Q000378:metabolism; D012189:Retrospective Studies; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*
chemical_list: D015703:Antigens, CD; C069077:BMI1 protein, human; D014408:Biomarkers, Tumor; D007527:Isoenzymes; C116884:LGR5 protein, human; D043562:Receptors, G-Protein-Coupled; D000080924:Aldehyde Dehydrogenase 1 Family; C510223:ALDH1A1 protein, human; D050697:Retinal Dehydrogenase; D063150:Polycomb Repressive Complex 1