title: Characterization of the transporterB0AT3 (Slc6a17) in the rodent central nervous system.
abstract: BACKGROUND
  The vesicular B0AT3 transporter (SLC6A17), one of the members of the SLC6 family, is a transporter for neutral amino acids and is exclusively expressed in brain. Here we provide a comprehensive expression profile of B0AT3 in mouse brain using in situ hybridization and immunohistochemistry.
  
  
  RESULTS
  We confirmed previous expression data from rat brain and used a novel custom made antibody to obtain detailed co-labelling with several cell type specific markers. B0AT3 was highly expressed in both inhibitory and excitatory neurons. The B0AT3 expression was highly overlapping with those of vesicular glutamate transporter 2 (VGLUT2) and vesicular glutamate transporter 1 (VGLUT1). We also show here that Slc6a17mRNA is up-regulated in animals subjected to short term food deprivation as well as animals treated with the serotonin reuptake inhibitor fluoxetine and the dopamine/noradrenaline reuptake inhibitor bupropion.
  
  
  CONCLUSIONS
  This suggests that the B0AT3 transporter have a role in regulation of monoaminergic as well as glutamatergic synapses.
authors.affiliation: Department of Neuroscience, Functional Pharmacology, Uppsala University, Uppsala, Sweden.
authors.forename: Maria G A
authors.initials: MG
authors.lastname: Hägglund
authors.forename: Sofie V
authors.initials: SV
authors.lastname: Hellsten
authors.forename: Sonchita
authors.initials: S
authors.lastname: Bagchi
authors.forename: Anna
authors.initials: A
authors.lastname: Ljungdahl
authors.forename: Victor C O
authors.initials: VC
authors.lastname: Nilsson
authors.forename: Sonja
authors.initials: S
authors.lastname: Winnergren
authors.forename: Olga
authors.initials: O
authors.lastname: Stephansson
authors.forename: Juris
authors.initials: J
authors.lastname: Rumaks
authors.forename: Simons
authors.initials: S
authors.lastname: Svirskis
authors.forename: Vija
authors.initials: V
authors.lastname: Klusa
authors.forename: Helgi B
authors.initials: HB
authors.lastname: Schiöth
authors.forename: Robert
authors.initials: R
authors.lastname: Fredriksson
mesh_terms: D000818:Animals; D000928:Antidepressive Agents / Q000494:pharmacology; D002478:Cells, Cultured; D002490:Central Nervous System / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D004622:Embryo, Mammalian; D005260:Female; D005508:Food Deprivation; D005786:Gene Expression Regulation / Q000187:drug effects / Q000502:physiology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D050480:Plasma Membrane Neurotransmitter Transport Proteins / Q000235:genetics / Q000378:metabolism*; D011247:Pregnancy; D021381:Protein Transport / Q000502:physiology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017208:Rats, Wistar; D033921:Vesicular Transport Proteins / Q000737:chemistry / Q000378:metabolism
chemical_list: D000928:Antidepressive Agents; D009419:Nerve Tissue Proteins; D050480:Plasma Membrane Neurotransmitter Transport Proteins; C079036:Slc6a17 protein, rat; D033921:Vesicular Transport Proteins