title: Increased nitroxidative stress promotes mitochondrial dysfunction in alcoholic and nonalcoholic fatty liver disease.
abstract: Increased nitroxidative stress causes mitochondrial dysfunctions through oxidative modifications of mitochondrial DNA, lipids, and proteins. Persistent mitochondrial dysfunction sensitizes the target cells/organs to other pathological risk factors and thus ultimately contributes to the development of more severe disease states in alcoholic and nonalcoholic fatty liver disease. The incidences of nonalcoholic fatty liver disease continuously increase due to high prevalence of metabolic syndrome including hyperlipidemia, hypercholesterolemia, obesity, insulin resistance, and diabetes. Many mitochondrial proteins including the enzymes involved in fat oxidation and energy supply could be oxidatively modified (including S-nitrosylation/nitration) under increased nitroxidative stress and thus inactivated, leading to increased fat accumulation and ATP depletion. To demonstrate the underlying mechanism(s) of mitochondrial dysfunction, we employed a redox proteomics approach using biotin-N-maleimide (biotin-NM) as a sensitive biotin-switch probe to identify oxidized Cys residues of mitochondrial proteins in the experimental models of alcoholic and acute liver disease. The aims of this paper are to briefly describe the mechanisms, functional consequences, and detection methods of mitochondrial dysfunction. We also describe advantages and limitations of the Cys-targeted redox proteomics method with alternative approaches. Finally, we discuss various applications of this method in studying oxidatively modified mitochondrial proteins in extrahepatic tissues or different subcellular organelles and translational research.
authors.affiliation: Section of Molecular Pharmacology and Toxicology, Laboratory of Membrane Biochemistry and Biophysics, National Institute on Alcohol Abuse and Alcoholism, 9000 Rockville Pike, Bethesda, MD 20892, USA. bj.song@nih.gov
authors.forename: Byoung-Joon
authors.initials: BJ
authors.lastname: Song
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: Abdelmegeed
authors.forename: Lauren E
authors.initials: LE
authors.lastname: Henderson
authors.forename: Seong-Ho
authors.initials: SH
authors.lastname: Yoo
authors.forename: Jie
authors.initials: J
authors.lastname: Wan
authors.forename: Vishnudutt
authors.initials: V
authors.lastname: Purohit
authors.forename: James P
authors.initials: JP
authors.lastname: Hardwick
authors.forename: Kwan-Hoon
authors.initials: KH
authors.lastname: Moon
mesh_terms: D000818:Animals; D005234:Fatty Liver / Q000378:metabolism / Q000473:pathology*; D005235:Fatty Liver, Alcoholic / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D008928:Mitochondria / Q000378:metabolism / Q000473:pathology*; D065626:Non-alcoholic Fatty Liver Disease; D018384:Oxidative Stress; D040901:Proteomics; D026361:Reactive Nitrogen Species / Q000378:metabolism*
chemical_list: D026361:Reactive Nitrogen Species