title: [Expression of long noncoding RNA MALAT1 gene in human nasopharyngeal carcinoma cell lines and its biological significance].
abstract: OBJECTIVE
  To explore the expression of MALAT 1 in nasopharyngeal carcinoma (NPC) and its biological function.
  
  
  METHODS
  Real-time PCR was used to detect the expression of MALAT1 in 5-8F, C666-1, CNE-1, CNE-2, HONE-1, 6-10B and NP69 cell lines. CNE-1 cells engineered with MALAT1 RNA interference (RNAi) and RNA activation (RNAa) techniques were examined for changes in cell proliferation, invasion and metastasis using CCK8 assay, colony formation assay, Transwell in vitro invasion assay and wound-healing assay ability.
  
  
  RESULTS
  MALAT1 was highly expressed in 5-8F cells with a high metastatic potential, and lowly expressed in normal nasopharyngeal epithelium cells. Overexpression of MALAT1 by RNAa suppressed the expression of E-cadherin, promoted the expression of vimentin and enhanced the proliferation, invasion, and metastasis of CNE-1 cells.
  
  
  CONCLUSIONS
  MALAT1 can enhance the proliferation, invasion, and metastasis of CNE-1 cells.
authors.affiliation: Department of Pathology, Southern Medical University, Guangzhou, China. xielingying321@163.com
authors.forename: Linying
authors.initials: L
authors.lastname: Xie
authors.forename: Zhiyan
authors.initials: Z
authors.lastname: Hu
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Wang
authors.forename: Zuguo
authors.initials: Z
authors.lastname: Li
mesh_terms: D015703:Antigens, CD; D015820:Cadherins / Q000378:metabolism; D002277:Carcinoma; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D052138:Genes, Neoplasm; D006801:Humans; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology*; D034622:RNA Interference; D062085:RNA, Long Noncoding / Q000235:genetics*; D014746:Vimentin / Q000378:metabolism
chemical_list: D015703:Antigens, CD; C513384:CDH1 protein, human; D015820:Cadherins; C557041:MALAT1 long non-coding RNA, human; D062085:RNA, Long Noncoding; D014746:Vimentin