title: Kinetics and mechanism of reactions of the drug tiopronin with platinum(IV) complexes.
abstract: Tiopronin, a synthetic thiol-containing drug being used in treatments of cystinuria and certain types of rare arthritis, is also a hepatoprotective and a detoxifying agent. Many analytical methods have been developed based on its redox chemistry with metal ions/complexes, but the kinetic and mechanistic aspects are poorly understood. In this work, the oxidation of tiopronin by cisplatin prodrug and a model compound, cis-[Pt(NH3)2Cl4] and trans-[PtCl2(CN)4](2-), was investigated. The oxidation kinetics was followed by a stopped-flow spectrophotometer over a wide pH range under the pseudo first-order conditions of [Tiopronin]≫[Pt(IV)]. Time-resolved spectra were also recorded for both Pt(IV) complexes, enabling to establish an overall second-order rate law: -d[Pt(IV)]/dt=k'[Tiopronin][Pt(IV)], where k' pertains to observed second-order rate constants. Under the kinetic conditions, tiopronin was oxidized to form the tiopronin-disulfide exclusively as identified by mass spectrometry. A reaction mechanism was proposed, involving parallel reductions of the Pt(IV) complexes by the three protolytic tiopronin species as rate-determining steps. The rate constants for the rate-determining steps were derived. The fully deprotonated tiopronin is about 4×10(4) more reactive than its corresponding thiol form for both Pt(IV) complexes; the huge reactivity difference orchestrates closely with the fact that the nucleophilicity of thiolate is much higher than the corresponding thiol. Hence, the attack of the sulfur atom in thiol/thiolate of tiopronin on the axially-coordinated chloride in the Pt(IV) complexes is nucleophilic in nature in the rate-determining steps, resulting in a bridge formation and a subsequent bridged electron-transfer.
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China.
authors.forename: Shuying
authors.initials: S
authors.lastname: Huo
authors.forename: Hongmei
authors.initials: H
authors.lastname: Shi
authors.forename: Dongzhi
authors.initials: D
authors.lastname: Liu
authors.forename: Shigang
authors.initials: S
authors.lastname: Shen
authors.forename: Jiong
authors.initials: J
authors.lastname: Zhang
authors.forename: Changying
authors.initials: C
authors.lastname: Song
authors.forename: Tiesheng
authors.initials: T
authors.lastname: Shi
mesh_terms: D002945:Cisplatin / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D013058:Mass Spectrometry; D009944:Organoplatinum Compounds / Q000737:chemistry*; D010084:Oxidation-Reduction; D011355:Prodrugs / Q000737:chemistry; D008625:Tiopronin / Q000737:chemistry*
chemical_list: D009944:Organoplatinum Compounds; D011355:Prodrugs; C501018:diammineplatinum(IV) tetrachloride; D008625:Tiopronin; D002945:Cisplatin