title: Measurement of transporter associated with antigen processing 1 and tumor necrosis factor alpha expression in hepatitis B virus-related hepatocellular carcinoma and peritumor cirrhosis tissues using tissue chip technology.
abstract: OBJECTIVE
  The objective of this study was to explore the associations of expression of transporter associated with antigen processing 1 (TAP1) and tumor necrosis factor alpha (TNF-α with the occurrence and development of HBV-related hepatocellular carcinoma (HCC).
  
  
  METHODS
  The expression of TAP1 and TNF-α in 38 HCC, 32 peritumor liver cirrhosis and 28 normal liver tissues, were assessed by immunohistochemical assay using tissue microarray technology.
  
  
  RESULTS
  TAP1 and TNF-α were negative in normal liver tissue hut positive in HCC and peritumor cirrhosis tissue. There were no significant differences in the rates of positivity for TAP1 and TNF-α between HCC and peritumor cirrhosis tissue (p>0.05), but there was a significant difference when rates in HCC and peritumor cirrhosis tissue were compared with those in normal liver tissue (p<0.0001, p<0.01, respectively). The degree of differentiation of HCC was correlated with TNF-α expression (p<0.05), but not TAP1 expression (p>0.05), CONCLUSIONS: Major histocompatibility complex class I molecules are involved in HBV-related HCC. TNF-α plays an important role in liver cirrhosis and in formation and development of HCC following HBV infection. TNF-α can be used as an indicator of the degree of differentiation of HCC.
authors.affiliation: Department of Gastroenterology, Heilongjiang Province Hospital, Harbin, China. bingqiu07@163.com
authors.forename: Bing
authors.initials: B
authors.lastname: Qiu
authors.forename: Wei
authors.initials: W
authors.lastname: Jiang
authors.forename: Jiye
authors.initials: J
authors.lastname: Zhang
authors.forename: Yanying
authors.initials: Y
authors.lastname: Wang
authors.forename: Fengxiang
authors.initials: F
authors.lastname: Wei
authors.forename: Huijing
authors.initials: H
authors.lastname: Hu
authors.forename: Peiyi
authors.initials: P
authors.lastname: Zhang
authors.forename: Chunlin
authors.initials: C
authors.lastname: Shi
authors.forename: Jianying
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D018528:ATP-Binding Cassette Transporters / Q000032:analysis* / Q000502:physiology; D000328:Adult; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000737:chemistry* / Q000473:pathology; D005260:Female; D006509:Hepatitis B / Q000150:complications*; D006801:Humans; D008113:Liver Neoplasms / Q000737:chemistry* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D046888:Tissue Array Analysis / Q000379:methods*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis* / Q000502:physiology
chemical_list: D000071181:ATP Binding Cassette Transporter, Subfamily B, Member 2; D018528:ATP-Binding Cassette Transporters; C073119:TAP1 protein, human; D014409:Tumor Necrosis Factor-alpha