title: Parvovirus evades interferon-dependent viral control in primary mouse embryonic fibroblasts.
abstract: Engagement of innate viral sensors elicits a robust antiviral program via the induction of type I interferons (IFNs). Innate defense mechanisms against ssDNA viruses are not well defined. Here, we examine type I IFN induction and effectiveness in controlling a ssDNA virus. Using mouse embryonic fibroblasts (MEFs), we found that a murine parvovirus, minute virus of mice (MVMp), induced a delayed but significant IFN response. MEFs deficient in mitochondrial antiviral signaling protein (MAVS) mounted a wild-type IFN response to MVMp infection, indicating that RIG-I-dependent RNA intermediate recognition is not required for innate sensing of this virus. However, MVMp-induced IFNs, as well recombinant type I IFNs, were unable to inhibit viral replication. Finally, MVMp infected cells became unresponsive to Poly (I:C) stimulation. Together, these data suggest that the MVMp efficiently evades antiviral immune mechanisms imposed by type I IFNs, which may in part explain their efficient transmission between mice.
authors.affiliation: Department of Immunobiology, Yale University, New Haven, CT 06520, USA.
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Mattei
authors.forename: Susan F
authors.initials: SF
authors.lastname: Cotmore
authors.forename: Peter
authors.initials: P
authors.lastname: Tattersall
authors.forename: Akiko
authors.initials: A
authors.lastname: Iwasaki
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D000998:Antiviral Agents / Q000276:immunology* / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000276:immunology / Q000821:virology*; D007113:Immunity, Innate; D007370:Interferon Type I / Q000276:immunology* / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008915:Minute Virus of Mice / Q000187:drug effects / Q000276:immunology* / Q000472:pathogenicity*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D010322:Parvoviridae Infections / Q000276:immunology* / Q000821:virology; D012320:RNA Polymerase III; D011956:Receptors, Cell Surface; D014779:Virus Replication / Q000276:immunology; D000097905:Roundabout Proteins
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D000998:Antiviral Agents; D007370:Interferon Type I; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; D012320:RNA Polymerase III; D011956:Receptors, Cell Surface; C512612:MAVS protein, mouse; C118348:Robo3 protein, mouse; D000097905:Roundabout Proteins