title: EBER in situ hybridization for Epstein-Barr virus.
abstract: Epstein-Barr encoding region (EBER) in situ hybridization is the methodology of choice for the detection of the Epstein-Barr virus (EBV) in tissue sections. Because of the large numbers of copies of EBERs present in latently infected cells, non-isotopic methods can be used. Positive studies show staining in the nuclei of the EBV-infected cells, accentuating the chromatin and often excluding the nucleolus. False-negative results are most often the result of RNA degradation in the tissues, a finding that may be detected through the use of a polyT probe as a control for RNA preservation.
authors.affiliation: Clarient Pathology Services, Inc., Aliso Viejo, CA, USA.
authors.forename: Lawrence M
authors.initials: LM
authors.lastname: Weiss
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Chen
mesh_terms: D002460:Cell Line; D002467:Cell Nucleus / Q000235:genetics; D020031:Epstein-Barr Virus Infections / Q000175:diagnosis* / Q000821:virology; D004854:Herpesvirus 4, Human / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D017403:In Situ Hybridization; D016133:Polymerase Chain Reaction / Q000379:methods; D012367:RNA, Viral / Q000302:isolation & purification*; D014763:Viral Matrix Proteins / Q000302:isolation & purification
chemical_list: C099537:Epstein-Barr virus encoded RNA 1; D012367:RNA, Viral; D014763:Viral Matrix Proteins