title: miR-449b inhibits the proliferation of SW1116 colon cancer stem cells through downregulation of CCND1 and E2F3 expression.
abstract: Colorectal cancer is one of the leading causes of cancer-related mortality worldwide. Cancer stem cells are cell populations with stem cell nature presenting in tumor tissues and are the root of tumor formation and metastasis. CCND1 and E2F3 play important roles in cell cycle regulation. The 3'UTRs of CCND1 and E2F3 contain miR-449 binding sites. By transfecting pre-miR-449b and inhibiting miR-449b, we found that cell cycle, cell proliferation ability and cell cycle regulatory protein expression levels of colon cancer stem cells were altered. The correlation between CCND1, E2F3 and miR-449b showed that miR-449b could downregulate CCND1 and E2F3 expression. This, in turn, reduced the proliferative ability of colon cancer stem cells. These data suggest that miR-449b plays a tumor-suppressive role in colon cancer stem cells.
authors.affiliation: Department of General Surgery, Huashan Hospital Affiliated to Fudan University, Shanghai 200040, P.R. China.
authors.forename: Yantian
authors.initials: Y
authors.lastname: Fang
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Gu
authors.forename: Zhengyang
authors.initials: Z
authors.lastname: Li
authors.forename: Jianbin
authors.initials: J
authors.lastname: Xiang
authors.forename: Zongyou
authors.initials: Z
authors.lastname: Chen
mesh_terms: D002453:Cell Cycle / Q000235:genetics; D018797:Cell Cycle Proteins / Q000096:biosynthesis / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism*; D019938:Cyclin D1 / Q000096:biosynthesis*; D015536:Down-Regulation; D050699:E2F3 Transcription Factor / Q000096:biosynthesis*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: C530104:CCND1 protein, human; D018797:Cell Cycle Proteins; D050699:E2F3 Transcription Factor; C098319:E2F3 protein, human; C544530:MIRN449 microRNA, human; D035683:MicroRNAs; D019938:Cyclin D1