title: Micro-structurally detailed model of a therapeutic hydrogel injectate in a rat biventricular cardiac geometry for computational simulations.
abstract: Biomaterial injection-based therapies have showed cautious success in restoration of cardiac function and prevention of adverse remodelling into heart failure after myocardial infarction (MI). However, the underlying mechanisms are not well understood. Computational studies utilised simplified representations of the therapeutic myocardial injectates. Wistar rats underwent experimental infarction followed by immediate injection of polyethylene glycol hydrogel in the infarct region. Hearts were explanted, cryo-sectioned and the region with the injectate histologically analysed. Histological micrographs were used to reconstruct the dispersed hydrogel injectate. Cardiac magnetic resonance imaging data from a healthy rat were used to obtain an end-diastolic biventricular geometry which was subsequently adjusted and combined with the injectate model. The computational geometry of the injectate exhibited microscopic structural details found the in situ. The combination of injectate and cardiac geometry provides realistic geometries for multiscale computational studies of intra-myocardial injectate therapies for the rat model that has been widely used for MI research.
authors.affiliation: a Cardiovascular Research Unit, Chris Barnard Division of Cardiothoracic Surgery, Faculty of Health Sciences, University of Cape Town , Private Bag X3, 7935 Observatory , South Africa.
authors.forename: Mazin S
authors.initials: MS
authors.lastname: Sirry
authors.forename: Neil H
authors.initials: NH
authors.lastname: Davies
authors.forename: Karen
authors.initials: K
authors.lastname: Kadner
authors.forename: Laura
authors.initials: L
authors.lastname: Dubuis
authors.forename: Muhammad G
authors.initials: MG
authors.lastname: Saleh
authors.forename: Ernesta M
authors.initials: EM
authors.lastname: Meintjes
authors.forename: Bruce S
authors.initials: BS
authors.lastname: Spottiswoode
authors.forename: Peter
authors.initials: P
authors.lastname: Zilla
authors.forename: Thomas
authors.initials: T
authors.lastname: Franz
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D003198:Computer Simulation; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000627:therapeutic use*; D007267:Injections; D008297:Male; D009203:Myocardial Infarction / Q000503:physiopathology*; D051381:Rats; D017208:Rats, Wistar; D020257:Ventricular Remodeling
chemical_list: D001672:Biocompatible Materials; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate
keywords: computational modelling; hydrogel; image-based reconstruction; myocardial infarction; therapeutic injectate