title: Syntheses, characterization, antimicrobial and cytotoxic activities of pyridine-2,5-dicarboxylate complexes with 1,10-phenanthroline.
abstract: In this study, four mononuclear M(II)-pyridine-2,5-dicarboxylate (M = Co(II), Ni(II), Cu(II) and Zn(II) complexes with pyridine-2,5-dicarboxylic acid or isocinchomeronic acid, 1,10-phenanthroline (phen), [Co(Hpydc)(2)(phen)]·H(2)O (1), [Ni(pydc)(phen)(2)]·6.5H(2)O (2) [Cu(pydc)(phen)(H(2)O)(2)] (3) and [Zn(pydc)(phen)(H(2)O)(2)]·H(2)O (4) have been synthesized. Elemental, thermal and mass analyses, molar conductance, magnetic susceptibilities, IR and UV/vis spectroscopic studies have been performed to characterize the complexes. Subsequently, these ligands and complexes were tested for antimicrobial activity by disc diffusion method on Gram positive, negative bacteria and yeast. In addition, cytotoxic activity tests were performed on rat glioma (C6) cells by MTT viability assay for 24 and 48 h. Antimicrobial activity results demonstrated that when compared to the standard antibiotics, phen displayed the most effective antimicrobial effect. The effect of synthesized complexes was close to phen or less. Cytotoxic activity results showed that IC(50) value of phen was determined as 31 μM for 48 h. (1) and (2) compared to the alone ligand had less toxic activity. IC(50) values of (3) for 24 and 48 h treatments were 2.5 and 0.6 μM, respectively. IC(50) value of (4) for 48 h was 15 μM. In conclusion, phen, (3) and (4) may be useful as antibacterial and antiproliferative agents in the future.
authors.affiliation: Department of Chemistry, Faculty of Arts and Sciences, Dumlupinar University, 43820 Kutahya, Turkey. Electronic address: acolak@dumlupinar.edu.tr.
authors.forename: Alper Tolga
authors.initials: AT
authors.lastname: Colak
authors.forename: Pinar
authors.initials: P
authors.lastname: Oztopcu-Vatan
authors.forename: Ferdag
authors.initials: F
authors.lastname: Colak
authors.forename: Demet
authors.initials: D
authors.lastname: Akduman
authors.forename: Selda
authors.initials: S
authors.lastname: Kabadere
authors.forename: Ruhi
authors.initials: R
authors.lastname: Uyar
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D000935:Antifungal Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D016543:Central Nervous System Neoplasms / Q000188:drug therapy* / Q000473:pathology; D003035:Cobalt / Q000737:chemistry; D003300:Copper / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D005910:Glioma / Q000188:drug therapy* / Q000473:pathology; D015394:Molecular Structure; D009532:Nickel / Q000737:chemistry; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D010618:Phenanthrolines / Q000737:chemistry; D011725:Pyridines / Q000737:chemistry; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000187:drug effects; D013329:Structure-Activity Relationship; D015032:Zinc / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D000970:Antineoplastic Agents; D009942:Organometallic Compounds; D010618:Phenanthrolines; D011725:Pyridines; D003035:Cobalt; C043093:2,5-Pyridinedicarboxylic acid; D003300:Copper; D009532:Nickel; D015032:Zinc; C025205:1,10-phenanthroline
keywords: Antimicrobial activity; C6; Cytotoxicity; Metal complexes; Pyridine-2,5-dicarboxylate complexes