title: [Expression of high mobility group box 1 in gingival tissues of chronic periodontitis].
abstract: OBJECTIVE
  To investigate the expression of high mobility group box 1 (HMGB1) in gingival tissues of chronic periodontitis.
  
  
  METHODS
  Human peripheral blood mononuclear cells(PBMC) were stimulated with 1 microg x mL(-1) lipopolysaccharide (LPS) for 24 h or 48 h. Expression and release of HMGB1 were checked by immunofluorescence and enzyme-linked immunosorbent assay (ELISA), respectively. PBMC were stimulated with 100 ng x mL(-1) HMGB1 or 50 ng x mL(-1) tumor necrosis factor-alpha (TNF-alpha), the expressions of TNF-alpha and HMGB1 in the supernatant were studied by ELISA. Gingival tissues and gingival crevicular fluids (GCF) were collected from patients and healthy people. Expression of HMGB1 in gingival tissues and GCF was studied using immunofluorescence and ELISA, respectively.
  
  
  RESULTS
  HMGB1 was translocated from nucleus to cytosol in PBMC after LPS stimulation for 24 h. The content of HMGB1 in the supernatant from stimulated cells was significantly higher than that from unstimulated cells after 48 h (P < 0.01). HMGB1 was released by PBMC in response to TNF-alpha stimulation, it also stimulated PBMC to release TNF-alpha (P < 0.01). Translocation of HMGB1 from nucleus to cytosol was also found in infiltrated cells in gingival tissues from patients, and HMGB1 in GCF from patients was significantly higher than that from healthy people P < 0.01).
  
  
  CONCLUSIONS
  The results suggest that HMGB1 may play an important role in the pathological progress of chronic periodontitis.
authors.forename: Huaqiang
authors.initials: H
authors.lastname: Zhao
authors.forename: Pingping
authors.initials: P
authors.lastname: Mu
authors.forename: Lingling
authors.initials: L
authors.lastname: Wei
authors.forename: Meng
authors.initials: M
authors.lastname: Hou
authors.forename: Qinfeng
authors.initials: Q
authors.lastname: Sun
authors.forename: Hui
authors.initials: H
authors.lastname: Song
authors.forename: Pishan
authors.initials: P
authors.lastname: Yang
mesh_terms: D055113:Chronic Periodontitis; D005881:Gingiva; D024243:HMGB1 Protein; D006801:Humans; D007963:Leukocytes, Mononuclear; D008297:Male; D014409:Tumor Necrosis Factor-alpha
chemical_list: D024243:HMGB1 Protein; D014409:Tumor Necrosis Factor-alpha