title: Bacterial recognition of silicon nanowire arrays.
abstract: Understanding how living cells interact with nanostructures is integral to a better understanding of the fundamental principles of biology and the development of next-generation biomedical/bioenergy devices. Recent studies have demonstrated that mammalian cells can recognize nanoscale topographies and respond to these structures. From this perspective, there is a growing recognition that nanostructures, along with their specific physicochemical properties, can also be used to regulate the responses and motions of bacterial cells. Here, by utilizing a well-defined silicon nanowire array platform and single-cell imaging, we present direct evidence that Shewanella oneidensis MR-1 can recognize nanoscale structures and that their swimming patterns and initial attachment locations are strongly influenced by the presence of nanowires on a surface. Analyses of bacterial trajectories revealed that MR-1 cells exhibited a confined diffusion mode in the presence of nanowires and showed preferential attachment to the nanowires, whereas a superdiffusion mode was observed in the absence of nanowires. These results demonstrate that nanoscale topography can affect bacterial movement and attachment and play an important role during the early stages of biofilm formation.
authors.affiliation: Department of Chemistry, University of California, Berkeley, California 94720, USA.
authors.forename: Hoon Eui
authors.initials: HE
authors.lastname: Jeong
authors.forename: Ilsoo
authors.initials: I
authors.lastname: Kim
authors.forename: Pierre
authors.initials: P
authors.lastname: Karam
authors.forename: Heon-Jin
authors.initials: HJ
authors.lastname: Choi
authors.forename: Peidong
authors.initials: P
authors.lastname: Yang
mesh_terms: D018407:Bacterial Physiological Phenomena; D053770:Nanowires / Q000737:chemistry*; D012825:Silicon / Q000737:chemistry*; D059010:Single-Cell Analysis; D013499:Surface Properties
chemical_list: D012825:Silicon