title: A study of DNA protective effect of orange juice supplementation.
abstract: The potential acute genoprotective effect of orange juice supplementation was investigated. Six healthy subjects (aged 33 to 60 years; 3 women and 3 men) were asked to drink 400 mL of commercial orange juice, which contained 100 mg vitamin C and 40.8 g sugar. Venous blood (2 mL) was taken before and 2 h after ingestion (test trial). A week later, the subjects were asked to repeat the trial by drinking 400 mL water with 100 mg vitamin C and 40.8 g glucose (control trial). Lymphocytes isolated from blood samples underwent comet assay on the day of collection. Pre- and postingestion DNA damage scores were measured in both the test and control trials. Results showed that there was a significant decrease in DNA damage induced by hydrogen peroxide after 2 h of supplementation with orange juice, and no change in baseline DNA damage. There was no significant decrease in the DNA damage in lymphocytes in the control trial.
authors.affiliation: a Department of Applied Science, Hong Kong Institute of Vocational Education (Shatin), 21 Yuen Wo Road, Shatin, New Territories, Hong Kong; Macao Society for the Study of Women's Health, Macao.
authors.forename: Yim Tong
authors.initials: YT
authors.lastname: Szeto
authors.forename: Tai Lun
authors.initials: TL
authors.lastname: To
authors.forename: Sok Cheon
authors.initials: SC
authors.lastname: Pak
authors.forename: Wouter
authors.initials: W
authors.lastname: Kalle
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology; D001628:Beverages; D020552:Comet Assay; D004247:DNA; D004249:DNA Damage / Q000187:drug effects; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects
chemical_list: D000975:Antioxidants; D004247:DNA; D001205:Ascorbic Acid