title: Rapid electrochemical screening of NAD-dependent dehydrogenases in a 96-well format.
abstract: The electrochemical detection of dehydrogenase activity in crude cell lysates is performed simultaneously using 96 carbon electrodes modified with electrografted phenazines. The method is applied to the screening of a library of formate dehydrogenase mutants obtained by directed evolution.
authors.affiliation: GEMBAS, ICBMS UMR 5246, Université Lyon 1, CNRS, INSA Lyon, CPE Lyon, 43 bd du 11 Novembre 1918, 69622 Villeurbanne Cedex, France.
authors.forename: Sofiène
authors.initials: S
authors.lastname: Abdellaoui
authors.forename: Mourad
authors.initials: M
authors.lastname: Bekhouche
authors.forename: Alexandre
authors.initials: A
authors.lastname: Noiriel
authors.forename: Robert
authors.initials: R
authors.lastname: Henkens
authors.forename: Celia
authors.initials: C
authors.lastname: Bonaventura
authors.forename: Loïc J
authors.initials: LJ
authors.lastname: Blum
authors.forename: Bastien
authors.initials: B
authors.lastname: Doumèche
mesh_terms: D055162:Biocatalysis; D002175:Candida / Q000201:enzymology; D002244:Carbon / Q000737:chemistry; D019020:Directed Molecular Evolution; D055664:Electrochemical Techniques; D004566:Electrodes; D005560:Formate Dehydrogenases / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D009243:NAD / Q000737:chemistry*; D010619:Phenazines / Q000737:chemistry
chemical_list: D010619:Phenazines; D009243:NAD; D002244:Carbon; D005560:Formate Dehydrogenases