title: Splenic manifestations of chronic autoimmune disorder: a report of five cases with histiocytic necrotizing change in four cases.
abstract: OBJECTIVE
  Autoimmune diseases (AD) are associated with lymphadenopathy and splenomegaly. Changes in the spleen have not been characterized completely in AD; we describe splenectomy specimens from five patients with chronic AD, highlighting the presence of necrotizing histiocytosis.
  
  
  RESULTS
  Of the patients (three males and two females; mean 40 years), four had systemic lupus erythematosus; one had rheumatoid arthritis. All had moderate splenomegaly (213-803 g, mean 421 g). Four cases exhibited necrosis with apoptosis and karyorrhectic debris occurring in the white pulp and minimal acute inflammation; one showed florid follicular hyperplasia. Splenic involvement ranged from focal to extensive. Plasma cells were negative for IgG4. Haematoxylin bodies were not identified. Stains for infectious organisms were negative. Immunohistochemical studies showed that lymphocytes surrounding the necrosis were a mixture of CD4(+) and CD8(+) T cells; CD123-positive plasmacytoid dendritic cells were not present, and staining for kappa and lambda light chains showed no clonality. 16S rDNA PCR was performed; no amplification was seen in three of four cases tested for bacteria specific rDNA. Epstein-Barr virus-encoded RNA (EBER) in situ hybridization studies highlighted rare positive cells in four cases.
  
  
  CONCLUSIONS
  Splenomegaly in AD is thought to be hyperplasic, but we present four cases showing histiocytic necrosis, a finding which should be considered part of the spectrum of AD in the spleen.
authors.affiliation: Joint Pathology Center, Silver Spring, MD, USA.
authors.forename: Aaron
authors.initials: A
authors.lastname: Auerbach
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Summers
authors.forename: Binxue
authors.initials: B
authors.lastname: Zhang
authors.forename: Nadine S
authors.initials: NS
authors.lastname: Aguilera
mesh_terms: D000328:Adult; D001172:Arthritis, Rheumatoid / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002908:Chronic Disease; D003713:Dendritic Cells / Q000378:metabolism / Q000473:pathology; D005260:Female; D004854:Herpesvirus 4, Human / Q000235:genetics; D020042:Histiocytic Necrotizing Lymphadenitis / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D008180:Lupus Erythematosus, Systemic / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D013154:Spleen / Q000378:metabolism / Q000473:pathology