title: Bone morphogenetic protein-5 and early endothelial outgrowth cells (eEOCs) in acute ischemic kidney injury (AKI) and 5/6-chronic kidney disease.
abstract: Early endothelial outgrowth cells (eEOCs) reproducibly have been shown to act protectively in acute ischemic kidney injury (AKI) and chronic kidney injury. Bone morphogenetic protein-5 (BMP-5) acted antifibrotically in human hypertensive nephropathy. The aim of the current study was to analyze effects of BMP-5 treatment in an eEOC-based therapy of murine AKI and 5/6-nephrectomy. Male C57/Bl6N mice were either subjected to unilateral renal artery clamping postuninephrectomy or to 5/6-nephrectomy. Untreated or BMP-5-pretreated murine eEOCs were injected into recipient animals at the time of reperfusion (AKI) or at 2 and 5 days after 5/6-nephrectomy. Analysis of renal function and morphology was performed at 48 h and at 6 wk (AKI) or at 8 wk (5/6 model). Cellular consequences of eEOC treatment were evaluated using different in vitro assays. AKI was mitigated significantly by injecting BMP-5-pretreated eEOCs. Renal function was improved at 48 h [corrected] after cell therapy. In 5/6-nephrectomy, the cells failed to act renoprotectively, [corrected] but proteinuria was reduced after administering untreated eEOCs." Next, the original version read as "BMP-5 acts as a potent eEOC agonist in murine AKI in the short [corrected] term. Cell effects in 5/6-nephrectomy are heterogenous, but untreated cells act antifibrotically [corrected] without any impact on EnMT.
authors.affiliation: Department of Nephrology and Rheumatology, University Hospital of Göttingen, Göttingen, Germany. d.patschan@gmail.com
authors.forename: Daniel
authors.initials: D
authors.lastname: Patschan
authors.forename: Katrin
authors.initials: K
authors.lastname: Schwarze
authors.forename: Andrea
authors.initials: A
authors.lastname: Lange
authors.forename: Nyree
authors.initials: N
authors.lastname: Meise
authors.forename: Elvira
authors.initials: E
authors.lastname: Henze
authors.forename: Jan Ulrich
authors.initials: JU
authors.lastname: Becker
authors.forename: Susann
authors.initials: S
authors.lastname: Patschan
authors.forename: Gerhard A
authors.initials: GA
authors.lastname: Müller
mesh_terms: D058186:Acute Kidney Injury / Q000473:pathology*; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D055417:Bone Morphogenetic Protein 5 / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology; D018836:Inflammation Mediators / Q000502:physiology; D007677:Kidney Function Tests; D051379:Mice; D008810:Mice, Inbred C57BL; D008856:Microscopy, Fluorescence; D009336:Necrosis; D009392:Nephrectomy; D011507:Proteinuria / Q000378:metabolism; D051436:Renal Insufficiency, Chronic / Q000473:pathology*
chemical_list: D055417:Bone Morphogenetic Protein 5; D018836:Inflammation Mediators
keywords: BMP-5; EPC; acute renal failure