title: Calcium imaging of the cyclic nucleotide response.
abstract: Calcium (Ca(2+)) is a key component of the signalling network by which plant cells respond to developmental and environmental signals. A change in guard cell cytosolic free Ca(2+)([Ca(2+)]cyt) is an early event in the response of stomata to both opening and closing stimuli, and cyclic nucleotide-mediated Ca(2+) signalling has been implicated in the regulation of stomatal aperture. A range of techniques have been used to measure [Ca(2+)]cyt in plant cells. Here we describe a potential method for imaging cyclic nucleotide-induced changes in [Ca(2+)]cyt in guard cells using the cameleon ratiometric Ca(2+) reporter protein.
authors.affiliation: Lancaster Environment Centre, Lancaster University, Lancaster, UK.
authors.forename: Martin R
authors.initials: MR
authors.lastname: McAinsh
authors.forename: Stephen K
authors.initials: SK
authors.lastname: Roberts
authors.forename: Lyudmila V
authors.initials: LV
authors.lastname: Dubovskaya
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D020013:Calcium Signaling; D002138:Calibration; D005453:Fluorescence; D021621:Imaging, Three-Dimensional / Q000379:methods*; D057054:Molecular Imaging; D009712:Nucleotides, Cyclic / Q000378:metabolism*; D010477:Perfusion; D019441:Plant Epidermis / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism
chemical_list: D009712:Nucleotides, Cyclic