title: An environmentally sensitive fluorescent purine nucleoside that changes emission wavelength upon hybridization.
abstract: C7-naphthylethynylated 8-aza-7-deaza-2'-deoxyguanosine (na)G was synthesized and its photophysical properties were examined. The fluorescent nucleoside exhibited solvatofluorochromic properties (Δλ(fl)(max) = 67 nm). An ODN probe containing (na)G forms a stable base pair only with C and discriminates structural changes such as mismatches and deletions by a distinct change in its emission wavelength.
authors.affiliation: Department of Chemical Biology and Applied Chemistry, College of Engineering, Nihon University, Koriyama, Fukushima 963-8642, Japan. saitoy@chem.ce.nihon-u.ac.jp
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Saito
authors.forename: Azusa
authors.initials: A
authors.lastname: Suzuki
authors.forename: Yuji
authors.initials: Y
authors.lastname: Okada
authors.forename: Yuki
authors.initials: Y
authors.lastname: Yamasaka
authors.forename: Nobukatsu
authors.initials: N
authors.lastname: Nemoto
authors.forename: Isao
authors.initials: I
authors.lastname: Saito
mesh_terms: D004252:DNA Mutational Analysis; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D009154:Mutation; D009693:Nucleic Acid Hybridization; D015345:Oligonucleotide Probes / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D003849:Deoxyguanosine