title: Genetic characterization of moaB mutants of Escherichia coli.
abstract: The moaABCDE operon of Escherichia coli encodes enzymes essential for the biosynthesis of the molybdenum cofactor (Moco). However, the role of the moaB gene within this operon has remained enigmatic. Here, we have investigated the effect of moaB defects on two phenotypes diagnostic for Moco-deficiency: chlorate-resistance and sensitivity to the base analog 6-N-hydroxylaminopurine (HAP). We found that transposon insertions in moaB caused partial Moco-deficiency associated with chlorate-resistance, but not for HAP-sensitivity. On the other hand, in-frame deletions of moaB, or moaB overexpression, had no effect on either phenotype. Our combined data are consistent with the lack of any role for MoaB in Moco biosynthesis in E. coli.
authors.affiliation: Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA. stanislav.kozmin@duke.edu
authors.forename: Stanislav G
authors.initials: SG
authors.lastname: Kozmin
authors.forename: Roel M
authors.initials: RM
authors.lastname: Schaaper
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives* / Q000494:pharmacology; D002704:Chlorates / Q000494:pharmacology*; D003067:Coenzymes / Q000096:biosynthesis; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D008667:Metalloproteins / Q000096:biosynthesis; D000090006:Molybdenum Cofactors; D016254:Mutagenesis, Insertional; D011621:Pteridines
chemical_list: D000225:Adenine; D002704:Chlorates; D003067:Coenzymes; D029968:Escherichia coli Proteins; D008667:Metalloproteins; D000090006:Molybdenum Cofactors; D011621:Pteridines; C492989:MoaB protein, E coli; C032610:6-N-hydroxylaminopurine; C022261:molybdopterin
keywords: 6-N-Hydroxylaminopurine; Chlorate resistance; Molybdenum cofactor; moaABCDE operon; mogA gene