title: Jejudonia soesokkakensis gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from the junction between the ocean and a freshwater spring, and emended description of the genus Aureitalea Park et al. 2012.
abstract: A Gram-negative, non-flagellated, non-gliding and rod-shaped bacterial strain, designated SSK1-1(T), was isolated from the junction between the ocean and a freshwater spring at Jeju island of South Korea. Strain SSK1-1(T) was found to grow optimally at 30 °C, at pH 7.0-7.5 and in the presence of 2 % (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain SSK1-1(T) is phylogenetically most closely related to members of the genera Ulvibacter and Aureitalea, with which it exhibited 16S rRNA gene sequence similarities of 93.1-95.3 %. The results of chemotaxonomic analyses distinguish strain SSK1-1(T) from the genera Ulvibacter and Aureitalea. Strain SSK1-1(T) was determined to contain MK-6 as the predominant menaquinone and iso-C15:0, anteiso-C15:0 and iso-C16:0 as the major fatty acids. The major polar lipids of strain SSK1-1(T) were identified as phosphatidylethanolamine and one unidentified lipid. The DNA G + C content of strain SSK1-1(T) was determined to be 39.9 mol%. The phylogenetic analysis, chemotaxonomic data and other phenotypic properties revealed that the strain SSK1-1(T) can be distinguished from the members of previously known genera of the family Flavobacteriaceae. On the basis of the data presented, strain SSK1-1(T) is considered to represent a novel genus and species, for which the name Jejudonia soesokkakensis gen. nov., sp. nov. is proposed. The type strain is SSK1-1(T) (=KCTC 32325(T) = CCUG 63830(T)). An emended description of the genus Aureitalea is also proposed.
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.
authors.forename: Sooyeon
authors.initials: S
authors.lastname: Park
authors.forename: Jung-Sook
authors.initials: JS
authors.lastname: Lee
authors.forename: Kenu-Chul
authors.initials: KC
authors.lastname: Lee
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001482:Base Composition; D001483:Base Sequence; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D041965:Flavobacteriaceae / Q000145:classification / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D005618:Fresh Water / Q000382:microbiology; D006863:Hydrogen-Ion Concentration; D008055:Lipids / Q000032:analysis; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D060985:Natural Springs / Q000382:microbiology*; D010641:Phenotype; D010802:Phylogeny; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D013045:Species Specificity; D013696:Temperature; D024482:Vitamin K 2 / Q000032:analysis; D014871:Water Microbiology
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D005227:Fatty Acids; D008055:Lipids; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2