title: Decreased expression of miR-430 promotes the development of bladder cancer via the upregulation of CXCR7.
abstract: MicroRNAs (miRNAs) have been demonstrated to be involved in the development of numerous types of malignant tumor. However, the role of miRNA-430 (miR-430) in bladder cancer remains unclear. In the present study, we observed that the expression of miR-430 was significantly downregulated in bladder cancer. Furthermore, the overexpression of miR-430 in human bladder cancer 5637 cells significantly inhibited cell proliferation, migration and colony formation efficiency. These findings were contrary to those obtained following the overexpression of CXCR7, which was found to be a direct target of miR-430 in this study. Further analysis showed that cell proliferation- and migration-related genes, including ERK, matrix metalloproteinase-2 (MMP-2) and MMP-9, were significantly downregulated in miR-430 overexpressed 5637 cells, while they were markedly upregulated in CXCR7 overexpressed 5637 cells. In conclusion, our study reveals important roles of miR-430 and CXCR7 in bladder cancer, and suggests that the downregulation of miR-430 enhances the development of bladder cancer, partly via the upregulation of CXCR7.
authors.affiliation: Department of Urology, Second Xiangya Hospital of Central South University, Changsha, Hunan, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Zhao
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhu
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Zhong
authors.forename: Ran
authors.initials: R
authors.lastname: Xu
authors.forename: Zijian
authors.initials: Z
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Cao
authors.forename: Yi
authors.initials: Y
authors.lastname: Hou
mesh_terms: D002453:Cell Cycle / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D034622:RNA Interference; D054387:Receptors, CXCR / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D014410:Tumor Stem Cell Assay; D001743:Urinary Bladder / Q000378:metabolism; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000378:metabolism
chemical_list: C501896:ACKR3 protein, human; C585431:MIRN430 microRNA, human; D035683:MicroRNAs; D054387:Receptors, CXCR