title: Acute and subchronic dermal toxicity of Vitex negundo essential oil.
abstract: Vitex negundo is a common herb in different herbal formulation. The potential acute and sub-chronic dermal toxicities were evaluated as per OECD (Organization for Economic Cooperation and Development) guidelines 402 and 411, respectively. Both sexes of Wistar rats were exposed to Vitex negundo oil of 2000 mg/kg body weight for acute dermal toxicity, whereas in the dermal sub-chronic toxicity study, rats were exposed to Vitex negundo oil 250, 500 and 1000 mg/kg body weight, respectively, for five times a week for 90 d. In acute and sub-chronic toxicity studies, all animals were normal without any behavioral, serum biochemistry, hematology, necroscopical and histopathological changes. The no observed effect level (NOEL) and no observed adverse effect level (NOAEL) of Vitex negundo oil were 250 and 1000 mg/kg/day, respectively. Vitex negundo oil is under the category 5 (Unclassified) according to the Globally Harmonized System, with an LD50 value of over 2000 mg/kg.
authors.affiliation: Division of Pharmaceutical Technology and.
authors.forename: Pronobesh
authors.initials: P
authors.lastname: Chattopadhyay
authors.forename: Subham
authors.initials: S
authors.lastname: Banerjee
authors.forename: Manash Pratim
authors.initials: MP
authors.lastname: Pathak
authors.forename: Amit
authors.initials: A
authors.lastname: Agnihotri
authors.forename: Sanjeev
authors.initials: S
authors.lastname: Karmakar
authors.forename: Danswrang
authors.initials: D
authors.lastname: Goyary
authors.forename: Sunil
authors.initials: S
authors.lastname: Dhiman
authors.forename: Vijay
authors.initials: V
authors.lastname: Veer
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D007928:Lethal Dose 50; D008297:Male; D019055:No-Observed-Adverse-Effect Level; D009822:Oils, Volatile / Q000302:isolation & purification / Q000633:toxicity*; D010938:Plant Oils / Q000302:isolation & purification / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D023382:Toxicity Tests, Acute; D059550:Toxicity Tests, Subchronic; D020950:Vitex / Q000737:chemistry*
chemical_list: D009822:Oils, Volatile; D010938:Plant Oils