title: Toward embedded laboratory automation for smart Lab-on-a-Chip embryo arrays.
abstract: Lab-on-a-Chip (LOC) biomicrofluidic technologies are rapidly emerging bioanalytical tools that can miniaturize and revolutionize in situ research on embryos of small vertebrate model organisms such as zebrafish (Danio rerio) and clawed African frog (Xenopus laevis). Despite considerable progress being made in fabrication techniques of chip-based devices, they usually still require excessive and manual actuation and data acquisition that significantly reduce throughput and introduce operator-related analytical bias. This work describes the development of a proof-of-concept embedded platform that integrates an innovative LOC zebrafish embryo array technology with an electronic interface to provide higher levels of laboratory automation for in situ biotests. The integrated platform was designed to perform automatic immobilization, culture and treatment of developing zebrafish embryos during fish embryo toxicity (FET) biotests. The system was equipped with a stepper motor driven stage, solenoid-actuated pinch valves, miniaturized peristaltic pumps as well as Peltier heating module. Furthermore, a Field Programmable Gate Array (FPGA) was used to implement an embedded hardware/software solution and interface to enable real-time control over embryo loading and immobilization; accurate microfluidic flow control; temperature stabilization and also automatic time-resolved image acquisition of developing zebrafish embryos. This work presents evidence that integration of embedded electronic interfaces with microfluidic chip-based technologies can bring the Lab-on-a-Chip a step closer to fully automated analytical systems.
authors.affiliation: Department of Electrical and Computer Engineering, University of Auckland, Auckland, New Zealand.
authors.forename: Kevin I-Kai
authors.initials: KI
authors.lastname: Wang
authors.forename: Zoran
authors.initials: Z
authors.lastname: Salcic
authors.forename: Johnny
authors.initials: J
authors.lastname: Yeh
authors.forename: Jin
authors.initials: J
authors.lastname: Akagi
authors.forename: Feng
authors.initials: F
authors.lastname: Zhu
authors.forename: Chris J
authors.initials: CJ
authors.lastname: Hall
authors.forename: Kathryn E
authors.initials: KE
authors.lastname: Crosier
authors.forename: Philip S
authors.initials: PS
authors.lastname: Crosier
authors.forename: Donald
authors.initials: D
authors.lastname: Wlodkowic
mesh_terms: D000818:Animals; D004867:Equipment Design; D007091:Image Processing, Computer-Assisted; D056656:Lab-On-A-Chip Devices; D046888:Tissue Array Analysis / Q000295:instrumentation*; D046509:Tissue Culture Techniques / Q000295:instrumentation; D018675:Toxicity Tests / Q000295:instrumentation*; D015027:Zebrafish / Q000196:embryology*