title: Effects of Y27632 on keratinocyte procurement and wound healing.
abstract: A number of Rho-kinase inhibitors have been developed for various clinical applications. We examined the effects of the Rho-kinase inhibitor Y27632 on keratinocyte proliferation and migration, and found that it promoted primary human keratinocyte proliferation and migration in both monolayer and skin explant cultures. In addition, topical application of Y27632 enhanced cutaneous wound closure in the majority of wounds in mice. The growth and migration effects of Y27632 appeared to be specific to keratinocytes compared with dermal fibroblasts, and required intact Jun kinase function. Y27632 seems to be a promising agent for keratinocyte procurement and wounding healing.
authors.affiliation: Department of Dermatology, Duke University, Durham, NC, USA.
authors.forename: V D
authors.initials: VD
authors.lastname: Gandham
authors.forename: R L
authors.initials: RL
authors.lastname: Maddala
authors.forename: V
authors.initials: V
authors.lastname: Rao
authors.forename: J Y
authors.initials: JY
authors.lastname: Jin
authors.forename: D L
authors.initials: DL
authors.lastname: Epstein
authors.forename: R P
authors.initials: RP
authors.lastname: Hall
authors.forename: J Y
authors.initials: JY
authors.lastname: Zhang
mesh_terms: D000287:Administration, Topical; D000577:Amides / Q000494:pharmacology*; D000818:Animals; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004195:Disease Models, Animal; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects*; D051379:Mice; D011725:Pyridines / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects*; D054460:rho-Associated Kinases / Q000037:antagonists & inhibitors*
chemical_list: D000577:Amides; D004791:Enzyme Inhibitors; D011725:Pyridines; C108830:Y 27632; D054460:rho-Associated Kinases