title: New isoflavones with cytotoxic activity from the rhizomes of Iris germanica L.
abstract: Two new compounds, 5-methoxy-3',4'-dihydroxy-6,7-methylenedioxy-4H-1-benzo-pyran-4-one(iriskashmirianin A) (1) and 5,3'-dihydroxy-3-(4'-β-D-glucopyranosyl)-6,7-methylenedioxy-4H-1-benzo-pyran-4-one (germanaism H) (2), along with eight known compounds (3-10), were isolated from the rhizomes of Iris germanica L. The cytotoxicities of these compounds were tested using Ehrlich's ascites carcinoma (EAC) cancer cell line by 3-(4, 5-dimethylthiazole-2-yl)-2, 5-diphenyltetrazoli-umbromide (MTT) and ATP assays. The results showed that these compounds possessed antiproliferative effects on EAC cell line. Among them, compound 1 possessed the best cytotoxic activity with IC50 ± SD of 20.9 ± 2.7 and 4.3 ± 0.9 μM for MTT and ATP assay methods, respectively.
authors.affiliation: a Department of Resources Science of Traditional Chinese Medicines , State Key Laboratory of Natural Medicines, China Pharmaceutical University , Nanjing , 210009 , P.R. China.
authors.forename: Guo-Yong
authors.initials: GY
authors.lastname: Xie
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Qin
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.forename: Bin-Bin
authors.initials: BB
authors.lastname: Lin
authors.forename: Guo-Kai
authors.initials: GK
authors.lastname: Wang
authors.forename: Guang-Kai
authors.initials: GK
authors.lastname: Xu
authors.forename: Rui
authors.initials: R
authors.lastname: Wen
authors.forename: Min-Jian
authors.initials: MJ
authors.lastname: Qin
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D007498:Iris / Q000737:chemistry*; D007529:Isoflavones / Q000302:isolation & purification / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D027343:Rhizome / Q000737:chemistry*; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D007529:Isoflavones