title: Swine, human or avian influenza viruses differentially activates porcine dendritic cells cytokine profile.
abstract: Swine influenza virus (SwIV) is considered a zoonosis and the fact that swine may act as an intermediate reservoir for avian influenza virus, potentially infectious for humans, highlights its relevance and the need to understand the interaction of different influenza viruses with the porcine immune system. Thus, in vitro porcine bone marrow-derived dendritic cell (poBMDCs) were infected with a circulating SwIV A/Swine/Spain/SF32071/2007(H3N2), 2009 human pandemic influenza virus A/Catalonia/63/2009(H1N1), low pathogenic avian influenza virus (LPAIV) A/Anas plathyrhynchos/Spain/1877/2009(aH7N2) or high pathogenic avian influenza virus (HPAIV) A/Chicken/Italy/5093/1999(aH7N1). Swine influenza virus H3N2 infection induced an increase of SLA-I and CD80/86 at 16 and 24h post infection (hpi), whereas the other viruses did not. All viruses induced gene expression of NF-κB, TGF-β, IFN-β and IL-10 at the mRNA level in swine poBMDCs to different extents and in a time-dependent manner. All viruses induced the secretion of IL-12 mostly at 24hpi whereas IL-18 was detected at all tested times. Only swH3N2 induced IFN-α in a time-dependent manner. Swine H3N2, aH7N2 and aH7N1 induced secretion of TNF-α also in a time-dependent manner. Inhibition of NF-κB resulted in a decrease of IFN-α and IL-12 secretion by swH3N2-infected poBMDC at 24hpi, suggesting a role of this transcription factor in the synthesis of these cytokines. Altogether, these data might help in understanding the relationship between influenza viruses and porcine dendritic cells in the innate immune response in swine controlled through soluble mediators and transcription factors.
authors.affiliation: Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra (Cerdanyola del Vallès), Spain.
authors.forename: Tufária
authors.initials: T
authors.lastname: Mussá
authors.forename: Maria
authors.initials: M
authors.lastname: Ballester
authors.forename: Erika
authors.initials: E
authors.lastname: Silva-Campa
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Baratelli
authors.forename: Núria
authors.initials: N
authors.lastname: Busquets
authors.forename: Marie-Pier
authors.initials: MP
authors.lastname: Lecours
authors.forename: Javier
authors.initials: J
authors.lastname: Dominguez
authors.forename: Massimo
authors.initials: M
authors.lastname: Amadori
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Fraile
authors.forename: Jesús
authors.initials: J
authors.lastname: Hernández
authors.forename: María
authors.initials: M
authors.lastname: Montoya
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D003713:Dendritic Cells / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000276:immunology; D006801:Humans; D009980:Influenza A virus / Q000276:immunology*; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D013552:Swine / Q000276:immunology*; D059467:Transcriptome
chemical_list: D016207:Cytokines; D011956:Receptors, Cell Surface; C092488:sialic acid receptor