title: High performance enzyme fuel cells using a genetically expressed FAD-dependent glucose dehydrogenase α-subunit of Burkholderia cepacia immobilized in a carbon nanotube electrode for low glucose conditions.
abstract: FAD-dependent glucose dehydrogenase (FAD-GDH) of Burkholderia cepacia was successfully expressed in Escherichia coli and subsequently purified in order to use it as an anode catalyst for enzyme fuel cells. The purified enzyme has a low Km value (high affinity) towards glucose, which is 463.8 μM, up to 2-fold exponential range lower compared to glucose oxidase. The heterogeneous electron transfer coefficient (Ks) of FAD-GDH-menadione on a glassy carbon electrode was 10.73 s(-1), which is 3-fold higher than that of GOX-menadione, 3.68 s(-1). FAD-GDH was able to maintain its native glucose affinity during immobilization in the carbon nanotube and operation of enzyme fuel cells. FAD-GDH-menadione showed 3-fold higher power density, 799.4 ± 51.44 μW cm(-2), than the GOX-menadione system, 308.03 ± 17.93 μW cm(-2), under low glucose concentration, 5 mM, which is the concentration in normal physiological fluid.
authors.affiliation: School of Environmental Science and Engineering, Gwangju Institute of Science and Technology (GIST), Buk-gu, Gwangju, Republic of Korea.
authors.forename: Deby
authors.initials: D
authors.lastname: Fapyane
authors.forename: Soo-Jin
authors.initials: SJ
authors.lastname: Lee
authors.forename: Seo-Hee
authors.initials: SH
authors.lastname: Kang
authors.forename: Du-Hyun
authors.initials: DH
authors.lastname: Lim
authors.forename: Kwon-Koo
authors.initials: KK
authors.lastname: Cho
authors.forename: Tae-hyun
authors.initials: TH
authors.lastname: Nam
authors.forename: Jae-Pyoung
authors.initials: JP
authors.lastname: Ahn
authors.forename: Jou-Hyeon
authors.initials: JH
authors.lastname: Ahn
authors.forename: Seon-Won
authors.initials: SW
authors.lastname: Kim
authors.forename: In Seop
authors.initials: IS
authors.lastname: Chang
mesh_terms: D016956:Burkholderia cepacia / Q000201:enzymology*; D020134:Catalytic Domain; D055664:Electrochemical Techniques; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D042843:Glucose 1-Dehydrogenase / Q000737:chemistry / Q000378:metabolism*; D007700:Kinetics; D037742:Nanotubes, Carbon / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D042843:Glucose 1-Dehydrogenase; D005947:Glucose