title: Rapid detection of human cytomegalovirus UL97 and UL54 mutations for antiviral resistance in clinical specimens.
abstract: Drug-resistant cytomegalovirus appears during prolonged anti-cytomegalovirus therapy. Assays for human cytomegalovirus viral protein kinase (UL97) and viral DNA polymerase (UL54) gene mutations conferring drug resistance have been used rather than susceptibility assays to assess clinical specimens. In this study a sensitive system for genotype assay of UL97 and UL54 in clinical specimens with as few as six copies/µg of DNA was developed.
authors.affiliation: Department of Virology, University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Daikoku
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Saito
authors.forename: Takamitsu
authors.initials: T
authors.lastname: Aihara
authors.forename: Masahiro
authors.initials: M
authors.lastname: Ikeda
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Hiroki
authors.initials: H
authors.lastname: Hosoi
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Nishida
authors.forename: Masaya
authors.initials: M
authors.lastname: Takemoto
authors.forename: Kimiyasu
authors.initials: K
authors.lastname: Shiraki
mesh_terms: D000328:Adult; D000998:Antiviral Agents / Q000494:pharmacology*; D002648:Child; D003587:Cytomegalovirus / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000821:virology; D004259:DNA-Directed DNA Polymerase / Q000235:genetics*; D024882:Drug Resistance, Viral; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D008875:Middle Aged; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D020125:Mutation, Missense; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics*; D012680:Sensitivity and Specificity; D014764:Viral Proteins / Q000235:genetics*
chemical_list: D000998:Antiviral Agents; C109367:UL54 protein, Human herpesvirus 5; D014764:Viral Proteins; D017853:Phosphotransferases (Alcohol Group Acceptor); C075365:ganciclovir kinase; D004259:DNA-Directed DNA Polymerase