title: A new strategy based on cholesterol-functionalized iron oxide magnetic nanoparticles for determination of polycyclic aromatic hydrocarbons by high-performance liquid chromatography with cholesterol column.
abstract: This study reported for the first time the use of cholesterol-functionalized magnetic nanoparticles (Fe3O4@SiO2@Chol) for the determination of polycyclic aromatic hydrocarbons (PAHs) in traditional Chinese medicine samples (TCMs) by high performance liquid chromatography (HPLC) coupled with fluorescence detection. The method was efficient, environmentally friendly, and fast. The solvent consumption of the proposed column is only half of the conventional column but with higher efficiency. Influencing factors, including sorbent amount, desorption solvent, sample volume and extraction time, were investigated in detail. Under the optimum conditions, good linearity (R(2)>0.991) was obtained over the range of 5-400 ng g(-1), with limits of detection (LOD) 0.75, 0.50, 1.0, 0.56, 0.60, 0.84 and 0.80 ng g(-1) for anthracene, fluoranthene, pyrene, chrysene, benzo[a]anthracene, benzo(b)fluoranthene and benzo(k)fluoranthene, respectively.
authors.affiliation: State Key Laboratory of Chemo/Biosensing & Chemometrics, College of Chemistry & Chemical Engineering, Hunan University, Changsha 410082, China.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Yan
authors.forename: Jinbin
authors.initials: J
authors.lastname: Yuan
authors.forename: Genhua
authors.initials: G
authors.lastname: Zhu
authors.forename: Ying
authors.initials: Y
authors.lastname: Zou
authors.forename: Chunyan
authors.initials: C
authors.lastname: Chen
authors.forename: Shaolei
authors.initials: S
authors.lastname: Yang
authors.forename: Shouzhuo
authors.initials: S
authors.lastname: Yao
mesh_terms: D002784:Cholesterol / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005290:Ferric Compounds / Q000737:chemistry*; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D010316:Particle Size; D011084:Polycyclic Aromatic Hydrocarbons / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D005290:Ferric Compounds; D011084:Polycyclic Aromatic Hydrocarbons; C000499:ferric oxide; D002784:Cholesterol