title: MicroRNA-9 enhances migration and invasion through KLF17 in hepatocellular carcinoma.
abstract: Metastasis is one of the hallmarks of cancer malignancy that usually causes more detrimental effects than a primary tumor. Many microRNAs were reported to be involved in the process of tumor metastasis. Hep11 and Hep12 cells were derived from primary and recurrence (intrahepatic metastatic) sites of hepatocellular carcinoma (HCC), respectively. Hep12 exhibited a higher invasive and migratory potential than Hep11. There was also a significantly higher expression of miR-9 in Hep12 cells than in Hep11 cells. Further studies in HCC cell lines demonstrated that miR-9 could promote tumor cell migration and invasion. In addition, miR-9 downregulated KLF17 protein expression by targeting the 3'UTR region of the KLF17 gene directly. As a transcription factor, KLF17 directly acted on the promoters of EMT-related genes (ZO-1, Vimentin and Fibronectin (FN)) in HCC cell lines. Therefore, we conclude that miR-9 may possibly promote HCC migration and invasion through regulation of KLF17.
authors.affiliation: Department of Oncology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, People's Republic of China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Sun
authors.forename: Qin
authors.initials: Q
authors.lastname: Han
authors.forename: Na
authors.initials: N
authors.lastname: Zhou
authors.forename: Shihua
authors.initials: S
authors.lastname: Wang
authors.forename: Shan
authors.initials: S
authors.lastname: Lu
authors.forename: Chunmei
authors.initials: C
authors.lastname: Bai
authors.forename: Robert Chunhua
authors.initials: RC
authors.lastname: Zhao
mesh_terms: D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics / Q000502:physiology; D047369:Chromatin Immunoprecipitation; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics*; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D011485:Protein Binding; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: C510638:KLF17 protein, human; C541874:MIRN92 microRNA, human; D035683:MicroRNAs; D014157:Transcription Factors
keywords: CHIP; E-ca; E-cadherin; EMT; FBS; FN; HBV; HCC; HNSCC; Hepatitis B virus; Hepatocellular carcinoma; Invasion; KLF-17; KLF17; Krüppel-like factor 17; MT; Vim; WT; chromatin immunoprecipitation; epithelial mesenchymal transition; fetal calf serum; fibronectin; head and neck squamous cell carcinoma; hepatocellular carcinoma; microRNA-9; mutant type; qRT-PCR; quantitative reverse transcription polymerase chain reaction; vimentin; wild type