title: Statistical optimization of medium components for milk-clotting enzyme production by Bacillus amyloliquefaciens D4 using wheat Bran-an agro-industry waste.
abstract: In this paper, two statistical methods were applied to optimize medium components to improve the production of the milk-clotting enzyme by Bacillus amyloliquefaciens D4. First, wheat bran juice, skim milk powder, and Na2HPO4 were shown to have significant effects on D4 enzyme production using the Plackett-Burman experimental design. Subsequently, an optimal medium was obtained using the Box-Behnken method, which consisted of 3.31 g/l of skim milk powder, 5.0 g/l of sucrose, 0.1 g/l of FeSO4·7H2O, 0.1 g/l of MgSO4·7H2O, 0.1 g/l of MnSO4·2H2O, 0.1 g/l of ZnSO4·7H2O, 1.52 g/l of Na2HPO4, and 172.45 g/l of wheat bran juice. With this optimal medium, the milk-clotting enzyme production was remarkably enhanced. The milk-clotting enzyme activity reached 3,326.7 SU/ml after incubation of 48 h, which was 1.76-fold higher than that of the basic medium, showing that the Plackett-Burman design and Box-Behnken response surface method are effective to optimize medium components, and B. amyloliquefaciens D4 possessed a high rennet-producing capacity in the optimal medium.
authors.affiliation: College of Food Science and Technology Engineering, Gansu Agricultural University, Lanzhou 730070, China.
authors.forename: Weibing
authors.initials: W
authors.lastname: Zhang
authors.forename: Xiaoling
authors.initials: X
authors.lastname: He
authors.forename: Hongna
authors.initials: H
authors.lastname: Liu
authors.forename: Huiyuan
authors.initials: H
authors.lastname: Guo
authors.forename: Fazheng
authors.initials: F
authors.lastname: Ren
authors.forename: Weidong
authors.initials: W
authors.lastname: Gao
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Wen
mesh_terms: D016282:Aspartic Acid Endopeptidases / Q000378:metabolism*; D001407:Bacillus / Q000201:enzymology* / Q000254:growth & development; D001709:Biotechnology / Q000379:methods; D003470:Culture Media / Q000737:chemistry*; D004043:Dietary Fiber / Q000378:metabolism*; D007220:Industrial Waste; D013997:Time Factors
chemical_list: D003470:Culture Media; D004043:Dietary Fiber; D007220:Industrial Waste; D016282:Aspartic Acid Endopeptidases; C022517:rennin-like enzyme (Aspergillus ochraceus)