title: [Change in dissolution of chemical components of frankincense-myrrh before and after their compatibility and effect on no release of LPS-induced macrophage cells].
abstract: OBJECTIVE
  To analyze the difference of chemical compounds of frankincense-myrrh before and after their compatibility, and evaluate the effect of differentiated compounds on NO generated by LPS-induced peritoneal macrophage cells in rats, in order to discuss synergetic material basis of frankincense-myrrh compatibility from the prospective of change in chemical constituents.
  
  
  METHODS
  UPLC-Q-TOF-MS/MS combined technology was used to analyze the chemical components of frankincense-myrrh before and after their compatibility. MarkerLynx 4. 1 statistical software was used to analyze differentiated compounds before and after their compatibility.
  
  
  RESULTS
  The results of PCA showed that there were significant differences in the combined extracts of frankincense-myrrh and the chromatogram of their combined liquid, suggesting significant differences in their chemical compounds before and after their compatibility; after their compatibility, the dissolution of pentacyclic triterpenoid (alpha-boswellic acid, beta-boswellic acid) and tetracyclic triterpenoid (elemonic acid, 3-acetoxy-16-hydroxy-dammar-24-ene, 3-hydroxytirucalla-8,24-dien-21-oic acid or 3-hydroxytirucalla-7,24-dien-21-oic acid) increased notably, while the dissolution of both yclic sesquiterpenes and macrocyclic diterpenoids decreased. According to the evaluation on in vitro activity, 2-methoxy-8, 12-epoxy-germa-1 (10), 7, 11-triene-6-ketone, 2-methoxy-5-acetoxyl-furan-germa-1 (10)-alkene-6-ketone and 3-carbonyl Euphorbia kansui-8, 24-diene-21-carboxylic acid notably inhibited NO generated by LPS-induced peritoneal macrophage cells in rats.
  
  
  CONCLUSIONS
  These findings provide scientific basis and reference for studies on anti-inflammatory material basis of frankincense-myrrh compatibility.
authors.affiliation: Key Laboratory for Traditional Chinese Medicine Formulae Research of Jiangsu Province, Nanjing University of Chinese Medicine, Nanjing 210046, China. chenting8409@126.com
authors.forename: Ting
authors.initials: T
authors.lastname: Chen
authors.forename: Shu-Lan
authors.initials: SL
authors.lastname: Su
authors.forename: Jin-Ao
authors.initials: JA
authors.lastname: Duan
authors.forename: Er-Xin
authors.initials: EX
authors.lastname: Shang
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Qian
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Tang
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry* / Q000302:isolation & purification; D029743:Boswellia / Q000737:chemistry*; D002853:Chromatography, Liquid; D029744:Commiphora / Q000737:chemistry*; D004357:Drug Synergism; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D008070:Lipopolysaccharides / Q000494:pharmacology; D017737:Macrophages, Peritoneal / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015394:Molecular Structure; D009569:Nitric Oxide / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D013729:Terpenes / Q000737:chemistry* / Q000302:isolation & purification; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D004365:Drugs, Chinese Herbal; D008070:Lipopolysaccharides; D013729:Terpenes; D014315:Triterpenes; D009569:Nitric Oxide; C054625:boswellic acid; C014119:myrrh oil