title: Plasmonic nanoprobes for intracellular sensing and imaging.
abstract: Recent advances in integrating nanotechnology and optical microscopy offer great potential in intracellular applications with improved molecular information and higher resolution. Continuous efforts in designing nanoparticles with strong and tunable plasmon resonance have led to new developments in biosensing and bioimaging, using surface-enhanced Raman scattering and two-photon photoluminescence. We provide an overview of the nanoprobe design updates, such as controlling the nanoparticle shape for optimal plasmon peak position; optical sensing and imaging strategies for intracellular nanoparticle detection; and addressing practical challenges in cellular applications of nanoprobes, including the use of targeting agents and control of nanoparticle aggregation.
authors.affiliation: Fitzpatrick Institute of Photonics, Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.
authors.forename: Hsiangkuo
authors.initials: H
authors.lastname: Yuan
authors.forename: Janna K
authors.initials: JK
authors.lastname: Register
authors.forename: Hsin-Neng
authors.initials: HN
authors.lastname: Wang
authors.forename: Andrew M
authors.initials: AM
authors.lastname: Fales
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Tuan
authors.initials: T
authors.lastname: Vo-Dinh
mesh_terms: D000818:Animals; D002477:Cells / Q000737:chemistry*; D006801:Humans; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods; D053768:Metal Nanoparticles / Q000737:chemistry*; D015335:Molecular Probes / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods
chemical_list: D015335:Molecular Probes