title: Simultaneous surface display and secretion of proteins from mammalian cells facilitate efficient in vitro selection and maturation of antibodies.
abstract: A mammalian expression system has been developed that permits simultaneous cell surface display and secretion of the same protein through alternate splicing of pre-mRNA. This enables a flexible system for in vitro protein evolution in mammalian cells where the displayed protein phenotype remains linked to genotype, but with the advantage of soluble protein also being produced without the requirement for any further recloning to allow a wide range of assays, including biophysical and cell-based functional assays, to be used during the selection process. This system has been used for the simultaneous surface presentation and secretion of IgG during antibody discovery and maturation. Presentation and secretion of monomeric Fab can also be achieved to minimize avidity effects. Manipulation of the splice donor site sequence enables control of the relative amounts of cell surface and secreted antibody. Multi-domain proteins may be presented and secreted in different formats to enable flexibility in experimental design, and secreted proteins may be produced with epitope tags to facilitate high-throughput testing. This system is particularly useful in the context of in situ mutagenesis, as in the case of in vitro somatic hypermutation.
authors.affiliation: AnaptysBio, Inc, San Diego, California 92121, USA. rhorlick@anaptysbio.com
authors.forename: Robert A
authors.initials: RA
authors.lastname: Horlick
authors.forename: John L
authors.initials: JL
authors.lastname: Macomber
authors.forename: Peter M
authors.initials: PM
authors.lastname: Bowers
authors.forename: Tamlyn Y
authors.initials: TY
authors.lastname: Neben
authors.forename: Geoffery L
authors.initials: GL
authors.lastname: Tomlinson
authors.forename: Irina P
authors.initials: IP
authors.lastname: Krapf
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Dalton
authors.forename: Petra
authors.initials: P
authors.lastname: Verdino
authors.forename: David J
authors.initials: DJ
authors.lastname: King
mesh_terms: D017398:Alternative Splicing; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000235:genetics; D000915:Antibody Affinity / Q000235:genetics*; D019020:Directed Molecular Evolution; D015870:Gene Expression; D057809:HEK293 Cells; D006801:Humans; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000235:genetics; D012322:RNA Precursors / Q000096:biosynthesis / Q000235:genetics
chemical_list: D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D012322:RNA Precursors
keywords: Affinity Maturation; Alternative Splicing; Antibodies; Antibody Engineering; Mutagenesis; Protein Display; Protein Evolution; Somatic Hypermutation