title: Fine mapping of BH1, a gene controlling lemma and palea development in rice.
abstract: A new rice floral organ mutant bh1 , had a negative effect on grain yield. BH1 was fine mapped to 87.5 kb on chr2. A 55 kb chromosome segment was deleted in bh1. The cereal spikelet is enclosed by the lemma and palea. The lemma and palea of the floral mutant designated bh1, a selection from a T-DNA library generated from the rice cultivar Asominori, takes on an abnormal curve-shaped appearance only late in floral development, finally forming a beak-shaped hull. The mutation had a negative effect on thousand grain weight, seed set rate and germination rate. Genetic analysis indicated that the mutant phenotype was determined by a single recessive gene. Through map-based approach, BH1 gene was finally located to a ~87.5-kbp region on the long arm of chromosome 2. An analysis of the gene content of this region indicated that the mutation involves the loss of a 55-kbp stretch, harboring four open reading frames. Transcription profiling based on qRT-PCR revealed that the genes OsMADS1, OsMADS14, OsMADS15, OsMADS18, REP1, CFO1, and DL, all of which are also involved in lemma and palea development and identity specification, were down-regulated in the bh1 mutant. BH1 is therefore an important floral organ development gene.
authors.affiliation: State Key Laboratory of Rice Biology, Key Laboratory of Rice Biology and Breeding of Ministry of Agriculture, China National Rice Research Institute, Hangzhou, 310006, China.
authors.forename: Xiangjin
authors.initials: X
authors.lastname: Wei
authors.forename: Xuanwen
authors.initials: X
authors.lastname: Zhang
authors.forename: Gaoneng
authors.initials: G
authors.lastname: Shao
authors.forename: Jiwai
authors.initials: J
authors.lastname: He
authors.forename: Guiai
authors.initials: G
authors.lastname: Jiao
authors.forename: Lihong
authors.initials: L
authors.lastname: Xie
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Sheng
authors.forename: Shaoqing
authors.initials: S
authors.lastname: Tang
authors.forename: Peisong
authors.initials: P
authors.lastname: Hu
mesh_terms: D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D004269:DNA, Bacterial / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D015536:Down-Regulation; D035264:Flowers / Q000235:genetics / Q000254:growth & development*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D018506:Gene Expression Regulation, Plant; D005808:Genes, Recessive; D018525:Germination; D009154:Mutation; D016366:Open Reading Frames; D012275:Oryza / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D012639:Seeds / Q000235:genetics / Q000254:growth & development; D017384:Sequence Deletion
chemical_list: D004269:DNA, Bacterial; D018744:DNA, Plant; D010940:Plant Proteins; C036483:T-DNA