title: Identification of the sensory nerve fiber responsible for lysophosphatidic acid-induced allodynia in mice.
abstract: Lysophosphatidic acid (LPA) has been considered one of the molecular culprits for neuropathic pain. Understanding how LPA changes the function of primary afferent fibers might be an essential step for clarifying the pathogenesis of neuropathic pain. The present study was designed to identify the primary afferent fibers (Aβ, Aδ, or C) participating in LPA-induced allodynia in ddY mice. Mechanical allodynia and thermal hyperalgesia were evaluated by the von Frey filament test and thermal paw withdrawal test, respectively. Sensory nerve fiber responsiveness was measured using a Neurometer. Daily repeated intrathecal treatment with LPA led to a decrease in the mechanical, but not thermal nociceptive threshold, and a reduction in the threshold for paw withdrawal induced by 2000-Hz (Aβ fiber) and 250-Hz (Aδ fiber), but not 5-Hz (C fiber) sine-wave electrical stimulation. When the transient receptor potential cation channel subfamily V member 1 (TRPV1) receptor agonist resiniferatoxin (RTX) was administered subcutaneously before the start of LPA treatment, LPA-induced mechanical allodynia and Aβ and Aδ fiber hypersensitivity demonstrated by neurometry were not affected, indicating that TRPV1-expressing nerve fibers (possibly C fibers) might not be essential for LPA-induced allodynia. LPA-induced allodynia was reversed by treatment with RTX at 7 days after the start of LPA treatment. Expression of TRPV1 on myelinated nerve fibers after repeated intrathecal LPA treatment was observed in the dorsal root ganglion. These results suggest that sensitization of Aβ and Aδ fibers, but not C fibers, contributes to the development of intrathecally administered LPA-induced mechanical allodynia. Moreover, increased or newly expressed TRPV1 receptors in Aβ and Aδ fibers are considered to be involved in the maintenance of LPA-induced allodynia.
authors.affiliation: Laboratory of CNS Pharmacology, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya 467-8603, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Ohsawa
authors.forename: Y
authors.initials: Y
authors.lastname: Miyabe
authors.forename: H
authors.initials: H
authors.lastname: Katsu
authors.forename: S
authors.initials: S
authors.lastname: Yamamoto
authors.forename: H
authors.initials: H
authors.lastname: Ono
mesh_terms: D000818:Animals; D004558:Electric Stimulation / Q000379:methods; D006358:Hot Temperature / Q000009:adverse effects; D006930:Hyperalgesia / Q000139:chemically induced / Q000378:metabolism*; D008246:Lysophospholipids / Q000633:toxicity*; D051379:Mice; D009412:Nerve Fibers / Q000187:drug effects / Q000378:metabolism; D036421:Nerve Fibers, Unmyelinated / Q000187:drug effects / Q000378:metabolism*; D010147:Pain Measurement / Q000187:drug effects / Q000379:methods*; D010812:Physical Stimulation / Q000009:adverse effects; D011984:Sensory Receptor Cells / Q000187:drug effects / Q000378:metabolism*; D050916:TRPV Cation Channels / Q000096:biosynthesis*
chemical_list: D008246:Lysophospholipids; D050916:TRPV Cation Channels; C493921:TRPV1 protein, mouse; C032881:lysophosphatidic acid
keywords: DRG; LPA; Neurometer test; PBS; RTX; TRPV1; TRPV1 immunoreactivity; TRPV1-ir; dorsal root ganglion; lysophosphatidic acid; lysophosphatidic acid (LPA); mechanical allodynia; myelinated A fiber; phosphate-buffered saline; resiniferatoxin; transient receptor potential cation channel subfamily V member 1