title: Use of a large Stokes-shift fluorophore to increase the multiplexing capacity of a point-of-care DNA diagnostic device.
abstract: The intense demand for fluorescence-based point of care (POC) DNA diagnostics is driving developments to reduce the size of instrumentation, imposing limitations on the optical hardware that can be included. Here we describe a combination of instrumentation and fluorogenic probes to detect three fluorophores using two excitation and two detection channels.
authors.affiliation: Chemistry, University of Southampton, Southampton, Hampshire SO17 1BJ, UK.
authors.forename: James A
authors.initials: JA
authors.lastname: Richardson
authors.forename: Trevor
authors.initials: T
authors.lastname: Morgan
authors.forename: Michael
authors.initials: M
authors.lastname: Andreou
authors.forename: Tom
authors.initials: T
authors.lastname: Brown
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry* / Q000235:genetics; D005456:Fluorescent Dyes / Q000737:chemistry*; D009691:Nucleic Acid Denaturation; D019095:Point-of-Care Systems; D013050:Spectrometry, Fluorescence / Q000379:methods*; D044366:Transition Temperature
chemical_list: D005456:Fluorescent Dyes; D004247:DNA