title: Transplantation of human mesenchymal stem cells loaded on collagen scaffolds for the treatment of traumatic brain injury in rats.
abstract: Studies have suggested that mesenchymal stem cells (MSCs) have therapeutic effects following traumatic brain injury (TBI). However, cell distribution and survival rate are two major barriers to their success as therapeutic treatment. The improvement of cell therapy using collagen delivery matrices had been reported. However, we know very little about the mechanisms. We labeled human bone marrow-derived mesenchymal stem cells (hMSCs) with a positron emission tomography (PET) tracer, 18F-fluoro-2-deoxy-D-glucose (FDG). hMSCs were transplanted with or without collagen scaffolds into rats with experimental TBI and the whole-body nuclear images were compared. Collagen scaffolds increased the retention of hBMSC in the lesion site and limited its distribution at the transplanted region. Significantly more hMSCs were detected in the brain when transplanted with collagen scaffolds. The results showed collagen scaffolds also efficiently improved cell survival and neurite outgrowth in vivo, resulting in better neural functional recovery. In addition, brain metabolism also improved in the collagen scaffold implanted group, as evaluated by PET. We speculated that collagen scaffolds would improve early engraftment and support the survival of grafted cells post-transplantation.
authors.affiliation: Department of Neurosurgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Guan
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Zhu
authors.forename: Robert Chunhua
authors.initials: RC
authors.lastname: Zhao
authors.forename: Zhifeng
authors.initials: Z
authors.lastname: Xiao
authors.forename: Chenxi
authors.initials: C
authors.lastname: Wu
authors.forename: Qin
authors.initials: Q
authors.lastname: Han
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.forename: Weimin
authors.initials: W
authors.lastname: Tong
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Han
authors.forename: Jun
authors.initials: J
authors.lastname: Gao
authors.forename: Ming
authors.initials: M
authors.lastname: Feng
authors.forename: Xinjie
authors.initials: X
authors.lastname: Bao
authors.forename: Jianwu
authors.initials: J
authors.lastname: Dai
authors.forename: Renzhi
authors.initials: R
authors.lastname: Wang
mesh_terms: D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology; D001930:Brain Injuries / Q000000981:diagnostic imaging / Q000503:physiopathology / Q000628:therapy*; D002454:Cell Differentiation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003094:Collagen / Q000494:pharmacology* / Q000648:ultrastructure; D019788:Fluorodeoxyglucose F18; D005782:Gels; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000000981:diagnostic imaging / Q000187:drug effects; D049268:Positron-Emission Tomography; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling; D014018:Tissue Distribution / Q000187:drug effects; D054457:Tissue Scaffolds / Q000737:chemistry*; D016896:Treatment Outcome; D051598:Whole Body Imaging
chemical_list: D005782:Gels; D019788:Fluorodeoxyglucose F18; D003094:Collagen; D005947:Glucose