title: Imidapril inhibits right ventricular remodeling induced by low ambient temperature in broiler chickens.
abstract: This study explored the effect of imidapril on the right ventricular remodeling induced by low ambient temperature in broiler chickens. Twenty-four broiler chickens were randomly divided into 3 groups (n = 8), including the control group, low temperature group, and imidapril group. Chickens in the control group were raised at normal temperature, whereas chickens in the low temperature group and imidapril group were exposed to low ambient temperature (12 to 18°C) from 14 d of age until 45 d of age. At the same time, chickens in the imidapril group were gavaged with imidapril at 3 mg/kg once daily for 30 d. The thickness of the right ventricular wall was observed with echocardiography. The BW and wet lung weight as well as weight of right and left ventricles and ventricular septum were measured. Both wet lung weight index and right ventricular hypertrophy index were calculated. Pulmonary arterial systolic pressure was assessed according to echocardiography. The expression of ACE and ACE2 mRNA in the right ventricular myocardial tissue was quantified by real-time PCR. Proliferating cell nuclear antigen-positive cells were detected by immunohistostaining. The concentration of angiotensin (Ang) II and Ang (1-7) in the right ventricular myocardial tissue was measured with ELISA. The results showed that right ventricular hypertrophy index, wet lung weight index, pulmonary arterial systolic pressure, expression of ACE mRNA in the right ventricular tissue, Ang II concentration, and the thickness of the right ventricular wall in the low temperature group increased significantly compared with those in the control group and imidapril group. The ACE2 mRNA expression increased 36%, whereas Ang (1-7) concentration decreased significantly in the low temperature group compared with that in the control group and imidapril group. In conclusion, imidapril inhibits right ventricular remodeling induced by low ambient temperature in broiler chickens.
authors.affiliation: Department of Pharmacy, College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China.
authors.forename: Xue-Qin
authors.initials: XQ
authors.lastname: Hao
authors.forename: Shou-Yan
authors.initials: SY
authors.lastname: Zhang
authors.forename: Xiang-Chao
authors.initials: XC
authors.lastname: Cheng
authors.forename: Meng
authors.initials: M
authors.lastname: Li
authors.forename: Tong-Wen
authors.initials: TW
authors.lastname: Sun
authors.forename: Ji-Liang
authors.initials: JL
authors.lastname: Zhang
authors.forename: Wen
authors.initials: W
authors.lastname: Guo
authors.forename: Li
authors.initials: L
authors.lastname: Li
mesh_terms: D000803:Angiotensin I / Q000235:genetics / Q000378:metabolism; D000804:Angiotensin II / Q000235:genetics / Q000378:metabolism; D000085962:Angiotensin-Converting Enzyme 2; D000822:Animal Husbandry; D000818:Animals; D002645:Chickens; D003080:Cold Temperature; D005786:Gene Expression Regulation; D006352:Heart Ventricles / Q000473:pathology*; D006799:Housing, Animal; D048289:Imidazolidines / Q000494:pharmacology*; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism; D007703:Peptidyl-Dipeptidase A / Q000235:genetics / Q000378:metabolism; D020257:Ventricular Remodeling / Q000187:drug effects*
chemical_list: D048289:Imidazolidines; D010446:Peptide Fragments; D000804:Angiotensin II; D000803:Angiotensin I; C065166:imidapril; D007703:Peptidyl-Dipeptidase A; D000085962:Angiotensin-Converting Enzyme 2; C118790:angiotensin I (1-7)