title: Identification of 11 new exoproteins in Corynebacterium pseudotuberculosis by comparative analysis of the exoproteome.
abstract: This study involves the comparison between the exoproteomes of two different strains of Corynebacterium pseudotuberculosis, the etiologic agent of caseous lymphadenitis in small ruminants. In a previous study, based on a gel-free system (TPP-LC/MS(E)), 70 exoproteins for the strain 1002 and 67 for the strain C231, totaling 93 different extracellular proteins for C. pseudotuberculosis, were identified. In the present work, we have used 2D gel electrophoresis to resolve the extracellular proteins of both strains, which were then digested with trypsin, analyzed by MALDI-TOF/TOF and identified with the software MASCOT(®). A total of 45 extracellular proteins of C. pseudotuberculosis were identified by this approach. The comparative analysis between the strains 1002 and C231 identified 13 and 3 strain-specific proteins, respectively, 11 of which are novel. These newly identified proteins may play an important role in the physiology and virulence of C. pseudotuberculosis.
authors.affiliation: Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antonio Carlos, 6627 - Pampulha, CP 486, CEP 31.270-901 Belo Horizonte-MG, Brazil.
authors.forename: Wanderson M
authors.initials: WM
authors.lastname: Silva
authors.forename: Núbia
authors.initials: N
authors.lastname: Seyffert
authors.forename: Agenor V
authors.initials: AV
authors.lastname: Santos
authors.forename: Thiago L P
authors.initials: TL
authors.lastname: Castro
authors.forename: Luis G C
authors.initials: LG
authors.lastname: Pacheco
authors.forename: Anderson R
authors.initials: AR
authors.lastname: Santos
authors.forename: Alessandra
authors.initials: A
authors.lastname: Ciprandi
authors.forename: Fernanda A
authors.initials: FA
authors.lastname: Dorella
authors.forename: Hélida M
authors.initials: HM
authors.lastname: Andrade
authors.forename: Debmalya
authors.initials: D
authors.lastname: Barh
authors.forename: Adriano M C
authors.initials: AM
authors.lastname: Pimenta
authors.forename: Artur
authors.initials: A
authors.lastname: Silva
authors.forename: Anderson
authors.initials: A
authors.lastname: Miyoshi
authors.forename: Vasco
authors.initials: V
authors.lastname: Azevedo
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000032:analysis* / Q000737:chemistry; D003354:Corynebacterium Infections / Q000382:microbiology / Q000662:veterinary; D016925:Corynebacterium pseudotuberculosis / Q000737:chemistry* / Q000145:classification*; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D008199:Lymphadenitis / Q000382:microbiology / Q000662:veterinary; D020543:Proteome; D040901:Proteomics / Q000379:methods; D013045:Species Specificity; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D001426:Bacterial Proteins; D020543:Proteome
keywords: 2-DE; CLA; Caseous lymphadenitis; Corynebacterium pseudotuberculosis; Extracellular proteins; HSP; LC-MS; MALDI-TOF; MS; PLD; PSE; Proteomics; RT; TPP; Two-dimensional electrophoresis (2-DE); caseous lymphadenitis; heat shock protein; liquid chromatography – mass spectrometry; mass spectrometer; matrix-assisted laser desorption/ionization – time-of-flight; phospholipase D; potentially surface exposed; room temperature; three-Phase partitioning; two-dimensional electrophoresis