title: Induction of cytopathic effect and cytokines in coxsackievirus B3-infected murine astrocytes.
abstract: BACKGROUND
  Coxsackievirus commonly infects children and occasionally causes severe meningitis and/or encephalitis in the newborn. The underlying mechanism(s) behind the central nervous system pathology is poorly defined.
  
  
  METHODS
  It is hypothesized that astrocytes may be involved in inflammatory response induced by CVB3 infection. Here we discuss this hypothesis in the context of CVB3 infection and associated inflammatory response in primary mouse astrocytes.
  
  
  RESULTS
  The results showed that coxsackievirus receptor (CAR) was distributed homogeneously on the astrocytes, and that CVB3 could infect and replicate in astrocytes, with release of infectious virus particles. CVB3 induced cytopathic effect and production of proinflammatory cytokines IL-1β, TNF-α, IL-6, and chemokine CXCL10 from astrocytes.
  
  
  CONCLUSIONS
  These data suggest that direct astrocyte damage and cytokines induction could be a mechanism of virus-induced meningitis and/or encephalitis.
authors.affiliation: Key Laboratory of Infectious Diseases and Molecular Immunopathology of Guangdong Province, Department of Microbiology & Immunology, Shantou University Medical College, 22 Xinling Road, Shantou 515041, P.R. China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zeng
authors.forename: Gefei
authors.initials: G
authors.lastname: Wang
authors.forename: Weizhong
authors.initials: W
authors.lastname: Li
authors.forename: Dangui
authors.initials: D
authors.lastname: Zhang
authors.forename: Xiaoxuan
authors.initials: X
authors.lastname: Chen
authors.forename: Gang
authors.initials: G
authors.lastname: Xin
authors.forename: Zhiwu
authors.initials: Z
authors.lastname: Jiang
authors.forename: Kangsheng
authors.initials: K
authors.lastname: Li
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000502:physiology / Q000821:virology*; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism*; D003588:Cytopathogenic Effect, Viral; D029822:Enterovirus B, Human / Q000276:immunology* / Q000472:pathogenicity*; D051379:Mice; D008807:Mice, Inbred BALB C; D014779:Virus Replication
chemical_list: D016207:Cytokines