title: The Golgi-Cox method.
abstract: One of the best neurohistologic methods to reveal the cytoarchitecture of the brain and detailed morphology of neurons with unsurpassed clarity has been the Golgi staining. It is based on the principle of metallic impregnation of neurons, allowing visualization in their entirety including cell soma, axons, dendrites, and spines. In this chapter, we describe the Golgi-Cox protocol standardized in our laboratory that can be used to study experimental effects of different genetic manipulations on spatial distribution of neurons, dendrite density, spine number and morphology to elucidate gene functions during development and in adult brain.
authors.affiliation: Department of Chemical Biology, Susan Lehman Cullman Laboratory for Cancer Research, Ernest Mario School of Pharmacy, Rutgers University, Piscataway, NJ, USA.
authors.forename: Gitanjali
authors.initials: G
authors.lastname: Das
authors.forename: Kenneth
authors.initials: K
authors.lastname: Reuhl
authors.forename: Renping
authors.initials: R
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology; D003116:Color; D003890:Desiccation; D004210:Dissection; D009474:Neurons / Q000166:cytology; D013194:Staining and Labeling / Q000379:methods*