title: Simultaneous determination of nucleosides and their bases in Cordyceps sinensis and its substitutes by matrix solid-phase dispersion extraction and HPLC.
abstract: Nine nucleosides and nucleobases, including uracil, adenine, thymine, uridine, adenosine, thymidine, cytidine, guanosine, and cordycepin in natural Cordyceps sinensis, cultured Cordyceps mycelia, and Cordyceps fruiting bodies were extracted by matrix solid-phase dispersion (MSPD) and determined by HPLC. The experimental conditions for the MSPD extraction were optimized. Florisil was used as dispersant, petroleum ether as washing solvent, and methanol as elution solvent. The Florisil-to-sample ratio was selected to be 4:1 and no additional clean-up sorbent was needed. The calibration curves had good linear relationships (r > 0.9997). The LOD and LOQ were in the range of 12～79 and 41～265 ng/mL, respectively. The intra- and interday precision were lower than 8.3%. The recoveries were between 61.5 and 93.2%. The present method consumed less sample compared with ultrasonic extraction and heating reflux extraction (HRE). The extraction yields obtained by using the present method are much higher than those obtained by UE and comparable to those obtained by HRE.
authors.affiliation: College of Chemistry, Jilin University, Changchun, PR China.
authors.forename: Zhibing
authors.initials: Z
authors.lastname: Wang
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Du
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Ji
authors.forename: Aimin
authors.initials: A
authors.lastname: Yu
authors.forename: Hanqi
authors.initials: H
authors.lastname: Zhang
authors.forename: Fangping
authors.initials: F
authors.lastname: Qiu
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D032661:Cordyceps / Q000737:chemistry*; D009705:Nucleosides / Q000032:analysis* / Q000302:isolation & purification; D009711:Nucleotides / Q000032:analysis* / Q000302:isolation & purification; D010936:Plant Extracts / Q000032:analysis* / Q000302:isolation & purification; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D009705:Nucleosides; D009711:Nucleotides; D010936:Plant Extracts
keywords: Cordyceps sinensis; HPLC; Matrix solid-phase dispersion; Nucleo-bases; Nucleosides