title: The effects of endothelial lipase gene (LIPG) variants on inflammation marker levels and atherosclerosis development.
abstract: Atherosclerosis is a major pathological process related with several important adverse vascular events including coronary artery disease, stroke, and peripheral arterial disease. Endothelial lipase is an enzyme the activity of which affects all of lipoproteins, whereas HDL is the main substrate. The purpose of our study was to investigate the effects of endothelial lipase gene polymorphism and inflammation markers (CRP, IL-1β, IL-6, IL-8 and TNF-α) in the atherosclerosis. 104 patients with atherosclerosis and 76 healthy individuals were included in the study. LIPG -584C/T polymorphism gene polymorphisms were assessed with PCR-RFLP method. The serum CRP levels were measured by turbidimetric method using a biochemistry autoanalyzer, whereas serum IL-1β, IL-6, IL-8, TNF-α levels were determined by enzyme-linked immunosorbent assay. In this study, we found that the frequencies of TC genotype are more prevalent in patients than controls. We found a statistically significant difference of IL-6 levels between patient and control group. Our findings suggest that T allele might play a potential role in the susceptibility to atherogenesis in the Turkish population.
authors.affiliation: Yeditepe University Hospital, Istanbul, Turkey.
authors.forename: Altay Burak
authors.initials: AB
authors.lastname: Dalan
authors.forename: Bahar
authors.initials: B
authors.lastname: Toptaş
authors.forename: Zehra
authors.initials: Z
authors.lastname: Buğra
authors.forename: Nihat
authors.initials: N
authors.lastname: Polat
authors.forename: Hülya
authors.initials: H
authors.lastname: Yılmaz-Aydoğan
authors.forename: Arif
authors.initials: A
authors.lastname: Çimen
authors.forename: Turgay
authors.initials: T
authors.lastname: Isbir
mesh_terms: D050197:Atherosclerosis / Q000201:enzymology* / Q000503:physiopathology; D016022:Case-Control Studies; D016207:Cytokines / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D014644:Genetic Variation; D006801:Humans; D008049:Lipase / Q000235:genetics* / Q000378:metabolism*; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D018709:Statistics, Nonparametric; D014421:Turkey
chemical_list: D016207:Cytokines; C410382:LIPG protein, human; D008049:Lipase