title: Functional analysis of PsG6PDH, a cytosolic glucose-6-phosphate dehydrogenase gene from Populus suaveolens, and its contribution to cold tolerance improvement in tobacco plants.
abstract: A 1,697-bp cDNA sequence, designated as PsG6PDH, was amplified from Populus suaveolens. Multiple sequence alignment and phylogenetic analysis indicated that PsG6PDH encodes a cytosolic G6PDH isoform, with Southern blot analysis demonstrating that the gene is single or low copy in Populus. Transgenic tobacco plants over-expressing PsG6PDH exhibited enhanced cold tolerance. In both transgenic and wild-type (WT) tobacco plants, cold stress increased leaf malondialdehyde (MDA) content, electrolyte leakage (EL), and peroxide (POD) and superoxide dismutase (SOD) activities; relative to WT, however, transgenic lines had lower MDA content and EL and higher SOD and POD activities. In addition, PsG6PDH activated the expression of stress-related genes, including NtERD10b, NtERD10c, and NtSOD, in tobacco plants. Our results provide evidence regarding PsG6PDH regulatory function in plants during low temperature stress.
authors.affiliation: College of Forestry, South China Agricultural University, Guangzhou, 510642, China. yzhlin@scau.edu.cn
authors.forename: Yuanzhen
authors.initials: Y
authors.lastname: Lin
authors.forename: Shanzhi
authors.initials: S
authors.lastname: Lin
authors.forename: Hai
authors.initials: H
authors.lastname: Guo
authors.forename: Zhiyi
authors.initials: Z
authors.lastname: Zhang
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Chen
mesh_terms: D003080:Cold Temperature; D015870:Gene Expression; D020869:Gene Expression Profiling; D005954:Glucosephosphate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D008315:Malondialdehyde / Q000032:analysis; D010545:Peroxides / Q000032:analysis; D010802:Phylogeny; D018515:Plant Leaves / Q000737:chemistry; D030821:Plants, Genetically Modified; D032107:Populus / Q000201:enzymology* / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D013312:Stress, Physiological; D013482:Superoxide Dismutase / Q000032:analysis; D014026:Nicotiana / Q000201:enzymology* / Q000235:genetics / Q000528:radiation effects*
chemical_list: D010545:Peroxides; D011994:Recombinant Proteins; D008315:Malondialdehyde; D005954:Glucosephosphate Dehydrogenase; D013482:Superoxide Dismutase