title: [The study of serine/threonine kinase signaling pathway-mediated inhibition of proliferation and invasion of oral squamous cell carcinoma transfected with p53 gene].
abstract: OBJECTIVE
  To evaluate the molecular mechanism of proliferation and invasion inhibition in oral squamous cell carcinoma transfected with recombinant adenovirus-p53 (Ad-p53).
  
  
  METHODS
  Tca8113 cell lines were transfected with Ad-p53. Then the effect of p53 overexpression on cancer cells proliferation and invasion was observed. The expression of mitogen-activated protein kinase (MAPK), serine/threonine kinase (AKT) signaling pathway related proteins, cell cycle and apoptosis related proteins Cyclin D1, P21 and Bcl-2 were detected by Western blotting analysis.
  
  
  RESULTS
  After transfected with Ad-p53, the proliferation and invasion of Tca8113 cells were significantly inhibited (P < 0.01) and the apoptosis of Tca8113 cells significantly increased (P < 0.001). The results of Western blotting demonstrated that the protein expression of P53 and P21 significantly increased, Cyclin D1 and Bcl-2 protein expression and phosphorylation of AKT protein significantly decreased (P < 0.01).
  
  
  CONCLUSIONS
  The AKT signaling pathway may be a key molecular mechanism for proliferation and invasion inhibition of oral squamous cell carcinoma caused by p53. The protein of Cyclin D1, P21 and Bcl-2 may be the downstream targets of AKT signaling pathway. This may provide a new evidence for AKT pathway and downstream targets as a promising therapeutic target for malignant tumors.
authors.affiliation: Dept. of Stomatology, The First People's Hospital of Zhenjiang City, Zhenjiang 212002, China.
authors.forename: Jibin
authors.initials: J
authors.lastname: Gao
authors.forename: Xingping
authors.initials: X
authors.lastname: Li
authors.forename: Jidang
authors.initials: J
authors.lastname: Jiang
mesh_terms: D017209:Apoptosis; D002294:Carcinoma, Squamous Cell; D002453:Cell Cycle; D049109:Cell Proliferation; D019938:Cyclin D1; D016158:Genes, p53; D006801:Humans; D020928:Mitogen-Activated Protein Kinases; D009062:Mouth Neoplasms; D017346:Protein Serine-Threonine Kinases; D012694:Serine; D015398:Signal Transduction; D014162:Transfection
chemical_list: D019938:Cyclin D1; D012694:Serine; D017346:Protein Serine-Threonine Kinases; D020928:Mitogen-Activated Protein Kinases