title: Morphology and growth of mammalian cells in a liquid/liquid culture system supported with oxygenated perfluorodecalin.
abstract: Adherent A431, BHK-21, and C2C12 cells were cultured on a flexible interface formed between two immiscible liquid phases: (i) hydrophobic perfluorodecalin (PFD) and (ii) aqueous culture medium (DMEM). BHK-21 cells formed multicellular aggregates characterized by irregular shapes. A431, as well as C2C12 cells, grew as tight multicellular sheets of 3-D cells. Enhanced mass transfer and facilitated access of the cells to the O2 dissolved in PFD/DMEM by approx. 250 % and thereby increased the density of BHK-21 cells. Thus the liquid/liquid system is a simple, ready-to-use, and fully scalable (independent of vessel shapes); consequently it is a method for 3-D cultures of adherent animal cells in which the growth of anchorage-dependent cells is not limited by confluence effect.
authors.affiliation: Biotechnology and Bioprocess Engineering Division, Faculty of Chemical and Process Engineering, Warsaw University of Technology, Waryńskiego 1, 00-645, Warsaw, Poland. pilarek@ichip.pw.edu.pl
authors.forename: Maciej
authors.initials: M
authors.lastname: Pilarek
authors.forename: Iwona
authors.initials: I
authors.lastname: Grabowska
authors.forename: Maria A
authors.initials: MA
authors.lastname: Ciemerych
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Dąbkowska
authors.forename: Krzysztof W
authors.initials: KW
authors.lastname: Szewczyk
mesh_terms: D000818:Animals; D019149:Bioreactors; D018929:Cell Culture Techniques; D003470:Culture Media / Q000737:chemistry*; D005057:Eukaryotic Cells / Q000502:physiology*; D005466:Fluorocarbons / Q000378:metabolism*; D006801:Humans; D008322:Mammals; D010100:Oxygen / Q000378:metabolism
chemical_list: D003470:Culture Media; D005466:Fluorocarbons; C008805:perfluorodecalin; D010100:Oxygen