title: Comparative analysis of monocyte subsets in the pig.
abstract: Human and mouse monocyte can be divided into two different subpopulations based on surface marker expression: CD14/16 and Ly6C/CX3CR1, respectively. Monocyte subpopulations in the pig were identified based on reciprocal expression of CD14 and the scavenger receptor CD163. The two populations, CD14(hi)-CD163(low) and CD14(low)-CD163(hi), show approximately equal abundance in the steady-state. Culture of pig PBMCs in CSF1 indicates that the two populations are a maturation series controlled by this growth factor. Gene expression in pig monocyte subpopulations was profiled using the newly developed and annotated pig whole genome snowball microarray. Previous studies have suggested a functional equivalence between human and mouse subsets, but certain genes such as CD36, CLEC4E, or TREM-1 showed human-specific expression. The same genes were expressed selectively in pig monocyte subsets. However, the profiles suggest that the pig CD14(low)-CD163(high) cells are actually equivalent to intermediate human monocytes, and there is no CD14(-) CD16(+) "nonclassical" population. The results are discussed in terms of the relevance of the pig as a model for understanding human monocyte function.
authors.affiliation: The Roslin Institute and The Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian EH25 9RG, United Kingdom.
authors.forename: Lynsey
authors.initials: L
authors.lastname: Fairbairn
authors.forename: Ronan
authors.initials: R
authors.lastname: Kapetanovic
authors.forename: Dario
authors.initials: D
authors.lastname: Beraldi
authors.forename: David P
authors.initials: DP
authors.lastname: Sester
authors.forename: Chris K
authors.initials: CK
authors.lastname: Tuggle
authors.forename: Alan L
authors.initials: AL
authors.lastname: Archibald
authors.forename: David A
authors.initials: DA
authors.lastname: Hume
mesh_terms: D000818:Animals; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D009000:Monocytes / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D034421:Sus scrofa / Q000276:immunology*; D013552:Swine; D059467:Transcriptome