title: Ectopic hair cell-like cell induction by Math1 mainly involves direct transdifferentiation in neonatal mammalian cochlea.
abstract: Math1, also known as Atoh1, is a basic helix-loop-helix transcription factor that plays a key role in hair cells (HCs) development. Previous studies have reported that Math1 gene transfer could induce the production of ectopic hair cell-like cells both in vitro and in vivo. Here, we focused on the mechanism of ectopic hair cell-like cellular differentiation from cells in the lateral epithelial ridge (LER) of cochlea with a human adenovirus serotype 5 (Ad5) vector encoding both Math1 and the reporter gene EGFP. Within the Ad5-EGFP-Math1 infection, hair-cell like cells could be detected in the LER. 5'-Bromo-2' deoxyuridine (BrdU) incorporation test results at different time points suggested that LER cells possessed high potential to proliferation, but they could not transdifferentiate into hair cells spontaneously. Almost all of Math1 induced hair cell-like cells were BrdU negative when BrdU incorporation occurred after Math1 expression. In conclusion, Math1 induced hair cell-like cells from LER cells mainly underwent direct trans-differentiation instead of mitosis of LER cells or newly hair cell-like cells.
authors.affiliation: Eye & ENT Hospital of Fudan University, Department of Otolaryngology-Head and Neck Surgery, 83 Fenyang Road, Shanghai 200031, China.
authors.forename: Juanmei
authors.initials: J
authors.lastname: Yang
authors.forename: Ning
authors.initials: N
authors.lastname: Cong
authors.forename: Zhao
authors.initials: Z
authors.lastname: Han
authors.forename: Yibo
authors.initials: Y
authors.lastname: Huang
authors.forename: Fanglu
authors.initials: F
authors.lastname: Chi
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000378:metabolism*; D002452:Cell Count; D054338:Cell Transdifferentiation / Q000502:physiology*; D003051:Cochlea / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D006198:Hair Cells, Auditory / Q000166:cytology / Q000378:metabolism*; D051381:Rats
chemical_list: D051792:Basic Helix-Loop-Helix Proteins
keywords: BrdU incorporation test; Direct transdifferentiation; Lateral epithelial ridge (LER); Math1/Atoh1