title: Cloning and application of recombinant dengue virus prM-M protein for serodiagnosis of dengue virus infection.
abstract: We studied the use of the precursor to the M structural protein (prM) found only on the surface of mature dengue virus as a target protein to detect dengue virus infection. Recombinant D2-16681 prM-M protein was constructed and tested for immunogenicity with dengue and Japanese encephalitis patient sera by Western blot analysis and indirect ELISA. The sensitivity and specificity of indirect ELISA were 48.1 and 85.5%, respectively, and Western blot assay were 23.1 and 98.7%, respectively, for detection of dengue virus. Although the sensitivity of the indirect ELISA is low, the indirect ELISA using recombinant D2-16681 prM-M proteins as antigen may be used for early detection of dengue virus infection.
authors.affiliation: Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Sineewanlaya
authors.initials: S
authors.lastname: Wichit
authors.forename: Hatairat
authors.initials: H
authors.lastname: Yongpradoem
authors.forename: Pornapat
authors.initials: P
authors.lastname: Surasombatpattana
authors.forename: Pornsawan
authors.initials: P
authors.lastname: Leaungwuttiwong
authors.forename: Thareerat
authors.initials: T
authors.lastname: Kalambaheti
authors.forename: Wipawee
authors.initials: W
authors.lastname: Jampangern
authors.forename: Akanitt
authors.initials: A
authors.lastname: Jittmittraphap
mesh_terms: D003001:Cloning, Molecular; D003715:Dengue / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D003716:Dengue Virus / Q000235:genetics* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D011994:Recombinant Proteins / Q000235:genetics; D012698:Serologic Tests; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology
chemical_list: D011994:Recombinant Proteins; D014764:Viral Proteins