title: Changes in the mobile phase composition on a stepwise counter-current chromatography elution for the isolation of flavonoids from Siparuna glycycarpa.
abstract: This paper describes the isolation of flavonoids and other aromatic compounds from an ethyl acetate extract of leaves of Siparuna glycycarpa using stepwise elution counter-current chromatography (CCC). The elution profile yielded the following compounds: diglycosylated flavonoids, quercetin 3-O-rutinoside and quercetin 7-O-rutinoside, followed by monoglycosylated flavonoids, kaempferol-3-O-β-glucopyranoside, kaempferol-3-O-β-rhamnopiranoside, kaempferol-3-O-β-6''(p-coumaroyl) glucopyranoside, and quercetin-3-O-β-glucopyranoside, and then free phenolics, protocatechuic acid, and 2',6'-dihydroxy-4, 4'-dimethoxydihydrochalcone, which shows that this type of elution covers a broader range of polarity than the traditional isocratic mode. This makes it more suitable to perform separations of mixtures containing large differences in hydrophobicity. A GC analysis of a blank CCC run was performed to determine if changes in the mobile phase composition affect the chromatographic process. Results showed a gradual variation of the composition of the mobile phase emerging after the step gradient, favoring the selectivity of the solvent system.
authors.affiliation: Universidade Federal do Rio de Janeiro, Núcleo de Pesquisas de Produtos Naturais, CCS, Ilha do Fundão, Rio de Janeiro, Brazil.
authors.forename: Fernanda das Neves
authors.initials: Fd
authors.lastname: Costa
authors.forename: Ian
authors.initials: I
authors.lastname: Garrard
authors.forename: Antonio Jorge Ribeiro
authors.initials: AJ
authors.lastname: da Silva
authors.forename: Gilda Guimarães
authors.initials: GG
authors.lastname: Leitão
mesh_terms: D003377:Countercurrent Distribution / Q000295:instrumentation / Q000379:methods*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification*; D019684:Magnoliopsida / Q000737:chemistry*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification*; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D005419:Flavonoids; D010936:Plant Extracts
keywords: Counter-current chromatography; Siparuna glycycarpa; Siparunaceae; Step gradient; Stepwise elution