title: [Chemical constituents of Rabdosia japonica var. glaucocalyx and their anti-complementary activity].
abstract: To study the chemical constituents of Rabdosia japonica var. glaucocalyx and their anti-complementary activity on the basis of preliminary studies. Target isolation guided by anti-complementary activity test, compounds in the chloroform and n-butanol fractions were isolated and purified by silica gel and Sephadex LH-20 column chromatographies, and preparative HPLC. The structures were identified by various spectroscopic data including ESI-MS, 1H-NMR and 13C-NMR data. The compounds were evaluated for anti-complementary activity in vitro. Eleven compounds were isolated from the chloroform and n-butanol soluble fractions and identified as stigmasterol (1), stigmas-9 (11) -en-3-ol (2), glaucocalyxin D (3), kamebakaurin (4), maslinic acid (5), corosolic acid (6), minheryins I (7), diosmetin (8), caffeic acid ethylene ester (9), caffeic acid (10) and vitexin (11). Isoquercetrin, rutin, quercetin, 3-methylquercetin, luteolin, 7-methylluteolin, and apigenin which were isolated from the preliminary studies together with compounds 9 and 10 showed inhibition of the complement system by the classical pathway. Compounds 2, 4, 6-9 and 11 were obtained from this plant for the first time. Caffeic acid (10) showed the strongest activity in vitro with a CH50 value of 0.041 g x L(-1).
authors.affiliation: College of Pharmaceutical Science, Soochow University, Suzhou 215123, China. yaoshixs@yahoo.com.cn
authors.forename: Shi
authors.initials: S
authors.lastname: Yao
authors.forename: Nai-Yu
authors.initials: NY
authors.lastname: Xu
authors.forename: Chun-Jun
authors.initials: CJ
authors.lastname: Chu
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Dao-Feng
authors.initials: DF
authors.lastname: Chen
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002109:Caffeic Acids / Q000494:pharmacology*; D002845:Chromatography; D002851:Chromatography, High Pressure Liquid; D015941:Complement Hemolytic Activity Assay / Q000379:methods*; D051056:Complement Inactivating Agents / Q000737:chemistry / Q000494:pharmacology*; D006224:Cricetinae; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D004912:Erythrocytes / Q000187:drug effects; D004952:Esters; D005030:Ethylenes / Q000494:pharmacology; D005260:Female; D037602:Isodon / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D035261:Plant Components, Aerial / Q000737:chemistry; D010937:Plant Growth Regulators / Q000494:pharmacology; D012756:Sheep; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000975:Antioxidants; D002109:Caffeic Acids; D051056:Complement Inactivating Agents; D004365:Drugs, Chinese Herbal; D004952:Esters; D005030:Ethylenes; D010937:Plant Growth Regulators; C036216:ethylene; C040048:caffeic acid