title: Regulation of the swarming inhibitor disA in Proteus mirabilis.
abstract: The disA gene encodes a putative amino acid decarboxylase that inhibits swarming in Proteus mirabilis. 5' rapid amplification of cDNA ends (RACE) and deletion analysis were used to identify the disA promoter. The use of a disA-lacZ fusion indicated that FlhD(4)C(2), the class I flagellar master regulator, did not have a role in disA regulation. The putative product of DisA, phenethylamine, was able to inhibit disA expression, indicating that a negative regulatory feedback loop was present. Transposon mutagenesis was used to identify regulators of disA and revealed that umoB (igaA) was a negative regulator of disA. Our data demonstrate that the regulation of disA by UmoB is mediated through the Rcs phosphorelay.
authors.affiliation: Department of Microbiology and Immunology, Emory University, Atlanta, GA, USA.
authors.forename: Bree A
authors.initials: BA
authors.lastname: Szostek
authors.forename: Philip N
authors.initials: PN
authors.lastname: Rather
mesh_terms: D019677:Artificial Gene Fusion; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D004251:DNA Transposable Elements; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D017930:Genes, Reporter; D008124:Locomotion; D016254:Mutagenesis, Insertional; D010627:Phenethylamines / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011513:Proteus mirabilis / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D001616:beta-Galactosidase / Q000032:analysis / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D010627:Phenethylamines; C029261:phenethylamine; D001616:beta-Galactosidase