title: CD8⁺ T cell help is required for efficient induction of EAE in Lewis rats.
abstract: The role of CD8⁺ T cells in multiple sclerosis (MS) and experimental autoimmune encephalomyelitis (EAE) is still unclear. We describe here significantly reduced disease activity of EAE both in Lewis rats depleted of CD8⁺ T cells by monoclonal antibodies and CD8 knockout rats, which was accompanied by reduced leukocyte infiltration into the spinal cord. We detected myelin basic protein (MBP)-specific CD8⁺ T cells in peripheral lymphoid organs of CD8-depleted animals which, however, failed to differentiate into interferon-γ-producing effector cells. Our results indicate that CD8⁺ T cells interact with myelin-specific CD8⁺ T cells early in EAE enabling them to differentiate into pathogenic effector cells.
authors.affiliation: Institute for Virology and Immunobiology, University of Würzburg, Versbacher Strasse 7, 97078 Würzburg, Germany.
authors.forename: Monika
authors.initials: M
authors.lastname: Camara
authors.forename: Niklas
authors.initials: N
authors.lastname: Beyersdorf
authors.forename: Henrike J
authors.initials: HJ
authors.lastname: Fischer
authors.forename: Marco J
authors.initials: MJ
authors.lastname: Herold
authors.forename: Chi Wang
authors.initials: CW
authors.lastname: Ip
authors.forename: Jens
authors.initials: J
authors.lastname: van den Brandt
authors.forename: Klaus V
authors.initials: KV
authors.lastname: Toyka
authors.forename: Joel D
authors.initials: JD
authors.lastname: Taurog
authors.forename: Thomas
authors.initials: T
authors.lastname: Hünig
authors.forename: Thomas
authors.initials: T
authors.lastname: Herrmann
authors.forename: Holger M
authors.initials: HM
authors.lastname: Reichardt
authors.forename: Andreas
authors.initials: A
authors.lastname: Weishaupt
authors.forename: Thomas
authors.initials: T
authors.lastname: Kerkau
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology*; D004195:Disease Models, Animal; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology* / Q000517:prevention & control* / Q000628:therapy; D005260:Female; D005434:Flow Cytometry; D007114:Immunization; D007371:Interferon-gamma / Q000276:immunology; D047589:Leukocyte Reduction Procedures; D008297:Male; D009103:Multiple Sclerosis / Q000276:immunology / Q000517:prevention & control / Q000628:therapy; D004676:Myelin Basic Protein / Q000276:immunology; D051381:Rats; D011917:Rats, Inbred Lew; D011922:Rats, Mutant Strains; D013116:Spinal Cord / Q000166:cytology / Q000276:immunology
chemical_list: D004676:Myelin Basic Protein; D007371:Interferon-gamma