title: Stem cells from umbilical cord Wharton's jelly from preterm birth have neuroglial differentiation potential.
abstract: OBJECTIVE
  The aim of the study is to determine the neuroglial differentiation potential of human Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) from preterm birth when compared to term delivery.
  
  
  METHODS
  The WJ-MSCs from umbilical cords of preterm birth and term controls were isolated and induced into neural progenitors. The cells were analyzed for neuroglial markers by flow cytometry, real-time polymerase chain reaction, and immunocytochemistry.
  
  
  RESULTS
  Independent of gestational age, a subset of WJ-MSC displayed the neural progenitor cell markers Nestin and Musashi-1 and the mature neural markers microtubule-associated protein 2, glial fibrillary acidic protein, and myelin basic protein. Neuroglial induction of WJ-MSCs from term and preterm birth resulted in the enhanced transcription of Nestin and Musashi-1.
  
  
  CONCLUSIONS
  Undifferentiated WJ-MSCs from preterm birth express neuroglial markers and can be successfully induced into neural progenitors similar to term controls. Their potential use as cellular graft in neuroregenerative therapy for peripartum brain injury in preterm birth has to be tested.
authors.affiliation: 1Department of Obstetrics and Gynecology, University Hospital Bern, Switzerland.
authors.forename: Marianne
authors.initials: M
authors.lastname: Messerli
authors.forename: Anna
authors.initials: A
authors.lastname: Wagner
authors.forename: Ruth
authors.initials: R
authors.lastname: Sager
authors.forename: Martin
authors.initials: M
authors.lastname: Mueller
authors.forename: Marc
authors.initials: M
authors.lastname: Baumann
authors.forename: Daniel V
authors.initials: DV
authors.lastname: Surbek
authors.forename: Andreina
authors.initials: A
authors.lastname: Schoeberlein
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D005260:Female; D018507:Gene Expression Regulation, Developmental; D005865:Gestational Age; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000502:physiology*; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism; D004676:Myelin Basic Protein / Q000235:genetics / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D064231:Nestin / Q000235:genetics / Q000378:metabolism; D009457:Neuroglia / Q000502:physiology*; D011247:Pregnancy; D047928:Premature Birth; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic; D059631:Wharton Jelly / Q000166:cytology*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D005904:Glial Fibrillary Acidic Protein; C489788:MAP2 protein, human; C499048:MBP protein, human; C491297:MSI1 protein, human; D008869:Microtubule-Associated Proteins; D004676:Myelin Basic Protein; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin; D016601:RNA-Binding Proteins
keywords: Wharton’s jelly; mesenchymal stem cells; neuroglial differentiation; neuroregeneration; prematurity