title: CLIPPER: an add-on to the Trans-Proteomic Pipeline for the automated analysis of TAILS N-terminomics data.
abstract: Data analysis in proteomics is complex and with the extra challenges involved in the interpretation of data from N-terminomics experiments, this can be daunting.Therefore, we have devised a rational pipeline of steps to approach N-terminomics data analysis in a statistically based and valid manner. We have automated these steps in CLIPPER, an add-on to the Trans-Proteomic Pipeline(TPP). Applying CLIPPER to the analysis of N- terminomics data generated by terminal amine isotopic labeling of substrates (TAILS) enables high confidence peptide to protein assignment, protein N-terminal characterization and annotation, and for protease analysis readily allows protease substrate discovery with high confidence.
authors.affiliation: Department of Biology, Institute of Molecular Health Sciences, ETH Zurich, CH-8093 Zurich, Switzerland. ulrich.aufdemkeller@biol.ethz.ch
authors.forename: Ulrich
authors.initials: U
authors.lastname: auf dem Keller
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Overall
mesh_terms: D007553:Isotope Labeling / Q000379:methods; D010447:Peptide Hydrolases / Q000378:metabolism; D010455:Peptides / Q000032:analysis; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D059748:Proteolysis; D040901:Proteomics / Q000379:methods*; D012984:Software
chemical_list: D010455:Peptides; D011506:Proteins; D010447:Peptide Hydrolases