title: Production of 10-hydroxy-12,15(Z,Z)-octadecadienoic acid from α-linolenic acid by permeabilized cells of recombinant Escherichia coli expressing the oleate hydratase gene of Stenotrophomonas maltophilia.
abstract: Recombinant Escherichia coli, expressing the oleate hydratase gene of Stenotrophomonas maltophilia, was permeabilized by sequential treatments with 0.125 M NaCl and 2 mM EDTA. The optimal conditions for the production of 10-hydroxy-12,15(Z,Z)-octadecadienoic acid from α-linolenic acid by permeabilized cells were 35 °C and pH 7.0 with 0.1 % (v/v) Tween 40, 50 g permeabilized cells l(-1), and 17.5 g α-linolenic acid l(-1). Under these conditions, permeabilized cells produced 14.3 g 10-hydroxy-12,15(Z,Z)-octadecadienoic acid l(-1) after 18 h, with a conversion yield of 82 % (g/g) and a volumetric productivity of 0.79 g l(-1) h(-1). These values were 17 and 168 % higher than those obtained by nonpermeabilized cells, respectively. The concentration, yield, and productivity of 10-hydroxy-12,15(Z,Z)-octadecadienoic acid obtained by permeabilized cells are the highest reported thus far.
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, Republic of Korea.
authors.forename: Hye-Jin
authors.initials: HJ
authors.lastname: Oh
authors.forename: Kyung-Cheol
authors.initials: KC
authors.lastname: Shin
authors.forename: Deok-Kun
authors.initials: DK
authors.lastname: Oh
mesh_terms: D002463:Cell Membrane Permeability / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D015870:Gene Expression; D006836:Hydro-Lyases / Q000096:biosynthesis* / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D020615:Stenotrophomonas maltophilia / Q000201:enzymology* / Q000235:genetics; D013696:Temperature; D017962:alpha-Linolenic Acid / Q000378:metabolism*
chemical_list: D005231:Fatty Acids, Unsaturated; D011994:Recombinant Proteins; C495366:hydroxyoctadecadienoic acid; D017962:alpha-Linolenic Acid; D006836:Hydro-Lyases