title: Noninvasive microelectrode ion flux estimation technique (MIFE) for the study of the regulation of root membrane transport by cyclic nucleotides.
abstract: Changes in ion permeability and subsequently intracellular ion concentrations play a crucial role in intracellular and intercellular communication and, as such, confer a broad array of developmental and adaptive responses in plants. These changes are mediated by the activity of plasma-membrane based transport proteins many of which are controlled by cyclic nucleotides and/or other signaling molecules. The MIFE technique for noninvasive microelectrode ion flux measuring allows concurrent quantification of net fluxes of several ions with high spatial (μm range) and temporal (ca. 5 s) resolution, making it a powerful tool to study various aspects of downstream signaling events in plant cells. This chapter details basic protocols enabling the application of the MIFE technique to study regulation of root membrane transport in general and cyclic nucleotide mediated transport in particular.
authors.affiliation: Division of Chemical and Life Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.
authors.forename: Natalia Maria
authors.initials: NM
authors.lastname: Ordoñez
authors.forename: Lana
authors.initials: L
authors.lastname: Shabala
authors.forename: Chris
authors.initials: C
authors.lastname: Gehring
authors.forename: Sergey
authors.initials: S
authors.lastname: Shabala
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D001671:Biochemistry / Q000379:methods*; D001692:Biological Transport; D002138:Calibration; D002462:Cell Membrane / Q000378:metabolism*; D007477:Ions / Q000378:metabolism*; D008839:Microelectrodes; D009712:Nucleotides, Cyclic / Q000378:metabolism*; D018517:Plant Roots / Q000378:metabolism*; D013223:Statistics as Topic
chemical_list: D007477:Ions; D009712:Nucleotides, Cyclic