title: Isoelectric point determination of live polioviruses by capillary isoelectric focusing with whole column imaging detection.
abstract: Using a capillary isoelectric focusing-whole column imaging detection (CIEF-WCID) method, the isoelectric points (pI) of complete intact polioviruses were determined. The polioviruses that were analyzed are the commonly used viruses for the production of inactivated polio vaccines (IPV)-Mahoney (type 1), MEF (type 2), and Saukett (type 3)-as well as for attenuated oral polio vaccines (OPV) and Sabin types 1, 2, and 3. A method for analyzing biological hazardous components (biological safety level 2) was set up for the CIEF-WCID analyzer used. This method is based on closed circuits. The determined pI's were 6.2 for Mahoney, 6.7 for MEF-1, and 5.8 for Saukett. The pI's of Sabin types 1, 2, and 3 viruses were 7.4, 7.2, and 6.3, respectively. Resolution of the virus peaks was shown to be reproducible. Using this adjusted CIEF-WCID technique, the pI of biologically hazardous components like toxins or viruses can be determined, which is beneficial for the development of vaccine production methods among others.
authors.affiliation: Institute for Translational Vaccinology, P.O. Box 450, 3720 AL Bilthoven, The Netherlands. yvonne.thomassen@intravacc.nl
authors.forename: Yvonne E
authors.initials: YE
authors.lastname: Thomassen
authors.forename: Gerco
authors.initials: G
authors.lastname: van Eikenhorst
authors.forename: Leo A
authors.initials: LA
authors.lastname: van der Pol
authors.forename: Wilfried A M
authors.initials: WA
authors.lastname: Bakker
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002522:Chlorocebus aethiops; D019075:Electrophoresis, Capillary / Q000379:methods; D006367:HeLa Cells; D006801:Humans; D007525:Isoelectric Focusing / Q000379:methods; D007526:Isoelectric Point; D017955:Poliovirus / Q000737:chemistry / Q000302:isolation & purification*; D014709:Vero Cells