title: Characteristics of pigeon paramyxovirus serotype-1 isolates (PPMV-1) from the Russian Federation from 2001 to 2009.
abstract: Monitoring programs for highly dangerous avian diseases in the Russian Federation from 2001 to 2009 detected 77 samples that were PCR positive for avian paramyxovirus serotype-1 (APMV-1) from sick or dead feral and domestic pigeons. Nucleotide sequences of the fusion (F) gene, including a nucleotide sequence encoding the F protein cleavage site, were determined for these isolates. All of the studied isolates possessed virulent F0 protein cleavage sites (112KRKKRF117, 112RRQKRF117, or 112KRQKRF117). Intracerebral pathogenicity index (ICPI) values determined for seven of the isolates exceeded the value of 0.7 (the range from 0.8 to 1.41). Based on partial genome sequencing and phylogenetic analysis, the isolates were assigned to two individual sublineages within class II genotype VIb. It was determined that most of these Newcastle disease virus isolates (70/77) recovered from the pigeons belonged to a relatively poorly studied sublineage VIb/2. The complete nucleotide sequence of the genome for the Pigeon/Russia/Vladimir/687/05 isolate of sublineage VIb/2 was determined.
authors.affiliation: Federal Governmental Institution, Federal Centre for Animal Health, FGI ARRIAH, Vladimir 600901, Russia. Ipchelkina@ARRIAH.RU
authors.forename: I P
authors.initials: IP
authors.lastname: Pchelkina
authors.forename: T B
authors.initials: TB
authors.lastname: Manin
authors.forename: S N
authors.initials: SN
authors.lastname: Kolosov
authors.forename: S K
authors.initials: SK
authors.lastname: Starov
authors.forename: A V
authors.initials: AV
authors.lastname: Andriyasov
authors.forename: I A
authors.initials: IA
authors.lastname: Chvala
authors.forename: V V
authors.initials: VV
authors.lastname: Drygin
authors.forename: Q
authors.initials: Q
authors.lastname: Yu
authors.forename: P J
authors.initials: PJ
authors.lastname: Miller
authors.forename: D L
authors.initials: DL
authors.lastname: Suarez
mesh_terms: D000818:Animals; D010856:Columbidae; D005814:Genes, Viral; D008969:Molecular Sequence Data; D009521:Newcastle Disease / Q000821:virology*; D009522:Newcastle disease virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012426:Russia; D020539:Sequence Analysis, Protein / Q000662:veterinary; D017423:Sequence Analysis, RNA / Q000662:veterinary; D017385:Sequence Homology; D014774:Virulence