title: In vitro cytotoxic activity of seed oil of fenugreek against various cancer cell lines.
abstract: In the present study, investigations were carried out to screen the anticancer activities of fenugreek seed oil against cancer cell lines (HEp-2, MCF-7, WISH cells), and a normal cell line (Vero cells). Cytotoxicity was assessed with MTT and NRU assays, and cellular morphological alterations were studied using phase contrast light microscopy. All cells were exposed toi 10-1000 μg/ml of fenugreek seed oil for 24 h. The results show that fenugreek seed oil significantly reduced the cell viability, and altered the cellular morphology in a dose dependent manner. Among the cell lines, HEp-2 cells showed the highest decrease in cell viability, followed by MCF-7, WISH, and Vero cells by MTT and NRU assays. Cell viability at 1000 μg/ml was recorded as 55% in HEp-2 cells, 67% in MCF-7 cells, 75% in WISH cells, and 86% in Vero cells. The present study provides preliminary screening data for fenugreek seed oil pointing to potent cytotoxicity against cancer cells.
authors.affiliation: Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Mai Mohammad
authors.initials: MM
authors.lastname: Al-Oqail
authors.forename: Nida Nayyar
authors.initials: NN
authors.lastname: Farshori
authors.forename: Ebtesam Saad
authors.initials: ES
authors.lastname: Al-Sheddi
authors.forename: Javed
authors.initials: J
authors.lastname: Musarrat
authors.forename: Abdulaziz Ali
authors.initials: AA
authors.lastname: Al-Khedhairy
authors.forename: Maqsood Ahmed
authors.initials: MA
authors.lastname: Siddiqui
mesh_terms: D000650:Amnion / Q000166:cytology / Q000187:drug effects*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms / Q000188:drug therapy / Q000473:pathology*; D002294:Carcinoma, Squamous Cell / Q000188:drug therapy / Q000473:pathology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D005260:Female; D006801:Humans; D061986:MCF-7 Cells; D010938:Plant Oils / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry*; D029910:Trigonella / Q000737:chemistry*; D014709:Vero Cells
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010938:Plant Oils