title: Isolation and characterization of SSEA3(+) stem cells derived from goat skin fibroblasts.
abstract: Novel stem cells expressing stage-specific embryonic antigen 3 (SSEA-3) reside among human dermal fibroblasts and are known as multilineage-differentiating stress-enduring (Muse) cells. They enhance the generation efficiency of induced pluripotent stem cells. However, Muse cells have only been found in humans. We aimed to isolate SSEA3-positive cells from terminally differentiated skin fibroblasts of adult goat and determine their pluripotency. Cell clusters from SSEA3(+) populations possessed stem cell-like morphological features and normal karyotypes, were consistently positive for alkaline phosphatase, and expressed stem cell pluripotency markers. These SSEA3(+) cells remained undifferentiated over eight passages in suspension culture and were able to differentiate into cells of all three germ layers in vitro and in vivo. Our combined findings suggest that a subset of adult stem cells expressing SSEA3 also exist among adult goat skin fibroblasts. We are the first to report that multipotent adult goat cells exist among terminally differentiated goat skin in suspension culture. Our results also provide a promising platform for generation of a transgenic goat, because the undifferentiated state of stem cells was thought to be more efficient as donor cells for somatic cell nuclear transfer.
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Zhongcai
authors.initials: Z
authors.lastname: Yang
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Hongliang
authors.initials: H
authors.lastname: Liu
authors.forename: Mingning
authors.initials: M
authors.lastname: Qiu
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Liu
authors.forename: Liming
authors.initials: L
authors.lastname: Zheng
authors.forename: Meijun
authors.initials: M
authors.lastname: Pang
authors.forename: Fusheng
authors.initials: F
authors.lastname: Quan
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D030801:Animals, Genetically Modified; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000166:cytology*; D006041:Goats; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism*; D012867:Skin / Q000166:cytology*; D055746:Stage-Specific Embryonic Antigens / Q000378:metabolism*
chemical_list: D015295:Antigens, Tumor-Associated, Carbohydrate; D015415:Biomarkers; D055746:Stage-Specific Embryonic Antigens; C052924:stage-specific embryonic antigen-3; D000469:Alkaline Phosphatase