title: A new antimicrobial and anticancer peptide producing by the marine deep sediment strain "Paenibacillus profundus" sp. nov. Sl 79.
abstract: A new linear glyceryl acid derived heptapeptide (1), together with known isocoumarin antibiotic, Y-05460M-A (2), were isolated from the culture of the deep sea sediment strain SI 79 classified as "Paenibacillus profundus" sp. nov. Their structures were determined by 1D- and 2D- NMR techniques and ESI-MS/MS experiments. HPLC analysis of the Marfey derivatives in comparison to their analogs of authentic amino acids revealed that all amino acids in peptide 1, with an exception of Val, have the D-configuration. The compound 1 showed inhibitory activity against Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis, Enterococcus faecium as well as cytotoxic and moderate colony growth inhibitory activity against SK-MEL-28 cell line.
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, Pr. 100-letiya Vladivostoka 159, Russian Federation. schuka@piboc.dvo.ru
authors.forename: Nataliya I
authors.initials: NI
authors.lastname: Kalinovskaya
authors.forename: Lyudmila A
authors.initials: LA
authors.lastname: Romanenko
authors.forename: Anatoly I
authors.initials: AI
authors.lastname: Kalinovsky
authors.forename: Pavel S
authors.initials: PS
authors.lastname: Dmitrenok
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Dyshlovoy
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D001412:Bacillus subtilis / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D016984:Enterococcus faecium / Q000187:drug effects; D019015:Geologic Sediments / Q000382:microbiology*; D006801:Humans; D056507:Paenibacillus / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects; D053719:Tandem Mass Spectrometry
chemical_list: D000900:Anti-Bacterial Agents; D000972:Antineoplastic Agents, Phytogenic