title: Biotechnology advances: a perspective on the diagnosis and research of Rabies Virus.
abstract: Rabies is a widespread zoonotic disease responsible for approximately 55,000 human deaths/year. The direct fluorescent antibody test (DFAT) and the mouse inoculation test (MIT) used for rabies diagnosis, have high sensitivity and specificity, but are expensive and time-consuming. These disadvantages and the identification of new strains of the virus encourage the use of new techniques that are rapid, sensitive, specific and economical for the detection and research of the Rabies Virus (RABV). Real-time RT-PCR, phylogeographic analysis, proteomic assays and DNA recombinant technology have been used in research laboratories. Together, these techniques are effective on samples with low virus titers in the study of molecular epidemiology or in the identification of new disease markers, thus improving the performance of biological assays. In this context, modern advances in molecular technology are now beginning to complement more traditional approaches and promise to revolutionize the diagnosis of rabies. This brief review presents some of the recent molecular tools used for RABV analysis, with emphasis on rabies diagnosis and research.
authors.affiliation: Pasteur Institute, São Paulo, Brazil. sandrianabr@gmail.com
authors.forename: S R
authors.initials: SR
authors.lastname: Silva
authors.forename: I S S
authors.initials: IS
authors.lastname: Katz
authors.forename: E
authors.initials: E
authors.lastname: Mori
authors.forename: P
authors.initials: P
authors.lastname: Carnieli
authors.forename: L F P
authors.initials: LF
authors.lastname: Vieira
authors.forename: H B C R
authors.initials: HB
authors.lastname: Batista
authors.forename: L B
authors.initials: LB
authors.lastname: Chaves
authors.forename: K C
authors.initials: KC
authors.lastname: Scheffer
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001709:Biotechnology / Q000379:methods* / Q000639:trends; D005455:Fluorescent Antibody Technique; D006801:Humans; D051379:Mice; D017720:Molecular Epidemiology / Q000379:methods* / Q000639:trends; D011818:Rabies / Q000175:diagnosis / Q000453:epidemiology / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D011820:Rabies virus / Q000235:genetics / Q000276:immunology / Q000378:metabolism
chemical_list: D015415:Biomarkers
keywords: Monoclonal/recombinant antibody; Phylogeography; Proteomic; Rabies; Real-time RT-PCR