title: Presence of adenovirus species C in infiltrating lymphocytes of human sarcoma.
abstract: Human adenoviruses are known to persist in T-lymphocytes of tonsils, adenoids and intestinal tract. The oncogenic potential of different adenovirus types has been widely studied in rodents, in which adenovirus inoculation can induce multiple tumors such as undifferentiated sarcomas, adenocarcinomas and neuroectodermal tumors. However, the oncogenic potential of this virus has never been proven in human subjects. Using a highly sensitive broad-spectrum qRT-PCR, we have screened a set of different human sarcomas including leiomyosarcoma, liposarcoma and gastro intestinal stroma tumors. Primers binding the viral oncogene E1A and the capsid-coding gene Hexon were used to detect the presence of adenovirus DNA in tumor samples. We found that 18% of the tested leiomyosarcomas and 35% of the liposarcomas were positive for the presence of adenovirus DNA, being species C types the most frequently detected adenoviruses. However, only in one sample of the gastro intestinal stroma tumors the virus DNA could be detected. The occurrence of adenovirus in the tumor sections was confirmed by subsequent fluorescence in-situ-hybridization analysis and co-staining with the transcription factor Bcl11b gives evidence for the presence of the virus in infiltrating T-lymphocytes within the tumors. Together these data underline, for the first time, the persistence of adenovirus in T-lymphocytes infiltrated in muscular and fatty tissue tumor samples. If an impaired immune system leads to the viral persistence and reactivation of the virus is involved in additional diseases needs further investigation.
authors.affiliation: Leibniz Institute for Experimental Virology, Department of Molecular Virology, Hamburg, Germany.
authors.forename: Karin
authors.initials: K
authors.lastname: Kosulin
authors.forename: Franziska
authors.initials: F
authors.lastname: Hoffmann
authors.forename: Till Sebastian
authors.initials: TS
authors.lastname: Clauditz
authors.forename: Waldemar
authors.initials: W
authors.lastname: Wilczak
authors.forename: Thomas
authors.initials: T
authors.lastname: Dobner
mesh_terms: D017500:Adenovirus E1A Proteins / Q000235:genetics; D000260:Adenoviruses, Human / Q000502:physiology*; D001483:Base Sequence; D054884:Host-Pathogen Interactions; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007890:Leiomyosarcoma / Q000276:immunology / Q000473:pathology / Q000821:virology*; D008080:Liposarcoma / Q000276:immunology / Q000473:pathology / Q000821:virology*; D025202:Molecular Diagnostic Techniques; D008969:Molecular Sequence Data; D058889:Molecular Typing; D060888:Real-Time Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013601:T-Lymphocytes / Q000821:virology*; D019562:Viral Load
chemical_list: D017500:Adenovirus E1A Proteins