title: α-Fucosidase as a novel convenient biomarker for cellular senescence.
abstract: Due to its role in aging and antitumor defense, cellular senescence has recently attracted increasing interest. However, there is currently no single specific marker that can unequivocally detect senescent cells. Here, we identified α-L-fucosidase (α-Fuc) as a novel sensitive biomarker for cellular senescence. Regardless of the stress stimulus and cell type, α-Fuc activity was induced in all canonical types of cellular senescence, including replicative, DNA damage- and oncogene-induced senescence. Strikingly, in most models the degree of α-Fuc upregulation was higher than the induction of senescence-associated β-galactosidase (SA-β-Gal), the current gold standard for senescence detection. As α-Fuc is convenient and easy to measure, we suggest its utility as a valuable marker, in particular in cells with low SA-β-Gal activity.
authors.affiliation: Interfaculty Institute for Biochemistry, University of Tübingen, Tübingen, Germany.
authors.forename: Dominic G
authors.initials: DG
authors.lastname: Hildebrand
authors.forename: Simon
authors.initials: S
authors.lastname: Lehle
authors.forename: Andreas
authors.initials: A
authors.lastname: Borst
authors.forename: Sebastian
authors.initials: S
authors.lastname: Haferkamp
authors.forename: Frank
authors.initials: F
authors.lastname: Essmann
authors.forename: Klaus
authors.initials: K
authors.lastname: Schulze-Osthoff
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000235:genetics / Q000502:physiology*; D045325:HCT116 Cells; D006801:Humans; D007150:Immunohistochemistry; D008813:Mice, Inbred ICR; D060888:Real-Time Polymerase Chain Reaction; D005644:alpha-L-Fucosidase / Q000235:genetics / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D005644:alpha-L-Fucosidase
keywords: biomarker; cellular senescence; lysosome; α-fucosidase; β-galactosidase; β-glucuronidase