title: Diversity of bacterial isolates during full scale rotary drum composting.
abstract: Bacterial diversity of full scale rotary drum composter from biodegradable organic waste samples were analyzed through two different approaches, i.e., Culture dependent and independent techniques. Culture-dependent enumerations for indigenous population of bacterial isolates mainly total heterotrophic bacteria (Bacillus species, Pseudomonas species and Enterobacter species), Fecal Coliforms, Fecal Streptococci, Escherichia coli, Salmonella species and Shigella species showed reduction during the composting period. On the other hand, Culture-independent method using PCR amplification of specific 16S rRNA sequences identified the presence of Acinetobacter species, Actinobacteria species, Bacillus species, Clostridium species, Hydrogenophaga species, Butyrivibrio species, Pedobacter species, Empedobactor species and Flavobacterium species by sequences clustering in the phylogenetic tree. Furthermore, correlating physico-chemical analysis of samples with bacterial diversity revealed the bacterial communities have undergone changes, possibly linked to the variations in temperature and availability of new metabolic substrates while decomposing organics at different stages of composting.
authors.affiliation: Department of Civil Engineering, Indian Institute of Technology Roorkee (IITR), Roorkee 247 667, India. sonaakansha@gmail.com
authors.forename: Akansha
authors.initials: A
authors.lastname: Bhatia
authors.forename: Sangeeta
authors.initials: S
authors.lastname: Madan
authors.forename: Jitendra
authors.initials: J
authors.lastname: Sahoo
authors.forename: Muntjeer
authors.initials: M
authors.lastname: Ali
authors.forename: Ranjana
authors.initials: R
authors.lastname: Pathania
authors.forename: Absar Ahmed
authors.initials: AA
authors.lastname: Kazmi
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D044822:Biodiversity; D004867:Equipment Design; D008372:Manure; D059013:Microbial Consortia / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S; D012037:Refuse Disposal / Q000295:instrumentation* / Q000379:methods*; D012987:Soil; D012988:Soil Microbiology; D013696:Temperature; D014675:Vegetables
chemical_list: D008372:Manure; D012336:RNA, Ribosomal, 16S; D012987:Soil