title: Micelle formation of sodium hyodeoxycholate.
abstract: Sodium hyodeoxycholate (NaHDC) is the main component of hog bile salts, which play a role in the absorption of sparingly soluble materials in the intestinal solution. The biosurfactant has an amphiphilic molecular structure, similar to that of ursodeoxycholate from bear gallbladder. Micelle formation from hyodeoxycholate was studied at 308.2K using pyrene fluorescence probe to determine critical micelle concentrations (cmc) at various NaCl concentrations. The change in the fluorescence spectrum peak ratios with NaHDC concentration indicated two steps for bile salt aggregation. The first step was the formation of small micelles (cmc) at 5mM, and the second step was the formation of stable aggregates at 14 mM in aqueous solution. The aggregation of hyodeoxycholate, analyzed using the stepwise association model, was found to grow its aggregation number from 4 to 7 with increasing concentration. The aggregation number in aqueous solution was also confirmed by the static light scattering method. The average measured aggregation number of the micelles was 6.7. The micellar size was relatively small as measured by either method, but it was covered by general aggregation number of human bile salts. The degree of counterion binding to the micelles, determined using a sodium ion-selective electrode, was ca. 0.5 for the NaHDC micelles. This value was relatively high among typical bile salts. Moreover, the solubilization capacity of the NaHDC micelles was assessed using cholesterol. It became clear that NaHDC micelles hardly solubilized cholesterol compared to typical human bile salts. The maximum solubilization by NaHDC was equivalent only to that by sodium ursodeoxycholate.
authors.affiliation: Department of Physical Chemistry, Showa Pharmaceutical University, Higashi-Tamagawagakuen 3-3165, Machida, Tokyo, 194-8543, Japan. matsuoka@ac.shoyaku.ac.jp
authors.forename: Keisuke
authors.initials: K
authors.lastname: Matsuoka
authors.forename: Kaede
authors.initials: K
authors.lastname: Takagi
authors.forename: Chikako
authors.initials: C
authors.lastname: Honda
mesh_terms: D002784:Cholesterol / Q000737:chemistry; D003840:Deoxycholic Acid / Q000737:chemistry*; D007477:Ions / Q000737:chemistry; D008823:Micelles; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
chemical_list: D007477:Ions; D008823:Micelles; D003840:Deoxycholic Acid; C010471:hyodeoxycholic acid; D002784:Cholesterol
keywords: Aggregation number; Cholesterol; Cmc; Hyodeoxycholic acid; Micelle; Sodium hyodeoxycholate