title: Determination of phospholipase C activity in vitro.
abstract: Measurement of phospholipase C (PLC) activity in vitro is a valuable biochemistry technique easily applicable in samples from different organisms. It quantifies the enzymatic activity of a key protein involved in critical developmental functions in organisms such as plants, animals, and bacteria. A protocol is described which assays the formation of two main products of the PLC hydrolysis reaction on radioactively labeled phospholipid substrates.
authors.affiliation: Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán (CICY), Merida, Yucatan, Mexico.
authors.forename: S M Teresa
authors.initials: SM
authors.lastname: Hernández-Sotomayor
authors.forename: J Armando
authors.initials: JA
authors.lastname: Muñoz-Sanchez
mesh_terms: D000818:Animals; D057075:Enzyme Assays / Q000379:methods*; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000378:metabolism; D013379:Substrate Specificity; D014316:Tritium; D010738:Type C Phospholipases / Q000378:metabolism*
chemical_list: D019269:Phosphatidylinositol 4,5-Diphosphate; D014316:Tritium; D010738:Type C Phospholipases