title: Simple and fast PO-CL method for the evaluation of antioxidant capacity of hydrophilic and hydrophobic antioxidants.
abstract: A simple and fast procedure is described for evaluating the antioxidant activity of hydrophilic and hydrophobic compounds by using the peroxyoxalate-chemiluminescence (PO-CL) reaction of Bis(2,4,6-trichlorophenyl) oxalate (TCPO) with hydrogen peroxide in the presence of di(tert-butyl)2-(tert-butylamino)-5-[(E)-2-phenyl-1-ethenyl]3,4-furandicarboxylate as a highly fluorescent fluorophore. The IC50 values of the well-known antioxidants were calculated and the results were expressed as gallic equivalent antioxidant capacity (GEAC). It was found that the proposed method is free of physical quenching and oxidant interference, for this reason, proposed method is able to determine the accurate scavenging activity of the antioxidants to the free radicals. Finally, the proposed method was applied to the evaluation of antioxidant activity of complex real samples such as soybean oil and sunflower oil (as hydrophobic samples) and honey (as hydrophilic sample). To the best of our knowledge, this is the first time that total antioxidant activity can be determined directly in soybean oil, sunflower oil and honey (not in their extracts) using PO-CL reactions.
authors.affiliation: Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran. Kiomarszargoosh@yahoo.com
authors.forename: Kiomars
authors.initials: K
authors.lastname: Zargoosh
authors.forename: Yousef
authors.initials: Y
authors.lastname: Ghayeb
authors.forename: Behnaz
authors.initials: B
authors.lastname: Azmoon
authors.forename: Mohammad
authors.initials: M
authors.lastname: Qandalee
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D005456:Fluorescent Dyes / Q000737:chemistry; D005609:Free Radicals / Q000378:metabolism*; D006368:Helianthus / Q000737:chemistry; D006722:Honey / Q000032:analysis; D006861:Hydrogen Peroxide / Q000737:chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D049449:Luminescence; D008163:Luminescent Measurements / Q000191:economics / Q000379:methods*; D010070:Oxalates / Q000737:chemistry*; D010938:Plant Oils / Q000737:chemistry; D013024:Soybean Oil / Q000737:chemistry
chemical_list: D000975:Antioxidants; D005456:Fluorescent Dyes; D005609:Free Radicals; D010070:Oxalates; D010938:Plant Oils; C044622:peroxyoxalate; D013024:Soybean Oil; D006861:Hydrogen Peroxide