title: Transposon mutagenesis of Bacteroides fragilis using a mariner transposon vector.
abstract: The mariner transposon vector pYV07 was tested for use in the mutagenesis of Bacteroides fragilis 638R. The transposon vector efficiently generated mutants in B. fragilis 638R. The transposon disrupted genes were scattered throughout the genome of B. fragilis 638R. This method serves as a powerful tool to study B. fragilis.
authors.affiliation: GLAVAHCS, Los Angeles, CA 90073, USA. veereshagy@gmail.com
authors.forename: Yaligara
authors.initials: Y
authors.lastname: Veeranagouda
authors.forename: Fasahath
authors.initials: F
authors.lastname: Husain
authors.forename: Hannah M
authors.initials: HM
authors.lastname: Wexler
mesh_terms: D001441:Bacteroides fragilis / Q000235:genetics*; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins / Q000235:genetics*; D005798:Genes, Bacterial; D005822:Genetic Vectors; D016254:Mutagenesis, Insertional / Q000379:methods*; D014169:Transformation, Bacterial; D019895:Transposases / Q000235:genetics*
chemical_list: D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; C111652:mariner transposases; D019895:Transposases
keywords: Bacteroides fragilis; Mariner transposon; Mutant library; Rescue cloning; Semi random PCR; Transposon mutagenesis