title: Multidrug-resistant tet(X)-containing hospital isolates in Sierra Leone.
abstract: The tet(X) gene encodes a flavin-dependent monooxygenase that confers resistance to all clinically relevant tetracycline antibiotics including tigecycline. It has only previously been identified in environmental and non-human pathogenic bacteria. To investigate levels of multidrug resistance in Bo, Sierra Leone, a molecular epidemiological study was conducted using an antimicrobial resistance determinant microarray (ARDM), PCR and DNA sequencing. The study found that 21% of isolates from Mercy Hospital (Bo, Sierra Leone) were tet(X)-positive, all of which originated from urinary specimens. Use of molecular epidemiological surveillance tools has provided the first evidence of tet(X)-containing multidrug-resistant Gram-negative hospital isolates in a hospital in Sierra Leone.
authors.affiliation: Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, DC, USA.
authors.forename: Tomasz A
authors.initials: TA
authors.lastname: Leski
authors.forename: Umaru
authors.initials: U
authors.lastname: Bangura
authors.forename: David Henry
authors.initials: DH
authors.lastname: Jimmy
authors.forename: Rashid
authors.initials: R
authors.lastname: Ansumana
authors.forename: Stephen E
authors.initials: SE
authors.lastname: Lizewski
authors.forename: David A
authors.initials: DA
authors.lastname: Stenger
authors.forename: Chris Rowe
authors.initials: CR
authors.lastname: Taitt
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Vora
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D001424:Bacterial Infections / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial; D005798:Genes, Bacterial; D006761:Hospitals; D006801:Humans; D046228:Microarray Analysis; D017720:Molecular Epidemiology; D016133:Polymerase Chain Reaction; D012807:Sierra Leone; D013754:Tetracyclines / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D013754:Tetracyclines