title: A Single Nucleotide Polymorphism in the Phospholipase D1 Gene is Associated with Risk of Non-Small Cell Lung Cancer.
abstract: Phospholipase D (PLD) has an important role in various biological functions including vesicular transport, endocytosis, exocytosis, cell migration, and mitosis. These cellular biological processes are deregulated in the development of various human tumors. In order to explore the relationship between the PLD1 gene and risk of non-small cell lung cancer (NSCLC), single nucleotide polymorphisms (SNP) in the PLD1 exon region were surveyed in 211 NSCLC patients and 205 normal controls. In this study, we identified six SNPs at exon 23 in the PLD1 gene. Among the six SNPs, the most notable was a heterozygous A to C transition at nucleotide 2698 (A2698C, p<0.001). In addition, the genotype frequencies of A2744C (AC+CC) and A2756C (AC+CC) were associated with gender (female, A2744C and A2756C: p=0.071) in NSCLC patients. Interestingly, although the SNP A2698C did not cause change in amino acid, correlation between odd ratio of NSCLC patients and the SNP A2698C was observed to be statistically significant.
authors.affiliation: Division of Hematology-Oncology, Department of Internal Medicine, Department of Medicine, Samsung Medical Center, Sungkyunkwan University, School of Medicine, Seoul 135-710, Korea;
authors.forename: Myung-Ju
authors.initials: MJ
authors.lastname: Ahn
authors.forename: Shin-Young
authors.initials: SY
authors.lastname: Park
authors.forename: Won Kyu
authors.initials: WK
authors.lastname: Kim
authors.forename: Ju Hwan
authors.initials: JH
authors.lastname: Cho
authors.forename: Brian Junho
authors.initials: BJ
authors.lastname: Chang
authors.forename: Dong Jo
authors.initials: DJ
authors.lastname: Kim
authors.forename: Jin Seok
authors.initials: JS
authors.lastname: Ahn
authors.forename: Keunchil
authors.initials: K
authors.lastname: Park
authors.forename: Joong-Soo
authors.initials: JS
authors.lastname: Han
keywords: DGGE; NSCLC; biomarker; lung cancer; phospholipase D; single nucleotide polymorphism