title: Aldosterone regulates Na(+), K(+) ATPase activity in human renal proximal tubule cells through mineralocorticoid receptor.
abstract: The mechanisms by which aldosterone increases Na(+), K(+) ATPase and sodium channel activity in cortical collecting duct and distal nephron have been extensively studied. Recent investigations demonstrate that aldosterone increases Na-H exchanger-3 (NHE-3) activity, bicarbonate transport, and H(+) ATPase in proximal tubules. However, the role of aldosterone in regulation of Na(+), K(+) ATPase in proximal tubules is unknown. We hypothesize that aldosterone increases Na(+), K(+) ATPase activity in proximal tubules through activation of the mineralocorticoid receptor (MR). Immunohistochemistry of kidney sections from human, rat, and mouse kidneys revealed that the MR is expressed in the cytosol of tubules staining positively for Lotus tetragonolobus agglutinin and type IIa sodium-phosphate cotransporter (NpT2a), confirming proximal tubule localization. Adrenalectomy in Sprague-Dawley rats decreased expression of MR, ENaC α, Na(+), K(+) ATPase α1, and NHE-1 in all tubules, while supplementation with aldosterone restored expression of above proteins. In human kidney proximal tubule (HKC11) cells, treatment with aldosterone resulted in translocation of MR to the nucleus and phosphorylation of SGK-1. Treatment with aldosterone also increased Na(+), K(+) ATPase-mediated (86)Rb uptake and expression of Na(+), K(+) ATPase α1 subunits in HKC11 cells. The effects of aldosterone on Na(+), K(+) ATPase-mediated (86)Rb uptake were prevented by spironolactone, a competitive inhibitor of aldosterone for the MR, and partially by Mifepristone, a glucocorticoid receptor (GR) inhibitor. These results suggest that aldosterone regulates Na(+), K(+) ATPase in renal proximal tubule cells through an MR-dependent mechanism.
authors.affiliation: Department of Medicine, Kidney Disease Program, University of Louisville, Louisville, KY, USA.
authors.forename: Sarah A
authors.initials: SA
authors.lastname: Salyer
authors.forename: Jason
authors.initials: J
authors.lastname: Parks
authors.forename: Michelle T
authors.initials: MT
authors.lastname: Barati
authors.forename: Eleanor D
authors.initials: ED
authors.lastname: Lederer
authors.forename: Barbara J
authors.initials: BJ
authors.lastname: Clark
authors.forename: Janet D
authors.initials: JD
authors.lastname: Klein
authors.forename: Syed J
authors.initials: SJ
authors.lastname: Khundmiri
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000450:Aldosterone / Q000494:pharmacology*; D000818:Animals; D015153:Blotting, Western; D002462:Cell Membrane; D002478:Cells, Cultured; D006801:Humans; D006868:Hydrolysis; D007124:Immunoenzyme Techniques; D007687:Kidney Tubules, Proximal / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D051381:Rats; D017207:Rats, Sprague-Dawley; D018161:Receptors, Mineralocorticoid / Q000378:metabolism*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
chemical_list: D018161:Receptors, Mineralocorticoid; D000450:Aldosterone; D000255:Adenosine Triphosphate; D000254:Sodium-Potassium-Exchanging ATPase
keywords: Aldosterone; K(+) ATPase; Mineralocorticoid receptor; Na(+); Proximal tubule; Sodium and glucocorticoid-dependent kinase