title: TET2 Expression in Bone Marrow Mononuclear Cells of Patients with Myelodysplastic Syndromes and Its Clinical Significances.
abstract: OBJECTIVE
  To investigate the expression of TET2 mRNA and protein in the bone marrow mononuclear cells (BMMNC) of patients with myelodysplastic syndrome (MDS) and its clinical significance.
  
  
  METHODS
  The expression of TET2 mRNA and protein in bone marrow mononuclear cells (BMMNC) of 32 patients with MDS and 20 healthy donors was examined by qPCR and Western blot.
  
  
  RESULTS
  The expression of TET2 mRNA in BMMNC was down-regulated in MDS patients compared with the donor group [(0.41±0.28)% vs. (1.07±0.56)%] (P<0.001). Compared with lower expression group (TET2<0.4) [(6.53±6.17)%], patients with higher expression of TET2 (≥0.4) presented significantly lower proportion of bone marrow blasts [(1.21±1.56)%] (P<0.05). The expression of TET2 mRNA in BMMNC of MDS patients was inversely correlated with malignant clone burden (r=-0.398, P<0.05) and IPSS (r=-0.412, P<0.05). The expression of TET2 protein was down-regulated in MDS patients compared with that in the donor group.
  
  
  CONCLUSIONS
  The mRNA and protein expression of TET2 in BMMNC of MDS patients is decreased, which might be useful as an important parameter for the evaluation of MDS clone burden.
authors.affiliation: Department of Hematology, Tianjin Medical University General Hospital, Tianjin 300052, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Zong-Hong
authors.initials: ZH
authors.lastname: Shao
authors.forename: Rong
authors.initials: R
authors.lastname: Fu
authors.forename: Hua-Quan
authors.initials: HQ
authors.lastname: Wang
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Li
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Wen
authors.initials: W
authors.lastname: Qu
authors.forename: Yong
authors.initials: Y
authors.lastname: Liang
authors.forename: Guo-Jin
authors.initials: GJ
authors.lastname: Wang
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Wang
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Wu
authors.forename: Hong
authors.initials: H
authors.lastname: Liu
authors.forename: Jia
authors.initials: J
authors.lastname: Song
authors.forename: Jing
authors.initials: J
authors.lastname: Guan
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Xing
keywords: TET2 gene; clinical features; myelodysplastic syndrome