title: Analysis of the role of vaccinia virus H7 in virion membrane biogenesis with an H7-deletion mutant.
abstract: Essential vaccinia virus genes are often studied with conditional-lethal inducible mutants. Here, we constructed a deletion mutant lacking the essential H7R gene (the ΔH7 mutant) with an H7-expressing cell line. Compared to an inducible H7 mutant, the ΔH7 mutant showed a defect at an earlier step of virion membrane biogenesis, before the development of short crescent-shaped precursors of the viral envelope. Our studies refine the role of H7 in virion membrane biogenesis and highlight the values of analyzing deletion mutants.
authors.affiliation: Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA.
authors.forename: Xiangzhi
authors.initials: X
authors.lastname: Meng
authors.forename: Xiang
authors.initials: X
authors.lastname: Wu
authors.forename: Bo
authors.initials: B
authors.lastname: Yan
authors.forename: Junpeng
authors.initials: J
authors.lastname: Deng
authors.forename: Yan
authors.initials: Y
authors.lastname: Xiang
mesh_terms: D015153:Blotting, Western; D002460:Cell Line; D003001:Cloning, Molecular; D005455:Fluorescent Antibody Technique; D017353:Gene Deletion; D006801:Humans; D008566:Membranes / Q000502:physiology / Q000821:virology*; D008856:Microscopy, Fluorescence; D009154:Mutation / Q000235:genetics; D055550:Protein Stability; D014616:Vaccinia virus / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism*; D014771:Virion / Q000502:physiology*
chemical_list: D014764:Viral Proteins