title: Human papillomavirus type 16 does not require cathepsin L or B for infection.
abstract: Cathepsin L (CatL) and cathepsin B (CatB) are lysosomal proteases that many viruses utilize for capsid disassembly. We tested whether CatL and CatB are required for infection by human papillomavirus type 16 (HPV16). CatL- and CatB-deficient mouse embryonic fibroblasts had higher levels of infection when compared with wild-type cells. Similar results were obtained in HaCaT keratinocytes treated with CatL- or CatB-specific small interfering RNA. Thus, CatL and CatB are not required for HPV16 infection but instead appear to restrict infection.
authors.affiliation: Department of Immunobiology, University of Arizona, Tucson, AZ 85721, USA.|BIO5 Institute, University of Arizona, Tucson, AZ 85721, USA.
authors.forename: Christine M
authors.initials: CM
authors.lastname: Calton
authors.affiliation: Department of Chemistry and Biochemistry, University of Arizona, Tucson, AZ 85721, USA.|BIO5 Institute, University of Arizona, Tucson, AZ 85721, USA.
authors.forename: Angela M
authors.initials: AM
authors.lastname: Schlegel
authors.affiliation: Department of Immunobiology, University of Arizona, Tucson, AZ 85721, USA.|BIO5 Institute, University of Arizona, Tucson, AZ 85721, USA.
authors.forename: Janice A
authors.initials: JA
authors.lastname: Chapman
authors.affiliation: Department of Immunobiology, University of Arizona, Tucson, AZ 85721, USA.|BIO5 Institute, University of Arizona, Tucson, AZ 85721, USA.
authors.forename: Samuel K
authors.initials: SK
authors.lastname: Campos
mesh_terms: D000818:Animals; D002401:Cathepsin B / Q000378:metabolism*; D056668:Cathepsin L / Q000378:metabolism*; D002460:Cell Line; D005260:Female; D054884:Host-Pathogen Interactions; D052162:Human papillomavirus 16 / Q000502:physiology*; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D053586:Virus Internalization
chemical_list: C533912:CTSB protein, human; D002401:Cathepsin B; C534281:CTSL protein, human; D056668:Cathepsin L