title: A Trp199Glu MauG variant reveals a role for Trp199 interactions with pre-methylamine dehydrogenase during tryptophan tryptophylquinone biosynthesis.
abstract: MauG catalyzes posttranslational modifications of a methylamine dehydrogenase precursor (preMADH) to complete the biosynthesis of its protein-derived tryptophan tryptophylquinone (TTQ) cofactor. Trp199 is present at the site of interaction between MauG and preMADH and is critical to this process as it mediates hole hopping during the inter-protein electron transfer that is required for catalysis. Trp199 was converted to Glu and the structure and reactivity of the W199E/preMADH complex were characterized. The results reveal that the nature of residue 199 is also important for productive complex formation between preMADH and MauG.
authors.affiliation: Biochemistry and Physiology Department, College of Medicine, The University of Jordan, Amman 11942, Jordan.
authors.forename: Nafez
authors.initials: N
authors.lastname: Abu Tarboush
authors.forename: Lyndal M R
authors.initials: LM
authors.lastname: Jensen
authors.forename: Carrie M
authors.initials: CM
authors.lastname: Wilmot
authors.forename: Victor L
authors.initials: VL
authors.lastname: Davidson
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016227:Benzoquinones / Q000378:metabolism; D004579:Electron Transport; D004792:Enzyme Precursors / Q000378:metabolism*; D045563:Indolequinones / Q000096:biosynthesis*; D007501:Iron / Q000378:metabolism; D008958:Models, Molecular; D016297:Mutagenesis, Site-Directed; D009154:Mutation; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000737:chemistry / Q000378:metabolism*; D010231:Paracoccus denitrificans / Q000201:enzymology; D011485:Protein Binding; D011487:Protein Conformation; D014364:Tryptophan / Q000031:analogs & derivatives* / Q000096:biosynthesis / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D016227:Benzoquinones; D004792:Enzyme Precursors; D045563:Indolequinones; C070569:tryptophan tryptophylquinone; C004532:quinone; D014364:Tryptophan; D007501:Iron; C021567:methylamine dehydrogenase; D000587:Oxidoreductases Acting on CH-NH Group Donors