title: microRNA-330 inhibits cell motility by downregulating Sp1 in prostate cancer cells.
abstract: microRNAs (miRNAs), small non-coding RNAs, have emerged as key regulators of a large number of genes. The present study aimed to explore novel biological functions of miR-330 in the human prostate cancer cell lines DU145 and PC3. We confirmed that miR-330 was downregulated and inversely correlated with specificity protein 1 (Sp1) expression. Overexpression of miR-330 by transfection of a chemically synthesized miR-330 mimic induced a reduction in expression levels of the Sp1 protein, accompanied by significant suppression of cellular migration and invasion capability. In addition, the Sp1-knockdown experiments presented similar phenomena. Finally, the luciferase reporter assay validated Sp1 as the direct target of miR-330. These findings indicate that miR-330 acts as an anti-metastatic miRNA in prostate cancer.
authors.affiliation: Department of Urology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
authors.forename: Yeqing
authors.initials: Y
authors.lastname: Mao
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.forename: Yiwei
authors.initials: Y
authors.lastname: Lin
authors.forename: Xin
authors.initials: X
authors.lastname: Xu
authors.forename: Zhenghui
authors.initials: Z
authors.lastname: Hu
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhu
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.forename: Xianglai
authors.initials: X
authors.lastname: Xu
authors.forename: Xiangyi
authors.initials: X
authors.lastname: Zheng
authors.forename: Liping
authors.initials: L
authors.lastname: Xie
mesh_terms: D017209:Apoptosis / Q000235:genetics; D002453:Cell Cycle; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000235:genetics; D009362:Neoplasm Metastasis / Q000235:genetics; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism*; D034622:RNA Interference; D034741:RNA, Small Interfering; D016329:Sp1 Transcription Factor / Q000096:biosynthesis* / Q000235:genetics
chemical_list: C570805:MIRN330 microRNA, human; D035683:MicroRNAs; D034741:RNA, Small Interfering; D016329:Sp1 Transcription Factor