title: Sonic hedgehog inhibition induces mouse embryonic stem cells to differentiate toward definitive endoderm.
abstract: In the experimental group (shh inhibited group), there were significant decreases in the expression of Oct4, Nanog, Shh, GATA4, Brachyury and Goosecoid, while increases were observed for TAT and Pdx1. The expression of Sox17 did not differ between two control and experimental groups. In experimental group, the amount of GSC positive cells was somehow lower but it seems that there was no difference for Sox17. Shh inhibition induces ESCs to differentiate toward definitive endoderm by committing mesendodermal lineages.
authors.affiliation: Fertility and Infertility Research Center, Kermanshah University of Medical Sciences, Kermanshah, P.O. Box 1568, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Ghanbari
authors.forename: Mozafar
authors.initials: M
authors.lastname: Khazaei
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Hashemi-Tabar
authors.forename: Arezou
authors.initials: A
authors.lastname: Rabzia
authors.forename: Fardin
authors.initials: F
authors.lastname: Fathi
authors.forename: Parvin-Dokht
authors.initials: PD
authors.lastname: Bayat
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002460:Cell Line; D019070:Cell Lineage; D017931:DNA Primers; D004230:Dithizone / Q000494:pharmacology; D053595:Embryonic Stem Cells / Q000166:cytology*; D004707:Endoderm / Q000378:metabolism*; D018507:Gene Expression Regulation, Developmental; D053823:Hedgehog Proteins / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000378:metabolism; D007150:Immunohistochemistry; D008648:Mesoderm / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D053823:Hedgehog Proteins; D018398:Homeodomain Proteins; D050814:Octamer Transcription Factor-3; C465570:Shh protein, mouse; D004230:Dithizone