title: Comparative proteomics and correlated signaling network of kidney in ApoE deficient mouse.
abstract: OBJECTIVE
  Apolipoprotein E knockout (apoE-/- ) mouse is one of the most popular models for cardiovascular research, especially in the study of atherosclerosis. Naturally, large amount of studies try to uncover the role of apoE in atherosclerosis, and indeed apoE plays an important role in this pathogenesis. Kidney is an organ that contains lots of capillaries and also largely expresses apoE. Moreover, a protective role of apoE in kidney as an autocrine regulator has been demonstrated previously, however, the underlying mechanism is largely unknown.
  
  
  METHODS
  In this study, comparative proteomics is for the first time used to identify the differential proteins in kidneys of apoE-/- and wild type (WT) mice, respectively, and we try to reveal the signaling network of apoE in mice kidney using bioinformatics analysis.
  
  
  RESULTS
  Our findings show that approximately 80 proteins are significantly differentially expressed in kidneys of apoE-/- and WT mice, and the signaling network correlated to apoE is successfully established by employing bioinformatics assay.
  
  
  CONCLUSIONS
  Taken together, we originally identify the proteins with differential expression and propose an apoE correlated molecular network in mice kidney. These findings further provide evidence of the role of apoE in mice kidney and a brand new perspective in the protection and treatment of kidney disease.
authors.affiliation: 1Department of Dermatology, West China Hospital, West China Medical School, Sichuan University, Chengdu, 610041, P.R. China; Core Facility of Genetically Engineered Mice, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, 610041, P.R. China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Lv
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Ai
authors.forename: Mi
authors.initials: M
authors.lastname: Li
authors.forename: Honglian
authors.initials: H
authors.lastname: Wang
authors.forename: Tielin
authors.initials: T
authors.lastname: Chen
authors.forename: Yin
authors.initials: Y
authors.lastname: Fang
authors.forename: Yunhong
authors.initials: Y
authors.lastname: Liu
authors.forename: Puhui
authors.initials: P
authors.lastname: Zhou
authors.forename: Mianzhi
authors.initials: M
authors.lastname: Chen
authors.forename: Ruizhi
authors.initials: R
authors.lastname: Tan
authors.forename: Yuhang
authors.initials: Y
authors.lastname: Liu
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Qin
authors.initials: Q
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001057:Apolipoproteins E / Q000172:deficiency* / Q000378:metabolism; D016000:Cluster Analysis; D015180:Electrophoresis, Gel, Two-Dimensional; D005786:Gene Expression Regulation; D005838:Genotype; D007668:Kidney / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D040901:Proteomics / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D001057:Apolipoproteins E; D012333:RNA, Messenger