title: Molecular characterization, expression patterns, and association analysis with carcass traits of porcine USF1 gene.
abstract: The upstream stimulatory factor 1 (USF1) is a transcription factor controlling expression of several genes involved in lipid and glucose homeostasis. In this study, two isoforms of the porcine USF1 gene were detected by reverse transcription polymerase chain reaction (RT-PCR), termed USF1 wild-type (wt) and USF1/CD, both of them contain a helix-loop-helix leucine zipper (HLH-LZ) conserved domain. Tissue distribution analysis showed that the two transcripts of porcine USF1 gene were ubiquitously expressed in all tested tissues, except for heart. Moreover, we found that a single nucleotide polymorphism (SNP, C/T) in intron 10 was significantly associated with ratio of lean to fat (P < 0.05), dress percentage (P < 0.05), average backfat thickness (P < 0.05), loin eye width (P < 0.05), lean meat percentage (P < 0.01), loin eye height (P < 0.01), and loin eye area (P < 0.01). This result suggests that porcine USF1 gene may be a candidate gene of meat production trait.
authors.affiliation: Hubei Key Laboratory of Animal Embryo and Molecular Breeding, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan 430064, China.
authors.forename: Huayu
authors.initials: H
authors.lastname: Wu
authors.forename: Mu
authors.initials: M
authors.lastname: Qiao
authors.forename: Xianwen
authors.initials: X
authors.lastname: Peng
authors.forename: Junjing
authors.initials: J
authors.lastname: Wu
authors.forename: Guisheng
authors.initials: G
authors.lastname: Liu
authors.forename: Hua
authors.initials: H
authors.lastname: Sun
authors.forename: Lianghua
authors.initials: L
authors.lastname: Li
authors.forename: Shuqi
authors.initials: S
authors.lastname: Mei
mesh_terms: D000818:Animals; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics*; D056726:Genetic Association Studies; D009928:Organ Specificity; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D013552:Swine; D014018:Tissue Distribution; D051720:Upstream Stimulatory Factors / Q000235:genetics* / Q000378:metabolism*
chemical_list: D051720:Upstream Stimulatory Factors