title: The crystal structure of acidic β-galactosidase from Aspergillus oryzae.
abstract: The crystal structure of the industrially important Aspergillus oryzae β-galactosidase has been determined at 2.60 Å resolution. The Ao-β-gal is a large (985 residues) monomeric multi-domain enzyme that has a catalytic (α/β)8-barrel domain. An electron density map revealed extensive N-glycosylation between the domain interfaces suggesting that the oligosaccharide-chains would have a stabilizing role for the structure of Ao-β-gal. Comparison of structure with other β-galactosidase structures of glycoside hydrolase family 35 revealed a number of hydrophobic residues, which may contribute favorably to the stabilization of the structure. The role of a high number of acidic residues in Ao-β-gal is also discussed.
authors.affiliation: Department of Chemistry, University of Eastern Finland, P.O. Box 111, 80100 Joensuu, Finland.
authors.forename: Mirko M
authors.initials: MM
authors.lastname: Maksimainen
authors.forename: Anja
authors.initials: A
authors.lastname: Lampio
authors.forename: Mirka
authors.initials: M
authors.lastname: Mertanen
authors.forename: Ossi
authors.initials: O
authors.lastname: Turunen
authors.forename: Juha
authors.initials: J
authors.lastname: Rouvinen
mesh_terms: D000595:Amino Acid Sequence; D001236:Aspergillus oryzae / Q000201:enzymology*; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D005656:Fungal Proteins / Q000737:chemistry* / Q000378:metabolism; D006031:Glycosylation; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D016415:Sequence Alignment; D001616:beta-Galactosidase / Q000737:chemistry* / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; C021502:acid beta-galactosidase; D001616:beta-Galactosidase
keywords: 2-methyl-2,4-pentanediol; Ao-β-gal; Aspergillus oryzae; Glycoside hydrolase; MPD; N-glycosylation; PDB; Protein Data Bank; Psp-β-gal; Tr-β-gal; crystal structure; β-galactosidase; β-galactosidase from Aspergillus oryzae; β-galactosidase from Penicllium sp.; β-galactosidase from Trichoderma reesei