title: Comparison of multilocus sequence analysis and virulence genotyping of Escherichia coli from live birds, retail poultry meat, and human extraintestinal infection.
abstract: To examine the correlations between virulence genotyping and multilocus sequence analysis of Escherichia coli from poultry and humans, 88 isolates were examined. The isolates were selected from a population of over 1000 based on their assignment to nine different virulence genotyping clusters. Clustering based on multilocus sequence analysis mostly correlated with virulence genotyping, although multilocus sequence analysis demonstrated higher discriminatory ability and greater reliability related to inferred phylogenetic relationships. No distinct patterns in host source were observed using inferred phylogeny through multilocus sequence analysis, indicating that human, avian, and retail meat isolates are diverse, and some belong to multiple shared clonal complexes. Clonal complexes with host source overlap included ST95 and ST23 and additional novel groups, underscoring the diversity of avian pathogenic E. coli and the potential importance of these novel groups as avian and zoonotic pathogens.
authors.affiliation: Department of Veterinary and Biomedical Sciences, University of Minnesota, St. Paul, MN 55108, USA.
authors.forename: Jessica L
authors.initials: JL
authors.lastname: Danzeisen
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Wannemuehler
authors.forename: Lisa K
authors.initials: LK
authors.lastname: Nolan
authors.forename: Timothy J
authors.initials: TJ
authors.lastname: Johnson
mesh_terms: D000818:Animals; D002645:Chickens; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity*; D004927:Escherichia coli Infections / Q000382:microbiology* / Q000662:veterinary*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D060005:Genotyping Techniques / Q000662:veterinary; D006801:Humans; D008460:Meat / Q000382:microbiology*; D008969:Molecular Sequence Data; D058885:Multilocus Sequence Typing / Q000662:veterinary; D010802:Phylogeny; D011201:Poultry Diseases / Q000382:microbiology*; D015203:Reproducibility of Results; D017385:Sequence Homology; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D037521:Virulence Factors