title: The sequences of the eleven-residue peptaibiotics: suzukacillins-B.
abstract: The filamentous fungus designated 'Trichoderma viride' strain 63 C-1 simultaneously produces suzukacillins (SZs), two microheterogeneous groups of peptaibols, under submerged culture conditions. Both groups are readily distinguishable by TLC: the major group is designated SZ-A, whereas the minor group with a higher Rf value is named SZ-B. The peptide mixture was obtained from a MeOH extract of the mycelium. SZ-B was separated from SZ-A by Sephadex LH-20 column chromatography. Although it provided one single spot on silica-gel TLC plates, 15 individual peptides could be separated by C8 reversed-phase (RP) HPLC, and their sequences were determined by HPLC/QqTOF-ESI-HRMS. Fourteen peptides exhibit the C-terminal sequence Pro(6) -Lxx-Lxx-Aib-Pro-Vxxol/Lxxol(11) , which is common for eleven-residue peptaibols. The remaining peptide is tentatively assigned as a ten-residue sequence, in which the C-terminal 1,2-amino alcohol is deleted, thus terminating in free proline. Nine of the peptides carry an Ac-Aib residue at the N-terminus, very frequently found in eleven-residue peptaibols. Four peptides comprise the rare Ac-Ala N-terminus, and for two peptides, N-terminal Ac-D-Iva residues were identified. One peptide contains a C-terminal residue of yet undetermined structure. Comparison with previously reported eleven-residue peptaibol sequences reveals that eight of the peptides represent new sequence analogs.
authors.affiliation: Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Bioresources Project Group, Winchesterstrasse 2, D-35394 Giessen. christian.roehrich@ime.fraunhofer.de
authors.forename: Christian René
authors.initials: CR
authors.lastname: Röhrich
authors.forename: Andreas
authors.initials: A
authors.lastname: Vilcinskas
authors.forename: Hans
authors.initials: H
authors.lastname: Brückner
authors.forename: Thomas
authors.initials: T
authors.lastname: Degenkolb
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D046508:Culture Techniques; D008969:Molecular Sequence Data; D054848:Peptaibols / Q000737:chemistry* / Q000235:genetics; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification; D014242:Trichoderma / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D054848:Peptaibols; D010455:Peptides; C016123:suzukacillin