title: Retrograde tracing technique for neonatal animals.
abstract: Tract tracing is a fundamental technique in neuroanatomy for examining fiber connections in the nervous system. After the introduction of horseradish peroxidase 40 years ago, many tracing substances have been used for neuroanatomical studies on various nervous systems. Here, we described retrograde tracing techniques using multiple fluorescent tracers, which make it possible to detect axonal collaterals. This technique is useful to study the development of axonal trajectories, as well as regenerative and compensatory mechanisms of animals that undergo neural damage at early stages.
authors.affiliation: Department of Neuroanatomy, Yokohama City University Graduate School of Medicine, Yokohama, Japan.
authors.forename: Kengo
authors.initials: K
authors.lastname: Funakoshi
authors.forename: Akira
authors.initials: A
authors.lastname: Yoshikawa
authors.forename: Yoshitoshi
authors.initials: Y
authors.lastname: Atobe
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002540:Cerebral Cortex / Q000166:cytology; D007267:Injections; D008856:Microscopy, Fluorescence; D056347:Neuroanatomical Tract-Tracing Techniques / Q000379:methods*; D010477:Perfusion; D013194:Staining and Labeling; D016707:Tissue Fixation