title: [Detection of fps tumor antigen with mono-specific anti-fps serum in tumors induced by acute transforming ALV].
abstract: OBJECTIVE
  To prepare anti-fps mono-specific serum, and detect the fps antigen in tumors induced by acute transforming avian leukosis/sarcoma virus containing v-fps oncogene.
  
  
  METHODS
  Two part of v-fps gene was amplified by RT-PCR using the Fu-J viral RNA as the template. Mono-specific serum was prepared by immuning Kunming white mouse with both two recombinant infusion proteins expressed by the prokaryotic expression system. Indirect immunofluorescent assay was used to detect fps antigen in tumor tissue suspension cells and CEF infected by sarcoma supernatant. Immunohistochemical method was used to detect fps antigen in tumor tissue.
  
  
  RESULTS
  The mouse mono-specific serum was specific as it had no cross reaction with classical ALV-J strains. The result reveals that the tumor tissue suspension cells, the CEF infected by sarcoma supernatant, and the slice immunohistochemistry of the sarcoma showed positive results.
  
  
  CONCLUSIONS
  The anti-fps mono-specific serum was prepared, and the detection method was established, which laid the foundation for the study of viral biological characteristics and mechanism of tumourgenesis of acute transforming avian leukosis/sarcoma virus containing v-fps oncogene.
authors.affiliation: Animal Science and Technology College, Shandong Agricultural University, Tai'an 271018, China. 07sdauwyx@163.com
authors.forename: Yixin
authors.initials: Y
authors.lastname: Wang
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
authors.forename: Jianliang
authors.initials: J
authors.lastname: Li
authors.forename: Zhizhong
authors.initials: Z
authors.lastname: Cui
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D000918:Antibody Specificity; D000951:Antigens, Neoplasm / Q000235:genetics / Q000276:immunology; D001353:Avian Leukosis / Q000276:immunology / Q000821:virology; D001354:Avian Leukosis Virus / Q000276:immunology*; D001358:Avian Sarcoma Viruses / Q000276:immunology*; D002471:Cell Transformation, Neoplastic; D002645:Chickens; D005354:Fibrosarcoma / Q000276:immunology* / Q000821:virology; D051379:Mice; D011201:Poultry Diseases / Q000276:immunology* / Q000821:virology; D051578:Proto-Oncogene Proteins c-fes / Q000235:genetics / Q000276:immunology*; D012367:RNA, Viral / Q000235:genetics; D001357:Sarcoma, Avian / Q000276:immunology / Q000821:virology; D013047:Specific Pathogen-Free Organisms
chemical_list: D000914:Antibodies, Viral; D000951:Antigens, Neoplasm; D012367:RNA, Viral; D051578:Proto-Oncogene Proteins c-fes