title: Effect of high pressure on the structure and antibacterial activity of bovine lactoferrin treated in different media.
abstract: The effect of high-pressure treatment (400, 500 and 650 MPa) on the structure and activity of bovine lactoferrin in different iron-saturation forms has been studied by several techniques. The structural changes produced in lactoferrin by high-pressure were analysed by differential scanning calorimetry and fluorescence spectroscopy, and the immunoreactivity by ELISA. The effect of high-pressure was also studied on some biological properties of lactoferrin, such as iron binding capacity, retention of the bound iron, and antibacterial activity against Escherichia coli O157:H7. Results obtained indicate that treatment at 400 MPa does not substantially modify the conformation of lactoferrin, meanwhile treatments at 500 and 650 MPa greatly affect some of its properties. With respect to the antibacterial activity, the apo and native forms of lactoferrin maintain that activity against Esch. coli only after 400 MPa treatment.
authors.affiliation: Tecnología de los Alimentos, Facultad de Veterinaria, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain.
authors.forename: Indira
authors.initials: I
authors.lastname: Franco
authors.forename: Mańa D
authors.initials: MD
authors.lastname: Pérez
authors.forename: Eduardo
authors.initials: E
authors.lastname: Castillo
authors.forename: Miguel
authors.initials: M
authors.lastname: Calvo
authors.forename: Lourdes
authors.initials: L
authors.lastname: Sánchez
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D002152:Calorimetry, Differential Scanning; D002417:Cattle; D004797:Enzyme-Linked Immunosorbent Assay; D019453:Escherichia coli O157 / Q000187:drug effects; D007781:Lactoferrin / Q000737:chemistry / Q000494:pharmacology*; D008968:Molecular Conformation; D011312:Pressure; D013050:Spectrometry, Fluorescence; D013329:Structure-Activity Relationship
chemical_list: D000900:Anti-Bacterial Agents; D007781:Lactoferrin