title: Differential IL-13 production by small intestinal leukocytes in active coeliac disease versus refractory coeliac disease.
abstract: A small fraction of coeliac disease (CD) patients have persistent villous atrophy despite strict adherence to a gluten-free diet. Some of these refractory CD (RCD) patients develop a clonal expansion of lymphocytes with an aberrant phenotype, referred to as RCD type II (RCDII). Pathogenesis of active CD (ACD) has been shown to be related to gluten-specific immunity whereas the disease is no longer gluten driven in RCD. We therefore hypothesized that the immune response is differentially regulated by cytokines in ACD versus RCDII and investigated mucosal cytokine release after polyclonal stimulation of isolated mucosal lymphocytes. Secretion of the T(H)2 cytokine IL-13 was significantly higher in lamina propria leukocytes (LPLs) isolated from RCDII patients as compared to LPL from ACD patients (P = 0.05). In patients successfully treated with a gluten-free diet LPL-derived IL-13 production was also higher as compared to ACD patients (P = 0.02). IL-13 secretion correlated with other T(H)2 as well as T(H)1 cytokines but not with IL-10 secretion. Overall, the cytokine production pattern of LPL in RCDII showed more similarities with LPL isolated from GFD patients than from ACD patients. Our data suggest that different immunological processes are involved in RCDII and ACD with a potential role for IL-13.
authors.affiliation: Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands.
authors.forename: Sascha
authors.initials: S
authors.lastname: Gross
authors.forename: Roy L
authors.initials: RL
authors.lastname: van Wanrooij
authors.forename: Petula
authors.initials: P
authors.lastname: Nijeboer
authors.forename: Kyra A
authors.initials: KA
authors.lastname: Gelderman
authors.forename: Saskia A G M
authors.initials: SA
authors.lastname: Cillessen
authors.forename: Gerrit A
authors.initials: GA
authors.lastname: Meijer
authors.forename: Chris J J
authors.initials: CJ
authors.lastname: Mulder
authors.forename: Gerd
authors.initials: G
authors.lastname: Bouma
authors.forename: B Mary E
authors.initials: BM
authors.lastname: von Blomberg
authors.forename: Hetty J
authors.initials: HJ
authors.lastname: Bontkes
mesh_terms: D000328:Adult; D000368:Aged; D002446:Celiac Disease / Q000276:immunology* / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D018793:Interleukin-13 / Q000378:metabolism*; D007421:Intestine, Small / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D007962:Leukocytes / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D055815:Young Adult
chemical_list: D018793:Interleukin-13; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma