title: Genetic screening for mutations in the chip gene in intracranial aneurysm patients of Chinese Han nationality.
abstract: We performed a case-control study to investigate whether SNPs of CHIP might affect the development of IA in Chinese Han nationality. We believe we are the first to have screened IA patients for mutations in the CHIP gene to determine the association with these variants. The study group comprised 224 Chinese Han nationality patients with at least one intracranial aneurysm and 238 unrelated healthy Han nationality controls. Genomic DNA was isolated from blood leukocytes. The entire coding regions of CHIP were genotyped by PCR amplification and DNA sequencing. Differences in genotype and allele frequencies between patients and controls were tested by the chi-square method. Genotype and allele frequencies of the SNP rs116166850 was demonstrated to be in Hardy-Weinberg equilibrium. No significant difference in genotype or allele frequencies between case and control groups was detected at the SNP. Our data do not support the hypothesis of a major role for the CHIP gene in IA development in the Chinese Han population.
authors.affiliation: Department of Neurosurgery, the First Affiliated Hospital of Baotou Medical College, Baotou, China. suli297@yahoo.cn
authors.forename: Li
authors.initials: L
authors.lastname: Su
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Chun-Yang
authors.initials: CY
authors.lastname: Zhang
authors.forename: An-Long
authors.initials: AL
authors.lastname: Zhang
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Mei
authors.forename: Zhi-Jun
authors.initials: ZJ
authors.lastname: Zhao
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Han
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Zhao
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D005500:Follow-Up Studies; D005787:Gene Frequency; D005820:Genetic Testing; D005838:Genotype; D006801:Humans; D002532:Intracranial Aneurysm / Q000175:diagnosis / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009154:Mutation / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011379:Prognosis; D044767:Ubiquitin-Protein Ligases / Q000235:genetics*; D055815:Young Adult
chemical_list: C467728:STUB1 protein, human; D044767:Ubiquitin-Protein Ligases