title: Acetobacter strains isolated during the acetification of blueberry (Vaccinium corymbosum L.) wine.
abstract: Highbush blueberries (Vaccinium corymbosum L.) are known to have positive health benefits. The production of blueberry vinegar is one method to preserve this seasonal fruit and allow extended consumption. In this study, blueberry wine acetification was performed with naturally occurring micro-organisms and with an inoculated Acetobacter cerevisiae strain. Acetifications were carried out in triplicate using the Schützenbach method. The successful spontaneous processes took up to 66% more time than the processes involving inoculation. The isolation of acetic acid bacteria (AAB) and the analysis of these AAB using molecular methods allowed the identification of the main genotypes responsible of the blueberry acetification. Although the Acet. cerevisiae strain was the predominant strain isolated from the inoculated process samples, Acetobacter pasteurianus was isolated from samples for both processes and was the only species present in the spontaneous acetification samples. To the best of our knowledge, this is the first report describing the identification and variability of AAB isolated during blueberry acetification. The isolated Acet. pasteurianus strains could be used for large-scale blueberry vinegar production or as a starter culture in studies of other vinegar production methods.
authors.affiliation: Biotecnologia Enológica, Dept. Bioquímica i Biotecnologia, Facultat d'Enologia, Universitat Rovira i Virgili, Tarragona, Spain.
authors.forename: C
authors.initials: C
authors.lastname: Hidalgo
authors.forename: D
authors.initials: D
authors.lastname: García
authors.forename: J
authors.initials: J
authors.lastname: Romero
authors.forename: A
authors.initials: A
authors.lastname: Mas
authors.forename: M J
authors.initials: MJ
authors.lastname: Torija
authors.forename: E
authors.initials: E
authors.lastname: Mateo
mesh_terms: D019342:Acetic Acid / Q000737:chemistry; D000091:Acetobacter / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D029798:Blueberry Plants / Q000382:microbiology*; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005516:Food Microbiology / Q000379:methods; D005638:Fruit / Q000382:microbiology; D005838:Genotype; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014920:Wine / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer; D012336:RNA, Ribosomal, 16S; D019342:Acetic Acid
keywords: Acetobacter; Schützenbach method; highbush blueberries; starter culture; vinegar