title: Proteomic analysis of protein expression in the induction of the viable but Nonculturable State of Vibrio harveyi SF1.
abstract: Vibrio harveyi has been reported to enter into a viable but nonculturable (VBNC) state. One marine V. harveyi strain, SF1 became nonculturable when incubated in seawater microcosm at 4 °C within 60 days. We investigated protein expression in the exponential phase of V. harveyi SF1 and compared it to the VBNC state. Cytosolic proteins were resolved by two-dimensional polyacrylamide gel electrophoresis using pH 4-7 linear gradients. Among these proteins, sixteen proteins which were strongly downregulated or upregulated in the VBNC cells were identified by MALDI-TOF-TOF mass spectrometry. The results indicated that the differentially expressed proteins were mainly focused on stress response proteins and key components of central and intermediary metabolism, like carbohydrate metabolism, transport, and translation. This study provided clues for understanding the mechanism of adaptation to the VBNC state.
authors.affiliation: The Food and Agricultural Products Testing Agency, Shandong Entry-exit Inspection and Quarantine Bureau of China, Qingdao 266002, China.
authors.forename: Juntao
authors.initials: J
authors.lastname: Jia
authors.forename: Zhengyi
authors.initials: Z
authors.lastname: Li
authors.forename: Jijuan
authors.initials: J
authors.lastname: Cao
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Jiang
authors.forename: Chengzhu
authors.initials: C
authors.lastname: Liang
authors.forename: Mengzhen
authors.initials: M
authors.lastname: Liu
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D015169:Colony Count, Microbial; D003470:Culture Media / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005393:Fish Diseases / Q000382:microbiology*; D015964:Gene Expression Regulation, Bacterial; D013058:Mass Spectrometry; D050296:Microbial Viability; D008969:Molecular Sequence Data; D010473:Perciformes; D040901:Proteomics; D014733:Vibrio / Q000737:chemistry / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D014735:Vibrio Infections / Q000382:microbiology / Q000662:veterinary*
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media