title: Development of quantitative real-time PCR primers for detecting 42 oral bacterial species.
abstract: In this study, we introduced species-specific quantitative real-time PCR (qPCR) primers designed based on a DNA-dependent RNA polymerase beta-subunit gene (rpoB) for detecting 42 oral bacterial species. The specificity of the qPCR primers was confirmed by conventional PCR with the genomic DNAs of 73-79 strains regarding 73-75 bacterial species including the type strain for the target species. The standard curves revealed the lower detection limits of 42 bacterial species-specific qPCR primers ranged from 4 to 40 fg below a cycle threshold (C T) value of 35, except Atopobium rimae, Fusobacterium nucleatum, Neisseria meningitidis, and Porphyromonas asaccharolytica which were 400 fg. These results suggest that 42 bacterial species-specific qPCR primers are suitable for applications in epidemiological studies related to oral infectious diseases such as periodontal diseases, endodontic infection, and dental caries.
authors.affiliation: Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun University, 375 Seo-suk Dong, Dong-Gu, Gwangju 501-759, Republic of Korea.
authors.forename: Soon-Nang
authors.initials: SN
authors.lastname: Park
authors.forename: Yun Kyong
authors.initials: YK
authors.lastname: Lim
authors.forename: Joong-Ki
authors.initials: JK
authors.lastname: Kook
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D006801:Humans; D009055:Mouth / Q000382:microbiology*; D009059:Mouth Diseases / Q000175:diagnosis / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit