title: Conversion of carbon dioxide to oxaloacetate using integrated carbonic anhydrase and phosphoenolpyruvate carboxylase.
abstract: The development and implementation of strategies for CO2 mitigation are necessary to counteract the greenhouse gas effect of carbon dioxide emissions. To demonstrate the possibility of simultaneously capturing CO2 and utilizing four-carbon compounds, an integrated system using CA and PEPCase was developed, which mimics an in vivo carbon dioxide concentration mechanism. We first cloned the PEPCase 1 gene of the marine diatom Phaeodactylum tricornutum and produced a recombinant PtPEPCase 1. The affinity column purified PtPEPCase 1 exhibited specific enzymatic activity (5.89 U/mg). When the simultaneous and coordinated reactions of CA from Dunaliella sp. and the PtPEPCase 1 occurred, more OAA was produced than when only PEPCase was present. Therefore, this integrated CA-PEPCase system can be used not only to capture CO2 but also for a new technology to produce value-added four-carbon platform chemicals.
authors.affiliation: Department of Life Science, Research Institute for Natural Sciences, Hanyang University, Seoul, 133-791, Korea.
authors.forename: Kwang Suk
authors.initials: KS
authors.lastname: Chang
authors.forename: Hancheol
authors.initials: H
authors.lastname: Jeon
authors.forename: Man Bock
authors.initials: MB
authors.lastname: Gu
authors.forename: Seung Pil
authors.initials: SP
authors.lastname: Pack
authors.forename: EonSeon
authors.initials: E
authors.lastname: Jin
mesh_terms: D015153:Blotting, Western; D002245:Carbon Dioxide / Q000378:metabolism*; D002256:Carbonic Anhydrases / Q000235:genetics / Q000378:metabolism*; D002474:Cell-Free System; D003001:Cloning, Molecular; D017377:Diatoms / Q000201:enzymology*; D004591:Electrophoresis, Polyacrylamide Gel; D005822:Genetic Vectors; D062907:Oxaloacetic Acid / Q000378:metabolism*; D010730:Phosphoenolpyruvate Carboxylase / Q000235:genetics / Q000378:metabolism*
chemical_list: D002245:Carbon Dioxide; D062907:Oxaloacetic Acid; D010730:Phosphoenolpyruvate Carboxylase; D002256:Carbonic Anhydrases