title: Effect of silicate supplementation on the alleviation of arsenite toxicity in 93-11 (Oryza sativa L. indica).
abstract: Chronic exposure to arsenic (As) in rice has raised many health and environmental problems. As reported, great variation exists among different rice genotypes in As uptake, translocation, and accumulation. Under hydroponic culture, we find that the Chinese wild rice (Oryza rufipogon; acc. 104624) takes up the most arsenic among tested genotypes. Of the cultivated rice, the indica cv. 93-11 has the lowest arsenic translocation factor value but accumulates the maximum concentration of arsenic followed by Nipponbare, Minghui 86, and Zhonghua 11. Higher level of arsenite concentration (50 μM) can induce extensive photosynthesis and root growth inhibition, and cause severe oxidative stress. Interestingly, external silicate (Si) supplementation has significantly increased the net photosynthetic rate, and promoted root elongation, as well as strongly ameliorated the oxidative stress by increasing the activities of antioxidant enzymes superoxide dismutase, ascorbate peroxidase, and peroxidase in roots and/or leaves of 93-11 seedlings. Notably, 1.873 mM concentration of Si considerably decreases the total As uptake and As content in roots, but significantly increases the As translocation from roots to shoots. In contrast, Si supplementation with 1.0 mM concentration significantly increases the total As uptake and As concentrations in roots and shoots of 93-11 seedlings after 50 μM arsenite treatment for 6 days.
authors.affiliation: College of Agriculture and Food Science, Zhejiang A & F University, Lin'an Hangzhou, 311300, China.
authors.forename: Haichao
authors.initials: H
authors.lastname: Hu
authors.forename: Junting
authors.initials: J
authors.lastname: Zhang
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Ruochen
authors.initials: R
authors.lastname: Li
authors.forename: Fengshan
authors.initials: F
authors.lastname: Pan
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.forename: Ying
authors.initials: Y
authors.lastname: Feng
authors.forename: Yeqing
authors.initials: Y
authors.lastname: Ying
authors.forename: Qingpo
authors.initials: Q
authors.lastname: Liu
mesh_terms: D000222:Adaptation, Physiological; D000975:Antioxidants / Q000378:metabolism* / Q000494:pharmacology; D001151:Arsenic / Q000633:toxicity; D018053:Arsenites / Q000378:metabolism / Q000633:toxicity*; D060387:Ascorbate Peroxidases / Q000378:metabolism; D001673:Biodegradation, Environmental; D052918:Environmental Restoration and Remediation / Q000379:methods; D018527:Hydroponics; D012275:Oryza / Q000145:classification / Q000187:drug effects* / Q000502:physiology; D018384:Oxidative Stress / Q000187:drug effects; D010544:Peroxidases / Q000378:metabolism; D018515:Plant Leaves / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects / Q000378:metabolism; D017640:Silicates / Q000378:metabolism* / Q000494:pharmacology; D012989:Soil Pollutants / Q000378:metabolism / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D018053:Arsenites; D017640:Silicates; D012989:Soil Pollutants; D010544:Peroxidases; D060387:Ascorbate Peroxidases; D013482:Superoxide Dismutase; C015001:arsenite; D001151:Arsenic