title: Mepanipyrim haptens and antibodies with nanomolar affinity.
abstract: Mepanipyrim is an anilinopyrimidine fungicide used worldwide for crop protection. With the aim of developing useful immunoreagents for mepanipyrim immunoanalysis, two new functionalized derivatives were prepared and antibodies were generated. Affinity and specificity were assessed by direct and indirect competitive ELISA using homologous and heterologous conjugates. Although all antibodies were selective for the target analyte, the immunizing hapten structure was revealed as a determinant for high-affinity antibody production (IC(50) = 3 nM).
authors.affiliation: Institute of Agrochemistry and Food Technology, Consejo Superior de Investigaciones Científicas (IATA-CSI C), Agustí Escardino 7, 46980 Paterna, València, Spain.
authors.forename: Francesc A
authors.initials: FA
authors.lastname: Esteve-Turrillas
authors.forename: Josep V
authors.initials: JV
authors.lastname: Mercader
authors.forename: Consuelo
authors.initials: C
authors.lastname: Agulló
authors.forename: Antonio
authors.initials: A
authors.lastname: Abad-Somovilla
authors.forename: Antonio
authors.initials: A
authors.lastname: Abad-Fuentes
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology*; D000915:Antibody Affinity; D005260:Female; D006241:Haptens / Q000737:chemistry / Q000276:immunology*; D007118:Immunoassay / Q000379:methods*; D011743:Pyrimidines / Q000032:analysis / Q000276:immunology*; D011817:Rabbits
chemical_list: D000906:Antibodies; D006241:Haptens; D011743:Pyrimidines; C104083:mepanipyrim