title: Novel multistep BRET-FRET energy transfer using nanoconjugates of firefly proteins, quantum dots, and red fluorescent proteins.
abstract: Sequential bioluminescence resonance energy transfer (BRET) and fluorescence resonance energy transfer (FRET) from firefly luciferase to red fluorescent proteins using quantum dot or rod acceptor/donor linkers is described. The effect of morphology and tuned optical properties on the efficiency of this unique BRET-FRET system was evaluated.
authors.affiliation: Department of Chemistry, Syracuse University, Syracuse, New York 13244, USA.
authors.forename: Rabeka
authors.initials: R
authors.lastname: Alam
authors.forename: Joshua
authors.initials: J
authors.lastname: Zylstra
authors.forename: Danielle M
authors.initials: DM
authors.lastname: Fontaine
authors.forename: Bruce R
authors.initials: BR
authors.lastname: Branchini
authors.forename: Mathew M
authors.initials: MM
authors.lastname: Maye
mesh_terms: D000818:Animals; D048888:Fireflies / Q000201:enzymology; D031541:Fluorescence Resonance Energy Transfer; D049409:Luciferases, Firefly / Q000737:chemistry*; D008164:Luminescent Proteins / Q000737:chemistry*; D045663:Quantum Dots; D000097573:Red Fluorescent Protein
chemical_list: D049409:Luciferases, Firefly; D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein