title: Oligomerization of TopBP1 is necessary for the localization of TopBP1 to mitotic centrosomes.
abstract: Human TopBP1 is involved in the DNA damage checkpoint response, chromosome replication, and other functions of cell cycle control. The C-terminal region of TopBP1 (TbpCtr: amino acid residues 1222-1522) is involved in the localization of TopBP1 to the centrosomes during mitosis. Here, we showed that the amino acid residues 741-885 of TopBP1, in addition to TbpCtr, are necessary for the centrosomal localization of TopBP1. Whereas oligomeric tags fused to TbpCtr localized to mitotic centrosomes, monomeric tags fused to TbpCtr did not. Insertion of the amino acid residues 741-885 into the monomeric tag fused to TbpCtr allowed the protein to localize to the mitotic centrosome. These results suggest that the amino acid residues 741-885 are necessary for oligomerization of TopBP1 for centrosomal localization.
authors.affiliation: Department of Biological Sciences, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Sung Woong
authors.initials: SW
authors.lastname: Bang
authors.forename: Gwang Su
authors.initials: GS
authors.lastname: Kim
authors.forename: Deog Su
authors.initials: DS
authors.lastname: Hwang
mesh_terms: D002352:Carrier Proteins / Q000378:metabolism*; D002453:Cell Cycle; D018385:Centrosome / Q000648:ultrastructure; D002875:Chromosomes / Q000648:ultrastructure*; D004249:DNA Damage; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005786:Gene Expression Regulation; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008938:Mitosis; D009687:Nuclear Proteins / Q000378:metabolism*; D010766:Phosphorylation; D015662:Trimethoprim, Sulfamethoxazole Drug Combination / Q000737:chemistry
chemical_list: D002352:Carrier Proteins; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; C110513:TOPBP1 protein, human; D049452:Green Fluorescent Proteins; D015662:Trimethoprim, Sulfamethoxazole Drug Combination