title: Enhanced morphinan alkaloid production in hairy root cultures of Papaver bracteatum by over-expression of salutaridinol 7-o-acetyltransferase gene via Agrobacterium rhizogenes mediated transformation.
abstract: Papaver bracteatum is an important medicinal plant valued for its high content of thebaine and an alternative to P. somniferum for benzylisoquinoline alkaloid production. Salutaridinol 7-o-acetyltransferase (SalAT) is a key gene in morphinan alkaloids biosynthesis pathway. Over expression of SalAT gene was used for metabolic engineering in P. bracteatum hairy root cultures. Transcript level of the salutaridinol 7-o-acetyltransferase gene in transgenic hairy root lines increased up to 154 and 128 % in comparison with hairy roots without SalAT over expression and wild type roots, respectively. High performance liquid chromatography analysis showed that the transgenic hairy roots relatively improved levels of thebaine (1.28 % dry weight), codeine (0.02 % dry weight) and morphine (0.03 % dry weight) compared to those hairy roots without SalAT over expression. This suggests that P. bracteatum hairy roots expressing the SalAT gene could be potentially used for the production of valuable morphinan alkaloids.
authors.affiliation: National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Sharafi
authors.forename: Haleh
authors.initials: H
authors.lastname: Hashemi Sohi
authors.forename: Amir
authors.initials: A
authors.lastname: Mousavi
authors.forename: Pejman
authors.initials: P
authors.lastname: Azadi
authors.forename: Bahareh
authors.initials: B
authors.lastname: Dehsara
authors.forename: Bahman
authors.initials: B
authors.lastname: Hosseini Khalifani
mesh_terms: D000123:Acetyltransferases / Q000235:genetics* / Q000378:metabolism*; D060054:Agrobacterium / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D060847:Metabolic Engineering; D009019:Morphinans / Q000378:metabolism*; D010207:Papaver / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D030821:Plants, Genetically Modified; D014170:Transformation, Genetic
chemical_list: D009019:Morphinans; D010940:Plant Proteins; D000123:Acetyltransferases; C097501:acetyl coenzyme A-salutaridinol-7-O-acetyltransferase