title: Limited intra-genetic diversity in Dientamoeba fragilis housekeeping genes.
abstract: Dientamoeba fragilis is a common intestinal parasite of unsettled clinical significance. Differences in clinical outcome of parasitic infections may reflect parasite genetic diversity, and so tools to study intra-genetic diversity that could potentially reflect differences in clinical phenotypes are warranted. Here, we show that genetic analysis of three D. fragilis housekeeping genes enables clear distinction between the two known genotypes, but that integration of housekeeping genes in multi-locus sequencing tools for D. fragilis may have limited epidemiological and clinical value due to no further added genetic resolution.
authors.affiliation: Laboratory of Parasitology, Department of Microbiology and Infection Control, Statens Serum Institut, Artillerivej 5, DK-2300 Copenhagen S, Denmark. run@ssi.dk
authors.forename: Christen Rune
authors.initials: CR
authors.lastname: Stensvold
authors.forename: C Graham
authors.initials: CG
authors.lastname: Clark
authors.forename: Dennis
authors.initials: D
authors.lastname: Röser
mesh_terms: D000293:Adolescent; D000328:Adult; D002648:Child; D016000:Cluster Analysis; D016054:DNA, Protozoan / Q000235:genetics; D004029:Dientamoeba / Q000145:classification / Q000235:genetics*; D004030:Dientamoebiasis / Q000469:parasitology*; D005243:Feces / Q000469:parasitology; D017125:Genes, Protozoan; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D017720:Molecular Epidemiology; D058885:Multilocus Sequence Typing; D010802:Phylogeny
chemical_list: D016054:DNA, Protozoan
keywords: Actin; Dientamoeba; Elongation factor; Genetic diversity; Molecular epidemiology; Multilocus sequence typing