title: Growth inhibition and apoptosis induction of human umbilical vein endothelial cells by apogossypolone.
abstract: OBJECTIVE
  Prostate cancer is one of the most common malignant tumors in the male reproductive system, which causes the second most cancer deaths of males, and control of angiogenesis in prostate lesions is of obvious importance. This study assessed the effect of apogossypolone (ApoG2) on proliferation and apoptosis of human umbilical vein endothelial cells (HUVECs).
  
  
  METHODS
  HUVECs were treated with different concentrations of ApoG2. The survival rate of HUVECs were determined by MTT assay. Utrastructural changes of HUVECs were assessed with transmission electron microscopy. Apoptosis in HUVECs was analyzed by flow cytometry and cell migration by Boyden chamber assay. Matrigel assays were used to quantify the development of tube-like networks.
  
  
  RESULTS
  ApoG2 significantly inhibited HUVEC growth even at 24 h (P<0.05). The inhibitory effect of ApoG2 is more obvious as the concentration and the culture time increased (P<0.05). These results indicate that ApoG2 inhibits the proliferation of HUVECs in a time- and concentration-dependent manner with increase of the apoptosis rate. Besides, ApoG2 reduced the formation of total pseudotubule length and network branches of HUVECs.
  
  
  CONCLUSIONS
  The results suggest that ApoG2 inhibits angiogenesis of HUVECs by growth inhibition and apoptosis induction.
authors.affiliation: School of Life Sciences and Technology, Xidian University, China. zhangxq@fmmu.edu.cn
authors.forename: Yong-Hua
authors.initials: YH
authors.lastname: Zhan
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Huang
authors.forename: Xing-Bin
authors.initials: XB
authors.lastname: Hu
authors.forename: Qun-Xing
authors.initials: QX
authors.lastname: An
authors.forename: Zhi-Xin
authors.initials: ZX
authors.lastname: Liu
authors.forename: Xian-Qing
authors.initials: XQ
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D004338:Drug Combinations; D005434:Flow Cytometry; D006072:Gossypol / Q000031:analogs & derivatives* / Q000494:pharmacology; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D007797:Laminin / Q000378:metabolism; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D011509:Proteoglycans / Q000378:metabolism
chemical_list: D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C534401:apogossypolone; C059485:matrigel; D003094:Collagen; D006072:Gossypol