title: Growth cone collapse assay.
abstract: Growth cone collapse is an easy and efficient test for detecting and characterizing axon guidance activities secreted or expressed by cells. It can also be used to dissect signaling pathways by axon growth inhibitors and to isolate therapeutic compounds that promote axon regeneration. Here, we describe a growth cone collapse assay protocol used to study signal transduction mechanisms of the repulsive axon guidance molecule ephrin-A5 in hippocampal neurons.
authors.affiliation: Department of Chemical Biology, Susan Lehman Cullman Laboratory for Cancer Research, Ernest Mario School of Pharmacy, Rutgers University, Piscataway, NJ, USA.
authors.forename: Xin
authors.initials: X
authors.lastname: Yue
authors.forename: Alexander I
authors.initials: AI
authors.lastname: Son
authors.forename: Renping
authors.initials: R
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods*; D002478:Cells, Cultured; D003432:Cross-Linking Reagents / Q000494:pharmacology; D036342:Ephrins / Q000378:metabolism; D020439:Growth Cones / Q000378:metabolism*; D006624:Hippocampus / Q000166:cytology; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling; D016707:Tissue Fixation
chemical_list: D003432:Cross-Linking Reagents; D036342:Ephrins