title: Tumour regression induced by co-administration of MIP-3α and CpG in an experimental model of colon carcinoma.
abstract: CCL20/macrophage inflammatory protein-3α (MIP-3α) represents one of the potent chemoattractive proteins for dendritic cells (DCs). Herein, we investigated whether in vivo genetic modification of tumour cells aimed at intratumoural production of MIP-3α might lead to accumulation of DCs in tumour tissue. Mice injected with CT26, received recombinant adenovirus (Ad) vectors (AdMIP-3α) expressing MIP-3α protein. This was complemented by injections of CpG. Interestingly, MIP-3α gene therapy combined with CpG injections resulted in specific cytotoxicity. This was associated with significant suppression of tumour growth rate. These findings demonstrate the potential of strategies that utilize in vivo overexpression of chemokines.
authors.affiliation: Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: S
authors.initials: S
authors.lastname: Arab
authors.forename: M
authors.initials: M
authors.lastname: Mojarrad
authors.forename: M
authors.initials: M
authors.lastname: Motamedi
authors.forename: R
authors.initials: R
authors.lastname: Mirzaei
authors.forename: M H
authors.initials: MH
authors.lastname: Modarressi
authors.forename: J
authors.initials: J
authors.lastname: Hadjati
mesh_terms: D000818:Animals; D054418:Chemokine CCL20 / Q000235:genetics* / Q000502:physiology; D002630:Chemotactic Factors / Q000502:physiology; D003110:Colonic Neoplasms / Q000473:pathology / Q000628:therapy*; D003602:Cytotoxicity, Immunologic; D004195:Disease Models, Animal; D005260:Female; D015316:Genetic Therapy; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D009838:Oligodeoxyribonucleotides / Q000627:therapeutic use*
chemical_list: C408982:CPG-oligonucleotide; D054418:Chemokine CCL20; D002630:Chemotactic Factors; D009838:Oligodeoxyribonucleotides