title: Molecularly imprinted poly(hydroxyethyl methacrylate) based cryogel for albumin depletion from human serum.
abstract: Macroporous cryogels imprinted with human serum albumin (HSA) have been prepared by copolymerization of 2-hydroxyethyl methacrylate with a functional co-monomer of N-methacryloyl-L-phenylalanine. The cryogels were used for the depletion of HSA from human serum. HSA-imprinted cryogels were prepared with gel fraction yields up to 90%, and their chemical structure, morphology and porosity were characterized by FTIR-spectroscopy, scanning electron microscopy, swelling studies and flow dynamics. Selective binding experiments were performed in the presence of competitive proteins like human transferrin and myoglobin. Albumin-imprinted cryogel column was optimized for fast protein liquid chromatography. Sodium-dodecyl sulfate polyacrylamide gel electrophoresis was used to show the efficiency of albumin depletion.
authors.affiliation: Biochemistry Division, Department of Chemistry, Hacettepe University, Ankara, Turkey.
authors.forename: Muge
authors.initials: M
authors.lastname: Andac
authors.forename: Igor Yu
authors.initials: IY
authors.lastname: Galaev
authors.forename: Adil
authors.initials: A
authors.lastname: Denizli
mesh_terms: D000327:Adsorption; D059985:Cryogels / Q000737:chemistry*; D006801:Humans; D054802:Molecular Imprinting; D015394:Molecular Structure; D010316:Particle Size; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry*; D016062:Porosity; D044967:Serum / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry / Q000302:isolation & purification*; D013499:Surface Properties
chemical_list: D059985:Cryogels; D012709:Serum Albumin; D011102:Polyhydroxyethyl Methacrylate