title: [Assessment of the effect of influent NH+4-N concentration on the abundance and community structure of functional bacteria in CANON process].
abstract: To investigate macroscopic performance and microcosmic ecosystem of CANON process in low ammonium concentration at room temprature, a steady-operation CANON reactor was achieved in different ammonium concentrations by adjusting aeration and hydraulic retention time (HRT), and the effect of ammonium concentration on the abundance and community structure of functional bacteria was analyzed using PCR-DGGE and real-time PCR. The results indicated a high TN removal loading of over 1.0 g.(L.d)-1 in relatively high ammonium which was significantly reduced with the ammonium concentration of 100 mg.L-1. The community structure of ammonium oxidizing bacteria (AOB) changed sharply with the decrease of ammonium concentraion, which was not the same as anaerobic ammonium oxidizing bacteria (ANAMMOX bacteria). Besides, the abundance of the two functional groups of bacteria reduced while the population of nitrite oxidizing bacteria (NOB) rose with the decrease of ammonium, which might be the main reason for the reduction of nitrogen removal efficiency. Consequently, some strategies are needed to reduce the loss of AOB and ANAMMOX bacteria and inhibit the growth of NOB so as to maintain the nitrogen removal performance in low ammonium concentration.
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China. hittaoliu@gmail.com
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.forename: Dong
authors.initials: D
authors.lastname: Li
authors.forename: Hui-Ping
authors.initials: HP
authors.lastname: Zeng
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Chang
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000641:Ammonia / Q000032:analysis / Q000302:isolation & purification* / Q000378:metabolism; D052818:Autotrophic Processes; D001419:Bacteria / Q000145:classification / Q000254:growth & development* / Q000378:metabolism; D001673:Biodegradation, Environmental; D044822:Biodiversity; D019149:Bioreactors / Q000382:microbiology; D009573:Nitrites / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism; D009584:Nitrogen / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism; D010084:Oxidation-Reduction; D014865:Waste Disposal, Fluid / Q000379:methods*
chemical_list: D009573:Nitrites; D000641:Ammonia; D009584:Nitrogen