title: [Experimental studies on pharmacokinetics of three components in Buyanghuanwu injection on base of total quantum statistical moment].
abstract: OBJECTIVE
  To verify established the total quantum statistic moments model with astragaloside IV, paeoniflorin, tetramethylpyrazine in Buyanghuanwu injection, in order to establish a pharmacokinetic experimental method with multi-component traditional Chinese medicine (TCM) compound system.
  
  
  METHODS
  The RP-HPLC was adopted, with the chromatographic column of C18, 4.6 mm x 250 mm, 5 microm. As for astragaloside IV, the ELSD detector was adopted with acetonitrile-water (35: 65) as the mobile phase at 1 mL x min(-1); the pressure of column was (15.0 +/- 0.408) MPa, the column temperature was 30 degrees C. Regarding paeoniflorin and tetramethylpyrazine, the detection of wavelengths was 254 nm, with acetonitrile-water (35:65) as the mobile phase at 1 mL x min(-1), the column pressure of (15.17 +/- 0.41) MPa. The pharmacokinetic parameters for single component were dealt with DAS and the total quantum statistical moment (TQSM) parameters were calculated using formulations.
  
  
  RESULTS
  All of the three components followed the two compartmental pharkacokinetic model (P < 0.01) in rats. Compared with the superimposed total concentration, each single component showed difference in parameters up to 10 000 times at most, whereas the RSD of TQSM parameters was 3.510%. The TQSM pharmacokinetic parameters of the three components in Buyanghuanwu injection showed that AUC(t), MRT(t), VRT(t), CL(t), V(t), were (119.8 +/- 27.20) g x min x L(-1), (210.0 +/- 54.49) min, (5.608 +/- 2.723) x 10(4) min2, (0.319 6 +/- 0.068 8) mL x min(-1) x kg(-1) and (64.12 +/- 8.243) mL x kg(-1), respectively, suggesting that the half-life time for the three components were (145.5 +/- 37.76) min and 95% of them were metabolized within 0-674. 2 min.
  
  
  CONCLUSIONS
  The TQSM can be used to study pharmacokinetic parameters of multi-component TCM compound, because the method can characterize the pharmacokinetic regularity of quantum-time change in a multi-component system.
authors.affiliation: Department of Pharmaceutics, Hunan University of Traditional Chinese Medicine, Changsha 410208, China. pharmsharking@tom.com
authors.forename: Fu-Yuan
authors.initials: FY
authors.lastname: He
authors.forename: Kai-Wen
authors.initials: KW
authors.lastname: Deng
authors.forename: Wen-Long
authors.initials: WL
authors.lastname: Liu
authors.forename: Ji-Lian
authors.initials: JL
authors.lastname: Shi
authors.forename: Yong
authors.initials: Y
authors.lastname: Wu
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Qing-Ping
authors.initials: QP
authors.lastname: He
authors.forename: Bo
authors.initials: B
authors.lastname: Li
mesh_terms: D000818:Animals; D001565:Benzoates / Q000493:pharmacokinetics*; D001952:Bridged-Ring Compounds / Q000493:pharmacokinetics*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics*; D005960:Glucosides / Q000493:pharmacokinetics*; D008297:Male; D008516:Medicine, Chinese Traditional; D015233:Models, Statistical; D039821:Monoterpenes; D011719:Pyrazines / Q000493:pharmacokinetics*; D051381:Rats; D012503:Saponins / Q000493:pharmacokinetics*; D014315:Triterpenes / Q000493:pharmacokinetics*; D006027:Glycosides
chemical_list: D001565:Benzoates; D001952:Bridged-Ring Compounds; D004365:Drugs, Chinese Herbal; D005960:Glucosides; D039821:Monoterpenes; D011719:Pyrazines; D012503:Saponins; D014315:Triterpenes; C460102:buyang huanwu; C015423:peoniflorin; D006027:Glycosides; C052064:astragaloside A; C017953:tetramethylpyrazine