title: Prdm14 promotes germline fate and naive pluripotency by repressing FGF signalling and DNA methylation.
abstract: Primordial germ cells (PGCs) and somatic cells originate from postimplantation epiblast cells in mice. As pluripotency is lost upon differentiation of somatic lineages, a naive epigenome and the pluripotency network are re-established during PGC development. Here we demonstrate that Prdm14 contributes not only to PGC specification, but also to naive pluripotency in embryonic stem (ES) cells by repressing the DNA methylation machinery and fibroblast growth factor (FGF) signalling. This indicates a critical role for Prdm14 in programming PGCs and promoting pluripotency in ES cells.
authors.affiliation: Wellcome Trust/Cancer Research UK Gurdon Institute, Department of Physiology, Development and Neuroscience, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK.
authors.forename: Nils
authors.initials: N
authors.lastname: Grabole
authors.forename: Julia
authors.initials: J
authors.lastname: Tischler
authors.forename: Jamie A
authors.initials: JA
authors.lastname: Hackett
authors.forename: Shinseog
authors.initials: S
authors.lastname: Kim
authors.forename: Fuchou
authors.initials: F
authors.lastname: Tang
authors.forename: Harry G
authors.initials: HG
authors.lastname: Leitch
authors.forename: Erna
authors.initials: E
authors.lastname: Magnúsdóttir
authors.forename: M Azim
authors.initials: MA
authors.lastname: Surani
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D019175:DNA Methylation; D004268:DNA-Binding Proteins; D053595:Embryonic Stem Cells / Q000166:cytology / Q000378:metabolism*; D005346:Fibroblast Growth Factors / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D005854:Germ Cells / Q000166:cytology / Q000378:metabolism*; D005855:Germ Layers / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000378:metabolism*; D016601:RNA-Binding Proteins; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; C531066:Prdm14 protein, mouse; D016601:RNA-Binding Proteins; D014157:Transcription Factors; D005346:Fibroblast Growth Factors; D020928:Mitogen-Activated Protein Kinases