title: Plants from northeast Mexico with anti-HSV activity.
abstract: Based on chemotaxonomic and ethno-pharmacological criteria, three Mexican plants (Jatropha dioica, Salvia texana and S. ballotaeflora) were studied for in vitro activity against HSV-1 and HSV-2. Hydro-methanolic extracts were initially evaluated for their toxicity to Vero cells. Both Salvia species displayed cytotoxicity at the lowest dose (125 microg/mL). The J. dioica extract showed only negligible cytotoxicity (CC50 644 microg/mL). Its anti-HSV activity was evaluated using the plaque reduction assay with HSV-1 and HSV-2 (from clinical isolates) infected Vero cells. The hydro-methanolic extract of J. dioica showed IC50s of 280 and 370 microg/mL against HSV-1 and HSV-2, respectively. The n-hexane liquid-liquid partition of J. dioica extract contained the majority of the active principle(s) with IC50 values of 300 and 270 microg/mL for HSV-1 and HSV-2, respectively. Bioassay-guided isolation led to the known diterpene, riolozatrione.
authors.affiliation: Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey Nuevo León, México.
authors.forename: David
authors.initials: D
authors.lastname: Silva-Mares
authors.forename: Ernesto
authors.initials: E
authors.lastname: Torres-López
authors.forename: Ana M
authors.initials: AM
authors.lastname: Rivas-Estilla
authors.forename: Paula
authors.initials: P
authors.lastname: Cordero-Pérez
authors.forename: Noemí
authors.initials: N
authors.lastname: Waksman-Minsky
authors.forename: Verónica M
authors.initials: VM
authors.lastname: Rivas-Galindo
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology; D018259:Herpesvirus 1, Human / Q000187:drug effects*; D018258:Herpesvirus 2, Human / Q000187:drug effects*; D006801:Humans; D031286:Jatropha / Q000737:chemistry*; D008800:Mexico; D010936:Plant Extracts / Q000737:chemistry* / Q000494:pharmacology*; D027544:Salvia / Q000737:chemistry*; D014709:Vero Cells
chemical_list: D000998:Antiviral Agents; D010936:Plant Extracts