title: Geographic diversity of Helicobacter pylori in cadavers: forensic estimation of geographical origin.
abstract: A method for determining the geographical origin of unidentified cadavers by determining the genotype of Helicobacter pylori, which is latent in one-half of the world's population, was developed. In the first stage, DNA was extracted from samplings at 5 points in the gastric mucosa of 177 individuals randomly selected from cadavers undergoing medico-legal autopsy. 16S-rDNA of H. pylori DNA was detected by polymerase chain reaction (PCR) in 101 cadavers (57.0%); by sex, 74 of 123 (60.1%) males and 28 of 54 (46.4%) females were positive. There were no significant differences in H. pylori detection rate among the 5 sampling points of the gastric mucosa, cause of death, or age. In the second stage, amplified fragments of H. pylori vacA regions s and m from 17 individuals with the following ethnic backgrounds were sequenced: Japanese, 10; Chinese, 2; South Korean, 1; Taiwanese, 1; Thai, 1; Afghan, 1; and Filipino, 1. A phylogenetic tree constructed with these and 28 previously reported H. pylori strain sequences revealed 3 major gene clusters consisting of East Asian type I (Japanese, South Korean and Chinese), Western type II, and Southeast Asia type III. The Taiwanese and Filipino samples deviated from the clusters type III to which they typically belong. The ultimate aim of the present study was to develop a more accurate method of determining of geographic origin of unidentified cadavers through the combination of the present method with other, virus-based methods H. pylori DNA was detected from over half of the cadavers tested and vacA genotypes showed specificity to geographical origin. Therefore, these results suggest that the H. pylori genome provides valuable additional information for tracing the geographical origin of unidentified cadavers.
authors.affiliation: Department of Legal Medicine, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan. nagasawa.s@graduate.chiba-u.jp
authors.forename: Sayaka
authors.initials: S
authors.lastname: Nagasawa
authors.forename: Hisako
authors.initials: H
authors.lastname: Motani-Saitoh
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Inoue
authors.forename: Hirotaro
authors.initials: H
authors.lastname: Iwase
mesh_terms: D002102:Cadaver; D004269:DNA, Bacterial / Q000235:genetics*; D005006:Ethnicity; D005260:Female; D049429:Forensic Pathology; D005753:Gastric Mucosa / Q000382:microbiology*; D016680:Genome, Bacterial; D005838:Genotype; D016480:Helicobacter pylori / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D044469:Racial Groups; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S