title: Analysis of key genes and pathways associated with colorectal cancer with microarray technology.
abstract: OBJECTIVE
  Microarray data were analyzed to explore key genes and their functions in progression of colorectal cancer (CRC).
  
  
  METHODS
  Two microarray data sets were downloaded from Gene Expression Omnibus (GEO) database and differentially expressed genes (DEGs) were identified using corresponding packages of R. Functional enrichment analysis was performed with DAVID tools to uncover their biological functions.
  
  
  RESULTS
  631 and 590 DEGs were obtained from the two data sets, respectively. A total of 32 common DEGs were then screened out with the rank product method. The significantly enriched GO terms included inflammatory response, response to wounding and response to drugs. Two interleukin-related domains were revealed in the domain analysis. KEGG pathway enrichment analysis showed that the PPAR signaling pathway and the renin-angiotensin system were enriched in the DEGs.
  
  
  CONCLUSIONS
  Our study to systemically characterize gene expression changes in CRC with microarray technology revealed changes in a range of key genes, pathways and function modules. Their utility in diagnosis and treatment now require exploration.
authors.affiliation: Department of General Surgery, The Third People's Hospital of Chengdu, The Second Clinical College Affiliated to Chongqing Medical University, Chengdu, Sichuan, China.
authors.forename: Yan-Jun
authors.initials: YJ
authors.lastname: Liu
authors.forename: Shu
authors.initials: S
authors.lastname: Zhang
authors.forename: Kang
authors.initials: K
authors.lastname: Hou
authors.forename: Yun-Tao
authors.initials: YT
authors.lastname: Li
authors.forename: Zhan
authors.initials: Z
authors.lastname: Liu
authors.forename: Hai-Liang
authors.initials: HL
authors.lastname: Ren
authors.forename: Dan
authors.initials: D
authors.lastname: Luo
authors.forename: Shi-Hong
authors.initials: SH
authors.lastname: Li
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D011379:Prognosis; D012084:Renin-Angiotensin System; D015398:Signal Transduction
chemical_list: D014408:Biomarkers, Tumor