title: Improved neural progenitor cell proliferation and differentiation on poly(lactide-co-glycolide) scaffolds coated with elastin-like polypeptide.
abstract: Poly(lactide-co-glycolide) (PLGA) and elastin-like polypeptide (ELP) have been widely used as a biodegradable scaffold and thermoresponsive matrix, respectively. However, little attention has focused on the combinatorial use of these biomaterials for tissue engineering applications. An ELP matrix TGPG[VGRGD(VGVPG)6]20WPC (referred to as REP) contains multiple Arg-Gly-Asp motifs. This study fabricated porous PLGA scaffolds coated with various concentration of matrix via thermally induced phase transition to improve adhesion-mediated proliferation and differentiation of neural progenitor cells. Matrix-coated scaffolds were characterized by FTIR, SEM, and hematoxylin and eosin staining with respect to coating efficiency, porosity, and pore size and shape. On the matrix-coated scaffolds, cells grew as a single cell or associated each other to form a multicellular layer or cluster. In biological evaluations, cell adhesion and proliferation were significantly promoted in a matrix concentration-dependent manner. More importantly, in combination with retinoic acid, the differentiation of progenitor cells into neuronal and astroglial lineages was highly stimulated in the cells cultured on matrix-coated scaffolds than on untreated controls. Taken together, our results indicated that the REP matrix-functionalized PLGA scaffolds are suitable for improving neuronal cell functions, and thus applicable for neural tissue engineering.
authors.affiliation: Laboratory of Biochemistry and Cellular Engineering, Division of NanoBio Technology, Daegu Gyeongbuk Institute of Science and Technology, Daegu, 711-873, South Korea.
authors.forename: Seong-Kyoon
authors.initials: SK
authors.lastname: Choi
authors.forename: Jin-Kyu
authors.initials: JK
authors.lastname: Park
authors.forename: Kyeong-Min
authors.initials: KM
authors.lastname: Lee
authors.forename: Soo-Keun
authors.initials: SK
authors.lastname: Lee
authors.forename: Won Bae
authors.initials: WB
authors.lastname: Jeon
mesh_terms: D020816:Amino Acid Motifs; D001253:Astrocytes / Q000166:cytology; D015415:Biomarkers / Q000378:metabolism; D002448:Cell Adhesion; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D004549:Elastin / Q000737:chemistry*; D006801:Humans; D058953:Neural Stem Cells / Q000166:cytology*; D010455:Peptides / Q000737:chemistry; D011098:Polyglactin 910 / Q000737:chemistry*; D016062:Porosity; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry*; D014212:Tretinoin / Q000737:chemistry
chemical_list: D015415:Biomarkers; D010455:Peptides; D011098:Polyglactin 910; D014212:Tretinoin; D004549:Elastin
keywords: PLGA scaffolds; extracellular matrix; stem/progenitor cells; surface modification; tissue engineering