title: Comprehensive proteomic analysis of Trypanosoma cruzi epimastigote cell surface proteins by two complementary methods.
abstract: Trypanosoma cruzi is a protozoan that causes Chagas' disease, a neglected infectious illness that affects millions of people, mostly in Latin America. Here, the cell surface subproteome of the T. cruzi epimastigote life form was characterized. In order to prepare samples enriched in epimastigote plasma membrane protein, two distinct methodologies were optimized and evaluated. The first methodology was based on cell surface trypsinization (Shave) of intact living cells while the second approach used biotinylation of cell surface proteins followed by streptavidin affinity chromatography isolation of the labeled proteins. Both T. cruzi subproteomes were analyzed by LC-MS/MS. The results showed that the methodologies offered comprehensive and complementary information about the parasite's plasma membrane subproteome.
authors.affiliation: Department of Cell Biology, Institute of Biology, University of Brasilia, Brasilia, CEP 70910-900, Brazil.
authors.forename: Rayner M L
authors.initials: RM
authors.lastname: Queiroz
authors.forename: Sébastien
authors.initials: S
authors.lastname: Charneau
authors.forename: Flávia N
authors.initials: FN
authors.lastname: Motta
authors.forename: Jaime M
authors.initials: JM
authors.lastname: Santana
authors.forename: Peter
authors.initials: P
authors.lastname: Roepstorff
authors.forename: Carlos A O
authors.initials: CA
authors.lastname: Ricart
mesh_terms: D019921:Biotinylation; D002462:Cell Membrane / Q000378:metabolism; D014355:Chagas Disease / Q000175:diagnosis* / Q000473:pathology; D006801:Humans; D008565:Membrane Proteins / Q000302:isolation & purification* / Q000378:metabolism; D020543:Proteome / Q000032:analysis*; D053719:Tandem Mass Spectrometry; D014349:Trypanosoma cruzi / Q000378:metabolism* / Q000472:pathogenicity; D014357:Trypsin / Q000737:chemistry
chemical_list: D008565:Membrane Proteins; D020543:Proteome; D014357:Trypsin