title: MicroRNA profiling in chemoresistant and chemosensitive acute myeloid leukemia.
abstract: MicroRNA (miRNA) deregulation is associated with progression and treatment outcome in various types of cancers. To identify miRNAs related to therapeutic response, we applied an miRNA microarray followed by PCR verification of 33 available diagnostic bone marrow core biopsies from 33 acute myeloid leukemia patients including 15 chemoresistant and 18 chemosensitive patients. We found 3 significantly upregulated miRNAs, miR-363, miR-532-5p and miR-342-3p, related to therapeutic response (q < 0.05). Further validation of miR-532-5p and miR-363 expression by quantitative RT-PCR confirmed microarray analysis results. Genes targeted by miR-363 include RGS17 and HIPK3, both reported to be associated with drug response.
authors.affiliation: Department of Pathology, Haartman Institute and HUSLAB, University of Helsinki and Helsinki University Central Hospital, Helsinki, Finland.
authors.forename: N
authors.initials: N
authors.lastname: Mosakhani
authors.forename: R
authors.initials: R
authors.lastname: Räty
authors.forename: A
authors.initials: A
authors.lastname: Tyybäkinoja
authors.forename: M-L
authors.initials: ML
authors.lastname: Karjalainen-Lindsberg
authors.forename: E
authors.initials: E
authors.lastname: Elonen
authors.forename: S
authors.initials: S
authors.lastname: Knuutila
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001854:Bone Marrow Cells; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D015470:Leukemia, Myeloid, Acute / Q000188:drug therapy* / Q000235:genetics; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D020710:RGS Proteins / Q000235:genetics / Q000378:metabolism; D055815:Young Adult
chemical_list: D047908:Intracellular Signaling Peptides and Proteins; C546176:MIRN342 microRNA, human; C574861:MIRN363 microRNA, human; C585583:MIRN532 microRNA, human; D035683:MicroRNAs; D020710:RGS Proteins; C550555:RGS17 protein, human; C109102:HIPK3 protein, human; D017346:Protein Serine-Threonine Kinases