title: Antimicrobial susceptibility pattern of extended spectrum beta-lactamase producing gram-negative bacteria isolated from wound and urine in a tertiary care hospital, Dhaka City, Bangladesh.
abstract: From a total of 320 bacterial samples from wound swab and urine 169 (53%) gram-negative bacteria were isolated, of which 42 (25%) extended-spectrum beta-lactamase (ESBL) producers were detected by double-disk synergy test. ESBL producers were significantly more resistant against amoxiclav, Co-trimoxazole, ciprofloxacin, amikacin and gentamicin than non-ESBL producers. Among the 42 ESBL producers, 76% were positive for blaCTX-M and 43% were positive for blaOXA, with blaCTX-M predominantly (97%) observed in E. coli and blaOXA predominantly (80%) in Pseudomonas spp. Class 1 integron was found in 75% of blaCTX-M positive and 56% of blaOXA positive strains. Combinations of ESBL genes and class 1 integron were observed in 29 (69%) of the ESBL producers. The findings of this study infer that CTX-M and OXA producers are emerging in Bangladesh and we report the presence of blaOXA for the first time in Bangladesh.
authors.affiliation: Department of Microbiology, Dhaka, Medical College, Dhaka, Bangladesh. refath_farzana@yahoo.com
authors.forename: Refath
authors.initials: R
authors.lastname: Farzana
authors.forename: S M
authors.initials: SM
authors.lastname: Shamsuzzaman
authors.forename: K Z
authors.initials: KZ
authors.lastname: Mamun
authors.forename: Paul
authors.initials: P
authors.lastname: Shears
mesh_terms: D000595:Amino Acid Sequence; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001431:Bacteriological Techniques; D001459:Bangladesh / Q000453:epidemiology; D005798:Genes, Bacterial; D006090:Gram-Negative Bacteria / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D032023:Integrons; D062606:Tertiary Care Centers; D016482:Urinalysis; D014947:Wounds and Injuries / Q000382:microbiology*; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D000900:Anti-Bacterial Agents; D001618:beta-Lactamases