title: Analyzing plant signaling phospholipids through 32Pi-labeling and TLC.
abstract: Lipidomic analyses through LC-, GC-, and ESI-MS/MS can detect numerous lipid species based on headgroup and fatty acid compositions but usually miss the minor phospholipids involved in cell signaling because of their low chemical abundancy. Due to their high turnover, these signaling lipids are, however, readily picked up by labeling plant material with (32)P-orthophosphate and subsequent analysis of the lipid extracts by thin layer chromatography. Here, protocols are described for suspension-cultured tobacco BY-2 cells, young Arabidopsis seedlings, Vicia faba roots, and Arabidopsis leaf disks, which can easily be modified for other plant species and tissues.
authors.affiliation: Swammerdam Institute for Life Sciences, Section Plant Physiology, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Teun
authors.initials: T
authors.lastname: Munnik
authors.forename: Xavier
authors.initials: X
authors.lastname: Zarza
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000378:metabolism*; D002855:Chromatography, Thin Layer / Q000379:methods*; D007553:Isotope Labeling; D010743:Phospholipids / Q000302:isolation & purification / Q000378:metabolism*; D010761:Phosphorus Radioisotopes; D018515:Plant Leaves / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D010944:Plants / Q000378:metabolism*; D036226:Seedlings / Q000378:metabolism; D015398:Signal Transduction; D013535:Suspensions; D014026:Nicotiana / Q000166:cytology / Q000378:metabolism; D031306:Vicia / Q000166:cytology / Q000378:metabolism
chemical_list: D010743:Phospholipids; D010761:Phosphorus Radioisotopes; D013535:Suspensions