title: Genetic and functional characterization of culturable plant-beneficial actinobacteria associated with yam rhizosphere.
abstract: Actinobacteria were isolated from the rhizosphere of yam plants from agricultural fields from Yeoju, South Korea and analyzed for their genetic and plant-beneficial functional diversity. A total of 29 highly occurring actinobacterial isolates from the yam rhizosphere were screened for various plant-beneficial traits such as antimicrobial activity on fungi and bacteria; biocontrol traits such as production of siderophore, protease, chitinase, endo-cellulase, and β-glucanase. The isolates were also screened for plant growth-promoting (PGP) traits such as auxin production, phosphate solubilization, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity, and in vitro Arabidopsis growth promotion. 16S rDNA sequence-based phylogenetic analysis was carried out on the actinobacterial isolates to determine their genetic relatedness to known actinobacteria. BOX-PCR analysis revealed high genetic diversity among the isolates. Several isolates were identified to belong to the genus Streptomyces and a few to Kitasatospora. The actinobacterial strains exhibited high diversity in their functionality and were identified as novel and promising candidates for future development into biocontrol and PGP agents.
authors.affiliation: Center for Nutraceutical and Pharmaceutical Materials, Myongji University, Cheoin-gu, Yongin, Gyeonggi-Do, South Korea; Division of Bioscience and Bioinformatics, College of Natural Science, Myongji University, Cheoin-gu, Yongin, Gyeonggi-Do, South Korea.
authors.forename: Sasikumar
authors.initials: S
authors.lastname: Arunachalam Palaniyandi
authors.forename: Seung Hwan
authors.initials: SH
authors.lastname: Yang
authors.forename: Karthiyaini
authors.initials: K
authors.lastname: Damodharan
authors.forename: Joo-Won
authors.initials: JW
authors.lastname: Suh
mesh_terms: D039903:Actinobacteria / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology*; D061046:Biological Control Agents; D004275:DNA, Ribosomal / Q000235:genetics; D027742:Dioscorea / Q000382:microbiology* / Q000502:physiology; D010802:Phylogeny; D058441:Rhizosphere
chemical_list: D061046:Biological Control Agents; D004275:DNA, Ribosomal
keywords: Actinobacteria; BOX-PCR; Biocontrol; Plant growth promotion; Rhizosphere; Yam