title: Matrix-based gene delivery for tissue repair.
abstract: Scaffolds for tissue repair must provide structural and biochemical cues to initiate the complex cascade of events that lead to proper tissue formation. Incorporating genes into these scaffolds is an attractive alternative to protein delivery since gene delivery can be tunable to any DNA sequence and genes utilize the cells' machinery to continuously produce therapeutic proteins, leading to longer lasting transgene expression and activation of autocrine and paracrine signaling that are not activated with bulk protein delivery. In this review, we discuss the importance of scaffold design and the impact of its design parameters (e.g. material, architecture, vector incorporation, biochemical cue presentation) on transgene expression and tissue repair.
authors.affiliation: Department of Bioengineering, University of California, Los Angeles, United States.
authors.forename: Cynthia
authors.initials: C
authors.lastname: Cam
authors.forename: Tatiana
authors.initials: T
authors.lastname: Segura
mesh_terms: D000042:Absorption; D000818:Animals; D004247:DNA / Q000235:genetics / Q000378:metabolism; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D061925:Immobilized Nucleic Acids / Q000235:genetics* / Q000378:metabolism*; D014162:Transfection / Q000379:methods; D019076:Transgenes / Q000235:genetics; D014945:Wound Healing / Q000235:genetics*
chemical_list: D061925:Immobilized Nucleic Acids; D004247:DNA