title: Modern principles of classification and development of nutrient media for culturing of human and animal cells.
abstract: Analysis of the main principles of classification and development of nutrient media used for culturing of human and animal cells in biology and medicine is presented. The key moments of induction and regulation of mitogenic cascades and their differences in cells of continuous lines, diploid cells, and primary cultures are discussed. Some variants of classification of nutrient media for various cell culture types are described. Peculiarities of composition and the prospects of using serum-free media are discussed. Practical results obtained by the authors in the development of diverse purpose nutrient media are presented.
authors.affiliation: Medical Genetic Research Center, Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: V Yu
authors.initials: VY
authors.lastname: Tabakov
authors.forename: Yu V
authors.initials: YV
authors.lastname: Schepkina
authors.forename: V V
authors.initials: VV
authors.lastname: Chestkov
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002460:Cell Line; D003470:Culture Media / Q000737:chemistry / Q000145:classification; D016895:Culture Media, Serum-Free; D006801:Humans
chemical_list: D003470:Culture Media; D016895:Culture Media, Serum-Free