title: Protection of cyanidin-3-glucoside against oxidative stress induced by acrylamide in human MDA-MB-231 cells.
abstract: Acrylamide (AA) occurs in many cooked starchy foods and has caused widespread concern as a possible carcinogen. In the present study, we investigate the intervention of AA toxicity in MDA-MB-231 cells pretreated with cyanidin-3-glucoside (Cy-3-glu). Compared to the cells treated with AA, Cy-3-glu significantly inhibited AA-induced cytotoxicity, reduced reactive oxygen species (ROS) generation, recovered glutathione (GSH) depletion and decreased the activities of glutathione peroxidase (GPx) and glutathione S-transferase (GST). Moreover, the expression of GPx1, GSTP1 and gamma-glutamyl cysteine synthase (γ-GCS) were enhanced, and cytochrome P450 2E1 (CYP2E1) expression was inhibited by the pretreatment of Cy-3-glu. Cy-3-glu presents the protective role against oxidative stress induced by AA in MDA-MB-231 cells.
authors.affiliation: College of Food Science and Nutritional Engineering, National Engineering Research Center for Fruit and Vegetable Processing, Key Laboratory of Fruits and Vegetables Processing, Ministry of Agriculture, Engineering Research Centre for Fruits and Vegetables Processing, Ministry of Education, China Agricultural University, No. 17, Qinghua East Road, Haidian District, Beijing 100083, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Song
authors.forename: Mengyao
authors.initials: M
authors.lastname: Zhao
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Zhu
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Hu
authors.forename: Fang
authors.initials: F
authors.lastname: Chen
mesh_terms: D020106:Acrylamide / Q000633:toxicity*; D000872:Anthocyanins / Q000494:pharmacology*; D045744:Cell Line, Tumor; D005960:Glucosides / Q000494:pharmacology*; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects*
chemical_list: D000872:Anthocyanins; D005960:Glucosides; C462279:cyanidin-3-O-beta-glucopyranoside; D020106:Acrylamide
keywords: 2′,7′-dichlorofluoresceindiacetate; AA; Acrylamide; CYP2E1; Cy-3-glu; Cyanidin-3-glucoside; Cytochrome P450 2E1; Cytotoxicity; DCFH-DA; GA; GPx; GSH; GST; Glutathione; Intervention; LDH; ROS; acrylamide; cyanidin-3-glucoside; cytochrome P450 2E1; gamma-glutamyl cysteine synthase; glutathione; glutathione S-transferase; glutathione peroxidase; glycidamide; lactate dehydrogenase; reactive oxygen species; γ-GCS