title: Self-assembled magnetic resonance imaging nanoprobes based on arachidyl chitosan for cancer diagnosis.
abstract: Arachidyl chitosan (chitosan oligosaccharide-arachidic acid; CSOAA)-based self-assembled nanoprobes for magnetic resonance imaging (MRI) of neoplastic lesions was developed and evaluated in vitro. Diethylenetriaminepentaacetic dianhydride (DTPA) was conjugated to chitosan oligosaccharide (CSO) and Gd(3+) was chelated to the resulting ligand. DTPA conjugation and Gd(3+) chelation were confirmed primarily by Fourier transform infrared spectroscopy (FT-IR) and zeta potential measurement. A spherical nanoprobe of around 150 nm mean diameter in the tested concentration range was formed in an aqueous environment by simple dissolution. The critical aggregation concentration (CAC) of the CSOAA-based nanoprobe was 3.86 μg/ml, indicating its stability after dilution in body fluid. The nanoprobe had negligible toxicity in head and neck cancer cell lines (Hep-2 and FaDu cells). The amount of Cy5.5-labeled nanoprobe taken-up by cells, as observed by confocal laser scanning microscopy (CLSM), increased according to incubation time (up to 12h). A phantom study showed a T1-positive contrast-enhancing effect of the developed CSOAA-based nanoprobe, compared to that of the commercial formulation (Gd-DTPA; Magnevist). These results indicate that the CSOAA-based nanoprobe can be used for efficient MR imaging of neoplastic cells.
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Ubonvan
authors.initials: U
authors.lastname: Termsarasab
authors.forename: Hyun-Jong
authors.initials: HJ
authors.lastname: Cho
authors.forename: Hyun Tae
authors.initials: HT
authors.lastname: Moon
authors.forename: Ju-Hwan
authors.initials: JH
authors.lastname: Park
authors.forename: In-Soo
authors.initials: IS
authors.lastname: Yoon
authors.forename: Dae-Duk
authors.initials: DD
authors.lastname: Kim
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry; D004537:Eicosanoic Acids / Q000737:chemistry; D006258:Head and Neck Neoplasms / Q000175:diagnosis*; D006801:Humans; D008279:Magnetic Resonance Imaging; D051379:Mice; D015394:Molecular Structure; D050997:Nanomedicine / Q000379:methods*; D053758:Nanoparticles / Q000737:chemistry; D009844:Oligosaccharides / Q000737:chemistry; D010316:Particle Size; D013499:Surface Properties; D023041:Xenograft Model Antitumor Assays
chemical_list: D004537:Eicosanoic Acids; D009844:Oligosaccharides; D048271:Chitosan; C094477:arachidic acid