title: Survival of Lactobacillus delbrueckii UFV H2b20 in fermented milk under simulated gastric and intestinal conditions.
abstract: The survival of Lactobacillus delbrueckii UFV H2b20 was assessed in fermented milk, both during the storage period and after exposure to simulated gastric and intestinal juices, as well the detection of the gene fbpA involved in adherence to human gastrointestinal tract. L. delbrueckii UFV H2b20 remained stable and viable for 28 days under refrigerated storage conditions. After one day of storage, that strain exhibited a one-log population reduction following exposure in tandem to simulated gastric and intestinal juices. After 14 days of storage, a two-log reduction was observed following 90 min of exposure to the simulated gastric conditions. However, the strain did not survive following exposure to the simulated intestinal juice. The observed tolerance to storage conditions and resistance to the simulated gastric and intestinal conditions confirm the potential use of L. delbrueckii UFV H2b20 as a probiotic, which is further reinforced by the detection of fbpA in this strain.
authors.affiliation: Department of Microbiology, Universidade Federal de Viçosa, 36571-000 Viçosa, MG, Brazil.
authors.forename: L L
authors.initials: LL
authors.lastname: da Conceição
authors.forename: E S
authors.initials: ES
authors.lastname: Leandro
authors.forename: F S
authors.initials: FS
authors.lastname: Freitas
authors.forename: M N V
authors.initials: MN
authors.lastname: de Oliveira
authors.forename: A B
authors.initials: AB
authors.lastname: Ferreira-Machado
authors.forename: A C
authors.initials: AC
authors.lastname: Borges
authors.forename: C A
authors.initials: CA
authors.lastname: de Moraes
mesh_terms: D000818:Animals; D015169:Colony Count, Microbial; D041981:Gastrointestinal Tract / Q000382:microbiology*; D006801:Humans; D048668:Lactobacillus delbrueckii / Q000502:physiology*; D050296:Microbial Viability; D008892:Milk / Q000382:microbiology*; D008962:Models, Theoretical; D019936:Probiotics