title: Polyphenolic pattern and in vitro cardioprotective properties of typical red wines from vineyards cultivated in Scafati (Salerno, Italy).
abstract: Wines are the subject of increasing numbers of investigations owing to the pharmaceutical usefulness of grape phytochemicals. The aim of the present work was to hypothesize the use of lyophilised red wines for the formulation of food supplements potentially useful against both physiological and induced cardiac oxidative stress. Cardiac derived H9C2 myocytes were incubated with increasing doses (0.01-1μg) of lyophilised Aglianico wine (lioAW). Experiments showed an appreciable direct radical scavenging activity at a maximum lioAW dose of 0.03μg that made the caspase-3 activity decrease by about 41%. Cardiac cells were exposed to 1μM doxorubicin and its combination with different doses of lioAW. Maximum lioAW aliquot of 0.03μg seemed to effectively contrast the induced oxidant injury decreasing the reactive oxygen species (ROSs) levels by about 38%and depressing the caspase-3 activity by about 63%. In both assays, pro-oxidant effects at higher lioAW concentrations were detected.
authors.affiliation: Department of Medicinal Chemistry, Università di Napoli Federico II, Via D. Montesano 49, 80131 Napoli, Italy. giancarlo.tenore@unina.it
authors.forename: Gian Carlo
authors.initials: GC
authors.lastname: Tenore
authors.forename: Michele
authors.initials: M
authors.lastname: Manfra
authors.forename: Paola
authors.initials: P
authors.lastname: Stiuso
authors.forename: Luigi
authors.initials: L
authors.lastname: Coppola
authors.forename: Mariateresa
authors.initials: M
authors.lastname: Russo
authors.forename: Alberto
authors.initials: A
authors.lastname: Ritieni
authors.forename: Pietro
authors.initials: P
authors.lastname: Campiglia
mesh_terms: D000818:Animals; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D007558:Italy; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D059808:Polyphenols / Q000032:analysis* / Q000494:pharmacology; D020011:Protective Agents / Q000032:analysis* / Q000494:pharmacology; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D027843:Vitis / Q000737:chemistry* / Q000254:growth & development; D014920:Wine / Q000032:analysis*
chemical_list: D059808:Polyphenols; D020011:Protective Agents; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase; D053148:Caspase 3