title: Chemical composition and in vitro antitrypanosomal activity of fractions of essential oil from Cymbopogon nardus L.
abstract: Essential oil from Cymbopogon nardus was evaluated for activity against Trypanosoma brucei brucei BS221 (IC50 = 0.31 ± 0.03 μg/mL) and cytotoxic effect on normal kidney (Vero) cells (IC50 = >100 μg/mL). The crude essential oil was subjected to various chromatography techniques afforded active sub fractions with antitrypanosomal activity; F4 (IC50 = 0.61 ± 0.06 μg/mL), F6 (IC50= 0.73 ± 0.33 μg/mL), F7 (IC50 = 1.15 ± 0 μg/mL) and F8 (IC50 = 1.11 ± 0.01 μg/mL). These active fractions did not exhibit any toxic effects against Vero cell lines and the chemical profiles investigation indicated presence of α-and γ-eudesmol, elemol, α-cadinol and eugenol by GC/MS analysis.
authors.affiliation: Natural Product Division, Forest Research Institute Malaysia, 52109 Kepong, Selangor, Malaysia. haffiz@frim.gov.my
authors.forename: J
authors.initials: J
authors.lastname: Muhd Haffiz
authors.forename: I
authors.initials: I
authors.lastname: Norhayati
authors.forename: K
authors.initials: K
authors.lastname: Getha
authors.forename: M A
authors.initials: MA
authors.lastname: Nor Azah
authors.forename: A
authors.initials: A
authors.lastname: Mohd Ilham
authors.forename: H
authors.initials: H
authors.lastname: Lili Sahira
authors.forename: M S
authors.initials: MS
authors.lastname: Roshan Jahn
authors.forename: A
authors.initials: A
authors.lastname: Muhd Syamil
mesh_terms: D000818:Animals; D000981:Antiprotozoal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D031743:Cymbopogon / Q000737:chemistry*; D004847:Epithelial Cells / Q000187:drug effects / Q000502:physiology; D008401:Gas Chromatography-Mass Spectrometry; D020128:Inhibitory Concentration 50; D009822:Oils, Volatile / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D021261:Parasitic Sensitivity Tests; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D014346:Trypanosoma brucei brucei / Q000187:drug effects*; D014709:Vero Cells
chemical_list: D000981:Antiprotozoal Agents; D009822:Oils, Volatile; D010936:Plant Extracts