title: N-glycosylamine-mediated isotope labeling for mass spectrometry-based quantitative analysis of N-linked glycans.
abstract: N-linked glycosylation is a major protein modification involved in many essential cellular functions. Methods capable of quantitative glycan analysis are highly valuable and have been actively pursued. Here we describe a novel N-glycosylamine-based strategy for isotopic labeling of N-linked glycans for quantitative analysis by use of mass spectrometry (MS). This strategy relies on the primary amine group on the reducing end of freshly released N-linked glycans for labeling, and eliminates the need for the harsh labeling reaction conditions and/or tedious cleanup procedures required by existing methods. By using NHS-ester amine chemistry we used this strategy to label N-linked glycans from a monoclonal antibody with commercially available tandem mass tags (TMT). Only duplex experiments can be performed with currently available TMT reagents, because quantification is based on the intensity of intact labeled glycans. Under mild reaction conditions, greater than 95% derivatization was achieved in 30 min and the labeled glycans, when kept at -20 °C, were stable for more than 10 days. By performing glycan release, TMT labeling, and LC-MS analysis continuously in a single volatile aqueous buffer without cleanup steps, we were able to complete the entire analysis in less than 2 h. Quantification was highly accurate and the dynamic range was large. Compared with previously established methods, N-glycosylamine-mediated labeling has the advantages of experimental simplicity, efficient labeling, and preserving glycan integrity.
authors.affiliation: GlycoFi, Biologics Discovery, Merck & Co. Inc., 16 Cavendish Court, Lebanon, NH 03766, USA. bing.gong@merck.com
authors.forename: Bing
authors.initials: B
authors.lastname: Gong
authors.forename: Erik
authors.initials: E
authors.lastname: Hoyt
authors.forename: Heather
authors.initials: H
authors.lastname: Lynaugh
authors.forename: Irina
authors.initials: I
authors.lastname: Burnina
authors.forename: Renee
authors.initials: R
authors.lastname: Moore
authors.forename: Alissa
authors.initials: A
authors.lastname: Thompson
authors.forename: Huijuan
authors.initials: H
authors.lastname: Li
mesh_terms: D000588:Amines / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry*; D006031:Glycosylation; D007553:Isotope Labeling; D013058:Mass Spectrometry / Q000379:methods*; D009587:Nitrogen Isotopes / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: D000588:Amines; D006023:Glycoproteins; D009587:Nitrogen Isotopes; D011134:Polysaccharides