title: Pre-clinical anti-inflammatory aspects of a cuisine and medicinal millennial herb: Malva sylvestris L.
abstract: Malva sylvestris has been used since ancient times for its emollient, laxative and anti-inflammatory properties, being extensively used as salads, soups and teas. The preset study evaluated the topical anti-inflammatory action of M. sylvestris hydroalcoholic extract (HE) and its compounds in mice ear inflammation caused by 12-O-tetradecanoylphorbol-acetate in mice. The LC-MS analysis of the HE confirmed the presence of scopoletin, quercetin and malvidin 3-glucoside compounds in the HE of M. sylvestris. Topical application of the HE reduced ear oedema, polymorphonuclear cells influx (myeloperoxydase activity and histological analysis) and interleukin-1β levels in the tissue. The topical application of the compound present in the HE, malvidin 3-glucoside was also able to inhibit ear oedema and leukocytes migration. The other tested compounds, scopoletin, quercetin and malvidin 3,5-glucoside were able to prevent the formation of oedema and cell infiltration, but with less effectiveness when compared to HE and malvidin 3-glucoside. Therefore, these results consistently support the notion that M. sylvestris leaves possesses topical anti-inflammatory activity, the compound malvidin 3-glucoside seems to be major responsible for this effect, with the participation of other anti-inflammatory compounds in the extract. Thus, as recommended by population, M. sylvestris can be used as a future treatment to skin disorders.
authors.affiliation: Department of Pharmacology, Universidade Federal do Paraná, Centro Politécnico, 81531-900 Curitiba, PR, Brazil.
authors.forename: Arthur S
authors.initials: AS
authors.lastname: Prudente
authors.forename: Alliete M V
authors.initials: AM
authors.lastname: Loddi
authors.forename: Márcia R
authors.initials: MR
authors.lastname: Duarte
authors.forename: Adair R S
authors.initials: AR
authors.lastname: Santos
authors.forename: Marcia T
authors.initials: MT
authors.lastname: Pochapski
authors.forename: Moacir G
authors.initials: MG
authors.lastname: Pizzolatti
authors.forename: Sirlei S
authors.initials: SS
authors.lastname: Hayashi
authors.forename: Francinete R
authors.initials: FR
authors.lastname: Campos
authors.forename: Roberto
authors.initials: R
authors.lastname: Pontarolo
authors.forename: Fabio A
authors.initials: FA
authors.lastname: Santos
authors.forename: Daniela A
authors.initials: DA
authors.lastname: Cabrini
authors.forename: Michel F
authors.initials: MF
authors.lastname: Otuki
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D002853:Chromatography, Liquid; D005260:Female; D053583:Interleukin-1beta / Q000378:metabolism; D028501:Malva / Q000737:chemistry*; D051379:Mice; D009195:Peroxidase / Q000378:metabolism; D010946:Plants, Medicinal; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000893:Anti-Inflammatory Agents; D053583:Interleukin-1beta; D009195:Peroxidase
keywords: Ear oedema; Interleukin-1β; Malva sylvestris; Mouse; Skin; Topic anti-inflammatory