title: Actinopolyspora mzabensis sp. nov., a halophilic actinomycete isolated from an Algerian Saharan soil.
abstract: A halophilic actinomycete strain, designated H55(T), was isolated from Saharan soil sampled in the Mzab region (Ghardaïa, southern Algeria) and was characterized in a taxonomic study using a polyphasic approach. The cell wall was determined to contain meso-diaminopimelic acid and the characteristic whole-cell sugars were arabinose and galactose. The predominant menaquinones were found to be MK-10(H4) and MK-9(H4). The predominant cellular fatty acids were determined to be anteiso-C17 : 0, iso-C16 : 0 and iso-C15 : 0. The diagnostic phospholipid detected was phosphatidylcholine. The morphological and chemotaxonomic characteristics of the strain were consistent with those of members of the genus Actinopolyspora, and 16S rRNA gene sequence analysis confirmed that strain H55(T) was a member of this genus. DNA-DNA hybridization values between strain H55(T) and the type strains of the nearest species of the genus Actinopolyspora, Actinopolyspora erythraea and A. alba, were clearly below the 70 % threshold. The genotypic and phenotypic data showed that the organism represents a novel species of the genus Actinopolyspora, for which the name Actinopolyspora mzabensis sp. nov. is proposed, with the type strain H55(T) ( = DSM 45460(T) = CCUG 62965(T)).
authors.affiliation: Faculté des Sciences Agronomiques, Vétérinaires et Biologiques, Département de Biologie, Université Saâd Dahleb de Blida, Algeria.|Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algeria.
authors.forename: Atika
authors.initials: A
authors.lastname: Meklat
authors.affiliation: Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algeria.
authors.forename: Noureddine
authors.initials: N
authors.lastname: Bouras
authors.affiliation: Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algeria.
authors.forename: Abdelghani
authors.initials: A
authors.lastname: Zitouni
authors.affiliation: Université de Toulouse; INPT-ENSAT, Laboratoire de Génie Chimique; UMR 5503 (CNRS/INPT/UPS), 1 Avenue de l'Agrobiopôle, BP 32607 Auzeville Tolosane, 31326 Castanet-Tolosan, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Mathieu
authors.affiliation: Université Moulay Ismail, Marjane 2, BP 298, Meknes, Morocco.|Université de Toulouse; INPT-ENSAT, Laboratoire de Génie Chimique; UMR 5503 (CNRS/INPT/UPS), 1 Avenue de l'Agrobiopôle, BP 32607 Auzeville Tolosane, 31326 Castanet-Tolosan, France.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Lebrihi
authors.affiliation: Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Schumann
authors.affiliation: Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
authors.forename: Cathrin
authors.initials: C
authors.lastname: Spröer
authors.affiliation: Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Klenk
authors.affiliation: Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algeria.
authors.forename: Nasserdine
authors.initials: N
authors.lastname: Sabaou
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001089:Arabinose / Q000032:analysis; D015373:Bacterial Typing Techniques; D002473:Cell Wall / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D003889:Desert Climate; D003960:Diaminopimelic Acid / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005690:Galactose / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010713:Phosphatidylcholines / Q000032:analysis; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010713:Phosphatidylcholines; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid; D001089:Arabinose; D005690:Galactose