title: 3DEM Loupe: Analysis of macromolecular dynamics using structures from electron microscopy.
abstract: Electron microscopy (EM) provides access to structural information of macromolecular complexes in the 3-20 Å resolution range. Normal mode analysis has been extensively used with atomic resolution structures and successfully applied to EM structures. The major application of normal modes is the identification of possible conformational changes in proteins. The analysis can throw light on the mechanism following ligand binding, protein-protein interactions, channel opening and other functional macromolecular movements. In this article, we present a new web server, 3DEM Loupe, which allows normal mode analysis of any uploaded EM volume using a user-friendly interface and an intuitive workflow. Results can be fully explored in 3D through animations and movies generated by the server. The application is freely available at http://3demloupe.cnb.csic.es.
authors.affiliation: National Center for Biotechnology-CSIC, Madrid 28049, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Nogales-Cadenas
authors.forename: S
authors.initials: S
authors.lastname: Jonic
authors.forename: F
authors.initials: F
authors.lastname: Tama
authors.forename: A A
authors.initials: AA
authors.lastname: Arteni
authors.forename: D
authors.initials: D
authors.lastname: Tabas-Madrid
authors.forename: M
authors.initials: M
authors.lastname: Vázquez
authors.forename: A
authors.initials: A
authors.lastname: Pascual-Montano
authors.forename: C O S
authors.initials: CO
authors.lastname: Sorzano
mesh_terms: D020407:Internet; D046911:Macromolecular Substances / Q000737:chemistry / Q000648:ultrastructure*; D008854:Microscopy, Electron; D011487:Protein Conformation; D012984:Software
chemical_list: D046911:Macromolecular Substances