title: Control of post-translational modifications in antithrombin during murine post-natal development by miR-200a.
abstract: BACKGROUND
  Developmental haemostatic studies may help identifying new elements involved in the control of key haemostatic proteins like antithrombin, the most relevant endogenous anticoagulant.
  
  
  RESULTS
  In this study, we showed a significant reduction of sialic acid content in neonatal antithrombin compared with adult antithrombin in mice. mRNA levels of St3gal3 and St3gal4, two sialyltransferases potentially involved in antithrombin sialylation, were 85% lower in neonates in comparison with adults. In silico analysis of miRNAs overexpressed in neonates revealed that mir-200a might target these sialyltransferases. Moreover, in vitro studies in murine primary hepatocytes sustain this potential control.
  
  
  CONCLUSIONS
  These data suggest that in addition to the direct protein regulation, microRNAs may also modulate qualitative traits of selected proteins by an indirect control of post-translational processes.
authors.affiliation: Centro Regional de Hemodonación, University of Murcia, IMIB, Spain, C/Ronda de Garay S/N, 30003, Murcia, Spain.
authors.forename: Raúl
authors.initials: R
authors.lastname: Teruel
authors.forename: Irene
authors.initials: I
authors.lastname: Martínez-Martínez
authors.forename: José A
authors.initials: JA
authors.lastname: Guerrero
authors.forename: Rocío
authors.initials: R
authors.lastname: González-Conejero
authors.forename: María E
authors.initials: ME
authors.lastname: de la Morena-Barrio
authors.forename: Salam
authors.initials: S
authors.lastname: Salloum-Asfar
authors.forename: Ana B
authors.initials: AB
authors.lastname: Arroyo
authors.forename: Sonia
authors.initials: S
authors.lastname: Águila
authors.forename: Nuria
authors.initials: N
authors.lastname: García-Barberá
authors.forename: Antonia
authors.initials: A
authors.lastname: Miñano
authors.forename: Vicente
authors.initials: V
authors.lastname: Vicente
authors.forename: Javier
authors.initials: J
authors.lastname: Corral
authors.forename: Constantino
authors.initials: C
authors.lastname: Martínez
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D000991:Antithrombins / Q000378:metabolism*; D020869:Gene Expression Profiling; D022781:Hepatocytes / Q000378:metabolism; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D012333:RNA, Messenger / Q000378:metabolism; D012799:Sialyltransferases / Q000235:genetics / Q000378:metabolism; D000094663:beta-Galactoside alpha-2,3-Sialyltransferase
chemical_list: D000991:Antithrombins; D035683:MicroRNAs; C543719:Mirn200 microRNA, mouse; D012333:RNA, Messenger; D012799:Sialyltransferases; D019158:N-Acetylneuraminic Acid; D000094663:beta-Galactoside alpha-2,3-Sialyltransferase