title: Calcium isolation from large-volume human urine samples for 41Ca analysis by accelerator mass spectrometry.
abstract: Calcium oxalate precipitation is the first step in preparation of biological samples for (41)Ca analysis by accelerator mass spectrometry. A simplified protocol for large-volume human urine samples was characterized, with statistically significant increases in ion current and decreases in interference. This large-volume assay minimizes cost and effort and maximizes time after (41)Ca administration during which human samples, collected over a lifetime, provide (41)Ca:Ca ratios that are significantly above background.
authors.affiliation: Argonne National Laboratory, 9700 South Cass Avenue, Lemont, IL 60439, USA.
authors.forename: James J
authors.initials: JJ
authors.lastname: Miller
authors.forename: Susanta K
authors.initials: SK
authors.lastname: Hui
authors.forename: George S
authors.initials: GS
authors.lastname: Jackson
authors.forename: Sara P
authors.initials: SP
authors.lastname: Clark
authors.forename: Jane
authors.initials: J
authors.lastname: Einstein
authors.forename: Connie M
authors.initials: CM
authors.lastname: Weaver
authors.forename: Maryka H
authors.initials: MH
authors.lastname: Bhattacharyya
mesh_terms: D002118:Calcium / Q000302:isolation & purification* / Q000652:urine*; D002132:Calcium Radioisotopes / Q000737:chemistry / Q000652:urine*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D013058:Mass Spectrometry / Q000295:instrumentation*; D010315:Particle Accelerators / Q000295:instrumentation*; D013048:Specimen Handling / Q000295:instrumentation*; D016482:Urinalysis / Q000295:instrumentation*
chemical_list: D002132:Calcium Radioisotopes; D002118:Calcium