title: Therapeutic approaches of melatonin in microwave radiations-induced oxidative stress-mediated toxicity on male fertility pattern of Wistar rats.
abstract: Microwave (MW) radiation produced by wireless telecommunications and a number of electrical devices used in household or in healthcare institutions may adversely affects the reproductive pattern. Present study aimed to investigate the protective effects of melatonin (is well known antioxidant that protects DNA, lipids and proteins from free radical damage) against oxidative stress-mediated testicular impairment due to long-term exposure of MWs. For this, 70-day-old male Wistar rats were divided into four groups (n = 6/group): Sham exposed, Melatonin (Mel) treated (2 mg/kg), 2.45 GHz MWs exposed and MWs + Mel treated. Exposure took place in Plexiglas cages for 2 h a day for 45 days where, power density (0.21 mW/cm(2)) and specific absorption rate (SAR 0.14 W/Kg) were estimated. After the completion of exposure period, rats were sacrificed and various stress related parameters, that is LDH-X (lactate dehydrogenase isoenzyme) activity, xanthine oxidase (XO), ROS (reactive oxygen species), protein carbonyl content, DNA damage and MDA (malondialdehyde) were performed. Result shows that melatonin prevent oxidative damage biochemically by significant increase (p < 0.001) in the levels of testicular LDH-X, decreased (p < 0.001) levels of MDA and ROS in testis (p < 0.01). Meanwhile, it reversed the effects of MWs on XO, protein carbonyl content, sperm count, testosterone level and DNA fragmentation in testicular cells. These results concluded that the melatonin has strong antioxidative potential against MW induced oxidative stress mediated DNA damage in testicular cells.
authors.affiliation: School of Environmental Sciences, Jawaharlal Nehru University , New Delhi , India .
authors.forename: Ramovatar
authors.initials: R
authors.lastname: Meena
authors.forename: Kajal
authors.initials: K
authors.lastname: Kumari
authors.forename: Jitendra
authors.initials: J
authors.lastname: Kumar
authors.forename: Paulraj
authors.initials: P
authors.lastname: Rajamani
authors.forename: H N
authors.initials: HN
authors.lastname: Verma
authors.forename: Kavindra Kumar
authors.initials: KK
authors.lastname: Kesari
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D001835:Body Weight / Q000187:drug effects / Q000528:radiation effects; D004249:DNA Damage; D005298:Fertility / Q000187:drug effects* / Q000528:radiation effects*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008550:Melatonin / Q000494:pharmacology*; D008872:Microwaves / Q000009:adverse effects*; D018384:Oxidative Stress / Q000187:drug effects* / Q000528:radiation effects*; D050050:Protein Carbonylation / Q000187:drug effects; D011837:Radiation-Protective Agents / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013737:Testis / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D013739:Testosterone / Q000378:metabolism; D014969:Xanthine Oxidase / Q000378:metabolism
chemical_list: D011837:Radiation-Protective Agents; D013739:Testosterone; D008315:Malondialdehyde; D007770:L-Lactate Dehydrogenase; D014969:Xanthine Oxidase; D008550:Melatonin