title: Enantioseparations of amino acids by capillary array electrophoresis with 532 nm laser induced fluorescence detection.
abstract: Capillary array electrophoresis (CAE) is a promising technique for multiple enantiomeric separations. Carboxytetramethylrhodamine succinimidyl ester (TAMRA SE), a rhodamine-core fluorescent probe, has rarely been applied as an original precolumn derivatization reagent for chiral amino acid (AA) analysis so far. For these purposes, high-throughput enantiomeric separations of 12 TAMRA SE-AAs by a home-made 532 nm CAE-LIF scanner are presented. The effect of cyclodextrins (CDs) and a variety of organic modifiers was quickly investigated. Baseline separations were achieved in 100 mM Tris-borate buffer (pH 10.0) containing 2 mM β-CD and 10 mM hexamethylenediamine (HDA). Multiple determination of the enantiomeric excess (ee) in non-racemic mixtures of alanine is successfully presented.
authors.affiliation: Institute of Environmental Engineering, Department of Physics, Dalian Maritime University, 1 Linghai Road, Dalian 116026, China. kyliu@cas.dicp.ac.cn
authors.forename: Kaiying
authors.initials: K
authors.lastname: Liu
authors.forename: Li
authors.initials: L
authors.lastname: Wang
mesh_terms: D000596:Amino Acids / Q000737:chemistry* / Q000302:isolation & purification*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D005453:Fluorescence; D013237:Stereoisomerism
chemical_list: D000596:Amino Acids