title: Lipidomic analysis of plant membrane lipids by direct infusion tandem mass spectrometry.
abstract: Plant phospholipids and glycolipids can be analyzed by direct infusion electrospray ionization triple-quadrupole mass spectrometry. A biological extract is introduced in solvent by continuous infusion into the mass spectrometer's electrospray ionization source, where ions are produced from the lipids. For analysis of membrane lipids, a series of precursor and neutral loss scans, each specific for lipids containing a common head group, are obtained sequentially. The mass spectral data are processed and combined, using the Web application LipidomeDB Data Calculation Environment, to create a lipid profile.
authors.affiliation: Division of Biology, Kansas Lipidomics Research Center, Kansas State University, Manhattan, KS, USA.
authors.forename: Sunitha
authors.initials: S
authors.lastname: Shiva
authors.forename: Hieu Sy
authors.initials: HS
authors.lastname: Vu
authors.forename: Mary R
authors.initials: MR
authors.lastname: Roth
authors.forename: Zhenguo
authors.initials: Z
authors.lastname: Zhou
authors.forename: Shantan Reddy
authors.initials: SR
authors.lastname: Marepally
authors.forename: Daya Sagar
authors.initials: DS
authors.lastname: Nune
authors.forename: Gerald H
authors.initials: GH
authors.lastname: Lushington
authors.forename: Mahesh
authors.initials: M
authors.lastname: Visvanathan
authors.forename: Ruth
authors.initials: R
authors.lastname: Welti
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D050356:Lipid Metabolism; D008563:Membrane Lipids / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification; D012015:Reference Standards; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D008563:Membrane Lipids