title: A synthetic approach to GFP chromophore analogs from 3-azidocinnamates. Role of methyl rotors in chromophore photophysics.
abstract: We have suggested a novel combinatorial approach for synthesis of otherwise inaccessible GFP chromophore analogs, and studied the influence of aliphatic substituents on their pH-dependent spectral properties. We found that the demethylation at C or N positions of the imidazolone ring leads to a decrease in the excited state lifetime.
authors.affiliation: Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, 117997 Moscow, Russia.
authors.forename: Mikhail S
authors.initials: MS
authors.lastname: Baranov
authors.forename: Kyril M
authors.initials: KM
authors.lastname: Solntsev
authors.forename: Konstantin A
authors.initials: KA
authors.lastname: Lukyanov
authors.forename: Ilia V
authors.initials: IV
authors.lastname: Yampolsky
mesh_terms: D002384:Catalysis; D002934:Cinnamates / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007093:Imidazoles / Q000737:chemistry; D007536:Isomerism; D007700:Kinetics; D013696:Temperature
chemical_list: D002934:Cinnamates; D007093:Imidazoles; C117197:imidazolone; D049452:Green Fluorescent Proteins