title: Apoptosis resistance in endometriosis.
abstract: BACKGROUND
  In a cytological analysis of endometriotic lesions neither granulocytes nor cytotoxic T-cells appear in an appreciable number. Based on this observation we aimed to know, whether programmed cell death plays an essential role in the destruction of dystopic endometrium. Disturbances of the physiological mechanisms of apoptosis, a persistence of endometrial tissue could explain the disease. Another aspect of this consideration is the proliferation competence of the dystopic mucous membrane.
  
  
  METHODS
  Endometriotic lesions of 15 patients were examined through a combined measurement of apoptosis activity with the TUNEL technique (terminal deoxyribosyltransferase mediated dUTP Nick End Labeling) and the proliferation activity (with the help of the Ki-67-Antigens using the monoclonal antibody Ki-S5).
  
  
  RESULTS
  Twelve out of 15 women studied showed a positive apoptotic activity of 3-47% with a proliferation activity of 2-25% of epithelial cells. Therefore we concluded that the persistence of dystopic endometrium requires proliferative epithelial cells from middle to lower endometrial layers.
  
  
  CONCLUSIONS
  A dystopia misalignment of the epithelia of the upper layers of the functionalism can be rapidly eliminated by apoptotic procedures.
authors.affiliation: Centre for Reproductive Medicine, Women's Hospital, Christian-Albrechts-University, Kiel, Germany.
authors.forename: Ali
authors.initials: A
authors.lastname: Salmassi
authors.forename: Bengi
authors.initials: B
authors.lastname: Acar-Perk
authors.forename: Andreas G
authors.initials: AG
authors.lastname: Schmutzler
authors.forename: Kerstin
authors.initials: K
authors.lastname: Koch
authors.forename: Frank
authors.initials: F
authors.lastname: Püngel
authors.forename: Walter
authors.initials: W
authors.lastname: Jonat
authors.forename: Liselotte
authors.initials: L
authors.lastname: Mettler
keywords: Apoptosis; Endometriosis; TUNEL Assay