title: Two-photon optical interrogation of individual dendritic spines with caged dopamine.
abstract: We introduce a novel caged dopamine compound (RuBi-Dopa) based on ruthenium photochemistry. RuBi-Dopa has a high uncaging efficiency and can be released with visible (blue-green) and IR light in a two-photon regime. We combine two-photon photorelease of RuBi-Dopa with two-photon calcium imaging for an optical imaging and manipulation of dendritic spines in living brain slices, demonstrating that spines can express functional dopamine receptors. This novel compound allows mapping of functional dopamine receptors in living brain tissue with exquisite spatial resolution.
authors.forename: Roberto
authors.initials: R
authors.lastname: Araya
authors.forename: Victoria
authors.initials: V
authors.lastname: Andino-Pavlovsky
authors.forename: Rafael
authors.initials: R
authors.lastname: Yuste
authors.forename: Roberto
authors.initials: R
authors.lastname: Etchenique
mesh_terms: D000818:Animals; D002127:Calcium Isotopes; D049229:Dendritic Spines / Q000378:metabolism / Q000502:physiology*; D004298:Dopamine; D051379:Mice; D010782:Photolysis; D017785:Photons; D017397:Prefrontal Cortex / Q000378:metabolism / Q000502:physiology*; D011954:Receptors, Dopamine / Q000378:metabolism / Q000502:physiology*; D012428:Ruthenium
chemical_list: D002127:Calcium Isotopes; D011954:Receptors, Dopamine; D012428:Ruthenium; D004298:Dopamine