title: Gene polymorphisms in African buffalo associated with susceptibility to bovine tuberculosis infection.
abstract: Bovine tuberculosis (BTB) is a chronic, highly infectious disease that affects humans, cattle and numerous species of wildlife. In developing countries such as South Africa, the existence of extensive wildlife-human-livestock interfaces poses a significant risk of Mycobacterium bovis transmission between these groups, and has far-reaching ecological, economic and public health impacts. The African buffalo (Syncerus caffer), acts as a maintenance host for Mycobacterium bovis, and maintains and transmits the disease within the buffalo and to other species. In this study we aimed to investigate genetic susceptibility of buffalo for Mycobacterium bovis infection. Samples from 868 African buffalo of the Cape buffalo subspecies were used in this study. SNPs (n = 69), with predicted functional consequences in genes related to the immune system, were genotyped in this buffalo population by competitive allele-specific SNP genotyping. Case-control association testing and statistical analyses identified three SNPs associated with BTB status in buffalo. These SNPs, SNP41, SNP137 and SNP144, are located in the SLC7A13, DMBT1 and IL1α genes, respectively. SNP137 remained significantly associated after permutation testing. The three genetic polymorphisms identified are located in promising candidate genes for further exploration into genetic susceptibility to BTB in buffalo and other bovids, such as the domestic cow. These polymorphisms/genes may also hold potential for marker-assisted breeding programmes, with the aim of breeding more BTB-resistant animals and herds within both the national parks and the private sector.
authors.affiliation: Division of Molecular Biology and Human Genetics, DST/NRF Centre of Excellence for Biomedical Tuberculosis Research, Faculty of Health Sciences, Stellenbosch University, Cape Town, South Africa. nikkileroex@sun.ac.za
authors.forename: Nikki
authors.initials: N
authors.lastname: le Roex
authors.forename: Ad P
authors.initials: AP
authors.lastname: Koets
authors.forename: Paul D
authors.initials: PD
authors.lastname: van Helden
authors.forename: Eileen G
authors.initials: EG
authors.lastname: Hoal
mesh_terms: D000349:Africa; D000818:Animals; D002020:Buffaloes / Q000235:genetics*; D002417:Cattle; D020022:Genetic Predisposition to Disease; D020641:Polymorphism, Single Nucleotide; D014380:Tuberculosis, Bovine / Q000235:genetics*