title: Expression of SLP-2 was associated with invasion of esophageal squamous cell carcinoma.
abstract: BACKGROUND
  Stomatin-like protein 2 (SLP-2), a member of the Stomatin superfamily, has been identified as an oncogenic-related protein and found to be up-regulated in multi-cancers. Nonetheless, the expression pattern and regulation of SLP-2 in human esophageal squamous cell carcinoma (ESCC) remain unexplored.
  
  
  METHODS
  Immunohistochemistry and immunofluorescence staining analysis were performed to show SLP-2 expression and location. RNAi method was used to inhibit specific protein expression. Transwell assay was done to investigate cells invasive capability. RT-PCR and Western blot analysis were used to detect mRNA and protein expression levels.
  
  
  RESULTS
  Immunohistochemical analysis showed that up-regulation of SLP-2 was found in invasive front compared with cancer central tissue in ESCC. Inhibition of SLP-2 by SLP-2 siRNA can decrease ESCC cells invasive capability through MMP-2 dependent manner. Up-regulation of SLP-2 was effectively abrogated by the ERK1/2 inhibitors either PD98059 or U0126, but no effect was showed by the treatment of AKT inhibitors either LY294002 or MK-2206. So the regulation of SLP-2 was involved in activation of the MAPK/ERK pathway.
  
  
  CONCLUSIONS
  We found that PMA/EGF could induce the up-regulated expression of SLP-2 probably through activating ERK signalling. The current study suggests that SLP-2 may represent an important molecular hallmark that is clinically relevant to the invasion of ESCC.
authors.affiliation: Key Laboratory of Cancer Prevention and Therapy, Department of Pathology, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China.
authors.forename: Wenfeng
authors.initials: W
authors.lastname: Cao
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.forename: Fang
authors.initials: F
authors.lastname: Ding
authors.forename: Weiran
authors.initials: W
authors.lastname: Zhang
authors.forename: Baocun
authors.initials: B
authors.lastname: Sun
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Liu
mesh_terms: D001798:Blood Proteins / Q000378:metabolism*; D015153:Blotting, Western; D002070:Butadienes; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000503:physiopathology; D004938:Esophageal Neoplasms / Q000378:metabolism* / Q000503:physiopathology; D005419:Flavonoids; D005455:Fluorescent Antibody Technique; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D020935:MAP Kinase Signaling System / Q000502:physiology; D008565:Membrane Proteins / Q000378:metabolism*; D009361:Neoplasm Invasiveness / Q000503:physiopathology*; D009570:Nitriles; D034622:RNA Interference
chemical_list: D001798:Blood Proteins; D002070:Butadienes; D005419:Flavonoids; D008565:Membrane Proteins; D009570:Nitriles; C406287:STOML2 protein, human; C113580:U 0126; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one