title: Production of the cannibalism toxin SDP is a multistep process that requires SdpA and SdpB.
abstract: During the early stages of sporulation, a subpopulation of Bacillus subtilis cells secrete toxins that kill their genetically identical siblings in a process termed cannibalism. One of these toxins is encoded by the sdpC gene of the sdpABC operon. The active form of the SDP toxin is a 42-amino-acid peptide with a disulfide bond which is processed from an internal fragment of pro-SdpC. The factors required for the processing of pro-SdpC into mature SDP are not known. We provide evidence that pro-SdpC is secreted via the general secretory pathway and that signal peptide cleavage is a required step in the production of SDP. We also demonstrate that SdpAB are essential to produce mature SDP, which has toxin activity. Our data indicate that SdpAB are not required for secretion, translation, or stability of SdpC. Thus, SdpAB may participate in a posttranslation step in the production of SDP. The mature form of the SDP toxin contains a disulfide bond. Our data indicate that while the disulfide bond does increase activity of SDP, it is not essential for SDP activity. We demonstrate that the disulfide bond is formed independently of SdpAB. Taken together, our data suggest that SDP production is a multistep process and that SdpAB are required for SDP production likely by controlling, directly or indirectly, cleavage of SDP from the pro-SdpC precursor.
authors.affiliation: Department of Microbiology, University of Iowa, Iowa City, IA, USA.
authors.forename: Tiara G
authors.initials: TG
authors.lastname: Pérez Morales
authors.forename: Theresa D
authors.initials: TD
authors.lastname: Ho
authors.forename: Wei-Ting
authors.initials: WT
authors.lastname: Liu
authors.forename: Pieter C
authors.initials: PC
authors.lastname: Dorrestein
authors.forename: Craig D
authors.initials: CD
authors.lastname: Ellermeier
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D001427:Bacterial Toxins / Q000235:genetics / Q000378:metabolism*; D011499:Protein Processing, Post-Translational
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins