title: Recombinant disintegrin domain of ADAM15 inhibits the proliferation and migration of Bel-7402 cells.
abstract: ADAM15 (A Disintegrin And Metalloproteinase 15), a transmembrane protein containing seven domains, interacts with some integrins via its disintegrin domain and overexpresses in many solid tumors. In this study, the effect of the recombinant human disintegrin domain (rhddADAM15) on the proliferation and migration of Bel-7402 cells was evaluated in vitro and in vivo in zebrafish xenografts. rhddADAM15 (4 μM) severely inhibited the proliferation and migration of Bel-7402 cells, inducing a partial G2/S arrest and morphological nucleus changes of apoptosis. Moreover, the activity of caspases 8, 9 and 3 in Bel-7402 cells was increased. In addition, the zebrafish was used as a model for apoptosis-induction and tumor-xenograft. rhddADAM15 (1 pM) inhibited the growth and metastasis of Bel-7402 cell xenografts in zebrafish and a lower concentration (0.1 pM) induced severe apoptosis in the somatic cells of zebrafish. In conclusion, our data identified rhddADAM15 as a potent inhibitor of tumor growth and metastasis, making it a promising tool for use in anticancer treatment.
authors.affiliation: Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Pharmaceutical Sciences, Jiangnan University, 1800 Lihu Rd., Wuxi, Jiangsu 214122, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Hou
authors.forename: M
authors.initials: M
authors.lastname: Chu
authors.forename: F F
authors.initials: FF
authors.lastname: Du
authors.forename: J Y
authors.initials: JY
authors.lastname: Lei
authors.forename: Y
authors.initials: Y
authors.lastname: Chen
authors.forename: R Y
authors.initials: RY
authors.lastname: Zhu
authors.forename: X H
authors.initials: XH
authors.lastname: Gong
authors.forename: X
authors.initials: X
authors.lastname: Ma
authors.forename: J
authors.initials: J
authors.lastname: Jin
mesh_terms: D051722:ADAM Proteins / Q000235:genetics / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D019483:Disintegrins / Q000235:genetics / Q000494:pharmacology*; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology* / Q000503:physiopathology*; D008565:Membrane Proteins / Q000235:genetics / Q000494:pharmacology*; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000494:pharmacology
chemical_list: D019483:Disintegrins; D008565:Membrane Proteins; D011994:Recombinant Proteins; D051722:ADAM Proteins; C098660:ADAM15 protein, human