title: Tissue expression, serum and salivary levels of IL-10 in patients with head and neck squamous cell carcinoma.
abstract: BACKGROUND
  Head and neck SCC is a common cancer related to various factors. IL-10, a pleiotropic cytokine produced by macrophages, T-helper-2 cells, and B lymphocytes, is thought to play a potential pathogenetic or therapeutic role in a number of human conditions, such as inflammation, autoimmunity and cancer. The present study was designed to evaluate the relation between tissue expression, serum and salivary levels of IL-10 in head and neck squamous cell carcinomas (HNSCCs) and their correlation with clinicopathologic features.
  
  
  METHODS
  Samples were collected from 30 patients with HNSCCs and 24 healthy volunteers. IHC analysis was used to examine the tissue expression and ELISA was employed to measure serum and salivary levels.
  
  
  RESULTS
  Our study showed tissue expression of IL-10 to be significantily higher in patients (P: 0.001), but there was no relation between tissue expression , serum and salivary levels of the marker (P>0.05). Also except for a positive correlation between tissue expression of IL-10 and stage (P: 0.044), there was no relation between this marker and clinicopathologic features. There was no correlation between serum and salivary levels in either patients or controls.
  
  
  CONCLUSIONS
  It seems there is no correlation between level of IL-10 in serum and saliva and this marker in saliva and serum does not reflect tissue expression.
authors.affiliation: Department of Oral and Maxillofacial Pathology, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran. azadehandishehtadbir@yahoo.com
authors.forename: Marzieh
authors.initials: M
authors.lastname: Hamzavi
authors.forename: Azadeh Andisheh
authors.initials: AA
authors.lastname: Tadbir
authors.forename: Gita
authors.initials: G
authors.lastname: Rezvani
authors.forename: Mohammad Javad
authors.initials: MJ
authors.lastname: Ashraf
authors.forename: Mohammad Javad
authors.initials: MJ
authors.lastname: Fattahi
authors.forename: Bijan
authors.initials: B
authors.lastname: Khademi
authors.forename: Yasaman
authors.initials: Y
authors.lastname: Sardari
authors.forename: Naghmeh
authors.initials: N
authors.lastname: Jeirudi
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism*; D001798:Blood Proteins / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005500:Follow-Up Studies; D006258:Head and Neck Neoplasms / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D007124:Immunoenzyme Techniques; D016753:Interleukin-10 / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D011379:Prognosis; D012463:Saliva / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D001798:Blood Proteins; D016753:Interleukin-10