title: Attachable solvatochromic fluorophores and bioconjugation studies.
abstract: The synthesis and utility of attachable cyclopenta[b]naphthalene solvatochromic fluorophores related to Prodan are described. Two fluorophores were selected for functionalization and bioconjugation studies. The skeletons were chemically modified to include reactive functional groups and showed minimal alteration of the optical properties when compared to the parent dyes. The functionalized fluorophores were covalently attached to the carboxyl group of a fatty acid, and azido- and thiol-containing amino acids, demonstrating their potential for labeling biomolecules.
authors.affiliation: Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA.
authors.forename: Erica
authors.initials: E
authors.lastname: Benedetti
authors.forename: Andrea B E
authors.initials: AB
authors.lastname: Veliz
authors.forename: Mélanie
authors.initials: M
authors.lastname: Charpenay
authors.forename: Laura S
authors.initials: LS
authors.lastname: Kocsis
authors.forename: Kay M
authors.initials: KM
authors.lastname: Brummond
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D003517:Cyclopentanes / Q000138:chemical synthesis* / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D015394:Molecular Structure; D009281:Naphthalenes / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D003517:Cyclopentanes; D005227:Fatty Acids; D005456:Fluorescent Dyes; D009281:Naphthalenes; D012997:Solvents; D013438:Sulfhydryl Compounds; C031721:naphthalene