title: Preparation of primary cultured dopaminergic neurons from mouse brain.
abstract: Dopaminergic neurons are involved in a variety of normal brain functions; degenerations of these neurons cause diseases in human. Investigation of how dopaminergic neurons respond to extracellular signals and molecular mechanisms regulating dopaminergic neuron survival and death often requires reliable, reproducible, and high-quality primary cultures of dopaminergic neurons. Here, we described methods to dissect and culture these neurons from embryonic mesencephalon of mouse brain. We utilize coverslips made from Aclar film to maximize the number of surviving dopaminergic neuron in the culture and immunocytochemistry of tyrosine hydroxylase (TH) to identify dopaminergic neuron.
authors.affiliation: School of Biological Sciences and Technology, College of Natural Sciences, College of Medicine, Chonnam National University, Gwangju, South Korea.
authors.forename: Won-Seok
authors.initials: WS
authors.lastname: Choi
authors.forename: Hyung-Wook
authors.initials: HW
authors.lastname: Kim
authors.forename: Zhengui
authors.initials: Z
authors.lastname: Xia
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology*; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D059290:Dopaminergic Neurons / Q000166:cytology*; D004622:Embryo, Mammalian / Q000166:cytology; D006801:Humans; D007150:Immunohistochemistry; D008636:Mesencephalon / Q000166:cytology / Q000196:embryology; D051379:Mice; D051381:Rats; D013194:Staining and Labeling; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D014446:Tyrosine 3-Monooxygenase