title: Cultivable actinomycetes from rhizosphere of birch (Betula pendula) growing on a coal mine dump in Silets, Ukraine.
abstract: Five actinomycete strains were isolated from the rhizosphere of birch, one of a few native tree forms capable of thriving on the upper level of a coal mine dump near the village of Silets (Lvivska region, Ukraine). No such strains were isolated from surrounding gangue, or from nearby grass Calamagrostis epigeios. Using 16S rDNA sequencing and analysis of cell wall aminoacids, four of these strains were shown to belong to genus Streptomyces and one to be Amycolatopsis. The isolates were able to produce siderophores and antibacterial compounds. In comparison to the reference strain Streptomyces coelicolor M145, certain rhizospheric isolates displayed somewhat increased survival in the presence of copper, iron(III), or chromium(VI) salts. The Amycolatopsis isolate was also shown to accumulate significant quantities of heavy metals from waste extracts. Possible roles of the described strains in coal mine dump ecology are discussed.
authors.affiliation: Department of Genetics and Biotechnology, Ivan Franko National University of Lviv, Lviv, Ukraine.
authors.forename: Bohdan
authors.initials: B
authors.lastname: Ostash
authors.forename: Tetiana
authors.initials: T
authors.lastname: Gren
authors.forename: Yaroslav
authors.initials: Y
authors.lastname: Hrubskyy
authors.forename: Stepan
authors.initials: S
authors.lastname: Tistechok
authors.forename: Stepan
authors.initials: S
authors.lastname: Beshley
authors.forename: Volodymyr
authors.initials: V
authors.lastname: Baranov
authors.forename: Victor
authors.initials: V
authors.lastname: Fedorenko
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D001483:Base Sequence; D029662:Betula / Q000382:microbiology*; D002473:Cell Wall / Q000378:metabolism; D003032:Coal Mining; D004275:DNA, Ribosomal / Q000235:genetics; D019216:Metals, Heavy / Q000378:metabolism; D016533:Mycological Typing Techniques; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D017262:Siderophores / Q000096:biosynthesis; D012988:Soil Microbiology; D013302:Streptomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014455:Ukraine
chemical_list: D000900:Anti-Bacterial Agents; D004275:DNA, Ribosomal; D019216:Metals, Heavy; D012336:RNA, Ribosomal, 16S; D017262:Siderophores
keywords: 16S rRNA; Actinomycetes; Mine tailings; Rhizosphere