title: Comparison of allogeneic platelet lysate and fetal bovine serum for in vitro expansion of equine bone marrow-derived mesenchymal stem cells.
abstract: Mesenchymal stem cells (MSCs) are promising candidates for cell-based therapy and tissue engineering approaches. Fetal bovine serum (FBS) is commonly used for in vitro MSC expansion; however, the use of FBS may be associated with ethical, scientific, and safety issues. This study aimed to compare the ability of allogeneic platelet lysate (PL) and FBS to cause equine bone marrow-derived MSC expansion. MSCs were isolated from bone marrow aspirate in media supplemented with either PL or FBS, and cell proliferation properties and characteristics were examined. There were no significant differences in MSC yield, colony-forming unit-fibroblast (CFU-F) assay, and population doubling time between PL and FBS cultures. In addition, both PL-MSCs and FBS-MSCs showed similar results in term of ALP staining, osteogenic differentiation, and RT-PCR, although there were subtle differences in morphology, growth pattern, and adhesive properties. These results suggest that PL is a suitable alternative to FBS for use in equine MSC expansion, without the problems related to FBS use.
authors.affiliation: Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Jong-pil
authors.initials: JP
authors.lastname: Seo
authors.forename: Nao
authors.initials: N
authors.lastname: Tsuzuki
authors.forename: Shingo
authors.initials: S
authors.lastname: Haneda
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Yamada
authors.forename: Hidefumi
authors.initials: H
authors.lastname: Furuoka
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Tabata
authors.forename: Naoki
authors.initials: N
authors.lastname: Sasaki
mesh_terms: D000469:Alkaline Phosphatase; D000818:Animals; D001792:Blood Platelets; D002417:Cattle; D018929:Cell Culture Techniques; D002457:Cell Extracts / Q000494:pharmacology*; D049109:Cell Proliferation; D005312:Fetal Blood; D006736:Horses / Q000502:physiology*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D010006:Osteoblasts; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013194:Staining and Labeling
chemical_list: D002457:Cell Extracts; D000469:Alkaline Phosphatase
keywords: Fetal bovine serum; Horse; Mesenchymal stem cell; Platelet lysate; Platelet rich plasma