title: Identification and characterization of cyclic nucleotide phosphodiesterases.
abstract: Cyclic nucleotide phosphodiesterases regulate cellular levels of small molecule second messengers that control important biological processes in all kingdoms of life. Identifying and characterizing these enzymes is necessary for basic research and pharmaceutical applications. Here, we describe the use of thin layer chromatography to analyze cellular extracts or purified proteins for cyclic nucleotide phosphodiesterase activity.
authors.affiliation: Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
authors.forename: Erin B
authors.initials: EB
authors.lastname: Purcell
authors.forename: Rita
authors.initials: R
authors.lastname: Tamayo
mesh_terms: D015105:3',5'-Cyclic-AMP Phosphodiesterases / Q000378:metabolism*; D015106:3',5'-Cyclic-GMP Phosphodiesterases / Q000378:metabolism*; D001345:Autoradiography; D002482:Cellulose; D002855:Chromatography, Thin Layer / Q000379:methods*; D057075:Enzyme Assays; D009712:Nucleotides, Cyclic / Q000378:metabolism; D010761:Phosphorus Radioisotopes; D013997:Time Factors
chemical_list: D009712:Nucleotides, Cyclic; D010761:Phosphorus Radioisotopes; D002482:Cellulose; D015105:3',5'-Cyclic-AMP Phosphodiesterases; D015106:3',5'-Cyclic-GMP Phosphodiesterases