title: Effect of low-level laser therapy on types I and III collagen and inflammatory cells in rats with induced third-degree burns.
abstract: Low-level laser therapy (LLLT) has been increasingly used to accelerate wound healing in third-degree burns. This study investigated the effects of lasers on the tissue repair process of third-degree burns. Burns were produced on the backs of male Wistar rats. The animals were divided into four groups (n = 12): control, injury, LLLT 3 J/cm(2), and LLLT 4 J/cm(2). Each group was further divided into two subgroups; the rats in one subgroup were killed on day 8 and those in the other, on day 16 after injury. The animals in LLLT 3 J/cm(2) and LLLT 4 J/cm(2) were irradiated 1 h after injury, and irradiation was repeated every 48 h. Laser (660 nm, 35 mW) treatment at fluences of 3 and 4 J/cm(2) were used. After killing the rats, tissue fragments from the burnt area were removed for histological analysis. The LLLT-treated groups showed a significant decrease (p <0.05) in the number of inflammatory cells and increased collagen deposition compared to the injury group. Laser irradiation (both 3 and 4 J/cm(2)) resulted in reduction in the inflammatory process and improved collagen deposition, thereby ameliorating the healing of third-degree burns.
authors.affiliation: Health Sciences Center, Chapecó University (UNOCHAPECÓ), Av. Sen. Atílio Fontana, Chapecó, SC, Brazil.
authors.forename: Franciane B
authors.initials: FB
authors.lastname: Fiório
authors.forename: Regiane
authors.initials: R
authors.lastname: Albertini
authors.forename: Ernesto Cesar Pinto
authors.initials: EC
authors.lastname: Leal-Junior
authors.forename: Paulo de Tarso Camillo
authors.initials: Pde T
authors.lastname: de Carvalho
mesh_terms: D000818:Animals; D002056:Burns / Q000378:metabolism* / Q000473:pathology / Q000532:radiotherapy*; D024042:Collagen Type I / Q000378:metabolism*; D024061:Collagen Type III / Q000378:metabolism*; D004195:Disease Models, Animal; D007249:Inflammation / Q000473:pathology / Q000532:radiotherapy; D054023:Lasers, Semiconductor / Q000627:therapeutic use; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D008859:Microscopy, Polarization; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014945:Wound Healing / Q000528:radiation effects
chemical_list: D024042:Collagen Type I; D024061:Collagen Type III