title: Synthesis of poly-(3-hydroxybutyrate-co-12 mol % 3-hydroxyvalerate) by Bacillus cereus FB11: its characterization and application as a drug carrier.
abstract: The synthesis of microbial polyhydroxyalkanoate is investigated in this work for it potential application as drug carrier for cancer therapy. The bacterial isolate Bacillus cereus FB11 has synthesized poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer under nutrient stress conditions using glucose as a sole carbon source. The FTIR spectrum of the purified copolymer showed the characteristic absorption bands at 1,719, 1,260 and 2,931 cm(-1) attributing to C=O, C-O stretching and C-H vibrations, respectively. The result of (1)H-NMR confirmed that it was composed of 88 mol % of 3-hydroxybutyrate and 12 mol % of 3-hydroxyvalerate monomeric subunits. The nanoparticles were fabricated from copolymer and used as a carrier for anticancer drug ellipticine. The in vitro drug release studies showed that % inhibition of A549 cancer cell line receiving ellipticine loaded poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) nanoparticles was two-fold higher in comparison to ellipticine alone. This drug delivery system offers exciting possibilities for cancer therapy by increasing the bioavailability of anti-neoplastic drug to the tumor site.
authors.affiliation: Department of Microbiology, Quaid-i-Azam University (QAU), Islamabad, Pakistan. farha.masood@yahoo.com
authors.forename: Farha
authors.initials: F
authors.lastname: Masood
authors.forename: P
authors.initials: P
authors.lastname: Chen
authors.forename: Tariq
authors.initials: T
authors.lastname: Yasin
authors.forename: Fariha
authors.initials: F
authors.lastname: Hasan
authors.forename: Bashir
authors.initials: B
authors.lastname: Ahmad
authors.forename: Abdul
authors.initials: A
authors.lastname: Hameed
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage*; D001409:Bacillus cereus / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy; D045744:Cell Line, Tumor; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism*; D004611:Ellipticines / Q000008:administration & dosage*; D005285:Fermentation; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy; D053758:Nanoparticles; D010802:Phylogeny; D011091:Polyesters / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D012988:Soil Microbiology; D017550:Spectroscopy, Fourier Transform Infrared; D000094122:Polyhydroxybutyrates
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D004611:Ellipticines; D011091:Polyesters; C052620:poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate); D000094122:Polyhydroxybutyrates; C034192:ellipticine