title: Serum Proteome Changes in Healthy Subjects with Different Genotypes of NOS1AP in the Chinese Population.
abstract: Type 2 diabetes and its chronic complications have become a worldwide epidemic nowadays. However, its molecular mechanism is still unknown. We have previously identified a novel variant rs12742393 of NOS1AP for type 2 diabetes susceptibility in the Chinese population. In this study, we analyzed the total serum profiling among three genotypes of rs12742393 to discover potential crosstalk under the variant and the disease through proteomic analyses for the first time. We used OFFGEL peptide fractionation, LC-MS/MS analysis, and label-free quantification to profile the fasting human serum samples of the genotypes in rs12742393 (n = 4, for CC, AC, and AA, resp.). Four proteins were identified, including apoA4, alpha1-ACT, HABP2, and keratin 10, with blood levels changed significantly between CC and AA homozygotes of rs12742393. Compared with AA group, the levels of apoA4 increased (P = 0.000265), whereas the concentration of alpha1-ACT, HABP2, and keratin 10 decreased in CC group (P = 0.011116, 0.021175, and 0.015661, resp.). Then we selected additional fasting serum samples for ELISA and western blot validation. However, no significant differences were identified by neither ELISA nor western blot (P > 0.05). The protein profiling changes between the genotypes of rs12742393 indicated that this SNP might play a role in the development of type 2 diabetes.
authors.affiliation: Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Clinical Center for Diabetes, 600 Yishan Road, Shanghai 200233, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Jiang
authors.forename: Congrong
authors.initials: C
authors.lastname: Wang
authors.forename: Rongxia
authors.initials: R
authors.lastname: Li
authors.forename: Quanhu
authors.initials: Q
authors.lastname: Sheng
authors.forename: Cheng
authors.initials: C
authors.lastname: Hu
authors.forename: Rong
authors.initials: R
authors.lastname: Zhang
authors.forename: Qichen
authors.initials: Q
authors.lastname: Fang
authors.forename: Yuqian
authors.initials: Y
authors.lastname: Bao
authors.forename: Kunsan
authors.initials: K
authors.lastname: Xiang
authors.forename: Rong
authors.initials: R
authors.lastname: Zeng
authors.forename: Weiping
authors.initials: W
authors.lastname: Jia