title: Routine bacterial analysis with automated flow cytometry.
abstract: The impact of multi-well plate automation on bacterial flow cytometric analyses was investigated. Cell concentrations in up to 96 samples can be measured accurately, as long as a reproducible staining protocol and a total measurement time of below 80 min is used. Fluorescence distribution in the samples may, however, display some variability.
authors.affiliation: Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, B-9000 Gent, Belgium.
authors.forename: Sam
authors.initials: S
authors.lastname: Van Nevel
authors.affiliation: Eawag, Swiss Federal Institute of Aquatic Science and Technology, CH-8600 Dübendorf, Switzerland.
authors.forename: Stefan
authors.initials: S
authors.lastname: Koetzsch
authors.affiliation: Eawag, Swiss Federal Institute of Aquatic Science and Technology, CH-8600 Dübendorf, Switzerland.
authors.forename: Hans-Ulrich
authors.initials: HU
authors.lastname: Weilenmann
authors.affiliation: Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Coupure Links 653, B-9000 Gent, Belgium.
authors.forename: Nico
authors.initials: N
authors.lastname: Boon
authors.affiliation: Eawag, Swiss Federal Institute of Aquatic Science and Technology, CH-8600 Dübendorf, Switzerland. Electronic address: frederik.hammes@eawag.ch.
authors.forename: Frederik
authors.initials: F
authors.lastname: Hammes
mesh_terms: D001331:Automation / Q000295:instrumentation / Q000379:methods*; D001419:Bacteria / Q000737:chemistry / Q000166:cytology*; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods*; D005453:Fluorescence
keywords: Automation; Flow cytometry; High throughput analysis