title: Effects of disintegration on in vitro fermentation and conversion patterns of wheat aleurone in a metabolical colon model.
abstract: This work aimed to elucidate the effect of wheat aleurone integrity on its fermentability, i.e., the formation of short-chain fatty acids (SCFA) and microbial phenolic metabolites, in an in vitro model using human faecal microbiota as an inoculum. The structure of aleurone was modified by mechanical (dry grinding) or enzymatic (xylanase with or without feruloyl esterase) treatments in order to increase its physical accessibility and degrade its complex cell-wall network. The ground aleurone (smaller particle size) produced slightly more SCFA than the native aleurone during the first 8 h but a similar amount at 24 h (102.5 and 101 mmol/L, respectively). Similar colonic metabolism of ferulic acid (FA) was observed for native and ground aleurone. The enzymatic treatments of aleurone allowed a high solubilization of arabinoxylan (up to 82%) and a high release of FA in its conjugated and free forms (up to 87%). The enzymatic disintegration of aleurone's structure led to a higher concentration and formation rate of the colonic metabolites of FA (especially phenylpropionic acids) but did not change significantly the formation of SCFA (81 mmol/L for enzyme treated versus 101 mmol/L for the native aleurone).
authors.affiliation: Montpellier SupAgro-INRA-UMII-CIRAD , JRU1208 Agropolymers Engineering and Emerging Technologies, 2 place Pierre Viala, F-34060 Montpellier, France.
authors.forename: Natalia N
authors.initials: NN
authors.lastname: Rosa
authors.forename: Anna-Marja
authors.initials: AM
authors.lastname: Aura
authors.forename: Luc
authors.initials: L
authors.lastname: Saulnier
authors.forename: Ulla
authors.initials: U
authors.lastname: Holopainen-Mantila
authors.forename: Kaisa
authors.initials: K
authors.lastname: Poutanen
authors.forename: Valérie
authors.initials: V
authors.lastname: Micard
mesh_terms: D002265:Carboxylic Ester Hydrolases / Q000378:metabolism; D003106:Colon / Q000378:metabolism / Q000382:microbiology*; D004040:Dietary Carbohydrates / Q000032:analysis / Q000378:metabolism*; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism; D056625:Endosperm / Q000737:chemistry* / Q000166:cytology; D005232:Fatty Acids, Volatile / Q000378:metabolism; D005243:Feces / Q000382:microbiology; D005285:Fermentation; D005387:Finland; D005511:Food Handling; D005656:Fungal Proteins / Q000378:metabolism; D006090:Gram-Negative Bacteria / Q000302:isolation & purification / Q000378:metabolism; D006094:Gram-Positive Bacteria / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism / Q000382:microbiology*; D008954:Models, Biological; D010316:Particle Size; D010636:Phenols / Q000378:metabolism; D014908:Triticum / Q000737:chemistry* / Q000166:cytology
chemical_list: D004040:Dietary Carbohydrates; D005232:Fatty Acids, Volatile; D005656:Fungal Proteins; D010636:Phenols; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase; D043364:Endo-1,4-beta Xylanases