title: Using genetically encoded fluorescent reporters to image lipid signalling in living plants.
abstract: The discovery of the green fluorescent protein has revolutionized cell biology as it allowed researchers to visualize dynamic processes in living cells. The fusion of fluorescent protein variants with lipid binding domains that bind to specific phospholipids have been very instrumental in investigating the role of these molecules in living plants. Here, we describe the use of these reporters to image lipids in living Arabidopsis seedlings using fluorescence microscopy.
authors.affiliation: Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland.
authors.forename: Joop E M
authors.initials: JE
authors.lastname: Vermeer
authors.forename: Teun
authors.initials: T
authors.lastname: Munnik
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D017930:Genes, Reporter / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics*; D050356:Lipid Metabolism; D018613:Microscopy, Confocal; D057054:Molecular Imaging / Q000379:methods*; D036226:Seedlings; D015398:Signal Transduction
chemical_list: D049452:Green Fluorescent Proteins