title: Highly sensitive fluorescent detection of small molecules, ions, and proteins using a universal label-free aptasensor.
abstract: A facile and universal aptamer-based label-free approach for highly selective and sensitive fluorescence detection of a broad range of targets including small molecules, inorganic ions and proteins was developed by using PicoGreen to transduce the fluorescent signal of the double stranded DNA duplex formed between a free aptamer and its complementary strand.
authors.affiliation: Institute of Quality Standards and Testing Technology for Agro-products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences, Beijing, 100081, P.R. China.
authors.forename: Zhenzhen
authors.initials: Z
authors.lastname: Lv
authors.forename: Jinchuan
authors.initials: J
authors.lastname: Liu
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhou
authors.forename: Zheng
authors.initials: Z
authors.lastname: Guan
authors.forename: Shuming
authors.initials: S
authors.lastname: Yang
authors.forename: Cheng
authors.initials: C
authors.lastname: Li
authors.forename: Ailiang
authors.initials: A
authors.lastname: Chen
mesh_terms: D000241:Adenosine / Q000032:analysis; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques; D007477:Ions / Q000032:analysis*; D009930:Organic Chemicals / Q000737:chemistry; D011188:Potassium / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D013050:Spectrometry, Fluorescence; D013917:Thrombin / Q000032:analysis
chemical_list: D052157:Aptamers, Nucleotide; D007477:Ions; D009930:Organic Chemicals; C099476:PicoGreen; D011506:Proteins; D013917:Thrombin; D000241:Adenosine; D011188:Potassium