title: Real time PCR detection of rabbit haemorrhagic disease virus in rabbits infected with different European strains of RHDV.
abstract: The paper concerns the use of a novel, very effective diagnostic method, a real-time PCR for diagnosis of a viral agent causing viral haemorrhagic disease in rabbits - RHDV. Until now, the method was widely used for detecting many different viruses, both DNA, and RNA, but as far as RHDV is concerned, there are not many records of such use. This study aimed at the detection of 17 different strains from different European regions, differing in biological features and mortality. The study confirmed that real-time PCR is an applicable and effective method for diagnosis of RHDV, irrespective of the stains' features.
authors.affiliation: Department of Immunology, Faculty of Biology, University of Szczecin, Felczaka 3c, 71-412 Szczecin. paulina.niedzwiedzka@gmail.com
authors.forename: P
authors.initials: P
authors.lastname: Niedźwiedzka-Rystwej
authors.forename: B
authors.initials: B
authors.lastname: Hukowska-Szematowicz
authors.forename: J
authors.initials: J
authors.lastname: Działo
authors.forename: B
authors.initials: B
authors.lastname: Tokarz-Deptuła
authors.forename: W
authors.initials: W
authors.lastname: Deptuła
mesh_terms: D000818:Animals; D017250:Caliciviridae Infections / Q000175:diagnosis / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D005060:Europe / Q000453:epidemiology; D017929:Hemorrhagic Disease Virus, Rabbit / Q000145:classification* / Q000302:isolation & purification*; D011817:Rabbits; D060888:Real-Time Polymerase Chain Reaction / Q000662:veterinary*