title: Character comparison of abdomen-derived and eyelid-derived mesenchymal stem cells.
abstract: OBJECTIVE
  While most human adipose tissues, such as those located in the abdomen, hip and thigh, are of mesodermal origin, adipose tissues located in the face are of ectodermal origin. The present study has compared stem cell-related features of abdomen-derived adult stem cells (A-ASCs) with those of eyelid-derived adult stem cells (E-ASCs).
  
  
  METHODS
  Adipose tissue-derived cells were maintained in DMEM supplemented with 10% FBS. Before passage 6, cells were analysed using FACS, immunocytochemistry and quantitative real time PCR (qRT-PCR). To examine multi-differentiational potential, early passage ASCs were cultivated in each of a commercial Stempro(®) Differentiation kit.
  
  
  RESULTS
  Unlike fibroblast-like morphology of A-ASCs, E-ASCs had bipolar morphology. Both types of cell exhibited similar surface antigens, and neuronal cell-related genes and proteins. However, there were differences in mRNA expression levels of CD90 and CD146; neuron-specific enolase (NSE) and nuclear receptor-related protein 1 (Nurr1) were different between the two cell types. There was no difference in multi-differentiational potential between 3 E-ASCs lines, however, E-ASCs had higher expression levels of chondrocyte-related genes compared to A-ASCs. These cells underwent senescence and maintained normal karyotypes.
  
  
  CONCLUSIONS
  Although isolated from similar adipose tissues, both types of cells displayed many contrasting characteristics. Understanding defining phenotypes of such cells is useful for making suitable choices in differing clinical indications.
authors.affiliation: National Institute of Food and Drug Safety Evaluation, Korea Food and Drug Administration, Cheongwon-gun, Chuncheongbuk-do 363-700, South Korea.
authors.forename: S-H
authors.initials: SH
authors.lastname: Kim
authors.forename: S H
authors.initials: SH
authors.lastname: Bang
authors.forename: S A
authors.initials: SA
authors.lastname: Park
authors.forename: S Y
authors.initials: SY
authors.lastname: Kang
authors.forename: K D
authors.initials: KD
authors.lastname: Park
authors.forename: I U
authors.initials: IU
authors.lastname: Oh
authors.forename: S H
authors.initials: SH
authors.lastname: Yoo
authors.forename: H
authors.initials: H
authors.lastname: Kim
authors.forename: C-H
authors.initials: CH
authors.lastname: Kim
authors.forename: S Y
authors.initials: SY
authors.lastname: Baek
mesh_terms: D000005:Abdomen; D017667:Adipocytes / Q000166:cytology*; D000273:Adipose Tissue / Q000166:cytology*; D053687:Adult Stem Cells / Q000378:metabolism*; D000954:Antigens, Surface / Q000378:metabolism; D051929:CD146 Antigen / Q000235:genetics; D002454:Cell Differentiation; D002478:Cells, Cultured; D005143:Eyelids / Q000166:cytology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D012333:RNA, Messenger; D018800:Thy-1 Antigens / Q000235:genetics
chemical_list: D000954:Antigens, Surface; D051929:CD146 Antigen; D012333:RNA, Messenger; D018800:Thy-1 Antigens