title: Inactive or moderately active human promoters are enriched for inter-individual epialleles.
abstract: BACKGROUND
  Inter-individual epigenetic variation, due to genetic, environmental or random influences, is observed in many eukaryotic species. In mammals, however, the molecular nature of epiallelic variation has been poorly defined, partly due to the restricted focus on DNA methylation. Here we report the first genome-scale investigation of mammalian epialleles that integrates genomic, methylomic, transcriptomic and histone state information.
  
  
  RESULTS
  First, in a small sample set, we demonstrate that non-genetically determined inter-individual differentially methylated regions (iiDMRs) can be temporally stable over at least 2 years. Then, we show that iiDMRs are associated with changes in chromatin state as measured by inter-individual differences in histone variant H2A.Z levels. However, the correlation of promoter iiDMRs with gene expression is negligible and not improved by integrating H2A.Z information. We find that most promoter epialleles, whether genetically or non-genetically determined, are associated with low levels of transcriptional activity, depleted for housekeeping genes, and either depleted for H3K4me3/enriched for H3K27me3 or lacking both these marks in human embryonic stem cells. The preferential enrichment of iiDMRs at regions of relative transcriptional inactivity validates in a larger independent cohort, and is reminiscent of observations previously made for promoters that undergo hypermethylation in various cancers, in vitro cell culture and ageing.
  
  
  CONCLUSIONS
  Our work identifies potential key features of epiallelic variation in humans, including temporal stability of non-genetically determined epialleles, and concomitant perturbations of chromatin state. Furthermore, our work suggests a novel mechanistic link among inter-individual epialleles observed in the context of normal variation, cancer and ageing.
authors.forename: Carolina
authors.initials: C
authors.lastname: Gemma
authors.forename: Sreeram V
authors.initials: SV
authors.lastname: Ramagopalan
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Down
authors.forename: Huriya
authors.initials: H
authors.lastname: Beyan
authors.forename: Mohammed I
authors.initials: MI
authors.lastname: Hawa
authors.forename: Michelle L
authors.initials: ML
authors.lastname: Holland
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Hurd
authors.forename: Gavin
authors.initials: G
authors.lastname: Giovannoni
authors.forename: R David
authors.initials: RD
authors.lastname: Leslie
authors.forename: George C
authors.initials: GC
authors.lastname: Ebers
authors.forename: Vardhman K
authors.initials: VK
authors.lastname: Rakyan
mesh_terms: D000483:Alleles; D019175:DNA Methylation; D053595:Embryonic Stem Cells / Q000378:metabolism*; D044127:Epigenesis, Genetic; D005260:Female; D005786:Gene Expression Regulation; D015894:Genome, Human; D023281:Genomics / Q000379:methods*; D006657:Histones / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D014430:Twins, Monozygotic / Q000235:genetics*
chemical_list: D006657:Histones