title: Nitric oxide levels in cochlear fluids of guinea pig.
abstract: OBJECTIVE
  To describe a technique to obtain guinea pigs cochlear fluids and measure nitric oxide (NO) concentration.
  
  
  METHODS
  Six guinea pigs were used and sacrificed. The cochlear fluids collected for measurement of NO, performed by chemiluminescence (NOA 280).
  
  
  RESULTS
  Through the chemiluminescence was possible to analyze the concentration of NO in cochlear fluids obtained. Average levels of nitric oxide from guinea pigs was 12.55 µM.
  
  
  CONCLUSIONS
  It is possible to obtain nitric oxide cochlear fluids, with this technique and nitric oxide concentration measure by chemiluminescence, a quantitative and more precise method.
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Federal University of Sao Paulo, Brazil. ferky@uol.com.br
authors.forename: Fernando Kaoru
authors.initials: FK
authors.lastname: Yonamine
authors.forename: Ektor Tsuneo
authors.initials: ET
authors.lastname: Onishi
authors.forename: Margareth Gori
authors.initials: MG
authors.lastname: Mouro
authors.forename: Elisa Mieko Suemitsu
authors.initials: EM
authors.lastname: Higa
authors.forename: Oswaldo Laércio Mendonça
authors.initials: OL
authors.lastname: Cruz
mesh_terms: D000818:Animals; D003051:Cochlea / Q000378:metabolism* / Q000601:surgery; D006168:Guinea Pigs; D049449:Luminescence; D008297:Male; D023421:Models, Animal; D009569:Nitric Oxide / Q000032:analysis*; D019001:Nitric Oxide Synthase / Q000032:analysis; D015203:Reproducibility of Results
chemical_list: D009569:Nitric Oxide; D019001:Nitric Oxide Synthase