title: [SCoT differential expression of cold resistance related genes in Dendrobium officinale under low temperature stress].
abstract: In order to study the molecule mechanism of the differential expression in Dendrobium officinale under low temperature, the high cold resistance germplasms were used for constructing the RNA pools. SCoT markers were used to analyze the different cDNA pools transcribed from the RNA pools. 11 transcripts derived fragments from 500 cDNA amplified bands were amplified by 64 primers, and were sorted out, cloned, sequenced and analyzed. The results showed that cDNA pools with SCoT markers could be used for differential display in D. officinale under low temperature stress. Sequence analysis indicated that the transcripts derived fragments were significantly homologous in nucleotide sequence with membrane-associated proteins, osmotic regulation protein, CBF transcriptional factor, resistance protein. One left gene segments functions were still unknown, which may be related to the cold resistant gene expression in D. officinale.
authors.affiliation: Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang Provinical Strategic Allance for Technical Innovation in Industry of Dendrobium officinale Zhejiang Agricultural and Forestry University, Lin'an 311300, China. comeonldb@163.com
authors.forename: Dong-Bin
authors.initials: DB
authors.lastname: Li
authors.forename: Yan-Hui
authors.initials: YH
authors.lastname: Gao
authors.forename: Jin-Ping
authors.initials: JP
authors.lastname: Si
mesh_terms: D001947:Breeding; D003080:Cold Temperature / Q000009:adverse effects*; D031667:Dendrobium / Q000235:genetics* / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D005819:Genetic Markers / Q000235:genetics; D013312:Stress, Physiological / Q000235:genetics*
chemical_list: D005819:Genetic Markers