title: Endocrine and metabolic signaling in retroperitoneal white adipose tissue remodeling during cold acclimation.
abstract: The expression profiles of adiponectin, resistin, 5'-AMP-activated protein kinase α (AMPK α ), hypoxia-inducible factor-1 α (HIF-1 α ), and key enzymes of glucose and fatty acid metabolism and oxidative phosphorylation in rat retroperitoneal white adipose tissue (RpWAT) during 45-day cold acclimation were examined. After transient suppression on day 1, adiponectin protein level increased following sustained cold exposure. In parallel, on day 1, the protein level of HIF-1 α was strongly induced and AMPK α suppressed, while afterwards the reverse was seen. What is more, after an initial decrease on day 1, a sequential increase in pyruvate dehydrogenase, acyl-CoA dehydrogenase, cytochrome c oxidase, and ATP synthase and a decrease in acetyl-CoA carboxylase (from day 3) were observed. Similar to adiponectin, protein level of resistin showed a biphasic profile: it increased after days 1, 3, and 7 and decreased below the control after 21 days of cold-acclimation. In summary, the data suggest that adiponectin and resistin are important integrators of RpWAT metabolic response and roles it plays during cold acclimation. It seems that AMPK α mediate adiponectin effects on metabolic remodeling RpWAT during cold acclimation.
authors.affiliation: Department of Physiology, Institute for Biological Research "Sinisa Stankovic", University of Belgrade, 11060 Belgrade, Serbia.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Jankovic
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Korac
authors.forename: Biljana
authors.initials: B
authors.lastname: Buzadzic
authors.forename: Vesna
authors.initials: V
authors.lastname: Otasevic
authors.forename: Ana
authors.initials: A
authors.lastname: Stancic
authors.forename: Milica
authors.initials: M
authors.lastname: Vucetic
authors.forename: Milica
authors.initials: M
authors.lastname: Markelic
authors.forename: Ksenija
authors.initials: K
authors.lastname: Velickovic
authors.forename: Igor
authors.initials: I
authors.lastname: Golic
authors.forename: Bato
authors.initials: B
authors.lastname: Korac
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D025181:ATP Synthetase Complexes / Q000378:metabolism; D000064:Acclimatization; D000103:Acetyl-CoA Carboxylase / Q000378:metabolism; D042964:Acyl-CoA Dehydrogenase / Q000378:metabolism; D052242:Adiponectin / Q000378:metabolism; D000818:Animals; D001833:Body Temperature Regulation; D003080:Cold Temperature; D003576:Electron Transport Complex IV / Q000378:metabolism; D004734:Energy Metabolism; D005227:Fatty Acids / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism; D050152:Intra-Abdominal Fat / Q000378:metabolism*; D008297:Male; D010085:Oxidative Phosphorylation; D010766:Phosphorylation; D030481:Pyruvate Dehydrogenase (Lipoamide) / Q000378:metabolism; D051381:Rats; D052243:Resistin / Q000378:metabolism; D015398:Signal Transduction; D013997:Time Factors
chemical_list: D052242:Adiponectin; C499920:Adipoq protein, rat; D005227:Fatty Acids; C497444:Hif1a protein, rat; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D052243:Resistin; C499622:Retn protein, rat; D030481:Pyruvate Dehydrogenase (Lipoamide); D042964:Acyl-CoA Dehydrogenase; D003576:Electron Transport Complex IV; D055372:AMP-Activated Protein Kinases; D025181:ATP Synthetase Complexes; D000103:Acetyl-CoA Carboxylase; D005947:Glucose