title: Protective effect of luteolin on an oxidative-stress model induced by microinjection of sodium nitroprusside in mice.
abstract: Accumulating lines of evidence showed that luteolin, a polyphenolic compound, has potent neuroprotective effects. The purpose of this study was to examine whether luteolin can protect against sodium nitroprusside (SNP)-induced oxidative damage in mouse brain. Intrastriatal co-injection of luteolin (3 - 30 nmol) with SNP (10 nmol) dose-dependently protected against brain damage and motor dysfunction. Oral administrations of luteolin (600 - 1200 mg/kg) dose-dependently protected against brain damage and motor dysfunction induced by striatal injection of SNP. Furthermore, luteolin (30 - 100 μM) concentration dependently protected against Fe(2+)-induced lipid peroxidation in mouse brain homogenate. Luteolin (1 - 100 μg/ml) showed potent DPPH radical scavenging ability, when compared with ascorbic acid and glutathione. Finally, a ferrozine assay showed that luteolin (30 - 100 μg/ml) has Fe(2+)-chelating ability, but this was weaker than that of ethylenediaminetetraacetic acid. These results suggest that intrastriatal or oral administration of luteolin protected mice brain from SNP-induced oxidative damage by scavenging and chelating effects.
authors.affiliation: Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan.
authors.forename: Qand Agha
authors.initials: QA
authors.lastname: Nazari
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Kume
authors.forename: Yuki
authors.initials: Y
authors.lastname: Takada-Takatori
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Izumi
authors.forename: Akinori
authors.initials: A
authors.lastname: Akaike
mesh_terms: D060433:Administration, Ophthalmic; D000818:Animals; D000975:Antioxidants; D001925:Brain Damage, Chronic / Q000139:chemically induced* / Q000517:prevention & control*; D003342:Corpus Striatum; D004195:Disease Models, Animal; D016166:Free Radical Scavengers; D007502:Iron Chelating Agents; D047311:Luteolin / Q000008:administration & dosage / Q000494:pharmacology*; D051379:Mice; D008813:Mice, Inbred ICR; D008845:Microinjections; D009043:Motor Activity / Q000187:drug effects; D018696:Neuroprotective Agents; D009599:Nitroprusside / Q000008:administration & dosage* / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D007502:Iron Chelating Agents; D018696:Neuroprotective Agents; D009599:Nitroprusside; D047311:Luteolin