title: Anti-metastatic activity of biologically synthesized gold nanoparticles on human fibrosarcoma cell line HT-1080.
abstract: Plants are exploited as a potential source for the large-scale production of noble gold nanoparticles in the recent years owing to their various potential applications in nanobiotechnology and nanomedicine. The present work describes green biosynthetic procedures for the production of gold nanoparticles for the first time by using an aqueous extract of the Dysosma pleiantha rhizome. The biosynthesized gold nanoparticles were confirmed and characterized by ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy, transmission electron microscopy, and scanning electron microscopy equipped with energy dispersive spectroscopy. The results revealed that aqueous extract of D. pleiantha rhizome has potential to reduce chloroauric ions into gold nanoparticles and the synthesized gold nanoparticles were showed spherical in shape with an average of 127nm. Further, we investigated the anti-metastatic activity of biosynthesized gold nanoparticles against human fibrosarcoma cancer cell line HT-1080. The results showed that the biosynthesized gold nanoparticles were non-toxic to cell proliferation and, also it can inhibit the chemo-attractant cell migration of human fibrosarcoma cancer cell line HT-1080 by interfering the actin polymerization pathway. Thus, the usage of gold nanoparticles biosynthesized from D. pleiantha rhizome can be used as a potential candidate in the drug and gene delivery to metastatic cancer.
authors.affiliation: Department of Applied Chemistry, Chaoyang University of Technology, Taichung 41349, Taiwan.
authors.forename: Palaniyandi
authors.initials: P
authors.lastname: Karuppaiya
authors.forename: Elumalai
authors.initials: E
authors.lastname: Satheeshkumar
authors.forename: Wei-Ting
authors.initials: WT
authors.lastname: Chao
authors.forename: Lin-Yi
authors.initials: LY
authors.lastname: Kao
authors.forename: Emily Chin-Fun
authors.initials: EC
authors.lastname: Chen
authors.forename: Hsin-Sheng
authors.initials: HS
authors.lastname: Tsay
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D005354:Fibrosarcoma / Q000188:drug therapy* / Q000473:pathology; D006046:Gold / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry* / Q000378:metabolism; D010316:Particle Size; D010936:Plant Extracts / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology; D027343:Rhizome / Q000737:chemistry* / Q000378:metabolism; D013329:Structure-Activity Relationship; D013499:Surface Properties; D014407:Tumor Cells, Cultured
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D006046:Gold