title: Identification of cellular proteins interacting with octaarginine (R8) cell-penetrating peptide by photo-crosslinking.
abstract: Octaarginine (R8) is a representative cell-penetrating peptide. Lanthionine synthetase component C-like protein 1 (LanCL1) was identified as a potential intracellular target of R8 by using a photo-crosslinking assay that utilized a phenyl-trifluoromethyl diazirine moiety and peptide mass fingerprinting. Increased cellular uptake of R8 by LanCL1-overexpressing cells was observed.
authors.affiliation: Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan.
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Kawaguchi
authors.forename: Gen
authors.initials: G
authors.lastname: Tanaka
authors.forename: Ikuhiko
authors.initials: I
authors.lastname: Nakase
authors.forename: Miki
authors.initials: M
authors.lastname: Imanishi
authors.forename: Junya
authors.initials: J
authors.lastname: Chiba
authors.forename: Yasumaru
authors.initials: Y
authors.lastname: Hatanaka
authors.forename: Shiroh
authors.initials: S
authors.lastname: Futaki
mesh_terms: D003432:Cross-Linking Reagents / Q000737:chemistry*; D057809:HEK293 Cells; D006801:Humans; D015394:Molecular Structure; D009842:Oligopeptides / Q000737:chemistry*; D055668:Photochemical Processes; D043562:Receptors, G-Protein-Coupled / Q000096:biosynthesis / Q000737:chemistry*
chemical_list: D003432:Cross-Linking Reagents; C485881:LANCL1 protein, human; D009842:Oligopeptides; D043562:Receptors, G-Protein-Coupled; C448619:octaarginine