title: Confocal Raman data analysis enables identifying apoptosis of MCF-7 cells caused by anticancer drug paclitaxel.
abstract: Confocal Raman microscopy is a noninvasive, label-free imaging technique used to study apoptosis of live MCF-7 cells. The images are based on Raman spectra of cells components, and their apoptosis is monitored through diffusion of cytochrome c in cytoplasm. K-mean clustering is used to identify mitochondria in cells, and correlation analysis provides the cytochrome c distribution inside the cells. Our results demonstrate that incubation of cells for 3 h with 10 μM of paclitaxel does not induce apoptosis in MCF-7 cells. On the contrary, incubation for 30 min at a higher concentration (100 μM) of paclitaxel induces gradual release of the cytochrome c into the cytoplasm, indicating cell apoptosis via a caspase independent pathway.
authors.affiliation: Université Montpellier 1, Laboratoire Biologie-Santé Nanosciences, EA 4203, UFR Odontologie, 34193 Montpellier, France. s_hamideh@yahoo.com
authors.forename: Hamideh
authors.initials: H
authors.lastname: Salehi
authors.forename: Elodie
authors.initials: E
authors.lastname: Middendorp
authors.forename: Ivan
authors.initials: I
authors.lastname: Panayotov
authors.forename: Pierre-Yves
authors.initials: PY
authors.lastname: Collart Dutilleul
authors.forename: Pierre-Yves Collard
authors.initials: PY
authors.lastname: Dutilleul
authors.forename: Attila-Gergely
authors.initials: AG
authors.lastname: Vegh
authors.forename: Sathish
authors.initials: S
authors.lastname: Ramakrishnan
authors.forename: Csilla
authors.initials: C
authors.lastname: Gergely
authors.forename: Frederic
authors.initials: F
authors.lastname: Cuisinier
mesh_terms: D000465:Algorithms; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D045304:Cytochromes c / Q000737:chemistry / Q000378:metabolism; D003584:Cytological Techniques / Q000379:methods*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D061986:MCF-7 Cells; D018613:Microscopy, Confocal / Q000379:methods*; D017239:Paclitaxel / Q000494:pharmacology*; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D000970:Antineoplastic Agents; D045304:Cytochromes c; D017239:Paclitaxel