title: Small RNA sequencing-microarray analyses in Parkinson leukocytes reveal deep brain stimulation-induced splicing changes that classify brain region transcriptomes.
abstract: MicroRNAs (miRNAs) are key post transcriptional regulators of their multiple target genes. However, the detailed profile of miRNA expression in Parkinson's disease, the second most common neurodegenerative disease worldwide and the first motor disorder has not been charted yet. Here, we report comprehensive miRNA profiling by next-generation small-RNA sequencing, combined with targets inspection by splice-junction and exon arrays interrogating leukocyte RNA in Parkinson's disease patients before and after deep brain stimulation (DBS) treatment and of matched healthy control volunteers (HC). RNA-Seq analysis identified 254 miRNAs and 79 passenger strand forms as expressed in blood leukocytes, 16 of which were modified in patients pre-treatment as compared to HC. 11 miRNAs were modified following brain stimulation 5 of which were changed inversely to the disease induced changes. Stimulation cessation further induced changes in 11 miRNAs. Transcript isoform abundance analysis yielded 332 changed isoforms in patients compared to HC, which classified brain transcriptomes of 47 PD and control independent microarrays. Functional enrichment analysis highlighted mitochondrion organization. DBS induced 155 splice changes, enriched in ubiquitin homeostasis. Cellular composition analysis revealed immune cell activity pre and post treatment. Overall, 217 disease and 74 treatment alternative isoforms were predictably targeted by modified miRNAs within both 3' and 5' untranslated ends and coding sequence sites. The stimulation-induced network sustained 4 miRNAs and 7 transcripts of the disease network. We believe that the presented dynamic networks provide a novel avenue for identifying disease and treatment-related therapeutic targets. Furthermore, the identification of these networks is a major step forward in the road for understanding the molecular basis for neurological and neurodegenerative diseases and assessment of the impact of brain stimulation on human diseases.
authors.affiliation: Department of Medical Neurobiology, Hadassah Faculty of Medicine, The Hebrew University of Jerusalem Jerusalem, Israel.
authors.forename: Lilach
authors.initials: L
authors.lastname: Soreq
authors.forename: Nathan
authors.initials: N
authors.lastname: Salomonis
authors.forename: Michal
authors.initials: M
authors.lastname: Bronstein
authors.forename: David S
authors.initials: DS
authors.lastname: Greenberg
authors.forename: Zvi
authors.initials: Z
authors.lastname: Israel
authors.forename: Hagai
authors.initials: H
authors.lastname: Bergman
authors.forename: Hermona
authors.initials: H
authors.lastname: Soreq
keywords: Parkinson's disease; deep brain stimulation; high throughput sequencing; leukocytes; miRNAs; splice junction microarrays; substantia nigra