title: [Rapid determination of six preservative residues in fruits and vegetables by high performance liquid chromatography using dispersive solid phase extraction].
abstract: A dispersive solid phase extraction-high performance liquid chromatographic (HPLC) method was established for the simultaneous determination of 2,4-D, thiabendazole, 2-naphthol, o-phenyl phenol, diphenyl ether and biphenyl in the fruits and vegetables. The samples were vortexed for extraction with acetonitrile containing sodium chloride and anhydrous magnesium sulfate. The acetonitrile extraction was purified by acidic alumina dispersed solid phase extraction. The analysis was performed on an Agilent TC C18 column (250 mm x 4.6 mm, 5 microm) and an ultraviolet detector at 235 nm with a mobile phase of methanol-0.02 mol/L (pH 6) potassium dihydrogen phosphate solution, at a flow rate of 1.0 mL/min with gradient elution. Good linearities were observed in the range of 0.5-20 mg/L with correlation coefficients greater than 0.99. The average recoveries of the six preservatives in fruits and vegetables were in the range of 84.2% -99. 1% at three spiked levels of 1, 2 and 10 mg/kg (n = 6) and the relative standard deviations (RSDs) were in the range of 1.67%-10.3%. The limits of quantification were 1 mg/kg. This method is simple, accurate and suitable for the determination of the six preservatives in fruits and vegetables.
authors.affiliation: Inspection and Quarantine Technology Center, Guangdong Enrtry-Exit Inspection and Quarantine Bureau, Guangzhou 510623, China. linhd@iqtc.cn
authors.forename: Haidan
authors.initials: H
authors.lastname: Lin
authors.forename: Zhifei
authors.initials: Z
authors.lastname: Zou
authors.forename: Yan
authors.initials: Y
authors.lastname: Qin
authors.forename: Shouxin
authors.initials: S
authors.lastname: Xie
authors.forename: Yufang
authors.initials: Y
authors.lastname: Chen
authors.forename: Juan
authors.initials: J
authors.lastname: Xu
authors.forename: Lan
authors.initials: L
authors.lastname: Wang
authors.forename: Chuying
authors.initials: C
authors.lastname: Chen
mesh_terms: D001713:Biphenyl Compounds / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D005520:Food Preservatives / Q000032:analysis*; D005638:Fruit / Q000737:chemistry*; D009284:Naphthols / Q000032:analysis; D010636:Phenols / Q000032:analysis; D010647:Phenyl Ethers / Q000032:analysis; D052617:Solid Phase Microextraction / Q000379:methods*; D014675:Vegetables / Q000737:chemistry*
chemical_list: D001713:Biphenyl Compounds; D005520:Food Preservatives; D009284:Naphthols; D010636:Phenols; D010647:Phenyl Ethers; C010574:biphenyl; C031538:phenyl ether; C028405:2-naphthol