title: [An etiological survey on a foodborne disease epidemic outbreak caused by Salmonella enteritidis].
abstract: OBJECTIVE
  To conduct an etiological molecular epidemiological survey and laboratory test on a foodborne disease epidemic outbreak to make clear of the cause and implement effective prevention and control on it.
  
  
  METHODS
  On May 12th 2012, 135 kindergarten children were sent to Xuzhou City People's Hospital and Children's Hospital with gastrointestinal infection disease. A total of 34 anus swab samples and 4 vomit samples were collected from the patients. Real-time PCR rapid detection, strains separation and cultivation, phage lysis experiments, ATB automated identification system were used to make etiological detection and identification. The genomic DNA of salmonella enteritidis were typed with the pulsed-field gel electrophoresis (PFGE), cluster analysis were carried out together with the patterns of local Salmonella infections.
  
  
  RESULTS
  Children in 20 classes were suffered from the gastrointestinal infection among the 21 classes. There were no significant aggregation of class distribution. Among the 135 patients, 76 were boys (56.3%) and 59 were girls (43.7%). The main symptoms were fever (above 38°C), diarrhea and bellyache. Through real-time PCR detection and strains separation, 19 salmonella enteritidis were isolated from 34 anus swab samples of suspected cases and the detection rate was 56%. There were no strains detected from vomit samples. All of the 19 salmonella enteritidis showed the same serological subtype, biochemical reaction, drug sensitivity and phage lysis pattern. The salmonella enteritidis had the identical PFGE pattern (100% similarity), and were different from the pattern of local sporadic infection cases.
  
  
  CONCLUSIONS
  It was confirmed that this was an epidemic outbreak of foodborne disease caused by homologous salmonella enteritidis by epidemiological survey, clinical information, lab etiological test and molecular typing.
authors.affiliation: Department of Clinical Laboratory, Xuzhou Municipal Centre for Disease Control and Prevention, Xuzhou, China.
authors.forename: Jin-chuan
authors.initials: JC
authors.lastname: Yang
authors.forename: Yang
authors.initials: Y
authors.lastname: Xia
authors.forename: Hui
authors.initials: H
authors.lastname: Guo
authors.forename: Jing-jing
authors.initials: JJ
authors.lastname: Xu
authors.forename: Lu-mei
authors.initials: LM
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Tong
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Jun-rong
authors.initials: JR
authors.lastname: Liang
authors.forename: Huai-qi
authors.initials: HQ
authors.lastname: Jing
authors.forename: Zhen-jun
authors.initials: ZJ
authors.lastname: Li
mesh_terms: D001434:Bacteriophage Typing; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D006801:Humans; D008297:Male; D017720:Molecular Epidemiology; D012478:Salmonella Food Poisoning / Q000453:epidemiology* / Q000382:microbiology; D012477:Salmonella enteritidis / Q000145:classification / Q000302:isolation & purification*