title: Substrate specificity of undecaprenyl diphosphate synthase from the hyperthermophilic archaeon Aeropyrum pernix.
abstract: Cis-prenyltransferase from a hyperthermophilic archaeon Aeropyrum pernix was expressed in Escherichia coli and purified for characterization. Properties such as substrate specificity, product chain-length, thermal stability and cofactor requirement were investigated using the recombinant enzyme. In particular, the substrate specificity of the enzyme attracts interest because only dimethylallyl diphosphate and geranylfarnesyl diphosphate, both of which are unusual substrates for known cis-prenyltransferases, are likely available as an allylic primer substrate in A. pernix. From the enzymatic study, the archaeal enzyme was shown to be undecaprenyl diphosphate synthase that has anomalous substrate specificity, which results in a preference for geranylfarnesyl diphosphate. This means that the product of the enzyme, which is probably used as the precursor of the glycosyl carrier lipid, would have an undiscovered structure.
authors.affiliation: Department of Applied Molecular Bioscience, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Mori
authors.forename: Takuya
authors.initials: T
authors.lastname: Ogawa
authors.forename: Tohru
authors.initials: T
authors.lastname: Yoshimura
authors.forename: Hisashi
authors.initials: H
authors.lastname: Hemmi
mesh_terms: D041522:Aeropyrum / Q000201:enzymology* / Q000235:genetics; D019883:Alkyl and Aryl Transferases / Q000235:genetics / Q000378:metabolism*; D019843:Archaeal Proteins / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004795:Enzyme Stability; D004926:Escherichia coli / Q000235:genetics; D005778:Gefarnate / Q000031:analogs & derivatives / Q000378:metabolism; D006358:Hot Temperature; D010755:Organophosphates / Q000378:metabolism; D011994:Recombinant Proteins / Q000378:metabolism; D013379:Substrate Specificity
chemical_list: D019843:Archaeal Proteins; D010755:Organophosphates; D011994:Recombinant Proteins; D005778:Gefarnate; C043321:geranylfarnesol; D019883:Alkyl and Aryl Transferases; C023266:undecaprenyl pyrophosphate synthetase