title: α2,3-Sialyltransferase ST3Gal IV promotes migration and metastasis in pancreatic adenocarcinoma cells and tends to be highly expressed in pancreatic adenocarcinoma tissues.
abstract: Sialyltransferases have received much attention recently as they are frequently up-regulated in cancer cells. However, the role played by each sialyltransferase in tumour progression is still unknown. α2,3-Sialyltransferases ST3Gal III and ST3Gal IV are involved in sialyl-Lewis(x) (SLe(x)) synthesis. Given that the role of ST3Gal III in pancreatic adenocarcinoma cells has been previously reported, in this study we have focused on investigating the role of ST3Gal IV in the acquisition of adhesive, migratory and metastatic capabilities and, secondly, in analyzing the expression of ST3Gal III and ST3Gal IV in pancreatic adenocarcinoma tissues versus control tissues. ST3Gal IV overexpressing pancreatic adenocarcinoma MDAPanc-28 cell lines were generated. They showed a heterogeneous increase in SLe(x), and enhanced E-selectin adhesion and migration. Furthermore, when injected into nude mice, increased metastasis and decreased survival were found in comparison with controls. The behaviour of MDAPanc-28 ST3Gal IV overexpressing cells in these processes was similar to the already reported MDAPanc-28 ST3Gal III overexpressing cells. Furthermore, pancreatic adenocarcinoma tissues tended to express high levels of ST3Gal III and ST3Gal IV together with other fucosyltransferase genes FUT3 and FUT6, all involved in the last steps of sialyl-Lewis(x) biosynthesis. In conclusion, both α2,3-sialyltransferases are involved in key steps of pancreatic tumour progression processes and are highly expressed in most pancreatic adenocarcinoma tissues.
authors.affiliation: Department of Biology, University of Girona, 17071 Girona, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Pérez-Garay
authors.forename: Beatriz
authors.initials: B
authors.lastname: Arteta
authors.forename: Esther
authors.initials: E
authors.lastname: Llop
authors.forename: Lara
authors.initials: L
authors.lastname: Cobler
authors.forename: Lluís
authors.initials: L
authors.lastname: Pagès
authors.forename: Rosa
authors.initials: R
authors.lastname: Ortiz
authors.forename: María José
authors.initials: MJ
authors.lastname: Ferri
authors.forename: Carme
authors.initials: C
authors.lastname: de Bolós
authors.forename: Joan
authors.initials: J
authors.lastname: Figueras
authors.forename: Rafael
authors.initials: R
authors.lastname: de Llorens
authors.forename: Fernando
authors.initials: F
authors.lastname: Vidal-Vanaclocha
authors.forename: Rosa
authors.initials: R
authors.lastname: Peracaula
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology* / Q000235:genetics / Q000473:pathology*; D000368:Aged; D000818:Animals; D002462:Cell Membrane / Q000201:enzymology; D002465:Cell Movement / Q000235:genetics; D019040:E-Selectin / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D019158:N-Acetylneuraminic Acid / Q000378:metabolism; D009362:Neoplasm Metastasis; D009844:Oligosaccharides / Q000378:metabolism; D010190:Pancreatic Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology*; D011485:Protein Binding; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000080506:Sialyl Lewis X Antigen; D012799:Sialyltransferases / Q000235:genetics / Q000378:metabolism*; D000094663:beta-Galactoside alpha-2,3-Sialyltransferase
chemical_list: D019040:E-Selectin; D009844:Oligosaccharides; D012333:RNA, Messenger; D000080506:Sialyl Lewis X Antigen; D012799:Sialyltransferases; D019158:N-Acetylneuraminic Acid; D000094663:beta-Galactoside alpha-2,3-Sialyltransferase
keywords: Metastasis; Migration; Pancreatic adenocarcinoma; Sialyl-Lewis(x); α2,3-Sialyltransferases