title: Development of amperometric biosensors based on nanostructured tyrosinase-conducting polymer composite electrodes.
abstract: Bio-composite coatings consisting of poly(3,4-ethylenedioxythiophene) (PEDOT) and tyrosinase (Ty) were successfully electrodeposited on conventional size gold (Au) disk electrodes and microelectrode arrays using sinusoidal voltages. Electrochemical polymerization of the corresponding monomer was carried out in the presence of various Ty amounts in aqueous buffered solutions. The bio-composite coatings prepared using sinusoidal voltages and potentiostatic electrodeposition methods were compared in terms of morphology, electrochemical properties, and biocatalytic activity towards various analytes. The amperometric biosensors were tested in dopamine (DA) and catechol (CT) electroanalysis in aqueous buffered solutions. The analytical performance of the developed biosensors was investigated in terms of linear response range, detection limit, sensitivity, and repeatability. A semi-quantitative multi-analyte procedure for simultaneous determination of DA and CT was developed. The amperometric biosensor prepared using sinusoidal voltages showed much better analytical performance. The Au disk biosensor obtained by 50 mV alternating voltage amplitude displayed a linear response for DA concentrations ranging from 10 to 300 μM, with a detection limit of 4.18 μM.
authors.affiliation: Department of Analytical Chemistry and Environmental Engineering, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Bucharest 011061, Romania. stelianl@yahoo.com
authors.forename: Stelian
authors.initials: S
authors.lastname: Lupu
authors.forename: Cecilia
authors.initials: C
authors.lastname: Lete
authors.forename: Paul Cătălin
authors.initials: PC
authors.lastname: Balaure
authors.forename: Dan Ion
authors.initials: DI
authors.lastname: Caval
authors.forename: Constantin
authors.initials: C
authors.lastname: Mihailciuc
authors.forename: Boris
authors.initials: B
authors.lastname: Lakard
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Hihn
authors.forename: Francisco
authors.initials: F
authors.lastname: Javier del Campo
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry*; D002396:Catechols / Q000032:analysis; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004298:Dopamine / Q000032:analysis; D004553:Electric Conductivity; D004563:Electrochemistry / Q000379:methods*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D018625:Microscopy, Atomic Force; D014442:Monophenol Monooxygenase / Q000378:metabolism*; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D011108:Polymers / Q000737:chemistry*; D012996:Solutions; D013499:Surface Properties
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D002396:Catechols; D020099:Coated Materials, Biocompatible; D004800:Enzymes, Immobilized; D011108:Polymers; D012996:Solutions; C121383:poly(3,4-ethylene dioxythiophene); D014442:Monophenol Monooxygenase; C034221:catechol; D004298:Dopamine