title: A SOE-PCR method of introducing multiple mutations into Mycoplasma gallisepticum neuraminidase.
abstract: A modified splicing with overlap extension PCR (SOE-PCR) was generated to introduce 21 TGA to TGG at Mycoplasma gallisepticum MGA_0329 gene. The recombinant protein was successfully expressed and retained neuraminidase activities, indicating that SOE-PCR is a rapid and highly efficient method of introducing multiple mutations into large M. gallisepticum genes.
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, P. R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Tan
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, P. R. China.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Chen
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, P. R. China.
authors.forename: Shengqing
authors.initials: S
authors.lastname: Yu
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, P. R. China.
authors.forename: Xusheng
authors.initials: X
authors.lastname: Qiu
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, P. R. China.
authors.forename: Cuiping
authors.initials: C
authors.lastname: Song
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, P. R. China.
authors.forename: Danqing
authors.initials: D
authors.lastname: Chen
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, P. R. China.
authors.forename: Shilei
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, P. R. China.
authors.forename: Fanqing
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, P. R. China.
authors.forename: Suibin
authors.initials: S
authors.lastname: He
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, P. R. China; Poultry Institute, Chinese Academy of Agricultural Sciences, Yangzhou 225009, P. R. China.
authors.forename: Xinyue
authors.initials: X
authors.lastname: Shen
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, P. R. China.
authors.forename: Meirong
authors.initials: M
authors.lastname: Hu
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, P. R. China. Electronic address: shoveldeen@shvri.ac.cn.
authors.forename: Chan
authors.initials: C
authors.lastname: Ding
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D016297:Mutagenesis, Site-Directed / Q000379:methods*; D045686:Mycoplasma gallisepticum / Q000201:enzymology* / Q000235:genetics; D009439:Neuraminidase / Q000235:genetics* / Q000378:metabolism; D017354:Point Mutation; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D009439:Neuraminidase
keywords: M. gallisepticum; MGA_0329 gene; Neuraminidase; SOE-PCR