title: Expression of huCdc7 in colorectal cancer.
abstract: OBJECTIVE
  To detect the expression of huCdc7 in colorectal cancer.
  
  
  METHODS
  The mRNA and protein expression of huCdc7 in 39 colorectal cancer tissue specimens and matched tumor-adjacent normal colorectal tissue specimens was detected by reverse transcription-polymerase chain reaction and immunohistochemistry, respectively.
  
  
  RESULTS
  The relative expression level of huCdc7 mRNA in colorectal cancer was significantly higher than that in tumor-adjacent normal colorectal tissues (0.03675 ± 1.00 vs 0.01199 ± 0.44, P < 0.05). huCdc7-positive cells displayed brown granules in the nucleus. Tumor tissues contained many huCdc7-positive cells, whereas normal colorectal tissues contained very few positive cells.
  
  
  CONCLUSIONS
  huCdc7 may play an important role in the development and progression of colorectal cancer.
authors.affiliation: Department of Oncology, Kunshan Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Kunshan 215300, Jiangsu Province, China. ksschj@126.com
authors.forename: Hai-Jun
authors.initials: HJ
authors.lastname: Chen
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhu
authors.forename: Xue-Lin
authors.initials: XL
authors.lastname: Wang
authors.forename: Quan-Lin
authors.initials: QL
authors.lastname: Feng
authors.forename: Qing
authors.initials: Q
authors.lastname: Wu
authors.forename: Zheng-Ping
authors.initials: ZP
authors.lastname: Xu
authors.forename: Jiang
authors.initials: J
authors.lastname: Wu
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Yu
authors.forename: Hong-Liang
authors.initials: HL
authors.lastname: Qian
authors.forename: Qi
authors.initials: Q
authors.lastname: Lu
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics; D001706:Biopsy; D018797:Cell Cycle Proteins / Q000032:analysis* / Q000235:genetics; D015179:Colorectal Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D017346:Protein Serine-Threonine Kinases / Q000032:analysis* / Q000235:genetics; D012333:RNA, Messenger / Q000032:analysis; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; D018797:Cell Cycle Proteins; D012333:RNA, Messenger; C068619:CDC7 protein, human; D017346:Protein Serine-Threonine Kinases
keywords: Colorectal cancer; Semiquantitative reverse transcription-polymerase chain reaction; huCdc7