title: Effects of epidermal growth factor and basic fibroblast growth factor on the proliferation and osteogenic and neural differentiation of adipose-derived stem cells.
abstract: Stem cells used for clinical tissue regeneration therapy should have the capacity of self-renewal, high proliferation, and differentiation and be able to be transplanted in large numbers. Although high concentrations of epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) may induce the differentiation of stem cells, these factors have been widely used to enhance the propagation of stem cells, including adipose-derived mesenchymal stem cells (ASCs). However, the effects of low concentrations of EGF and bFGF on stem cells need to be evaluated carefully. This study illustrates that low concentrations of EGF (5 ng/mL) and bFGF (10 ng/mL) increase the proliferative ability of ASCs and induce the typical spindle-shaped cell morphology. EGF and bFGF added to medium promoted neural lineage differentiation and impaired the mesodermal differentiation ability of ASCs. This study demonstrates that even low concentrations of EGF and bFGF may limit the differentiation ability of stem cells during stem cell expansion in vitro. EGF and bFGF supplementation should be carefully considered in stem cells for clinical applications.
authors.affiliation: State Key Laboratory of Bioelectronics, School of Biological Science & Medical Engineering, Southeast University, People's Republic of China.
authors.forename: Feihu
authors.initials: F
authors.lastname: Hu
authors.forename: Xiu
authors.initials: X
authors.lastname: Wang
authors.forename: Gaofeng
authors.initials: G
authors.lastname: Liang
authors.forename: Lanxin
authors.initials: L
authors.lastname: Lv
authors.forename: Yanliang
authors.initials: Y
authors.lastname: Zhu
authors.forename: Bo
authors.initials: B
authors.lastname: Sun
authors.forename: Zhongdang
authors.initials: Z
authors.lastname: Xiao
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology*; D066298:In Vitro Techniques; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D023421:Models, Animal; D010012:Osteogenesis / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D016222:Fibroblast Growth Factor 2; D004815:Epidermal Growth Factor