title: AKT1 inhibitory DNAzymes inhibit cell proliferation and migration of thyroid cancer cells.
abstract: AKT1 is a member of the serine/threoine AGC protein kinase family involved in thyroid cancer metabolism, growth, proliferation and survival. It is overexpressed in thyroid tumors. In this study, we designed two AKT1 specific DNAzymes (DRz1 and DRz2) that target AKT1 mRNA. The results showed that DRz1 could decrease the expression of AKT1 by 58%. Furthermore, DRz1 significantly inhibited cell proliferation, induced apoptosis and inhibited invasion in SW597 cells. In addition, down-regulation of survivin expression was associated with decreased caspase-3, VEGF and MMP2 in SW597 cells after 24 h. In our study, the efficacy of DRz1 in decreasing AKT1 protein levels were better than DRz2. AKT1-DRz1 might have anti-tumorigenic activity and may provide the basis for a novel therapeutic intervention in thyroid cancer treatment.
authors.affiliation: Department of Endocrinology, The People's Hospital of Jilin Province, Changchun, China.
authors.forename: Le
authors.initials: L
authors.lastname: Yang
authors.forename: Jin-Ting
authors.initials: JT
authors.lastname: He
authors.forename: Hong
authors.initials: H
authors.lastname: Guan
authors.forename: Ya-Dong
authors.initials: YD
authors.lastname: Sun
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D002465:Cell Movement; D049109:Cell Proliferation; D021881:DNA, Catalytic / Q000494:pharmacology*; D005434:Flow Cytometry; D006801:Humans; D009361:Neoplasm Invasiveness; D051057:Proto-Oncogene Proteins c-akt / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D013964:Thyroid Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D021881:DNA, Catalytic; D012333:RNA, Messenger; C494918:AKT1 protein, human; D051057:Proto-Oncogene Proteins c-akt