title: Rapid Identification of Ginseng Cultivars (Panax ginseng Meyer) Using Novel SNP-Based Probes.
abstract: In order to develop a novel system for the discrimination of five ginseng cultivars (Panax ginseng Meyer), single nucleotide polymorphism (SNP) genotyping assays with real-time polymerase chain reaction were conducted. Nucleotide substitution in gDNA library clones of P. ginseng cv. Yunpoong was targeted for the SNP genotyping assay. From these SNP sites, a set of modified SNP specific fluorescence probes (PGP74, PGP110, and PGP130) and novel primer sets have been developed to distinguish among five ginseng cultivars. The combination of the SNP type of the five cultivars, Chungpoong, Yunpoong, Gopoong, Kumpoong, and Sunpoong, was identified as 'ATA', 'GCC', 'GTA', 'GCA', and 'ACC', respectively. This study represents the first report of the identification of ginseng cultivars by fluorescence probes. An SNP genotyping assay using fluorescence probes could prove useful for the identification of ginseng cultivars and ginseng seed management systems and guarantee the purity of ginseng seed.
authors.affiliation: National Institute of Horticultural & Herbal Science, Rural Development Administration, Eumseong 369-873, Korea.
authors.forename: Ick-Hyun
authors.initials: IH
authors.lastname: Jo
authors.forename: Kyong Hwan
authors.initials: KH
authors.lastname: Bang
authors.forename: Young-Chang
authors.initials: YC
authors.lastname: Kim
authors.forename: Jei-Wan
authors.initials: JW
authors.lastname: Lee
authors.forename: A-Yeon
authors.initials: AY
authors.lastname: Seo
authors.forename: Bong-Jae
authors.initials: BJ
authors.lastname: Seong
authors.forename: Hyun-Ho
authors.initials: HH
authors.lastname: Kim
authors.forename: Dong-Hwi
authors.initials: DH
authors.lastname: Kim
authors.forename: Seon-Woo
authors.initials: SW
authors.lastname: Cha
authors.forename: Yong-Gu
authors.initials: YG
authors.lastname: Cho
authors.forename: Hong-Sig
authors.initials: HS
authors.lastname: Kim
keywords: Fluorescence probes; Nucleotide substitution; Panax ginseng; Single nucleotide polymorphism genotyping