title: DNA fragmentation caused by an overdose of Zeocin.
abstract: DNA-damaging agent Zeocin is frequently used for vector selection in multiple cell types. We found that vector extracted from the Escherichia coli cells overgrown in the presence of Zeocin was generally contaminated with genomic DNA fragments. High dosage or prolonged cultivation should thus be avoided to minimize the contamination.
authors.affiliation: Bioprocess Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2-17-2-1 Tsukisamu-higashi, Toyohira-ku, Sapporo, Hokkaido 062-8517, Japan; Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo, 2-17-2-1 Tsukisamu-higashi, Toyohira-ku, Sapporo, Hokkaido 062-8517, Japan.
authors.forename: Miyuki
authors.initials: M
authors.lastname: Tsukuda
authors.forename: Kentaro
authors.initials: K
authors.lastname: Miyazaki
mesh_terms: D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism; D000903:Antibiotics, Antineoplastic / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001761:Bleomycin / Q000494:pharmacology*; D053938:DNA Fragmentation / Q000187:drug effects*; D024881:Drug Resistance, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D005822:Genetic Vectors / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D010957:Plasmids / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D012641:Selection, Genetic / Q000187:drug effects
chemical_list: D000903:Antibiotics, Antineoplastic; D001426:Bacterial Proteins; D001761:Bleomycin; C105427:Zeocin; C087954:bleomycin N-acetyltransferase; D000123:Acetyltransferases
keywords: Bleomycin resistant gene; DNA fragmentation; Overdose; Zeocin