title: Recombinant l-phenylalanine ammonia lyase from Rhodosporidium toruloides as a potential anticancer agent.
abstract: The recombinant producer strain expressing Rhodosporidium toruloides l-phenylalanine ammonia lyase (PAL) has been obtained, and a purification procedure of PAL has been developed. The purified enzyme, PAL, has the following biochemical and catalytic characteristics: Km for l-Phe of 0.49 mM, pH optimum at 8.5, and temperature optimum at 50°C. PAL exhibited a significant cytotoxic effect toward the following cell lines: MCF7 (IC50 = 1.97 U/mL), DU145 (IC50 = 7.3 U/mL), which are comparable with E. coli l-asparaginase type-II cytotoxicity in vitro. Administration of PAL (200-400 U/kg) to L5178y-bearing mice for five times (a total dose of 1000-2000 U/kg) was well tolerated and showed the increase of life span (ILS) = 12-16%, P < 0.05. Data obtained suggest that PAL from R. toruloides has a potential for cancer treatment.
authors.affiliation: Kemerovo Technological Institute of Food Industry, Kemerovo, Russia.
authors.forename: Olga O
authors.initials: OO
authors.lastname: Babich
authors.forename: Vadim S
authors.initials: VS
authors.lastname: Pokrovsky
authors.forename: Natalia Yu
authors.initials: NY
authors.lastname: Anisimova
authors.forename: Nikolai N
authors.initials: NN
authors.lastname: Sokolov
authors.forename: Alexander Yu
authors.initials: AY
authors.lastname: Prosekov
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D061986:MCF-7 Cells; D051379:Mice; D010650:Phenylalanine Ammonia-Lyase / Q000235:genetics* / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000235:genetics* / Q000494:pharmacology*; D013696:Temperature; D015003:Yeasts / Q000235:genetics / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D011994:Recombinant Proteins; D010650:Phenylalanine Ammonia-Lyase