title: Electrospun acetalated dextran scaffolds for temporal release of therapeutics.
abstract: Electrospun acetalated dextran (Ac-DEX) scaffolds were fabricated to encapsulate resiquimod, an immunomodulatory toll-like-receptor (TLR) agonist. Ac-DEX has been used to fabricate scaffolds for sustained and temporal delivery of therapeutics because it has tunable degradation rates that are dependent on its synthesis reaction time or the molecular weight of dextran. Additionally, as opposed to commonly electrospun polyesters that shift the local pH upon degradation, the degradation products of Ac-DEX are pH-neutral: dextran, an alcohol, and the metabolic byproduct acetone. Formulations of Ac-DEX with two different degradation rates were used in this study. The effects of electrospinning conditions on the scaffold size and morphology were examined as well as fibroblast adhesion as imaged with fluorescence microcopy and scanning electron microscopy. Macrophage (MΦ) viability further indicates that the scaffolds are cytocompatible. Also, the controlled release profiles of resiquimod from loaded scaffolds and nitric oxide (NO) production by MΦ incubated with these scaffolds show the potential for Ac-DEX scaffolds to be used to temporally and efficiently deliver therapeutics. Overall, we present a novel scaffold that can have tunable and unique drug release rates for tissue engineering, drug delivery, immunomodulation, and wound healing applications.
authors.affiliation: Division of Pharmaceutics, College of Pharmacy, The Ohio State University, Columbus, Ohio 43210, USA.
authors.forename: Hassan M
authors.initials: HM
authors.lastname: Borteh
authors.forename: Matthew D
authors.initials: MD
authors.lastname: Gallovic
authors.forename: Sadhana
authors.initials: S
authors.lastname: Sharma
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Peine
authors.forename: Simeng
authors.initials: S
authors.lastname: Miao
authors.forename: Deanna J
authors.initials: DJ
authors.lastname: Brackman
authors.forename: Katie
authors.initials: K
authors.lastname: Gregg
authors.forename: Yanyi
authors.initials: Y
authors.lastname: Xu
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Guo
authors.forename: Jianjun
authors.initials: J
authors.lastname: Guan
authors.forename: Eric M
authors.initials: EM
authors.lastname: Bachelder
authors.forename: Kristy M
authors.initials: KM
authors.lastname: Ainslie
mesh_terms: D000818:Animals; D002470:Cell Survival; D003911:Dextrans / Q000737:chemistry*; D007093:Imidazoles / Q000737:chemistry; D051379:Mice; D008855:Microscopy, Electron, Scanning; D041681:NIH 3T3 Cells; D009569:Nitric Oxide / Q000378:metabolism; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D003911:Dextrans; D007093:Imidazoles; D009569:Nitric Oxide; C402365:resiquimod