title: Morphogenetic and neuronal characterization of human neuroblastoma multicellular spheroids cultured under undifferentiated and all-trans-retinoic acid-differentiated conditions.
abstract: In this study, we aimed to compare the morphogenetic and neuronal characteristics between monolayer cells and spheroids. For this purpose, we established spheroid formation by growing SH-SY5Y cells on the hydrophobic surfaces of thermally-collapsed elastin-like polypeptide. After 4 days of culture, the relative proliferation of the cells within spheroids was approximately 92% of the values for monolayer cultures. As measured by quantitative assays for mRNA and protein expressions, the production of synaptophysin and neuronspecific enolase (NSE) as well as the contents of cell adhesion molecules (CAMs) and extracellular matrix (ECM) proteins are much higher in spheroids than in monolayer cells. Under the all-trans-retinoic acid (RA)-induced differentiation condition, spheroids extended neurites and further up-regulated the expression of synaptophysin, NSE, CAMs, and ECM proteins. Our data indicate that RA-differentiated SH-SY5Y neurospheroids are functionally matured neuronal architectures.
authors.affiliation: Laboratory of Biochemistry and Cellular Engineering, Division of NanoBio Technology, Daegu Gyeongbuk Institute of Science and Technology, Daegu 711-873, Korea.
authors.forename: Gwon-Soo
authors.initials: GS
authors.lastname: Jung
authors.forename: Kyeong-Min
authors.initials: KM
authors.lastname: Lee
authors.forename: Jin-Kyu
authors.initials: JK
authors.lastname: Park
authors.forename: Seong-Kyoon
authors.initials: SK
authors.lastname: Choi
authors.forename: Won Bae
authors.initials: WB
authors.lastname: Jeon
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D006801:Humans; D016501:Neurites / Q000187:drug effects; D009447:Neuroblastoma; D016133:Polymerase Chain Reaction; D018874:Spheroids, Cellular / Q000187:drug effects* / Q000648:ultrastructure; D014212:Tretinoin / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D014212:Tretinoin