title: Detection of bovine leukemia virus in brains of cattle with a neurological syndrome: pathological and molecular studies.
abstract: Bovine leukemia virus (BLV) was investigated in the central nervous system (CNS) of cattle with neurological syndrome. A total of 269 CNS samples were submitted to nested-PCR (BLV env gene gp51), and the viral genotypes were identified. The nested-PCR was positive in 4.8% (13/269) CNS samples, with 2.7% (2/74) presenting at histological examination lesions of nonpurulent meningoencephalitis (NPME), whereas 5.6% (11/195) not presenting NPME (P > 0.05). No samples presented lymphosarcoma. The PCR products (437 bp) were sequenced and submitted to phylogenetic analysis by neighbor-joining and maximum composite likelihood methods, and genotypes 1, 5, and 6 were detected, corroborating other South American studies. The genotype 6 barely described in Brazil and Argentina was more frequently detected in this study. The identity matrices showed maximum similarity (100%) among some samples of this study and one from Argentina (FJ808582), recovered from GenBank. There was no association among the genotypes and NPME lesions.
authors.affiliation: Pós-Graduação em Sanidade, Segurança Alimentar e Ambiental no Agronegócio, Instituto Biológico de São Paulo (IB), Avenida Conselheiro Rodrigues Alves 1252, Bairro Vila Mariana, 04014-002 São Paulo, SP, Brazil. rhrdangelino@yahoo.com.br
authors.forename: Rubens Henrique Ramos
authors.initials: RH
authors.lastname: D'Angelino
authors.forename: Edviges Maristela
authors.initials: EM
authors.lastname: Pituco
authors.forename: Eliana Monteforte Cassaro
authors.initials: EM
authors.lastname: Villalobos
authors.forename: Ricardo
authors.initials: R
authors.lastname: Harakava
authors.forename: Fábio
authors.initials: F
authors.lastname: Gregori
authors.forename: Claudia
authors.initials: C
authors.lastname: Del Fava
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001118:Argentina; D001483:Base Sequence; D001921:Brain / Q000473:pathology / Q000821:virology*; D001938:Brazil; D002417:Cattle; D005838:Genotype; D006801:Humans; D001909:Leukemia Virus, Bovine / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D009422:Nervous System Diseases / Q000235:genetics / Q000473:pathology* / Q000662:veterinary / Q000821:virology; D010802:Phylogeny; D016415:Sequence Alignment; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D014759:Viral Envelope Proteins