title: Expression, crystallization and preliminary crystallographic study of GluB from Corynebacterium glutamicum.
abstract: GluB is a substrate-binding protein (SBP) which participates in the uptake of glutamic acid in Corynebacterium glutamicum, a Gram-positive bacterium. It is part of an ATP-binding cassette (ABC) transporter system. Together with the transmembrane proteins GluC and GluD and the cytoplasmic protein GluA, which couples the hydrolysis of ATP to the translocation of glutamate, they form a highly active glutamate-uptake system. As part of efforts to study the amino-acid metabolism, especially the metabolism of glutamic acid by C. glutamicum, a bacterium that is widely used in the industrial production of glutamic acid, the GluB protein was expressed, purified and crystallized, an X-ray diffraction data set was collected to a resolution of 1.9 Å and preliminary crystallographic analysis was performed. The crystal belonged to space group P3(1)21 or P3(2)21, with unit-cell parameters a = b = 82.50, c = 72.69 Å.
authors.affiliation: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, People's Republic of China.
authors.forename: Qingbo
authors.initials: Q
authors.lastname: Liu
authors.forename: Defeng
authors.initials: D
authors.lastname: Li
authors.forename: Yonglin
authors.initials: Y
authors.lastname: Hu
authors.forename: Da Cheng
authors.initials: da C
authors.lastname: Wang
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry; D048230:Corynebacterium glutamicum; D003460:Crystallization; D018360:Crystallography, X-Ray; D015964:Gene Expression Regulation, Bacterial; D033902:Periplasmic Binding Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics
chemical_list: D018528:ATP-Binding Cassette Transporters; D001426:Bacterial Proteins; C421007:GluB protein, Corynebacterium glutamicum; D033902:Periplasmic Binding Proteins
keywords: Corynebacterium glutamicum; glutamic acid binding protein; glutamic acid metabolism