title: Broad HPV distribution in the genital region of men from the HPV infection in men (HIM) study.
abstract: The HPV infection in men (HIM) study examines the natural history of genital HPV infection in men. Genotyping methods used in this study identify 37 α-HPV types; however, the viral type could not be identified in approximately 22% of male genital specimens that were HPV PCR positive. Our aim was to genotype HPV-unclassified specimens by sequencing PGMY09/11, GP5+/6+ or FAP59/64 PCR products. Using this approach we were able to detect 86 unique HPV types among 508 of 931 specimens analyzed. We report for the first time the presence of a broad range of α-, β- and γ-HPV at the male genitals.
authors.affiliation: Molecular Biology Laboratory, Center of Translational Oncology, Instituto do Câncer do Estado de São Paulo--ICESP, São Paulo 01246-000, Brazil. lsichero@gmail.com
authors.forename: Laura
authors.initials: L
authors.lastname: Sichero
authors.forename: Christine M
authors.initials: CM
authors.lastname: Pierce Campbell
authors.forename: Silvaneide
authors.initials: S
authors.lastname: Ferreira
authors.forename: João S
authors.initials: JS
authors.lastname: Sobrinho
authors.forename: Maria
authors.initials: M
authors.lastname: Luiza Baggio
authors.forename: Lenice
authors.initials: L
authors.lastname: Galan
authors.forename: Roberto C
authors.initials: RC
authors.lastname: Silva
authors.forename: Eduardo
authors.initials: E
authors.lastname: Lazcano-Ponce
authors.forename: Anna R
authors.initials: AR
authors.lastname: Giuliano
authors.forename: Luisa L
authors.initials: LL
authors.lastname: Villa
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001938:Brazil / Q000453:epidemiology; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D005431:Florida / Q000453:epidemiology; D005837:Genitalia, Male / Q000821:virology*; D005838:Genotype; D006801:Humans; D008297:Male; D008800:Mexico / Q000453:epidemiology; D008875:Middle Aged; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000821:virology; D016133:Polymerase Chain Reaction / Q000379:methods; D055815:Young Adult
chemical_list: D004279:DNA, Viral
keywords: Genera; Genital; Human papillomavirus; Men; Penile; Viral prevalence