title: ELISA detection of semicarbazide based on a fast sample pretreatment method.
abstract: A direct ELISA was established for the fast detection of semicarbazide (SEM) using a novel biotin derivative. Without a tedious extraction procedure, as low as 0.07 μg L(-1) of SEM could be detected reproducibly. This assay has better recovery and accuracy than competitive ELISA.
authors.affiliation: Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Fang
authors.forename: Baishan
authors.initials: B
authors.lastname: Jiang
authors.forename: Wei
authors.initials: W
authors.lastname: Wu
authors.forename: Zhicheng
authors.initials: Z
authors.lastname: Xiang
authors.forename: Chuanyan
authors.initials: C
authors.lastname: Ouyang
authors.forename: Tulong
authors.initials: T
authors.lastname: Huang
authors.forename: Junhua
authors.initials: J
authors.lastname: Chen
authors.forename: Lingwen
authors.initials: L
authors.lastname: Zeng
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D001710:Biotin / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005506:Food Contamination / Q000032:analysis*; D008892:Milk / Q000737:chemistry; D012663:Semicarbazides / Q000032:analysis* / Q000276:immunology
chemical_list: D000906:Antibodies; D012663:Semicarbazides; C010059:carbamylhydrazine; D001710:Biotin