title: In vitro biocompatibility of Ti-45S5 bioglass nanocomposites and their scaffolds.
abstract: Titanium-10 wt % 45S5 Bioglass nanocomposites and their scaffolds were prepared by mechanical alloying (MA) followed by pressing, sintering, or combination of MA and a "space-holder" sintering process, respectively. An amorphous structure was obtained at 15 h of milling. The crystallization of the amorphous phase upon annealing led to the formation of a nanostructured Ti-10 wt % 45S5 Bioglass composite with a grain size of approximately 7 nm. The in vitro cytocompatibility of these materials was evaluated and compared with a conventional microcrystalline titanium. During the studies, established cell line of human fibroblasts CCD-39Lu was cultured in the presence of tested materials and its survival rate, and proliferation activity were examined. Furthermore, the influence of the Ti-45S5 Bioglass nanocomposites and microcrystalline titanium was tested on the growth of Candida albicans yeast. Biocompatibility tests carried out indicate that the nanocomposite Ti-10 wt % 45S5 Bioglass scaffolds could be a possible candidate for dental implants and other medicinal applications.
authors.affiliation: Department of Immunology, Poznan University of Medical Sciences, Rokietnicka 5D, 60-806, Poznan, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Kaczmarek
authors.forename: M U
authors.initials: MU
authors.lastname: Jurczyk
authors.forename: B
authors.initials: B
authors.lastname: Rubis
authors.forename: A
authors.initials: A
authors.lastname: Banaszak
authors.forename: A
authors.initials: A
authors.lastname: Kolecka
authors.forename: A
authors.initials: A
authors.lastname: Paszel
authors.forename: K
authors.initials: K
authors.lastname: Jurczyk
authors.forename: M
authors.initials: M
authors.lastname: Murias
authors.forename: J
authors.initials: J
authors.lastname: Sikora
authors.forename: M
authors.initials: M
authors.lastname: Jurczyk
mesh_terms: D001672:Biocompatible Materials / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects / Q000254:growth & development; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002516:Ceramics / Q000494:pharmacology*; D015169:Colony Count, Microbial; D005898:Glass; D006801:Humans; D008422:Materials Testing; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D011419:Propidium / Q000378:metabolism; D054457:Tissue Scaffolds / Q000737:chemistry*; D014025:Titanium / Q000494:pharmacology*
chemical_list: D001672:Biocompatible Materials; C425330:bioactive glass 45S5; D011419:Propidium; D014025:Titanium
keywords: bioglass; cell proliferation; cell viability; nanocomposite; scaffold