title: Dietary fish oil normalized glucose-stimulated insulin secretion in isolated pancreatic islets of dyslipemic rats through mechanisms involving glucose phosphorylation, peroxisome proliferator-activated receptor γ and uncoupling protein 2.
abstract: This study evaluates some possible mechanisms behind the beneficial effects of dietary fish oil (FO) on β cell dysfunction in rats fed a sucrose-rich diet (SRD). Rats were fed a SRD for 6 months. Thereafter, half the rats received a SRD in which corn oil was partially replaced by FO up to 8 months. The other half continued consuming the SRD up to 8 months. A control group was fed a control diet throughout the experimental period. In isolated islets of SRD-fed rats dietary FO normalized the reduced glucose phosphorylation, the altered glucose oxidation, the triglyceride content, the increased protein mass levels of peroxisome proliferator-activated receptor γ (PPARγ) and uncoupling protein 2 without changes in GLUT2 and PPARα. These finding suggest that the changes mentioned above could be involved in the normalization of the altered glucose-stimulated insulin secretion pattern in this nutritional model of dyslipidemia and insulin resistance.
authors.affiliation: Department of Biochemistry, School of Biochemistry, University of Litoral. Ciudad Universitaria Paraje El Pozo CC 242 (3000) Santa Fe, Argentina.
authors.forename: M R
authors.initials: MR
authors.lastname: Ferreira
authors.forename: A
authors.initials: A
authors.lastname: Chicco
authors.forename: Y B
authors.initials: YB
authors.lastname: Lombardo
mesh_terms: D000818:Animals; D050171:Dyslipidemias / Q000378:metabolism*; D005395:Fish Oils / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism*; D051273:Glucose Transporter Type 2 / Q000378:metabolism*; D007328:Insulin / Q000378:metabolism*; D007473:Ion Channels / Q000378:metabolism*; D007515:Islets of Langerhans / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D024101:Mitochondrial Proteins / Q000378:metabolism*; D047495:PPAR gamma / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D000071246:Uncoupling Protein 2
chemical_list: D005395:Fish Oils; D051273:Glucose Transporter Type 2; D007328:Insulin; D007473:Ion Channels; D024101:Mitochondrial Proteins; D047495:PPAR gamma; C000604391:Ucp2 protein, rat; D000071246:Uncoupling Protein 2; D005947:Glucose