title: Crystallization and preliminary X-ray diffraction analysis of tetrathionate hydrolase from Acidithiobacillus ferrooxidans.
abstract: Tetrathionate hydrolase (4THase) from the iron- and sulfur-oxidizing bacterium Acidithiobacillus ferrooxidans catalyses the disproportionate hydrolysis of tetrathionate to elemental sulfur, thiosulfate and sulfate. The gene encoding 4THase (Af-tth) was expressed as inclusion bodies in recombinant Escherichia coli. Recombinant Af-Tth was activated by refolding under acidic conditions and was then purified to homogeneity. The recombinant protein was crystallized in 20 mM glycine buffer pH 10 containing 50 mM sodium chloride and 33%(v/v) PEG 1000 using the hanging-drop vapour-diffusion method. The crystal was a hexagonal cylinder with dimensions of 0.2 × 0.05 × 0.05 mm. X-ray crystallographic analysis showed that the crystal diffracted to 2.15 Å resolution and belongs to space group P3(1) or P3(2), with unit-cell parameters a = b = 92.1, c = 232.6 Å.
authors.affiliation: Department of Biofunctional Chemistry, Division of Agricultural and Life Science, Graduate School of Environmental and Life Science, Okayama University, 3-1-1 Tsushima-Naka, Kita-ku, Okayama 700-8530, Japan.
authors.forename: Tadayoshi
authors.initials: T
authors.lastname: Kanao
authors.forename: Megumi
authors.initials: M
authors.lastname: Kosaka
authors.forename: Kyoya
authors.initials: K
authors.lastname: Yoshida
authors.forename: Hisayuki
authors.initials: H
authors.lastname: Nakayama
authors.forename: Taro
authors.initials: T
authors.lastname: Tamada
authors.forename: Ryota
authors.initials: R
authors.lastname: Kuroki
authors.forename: Hidenori
authors.initials: H
authors.lastname: Yamada
authors.forename: Jun
authors.initials: J
authors.lastname: Takada
authors.forename: Kazuo
authors.initials: K
authors.lastname: Kamimura
mesh_terms: D042763:Acidithiobacillus / Q000201:enzymology*; D001426:Bacterial Proteins / Q000032:analysis / Q000737:chemistry*; D003460:Crystallization; D006867:Hydrolases / Q000032:analysis / Q000737:chemistry*; D011994:Recombinant Proteins / Q000032:analysis / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D006867:Hydrolases; C104756:tetrathionate hydrolase
keywords: Acidithiobacillus ferrooxidans; tetrathionate hydrolase