title: Revision of the human hematopoietic tree: granulocyte subtypes derive from distinct hematopoietic lineages.
abstract: The classical model of hematopoiesis predicts a dichotomous lineage restriction of multipotent hematopoietic progenitors (MPPs) into common lymphoid progenitors (CLPs) and common myeloid progenitors (CMPs). However, this idea has been challenged by the identification of lymphoid progenitors retaining partial myeloid potential (e.g., LMPPs), implying that granulocytes can arise within both the classical lymphoid and the myeloid branches. Here, we resolve this issue by using cell-surface CD133 expression to discriminate functional progenitor populations. We show that eosinophilic and basophilic granulocytes as well as erythrocytes and megakaryocytes derive from a common erythro-myeloid progenitor (EMP), whereas neutrophilic granulocytes arise independently within a lympho-myeloid branch with long-term progenitor function. These findings challenge the concept of a CMP and restore dichotomy to the classical hematopoietic model.
authors.affiliation: Institute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Virchowstr. 179, 45147 Essen, Germany.
authors.forename: André
authors.initials: A
authors.lastname: Görgens
authors.forename: Stefan
authors.initials: S
authors.lastname: Radtke
authors.forename: Michael
authors.initials: M
authors.lastname: Möllmann
authors.forename: Michael
authors.initials: M
authors.lastname: Cross
authors.forename: Jan
authors.initials: J
authors.lastname: Dürig
authors.forename: Peter A
authors.initials: PA
authors.lastname: Horn
authors.forename: Bernd
authors.initials: B
authors.lastname: Giebel
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D018952:Antigens, CD34 / Q000378:metabolism; D001491:Basophils / Q000166:cytology / Q000378:metabolism; D019070:Cell Lineage; D002478:Cells, Cultured; D004804:Eosinophils / Q000166:cytology / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism; D006098:Granulocytes / Q000166:cytology* / Q000378:metabolism; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D054503:Lymphoid Progenitor Cells / Q000166:cytology / Q000378:metabolism; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D009504:Neutrophils / Q000166:cytology / Q000378:metabolism; D010455:Peptides / Q000378:metabolism
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D018952:Antigens, CD34; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; C000606024:Prom1 protein, mouse