title: Aqueous-ozone irrigation of bone monocortical wounds in hyperglycemic rats.
abstract: OBJECTIVE
  To analyze the effects of aqueous ozone irrigation over bone healing in hyperglycemia-induced rats.
  
  
  METHODS
  Forty-eight male Wistar rats were allocated into Group H (hyperglycemic) or Group N (control). Monocortical bone wound were performed on femurs' anterolateral face. Wounds were treated with a trans-operatory single irrigation of 100ml of aqueous ozone [0.004mg/ml] whereas control groups received 100ml of pure water (Milli-Q®). Histomorphological and histomorphometrical analyses were accomplished after seven, 14 and 21 days. Kruskal-Wallis and Mann-Whitney statistical tests were applied for bone neoformation quantification and assessment.
  
  
  RESULTS
  Aqueous ozone wounds irrigated revealed diffuse hemorrhage and increased neoformed of blood vessels number. There was no statistical significant difference in bone trabeculae neoformation. After seven and 14 days, the number of osteoclasts was higher in aqueous ozone groups than in those treated with pure water.
  
  
  CONCLUSIONS
  Independently of blood glucose levels, aqueous ozone allowed an increase in blood vessels neoformation and osteoclast migration, without affect bone trabeculae neoformation.
authors.affiliation: Oral Pathology Department, Dentistry School, University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Alexandre Viana
authors.initials: AV
authors.lastname: Frascino
authors.forename: Andrea
authors.initials: A
authors.lastname: Mantesso
authors.forename: Luciana
authors.initials: L
authors.lastname: Corrêa
authors.forename: Maria Cristina Zindel
authors.initials: MC
authors.lastname: Deboni
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000187:drug effects; D001861:Bone Regeneration / Q000187:drug effects*; D006943:Hyperglycemia / Q000503:physiopathology*; D008297:Male; D010010:Osteoclasts / Q000166:cytology / Q000187:drug effects; D010126:Ozone / Q000627:therapeutic use*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012016:Reference Values; D007507:Therapeutic Irrigation / Q000379:methods; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
chemical_list: D001786:Blood Glucose; D010126:Ozone