title: Nanobody-functionalized polymersomes for tumor-vessel targeting.
abstract: Targeted carrier systems (e.g., liposomes or nanoparticles) are used to specifically deliver drugs to a site of interest. Site-direction can be achieved by attachment of targeting molecules, such as peptides, DNA/RNA, or antibodies, to the surface of the carrier. Here, the formation of polymersomes with tumor-targeting potential is described. A single-domain antibody (A12) that specifically targets PlexinD1 (a transmembrane protein overexpressed in tumor vasculature) is equipped with an azide-functionality using expressed protein ligation. This azide-containing A12 can subsequently be attached to BCN-functionalized polymersomes using a strain-promoted azide alkyne cycloaddition, thereby forming polymersomes with tumor-targeting potential.
authors.affiliation: Radboud University Nijmegen, Institute for Molecules and Materials, Nijmegen The Netherlands.
authors.forename: Marjoke F
authors.initials: MF
authors.lastname: Debets
authors.forename: William P J
authors.initials: WP
authors.lastname: Leenders
authors.forename: Kiek
authors.initials: K
authors.lastname: Verrijp
authors.forename: Marleen
authors.initials: M
authors.lastname: Zonjee
authors.forename: Silvie A
authors.initials: SA
authors.lastname: Meeuwissen
authors.forename: Irene
authors.initials: I
authors.lastname: Otte-Höller
authors.forename: Jan C M
authors.initials: JC
authors.lastname: van Hest
mesh_terms: D000818:Animals; D001386:Azides / Q000737:chemistry; D015816:Cell Adhesion Molecules, Neuronal / Q000737:chemistry / Q000276:immunology; D016503:Drug Delivery Systems; D006046:Gold / Q000008:administration & dosage / Q000737:chemistry; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008081:Liposomes / Q000008:administration & dosage / Q000737:chemistry*; D008562:Membrane Glycoproteins; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D051379:Mice; D058990:Molecular Targeted Therapy; D009369:Neoplasms / Q000188:drug therapy* / Q000276:immunology; D061905:Single-Domain Antibodies / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D001386:Azides; D015816:Cell Adhesion Molecules, Neuronal; D047908:Intracellular Signaling Peptides and Proteins; D008081:Liposomes; D008562:Membrane Glycoproteins; C493082:PLXND1 protein, human; D061905:Single-Domain Antibodies; D006046:Gold
keywords: bioconjugation; expressed protein ligation; polymersome decoration; strain-promoted azide alkyne cycloaddition; tumor-targeting polymersomes