title: Molecular epidemiology, plasmid analysis, virulence, and resistance of Escherichia coli isolated from neonatal intensive care units in Poland.
abstract: We investigated the set of Escherichia coli isolates originating from newborns in relation to resistance, virulence factors (VFs), phylogenetic groups, plasmid replicon typing, and genotypes. The most isolates were clustered in ECOR group B2. Extended-spectrum beta-lactamase phenotype was found in 27.7% of isolates. The ST131 clone was detected among 33 strains, 12 of which carried the CTX-M-15 gene. Most VFs were detected among ST131 isolates and in the B2 group. IbeA gene was found more frequently in the blood isolates, while the iha gene, in the urine isolates. The 3 most prevalent replicon types were IncFIB, IncF, and IncFIA.
authors.affiliation: Jagiellonian University Medical College, 31-121 Krakow, Poland. achmiel@cm-uj.krakow.pl
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Chmielarczyk
authors.forename: Monika
authors.initials: M
authors.lastname: Pobiega
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Wójkowska-Mach
authors.forename: Dorota
authors.initials: D
authors.lastname: Romaniszyn
authors.forename: Paweł
authors.initials: P
authors.lastname: Adamski
authors.forename: Piotr B
authors.initials: PB
authors.lastname: Heczko
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Bulanda
mesh_terms: D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D015373:Bacterial Typing Techniques; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000453:epidemiology* / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007231:Infant, Newborn; D007363:Intensive Care Units, Neonatal; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D010802:Phylogeny; D010957:Plasmids; D011044:Poland / Q000453:epidemiology; D012093:Replicon; D037521:Virulence Factors / Q000235:genetics* / Q000378:metabolism; D001618:beta-Lactamases / Q000145:classification / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; C096350:CusC protein, E coli; D029968:Escherichia coli Proteins; D008565:Membrane Proteins; D037521:Virulence Factors; D001618:beta-Lactamases
keywords: E. coli; Genotyping; Plasmid replicon typing; Resistance; Virulence