title: Improved radiometabolite analysis procedure for positron emission tomography (PET) radioligands using a monolithic column coupled with direct injection micellar/high submicellar liquid chromatography.
abstract: A high-speed and high-sensitive direct plasma radiometabolite analysis method for positron emission tomography (PET) radioligands has been developed. It was based on micellar/high submicellar liquid chromatography (LC) using a semi-preparative alkyl-bonded silica rod column in conjunction with steep gradient and high flow rate for rapid macromolecule removal from plasma without significant sample preparation and fast/efficient separation of PET radioligands from their radiometabolites. Excellent separation of target PET radioligand from its radiometabolites was achieved within 4 min with a limit of detection of 1 Bq (Bq) level. This method was successfully applied to study the radiometabolism for a wide variety of (11)C and (18)F labeled radioligands in human or monkey plasma. The improved sensitivity and throughput permitted the analysis of a large number of plasma samples for accurate determination PET radioligands during quantitative PET imaging studies. Micellar/high submicellar LC together with a monolithic column is an attractive alternative method to determine the radiometabolism of PET radioligands in plasma.
authors.affiliation: Karolinska Institutet, Department of Clinical Neuroscience, Center for Psychiatric Research, Stockholm, Sweden. ryuji.nakao@ki.se
authors.forename: Ryuji
authors.initials: R
authors.lastname: Nakao
authors.forename: Christer
authors.initials: C
authors.lastname: Halldin
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D000882:Haplorhini; D006801:Humans; D008823:Micelles; D049268:Positron-Emission Tomography / Q000379:methods; D019275:Radiopharmaceuticals / Q000097:blood* / Q000493:pharmacokinetics; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D008823:Micelles; D019275:Radiopharmaceuticals; D012822:Silicon Dioxide