title: Colorimetric detection of ractopamine and salbutamol using gold nanoparticles functionalized with melamine as a probe.
abstract: A highly selective and sensitive method is developed for colorimetric detection of ractopamine and salbutamol using gold nanoparticles (AuNPs) functionalized with melamine (MA), respectively. The presence of these β-agonists induces the aggregation of gold nanoparticles through hydrogen-bonding interaction that was accompanied by a distinct change in color and optical properties, which could be monitored by a UV-vis spectrophotometer or even naked eyes. This process caused a significant decrease in the absorbance ratio (A670 nm/A520 nm) of melamine-gold nanoparticles (MA-AuNPs), and the color changed from wine red to blue. The systems exhibited a wide liner range, from 1×10(-10)M to 5×10(-7)mol/L with a correlation coefficient of 0.995 for ractopamine, and 1×10(-10)M to 1×10(-5)mol/L with a correlation coefficient of 0.996 for salbutamol, with measuring the absorbance ratio (A670 nm/A520 nm). The detection limit of these β-agonists is as low as 1×10(-11)mol/L. Particularly, the developed method has been applied to the analysis of real swine feed samples and has achieved satisfactory results.
authors.affiliation: School of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, 19A YuQuan Road, Beijing 100049, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.forename: Peilong
authors.initials: P
authors.lastname: Wang
authors.forename: Xiaoou
authors.initials: X
authors.lastname: Su
authors.forename: Hong
authors.initials: H
authors.lastname: Zhao
authors.forename: Yujian
authors.initials: Y
authors.lastname: He
mesh_terms: D058666:Adrenergic beta-2 Receptor Agonists / Q000032:analysis*; D000420:Albuterol / Q000032:analysis*; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D003124:Colorimetry / Q000379:methods; D005506:Food Contamination / Q000032:analysis; D006046:Gold / Q000737:chemistry; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D010627:Phenethylamines / Q000032:analysis*; D013552:Swine; D014227:Triazines / Q000737:chemistry
chemical_list: D058666:Adrenergic beta-2 Receptor Agonists; D010627:Phenethylamines; D014227:Triazines; C060897:ractopamine; D006046:Gold; C011907:melamine; D000420:Albuterol