title: Comparative study of microRNA profiling in keloid fibroblast and annotation of differential expressed microRNAs.
abstract: Keloids are tumor-like skin scars that grow as a result of the aberrant healing of skin injuries, with no effective treatment. The molecular mechanism underlying keloid pathogenesis is still largely unknown. In this study, we compared microRNA (miRNA) expression profiles between keloid-derived fibroblasts and normal fibroblasts (including fetal and adult dermal fibroblasts) by miRNA microarray analysis. We found that the miRNA profiles in keloid-derived fibroblasts are different with those in normal fibroblasts. Nine miRNAs were differentially expressed, six of which were significantly up-regulated in keloid fibroblasts (KFs), including miR-152, miR-23b-3p, miR-31-5p, miR-320c, miR-30a-5p, and hsv1-miR-H7, and three of which were significantly down-regulated, including miR-4328, miR-145-5p, and miR-143-3p. Functional annotations of differentially expressed miRNA targets revealed that they were enriched in several signaling pathways important for scar wound healing. In conclusion, we demonstrate that the miRNA expression profile is altered in KFs compared with in fetal and adult dermal fibroblasts, and the expression profile may provide a useful clue for exploring the pathogenesis of keloids. miRNAs might partially contribute to the etiology of keloids by affecting several signaling pathways relevant to scar wound healing.
authors.affiliation: Department of Rehabilitation Medicine, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200233, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.forename: Yuehong
authors.initials: Y
authors.lastname: Bai
authors.forename: Hua
authors.initials: H
authors.lastname: Liu
authors.forename: Xuemei
authors.initials: X
authors.lastname: Zuo
authors.forename: Haichang
authors.initials: H
authors.lastname: Yao
authors.forename: Yiming
authors.initials: Y
authors.lastname: Xu
authors.forename: Manlin
authors.initials: M
authors.lastname: Cao
mesh_terms: D002478:Cells, Cultured; D016000:Cluster Analysis; D005347:Fibroblasts / Q000473:pathology; D020869:Gene Expression Profiling; D006801:Humans; D007627:Keloid / Q000378:metabolism / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D035683:MicroRNAs
keywords: adult dermis; fetal dermis; fibroblasts; keloid; microrna