title: Expression, crystallization and preliminary X-ray crystallographic analysis of aldehyde dehydrogenase (ALDH) from Bacillus cereus.
abstract: Aldehyde dehydrogenase (ALDH) catalyses the oxidation of aldehydes using NAD(P)(+) as a cofactor. Most aldehydes are toxic at low levels. ALDHs are used to regulate metabolic intermediate aldehydes. The aldh gene from Bacillus cereus was cloned and the ALDH protein was expressed, purified and crystallized. A crystal of the ALDH protein diffracted to 2.6 Å resolution and belonged to the monoclinic space group P21, with unit-cell parameters a = 83.5, b = 93.3, c = 145.5 Å, β = 98.05°. Four protomers were present in the asymmetric unit, with a corresponding VM of 2.55 Å(3) Da(-1) and a solvent content of 51.8%.
authors.affiliation: Department of Advanced Technology Fusion, Konkuk University, Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Ho Phuong Thuy
authors.initials: HP
authors.lastname: Ngo
authors.forename: Seung Hye
authors.initials: SH
authors.lastname: Hong
authors.forename: Deok Kun
authors.initials: DK
authors.lastname: Oh
authors.forename: Lin Woo
authors.initials: LW
authors.lastname: Kang
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D001409:Bacillus cereus / Q000201:enzymology*; D003460:Crystallization; D018360:Crystallography, X-Ray; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic
chemical_list: D000444:Aldehyde Dehydrogenase
keywords: ALDH; Bacillus cereus; aldehyde dehydrogenases