title: High-density, homogeneous endospore monolayer deposition on test surfaces.
abstract: Bacillus subtilis spores were deposited in high-density single layers on metal, glass, and polymer substrates using vacuum filtration followed by a wetted filter transfer step. Quantitative analysis of spore transfer was performed using culture-based and germinability assays, and spore distributions were observed with electron microscopy.
authors.affiliation: Jet Propulsion Laboratory, California Institute of Technology, 4800 Oak Grove Drive, Pasadena, CA 91109, USA.
authors.forename: Aaron C
authors.initials: AC
authors.lastname: Noell
authors.forename: Arin R
authors.initials: AR
authors.lastname: Greenwood
authors.forename: Christine M
authors.initials: CM
authors.lastname: Lee
authors.forename: Adrian
authors.initials: A
authors.lastname: Ponce
mesh_terms: D001412:Bacillus subtilis / Q000166:cytology* / Q000502:physiology; D001431:Bacteriological Techniques / Q000295:instrumentation / Q000379:methods; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods; D005374:Filtration; D008854:Microscopy, Electron; D013171:Spores, Bacterial / Q000166:cytology* / Q000502:physiology; D014618:Vacuum
keywords: B. subtilis; Deposition; Inactivation; Spores; Vacuum filtration