title: Designing a gelatin/chitosan/hyaluronic acid biopolymer using a thermophysical approach for use in tissue engineering.
abstract: Cell culture on biopolymeric scaffolds has provided treatments for tissue engineering. Biopolymeric mixtures based on gelatin (Ge), chitosan (Ch) and hyaluronic acid (Ha) have been used to make scaffolds for wound healing. Thermal and physical properties of scaffolds prepared with Ge, Ch and Ha were characterized. Thermal characterization was made by using differential scanning calorimetry (DSC), and physical characterization by gas pycnometry and scanning electron microscopy. The effects of Ge content and cross-linking on thermophysical properties were evaluated by means of a factorial experiment design (central composite face centered). Gelatin content was the main factor that affects the thermophysical properties (microstructure and thermal transitions) of the scaffold. The effect of Ge content of the scaffolds for tissue engineering was studied by seeding skin cells on the biopolymers. The cell attachment was not significantly modified at different Ge contents; however, the cell growth rate increased linearly with the decrease of the Ge content. This relationship together with the thermophysical characterization may be used to design scaffolds for tissue engineering.
authors.affiliation: Faculty of Medicine, School of Nutrition and Dietetics, School of Service Management, Universidad de los Andes, San Carlos de Apoquindo 2200, Las Condes, Santiago, Chile.
authors.forename: Javier
authors.initials: J
authors.lastname: Enrione
authors.forename: Paulo
authors.initials: P
authors.lastname: Díaz-Calderón
authors.forename: Caroline R
authors.initials: CR
authors.lastname: Weinstein-Oppenheimer
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Sánchez
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Fuentes
authors.forename: Donald I
authors.initials: DI
authors.lastname: Brown
authors.forename: Hugo
authors.initials: H
authors.lastname: Herrera
authors.forename: Cristian A
authors.initials: CA
authors.lastname: Acevedo
mesh_terms: D000818:Animals; D001704:Biopolymers / Q000737:chemistry*; D002152:Calorimetry, Differential Scanning; D002448:Cell Adhesion; D002455:Cell Division; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry*; D006820:Hyaluronic Acid / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D051381:Rats; D013696:Temperature; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D001704:Biopolymers; D005780:Gelatin; D006820:Hyaluronic Acid; D048271:Chitosan