title: Co-culture of adipose-derived stem cells and endothelial cells in fibrin induces angiogenesis and vasculogenesis in a chorioallantoic membrane model.
abstract: Neovascularization of adipose tissue equivalents is a crucial step in successful adipose tissue engineering, since insufficient vascularization results in graft resorption in an in vivo situation. A possible cellular approach to overcome this limitation is the co-implantation of adipose-derived stem cells (ASCs) with endothelial cells to stimulate the formation of a vascular network. We investigated the potential of ASCs derived from human abdominal fat tissue co-cultured with endothelial progenitor cells (EPCs) from human peripheral blood to stimulate neovascularization of fibrin constructs on the chorioallantoic membrane (CAM) of fertilized chicken eggs, in direct comparison to human umbilical vein endothelial cells (HUVECs). After 9 days of incubation, cell-fibrin constructs were explanted and histologically evaluated with respect to ingrowth of avian blood vessels into the construct and formation of human blood vessels by co-implanted endothelial cells. When administered on the CAM, ASCs successfully guided host vasculature into the construct (angiogenesis) and guided formation of capillary-like structures by co-implanted human endothelial cells (vasculogenesis), with HUVECs being superior to EPCs, leading to a perfused avian and human capillary network within the fibrin construct. However, the results also showed that perfused human blood vessels were only observed near the CAM compared to unperfused capillary-like structures near the top of the construct, indicating that perfusion of the cell-fibrin construct takes longer than 9 days. In conclusion, as blood vessel formation is an essential step during adipogenic differentiation, the data support our hypothesis that cellular communication between transplanted ASCs and endothelial cells is beneficial for vasculogenesis. Copyright © 2013 John Wiley & Sons, Ltd.
authors.affiliation: Department of Plastic and Hand Surgery, University of Freiburg Medical Centre, Hugstetter Strasse 55, 79106, Freiburg, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Strassburg
authors.affiliation: Department of Plastic and Hand Surgery, University of Freiburg Medical Centre, Hugstetter Strasse 55, 79106, Freiburg, Germany.
authors.forename: Henrik
authors.initials: H
authors.lastname: Nienhueser
authors.affiliation: Department of Plastic and Hand Surgery, University of Freiburg Medical Centre, Hugstetter Strasse 55, 79106, Freiburg, Germany.
authors.forename: G
authors.initials: G
authors.lastname: Björn Stark
authors.affiliation: Department of Plastic and Hand Surgery, University of Freiburg Medical Centre, Hugstetter Strasse 55, 79106, Freiburg, Germany.
authors.forename: Günter
authors.initials: G
authors.lastname: Finkenzeller
authors.affiliation: Department of Plastic and Hand Surgery, University of Freiburg Medical Centre, Hugstetter Strasse 55, 79106, Freiburg, Germany.
authors.forename: Nestor
authors.initials: N
authors.lastname: Torio-Padron
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000378:metabolism*; D000328:Adult; D000818:Animals; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000737:chemistry*; D018920:Coculture Techniques; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005337:Fibrin / Q000494:pharmacology*; D006801:Humans; D008297:Male; D008954:Models, Biological; D018919:Neovascularization, Physiologic; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: D005337:Fibrin
keywords: adipose-derived stem cells; angiogenesis; chorioallantoic membrane; co-culture; endothelial progenitor cells; vasculogenesis