title: Expression, purification and crystallization of the C-terminal LRR domain of Streptococcus pyogenes protein 0843.
abstract: Streptococcus pyogenes protein 0843 (Spy0843) is a recently identified protein with a potential adhesin function. Sequence analysis has shown that Spy0843 contains two leucine-rich repeat (LRR) domains that mediate interactions with the gp340 receptor. Here, the C-terminal LRR domain was overexpressed in Escherichia coli, purified and crystallized in the presence of 1.7-1.8 M ammonium sulfate pH 7.4 as precipitant. Data were collected from a single crystal to 1.59 Å resolution at 100 K at a synchrotron-radiation source. The crystal was found to belong to space group I41, with unit-cell parameters a = b = 121.4, c = 51.5 Å and one molecule in the asymmetric unit. Elucidation of the crystal structure will provide insights into the interactions of Spy0843 with the gp340 receptor and a better understanding of the role of Spy0843 in streptococcal infections.
authors.affiliation: Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, 20521 Turku, Finland.
authors.forename: Teemu
authors.initials: T
authors.lastname: Haikarainen
authors.forename: Vuokko
authors.initials: V
authors.lastname: Loimaranta
authors.forename: Carlos
authors.initials: C
authors.lastname: Prieto-Lopez
authors.forename: Pradeep
authors.initials: P
authors.lastname: Battula
authors.forename: Jukka
authors.initials: J
authors.lastname: Finne
authors.forename: Anastassios C
authors.initials: AC
authors.lastname: Papageorgiou
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification*; D003460:Crystallization; D015964:Gene Expression Regulation, Bacterial; D007930:Leucine / Q000737:chemistry / Q000378:metabolism; D017434:Protein Structure, Tertiary; D013290:Streptococcal Infections / Q000378:metabolism; D013297:Streptococcus pyogenes / Q000737:chemistry / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D007930:Leucine
keywords: Spy0843; Streptococcus pyogenes; bacterial adhesion; leucine-rich repeats; scavenger receptors