title: Homologous HAP5 subunit from Picea wilsonii improved tolerance to salt and decreased sensitivity to ABA in transformed Arabidopsis.
abstract: HAP is a ubiquitous transcription factor family which consists of three distinct subunits, namely HAP2, HAP3, and HAP5. Among them, HAP2 and HAP3 subunits have been reported to be involved in plant response to abiotic stress. Here, a HAP5 subunit was identified from Picea wilsonii Mast. and transformed to Arabidopsis to investigate its functions in plant stress response. We found that transformed Arabidopsis with over-expressing PwHAP5 exhibited higher seed germination under salinity, osmotic and abscisic acid (ABA) stress treatment compared to Col-0 plants. The seedlings of transformed Arabidopsis also showed improved tolerance to salinity and decreased sensitivity to ABA treatment. Over-expression of PwHAP5 in Arabidopsis athap5 mutant rescued partly tolerance to NaCl, mannitol and ABA treatment. Furthermore, we examined transcription levels of several stress-related genes in transformed seedlings. Among them, mRNA expression levels of COR15a, KIN1, DREB2A, and RD29A genes were substantially higher in transformed Arabidopsis than those in wild-type (Col-0) plants. Therefore, our data revealed that PwHAP5 plays positive roles in response to salinity, osmotic and ABA stress at different developmental stages in plants, respectively, via possibly regulating stress-related genes.
authors.affiliation: Key Laboratory of Forest Silviculture and Conservation of the Ministry of Education, Beijing Forestry University, Beijing, People's Republic of China.
authors.forename: Lingli
authors.initials: L
authors.lastname: Li
authors.forename: Yanli
authors.initials: Y
authors.lastname: Yu
authors.forename: Jing
authors.initials: J
authors.lastname: Wei
authors.forename: Guixue
authors.initials: G
authors.lastname: Huang
authors.forename: Dun
authors.initials: D
authors.lastname: Zhang
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Lingyun
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D001483:Base Sequence; D018506:Gene Expression Regulation, Plant; D018525:Germination; D008353:Mannitol / Q000494:pharmacology; D008969:Molecular Sequence Data; D009154:Mutation; D009997:Osmotic Pressure; D010641:Phenotype; D028222:Picea / Q000235:genetics*; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D012639:Seeds / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000494:pharmacology; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
chemical_list: D010937:Plant Growth Regulators; D010940:Plant Proteins; D014157:Transcription Factors; D008353:Mannitol; D012965:Sodium Chloride; D000040:Abscisic Acid