title: Real-time monitoring the spatiotemporal dynamics of intracellular cGMP in vascular smooth muscle cells.
abstract: Real-time and noninvasive imaging of intracellular second messengers in mammalian cells, while -preserving their in vivo phenotype, requires biosensors of exquisite constitution. Here we provide the methodology for utilizing the single wavelength cGMP-biosensor δ-FlincG in aortic vascular smooth muscle cells.
authors.affiliation: Department of Pharmacology, Yale University, New Haven, USA.
authors.forename: Kara F
authors.initials: KF
authors.lastname: Held
authors.forename: Wolfgang R
authors.initials: WR
authors.lastname: Dostmann
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D002469:Cell Separation / Q000379:methods; D006152:Cyclic GMP / Q000737:chemistry / Q000378:metabolism*; D005822:Genetic Vectors / Q000235:genetics; D007091:Image Processing, Computer-Assisted / Q000379:methods; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000378:metabolism*; D051381:Rats; D014161:Transduction, Genetic
chemical_list: D006152:Cyclic GMP