title: [Intervention effect of aqueous fractions from Boschniakia rossica on hepatic oxidative stress in mice with liver injury induced by carbon tetrachloride].
abstract: OBJECTIVE
  To investigate the intervention effect of aqueous fractions from Boschniakia rossica (BRAF) on hepatic oxidative stress in mice with liver injury induced by carbon tetrachloride (CCl4).
  
  
  METHODS
  The experimental mice were randomly assigned into the normal control group, the model group, the silymarin (positive control) group, as well as high and low dose BRAF groups. Mice were treated intragastrically with silymarin or BRAF once every day for 7 days. At the end of the experiment, CCl4 was injected intraperitoneally into the mice to establish the acute liver injury model. The pathological changes was detected with hematoxylin and eosin (HE) staining, and the activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), superoxide dismutase (SOD) , catalase (CAT), glutathione peroxidase (GPx), Na+ -K+ -ATPase, Ca2+ -Mg2+ -ATPase, and the contents of reduced glutathione (GSH) and malondialdehyde (MDA) were detected by the colorimetric method.
  
  
  RESULTS
  BRAF significantly reduced ALT, AST and ALP activities in serum, alleviated hepatic injury induced by CCl4, increased SOD, CAT, GPx and GSH levels in liver, and SOD, Na + -K + -ATPase and Ca2+ -Mg2 + -ATPase activities in liver mitochondria, and decreased the MDA content in liver and liver mitochondria.
  
  
  CONCLUSIONS
  BRAF reduces hepatic oxidative stress in mice with acute liver injury induced by CCl4, thereby showing the protective effect on mice with acute liver injury induced by CCl4.
authors.affiliation: Medical College of Yanbian University, Yanji.133000, China.
authors.forename: Wen-Xi
authors.initials: WX
authors.lastname: Zhao
authors.forename: Mei-Hua
authors.initials: MH
authors.lastname: Jin
authors.forename: Tian
authors.initials: T
authors.lastname: Li
authors.forename: Yu-Jiao
authors.initials: YJ
authors.lastname: Wang
authors.forename: Ji-Shu
authors.initials: JS
authors.lastname: Quan
mesh_terms: D000818:Animals; D002251:Carbon Tetrachloride / Q000633:toxicity*; D056486:Chemical and Drug Induced Liver Injury / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D031670:Orobanchaceae / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects*; D012995:Solubility; D014867:Water / Q000737:chemistry*
chemical_list: D004365:Drugs, Chinese Herbal; D014867:Water; D002251:Carbon Tetrachloride