title: Effects of salvianolic acid and notoginseng triterpenes on angiogenesis in EA-hy926 cells in vitro.
abstract: OBJECTIVE
  To investigate the different effects of salvianolic acid and notoginseng triterpenes on proliferation, angiogenesis and expression of vascular endothelial growth factor in EA-hy926 cells in vitro.
  
  
  METHODS
  EA-hy926 cells were cultured in vitro. Salvianolic acid and notoginseng triterpenes at concentrations of 0.4, 0.8 and 1.2 mg·L(-1) were used to culture EA-hy926 cells. EA-hy926 cells in a blank control group were grown in culture solution only. Viability of cells was assessed by CCK-8, and after treated for 12 h, capillary-like structures were examined. After 24 h culture, the expression of VEGF was detected by real-time PCR.
  
  
  RESULTS
  Salvianolic acid at 0.4, 0.8 mg·L(-1), the same as notoginseng triterpenes, increased VEGF content in EA-hy926 cells. Expression of VEGF protein in the salvianolic acid at 1.2 mg·L(-1) group, was up-regulated as compared with notoginseng triterpenes group (P < 0.05).
  
  
  CONCLUSIONS
  Salvianolic acid and notoginseng triterpenes can promote EA-hy926 cell proliferation, angiogenesis and expression of VEGF protein. This analysis also provided evidence that salvianolic acid had the better effects as compared with notoginseng triterpenes.
authors.affiliation: Nanjing Medical University, Nanjing, China.
authors.forename: Hai-Bo
authors.initials: HB
authors.lastname: Shi
authors.forename: Jian-Dong
authors.initials: JD
authors.lastname: Chen
authors.forename: Xiao-Hu
authors.initials: XH
authors.lastname: Chen
authors.forename: Yun
authors.initials: Y
authors.lastname: He
authors.forename: Zhi-Jian
authors.initials: ZJ
authors.lastname: Yang
mesh_terms: D000475:Alkenes / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D023921:Coronary Stenosis / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D009389:Neovascularization, Pathologic / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D053723:Panax notoginseng / Q000737:chemistry*; D059808:Polyphenols / Q000494:pharmacology*; D014315:Triterpenes / Q000494:pharmacology*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: D000475:Alkenes; D004365:Drugs, Chinese Herbal; D059808:Polyphenols; D014315:Triterpenes; D042461:Vascular Endothelial Growth Factor A; C568740:salvianolic acid