title: Synthesis of triazole-linked oligonucleotides with high affinity to DNA complements and an analysis of their compatibility with biosystems.
abstract: New oligonucleotide analogues with triazole internucleotide linkages were synthesized, and their hybridization properties were studied. The analogues demonstrated DNA binding affinities similar to those of unmodified oligonucleotides. The modification was shown to protect the oligonucleotides from nuclease hydrolysis. The modified oligonucleotides were tested as PCR primers. Modifications remote from the 3'-terminus were tolerated by polymerases. Our results suggest that these new oligonucleotide analogues are among the most promising triazole DNA mimics characterized to date.
authors.affiliation: Institute for Physical-Chemical Medicine of Ministry of Public Health, Malaya Pirogovskaya str. 1a, 117312 Moscow, Russia. annavarizhuk@gmail.com
authors.forename: Anna M
authors.initials: AM
authors.lastname: Varizhuk
authors.forename: Dmitry N
authors.initials: DN
authors.lastname: Kaluzhny
authors.forename: Roman A
authors.initials: RA
authors.lastname: Novikov
authors.forename: Alexandr O
authors.initials: AO
authors.lastname: Chizhov
authors.forename: Igor P
authors.initials: IP
authors.lastname: Smirnov
authors.forename: Andrey N
authors.initials: AN
authors.lastname: Chuvilin
authors.forename: Olga N
authors.initials: ON
authors.lastname: Tatarinova
authors.forename: Gleb Y
authors.initials: GY
authors.lastname: Fisunov
authors.forename: Galina E
authors.initials: GE
authors.lastname: Pozmogova
authors.forename: Vladimir L
authors.initials: VL
authors.lastname: Florentiev
mesh_terms: D004247:DNA / Q000737:chemistry*; D003851:Deoxyribonucleases / Q000737:chemistry; D006868:Hydrolysis; D018716:Molecular Mimicry; D015335:Molecular Probes / Q000138:chemical synthesis*; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D014230:Triazoles / Q000737:chemistry*
chemical_list: D015335:Molecular Probes; D009841:Oligonucleotides; D014230:Triazoles; D004247:DNA; D003851:Deoxyribonucleases