title: Prevalence of ESBL producing Escherichia Coli and Klebsiella species with their co-resistance pattern to antimicrobials.
abstract: Extended spectrum β-lactamase producing bacteria are potential emerging pathogens and continue to be a major challenge in clinical setup worldwide. In the present study an attempt was made to study the prevalence of extended spectrum β-lactamase producing Escherichia coli and Klebsiella species from clinical isolates in a rural tertiary care hospital in West Bengal, India with their antimicrobial susceptibility as well as co-resistance pattern to different antimicrobials. A total of 179 Escherichia coli and 62 Klebsiella isolates recovered from various clinical samples of urine, pus, aural swabs and respiratory secretions (including sputum) for a period of six months were subjected to routine antimicrobial susceptibility testing and also tested for extended spectrum β-lactamase production as per NCCLS recommendations. Extended spectrum β-lactamase was detected in 32.40% of Escherichia coli and 40.32% of Klebsiella species isolates. Urine, pus and respiratory samples were common source of extended spectrum β-lactamase producers and resistance rate of these organisms to third generation cephalosporins were more than 30 to 40%. Co-resistance pattern of these extended spectrum β-lactamase producers to other commonly used antimicrobials were also statistically significant (p≤0.05). From the study it is concluded that indiscriminate use of third generation cephalosporins may be responsible for the selection of extended spectrum β-lactamase producing multidrug resistant strains in hospital setup and amikacin is a reliable drug against them.
authors.affiliation: Department of Microbiology, Burdwan Medical College, Burdwan, West Bengal, India.
authors.forename: T
authors.initials: T
authors.lastname: Biswas
authors.forename: M
authors.initials: M
authors.lastname: Das
authors.forename: R
authors.initials: R
authors.lastname: Mondal
authors.forename: H J
authors.initials: HJ
authors.lastname: Raj
authors.forename: S
authors.initials: S
authors.lastname: Mondal
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003430:Cross-Sectional Studies; D004926:Escherichia coli / Q000187:drug effects* / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D007709:Klebsiella / Q000187:drug effects* / Q000302:isolation & purification / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D015995:Prevalence; D011446:Prospective Studies; D001618:beta-Lactamases / Q000096:biosynthesis*
chemical_list: D000900:Anti-Bacterial Agents; D001618:beta-Lactamases