title: A microfluidic chip-based fluorescent biosensor for the sensitive and specific detection of label-free single-base mismatch via magnetic beads-based "sandwich" hybridization strategy.
abstract: A novel microfluidic chip-based fluorescent DNA biosensor, which utilized the electrophoretic driving mode and magnetic beads-based "sandwich" hybridization strategy, was developed for the sensitive and ultra-specific detection of single-base mismatch DNA in this study. In comparison with previous biosensors, the proposed DNA biosensor has much more robust resistibility to the complex matrix of real saliva and serum samples, shorter analysis time, and much higher discrimination ability for the detection of single-base mismatch. These features, as well as its easiness of fabrication, operation convenience, stability, better reusability, and low cost, make it a promising alternative to the SNPs genotyping/detection in clinical diagnosis. By using the biosensor, we have successfully determined oral cancer-related DNA in saliva and serum samples without sample labeling and any preseparation or dilution with a detection limit of 5.6 × 10(-11) M, a RSD (n = 5) < 5% and a discrimination factor of 3.58-4.54 for one-base mismatch.
authors.affiliation: Key Lab of Analysis and Detection for Food Safety of Ministry of Education, Fujian Provincial Key Lab of Analysis and Detection for Food Safety, Department of Chemistry Fuzhou University, Fuzhou, Fujian, PR China.
authors.forename: ZongWen
authors.initials: Z
authors.lastname: Wang
authors.forename: YingWei
authors.initials: Y
authors.lastname: Fan
authors.forename: JinFa
authors.initials: J
authors.lastname: Chen
authors.forename: Ying
authors.initials: Y
authors.lastname: Guo
authors.forename: WeiHua
authors.initials: W
authors.lastname: Wu
authors.forename: Ye
authors.initials: Y
authors.lastname: He
authors.forename: LiangJun
authors.initials: L
authors.lastname: Xu
authors.forename: FengFu
authors.initials: F
authors.lastname: Fu
mesh_terms: D020137:Base Pair Mismatch; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000097:blood / Q000235:genetics; D005819:Genetic Markers; D005838:Genotype; D006801:Humans; D016014:Linear Models; D059346:Magnets; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D008957:Models, Genetic; D009062:Mouth Neoplasms / Q000235:genetics; D009693:Nucleic Acid Hybridization / Q000379:methods*; D012463:Saliva / Q000737:chemistry; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
chemical_list: D005819:Genetic Markers; D004247:DNA
keywords: Biosensor; Magnetic beads; Microfluidic chip; Oral cancer; Single nucleotide polymorphism