title: Functional dissection and module swapping of fungal cyclooligomer depsipeptide synthetases.
abstract: BbBSLS and BbBEAS were dissected and reconstituted in Saccharomyces cerevisiae. The intermodular linker is essential for the reconstitution of the separate modules. Module 1 can be swapped between BbBEAS and BbBSLS, while modules 2 and 3 control the product profiles. BbBSLS is a flexible enzyme that also synthesizes beauvericins.
authors.affiliation: Department of Applied Chemistry and Biological Engineering, College of Chemical Engineering, Northeast Dianli University, Jilin, Jilin 132012, China.
authors.forename: Dayu
authors.initials: D
authors.lastname: Yu
authors.forename: Fuchao
authors.initials: F
authors.lastname: Xu
authors.forename: David
authors.initials: D
authors.lastname: Gage
authors.forename: Jixun
authors.initials: J
authors.lastname: Zhan
mesh_terms: D000970:Antineoplastic Agents / Q000378:metabolism; D047630:Depsipeptides / Q000096:biosynthesis*; D010453:Peptide Synthases / Q000096:biosynthesis*; D010456:Peptides, Cyclic / Q000096:biosynthesis*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
chemical_list: D000970:Antineoplastic Agents; D047630:Depsipeptides; D010456:Peptides, Cyclic; C004456:beauvericin; C035050:bassianolide; D010453:Peptide Synthases; C055861:beauvericin synthetase