title: A novel ameloblastoma cell line (AM-3) secretes MMP-9 in response to Wnt-3a and induces osteoclastogenesis.
abstract: OBJECTIVE
  Ameloblastoma has a high risk of bone invasion and local recurrence. However, the mechanisms of bone invasion in ameloblastoma remain unclear. In this study, we established an experimental model for matrix metalloproteinase (MMP) induction and osteoclastogenesis using ameloblastoma-derived cells.
  
  
  METHODS
  We established an ameloblastoma-derived cell line without viral genes and analyzed the expression of all Wnt and Frizzled members and MMPs by real-time reverse transcription-polymerase chain reaction, and analyzed the activity of MMP-2 and MMP-9 by the in-gel-gelatinase assay.
  
  
  RESULTS
  AM-3, newly established ameloblastoma-derived cells retained the morphology of primary-cultured ameloblastoma cells. AM-3 cells overexpressed the messenger RNA of Wnt-5a, Frizzled-2, MMP-2, and MMP-9 and showed the potential of osteoclastogenesis. In addition, Wnt-3a-treatment induced expression and activation of MMP-9 in AM-3 cells.
  
  
  CONCLUSIONS
  Our study suggests that AM-3 cells retained the characteristics of ameloblastoma, without acquiring typical features of cancer cells. Furthermore, Wnt signaling induced MMP-9 in ameloblastoma cells.
authors.affiliation: Department of Biochemistry and Genetics, Kagoshima University Graduate School of Medical and Dental Sciences, Japan.
authors.forename: Toshiro
authors.initials: T
authors.lastname: Kibe
authors.forename: Takao
authors.initials: T
authors.lastname: Fuchigami
authors.forename: Michiko
authors.initials: M
authors.lastname: Kishida
authors.forename: Mikio
authors.initials: M
authors.lastname: Iijima
authors.forename: Kiyohide
authors.initials: K
authors.lastname: Ishihata
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Hijioka
authors.forename: Akihiko
authors.initials: A
authors.lastname: Miyawaki
authors.forename: Ichiro
authors.initials: I
authors.lastname: Semba
authors.forename: Norifumi
authors.initials: N
authors.lastname: Nakamura
authors.forename: Tohru
authors.initials: T
authors.lastname: Kiyono
authors.forename: Shosei
authors.initials: S
authors.lastname: Kishida
mesh_terms: D000564:Ameloblastoma / Q000378:metabolism*; D045744:Cell Line, Tumor / Q000378:metabolism; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics / Q000502:physiology*; D051157:Frizzled Receptors / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D006801:Humans; D007573:Jaw Neoplasms / Q000235:genetics / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D010010:Osteoclasts / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D060449:Wnt Signaling Pathway / Q000502:physiology*
chemical_list: D051157:Frizzled Receptors; D012333:RNA, Messenger; D020780:Matrix Metalloproteinase 9