title: Phylogenetic position and virulence apparatus of the pear flower necrosis pathogen Erwinia piriflorinigrans CFBP 5888T as assessed by comparative genomics.
abstract: Erwinia piriflorinigrans is a necrotrophic pathogen of pear reported from Spain that destroys flowers but does not progress further into the host. We sequenced the complete genome of the type strain CFBP 5888(T) clarifying its phylogenetic position within the genus Erwinia, and indicating a position between its closest relative, the epiphyte Erwinia tasmaniensis and other plant pathogenic Erwinia spp. (i.e., the fire blight pathogen E. amylovora and the Asian pear pathogen E. pyrifoliae). Common features are the type III and type VI secretion systems, amylovoran biosynthesis and desferrioxamine production. The E. piriflorinigrans genome also provided the first evidence for production of the siderophore chrysobactin within the genus Erwinia sensu stricto, which up to now was mostly associated with phytopathogenic, soft-rot Dickeya and Pectobacterium species. Plasmid pEPIR37, reported in this strain, is closely related to small plasmids found in the fire blight pathogen E. amylovora and E. pyrifoliae. The genome of E. piriflorinigrans also gives detailed insights in evolutionary genomics of pathoadapted Erwinia.
authors.affiliation: Agroscope Changins-Wädenswil ACW, Plant Protection Division, CH-8820 Wädenswil, Switzerland. Electronic address: theo.smits@acw.admin.ch.
authors.forename: Theo H M
authors.initials: TH
authors.lastname: Smits
authors.forename: Fabio
authors.initials: F
authors.lastname: Rezzonico
authors.forename: María M
authors.initials: MM
authors.lastname: López
authors.forename: Jochen
authors.initials: J
authors.lastname: Blom
authors.forename: Alexander
authors.initials: A
authors.lastname: Goesmann
authors.forename: Jürg E
authors.initials: JE
authors.lastname: Frey
authors.forename: Brion
authors.initials: B
authors.lastname: Duffy
mesh_terms: D001483:Base Sequence; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry* / Q000235:genetics*; D004885:Erwinia / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D035264:Flowers / Q000382:microbiology; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology; D010957:Plasmids; D031989:Pyrus / Q000382:microbiology; D017422:Sequence Analysis, DNA; D013030:Spain; D037521:Virulence Factors / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D037521:Virulence Factors
keywords: Bacterial evolution; CRISPR; CRISPR repeat region; CRR; EPS; Fire blight; ICE; NRPS/PKS; Pathoadaptation; Phylogeny; Rosaceae; T1SS; T2SS; T3SS; T6SS; Virulence; clustered regularly interspaced short palindromic repeats; exopolysaccharide; integrative conjugative element; non-ribosomal peptide synthases/polyketide synthases; type I secretion system; type II secretion system; type III secretion system; type VI secretion system