title: Directed self-assembly of microscale hydrogels by electrostatic interaction.
abstract: The unique benefit of electrostatic self-assembly of microscale components in solution is demonstrated for the first time. In particular, positive and negative treatment of poly(ethylene glycol) (PEG) facilitates a novel bottom-up assembly approach using electrostatic interaction from microgels with opposite charges. Fundamental investigations of electrostatic interaction of microgels reveal that the contact area of microgels determines the total energy of construct and thus the final patterns. The electrostatic self-assembly approach enables the fabrication of large and complex biological related structures (e.g., multi-layer spheroid) with accurate control. By the design of the microgels, the thickness and number of microgels in each layer can be controlled. Biological investigations of positive and negative treatments of PEG further prove the possibility of using this approach in tissue engineering, regenerative medicine and drug delivery.
authors.affiliation: The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Xi'an Jiaotong University School of Life Science and Technology, Xi'an 710049, People's Republic of China.
authors.forename: Yu Long
authors.initials: YL
authors.lastname: Han
authors.forename: Yanshen
authors.initials: Y
authors.lastname: Yang
authors.forename: Shaobao
authors.initials: S
authors.lastname: Liu
authors.forename: Jinhui
authors.initials: J
authors.lastname: Wu
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Chen
authors.forename: Tian Jian
authors.initials: TJ
authors.lastname: Lu
authors.forename: Feng
authors.initials: F
authors.lastname: Xu
mesh_terms: D000818:Animals; D002470:Cell Survival; D020100:Hydrogels / Q000138:chemical synthesis / Q000737:chemistry*; D051379:Mice; D041681:NIH 3T3 Cells; D055672:Static Electricity; D023822:Tissue Engineering / Q000295:instrumentation*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D020100:Hydrogels