title: Clickable tyrosine binding bifunctional linkers for preparation of DNA-protein conjugates.
abstract: We have prepared bifunctional linkers containing clickable functional groups that enable preparation of protein-DNA conjugates through binding onto tyrosine residues. Mild conjugation strategy was demonstrated using two proteins, streptavidin(STV) and myoglobin (Mb) and it resulted in conjugates with preserved functionality of both the proteins and DNA strands. Furthermore, we show that protein-DNA conjugates can be successfully immobilized onto solid surface containing complementary DNA strands and the enzymatic activity of Mb-DNA conjugates is even higher than that of corresponding conjugates prepared through Lys binding.
authors.affiliation: Karlsruher Institute of Technology (KIT), DFG-Center for Functional Nanostructures (CFN), Wolfgang-Gaede-Str. 1a, 76131 Karlsruhe, Germany.
authors.forename: Dennis M
authors.initials: DM
authors.lastname: Bauer
authors.forename: Ishtiaq
authors.initials: I
authors.lastname: Ahmed
authors.forename: Antonina
authors.initials: A
authors.lastname: Vigovskaya
authors.forename: Ljiljana
authors.initials: L
authors.lastname: Fruk
mesh_terms: D001665:Binding Sites; D057930:Click Chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry*; D015394:Molecular Structure; D009211:Myoglobin / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry*; D014443:Tyrosine / Q000737:chemistry*
chemical_list: D004277:DNA, Single-Stranded; D009211:Myoglobin; D014443:Tyrosine; D019809:Streptavidin