title: Optimization of bacteriocin production by Lactobacillus sp. MSU3IR against shrimp bacterial pathogens.
abstract: BACKGROUND
  Aquaculture is one amongst the growing and major food producing sectors. Shrimp culture is one of the subsectors of aquaculture that attracts more attention because of the economic interest. However, the shrimp culture systems have been facing severe consequences and economical losses due to disease outbreaks. Risk of disease outbreak can be combated with the application of probiotics. For economically viable production of such probiotic products, the present study provides information on the optimization and partial purification of bacteriocin produced by a goat milk isolate Lactobacillus sp. MSU3IR against the shrimp bacterial pathogens.
  
  
  RESULTS
  Bacteriocin production was estimated as a measure of bactericidal activity (arbitrary Unit/ml) over the test strains. The optimum culture conditions and media components for maximum bacteriocin production by Lactobacillus sp. MSU3IR were: pH: 5.0, temperature: 30°C, carbon source: lactose; nitrogen source: ammonium acetate; NaCl: 3.0% and surfactant: Tween 80. MRS medium was found to extend better bacteriocin production than other tested media. Upon partial purification of bacteriocin, the SDS-PAGE analysis had manifested the presence of two peptide bands with the molecular weight of 39.26 and 6.38 kDa, respectively.
  
  
  CONCLUSIONS
  The present results provide baseline trend for the statistical optimization, scale up process and efficient production of bacteriocin by the candidate bacterial strain Lactobacillus sp. MSU3IR which could be used to replace the usage of conventional chemotherapeutics in shrimp culture systems.
authors.affiliation: CAS in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai - 608 502, Tamilnadu, India. bioiypps@yahoo.co.in.
authors.forename: Palanisamy
authors.initials: P
authors.lastname: Iyapparaj
authors.forename: Thirumalai
authors.initials: T
authors.lastname: Maruthiah
authors.forename: Ramasamy
authors.initials: R
authors.lastname: Ramasubburayan
authors.forename: Santhiyagu
authors.initials: S
authors.lastname: Prakash
authors.forename: Chandrasekaran
authors.initials: C
authors.lastname: Kumar
authors.forename: Grasian
authors.initials: G
authors.lastname: Immanuel
authors.forename: Arunachalam
authors.initials: A
authors.lastname: Palavesam