title: Exogenous normal lymph alleviates lipopolysaccharide-induced acute kidney injury in rats.
abstract: BACKGROUND
  Acute kidney injury (AKI) is a common pathological process which occurs in hemorrhage, intoxication, etc. It has been shown that the lymphatic circulation plays an important regulatory role in the pathogenesis of hemorrhage shock, and that exogenous normal lymph (ENL) has a beneficial effect on multiple organ injuries. In the present study, we investigated the effect of ENL on lipopolysaccharide (LPS)-induced AKI in rats.
  
  
  METHODS
  The AKI was induced by the jugular vein injection of LPS (iv, 15 mg/kg). After 15 min of LPS injection, saline or ENL without cell components (5 mL/kg) was iv infused at the speed of 0.5 mL per minute. Then, the renal function indices in plasma and renal histomorphology, and the levels of P-selectin, intercellular adhesion molecule-1 (ICAM-1), myeloperoxidase (MPO) and Na(+)-K(+)-ATPase in renal tissue were assessed at 3 or 6 h after LPS injection.
  
  
  RESULTS
  LPS induced a severe kidney injury including increased levels of urea, creatinine in plasma, aggrandized activities of ICAM-1 and MPO in renal tissue, and decreased the Na(+)-K(+)-ATPase activity in renal cells. These deleterious effects of LPS were significantly ameliorated by ENL treatment.
  
  
  CONCLUSIONS
  The present results indicate that ENL protect against LPS-induced AKI, suggesting an alternative therapeutic strategy for treatment of kidney injury accompanied with severe infection or sepsis.
authors.affiliation: Institute of Microcirculation, Hebei North University, Zhangjiakou, Hebei, PR China.
authors.forename: Zi-Gang
authors.initials: ZG
authors.lastname: Zhao
authors.forename: Chun-Yu
authors.initials: CY
authors.lastname: Niu
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Zhang
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Rui
authors.initials: R
authors.lastname: Han
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Zhang
authors.forename: Ya-Li
authors.initials: YL
authors.lastname: Hou
mesh_terms: D058186:Acute Kidney Injury / Q000139:chemically induced / Q000473:pathology / Q000628:therapy*; D000818:Animals; D004285:Dogs; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D007677:Kidney Function Tests; D008070:Lipopolysaccharides; D008196:Lymph; D009929:Organ Size; D009195:Peroxidase / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
chemical_list: D008070:Lipopolysaccharides; D018799:Intercellular Adhesion Molecule-1; D009195:Peroxidase; D000254:Sodium-Potassium-Exchanging ATPase