title: Aging decreases antioxidant effects and increases lipid peroxidation in the Apolipoprotein E deficient mouse.
abstract: In this study, to study the effect of aging and Apolipoprotein E (ApoE) deficiency on antioxidant ability in mice, we examined whether lipid peroxidation is promoted by aging in ApoE deficient (ApoE(-/-)) mice, which have a shorter lifespan than normal mice. The levels of thiobarbituric acid-reactive substances (TBARS), a biomarker of lipid peroxidation, were measured in plasma and liver in ApoE(-/-) mice aged 12 weeks (young) and 52 weeks (early stage of senescence). TBARS in plasma and liver were significantly increased by aging. Next, we examined the reasons why lipid peroxidation was promoted by aging, based on measurement of protein and mRNA levels for antioxidant enzymes (superoxide dismutase, catalase, and glutathione peroxidase) in liver in ApoE(-/-) mice aged 12 and 52 weeks. The levels of superoxide dismutase 1 and 2 in liver were significantly decreased by aging. The mRNA level of catalase was also significantly decreased and the mRNA levels of superoxide dismutase 1, superoxide dismutase 2 and glutathione peroxidase 1 all showed a tendency to decrease with age. These results suggest that lipid peroxidation is caused by reduction of antioxidant activity with aging and that this promotes senescence and shortens lifespan in ApoE(-/-) mice.
authors.affiliation: Laboratory of Food and Biomolecular Science, Graduate School of Agriculture, Tohoku University, Sendai 981-8555, Japan.
authors.forename: Taro
authors.initials: T
authors.lastname: Honma
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Tsuduki
authors.forename: Soko
authors.initials: S
authors.lastname: Sugawara
authors.forename: Yasuna
authors.initials: Y
authors.lastname: Kitano
authors.forename: Junya
authors.initials: J
authors.lastname: Ito
authors.forename: Ryo
authors.initials: R
authors.lastname: Kijima
authors.forename: Mari
authors.initials: M
authors.lastname: Tsubata
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Nakagawa
authors.forename: Teruo
authors.initials: T
authors.lastname: Miyazawa
keywords: TBARS; aging; apolipoprotein E; lipid peroxidation; senescence