title: Dietary nickel chloride induces oxidative intestinal damage in broilers.
abstract: The purpose of this study was to investigate the oxidative damage induced by dietary nickel chloride (NiCl2) in the intestinal mucosa of different parts of the intestine of broilers, including duodenum, jejunum and ileum. A total of 240 one-day-old broilers were divided into four groups and fed on a corn-soybean basal diet as control diet or the same basal diet supplemented with 300, 600 or 900 mg/kg NiCl2 during a 42-day experimental period. The results showed that the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px), and the ability to inhibit hydroxy radical and glutathione (GSH) content were significantly (p < 0.05 or p < 0.01) decreased in the 300, 600 and 900 mg/kg groups in comparison with those of the control group. In contrast, malondialdehyde (MDA) content was significantly (p < 0.05 or p < 0.01) higher in the 300, 600 and 900 mg/kg groups than that in the control group. It was concluded that dietary NiCl2 in excess of 300 mg/kg could cause oxidative damage in the intestinal mucosa in broilers, which finally impaired the intestinal functions including absorptive function and mucosal immune function. The oxidative damage might be a main mechanism on the effects of NiCl2 on the intestinal health of broilers.
authors.affiliation: Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an, China. wubangyuan2008@163.com
authors.forename: Bangyuan
authors.initials: B
authors.lastname: Wu
authors.forename: Hengmin
authors.initials: H
authors.lastname: Cui
authors.forename: Xi
authors.initials: X
authors.lastname: Peng
authors.forename: Jing
authors.initials: J
authors.lastname: Fang
authors.forename: Zhicai
authors.initials: Z
authors.lastname: Zuo
authors.forename: Junliang
authors.initials: J
authors.lastname: Deng
authors.forename: Jianying
authors.initials: J
authors.lastname: Huang
mesh_terms: D000821:Animal Feed; D000818:Animals; D000831:Animals, Newborn; D002374:Catalase / Q000378:metabolism; D002645:Chickens; D019587:Dietary Supplements / Q000633:toxicity*; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D007410:Intestinal Diseases / Q000139:chemically induced* / Q000201:enzymology; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000201:enzymology; D008315:Malondialdehyde / Q000378:metabolism; D009532:Nickel / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D008315:Malondialdehyde; C022838:nickel chloride; D009532:Nickel; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione