title: Biocompatibility of functionalized designer self-assembling nanofiber scaffolds containing FRM motif for neural stem cells.
abstract: Peptide self-assembling scaffolds have been widely used in tissue engineering. Much work has been focused on modifying the self-assembling scaffolds with functional motifs for desired biological activities. We report here the development of a biological material designed specifically for neural tissue engineering (NTE). Using RADA-16 (AcN-RADARADARADARADA-CONH2) as a base scaffold, we synthesized a 31 amino acid peptide RADA-FRM (AcN-RADARADARADARADAGGSIDRVEPYSSTAQ-CONH2) containing the neural cell adhesion molecule (NCAM)-derived mimetic peptide FRM (SIDRVEPYSSTAQ), which could undergo self-assembly into a nanofiber scaffold. We tested the characterization of the nanofiber scaffold using atomic force microscopy (AFM) and accessed the rheological properties of FRM-containing nanofiber scaffold (FRM-NS). Then we examined its biocompatibility on neural stem cells (NSCs) from neonatal rats. Regrettably, we found that FRM-NS had no effect on differentiation of NSCs. However, we tested that FRM-NS was noncytotoxic. Furthermore, compared to pure RADA-16 scaffold, we found that the designer self-assembling peptide scaffold containing FRM motif could significantly promote NSCs proliferation and stimulate NSCs migration into the three-dimensional scaffold. Our results indicate that the novel designer peptide scaffold containing FRM had excellent biocompatibility with NSCs and may be useful for central nervous tissue repair.
authors.affiliation: Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China.
authors.forename: Zhenwei
authors.initials: Z
authors.lastname: Zou
authors.forename: Ting
authors.initials: T
authors.lastname: Liu
authors.forename: JingFeng
authors.initials: J
authors.lastname: Li
authors.forename: Pindong
authors.initials: P
authors.lastname: Li
authors.forename: Qian
authors.initials: Q
authors.lastname: Ding
authors.forename: Gang
authors.initials: G
authors.lastname: Peng
authors.forename: Qixin
authors.initials: Q
authors.lastname: Zheng
authors.forename: Xianlin
authors.initials: X
authors.lastname: Zeng
authors.forename: Yongchao
authors.initials: Y
authors.lastname: Wu
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Guo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D005453:Fluorescence; D020100:Hydrogels / Q000138:chemical synthesis; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D008969:Molecular Sequence Data; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D058953:Neural Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012212:Rheology / Q000187:drug effects; D018874:Spheroids, Cellular / Q000166:cytology / Q000187:drug effects; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D010455:Peptides
keywords: nerve tissue engineering; neural cell adhesion molecule; neural stem cell; scaffold; self assembly