title: Microscopic examination of gallbladder stones improves rate of detection of Clonorchis sinensis infection.
abstract: To improve the rate of detection of Clonorchis sinensis infection, we compared different specimens from patients with cholecystolithiasis. Feces, gallbladder bile, and gallbladder stones collected from 179 consecutive patients with cholecystolithiasis underwent microscopic examination, and according to the results, 30 egg-positive and 30 egg-negative fecal, gallbladder bile, and gallbladder stone specimens, respectively, underwent real-time fluorescent PCR. The detection rates of eggs in feces, bile, and gallbladder stones were 30.7%, 44.7%, and 69.8%, respectively, and the differences were statistically significant (P<0.01). The PCR results confirmed that the eggs in the specimens were C. sinensis eggs. Eggs in the feces were "fresh" and in the gallbladder stones were "old." Microscopic examination of gallbladder stones may improve the detection rates of C. sinensis infection, which is important for developing individualized treatments to prevent the recurrence of gallbladder stones and to prevent the occurrence of severe liver damage and cholangiocarcinoma.
authors.affiliation: Institute of Gallbladder Disease of Panyu, Guangzhou, People's Republic of China. fqj1958@163.com
authors.forename: Tie
authors.initials: T
authors.lastname: Qiao
authors.forename: Rui-hong
authors.initials: RH
authors.lastname: Ma
authors.forename: Xiao-bing
authors.initials: XB
authors.lastname: Luo
authors.forename: Pei-ming
authors.initials: PM
authors.lastname: Zheng
authors.forename: Zhen-liang
authors.initials: ZL
authors.lastname: Luo
authors.forename: Liu-qing
authors.initials: LQ
authors.lastname: Yang
mesh_terms: D000328:Adult; D000818:Animals; D002137:Calculi / Q000469:parasitology*; D019411:Clinical Laboratory Techniques / Q000379:methods*; D003003:Clonorchiasis / Q000175:diagnosis*; D003004:Clonorchis sinensis / Q000302:isolation & purification*; D005260:Female; D005704:Gallbladder / Q000469:parasitology*; D006801:Humans; D008297:Male; D008853:Microscopy; D008875:Middle Aged; D010274:Parasitology / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction