title: The DNA uptake ATPase PilF of Thermus thermophilus: a reexamination of the zinc content.
abstract: The DNA-translocator ATPase PilF of Thermus thermophilus HB27 is a hexamer built by six identical subunits. Despite the presence of a conserved zinc-binding site in every subunit, only one zinc atom per hexamer was found. Re-examination of the zinc content of PilF purified from cells grown in complex media with different lots of yeast extract revealed six zinc atoms per hexamer. These data demonstrate that the low zinc content reported before was most likely a result of zinc depletion of the yeast extract used.
authors.affiliation: Molecular Microbiology and Bioenergetics, Institute of Molecular Biosciences, Goethe University Frankfurt, Max-von-Laue-Str. 9, 60438 Frankfurt am Main, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Salzer
authors.forename: Martin
authors.initials: M
authors.lastname: Herzberg
authors.forename: Dietrich H
authors.initials: DH
authors.lastname: Nies
authors.forename: Goran
authors.initials: G
authors.lastname: Biuković
authors.forename: Gerhard
authors.initials: G
authors.lastname: Grüber
authors.forename: Volker
authors.initials: V
authors.lastname: Müller
authors.forename: Beate
authors.initials: B
authors.lastname: Averhoff
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D004247:DNA / Q000378:metabolism; D033701:Nucleobase, Nucleoside, Nucleotide, and Nucleic Acid Transport Proteins / Q000737:chemistry* / Q000378:metabolism; D021122:Protein Subunits / Q000737:chemistry / Q000378:metabolism; D016963:Thermus thermophilus / Q000201:enzymology*; D015032:Zinc / Q000032:analysis*
chemical_list: D001426:Bacterial Proteins; D033701:Nucleobase, Nucleoside, Nucleotide, and Nucleic Acid Transport Proteins; D021122:Protein Subunits; D004247:DNA; D015032:Zinc