title: Non-meiotic chromosome instability in human immature oocytes.
abstract: Aneuploidy has been a major issue in human gametes and is closely related to fertility problems, as it is known to be present in cleavage stage embryos and gestational losses. Pre-meiotic chromosome abnormalities in women have been previously described. The aim of this study is to assess the whole-chromosome complement in immature oocytes to find those abnormalities caused by mitotic instability. For this purpose, a total of 157 oocytes at the germinal vesicle or metaphase I stage, and discarded from IVF cycles, were analysed by CGH. Fifty-six women, between 18 and 45 years old (mean 32.5 years), including 32 IVF patients (25-45 years of age) and 24 IVF oocyte donors (18-33 years of age), were included in the study. A total of 25/157 (15.9%) of the oocytes analysed, obtained from three IVF clinics, contained chromosome abnormalities, including both aneuploidy (24/157) and structural aberrations (9/157). Independently of the maternal age, the incidence of abnormal oocytes which originated before meiosis is 15.9%, and these imbalances were found in 33.9% of the females studied. This work sheds light on the relevance of mitotic instability responsible for the generation of the abnormalities present in human oocytes.
authors.affiliation: 1] Càtedra de Recerca Eugin-UAB, Universitat Autònoma de Barcelona, Bellaterra, Spain [2] Unitat de Biologia Cellular i Genètica Mèdica Facultat de Medicina Departament de Biologia Cellular, Fisiologia i Immunologia Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors.forename: Gemma
authors.initials: G
authors.lastname: Daina
authors.affiliation: Unitat de Biologia Cellular i Genètica Mèdica Facultat de Medicina Departament de Biologia Cellular, Fisiologia i Immunologia Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors.forename: Laia
authors.initials: L
authors.lastname: Ramos
authors.affiliation: Unitat de Biologia Cellular i Genètica Mèdica Facultat de Medicina Departament de Biologia Cellular, Fisiologia i Immunologia Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors.forename: Mariona
authors.initials: M
authors.lastname: Rius
authors.affiliation: 1] Càtedra de Recerca Eugin-UAB, Universitat Autònoma de Barcelona, Bellaterra, Spain [2] Clínica Eugin, Barcelona, Spain.
authors.forename: Albert
authors.initials: A
authors.lastname: Obradors
authors.affiliation: 1] Càtedra de Recerca Eugin-UAB, Universitat Autònoma de Barcelona, Bellaterra, Spain [2] Unitat de Biologia Cellular i Genètica Mèdica Facultat de Medicina Departament de Biologia Cellular, Fisiologia i Immunologia Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Del Rey
authors.affiliation: Unitat de Biologia Cellular i Genètica Mèdica Facultat de Medicina Departament de Biologia Cellular, Fisiologia i Immunologia Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors.forename: Magda
authors.initials: M
authors.lastname: Giralt
authors.affiliation: Laboratori de Medicina Computacional, Unitat de Bioestadistica, Universitat Autònoma de Barcelona, Barcelona, Spain.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Campillo
authors.affiliation: Institut Marquès Servei de Biologia de la Reproducció, Barcelona, Spain.
authors.forename: Esther
authors.initials: E
authors.lastname: Velilla
authors.affiliation: Clínica Eugin, Barcelona, Spain.
authors.forename: Aïda
authors.initials: A
authors.lastname: Pujol
authors.affiliation: Fundació Puigvert Hospital de Sant Pau i de la Santa Creu, Barcelona, Spain.
authors.forename: Olga
authors.initials: O
authors.lastname: Martinez-Pasarell
authors.affiliation: 1] Càtedra de Recerca Eugin-UAB, Universitat Autònoma de Barcelona, Bellaterra, Spain [2] Unitat de Biologia Cellular i Genètica Mèdica Facultat de Medicina Departament de Biologia Cellular, Fisiologia i Immunologia Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors.forename: Jordi
authors.initials: J
authors.lastname: Benet
authors.affiliation: 1] Càtedra de Recerca Eugin-UAB, Universitat Autònoma de Barcelona, Bellaterra, Spain [2] Unitat de Biologia Cellular i Genètica Mèdica Facultat de Medicina Departament de Biologia Cellular, Fisiologia i Immunologia Universitat Autònoma de Barcelona, Bellaterra, Spain.
authors.forename: Joaquima
authors.initials: J
authors.lastname: Navarro
mesh_terms: D000293:Adolescent; D000328:Adult; D000782:Aneuploidy; D043171:Chromosomal Instability; D055028:Comparative Genomic Hybridization; D006801:Humans; D008540:Meiosis; D008875:Middle Aged; D009865:Oocytes / Q000473:pathology; D055815:Young Adult