title: Heterologous expression and functional characterization of avian mu-class glutathione S-transferases.
abstract: Hepatic glutathione S-transferases (GSTs: EC2.5.1.1.8) catalyze the detoxification of reactive electrophilic compounds, many of which are toxic and carcinogenic intermediates, via conjugation with the endogenous tripeptide glutathione (GSH). Glutathione S-transferase (GST)-mediated detoxification is a critical determinant of species susceptibility to the toxic and carcinogenic mycotoxin aflatoxin B1 (AFB1), which in resistant animals efficiently detoxifies the toxic intermediate produced by hepatic cytochrome P450 bioactivation, the exo-AFB1-8,9-epoxide (AFBO). Domestic turkeys (Meleagris gallopavo) are one of the most sensitive animals known to AFB1, a condition associated with a deficiency of hepatic GST-mediated detoxification of AFBO. We have recently shown that unlike their domestic counterparts, wild turkeys (Meleagris gallopavo silvestris), which are relatively resistant, express hepatic GST-mediated detoxification activity toward AFBO. Because of the importance of GSTs in species susceptibility, and to explore possible GST classes involved in AFB1 detoxification, we amplified, cloned, expressed and functionally characterized the hepatic mu-class GSTs tGSTM3 (GenBank accession no. JF340152), tGSTM4 (JF340153) from domestic turkeys, and a GSTM4 variant (ewGSTM4, JF340154) from Eastern wild turkeys. Predicted molecular masses of tGSTM3 and two tGSTM4 variants were 25.6 and 25.8kDa, respectively. Multiple sequence comparisons revealed four GSTM motifs and the mu-loop in both proteins. tGSTM4 has 89% amino acid sequence identity to chicken GSTM2, while tGSTM3 has 73% sequence identity to human GSTM3 (hGSTM3). Specific activities of Escherichia coli-expressed tGSTM3 toward 1-chloro-2,4-dinitrobenzene (CDNB) and peroxidase activity toward cumene hydroperoxide were five-fold greater than tGSTM4 while tGSTM4 possessed more than three-fold greater activity toward 1,2-dichloro-4-nitrobenzene (DCNB). The two enzymes displayed equal activity toward ethacrynic acid (ECA). However, none of the GSTM proteins had AFBO detoxification capability, in contrast to recombinant alpha-class GSTs shown in our recent study to possess this important activity. In total, our data indicate that although turkey hepatic GSTMs may contribute to xenobiotic detoxification, they probably play no role in detoxification of AFBO in the liver.
authors.affiliation: Graduate Toxicology Program, Department of Veterinary Sciences, Utah State University, Logan, UT 84322, USA.
authors.forename: Brett R
authors.initials: BR
authors.lastname: Bunderson
authors.forename: Ji Eun
authors.initials: JE
authors.lastname: Kim
authors.forename: Amanda
authors.initials: A
authors.lastname: Croasdell
authors.forename: Kristelle M
authors.initials: KM
authors.lastname: Mendoza
authors.forename: Kent M
authors.initials: KM
authors.lastname: Reed
authors.forename: Roger A
authors.initials: RA
authors.lastname: Coulombe
mesh_terms: D016604:Aflatoxin B1 / Q000378:metabolism* / Q000633:toxicity; D000595:Amino Acid Sequence; D000818:Animals; D001555:Benzene Derivatives / Q000378:metabolism; D004137:Dinitrochlorobenzene / Q000378:metabolism; D004976:Ethacrynic Acid / Q000378:metabolism; D005982:Glutathione Transferase / Q000096:biosynthesis* / Q000378:metabolism; D008658:Inactivation, Metabolic; D008099:Liver / Q000201:enzymology; D008297:Male; D008969:Molecular Sequence Data; D009578:Nitrobenzenes / Q000378:metabolism; D016415:Sequence Alignment; D013379:Substrate Specificity; D014422:Turkeys / Q000378:metabolism*
chemical_list: D001555:Benzene Derivatives; D004137:Dinitrochlorobenzene; D009578:Nitrobenzenes; C028328:1,2-dichloro-4-nitrobenzene; D016604:Aflatoxin B1; D005982:Glutathione Transferase; D004976:Ethacrynic Acid; C007164:cumene hydroperoxide