title: Fast mass spectrometry detection of tryptophan-containing peptides and proteins by reduction with pyridine-borane.
abstract: In this note, we describe a method devised to detect, by means of mass spectrometry (MS), tryptophan-containing peptides and proteins using pyridine-borane. This reagent selectively reduces tryptophan residues, converting them to 2,3-dihydro-tryptophan, thereby enabling quantitation of tryptophans.
authors.affiliation: Department of Biological Chemistry, Weizmann Institute of Science, 76100 Rehovot, Israel.
authors.forename: T
authors.initials: T
authors.lastname: Miron
authors.forename: M
authors.initials: M
authors.lastname: Wilchek
mesh_terms: D001896:Boron Compounds / Q000032:analysis* / Q000737:chemistry; D007202:Indicators and Reagents; D013058:Mass Spectrometry / Q000379:methods*; D010084:Oxidation-Reduction; D010455:Peptides / Q000032:analysis* / Q000737:chemistry; D011725:Pyridines / Q000032:analysis* / Q000737:chemistry; D014364:Tryptophan / Q000032:analysis* / Q000737:chemistry
chemical_list: D001896:Boron Compounds; D007202:Indicators and Reagents; D010455:Peptides; D011725:Pyridines; C041928:pyridine borane; D014364:Tryptophan
keywords: MS; Modification; Pyridine–borane; Tryptophan