title: Soluble human leukocyte antigen G molecule expression in allogeneic hematopoietic stem cell transplantation: good predictor of acute graft-versus-host disease.
abstract: BACKGROUND
  Graft-versus-host disease (GVHD) remains a main complication of allogeneic hematopoietic stem cell transplantation (allo-HSCT). Human leukocyte antigen G (HLA-G) is a non-classical class I molecule exerting multiple immunoregulatory functions. The aim of this study was to explore the relationship between soluble HLA-G (sHLA-G) and GVHD after allo-HSCT.
  
  
  METHODS
  The sHLA-G levels were examined using enzyme-linked immunosorbent assay in patients with hematological malignancies (n = 106) before transplantation, on days +15 and +30 after transplantation, as well as healthy volunteers (n = 10).
  
  
  RESULTS
  The levels of sHLA-G5, sHLA-G6 and sHLA-G7 in patients on days +15 and +30 after transplantation were all significantly higher than those before transplantation (all p ≤ 0.001). The increased levels of sHLA-G5 on days +15 and +30 after transplantation were both significantly higher in patients with grade 0-I acute GVHD (aGVHD) compared to those with grade II-IV aGVHD (both p < 0.001). The increased levels of sHLA-G5 on days +15 and +30 after transplantation were both negatively correlated with the severity of aGVHD (both p < 0.001).
  
  
  CONCLUSIONS
  sHLA-G5 might be a predictor of the occurrence and severity of aGVHD, which may help to establish individual prophylaxis against aGVHD and improve the survival for patients after allo-HSCT.
authors.affiliation: Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.forename: Yinkui
authors.initials: Y
authors.lastname: Chen
authors.forename: Li
authors.initials: L
authors.lastname: Xuan
authors.forename: Xiuli
authors.initials: X
authors.lastname: Wu
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Fan
authors.forename: Fen
authors.initials: F
authors.lastname: Huang
authors.forename: Xian
authors.initials: X
authors.lastname: Zhang
authors.forename: Qianli
authors.initials: Q
authors.lastname: Jiang
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.forename: Qifa
authors.initials: Q
authors.lastname: Liu
mesh_terms: D000293:Adolescent; D000328:Adult; D005260:Female; D005786:Gene Expression Regulation; D006086:Graft vs Host Disease / Q000097:blood* / Q000628:therapy; D059951:HLA-G Antigens / Q000097:blood*; D019337:Hematologic Neoplasms / Q000097:blood* / Q000628:therapy*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D013997:Time Factors; D014184:Transplantation, Homologous
chemical_list: D059951:HLA-G Antigens