title: Interaction of tannase from Aspergillus niger with polycations applied to its primary recovery.
abstract: The interaction of tannase (TAH) with chitosan, polyethyleneimine and Eudragit(®)E100 was studied. It was found that TAH selectively binds to these polycations (PC), probably due to the acid nature of the target protein. TAH could interact with these PC depending on the medium conditions. The effect of the interaction on the secondary and tertiary structure of TAH was assayed through circular dichroism and fluorescence spectroscopy. TAH was recovered from Aspergillus niger culture broth by means of precipitation and adsorption using chitosan.
authors.affiliation: Food Research Department, School of Chemistry, Universidad Autónoma de Coahuila, Blvd. Venustiano Carranza and J. Cárdenas s/n, ZIP 25280, Saltillo, Coahuila, Mexico.
authors.forename: Luis V Rodríguez
authors.initials: LV
authors.lastname: Durán
authors.forename: Darío
authors.initials: D
authors.lastname: Spelzini
authors.forename: Valeria
authors.initials: V
authors.lastname: Boeris
authors.forename: Cristóbal N
authors.initials: CN
authors.lastname: Aguilar
authors.forename: Guillermo A
authors.initials: GA
authors.lastname: Picó
mesh_terms: D000179:Acrylates / Q000737:chemistry* / Q000378:metabolism; D001234:Aspergillus niger / Q000201:enzymology*; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry* / Q000378:metabolism; D002412:Cations / Q000737:chemistry / Q000378:metabolism; D048271:Chitosan / Q000737:chemistry* / Q000378:metabolism; D002942:Circular Dichroism; D011094:Polyethyleneimine / Q000737:chemistry* / Q000378:metabolism; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D012965:Sodium Chloride / Q000737:chemistry; D013050:Spectrometry, Fluorescence
chemical_list: D000179:Acrylates; D002412:Cations; C477020:Eudragit E100; D011108:Polymers; D012965:Sodium Chloride; D011094:Polyethyleneimine; D048271:Chitosan; D002265:Carboxylic Ester Hydrolases; C022711:tannase