title: An olive oil-derived antioxidant mixture ameliorates the age-related decline of skeletal muscle function.
abstract: Age-related skeletal muscle decline is characterized by the modification of sarcolemma ion channels important to sustain fiber excitability and to prevent metabolic dysfunction. Also, calcium homeostasis and contractile function are impaired. In the aim to understand whether these modifications are related to oxidative damage and can be reverted by antioxidant treatment, we examined the effects of in vivo treatment with an waste water polyphenolic mixture (LACHI MIX HT) supplied by LACHIFARMA S.r.l. Italy containing hydroxytirosol (HT), gallic acid, and homovanillic acid on the skeletal muscles of 27-month-old rats. After 6-week treatment, we found an improvement of chloride ClC-1 channel conductance, pivotal for membrane electrical stability, and of ATP-dependent potassium channel activity, important in coupling excitability with fiber metabolism. Both of them were analyzed using electrophysiological techniques. The treatment also restored the resting cytosolic calcium concentration, the sarcoplasmic reticulum calcium release, and the mechanical threshold for contraction, an index of excitation-contraction coupling mechanism. Muscle weight and blood creatine kinase levels were preserved in LACHI MIX HT-treated aged rats. The antioxidant activity of LACHI MIX HT was confirmed by the reduction of malondialdehyde levels in the brain of the LACHI MIX HT-treated aged rats. In comparison, the administration of purified HT was less effective on all the parameters studied. Although muscle function was not completely recovered, the present study provides evidence of the beneficial effects of LACHI MIX HT, a natural compound, to ameliorate skeletal muscle functional decline due to aging-associated oxidative stress.
authors.affiliation: Section of Pharmacology, Department of Pharmacy & Drug Sciences, University of Bari "Aldo Moro", Via Orabona 4-campus, 70125, Bari, Italy, sabata.pierno@uniba.it.
authors.forename: Sabata
authors.initials: S
authors.lastname: Pierno
authors.forename: Domenico
authors.initials: D
authors.lastname: Tricarico
authors.forename: Antonella
authors.initials: A
authors.lastname: Liantonio
authors.forename: Antonietta
authors.initials: A
authors.lastname: Mele
authors.forename: Claudio
authors.initials: C
authors.lastname: Digennaro
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Rolland
authors.forename: Gianpatrizio
authors.initials: G
authors.lastname: Bianco
authors.forename: Luciano
authors.initials: L
authors.lastname: Villanova
authors.forename: Alessandro
authors.initials: A
authors.lastname: Merendino
authors.forename: Giulia Maria
authors.initials: GM
authors.lastname: Camerino
authors.forename: Annamaria
authors.initials: A
authors.lastname: De Luca
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Desaphy
authors.forename: Diana Conte
authors.initials: DC
authors.lastname: Camerino
mesh_terms: D000284:Administration, Oral; D000375:Aging / Q000187:drug effects*; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D001921:Brain / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D018118:Chloride Channels / Q000378:metabolism; D005707:Gallic Acid / Q000008:administration & dosage / Q000494:pharmacology; D006719:Homovanillic Acid / Q000008:administration & dosage / Q000494:pharmacology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D053580:Muscle Strength / Q000187:drug effects; D018482:Muscle, Skeletal / Q000187:drug effects*; D000069463:Olive Oil; D018408:Patch-Clamp Techniques; D010626:Phenylethyl Alcohol / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology; D010938:Plant Oils / Q000008:administration & dosage / Q000494:pharmacology*; D015221:Potassium Channels / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012508:Sarcolemma / Q000187:drug effects; D012519:Sarcoplasmic Reticulum / Q000378:metabolism
chemical_list: D000975:Antioxidants; C086321:CLC-1 channel; D018118:Chloride Channels; D000069463:Olive Oil; D010938:Plant Oils; D015221:Potassium Channels; C005975:3,4-dihydroxyphenylethanol; D008315:Malondialdehyde; D005707:Gallic Acid; D010626:Phenylethyl Alcohol; D002118:Calcium; D006719:Homovanillic Acid