title: Enhancement of IGF-2-induced neurite outgrowth by IGF-binding protein-2 and osteoglycin in SH-SY5Y human neuroblastoma cells.
abstract: A previous study using a mouse model of depression showed that chronic immobilization stress (CIS) reduces levels of insulin-like growth factor (IGF)-2, IGF binding protein 2 (IGFBP2), osteoglycin, and fibromodulin in the amygdala. Here, using human neuroblastoma cells, we tested whether these four proteins cooperatively modulate neuronal plasticity. We found that IGF-2 and IGFBP2 synergistically increased neurite outgrowth via enhanced early signaling through the IGF type 1 receptor. Furthermore, we found that osteoglycin, a small leucine-rich proteoglycan, significantly increased IGF-2/IGFPB2-induced neurite outgrowth, but fibromodulin had no effect. We also found that central amygdala neurons of CIS-induced depressive mouse showed a decreased total dendritic length. These findings suggest that CIS-responsive proteins modulate neuronal morphology during chronic stress.
authors.affiliation: Department of Anatomy and Neurobiology, Institute of Health Sciences, Medical Research Center for Neural Dysfunction, School of Medicine, Gyeongsang National University, Jinju, Republic of Korea.
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Jeong
authors.forename: Sujoeng
authors.initials: S
authors.lastname: Kim
authors.forename: Soonwoong
authors.initials: S
authors.lastname: Jung
authors.forename: Gyeongwha
authors.initials: G
authors.lastname: Kim
authors.forename: Hyeonwi
authors.initials: H
authors.lastname: Son
authors.forename: Dong Hoon
authors.initials: DH
authors.lastname: Lee
authors.forename: Gu Seob
authors.initials: GS
authors.lastname: Roh
authors.forename: Sang Soo
authors.initials: SS
authors.lastname: Kang
authors.forename: Gyeong Jae
authors.initials: GJ
authors.lastname: Cho
authors.forename: Wan Sung
authors.initials: WS
authors.lastname: Choi
authors.forename: Hyun Joon
authors.initials: HJ
authors.lastname: Kim
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D018971:Insulin-Like Growth Factor Binding Protein 2 / Q000378:metabolism*; D007335:Insulin-Like Growth Factor II / Q000378:metabolism*; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D016501:Neurites / Q000473:pathology*; D009447:Neuroblastoma / Q000473:pathology* / Q000503:physiopathology*; D009473:Neuronal Plasticity; D017526:Receptor, IGF Type 1 / Q000378:metabolism
chemical_list: D018971:Insulin-Like Growth Factor Binding Protein 2; D036341:Intercellular Signaling Peptides and Proteins; C063488:OGN protein, human; D007335:Insulin-Like Growth Factor II; D017526:Receptor, IGF Type 1
keywords: IGF-2; IGFBP2; Neurite outgrowth; Osteoglycin; SH-SY5Y