title: Expression and displaying of β-glucosidase from Streptomyces coelicolor A3 in Escherichia coli.
abstract: Two genes encoding β-glucosidase from Streptomyces coelicolor A3(2) were cloned and expressed in Escherichia coli BL21 (DE3). Two recombinant enzymes (SC1059 and SC7558) were purified and characterized. The molecular mass of the purified SC1059 and SC7558 as determined by SDS-PAGE agrees with the calculated values (51.0 and 52.2 kDa, respectively). Optimal temperature and pH for the two enzymes were both at 35 °C and 6.0. SC7558 exhibited to be much more active than SC1059 under optimal conditions, and it was recombined with ice nucleation protein which could anchor on the surface of the cell. The optimal temperature and pH of the recombinant cells were 55 °C and 8.0, respectively. The resultant cells were to be used as material for immobilized β-glucosidase, which is convenient to catalyze substrates in various complicated conditions.
authors.affiliation: Lab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Meilong Rd. 130, Shanghai 200237, China.
authors.forename: Ming-Zhu
authors.initials: MZ
authors.lastname: Gu
authors.forename: Jing-Chao
authors.initials: JC
authors.lastname: Wang
authors.forename: Wei-Bing
authors.initials: WB
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.forename: Bang-Ce
authors.initials: BC
authors.lastname: Ye
mesh_terms: D004789:Enzyme Activation; D004795:Enzyme Stability; D004926:Escherichia coli / Q000502:physiology*; D015202:Protein Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000096:biosynthesis*; D048372:Streptomyces coelicolor / Q000502:physiology*; D014162:Transfection / Q000379:methods*; D001617:beta-Glucosidase / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; D001617:beta-Glucosidase