title: Correlation with OCT and histology of photocoagulation lesions in patients and rabbits.
abstract: OBJECTIVE
  To examine spectral domain optical coherence tomographic (OCT) and histological images from comparable retinal photocoagulation lesions in rabbits, and to correlate these images with comparable OCT images from patients.
  
  
  METHODS
  508 rabbit lesions were examined by HE-stained paraffin histology. 1019 rabbit lesions versus 236 patient lesions were examined by OCT, all at the time-points 1 hr, 1 week and 4 weeks after photocoagulation. We analysed 100 μm lesions (in humans) and 133 μm lesions (in rabbits) of 200 ms exposures at powers titrated from the histological threshold up to intense damage. Lesions were matched according to morphological criteria.
  
  
  RESULTS
  Dome-shaped layer alterations, retinal infiltration by round, pigmented cells, outer nuclear layer interruption, and eventually full thickness retinal coagulation are detectable in histology and OCT. Horizontal damage extensions are found 1½ times larger in OCT. More intense irradiation was necessary to induce comparable layer affection in rabbit OCT as in histology. Restoration of the inner retinal layers is only shown in the OCT images. Comparable primary lesions caused more pronounced OCT changes in patients than in rabbits during healing.
  
  
  CONCLUSIONS
  Optical coherence tomographic images indicate different tissue changes than histologic images. After photocoagulation, they show wider horizontal damage diameters, but underestimate axial damage particularly during healing. Conclusions on retinal restoration should not be drawn from OCT findings alone. Retinal recovery after comparable initial lesions appears to be more complete in rabbit than in patient OCTs.
authors.affiliation: Department of Ophthalmology, University of Kiel, Kiel, GermanyInstitute of Biomedical Optics, University of Lübeck, Lübeck, GermanyMedical Laser Center Lübeck GmbH, Lübeck, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Koinzer
authors.forename: Mark
authors.initials: M
authors.lastname: Saeger
authors.forename: Carola
authors.initials: C
authors.lastname: Hesse
authors.forename: Lea
authors.initials: L
authors.lastname: Portz
authors.forename: Susanne
authors.initials: S
authors.lastname: Kleemann
authors.forename: Kerstin
authors.initials: K
authors.lastname: Schlott
authors.forename: Ralf
authors.initials: R
authors.lastname: Brinkmann
authors.forename: Johann
authors.initials: J
authors.lastname: Roider
mesh_terms: D000818:Animals; D003930:Diabetic Retinopathy / Q000473:pathology* / Q000601:surgery; D006801:Humans; D017075:Laser Coagulation; D011817:Rabbits; D012160:Retina / Q000473:pathology* / Q000601:surgery; D031300:Retinal Vasculitis / Q000473:pathology* / Q000601:surgery; D012170:Retinal Vein Occlusion / Q000473:pathology* / Q000601:surgery; D013223:Statistics as Topic; D041623:Tomography, Optical Coherence; D014945:Wound Healing
keywords: histology; laser; optical coherence tomography; photocoagulation; retina; retinal healing