title: Optimized extraction method for LC-MS determination of bisphenol A, melamine and di(2-ethylhexyl) phthalate in selected soft drinks, syringes, and milk powder.
abstract: Described below is an optimized solid-phase extraction method (SPE) for the simultaneous determination of three hazardous plastic additives. Two high-performance liquid chromatographic-electrospray ionization-mass spectrometric (HPLC-ESI-MS) methods were developed and validated to estimate the melamine (MEL), bis(2-ethylhexyl) phthalate (DEHP), and bisphenol A (BPA) contents in drinking water, syringes, soft drinks, and dry milk powder. One extraction procedure optimally recovered all three substances from the different matrices. Two extraction columns were combined and included a silica gel LiChroprep RP-2 column (20:1, g/g, top column) and a Sep-Pak with a C18 column (500mg, bottom column). The analytical column was an Agilent Eclipse XDB-C8 column, 4.6mm×150mm, 5μm, maintained at 50±2°C. MEL and DEHP were monitored by positive triple quad mass spectrometry (TQ-MS) using an acidic mobile phase, while BPA was monitored by negative TQ-MS using a mobile phase containing a 0.05% ammonia solution. The general linear range of the three compounds ranged from 12 to 1000pg/μL in the injected solution (25μL). The average extraction recoveries were within the range of 83.0-102.5%. Relatively high concentrations of BPA and DEHP were found in the milk powder and sterile syringes.
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, King Abdulaziz University, P.O. Box 80260, Jeddah 21589, Saudi Arabia. khedr61@gmail.com
authors.forename: Alaa
authors.initials: A
authors.lastname: Khedr
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000032:analysis*; D002253:Carbonated Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004051:Diethylhexyl Phthalate / Q000032:analysis*; D060766:Drinking Water / Q000737:chemistry; D016014:Linear Models; D008892:Milk / Q000737:chemistry; D010636:Phenols / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013594:Syringes; D014227:Triazines / Q000032:analysis*; D014874:Water Pollutants, Chemical / Q000032:analysis; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D004051:Diethylhexyl Phthalate; D060766:Drinking Water; D010636:Phenols; D014227:Triazines; D014874:Water Pollutants, Chemical; C006780:bisphenol A; D000098826:Bisphenol A Compounds; C011907:melamine