title: mRNA on the move: the road to its biological destiny.
abstract: Cells have evolved to regulate the asymmetric distribution of specific mRNA targets to institute spatial and temporal control over gene expression. Over the last few decades, evidence has mounted as to the importance of localization elements in the mRNA sequence and their respective RNA-binding proteins. Live imaging methodologies have shown mechanistic details of this phenomenon. In this minireview, we focus on the advanced biochemical and cell imaging techniques used to tweeze out the finer aspects of mechanisms of mRNA movement.
authors.affiliation: Department of Anatomy and Structural Biology, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York 10461, USA.
authors.forename: Carolina
authors.initials: C
authors.lastname: Eliscovich
authors.forename: Adina R
authors.initials: AR
authors.lastname: Buxbaum
authors.forename: Zachary B
authors.initials: ZB
authors.lastname: Katz
authors.forename: Robert H
authors.initials: RH
authors.lastname: Singer
mesh_terms: D000818:Animals; D003593:Cytoplasm / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D017403:In Situ Hybridization; D008856:Microscopy, Fluorescence; D008957:Models, Genetic; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism*; D034443:RNA Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D012322:RNA Precursors; D012333:RNA, Messenger; D016601:RNA-Binding Proteins
keywords: 3′-UTR; Fluorescence; Gene Expression; In Vivo Imaging; Localization Element/Zipcode; RNA Transport; RNA-Protein Interaction; RNA-binding Proteins; mRNA; β-Actin mRNA