title: Induction of cross-reactive antibodies to novel H7N9 influenza virus by recombinant Newcastle disease virus expressing a North American lineage H7 subtype hemagglutinin.
abstract: Severe human disease caused by the emerging H7N9 influenza virus in China warrants a rapid response. Here, we present a recombinant Newcastle disease virus expressing a North American lineage H7 influenza virus hemagglutinin. Sera from immunized mice were cross-reactive to a broad range of H7 subtype viruses and inhibited hemagglutination by the novel H7 hemagglutinin. Immunized mice were protected against a heterologous H7 subtype challenge, and genetic analysis suggested that cross-protective antibodies recognize conserved antigenic sites.
authors.affiliation: Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
authors.forename: Peter H
authors.initials: PH
authors.lastname: Goff
authors.forename: Florian
authors.initials: F
authors.lastname: Krammer
authors.forename: Rong
authors.initials: R
authors.lastname: Hai
authors.forename: Christopher W
authors.initials: CW
authors.lastname: Seibert
authors.forename: Irina
authors.initials: I
authors.lastname: Margine
authors.forename: Adolfo
authors.initials: A
authors.lastname: García-Sastre
authors.forename: Peter
authors.initials: P
authors.lastname: Palese
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology*; D015153:Blotting, Western; D002681:China; D016000:Cluster Analysis; D021821:Communicable Diseases, Emerging / Q000276:immunology* / Q000517:prevention & control; D017124:Conserved Sequence / Q000276:immunology; D003429:Cross Reactions / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005822:Genetic Vectors / Q000235:genetics; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D009980:Influenza A virus / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008957:Models, Genetic; D009522:Newcastle disease virus / Q000276:immunology* / Q000378:metabolism; D009976:Orthomyxoviridae Infections / Q000276:immunology* / Q000517:prevention & control; D010802:Phylogeny; D059386:Reverse Genetics / Q000379:methods; D014765:Viral Vaccines / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007106:Immune Sera; D014765:Viral Vaccines; C109963:hemagglutinin, avian influenza A virus