title: The use of hormones indicators in human saliva in diagnosing parodontitis in pregnant women.
abstract: OBJECTIVE
  The purpose of this work- was to study the dynamics of biochemical parameters of human saliva and analyze the features of the chemical composition of the saliva of women with abnormal pregnancy and in periodontitis against pregnancy.
  
  
  METHODS
  THE STUDY INCLUDED FOUR GROUPS OF WOMEN: a control group of nonpregnant women of childbearing age (10), pregnant women with physiological pregnancy (24-28 weeks) without any signs of periodontal disease (10), pregnant with a generalized periodontitis I--II degrees in remission (10), women with pathological pregnancy with no signs of periodontal inflammation (10). In each of the groups over two samples of saliva were collected, the first collection of saliva in the morning on an empty stomach. Then mouthwash 0.9% sodium chloride solution was assigned and after 30 minutes the second portion of saliva. By enzyme immunoassay in samples of saliva of control groups of nonpregnant and pregnant women, as well as women with signs of a pathological course of pregnancy, the content of estriol, testosterone, and dehydroepiandrosterone sulfate was determined.
  
  
  METHODS
  Statistical data analysis was performed by the standard technique using Student's t-test.
  
  
  RESULTS
  The results of biochemical analysis of saliva samples collected before rinsing the mouth with saline in groups of healthy nonpregnant and pregnant women were compared. It was established that during pregnancy the concentration of salivary estriol increases, but in pregnant women with periodontitis, the amount of this hormone in the saliva was significantly reduced. The highest content of testosterone in saliva samples, observed in healthy pregnant women, was significantly higher than nonpregnant women. In pregnant women with periodontitis concentration of testosterone in saliva is reduced, while remaining significantly higher than its level in the saliva of nonpregnant women. The highest concentration of testosterone is observed in the saliva of healthy pregnant women with periodontitis, but the smallest concentration of testosterone is found in the saliva of nonpregnant women. Also the nonpregnant group has the lowest levels of DHEA in pregnancy, and its content increases almost threefold when periodontal disease further grows.
  
  
  CONCLUSIONS
  It was established that periodontitis against pregnancy is characterized by higher levels of salivary DHEA sulfate and lower estriol, compared with a control group of pregnant women.
authors.affiliation: Department of Biology, Odessa State Environmental University, Odessa, Ukraine.
authors.forename: S I
authors.initials: SI
authors.lastname: Dolomatov
authors.forename: W
authors.initials: W
authors.lastname: Zukow
authors.forename: I D
authors.initials: ID
authors.lastname: Atmazhov
authors.forename: R
authors.initials: R
authors.lastname: Muszkieta
authors.forename: A
authors.initials: A
authors.lastname: Skaliy
keywords: Man; parodontitis; pregnancy; saliva