title: Effect of interferon-γ on NF-κB and cytokine IL-18 and IL-27 in acute pancreatitis.
abstract: The objective of our work was to evaluate the effect of interferon-γ (IFN-γ) on cytokine expression in rat acute pancreatitis (AP). AP was introduced to rats which were divided into Control, AP and IFN-γ group. Rats in the AP and IFN-γ group were sacrificed as 6, 12 and 24 h after IFN-γ treatment. The serum amylase (AMA), endotoxin and cytokines were detected. The pathological examination and immunofluorescence staining of pancreas for TNF-α, NF-κB and IL-18 were performed. The serum AMA increased significantly at 6 h and reduced at 48 h after AP. The increase in IFN-γ was higher than that in AMA. IL-18 increased in the AP and IFN group, and IFN increased markedly at 48 h after AP. IL-27 reduced at 24 h after AP compared with AP group. In the AP group, the immunostaining of cytokines increased. In the IFN group, the edema in the pancreas was more severe, and NF-κB and IL-18 expression was higher than that in the other two groups. IFN-γ can increase serum IL-18 and reduce IL-27 in AP. IFN-γ can increase serum IL-18 and reduce serum IL-27 in AP. The increase in NF-κB and IL-18 may exert influence on pro-inflammatory cytokines to deteriorate inflammation in the pancreas. Thus, to control the IFN-γ might has promise to attenuate pancreatitis.
authors.affiliation: Department of General Surgery, Tenth People's Hospital, Tongji University School of Medicine, 301 Middle Yanchang Road, Shanghai 200072, China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Meng
authors.forename: Jian
authors.initials: J
authors.lastname: Gong
authors.forename: Lin
authors.initials: L
authors.lastname: Fang
authors.forename: Zhenshun
authors.initials: Z
authors.lastname: Song
authors.forename: Fan
authors.initials: F
authors.lastname: Wu
authors.forename: Bo
authors.initials: B
authors.lastname: Zhou
authors.forename: Mingping
authors.initials: M
authors.lastname: Qian
mesh_terms: D000681:Amylases / Q000097:blood; D000818:Animals; D016207:Cytokines / Q000378:metabolism; D004731:Endotoxins / Q000378:metabolism; D007249:Inflammation / Q000378:metabolism; D007371:Interferon-gamma / Q000494:pharmacology*; D020382:Interleukin-18 / Q000378:metabolism*; D007378:Interleukins / Q000378:metabolism*; D008297:Male; D008856:Microscopy, Fluorescence; D016328:NF-kappa B / Q000378:metabolism*; D010195:Pancreatitis / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D016207:Cytokines; D004731:Endotoxins; D020382:Interleukin-18; D007378:Interleukins; D016328:NF-kappa B; C556273:interleukin-27, rat; D007371:Interferon-gamma; D000681:Amylases