title: Genetic structure of the endangered northeastern bulrush (Scirpus ancistrochaetus) in Pennsylvania, USA, using information from RAPD and SNPs.
abstract: The northeastern bulrush, Scirpus ancistrochaetus, is a federally endangered wetland plant species found primarily in Pennsylvania, USA. Data on the population genetic structure of this species are needed by conservation managers to prioritize conservation efforts. In this study, we used two genetic marker systems to examine diversity and structure of this species in populations throughout Pennsylvania. The first simple and inexpensive approach utilized RAPD primers; our second, more detailed approach relied on DNA sequencing of single nucleotide polymorphisms. We found genetic variation using both RAPDs and sequencing and found some overlap in information between the two methods, including clusters of related populations and the identification of a genetically unique population. Future studies will seek to examine variation across the full geographic range of the species.
authors.affiliation: Wilmington College, 1870 Quaker Way, Wilmington, OH, 45177, USA, KAL143@alumni.psu.edu.
authors.forename: Kendra
authors.initials: K
authors.lastname: Cipollini
authors.forename: Kendra C
authors.initials: KC
authors.lastname: Millam
authors.forename: Douglas
authors.initials: D
authors.lastname: Burks
authors.forename: Don
authors.initials: D
authors.lastname: Cipollini
authors.forename: Sarah
authors.initials: S
authors.lastname: Girod
authors.forename: Zachary
authors.initials: Z
authors.lastname: VanGundy
authors.forename: Jeffrey L
authors.initials: JL
authors.lastname: Peters
mesh_terms: D003247:Conservation of Natural Resources; D029785:Cyperaceae / Q000145:classification / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D056727:Endangered Species; D005819:Genetic Markers; D014644:Genetic Variation; D010414:Pennsylvania; D020641:Polymorphism, Single Nucleotide; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers