title: A 3-D airway epithelial cell and macrophage co-culture system to study Rhodococcus equi infection.
abstract: We developed a 3-D equine bronchial epithelial cell (BEC) culture that fully differentiates into ciliary beating and mucus producing cells. Using this system, we evaluated how mucus affects the phagocytic activity of macrophages. Adult horse monocyte-derived macrophages were incubated with Rhodococcus equi for 4h either in the mucus layer of in vitro generated airway epithelium or on collagen coated membranes. Using light and electron microscopy, we noted that the number of macrophages with intracellular bacteria, and the number of intracellular bacteria per macrophage were lower in the presence of mucus. TNFα measurements revealed that the presence of BECs promoted TNFα production by R. equi-infected macrophages; a decrease in TLR-2 (involved in R. equi recognition) and an increase in EGF-R (involved in mucin production) mRNA expression were also noted. Interestingly, when foal macrophages were added to foal BECs, we made the opposite observation, i.e. many macrophages were loaded with R. equi. Our in vitro bronchial system shows great potential for the identification of mechanisms how BECs and mucus play a role in phagocyte activation and bacterial clearance. Further studies using this system will show whether the airway environment in the foal responds differently to R. equi infection.
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA. ues3@cornell.edu
authors.forename: Ute
authors.initials: U
authors.lastname: Schwab
authors.forename: Shannon
authors.initials: S
authors.lastname: Caldwell
authors.forename: Mary-Beth
authors.initials: MB
authors.lastname: Matychak
authors.forename: Julia
authors.initials: J
authors.lastname: Felippe
mesh_terms: D000818:Animals; D018920:Coculture Techniques / Q000662:veterinary; D004847:Epithelial Cells / Q000166:cytology / Q000382:microbiology / Q000502:physiology*; D008264:Macrophages / Q000166:cytology / Q000382:microbiology / Q000502:physiology*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016929:Rhodococcus equi; D014409:Tumor Necrosis Factor-alpha
chemical_list: D014409:Tumor Necrosis Factor-alpha