title: Hyposialylated α1-acid glycoprotein inhibits phagocytosis of feline neutrophils.
abstract: Feline α1-acid glycoprotein (fAGP) modifies both its serum concentration and its glycan moiety during diseases. fAGP is hyposialylated in cats with feline infectious peritonitis (FIP), but not in clinically healthy cats or in cats with other diseases. This study was aimed to determine whether hyposialylated fAGP influences phagocytosis. A flow cytometric method based on ingestion of fluoresceinated bacteria and adapted to feline blood was used to assess phagocytosis of leukocytes incubated with 'non-pathological' fAGP (purified from sera with normal concentrations of AGP) and 'pathological' fAGP (purified from sera with >1.5mg/mL hyposialylated AGP). The flow cytometric method provided repeatable results for neutrophils (coefficients of variations, CVs <15%) but not for monocytes (CVs>20%) which had also a high individual variability. Compared with saline solution and with non-pathological fAGP, pathological fAGP significantly decreased phagocytosis in neutrophils and monocytes. This study demonstrated that hyposialylated fAGP down-regulates the phagocytic activity of feline neutrophils.
authors.affiliation: Department of Veterinary Sciences and Public Health, University of Milan, Via Celoria 10, 20133 Milan, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Rossi
authors.forename: L
authors.initials: L
authors.lastname: Capitani
authors.forename: F
authors.initials: F
authors.lastname: Ceciliani
authors.forename: L
authors.initials: L
authors.lastname: Restelli
authors.forename: S
authors.initials: S
authors.lastname: Paltrinieri
mesh_terms: D000818:Animals; D002236:Carbohydrate Conformation; D002415:Cats; D016766:Feline Infectious Peritonitis / Q000378:metabolism*; D007962:Leukocytes / Q000187:drug effects; D009504:Neutrophils / Q000187:drug effects* / Q000502:physiology*; D009961:Orosomucoid / Q000378:metabolism*; D010587:Phagocytosis / Q000187:drug effects*; D012794:Sialic Acids / Q000378:metabolism
chemical_list: D009961:Orosomucoid; D012794:Sialic Acids
keywords: Feline; Feline infectious peritonitis; Flow cytometry; Phagocytosis; α-1-Acid glycoprotein