title: [Variability of DNA simple sequence repeats in peripheral blood of humans subjected to prolonged exposures of ionizing radiation].
abstract: Long-term post-radiation changes in the level of microsatellite DNA polymorphism in peripheral blood of the male "Mayak" employees (Ozyorsk, Russia), who had been exposed to prolonged gamma-irradiation during professional activities, were studied. DNA samples were obtained from the Radiobiology Repository of Human Tissue (Southern-Urals Biophysics Institute FMBA) and used as templates for arbitrarily primed PCR. Comparative analysis of the obtained samples of DNA fragments showed a significant increase in the number of high-molecular fragments and reduction in the number of amplified low molecular weight DNA fragments in comparison with the control. However, a direct correlation of the level of DNA polymorphism with the accumulated total dose of radiation was not found. The study of the polymorphism of microsatellite DNA repeats can be used for qualitative assessment of the levels of genetic variability.
authors.forename: M G
authors.initials: MG
authors.lastname: Lomaeva
authors.forename: L V
authors.initials: LV
authors.lastname: Malakhova
authors.forename: M L
authors.initials: ML
authors.lastname: Zakharova
authors.forename: S N
authors.initials: SN
authors.lastname: Sokolova
authors.forename: L A
authors.initials: LA
authors.lastname: Fomenko
authors.forename: V N
authors.initials: VN
authors.lastname: Antipova
authors.forename: I Iu
authors.initials: IIu
authors.lastname: Soboleva
authors.forename: V G
authors.initials: VG
authors.lastname: Bezlepkin
authors.forename: E N
authors.initials: EN
authors.lastname: Kirillova
authors.forename: A I
authors.initials: AI
authors.lastname: Gaziev
mesh_terms: D004247:DNA / Q000097:blood / Q000235:genetics / Q000528:radiation effects; D005720:Gamma Rays; D014644:Genetic Variation / Q000528:radiation effects*; D006801:Humans; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D016273:Occupational Exposure; D016133:Polymerase Chain Reaction; D018788:Radioactive Hazard Release; D012426:Russia
chemical_list: D004247:DNA