title: A role for the Ankyrin repeat containing protein Ankrd17 in Nod1- and Nod2-mediated inflammatory responses.
abstract: Innate immune responses induced by the pattern-recognition receptors Nod1 and Nod2 play pivotal roles to combat infection and to instruct adaptive immunity. Here we identify Ankrd17 as a novel binding partner of Nod2 and show that its N-terminal domain mediates Nod2 binding. Knock-down and overexpression analysis revealed that Ankrd17 is functionally involved in Nod2- and Nod1-mediated responses in human myeloid and epithelial cells. In HeLa cells Ankrd17 contributed to pro-inflammatory responses induced by Shigella flexneri, however not to type I interferon responses induced by Sendai virus. In conclusion, this reveals a novel function for Ankrd17 in anti-bacterial innate immune pathways.
authors.affiliation: Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Germany.
authors.forename: Maureen
authors.initials: M
authors.lastname: Menning
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Kufer
mesh_terms: D017089:Ankyrin Repeat; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000502:physiology*; D055785:Gene Knockdown Techniques; D057809:HEK293 Cells; D006367:HeLa Cells; D054884:Host-Pathogen Interactions; D006801:Humans; D007113:Immunity, Innate; D018836:Inflammation Mediators / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism; D053474:Nod1 Signaling Adaptor Protein / Q000378:metabolism*; D053473:Nod2 Signaling Adaptor Protein / Q000378:metabolism*; D011485:Protein Binding; D034741:RNA, Small Interfering / Q000235:genetics; D016601:RNA-Binding Proteins; D029082:Sendai virus / Q000276:immunology; D012763:Shigella flexneri / Q000276:immunology; D051195:Toll-Like Receptor 2 / Q000378:metabolism
chemical_list: C481273:ANKRD17 protein, human; D004268:DNA-Binding Proteins; C508600:IL6 protein, human; D018836:Inflammation Mediators; D015850:Interleukin-6; C119067:NOD1 protein, human; C429018:NOD2 protein, human; D053474:Nod1 Signaling Adaptor Protein; D053473:Nod2 Signaling Adaptor Protein; D034741:RNA, Small Interfering; D016601:RNA-Binding Proteins; C495342:TLR2 protein, human; D051195:Toll-Like Receptor 2