title: Exogenous osmolytes suppresses the toxic effects of malathion on Anabaena variabilis.
abstract: Role of osmolytes is though well established for salt, drought and chilling stress, but their role in pesticide stress is yet to be explored thoroughly. The sporadic information covers our previous studies on proline with respect to endosulfan and carbaryl pesticides in cyanobacteria. Therefore, during the present investigation importance of osmolytes (exogenous and endogenous) is studied in cyanobacterial biofertilizer Anabaena variabilis in the presence of 25, 50, 75 and 100 μg mL(-1) malathion pesticide. Present investigation has two parts. In the first part we showed that malathion exert its toxic effect on growth (biomass) via. malondialdehyde (MDA) and hydrogen peroxide (H2O2). This was associated with quantitative enhancement of endogenous osmolytes (proline, sucrose, mannitol, trehalose and glycogen). In the second part effort was made to corelate effect of exogenous addition of osmolytes (which were detected in the first part of this study) on growth and antioxidant enzymes [like superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX)] of A. variabilis in the presence of 100 μg mL(-1) malathion. Surprisingly it was observed that exogenous osmolytes gave additional protection to the organism. The order of protection provided by osmolytes was as trehalose>glycogen>sucrose>mannitol>proline in A. variabilis.
authors.affiliation: Cyanobacteria Biotechnology Laboratory, Department of Biosciences, Jamia Millia Islamia (Central University), New Delhi 110025, India.
authors.forename: Manikar
authors.initials: M
authors.lastname: Ningthoujam
authors.forename: Khalid
authors.initials: K
authors.lastname: Habib
authors.forename: Fareha
authors.initials: F
authors.lastname: Bano
authors.forename: Sunaina
authors.initials: S
authors.lastname: Zutshi
authors.forename: Tasneem
authors.initials: T
authors.lastname: Fatma
mesh_terms: D046870:Anabaena variabilis / Q000187:drug effects / Q000502:physiology*; D060387:Ascorbate Peroxidases / Q000378:metabolism; D002374:Catalase / Q000494:pharmacology; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000633:toxicity; D006003:Glycogen / Q000737:chemistry; D006861:Hydrogen Peroxide / Q000378:metabolism; D007306:Insecticides / Q000737:chemistry / Q000633:toxicity*; D008294:Malathion / Q000737:chemistry / Q000633:toxicity*; D008315:Malondialdehyde / Q000378:metabolism; D008353:Mannitol / Q000737:chemistry; D011392:Proline / Q000737:chemistry; D013395:Sucrose / Q000737:chemistry; D013482:Superoxide Dismutase / Q000378:metabolism; D014199:Trehalose / Q000737:chemistry
chemical_list: D002800:Cholinesterase Inhibitors; D007306:Insecticides; D008353:Mannitol; D008315:Malondialdehyde; D013395:Sucrose; D006003:Glycogen; D011392:Proline; D014199:Trehalose; D006861:Hydrogen Peroxide; D060387:Ascorbate Peroxidases; D002374:Catalase; D013482:Superoxide Dismutase; D008294:Malathion