title: A duplex PCR for rapid and simultaneous detection of Brucella spp. in human blood samples.
abstract: OBJECTIVE
  To design a duplex PCR for rapid and simultaneous detection of Brucella species. in human blood samples.
  
  
  METHODS
  Fifty-two peripheral bloods samples were collected from suspicious patients with brucellosis. Following DNA extraction, PCR assay were performed, using three primers that could simultaneously identify and differentiate three major species of pathogenic Brucella in humans and animals.
  
  
  RESULTS
  Of the 52 peripheral bloods samples tested, 25 sample (48%) showed positive reactions in PCR. Twelve samples were positive for Brucella abortus 39 (B. abortus 39) (23%), 13 for Brucella melitensis 39 (B. melitensis 39) (25%) and 0 for Brucella ovis 39 (B. ovis 39) (0%).
  
  
  CONCLUSIONS
  This work demonstrates that in case where specific primers were utilized, duplex PCR has proved to be a simple, fast, and relatively inexpensive method for simultaneous detection of important species of Brucella in clinical samples.
authors.affiliation: Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Mirnejad
authors.forename: Mozafar
authors.initials: M
authors.lastname: Mohamadi
authors.forename: Vahbeh
authors.initials: V
authors.lastname: Piranfar
authors.forename: Seied Mojtaba
authors.initials: SM
authors.lastname: Mortazavi
authors.forename: Reza
authors.initials: R
authors.lastname: Kachuei
mesh_terms: D000328:Adult; D016470:Bacteremia / Q000175:diagnosis / Q000382:microbiology*; D002002:Brucella / Q000235:genetics / Q000302:isolation & purification*; D002006:Brucellosis / Q000097:blood / Q000175:diagnosis / Q000382:microbiology*; D004269:DNA, Bacterial / Q000097:blood; D004587:Electrophoresis, Agar Gel; D005260:Female; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004269:DNA, Bacterial