title: Associations between two single nucleotide polymorphisms of the adiponectin gene, its circulating concentrations and cardiometabolic risk factors in prepubertal children with and without abdominal obesity.
abstract: BACKGROUND
  The adiponectin gene has been identified as a susceptibility locus for metabolic syndrome, diabetes and cardiovascular disease.
  
  
  OBJECTIVE
  To examine the influence of two single nucleotide polymorphisms (SNPs) of this gene (+276G>T and +45T>G) on circulating adiponectin concentrations, and to evaluate their relationship with adiposity and cardiometabolic risk factors in prepubertal children with and without abdominal obesity.
  
  
  METHODS
  168 children (78M, 6-10 yr) were examined, divided into three groups based on waist circumference (WC). Auxological and biochemical parameters were measured by standard procedures. Adiponectin SNPs were genotyped using TaqMan allelic discrimination assays.
  
  
  RESULTS
  Adiponectin concentration correlated inversely with measures of adiposity (rBMIz-score=-0.211, pBMIz-score=0.007; rwc=-0.210, pwc=0.008; rwc/height=-0.215, pwc/height=0.006), and was significantly influenced by blood glucose, insulin and systolic blood pressure (SBP). The +276T-allele carriers had higher SBP and diastolic BP compared to GG-homozygotes (p<0.05), and expressed higher obesity-related measures and lower adiponectin concentrations. As to the +45T>G SNP, the GGsubject had higher total cholesterol and LDL-C concentrations compared to the T-allele carriers (p<0.05), showing worse obesity measures, higher triglyceride, glucose and insulin and lower serum adiponectin values.
  
  
  CONCLUSIONS
  Genetic variants of the adiponectin gene had an impact on adiposity, adiponectin concentrations and some cardiometabolic variables among prepubertal children.
authors.affiliation: Department of Paediatrics and Medical Genetics, Varna Medical University, 55, Marin Drinov str., Varna 9002, Bulgaria. sonya_galcheva@mail.bg.
authors.forename: S V
authors.initials: SV
authors.lastname: Galcheva
authors.forename: D
authors.initials: D
authors.lastname: Martorana
authors.forename: V M
authors.initials: VM
authors.lastname: Iotova
authors.forename: Y
authors.initials: Y
authors.lastname: Yotov
authors.forename: T M
authors.initials: TM
authors.lastname: Neri
authors.forename: M E
authors.initials: ME
authors.lastname: Street
mesh_terms: D052242:Adiponectin / Q000097:blood* / Q000235:genetics*; D015415:Biomarkers / Q000032:analysis; D002318:Cardiovascular Diseases / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D016022:Case-Control Studies; D002648:Child; D003430:Cross-Sectional Studies; D005260:Female; D005500:Follow-Up Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007333:Insulin Resistance; D008297:Male; D024821:Metabolic Syndrome / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D056128:Obesity, Abdominal / Q000150:complications* / Q000235:genetics / Q000473:pathology; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011379:Prognosis; D011627:Puberty / Q000235:genetics*; D012307:Risk Factors; D055105:Waist Circumference
chemical_list: D052242:Adiponectin; D015415:Biomarkers