title: Cyclic di-AMP is critical for Listeria monocytogenes growth, cell wall homeostasis, and establishment of infection.
abstract: Listeria monocytogenes infection leads to robust induction of an innate immune signaling pathway referred to as the cytosolic surveillance pathway (CSP), characterized by expression of beta interferon (IFN-β) and coregulated genes. We previously identified the IFN-β stimulatory ligand as secreted cyclic di-AMP. Synthesis of c-di-AMP in L. monocytogenes is catalyzed by the diadenylate cyclase DacA, and multidrug resistance transporters are necessary for secretion. To identify additional bacterial factors involved in L. monocytogenes detection by the CSP, we performed a forward genetic screen for mutants that induced altered levels of IFN-β. One mutant that stimulated elevated levels of IFN-β harbored a transposon insertion in the gene lmo0052. Lmo0052, renamed here PdeA, has homology to a cyclic di-AMP phosphodiesterase, GdpP (formerly YybT), of Bacillus subtilis and is able to degrade c-di-AMP to the linear dinucleotide pApA. Reduction of c-di-AMP levels by conditional depletion of the di-adenylate cyclase DacA or overexpression of PdeA led to marked decreases in growth rates, both in vitro and in macrophages. Additionally, mutants with altered levels of c-di-AMP had different susceptibilities to peptidoglycan-targeting antibiotics, suggesting that the molecule may be involved in regulating cell wall homeostasis. During intracellular infection, increases in c-di-AMP production led to hyperactivation of the CSP. Conditional depletion of dacA also led to increased IFN-β expression and a concomitant increase in host cell pyroptosis, a result of increased bacteriolysis and subsequent bacterial DNA release. These data suggest that c-di-AMP coordinates bacterial growth, cell wall stability, and responses to stress and plays a crucial role in the establishment of bacterial infection.
authors.affiliation: Graduate Group in Microbiology, University of California, Berkeley, Berkeley, California, USA.
authors.forename: Chelsea E
authors.initials: CE
authors.lastname: Witte
authors.forename: Aaron T
authors.initials: AT
authors.lastname: Whiteley
authors.forename: Thomas P
authors.initials: TP
authors.lastname: Burke
authors.forename: John-Demian
authors.initials: JD
authors.lastname: Sauer
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Portnoy
authors.forename: Joshua J
authors.initials: JJ
authors.lastname: Woodward
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D002473:Cell Wall / Q000502:physiology*; D004251:DNA Transposable Elements; D015226:Dinucleoside Phosphates / Q000378:metabolism*; D005260:Female; D015964:Gene Expression Regulation, Bacterial; D055786:Gene Knockout Techniques; D006706:Homeostasis; D008089:Listeria monocytogenes / Q000201:enzymology / Q000254:growth & development* / Q000378:metabolism / Q000472:pathogenicity*; D008088:Listeriosis / Q000382:microbiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D016254:Mutagenesis, Insertional; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D015226:Dinucleoside Phosphates; D037521:Virulence Factors; C528998:cyclic diadenosine phosphate