title: Prior reproductive experience alters prolactin-induced macrophage responses in pregnant rats.
abstract: Reproductive experience (i.e., pregnancy and lactation) induces physiological changes in mammals. A previous reproductive experience was recently shown to modulate the activity of dopaminergic hypothalamic systems while decreasing serum prolactin levels and oxidative burst activity in peritoneal macrophages. Dopamine receptor antagonists increase serum prolactin levels, and both prolactin and dopamine receptors may be involved in the modulation of macrophage activity, providing a means of communication between the nervous and immune systems. The present study evaluated the in vitro effects of prolactin and a dopamine D2 receptor antagonist on the peritoneal activity of macrophages from primigravid and multigravid female rats during the third trimester of pregnancy. Oxidative bursts and phagocytosis in peritoneal macrophages were evaluated by flow cytometry. Primigravid and multigravid Wistar rats, during the third trimester of pregnancy (i.e., days 17-21), were used. Peritoneal fluid samples from these rats were first incubated with prolactin (10 and 100 nM) for different periods of time. The same procedure was repeated to evaluate the effects of domperidone (10 and 100 nM) on macrophage activity. Our results showed that macrophages from multigravid rats responded more effectively to in vitro incubation with prolactin, especially with regard to the intensity and percentage of phagocytosis. Additionally, these effects were more pronounced after incubation periods of 30 min or 4 h. These data suggest that macrophages during a second pregnancy become more sensitive to the phagocytotic effects of prolactin.
authors.affiliation: Department of Pathology, School of Veterinary Medicine, University of São Paulo, São Paulo, SP, Brazil. Electronic address: rothvet@gmail.com.
authors.forename: Maria Isabel Roth
authors.initials: MI
authors.lastname: Carvalho-Freitas
authors.forename: Janete A
authors.initials: JA
authors.lastname: Anselmo-Franci
authors.forename: João
authors.initials: J
authors.lastname: Palermo-Neto
authors.forename: Luciano F
authors.initials: LF
authors.lastname: Felicio
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004294:Domperidone / Q000494:pharmacology; D065127:Dopamine D2 Receptor Antagonists; D005260:Female; D020073:Gravidity / Q000276:immunology*; D008262:Macrophage Activation / Q000187:drug effects; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects / Q000276:immunology; D010587:Phagocytosis / Q000187:drug effects / Q000276:immunology; D011247:Pregnancy / Q000276:immunology; D011264:Pregnancy Trimesters; D011388:Prolactin / Q000097:blood / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012098:Reproduction / Q000276:immunology
chemical_list: D065127:Dopamine D2 Receptor Antagonists; D004294:Domperidone; D011388:Prolactin
keywords: Domperidone; Innate immunity; Neuroimmunomodulation; Pregnancy