title: [Preliminary study of the dual release baicalin and rhBMP-2 system to improve periodontal tissue regeneration in minipigs].
abstract: OBJECTIVE
  To explore the feasibility of dual release baicalin and rhBMP-2 system for the treatment of periodontal diseases in minipigs.
  
  
  METHODS
  Four miniature swines were selected to establish the model of periodontitis and randomly divided them into four groups: Dual release group, hydrogel with baicalin-GMS and rhBMP-2; Single Huang group, hydrogel with baicalin-GMS; BMP group, hydrogel with rhBMP-2; Negative control group, blank hydrogel. All parameters including clinical indicators of periodontitis, the level of IL-1 and TNF-α in the gingival crevicular fluid (GCF) were measured before and 6 weeks after modeling, and 4, 8 weeks after treatment. The data was analyzed using SPSS13.0 software package. The periodontitis model animals were sacrificed at the end of 8 weeks after treatment. The histological changes of periodontal tissues were observed under microscope with HE staining.
  
  
  RESULTS
  (1)In all groups, there were significant differences of clinical indicators and levels of IL-1 and TNF-α in the GCF between periodontitis was established and 4, 8 weeks after treatment (P<0.05). Dual release group was significantly superior than that of other three groups (P<0.05). Moreover, all the parameters improved continuously. X-ray showed that bone mineral density and height in the remaining three groups increased compared with the control group, with highest in dual release group. (2)The results of histological observation showed that the inflammation reaction of periodontal tissues in negative control group was more serious than that in other groups 8 weeks after treatment. There was no obvious repair reaction in the control group, but different amount of new bone was seen in other three groups. Among them, dual release group had most obvious repair reaction.
  
  
  CONCLUSIONS
  The dual-slow-release chitosan thermosensitive hydrogel system is employable to be used effectively in the regeneration of periodontal defects, which laid a foundation for further clinical use.
authors.affiliation: Department of Periodontology, Xi'an Jiaotong University. Xi'an, Shanxi Province, China. zenghui310@yahoo.com.cn
authors.forename: Hui
authors.initials: H
authors.lastname: Zeng
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Wei
authors.forename: Jian-feng
authors.initials: JF
authors.lastname: Shi
authors.forename: Guo-zhou
authors.initials: GZ
authors.lastname: Rao
authors.forename: Ang
authors.initials: A
authors.lastname: Li
authors.forename: Jian-zhong
authors.initials: JZ
authors.lastname: Gou
mesh_terms: D000818:Animals; D005419:Flavonoids; D005883:Gingival Crevicular Fluid; D010510:Periodontal Diseases; D010518:Periodontitis; D010519:Periodontium; D012038:Regeneration; D013552:Swine; D013556:Swine, Miniature; D014409:Tumor Necrosis Factor-alpha; D014945:Wound Healing
chemical_list: D005419:Flavonoids; D014409:Tumor Necrosis Factor-alpha; C038044:baicalin