title: Binding and inhibition of protease enzymes, including MMPs, by a superabsorbent dressing in vitro.
abstract: OBJECTIVE
  To demonstrate the binding and inactivation action of a superabsorbent dressing on proteolytic enzymes MMP-2, MMP-9 and collagenase using an established methodology.
  
  
  METHODS
  An in vitro assay of MMP binding and collagenase inactivation has been conducted using the superabsorbent wound dressing (Eclypse; Advancis Medical UK). Dressings in this category, and other absorbents, have been claimed to possess MMP-binding characteristics; however, for most there is no published evidence as yet. In this series of experiments, we have used validated experimental techniques to evaluate such activity.
  
  
  RESULTS
  Results show that the superabsorbent dressing does have a statistically-significant effect in binding two of the most important MMPs, MMP-2 and MMP-9, as well as inhibiting collagenase.
  
  
  CONCLUSIONS
  These results support this activity for the superabsorbent dressing and indicate a probable beneficial clinical action in reducing the influence of these enzymes in delayed wound healing.
authors.affiliation: Department of Dermatology, University Medical Centre Jena, Germany. c.wiegand@med.uni-jena.de
authors.forename: C
authors.initials: C
authors.lastname: Wiegand
authors.forename: R J
authors.initials: RJ
authors.lastname: White
mesh_terms: D000042:Absorption; D001458:Bandages; D002478:Cells, Cultured; D017364:Collagenases / Q000378:metabolism*; D005122:Exudates and Transudates / Q000201:enzymology*; D006801:Humans; D008422:Materials Testing; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D011485:Protein Binding; D014947:Wounds and Injuries / Q000201:enzymology / Q000628:therapy*
chemical_list: D017364:Collagenases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9