title: Parapedobacter pyrenivorans sp. nov., isolated from a pyrene-degrading microbial enrichment, and emended description of the genus Parapedobacter.
abstract: A novel pyrene-degrading, Gram-negative bacterium, designated strain P-4(T), was isolated from a polycyclic aromatic hydrocarbon-degrading enrichment of polluted soils from a coking chemical plant. Cells of strain P-4(T) were non-motile rods. Strain P-4(T) grew at 15-45 °C (optimum, 37 °C), pH 6.0-10.0 (optimum, pH 8.5) and 0-4 % (w/v) NaCl. Analysis of the 16S rRNA gene sequence showed that strain P-4(T) was related phylogenetically to members of the genus Parapedobacter, with sequence similarity of 93.7-95.1 %. The cellular fatty acids of strain P-4(T) were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C17 : 0 3-OH, summed feature 9 (iso-C17 : 1ω9c and/or 10-methyl C16 : 0), anteiso-C15 : 0, iso-C15 : 0 3-OH, C16 : 0, iso-C15 : 1 G, C16 : 0 3-OH and C17 : 0 2-OH. Cells contained menaquinone 7 as the major quinone. The polyamine of strain P-4(T) was homospermidine, and the main polar lipids were phosphatidylethanolamine and a sphingolipid. The G+C content of the DNA was 45.4 mol%. Strain P-4(T) showed a range of phenotypic characteristics that differentiated it from previously recognized Parapedobacter species, particularly its ability to use pyrene as a sole carbon source for growth and its alkaline optimal pH for growth (pH 8.5). On the basis of these results, it is concluded that strain P-4(T) represents a novel species of the genus Parapedobacter, for which the name Parapedobacter pyrenivorans (type strain P-4(T) = NBRC 109113(T) = CGMCC 1.12195(T)) is proposed. An emended description of the genus Parapedobacter is also provided.
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Jian-Kang
authors.initials: JK
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Li
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Ming-Jiang
authors.initials: MJ
authors.lastname: Zhang
authors.affiliation: Environmental Protection Research Institute of Light Industry, Beijing 100089, PR China.
authors.forename: Jing-Hua
authors.initials: JH
authors.lastname: Jin
authors.affiliation: Environmental Microbiology Research Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.|State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Cheng-Ying
authors.initials: CY
authors.lastname: Jiang
authors.affiliation: Environmental Microbiology Research Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.|State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Shuang-Jiang
authors.initials: SJ
authors.lastname: Liu
mesh_terms: D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D002681:China; D003077:Coke; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D011073:Polyamines / Q000737:chemistry; D011721:Pyrenes / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D003077:Coke; D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D011073:Polyamines; D011721:Pyrenes; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C062629:menaquinone 7