title: Bifunctional unnatural sialic acids for dual metabolic labeling of cell-surface sialylated glycans.
abstract: Sialic acid analogues containing a unique chemical functionality or chemical reporter have been metabolically incorporated into sialylated glycans. This process, termed metabolic glycan labeling, has emerged as a powerful tool for studying sialylation as well as other types of glycosylation. Currently, this technique can install only a single functionality. Here we describe a strategy for dual labeling of sialylated glycans using a new class of bifunctional sialic acid analogues containing two distinct chemical reporters at the N-acyl and C9 positions. These bifunctional unnatural sialic acids were metabolically incorporated into cellular glycans, where the two chemical reporters exerted their distinct functions. This approach expands the capability of metabolic glycan labeling to probe sialylation and glycan-protein interactions.
authors.affiliation: Shenzhen Graduate School of Peking University, Shenzhen 518055, China.
authors.forename: Lianshun
authors.initials: L
authors.lastname: Feng
authors.forename: Senlian
authors.initials: S
authors.lastname: Hong
authors.forename: Jie
authors.initials: J
authors.lastname: Rong
authors.forename: Qiancheng
authors.initials: Q
authors.lastname: You
authors.forename: Peng
authors.initials: P
authors.lastname: Dai
authors.forename: Rongbing
authors.initials: R
authors.lastname: Huang
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Tan
authors.forename: Weiyao
authors.initials: W
authors.lastname: Hong
authors.forename: Can
authors.initials: C
authors.lastname: Xie
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Xing
authors.initials: X
authors.lastname: Chen
mesh_terms: D002236:Carbohydrate Conformation; D006367:HeLa Cells; D006801:Humans; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*; D012794:Sialic Acids / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism*; D013499:Surface Properties
chemical_list: D011134:Polysaccharides; D012794:Sialic Acids