title: The glycosylated IgII extracellular domain of EMMPRIN is implicated in the induction of MMP-2.
abstract: EMMPRIN is a widely expressed transmembrane glycoprotein that plays important roles in many physiological and pathological processes, such as tumor invasion and metastasis. It stimulates the production of matrix metalloproteinase (MMPs) by tumor-associated fibroblasts. In the present study, our aim was to (a) to investigate if the IgII loop domain of the extracellular domain (ECD) of EMMPRIN contributes to the MMP production by fibroblasts and (b) to evaluate the significance of glycosylation in this process. For this purpose, we expressed the ECD, IgI, or IgII domains of EMMPRIN, in their glycosylated and non-glycosylated forms, in the heterologous expression systems of P. pastoris and E. coli, respectively. Dermal fibroblasts were treated with purified recombinant domains and proteins from cell extracts and supernatants were analyzed by Western blot and zymography assays. Fibroblasts treated with ECD-, IgI-, and IgII-glycosylated domains of EMMPRIN significantly stimulated the gelatinolytic activity of MMP-2, compared to untreated fibroblasts, whereas no significant effect was observed after treatment with the non-glycosylated ECD, IgI, and IgII domains. Western blot analysis from cell extracts and supernatants revealed that only the glycosylated forms were able to stimulate MMP-2 production and secretion, respectively. Quantitative PCR revealed that this effect was not attributed to transcriptional alterations. This study showed that N-glycosylation was a prerequisite for efficient MMP-2 production, with the IgII loop domain contributing significantly to this process. Perturbation of the function of IgII-EMMPRIN loop could have potential therapeutic value in the inhibition of MMP-2-dependent cancer cell invasion and metastasis.
authors.affiliation: Laboratory of Molecular Biology & Immunobiotechnology, Hellenic Pasteur Institute, Athens, Greece.
authors.forename: Adriana
authors.initials: A
authors.lastname: Papadimitropoulou
authors.forename: Avgi
authors.initials: A
authors.lastname: Mamalaki
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D051926:Basigin / Q000737:chemistry / Q000378:metabolism*; D002478:Cells, Cultured; D004790:Enzyme Induction; D004926:Escherichia coli; D005347:Fibroblasts / Q000201:enzymology; D006031:Glycosylation; D006801:Humans; D007136:Immunoglobulins / Q000737:chemistry / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010843:Pichia; D011499:Protein Processing, Post-Translational; D017434:Protein Structure, Tertiary
chemical_list: C497886:BSG protein, human; D007136:Immunoglobulins; D051926:Basigin; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2