title: General protocol for the culture of cells on plasma-coated electrospun scaffolds.
abstract: As opposed to culture on standard tissue-treated plastic, cell culture on three-dimensional scaffolds impedes additional challenges with respect to substrate preparation, cell seeding, culture maintenance, and analysis. We herewith present a general route for the culture of primary cells, differentiated cells, or stem cells on plasma-coated, electrospun scaffolds. We describe a method to prepare and fix the scaffolds in culture wells and discuss a convenient method for cell seeding and subsequent analysis by scanning electron microscopy or immunohistology.
authors.affiliation: Swiss Federal Laboratories of Materials Science and Technology, St. Gallen, Switzerland.
authors.forename: A Géraldine
authors.initials: AG
authors.lastname: Guex
authors.forename: Giuseppino
authors.initials: G
authors.lastname: Fortunato
authors.forename: Dirk
authors.initials: D
authors.lastname: Hegemann
authors.forename: Hendrik T
authors.initials: HT
authors.lastname: Tevaearai
authors.forename: Marie-Noëlle
authors.initials: MN
authors.lastname: Giraud
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D051379:Mice; D057139:Nanofibers / Q000737:chemistry / Q000648:ultrastructure; D010949:Plasma / Q000737:chemistry; D054457:Tissue Scaffolds / Q000737:chemistry