title: Inhibition of pathogenic bacterial biofilm by biosurfactant produced by Lysinibacillus fusiformis S9.
abstract: A biosurfactant producing microbe isolated from a river bank was identified as Lysinibacillus fusiformis S9. It was identified with help of biochemical tests and 16S rRNA gene phylogenetic analysis. The biosurfactant S9BS produced was purified and characterized as glycolipid. The biosurfactant showed remarkable inhibition of biofilm formation by pathogenic bacteria like Escherichia coli and Streptococcus mutans. It was interesting to note that at concentration of 40 μg ml(-1) the biosurfactant did not show any bactericidal activity but restricted the biofilm formation completely. L. fusiformis is reported for the first time to produce a glycolipid type of biosurfactant capable of inhibiting biofilm formation by pathogenic bacteria. The biosurfactant inhibited bacterial attachment and biofilm formation equally well on hydrophilic as well as hydrophobic surfaces like glass and catheter tubing. This property is significant in many biomedical applications where the molecule should help in preventing biofouling of surfaces without being toxic to biotic system.
authors.affiliation: Bioresources Engineering Department, CSIR-Institute of Minerals and Materials Technology, Bhubaneswar, 751013, India.
authors.forename: Arun Kumar
authors.initials: AK
authors.lastname: Pradhan
authors.forename: Nilotpala
authors.initials: N
authors.lastname: Pradhan
authors.forename: Lala Behari
authors.initials: LB
authors.lastname: Sukla
authors.forename: Prasanna Kumar
authors.initials: PK
authors.lastname: Panda
authors.forename: Barda Kanta
authors.initials: BK
authors.lastname: Mishra
mesh_terms: D001406:Bacillaceae / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D018441:Biofilms / Q000187:drug effects*; D003470:Culture Media; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017550:Spectroscopy, Fourier Transform Infrared; D013501:Surface-Active Agents / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D003470:Culture Media; D012336:RNA, Ribosomal, 16S; D013501:Surface-Active Agents