title: Expression, purification, crystallization and preliminary X-ray structure analysis of wild-type and L(M196)H-mutant Rhodobacter sphaeroides reaction centres.
abstract: The electron and proton transport mediated by protein-bound cofactors in photosynthesis have been investigated by various methods in order to determine the energetics, the dynamics and the pathway of this process. In purple bacteria, primary photosynthetic charge separation and the build-up of a proton gradient across the periplasmic membrane are catalyzed by the photosynthetic reaction centre (RC). Here, the purification, crystallization and preliminary X-ray analysis of wild-type and L(M196)H-mutant RCs of Rhodobacter sphaeroides are presented, enabling study of the influence of the protein environment of the primary electron donor on the spectral properties and photochemical activity of the RC.
authors.affiliation: Institute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russian Federation. azat@vega.protres.ru
authors.forename: A G
authors.initials: AG
authors.lastname: Gabdulkhakov
authors.forename: T Y
authors.initials: TY
authors.lastname: Fufina
authors.forename: L G
authors.initials: LG
authors.lastname: Vasilieva
authors.forename: U
authors.initials: U
authors.lastname: Mueller
authors.forename: V A
authors.initials: VA
authors.lastname: Shuvalov
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D015964:Gene Expression Regulation, Bacterial; D009154:Mutation / Q000235:genetics*; D045322:Photosynthetic Reaction Center Complex Proteins / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D012242:Rhodobacter sphaeroides / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D045322:Photosynthetic Reaction Center Complex Proteins
keywords: Rhodobacter sphaeroides; bacteriochlorophyll; photosynthetic reaction centres; primary electron donor; site-directed mutagenesis