title: Foundational concepts of cell fate conversion to the hematopoietic lineage.
abstract: Direct conversion of cellular fate provides a potential approach to generate cells of the hematopoietic lineage without the requisite reversion to a pluripotent state via somatic cell reprogramming. The utilization of this technology has enabled transcription factor-mediated conversion of somatic cell types to primitive and mature hematopoietic cells. Recent studies demonstrate that the direct conversion of somatic cells to the hematopoietic lineage likely requires the use of pioneer transcription factors to establish an accessible chromatin state that is responsive to enforced expression of hematopoietic-specific transcription factors, in combination with appropriate culture conditions that facilitate reprogramming. Developing adaptable, experimental strategies that incorporate these parameters should enable the efficient generation of human hematopoietic cells with translational potential.
authors.affiliation: McMaster Stem Cell and Cancer Research Institute, Michael G. DeGroote School of Medicine, McMaster University, Hamilton, Ontario L8S 4L8, Canada; Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario L8N 3Z5, Canada.
authors.forename: Kyle R
authors.initials: KR
authors.lastname: Salci
authors.forename: Brendan A S
authors.initials: BA
authors.lastname: McIntyre
authors.forename: Mickie
authors.initials: M
authors.lastname: Bhatia
mesh_terms: D002454:Cell Differentiation / Q000235:genetics*; D019070:Cell Lineage; D065150:Cellular Reprogramming; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D014157:Transcription Factors