title: Effects of di(n-butyl) and monobutyl phthalate on steroidogenesis pathways in the murine Leydig tumor cell line MLTC-1.
abstract: Di(n-butyl) phthalate (DBP) and its active metabolite monobutyl phthalate (MBP) have been shown to disrupt reproductive organ growth. The objective of this study was to evaluate the effects of DBP/MBP on steroidogenesis in the murine Leydig tumor cell line MLTC-1 in vitro. MLTC-1 cells were incubated with various concentrations of DBP (100, 1, 0.01, and 0μmol/l in DMSO) and MBP (1000, 10, 0.1, and 0μmol/l in DMSO) for 24h. Testosterone secretion was stimulated at the lowest doses and inhibited at higher treatment doses of DBP and MBP. The mRNA levels of the side-chain cleavage enzyme (P450scc), cytochrome p450c17 (P450c17) and 3β-hydroxy-steroid dehydrogenase (3βHSD) were significantly reduced in the phthalate-exposed groups, whereas, the transcription and translation of insulin-like hormone 3 (INSL3) was affected by DBP and MBP. Alterations of the steroidogenic enzymes and INSL3 in MLTC-1 cells may be involved in the biphasic effects of DBP/MBP on androgen production.
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, Tianjin Medical University, Qixiangtai Road No. 22, Heping District, Tianjin 300070, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, Tianjin Medical University, Qixiangtai Road No. 22, Heping District, Tianjin 300070, China.
authors.forename: Qing-Hong
authors.initials: QH
authors.lastname: Zhou
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, Tianjin Medical University, Qixiangtai Road No. 22, Heping District, Tianjin 300070, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Leng
authors.affiliation: Tianjin Center Hospital of Gynecology and Obstetrics, Nankai-sanma Road No. 156, Nankai District, Tianjin 300100, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, Tianjin Medical University, Qixiangtai Road No. 22, Heping District, Tianjin 300070, China.
authors.forename: Zeng-Rong
authors.initials: ZR
authors.lastname: Sun
authors.affiliation: Department of Occupational and Environmental Health, School of Public Health, Tianjin Medical University, Qixiangtai Road No. 22, Heping District, Tianjin 300070, China. Electronic address: tangnaijun@tijmu.edu.cn.
authors.forename: Nai-Jun
authors.initials: NJ
authors.lastname: Tang
mesh_terms: D015096:3-Hydroxysteroid Dehydrogenases / Q000235:genetics / Q000378:metabolism; D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002786:Cholesterol Side-Chain Cleavage Enzyme / Q000235:genetics / Q000378:metabolism; D003993:Dibutyl Phthalate / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D007328:Insulin / Q000235:genetics / Q000378:metabolism; D007984:Leydig Cell Tumor / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008297:Male; D051379:Mice; D010795:Phthalic Acids / Q000633:toxicity*; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013736:Testicular Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D013739:Testosterone / Q000096:biosynthesis*; D013997:Time Factors
chemical_list: D007328:Insulin; C084797:Leydig insulin-like protein; D010795:Phthalic Acids; D011506:Proteins; D012333:RNA, Messenger; D003993:Dibutyl Phthalate; D013739:Testosterone; D015096:3-Hydroxysteroid Dehydrogenases; D002786:Cholesterol Side-Chain Cleavage Enzyme; C028577:monobutyl phthalate
keywords: 3β-Hydroxysteroid dehydrogenase; Di(n-butyl) phthalate; Insulin-like hormone 3; Monobutyl phthalate; P450 side-chain cleavage enzyme; Testosterone