title: A fatal case of Naegleria fowleri meningoencephalitis in Taiwan.
abstract: After bathing at a hot spring resort, a 75-year-old man presented to the emergency department because of seizure-like attack with loss of conscious. This is the first case of primary amebic meningoencephalitis (PAM) caused by Naegleria fowleri in Taiwan. PAM was diagnosed based on detection of actively motile trophozoites in cerebrospinal fluid using a wet-mount smear and the Liu's stain. The amoebae were further confirmed by PCR and gene sequencing. In spite of administering amphotericin B treatment, the patient died 25 days later.
authors.affiliation: Clinical Laboratory, Chung Shan Medical University Hospital, Taichung, Taiwan.
authors.forename: Mei-Yu
authors.initials: MY
authors.lastname: Su
authors.forename: Ming-Shih
authors.initials: MS
authors.lastname: Lee
authors.forename: Ling-Yuh
authors.initials: LY
authors.lastname: Shyu
authors.forename: Wei-Chen
authors.initials: WC
authors.lastname: Lin
authors.forename: Pei-Ching
authors.initials: PC
authors.lastname: Hsiao
authors.forename: Chi-Ping
authors.initials: CP
authors.lastname: Wang
authors.forename: Dar-Der
authors.initials: DD
authors.lastname: Ji
authors.forename: Ke-Min
authors.initials: KM
authors.lastname: Chen
authors.forename: Shih-Chan
authors.initials: SC
authors.lastname: Lai
mesh_terms: D000368:Aged; D000562:Amebiasis / Q000175:diagnosis* / Q000469:parasitology / Q000473:pathology*; D020808:Central Nervous System Protozoal Infections / Q000175:diagnosis* / Q000469:parasitology / Q000473:pathology*; D002555:Cerebrospinal Fluid / Q000469:parasitology; D016054:DNA, Protozoan / Q000737:chemistry / Q000235:genetics; D017809:Fatal Outcome; D006801:Humans; D008297:Male; D008853:Microscopy; D016848:Naegleria fowleri / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013624:Taiwan
chemical_list: D016054:DNA, Protozoan
keywords: Naegleria fowleri; Taiwan; amphotericin B; fatal case; meningoencephalitis