title: Determination of the attP and attB sites of phage CD27 from Clostridium difficile NCTC 12727.
abstract: The attP region of the Clostridium difficile phage CD27 was identified, located immediately downstream of the putative recombinase. The phage could integrate into two specific sites (attB) in the C. difficile genome, one of which was in an open reading frame encoding a putative ATPase of an ABC transporter and the other in an open reading frame encoding a putative ATPase of the flagella protein export apparatus. The prophage was capable of excision and formation of a circular molecule and phages were spontaneously released at a low frequency during growth. Infection and lysogeny of a C. difficile strain previously shown to be sensitive to CD27 were demonstrated, leading to a reduction in toxin production. Finally, a putative repressor was identified which is likely to be involved in maintaining lysogeny in these strains.
authors.affiliation: Department of Microbial Diseases, UCL Eastman Dental Institute, 256 Gray's Inn Road, London WC1X 8LD, UK.
authors.forename: Rachel
authors.initials: R
authors.lastname: Williams
authors.affiliation: Norwich Medical School, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK.|Institute of Food Research, Norwich Research Park, Colney Lane, Norwich, NR4 7UA, UK.
authors.forename: Emma
authors.initials: E
authors.lastname: Meader
authors.affiliation: Institute of Food Research, Norwich Research Park, Colney Lane, Norwich, NR4 7UA, UK.
authors.forename: Melinda
authors.initials: M
authors.lastname: Mayer
authors.affiliation: Institute of Food Research, Norwich Research Park, Colney Lane, Norwich, NR4 7UA, UK.
authors.forename: Arjan
authors.initials: A
authors.lastname: Narbad
authors.affiliation: Department of Microbial Diseases, UCL Eastman Dental Institute, 256 Gray's Inn Road, London WC1X 8LD, UK.
authors.forename: Adam P
authors.initials: AP
authors.lastname: Roberts
authors.affiliation: Department of Microbial Diseases, UCL Eastman Dental Institute, 256 Gray's Inn Road, London WC1X 8LD, UK.
authors.forename: Peter
authors.initials: P
authors.lastname: Mullany
mesh_terms: D001287:Attachment Sites, Microbiological; D001435:Bacteriophages / Q000235:genetics / Q000502:physiology*; D001483:Base Sequence; D002876:Chromosomes, Bacterial / Q000235:genetics / Q000821:virology; D016360:Clostridioides difficile / Q000821:virology*; D004269:DNA, Bacterial / Q000235:genetics; D004270:DNA, Circular / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D016680:Genome, Bacterial; D016679:Genome, Viral; D008242:Lysogeny; D016366:Open Reading Frames; D039002:Prophages; D014775:Virus Activation; D016662:Virus Integration; D057074:Virus Release
chemical_list: D004269:DNA, Bacterial; D004270:DNA, Circular; D004279:DNA, Viral