title: The SNARE proteins SNAP25 and synaptobrevin are involved in endocytosis at hippocampal synapses.
abstract: SNAP25, an essential component of the soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein receptor (SNARE) complex that mediates exocytosis, is not considered to play a role in endocytosis, which couples to exocytosis by retrieving a similar amount of exocytosed vesicles. By knocking down SNAP25 and imaging slow endocytosis at a conventional synapse, the rat cultured hippocampal synapse, we found that SNAP25 is involved in slow, clathrin-dependent endocytosis. With similar techniques, we found that not only SNAP25, but also synaptobrevin is involved in slow endocytosis. These results provide the first evidence showing the dual role of SNAP25 and synaptobrevin in both exocytosis and slow endocytosis at conventional synapses. Such a dual role may contribute to mediate the coupling between exocytosis and clathrin-dependent endocytosis at conventional synapses, a mechanism critical for the maintenance of synaptic transmission and the normal structure of nerve terminals.
authors.affiliation: National Institute of Neurological Disorders and Stroke, Bethesda, Maryland 20892, USA.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.forename: Jianhua
authors.initials: J
authors.lastname: Xu
authors.forename: Hsueh-Cheng
authors.initials: HC
authors.lastname: Chiang
authors.forename: Wonchul
authors.initials: W
authors.lastname: Shin
authors.forename: Ling-Gang
authors.initials: LG
authors.lastname: Wu
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002966:Clathrin / Q000378:metabolism; D034281:Dynamins / Q000378:metabolism; D004705:Endocytosis / Q000235:genetics / Q000502:physiology*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology*; D066298:In Vitro Techniques; D009474:Neurons / Q000166:cytology*; D016716:PC12 Cells; D050683:R-SNARE Proteins / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D013569:Synapses / Q000378:metabolism*; D050825:Synaptosomal-Associated Protein 25 / Q000378:metabolism*; D014162:Transfection
chemical_list: D002966:Clathrin; D050683:R-SNARE Proteins; D034741:RNA, Small Interfering; C494210:Snap25 protein, rat; D050825:Synaptosomal-Associated Protein 25; D049452:Green Fluorescent Proteins; D034281:Dynamins