title: Highly specific, multi-branched fluorescent reporters for analysis of human neutrophil elastase.
abstract: Human neutrophil elastase (HNE) is a serine protease implicated in the pathogenesis of acute and chronic inflammatory disease. Here a series of, internally quenched, single fluorophore fluorescent reporters were synthesised that allowed the rapid, highly specific and sensitive analysis of HNE activity over closely related proteases.
authors.affiliation: EaStChem, School of Chemistry, University of Edinburgh, West Mains Road, EH9 3JJ, Edinburgh, UK.
authors.forename: Nicolaos
authors.initials: N
authors.lastname: Avlonitis
authors.forename: Manuelle
authors.initials: M
authors.lastname: Debunne
authors.forename: Tashfeen
authors.initials: T
authors.lastname: Aslam
authors.forename: Neil
authors.initials: N
authors.lastname: McDonald
authors.forename: Chris
authors.initials: C
authors.lastname: Haslett
authors.forename: Kevin
authors.initials: K
authors.lastname: Dhaliwal
authors.forename: Mark
authors.initials: M
authors.lastname: Bradley
mesh_terms: D000595:Amino Acid Sequence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D019272:Leukocyte Elastase / Q000032:analysis*; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry*; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D010455:Peptides; D019272:Leukocyte Elastase