title: The role of NADPH oxidase in taurine attenuation of Streptococcus uberis-induced mastitis in rats.
abstract: In order to evaluate the role of taurine on the oxidative stress mediated by NADPH oxidase in Streptococcus uberis-induced (S. uberis) mastitis, rats were administered daily (per os) 100mg/kg of taurine (group TS) or an equal volume of physiological saline (group CS) from gestation day 14 until parturition. Seventy-two hours after parturition, approximately 100cfu of S. uberis was infused into each of 2 mammary glands. Pretreatment with taurine significantly decreased mRNA and protein expression of p47phox and p22phox in mammary tissues. The total anti-oxidation capability (T-AOC) levels and superoxide dismutase (SOD) activities decreased, while malondialdehyde (MDA) levels increased both in mammary tissues and serum of rats with intramammary S. uberis infusion. Gavage administration of taurine moderated this change. Concentrations of interleukin-1β (IL-1β) and IL-6 in mammary glands decreased as a result of taurine administration. Significant differences (P<0.05) were present at 48 and 72 h post S. uberis-infusion (PI) for IL-1β and 72 h PI for IL-6. Our data indicate that, in S. uberis-induced mastitis, taurine has the ability of regulating redox conditions which leads to the suppression of oxidative stress and secretion of proinflammatory cytokines. This phenomenon may be ascribed to taurines's ability to inhibit the expression of NADPH oxidase.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China. mjf171647@126.com
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Miao
authors.forename: Jinqiu
authors.initials: J
authors.lastname: Zhang
authors.forename: Zili
authors.initials: Z
authors.lastname: Ma
authors.forename: Liuhai
authors.initials: L
authors.lastname: Zheng
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D016207:Cytokines / Q000276:immunology; D005260:Female; D008321:Mammary Glands, Animal / Q000187:drug effects / Q000201:enzymology / Q000382:microbiology; D008413:Mastitis / Q000201:enzymology / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D019255:NADPH Oxidases / Q000096:biosynthesis / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D013290:Streptococcal Infections / Q000201:enzymology / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D013291:Streptococcus / Q000472:pathogenicity; D013654:Taurine / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use*; D013997:Time Factors
chemical_list: D000975:Antioxidants; D016207:Cytokines; D013654:Taurine; D019255:NADPH Oxidases; C515768:Cyba protein, rat; C060505:neutrophil cytosolic factor 1