title: Crystallization and preliminary X-ray diffraction analysis of a new xyloglucanase from Xanthomonas campestris pv. campestris.
abstract: Xyloglucanases (Xghs) are important enzymes involved in xyloglucan modification and degradation. Xanthomonas campestris pv. campestris (Xcc) is a phytopathogenic bacterium which produces a large number of glycosyl hydrolases (GH), but has only one family 74 GH (Xcc-Xgh). This enzyme was overexpressed in Escherichia coli, purified and crystallized. Diffraction data sets were collected for the native enzyme and its complex with glucose to maximum resolutions of 2.0 and 2.1 Å, respectively. The data were indexed in a hexagonal crystal system with unit-cell parameters a = b = 153.4, c = 84.9 Å. As indicated by molecular-replacement solution, the crystals belonged to space group P6(1).
authors.affiliation: Instituto de Física de São Carlos, Universidade de São Paulo, 400 Trabalhador Saocarlense, 13566-590 São Carlos, SP, Brazil.
authors.forename: Evandro Ares
authors.initials: EA
authors.lastname: de Araújo
authors.forename: Atílio
authors.initials: A
authors.lastname: Tomazini
authors.forename: Marco Antonio Seiki
authors.initials: MA
authors.lastname: Kadowaki
authors.forename: Mário Tyago
authors.initials: MT
authors.lastname: Murakami
authors.forename: Igor
authors.initials: I
authors.lastname: Polikarpov
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis / Q000737:chemistry*; D003460:Crystallization; D006026:Glycoside Hydrolases / Q000032:analysis / Q000737:chemistry*; D014961:X-Ray Diffraction; D016959:Xanthomonas campestris / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D006026:Glycoside Hydrolases; C072818:xyloglucan endo(1-4)-beta-D-glucanase
keywords: GH74 family; Xanthomonas campestris pv. campestris; xyloglucanase