title: Stable complex formation of thylakoidal processing peptidase and PGRL1.
abstract: The thylakoid-transfer signal is required for energy-dependent translocation of preproteins into the thylakoid lumen and is removed by the thylakoidal processing peptidase (TPP). PGRL1 is an essential component of antimycin A-sensitive photosynthetic cyclic electron flow in chloroplasts. Here we report that one of the TPP isoforms, Plsp1, forms a stable complex with PGRL1. Genetic data demonstrate that PGRL1 is not essential for Plsp1 activity in vivo, leading to a possibility that PGRL1 may act as a regulator of TPP.
authors.affiliation: Department of Plant Sciences, University of California at Davis, One Shields Avenue, Davis, CA 95616, USA.
authors.forename: Joshua K
authors.initials: JK
authors.lastname: Endow
authors.forename: Kentaro
authors.initials: K
authors.lastname: Inoue
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000378:metabolism*; D004795:Enzyme Stability; D005804:Genes, Lethal; D008565:Membrane Proteins / Q000378:metabolism*; D046912:Multiprotein Complexes / Q000378:metabolism; D055503:Protein Multimerization; D036226:Seedlings / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D012697:Serine Endopeptidases / Q000378:metabolism*; D020524:Thylakoids / Q000201:enzymology* / Q000502:physiology
chemical_list: D029681:Arabidopsis Proteins; D008565:Membrane Proteins; D046912:Multiprotein Complexes; C527375:PGRL1 protein, Arabidopsis; D012697:Serine Endopeptidases; C583245:PLSP1 protein, Arabidopsis