title: Detection of transfluthrin and metofluthrin genotoxicity in the ST cross of the Drosophila Wing Spot Test.
abstract: In this study, different concentrations of transfluthrin and metofluthrin have been assayed for genotoxicity by using the Wing Spot Test on Drosophila melanogaster. Standard cross was used in the experiment. Third-instar larvae that were trans-heterozygous for the two genetic markers mwh and flr(3) were treated at different concentrations (0.0103mgmL(-1), 0.103mgmL(-1) for transfluthrin and 6μgmL(-1), 60μgmL(-1) for metofluthrin) of the test compounds. Feeding ended with pupation of the surviving larvae and the genetic changes induced in somatic cells of the wing's imaginal discs lead to the formation of mutant clones on the wing blade. Results indicated that two experimental concentrations of transfluthrin and 60μgmL(-1) metofluthrin showed mutagenic and recombinogenic effects in both the marker-heterozygous (MH) flies and the balancer-heterozygous (BH) flies.
authors.affiliation: Department of Primary School Education, Gazi University, Teknikokullar, 06500 Ankara, Turkey. erabia@gazi.edu.tr
authors.forename: Rabia
authors.initials: R
authors.lastname: Sarıkaya
authors.forename: Burcu Koçak
authors.initials: BK
authors.lastname: Memmi
mesh_terms: D000818:Animals; D003521:Cyclopropanes / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004331:Drosophila melanogaster / Q000166:cytology / Q000187:drug effects* / Q000235:genetics*; D004785:Environmental Pollutants / Q000633:toxicity; D005260:Female; D005464:Fluorobenzenes / Q000633:toxicity*; D006824:Hybridization, Genetic; D008297:Male; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D014921:Wings, Animal / Q000166:cytology / Q000187:drug effects*
chemical_list: D003521:Cyclopropanes; D004785:Environmental Pollutants; D005464:Fluorobenzenes; D009153:Mutagens; C560613:transfluthrin; C492600:(2,3,5,6-tetrafluoro-4-methoxymethylphenyl)methyl-2,2-dimethyl-3-(1-propenyl)cyclopropanecarboxylate
keywords: BH; Balancer-heterozygous flies (mwh +/+ TM3 Bds) with phenotypically serrate wings; Drosophila melanogaster; Frequency of clone formation per 10(5) cells; LOH; Loss of Heterozygosity; MH; Marker-heterozygous flies (mwh +/+ flare-3) with phenotypically wild-type wings; Metofluthrin; SMART; ST cross; Somatic Mutation and Recombination Test; Standard cross; Transfluthrin; Wing spot test; c; flare-3 (Drosophila strain); flr(3); m; multiple wing hairs (Drosophila strain); multiplication factor; mwh