title: Clinical significance of Ankyrin repeat domain 12 expression in colorectal cancer.
abstract: BACKGROUND
  Ankyrin repeat domain 12 (ANKRD12), is encoding a 224 kDa nuclear protein and most conserved at its N-terminal ankyrin repeats region and the C-terminal co-activator interacting domain. The aim of this study was to investigate the ANKRD12 mRNA expression in colorectal cancer (CRC) tumor tissues and the normal adjacent mucosa and its potential relevance to clinicopathological characteristics and prognosis.
  
  
  METHODS
  Surgical specimens of tumor tissues (n = 68) and adjacent normal mucosa (n = 51) were obtained from CRC patients. The ANKRD12 mRNA expression was measured by quantitative real time reverse transcriptase polymerase chain reaction. The relationship between ANKRD12 mRNA expression and clinicopathological features was analyzed by appropriate statistics. Kaplan-Meier analysis and Cox proportional hazards regression models were used to investigate the correlation between ANKRD12 expression and prognosis of CRC patients.
  
  
  RESULTS
  The relative mRNA expression of ANKRD12 were significantly lower in CRC tumor tissues than in the normal adjacent mucosa (P < 0.001), and the cases with low ANKRD12 expression showed a higher frequency of liver metastasis (P = 0.015). Kaplan-Meier analysis indicated that patients (CRC without liver metastasis) with low ANKRD12 expression had poor overall survival (P = 0.041). Multivariate analysis showed that low ANKRD12 expression was an independent predictor of overall survival.
  
  
  CONCLUSIONS
  This study revealed that ANKRD12 mRNA were down regulated in CRC tumor tissues and low ANKRD12 expression was correlated with liver metastasis and poor survival of CRC patients.
authors.forename: Rui
authors.initials: R
authors.lastname: Bai
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Zhong
authors.initials: Z
authors.lastname: Shi
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Fang
authors.forename: Weiting
authors.initials: W
authors.lastname: Ge
authors.forename: Shu
authors.initials: S
authors.lastname: Zheng
mesh_terms: D017089:Ankyrin Repeat; D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000235:genetics; D011379:Prognosis; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
chemical_list: C000596750:ANKRD12 protein, human; D014408:Biomarkers, Tumor; D009687:Nuclear Proteins; D012333:RNA, Messenger