title: Lumican as a novel marker for differential diagnosis of Bowen disease and actinic keratosis.
abstract: Lumican, a member of the small leucine-rich proteoglycan family, regulates the assembly and diameter of collagen fibers in the extracellular matrix of various tissues. Lumican expression correlates with pathological conditions, including skin fragility, corneal opacification, and corneal and cardiac wound healing. Lumican is overexpressed in tumor cells, including in the breast, colorectal, neuroendocrine cell, uterine cervical, and pancreatic cancers. Lumican expression also correlates with the growth and metastasis of various malignancies. For example, lumican expression is lower in the dermis of malignant melanoma cases than in early-stage melanomas. However, the expression patterns and roles of lumican in nonmelanoma skin cancer have not been elucidated. In this study, we used immunohistochemistry and in situ hybridization to examine the expression patterns of lumican in normal skin, Bowen disease, and actinic keratosis. In normal skin, lumican was expressed in the collagen fibers in the dermis, acrosyringium, follicular epithelium, and sebocytes but not in epidermal keratinocytes. In Bowen disease, lumican was expressed in 34 (91.8%) of 37 patients. Notably, all cases of actinic keratosis were negative for lumican. These findings suggest that lumican plays an important role in the pathogenesis of Bowen disease and actinic keratosis and might be useful as an adjunct to the diagnosis for subtypes of 2 diseases: bowenoid actinic keratosis and Bowen disease in sun-exposed areas.
authors.affiliation: *Department of Dermatology; †Department of Pathology; and ‡Department of Integrative Oncological Pathology, Nippon Medical School, Tokyo, Japan.
authors.forename: Ryoko
authors.initials: R
authors.lastname: Takayama
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Ishiwata
authors.forename: Shin-ichi
authors.initials: S
authors.lastname: Ansai
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Yamamoto
authors.forename: Yoko
authors.initials: Y
authors.lastname: Matsuda
authors.forename: Zenya
authors.initials: Z
authors.lastname: Naito
authors.forename: Seiji
authors.initials: S
authors.lastname: Kawana
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000032:analysis; D001913:Bowen's Disease / Q000175:diagnosis*; D011508:Chondroitin Sulfate Proteoglycans / Q000032:analysis*; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D007632:Keratan Sulfate / Q000032:analysis*; D055623:Keratosis, Actinic / Q000175:diagnosis*; D000071234:Lumican; D008297:Male; D008875:Middle Aged; D011230:Precancerous Conditions / Q000175:diagnosis*; D012878:Skin Neoplasms / Q000175:diagnosis*
chemical_list: D015415:Biomarkers; D011508:Chondroitin Sulfate Proteoglycans; C000604306:LUM protein, human; D000071234:Lumican; D007632:Keratan Sulfate