title: In vitro exposure to cigarette smoke induces oxidative stress in follicular cells of F₁ hybrid mice.
abstract: This study assessed the influence of cigarette smoke condensate (CSC) and benzo(a)pyrene [B(a)P] on the levels of two oxidative stress biomarkers [8-isoprostane (8-IsoP) and 8-hydroxy-2-deoxy Guanosine (8-OH-dG)], in in-vitro spent media of follicle cells. Follicles (100-130 µm) isolated from ovaries of F₁ hybrid (C57Bl/6j × CBA/Ca) mice were cultured for 13 days in media exposed to B(a)P [0 ng ml⁻¹ (control) to 45 ng ml⁻¹] or CSC [0 µg ml⁻¹ (control) to 130 µg ml⁻¹]. The concentrations of oxidative stress biomarkers in spent media were quantified by enzyme-linked immune sorbent assays (ELISA). CSC and B(a)P treatment induced a significant, dose-dependent increase in the concentrations of 8-IsoP and 8-OH-dG in the spent media. We conclude that CSC and B(a)P exposure can induce oxidative stress in ovarian follicles, an effect that may contribute to the previously documented decline in follicle development and premature ovarian failure in women who smoke.
authors.affiliation: Exposure and Biomonitoring Division, Health Canada, Ottawa, Ontario, Canada.
authors.forename: Shabana
authors.initials: S
authors.lastname: Siddique
authors.forename: Jean C
authors.initials: JC
authors.lastname: Sadeu
authors.forename: Warren G
authors.initials: WG
authors.lastname: Foster
authors.forename: Yong-Lai
authors.initials: YL
authors.lastname: Feng
authors.forename: Jiping
authors.initials: J
authors.lastname: Zhu
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity; D002478:Cells, Cultured; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000032:analysis; D015237:Dinoprost / Q000031:analogs & derivatives / Q000032:analysis; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D006080:Ovarian Follicle / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D012906:Smoke / Q000009:adverse effects*
chemical_list: D012906:Smoke; C075750:8-epi-prostaglandin F2alpha; D001564:Benzo(a)pyrene; D000080242:8-Hydroxy-2'-Deoxyguanosine; D015237:Dinoprost; D003849:Deoxyguanosine
keywords: ELISA; benzo(a)pyrene; follicle cell growth; oxidative stress biomarkers