title: Preparation and characterization of a new microwave immobilized poly(2-phenylpropyl)methylsiloxane stationary phase for reversed phase high-performance liquid chromatography.
abstract: A new reversed phase high-performance liquid chromatography (RP-HPLC) stationary phase was prepared and its chromatographic and physical-chemical properties were evaluated. The new stationary phase was prepared with a silica support and poly(2-phenylpropyl)methylsiloxane (PPPMS), a phenyl type polysiloxane copolymer. Since this is a new copolymer and there is little information in the literature, it was submitted to physical-chemical characterization by infrared spectroscopy and thermogravimetry. The chromatographic phase was prepared through sorption and microwave immobilization of the copolymer onto a silica support. The chromatographic performance was evaluated by employing test procedures suggested by Engelhardt and Jungheim, Tanaka and co-workers, Neue, and Szabó and Csató. These test mixtures provide information about the hydrophobic selectivity, silanophilic activity, ion-exchange capacity, shape selectivity and interaction with polar analytes of the new Si-PPPMS reversed phase. Stability tests were developed using accelerated aging tests under both basic and acidic conditions to provide information about the lifetime of the packed columns.
authors.affiliation: Instituto de Química, Universidade Estadual de Campinas, Caixa Postal 6154, CEP 13084-971, Campinas, SP, Brazil.
authors.forename: Fernanda R
authors.initials: FR
authors.lastname: Begnini
authors.forename: Isabel C S F
authors.initials: IC
authors.lastname: Jardim
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods; D056148:Chromatography, Reverse-Phase / Q000295:instrumentation* / Q000379:methods; D007554:Isotopes / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008872:Microwaves; D012825:Silicon / Q000737:chemistry; D012833:Siloxanes / Q000737:chemistry*
chemical_list: D007554:Isotopes; D012833:Siloxanes; D012825:Silicon