title: Interleukin-10 gene promoter polymorphisms and risk of gastric cancer in a Chinese population: single nucleotide and haplotype analyses.
abstract: OBJECTIVE
  Interleukin (IL) -10 is a potent cytokine with a dual ability to immunosuppress or immunostimulate. We aimed to explore the association of IL10 promoter polymorphisms with risk of gastric cancer (GC) in a Han population in Southwestern China.
  
  
  METHODS
  We enrolled 308 pairs of GC and control subjects from four hospitals and a community between October 2010 and August 2011 in a 1:1 matched case-control design. Demographic information was collected using a designed questionnaire. IL10-592 A>C and IL10-1082 A>G polymorphisms were determined by Sequenom MassARRAY analysis.
  
  
  RESULTS
  Patients with GC reported statistically higher proportions of family history of cancer (29.9% versus 10.7%, P<0.01) and alcohol drinking (54.6% versus 43.2%, P<0.01) than did controls. Similar results were observed in comparison between non-cardia GC patients and controls (P<0.01 and P=0.03). Variant genotypes of IL10-592 A>C and IL10-1082 A>G were not associated with overall GC risk (adjusted OR, 0.94, 95% CI, 0.66-1.33; adjusted OR, 1.00, 95% CI, 0.62-1.60). Sub-analysis showed that the IL10-592 AC/CC variant genotype was associated with decreased non-cardia GC risk (adjusted OR, 0.58; 95% CI, 0.36-0.95). No association was found between any of the IL10 haplotypes established from two polymorphisms and risk of non-cardia GC.
  
  
  CONCLUSIONS
  In conclusion, our data do not link the two SNPs of IL10-592 and IL10-1082 with overall GC risk. We demonstrate that IL10-592 polymorphism is associated with protective effect against non-cardia GC. Our findings may offer insight into risk associated with the development of GC in this region.
authors.affiliation: Department of Epidemiology, West China School of Public Health, Sichuan University, Chengdu, Sichuan, China.
authors.forename: Xiong-Fei
authors.initials: XF
authors.lastname: Pan
authors.forename: Shu-Juan
authors.initials: SJ
authors.lastname: Yang
authors.forename: Marie
authors.initials: M
authors.lastname: Loh
authors.forename: Yao
authors.initials: Y
authors.lastname: Xie
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Wen
authors.forename: Zhi
authors.initials: Z
authors.lastname: Tian
authors.forename: He
authors.initials: H
authors.lastname: Huang
authors.forename: Hui
authors.initials: H
authors.lastname: Lan
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
authors.forename: Richie
authors.initials: R
authors.lastname: Soong
authors.forename: Chun-Xia
authors.initials: CX
authors.lastname: Yang
mesh_terms: D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D005260:Female; D005500:Follow-Up Studies; D005838:Genotype; D006239:Haplotypes / Q000235:genetics*; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011379:Prognosis; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012307:Risk Factors; D013274:Stomach Neoplasms / Q000209:etiology* / Q000473:pathology; D015996:Survival Rate
chemical_list: D016753:Interleukin-10