title: [Progress in microbial production of alpha-ketoglutarate].
abstract: Alpha-ketogluratate is one of the key intermediates in the TCA cycle, playing an important role in the connection of carbon and nitrogen metabolism. This article aims at stating recent research progress in the production of alpha-ketoglutarate by microbial fermentation. First, a large group of microbes have been screened to accumulate alpha-ketoglutarate including prokaryotes and eukaryotes. Second, physiological characterization of over-accumulation of alpha-ketoglutarate is caused by thiamine defect and nitrogen starvation. Third, the process of fermentation was controlled and optimized by the manipulation of pH, dissolved oxygen and cofactors. Fourth, many metabolic engineering strategies were also presented for alpha-ketoglutarate production focusing on regeneration of cofactor and manipulation of the pathway. Last, we discussed the limitation of current progress and proposed the future research needs for microbial production of alpha-ketoglutarate.
authors.affiliation: Key Laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Guo
authors.forename: Guocheng
authors.initials: G
authors.lastname: Du
authors.forename: Jingwen
authors.initials: J
authors.lastname: Zhou
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
mesh_terms: D001419:Bacteria / Q000254:growth & development / Q000378:metabolism*; D005285:Fermentation; D005658:Fungi / Q000254:growth & development / Q000378:metabolism; D007218:Industrial Microbiology / Q000379:methods; D007656:Ketoglutaric Acids / Q000378:metabolism*; D060847:Metabolic Engineering; D025062:Yarrowia / Q000254:growth & development / Q000378:metabolism*
chemical_list: D007656:Ketoglutaric Acids