title: Chemiluminescence determination of moxifloxacin in pharmaceutical and biological samples based on its enhancing effect of the luminol-ferricyanide system using a microfluidic chip.
abstract: A sensitive determination of a synthetic fluoroquinolone antibacterial agent, moxifloxacin (MOX), by an enhanced chemiluminescence (CL) method using a microfluidic chip is described. The microfluidic chip was fabricated by a soft-lithographic procedure using polydimethyl siloxane (PDMS). The fabricated PDMS microfluidic chip had three-inlet microchannels for introducing the sample, chemiluminescent reagent and oxidant, and a 500 µm wide, 250 µm deep and 82 mm long microchannel. An enhanced CL system, luminol-ferricyanide, was adopted to analyze the MOX concentration in a sample solution. CL light was emitted continuously after mixing luminol and ferricyanide in the presence of MOX on the PDMS microfluidic chip. The amount of MOX in the luminol-ferricyanide system influenced the intensity of the CL light. The linear range of MOX concentration was 0.14-55.0 ng/mL with a correlation coefficient of 0.9992. The limit of detection (LOD) and limit of quantification (LOQ) were 0.06 and 0.2 ng/mL respectively. The presented method afforded good reproducibility, with a relative standard deviation (RSD) of 1.05% for 10 ng/mL of MOX, and has been successfully applied for the determination of MOX in pharmaceutical and biological samples.
authors.affiliation: Department of Chemistry, Kyungpook National University, Daegu, 702-701, South Korea.
authors.forename: Yeoun Suk
authors.initials: YS
authors.lastname: Suh
authors.forename: Mohammad
authors.initials: M
authors.lastname: Kamruzzaman
authors.forename: Al-Mahmnur
authors.initials: AM
authors.lastname: Alam
authors.forename: Sang Hak
authors.initials: SH
authors.lastname: Lee
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
authors.forename: Gyu-Man
authors.initials: GM
authors.lastname: Kim
authors.forename: Trung Dung
authors.initials: TD
authors.lastname: Dang
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000652:urine; D024841:Fluoroquinolones / Q000032:analysis* / Q000652:urine; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000737:chemistry*; D044085:Microfluidics / Q000379:methods*; D000077266:Moxifloxacin; D013607:Tablets / Q000032:analysis
chemical_list: D000900:Anti-Bacterial Agents; D024841:Fluoroquinolones; D013607:Tablets; D008165:Luminol; D000077266:Moxifloxacin
keywords: chemiluminescence; ferricyanide; luminol; microfluidic chip; moxifloxacin