title: Depth profile analysis of non-specific fluorescence and color of tooth tissues after peroxide bleaching.
abstract: OBJECTIVE
  To examine laboratory changes of endogenous non-specific fluorescence and color throughout subsurface of tooth structures prior to and following peroxide bleaching.
  
  
  METHODS
  Extracted human teeth were cross sectioned and mounted on glass slides. Cross sections were examined for internal color (digital camera) and nonspecific fluorescence (microRaman spectroscopy) throughout the tooth structure at specified locations. Surfaces of sections were then saturation bleached for 70 hours with a gel containing 6% hydrogen peroxide. Cross sections were reexamined for color and non-specific fluorescence changes.
  
  
  RESULTS
  Unbleached enamel, dentin-enamel junction and dentin exhibit different CIELab color and non-specific fluorescence properties. Bleaching of teeth produced significant changes in color of internal cross sections and substantial reductions of non-specific fluorescence levels within enamel dentin and DEJ. Enamel and dentin non-specific fluorescence were reduced to common values with bleaching with enamel and the DEJ showing larger reductions than dentin.
authors.affiliation: Health Care Research Center, The Procter and Gamble Company, 8700 Mason Montgomery Road, Mason, OH 45040, USA.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Klukowska
authors.forename: Hermann
authors.initials: H
authors.lastname: Götz
authors.forename: Donald J
authors.initials: DJ
authors.lastname: White
authors.forename: James
authors.initials: J
authors.lastname: Zoladz
authors.forename: Björn-Olaf
authors.initials: BO
authors.lastname: Schwarz
authors.forename: Heinz
authors.initials: H
authors.lastname: Duschner
mesh_terms: D003116:Color; D003743:Dental Enamel / Q000033:anatomy & histology / Q000187:drug effects; D003804:Dentin / Q000033:anatomy & histology / Q000187:drug effects; D005453:Fluorescence; D006801:Humans; D006861:Hydrogen Peroxide / Q000627:therapeutic use*; D015591:Microspectrophotometry / Q000379:methods; D013059:Spectrum Analysis, Raman / Q000379:methods; D013997:Time Factors; D014070:Tooth / Q000033:anatomy & histology / Q000187:drug effects*; D014073:Tooth Bleaching / Q000379:methods*; D058205:Tooth Bleaching Agents / Q000627:therapeutic use*
chemical_list: D058205:Tooth Bleaching Agents; D006861:Hydrogen Peroxide