title: Divergent immune responses to Mycobacterium avium subsp. paratuberculosis infection correlate with kinome responses at the site of intestinal infection.
abstract: Mycobacterium avium subsp. paratuberculosis is the causative agent of Johne's disease (JD) in cattle. M. avium subsp. paratuberculosis infects the gastrointestinal tract of calves, localizing and persisting primarily in the distal ileum. A high percentage of cattle exposed to M. avium subsp. paratuberculosis do not develop JD, but the mechanisms by which they resist infection are not understood. Here, we merge an established in vivo bovine intestinal segment model for M. avium subsp. paratuberculosis infection with bovine-specific peptide kinome arrays as a first step to understanding how infection influences host kinomic responses at the site of infection. Application of peptide arrays to in vivo tissue samples represents a critical and ambitious step in using this technology to understand host-pathogen interactions. Kinome analysis was performed on intestinal samples from 4 ileal segments subdivided into 10 separate compartments (6 M. avium subsp. paratuberculosis-infected compartments and 4 intra-animal controls) using bovine-specific peptide arrays. Kinome data sets clustered into two groups, suggesting unique binary responses to M. avium subsp. paratuberculosis. Similarly, two M. avium subsp. paratuberculosis-specific immune responses, characterized by different antibody, T cell proliferation, and gamma interferon (IFN-γ) responses, were also observed. Interestingly, the kinomic groupings segregated with the immune response groupings. Pathway and gene ontology analyses revealed that differences in innate immune and interleukin signaling and particular differences in the Wnt/β-catenin pathway distinguished the kinomic groupings. Collectively, kinome analysis of tissue samples offers insight into the complex cellular responses induced by M. avium subsp. paratuberculosis in the ileum and provides a novel method to understand mechanisms that alter the balance between cell-mediated and antibody responses to M. avium subsp. paratuberculosis infection.
authors.affiliation: Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
authors.forename: Pekka
authors.initials: P
authors.lastname: Määttänen
authors.forename: Brett
authors.initials: B
authors.lastname: Trost
authors.forename: Erin
authors.initials: E
authors.lastname: Scruten
authors.forename: Andrew
authors.initials: A
authors.lastname: Potter
authors.forename: Anthony
authors.initials: A
authors.lastname: Kusalik
authors.forename: Philip
authors.initials: P
authors.lastname: Griebel
authors.forename: Scott
authors.initials: S
authors.lastname: Napper
mesh_terms: D000818:Animals; D002417:Cattle; D004195:Disease Models, Animal; D004591:Electrophoresis, Polyacrylamide Gel; D054884:Host-Pathogen Interactions / Q000276:immunology; D006801:Humans; D007113:Immunity, Innate / Q000276:immunology; D007413:Intestinal Mucosa / Q000378:metabolism / Q000382:microbiology*; D007422:Intestines / Q000382:microbiology; D046228:Microarray Analysis; D009093:Mucus / Q000378:metabolism; D016927:Mycobacterium avium subsp. paratuberculosis / Q000276:immunology* / Q000378:metabolism / Q000472:pathogenicity; D010283:Paratuberculosis / Q000276:immunology* / Q000378:metabolism / Q000382:microbiology*; D010770:Phosphotransferases / Q000096:biosynthesis; D059467:Transcriptome
chemical_list: D010770:Phosphotransferases