title: Bioassay-guided evaluation of wound healing effect of fatty acids-incorporated collagen-based films.
abstract: OBJECTIVE
  To evaluate the effects of fatty acids-incorporated collagen-based dressing films on wound healing in rodents.
  
  
  METHODS
  Therefore, surgical wounds were performed in the back of 80 Wistar rats, and dressed with collgane-based films (COL), and collagen-based films containing fatty acids (AGEF50 and AGEF100). Undressed wounds were regarded as controls (CTR). The animals were euthanized after three, seven, 14 and 21 days, and the macroscopic wound contraction rates (WRC) were assessed. The wounded area was also analyzed by conventional and polarized light microscope.
  
  
  RESULTS
  No sign of abscess or hypertrophic scar formation was observed in none of the groups. At seven days, the WRR of AGEF50 was significantly higher than CTR (p<0.01), whereas at 14 days, both AGE 50 and AGE100 showed a significant increase of the WRR compared to CTR (p<0.001) and COL (p<0.01). Both films promoted increased influx of neutrophils at three days (p<0.01), but reduced significantly the mononuclear infiltrate at 14 days (p<0.05). It was also observed earlier maturation of the granulation tissue, full epithelization and cutaneous appendages development, as well as better collagenization, in AGEF50 and AGEF100.
  
  
  CONCLUSIONS
  The application of AGEF50/100 as wound dressing improved wound healing in rodents.
authors.affiliation: Laboratory of Biomaterials, Department of Pharmacy, University Tiradentes, Aracaju, SE, Brazil.
authors.forename: Catharina Grace
authors.initials: CG
authors.lastname: Santos
authors.forename: Marismar Fernandes do
authors.initials: MF
authors.lastname: Nascimento
authors.forename: Clauberto Rodrigues de
authors.initials: CR
authors.lastname: Oliveira
authors.forename: Genecy Calado de
authors.initials: GC
authors.lastname: Melo
authors.forename: Juliana Cordeiro
authors.initials: JC
authors.lastname: Cardoso
authors.forename: Francine Ferreira
authors.initials: FF
authors.lastname: Padilha
authors.forename: Sonia Oliveira
authors.initials: SO
authors.lastname: Lima
authors.forename: Ricardo Luiz Cavalcanti de
authors.initials: RL
authors.lastname: Albuquerque-Júnior
mesh_terms: D000818:Animals; D001458:Bandages; D001681:Biological Assay; D003094:Collagen / Q000737:chemistry / Q000627:therapeutic use*; D004195:Disease Models, Animal; D005227:Fatty Acids / Q000737:chemistry / Q000627:therapeutic use*; D007249:Inflammation / Q000473:pathology; D008297:Male; D008422:Materials Testing; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D016896:Treatment Outcome; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D005227:Fatty Acids; D003094:Collagen