title: High-fat feeding rapidly induces obesity and lipid derangements in C57BL/6N mice.
abstract: C57BL/6N (B6N) is becoming the standard background for genetic manipulation of the mouse genome. The B6N, whose genome is very closely related to the reference C57BL/6J genome, is versatile in a wide range of phenotyping and experimental settings and large repositories of B6N ES cells have been developed. Here, we present a series of studies showing the baseline characteristics of B6N fed a high-fat diet (HFD) for up to 12 weeks. We show that HFD-fed B6N mice show increased weight gain, fat mass, and hypercholesterolemia compared to control diet-fed mice. In addition, HFD-fed B6N mice display a rapid onset of lipid accumulation in the liver with both macro- and microvacuolation, which became more severe with increasing duration of HFD. Our results suggest that the B6N mouse strain is a versatile background for studying diet-induced metabolic syndrome and may also represent a model for early nonalcoholic fatty liver disease.
authors.affiliation: Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, UK.
authors.forename: Christine
authors.initials: C
authors.lastname: Podrini
authors.forename: Emma L
authors.initials: EL
authors.lastname: Cambridge
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Lelliott
authors.forename: Damian M
authors.initials: DM
authors.lastname: Carragher
authors.forename: Jeanne
authors.initials: J
authors.lastname: Estabel
authors.forename: Anna-Karin
authors.initials: AK
authors.lastname: Gerdin
authors.forename: Natasha A
authors.initials: NA
authors.lastname: Karp
authors.forename: Cheryl L
authors.initials: CL
authors.lastname: Scudamore
authors.forename: Ramiro
authors.initials: R
authors.lastname: Ramirez-Solis
authors.forename: Jacqueline K
authors.initials: JK
authors.lastname: White
mesh_terms: D000818:Animals; D001835:Body Weight; D059305:Diet, High-Fat / Q000009:adverse effects; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D050356:Lipid Metabolism; D008099:Liver / Q000378:metabolism; D008297:Male; D051379:Mice / Q000254:growth & development / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D009765:Obesity / Q000209:etiology / Q000378:metabolism*