title: [Comparison of activated carbon and ultrafiltration technique in the production process of huoxue tongluo injection].
abstract: OBJECTIVE
  To research the applicability of activated carbon and ultrafiltration technique in the production process of Huoxue Tongluo Injection.
  
  
  METHODS
  The kinetic-turbidimetric method was used to determine the content of bacterial endotoxins in Huoxue Tongluo solution. Particle size change in Huoxue Tongluo solution was determined by nanometer particle size instrument before and after the use of different concentration activated carbon and different molecular weight ultrafiltration membrane.
  
  
  RESULTS
  The removal efficacy of bacterial endotoxins was 65.2%, 77.5%, 80.4% by using three concentrations of active carbon at 0.05%, 0.10%, 0.30% in Huoxue Tongluo Injection, respectively. It was above 95% by using cutoff molecular weight both 5 kDa and 10 kDa ultrafiltration membrane. Measure results by nanometer particle size instrument showed that particle size of filter liquor by 10 kDa cutoff molecular weight ultrafiltration membrane was much smaller than that of by use of different concentration activated carbon.
  
  
  CONCLUSIONS
  Ultrafiltration method is more suitable to the removal of bacterial endotoxins. The solution is more clear after using ultrafiltration method, and large particles of solution is removed. The ultrafiltration method provides the basis for injection production.
authors.affiliation: School of Pharmacy of Nanjing University of Chinese Medicine, Nanjing 210046, China. lihemin2006@sina.com
authors.forename: He-Min
authors.initials: HM
authors.lastname: Li
authors.forename: Yun-Feng
authors.initials: YF
authors.lastname: Zheng
authors.forename: Xing-Lei
authors.initials: XL
authors.lastname: Zhi
authors.forename: Cun-Yu
authors.initials: CY
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Jiang
authors.forename: Guo-Ping
authors.initials: GP
authors.lastname: Peng
mesh_terms: D000327:Adsorption; D002606:Charcoal / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D004731:Endotoxins / Q000032:analysis / Q000302:isolation & purification*; D007267:Injections; D008567:Membranes, Artificial; D008970:Molecular Weight; D010316:Particle Size; D014462:Ultrafiltration / Q000379:methods; D014865:Waste Disposal, Fluid / Q000379:methods*
chemical_list: D004365:Drugs, Chinese Herbal; D004731:Endotoxins; D008567:Membranes, Artificial; C527174:tongluo; D002606:Charcoal