title: In vitro effects of SB202190 on Echinococcus granulosus.
abstract: Spillage of cyst contents during surgical operation is the major cause of recurrence after hydatid cyst surgery. Instillation of a scolicidal agent into a hepatic hydatid cyst is the most commonly employed measure to prevent this complication. SB202190 is a pyridinyl imidazole derivative and is known to be a specific inhibitor of p38 MAPK. In the present study, the scolicidal effect of SB202190 was investigated. Freshly isolated Echinococcus granulosus protoscolices were subjected to SB202190 treatment (10, 20, 40, and 80 µM), and the effects on parasite viability were monitored by trypan blue staining. Corresponding effects were visualized by scanning and transmission electron microscopy. Dose-dependent protoscolex death within a few days of SB202190 treatment was observed. Although the in vitro scolicidal effect of SB202190 was satisfactory, the in vivo efficacy of this drug and also possible side effects remain to be further investigated.
authors.affiliation: Department of Hepatobiliary Surgery, The First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang, China.
authors.forename: Hailong
authors.initials: H
authors.lastname: Lv
authors.forename: Siyuan
authors.initials: S
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Weihua
authors.initials: W
authors.lastname: Liang
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Mu
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D048209:Echinococcus granulosus / Q000187:drug effects* / Q000648:ultrastructure; D007093:Imidazoles / Q000494:pharmacology*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D021261:Parasitic Sensitivity Tests; D011725:Pyridines / Q000494:pharmacology*; D016019:Survival Analysis
chemical_list: D000871:Anthelmintics; D007093:Imidazoles; D011725:Pyridines; C090942:4-(4-fluorophenyl)-2-(4-hydroxyphenyl)-5-(4-pyridyl)imidazole
keywords: Echinococcus granulosus; SB202190; hydatid cyst; protoscolex