title: Rapid discrimination of Acinetobacter baumannii international clone II lineage by pyrosequencing SNP analyses of bla(OXA-51-like) genes.
abstract: We found that Acinetobacter baumannii international clone II generally possesses unique GTA sequence at nucleotide positions 106-108 in the bla(OXA-51-like) genes. We exploited this to develop an easy and rapid method for discrimination of international clone II from other A. baumannii by employing pyrosequencing analyses of single nucleotide polymorphisms.
authors.affiliation: Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan. Electronic address: m-matsui@nih.go.jp.
authors.forename: Mari
authors.initials: M
authors.lastname: Matsui
authors.affiliation: Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Satowa
authors.initials: S
authors.lastname: Suzuki
authors.affiliation: Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Suzuki
authors.affiliation: Department of Bacteriology, Nagoya University Graduate School of Medicine, Nagoya Japan.
authors.forename: Yoshichika
authors.initials: Y
authors.lastname: Arakawa
authors.affiliation: Department of Bacteriology II, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Keigo
authors.initials: K
authors.lastname: Shibayama
mesh_terms: D040981:Acinetobacter baumannii / Q000145:classification / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D015373:Bacterial Typing Techniques / Q000379:methods*; D001483:Base Sequence; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D008969:Molecular Sequence Data; D010802:Phylogeny; D020641:Polymorphism, Single Nucleotide; D001618:beta-Lactamases / Q000235:genetics
chemical_list: D001618:beta-Lactamases; C577373:beta-lactamase OXA-51, Acinetobacter baumannii
keywords: Acinetobacter baumannii; Pyrosequence; bla(OXA-51-like)