title: Environment acidity triggers release of recombinant adeno-associated virus serotype 2 from a tunable matrix.
abstract: Successful design of a pH responsive polyelectrolyte-based virus delivery matrix with extracellular release triggered by tumor acidosis has been achieved. Recombinant adeno-associated virus serotype 2 (AAV2) is loaded in the polyelectrolyte-based matrix (AAV2-matrix), which is formed by a biodegradable copolymer of poly(polyethylene glycol-1-(3-aminopropyl)imidazole-dl-aspartic acid) with tuned pH response based on inclusion of polyethyleneimine (PEI(800)). Physico-chemical properties of AAV2-matrix are optimized to minimize cellular interactions until a tumor acidosis-like environment protonates the matrix, reverses ζ-potential and causes particles to swell, releasing the AAV2 virus. The pH-dependent release is highly controllable and potentially useful to optimize site specific viral delivery.
authors.affiliation: Institute of Physics, Academia Sinica, Nankang, Taipei 11529, Taiwan.
authors.forename: S-Ja
authors.initials: SJ
authors.lastname: Tseng
authors.forename: Ivan M
authors.initials: IM
authors.lastname: Kempson
authors.forename: Shu-Fen
authors.initials: SF
authors.lastname: Peng
authors.forename: Bai-Hung
authors.initials: BH
authors.lastname: Ke
authors.forename: Hsiang-Hsin
authors.initials: HH
authors.lastname: Chen
authors.forename: Pei-Feng
authors.initials: PF
authors.lastname: Chen
authors.forename: Y
authors.initials: Y
authors.lastname: Hwu
mesh_terms: D000818:Animals; D002470:Cell Survival; D000229:Dependovirus; D049452:Green Fluorescent Proteins / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D051379:Mice; D008819:Mice, Nude; D041681:NIH 3T3 Cells; D009369:Neoplasms / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry; D014161:Transduction, Genetic
chemical_list: D011108:Polymers; D049452:Green Fluorescent Proteins
keywords: Gene delivery; Localized delivery; Recombinant adeno-associated virus; Transduction; pH-sensitive vector