title: Hemocompatible and antibacterial porous membranes with heparinized copper hydroxide nanofibers as separation layer.
abstract: Here we report the fabrication of a novel heparinized copper hydroxide (Cu(OH)2) nanofiberous membrane with satisfying hemocompatibility and antibacterial properties. The positively charged Cu(OH)2 nanofibers were prepared in a weakly alkaline copper nitrate solution in the presence of 2-aminoethanol. A heparin (Hep) solution was then added dropwise into the solution of nanofibers to immobilize negatively charged heparin onto the Cu(OH)2 nanofibers by electrostatic interaction. A composite Hep@Cu(OH)2 nanofiberous membrane was prepared by filtration and deposition of the heparinized nanofibers onto a polysulfone (PSF) porous membrane. Chemical composition analysis of membrane surface using X-ray photoelectron spectroscopy (XPS) confirmed the successful immobilization of heparin on Cu(OH)2 nanofibers. The amount of immobilized heparin on nanofiberous membrane was determined by a colorimetric assay of toluidine blue dye and the results showed that the amount of immobilized heparin was strongly dependent on the heparin dosage in reaction solution. The results of contact angle measurement indicated that the hydrophilicity of Cu(OH)2 nanofiberous membranes was enhanced by the immobilization of heparin. The adhesion, activation and transmutation of platelets on Hep@Cu(OH)2 membrane were suppressed remarkably due to the introduction of heparin, which suggested that the Hep@Cu(OH)2 membranes had good hemocompatibility. In addition, Cu(OH)2 and Hep@Cu(OH)2 nanofiberous membranes exhibited very good antibacterial activities against Escherichia coli and Staphyloccocus aureus.
authors.affiliation: MOE Key Laboratory of Macromolecule Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, PR China.
authors.forename: Li-Jing
authors.initials: LJ
authors.lastname: Zhu
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Zhu
authors.forename: Zhuan
authors.initials: Z
authors.lastname: Yi
authors.forename: Jin-Hong
authors.initials: JH
authors.lastname: Jiang
authors.forename: Bao-Ku
authors.initials: BK
authors.lastname: Zhu
authors.forename: You-Yi
authors.initials: YY
authors.lastname: Xu
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry*; D003300:Copper / Q000737:chemistry*; D006493:Heparin / Q000737:chemistry*; D006878:Hydroxides / Q000737:chemistry*; D057139:Nanofibers / Q000737:chemistry*; D010316:Particle Size; D016062:Porosity; D013499:Surface Properties
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D006878:Hydroxides; C508959:copper hydroxide; D003300:Copper; D006493:Heparin