title: Flow-injection chemiluminescence determination of olanzapine using N-chlorosuccinimide-calcein reaction sensitized by zinc (II).
abstract: A novel, rapid and sensitive chemiluminescence (CL) method combined with flow-injection (FI) has been established for the estimation of olanzapine. This method is based on the CL signal generated between N-chlorosuccinimide and olanzapine in an alkaline medium in the presence of calcein and Zn(II). Under optimum conditions, the CL signal was proportional to the olanzapine concentration ranging from 1.0 × 10(-10) to 3.0 × 10(-7) g/mL. The detection limit is 8.9 × 10(-11) g/mL olanzapine (3σ) and the relative standard deviation for 3.0 × 10(-9) g/mL of olanzapine is 1.9% (n = 11). The current CL method was applied to determine olanzapine in pharmaceutical formulations and biological fluids with satisfactory results. The possible CL reaction mechanism is discussed briefly.
authors.affiliation: School of Chemistry and Chemical Engineering, Shihezi University, Shihezi, Xinjiang, 832003, People's Republic of China.
authors.forename: Fang
authors.initials: F
authors.lastname: Zhao
authors.forename: Qi
authors.initials: Q
authors.lastname: Fan
authors.forename: Huan
authors.initials: H
authors.lastname: Cai
mesh_terms: D014150:Antipsychotic Agents / Q000097:blood*; D001569:Benzodiazepines / Q000097:blood*; D017022:Flow Injection Analysis / Q000379:methods*; D005452:Fluoresceins / Q000737:chemistry*; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D000077152:Olanzapine; D013388:Succinimides / Q000737:chemistry*; D015032:Zinc / Q000737:chemistry*
chemical_list: D014150:Antipsychotic Agents; D005452:Fluoresceins; D013388:Succinimides; C015752:N-chlorosuccinimide; D001569:Benzodiazepines; D015032:Zinc; D000077152:Olanzapine; C007740:fluorexon
keywords: calcein; chemiluminescence; flow-injection; olanzapine; zinc (II) ion