title: Purification and characterization of a 1,3-β-D-glucan recognition protein from Antheraea pernyi larve that is regulated after a specific immune challenge.
abstract: Pattern recognition receptors are known to participate in the activation of Prophenoloxidase system. In this study, a 1,3-β-D-glucan recognition protein was detected for the first time in Antheraea pernyi larvae (Ap-βGRP). Ap-βGRP was purified to 99.9% homogeneity from the hemolymph using traditional chromatographic methods. Ap-βGRP specifically bind 1,3-β-D-glucan and yeast, but not E. coli or M. luteus. The 1,3-β-D-glucan dependent phenoloxidase (PO) activity of the hemolymph inhibited by anti-Ap-βGRP antibody could be recovered by addition of purified Ap-βGRP. These results demonstrate that Ap-βGRP acts as a biosensor of 1,3-β-Dglucan to trigger the Prophenoloxidase system. A trace mount of 1,3-β-D-glucan or Ap-βGRP alone was unable to trigger the proPO system, but they both did. Ap-βGRP was specifically degraded following the activation of proPO with 1,3-β-Dglucan. These results indicate the variation in the amount of Ap-βGRP after specific immune challenge in A. pernyi hemolymph is an important regulation mechanism to immune response.
authors.affiliation: School of Life Science and Bio-pharmaceutics, Shenyang Pharmaceutical University, Shenyang, Liaoning Province 110016, PR China.
authors.forename: Ma
authors.initials: M
authors.lastname: Youlei
authors.forename: Zhang
authors.initials: Z
authors.lastname: Jinghai
authors.forename: Zhang
authors.initials: Z
authors.lastname: Yuntao
authors.forename: Lin
authors.initials: L
authors.lastname: Jiaoshu
authors.forename: Wang
authors.initials: W
authors.lastname: Tianyi
authors.forename: Wu
authors.initials: W
authors.lastname: Chunfu
authors.forename: Zhang
authors.initials: Z
authors.lastname: Rong
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry* / Q000276:immunology* / Q000302:isolation & purification; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D019476:Insect Proteins / Q000737:chemistry* / Q000276:immunology* / Q000302:isolation & purification; D007814:Larva / Q000737:chemistry; D008969:Molecular Sequence Data; D009036:Moths / Q000737:chemistry*; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D015398:Signal Transduction; D047071:beta-Glucans / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D000949:Histocompatibility Antigens Class II; D019476:Insect Proteins; C405332:beta-1,3-glucan recognition protein, insect; D047071:beta-Glucans; C033363:beta-1,3-glucan