title: Properties of osteoconductive biomaterials: calcium phosphate cement with different ratios of platelet-rich plasma as identifiers.
abstract: This study aims to evaluate further the performance of a platelet-rich plasma (PRP) additive incorporated with calcium phosphate bone cement (CPC) in vitro to prove its efficiency as bone graft substitutes and its compatibility to be incorporated into the CPC with other techniques in clinical restoration in vivo. The growth factor release ability and the osteogenic evaluation of PRP, CPC, and PRP/CPC testing groups with 5, 10, and 15 wt.% PRP were compared in vitro. Four groups were measured using non-decalcified staining methods in vivo, which include the testing group of 10 wt.% PRP/CPC selected from the evaluation in vitro, by using both the autograft with rabbit trabecular and CPC-only as comparison groups and the group without grafting material as the control sample. The results obtained through specimen immersion show that growth factor release and alkaline phosphatase activities after osteoprogenitor cell culture had a significantly better effect on 10 and 15 wt.% PRP/CPC than on the other groups in vitro. Analysis results suggest that PRP was still retained in the CPC matrix even after 32 days of immersion. The results in vivo show that the histology of the autograft bone and the control group without grafting material exhibited fibrous connective and adipose tissues, which obviously filled the created cavity even at nine weeks after the operation. Osteoregeneration was more successful in the PRP-additive group, which accumulated bone remodeling than in the other groups. In conclusion, CPC could be a potential carrier with adequate PRP additives that bear a therapeutic potential for enhanced bone tissue regeneration.
authors.affiliation: School of Dentistry, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Chia-Ling
authors.initials: CL
authors.lastname: Ko
authors.forename: Wen-Cheng
authors.initials: WC
authors.lastname: Chen
authors.forename: Jian-Chih
authors.initials: JC
authors.lastname: Chen
authors.forename: Ying-Hui
authors.initials: YH
authors.lastname: Wang
authors.forename: Chi-Jen
authors.initials: CJ
authors.lastname: Shih
authors.forename: Yu-Chang
authors.initials: YC
authors.lastname: Tyan
authors.forename: Chun-Cheng
authors.initials: CC
authors.lastname: Hung
authors.forename: Jen-Chyan
authors.initials: JC
authors.lastname: Wang
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D018786:Bone Substitutes / Q000737:chemistry* / Q000494:pharmacology / Q000627:therapeutic use; D016025:Bone Transplantation; D001842:Bone and Bones / Q000378:metabolism / Q000473:pathology; D002130:Calcium Phosphates / Q000737:chemistry*; D002460:Cell Line; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010982:Platelet-Derived Growth Factor / Q000378:metabolism; D053657:Platelet-Rich Plasma; D011817:Rabbits; D014182:Transplantation, Autologous
chemical_list: D018786:Bone Substitutes; D002130:Calcium Phosphates; D010982:Platelet-Derived Growth Factor; C020243:calcium phosphate; D000469:Alkaline Phosphatase