title: The effect of dietary protein levels on the expression of genes coding for four selected protein translation initiation factors in muscle tissue of Wujin pig.
abstract: The objective of this study was to investigate the regulatory mechanism underlying the increased muscle protein accumulation in pigs while were fed a high protein diet. The eukaryotic initiation factors (eIFs) have been reported to involve in muscle protein synthesis. We investigated the mRNA and protein expression levels of eIF2B1, 4A1, 4B and 4E in Wujin pigs fed either a high protein (HP: 18%) or a low protein (LP: 14%) diet at 30, 60 or 100 kg body weight, based on real-time PCR and western blotting analyses. Our results indicated that the expression levels of eIF2B1 mRNA and protein were increased by HP diet at all body weight. The HP diet showed higher mRNA and protein levels of eIF4B gene at 60 and 100 kg. The protein expression of eIF4E phosphorylation was increased by HP diet only at 30 kg. These data suggested that the HP diet promoted porcine muscle protein accumulation mainly by up-regulating eIF2B1, 4B and 4E rather than 4A1 expression along the growth stages.
authors.affiliation: College of Life Sciences, Yunnan Normal University, Kunming, Yunnan, China.
authors.forename: L Y
authors.initials: LY
authors.lastname: Liu
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.forename: Y
authors.initials: Y
authors.lastname: Huang
authors.forename: H B
authors.initials: HB
authors.lastname: Pan
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.forename: Z X
authors.initials: ZX
authors.lastname: Huang
authors.forename: S M
authors.initials: SM
authors.lastname: Zhao
authors.forename: S Z
authors.initials: SZ
authors.lastname: Gao
mesh_terms: D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D004032:Diet / Q000662:veterinary; D004044:Dietary Proteins / Q000494:pharmacology*; D039642:Eukaryotic Initiation Factors / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects*; D018482:Muscle, Skeletal / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013552:Swine / Q000235:genetics / Q000378:metabolism*
chemical_list: D004044:Dietary Proteins; D039642:Eukaryotic Initiation Factors; D012333:RNA, Messenger
keywords: dietary protein level; gene expression; swine; translation initiation factor