title: Crystallization and initial X-ray data of abscisic acid receptor PYL3 in the presence of (-)-ABA.
abstract: Abscisic acid (ABA) modulates many complicated developmental processes and responses to environmental stimuli. Recently, several (+)-ABA signalling mechanisms by the RCAR/PYR1/PYL family of proteins (PYLs) have been proposed. However, the mechanism of the recognition and binding of the unnatural ligand (-)-ABA by PYLs has not yet been elucidated. In the present study, the expression, purification and crystallization of PYL3 in complex with (-)-ABA are reported. Diffraction data were refined to 2.65 Å resolution for this complex in space group P65. These findings will help to explain the stereospecificity of PYLs for (-)-ABA and to explore the selective ABA agonists.
authors.affiliation: Clinical Medicine Research Center, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Guangdong 524001, PR China. cheungxingliang@gmail.com
authors.forename: Xingliang
authors.initials: X
authors.lastname: Zhang
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Wang
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D017360:Arabidopsis; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000378:metabolism*; D003460:Crystallization; D011956:Receptors, Cell Surface / Q000737:chemistry* / Q000378:metabolism*; D014961:X-Ray Diffraction
chemical_list: D029681:Arabidopsis Proteins; C572912:PYL3 protein, Arabidopsis; D011956:Receptors, Cell Surface; D000040:Abscisic Acid
keywords: (-)-ABA; PYL3; abscisic acid receptor