title: Use of Vitek 2 antimicrobial susceptibility profile to identify mecC in methicillin-resistant Staphylococcus aureus.
abstract: The emergence of mecC methicillin-resistant Staphylococcus aureus (MRSA) poses a diagnostic challenge for clinical microbiology laboratories. Using the Vitek 2 system, we tested a panel of 896 Staphylococcus aureus isolates and found that an oxacillin-sensitive/cefoxitin-resistant profile had a sensitivity of 88.7% and a specificity of 99.5% for the identification of mecC MRSA isolates. The presence of the mecC gene, determined by bacterial whole-genome sequencing, was used as the gold standard. This profile could provide a zero-cost screening method for identification of mecC-positive MRSA strains.
authors.affiliation: Department of Medicine, University of Cambridge, Cambridge, United Kingdom.
authors.forename: Edward J P
authors.initials: EJ
authors.lastname: Cartwright
authors.forename: Gavin K
authors.initials: GK
authors.lastname: Paterson
authors.forename: Kathy E
authors.initials: KE
authors.lastname: Raven
authors.forename: Ewan M
authors.initials: EM
authors.lastname: Harrison
authors.forename: Theodore
authors.initials: T
authors.lastname: Gouliouris
authors.forename: Angela
authors.initials: A
authors.lastname: Kearns
authors.forename: Bruno
authors.initials: B
authors.lastname: Pichon
authors.forename: Giles
authors.initials: G
authors.lastname: Edwards
authors.forename: Robert L
authors.initials: RL
authors.lastname: Skov
authors.forename: Anders R
authors.initials: AR
authors.lastname: Larsen
authors.forename: Mark A
authors.initials: MA
authors.lastname: Holmes
authors.forename: Julian
authors.initials: J
authors.lastname: Parkhill
authors.forename: Sharon J
authors.initials: SJ
authors.lastname: Peacock
authors.forename: M Estée
authors.initials: ME
authors.lastname: Török
mesh_terms: D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification* / Q000187:drug effects*; D008826:Microbial Sensitivity Tests / Q000379:methods; D012680:Sensitivity and Specificity