title: "Chemical-pain sensor" based on nanovesicle-carbon nanotube hybrid structures.
abstract: We developed a "chemical-pain sensor" that could recognize chemical pain stimuli such as capsaicin and resiniferatoxin just like mammalian chemical pain sensory systems. Here, we first prepared nanovesicles containing rat pain sensory receptor, rat transient receptor potential vanilloid 1 (rTRPV1), which is activated by noxious heat and capsaicin. And the nanovesicles were immobilized on a single-walled carbon nanotube-based field effect transistor. The chemical-pain sensor could selectively detect chemical pain stimuli with a high sensitivity of a 1 pM detection limit. It also responded to different chemical pain stimuli in a manner similar as to that of mammalian chemical pain sensory systems. This sensor platform can be utilized for various practical applications such as food screening tools and artificial somesthetic sensors. Moreover, TRP families have been suggested as potential drug targets related to nerve and circulation disorders. Thus, the capability of monitoring TRP responses using our sensor platforms should provide a powerful means for the development of new drugs as well as the basic research about nerve and circulation systems.
authors.affiliation: Department of Physics and Astronomy, Seoul National University, Seoul 151-747, Republic of Korea.
authors.forename: Hye Jun
authors.initials: HJ
authors.lastname: Jin
authors.forename: Jeong Mi
authors.initials: JM
authors.lastname: An
authors.forename: Juhun
authors.initials: J
authors.lastname: Park
authors.forename: Seok Jun
authors.initials: SJ
authors.lastname: Moon
authors.forename: Seunghun
authors.initials: S
authors.lastname: Hong
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation*; D002211:Capsaicin / Q000009:adverse effects; D003001:Cloning, Molecular; D004224:Diterpenes / Q000009:adverse effects; D057809:HEK293 Cells; D006358:Hot Temperature; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D057230:Limit of Detection; D049329:Nanostructures / Q000737:chemistry; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010146:Pain / Q000139:chemically induced* / Q000378:metabolism; D051381:Rats; D012680:Sensitivity and Specificity; D018689:Sensory System Agents / Q000009:adverse effects; D050916:TRPV Cation Channels / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D004224:Diterpenes; D055767:Immobilized Proteins; D037742:Nanotubes, Carbon; D018689:Sensory System Agents; D050916:TRPV Cation Channels; C493922:Trpv1 protein, rat; C024353:resiniferatoxin; D002211:Capsaicin
keywords: Carbon nanotube; Field effect transistor; Hybrid structure; Nanovesicle; Pain sensor