title: Detection of Vibrio cholerae using the intrinsic catalytic activity of a magnetic polymeric nanoparticle.
abstract: A novel and sensitive magnetic polymeric nanoparticle (MPNP)-polymerase chain reaction-colorimetry (magneto-PCR-colorimetry) technique was developed for detection of Vibrio cholerae ( V. cholerae ). The technique involved an amplification of V. cholerae DNA on the surface of an MPNP and then employed the intrinsic catalytic activity of the MPNP to detect the target gene by colorimetry. An amino-modified forward primer was covalently labeled onto the MPNP surface which would bind to PCR product during PCR cycling. By employing the catalytic activity of the MPNP, the analysis of PCR product bound MPNP yielded a sensitivity of 10(3) CFU/mL of V. cholerae in buffer system within 4 h. The specificity and efficiency of the technique were investigated by using various bacterial DNAs in drinking and tap water.
authors.affiliation: National Nanotechnology Center, National Science and Technology Development Agency, Thailand Science Park, Pathum Thani 12120, Thailand.
authors.forename: Raweewan
authors.initials: R
authors.lastname: Thiramanas
authors.forename: Kulachart
authors.initials: K
authors.lastname: Jangpatarapongsa
authors.forename: Pramuan
authors.initials: P
authors.lastname: Tangboriboonrat
authors.forename: Duangporn
authors.initials: D
authors.lastname: Polpanich
mesh_terms: D002384:Catalysis; D003124:Colorimetry / Q000379:methods; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011108:Polymers / Q000737:chemistry*; D014734:Vibrio cholerae / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D058185:Magnetite Nanoparticles; D011108:Polymers