title: Crystallization and X-ray diffraction analysis of an antifungal laticifer protein.
abstract: An osmotin (CpOsm) from the latex of Calotropis procera has been crystallized in both tetragonal and trigonal forms suitable for structure determination. Crystallographic studies of CpOsm are of great interest because limited information is available concerning the structure of latex proteins and CpOsm has previously been shown to interact with the spore membranes of some plant pathogenic fungi, thus impairing spore germination and hyphal growth. CpOsm crystals were grown using 0.1 M HEPES buffer pH 7.5, 26% PEG 4000, 0.2 M ammonium sulfate (space group P4(3)) or using 0.1 M HEPES buffer pH 7.5, 35% MPD, 0.7 M ammonium sulfate (space group P3(1)12). X-ray diffraction data were collected to 2.17 Å (P4(3)) and 1.80 Å (P3(1)12) resolution and molecular-replacement analyses produced initial phases for both crystal forms.
authors.affiliation: Centro de Ciências da Saúde, Universidade de Fortaleza, Avenida Washington Soares 1321, Bairro Edson Queiroz, 60811-905 Fortaleza-CE, Brazil.
authors.forename: Frederico
authors.initials: F
authors.lastname: Bruno-Moreno
authors.forename: Raquel
authors.initials: R
authors.lastname: Sombra Basílio de Oliveira
authors.forename: Renato
authors.initials: R
authors.lastname: de Azevedo Moreira
authors.forename: Marina Duarte
authors.initials: MD
authors.lastname: Pinto Lobo
authors.forename: Cléverson Diniz
authors.initials: CD
authors.lastname: Teixeira de Freitas
authors.forename: Márcio
authors.initials: M
authors.lastname: Viana Ramos
authors.forename: Thalles
authors.initials: T
authors.lastname: Barbosa Grangeiro
authors.forename: Ana Cristina
authors.initials: AC
authors.lastname: Oliveira Monteiro-Moreira
mesh_terms: D000935:Antifungal Agents / Q000737:chemistry* / Q000302:isolation & purification; D031107:Calotropis; D003460:Crystallization; D007840:Latex / Q000737:chemistry* / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification; D021241:Spectrometry, Mass, Electrospray Ionization; D014961:X-Ray Diffraction
chemical_list: D000935:Antifungal Agents; D007840:Latex; D010936:Plant Extracts; D010940:Plant Proteins
keywords: Calotropis procera; PR-5; latex proteins; osmotins; thaumatin-like proteins