title: Protection against avian necrotic enteritis after immunisation with NetB genetic or formaldehyde toxoids.
abstract: NetB (necrotic enteritis toxin B) is a recently identified β-pore-forming toxin produced by Clostridium perfringens. This toxin has been shown to play a major role in avian necrotic enteritis. In recent years, a dramatic increase in necrotic enteritis has been observed, especially in countries where the use of antimicrobial growth promoters in animal feedstuffs has been banned. The aim of this work was to determine whether immunisation with a NetB toxoid would provide protection against necrotic enteritis. The immunisation of poultry with a formaldehyde NetB toxoid or with a NetB genetic toxoid (W262A) resulted in the induction of antibody responses against NetB and provided partial protection against disease.
authors.affiliation: College of Life and Environmental Sciences, University of Exeter, Stocker Road, Exeter EX4 4QD, United Kingdom.
authors.forename: Sérgio P
authors.initials: SP
authors.lastname: Fernandes da Costa
authors.forename: Dorien
authors.initials: D
authors.lastname: Mot
authors.forename: Monika
authors.initials: M
authors.lastname: Bokori-Brown
authors.forename: Christos G
authors.initials: CG
authors.lastname: Savva
authors.forename: Ajit K
authors.initials: AK
authors.lastname: Basak
authors.forename: Filip
authors.initials: F
authors.lastname: Van Immerseel
authors.forename: Richard W
authors.initials: RW
authors.lastname: Titball
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis; D001427:Bacterial Toxins / Q000235:genetics / Q000276:immunology*; D002645:Chickens / Q000276:immunology / Q000382:microbiology; D003015:Clostridium Infections / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D003016:Clostridium perfringens / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D004751:Enteritis / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay; D005557:Formaldehyde / Q000276:immunology; D007114:Immunization / Q000379:methods; D009154:Mutation; D011201:Poultry Diseases / Q000382:microbiology; D014121:Toxoids / Q000276:immunology / Q000494:pharmacology*
chemical_list: D000907:Antibodies, Bacterial; D001427:Bacterial Toxins; C572202:NE B-like toxin, Clostridium perfringens; D014121:Toxoids; D005557:Formaldehyde
keywords: Chicken; Clostridium perfringens; Necrotic enteritis; NetB; Pore-forming toxin; Vaccine