title: Analysis of acyclovir in vitreous humor by a validated HPLC method.
abstract: An HPLC-UV method was developed and validated for the determination of acyclovir in vitreous humor. The method was carried out in isocratic mode using 0.02 mol/L acetic acid/methanol (95:5) as mobile phase, a C18 column at 25 degrees C and UV detection at 254 nm. The method was linear (r2> 0.99) over the range of 35-700 microg/mL, precise (RSD <5%), accurate (recovery ranged from 98.18 to 99.64%), robust, selective regarding of the vitreous humor, and robust remaining unaffected by deliberate variations in relevant parameters. The validated HPLC-UV method can be successfully applied to determine acyclovir directly injected into the vitreous cavity of rabbits' eye.
authors.affiliation: School of Pharmacy, Federal University of São João Del, Divinópolis, Brazil. silvia.fialho@funed.mg.gov.br
authors.forename: G R
authors.initials: GR
authors.lastname: Silva
authors.forename: A S P
authors.initials: AS
authors.lastname: Caldeira
authors.forename: F M
authors.initials: FM
authors.lastname: Damico
authors.forename: B S
authors.initials: BS
authors.lastname: Takahashi
authors.forename: A
authors.initials: A
authors.lastname: Silva-Cunha
authors.forename: S L
authors.initials: SL
authors.lastname: Fialho
mesh_terms: D000212:Acyclovir / Q000032:analysis*; D000818:Animals; D000998:Antiviral Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D007202:Indicators and Reagents; D057230:Limit of Detection; D011817:Rabbits; D012015:Reference Standards; D015203:Reproducibility of Results; D015882:Retinal Necrosis Syndrome, Acute / Q000188:drug therapy; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet; D014822:Vitreous Body / Q000737:chemistry*
chemical_list: D000998:Antiviral Agents; D007202:Indicators and Reagents; D012996:Solutions; D000212:Acyclovir