title: [Experimental study on polysaccharide of Cipangopaludina chinensis against HBV in vitro].
abstract: OBJECTIVE
  To evaluate the biological activity of polysaccharide of Cipangopaludina chinensis (PCC) against HBV in vitro.
  
  
  METHODS
  HepG2 2. 2. 15 cells were taken as the in vitro experimental model. The cell toxicity was observed by MTT. PCC of different safe concentrations and positive control medicine 3TC were added into the cells. Cell control without medicine was set at the same time. Cultural supernatants were collected at 9 d. HBsAg and HBeAg in cultural supernatants were tested by ELISA. The content of HBV-DNA was detected by TaqMan probe fluorescence quantitative PCR.
  
  
  RESULTS
  TC0 and TC50 of PCC in HepG2 2. 2. 15 cell culture were 1 g . L-1 and >10 g . L-1, respectively, suggesting low toxicity in cells. IC50 of PCC in HepG2 2. 2. 15 cells HBsAg and HBeAg were 0. 501, 0. 401 g. L-1, with SI being >19.96 and >24. 94, respectively. PCC could effectively inhibit the secretion of HBsAg and HBeAg, and have a better effect on HBeAg than on HBsAg. PCC had a significant inhibitory effect on HBV-DNA in HepG2 2. 2. 15 cells at concentrations of 0. 1, 1 g . L-1 P <0.05).
  
  
  CONCLUSIONS
  PCC has the effect against HBV activity in vitro to some extent, with low toxicity, thereby having a good prospect for application.
authors.affiliation: School of Medicine, Anhui University of Science & Technology, Huainan 232001, China.
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Liu
authors.forename: Chao-Pin
authors.initials: CP
authors.lastname: Li
authors.forename: Ke-Xia
authors.initials: KX
authors.lastname: Wang
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000009:adverse effects / Q000494:pharmacology*; D004279:DNA, Viral / Q000378:metabolism; D056945:Hep G2 Cells; D006514:Hepatitis B Surface Antigens / Q000378:metabolism; D006513:Hepatitis B e Antigens / Q000378:metabolism; D006515:Hepatitis B virus / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D011134:Polysaccharides / Q000009:adverse effects / Q000494:pharmacology*; D012908:Snails / Q000737:chemistry*
chemical_list: D000998:Antiviral Agents; D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D011134:Polysaccharides