title: Summer heat stress affects prostaglandin synthesis in the bovine oviduct.
abstract: Summer heat stress (HS) negatively affects reproductive functions, including prostaglandin (PG) F2α secretion in the endometrium, and decreases fertility in cattle. In the present study, we examined the effects of elevated temperatures on PG synthesis in oviductal epithelial cells. The epithelial cells obtained from the ampulla and isthmus of the oviduct were incubated at various temperatures (38.5, 39.5, 40.0, and 40.5 °C) for 24 h. In the ampulla, PGE₂ concentration was higher at 40.5 °C than at 38.5 °C, while PGF2α production was not affected by the temperatures in this range. The expressions of microsomal PGE synthase 1 (PTGES (mPGES1)), cytosolic PGES (PTGES3 (cPGES)), and heat shock protein 90 (HSP90AA1 (HSP90)) mRNAs and proteins were higher at 40.5 °C than at 38.5 °C in the ampullary epithelial cells. Seasonal changes in the expressions of PGES and HSP90AA1 mRNAs in oviductal tissues were also investigated. The expressions of PTGES3 and HSP90AA1 mRNAs were higher in the ampullary tissues in summer than in winter. In summary, elevated temperatures stimulated PGE₂ production in the ampullary oviduct by increasing the expressions of PGESs and HSP90AA1, which can activate cPGES. The overall results suggest that HS upsets PG secretions and reduces oviductal smooth muscle motility, which in turn could decrease gamete/embryo transport through the oviduct.
authors.affiliation: Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University, 1-1-1 Tsushimanaka, Okayama, Okayama 700-8530, Japan.
authors.forename: Yoshihiko
authors.initials: Y
authors.lastname: Kobayashi
authors.forename: Kaori
authors.initials: K
authors.lastname: Wakamiya
authors.forename: Misa
authors.initials: M
authors.lastname: Kohka
authors.forename: Yuki
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Okuda
mesh_terms: D000003:Abattoirs; D000818:Animals; D017849:Animals, Inbred Strains; D002417:Cattle; D002418:Cattle Diseases / Q000378:metabolism* / Q000473:pathology; D002478:Cells, Cultured; D003600:Cytosol / Q000201:enzymology; D015237:Dinoprost / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D004790:Enzyme Induction; D005260:Female; D018841:HSP90 Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D018882:Heat Stress Disorders / Q000378:metabolism / Q000473:pathology / Q000662:veterinary*; D006358:Hot Temperature / Q000009:adverse effects; D019746:Intramolecular Oxidoreductases / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D007564:Japan; D008861:Microsomes / Q000201:enzymology; D010057:Oviducts / Q000166:cytology / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D000071876:Prostaglandin-E Synthases; D011453:Prostaglandins / Q000378:metabolism*; D012621:Seasons
chemical_list: D018841:HSP90 Heat-Shock Proteins; D011453:Prostaglandins; D015237:Dinoprost; D019746:Intramolecular Oxidoreductases; D000071876:Prostaglandin-E Synthases; D015232:Dinoprostone