title: Structural variation and dynamics of the nuclear ribosomal intergenic spacer region in key members of the Gibberella fujikuroi species complex.
abstract: The intergenic spacer (IGS) region of the ribosomal DNA was cloned and sequenced in eight species within the Gibberella fujikuroi species complex with anamorphs in the genus Fusarium, a group that includes the most relevant toxigenic species. DNA sequence analyses revealed two categories of repeated elements: long repeats and short repeats of 125 and 8 bp, respectively. Long repeats were present in two copies and were conserved in all the species analyzed, whereas different numbers of short repeat elements were observed, leading to species-specific IGS sequences with different length. In Fusarium subglutinans and Fusarium nygamai, these differences seemed to be the result of duplication and deletion events. Here, we propose a model based on unequal crossing over that can explain these processes. The partial IGS sequence of 22 Fusarium proliferatum isolates was also obtained to study variation at the intraspecific level. The results revealed no differences in terms of number or pattern of repeated elements and detected frequent gene conversion events. These results suggest that the homogenization observed at the intraspecific level might not be achieved primarily by unequal crossing-over events but rather by processes associated with recombination such as gene conversion events.
authors.affiliation: Department of Molecular Evolution, Centro de Astrobiología, CSIC-INTA, Torrejón de Ardoz, 28850 Madrid, Spain. miretecs@cab.inta-csic.es
authors.forename: Salvador
authors.initials: S
authors.lastname: Mirete
authors.forename: Belén
authors.initials: B
authors.lastname: Patiño
authors.forename: Miguel
authors.initials: M
authors.lastname: Jurado
authors.forename: Covadonga
authors.initials: C
authors.lastname: Vázquez
authors.forename: María Teresa
authors.initials: MT
authors.lastname: González-Jaén
mesh_terms: D003434:Crossing Over, Genetic; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D005670:Fusarium / Q000235:genetics; D005874:Gibberella / Q000235:genetics*; D010802:Phylogeny; D012091:Repetitive Sequences, Nucleic Acid
chemical_list: D021903:DNA, Ribosomal Spacer