title: SPE and purification of DNA using magnetic particles.
abstract: Superparamagnetic particles have been attractive for molecular diagnostics and analytical chemistry applications due to their unique magnetic properties and their ability to interact with various biomolecules of interest. This paper presents a critical overview of magnetic nano- and microparticles used as a solid phase for extraction and purification of DNAs. The mechanisms of DNA binding to the surface of functionalised magnetic particles are described. The most widely used materials including silica supports, organic polymers and other materials, mostly containing magnetite or paramagnetic metallic elements are reviewed. The main application areas of magnetic particles for DNA separation are briefly described.
authors.affiliation: Faculty of Chemistry, Brno University of Technology, Brno, Czech Republic. rittich@fch.vutbr.cz
authors.forename: Bohuslav
authors.initials: B
authors.lastname: Rittich
authors.forename: Alena
authors.initials: A
authors.lastname: Spanová
mesh_terms: D004247:DNA / Q000302:isolation & purification*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000295:instrumentation
chemical_list: D058185:Magnetite Nanoparticles; D004247:DNA
keywords: Core covering; DNA isolation; DNA pre-concentration; Magnetic particles; Mechanisms