title: Structural and water-holding characteristics of untreated and ensiled chicory root pulp.
abstract: Cell wall polysaccharides (CWPs) from chicory root pulp (CRP) and the effect of ensiling on CWP structure to reduce the water-holding capacity (WHC) were studied. Sequential extractions of CRP showed that hot water extraction solubilized arabinan-rich pectin and inulin, each representing 6% of all CRP sugars. A significant amount of pectic sugars (46%) rich in uronic acid from CRP was solubilized by chelating agents. Both dilute alkali extraction, which solubilized branched pectin (14% from CRP), and concentrated alkali extraction, which solubilized hemicellulose dominant in xyloglucans (2.5%) mostly of the XXXG type and mannan (0.9%), from CRP CWPs seemed to influence the WHC of CRP. Alkali-insoluble residue (39% of CRP sugars) mainly comprised cellulose and some branched pectin (17% from CRP). Ensiling reduced the methyl esterification of pectin, caused degradation of homogalacturonan and rhamnogalacturonan, and possibly modified the xyloglucan, mannan, and glucan network, reducing the WHC from 6 mL/g to 3.4 mL/g.
authors.affiliation: Laboratory of Food Chemistry, Wageningen University , P.O. Box 8129, 6700 EV Wageningen, The Netherlands.
authors.forename: Uttara S
authors.initials: US
authors.lastname: Ramasamy
authors.forename: Harry
authors.initials: H
authors.lastname: Gruppen
authors.forename: Henk A
authors.initials: HA
authors.lastname: Schols
mesh_terms: D002473:Cell Wall / Q000737:chemistry; D018651:Cichorium intybus / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D014867:Water / Q000032:analysis
chemical_list: D010936:Plant Extracts; D011134:Polysaccharides; D014867:Water