title: Two-dimensional microscale engineering of protein-based nanoparticles for cell guidance.
abstract: Cell responses, such as positioning, morphological changes, proliferation, and apoptosis, are the result of complex chemical, topographical, and biological stimuli. Here we show the macroscopic responses of cells when nanoscale profiles made with inclusion bodies (IBs) are used for the 2D engineering of biological interfaces at the microscale. A deep statistical data treatment of fibroblasts cultivated on supports patterned with green fluorescent protein and human basic fibroblast growth factor-derived IBs demonstrates that these cells preferentially adhere to the IB areas and align and elongate according to specific patterns. These findings prove the potential of surface patterning with functional IBs as protein-based nanomaterials for tissue engineering.
authors.affiliation: Department of Molecular Nanoscience and Organic Materials, Institut de Ciencia de Materials de Barcelona (CSIC), Bellaterra, 08193 Barcelona, Spain.
authors.forename: Witold I
authors.initials: WI
authors.lastname: Tatkiewicz
authors.forename: Joaquin
authors.initials: J
authors.lastname: Seras-Franzoso
authors.forename: Elena
authors.initials: E
authors.lastname: García-Fruitós
authors.forename: Esther
authors.initials: E
authors.lastname: Vazquez
authors.forename: Nora
authors.initials: N
authors.lastname: Ventosa
authors.forename: Karl
authors.initials: K
authors.lastname: Peebo
authors.forename: Imma
authors.initials: I
authors.lastname: Ratera
authors.forename: Antonio
authors.initials: A
authors.lastname: Villaverde
authors.forename: Jaume
authors.initials: J
authors.lastname: Veciana
mesh_terms: D000818:Animals; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D002479:Inclusion Bodies / Q000378:metabolism; D051379:Mice; D055616:Microtechnology / Q000379:methods*; D041681:NIH 3T3 Cells; D053758:Nanoparticles / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D011506:Proteins