title: Melatonin ameliorates oxidative stress and reproductive toxicity induced by cyclophosphamide in male mice.
abstract: The present study evaluated the efficacy of melatonin in cyclophosphamide (CP)-induced testicular injury, lipid peroxidative damage, and antioxidant enzymes status of the mice testis on the basis of biochemical and histological studies. Mice were pretreated with four different doses of melatonin (2.5, 5, 10, and, 20 mg/kg by body weight (b.w.)) via intraperitoneal injection for five consecutive days followed by injection with CP (200 mg/kg b.w.) 1 h after the last injection of melatonin on the 5th day. After 24 h, mice were euthanized, testes were immediately removed, and biochemical and histological studies were conducted. Treatment with melatonin significantly mitigates lipid peroxidation, superoxide dismutase, and catalase activity and the level of reduced glutathione content abnormality induced by CP in mice testis. Histological examination clearly demonstrates that pretreatment of melatonin prevented CP-induced spermatogenesis toxicity and spermatogenic cells reduction in mice testis. The protective effect of melatonin is likely due to the antioxidative properties of the indolamine existed in the chemical structure. Because melatonin is a safe, natural compound, it could be used concomitantly as a supplement to protect people undergoing chemotherapy against reproductive toxicity.
authors.affiliation: 1Student Research Committee, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Islamic Republic of Iran.
authors.forename: A
authors.initials: A
authors.lastname: Chabra
authors.forename: M
authors.initials: M
authors.lastname: Shokrzadeh
authors.forename: F
authors.initials: F
authors.lastname: Naghshvar
authors.forename: F
authors.initials: F
authors.lastname: Salehi
authors.forename: A
authors.initials: A
authors.lastname: Ahmadi
mesh_terms: D000818:Animals; D018906:Antineoplastic Agents, Alkylating / Q000633:toxicity*; D000975:Antioxidants / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology; D003520:Cyclophosphamide / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D007248:Infertility, Male / Q000139:chemically induced* / Q000188:drug therapy; D015227:Lipid Peroxidation; D008297:Male; D008550:Melatonin / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D051379:Mice; D015394:Molecular Structure; D018384:Oxidative Stress / Q000187:drug effects*
chemical_list: D018906:Antineoplastic Agents, Alkylating; D000975:Antioxidants; D003520:Cyclophosphamide; D008550:Melatonin
keywords: Melatonin; cyclophosphamide; oxidative stress; reproductive toxicity; testicular toxicity