title: Downregulation of KLF8 expression by shRNA induces inhibition of cell proliferation in CAL27 human oral cancer cells.
abstract: OBJECTIVE
  KLF8 is a member of KLF transcription factors which play an important tolr in oncogenesis. It is barely expressed in normal human epithelial cells but highly overexpressed in several types of human cancer cell lines. In the present study, we investigate the role of KLF8 in oral cancer and the effects of KLF8 knockdown via lentivirus mediated siRNA infection in human adenosquamos carcinoma CAL 27 cells.
  
  
  METHODS
  We developed a vector-based siRNA expression system that can induce RNAi in CAL 27 oral cancer cells. Downregulation of KLF8 was confirmed by evaluating GFP expressions, RT-PCR and western blot analysis. Finally, the effects of KLF8 downregulation were analyzed by MTT assay and colony formation assays.
  
  
  RESULTS
  The expression levels of KLF8 mRNA and proteins are reduced in CAL 27 cells that transfected with 21-nt siRNA against KLF8. Lentivirus-mediated silencing of KLF8 reduces cell proliferation and colonies number, thereby indicating the role of KLF8 in cell proliferation and tumorigenesis.
  
  
  CONCLUSIONS
  These results strongly suggest that KLF8 is essential for growth of CAL 27 cancer cells. A better understanding of KLF8 function and processing may provide novel insights into the clinical therapy of oral cancer.
authors.affiliation: Department of Anatomy, Shandong University School of Medicine, Jinan 250012, Shandong Province, PR China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Bin
authors.forename: Li
authors.initials: L
authors.lastname: Ke-Yi
authors.forename: Zhang
authors.initials: Z
authors.lastname: Wei-Feng
authors.forename: Jiang
authors.initials: J
authors.lastname: Li-Cheng
authors.forename: Liu
authors.initials: L
authors.lastname: Xian-Bin
authors.forename: Xia
authors.initials: X
authors.lastname: Chun-Peng
authors.forename: Yuan
authors.initials: Y
authors.lastname: Dao-Ying
authors.forename: Liu
authors.initials: L
authors.lastname: Shu-Wei
mesh_terms: D018196:Carcinoma, Adenosquamous / Q000378:metabolism* / Q000473:pathology* / Q000821:virology; D049109:Cell Proliferation; D015536:Down-Regulation; D006801:Humans; D051741:Kruppel-Like Transcription Factors; D016180:Lentivirus Infections; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000473:pathology* / Q000821:virology; D034741:RNA, Small Interfering / Q000502:physiology*; D012097:Repressor Proteins / Q000096:biosynthesis*; D014407:Tumor Cells, Cultured
chemical_list: C507402:KLF8 protein, human; D051741:Kruppel-Like Transcription Factors; D034741:RNA, Small Interfering; D012097:Repressor Proteins