title: Upregulation of miR-370 contributes to the progression of gastric carcinoma via suppression of FOXO1.
abstract: FOXO1 is downregulated in a number of cancers. However, the underlying mechanisms are poorly understood. In this study, we report that the expression of miR-370 was upregulated in gastric cancer cell lines and gastric cancer tissues. Overexpression of miR-370 in gastric cancer cells promoted the cell proliferation and anchorage-independent growth, while silencing of miR-370 showed opposite effects. miR-370-induced proliferation was correlated with the downregulation of cyclin-dependent kinase inhibitors, p27(Kip1) and p21(Cip1), and the upregulation of the cell cycle regulator cyclin D1. Furthermore, we identified that FOXO1 is the functional target of miR-370. Restored expression of FOXO1 together with miR-370 strongly abrogated miR-370-induced cell proliferation. Taken together, our results revealed a novel mechanism of FOXO1 suppression mediated by miR-370 in gastric cancer.
authors.affiliation: XuZhou Children's Hospital, 18 Sudi North Road, Xuzhou, Jiangsu Province 221006, PR China.
authors.forename: Conghai
authors.initials: C
authors.lastname: Fan
authors.forename: Sheng
authors.initials: S
authors.lastname: Liu
authors.forename: Yupeng
authors.initials: Y
authors.lastname: Zhao
authors.forename: Youqun
authors.initials: Y
authors.lastname: Han
authors.forename: Longqiu
authors.initials: L
authors.lastname: Yang
authors.forename: Guorong
authors.initials: G
authors.lastname: Tao
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
mesh_terms: D018797:Cell Cycle Proteins / Q000235:genetics; D048708:Cell Growth Processes / Q000235:genetics; D045744:Cell Line, Tumor; D018450:Disease Progression; D015536:Down-Regulation; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors / Q000235:genetics*; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology*; D015854:Up-Regulation
chemical_list: D018797:Cell Cycle Proteins; C085126:FOXO1 protein, human; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors; C526503:MIRN370 microRNA, human; D035683:MicroRNAs
keywords: Cycle progression; FOXO1; Gastric cancer; MicroRNA370; Proliferation