title: Use of hypertonic solutions for liver preservation in rats.
abstract: BACKGROUND
  The success of a transplant depends mainly on the viability of the graft, which is currently the main point of difficulty focuses on the triad preservation-rejection-infection. There are several specific components of preservation solutions that could prevent certain tissue damage. From these components, the osmotic factor has been highlighted as a factor in preventing edema and subsequent cell death, suggesting a possible advantage in the use of hypertonic solutions for organ preservation.
  
  
  OBJECTIVE
  To compare different hypertonic solutions as alternative to liver preservation.
  
  
  METHODS
  A total of 105 Wistar rats were divided in Standard Group (GP, n=5 rats), to verify the normal range of the study, and five experimental groups of 20 rats each, according to the preservation solution used: Group Eurocollins (GE), Group Saline 0.9% (GF), Group Glucose 50% (GG), Group Mannitol 20% (GM), Group Salty - NaCl 7.5% (GS). All animals in experimental group were also divided into four subgroups according to the time of collection in: 0 h, 2 h, 6 h and 12 h. Was assessed cell viability by the reaction with Methyl Blue Thiazolyl (MTT) and the dosages of lactate and alanine aminotransferase (ALT).
  
  
  RESULTS
  Regarding the lactate level, was observed a relative improvement of hypertonic solutions compared to eurocollins, and in 12 h, the GE and GS showed no statistically significant difference (p> 0.05). When assessed cell viability, absorbance at MTT also demonstrated favorable results to the GS, since no statistically significant difference in relation to GE.
  
  
  CONCLUSIONS
  The 7.5% NaCl solution showed promising results for organ preservation, presenting parameters and capability comparable to eurocollins preservation solution.
authors.affiliation: Experimental Surgery Laboratory of the Medical School, State University of Pará - UEPA, Belém, PA, Brazil. vitoryamaki@gmail.com
authors.forename: Vitor Nagai
authors.initials: VN
authors.lastname: Yamaki
authors.forename: Renan Kleber Costa
authors.initials: RK
authors.lastname: Teixeira
authors.forename: Marcus Vinicius Henriques
authors.initials: MV
authors.lastname: Brito
mesh_terms: D000818:Animals; D006982:Hypertonic Solutions; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D051381:Rats; D017208:Rats, Wistar
chemical_list: D006982:Hypertonic Solutions; D019188:Organ Preservation Solutions