title: Biotransformation of Ginsenoside Rb1 to Prosapogenins, Gypenoside XVII, Ginsenoside Rd, Ginsenoside F2, and Compound K by Leuconostoc mesenteroides DC102.
abstract: Ginsenoside Rb1is the main component in ginsenosides. It is a protopanaxadiol-type ginsenoside that has a dammarane-type triterpenoid as an aglycone. In this study, ginsenoside Rb1 was transformed into gypenoside XVII, ginsenoside Rd, ginsenoside F2 and compound K by glycosidase from Leuconostoc mesenteroides DC102. The optimum time for the conversion was about 72 h at a constant pH of 6.0 to 8.0 and the optimum temperature was about 30℃. Under optimal conditions, ginsenoside Rb1 was decomposed and converted into compound K by 72 h post-reaction (99%). The enzymatic reaction was analyzed by highperformance liquid chromatography, suggesting the transformation pathway: ginsenoside Rb1→ gypenoside XVII and ginsenoside Rd→ginsenoside F2→compound K.
authors.affiliation: Korean Ginseng Center Most Valuable Product and Ginseng Genetic Resource Bank, Kyung Hee University, Yongin 446-701, Korea.
authors.forename: Lin-Hu
authors.initials: LH
authors.lastname: Quan
authors.forename: Jin-Ying
authors.initials: JY
authors.lastname: Piao
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Min
authors.forename: Ho-Bin
authors.initials: HB
authors.lastname: Kim
authors.forename: Sang-Rae
authors.initials: SR
authors.lastname: Kim
authors.forename: Dong-Uk
authors.initials: DU
authors.lastname: Yang
authors.forename: Deok Chun
authors.initials: DC
authors.lastname: Yang
keywords: Biotransformation; Compound K; Ginsenoside Rd; Gypenoside XVII; Leuconostoc mesenteroides DC102