title: Atypical features of a Ure2p glutathione transferase from Phanerochaete chrysosporium.
abstract: Glutathione transferases (GSTs) are known to transfer glutathione onto small hydrophobic molecules in detoxification reactions. The GST Ure2pB1 from Phanerochaete chrysosporium exhibits atypical features, i.e. the presence of two glutathione binding sites and a high affinity towards oxidized glutathione. Moreover, PcUre2pB1 is able to efficiently deglutathionylate GS-phenacylacetophenone. Catalysis is not mediated by the cysteines of the protein but rather by the one of glutathione and an asparagine residue plays a key role in glutathione stabilization. Interestingly PcUre2pB1 interacts in vitro with a GST of the omega class. These properties are discussed in the physiological context of wood degrading fungi.
authors.affiliation: Université de Lorraine, IAM, UMR 1136, IFR 110 EFABA,Vandoeuvre-lès-Nancy F-54506, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Thuillier
authors.forename: Thomas
authors.initials: T
authors.lastname: Roret
authors.forename: Frédérique
authors.initials: F
authors.lastname: Favier
authors.forename: Eric
authors.initials: E
authors.lastname: Gelhaye
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Jacquot
authors.forename: Claude
authors.initials: C
authors.lastname: Didierjean
authors.forename: Mélanie
authors.initials: M
authors.lastname: Morel-Rouhier
mesh_terms: D018360:Crystallography, X-Ray; D004229:Dithiothreitol / Q000737:chemistry; D005656:Fungal Proteins / Q000737:chemistry*; D005978:Glutathione / Q000737:chemistry; D005982:Glutathione Transferase / Q000737:chemistry*; D006860:Hydrogen Bonding; D007328:Insulin / Q000737:chemistry; D007700:Kinetics; D008958:Models, Molecular; D010084:Oxidation-Reduction; D020075:Phanerochaete / Q000201:enzymology*; D011485:Protein Binding; D017433:Protein Structure, Secondary; D019163:Reducing Agents / Q000737:chemistry
chemical_list: D005656:Fungal Proteins; D007328:Insulin; D019163:Reducing Agents; D005982:Glutathione Transferase; D005978:Glutathione; D004229:Dithiothreitol