title: [Expression of astrocyte elevated gene-1 protein and its clinical significance in laryngeal squamous cell carcinoma].
abstract: OBJECTIVE
  To assess the protein expression of astrocyte elevated gene-1 (AEG-1) in tissue specimens of laryngeal squamous cell carcinoma (LSCC), and to correlate its expression with clinicopathological parameters and prognosis in patients with LSCC.
  
  
  METHODS
  RT-PCR was used to assay the expression of AEG-1 mRNA in 13 pairs of LSCC tissues and their corresponding noncarcinoma epithelia. Immunohistochemistry was performed on paraffin-embedded tissue specimens to investigate the protein expression of AEG-1 in 88 cases of LSCC specimens and 15 cases of adjacent epithelial samples.
  
  
  RESULTS
  The expression of AEG-1 mRNA was significantly increased in LSCC tissues compared to adjacent noncarcinoma epithelial tissues (0.81 ± 0.17 vs. 0.23 ± 0.10;t = 10.337, P < 0.001). Meantime, the positive rate of AEG-1 protein in 88 cases of LSCC was 87.5% (77/88). However, 15 cases of adjacent noncarcinoma epithelial merely demonstrated negative or mild expression of AEG-1 protein. AEG-1 overexpression was closely correlated with T stage (χ(2) = 6.289, P = 0.018), clinical stage (χ(2) = 11.049, P < 0.01), metastasis (χ(2) = 20.859, P < 0.01) and recurrence(χ(2) = 13.459, P < 0.01). The overall survival rates of patients with AEG-1 overexpression and low expression were 35.9% and 86.4%, respectively (χ(2) = 23.409, P < 0.01). Multivariate Cox regression analysis revealed that AEG-1 expression was an independent prognostic factor (P = 0.016).
  
  
  CONCLUSIONS
  AEG-1 protein may play a critical role in the initiation and progression of LSCC, implicating its predictive value in prognosis.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Central South University, Changsha, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Guo
authors.initials: G
authors.lastname: Li
authors.forename: Zhong-wu
authors.initials: ZW
authors.lastname: Su
authors.forename: Yuan-zheng
authors.initials: YZ
authors.lastname: Qiu
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Chang-yun
authors.initials: CY
authors.lastname: Yu
authors.forename: Xiao-juan
authors.initials: XJ
authors.lastname: Zhou
authors.forename: Shu-ling
authors.initials: SL
authors.lastname: Ren
authors.forename: Dong-hai
authors.initials: DH
authors.lastname: Huang
authors.forename: Yong-quan
authors.initials: YQ
authors.lastname: Tian
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology* / Q000601:surgery; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology* / Q000601:surgery; D008207:Lymphatic Metastasis; D008297:Male; D008565:Membrane Proteins; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local; D009367:Neoplasm Staging; D016016:Proportional Hazards Models; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins; D015996:Survival Rate
chemical_list: D015815:Cell Adhesion Molecules; C483039:MTDH protein, human; D008565:Membrane Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins