title: Efficient purification of high-purity compounds from the stem of Lonicera japonica Thunb using two-dimensional preparative chromatography.
abstract: Purification of high-purity compounds from traditional Chinese medicines (TCMs) plays an important role in investigating their bioactivity. Nevertheless, it is often quite difficult to isolate compounds with high purity because of the complexity of TCMs in chemical composition. In this work, a two-dimensional preparation method was successfully developed for the preparation of high-purity compounds from the stem of Lonicera japonica Thunb, based on two novel polar copolymerized RP stationary phases, XAqua C3 and XAqua C18. An XAqua C3 prep column was used to separate the sample in the first-dimensional preparation, and 14 g of sample was fractionated into eight fractions with a recovery of 82%. An XAqua C18 prep column was selected to prepare high-purity compounds in the second-dimensional preparation for its good orthogonality with the XAqua C3 stationary phase. As a result, major compounds in the sample were isolated with more than 99% purity. This method is a potent method to realize the efficient purification of compounds with high purity from the stem of L. japonica Thunb and it shows great potential in the separation of high-purity compounds from complex samples.
authors.affiliation: Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Jin
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Liu
authors.forename: Jiatao
authors.initials: J
authors.lastname: Feng
authors.forename: Zhimou
authors.initials: Z
authors.lastname: Guo
authors.forename: Chaoran
authors.initials: C
authors.lastname: Wang
authors.forename: Zhengsheng
authors.initials: Z
authors.lastname: Zhong
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Peng
authors.forename: Jun
authors.initials: J
authors.lastname: Dang
authors.forename: Yanduo
authors.initials: Y
authors.lastname: Tao
authors.forename: Xinmiao
authors.initials: X
authors.lastname: Liang
mesh_terms: D002851:Chromatography, High Pressure Liquid; D056148:Chromatography, Reverse-Phase; D031239:Lonicera / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D015394:Molecular Structure; D009930:Organic Chemicals / Q000737:chemistry / Q000302:isolation & purification*; D018547:Plant Stems / Q000737:chemistry*
chemical_list: D009930:Organic Chemicals
keywords: High-purity compound; Lonicera japonica Thunb; Traditional Chinese medicine; Two-dimensional preparation