title: Comparative study of different latent infections of herpes simplex virus type I in a murine model.
abstract: This study aims to compare the different latent infections of herpes simplex virus type I in a murine model. One hundred and twenty BALB/c mice were randomly assigned into either of three groups: intravenous inoculation group, ocular abrasion group, and intranasal inoculation group. Six weeks later, the trigeminal ganglia (TG) were removed to detect the expression of HSV-I antigen. HSV DNA in TG was also detected by polymerase chain reaction to confirm latent infection. The rate of HSV DNA in TG detected in the intravenous inoculation group was 18/22 and 22/26 in the ocular abrasion group, both of which were higher than the rate detected in the intranasal inoculation group (18/30). The expression of HSV antigen in TG in these three groups was all negative. Mortality rate in the intravenous inoculation group was 8/30, which was much higher than those of the two other groups. Intranasal virus dripping, cornea abrasion, and intravenous injection can detect latent HSV-I infection in a murine model. Compared to two other groups, the cornea abrasion group showed less severe signs, a quicker recovery rate in acute infection, and higher incidence rate of latent infection. Therefore, it is an ideal method in the presence of latent HSV-I infection.
authors.affiliation: Department of Neurology, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, People's Republic of China.
authors.forename: Wen
authors.initials: W
authors.lastname: Huang
authors.forename: Ping
authors.initials: P
authors.lastname: Zhao
authors.forename: Xiao
authors.initials: X
authors.lastname: Chen
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Gaonian
authors.initials: G
authors.lastname: Zhao
authors.forename: Mingming
authors.initials: M
authors.lastname: Xu
authors.forename: Xiuying
authors.initials: X
authors.lastname: Chen
authors.forename: Peng
authors.initials: P
authors.lastname: Xie
mesh_terms: D000818:Animals; D003315:Cornea / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D004195:Disease Models, Animal; D006561:Herpes Simplex / Q000473:pathology / Q000821:virology*; D018259:Herpesvirus 1, Human / Q000235:genetics / Q000502:physiology*; D007275:Injections, Intravenous; D051379:Mice; D008807:Mice, Inbred BALB C; D016133:Polymerase Chain Reaction; D012720:Severity of Illness Index; D012668:Trigeminal Ganglion / Q000821:virology; D017735:Virus Latency
chemical_list: D004279:DNA, Viral