title: Circulating microRNAs in the cerebrospinal fluid of patients with brain diseases.
abstract: MicroRNAs (miRNAs) are small, noncoding regulatory RNAs that regulate gene expression at the -posttranscriptional level. Although circulating miRNAs in human body fluids have recently been recognized as disease biomarkers, especially in the field of oncology, little is known about the miRNAs in cerebrospinal fluid (CSF). This chapter describes the feasibility of miRNAs in CSF as biomarkers for the diagnosis of brain diseases and the methods of miRNA isolation from CSF.
authors.affiliation: Department of Neurology and Neurological Science, Graduate School of Medicine, Tokyo Medical and Dental University, Tokyo, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Machida
authors.forename: Takuya
authors.initials: T
authors.lastname: Ohkubo
authors.forename: Takanori
authors.initials: T
authors.lastname: Yokota
mesh_terms: D015415:Biomarkers / Q000134:cerebrospinal fluid; D001707:Biopsy, Needle; D006801:Humans; D035683:MicroRNAs / Q000134:cerebrospinal fluid*; D019636:Neurodegenerative Diseases / Q000134:cerebrospinal fluid* / Q000175:diagnosis* / Q000473:pathology; D011933:Reagent Kits, Diagnostic; D060888:Real-Time Polymerase Chain Reaction; D012015:Reference Standards; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013048:Specimen Handling; D013116:Spinal Cord / Q000737:chemistry* / Q000473:pathology
chemical_list: D015415:Biomarkers; D035683:MicroRNAs; D011933:Reagent Kits, Diagnostic