title: [Adult lymphocytosis etiology by flow cytometry].
abstract: BACKGROUND
  Positive and differential diagnosis of chronic lymphocytic leukemia (CLL) is based on immunophenotyping analysis. CLL is searched whenever a persistent lymphocytosis is found.
  
  
  OBJECTIVE
  To evaluate the performance of flow cytometry in etiologic diagnosis of lymphocytosis. Could it allow us to distinguish CLL from other causes of lymphocytosis?
  
  
  METHODS
  Blood samples from 104 adult patients having a rate of lymphocytes> 5000 élé/mm3 persisting more than three months were analyzed using a large panel of monoclonal antibodies in three colors and Cell Quest software. results: Lymphoproliferative B disorder was retained in 83 cases, including 50 cases of typical CLL with Matutes score≥ 4 and 12 cases of atypical CLL with Matutes score = 3 . Diagnosis of hairy cell leukemia and follicular lymphoma were guided by the respective specific antigen expression CD103 and CD10. Large granular T lymphoma (LGL-T) was the most common etiology of lymphoid T proliferation. Unusual cases of Natural Killer (NK) and NK/T proliferations were found.
  
  
  CONCLUSIONS
  The Flow cytometry is a powerful tool to establish lymphocytosis etiological diagnosis; it avoids invasive investigations in a large number of cases.
authors.forename: Ines
authors.initials: I
authors.lastname: Safra
authors.forename: Farah
authors.initials: F
authors.lastname: Laouiti
authors.forename: Zeineb
authors.initials: Z
authors.lastname: Manai
authors.forename: Sami
authors.initials: S
authors.lastname: Zriba
authors.forename: Houda
authors.initials: H
authors.lastname: Hmida
authors.forename: Moez
authors.initials: M
authors.lastname: Mdhaffer
authors.forename: Jihene
authors.initials: J
authors.lastname: Bettaieb
authors.forename: Chaker
authors.initials: C
authors.lastname: Fouzai
authors.forename: Abdeladhim
authors.initials: A
authors.lastname: Ben Abdelahim
authors.forename: Neila
authors.initials: N
authors.lastname: Ben Romdhane
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007938:Leukemia / Q000175:diagnosis; D008218:Lymphocytosis / Q000209:etiology*; D008223:Lymphoma / Q000175:diagnosis; D008297:Male; D008875:Middle Aged