title: Construction of triglyceride biosensor based on nickel oxide-chitosan/zinc oxide/zinc hexacyanoferrate film.
abstract: A method is described for construction of an amperometric triglyceride (TG) biosensor based on co-immobilization of lipase, glycerol kinase (GK) and glycerol-3-phosphate oxidase (GPO) onto nickel oxide nanoparticles (NiONPs)-chitosan (CHIT) nanocomposite adsorbed onto zinc oxide/zinc hexacyanoferrate (ZnO-ZnHCF) hybrid film electrodeposited on the surface of an Au electrode. The NiONPs-CHIT/ZnO-ZnHCF hybrid film was characterized by cyclic voltammetry (CV), atomic force microscopy (AFM) and electrochemical impedance spectroscopy (EIS). The biosensor showed optimum response within 4 s at pH 6.0 and 35 °C, when polarized at +0.4 V against Ag/AgCl. There was a linear relationship between sensor response and triolein concentration in the range 50-700 mg/dl with sensitivity of 0.05 μA/mg/dl. The sensor was employed for determination of TG in serum. The detection limit of the biosensor was 10 mg/dl. The biosensor was evaluated with 95-96% recovery of added triolein in sera and 2% and 3% within and between batch coefficients of variation (CVs) respectively. There was a good correlation (r=0.99) between serum TG values by standard enzymic colorimetric method and the present method. The biosensor lost 50% of its initial activity after its 100 uses over a period of 180 days, when stored at 4 °C.
authors.affiliation: Department of Biochemistry, M.D. University, Rohtak 124 001, Haryana, India.
authors.forename: J
authors.initials: J
authors.lastname: Narang
authors.forename: N
authors.initials: N
authors.lastname: Chauhan
authors.forename: C S
authors.initials: CS
authors.lastname: Pundir
mesh_terms: D015374:Biosensing Techniques; D048271:Chitosan / Q000737:chemistry*; D004566:Electrodes; D009532:Nickel / Q000737:chemistry*; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D009532:Nickel; D048271:Chitosan; C028007:nickel monoxide; D015034:Zinc Oxide
keywords: Glycerol kinase; Glycerol-3-phosphate oxidase; Lipase; Nickel nanoparticles; Triglyceride