title: A conserved mechanism for centromeric nucleosome recognition by centromere protein CENP-C.
abstract: Chromosome segregation during mitosis requires assembly of the kinetochore complex at the centromere. Kinetochore assembly depends on specific recognition of the histone variant CENP-A in the centromeric nucleosome by centromere protein C (CENP-C). We have defined the determinants of this recognition mechanism and discovered that CENP-C binds a hydrophobic region in the CENP-A tail and docks onto the acidic patch of histone H2A and H2B. We further found that the more broadly conserved CENP-C motif uses the same mechanism for CENP-A nucleosome recognition. Our findings reveal a conserved mechanism for protein recruitment to centromeres and a histone recognition mode whereby a disordered peptide binds the histone tail through hydrophobic interactions facilitated by nucleosome docking.
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, Bethesda, MD 20892, USA.
authors.forename: Hidenori
authors.initials: H
authors.lastname: Kato
authors.forename: Jiansheng
authors.initials: J
authors.lastname: Jiang
authors.forename: Bing-Rui
authors.initials: BR
authors.lastname: Zhou
authors.forename: Marieke
authors.initials: M
authors.lastname: Rozendaal
authors.forename: Hanqiao
authors.initials: H
authors.lastname: Feng
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Ghirlando
authors.forename: T Sam
authors.initials: TS
authors.lastname: Xiao
authors.forename: Aaron F
authors.initials: AF
authors.lastname: Straight
authors.forename: Yawen
authors.initials: Y
authors.lastname: Bai
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001324:Autoantigens / Q000378:metabolism; D001665:Binding Sites; D002503:Centromere / Q000378:metabolism*; D000076247:Centromere Protein A; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism*; D017124:Conserved Sequence; D004330:Drosophila; D006657:Histones / Q000378:metabolism*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008969:Molecular Sequence Data; D009707:Nucleosomes / Q000378:metabolism*; D017433:Protein Structure, Secondary
chemical_list: D001324:Autoantigens; C000619700:CENPA protein, human; D000076247:Centromere Protein A; D002868:Chromosomal Proteins, Non-Histone; D006657:Histones; D009707:Nucleosomes; C060905:centromere protein C