title: Sequence-specific and visual identification of the influenza virus NS gene by azobenzene-tethered bis-peptide nucleic acid.
abstract: To rapidly and specifically identify highly virulent influenza virus strains, we prepared an azobenzene-tethered hairpin-type peptide nucleic acid, bisPNA-AZO, which has a complementary sequence against a highly conserved genomic RNA sequence within the ribonucleoprotein complex of the 2009 pandemic influenza A virus, H1N1 subtype. bisPNA-AZO recognizes the conserved virus genome sequence in a sequence-specific manner. Immobilization of bisPNA-AZO on a plate allowed capture of the target virus gene and the generation of a visual colour signal.
authors.affiliation: The Institute of Scientific and Industrial Research, Osaka University, Osaka, Japan.
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Kaihatsu
authors.forename: Shinjiro
authors.initials: S
authors.lastname: Sawada
authors.forename: Shota
authors.initials: S
authors.lastname: Nakamura
authors.forename: Takaaki
authors.initials: T
authors.lastname: Nakaya
authors.forename: Teruo
authors.initials: T
authors.lastname: Yasunaga
authors.forename: Nobuo
authors.initials: N
authors.lastname: Kato
mesh_terms: D001391:Azo Compounds / Q000737:chemistry / Q000378:metabolism*; D001483:Base Sequence; D017124:Conserved Sequence / Q000235:genetics; D005453:Fluorescence; D005814:Genes, Viral / Q000235:genetics*; D016679:Genome, Viral / Q000235:genetics; D055767:Immobilized Proteins / Q000378:metabolism; D009975:Orthomyxoviridae / Q000235:genetics*; D020135:Peptide Nucleic Acids / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D012367:RNA, Viral / Q000235:genetics; D017361:Viral Nonstructural Proteins / Q000235:genetics*
chemical_list: D001391:Azo Compounds; D055767:Immobilized Proteins; C108309:NS protein, influenza virus; D020135:Peptide Nucleic Acids; D012367:RNA, Viral; D017361:Viral Nonstructural Proteins