title: Disruption of mediator complex subunit 19 (Med19) inhibits cell growth and migration in tongue cancer.
abstract: BACKGROUND
  Mediator complex subunit 19 (Med19) is a critical subunit of the mediator complex that forms a bridge between the transcription factors and RNA polymerase II. Although it has been reported that Med19 plays an important role in stabilizing the whole mediator complex, its biological importance in tongue cancer cell proliferation and migration has not been addressed.
  
  
  METHODS
  By using MTT, BrdU incorporation, colony formation, flow cytometric, tumorigenesis and transwell assays, We tested the Med19 role on tongue cancer cell growth and migration.
  
  
  RESULTS
  We demonstrated that lentivirus-mediated Med19 knockdown could arrest tongue cancer cells at G1 phase, inhibit tongue cancer cell proliferation and migration in vitro. The tumorigenicity of Med19 short hairpin RNA (shRNA)-expressing lentivirus infected tongue cancer cells were decreased after inoculating into nude mice.
  
  
  CONCLUSIONS
  These results indicate that Med19 plays an important role in tongue cancer proliferation and migration, and suggest possible applications for tongue cancer therapy.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Guanghua College of Stomatology, Sun Yat-Sen University, 56 Lingyuanxi Road, Guangzhou 510055, China.
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Zhu
authors.forename: Wang-Xiang
authors.initials: WX
authors.lastname: Yan
authors.forename: Zhong-Wei
authors.initials: ZW
authors.lastname: Chen
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.forename: Dan
authors.initials: D
authors.lastname: Chen
authors.forename: Tong-Han
authors.initials: TH
authors.lastname: Zhang
authors.forename: Gui-Qing
authors.initials: GQ
authors.lastname: Liao
mesh_terms: D000818:Animals; D002453:Cell Cycle; D002465:Cell Movement; D049109:Cell Proliferation; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D056892:Mediator Complex / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D034741:RNA, Small Interfering / Q000235:genetics; D014062:Tongue Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: C558319:MED19 protein, human; D056892:Mediator Complex; D034741:RNA, Small Interfering