title: Measurement of hydroxyproline and nitric oxide, and comparison of sac fluid acidity in different inguinal pathologies.
abstract: OBJECTIVE
  To compare hydroxyproline and nitric oxide levels, and fluid acidity between sac tissues from inguinal hernia, hydrocele and undescended testis patients.
  
  
  METHODS
  Thirty patients were in the inguinal hernia group, 11 patients in the hydrocele group and 5 patients in the undescended testis with inguinal hernia group. Intrasaccular fluid acidity, and nitric oxide and hydroxyproline levels in sac tissues were measured and analyzed statistically between groups.
  
  
  RESULTS
  Intrasaccular fluid could be obtained from 24 inguinal hernia and 6 hydrocele patients. Acidity level (pH) was 7.5 ± 0.74 in the hernia group and 7.86 ± 0.63 in the hydrocele group (p < 0.05). All patients in the three groups were evaluated for nitric oxide and hydroxyproline. Results for hydroxyproline (mg/g dry tissue)/nitric oxide (nmol/g wet tissue) were 3.53 ± 0.54/2.02 ± 0.39 for inguinal hernia, 2.89 ± 0.9/1.92 ± 1.05 for hydrocele and 3.02 ± 1.23/2.86 ± 1.09 for undescended testis group (p > 0.05).
  
  
  CONCLUSIONS
  Hydroxyproline and nitric oxide pathways do not seem to be responsible for the different characteristics of inguinal diseases. However, differences in the acidity of sac fluid may be evidence of the different mechanisms of hernia and hydrocele formation.
authors.affiliation: Pediatric Surgery Department, TCSB Diskapi Children's Education and Research Hospital, Yenimahalle, Ankara, Turkey. Electronic address: ysenayli@gmail.com.
authors.forename: Atilla
authors.initials: A
authors.lastname: Şenaylı
authors.forename: Fatih
authors.initials: F
authors.lastname: Ekici
authors.forename: Resul
authors.initials: R
authors.lastname: Yılmaz
authors.forename: Hasan
authors.initials: H
authors.lastname: Erdoğan
mesh_terms: D000143:Acids / Q000378:metabolism*; D001202:Ascitic Fluid / Q000378:metabolism; D002675:Child, Preschool; D003456:Cryptorchidism / Q000378:metabolism*; D005260:Female; D006552:Hernia, Inguinal / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D006909:Hydroxyproline / Q000378:metabolism*; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism*; D006848:Testicular Hydrocele / Q000378:metabolism*
chemical_list: D000143:Acids; D009569:Nitric Oxide; D006909:Hydroxyproline
keywords: Hernia; Hydrocele; Hydroxyproline; Nitric oxide; Peritoneal fluid; Undescended testis