title: Toxic effect of cooking oil fumes in primary fetal pulmonary type II-like epithelial cells.
abstract: Epidemiological studies indicated that there is an increased risk of respiratory tract cancer among cooks and bakers. The cooking oil fumes are believed to conduct this risk, and many studies have focused on evaluating the mutagenicity and finding the mutagenic components in oil fumes. COFs contains two major classes of compounds. One class consists of polycyclic aromatic hydrocarbons (PAHs), such as benzo[a]pyrene, benzo[b]fluoranthene, fluoranthene, and benzo[g,h,i]perylene. BaP is a known immunosuppressant. It can also alter cell cycle progression, induce inflammation, and impair DNA repair and apoptotic processes leading to aberrant cellular functioning. This study investigates the effect of toxicity of cooking oil fumes (COFs) in primary ICR mice' fetal lung type II-like epithelium cells (AEC II). The cells were cultured in different concentrations (0, 12.5, 25, 50, 100, and 200μg/ml) of COFs for different time periods. The results showed that cell viability decreased in a dose- and time- dependent manner, which is accompanied by increased malondialdehyde (MDA) level and decreased superoxide dismutase (SOD) and glutathione (GSH) activities. Moreover, comet assay suggested DNA damage, as well as increased production of DNA adducts induced by PAHs. The present study also shows that COFs may disturb cell cycles even at a very low dose. In summary, the present study indicates that COFs may lead to toxicity in AEC II cells.
authors.affiliation: School of Public Health, Anhui Medical University, Hefei, China. Electronic address: qshq@163.com.
authors.forename: Jiyu
authors.initials: J
authors.lastname: Cao
authors.affiliation: School of Public Health, Anhui Medical University, Hefei, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Ding
authors.affiliation: School of Public Health, Anhui Medical University, Hefei, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Public Health, Anhui Medical University, Hefei, China.
authors.forename: Daojun
authors.initials: D
authors.lastname: Chen
authors.affiliation: School of Public Health, Anhui Medical University, Hefei, China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Guo
authors.affiliation: School of Public Health, Anhui Medical University, Hefei, China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Liang
authors.affiliation: School of Public Health, Anhui Medical University, Hefei, China.
authors.forename: Zhewei
authors.initials: Z
authors.lastname: Feng
authors.affiliation: School of Public Health, Anhui Medical University, Hefei, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Che
mesh_terms: D056809:Alveolar Epithelial Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D000818:Animals; D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003296:Cooking; D018736:DNA Adducts / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000378:metabolism; D008168:Lung / Q000187:drug effects* / Q000196:embryology / Q000378:metabolism / Q000473:pathology; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D018384:Oxidative Stress / Q000187:drug effects; D000074241:Peanut Oil; D010938:Plant Oils / Q000633:toxicity*; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors; D014835:Volatilization
chemical_list: D018736:DNA Adducts; D000074241:Peanut Oil; D010938:Plant Oils; D011084:Polycyclic Aromatic Hydrocarbons; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: AEC II, MDA; COFs; Comet assay; GSH; SOD