title: Acetone-Butanol-Ethanol (ABE) production in fermentation of enzymatically hydrolyzed cassava flour by Clostridium beijerinckii BA101 and solvent separation.
abstract: Cassava constitutes an abundant substrate in tropical regions. The production of butanol in ABE fermentation by Clostridium beijerinckii BA101 using cassava flour (CF) was scaled-up to bioreactor level (5 L). Optimized fermentation conditions were applied; that is, 40℃, 60 g/l CF, and enzymatic pretreatment of the substrate. The batch fermentation profile presented an acidogenic phase for the first 24 h and a solventogenic phase afterwards. An average of 37.01 g/l ABE was produced after 83 h, with a productivity of 0.446 g/l/h. Butanol production was 25.71 g/l with a productivity of 0.310 g/l/h, high or similar to analogous batch processes described for other substrates. Solvent separation by different combinations of fractioned and azeotropic distillation and liquid-liquid separation were assessed to evaluate energetic and economic costs in downstream processing. Results suggest that the use of cassava as a substrate in ABE fermentation could be a cost-effective way of producing butanol in tropical regions.
authors.affiliation: Food and Water Microbiology Laboratory, Faculty of Microbiology, UCR, 2060 San José, Costa Rica.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Lépiz-Aguilar
authors.forename: Carlos E
authors.initials: CE
authors.lastname: Rodríguez-Rodríguez
authors.forename: María Laura
authors.initials: ML
authors.lastname: Arias
authors.forename: Giselle
authors.initials: G
authors.lastname: Lutz
mesh_terms: D000096:Acetone / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods; D000440:Butanols / Q000378:metabolism*; D005591:Chemical Fractionation; D046970:Clostridium beijerinckii / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005433:Flour; D002365:Manihot; D012997:Solvents
chemical_list: D000440:Butanols; D012997:Solvents; D000096:Acetone; D000431:Ethanol