title: Sulfated polysaccharide of Caesalpinia ferrea inhibits herpes simplex virus and poliovirus.
abstract: Herpes simplex virus (HSV) is one of the most regular human pathogens, being a public health problem, and causal agent of several diseases. Poliovirus (PV) is an enteric virus and about 1% of infected individuals develop paralytic poliomyelitis due to viral invasion of the central nervous system and destruction of motor neurons. This work evaluated the activity of a sulfated polysaccharide of Caesalpinia ferrea (SPLCf) in HSV and PV replication. The antiviral effect of SPLCf at varying concentrations was tested by plaque assay under several protocols, such as time-of-addition, adsorption and penetration inhibition and virucidal. Syntheses of viral protein and nucleic acid were also monitored by the immunofluorescence assay and PCR. The SPLCf inhibited virus adsorption and steps after penetration, and inhibited the synthesis of viral protein. Virucidal effect was also shown and nucleic acid synthesis was concurrent with positive results. Our findings suggested that the substance with low toxicity represent a potential viral inhibitor.
authors.affiliation: Departamento de Microbiologia, CCB, Universidade Estadual de Londrina, CEP 86051-990, Londrina, Paraná, Brazil.
authors.forename: Nayara
authors.initials: N
authors.lastname: Lopes
authors.forename: Lígia Carla
authors.initials: LC
authors.lastname: Faccin-Galhardi
authors.forename: Samantha Fernandes
authors.initials: SF
authors.lastname: Espada
authors.forename: Arcelina Cunha
authors.initials: AC
authors.lastname: Pacheco
authors.forename: Nágila Maria Pontes Silva
authors.initials: NM
authors.lastname: Ricardo
authors.forename: Rosa Elisa Carvalho
authors.initials: RE
authors.lastname: Linhares
authors.forename: Carlos
authors.initials: C
authors.lastname: Nozawa
mesh_terms: D000998:Antiviral Agents / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D029911:Caesalpinia / Q000737:chemistry*; D002460:Cell Line; D006801:Humans; D019906:Nuclear Magnetic Resonance, Biomolecular; D010936:Plant Extracts / Q000737:chemistry*; D017955:Poliovirus / Q000187:drug effects*; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D018139:Simplexvirus / Q000187:drug effects*; D017550:Spectroscopy, Fourier Transform Infrared; D010948:Viral Plaque Assay; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D010936:Plant Extracts; D011134:Polysaccharides
keywords: Antiviral; Caesalpinia ferrea; Caesalpinia ferrea sulfated polysaccharide; HSV; Herpesvirus; PV; Poliovirus; SPLCf; Sulfated polysaccharide; herpes simplex virus; poliovirus