title: Aptamer functionalized gold nanoparticles based fluorescent probe for the detection of mercury (II) ion in aqueous solution.
abstract: A method is proposed for the detection of Hg(2+) using Hg(2+) specific DNA (MSD) functionalized gold nanoparticles (AuNPs) based on the formation of T-Hg(2+)-T complex and the excellent quenching fluorescence property of AuNPs. The MSD is rich in thymine (T) and readily forms T-Hg(2+)-T configuration in the presence of Hg(2+). The MSD which is labeled with a fluorescein (FAM) at the 3'-end and a thiol at the 5'-end is bounded to the AuNPs through Au-S covalent bonds to form the probes (AuNPs-MSD). Hg(2+) detection can be easily realized by monitoring the change of fluorescence signal of AuNPs-MSD probes. Hg(2+) can be detected in a range of 0.02-1.0 µM with a detection limit of 16 nM. Besides, the assay shows excellent selectivity for Hg(2+) over other metal cations such as Fe(3+), Ca(2+), Mg(2+), Mn(2+), Cr(3+), Ni(2+), Cu(2+), Co(2+) and Pb(2+). The major advantages of this Hg(2+) assay are its water-solubility, simplicity, low cost and high sensitivity. Moreover, this method provides a potentially useful method for the Hg(2+) detection in aqueous solution.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine, College of Chemistry and Molecular Sciences, Wuhan Institute of Biotechnology, Wuhan University, Wuhan 430072, China.
authors.forename: Daidi
authors.initials: D
authors.lastname: Tan
authors.forename: Yue
authors.initials: Y
authors.lastname: He
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Xing
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhao
authors.forename: Hongwu
authors.initials: H
authors.lastname: Tang
authors.forename: Daiwen
authors.initials: D
authors.lastname: Pang
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D004785:Environmental Pollutants / Q000032:analysis* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D008628:Mercury / Q000032:analysis* / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D012996:Solutions; D013941:Thymine / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D004785:Environmental Pollutants; D005456:Fluorescent Dyes; D012996:Solutions; D006046:Gold; D008628:Mercury; D013941:Thymine