title: A dual role for the βPS integrin myospheroid in mediating Drosophila embryonic macrophage migration.
abstract: Throughout embryonic development, macrophages not only act as the first line of defence against infection but also help to sculpt organs and tissues of the embryo by removing dead cells and secreting extracellular matrix components. Key to their function is the ability of embryonic macrophages to migrate and disperse throughout the embryo. Despite these important developmental functions, little is known about the molecular mechanisms underlying embryonic macrophage migration in vivo. Integrins are key regulators of many of the adult macrophage responses, but their role in embryonic macrophages remains poorly characterized. Here, we have used Drosophila macrophages (haemocytes) as a model system to address the role of integrins during embryonic macrophage dispersal in vivo. We show that the main βPS integrin, myospheroid, affects haemocyte migration in two ways; by shaping the three-dimensional environment in which haemocytes migrate and by regulating the migration of haemocytes themselves. Live imaging revealed a requirement for myospheroid within haemocytes to coordinate the microtubule and actin dynamics, and to enable haemocyte developmental dispersal, contact repulsion and inflammatory migration towards wounds.
authors.affiliation: Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK.
authors.forename: Kate
authors.initials: K
authors.lastname: Comber
authors.forename: Sven
authors.initials: S
authors.lastname: Huelsmann
authors.forename: Iwan
authors.initials: I
authors.lastname: Evans
authors.forename: Besaid J
authors.initials: BJ
authors.lastname: Sánchez-Sánchez
authors.forename: Andrew
authors.initials: A
authors.lastname: Chalmers
authors.forename: Rolf
authors.initials: R
authors.lastname: Reuter
authors.forename: Will
authors.initials: W
authors.lastname: Wood
authors.forename: Maria D
authors.initials: MD
authors.lastname: Martín-Bermudo
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology; D004330:Drosophila / Q000166:cytology* / Q000196:embryology; D029721:Drosophila Proteins / Q000502:physiology*; D039641:Integrin beta Chains / Q000378:metabolism*; D008264:Macrophages / Q000166:cytology
chemical_list: D029721:Drosophila Proteins; D039641:Integrin beta Chains
keywords: Integrins; Macrophage; Migration