title: Expression of p16 protein in lesional and perilesional condyloma acuminata and bowenoid papulosis: clinical significance and diagnostic implications.
abstract: BACKGROUND
  The histopathological and clinical overlapping features between condyloma acuminata and bowenoid papulosis can present a diagnostic challenge and we sought to determine if immunochemistry can be helpful in this setting.
  
  
  OBJECTIVE
  In this study, we evaluate the specificity and sensitivity of p16 immunohistochemistry in condyloma and bowenoid papulosis lesions compared with uninvolved perilesional skin and discuss the possible clinical implications.
  
  
  METHODS
  A total of 36 skin biopsy specimens (24 samples of condyloma and 12 samples of bowenoid papulosis with adjacent uninvolved perilesional skin) were stained with an antibody to p16 protein.
  
  
  RESULTS
  In all, 75% of condyloma lesions showed sporadic and focal positive staining for p16 protein. All cases of bowenoid papulosis showed diffuse positive staining with p16 protein. Normal-appearing adjacent skin was negative in all cases.
  
  
  CONCLUSIONS
  Studies with a larger number of cases are needed to confirm our data.
  
  
  CONCLUSIONS
  This immunostain has high sensitivity and specificity for the detection of bowenoid papulosis. Although p16 is expressed in both conditions, the staining pattern in bowenoid papulosis is strongly and diffusely positive involving the full thickness of the epidermis compared with the mostly focal or sporadic pattern observed in condyloma.
authors.affiliation: Ackerman Academy of Dermatopathology, New York, New York 10016, USA.
authors.forename: Viktoryia
authors.initials: V
authors.lastname: Kazlouskaya
authors.forename: Elina
authors.initials: E
authors.lastname: Shustef
authors.forename: Sherihan H
authors.initials: SH
authors.lastname: Allam
authors.forename: Karan
authors.initials: K
authors.lastname: Lal
authors.forename: Dirk
authors.initials: D
authors.lastname: Elston
mesh_terms: D000328:Adult; D001913:Bowen's Disease / Q000737:chemistry* / Q000175:diagnosis; D003218:Condylomata Acuminata / Q000175:diagnosis*; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000032:analysis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity; D012867:Skin / Q000737:chemistry*; D012878:Skin Neoplasms / Q000737:chemistry* / Q000175:diagnosis
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16
keywords: CDK; HPV; SCC; atypical features; bowenoid papulosis; condyloma acuminata; cyclin-dependent kinase; human papillomavirus; neoplasia; p16; squamous cell carcinoma