title: Oral cancer secretome: identification of cancer-associated proteins.
abstract: This study aims to identify cancer-associated proteins in the secretome of oral cancer cell lines. We have successfully established four primary cell cultures of normal cells with a limited lifespan without human telomerase reverse transcriptase (hTERT) immortalization. The secretome of these primary cell cultures were compared with that of oral cancer cell lines using 2DE. Thirty five protein spots were found to have changed in abundance. Unambiguous identification of these proteins was achieved by MALDI TOF/TOF. In silico analysis predicted that 24 of these proteins were secreted via classical or nonclassical mechanisms. The mRNA expression of six genes was found to correlate with the corresponding protein abundance. Ingenuity Pathway Analysis (IPA) core analysis revealed that the identified proteins were relevant in, and related to, cancer development with likely involvements in tumor growth, metastasis, hyperproliferation, tumorigenesis, neoplasia, hyperplasia, and cell transformation. In conclusion, we have demonstrated that a comparative study of the secretome of cancer versus normal cell lines can be used to identify cancer-associated proteins.
authors.affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Hong-Yun
authors.initials: HY
authors.lastname: Chang
authors.forename: Seen-Yii
authors.initials: SY
authors.lastname: Hor
authors.forename: Kue-Peng
authors.initials: KP
authors.lastname: Lim
authors.forename: Rosnah Binti
authors.initials: RB
authors.lastname: Zain
authors.forename: Sok-Ching
authors.initials: SC
authors.lastname: Cheong
authors.forename: Mariati Abdul
authors.initials: MA
authors.lastname: Rahman
authors.forename: Saiful Anuar
authors.initials: SA
authors.lastname: Karsani
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D016923:Cell Death / Q000502:physiology; D048708:Cell Growth Processes / Q000502:physiology; D002461:Cell Line, Transformed; D045744:Cell Line, Tumor; D003198:Computer Simulation; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D010446:Peptide Fragments / Q000032:analysis; D060066:Protein Interaction Maps; D020543:Proteome / Q000032:analysis* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D053719:Tandem Mass Spectrometry
chemical_list: D014408:Biomarkers, Tumor; D010446:Peptide Fragments; D020543:Proteome; D012333:RNA, Messenger
keywords: Oral cancer; Proteomics; Secretome