title: Study of trans-trans farnesol effect on hyphae formation by Yarrowia lipolytica.
abstract: Dimorphism is an ability of certain fungi related to its adaptation to the environment and provides a selective advantage under stress conditions and is associated to the development of human diseases. Hyphae inducing- and inhibitory-effect of farnesol on hyphae formation by the dimorphic yeast Yarrowia lipolytica was evaluated through digital image analysis. The agitation speed of the culture was the most effective hyphae inducer in comparison to bovine calf serum and N-acetylglucosamine. In low agitation system, bovine calf serum was more effective for hyphae formation inducing 57 % of hyphae transition. Farnesol inhibited hyphae formation even in low concentration (300 μM) and this effect increased with increasing concentrations. In the presence of N-acetylglucosamine, this effect was more evident in comparison to the presence of bovine calf serum, which might have protected the cells from farnesol. Digital image analysis was an important tool to evaluate this phenomenon.
authors.affiliation: EQ, Departamento de Engenharia Bioquímica, Centro de Tecnologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-909, Brazil.
authors.forename: Patrícia Martins Botelho
authors.initials: PM
authors.lastname: Nunes
authors.forename: Silvia Maria
authors.initials: SM
authors.lastname: da Rocha
authors.forename: Priscilla Filomena Fonseca
authors.initials: PF
authors.lastname: Amaral
authors.forename: Maria Helena Miguez
authors.initials: MH
authors.lastname: da Rocha-Leão
mesh_terms: D003470:Culture Media; D005204:Farnesol / Q000494:pharmacology*; D025301:Hyphae / Q000254:growth & development*; D025062:Yarrowia / Q000187:drug effects* / Q000254:growth & development
chemical_list: D003470:Culture Media; D005204:Farnesol