title: [A new flavanol glycoside from Phymatopteris hastata with effect on glucose metabolism].
abstract: By repeated column chromatography, including silica gel, macroporous resin, and preparative HPLC, a new compound (1) was isolated and purified. On the basis of spectroscopic methods, the structure of 1 was elucidated as ( - ) -epiafzelechin-3, 5-di-O-beta-D-apiofuranoside (1). In the bioassay screening experiments, glucose consumption assays in IR HepG2 cells and colorimetric assay of surface GLUT4myc translocation were used to assess the effects on glucose metabolism of compound 1. Both compound 1 and its derivatives--naringin could improve glucose consumption in IR HepG2 cells and enhance GLUT4 translocation in skeletal muscle cell L6myc in a dose-dependent manner, indicating that these two compouds showed potential anti-diabetic activities in vitro.
authors.affiliation: School of Pharmacy, Research Center of Basic Medical Sciences, Tianjin Key Laboratory on Technologies Enabling Development Clinical Therapeutics and Diagnostics, Tianjin Medical University, Tianjin 300070, China. jane6386812@126.com
authors.forename: Sheng-Nan
authors.initials: SN
authors.lastname: Ma
authors.forename: Shi-Lian
authors.initials: SL
authors.lastname: Duan
authors.forename: Mei-Na
authors.initials: MN
authors.lastname: Jin
authors.forename: Hong-Quan
authors.initials: HQ
authors.lastname: Duan
mesh_terms: D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D005947:Glucose / Q000378:metabolism*; D051275:Glucose Transporter Type 4 / Q000378:metabolism; D006027:Glycosides / Q000494:pharmacology*; D056945:Hep G2 Cells; D006801:Humans; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D029621:Polypodiaceae / Q000737:chemistry*; D021381:Protein Transport / Q000187:drug effects
chemical_list: D051275:Glucose Transporter Type 4; D006027:Glycosides; D007004:Hypoglycemic Agents; C120647:epiafzelechin; C581792:epiafzelechin-3,5-di-O-beta-D-apiofuranoside; D002392:Catechin; D005947:Glucose