title: SecA2 is not required for secretion of the surface autolysin IspC in Listeria monocytogenes serotype 4b.
abstract: Listeria monocytogenes is one of several Gram-positive bacteria known to contain an auxiliary ATPase (SecA2) involved in the Sec secretion of a subset of proteins important to bacterial pathogenesis, including autolysins. It is not known if IspC, a novel surface-associated autolysin essential for full virulence of L. monocytogenes serotype 4b, is SecA2-dependent for secretion. By creating a secA2 gene deletion (ΔsecA2) mutant from the wild type (WT) L. monocytogenes serotype 4b strain, in combination with the proteomic analysis of surface proteins and those secreted into the medium from both the mutant and the WT, we confirmed previous findings that two autolysins (p60 and NamA) are SecA2-dependent for secretion. However, this approach did not identify IspC as one of the surface proteins affected by the SecA2 deletion. Further experiments with immunofluorescence microscopy and Western blotting indicated that IspC was well displayed on the surface of both the ΔsecA2 mutant and WT cells, while p60 was not, clearly indicating that the secretion of IspC is not attributed to the SecA2 pathway. This finding sets IspC apart from other autolysins involved in virulence, such as p60 and NamA, in that SecA2 is not required for IspC secretion.
authors.affiliation: Canadian Food Inspection Agency, Ottawa Laboratory Fallowfield, Ottawa, Ontario, Canada; Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Ontario, Canada.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Ronholm
authors.forename: Cathy X Y
authors.initials: CX
authors.lastname: Zhang
authors.forename: Xudong
authors.initials: X
authors.lastname: Cao
authors.forename: Min
authors.initials: M
authors.lastname: Lin
mesh_terms: D000251:Adenosine Triphosphatases / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D017353:Gene Deletion; D008089:Listeria monocytogenes / Q000235:genetics / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D009238:N-Acetylmuramoyl-L-alanine Amidase / Q000378:metabolism*; D000069816:SEC Translocation Channels; D000081416:SecA Proteins; D037521:Virulence Factors / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; D000069816:SEC Translocation Channels; D037521:Virulence Factors; D009238:N-Acetylmuramoyl-L-alanine Amidase; D000251:Adenosine Triphosphatases; D000081416:SecA Proteins
keywords: IspC; Listeria monocytogenes; SecA2; Secretion