title: Antifungal activity, biofilm-controlling effect, and biocompatibility of poly(N-vinyl-2-pyrrolidinone)-grafted denture materials.
abstract: Colonization and biofilm-formation of Candida species on denture surfaces cause Candida-associated denture stomatitis (CADS), a common, recurring disease affecting up to 67% of denture wearers. We developed poly(N-vinyl-2-pyrrolidinone)-grafted denture materials that can be repeatedly recharged with various antifungal drugs to achieve long-term antifungal and biofilm-controlling effects. The monomer, N-vinyl-2-pyrrolidinone (NVP), was grafted onto poly(methyl methacrylate) denture resins through plasma-initiated grafting polymerization. The physical properties and biocompatibility of the resulting resins were not negatively affected by the presence of up to 7.92% of grafted poly (N-vinyl-2-pyrrolidinone) (PNVP). Miconazole and chlorhexidine digluconate (CD) were used as model antifungal drugs. PNVP grafting significantly increased the drug absorption capability of the resulting denture materials. Further, the new materials showed sustained drug release and provided antifungal effects for weeks (in the case of CD) to months (in the case of miconazole). The drug-depleted resins could be recharged with the same or a different class of antifungal drug to further extend antifungal duration. If needed, drugs on the PNVP-grafted denture materials could be "washed out" (quenched) by treating with PNVP aqueous solutions to stop drug release. These results point to great potentials of the new materials in controlling biofilm-formation in a wide range of device-related applications.
authors.affiliation: Medetech Development Corporation, 12527 Mukilteo Speedway STE 103, Lynnwood, WA, 98087, United States.
authors.forename: Xinbo
authors.initials: X
authors.lastname: Sun
authors.forename: Zhengbing
authors.initials: Z
authors.lastname: Cao
authors.forename: Chih-Ko
authors.initials: CK
authors.lastname: Yeh
authors.forename: Yuyu
authors.initials: Y
authors.lastname: Sun
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000737:chemistry / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology*; D018441:Biofilms; D002176:Candida albicans / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D003764:Dental Materials / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D008826:Microbial Sensitivity Tests; D041681:NIH 3T3 Cells; D011108:Polymers / Q000737:chemistry*; D011760:Pyrrolidinones / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship
chemical_list: D000935:Antifungal Agents; D001672:Biocompatible Materials; D003764:Dental Materials; D011108:Polymers; D011760:Pyrrolidinones; C042670:N-vinyl-2-pyrrolidinone