title: Common evolutionary origin for the rotor domain of rotary ATPases and flagellar protein export apparatus.
abstract: The V1- and F1- rotary ATPases contain a rotor that rotates against a catalytic A3B3 or α3β3 stator. The rotor F(1-γ) or V1-DF is composed of both anti-parallel coiled coil and globular-loop parts. The bacterial flagellar type III export apparatus contains a V1/F1-like ATPase ring structure composed of FliI6 homo-hexamer and FliJ which adopts an anti-parallel coiled coil structure without the globular-loop part. Here we report that FliJ of Salmonella enterica serovar Typhimurium shows a rotor like function in Thermus thermophilus A3B3 based on both biochemical and structural analysis. Single molecular analysis indicates that an anti-parallel coiled-coil structure protein (FliJ structure protein) functions as a rotor in A3B3. A rotary ATPase possessing an F1-γ-like protein generated by fusion of the D and F subunits of V1 rotates, suggesting F(1-γ) could be the result of a fusion of the genes encoding two separate rotor subunits. Together with sequence comparison among the globular part proteins, the data strongly suggest that the rotor domains of the rotary ATPases and the flagellar export apparatus share a common evolutionary origin.
authors.affiliation: Department of Molecular Biosciences, Kyoto Sangyo University, Motoyama Kamigamo, Kita-ku, Kyoto, Japan.
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Kishikawa
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Ibuki
authors.forename: Shuichi
authors.initials: S
authors.lastname: Nakamura
authors.forename: Astuko
authors.initials: A
authors.lastname: Nakanishi
authors.forename: Tohru
authors.initials: T
authors.lastname: Minamino
authors.forename: Tomoko
authors.initials: T
authors.lastname: Miyata
authors.forename: Keiichi
authors.initials: K
authors.lastname: Namba
authors.forename: Hiroki
authors.initials: H
authors.lastname: Konno
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ueno
authors.forename: Katsumi
authors.initials: K
authors.lastname: Imada
authors.forename: Ken
authors.initials: K
authors.lastname: Yokoyama
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D001426:Bacterial Proteins / Q000378:metabolism*; D020285:Cryoelectron Microscopy; D019143:Evolution, Molecular; D005407:Flagella / Q000378:metabolism*; D008958:Models, Molecular; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D012486:Salmonella typhimurium / Q000201:enzymology*; D016963:Thermus thermophilus
chemical_list: D001426:Bacterial Proteins; C069253:fliJ protein, Bacteria; D000251:Adenosine Triphosphatases