title: Radioimmunoassay for the quantification of cGMP levels in cells and tissues.
abstract: Radioimmunoassay is an established method to determine the amount of a specific substance in a given cell or tissue sample. Commercially available RIA or Elisa are very cost intensive. Here, we describe the generation of radioactive cGMP tracer and the quantification of cGMP. Although working with radioactive material requires experience and care, this method is very sensitive and rather cheap, once it is established.
authors.affiliation: Physiologisches Institut I, Universität Würzburg, Würzburg, Germany.
authors.forename: Ronald
authors.initials: R
authors.lastname: Jäger
authors.forename: Dieter
authors.initials: D
authors.lastname: Groneberg
authors.forename: Barbara
authors.initials: B
authors.lastname: Lies
authors.forename: Noomen
authors.initials: N
authors.lastname: Bettaga
authors.forename: Michaela
authors.initials: M
authors.lastname: Kümmel
authors.forename: Andreas
authors.initials: A
authors.lastname: Friebe
mesh_terms: D002606:Charcoal / Q000032:analysis / Q000737:chemistry; D006152:Cyclic GMP / Q000032:analysis / Q000737:chemistry*; D054879:Halogenation; D006801:Humans; D011863:Radioimmunoassay / Q000379:methods*
chemical_list: D002606:Charcoal; D006152:Cyclic GMP