title: Effect of antibiotic treatment on intestinal microbial and enzymatic development in postnatally overfed obese rats.
abstract: OBJECTIVE
  To investigate the effect of the microbiota-induced changes and early overfeeding after amoxicillin administration (a) in suckling pups via their dams up to 15 days of lactation and (b) in weaned pups on intestinal microbial/functional adaptability and obesity development in male Sprague-Dawley rats.
  
  
  METHODS
  Postnatal nutrition was elicited by adjusting the number of pups in the nest to 4 (small litters [SLs]) and 10 (normal litters [NLs]), while from days 21 to 40, both groups were fed with a standard diet. The numbers of Bacteroides/Prevotella (BAC) and Lactobacillus/Enterococcus (LAB) in the jejunum and colon were determined by fluorescence in situ hybridization technique, and jejunal alkaline phosphatase (AP), α-glucosidase and aminopeptidase activity was assayed histochemically.
  
  
  RESULTS
  On day 40, the SL in comparison with NL animals displayed excess weight/fat gain accompanied by higher LAB and lower numbers of BAC, and with permanently higher AP activity. Moreover, these acquired changes continued in SL vs. NL rats and were not influenced by antibiotic treatment, which induced significant decrease in the quantity of LAB and BAC.
  
  
  CONCLUSIONS
  These findings highlight the role of early life overfeeding upon the gut microbial/functional ontogeny and allow to distinguish their potential involvement in later risk of obesity.
authors.affiliation: Department of Developmental Physiology, Institute of Animal Physiology, Slovak Academy of Sciences, Šoltésovej 4-6, 040 01 Košice, Slovak Republic. mozes@saske.sk
authors.forename: Štefan
authors.initials: Š
authors.lastname: Mozeš
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Šefcíková
authors.forename: Dobroslava
authors.initials: D
authors.lastname: Bujnáková
authors.forename: Lubomír
authors.initials: L
authors.lastname: Racek
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000626:Aminopeptidases / Q000378:metabolism; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000009:adverse effects*; D001439:Bacteroides / Q000187:drug effects / Q000254:growth & development; D004032:Diet; D016983:Enterococcus / Q000187:drug effects / Q000254:growth & development; D005260:Female; D017404:In Situ Hybridization, Fluorescence; D007422:Intestines / Q000187:drug effects / Q000201:enzymology* / Q000382:microbiology*; D007778:Lactobacillus / Q000187:drug effects / Q000254:growth & development; D008297:Male; D064307:Microbiota; D009765:Obesity / Q000382:microbiology* / Q000473:pathology; D044343:Overnutrition / Q000473:pathology; D018720:Prevotella / Q000187:drug effects / Q000254:growth & development; D051381:Rats; D017207:Rats, Sprague-Dawley; D014886:Weaning; D015430:Weight Gain / Q000187:drug effects; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D000469:Alkaline Phosphatase; D000520:alpha-Glucosidases; D000626:Aminopeptidases