title: Non-sterilized fermentative co-production of poly(γ-glutamic acid) and fibrinolytic enzyme by a thermophilic Bacillus subtilis GXA-28.
abstract: Poly(γ-glutamic acid), as a naturally occurring homopolymer, is widely used in industry, agriculture, food and medicine. Fibrinolytic enzyme has a great potential for the prevention and/or treatment of vascular diseases caused by fibrin clots. Co-production of γ-PGA and fibrinolytic enzyme by Bacillus subtilis GXA-28 (CCTCC M 2012347) from soybean residue using cane molasses and monosodium glutamate waste liquor under sterilized and non-sterilized condition were investigated. It was observed that total sugar from cane molasses of 3% (w/w) and glutamate from monosodium glutamate waste liquor of 2% (w/w) were favorable for γ-PGA and fibrinolytic enzyme co-production at pH 7.0 and 45°C. Based on the optimal medium, the γ-PGA and fibrinolytic activity reached 103.5 g/kg-substrates at 22 h and 986 U/g-substrates at 24h under non-sterilized condition, respectively. To our knowledge, the yield of γ-PGA was highest in all reported literatures.
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning 530004, Guangxi, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zeng
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Lin
authors.initials: L
authors.lastname: Shu
authors.forename: Juyang
authors.initials: J
authors.lastname: Yi
authors.forename: Guiguang
authors.initials: G
authors.lastname: Chen
authors.forename: Zhiqun
authors.initials: Z
authors.lastname: Liang
mesh_terms: D001412:Bacillus subtilis / Q000378:metabolism*; D004798:Enzymes / Q000096:biosynthesis*; D005285:Fermentation; D005342:Fibrinolysis; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000096:biosynthesis
chemical_list: D004798:Enzymes; C511775:poly(gamma-glutamic acid); D011099:Polyglutamic Acid
keywords: Bacillus subtilis; Fibrinolytic enzyme; Non-sterilized; Poly(γ-glutamic acid); Solid-state fermentation