title: Colonization of Madagascar periwinkle (Catharanthus roseus), by endophytes encoding gfp marker.
abstract: This study reports the introduction of gfp marker in two endophytic bacterial strains (Pantoea agglomerans C33.1, isolated from cocoa, and Enterobacter cloacae PR2/7, isolated from citrus) to monitor the colonization in Madagascar perinwinkle (Catharanthus roseus). Stability of the plasmid encoding gfp was confirmed in vitro for at least 72 h of bacterial growth and after the colonization of tissues, under non-selective conditions. The colonization was observed using fluorescence microscopy and enumeration of culturable endophytes in inoculated perinwinkle plants that grew for 10 and 20 days. Gfp-expressing strains were re-isolated from the inner tissues of surface-sterilized roots and stems of inoculated plants, and the survival of the P. agglomerans C33:1gfp in plants 20 days after inoculation, even in the absence of selective pressure, suggests that is good colonizer. These results indicated that both gfp-tagged strains, especially P. agglomerans C33.1, may be useful tools to deliver enzymes or other proteins in plant.
authors.affiliation: Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, PO Box 83, Piracicaba, São Paulo 13400-970, Brazil. adalgisa@cnpso.embrapa.br
authors.forename: Adalgisa Ribeiro
authors.initials: AR
authors.lastname: Torres
authors.forename: Welington Luiz
authors.initials: WL
authors.lastname: Araújo
authors.forename: Luciana
authors.initials: L
authors.lastname: Cursino
authors.forename: Priscilla
authors.initials: P
authors.lastname: de Barros Rossetto
authors.forename: Mateus
authors.initials: M
authors.lastname: Mondin
authors.forename: Mariangela
authors.initials: M
authors.lastname: Hungria
authors.forename: João Lúcio
authors.initials: JL
authors.lastname: Azevedo
mesh_terms: D030008:Catharanthus / Q000382:microbiology*; D060026:Endophytes / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D016972:Enterobacter cloacae / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D049452:Green Fluorescent Proteins / Q000235:genetics* / Q000378:metabolism; D008856:Microscopy, Fluorescence; D020636:Pantoea / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D018517:Plant Roots / Q000382:microbiology; D018547:Plant Stems / Q000382:microbiology; D010957:Plasmids; D014169:Transformation, Bacterial
chemical_list: D049452:Green Fluorescent Proteins