title: [Content comparison of buddleoside and pectolinarin in Cirsium japonicum, C. leo and C. leducei].
abstract: OBJECTIVE
  To compare Cirsium japonicum characteristics with C. leo and C. leducei, along with the content of buddleoside and pectolinarin, and lay the foundation for the quality control of C. japonicum.
  
  
  METHODS
  Samples were collected and the relevant drugs were bought. The samples were divided into root, stem, leaf and flower, and the content of buddleoside and pectolinarin was determine by the HPLC. Chromatographic column: Waters XBridge C18 (4.6 mm x 250 mm), mobile phase: methanol-water (45: 55), measurement wavelength: 326 nm, flow rate: 0.8 mL x min(-1), column temperature: 30 degrees C. RESULT AND CONDUSION: Standard curve equation of buddleoside: Y = 74 064X-47 748, R2 = 0.991. Standard curve equation of pectolinarin: Y = 1 711 64X - 180 707, R2 = 0.999. The content of buddleoside: C. japonicum leaf was 1.987 3%, C. leo leaf 1.412 2%, C. leducei leaf 0.149 2%. The content of buddleoside was lower in root and stem. Pectolinarin was not detected in the C. japonicum and C. leo. The pectolinarin content was 0.069 0% in C. leducei leaf.
authors.affiliation: Life Science and Engineering College of Southwest Jiaotong University, Chengdu 610031, China. sccdlzh@163.com
authors.forename: Zhi-Hao
authors.initials: ZH
authors.lastname: Li
authors.forename: Liang-Ke
authors.initials: LK
authors.lastname: Song
authors.forename: Xiao-Ning
authors.initials: XN
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Shu-Xing
authors.initials: SX
authors.lastname: Wu
authors.forename: Cheng-Long
authors.initials: CL
authors.lastname: Yu
authors.forename: Yu-Ying
authors.initials: YY
authors.lastname: Zong
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002867:Chromones / Q000032:analysis* / Q000737:chemistry; D031180:Cirsium / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012995:Solubility; D013045:Species Specificity
chemical_list: D002867:Chromones; D004365:Drugs, Chinese Herbal; C052786:pectolinarin