title: Functionalization of optical nanotip arrays with an electrochemical microcantilever for multiplexed DNA detection.
abstract: Optical nanotip arrays fabricated on etched fiber bundles were functionalized with DNA spots. Such unconventional substrates (3D and non-planar) are difficult to pattern with standard microfabrication techniques but, using an electrochemical cantilever, up to 400 spots were electrodeposited on the nanostructured optical surface in 5 min. This approach allows each spot to be addressed individually and multiplexed fluorescence detection is demonstrated. Finally, remote fluorescence detection was performed by imaging through the optical fiber bundle itself after hybridisation with the complementary sequence.
authors.affiliation: Institut des Sciences Moléculaires, UMR 5255 CNRS, Université Bordeaux 1-ENSCBP 16, Avenue Pey-Berland, 33607 Pessac, France.
authors.forename: Emeline
authors.initials: E
authors.lastname: Descamps
authors.forename: Nathalie
authors.initials: N
authors.lastname: Duroure
authors.forename: Frédérique
authors.initials: F
authors.lastname: Deiss
authors.forename: Thierry
authors.initials: T
authors.lastname: Leichlé
authors.forename: Catherine
authors.initials: C
authors.lastname: Adam
authors.forename: Pascal
authors.initials: P
authors.lastname: Mailley
authors.forename: Ali
authors.initials: A
authors.lastname: Aït-Ikhlef
authors.forename: Thierry
authors.initials: T
authors.lastname: Livache
authors.forename: Liviu
authors.initials: L
authors.lastname: Nicu
authors.forename: Neso
authors.initials: N
authors.lastname: Sojic
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004247:DNA / Q000032:analysis*; D004563:Electrochemistry / Q000295:instrumentation*; D004867:Equipment Design; D005336:Fiber Optic Technology / Q000295:instrumentation*; D049329:Nanostructures / Q000737:chemistry; D009693:Nucleic Acid Hybridization
chemical_list: D004247:DNA