title: Crystallization and preliminary X-ray crystallographic analysis of the rhesus macaque MHC class I molecule Mamu-B*17 complexed with an immunodominant SIVmac239 Env epitope.
abstract: Long-term nonprogression during simian immunodeficiency virus (SIV) infection has been strongly associated with the major histocompatibility complex (MHC) class I allele Mamu-B*17. Here, a complex of rhesus macaque Mamu-B*17 with rhesus macaque β2-microglobulin (β2m) and an immunodominant peptide (SIVmac239 Env241-251; LRCNDTNYSGF; Env LF11) derived from the SIV Env protein was crystallized by the hanging-drop method using PEG 3350 as a precipitating agent. The crystals belonged to the primitive monoclinic space group P2, with unit-cell parameters a = 68.3, b = 45.0, c = 81.5 Å, β = 96.5°. Assuming the presence of one molecule in the asymmetric unit, the Matthews coefficient and solvent content were calculated to be 2.96 Å(3) Da(-1) and 58.5%, respectively.
authors.affiliation: School of Life Sciences, Sichuan University, Chengdu 610064, People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Jinku
authors.initials: J
authors.lastname: Bao
mesh_terms: D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D015395:Histocompatibility Antigens Class I / Q000032:analysis* / Q000737:chemistry*; D016056:Immunodominant Epitopes / Q000032:analysis* / Q000737:chemistry*; D008253:Macaca mulatta
chemical_list: D015395:Histocompatibility Antigens Class I; D016056:Immunodominant Epitopes; C092249:Mamu-B 01 antigen, Macaca mulatta
keywords: MHC; SIV; elite controllers; rhesus macaque