title: Biochemical study of the effects of cilostazol in rats subjected to acute ischemia and reperfusion of hind limbs.
abstract: OBJECTIVE
  To investigate whether cilostazol has a protective effect on acute ischemia and reperfusion of hind limbs of rats through study of biochemical variables in blood and urine.
  
  
  METHODS
  Forty six animals were randomized and divided into two groups. Group I received a solution of cilostazol (10 mg/Kg) and group II received saline solution 0.9% (SS) by orogastric tube after ligature of the abdominal aorta. After four hours of ischemia the animals were divided into four subgroups: group IA (Cilostazol): two hours of reperfusion. Group IIA (SS): two hours of reperfusion. Group IB (Cilostazol): six hours of reperfusion. Group IIB (SS) six hours of reperfusion. After the reperfusion period, was held to collect urine and blood for biochemical measurements. The biochemical parameters studied were: urea, creatinine, sodium, potassium and myoglobin in blood and urea, creatinine, myoglobin in urine.
  
  
  RESULTS
  There was no statistically significant difference between groups.
  
  
  CONCLUSIONS
  Cilostazol had no protective effect on ischemic acute reperfusion of hind limbs of rats in this model.
authors.affiliation: Center for Technological Research, Mogi das Cruzes University, Mogi das Cruzes, São Paulo, Brazil. sylvio.vascular@uol.com.br
authors.forename: Sylvio Sebastião de
authors.initials: SS
authors.lastname: Souza Júnior
authors.forename: Antonio Augusto
authors.initials: AA
authors.lastname: Moreira Neto
authors.forename: Aurelino Fernandes
authors.initials: AF
authors.lastname: Schmidt Júnior
authors.forename: José Benedito Dias
authors.initials: JB
authors.lastname: Lemos
authors.forename: Olavo Ribeiro
authors.initials: OR
authors.lastname: Rodrigues
mesh_terms: D000818:Animals; D000077407:Cilostazol; D003404:Creatinine / Q000097:blood / Q000652:urine; D004195:Disease Models, Animal; D006614:Hindlimb / Q000098:blood supply*; D008297:Male; D009211:Myoglobin / Q000097:blood; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000188:drug therapy*; D015203:Reproducibility of Results; D013777:Tetrazoles / Q000494:pharmacology*; D013997:Time Factors; D014508:Urea / Q000097:blood / Q000652:urine
chemical_list: D009211:Myoglobin; D010975:Platelet Aggregation Inhibitors; D013777:Tetrazoles; D014508:Urea; D003404:Creatinine; D000077407:Cilostazol