title: Multiparameter flow cytometry: advances in high resolution analysis.
abstract: Over the past 40 years, flow cytometry has emerged as a leading, application-rich technology that supports high-resolution characterization of individual cells which function in complex cellular networks such as the immune system. This brief overview highlights advances in multiparameter flow cytometric technologies and reagent applications for characterization and functional analysis of cells modulating the immune network. These advances significantly support high-throughput and high-content analyses and enable an integrated understanding of the cellular and molecular interactions that underlie complex biological systems.
authors.affiliation: Research and Development, BD Biosciences, La Jolla, CA 92037, USA.
authors.forename: Erika A
authors.initials: EA
authors.lastname: O'Donnell
authors.forename: David N
authors.initials: DN
authors.lastname: Ernst
authors.forename: Ravi
authors.initials: R
authors.lastname: Hingorani
keywords: Antibodies; Fluorescent dyes; Immunophenotyping; Multi-color flow cytometry; Multiplex immunoassays; Single cell analysis/methods