title: CLARITY for mapping the nervous system.
abstract: With potential relevance for brain-mapping work, hydrogel-based structures can now be built from within biological tissue to allow subsequent removal of lipids without mechanical disassembly of the tissue. This process creates a tissue-hydrogel hybrid that is physically stable, that preserves fine structure, proteins and nucleic acids, and that is permeable to both visible-spectrum photons and exogenous macromolecules. Here we highlight relevant challenges and opportunities of this approach, especially with regard to integration with complementary methodologies for brain-mapping studies.
authors.affiliation: Department of Bioengineering, Stanford University, Stanford, California, USA. khchung@mit.edu
authors.forename: Kwanghun
authors.initials: K
authors.lastname: Chung
authors.forename: Karl
authors.initials: K
authors.lastname: Deisseroth
mesh_terms: D000818:Animals; D001931:Brain Mapping / Q000379:methods*; D003952:Diagnostic Imaging / Q000379:methods*; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D008853:Microscopy; D013569:Synapses / Q000502:physiology
chemical_list: D020136:Hydrogel, Polyethylene Glycol Dimethacrylate