title: Association of the -262C/T polymorphism in the catalase gene promoter and the C242T polymorphism of the NADPH oxidase P22phox gene with essential arterial hypertension in patients with diabetes mellitus type 2.
abstract: OBJECTIVE
  The aim of the present study was to test the association between genetic polymorphisms with functional effects on redox regulation: the -262C/T of the catalase gene promoter (rs1001179), the C242T of the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase P22phox gene (rs4673), and the 594C/T polymorphism of the glutathione peroxidase gene (rs1050450) and arterial hypertension (AH) in patients with type 2 diabetes.
  
  
  METHODS
  810 Slovenian subjects (Caucasians) with type 2 diabetes were enrolled in the cross-sectional study. Genotypes were determined by real-time PCR.
  
  
  RESULTS
  Univariate analysis failed to demonstrate an association between either the -262C/T of the catalase gene promoter (rs1001179) or the C242T polymorphism of the P22phox gene (rs4673) or the 594C/T polymorphism of the glutathione peroxidase gene (rs1050450) and AH. After adjustment for age, body mass index, fibrinogen level and high sensitivity C-reactive protein level, rs4673 was found to be an independent risk factor for AH in subjects with type 2 diabetes, whereas rs1001179 and rs1050450 were not.
  
  
  CONCLUSIONS
  According to the results of cross-sectional study, the tested polymorphism of the NADPH oxidase P22phox gene (rs4673) was found to be associated with the development of AH, indicating that the oxidative stress gene NADPH oxidase might be implicated in the pathogenesis of AH in subjects with type 2 diabetes.
authors.affiliation: Medical Faculty Ljubljana, University Ljubljana , Ljubljana , Slovenia.
authors.forename: Daniel
authors.initials: D
authors.lastname: Petrovič
mesh_terms: D000368:Aged; D002097:C-Reactive Protein / Q000378:metabolism; D016022:Case-Control Studies; D002374:Catalase / Q000235:genetics*; D003430:Cross-Sectional Studies; D003924:Diabetes Mellitus, Type 2 / Q000150:complications* / Q000201:enzymology / Q000235:genetics*; D005260:Female; D005340:Fibrinogen / Q000378:metabolism; D020022:Genetic Predisposition to Disease; D005979:Glutathione Peroxidase / Q000235:genetics; D006801:Humans; D006973:Hypertension / Q000150:complications* / Q000201:enzymology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D019255:NADPH Oxidases / Q000235:genetics*; D018384:Oxidative Stress / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D012307:Risk Factors; D017524:Slovenia
chemical_list: D005340:Fibrinogen; D002097:C-Reactive Protein; C577219:CAT protein, human; D002374:Catalase; D005979:Glutathione Peroxidase; D019255:NADPH Oxidases; C086986:CYBA protein, human