title: Germ-line-competent embryonic stem cells of the Chinese Kunming mouse strain with long-term self-renewal ability.
abstract: Kunming (KM) mice are the most widely used strain in China. However, authentic embryonic stem cells (ESCs) from KM mice have never been available, and this hampers the genetic manipulation of this valuable mice strain. In this study, we show that KM ESCs can be efficiently derived and maintained in chemically defined N2B27 medium with the presence of two small molecules PD0325901 and CHIR99021 (2i medium). These KM ESCs exhibit all features of ESCs, including long-term self-renewal ability, expression of key molecular markers (Oct4, Nanog, and Sox2), the ability to form teratomas, and the capacity to incorporate into the developing embryo and then transmit through the germ line.
authors.affiliation: Laboratory of Viral and Gene Therapy, Eastern Hepatobiliary Surgical Hospital, The Second Military Medical University, Shanghai, China.
authors.forename: Xinrong
authors.initials: X
authors.lastname: Peng
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.forename: Baotian
authors.initials: B
authors.lastname: Yang
authors.forename: Chuanyin
authors.initials: C
authors.lastname: Shi
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.forename: Lihua
authors.initials: L
authors.lastname: Jiang
authors.forename: Huajun
authors.initials: H
authors.lastname: Jin
authors.forename: Linfang
authors.initials: L
authors.lastname: Li
authors.forename: Haili
authors.initials: H
authors.lastname: Zhu
authors.forename: Mengchao
authors.initials: M
authors.lastname: Wu
authors.forename: Qijun
authors.initials: Q
authors.lastname: Qian
mesh_terms: D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D002681:China; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D004159:Diphenylamine / Q000031:analogs & derivatives / Q000494:pharmacology; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D005260:Female; D005854:Germ Cells / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D018398:Homeodomain Proteins / Q000378:metabolism; D066298:In Vitro Techniques; D051379:Mice; D008815:Mice, Inbred Strains; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D011247:Pregnancy; D011725:Pyridines / Q000494:pharmacology; D011743:Pyrimidines / Q000494:pharmacology; D055748:SOXB1 Transcription Factors / Q000378:metabolism; D013997:Time Factors
chemical_list: D001549:Benzamides; D015415:Biomarkers; C473711:Chir 99021; D016895:Culture Media, Serum-Free; D018398:Homeodomain Proteins; D000071317:Nanog Homeobox Protein; C475540:Nanog protein, mouse; D050814:Octamer Transcription Factor-3; C494174:Pou5f1 protein, mouse; D011725:Pyridines; D011743:Pyrimidines; D055748:SOXB1 Transcription Factors; C527783:Sox2 protein, mouse; C506614:mirdametinib; D004159:Diphenylamine