title: A global S. cerevisiae small ubiquitin-related modifier (SUMO) system interactome.
abstract: The small ubiquitin-related modifier (SUMO) system has been implicated in a number of biological functions, yet the individual components of the SUMO machinery involved in each of these activities were largely unknown. Here we report the first global SUMO system interactome. Using affinity purification coupled with mass spectrometry, we identify >450 protein-protein interactions surrounding the SUMO E2, Siz type E3s and SUMO-specific proteases in budding yeast. Exploiting this information-rich resource, we validate several Siz1- and Siz2-specific substrates, identify a nucleoporin required for proper Ulp1 localization, and uncover important new roles for Ubc9 and Ulp2 in the maintenance of ribosomal DNA.
authors.affiliation: Ontario Cancer Institute, University Health Network and Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada.
authors.forename: Tharan
authors.initials: T
authors.lastname: Srikumar
authors.forename: Megan C
authors.initials: MC
authors.lastname: Lewicki
authors.forename: Brian
authors.initials: B
authors.lastname: Raught
mesh_terms: D002453:Cell Cycle / Q000235:genetics; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism; D004275:DNA, Ribosomal / Q000235:genetics; D010450:Endopeptidases / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D028861:Nuclear Pore Complex Proteins / Q000235:genetics / Q000378:metabolism; D025941:Protein Interaction Mapping; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D025841:Small Ubiquitin-Related Modifier Proteins / Q000235:genetics* / Q000378:metabolism; D059467:Transcriptome; D025801:Ubiquitin / Q000235:genetics / Q000378:metabolism; D044763:Ubiquitin-Conjugating Enzymes / Q000235:genetics / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism; D000098843:Ubiquitin-Conjugating Enzyme UBC9
chemical_list: D004275:DNA, Ribosomal; D028861:Nuclear Pore Complex Proteins; D029701:Saccharomyces cerevisiae Proteins; C436836:Siz2 protein, S cerevisiae; D025841:Small Ubiquitin-Related Modifier Proteins; D025801:Ubiquitin; D044763:Ubiquitin-Conjugating Enzymes; D044767:Ubiquitin-Protein Ligases; D010450:Endopeptidases; C405949:ULP2 protein, S cerevisiae; D003546:Cysteine Endopeptidases; C118083:Ulp1 protease; C436837:Siz1 protein, S cerevisiae; D000098843:Ubiquitin-Conjugating Enzyme UBC9