title: Effects of post-thaw incubation on motility, acrosomal integrity and in vitro fertilizing capacity of boar spermatozoa cryopreserved in 0.5 and 5ml straws.
abstract: The effect of post-thaw incubation (0 vs. 5h at 15°C) and straw size (5 vs. 0.5ml) on motility, acrosomal integrity and in vitro fertilizing (IVF) capacity of cryopreserved boar spermatozoa was studied. In samples assessed immediately after thawing, no differences were found between the two straw sizes. After 5h post-thaw incubation, all parameters, except polyspermy, decreased and, spermatozoa packaged in 5ml straws showed better functional and IVF parameters than these in 0.5ml straws.
authors.affiliation: Department of Agricultural and Animal Production, Metropolitan Autonomous University, Mexico.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Córdova
authors.forename: Rocío
authors.initials: R
authors.lastname: Hernández-Gil
authors.forename: Cristian Alejandro
authors.initials: CA
authors.lastname: Córdova-Jiménez
authors.forename: Diogo José
authors.initials: DJ
authors.lastname: Cardilli
authors.forename: Kellen
authors.initials: K
authors.lastname: de Sousa Oliveira
authors.forename: José Félix
authors.initials: JF
authors.lastname: Pérez-Gutiérrez
mesh_terms: D000177:Acrosome / Q000502:physiology*; D000818:Animals; D015925:Cryopreservation / Q000295:instrumentation / Q000662:veterinary*; D005307:Fertilization in Vitro / Q000662:veterinary*; D008297:Male; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility / Q000502:physiology*; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology*; D034421:Sus scrofa / Q000502:physiology*; D016896:Treatment Outcome