title: Isolation of circulating microRNA in saliva.
abstract: Oral cancer diagnosis can be greatly facilitated by early diagnosis in order to improve the 50 % 5-year mortality that has not changed much over the last years. Saliva is an easily accessible medium that has been shown to contain microvesicles (exosomes) that enclose microRNAs. We have previously demonstrated that the majority of salivary microRNAs are within exosomes. MicroRNAs have been implicated in oral cancer and the use of salivary exosomal microRNAs holds the promise of identification of diagnostic and prognostic markers.
authors.affiliation: Sjögren's Syndrome Clinic, Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA.
authors.forename: Alessia
authors.initials: A
authors.lastname: Gallo
authors.forename: Ilias
authors.initials: I
authors.lastname: Alevizos
mesh_terms: D014408:Biomarkers, Tumor / Q000302:isolation & purification*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D019343:Citric Acid / Q000494:pharmacology; D042241:Early Diagnosis; D055354:Exosomes / Q000737:chemistry* / Q000648:ultrastructure; D006801:Humans; D035683:MicroRNAs / Q000302:isolation & purification*; D008854:Microscopy, Electron; D009062:Mouth Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D010306:Parotid Gland / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D060888:Real-Time Polymerase Chain Reaction; D012463:Saliva / Q000737:chemistry*; D013048:Specimen Handling; D013997:Time Factors
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; D019343:Citric Acid