title: RICE SALT SENSITIVE3 forms a ternary complex with JAZ and class-C bHLH factors and regulates jasmonate-induced gene expression and root cell elongation.
abstract: Plasticity of root growth in response to environmental cues and stresses is a fundamental characteristic of land plants. However, the molecular basis underlying the regulation of root growth under stressful conditions is poorly understood. Here, we report that a rice nuclear factor, RICE SALT SENSITIVE3 (RSS3), regulates root cell elongation during adaptation to salinity. Loss of function of RSS3 only moderately inhibits cell elongation under normal conditions, but it provokes spontaneous root cell swelling, accompanied by severe root growth inhibition, under saline conditions. RSS3 is preferentially expressed in the root tip and forms a ternary complex with class-C basic helix-loop-helix (bHLH) transcription factors and JASMONATE ZIM-DOMAIN proteins, the latter of which are the key regulators of jasmonate (JA) signaling. The mutated protein arising from the rss3 allele fails to interact with bHLH factors, and the expression of a significant portion of JA-responsive genes is upregulated in rss3. These results, together with the known roles of JAs in root growth regulation, suggest that RSS3 modulates the expression of JA-responsive genes and plays a crucial role in a mechanism that sustains root cell elongation at appropriate rates under stressful conditions.
authors.affiliation: Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Toda
authors.forename: Maiko
authors.initials: M
authors.lastname: Tanaka
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ogawa
authors.forename: Kyo
authors.initials: K
authors.lastname: Kurata
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Kurotani
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Habu
authors.forename: Tsuyu
authors.initials: T
authors.lastname: Ando
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Sugimoto
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Mitsuda
authors.forename: Etsuko
authors.initials: E
authors.lastname: Katoh
authors.forename: Kiyomi
authors.initials: K
authors.lastname: Abe
authors.forename: Akio
authors.initials: A
authors.lastname: Miyao
authors.forename: Hirohiko
authors.initials: H
authors.lastname: Hirochika
authors.forename: Tsukaho
authors.initials: T
authors.lastname: Hattori
authors.forename: Shin
authors.initials: S
authors.lastname: Takeda
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics; D000595:Amino Acid Sequence; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics* / Q000378:metabolism; D003517:Cyclopentanes / Q000494:pharmacology*; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D009154:Mutation; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D054883:Oxylipins / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018517:Plant Roots / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D011485:Protein Binding; D020133:Reverse Transcriptase Polymerase Chain Reaction; D054712:Salinity; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride / Q000494:pharmacology; D020798:Two-Hybrid System Techniques
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D012965:Sodium Chloride; C011006:jasmonic acid