title: Detection of ascitic feline coronavirus RNA from cats with clinically suspected feline infectious peritonitis.
abstract: Ascitic feline coronavirus (FCoV) RNA was examined in 854 cats with suspected feline infectious peritonitis (FIP) by RT-PCR. The positivity was significantly higher in purebreds (62.2%) than in crossbreds (34.8%) (P<0.0001). Among purebreds, the positivities in the Norwegian forest cat (92.3%) and Scottish fold (77.6%) were significantly higher than the average of purebreds (P=0.0274 and 0.0251, respectively). The positivity was significantly higher in males (51.5%) than in females (35.7%) (P<0.0001), whereas no gender difference has generally been noted in FCoV antibody prevalence, indicating that FIP more frequently develops in males among FCoV-infected cats. Genotyping was performed for 377 gene-positive specimens. Type I (83.3%) was far more predominantly detected than type II (10.6%) (P<0.0001), similar to previous serological and genetic surveys.
authors.affiliation: Veterinary Diagnostic Laboratory, Marupi Lifetech Co., Ltd., 103 Fushiocho, Ikeda, Osaka 563-0011, Japan.
authors.forename: Takehisa
authors.initials: T
authors.lastname: Soma
authors.forename: Makoto
authors.initials: M
authors.lastname: Wada
authors.forename: Satoshi
authors.initials: S
authors.lastname: Taharaguchi
authors.forename: Tomoko
authors.initials: T
authors.lastname: Tajima
mesh_terms: D000367:Age Factors; D000818:Animals; D001201:Ascites / Q000821:virology*; D002415:Cats; D016765:Coronavirus, Feline / Q000235:genetics / Q000302:isolation & purification*; D016766:Feline Infectious Peritonitis / Q000453:epidemiology / Q000235:genetics* / Q000821:virology; D005260:Female; D005838:Genotype; D007564:Japan / Q000453:epidemiology; D008297:Male; D015995:Prevalence; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012737:Sex Factors
chemical_list: D012367:RNA, Viral