title: LARP1 specifically recognizes the 3' terminus of poly(A) mRNA.
abstract: A poly(A) tail functions in mRNA turnover and in facilitating translation as a ribonucleoprotein complex with poly(A) binding proteins (PABPs). However, factors that associate with the poly(A) tail other than PABPs have not been described. Using proteomics, we identified candidate proteins that interact to the 3' terminus of the poly(A) tail. Among these proteins, we focused on La motif-related protein 1 (LARP1) and found that LARP1 specifically recognizes the 3' termini of normal poly(A) tails. We also reveal that LARP1 stabilizes multiple mRNAs carrying 5' terminal oligopyrimidine tract (5'TOP). Our findings suggest that LARP1 may be involved in the post-transcriptional regulation of gene expression, at least in several 5'TOP mRNAs, through the binding to 3' terminus of the poly(A) tail.
authors.affiliation: Molecular Profiling Research Center for Drug Discovery(molprof), National Institute of Advanced Industrial Science and Technology (AIST), 2-4-7 Aomi, Koutou, Tokyo 135-0064, Japan.
authors.forename: Kazuma
authors.initials: K
authors.lastname: Aoki
authors.forename: Shungo
authors.initials: S
authors.lastname: Adachi
authors.forename: Masae
authors.initials: M
authors.lastname: Homoto
authors.forename: Hideo
authors.initials: H
authors.lastname: Kusano
authors.forename: Katsuyuki
authors.initials: K
authors.lastname: Koike
authors.forename: Tohru
authors.initials: T
authors.lastname: Natsume
mesh_terms: D001324:Autoantigens / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D015870:Gene Expression; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D057809:HEK293 Cells; D006801:Humans; D008969:Molecular Sequence Data; D011061:Poly A / Q000378:metabolism*; D039122:Poly(A)-Binding Protein I / Q000378:metabolism; D011485:Protein Binding; D020543:Proteome / Q000378:metabolism; D020871:RNA Stability; D012333:RNA, Messenger / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000235:genetics; D012261:Ribonucleoproteins / Q000235:genetics / Q000378:metabolism*; D000097585:SS-B Antigen
chemical_list: D001324:Autoantigens; D011061:Poly A; D039122:Poly(A)-Binding Protein I; D020543:Proteome; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D012261:Ribonucleoproteins; D000097585:SS-B Antigen; C026452:messenger ribonucleoprotein