title: [HPLC fingerprint chromatogram of Polygonum multiflorum from Guizhou].
abstract: OBJECTIVE
  To establish the fingerprint of Polygonum multiflorum from Guizhou and provide a standard for its quality control.
  
  
  METHODS
  HPLC analysis was performed on Agillent ZABAX-C18 (4.6 mm x 250 mm, 5 microm), gradient eluted composed of acetonitrile-0.4% water solution of phosphoric acid. Column temperature was set at 25 degrees C and the flow rate was 1 mL/min. The detection wavelength was 280 nm and the analysis time was 60 min.
  
  
  RESULTS
  9 common peaks were identified. The RSD of the relative retention time and the relative peak area were less than 3% in analyzing its precision, stability and repeatability of the common peaks, and the similarity of the 16 batches of sample was more than 0.9.
  
  
  CONCLUSIONS
  The method is simple and reliable, and it can provide a standard and guidance for quality control of Polygonum multiflorum.
authors.affiliation: Guizhou Institute of Technology, Guiyang 550003, China. 352897303@qq.com
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Hui-Juan
authors.initials: HJ
authors.lastname: Wang
authors.forename: Bing
authors.initials: B
authors.lastname: Lin
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhao
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D002681:China; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000592:standards; D018517:Plant Roots / Q000737:chemistry / Q000254:growth & development; D010946:Plants, Medicinal / Q000737:chemistry* / Q000254:growth & development; D028502:Polygonum / Q000737:chemistry* / Q000254:growth & development; D011786:Quality Control; D015203:Reproducibility of Results
chemical_list: D004365:Drugs, Chinese Herbal