title: Identification and functional characteristics of chlorpyrifos-degrading and plant growth promoting bacterium Acinetobacter calcoaceticus.
abstract: A bacterial strain D10 with strong ability of degrading chlorpyrifos was isolated from rhizosphere of chives contaminated with pesticide. It was found that it's capable of utilizing chlorpyrifos as the sole source of carbon for growth, and within the first 4 days the extent of degradation at initial concentration of 100 mg L(-1) was 60.0%. It also showed a high ability of degrading chlorpyrifos in sterilized soil, and the degradation reached up to 60.2% after 18 days. In addition, the strain D10 also showed multiple plant growth-promoting traits of phosphate solubilization, indole-3-acetic acid and siderophore production. The results indicate that the strain D10 has potential in the application of pesticide-degrading and plant growth promotion. Strain D10 was identified as Acinetobacter calcoaceticus based on its morphological, physiological-biochemical properties and the 16S rRNA sequence analysis.
authors.affiliation: College of Life Science, Shandong Normal University, Jinan, Shandong, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.forename: Jiao
authors.initials: J
authors.lastname: Zhao
mesh_terms: D016954:Acinetobacter calcoaceticus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D015373:Bacterial Typing Techniques; D001711:Biotransformation; D002244:Carbon / Q000378:metabolism; D004390:Chlorpyrifos / Q000378:metabolism*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D007210:Indoleacetic Acids / Q000378:metabolism; D008969:Molecular Sequence Data; D010710:Phosphates / Q000378:metabolism; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D017262:Siderophores / Q000378:metabolism; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D007210:Indoleacetic Acids; D010710:Phosphates; D010937:Plant Growth Regulators; D012336:RNA, Ribosomal, 16S; D017262:Siderophores; C030737:indoleacetic acid; D002244:Carbon; D004390:Chlorpyrifos
keywords: Acinetobacter calcoaceticus; Chlorpyrifos; Identification; Plant growth promotion; Soil bioremediation