title: Intrathecal injection of P/Q type voltage-gated calcium channel antibodies from paraneoplastic cerebellar degeneration cause ataxia in mice.
abstract: The role of antibodies against the P/Q type voltage-gated calcium channels (VGCC-ab) in the pathogenesis of paraneoplastic cerebellar degeneration (PCD) and lung cancer is unclear. We evaluated in mice the effect of intrathecal injection of IgG purified from serum of a patient with both PCD and Lambert-Eaton myasthenic syndrome (LEMS), and from another patient with isolated LEMS. Mice injected with PCD/LEMS IgG developed marked, reversible ataxia compared with those injected with LEMS or control IgG. These findings suggest that P/Q-type VGCC-ab may play a role in the pathogenesis of ataxia in patients with PCD and SCLC.
authors.affiliation: Laboratori de Neurofarmacologia, Facultat de Ciències de la Salut i de la Vida, Universitat Pompeu Fabra, Barcelona, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Martín-García
authors.forename: Francesco
authors.initials: F
authors.lastname: Mannara
authors.forename: Javier
authors.initials: J
authors.lastname: Gutiérrez-Cuesta
authors.forename: Lidia
authors.initials: L
authors.lastname: Sabater
authors.forename: Josep
authors.initials: J
authors.lastname: Dalmau
authors.forename: Rafael
authors.initials: R
authors.lastname: Maldonado
authors.forename: Francesc
authors.initials: F
authors.lastname: Graus
mesh_terms: D000818:Animals; D001323:Autoantibodies / Q000633:toxicity*; D020865:Calcium Channels, P-Type / Q000097:blood / Q000276:immunology*; D020907:Calcium Channels, Q-Type / Q000276:immunology*; D002524:Cerebellar Ataxia / Q000139:chemically induced / Q000276:immunology*; D057809:HEK293 Cells; D006801:Humans; D007278:Injections, Spinal; D051379:Mice; D020362:Paraneoplastic Cerebellar Degeneration / Q000276:immunology*; D051381:Rats
chemical_list: D001323:Autoantibodies; D020865:Calcium Channels, P-Type; D020907:Calcium Channels, Q-Type
keywords: Intrathecal injection; Lambert–Eaton Myasthenic syndrome; Mouse model; P/Q-type voltage-gated calcium channel antibodies; Paraneoplastic cerebellar degeneration; Small-cell lung cancer