title: Effect of the structural features of hydrochloric acid-deamidated wheat gluten on its susceptibility to enzymatic hydrolysis.
abstract: The effect of the structural features of hydrochloric acid-deamidated wheat gluten with different degrees of deamidation (DDs) on the susceptibility to enzymatic hydrolysis by pancreatin was investigated. The wheat gluten deamidated by hydrochloric acid with a DD of 55% revealed the highest susceptibility to enzymatic hydrolysis as evaluated by the hydrolysis degree and nitrogen solubility index of the hydrolysates. An increase of peptides with MW below 3000 Da was observed as the DD increased. Raman spectra in the 1740-1800 cm⁻¹ and 521-530 cm⁻¹ range suggested that wheat gluten had taken off the deamidation with different DDs and that the disulfide bond had disrupted the sulfhydryl groups with different intensities, respectively. Results from the deconvolution of the amide I region of FTIR spectra in the 1600-1700 cm⁻¹ range showed that the content of the α-helix decreased and that the content of the β-turn and β-sheet increased with increasing DDs, which improved the molecular structure and flexibility of wheat gluten. A scanning electron microscope (SEM) revealed that the image of HDG-55% presented the smoothest surface and the least uniform pore, enabling the sample to be more susceptible to enzymatic hydrolysis. The above information will enable us to better understand the effect of structure on the susceptibility of deamidated wheat gluten.
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology , Guangzhou 510640, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Cui
authors.forename: Qingling
authors.initials: Q
authors.lastname: Hu
authors.forename: Jiaoyan
authors.initials: J
authors.lastname: Ren
authors.forename: Haifeng
authors.initials: H
authors.lastname: Zhao
authors.forename: Lijun
authors.initials: L
authors.lastname: You
authors.forename: Mouming
authors.initials: M
authors.lastname: Zhao
mesh_terms: D000818:Animals; D002681:China; D004044:Dietary Proteins / Q000737:chemistry* / Q000378:metabolism; D005524:Food Technology; D005983:Glutens / Q000737:chemistry* / Q000378:metabolism*; D006851:Hydrochloric Acid / Q000737:chemistry; D007202:Indicators and Reagents / Q000737:chemistry; D010194:Pancreatin / Q000378:metabolism; D010447:Peptide Hydrolases / Q000378:metabolism*; D011487:Protein Conformation; D055550:Protein Stability; D059748:Proteolysis; D012639:Seeds / Q000737:chemistry*; D013381:Subtilisins / Q000378:metabolism; D013499:Surface Properties; D014908:Triticum / Q000737:chemistry*
chemical_list: D004044:Dietary Proteins; D007202:Indicators and Reagents; D005983:Glutens; D010194:Pancreatin; D010447:Peptide Hydrolases; D013381:Subtilisins; D006851:Hydrochloric Acid