title: [scpA the new salicylate hydroxylase gene localized on salicylate/caprolactam degradation plasmids].
abstract: Both caprolactam and salicylate biodegradation by Pseudomonas salicylate/caprolactam degraders is controlled by large conjugative plasmids (SAL/CAP). Some of these plasmids determined to be the members of IncP-7 group. The new salicylate 1-hydroxylase gene (scpA) on SAL/CAP-plasmids has been detected and partially sequenced. Gene scpA was equally related to closest homologs nahG (NAH7), salA (P. reinekei MT1) and nahU (pND6-1), but identity of scpA to these genes did not exceed 72-74%. Synthesis of salicylate 1-hydroxylase ScpA was not induced by salicylate. This enzyme had wide substrate specificity and exhibited highest specific activity with 4-methylsalicylate and nonsubstituted salicylate. Besides pseudomonad's salicylate degradative conjugative plasmids without "classical" nah2-operon and harboring only salicylate 1-hydroxylase gene nahU have been firstly described.
authors.forename: A V
authors.initials: AV
authors.lastname: Panov
authors.forename: O V
authors.initials: OV
authors.lastname: Volkova
authors.forename: I F
authors.initials: IF
authors.lastname: Puntus
authors.forename: T Z
authors.initials: TZ
authors.lastname: Esikova
authors.forename: I A
authors.initials: IA
authors.lastname: Kosheleva
authors.forename: A M
authors.initials: AM
authors.lastname: Boronin
mesh_terms: D002209:Caprolactam / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D010957:Plasmids; D016958:Pseudomonas putida / Q000201:enzymology*; D012459:Salicylates / Q000378:metabolism
chemical_list: D012459:Salicylates; D002209:Caprolactam; D006899:Mixed Function Oxygenases; C022619:salicylate 1-monooxygenase