title: Association of single nucleotide polymorphism rs3775291 in the coding region of the TLR3 gene with predisposition to tick-borne encephalitis in a Russian population.
abstract: Tick-borne encephalitis (TBE) is a central nervous system (CNS) disease caused by the neurotropic, positive-sense RNA virus, tick-borne encephalitis virus (TBEV). A possible association between predisposition to TBE in a Russian population and two polymorphisms, a 32bp deletion in the coding region of the chemokine receptor CCR5 gene and the rs3775291 single nucleotide polymorphism (SNP) (G/A, Leu412Phe) in exon 4 of the toll-like receptor TLR3 gene, was investigated. The genotypic and allelic frequencies of these polymorphisms were analyzed in 137 non-immunized TBE patients with different clinical manifestations, including fever (35), meningitis (62), and severe CNS disease (40), as well as in a control population (269 randomly selected Novosibirsk citizens). The frequencies of the TLR3 G allele and G/G homozygotes were significantly higher among the patients with TBE compared with the control group (P=0.029 and 0.037, respectively), especially among patients with severe disease (P=0.018 and 0.017, respectively). These results indicate that the G allele (within the G/G homozygous genotype) of the TLR3 rs3775291 SNP is associated with predisposition to TBE in the Russian population.
authors.affiliation: Institute of Cytology and Genetics, Russian Academy of Sciences, Siberian Branch, 10 Lavrentyeva Ave., Novosibirsk 630090, Russia. barkhash@bionet.nsc.ru
authors.forename: Andrey V
authors.initials: AV
authors.lastname: Barkhash
authors.forename: Mikhail I
authors.initials: MI
authors.lastname: Voevoda
authors.forename: Aida G
authors.initials: AG
authors.lastname: Romaschenko
mesh_terms: D004669:Encephalitis Viruses, Tick-Borne / Q000235:genetics*; D004675:Encephalitis, Tick-Borne / Q000453:epidemiology* / Q000235:genetics*; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D019713:Receptors, CCR5 / Q000235:genetics; D012426:Russia / Q000453:epidemiology; D051196:Toll-Like Receptor 3 / Q000235:genetics*
chemical_list: D019713:Receptors, CCR5; C495344:TLR3 protein, human; D051196:Toll-Like Receptor 3
keywords: Chemokine receptor 5 (CCR5) gene; Human genetic predisposition; Single nucleotide polymorphism; Tick-borne encephalitis (TBE); Toll-like receptor 3 (TLR3) gene