title: Inhibitory effect of Pseudomonas putida nitrogen-related phosphotransferase system on conjugative transfer of IncP-9 plasmid from Escherichia coli.
abstract: Conjugative plasmid transfer systems have been well studied, but very little is known about the recipient factors that control horizontal transmission. A self-transmissible IncP-9 naphthalene catabolic plasmid, NAH7, carries the traF gene, whose product is considered to be a host-range modifier of NAH7, because its traF deletion mutant (NAH7dF) is transmissible from Pseudomonas putida to P. putida and Escherichia coli and from E. coli to E. coli, but not from E. coli to P. putida. In this study, transposon mutagenesis of P. putida KT2440 was performed to isolate the mutants that could receive NAH7dF from E. coli. The mutants had the transposon insertions in ptsP or ptsO, encoding two of three components of the nitrogen-related phosphotransferase system (PTS(Ntr) ). The KT2440 derivative lacking ptsN, encoding the remaining component of PTS(Ntr) , was also able to receive NAH7dF. These results indicated that the PTS(Ntr) in P. putida is involved in inhibition of conjugative transfer of NAH7dF from E. coli. Our further experiments using site-directed mutants suggested the indirect involvement of the phosphorylated form of PtsO in the inhibition of the conjugative transfer. Conjugative transfer of NAH7 and another IncP-9 plasmid, pWW0, from E. coli was partially inhibited by the PtsO function in KT2440.
authors.affiliation: Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Inoue
authors.forename: Ryo
authors.initials: R
authors.lastname: Miyazaki
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Ohtsubo
authors.forename: Yuji
authors.initials: Y
authors.lastname: Nagata
authors.forename: Masataka
authors.initials: M
authors.lastname: Tsuda
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000235:genetics*; D009584:Nitrogen / Q000378:metabolism*; D010766:Phosphorylation; D010770:Phosphotransferases / Q000235:genetics / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics*; D016958:Pseudomonas putida / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D010770:Phosphotransferases; D009584:Nitrogen
keywords: IncP-9 plasmid; PTSN
  tr; Pseudomonas; conjugation; host range