title: An electrochemiluminescence assay for gp340 (DMBT1).
abstract: Gp340 is a member of the scavenger receptor cysteine-rich family of innate immune molecules and also functions as a tumor suppressor. This study describes a picogram-level assay using electrochemiluminescence technology on the MesoScale Discovery platform. Antibodies were evaluated and the best pair was used to assay whole-mouth stimulated saliva and cervical/vaginal lavage. The assay was tested using specimens obtained from healthy volunteers to determine if gp340 concentration in saliva correlates with levels in vaginal lavage fluid. Interestingly, no correlation was determined between gp340 content in these two fluids.
authors.affiliation: Department of Basic Sciences, College of Dentistry, New York University, New York, NY 10010, USA.
authors.forename: Cheryl
authors.initials: C
authors.lastname: Barber
authors.forename: Drew
authors.initials: D
authors.lastname: Weissman
authors.forename: Kurt
authors.initials: K
authors.lastname: Barnhart
authors.forename: Minal
authors.initials: M
authors.lastname: Dalvi
authors.forename: William R
authors.initials: WR
authors.lastname: Abrams
authors.forename: Daniel
authors.initials: D
authors.lastname: Malamud
mesh_terms: D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; D055664:Electrochemical Techniques / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D008297:Male; D011956:Receptors, Cell Surface / Q000032:analysis*; D051116:Receptors, Scavenger / Q000032:analysis*; D012463:Saliva / Q000737:chemistry*; D012680:Sensitivity and Specificity; D025521:Tumor Suppressor Proteins; D044364:Vaginal Douching
chemical_list: D002135:Calcium-Binding Proteins; C108174:DMBT1 protein, human; D004268:DNA-Binding Proteins; D011956:Receptors, Cell Surface; D051116:Receptors, Scavenger; D025521:Tumor Suppressor Proteins
keywords: Cervical vaginal lavage; MesoScale Discovery; Salivary agglutinin; Scavenger receptor cysteine-rich; Whole-mouth stimulated saliva