title: Multiple univariate data analysis reveals the inulin effects on the high-fat-diet induced metabolic alterations in rat myocardium and testicles in the preobesity state.
abstract: Obesity is a worldwide epidemic and a well-known risk factor for many diseases affecting billions of people's health and well-being. However, little information is available for metabolic changes associated with the effects of obesity development and interventions on cardiovascular and reproduction systems. Here, we systematically analyzed the effects of high-fat diet (HFD) and inulin intake on the metabolite compositions of myocardium and testicle using NMR spectroscopy. We developed a useful high-throughput method based on multiple univariate data analysis (MUDA) to visualize and efficiently extract information on metabolites significantly affected by an intervention. We found that HFD caused widespread metabolic changes in both rat myocardium and testicles involving fatty acid β-oxidation together with the metabolisms of choline, amino acids, purines and pyrimidines even before HFD caused significant body-weight increases. Inulin intake ameliorated some of the HFD-induced metabolic changes in both myocardium (3-HB, lactate and guanosine) and testicle tissues (3-HB, inosine and betaine). A remarkable elevation of scyllo-inositol was also observable with inulin intake in both tissues. These findings offered essential information for the inulin effects on the HFD-induced metabolic changes and demonstrated this MUDA method as a powerful alternative to traditionally used multivariate data analysis for metabonomics.
authors.affiliation: Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics-Hubei Bioinformatics and Molecular Imaging Key Laboratory, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, P. R. China.
authors.forename: Yixuan
authors.initials: Y
authors.lastname: Duan
authors.forename: Yanpeng
authors.initials: Y
authors.lastname: An
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Bifeng
authors.initials: B
authors.lastname: Liu
authors.forename: Yulan
authors.initials: Y
authors.lastname: Wang
authors.forename: Huiru
authors.initials: H
authors.lastname: Tang
mesh_terms: D000818:Animals; D059305:Diet, High-Fat; D006321:Heart / Q000187:drug effects*; D006801:Humans; D007444:Inulin / Q000008:administration & dosage*; D008297:Male; D023421:Models, Animal; D009206:Myocardium / Q000378:metabolism; D009765:Obesity / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D013737:Testis / Q000187:drug effects* / Q000378:metabolism
chemical_list: D007444:Inulin