title: Development of UV-excitable red and near-infrared fluorescent labels and their application for simultaneous multicolor bioimaging by single-wavelength excitation.
abstract: We report a new type of UV-excitable red/NIR-emissive fluorescent dyads (PKF series). Conjugation of a pyrene and a novel bright red/near-infrared (NIR) fluorophore resulted in large quasi-Stokes shift while retaining intense fluorescence emission and sharp spectral bands. Labeling of PKF dyads to biomolecules was performed by means of introduction of a succinimidyl ester. Simultaneous Ca(2+)/albumin dual-color intracellular imaging by PKF in combination with fura-2 (UV-excitable/VIS-emissive Ca(2+) indicator) reveals its usefulness as a new bioimaging tool.
authors.affiliation: Department of Applied Chemistry, Faculty of Science and Technology, Keio University, Kohoku-ku, Yokohama, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Mizuno
authors.forename: Keitaro
authors.initials: K
authors.lastname: Umezawa
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Shindo
authors.forename: Daniel
authors.initials: D
authors.lastname: Citterio
authors.forename: Kotaro
authors.initials: K
authors.lastname: Oka
authors.forename: Koji
authors.initials: K
authors.lastname: Suzuki
mesh_terms: D000418:Albumins / Q000737:chemistry*; D002118:Calcium / Q000737:chemistry*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D016257:Fura-2 / Q000737:chemistry*; D015394:Molecular Structure; D011721:Pyrenes / Q000737:chemistry; D014466:Ultraviolet Rays
chemical_list: D000418:Albumins; D005456:Fluorescent Dyes; D011721:Pyrenes; C030984:pyrene; D002118:Calcium; D016257:Fura-2