title: Genotoxicity induced by Taenia solium and its reduction by immunization with calreticulin in a hamster model of taeniosis.
abstract: Genotoxicity induced by neurocysticercosis has been demonstrated in vitro and in vivo in humans. The adult stage of Taenia solium lodges in the small intestine and is the main risk factor to acquire neurocysticercosis, nevertheless its carcinogenic potential has not been evaluated. In this study, we determined the genotoxic effect of T. solium infection in the hamster model of taeniosis. In addition, we assessed the effect of oral immunization with recombinant T. solium calreticulin (rTsCRT) plus cholera toxin as adjuvant on micronuclei induction, as this protein has been shown to induce 33-44% protection in the hamster model of taeniosis. Blood samples were collected from the orbital venous plexus of noninfected and infected hamsters at different days postinfection, as well as from orally immunized animals, to evaluate the frequency of micronucleated reticulocytes as a measure of genotoxicity induced by parasite exposure and rTsCRT vaccination. Our results indicate that infection with T. solium caused time-dependent DNA damage in vivo and that rTsCRT immunization reduced the genotoxic damage induced by the presence of the tapeworms.
authors.affiliation: Departamento de Medicina Genómica y Toxicología Ambiental, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México UNAM, México, D.F., México.
authors.forename: Ana María
authors.initials: AM
authors.lastname: Salazar
authors.forename: Fela
authors.initials: F
authors.lastname: Mendlovic
authors.forename: Mayra
authors.initials: M
authors.lastname: Cruz-Rivera
authors.forename: Oscar
authors.initials: O
authors.lastname: Chávez-Talavera
authors.forename: Monserrat
authors.initials: M
authors.lastname: Sordo
authors.forename: Guillermina
authors.initials: G
authors.lastname: Avila
authors.forename: Ana
authors.initials: A
authors.lastname: Flisser
authors.forename: Patricia
authors.initials: P
authors.lastname: Ostrosky-Wegman
mesh_terms: D000284:Administration, Oral; D000818:Animals; D037282:Calreticulin / Q000276:immunology* / Q000627:therapeutic use*; D006224:Cricetinae; D004249:DNA Damage; D004195:Disease Models, Animal; D007114:Immunization; D015162:Micronucleus Tests; D011994:Recombinant Proteins / Q000235:genetics / Q000627:therapeutic use; D041201:Taenia solium / Q000502:physiology*; D013622:Taeniasis / Q000150:complications / Q000188:drug therapy*
chemical_list: D037282:Calreticulin; D011994:Recombinant Proteins