title: Prediction value of miR-483 and miR-214 in prognosis and multidrug resistance of esophageal squamous cell carcinoma.
abstract: OBJECTIVE
  Here, we have investigated the role of miR-483 and miR-214 in the prognosis and multidrug resistance (MDR) of esophageal squamous cell carcinoma.
  
  
  METHODS
  The expression of miR-483 and miR-214 was detected in 104 cases of esophageal cancer tissues and matched adjacent benign esophageal tissues by quantitative real-time PCR. The relation of microRNA expression with survival was statistically analyzed. The roles of miR-483 and miR-214 in MDR of esophageal squamous cell cancer cells were further evaluated.
  
  
  RESULTS
  The expression of miR-483 and miR-214 was found significantly upregulated in esophageal squamous cell cancer tissues. The expression levels of miR-483 and miR-214 showed an inverse correlation with overall survival. High expression of miR-483 and miR-214 might predict less chemotherapy effect. Downregulation of miR-483 and miR-214 could confer sensitivity of both P-glycoprotein-related and P-glycoprotein-nonrelated drugs to esophageal cancer cells, and it might induce increased accumulation of adriamycin (ADR) and decreased amount of ADR released.
  
  
  CONCLUSIONS
  miR-483 and miR-214 might play important roles in the pathogenesis of esophageal cancer and should be considered as potential targets for intervention in this malignancy.
authors.affiliation: Department of Colorectal Surgery, Tianjin Union Medicine Center, Tianjin, China. tianjinzhouyi@126.com
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhou
authors.forename: Liu
authors.initials: L
authors.lastname: Hong
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics / Q000378:metabolism; D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000188:drug therapy / Q000235:genetics*; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000627:therapeutic use; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D004938:Esophageal Neoplasms / Q000175:diagnosis / Q000188:drug therapy / Q000235:genetics*; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; C547397:MIRN214 microRNA, human; C548912:MIRN483 microRNA, human; D035683:MicroRNAs; D004317:Doxorubicin