title: Vesicle formation as a result of interaction between polymorphonuclear neutrophils and Staphylococcus aureus biofilm.
abstract: Staphylococcus aureus, a major opportunistic pathogen, is a leading cause of biofilm-related infections in clinical practice. Staphylococcal biofilms are highly resistant to antibacterial medicines and immune effector cells. The main result of our work is the discovery of nano-vesicles in the supernatant of the human neutrophil-S. aureus biofilm system. We also found that phospholipase C treatment causes complete destruction of these vesicles. While the addition of proteinase K led to a partial structural disorganization of the vesicles, DNase treatment did not influence the vesicle structure. These observations allowed us to conclude that phospholipids and proteins play a structure-forming role in the formation of these nano-vesicles. The vesicles demonstrated anti-biofilm activities when tested against Staphylococcus epidermidis (strains 178M and 328/5) biofilms, but were ineffective for S. aureus (strains 5983/2, 5663 and 18A) biofilms.
authors.affiliation: Department of Microbiology and Immunology, Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia.
authors.forename: Igor' V
authors.initials: IV
authors.lastname: Chebotar
authors.affiliation: Department of Microbiology and Immunology, Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia.
authors.forename: Evgenia D
authors.initials: ED
authors.lastname: Konchakova
authors.affiliation: Department of Microbiology and Immunology, Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia.
authors.forename: Andrey N
authors.initials: AN
authors.lastname: Maianskii
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000378:metabolism; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D002470:Cell Survival; D019286:Endopeptidase K / Q000378:metabolism; D005109:Extracellular Matrix; D054884:Host-Pathogen Interactions; D006801:Humans; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D009504:Neutrophils / Q000276:immunology / Q000382:microbiology* / Q000648:ultrastructure; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development / Q000502:physiology*; D013212:Staphylococcus epidermidis / Q000187:drug effects / Q000254:growth & development; D010738:Type C Phospholipases / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D010738:Type C Phospholipases; D019286:Endopeptidase K