title: Concurrent expression of C4.4A and Tenascin-C in tumor cells relates to poor prognosis of esophageal squamous cell carcinoma.
abstract: C4.4A is a glycolipid-anchored membrane protein expressed in several human malignancies. We recently found that C4.4A expression was associated with poor prognosis of esophageal squamous carcinoma cells (ESCCs), but the underlying mechanism is unknown. To uncover this, we performed PCR array analysis using the HCT116 cell line, a positive control for C4.4A expression and we found that Tenascin-C (TNC) among the many adhesion molecules and extracellular matrix proteins was the best candidate for C4.4A molecule induction. Based on in vitro studies using the TE8 esophageal cancer cells, we examined by immunohistochemistry TNC expression in 111 ESCCs. We found that the TNC-positive group (24.3%) had significantly poorer prognosis than the TNC-negative group in 5-year overall survival. We also found there was a significant correlation between TNC and C4.4A in ESCC tissues (P=0.007). Finally, we found that only the double-positive group for C4.4A and TNC had a significantly worse prognosis (P=0.005). Our data suggest that TNC expression in ESCC may in part explain why C4.4A is associated with a poor prognosis of ESCC since TNC can promote invasion and metastasis.
authors.affiliation: Department of Gastroenterological Surgery, Graduated School of Medicine, Osaka University, Osaka 565-0871, Japan.
authors.forename: Masahisa
authors.initials: M
authors.lastname: Ohtsuka
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Yamamoto
authors.forename: Ryota
authors.initials: R
authors.lastname: Oshiro
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Takahashi
authors.forename: Toru
authors.initials: T
authors.lastname: Masuzawa
authors.forename: Mamoru
authors.initials: M
authors.lastname: Uemura
authors.forename: Naotsugu
authors.initials: N
authors.lastname: Haraguchi
authors.forename: Junichi
authors.initials: J
authors.lastname: Nishimura
authors.forename: Taishi
authors.initials: T
authors.lastname: Hata
authors.forename: Makoto
authors.initials: M
authors.lastname: Yamasaki
authors.forename: Ichiro
authors.initials: I
authors.lastname: Takemasa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Miyata
authors.forename: Tsunekazu
authors.initials: T
authors.lastname: Mizushima
authors.forename: Shuji
authors.initials: S
authors.lastname: Takiguchi
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Doki
authors.forename: Masaki
authors.initials: M
authors.lastname: Mori
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology*; D015815:Cell Adhesion Molecules / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D004938:Esophageal Neoplasms / Q000378:metabolism* / Q000473:pathology*; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D058851:GPI-Linked Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D045325:HCT116 Cells; D006801:Humans; D008297:Male; D008875:Middle Aged; D034622:RNA Interference; D034741:RNA, Small Interfering; D019063:Tenascin / Q000096:biosynthesis / Q000378:metabolism*; D016896:Treatment Outcome
chemical_list: D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules; D058851:GPI-Linked Proteins; C472589:LYPD3 protein, human; D034741:RNA, Small Interfering; D019063:Tenascin