title: Expression, purification, crystallization and preliminary X-ray diffraction analysis of a core fragment of FlgG, a bacterial flagellar rod protein.
abstract: FlgG is a bacterial flagellar rod protein and constructs the distal rod connecting to the hook. FlgG of Salmonella enterica serovar Typhimurium is a 260-amino-acid protein composed of a folded core region and N- and C-terminal regions that are unfolded in solution. A core fragment of FlgG (FlgG47-227) was expressed, purified and crystallized. Crystals of native and SeMet-labelled FlgG47-227 were obtained by the sitting-drop vapour-diffusion technique with PEG MME 2000 as precipitant. The native crystal belonged to the primitive orthorhombic space group P212121, with unit-cell parameters a = 47.78, b = 68.94, c = 110.57 Å. The SeMet crystal also belonged to space group P212121, with unit-cell parameters a = 47.53, b = 67.04, c = 110.27 Å.
authors.affiliation: Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan. yumiko@fbs.osaka-u.ac.jp
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Saijo-Hamano
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Matsunami
authors.forename: Keiichi
authors.initials: K
authors.lastname: Namba
authors.forename: Katsumi
authors.initials: K
authors.lastname: Imada
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D003460:Crystallization; D005407:Flagella / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D019779:Salmonella enterica; D014961:X-Ray Diffraction
chemical_list: D001426:Bacterial Proteins
keywords: FlgG; Salmonella enterica; bacterial flagellum