title: In vivo gastric cancer targeting and imaging using novel symmetric cyanine dye-conjugated GX1 peptide probes.
abstract: To facilitate the translation of cancer fluorescence imaging into clinical practice, the development of stable and highly specific and sensitive targeted fluorescence probes with low toxicity is desirable. GX1, a gastric tumor angiogenesis marker candidate, holds promise in the target-specific delivery of molecular imaging probes for early gastric cancer detection in vivo. In this study, we describe the design and synthesis of a series of novel penta-methine cyanine dyes using the symmetric synthesis method and further conjugated the dyes with GX1, allowing specific binding to the vasculature of gastric cancer. This efficient synthetic route can decrease the undesired byproducts, while increasing yield. Furthermore, in vivo fluorescence imaging revealed that this novel targeted probe accumulates selectively in the tumor site of SGC-7901 subcutaneous xenograft models. The combination of such novel vasculature-targeted molecular probes with fluorescence imaging technology may improve early detection, metastasis detection, and antitumor angiogenesis therapy for gastric cancer.
authors.affiliation: School of Life Sciences and Technology, Xidian University, Xi'an, Shaanxi 710071, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xin
authors.forename: Xianghan
authors.initials: X
authors.lastname: Zhang
authors.forename: Jimin
authors.initials: J
authors.lastname: Liang
authors.forename: Limin
authors.initials: L
authors.lastname: Xia
authors.forename: Jipeng
authors.initials: J
authors.lastname: Yin
authors.forename: Yongzhan
authors.initials: Y
authors.lastname: Nie
authors.forename: Kaichun
authors.initials: K
authors.lastname: Wu
authors.forename: Jie
authors.initials: J
authors.lastname: Tian
mesh_terms: D002232:Carbocyanines / Q000737:chemistry*; D002478:Cells, Cultured; D004396:Coloring Agents / Q000737:chemistry*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008856:Microscopy, Fluorescence; D015335:Molecular Probes; D010455:Peptides / Q000737:chemistry*; D013274:Stomach Neoplasms / Q000188:drug therapy*
chemical_list: D002232:Carbocyanines; D004396:Coloring Agents; D015335:Molecular Probes; D010455:Peptides