title: Characterization of different food-isolated Enterococcus strains by MALDI-TOF mass fingerprinting.
abstract: Enterococcus is a controversial genus due to its great variability; this genus includes pathogenic strains, spoilage strains, and apparently safe strains including some probiotic strains. Previous studies focused on the characterization of strains of Enterococcus spp. involved in nosocomial infections. However, little research has been conducted on Enterococcus strains in foodstuffs. In the present work, 36 strains of different species of Enterococcus have been characterized by means of MALDI-TOF MS, resulting in highly specific mass spectral fingerprints. Characteristic peak masses common to certain bacterial species of Enterococcus have been identified. Thus, a peak at m/z 4426 ± 1 was assigned as a genus-specific biomarker. In addition, phyloproteomic relationships based on the mass spectral data were compared to the results of a phylogenetic analysis based on the 16S rRNA gene sequence. A better grouping at the species level was observed in the phyloproteomic tree, especially for the Enterococcus faecium group. Presumably, the assortment of some strains or ecotypes could be related to their ecological niche specialization. The approach described in this study leads the way toward the rapid and specific identification of different strains and species of Enterococcus in food based on molecular protein markers, aiming at the early detection of pathogenic strains and strains implicated in food poisoning or food spoilage.
authors.affiliation: Department of Analytical Chemistry, Nutrition and Food Science, School of Veterinary Sciences, University of Santiago de Compostela, Lugo, Spain.
authors.forename: Marcos
authors.initials: M
authors.lastname: Quintela-Baluja
authors.forename: Karola
authors.initials: K
authors.lastname: Böhme
authors.forename: Inmaculada C
authors.initials: IC
authors.lastname: Fernández-No
authors.forename: Stefano
authors.initials: S
authors.lastname: Morandi
authors.forename: Mohammed E
authors.initials: ME
authors.lastname: Alnakip
authors.forename: Sonia
authors.initials: S
authors.lastname: Caamaño-Antelo
authors.forename: Jorge
authors.initials: J
authors.lastname: Barros-Velázquez
authors.forename: Pilar
authors.initials: P
authors.lastname: Calo-Mata
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis / Q000737:chemistry; D003611:Dairy Products / Q000382:microbiology; D016983:Enterococcus / Q000737:chemistry / Q000145:classification* / Q000302:isolation & purification; D005516:Food Microbiology / Q000379:methods; D008460:Meat / Q000382:microbiology; D010802:Phylogeny; D020543:Proteome / Q000032:analysis / Q000737:chemistry; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D020543:Proteome
keywords: Bacterial species identification; Enterococcus; Food quality and safety; MALDI-TOF MS; Phyloproteomics