title: Thiol-reactive molecule with dual-emission-enhancement property for specific prestaining of cysteine containing proteins in SDS-PAGE.
abstract: 1-[4-(Bromomethyl)phenyl]-1,2,2-triphenylethene (2) was synthesized and evaluated for specific fluorescent prestaining of proteins containing cysteine (Cys) in SDS-PAGE. The molecule showed classic aggregation-induced emission (AIE) property in protein labeling and its quantum efficiency was further enhanced upon reacting with Cys. The parameters of reaction such as labeling time and concentration of dye and reducing reagent-tris(2-carboxyethyl)phosphine (TCEP) were examined to obtain the optimal labeling condition. In addition to its specific labeling effect, molecule 2 also showed its advantage over traditional self-quenching dyes through labeling Cys containing BSA with different dye/Cys ratios.
authors.forename: Yong
authors.initials: Y
authors.lastname: Yu
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Sijie
authors.initials: S
authors.lastname: Chen
authors.forename: Yuning
authors.initials: Y
authors.lastname: Hong
authors.forename: Ka Ming
authors.initials: KM
authors.lastname: Ng
authors.forename: Kathy Qian
authors.initials: KQ
authors.lastname: Luo
authors.forename: Ben Zhong
authors.initials: BZ
authors.lastname: Tang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D003545:Cysteine / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry; D010720:Phosphines / Q000737:chemistry; D011506:Proteins / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D003545:Cysteine; D005456:Fluorescent Dyes; D010720:Phosphines; D011506:Proteins; D013438:Sulfhydryl Compounds; C080938:tris(2-carboxyethyl)phosphine