title: Two-photon ratiometric fluorescent mapping of intracellular transport pathways of pH-responsive block copolymer micellar nanocarriers.
abstract: Ultrasensitive ratiometric fluorescent pH probes based on dual dye-labeled pH-responsive diblock copolymer micellar scaffold are constructed. The pH-sensitive emitting nature of BTPE dyes and emission turn-on of CMA moieties triggered by pH-actuated micelle-to-unimer of diblock scaffold synergistically contribute to the observed ≈250-fold changes of BTPE/CMA emission intensity ratios in the whole pH range. Two-photon ratiometric fluorescent pH mapping of intracellular gradients subjected by pH-responsive micellar nanoparticles in their endocytic pathway has been thus achieved.
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Hefei National Laboratory for Physical Sciences at the Microscale, Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei 230026, P. R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.forename: Jinming
authors.initials: J
authors.lastname: Hu
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Jin
authors.forename: Fan
authors.initials: F
authors.lastname: Jin
authors.forename: Shiyong
authors.initials: S
authors.lastname: Liu
mesh_terms: D004337:Drug Carriers / Q000737:chemistry* / Q000493:pharmacokinetics; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000493:pharmacokinetics; D056945:Hep G2 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008099:Liver / Q000737:chemistry / Q000378:metabolism; D008823:Micelles; D018613:Microscopy, Confocal; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry* / Q000493:pharmacokinetics; D013050:Spectrometry, Fluorescence
chemical_list: D004337:Drug Carriers; D005456:Fluorescent Dyes; D008823:Micelles; D011108:Polymers
keywords: diblock copolymer micelles; endocytic pathway; intracellular imaging; pH gradients; ratiometric quantification