title: Genetic characterization of VP1 gene of seven Sacbrood virus isolated from three provinces in northern China during the years 2008-2012.
abstract: The genetic diversity of seven northern China isolated Sacbrood virus strains (SBV) has been analyzed, and hypervariable regions of the VP1 gene of 7 SBV were sequenced and characterized, in order to obtain epidemiological and immunological information, and to suggest typing criteria for SBV. Sequence analysis of hypervariable regions of the VP1 gene in the genome of these isolates revealed a sequence homology of 91.0-99.3% among all seven local SBV isolates from Apis cerana from China, with a similarity of 93.3-100.0% in deduced amino acid sequences. These local isolates shared 87.4-92.8% sequence homology with six SBV reference strains in GenBank (including two SBV reference strains from Apis cerana from China), which represents a 91.8-97.6% similarity in deduced amino acid sequences. Genetic analysis also showed that five SBV strains from Apis cerana from China had a 13-amino-acid deletion at amino acid positions 287-299, and two SBV strains infecting the Korean honeybee had a 17-amino-acid deletion at amino acid positions 284-300 in comparison with other SBV. Phylogenetic analysis revealed two major groups (AC genotype SBV infecting Apis cerana and AM genotype SBV infecting Apis mellifera). The AC genotype could be further divided into subgroups. Based on the results of phylogenetic analysis, a similarity scan of SBV nucleotide sequences was carried out by using Simplot software and results in similar results. Our results suggest possible typing criteria for SBV based on the phylogenetic tree and sequence homology, and also that the virus has host specificity and regional variations.
authors.affiliation: Department of Laboratory Animal Center, Liaoning Medical University, Jinzhou 121001, China. nnxx8@yahoo.com.cn
authors.forename: Ma
authors.initials: M
authors.lastname: Mingxiao
authors.forename: Yin
authors.initials: Y
authors.lastname: Yanna
authors.forename: Xu
authors.initials: X
authors.lastname: Xiaoli
authors.forename: Zhang
authors.initials: Z
authors.lastname: Lin
authors.forename: Li
authors.initials: L
authors.lastname: Yongfei
authors.forename: Luan
authors.initials: L
authors.lastname: Zhidong
mesh_terms: D000818:Animals; D001516:Bees / Q000821:virology*; D002681:China; D016000:Cluster Analysis; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010802:Phylogeny; D012328:RNA Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D015678:Viral Structural Proteins / Q000235:genetics*
chemical_list: D012367:RNA, Viral; D015678:Viral Structural Proteins
keywords: Molecular epidemiology; Sacbrood virus; Typing criteria; VP1 gene