title: Effects of recombinant human AMH on SCF expression in human granulosa cells.
abstract: The aim of the present study was to investigate the effects of recombinant human anti-mullerian hormone (rhAMH) on Stem Cell Factor (SCF) expression in human granulosa cells (GCs). GCs were obtained from infertile patients undergoing IVF-ET cycles and cultured with 20 ng/ml of rhAMH. The levels of SCF mRNA and protein were detected in both matched and experimental group by real-time PCR, immunofluorescence, and ELISA, respectively, on day 4 of culture. We found that human GCs expressed SCF mRNA and protein, and SCF expression in the experimental group was significantly lower than that in the matched group (p < 0.05). We further showed that rhAMH inhibited SCF expression at mRNA and protein levels.
authors.affiliation: The Center for Reproductive Medicine, First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan, China, hr7424@126.com.
authors.forename: Rong
authors.initials: R
authors.lastname: Hu
authors.forename: Yan
authors.initials: Y
authors.lastname: Lou
authors.forename: Fei-Miao
authors.initials: FM
authors.lastname: Wang
authors.forename: Hui-Ming
authors.initials: HM
authors.lastname: Ma
authors.forename: Xing
authors.initials: X
authors.lastname: Wu
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Zhang
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: Ying-Pu
authors.initials: YP
authors.lastname: Sun
mesh_terms: D000328:Adult; D054304:Anti-Mullerian Hormone / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D002478:Cells, Cultured; D005260:Female; D006107:Granulosa Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology; D019089:Stem Cell Factor / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D011994:Recombinant Proteins; D019089:Stem Cell Factor; D054304:Anti-Mullerian Hormone