title: In vivo metabolism study of bergenin in rats by HPLC-QTOF mass spectrometry.
abstract: Bergenin is the major component of Ardisia creanta sims and Rodgersia sambucifolia hemsl with many biological activities. Although bergenin has been used to treat human diseases in China for man years, there is no report regarding its metabolism. This is the first report to separate and identify the metabolites of bergenin in vivo. In the study, HPLC/Q-TOF-MS/MS was used to investigate the metabolites of bergenin in vivo by analyzing the rat body fluid and feces samples. Three metabolites of bergenin were finally identified by the TIC chromatograms, and the structures were also confirmed by their MS(2) spectra.
authors.affiliation: Institute of Clinical Pharmacology, Qilu Hospital of Shandong University, Jinan, Shandong, 250012, People's Republic of China; Institute of Pharmacology, Medicine College of Shandong University, Jinan, Shandong, 250012, People's Republic of China.
authors.forename: Haojing
authors.initials: H
authors.lastname: Song
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Liu
authors.forename: Guiyan
authors.initials: G
authors.lastname: Yuan
authors.forename: Chunmin
authors.initials: C
authors.lastname: Wei
authors.forename: Wenjing
authors.initials: W
authors.lastname: Zhao
authors.forename: Rong
authors.initials: R
authors.lastname: Li
authors.forename: Benjie
authors.initials: B
authors.lastname: Wang
authors.forename: Ruichen
authors.initials: R
authors.lastname: Guo
mesh_terms: D000818:Animals; D031644:Ardisia / Q000737:chemistry; D001578:Benzopyrans / Q000032:analysis* / Q000097:blood / Q000378:metabolism* / Q000652:urine; D001646:Bile / Q000737:chemistry / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis / Q000378:metabolism; D005243:Feces / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D001578:Benzopyrans; D004365:Drugs, Chinese Herbal; C006741:bergenin
keywords: HPLC/Q-TOF-MS/MS; bergenin; in vivo; metabolites