title: Chaperone-interacting TPR proteins in Caenorhabditis elegans.
abstract: The ATP-hydrolyzing molecular chaperones Hsc70/Hsp70 and Hsp90 bind a diverse set of tetratricopeptide repeat (TPR)-containing cofactors via their C-terminal peptide motifs IEEVD and MEEVD. These cochaperones contribute to substrate turnover and confer specific activities to the chaperones. Higher eukaryotic genomes encode a large number of TPR-domain-containing proteins. The human proteome contains more than 200 TPR proteins, and that of Caenorhabditis elegans, about 80. It is unknown how many of them interact with Hsc70 or Hsp90. We systematically screened the C. elegans proteome for TPR-domain-containing proteins that likely interact with Hsc70 and Hsp90 and ranked them due to their similarity with known chaperone-interacting TPRs. We find C. elegans to encode many TPR proteins, which are not present in yeast. All of these have homologs in fruit fly or humans. Highly ranking uncharacterized open reading frames C33H5.8, C34B2.5 and ZK370.8 may encode weakly conserved homologs of the human proteins RPAP3, TTC1 and TOM70. C34B2.5 and ZK370.8 bind both Hsc70 and Hsp90 with low micromolar affinities. Mutation of amino acids involved in EEVD binding disrupts the interaction. In vivo, ZK370.8 is localized to mitochondria in tissues with known chaperone requirements, while C34B2.5 colocalizes with Hsc70 in intestinal cells. The highest-ranking open reading frame with non-conserved EEVD-interacting residues, F52H3.5, did not show any binding to Hsc70 or Hsp90, suggesting that only about 15 of the TPR-domain-containing proteins in C. elegans interact with chaperones, while the many others may have evolved to bind other ligands.
authors.affiliation: Center for Integrated Protein Science Munich and Department of Chemistry, Technische Universität München, 85747 Garching, Germany.
authors.forename: Veronika
authors.initials: V
authors.lastname: Haslbeck
authors.forename: Julia M
authors.initials: JM
authors.lastname: Eckl
authors.forename: Christoph J O
authors.initials: CJ
authors.lastname: Kaiser
authors.forename: Katharina
authors.initials: K
authors.lastname: Papsdorf
authors.forename: Martin
authors.initials: M
authors.lastname: Hessling
authors.forename: Klaus
authors.initials: K
authors.lastname: Richter
mesh_terms: D000818:Animals; D001665:Binding Sites; D017173:Caenorhabditis elegans / Q000201:enzymology*; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism*; D050883:HSC70 Heat-Shock Proteins / Q000378:metabolism*; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism*; D018832:Molecular Chaperones / Q000378:metabolism*; D016297:Mutagenesis, Site-Directed; D050505:Mutant Proteins / Q000378:metabolism; D011485:Protein Binding; D025941:Protein Interaction Mapping
chemical_list: D029742:Caenorhabditis elegans Proteins; D050883:HSC70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D018832:Molecular Chaperones; D050505:Mutant Proteins
keywords: AUC; C-terminal domain; CTD; HMMER; Hsc70; Hsp90; ITC; NGM; ORF; TPR; analytical ultracentrifugation; cochaperone; double-stranded RNA; dsRNA; isothermal titration calorimetry; nematode; nematode growth medium; open reading frame; tetratricopeptide repeat