title: Responses of the antioxidant system in QGY-7701 cells to the cytotoxicity and apoptosis induced by 1-octyl-3-methylimidazolium chloride.
abstract: This study aims to evaluate the cytotoxicity and responses of the cellular antioxidant system of 1-octyl-3-methylimidazolium chloride ([C8 mim][Cl]) on human hepatocarcinoma QGY-7701 cells. The results show that [C8 mim][Cl] can inhibit QGY-7701 cell growth and decrease their viabilities in a dose-dependent manner. The results also reveal that [C8 mim][Cl] exposure can induce apoptosis in the [C8 mim][Cl]-treated QGY-7701 cells. In addition, the results of biochemical assays show that [C8 mim][Cl] exposure causes overproduction of reactive oxygen species (ROS), inhibits superoxide dismutase (SOD) and catalase (CAT) activities, decreases reduced glutathione (GSH) content, and increases the cellular malondialdehyde (MDA) level. These results suggest that ROS-mediated oxidative stress may be responsible for the apoptosis induced by [C8 mim][Cl] in QGY-7701 cells.
authors.affiliation: Research Center of Stem Cell and Biotherapy Technology, Xinxiang Medical University, Henan 453003, People's Republic of China.
authors.forename: Changqin
authors.initials: C
authors.lastname: Jing
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Li
authors.forename: Jihong
authors.initials: J
authors.lastname: Zhang
authors.forename: Jianji
authors.initials: J
authors.lastname: Wang
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects*; D001881:Borates / Q000633:toxicity*; D002374:Catalase / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D007093:Imidazoles / Q000633:toxicity*; D008315:Malondialdehyde / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D012997:Solvents / Q000633:toxicity; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: C528768:1-octyl-3-methylimidazolium; D000975:Antioxidants; D001881:Borates; D007093:Imidazoles; D017382:Reactive Oxygen Species; D012997:Solvents; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase; D005978:Glutathione