title: Modified enzyme-linked immunosorbent assay strategy using graphene oxide sheets and gold nanoparticles functionalized with different antibody types.
abstract: Gold nanoparticles (GNPs) and graphene oxide (GO) sheets are excellent nano carriers in many analytical methods. In this study, a modified enzyme-linked immunosorbent assay (ELISA) strategy was developed using antibody-functionalized GO sheets and GNPs. This modification significantly reduced the limit of detection (LOD) and cost greatly of this assay. The applicability of the method was demonstrated by detecting HSP70 in a human serum sample. This result suggests that the 3G-ELISA method is feasible to detect an antigen in a complex mixture, and the LOD is up to 64-fold and the cost is as low as one-tenth of the conventional ELISA method.
authors.affiliation: State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing 102206, P. R. China.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Lin
authors.forename: Yingfu
authors.initials: Y
authors.lastname: Liu
authors.forename: Jingrui
authors.initials: J
authors.lastname: Huo
authors.forename: Aihong
authors.initials: A
authors.lastname: Zhang
authors.forename: Yiting
authors.initials: Y
authors.lastname: Pan
authors.forename: Haihong
authors.initials: H
authors.lastname: Bai
authors.forename: Zhang
authors.initials: Z
authors.lastname: Jiao
authors.forename: Tian
authors.initials: T
authors.lastname: Fang
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Yun
authors.initials: Y
authors.lastname: Cai
authors.forename: Qingming
authors.initials: Q
authors.lastname: Wang
authors.forename: Yangjun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Qian
mesh_terms: D000906:Antibodies / Q000032:analysis / Q000097:blood*; D015374:Biosensing Techniques / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis / Q000097:blood; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*
chemical_list: D000906:Antibodies; D018840:HSP70 Heat-Shock Proteins; D010087:Oxides; D006046:Gold; D006108:Graphite