title: Hepatoprotective and antioxidant activity of pentagamavunon-0 against carbon tetrachloride-induced hepatic injury in rats.
abstract: OBJECTIVE
  To investigate the hepatoprotective and antioxidant activity of pentagamavunon-0(PGV-0) against CCl4-induced hepatic injury in rats.
  
  
  METHODS
  The groups of animals were administered with PGV-0 at the doses 2.5, 5, 10, and 20 mg/kg b.w., p.o. once in a day for 6 days and at day 7 the animals were administrated with carbon tetrachloride (CCl) (20%, 2 mL/kg b.w. in liquid paraffin (i.p.). The effect of PGV-0 on serum transaminase (SGPT), alkaline phosphates (ALP) and total bilirubin were determined in CCl4-induced hepatotoxicity in rats. Further, the effects of PGV-0 on glutathione (GSH) content, catalase (CAT) and NO free radical scavenging activity also were investigated.
  
  
  RESULTS
  The results demonstrated that PGV-0 significantly reduced the activity of SGPT, serum ALP and total bilirubin in CCl4 induced rat hepatotoxicity. PGV-0 has effect on the antioxidant and free radical defense system. It prevented the depletion level of GSH and decrease activity of CAT in CCl4-induced liver injury in rats. PGV-0 also demonstrated the free radical scavenger effects on NO free radical scavenging activity with ES value of 32.32 μM.
  
  
  CONCLUSIONS
  All of our findings suggests that PGV-0 could protect the liver cells from CCl4-induced liver damages and the mechanism may through the antioxidative effect of PGV-0 to prevent the accumulation of free radicals and protect the liver damage.
authors.affiliation: Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Gadjah Mada University, Sekip Utara Yogyakarta-55281, Indonesia. ariefnr@gadjahmada.edu
authors.forename: Arief
authors.initials: A
authors.lastname: Nurrochmad
authors.forename: Supardjan Amir
authors.initials: SA
authors.lastname: Margono
authors.lastname: Sardjiman
authors.forename: Arief Rahman
authors.initials: AR
authors.lastname: Hakim
authors.lastname: Ernawati
authors.forename: Erna
authors.initials: E
authors.lastname: Kurniawati
authors.forename: Erva
authors.initials: E
authors.lastname: Fatmawati
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002252:Carbon Tetrachloride Poisoning / Q000188:drug therapy* / Q000378:metabolism / Q000517:prevention & control; D002374:Catalase / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000378:metabolism / Q000517:prevention & control; D003474:Curcumin / Q000031:analogs & derivatives / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; C587071:pentagamavunon-0; D009569:Nitric Oxide; D002374:Catalase; D005978:Glutathione; D003474:Curcumin