title: Crystallization of recombinant green mamba ρ-Da1a toxin during a lyophilization procedure and its structure determination.
abstract: ρ-Da1a toxin from eastern green mamba (Dendroaspis angusticeps) venom is a polypeptide of 65 amino acids with a strong affinity for the G-protein-coupled α(1A)-adrenoceptor. This neurotoxin has been crystallized from resolubilized lyophilized powder, but the best crystals grew spontaneously during lyophilization. The crystals belonged to the trigonal space group P3(1)21, with unit-cell parameters a = b = 37.37, c = 66.05 Å, and diffracted to 1.95 Å resolution. The structure solved by molecular replacement showed strong similarities to green mamba muscarinic toxins.
authors.affiliation: CEA, DSV, iBiTec-S, Service d'Ingénierie Moléculaire des Protéines, 91191 Gif-sur-Yvette, France.
authors.forename: Arhamatoulaye
authors.initials: A
authors.lastname: Maïga
authors.forename: Laura
authors.initials: L
authors.lastname: Vera
authors.forename: Charles
authors.initials: C
authors.lastname: Marchetti
authors.forename: Alain
authors.initials: A
authors.lastname: Lorphelin
authors.forename: Laurent
authors.initials: L
authors.lastname: Bellanger
authors.forename: Gilles
authors.initials: G
authors.lastname: Mourier
authors.forename: Denis
authors.initials: D
authors.lastname: Servent
authors.forename: Nicolas
authors.initials: N
authors.lastname: Gilles
authors.forename: Enrico Adriano
authors.initials: EA
authors.lastname: Stura
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003460:Crystallization; D004546:Elapid Venoms / Q000737:chemistry* / Q000235:genetics*; D017815:Elapidae; D005612:Freeze Drying; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry* / Q000235:genetics*; D017510:Protein Folding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: C549220:AdTx1 peptide, Dendroaspis angusticep; D004546:Elapid Venoms; D010455:Peptides; D011994:Recombinant Proteins
keywords: lyophilization; three-finger fold toxin; weak data