title: Evaluating biomarkers of neuronal degeneration and neuroinflammation in CSF of patients with multiple sclerosis-osteopontin as a potential marker of clinical severity.
abstract: Biomarkers capable of predicting the clinical course and the rate of disease progression in multiple sclerosis are currently unavailable. Our objective was to examine if the levels of proteins associated with axonal and neuronal degeneration (Tau, p-Tau and β-amyloid(1-42)) and T-cell-mediated autoimmunity (osteopontin) are altered in the cerebrospinal fluid (CSF) of MS patients, and to assess their potential in reflecting the clinical severity and predicting the progression and clinical evolution of early MS. The CSF samples collected from patients presenting with different clinical forms of MS were evaluated by enzyme-linked immunosorbent assays. The patients were regularly followed-up and their clinical status was re-evaluated 5 years after sampling. The results demonstrated that while CSF levels of Tau, p-Tau and β-amyloid(1-42) did not differ between MS and Control groups, the levels of osteopontin were significantly elevated in MS patients. This increase was associated with the presence of a relapse and correlated with clinical severity, which findings were independent of age and blood-CSF barrier function. However, none of the examined protein levels differed significantly between groups with different clinical evolutions and no positive correlations with clinical progression could be detected. We conclude that Tau, p-Tau and β-amyloid(1-42) are inappropriate as biomarkers in MS. This is the first report on CSF osteopontin as an independent marker of clinical severity in definite MS.
authors.affiliation: Department of Neurology, University of Szeged, Semmelweis u. 6, H-6725 Szeged, Hungary.
authors.forename: Levente
authors.initials: L
authors.lastname: Szalardy
authors.forename: Denes
authors.initials: D
authors.lastname: Zadori
authors.forename: Mihaela
authors.initials: M
authors.lastname: Simu
authors.forename: Krisztina
authors.initials: K
authors.lastname: Bencsik
authors.forename: Laszlo
authors.initials: L
authors.lastname: Vecsei
authors.forename: Peter
authors.initials: P
authors.lastname: Klivenyi
mesh_terms: D000293:Adolescent; D000328:Adult; D016564:Amyloid beta-Protein Precursor / Q000134:cerebrospinal fluid*; D015415:Biomarkers; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000150:complications*; D009410:Nerve Degeneration / Q000134:cerebrospinal fluid* / Q000209:etiology*; D053495:Osteopontin / Q000134:cerebrospinal fluid*; D011237:Predictive Value of Tests; D012720:Severity of Illness Index; D013223:Statistics as Topic; D055815:Young Adult; D016875:tau Proteins / Q000134:cerebrospinal fluid*
chemical_list: D016564:Amyloid beta-Protein Precursor; D015415:Biomarkers; D016875:tau Proteins; D053495:Osteopontin
keywords: Beta-amyloid; Biomarkers; Cerebrospinal fluid; ELISA; Multiple sclerosis; Osteopontin; Tau