title: Transcriptomics approaches in the early Arabidopsis embryo.
abstract: Early plant embryogenesis condenses the fundamental processes underlying plant development into a short sequence of predictable steps. The main tissues, as well as stem cells for their post-embryonic maintenance, are specified through genetic control networks. A key question is how cell fates are instructed by unique cellular transcriptomes, and important insights have recently been gained through cell type-specific transcriptomics during post-embryonic development. However, the poor accessibility and small size of Arabidopsis (Arabidopsis thaliana) embryos have obstructed similar progress during embryogenesis. Here, we review the current situation in plant embryo transcriptomics, and discuss how the recent development of novel cell-specific analysis technologies will enable the identification of cellular transcriptomes in the early Arabidopsis embryo.
authors.affiliation: Laboratory of Biochemistry, Wageningen University, The Netherlands.
authors.forename: Joakim
authors.initials: J
authors.lastname: Palovaara
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Saiga
authors.forename: Dolf
authors.initials: D
authors.lastname: Weijers
mesh_terms: D017360:Arabidopsis / Q000196:embryology* / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics*; D002454:Cell Differentiation; D020869:Gene Expression Profiling / Q000379:methods*; D018506:Gene Expression Regulation, Plant; D009928:Organ Specificity; D012639:Seeds / Q000196:embryology / Q000235:genetics
chemical_list: D029681:Arabidopsis Proteins
keywords: Arabidopsis; cell fate specification; embryogenesis; transcriptomics