title: Enzyme-directed assembly of a nanoparticle probe in tumor tissue.
abstract: Enzyme-directed assembly in vivo: A targeting strategy is demonstrated, which leads to an active accumulation of nanoparticles by virtue of an assembly event specific to endogenous, enzymatic biochemical signals associated with tumor tissue. The viability of this approach is examined through a proof-of-concept study showing enzyme-directed particle targeting and accumulation in human xenograft tumors in mice following intravenous injection, and the retention of particles is demonstrated within tumors for extended periods of time.
authors.affiliation: Department of Chemistry & Biochemistry, University of California, San Diego, La Jolla, CA 92093, USA.
authors.forename: Miao-Ping
authors.initials: MP
authors.lastname: Chien
authors.forename: Matthew P
authors.initials: MP
authors.lastname: Thompson
authors.forename: Christopher V
authors.initials: CV
authors.lastname: Barback
authors.forename: Ti-Hsuan
authors.initials: TH
authors.lastname: Ku
authors.forename: David J
authors.initials: DJ
authors.lastname: Hall
authors.forename: Nathan C
authors.initials: NC
authors.lastname: Gianneschi
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D051379:Mice; D015335:Molecular Probes / Q000737:chemistry* / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D010455:Peptides / Q000737:chemistry; D011108:Polymers / Q000737:chemistry
chemical_list: D015335:Molecular Probes; D010455:Peptides; D011108:Polymers; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9
keywords: enzyme-responsive; in vivo probe; micelle; nanoparticles; peptide