title: Electrochemical impedimetric immunosensor for the detection of measles-specific IgG antibodies after measles infections.
abstract: The detection of measles-specific primary antibodies (IgG) using electrochemical impedimetric immunosensors is reported. The optimum conditions for electrode saturation were reached after 40 min for 1 μg ml(-1) antibody concentrations. Surface roughness using AFM increased with each immobilization or antigen-antibody reaction step clearly confirming the surface modification and recognition between antigen and antibody. The human serum (HS) and new-born calf serum (NCS) spiked with antigen-specific antibody were studied to mimic the real sample analysis. The HS and NCS sera containing antibodies due to measles exhibited correlation between the increasing antibody serum concentrations and the charge-transfer resistance (electrochemically measured). This work clearly showed the potential use of impedance as the preferred electrochemical method for detecting measles-antibodies in label-free manner.
authors.affiliation: Nanotechnology Innovation Centre, Advanced Materials Division, Mintek, Private Bag X3015, Randburg 2125, South Africa. philanim@mintek.co.za
authors.forename: Philani
authors.initials: P
authors.lastname: Mashazi
authors.forename: Phumlani
authors.initials: P
authors.lastname: Tetyana
authors.forename: Sibulelo
authors.initials: S
authors.lastname: Vilakazi
authors.forename: Tebello
authors.initials: T
authors.lastname: Nyokong
mesh_terms: D000818:Animals; D000936:Antigen-Antibody Complex; D000941:Antigens / Q000097:blood / Q000276:immunology; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D055664:Electrochemical Techniques / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000032:analysis* / Q000276:immunology; D008457:Measles / Q000097:blood* / Q000175:diagnosis / Q000276:immunology; D008459:Measles virus / Q000276:immunology*; D012680:Sensitivity and Specificity
chemical_list: D000936:Antigen-Antibody Complex; D000941:Antigens; D007074:Immunoglobulin G
keywords: Immunosensors; Impedance; Measles-antigen; Measles-specific antibodies