title: Localization and function of KLF4 in cytoplasm of vascular smooth muscle cell.
abstract: The Krüppel-like factor 4 is a DNA-binding transcriptional regulator that regulates a diverse array of cellular processes, including development, differentiation, proliferation, and apoptosis. The previous studies about KLF4 functions mainly focused on its role as a transcription factor, its functions in the cytoplasm are still unknown. In this study, we found that PDGF-BB could prompt the translocation of KLF4 to the cytoplasm through CRM1-mediated nuclear export pathway in vascular smooth muscle cells (VSMCs) and increased the interaction of KLF4 with actin in the cytoplasm. Further study showed that both KLF4 phosphorylation and SUMOylation induced by PDGF-BB participates in regulation of cytoskeletal organization by stabilizing the actin cytoskeleton in VSMCs. In conclusion, these results identify that KLF4 participates in the cytoskeletal organization by stabilizing cytoskeleton in the cytoplasm of VSMCs.
authors.affiliation: Department of Biochemistry and Molecular Biology, The Key Laboratory of Neurobiology and Vascular Biology, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Bin
authors.initials: B
authors.lastname: Zheng
authors.forename: Xin-Hua
authors.initials: XH
authors.lastname: Zhang
authors.forename: Chan-Juan
authors.initials: CJ
authors.lastname: Nie
authors.forename: Yong-Hui
authors.initials: YH
authors.lastname: Li
authors.forename: Jin-Kun
authors.initials: JK
authors.lastname: Wen
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects / Q000378:metabolism; D000199:Actins / Q000378:metabolism; D021581:Active Transport, Cell Nucleus / Q000187:drug effects; D000818:Animals; D000077214:Becaplermin; D015153:Blotting, Western; D002467:Cell Nucleus / Q000378:metabolism; D002478:Cells, Cultured; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D028884:Karyopherins / Q000378:metabolism; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism*; D008297:Male; D018613:Microscopy, Confocal; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism*; D032389:Myocytes, Smooth Muscle / Q000187:drug effects / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D011485:Protein Binding / Q000187:drug effects; D020574:Proto-Oncogene Proteins c-sis / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism; D058207:Sumoylation / Q000187:drug effects; D000097606:Exportin 1 Protein
chemical_list: D000199:Actins; D000077214:Becaplermin; D028884:Karyopherins; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors; D020574:Proto-Oncogene Proteins c-sis; D018160:Receptors, Cytoplasmic and Nuclear; D000097606:Exportin 1 Protein; C000715467:KLF4 protein, human; C000715469:Klf4 protein, rat