title: Protective and anti-pathology effects of Sm fructose-1,6-bisphosphate aldolase-based DNA vaccine against schistosoma mansoni by changing route of injection.
abstract: This study aimed to evaluate the efficacy of fructose-1,6-bis phosphate aldolase (SMALDO) DNA vaccination against Schistosoma mansoni infection using different routes of injection. The SMALDO has been cloned into the eukaryotic expression vector pcDNA3.1/V5-His TOPO-TA and was used in injecting Swiss albino mice intramuscularly (IM), subcutaneously (SC), or intraperitoneally (IP) (50 µg/mouse). Mice vaccinated with non-recombinant pcDNA3.1 served as controls. Each group was immunized 4 times at weeks 0, 2, 4, and 6. Two weeks after the last booster dose, all mice groups were infected with 80 S. mansoni cercariae via tail immersion. At week 8 post-infection, animals were sacrificed for assessment of parasitological and histopathological parameters. High anti-SMALDO IgG antibody titers were detected in sera of all vaccinated groups (P<0.01) compared to the control group. Both the IP and SC vaccination routes resulted in a significant reduction in worm burden (46.2% and 28.9%, respectively, P<0.01). This was accompanied by a significant reduction in hepatic and intestinal egg counts (41.7% and 40.2%, respectively, P<0.01) in the IP group only. The number of dead eggs was significantly increased in both IP and IM groups (P<0.01). IP vaccination recorded the highest significant reduction in granuloma number and diameter (54.7% and 29.2%, respectively, P<0.01) and significant increase in dead miracidia (P<0.01). In conclusion, changing the injection route of SMALDO DNA vaccination significantly influenced the efficacy of vaccination. SMALDO DNA vaccination via IP route could be a promising protective and anti-pathology vaccine candidate against S. mansoni infection.
authors.affiliation: Biochemistry Department, Theodor Bilharz Research Institute, Giza, Egypt.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Saber
authors.forename: Tarek
authors.initials: T
authors.lastname: Diab
authors.forename: Olft
authors.initials: O
authors.lastname: Hammam
authors.forename: Amr
authors.initials: A
authors.lastname: Karim
authors.forename: Amina
authors.initials: A
authors.lastname: Medhat
authors.forename: Mamdouh
authors.initials: M
authors.lastname: Khela
authors.forename: Ehab
authors.initials: E
authors.lastname: El-Dabaa
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D004195:Disease Models, Animal; D005260:Female; D005634:Fructose-Bisphosphate Aldolase / Q000235:genetics / Q000276:immunology*; D006651:Histocytochemistry; D007074:Immunoglobulin G / Q000097:blood; D007273:Injections, Intramuscular; D007274:Injections, Intraperitoneal; D007279:Injections, Subcutaneous; D051379:Mice; D059208:Parasite Load; D012550:Schistosoma mansoni / Q000201:enzymology / Q000235:genetics / Q000276:immunology*; D012555:Schistosomiasis mansoni / Q000276:immunology / Q000469:parasitology / Q000473:pathology / Q000517:prevention & control*; D014611:Vaccination / Q000379:methods; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; D007074:Immunoglobulin G; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic; D005634:Fructose-Bisphosphate Aldolase
keywords: DNA vaccine; Schistosoma mansoni; different vaccination route; fructose-1,6-bis phosphate aldolase