title: Evaluation of amotosalem treated platelets over 7 days of storage with an automated cytometry assay panel.
abstract: BACKGROUND
  Pathogen Inactivation allows to overcome microbial contamination and growth related to storage of platelets concentrates (PC) at room temperature. The aim of our study was to evaluate the platelet storage lesion extending the storage period of pathogen inactivated platelet concentrates over 7 days using an automated cytometry assay panel.
  
  
  METHODS
  We analyzed 43 concentrates subjected to pathogen inactivation (CPPI) at 3, 5 and 7 days evaluating: platelet count, mean platelet volume, platelets at low optical density, platelets at high density, GPIIb-IIIa glycoprotein, platelet microparticles, lactate dehydrogenase. The collection bags (Fenwal) and the IBS kit made in PL2410/PL2411 are approved for the conservation of PC up to 7 days. Data analysis was performed with anova test.
  
  
  RESULTS
  All the parameters except small platelets and PMP were statistically different among day 7 vs. 3 and day 7 vs. 5.
  
  
  CONCLUSIONS
  Our study showed a progressive modification of pathogen inactivated platelet concentrates observed up to 7 days. The persistence of the secretory pool and the presence of the platelet membrane fibrinogen receptor suggest the persistence of a potential hemostatic efficacy. Clinical studies are necessary to directly correlate this type of analysis to 24 h recovery or survival of transfused platelets in humans.
authors.affiliation: Department of Clinical Pathology A.R.N.A.S. Civico, Laboratory of Clinical Pathology, Palermo, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Diquattro
authors.forename: G
authors.initials: G
authors.lastname: De Francisci
authors.forename: R
authors.initials: R
authors.lastname: Bonaccorso
authors.forename: A M
authors.initials: AM
authors.lastname: Tagliavia
authors.forename: M
authors.initials: M
authors.lastname: Marcatti
authors.forename: B
authors.initials: B
authors.lastname: Palma
authors.forename: R
authors.initials: R
authors.lastname: Agliastro
mesh_terms: D001792:Blood Platelets / Q000187:drug effects* / Q000378:metabolism; D001793:Blood Preservation; D005434:Flow Cytometry; D011564:Furocoumarins / Q000494:pharmacology*; D006801:Humans; D016130:Immunophenotyping; D039661:Integrin beta3 / Q000378:metabolism; D010641:Phenotype; D040201:Platelet Membrane Glycoprotein IIb / Q000378:metabolism; D013997:Time Factors
chemical_list: D011564:Furocoumarins; D039661:Integrin beta3; D040201:Platelet Membrane Glycoprotein IIb; C118577:amotosalen
keywords: Platelets; flow cytometry; laboratory automation; platelet transfusion