title: Reverse genetics identifies the product of open reading frame 4 as an essential particle assembly factor of Nyamanini virus.
abstract: We established a reverse genetics system for Nyamanini virus (NYMV) and recovered green fluorescent protein (GFP)-expressing virus from full-length cDNA. Using this technology, we assessed the functions of two poorly characterized viral genes. NYMV lacking open reading frame 2 (ORF2) could not be rescued, whereas virus lacking ORF4 was replication competent. ORF4-deficient NYMV readily established a persisting noncytolytic infection but failed to produce infectious viral particles, supporting the view that ORF4 represents an essential factor for NYMV particle assembly.
authors.affiliation: Department of Virology, University of Freiburg, Freiburg, Germany.
authors.forename: Marieke
authors.initials: M
authors.lastname: Herrel
authors.forename: Lars
authors.initials: L
authors.lastname: Haag
authors.forename: Josefina
authors.initials: J
authors.lastname: Nilsson
authors.forename: Peter
authors.initials: P
authors.lastname: Staeheli
authors.forename: Urs
authors.initials: U
authors.lastname: Schneider
mesh_terms: D018076:DNA, Complementary / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000378:metabolism; D018096:Mononegavirales / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics*; D059386:Reverse Genetics / Q000379:methods*; D019065:Virus Assembly / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D049452:Green Fluorescent Proteins