title: Gold immunochromatographic strips for enhanced detection of avian influenza and Newcastle disease viruses.
abstract: A new technique that uses gold immunochromatographic strips enhances the detection sensitivity by inducing the clustering of additional gold nanoparticles (AuNPs) around the immunogold particles immobilized on nitrocellulose strips. The additional AuNPs provide an intense signal that can be detected by the naked eye. The AuNPs were synthesized and conjugated to monoclonal antibodies using self-assembly. Other antibodies were immobilized in a defined detection zone on the nitrocellulose membrane. The detection principle is based on a "sandwich" immunoreaction, where gold-labeled antibodies serve as signal vehicles. To improve the sensitivity of the strips, we use a mixture of 1% HAuCl4 and 10 mmol L(-1) NH2OH·HCl to "enlarge" the gold nanoparticles. The detecting limits of Avian influenza virus (AIV) and Newcastle disease virus (NDV) are significantly increased. Compared with commercial test strips, this method is 100-fold more sensitive. This method is easy to perform and can be carried out on-site in test laboratories.
authors.affiliation: Chinese Academy of Inspection and Quarantine, Beijing 100123, China. starlary@163.com
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Li
authors.forename: Mingqiang
authors.initials: M
authors.lastname: Zou
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xue
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.forename: Beibei
authors.initials: B
authors.lastname: Li
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Qi
authors.forename: Yi
authors.initials: Y
authors.lastname: Yang
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D002846:Chromatography, Affinity / Q000295:instrumentation* / Q000379:methods*; D003102:Colloids / Q000032:analysis; D003429:Cross Reactions; D006046:Gold; D009980:Influenza A virus / Q000276:immunology / Q000302:isolation & purification*; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D009522:Newcastle disease virus / Q000276:immunology / Q000302:isolation & purification*; D011934:Reagent Strips; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors; D014773:Virology / Q000379:methods*
chemical_list: D000911:Antibodies, Monoclonal; D003102:Colloids; D011934:Reagent Strips; D006046:Gold