title: Identification of T- and B-Cell Subsets That Expand in the Central and Peripheral Lymphoid Organs during the Establishment of Nut Allergy in an Adjuvant-Free Mouse Model.
abstract: Nut allergies are potentially fatal and rarely outgrown for reasons that are not well understood. Phenotype of T- and B-cell subsets that expand during the early stages of nut allergy is largely unknown. Here we studied this problem using a novel mouse model of nut allergy. Mice were rendered hazelnut allergic by a transdermal sensitization/oral elicitation protocol. Using flow cytometry, the T- and B-cell phenotype in the bone marrow (BM), spleen, and the mesenteric lymph node (MLN) of allergic and control mice was analyzed. Nut allergic mice exhibited an expansion of CD4+ CD62L- T cells in BM and spleen; a similar trend was noted in the MLN. There was expansion of CD80+ B cells in BM and spleen and MLN and CD62L- cells in BM and spleen. Interestingly, among CD80+ B cells, significant proportion was CD73- particularly in the MLN. These data demonstrate that during the early establishment of hazelnut allergy there is (i) expansion of CD4+CD62L- T-cell subsets in both the BM and the periphery, (ii) expansion of CD80+ and CD62L- B-cell subsets in BM and the periphery, and (iii) a significant downregulation of CD73 on a subset of B cells in MLN.
authors.affiliation: Food Allergy and Immunology Laboratory, Michigan State University, East Lansing, MI 48823, USA ; Department of Food Science and Human Nutrition, Michigan State University, East Lansing, MI 48823, USA.
authors.forename: Babu
authors.initials: B
authors.lastname: Gonipeta
authors.forename: David
authors.initials: D
authors.lastname: Duriancik
authors.forename: Eunjung
authors.initials: E
authors.lastname: Kim
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Gardner
authors.forename: Venu
authors.initials: V
authors.lastname: Gangur