title: Antioxidant and anti-inflammatory in vitro activities of phenolic compounds from tropical highland blackberry (Rubus adenotrichos).
abstract: This study evaluates the antioxidant and anti-inflammatory activities in a polyphenol extract from blackberries. The antioxidant activity measured via oxygen radical absorbance capacity (ORAC) was higher for the blackberry extract (4339 ± 144 μM TE/g) than for quercetin and ellagic acid. The blackberry phenolic compounds protected liposomes and liver homogenates against lipid peroxidation; in both models, the antioxidant activity (IC₅₀ = 7.0 ± 0.5 and 20.3 ± 4.2 μg/mL, respectively) was greater than that found with Trolox. The extract inhibited superoxide production by NADPH oxidase in THP-1 cells and nitrite production in J774A.1 cells stimulated with LPS+IFNγ, with nitrite production decreasing after 4 h of incubation with the extract, mainly through a strong scavenging activity. However, 24 h of treatment reduced the amount of nitrites (IC₅₀ = 45.6 ± 1.2 μg/mL) because of a down-regulation of iNOS protein expression, as demonstrated by Western blotting. The inhibitory activities found in blackberry phenols suggest a potential beneficial effect against oxidative stress and inflammatory processes.
authors.affiliation: Departamento de Bioquímica, Facultad de Medicina, Universidad de Costa Rica , Código Postal 11501-2060 San José, Costa Rica.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Azofeifa
authors.forename: Silvia
authors.initials: S
authors.lastname: Quesada
authors.forename: Frederic
authors.initials: F
authors.lastname: Boudard
authors.forename: Marion
authors.initials: M
authors.lastname: Morena
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Cristol
authors.forename: Ana M
authors.initials: AM
authors.lastname: Pérez
authors.forename: Fabrice
authors.initials: F
authors.lastname: Vaillant
authors.forename: Alain
authors.initials: A
authors.lastname: Michel
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D000975:Antioxidants / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D002460:Cell Line; D003364:Costa Rica; D019587:Dietary Supplements; D005638:Fruit / Q000737:chemistry* / Q000254:growth & development; D006801:Humans; D015227:Lipid Peroxidation; D008099:Liver / Q000378:metabolism; D051379:Mice; D009000:Monocytes / Q000378:metabolism; D010636:Phenols / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D051381:Rats; D017207:Rats, Sprague-Dawley; D027824:Rosaceae / Q000737:chemistry* / Q000254:growth & development; D014329:Tropical Climate
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts