title: Mutascope: sensitive detection of somatic mutations from deep amplicon sequencing.
abstract: CONCLUSIONS
  We present Mutascope, a sequencing analysis pipeline specifically developed for the identification of somatic variants present at low-allelic fraction from high-throughput sequencing of amplicons from matched tumor-normal specimen. Using datasets reproducing tumor genetic heterogeneity, we demonstrate that Mutascope has a higher sensitivity and generates fewer false-positive calls than tools designed for shotgun sequencing or diploid genomes.
  
  
  BACKGROUND
  Freely available on the web at http://sourceforge.net/projects/mutascope/.
  
  
  BACKGROUND
  oharismendy@ucsd.edu
  
  
  BACKGROUND
  Supplementary data are available at Bioinformatics online.
authors.affiliation: Bioinformatics Graduate Program, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, USA.
authors.forename: Shawn E
authors.initials: SE
authors.lastname: Yost
authors.forename: Hakan
authors.initials: H
authors.lastname: Alakus
authors.forename: Hiroko
authors.initials: H
authors.lastname: Matsui
authors.forename: Richard B
authors.initials: RB
authors.lastname: Schwab
authors.forename: Kristen
authors.initials: K
authors.lastname: Jepsen
authors.forename: Kelly A
authors.initials: KA
authors.lastname: Frazer
authors.forename: Olivier
authors.initials: O
authors.lastname: Harismendy
mesh_terms: D004252:DNA Mutational Analysis / Q000379:methods*; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D020407:Internet; D009154:Mutation; D009369:Neoplasms / Q000235:genetics; D012984:Software