title: Structure of PatF from Prochloron didemni.
abstract: Patellamides are macrocyclic peptides with potent biological effects and are a subset of the cyanobactins. Cyanobactins are natural products that are produced by a series of enzymatic transformations and a common modification is the addition of a prenyl group. Puzzlingly, the pathway for patellamides in Prochloron didemni contains a gene, patF, with homology to prenylases, but patellamides are not themselves prenylated. The structure of the protein PatF was cloned, expressed, purified and determined. Prenylase activity could not be demonstrated for the protein, and examination of the structure revealed changes in side-chain identity at the active site. It is suggested that these changes have inactivated the protein. Attempts to mutate these residues led to unfolded protein.
authors.affiliation: Biomedical Sciences Research Complex, University of St Andrews, North Haugh, St Andrews, Fife KY16 9ST, Scotland.
authors.forename: Andrew F
authors.initials: AF
authors.lastname: Bent
authors.forename: Jesko
authors.initials: J
authors.lastname: Koehnke
authors.forename: Wael E
authors.initials: WE
authors.lastname: Houssen
authors.forename: Margaret C M
authors.initials: MC
authors.lastname: Smith
authors.forename: Marcel
authors.initials: M
authors.lastname: Jaspars
authors.forename: James H
authors.initials: JH
authors.lastname: Naismith
mesh_terms: D000595:Amino Acid Sequence; D003460:Crystallization; D008969:Molecular Sequence Data; D010456:Peptides, Cyclic / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D019691:Prochloron; D011485:Protein Binding / Q000502:physiology; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary
chemical_list: D010456:Peptides, Cyclic; C094789:patellamide F
keywords: cyanobactins; natural products; patellamide; prenyltransferases