title: Evaluation of stationary phases based on silica hydride for the analysis of drugs of abuse.
abstract: Several stationary phases based on silica hydride were evaluated as possible separation media for drugs of abuse. In the RP mode both a C18 and a phenyl column were tested using ACN/water and methanol/water mobile phases with formic acid or ammonium formate as modifiers. Detection was performed using MS, so separation of isobaric species was a factor in selecting the column, mobile phase composition, and gradient. A number of sample preparation procedures were also included as part of determining the most appropriate experimental protocol on these types of stationary phases. Morphine was used as a model for developing a protocol that would be suitable for the analysis of hydrophilic drugs.
authors.affiliation: Department of Chemistry, San Jose State University, San Jose, CA 95112, USA. joseph.pesek@sjsu.edu
authors.forename: Joseph J
authors.initials: JJ
authors.lastname: Pesek
authors.forename: Maria T
authors.initials: MT
authors.lastname: Matyska
authors.forename: Amy M
authors.initials: AM
authors.lastname: Kim
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D006801:Humans; D013287:Illicit Drugs / Q000652:urine*; D013058:Mass Spectrometry; D017640:Silicates / Q000737:chemistry*
chemical_list: D013287:Illicit Drugs; D017640:Silicates; C487012:silica hydride
keywords: Drug analysis; Hydride stationary phases; LC-MS