title: Effect of trans-ε-viniferin on in vitro porcine oocyte maturation and subsequent developmental competence in preimplantation embryos.
abstract: Trans-ε-viniferin is a naturally occurring polyphenol belonging to the stilbenoid family that has been isolated from Vitis amurensis, one of the most common wild grapes in Asia. We investigated the effects of trans-ε-viniferin on in vitro maturation (IVM) and developmental competence after in vitro fertilization (IVF) or parthenogenesis (PA). We observed that trans-ε-viniferin treatment during IVM did not improve nuclear maturation rates of oocytes in any group, but significantly increased (P<0.05) intracellular glutathione (GSH) levels and reduced reactive oxygen species (ROS) levels in the 0.5 μM treatment group. Trans-ε-viniferin treatment during IVM of recipient oocytes promoted higher (P<0.05) expression of DNA methyltransferase-1 (DNMT1) mRNA in the 0.5 μM treatment group as compared with the control group. However, the expression of essential transcriptional and apoptosis-related genes did not significantly differ from that of the control. In cumulus cells, pro-apoptosis gene expressions were changed as apoptosis decreased. Oocytes treated with trans-ε-viniferin during IVM did not have significantly different cleavage rates or blastocyst formation rates after PA, but total cell numbers were significantly higher (P<0.05) in the 0.5 and 5.0 μM treatment groups compared with those in the control group. IVF embryos showed similar results. In conclusion, these results indicate that trans-ε-viniferin treatment during porcine IVM increased the total cell number of blastocysts, possibly by increasing intracellular GSH synthesis, reducing ROS levels, increasing DNMT1 gene expression of oocytes and decreasing pro-apoptosis gene expressions of cumulus cells.
authors.affiliation: Laboratory of Veterinary Embryology and Biotechnology (VETEMBIO), College of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Republic of Korea.
authors.forename: Yubyeol
authors.initials: Y
authors.lastname: Jeon
authors.forename: Seong-Sung
authors.initials: SS
authors.lastname: Kwak
authors.forename: Seung-A
authors.initials: SA
authors.lastname: Cheong
authors.forename: Yeon Hee
authors.initials: YH
authors.lastname: Seong
authors.forename: Sang-Hwan
authors.initials: SH
authors.lastname: Hyun
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001572:Benzofurans / Q000494:pharmacology*; D001755:Blastocyst / Q000502:physiology*; D047108:Embryonic Development / Q000502:physiology; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary; D005978:Glutathione / Q000032:analysis / Q000378:metabolism; D059471:In Vitro Oocyte Maturation Techniques / Q000379:methods / Q000662:veterinary*; D008780:Methyltransferases / Q000032:analysis / Q000378:metabolism; D009865:Oocytes / Q000187:drug effects / Q000201:enzymology / Q000502:physiology*; D010312:Parthenogenesis / Q000502:physiology*; D011897:Random Allocation; D017382:Reactive Oxygen Species / Q000032:analysis / Q000378:metabolism; D013267:Stilbenes / Q000494:pharmacology*; D013552:Swine / Q000502:physiology*
chemical_list: D001572:Benzofurans; D017382:Reactive Oxygen Species; D013267:Stilbenes; C091891:epsilon-viniferin; D008780:Methyltransferases; D005978:Glutathione