title: [Normative research on bacteriostasis and relieving itching external therapeutic function of kochiae fructus].
abstract: OBJECTIVE
  To normalize bacteriostasis and relieving itching external therapeutic function of Kochiae Fructus.
  
  
  METHODS
  Itching guinea pig model caused by histamine, itching mice model, eczema guinea pig model caused by OVA, and inhibitory effect on pathogens in vitro were used to observe the itching threshold, symptoms and other related physiological index, as well as the inhibitory effect on the normal skin fungi by water extraction of Kochiae Fructus to evaluate the external therapeutic function of Kochiae Fructus.
  
  
  RESULTS
  The itching threshold of guinea pig itching model treated by water extraction of Kochiae Fructus at high, medium and low three dosage level, could be significantly increased when compared with negative control group (P < 0.05 or P < 0.01); Red speckle of OVA guinea pig model treated by water extraction of Kochiae Fructus at high, medium and low three dosage level, could be significantly decreased when compared with negative control group (P < 0.05 or P < 0.01); The number of itching and total time of itching within 30 minutes of mice model caused by R-glycose anhydride treated by water extraction of Kochiae Fructus at high, medium and low three dosage level, could be significantly decreased when compared with negative control group (P < 0.05 or P < 0.01); Several common skin fungi could be significantly inhibited by the water extraction of Kochiae Fructus. MIC of the water extraction of Kochiae Fructus on Trichophyton mentagrophytes, Trichophyton rubrum, Microsporum canis, Trichophyton violaceum, and Trichophyton schoenleini were 3.12%, 0.78%, 0.78%, 0.78%, 0.78%, respectively.
  
  
  CONCLUSIONS
  Kochiae fructus has the effect of bacteriostasis and relieving itching.
authors.affiliation: Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China.
authors.forename: Jian-Nong
authors.initials: JN
authors.lastname: Wang
authors.forename: Qing-Yun
authors.initials: QY
authors.lastname: Liu
authors.forename: Chun-Hai
authors.initials: CH
authors.lastname: Yao
authors.forename: Shao-Jun
authors.initials: SJ
authors.lastname: Chen
authors.forename: Li-Yan
authors.initials: LY
authors.lastname: Song
authors.forename: Guan
authors.initials: G
authors.lastname: Pin
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Shao
authors.forename: Yuan-Yao
authors.initials: YY
authors.lastname: She
authors.forename: Hui-Yan
authors.initials: HY
authors.lastname: Chi
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology*; D000982:Antipruritics / Q000008:administration & dosage / Q000494:pharmacology*; D003883:Arthrodermataceae / Q000187:drug effects*; D027467:Bassia scoparia / Q000737:chemistry*; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D004485:Eczema / Q000139:chemically induced / Q000188:drug therapy / Q000473:pathology; D005260:Female; D005638:Fruit / Q000737:chemistry; D006168:Guinea Pigs; D006632:Histamine / Q000008:administration & dosage; D008297:Male; D051379:Mice; D008826:Microbial Sensitivity Tests; D008864:Microsporum / Q000187:drug effects; D010047:Ovalbumin / Q000008:administration & dosage; D011537:Pruritus / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D012867:Skin / Q000187:drug effects / Q000473:pathology; D014249:Trichophyton / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D000982:Antipruritics; D004365:Drugs, Chinese Herbal; D006632:Histamine; D010047:Ovalbumin