title: Biodegradation of wool waste and keratinase production in scale-up fermenter with different strategies by Stenotrophomonas maltophilia BBE11-1.
abstract: A keratin-degrading strain Stenotrophomonas maltophilia BBE11-1 was grown in a 3-L batch fermenter containing wool waste as the main medium and cell growth rate was determined as the key factor to affect keratinase yield. Three strategies of temperature-shift procedure, two-stage DO control and fed-batch process were used to change growth rate. And a 62.2% improvement of keratinase yield was achieved. With the glucose fed-batch procedure in 30-L fermenter, keratinase production was significantly improved up to 117.7% (1728 U/ml) as compared with initial data (793.8 U/ml) in a 3-L fermenter and with much shortened fermentation time within 18 h. Significant structure changes and high levels of free amino acids from wool decomposition indicated the possible applications for wool waste management and fertilizer industry. The remarkable digestion of wool cuticle also suggested its potential utilization in textile industry.
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China. zhenfang89@hotmail.com
authors.forename: Zhen
authors.initials: Z
authors.lastname: Fang
authors.forename: Juan
authors.initials: J
authors.lastname: Zhang
authors.forename: Baihong
authors.initials: B
authors.lastname: Liu
authors.forename: Guocheng
authors.initials: G
authors.lastname: Du
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D061249:Batch Cell Culture Techniques; D001673:Biodegradation, Environmental / Q000187:drug effects; D019149:Bioreactors / Q000382:microbiology*; D001709:Biotechnology / Q000295:instrumentation* / Q000379:methods*; D005285:Fermentation / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D017711:Nonlinear Dynamics; D010447:Peptide Hydrolases / Q000096:biosynthesis*; D020615:Stenotrophomonas maltophilia / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D013997:Time Factors; D014866:Waste Products; D014935:Wool / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D014866:Waste Products; D010447:Peptide Hydrolases; C035855:keratinase; D005947:Glucose