title: Detecting Aelurostrongylus abstrusus-specific IgG antibody using an immunofluorescence assay.
abstract: Diagnosis of feline lungworm, Aelurostrongylus abstrusus, is typically achieved by identifying larvae in feces following concentration through flotation or using the Baermann technique. This work presents observations on the usefulness of an indirect immunofluorescence antibody assay for detection of antibodies to this parasite in the sera of infected cats. Using first-stage larvae of A abstrusus and sera from both experimentally and naturally infected cats, it was determined that the test was fairly sensitive and did not cross-react with serum from an Ancylostoma braziliense (hookworm)-infected cat.
authors.affiliation: Department of Immunology and Microbiology, Cornell University College of Veterinary Medicine, Ithaca, NY, USA.
authors.forename: Kaitlyn R
authors.initials: KR
authors.lastname: Briggs
authors.forename: Joseph P
authors.initials: JP
authors.lastname: Yaros
authors.forename: Janice L
authors.initials: JL
authors.lastname: Liotta
authors.forename: Araceli
authors.initials: A
authors.lastname: Lucio-Forster
authors.forename: Alice C Y
authors.initials: AC
authors.lastname: Lee
authors.forename: Dwight D
authors.initials: DD
authors.lastname: Bowman
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood*; D002371:Cat Diseases / Q000097:blood / Q000175:diagnosis / Q000469:parasitology*; D002415:Cats; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods / Q000662:veterinary*; D007074:Immunoglobulin G / Q000097:blood*; D008297:Male; D012680:Sensitivity and Specificity; D017183:Strongylida / Q000302:isolation & purification; D017206:Strongylida Infections / Q000175:diagnosis / Q000662:veterinary
chemical_list: D000909:Antibodies, Helminth; D007074:Immunoglobulin G