title: Dexamethasone-FITC staining application for measurement of circadian rhythmicity of glucocorticoid receptor expression in mouse living thymocyte subsets.
abstract: Glucocorticoids are involved in the regulation of immune homeostasis and thymopoiesis and the integration of the thymus function with the neuroendocrine system. Their regulatory function is closely related to glucocorticoid receptor (GCR) expression. The aim of this study was to develop a method for the measurement of GCR expression in mouse living thymocytes by flow cytometry. Using dexamethasone binding we have shown differences in GCR expression among thymocyte subsets and their dependence on the circadian rhythm.
authors.affiliation: Department of Immunology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Kowalik
authors.forename: Ewelina
authors.initials: E
authors.lastname: Kiernozek
authors.forename: Mariusz
authors.initials: M
authors.lastname: Kulinczak
authors.forename: Klaudia
authors.initials: K
authors.lastname: Brodaczewska
authors.forename: Ewa
authors.initials: E
authors.lastname: Kozlowska
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Gieczewska
authors.forename: Carlo
authors.initials: C
authors.lastname: Riccardi
authors.forename: Nadzieja
authors.initials: N
authors.lastname: Drela
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D002940:Circadian Rhythm / Q000276:immunology*; D003907:Dexamethasone / Q000032:analysis; D016650:Fluorescein-5-isothiocyanate / Q000032:analysis; D005456:Fluorescent Dyes / Q000032:analysis; D005786:Gene Expression Regulation / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D011965:Receptors, Glucocorticoid / Q000096:biosynthesis* / Q000235:genetics; D016176:T-Lymphocyte Subsets / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D060168:Thymocytes / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*
chemical_list: D005456:Fluorescent Dyes; D011965:Receptors, Glucocorticoid; D003907:Dexamethasone; D016650:Fluorescein-5-isothiocyanate
keywords: Circadian rhythm; Dexamethasone; Glucocorticoid receptor; Thymocyte subsets; Thymocytes