title: [Optimization of extraction process of Chrysanthemi Flos by multi-index orthogonal experiment].
abstract: OBJECTIVE
  To explore quantitative assessment indicators of Chrysanthemi Flos, and optimize the extraction process of Chrysanthemi Flos through orthogonal experimental design.
  
  
  METHODS
  The concentration of ethanol, amount of ethanol, extraction time and extraction frequency were selected as factors in the L9 (3(4)) orthogonal experiment. A comprehensive assessment was conducted with the peak area of the eight major common peaks in the fingerprint of Chrysanthemi Flos as the indicators.
  
  
  RESULTS
  The optimum extraction process was selected as follows: using ultrasonic extraction method, adding 30-fold ethanol with 80% concentration, extracting for 2 times for extraction, 40 min for each time.
  
  
  CONCLUSIONS
  The optimized extraction process is reliable, with controllable assessment indicators, which is significant to the standardization of the extraction process and quality control of Chrysanthemi Flos preparations.
authors.affiliation: Engineering Research Center for Processing Standardization of Traditional Chinese Medicine under Ministry of Education, Nanjing University of Chinese Medicine, Nanjing 210029, China.
authors.forename: Xing-Hai
authors.initials: XH
authors.lastname: Zhang
authors.forename: Huan
authors.initials: H
authors.lastname: Li
authors.forename: Kun-Ming
authors.initials: KM
authors.lastname: Qin
authors.forename: Xiao
authors.initials: X
authors.lastname: Liu
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Bao-Chang
authors.initials: BC
authors.lastname: Cai
mesh_terms: D005591:Chemical Fractionation / Q000379:methods*; D031202:Chrysanthemum / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D000431:Ethanol / Q000737:chemistry; D006358:Hot Temperature; D011786:Quality Control; D015203:Reproducibility of Results; D012995:Solubility; D013997:Time Factors
chemical_list: D004365:Drugs, Chinese Herbal; D000431:Ethanol