title: Development of poliovirus extraction method from stool extracts by using magnetic nanoparticles sensitized with soluble poliovirus receptor.
abstract: A method for extracting poliovirus (PV) from stool extracts was developed. Magnetic nanoparticles sensitized with soluble PV receptor efficiently extracted PV pseudovirus (>99% extraction) or endogenous infectious PVs (>90% extraction) from stool extracts. This method would be useful for extraction of PV from crude biological samples.
authors.affiliation: Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan. minetaro@nih.go.jp
authors.forename: Minetaro
authors.initials: M
authors.lastname: Arita
mesh_terms: D005243:Feces / Q000382:microbiology*; D006801:Humans; D008280:Magnetics; D053758:Nanoparticles; D017955:Poliovirus / Q000302:isolation & purification*; D011991:Receptors, Virus / Q000378:metabolism*; D014773:Virology / Q000379:methods*
chemical_list: D011991:Receptors, Virus; C066100:poliovirus receptor