title: Chicken cytochrome P450 1A5 is the key enzyme for metabolizing T-2 toxin to 3'OH-T-2.
abstract: The transmission of T-2 toxin and its metabolites into the edible tissues of poultry has potential effects on human health. We report that T-2 toxin significantly induces CYP1A4 and CYP1A5 expression in chicken embryonic hepatocyte cells. The enzyme activity assays of CYP1A4 and CYP1A5 heterologously expressed in HeLa cells indicate that only CYP1A5 metabolizes T-2 to 3'OH-T-2 by the 3'-hydroxylation of isovaleryl groups. In vitro enzyme assays of recombinant CYP1A5 expressed in DH5α further confirm that CYP1A5 can convert T-2 into TC-1 (3'OH-T-2). Therefore, CYP1A5 is critical for the metabolism of trichothecene mycotoxin in chickens.
authors.affiliation: Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou 510642, Guangdong, China. yqdeng@scau.edu.cn.
authors.forename: Shufeng
authors.initials: S
authors.lastname: Shang
authors.forename: Jun
authors.initials: J
authors.lastname: Jiang
authors.forename: Yiqun
authors.initials: Y
authors.lastname: Deng
mesh_terms: D000818:Animals; D001189:Aryl Hydrocarbon Hydroxylases / Q000235:genetics / Q000378:metabolism*; D055162:Biocatalysis; D002645:Chickens / Q000378:metabolism*; D002853:Chromatography, Liquid; D006367:HeLa Cells; D006801:Humans; D008658:Inactivation, Metabolic; D017433:Protein Structure, Secondary; D060888:Real-Time Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D021241:Spectrometry, Mass, Electrospray Ionization; D013605:T-2 Toxin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; C049149:3'-hydroxy-T-2 toxin; D001189:Aryl Hydrocarbon Hydroxylases; C108015:cytochrome P-450 CYP1A5; D013605:T-2 Toxin