title: So close and yet so far: mitochondria and peroxisomes are one but with specific talents.
abstract: Cellular compartmentalization of central metabolic pathways as lipid metabolism to mitochondria and peroxisomes enables high efficient control processes. The basis to understand mitochondrial or peroxisomal function is exactly to determine proteins physically present. For proteomic investigations of mouse liver organelles, we developed 2-DE reference maps covering the range pH 4-9, available under ( www.diabesityprot.org ). MALDI-TOF-MS/MS analyses identified a total of 799 (mitochondria) and 681 (peroxisome) protein spots resembling 323 and 293 unique proteins, respectively. Direct comparison of mitochondrial and peroxisomal proteins indicated an approximate overlap of 2/3 of identified proteins. Gene Ontologies (GO) of the identified proteins in respect to physical presence confirmed functional specifications within the organelles. The 2-DE organelle reference maps will aid to point out functional differences and similarities. Our observations suggest that for functional analyses metabolic alterations focusing on one organelle are not sufficient and parallel comparison of both organelles is to be preferred.
authors.affiliation: Institute of Clinical Biochemistry and Pathobiochemistry, German Diabetes Center at the Heinrich-Heine-University Deusseldorf, Leibniz Center for Diabetes Research, 40225 Düsseldorf, Germany.
authors.forename: Sonja
authors.initials: S
authors.lastname: Hartwig
authors.forename: Birgit
authors.initials: B
authors.lastname: Knebel
authors.forename: Simon
authors.initials: S
authors.lastname: Goeddeke
authors.forename: Cornelia
authors.initials: C
authors.lastname: Koellmer
authors.forename: Sylvia
authors.initials: S
authors.lastname: Jacob
authors.forename: Ulrike
authors.initials: U
authors.lastname: Nitzgen
authors.forename: Waltraud
authors.initials: W
authors.lastname: Passlack
authors.forename: Martina
authors.initials: M
authors.lastname: Schiller
authors.forename: Hans-Dieter
authors.initials: HD
authors.lastname: Dicken
authors.forename: Jutta
authors.initials: J
authors.lastname: Haas
authors.forename: Dirk
authors.initials: D
authors.lastname: Muller-Wieland
authors.forename: Stefan
authors.initials: S
authors.lastname: Lehr
authors.forename: Jorg
authors.initials: J
authors.lastname: Kotzka
mesh_terms: D000818:Animals; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D006863:Hydrogen-Ion Concentration; D007091:Image Processing, Computer-Assisted; D007525:Isoelectric Focusing; D008099:Liver / Q000737:chemistry*; D051379:Mice; D008810:Mice, Inbred C57BL; D008928:Mitochondria / Q000737:chemistry*; D058977:Molecular Sequence Annotation; D020675:Peroxisomes / Q000737:chemistry*; D020543:Proteome / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D020543:Proteome