title: Determination of whole transcription profiles and specific pathways in invasive ductal breast carcinoma.
abstract: Breast cancer is the most common cancer affecting women worldwide including Thailand. Whole transcription profiles of invasive ductal breast carcinoma (IDC) obtained by oligonucleotide microarray should lead to a better understanding of the molecular basis of IDCs, allow for examination of specific markers for diagnosis, and provide novel targets for therapy. This study aimed to detect the whole transcript expression of approximately 35,000 target genes in Thai breast cancer patients, using Affymetrix GeneChip(®) Exon 1.0 Sense Target Arrays. Analysis revealed that the differential expression profiles of 928 genes (423 up-regulated and 505 down-regulated genes) were 2-fold or greater (unpaired t-test, p < 0.05) in invasive ductal breast cancer, compared with normal tissues. The Gene Ontology (GO) databases support important associations in 17 gene sets with p-value < 1E-10 and ≥ 4-fold changes, involving the tumorigenic pathways of cell cycles, extracellular regions, as well as cellular component organization. Likewise, the TGFBR and IL-6 pathways contain gene expression with statistically significant changes in IDC.
authors.affiliation: Department of Tropical Nutrition & Food Science, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand.
authors.forename: Pasra
authors.initials: P
authors.lastname: Arnutti
authors.forename: Manas
authors.initials: M
authors.lastname: Kotepui
authors.forename: Wichitra
authors.initials: W
authors.lastname: Asanprakit
authors.forename: Phaibul
authors.initials: P
authors.lastname: Punyarit
authors.forename: Porntip
authors.initials: P
authors.lastname: Chavalitshewinkoon-Petmitr
authors.forename: Talabporn
authors.initials: T
authors.lastname: Harnroongroj
authors.forename: Songsak
authors.initials: S
authors.lastname: Petmitr
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics*; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D018270:Carcinoma, Ductal, Breast / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D053263:Gene Regulatory Networks; D020022:Genetic Predisposition to Disease; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D011237:Predictive Value of Tests; D011379:Prognosis; D015203:Reproducibility of Results
chemical_list: D014408:Biomarkers, Tumor
keywords: IL-6 pathway; Invasive ductal breast carcinoma; TGFBR; gene expression profile; oligonucleotide microarray