title: Interactions between exogenous FGF-2 and sulfonic groups: in situ characterization and impact on the morphology of human adipose-derived stem cells.
abstract: FGF-2 is often used as a supplement to stem cells culture medium aiming at preserving their self-renewal capacity and plasticity through the passages. However, little is known on the influence of the underlying substrate in these interactions. In this study, we have used mixed self-assembled monolayers with different ratios of -SO3H and -OH tail groups to investigate the influence of substrate properties (e.g., charge) on the FGF-2 adsorption and activity. QCM-D data demonstrated that, in the presence of -OH groups, the quantity of the adsorbed FGF-2 is proportional to the percentage of surface -SO3H groups. The bioactivity of the adsorbed FGF-2 follows the same tendency as demonstrated by its interactions with anti-FGF-2. Surprisingly, the adlayer of FGF-2 formed on the surface containing only SO3H-tailed SAMs was similar to the surface with 25% of -SO3H groups, demonstrating that FGF-2 adsorption is not solely driven by electrostatic interactions. We related these results with changes in the morphology of adipose-derived stem cells (ASCs) cultured on the same surfaces.
authors.affiliation: 3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Taipas, Guimarães, Portugal.
authors.forename: Sara
authors.initials: S
authors.lastname: Amorim
authors.forename: Ricardo A
authors.initials: RA
authors.lastname: Pires
authors.forename: Diana Soares
authors.initials: DS
authors.lastname: da Costa
authors.forename: Rui L
authors.initials: RL
authors.lastname: Reis
authors.forename: Iva
authors.initials: I
authors.lastname: Pashkuleva
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000328:Adult; D002478:Cells, Cultured; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects; D055815:Young Adult
chemical_list: D016222:Fibroblast Growth Factor 2