title: Epichromatin is conserved in Toxoplasma gondii and labels the exterior parasite chromatin throughout the cell cycle.
abstract: Toxoplasma gondii is an apicomplexan intracellular protozoan parasite responsible for toxoplasmosis, a disease with considerable medical and economic impact worldwide. Toxoplasma gondii cells never lose the nuclear envelope and their chromosomes do not condense. Here, we tested the murine monoclonal antibody PL2-6, which labels epichromatin (a conformational chromatin epitope based on histones H2A and H2B complexed with DNA), in T. gondii cultured in human fibroblasts. This epitope is present at the exterior chromatin surface of interphase nuclei and on the periphery of mitotic chromosomes in higher eukaryotes. PL2-6 reacted with T. gondii H2A and H2B histones in Western blot (WB) assays. In addition, the antibody reacted with the nuclear fraction of tachyzoites, as a single band coincident with H2B histone. In the T. gondii tachyzoite stage, PL2-6 also had peripheral nuclear localization, as observed by epifluorescence/confocal microscopy and immunoelectron microscopy. Confocal analysis showed that epichromatin is slightly polarized to one face of the parasite exterior chromatin surface. In replicating tachyzoites, PL2-6 also labels the exterior chromatin surface, covering the face of both segregating nuclei, facing the plasma membrane of the mother cell. The possible role of epichromatin in T. gondii is discussed.
authors.affiliation: Laboratorio de Parasitología Molecular, IIB-INTECH, CONICET-UNSAM, Av. Intendente Marino Km. 8·2, C.C 164, (B7130IIWA), Chascomús, Prov. Buenos Aires, Argentina.
authors.forename: Laura
authors.initials: L
authors.lastname: Vanagas
authors.forename: Maria C
authors.initials: MC
authors.lastname: Dalmasso
authors.forename: Jean F
authors.initials: JF
authors.lastname: Dubremetz
authors.forename: Enrique L
authors.initials: EL
authors.lastname: Portiansky
authors.forename: Donald E
authors.initials: DE
authors.lastname: Olins
authors.forename: Sergio O
authors.initials: SO
authors.lastname: Angel
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000378:metabolism; D000913:Antibodies, Protozoan / Q000276:immunology*; D002453:Cell Cycle; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism; D002843:Chromatin / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D004261:DNA Replication; D016054:DNA, Protozoan / Q000235:genetics / Q000378:metabolism; D000939:Epitopes / Q000276:immunology; D005347:Fibroblasts / Q000469:parasitology; D006657:Histones / Q000235:genetics / Q000378:metabolism; D006801:Humans; D051379:Mice; D009685:Nuclear Envelope / Q000235:genetics / Q000378:metabolism; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014122:Toxoplasma / Q000235:genetics* / Q000276:immunology / Q000502:physiology; D014123:Toxoplasmosis / Q000469:parasitology*
chemical_list: D000911:Antibodies, Monoclonal; D000913:Antibodies, Protozoan; D002843:Chromatin; D016054:DNA, Protozoan; D000939:Epitopes; D006657:Histones; D015800:Protozoan Proteins; D011994:Recombinant Proteins