title: Differentiation of immature DCs into endothelial-like cells in human esophageal carcinoma tissue homogenates.
abstract: We previously reported endothelial-like differentiation (ELD) of immature dendritic cells (iDCs) in the microenvironment derived from EC9706 human esophageal squamous cell carcinoma conditioned medium (CM). However, the CM is far different from the esophageal carcinoma tissue of patients. In addition, the potential role of peri-esophageal carcinoma in the ELD of iDCs is also unknown. In the present study, we showed that the tumor microenvironment derived from esophageal carcinoma homogenate promoted iDCs to differentiate from the DC pathway toward endothelial cells, while the peri-esophageal carcinoma homogenate did not have this function. During the course of ELD, ERK signaling pathway and CREB were activated. Blocking MEK, both the phosphorylation of ERK and CREB, and the ELD of iDCs were inhibited. These data suggest that esophageal carcinoma tissue, not peri-esophageal carcinoma tissue, can drive iDCs to differentiate into endothelial-like cells, instead of differentiation into mature DCs, thereby losing the ability of antigen presentation.
authors.affiliation: Department of Pathophysiology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Lu
authors.forename: Ruihua
authors.initials: R
authors.lastname: Bai
authors.forename: Zhenzhu
authors.initials: Z
authors.lastname: Qin
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhang
authors.forename: Yanan
authors.initials: Y
authors.lastname: Jiang
authors.forename: Hongyan
authors.initials: H
authors.lastname: Yang
authors.forename: Youtian
authors.initials: Y
authors.lastname: Huang
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.forename: Mingyao
authors.initials: M
authors.lastname: Zhao
authors.forename: Ziming
authors.initials: Z
authors.lastname: Dong
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000201:enzymology / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002454:Cell Differentiation / Q000502:physiology; D002460:Cell Line; D017362:Cyclic AMP Response Element-Binding Protein / Q000235:genetics / Q000378:metabolism; D003713:Dendritic Cells / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D042783:Endothelial Cells / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D004938:Esophageal Neoplasms / Q000201:enzymology / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D000077277:Esophageal Squamous Cell Carcinoma; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D020935:MAP Kinase Signaling System; D010766:Phosphorylation; D015398:Signal Transduction
chemical_list: D014408:Biomarkers, Tumor; C115163:CREB1 protein, human; D017362:Cyclic AMP Response Element-Binding Protein