title: Evaluation of the impact of dietary petroselinic acid on the growth performance, fatty acid composition, and efficacy of long chain-polyunsaturated fatty acid biosynthesis of farmed Nile tilapia.
abstract: The present study aimed to investigate the potential role of dietary petroselinic acid (PSA) in enhancing the n-3 long-chain polyunsaturated fatty acid (LC-PUFA) content in fish tissues. Three isolipidic casein-based diets were formulated to comprise graded levels of PSA (0, 10, or 20% of total fatty acid) with the incremented inclusion of coriander seed oil. Fish growth and nutrient digestibility were not significantly (P > 0.05) influenced by dietary PSA level. In general, dietary PSA affected the fatty acid composition of tilapia tissues and whole-body, which reflected dietary fatty acid ratios. Dietary PSA significantly (P < 0.05) increased β-oxidation, particularly on α-linolenic acid (18:3n-3) and linoleic acid (18:2n-6). This study provided evidence that PSA, a pseudoproduct mimicking the structure of 18:3n-6, did reduce Δ-6 desaturation on 18:2n-6 but, contrary to popular speculation, did not stimulate more Δ-6 desaturase activity on 18:3n-3. The overall Δ-6 desaturase enzyme activity may be suppressed at high dietary levels of PSA. Nevertheless, the n-3 and n-6 LC-PUFA biosynthesis was not significantly inhibited by dietary PSA, indicating that the bioconversion efficiency is not modulated only by Δ-6 desaturase. The deposition of n-3 LC-PUFA in liver and fillet lipids was higher in fish fed PSA-supplemented diets.
authors.affiliation: Fish Nutrition Laboratory, School of Biological Sciences, Universiti Sains Malaysia , Penang 11800, Malaysia.
authors.forename: Chaiw-Yee
authors.initials: CY
authors.lastname: Teoh
authors.forename: Wing-Keong
authors.initials: WK
authors.lastname: Ng
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D053180:Animal Nutrition Sciences / Q000379:methods*; D000818:Animals; D017756:Aquaculture; D023681:Cichlids / Q000254:growth & development* / Q000378:metabolism*; D019587:Dietary Supplements / Q000032:analysis; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis* / Q000737:chemistry; D009829:Oleic Acids / Q000032:analysis / Q000378:metabolism*
chemical_list: D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D009829:Oleic Acids; C008820:petroselinic acid