title: Temporal analysis of protein lysine acetylation during adipocyte differentiation.
abstract: The post-translational modification of protein by acetylation has been emerging as a prevalent modification in enzymes that catalyze intermediary metabolism. However, the dynamics of protein acetylation during adipocyte differentiation that involves a major shift in cellular metabolism is not known. In this study, we investigated the temporal changes in acetylation during adipocyte differentiation. Almost all acetylated proteins identified showed a sequential change in acetylation during the differentiation process. While the majority of the acetylated proteins showed a sequential upregulation during adipocyte differentiation, in a few proteins a sequential downregulation of protein acetylation was also observed. Our findings suggest that a wide-ranging temporal change in protein acetylation occurs during adipocyte differentiation including differentially expressed proteins signifying an important role in adipocyte differentiation.
authors.affiliation: Department of Internal Medicine; University of Manitoba; Winnipeg, MB Canada.
authors.forename: Zuyuan
authors.initials: Z
authors.lastname: Xu
authors.forename: Sudharsana Rao
authors.initials: SR
authors.lastname: Ande
authors.forename: Suresh
authors.initials: S
authors.lastname: Mishra
keywords: 2-D electrophoresis; 3T3-L1; adipogenesis; mass spectrometry; post-translational modification; ubiquitylation