title: Synthesis and characterization of chitosan-multiwalled carbon nanotubes/hydroxyapatite nanocomposites for bone tissue engineering.
abstract: Chitosan-multiwalled carbon nanotubes/hydroxyapatite nanocomposites were synthesized by a novel in situ precipitation method. The electrostatic adsorption between multiwalled carbon nanotubes and chitosan was investigated and explained by Fourier transform infrared spectroscopy analysis. Morphology studies showed that uniform distribution of hydroxyapatite particles and multiwalled carbon nanotubes in the polymer matrix was observed. In chitosan-multiwalled carbon nanotubes/hydroxyapatite nanocomposites, the diameters of multiwalled carbon nanotubes were about 10 nm. The mechanical properties of the composites were evaluated by measuring their compressive strength and elastic modulus. The elastic modulus and compressive strength increased sharply from 509.9 to 1089.1 MPa and from 33.2 to 105.5 MPa with an increase of multiwalled carbon/chitosan weight ratios from 0 to 5 %, respectively. Finally, the cell biocompatibility of the composites was tested in vitro, which showed that they have good biocompatibility. These results suggest that the chitosan-multiwalled carbon nanotubes/hydroxyapatite nanocomposites are promising biomaterials for bone tissue engineering.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.forename: Jingxiao
authors.initials: J
authors.lastname: Hu
authors.forename: Xinyu
authors.initials: X
authors.lastname: Shen
authors.forename: Hua
authors.initials: H
authors.lastname: Tong
mesh_terms: D000818:Animals; D018786:Bone Substitutes / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D001842:Bone and Bones / Q000166:cytology / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry* / Q000494:pharmacology; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D017886:Durapatite / Q000737:chemistry* / Q000494:pharmacology; D008422:Materials Testing; D051379:Mice; D008954:Models, Biological; D053761:Nanocomposites / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010006:Osteoblasts / Q000187:drug effects / Q000502:physiology; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods
chemical_list: D018786:Bone Substitutes; D020099:Coated Materials, Biocompatible; D037742:Nanotubes, Carbon; D048271:Chitosan; D017886:Durapatite