title: [Use of 2-DE and MALDI-TOF-MS to screen differentially expressed serum proteins in patients with oral lichen planus before and after hydroxychloroquine treatment].
abstract: OBJECTIVE
  To establish 2-dimensional gel electrophoresis images and compare the differences of serum proteins of oral lichen planus patients before and after hydroxychloroquine therapy.
  
  
  METHODS
  The serum of oral lichen planus patients before and after hydroxychloroquine therapy were collected, and total protein were extracted. Differential proteome profiles were established and analysed by means of 2-DE and MALDI-TOF-MS. The types and functions of protein were analyzed. SAS 9.12 software package was used for statistical analysis.
  
  
  RESULTS
  Six proteins were well characterized including plasminogen precursor,Apo A-IV precursor, C4A/C4B complement, C2 precursor, Vitamin D binding protein and hypothetical protein. The differences were statistically significant.
  
  
  CONCLUSIONS
  Plasminogen precursor, Apo A-IV precursor, C4A/C4B complement, C2 precursor, Vitamin D binding protein and hypothetical protein are differentially expressed in oral lichen planus patients before and after hydroxychloroquine therapy, but the results need to be validated by other biochemical technologies.
authors.affiliation: Department of Oral Mucosal Diseases, Nanjing Stomatological Hospital, Nanjing, Jiangsu Province, China. qwu@eyou.com
authors.forename: Hong-liu
authors.initials: HL
authors.lastname: Jiang
authors.forename: Wen-mei
authors.initials: WM
authors.lastname: Wang
authors.forename: Ning
authors.initials: N
authors.lastname: Duan
authors.forename: Wen-hui
authors.initials: WH
authors.lastname: Jiang
authors.forename: Xiang
authors.initials: X
authors.lastname: Wang
mesh_terms: D001054:Apolipoproteins A; D001798:Blood Proteins; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D006886:Hydroxychloroquine; D017676:Lichen Planus, Oral; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001054:Apolipoproteins A; D001798:Blood Proteins; C022205:apolipoprotein A-IV; D006886:Hydroxychloroquine