title: Late adherent human bone marrow stromal cells form bone and restore the hematopoietic microenvironment in vivo.
abstract: Bone marrow stromal cells (BMSCs) are a valuable resource for skeletal regenerative medicine because of their osteogenic potential. In spite of the very general term "stem cell," this population of cells is far from homogeneous, and different BMSCs clones have greatly different phenotypic properties and, therefore, potentially different therapeutic potential. Adherence to a culture flask surface is a primary defining characteristic of BMSCs. We hypothesized that based on the adherence time we could obtain an enriched population of cells with a greater therapeutic potential. We characterized two populations of bone marrow-derived cells, those that adhered by three days (R-cells) and those that did not adhere by three days but did by six days (L-cells). Clones derived from L-cells could be induced into adipogenic, chondrogenic, and osteogenic differentiation in vitro. L-cells appeared to have greater proliferative capacity, as manifested by larger colony diameter and clones with higher CD146 expression. Only clones from L-cells developed bone marrow stroma in vivo. We conclude that the use of late adherence of BMSCs is one parameter that can be used to enrich for cells that will constitute a superior final product for cell therapy in orthopedics.
authors.affiliation: Clinical and Basic Research Division, National Institute of Traumatology and Orthopaedics, Avenida Brasil 500, 20940-070 Rio de Janeiro, RJ, Brazil. vvianna@into.saude.gov.br
authors.forename: Verônica Fernandes
authors.initials: VF
authors.lastname: Vianna
authors.forename: Danielle Cabral
authors.initials: DC
authors.lastname: Bonfim
authors.forename: Amanda Dos Santos
authors.initials: Ados S
authors.lastname: Cavalcanti
authors.forename: Marco Cury
authors.initials: MC
authors.lastname: Fernandes
authors.forename: Suzana Assad
authors.initials: SA
authors.lastname: Kahn
authors.forename: Priscila Ladeira
authors.initials: PL
authors.lastname: Casado
authors.forename: Inayá Correa
authors.initials: IC
authors.lastname: Lima
authors.forename: Samuel S
authors.initials: SS
authors.lastname: Murray
authors.forename: Elsa J Brochmann
authors.initials: EJ
authors.lastname: Murray
authors.forename: Maria Eugenia Leite
authors.initials: ME
authors.lastname: Duarte
mesh_terms: D000328:Adult; D051929:CD146 Antigen / Q000096:biosynthesis; D002448:Cell Adhesion / Q000235:genetics*; D002454:Cell Differentiation; D019070:Cell Lineage / Q000235:genetics; D002478:Cells, Cultured; D005260:Female; D005347:Fibroblasts / Q000166:cytology; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008875:Middle Aged; D010012:Osteogenesis; D044968:Regenerative Medicine; D055153:Stem Cell Niche
chemical_list: D051929:CD146 Antigen; C497902:MCAM protein, human