title: Preliminary X-ray crystallographic studies of an N-terminal domain of unknown function from a putative glycosyltransferase from Streptococcus parasanguinis.
abstract: Serine-rich repeat glycoproteins (SRRPs) belong to a growing family of bacterial adhesins; they play important roles in bacterial virulence. Fap1, the first SRRP protein to be identified, is glycosylated; while the first two steps of its glycosylation have been determined, the remaining glycosylation steps are unknown. In a search for proteins that might be relevant to the glycosylation of Fap1, a putative glycosyltransferase (GalT1) from Streptococcus parasanguinis was identified. GalT1 possesses a domain of unknown function at the N-terminus. This domain is highly conserved in bacteria and is a member of a broad superfamily. However, the structure of this domain has not been determined. Here, the conditions used to produce a recombinant version of this protein domain and to grow protein crystals are reported. The crystals obtained belonged to space group C2, with unit-cell parameters a = 71.0, b = 45.1, c = 78.6 Å, β = 109.6°, and diffracted to 1.55 Å resolution at a synchrotron X-ray source. This domain does not share sequence identity with proteins of known structures above a level of 12%.
authors.affiliation: Department of Pediatric Dentistry, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.forename: Fan
authors.initials: F
authors.lastname: Zhu
authors.forename: Lei
authors.initials: L
authors.lastname: Ding
authors.forename: Meixian
authors.initials: M
authors.lastname: Zhou
authors.forename: Ren
authors.initials: R
authors.lastname: Wu
authors.forename: Hui
authors.initials: H
authors.lastname: Wu
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D018360:Crystallography, X-Ray; D016695:Glycosyltransferases / Q000737:chemistry* / Q000302:isolation & purification; D017434:Protein Structure, Tertiary; D013291:Streptococcus / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D016695:Glycosyltransferases
keywords: DUF1792; Streptococcus parasanguinis; glycosyltransferase