title: Differences in the effect of phosphatidylinositol 4,5-bisphosphate on the hydrolytic and transphosphatidylation activities of membrane-bound phospholipase D from poppy seedlings.
abstract: The hydrolytic activity of phospholipase D (PLD) yielding phosphatidic acid from phosphatidylcholine and other glycerophospholipids is known to be involved in many cellular processes. In contrast, it is not clear whether the competitive transphosphatidylation activity of PLD catalyzing the head group exchange of phospholipids has a natural function. In poppy seedlings (Papaver somniferum L.) where lipid metabolism and alkaloid synthesis are closely linked, five isoenzymes with different substrate and hydrolysis/transphosphatidylation selectivities have been detected hitherto. A membrane-bound PLD, found in microsomal fractions of poppy seedlings, is active at micromolar concentrations of Ca(2+) ions and needs phosphatidylinositol 4,5-bisphosphate (PIP2) as effector in the hydrolysis of phosphatidylcholine (PC). The optimum PIP2 concentration at 1.2 mol% of the concentration of the substrate PC indicates a specific activation effect. Transphosphatidylation with glycerol, ethanolamine, l-serine, or myo-inositol as acceptor alcohols is also activated by PIP2, however, with an optimum concentration at 0.6-0.9 mol%. In contrast to hydrolysis, a basic transphosphatidylation activity occurs even in the absence of PIP2, suggesting a different fine-tuning of the two competing reactions.
authors.affiliation: Department of Cell and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University, Kalinciakova 8, SK-83232 Bratislava, Slovakia.
authors.forename: Marek
authors.initials: M
authors.lastname: Oblozinsky
authors.forename: Lydia
authors.initials: L
authors.lastname: Bezakova
authors.forename: Johanna
authors.initials: J
authors.lastname: Mansfeld
authors.forename: Ingo
authors.initials: I
authors.lastname: Heilmann
authors.forename: Renate
authors.initials: R
authors.lastname: Ulbrich-Hofmann
mesh_terms: D006868:Hydrolysis / Q000187:drug effects; D010207:Papaver / Q000187:drug effects* / Q000201:enzymology*; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000494:pharmacology*; D010739:Phospholipase D / Q000378:metabolism*; D036226:Seedlings / Q000187:drug effects* / Q000201:enzymology*
chemical_list: D019269:Phosphatidylinositol 4,5-Diphosphate; D010739:Phospholipase D