title: Expression of Clostridium perfringens epsilon-beta fusion toxin gene in E. coli and its immunologic studies in mouse.
abstract: Clostridium perfringens is an anaerobic spore-forming, pathogenic bacterium that is responsible for severe diseases in humans and livestock. In the present study, an epsilon-beta fusion toxin was expressed as a soluble protein in E. coli and the recombinant cell lysate was used for immunization studies in mouse. Potency of the toxin (as an antigen) induced 6 and 10IU/ml of epsilon and beta anti-toxin in rabbit, respectively. These titers were higher than the minimum level required by the European Pharmacopoeia for epsilon and beta toxins. Experimental challenge with the recombinant fusion toxoid revealed that it could protect mice against C. perfringens epsilon and beta toxins. Toxicity of the fusion toxin was studied by histopathological findings, which were the same as the native toxins. In conclusion, E. coli is a suitable expression host for immunogenic epsilon-beta fusion toxin of C. perfringens.
authors.affiliation: Department of Anaerobic Bacterial Vaccine Research and Production, Razi Vaccine and Serum Research Institute, Karaj, Iran. r.pilehchian@rvsri.ac.ir
authors.forename: Reza
authors.initials: R
authors.lastname: Pilehchian Langroudi
authors.forename: Mehdi
authors.initials: M
authors.lastname: Shamsara
authors.forename: Khosrow
authors.initials: K
authors.lastname: Aghaiypour
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000276:immunology; D001427:Bacterial Toxins / Q000235:genetics / Q000276:immunology*; D003015:Clostridium Infections / Q000276:immunology / Q000473:pathology; D003016:Clostridium perfringens / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D009500:Neutralization Tests; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000276:immunology
chemical_list: D000942:Antigens, Bacterial; D001427:Bacterial Toxins; C031884:CPB protein, Clostridium perfringens; C039811:Clostridium perfringens epsilon-toxin; D011993:Recombinant Fusion Proteins