title: [Dynamic changes of cellular HIV-1 DNA quantification during highly active antiretroviral therapy in Chinese HIV infected individuals].
abstract: OBJECTIVE
  To observe the dynamic changes of 3 types of viral reservoir cells (NK cells, T lymphocytes and monocytes), and its relationship with treatment effect in Chinese HIV-1 infected patients receiving highly active antiretroviral treatment (HAART) for 2 years.
  
  
  METHODS
  A total of 40 chronic HIV-1-infected adults who initiated HAART were enrolled in this study and followed up for 2 years. Peripheral whole blood was obtained from each patient at baseline (0 month), 6, 12, 18 and 24 months. Real-time fluorescent quantitative PCR was used to detect the HIV-1 RNA in the plasma and HIV-1 DNA in NK cells, T lymphocytes and monocytes. All the data were statistically analyzed.
  
  
  RESULTS
  CD4 count increased with the decrease of the viral load during HAART. After HAART initiation, HIV-1 DNA showed a significant decrease in NK cells, T lymphocytes and monocytes. The HIV-1 DNA from T lymphocytes, NK cells and monocytes correlated positively with the HIV- 1 RNA (P<0.05) while NK cells and T lymphocytes correlated negatively with CD4+ T cell count. However we did not find significant correlation between CD4+ T cell count and HIV-1 DNA in monocytes at the baseline of HAART.
  
  
  CONCLUSIONS
  This study found that NK cell was an important HIV cellular reservoir besides T lymphocytes and monocytes. T lymphocytes may be the main long lasting HIV reservoir. HIV-1 proviral DNA may play an important role in the efficacy of treatment and monitoring the disease progression.
authors.affiliation: Department of Clinical Laboratory, Xiangya Hospital, Central South University, Changsha 410008,China.
authors.forename: Xia
authors.initials: X
authors.lastname: Chen
authors.forename: Yuhuang
authors.initials: Y
authors.lastname: Zheng
authors.forename: Diallo
authors.initials: D
authors.lastname: Mamadou
authors.forename: Yan
authors.initials: Y
authors.lastname: He
authors.forename: Huaying
authors.initials: H
authors.lastname: Zhou
authors.forename: Zi
authors.initials: Z
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: Luo
authors.forename: Bo
authors.initials: B
authors.lastname: He
authors.forename: Mei
authors.initials: M
authors.lastname: He
authors.forename: Yunhai
authors.initials: Y
authors.lastname: Yao
mesh_terms: D000328:Adult; D023241:Antiretroviral Therapy, Highly Active; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D015658:HIV Infections / Q000188:drug therapy* / Q000821:virology; D015497:HIV-1 / Q000235:genetics; D006801:Humans; D007694:Killer Cells, Natural / Q000821:virology*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000821:virology; D012367:RNA, Viral / Q000097:blood; D013601:T-Lymphocytes / Q000821:virology; D019562:Viral Load
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral