title: Circulating miRNAs as potential marker for pulmonary hypertension.
abstract: MircoRNAs (miRNAs) are small non-coding RNAs that govern the gene expression and, play significant role in the pathogenesis of heart failure. The detection of miRNAs in circulation of pulmonary hypertensive (PH) human subjects remains elusive. In the current study, we determined the pattern of miRNAs of mild-to-severe human PH subjects and, compared them with the control subjects by miRNA array. Blood was obtained using fluoroscopic and waveform guided catheterization from the distal (pulmonary artery) port of the catheter. A total 40 human subjects were included in the study and, the degree of PH was determined by mean pulmonary arterial pressure. Among several miRNAs in the array, we validated 14 miRNAs and, the data were consistent with the array profile. We identified several novel downregulated miRNAs (miR-451, miR-1246) and upregulated miRNAs (miR-23b, miR-130a and miR-191) in the circulation of PH subjects. Our study showed novel set of miRNAs which are dysregulated in PH and, are directly proportional to the degree of PH. These miRNAs may be considered as potential biomarker for early detection of PH.
authors.affiliation: Division of Molecular Cardiology, Department of Medicine, College of Medicine, Texas A&M Health Science Center, Scott & White, Central Texas Veterans Health Care System, Temple, Texas, United States of America.
authors.forename: Chuanyu
authors.initials: C
authors.lastname: Wei
authors.forename: Heather
authors.initials: H
authors.lastname: Henderson
authors.forename: Christopher
authors.initials: C
authors.lastname: Spradley
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Il-Kwon
authors.initials: IK
authors.lastname: Kim
authors.forename: Sandeep
authors.initials: S
authors.lastname: Kumar
authors.forename: Nayeon
authors.initials: N
authors.lastname: Hong
authors.forename: Alejandro C
authors.initials: AC
authors.lastname: Arroliga
authors.forename: Sudhiranjan
authors.initials: S
authors.lastname: Gupta
mesh_terms: D015415:Biomarkers / Q000097:blood; D058621:Blood Buffy Coat / Q000378:metabolism; D016022:Case-Control Studies; D015536:Down-Regulation; D005260:Female; D015870:Gene Expression; D006801:Humans; D006976:Hypertension, Pulmonary / Q000097:blood*; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D012727:Sex Characteristics
chemical_list: D015415:Biomarkers; D035683:MicroRNAs