title: Development and laboratory evaluation of chemically-based baited ovitrap for the monitoring of Aedes aegypti.
abstract: Aedes (Stegomyia) aegypti is considered to be the most important dengue vector worldwide. Studies were conducted to design and evaluate a chemically-based baited ovitrap for monitoring Ae. aegypti under laboratory conditions. Several known chemical attractants and three types of ovitraps (ovitraps A, B, and C) were evaluated throughout the oviposition bioassays. Oviposition responses of gravid female Ae. aegypti were evaluated to n-heneicosane, 3-methylindole (skatole), 4-methylphenol (p-cresol), and phenol. Female Ae. aegypti were attracted to all the evaluated compounds. Among them, n-heneicosane at a concentration of 10 ppm (mg/l), skatole from 50 to 1000 ppm, p-cresol at 100 ppm, and phenol at 50 ppm showed a significant positive oviposition response. A blend of the four chemical attractants increased the oviposition response; 67% of the eggs were deposited in the treatment compared to the control. Female Ae. aegypti were signiﬁcantly more attracted to ovitrap A loaded with the four-component synthetic blend compared to the standard ovitrap in the oviposition bioassays. The compound used in ovitrap A retained its attractant property for up to three days. The chemically-based baited ovitrap may be considered as an option to be integrated during the monitoring of dengue virus vectors in México.
authors.affiliation: Centro Regional de Investigación en Salud Pública, Instituto Nacional de Salud Pública, CP 30700 Tapachula, Chiapas, México.
authors.forename: Carlos M
authors.initials: CM
authors.lastname: Baak-Baak
authors.forename: Américo D
authors.initials: AD
authors.lastname: Rodríguez-Ramírez
authors.forename: Julián E
authors.initials: JE
authors.lastname: García-Rejón
authors.forename: Silvany
authors.initials: S
authors.lastname: Ríos-Delgado
authors.forename: José L
authors.initials: JL
authors.lastname: Torres-Estrada
mesh_terms: D000330:Aedes; D000818:Animals; D001681:Biological Assay / Q000379:methods*; D003408:Cresols / Q000494:pharmacology; D005260:Female; D007299:Insect Bites and Stings; D007753:Laboratories; D008297:Male; D010058:Oviposition / Q000187:drug effects; D019800:Phenol / Q000494:pharmacology; D012862:Skatole / Q000494:pharmacology
chemical_list: D003408:Cresols; C032538:4-cresol; D019800:Phenol; D012862:Skatole