title: Oleanane-triterpenoids from Panax stipuleanatus inhibit NF-κB.
abstract: In continuation of our research to find biological components from Panax stipuleanatus, four oleanane-type triterpenes (12 to 15) were isolated successively. Fifteen oleanane-type saponins (1 to 15) were evaluated for nuclear factor (NF)-κB activity using a luciferase reporter gene assay in HepG2 cells. Compounds 6 to 11 inhibited NF-κB, with IC50 values between 3.1 to 18.9 μM. The effects on inducible nitric oxide synthase and cyclooxygenase-2 by compounds 8, 10, and 11 were also examined using reverse transcription-polymerase chain reaction. Three compounds (8, 10, and 11) inhibited NF-κB activity by reducing the concentration of inflammatory factors in HepG2 cells.
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon 305-764, Korea ; Herbal Medicine Improvement Research Center, Korea Institute of Oriental Medicine, Daejeon 305-811, Korea.
authors.forename: Chun
authors.initials: C
authors.lastname: Liang
authors.forename: Yan
authors.initials: Y
authors.lastname: Ding
authors.forename: Seok Bean
authors.initials: SB
authors.lastname: Song
authors.forename: Jeong Ah
authors.initials: JA
authors.lastname: Kim
authors.forename: Nguyen Manh
authors.initials: NM
authors.lastname: Cuong
authors.forename: Jin Yeul
authors.initials: JY
authors.lastname: Ma
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
keywords: Nuclear factor-κB; Oleanane-type triterpenoid; Panax ginseng; Panax stipuleanatus