title: Eutopic and ectopic stromal cells from patients with endometriosis exhibit differential invasive, adhesive, and proliferative behavior.
abstract: OBJECTIVE
  To study immunophenotype, differential proliferation capacity, invasiveness, adhesion, and cytokine production in ectopic and eutopic endometrial stromal cells (EESCs and EuESCs) from patients with endometriosis.
  
  
  METHODS
  In vitro study.
  
  
  METHODS
  Academic research center.
  
  
  METHODS
  Patients with ovarian endometriosis (endometrioma) and nonendometriotic controls.
  
  
  METHODS
  None.
  
  
  METHODS
  EESCs and EuESCs from 25 patients with endometrioma and ESCs from 20 nonendometriotic controls (CESCs) were isolated, and their immunophenotype, proliferation, invasion, adhesion, and cytokine production were assessed and compared.
  
  
  RESULTS
  Isolated ESCs from all three sources expressed markers specific for cells of mesenchymal origin but were negative for hematopoietic markers. EESCs exhibited a significantly lower proliferation rate in fibronectin-coated plates and less invasive capacity compared with CESCs or EuESCs. Among all stromal cell groups studied, EuESCs showed the highest invasive behavior. EESCs adhered more firmly to extracellular matrix than EuESCs or CESCs in all time intervals examined. The levels of interleukin (IL) -6 and IL-8 production by EESCs were significantly higher compared with those of EuESCs or CESCs.
  
  
  CONCLUSIONS
  The results of the present study demonstrated that retrograde menstruation alone does not account for the pathogenesis of endometriosis as eutopic and ectopic counterparts of ESCs from patients with endometriosis exhibit differential invasive, adhesive, and proliferative behavior.
authors.affiliation: Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Ali-Akbar
authors.initials: AA
authors.lastname: Delbandi
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Mahmoudi
authors.forename: Adel
authors.initials: A
authors.lastname: Shervin
authors.forename: Elham
authors.initials: E
authors.lastname: Akbari
authors.forename: Mahmood
authors.initials: M
authors.lastname: Jeddi-Tehrani
authors.forename: Mojtaba
authors.initials: M
authors.lastname: Sankian
authors.forename: Somayeh
authors.initials: S
authors.lastname: Kazemnejad
authors.forename: Amir-Hassan
authors.initials: AH
authors.lastname: Zarnani
mesh_terms: D000328:Adult; D002448:Cell Adhesion; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D002828:Choristoma / Q000473:pathology*; D016207:Cytokines / Q000378:metabolism; D004715:Endometriosis / Q000473:pathology*; D004717:Endometrium; D005260:Female; D006801:Humans; D008875:Middle Aged; D017154:Stromal Cells / Q000473:pathology*; D055815:Young Adult
chemical_list: D016207:Cytokines
keywords: Endometrioma; adhesion; cytokine; eutopic; immunophenotype; invasion; proliferation