title: Regulation of centrosome movements by numb and the collapsin response mediator protein during Drosophila sensory progenitor asymmetric division.
abstract: Asymmetric cell division generates cell fate diversity during development and adult life. Recent findings have demonstrated that during stem cell divisions, the movement of centrosomes is asymmetric in prophase and that such asymmetry participates in mitotic spindle orientation and cell polarization. Here, we have investigated the dynamics of centrosomes during Drosophila sensory organ precursor asymmetric divisions and find that centrosome movements are asymmetric during cytokinesis. We demonstrate that centrosome movements are controlled by the cell fate determinant Numb, which does not act via its classical effectors, Sanpodo and α-Adaptin, but via the Collapsin Response Mediator Protein (CRMP). Furthermore, we find that CRMP is necessary for efficient Notch signalling and that it regulates the duration of the pericentriolar accumulation of Rab11-positive endosomes, through which the Notch ligand, Delta is recycled. Our work characterizes an additional mode of asymmetric centrosome movement during asymmetric divisions and suggests a model whereby the asymmetry in centrosome movements participates in differential Notch activation to regulate cell fate specification.
authors.affiliation: Polarity, Division and Morphogenesis Team, Institut Curie, CNRS UMR 3215, INSERM U934, 26 rue d'Ulm, 75248 Paris Cedex 05, France.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Jauffred
authors.forename: Flora
authors.initials: F
authors.lastname: Llense
authors.forename: Bernhard
authors.initials: B
authors.lastname: Sommer
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Wang
authors.forename: Charlotte
authors.initials: C
authors.lastname: Martin
authors.forename: Yohanns
authors.initials: Y
authors.lastname: Bellaiche
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics / Q000502:physiology; D018385:Centrosome / Q000378:metabolism*; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D047468:Immunoprecipitation; D007605:Juvenile Hormones / Q000235:genetics / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D012679:Sense Organs / Q000166:cytology*; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*
chemical_list: C576787:CRMP protein, Drosophila; D029721:Drosophila Proteins; D007605:Juvenile Hormones; D009419:Nerve Tissue Proteins; C060606:numb protein, Drosophila
keywords: Asymmetric cell division; Centrosome; Cytokinesis; Drosophila; Notch signalling; Rab11