title: The ameliorative effect of sophoricoside on mast cell-mediated allergic inflammation in vivo and in vitro.
abstract: Sophoricoside exhibits numerous pharmacological effects, including anti- inflammatory and anti-cancer actions, yet the exact mechanism that accounts for the anti-allergic effects of sophoricoside is not completely understood. The aim of the present study was to elucidate whether and how sophoricoside modulates the mast cell-mediated allergic inflammation in vitro and in vivo. We investigated the pharmacological effects of sophoricoside on both compound 48/80 or histamine-induced scratching behaviors and 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis in mice. Additionally, to find a possible explanation for the anti-inflammatory effects of sophoricoside, we evaluated the effects of sophoricoside on the production of histamine and inflammatory cytokines and activation of nuclear factor-κB (NF-κB) and caspase-1 in phorbol 12-myristate 13-acetate plus calcium ionophore A23187 (PMACI)-stimulated human mast cells (HMC-1). The finding of this study demonstrated that sophoricoside reduced compound 48/80 or histamine-induced scratching behaviors and DNCB-induced atopic dermatitis in mice. Additionally, sophoricoside inhibited the production of inflammatory cytokines as well as the activation of NF-κB and caspase-1 in stimulated HMC-1. Collectively, the findings of this study provide us with novel insights into the pharmacological actions of sophoricoside as a potential molecule for use in the treatment of allergic inflammation diseases.
authors.affiliation: Department of Cosmeceutical Science, Daegu Hanny University, Yugok-dong, Kyungsan 712-715, Korea. jyum@khu.ac.kr.
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Kim
authors.forename: Gil-Yong
authors.initials: GY
authors.lastname: Lee
authors.forename: Ji-Wook
authors.initials: JW
authors.lastname: Jung
authors.forename: Sa-Rang
authors.initials: SR
authors.lastname: Oh
authors.forename: Eun-Mi
authors.initials: EM
authors.lastname: Ahn
authors.forename: Sung-Hoon
authors.initials: SH
authors.lastname: Kim
authors.forename: Seung-Heon
authors.initials: SH
authors.lastname: Hong
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Um
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001578:Benzopyrans / Q000494:pharmacology*; D000001:Calcimycin / Q000494:pharmacology; D061207:Calcium Ionophores / Q000494:pharmacology; D002273:Carcinogens / Q000494:pharmacology; D020170:Caspase 1 / Q000378:metabolism; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism; D003876:Dermatitis, Atopic / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D004137:Dinitrochlorobenzene / Q000009:adverse effects / Q000494:pharmacology; D006632:Histamine / Q000378:metabolism; D006801:Humans; D007509:Irritants / Q000009:adverse effects / Q000494:pharmacology; D008297:Male; D008407:Mast Cells / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D008813:Mice, Inbred ICR; D016328:NF-kappa B / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology
chemical_list: D000893:Anti-Inflammatory Agents; D001578:Benzopyrans; D061207:Calcium Ionophores; D002273:Carcinogens; D016207:Cytokines; D004137:Dinitrochlorobenzene; D007509:Irritants; D016328:NF-kappa B; C120642:sophoricoside; D000001:Calcimycin; D006632:Histamine; D020170:Caspase 1; D013755:Tetradecanoylphorbol Acetate