title: Comparison of global gene expression of gastric cardia and noncardia cancers from a high-risk population in china.
abstract: OBJECTIVE
  To profile RNA expression in gastric cancer by anatomic subsites as an initial step in identifying molecular subtypes and providing targets for early detection and therapy.
  
  
  METHODS
  We performed transcriptome analysis using the Affymetrix GeneChip U133A in gastric cardia adenocarcinomas (n = 62) and gastric noncardia adenocarcinomas (n = 72) and their matched normal tissues from patients in Shanxi Province, and validated selected dysregulated genes with additional RNA studies. Expression of dysregulated genes was also related to survival of cases.
  
  
  RESULTS
  Principal Component Analysis showed that samples clustered by tumor vs. normal, anatomic location, and histopathologic features. Paired t-tests of tumor/normal tissues identified 511 genes whose expression was dysregulated (P<4.7E-07 and at least two-fold difference in magnitude) in cardia or noncardia gastric cancers, including nearly one-half (n = 239, 47%) dysregulated in both cardia and noncardia, one-fourth dysregulated in cardia only (n = 128, 25%), and about one-fourth in noncardia only (n = 144, 28%). Additional RNA studies confirmed profiling results. Expression was associated with case survival for 20 genes in cardia and 36 genes in noncardia gastric cancers.
  
  
  CONCLUSIONS
  The dysregulated genes identified here represent a comprehensive starting point for future efforts to understand etiologic heterogeneity, develop diagnostic biomarkers for early detection, and test molecularly-targeted therapies for gastric cancer.
authors.affiliation: Genetic Epidemiology Branch, Division of Cancer Epidemiology and Genetics, NCI, Bethesda, Maryland, USA.
authors.forename: Gangshi
authors.initials: G
authors.lastname: Wang
authors.forename: Nan
authors.initials: N
authors.lastname: Hu
authors.forename: Howard H
authors.initials: HH
authors.lastname: Yang
authors.forename: Lemin
authors.initials: L
authors.lastname: Wang
authors.forename: Hua
authors.initials: H
authors.lastname: Su
authors.forename: Chaoyu
authors.initials: C
authors.lastname: Wang
authors.forename: Robert
authors.initials: R
authors.lastname: Clifford
authors.forename: Erica M
authors.initials: EM
authors.lastname: Dawsey
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Li
authors.forename: Ti
authors.initials: T
authors.lastname: Ding
authors.forename: Xiao-You
authors.initials: XY
authors.lastname: Han
authors.forename: Carol
authors.initials: C
authors.lastname: Giffen
authors.forename: Alisa M
authors.initials: AM
authors.lastname: Goldstein
authors.forename: Philip R
authors.initials: PR
authors.lastname: Taylor
authors.forename: Maxwell P
authors.initials: MP
authors.lastname: Lee
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis / Q000235:genetics / Q000401:mortality / Q000473:pathology; D002299:Cardia; D002681:China; D015536:Down-Regulation / Q000235:genetics; D055088:Early Detection of Cancer / Q000379:methods; D005260:Female; D015870:Gene Expression / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D008297:Male; D008875:Middle Aged; D012313:RNA / Q000235:genetics; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000401:mortality / Q000473:pathology*; D059467:Transcriptome / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics
chemical_list: D012313:RNA