title: Isolation of nematicidal triterpenoid saponins from Pulsatilla koreana root and their activities against Meloidogyne incognita.
abstract: Pulsatilla koreana, a species endemic to Korea, is an important herb used in traditional medicine to treat amoebic dysentery and malaria. In the present study, 23 oleanane-type triterpenoid saponins 1-23 and eight lupane-type triterpenoid saponins 24-31 were isolated from the roots of P. koreana. Their structures were elucidated on the basis of spectroscopic data. The methanol extract and isolated compounds were next assessed for nematicidal activity against the root-knot nematode (Meloidogyne incognita). The methanol extract showed strong nematicidal activity after 48 h, with a LC₅₀ value of 92.8 μg/mL. Compounds 2, 5, 9, 20, and 21 showed significant effects, with LC₅₀ values ranging from 70.1 to 94.7 μg/mL after 48 h. These results suggest that triterpenoid saponins from P. koreana should be explored as potential natural nematicides for developing new agents to control root-knot nematode disease.
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon 305-764, Korea.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Ya Nan
authors.initials: YN
authors.lastname: Sun
authors.forename: Xi Tao
authors.initials: XT
authors.lastname: Yan
authors.forename: Seo Young
authors.initials: SY
authors.lastname: Yang
authors.forename: Suk Jun
authors.initials: SJ
authors.lastname: Lee
authors.forename: Hyo Jeung
authors.initials: HJ
authors.lastname: Byun
authors.forename: Chang Sup
authors.initials: CS
authors.lastname: Moon
authors.forename: Byung Soo
authors.initials: BS
authors.lastname: Han
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
mesh_terms: D000818:Animals; D000969:Antinematodal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D007723:Korea; D018517:Plant Roots / Q000737:chemistry*; D031946:Pulsatilla / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D014431:Tylenchoidea
chemical_list: D000969:Antinematodal Agents; D012503:Saponins; D014315:Triterpenes