title: In vivo tomographic imaging of lung colonization of tumour in mouse with simultaneous fluorescence and X-ray CT.
abstract: Non-invasive in vivo imaging of diffuse and wide-spread colonization within the lungs, rather than distinct solid primary tumors, is still a challenging work. In this work, a lung colonization mouse model bearing A549 human lung tumor was simultaneously scanned by a dual-modality fluorescence molecular tomography (FMT) and X-ray computed tomography (CT) system in vivo. A two steps method which incorporates CT structural information into the FMT reconstruction procedure is employed to provide concurrent anatomical and functional information. By using the target-specific fluorescence agent, the fluorescence tomographic results show elevated fluorescence intensity deep within the lungs which is colonized with diffuse and wide-spread tumors. The results were confirmed with ex vivo fluorescence reflectance imaging and histological examination of the lung tissues. With FMT reconstruction combined with the CT information, the dual-modality FMT/micro-CT system is expected to offer sensitive and noninvasive imaging of diffuse tumor colonization within the lungs in vivo.
authors.affiliation: Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing, 100084, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.forename: Fuping
authors.initials: F
authors.lastname: Gao
authors.forename: Mengjiao
authors.initials: M
authors.lastname: Wang
authors.forename: Xu
authors.initials: X
authors.lastname: Cao
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Jianwen
authors.initials: J
authors.lastname: Luo
authors.forename: Guangzhi
authors.initials: G
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Bai
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004058:Diffusion; D005453:Fluorescence; D006801:Humans; D021621:Imaging, Three-Dimensional; D008168:Lung / Q000000981:diagnostic imaging* / Q000473:pathology*; D008175:Lung Neoplasms / Q000000981:diagnostic imaging* / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013997:Time Factors; D014057:Tomography, X-Ray Computed / Q000379:methods*
keywords: X-ray computed tomography; fluorescence molecular tomography; lung colonization; multi-modality imaging