title: Effect of Beta-glucan on intestinal anastomoses in a rat model.
abstract: OBJECTIVE
  The authors aimed to investigate the effect of β-Glucan on healing of an experimental left-sided colon anastomosis model.
  
  
  METHODS
  Twenty adult male Wistar albino rats were randomized into two groups which had colonic transection and end-to-end anastomosis. Group I (Control): anastomosis group, received no treatment (n = 10); group II, anastomosis + β-Glucan (50 mg/kg/day within seven days after surgical procedure). Bursting pressure, hydroxyproline levels and histopathological characteristics of the anastomosis were analyzed.
  
  
  RESULTS
  The average burst pressure of Groups I and II were 106.67 ± 5.00 and 148.00 ± 11.35 mmHg and hydroxyproline levels were 0.85 ± 0.14 and 1.45 ± 0.46 µg/mg, respectively. Both the burst pressure and hydroxyproline levels in group II were statistically significantly higher (p < 0.05). Histopathological examination revealed less epithelial damage in group II (p < 0.05). Though not statistically significant, less edema and damage to the submucosal-muscular layer was seen in Group II (p = 0079).
  
  
  CONCLUSIONS
  Due to significant increases in anastomotic bursting pressures and tissue hydroxyproline levels and considering the inhibitory effect of β-Glucan on epithelial damage, edema, and submucosal-muscular layer damage, β-Glucan was thought to contribute to the healing of the anastomosis.
authors.affiliation: Department of Surgery, Bozok University, Yozgat, Turkey. kasimcaglayan@hotmail.com
authors.forename: Kasim
authors.initials: K
authors.lastname: Caglayan
authors.forename: Nilsen
authors.initials: N
authors.lastname: Erdogan
authors.forename: Bahattin
authors.initials: B
authors.lastname: Avci
authors.forename: Bulent
authors.initials: B
authors.lastname: Gungor
authors.forename: Hamza
authors.initials: H
authors.lastname: Cinar
authors.forename: Nilden
authors.initials: N
authors.lastname: Arslan
mesh_terms: D000714:Anastomosis, Surgical / Q000009:adverse effects; D000818:Animals; D003106:Colon / Q000187:drug effects* / Q000473:pathology / Q000601:surgery; D004195:Disease Models, Animal; D004338:Drug Combinations; D006854:Hydrocortisone / Q000378:metabolism; D008297:Male; D011183:Postoperative Complications / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D011312:Pressure; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013529:Surgical Wound Dehiscence / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D014508:Urea / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects*; D047071:beta-Glucans / Q000494:pharmacology*
chemical_list: D004338:Drug Combinations; D047071:beta-Glucans; C010017:hydrocortisone, urea drug combination; D014508:Urea; D006854:Hydrocortisone