title: Molecular techniques for dicistrovirus detection without RNA extraction or purification.
abstract: Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines. The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.
authors.affiliation: Centre for Malaria and Tropical Diseases, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira, 100, 1349-008 Lisboa, Portugal.
authors.forename: Jailson F B
authors.initials: JF
authors.lastname: Querido
authors.forename: Jon
authors.initials: J
authors.lastname: Agirre
authors.forename: Gerardo A
authors.initials: GA
authors.lastname: Marti
authors.forename: Diego M A
authors.initials: DM
authors.lastname: Guérin
authors.forename: Marcelo Sousa
authors.initials: MS
authors.lastname: Silva
mesh_terms: D000818:Animals; D056629:Dicistroviridae / Q000235:genetics / Q000302:isolation & purification*; D005243:Feces / Q000821:virology; D007304:Insect Viruses / Q000302:isolation & purification*; D012313:RNA / Q000235:genetics; D014224:Triatoma / Q000821:virology*
chemical_list: D012313:RNA