title: In silico analysis of prion protein mutants: a comparative study by molecular dynamics approach.
abstract: Polymorphisms in the human prion proteins lead to amino acid substitutions by the conversion of PrPC to PrPSc and amyloid formation, resulting in prion diseases such as familial Creutzfeldt-Jakob disease, Gerstmann-Straussler-Scheinker disease and fatal familial insomnia. Cation-π interaction is a non-covalent binding force that plays a significant role in protein stability. Here, we employ a novel approach by combining various in silico tools along with molecular dynamics simulation to provide structural and functional insight into the effect of mutation on the stability and activity of mutant prion proteins. We have investigated impressions of prevalent mutations including 1E1S, 1E1P, 1E1U, 1E1P, 1FKC and 2K1D on the human prion proteins and compared them with wild type. Structural analyses of the models were performed with the aid of molecular dynamics simulation methods. According to our results, frequently occurred mutations were observed in conserved sequences of human prion proteins and the most fluctuation values appear in the 2K1D mutant model at around helix 4 with residues ranging from 190 to 194. Our observations in this study could help to further understand the structural stability of prion proteins.
authors.affiliation: Medical Biotechnology Division, School of Biosciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India, georgecp77@yahoo.co.in.
authors.forename: C George Priya
authors.initials: CG
authors.lastname: Doss
authors.forename: B
authors.initials: B
authors.lastname: Rajith
authors.forename: R
authors.initials: R
authors.lastname: Rajasekaran
authors.forename: Jain
authors.initials: J
authors.lastname: Srajan
authors.forename: N
authors.initials: N
authors.lastname: Nagasundaram
authors.forename: C
authors.initials: C
authors.lastname: Debajyoti
mesh_terms: D000595:Amino Acid Sequence; D002412:Cations / Q000737:chemistry; D030562:Databases, Protein; D004583:Electrons; D006801:Humans; D056004:Molecular Dynamics Simulation; D008969:Molecular Sequence Data; D009154:Mutation; D011110:Polymorphism, Genetic; D011328:Prions / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D017433:Protein Structure, Secondary; D013816:Thermodynamics
chemical_list: D002412:Cations; D011328:Prions