title: Genomic region operation kit for flexible processing of deep sequencing data.
abstract: Computational analysis of data produced in deep sequencing (DS) experiments is challenging due to large data volumes and requirements for flexible analysis approaches. Here, we present a mathematical formalism based on set algebra for frequently performed operations in DS data analysis to facilitate translation of biomedical research questions to language amenable for computational analysis. With the help of this formalism, we implemented the Genomic Region Operation Kit (GROK), which supports various DS-related operations such as preprocessing, filtering, file conversion, and sample comparison. GROK provides high-level interfaces for R, Python, Lua, and command line, as well as an extension C++ API. It supports major genomic file formats and allows storing custom genomic regions in efficient data structures such as red-black trees and SQL databases. To demonstrate the utility of GROK, we have characterized the roles of two major transcription factors (TFs) in prostate cancer using data from 10 DS experiments. GROK is freely available with a user guide from >http://csbi.ltdk.helsinki.fi/grok/.
authors.affiliation: Biomedicine, Biochemistry and Developmental Biology, Biomedicum Helsinki, University of Helsinki, Helsinki, Finland.
authors.forename: Kristian
authors.initials: K
authors.lastname: Ovaska
authors.forename: Lauri
authors.initials: L
authors.lastname: Lyly
authors.forename: Biswajyoti
authors.initials: B
authors.lastname: Sahu
authors.forename: Olli A
authors.initials: OA
authors.lastname: Jänne
authors.forename: Sampsa
authors.initials: S
authors.lastname: Hautaniemi
mesh_terms: D019295:Computational Biology / Q000379:methods*; D020869:Gene Expression Profiling; D015894:Genome, Human; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D008297:Male; D008957:Models, Genetic; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism; D012984:Software; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D014157:Transcription Factors