title: Preparation of a dibenzylated 1,4-diazacyclohexane-derived strong anion-exchange stationary phase.
abstract: This paper reports the preparation of a novel, silica-based, strong anion-exchange stationary phase from a 1,4-diazacyclohexane derivative. To prepare the difunctional strong anion-exchange stationary phase, activated silica beads were first bonded with 3-chloropropyltriethoxysilane and then reacted with 1-methylpiperazine followed by benzyl chloride. The silica beads, the strong anion-exchange stationary phase and its precusors were instrumentally characterized. Aromatic acids were separated with non-aqueous anion-exchange chromatography. After elution with eluant prepared in mixed solvents of water and methanol, the resulting 1,4-diazacyclohexane-derived, difunctional, strong anion-exchange stationary phase exhibited good separation and selectivity for the aromatic acids investigated. The effects of eluant pH, eluant ion concentration and solvent composites on the separations were investigated. Organic acids with different substituents were eluted in order of decreasing dissociation coefficients, with no observable peak shape differences.
authors.affiliation: College of Chemistry and Environment, South China Normal University, Guangzhou 510006, China. xia_oxiang@126.com
authors.forename: Juxiang
authors.initials: J
authors.lastname: Ruan
authors.forename: Xiongjun
authors.initials: X
authors.lastname: Zuo
authors.forename: Qiwei
authors.initials: Q
authors.lastname: Lang
authors.forename: Zhijian
authors.initials: Z
authors.lastname: Zeng
authors.forename: Chao
authors.initials: C
authors.lastname: Li
mesh_terms: D000838:Anions / Q000737:chemistry; D001372:Aza Compounds / Q000737:chemistry*; D001555:Benzene Derivatives / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D002852:Chromatography, Ion Exchange / Q000295:instrumentation* / Q000379:methods*; D003510:Cyclohexanes / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007536:Isomerism; D000432:Methanol / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D000838:Anions; D001372:Aza Compounds; D001555:Benzene Derivatives; D003510:Cyclohexanes; D012822:Silicon Dioxide; D000432:Methanol