title: Migration of human dental follicle cells in vitro.
abstract: OBJECTIVE
  The objective of this study was to elucidate the effects of different growth factors on the migration of dental follicle cells (DFCs). DFCs are ectomesenchymally derived easily accessible multipotent stem cells. Cell migration is a crucial step in many biological processes but also for tissue engineering. Growth factors such as epidermal growth factor (EGF), bone morphogenetic protein-2 (BMP2) or transforming growth factor β1 (TGF-β1) can be used to modify the behavior of cells.
  
  
  METHODS
  We used different migration assays (gel spot assay, scratch assay, transwell assay) to evaluate the influence of EGF, BMP2 and TGF-β1 on the migration of DFCs. We investigated the expression of migration-related genes after growth factor stimulation using the PCR array human cell motility.
  
  
  RESULTS
  DFCs treated with BMP2 or TGF-β1 migrated faster than DFCs treated with EGF. Additionally, more migration-related genes are regulated after treatment with BMP2 or TGF-β1 than with EGF. TGF-β1 additionally functions as a chemoattractant for DFCs. Osteogenic differentiation markers were regulated after BMP2 treatment only.
  
  
  CONCLUSIONS
  Whereas the strong migration induced by BMP2 was accompanied by beginning osteogenic differentiation the strong migration induced by TGF-β1 was directional. EGF exhibited not only the weakest migration stimulation but also the weakest induction of differentiation into mineralizing cells.
authors.affiliation: Department of Cranio- and Maxillofacial Surgery, University of Regensburg, Regensburg, Germany.
authors.forename: O
authors.initials: O
authors.lastname: Felthaus
authors.forename: M
authors.initials: M
authors.lastname: Gosau
authors.forename: T
authors.initials: T
authors.lastname: Ettl
authors.forename: L
authors.initials: L
authors.lastname: Prantl
authors.forename: C
authors.initials: C
authors.lastname: Morsczeck
mesh_terms: D015415:Biomarkers / Q000032:analysis; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects / Q000235:genetics; D002478:Cells, Cultured; D002630:Chemotactic Factors / Q000494:pharmacology; D003795:Dental Sac / Q000166:cytology* / Q000187:drug effects; D004815:Epidermal Growth Factor / Q000494:pharmacology; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D006801:Humans; D039902:Multipotent Stem Cells / Q000166:cytology / Q000187:drug effects; D010012:Osteogenesis / Q000187:drug effects; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology
chemical_list: C525718:BMP2 protein, human; D015415:Biomarkers; D055396:Bone Morphogenetic Protein 2; D002630:Chemotactic Factors; D053773:Transforming Growth Factor beta1; D004815:Epidermal Growth Factor
keywords: bone morphogenetic protein-2; cell migration; dental follicle cells; epidermal growth factor; transforming growth factor β1