title: Interaction of sol-gel derived tiO2- and siO2-based bionanocomposites with erythrocytes and serum proteins.
abstract: Nanoenabled drug carriers are emerging as alternatives to conventional small-molecule drugs and their in-vitro biocompatibility evaluation with blood components is a necessary part of early preclinical development. In the present study, Chitosan-based bionanocomposites of sol-gel derived TiO2 and SiO2 were synthesised by the ex situ process. Samples were characterised by X-ray diffraction, Fourier transformed infrared spectrophotometer, transmission electron microscopy and selected area electron diffraction techniques. The analysis reveals the formation of single-phase oxides and their bionanocomposites. Haemolysis (destruction of red blood cell) study was performed by spectrophotometer to assess the haemocompatibility nature as a function of different incubation time. Experimental results reveal the percentage of haemolysis increase with the increase of the incubation time, but it was found to be <2%. This confirms our bionanocomposites are more haemocompatible as compared with respective nanocrystalline ceramics. Also, the interaction of our bionanocomposites with serum proteins at various incubation periods (1-24 h) were investigated using sodium dodecyl sulphate-polyacrylamide gel experiment, the obtained results were discussed in details.
authors.affiliation: Department of Medical Bionanotechnology, Chettinad Hospital and Research Institute, Kelambakkam, Tamil Nadu 603103, India.
authors.forename: A Shakila
authors.initials: AS
authors.lastname: Banu
authors.forename: S Vimala
authors.initials: SV
authors.lastname: Devi
authors.forename: T
authors.initials: T
authors.lastname: Prakash
mesh_terms: D001798:Blood Proteins / Q000737:chemistry* / Q000378:metabolism; D048271:Chitosan / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000737:chemistry* / Q000378:metabolism; D006461:Hemolysis / Q000187:drug effects; D006801:Humans; D046529:Microscopy, Electron, Transmission; D053761:Nanocomposites / Q000737:chemistry* / Q000648:ultrastructure; D044367:Phase Transition; D012822:Silicon Dioxide / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D014025:Titanium / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D014961:X-Ray Diffraction
chemical_list: D001798:Blood Proteins; C009495:titanium dioxide; D012822:Silicon Dioxide; D048271:Chitosan; D014025:Titanium