title: Purification of RNA from milk whey.
abstract: MicroRNAs (miRNAs) are small regulatory RNA molecules that modulate specific target mRNAs and play very important roles in physiological processes. They were recently detected in body fluids such as blood, urine, saliva, and milk. These body fluid miRNAs have been studied thoroughly as potential diagnostic biomarkers. However, there have been few studies of milk miRNAs, and their roles are not clearly understood. Milk is the only nutritional source for newborn infants, and bovine milk is used widely as a dairy product. Thus, it is important to study milk miRNAs. In general, body fluid RNA concentrations are extremely low and of diverse existence types. In this chapter, we compare two silica membrane column-based RNA purification kits, and also compare RNA obtained directly from whey with that isolated from whey-derived exosomes.
authors.affiliation: Nutritional Science Institute, Morinaga Milk Industry Co. Ltd., Kanagawa, Japan.
authors.forename: Hirohisa
authors.initials: H
authors.lastname: Izumi
authors.forename: Nobuyoshi
authors.initials: N
authors.lastname: Kosaka
authors.forename: Takashi
authors.initials: T
authors.lastname: Shimizu
authors.forename: Kazunori
authors.initials: K
authors.lastname: Sekine
authors.forename: Takahiro
authors.initials: T
authors.lastname: Ochiya
authors.forename: Mitsunori
authors.initials: M
authors.lastname: Takase
mesh_terms: D000818:Animals; D002417:Cattle; D005591:Chemical Fractionation / Q000379:methods*; D002850:Chromatography, Gel; D055354:Exosomes / Q000737:chemistry*; D006041:Goats; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D035683:MicroRNAs / Q000302:isolation & purification*; D008892:Milk / Q000737:chemistry*; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012822:Silicon Dioxide
chemical_list: D035683:MicroRNAs; D012822:Silicon Dioxide