title: Zebrafish CD59 has both bacterial-binding and inhibiting activities.
abstract: CD59, known as protectin, usually plays roles as a regulatory inhibitor of complement, but it also exhibits activities independent of its function as a complement inhibitor. This study reported the identification and characterization of an ortholog of mammalian cd59 from zebrafish Danio rerio, which is similar to known cd59 in terms of both amino acid sequence and genomic structure as well as synteny conservation. We showed that zebrafish cd59 was maternally expressed in early embryos and expressed in a tissue-specific manner, with most abundant expression in the brain. We further showed that recombinant zebrafish CD59 was capable of binding to both the Gram-negative and Gram-positive bacteria as well as the microbial signature molecules LPS and LTA. In addition we demonstrated that recombinant zebrafish CD59 displayed slight antimicrobial activity capable of inhibiting the growth of E. coli and S. aureus. All these data indicate that zebrafish CD59 can not only binds to the bacteria and their signature molecules LPS and LTA but can also inhibit their growth, a novel role assigned to CD59.
authors.affiliation: Laboratory for Evolution & Development, Institute of Evolution & Marine Biodiversity and Department of Marine Biology, Ocean University of China, Qingdao 266003, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Sun
authors.forename: Jie
authors.initials: J
authors.lastname: Wu
authors.forename: Shousheng
authors.initials: S
authors.lastname: Liu
authors.forename: Hongyan
authors.initials: H
authors.lastname: Li
authors.forename: Shicui
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001419:Bacteria / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D001483:Base Sequence; D015153:Blotting, Western; D001921:Brain / Q000196:embryology / Q000276:immunology / Q000378:metabolism; D018957:CD59 Antigens / Q000145:classification / Q000235:genetics / Q000276:immunology*; D004926:Escherichia coli / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D057927:Hydrophobic and Hydrophilic Interactions; D008070:Lipopolysaccharides / Q000276:immunology / Q000378:metabolism; D008297:Male; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D011485:Protein Binding; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013211:Staphylococcus aureus / Q000187:drug effects / Q000276:immunology / Q000378:metabolism; D015027:Zebrafish / Q000196:embryology / Q000235:genetics / Q000276:immunology*; D029961:Zebrafish Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology*
chemical_list: D018957:CD59 Antigens; D008070:Lipopolysaccharides; D011994:Recombinant Proteins; D029961:Zebrafish Proteins
keywords: Antimicrobial activity; Bacterial-binding activity; CD59; Complement; Zebrafish