title: Analysis of glabrous canary seeds by ELISA, mass spectrometry, and Western blotting for the absence of cross-reactivity with major plant food allergens.
abstract: Glabrous (hairless) canary seed belongs to the Poaceae (Gramineae) family and could serve as an alternative source of gluten-free cereal grain. In this study, allergenic cross-reactivities between hairless, dehulled canary seeds (Phalaris canariensis) and major allergenic proteins from gluten, soy, peanuts, tree nuts, sesame, and mustard were studied using commercial enzyme-linked immune sorbent assay (ELISA) kits specific for these target allergens. Mass spectrometry (MS) and immunoblotting were further used to assess for the presence of gluten-specific protein fragments. MS results revealed the likely presence of proteins homologous with rice, oat, corn, carrot, tomato, radish, beet, and chickpea. However, no presence of celiac-related gluten fragments from wheat, rye, barley, or their derivatives was found. Immunoblotting studies yielded negative results, further confirming the absence of gluten in the canary seed samples tested. No cross-reactivities were detected between canary seeds and almond, hazelnut, mustard, peanut, sesame, soy, walnut, and gluten using ELISA.
authors.affiliation: Food Research and Development Centre, Agriculture and Agri-Food Canada , 3600 Casavant Boulevard West, Saint-Hyacinthe, Quebec, Canada.
authors.forename: Joyce Irene
authors.initials: JI
authors.lastname: Boye
authors.forename: Allaoua
authors.initials: A
authors.lastname: Achouri
authors.forename: Nancy
authors.initials: N
authors.lastname: Raymond
authors.forename: Chantal
authors.initials: C
authors.lastname: Cleroux
authors.forename: Dorcas
authors.initials: D
authors.lastname: Weber
authors.forename: Terence B
authors.initials: TB
authors.lastname: Koerner
authors.forename: Pierre
authors.initials: P
authors.lastname: Hucl
authors.forename: Carol Ann
authors.initials: CA
authors.lastname: Patterson
mesh_terms: D052179:Antigens, Plant / Q000737:chemistry* / Q000276:immunology; D010367:Arachis / Q000737:chemistry / Q000276:immunology; D015153:Blotting, Western; D031211:Corylus / Q000737:chemistry / Q000276:immunology; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D005983:Glutens / Q000737:chemistry* / Q000276:immunology; D013058:Mass Spectrometry; D031783:Phalaris / Q000737:chemistry* / Q000276:immunology; D027861:Prunus / Q000737:chemistry / Q000276:immunology; D012639:Seeds / Q000737:chemistry* / Q000276:immunology; D031685:Sesamum / Q000737:chemistry / Q000276:immunology
chemical_list: D052179:Antigens, Plant; D005983:Glutens