title: Development and validation of HPLC coupled with triple quadrupole MS for the simultaneous determination of six phenolic acids, six flavonoids, and a lignan in Polygonum capitatum.
abstract: Polygonum capitatum, a traditional Miao medicinal plant, has significant effects on the treatment of urinary system infections and pyelonephritis. However, no study about the comprehensive quality evaluation of P. capitatum has been reported. In this contribution, a rapid and validated method based on HPLC coupled with triple quadrupole MS was established for the simultaneous determination of six active flavonoids, six phenolic acids, and a lignan in extracts of P. capitatum. These compounds were separated within 10 min on a C18 analytical column with gradient elution. All analyses were performed on an Agilent XDB C18 column (2.1 × 100 mm, 3.5 μm) with a linear gradient elution of acetonitrile/water. The proposed method was applied to analyze 15 batches of samples with acceptable linearity (r(2) , 0.9923-0.9992), precisions (RSD, 1.0-3.0%), repeatability (RSD, 2.0-3.2%), stability (RSD, 2.2-3.2%), and recovery (RSD, 2.1-3.6%) of the 13 compounds. These results demonstrated that this presented method was effective and reliable for the comprehensive quality evaluation of P. capitatum. Moreover, our study can provide chemical evidence to reveal the material basis of its therapeutic effects.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Kaixia
authors.initials: K
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Shenghua
authors.initials: S
authors.lastname: Wei
authors.forename: Wenguang
authors.initials: W
authors.lastname: Jing
authors.forename: Yuesheng
authors.initials: Y
authors.lastname: Wang
authors.forename: An
authors.initials: A
authors.lastname: Liu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis*; D062385:Hydroxybenzoates / Q000032:analysis*; D017705:Lignans / Q000032:analysis*; D013058:Mass Spectrometry; D028502:Polygonum / Q000737:chemistry*
chemical_list: D005419:Flavonoids; D062385:Hydroxybenzoates; D017705:Lignans; C017616:phenolic acid
keywords: Flavonoids; HPLC coupled with triple quadrupole mass spectrometry; Phenolic acids; Polygonum capitatum; Schizandriside