title: Structure of diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase from Acinetobacter baumannii.
abstract: The bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase (RibD) represents a potential antibacterial drug target. The structure of recombinant Acinetobacter baumannii RibD is reported in orthorhombic and tetragonal crystal forms at 2.2 and 2.0 Å resolution, respectively. Comparisons with orthologous structures in the Protein Data Bank indicated close similarities. The tetragonal crystal form was obtained in the presence of guanosine monophosphate, which surprisingly was observed to occupy the adenine-binding site of the reductase domain.
authors.affiliation: Division of Biological Chemistry and Drug Discovery, College of Life Sciences, University of Dundee, Dundee DD1 5EH, Scotland.
authors.forename: Alice
authors.initials: A
authors.lastname: Dawson
authors.forename: Paul
authors.initials: P
authors.lastname: Trumper
authors.forename: Georgios
authors.initials: G
authors.lastname: Chrysostomou
authors.forename: William N
authors.initials: WN
authors.lastname: Hunter
mesh_terms: D040981:Acinetobacter baumannii / Q000201:enzymology* / Q000235:genetics; D000595:Amino Acid Sequence; D018360:Crystallography, X-Ray; D009709:Nucleotide Deaminases / Q000737:chemistry* / Q000235:genetics; D010088:Oxidoreductases / Q000737:chemistry* / Q000235:genetics; D017433:Protein Structure, Secondary; D014498:Uracil / Q000737:chemistry*
chemical_list: D014498:Uracil; D010088:Oxidoreductases; D009709:Nucleotide Deaminases
keywords: Acinetobacter baumannii; RibD; bifunctional deaminase/reductase; riboflavin biosynthesis