title: Expressional time phase of leukocyte molecules induced by allogenic cardiac antigen and cyclosporin A in rats' in vitro model.
abstract: The immunosuppressive agent cyclosporin A has been proven to reduce the rejection rate and prolong the survival time of transplanted hearts. But some reports showed that cyclosporine A did not completely suppress the rejection. We performed in vitro studies to model a time course to observe the effect of cyclosporin A.
  
  
  METHODS
  The experiment was divided into a control group (group I), an antigen group (group II), a cyclosporin A group (group III) and an antigen + cyclosporin A group (group IV). After transplantation, at 2 h, 6 h, 12 h, 24 h, 48 h and 72 h, leukocyte molecules were monitored.
  
  
  RESULTS
  The expression of IL-2R peaked at 12 h in group II and at 6 h in group III. There was a gradual decline in the expression of the P59 gene in group I, positive expression at 2 h and between 12 h and 24 h in group II, in group IV, there was a decrease at 48 h. The expression of the CD4 gene was lowest at 2 h in group I and at 6 h in group II. CD4 expression then quickly increased to a maximum at 48 h in group III, at 2 h in group IV. There was a minimal expression was reached at 12 h in group I and IV and at 6 h in group III in the expression of the CD8 gene.
  
  
  CONCLUSIONS
  Alloantigen induced lymphocytes to release IL-2R and P59 and stimulated the induction of the CD4 gene' transcription for 6 h. Cyclosporin A stimulated the release of IL-2R for 2 h. These results provide an in vitro basis for describing the time phases of rejection inhibited by cyclosporin A.
authors.affiliation: Department of Molecular Biology, Capital Medical University Affiliated with Beijing Anzhen Hospital, Beijing Institute of Heart, Lung & Blood Vessel Diseases Beijing 100029, China.
authors.forename: Xiu-Fang
authors.initials: XF
authors.lastname: Xu
authors.forename: Yi
authors.initials: Y
authors.lastname: Xin
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Yi-Min
authors.initials: YM
authors.lastname: Huang
authors.forename: Yun
authors.initials: Y
authors.lastname: Gu
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.forename: Wen-Bin
authors.initials: WB
authors.lastname: Li
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Zhou
authors.forename: Zhao-Guang
authors.initials: ZG
authors.lastname: Zhang
keywords: Transplantation rejection model in vitro; cyclosporin A; early expression’ time phase