title: Molecular cloning of pepsinogens A and C from adult newt (Cynops pyrrhogaster) stomach.
abstract: The full-length cDNAs of three pepsinogens (Pgs) were cloned from the stomach of newt, Cynops pyrrhogaster, and nucleotide sequences of the full-length cDNAs were determined. Molecular phylogenetic analysis showed that two Pgs, named PgC1 and PgC2, belong to the pepsinogen C group, and one Pg, named PgA, belongs to the pepsinogen A group. The sequences contain an open reading frame (ORF) encoding 385 amino acid residues for PgC1, 383 amino acid residues for PgC2 and 377 amino acid residues for PgA. In addition, all of the three amino acid sequences conserve some unique characteristics such as six cysteine residues and putative active site two aspartic acid residues. All of the pepsinogen mRNAs were detected in the stomach by RT-PCR but not in other organs. Although a slight difference at the time of the start of expression was seen among the three pepsinogen genes, all of them were expressed in the larval stage after hatching. This is the first report on cloning of pepsinogens from urodele stomach.
authors.affiliation: Department of Biology, Faculty of Education, Utsunomiya University, 350 Mine, Utsunomiya, Tochigi, 321-8505, Japan. inokuchi@cc.utsunomiya-u.ac.jp
authors.forename: Tomofumi
authors.initials: T
authors.lastname: Inokuchi
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ikuzawa
authors.forename: Shin
authors.initials: S
authors.lastname: Yamazaki
authors.forename: Yukari
authors.initials: Y
authors.lastname: Watanabe
authors.forename: Koushiro
authors.initials: K
authors.lastname: Shiota
authors.forename: Takuma
authors.initials: T
authors.lastname: Katoh
authors.forename: Ken-Ichiro
authors.initials: K
authors.lastname: Kobayashi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005753:Gastric Mucosa / Q000378:metabolism*; D016013:Likelihood Functions; D008957:Models, Genetic; D008969:Molecular Sequence Data; D020116:Pepsinogen A / Q000235:genetics* / Q000378:metabolism; D020115:Pepsinogen C / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012455:Salamandridae / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D017931:DNA Primers; D018076:DNA, Complementary; D020115:Pepsinogen C; D020116:Pepsinogen A