title: 7-((5-Nitrothiophen-2-yl)methoxy)-3H-phenoxazin-3-one as a spectroscopic off-on probe for highly sensitive and selective detection of nitroreductase.
abstract: A new spectroscopic off-on probe, 7-((5-nitrothiophen-2-yl)methoxy)-3H-phenoxazin-3-one, is developed and applied to real-time detection of nitroreductase produced by Escherichia coli.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Li
authors.forename: Xinghui
authors.initials: X
authors.lastname: Gao
authors.forename: Wen
authors.initials: W
authors.lastname: Shi
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Li
authors.forename: Huimin
authors.initials: H
authors.lastname: Ma
mesh_terms: D004926:Escherichia coli / Q000201:enzymology; D029968:Escherichia coli Proteins / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D015335:Molecular Probes / Q000737:chemistry*; D009601:Nitroreductases / Q000032:analysis*; D010078:Oxazines / Q000138:chemical synthesis / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013696:Temperature
chemical_list: C585348:7-((5-nitrothiophen-2-yl)methoxy)-3H-phenoxazin-3-one; D029968:Escherichia coli Proteins; D015335:Molecular Probes; D010078:Oxazines; C020456:phenoxazinone; C116053:NfsA protein, E coli; D009601:Nitroreductases