title: Relationship between membrane damage, leakage of intracellular compounds, and inactivation of Escherichia coli treated by pressurized CO2.
abstract: The relationship between membrane damage, leakage of intracellular compounds, and inactivation of Escherichia coli treated by pressurized CO2 was investigated by assessing the inactivation, bacterial cell membrane permeability, the leakage of protein, nucleic acid, and K(+) and Mg(2+) of E. coli. The results indicated that pressurized CO2 treatment induced the leakage of protein in E. coli, but the time of leakage was lagged behind the time of 99% E. coli inactivation, so it was only the secondary phenomenon of inactivation. The inactivation of E. coli was related to the leakage of nucleic acid, K(+) , Mg(2+) induced by the pressurized CO2 treatment. There was direct relationship between the inactivation of E. coli and the damaging effect of pressurized CO2 treatment on the cell membrane of E. coli.
authors.affiliation: Key Laboratory of Dairy Science of Ministry of Education, Northeast Agricultural University, Harbin, China; Food College, Northeast Agricultural University, Harbin, China; Food College, Heilongjiang Polytechnic, Harbin, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Yao
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Li
authors.forename: Weiwei
authors.initials: W
authors.lastname: Bi
authors.forename: Chen
authors.initials: C
authors.lastname: Jiang
mesh_terms: D002245:Carbon Dioxide / Q000494:pharmacology*; D002462:Cell Membrane / Q000473:pathology*; D002463:Cell Membrane Permeability / Q000502:physiology*; D004203:Disinfection / Q000379:methods*; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development*; D005519:Food Preservation; D059022:Food Safety; D008274:Magnesium; D050296:Microbial Viability; D011188:Potassium
chemical_list: D002245:Carbon Dioxide; D008274:Magnesium; D011188:Potassium
keywords: Cell membrane permeability; K+ and Mg2+ leakage; Nucleic acid leakage; Pressurized CO2; Protein leakage