title: Species selective diazirine positioning in tag-free photoactive quorum sensing probes.
abstract: The synthesis and comparison of activities of 'tag-free' probes with diazirines at various positions are described. Remarkable differences in their effects on P. aeruginosa and on human bronchial epithelial cells were observed, supporting the efforts to isolate and identify receptors for N-acyl homoserine lactones.
authors.affiliation: Department of Chemistry and the National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Be'er-Sheva 84105, Israel.
authors.forename: Luba
authors.initials: L
authors.lastname: Dubinsky
authors.forename: Antonia
authors.initials: A
authors.lastname: Delago
authors.forename: Neri
authors.initials: N
authors.lastname: Amara
authors.forename: Pnina
authors.initials: P
authors.lastname: Krief
authors.forename: Josep
authors.initials: J
authors.lastname: Rayo
authors.forename: Tsaffrir
authors.initials: T
authors.lastname: Zor
authors.forename: Vladimir V
authors.initials: VV
authors.lastname: Kravchenko
authors.forename: Michael M
authors.initials: MM
authors.lastname: Meijler
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D061565:Cycloaddition Reaction; D003978:Diazomethane / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D015335:Molecular Probes / Q000737:chemistry / Q000494:pharmacology; D017434:Protein Structure, Tertiary; D011550:Pseudomonas aeruginosa / Q000378:metabolism; D053038:Quorum Sensing / Q000187:drug effects*; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D001426:Bacterial Proteins; C080365:LasR protein, Pseudomonas aeruginosa; D015335:Molecular Probes; D015534:Trans-Activators; D003978:Diazomethane; D002265:Carboxylic Ester Hydrolases; C432003:N-acyl homoserine lactonase