title: Reactive oxygen species promote chloroplast dysfunction and salicylic acid accumulation in fumonisin B1-induced cell death.
abstract: We report a novel regulatory mechanism by which reactive oxygen species (ROS) regulate fumonisin B1 (FB1)-induced cell death. We found that FB1 induction of light-dependent ROS production promoted the degradation of GFP-labeled chloroplast proteins and increased phenylalanine ammonia lyase (PAL) activity, PAL1 gene expression and SA content, while pretreatment with ROS manipulators reversed these trends. Moreover, treatment with H2O2 or 3-amino-1,2,4-triazole increased PAL activity, PAL1 gene expression and SA content. PAL inhibitor significantly blocked FB1-induced lesion formation and SA increase. Our results demonstrate that light-dependent ROS accumulation stimulates the degradation of chloroplastic proteins and up-regulates PAL-mediated SA synthesis, thus promoting FB1-induced light-dependent cell death.
authors.affiliation: MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
authors.forename: Fuqiang
authors.initials: F
authors.lastname: Xing
authors.forename: Zhe
authors.initials: Z
authors.lastname: Li
authors.forename: Aizhen
authors.initials: A
authors.lastname: Sun
authors.forename: Da
authors.initials: D
authors.lastname: Xing
mesh_terms: D000640:Amitrole / Q000494:pharmacology; D017360:Arabidopsis / Q000166:cytology / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D029681:Arabidopsis Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects*; D002736:Chloroplasts / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D037341:Fumonisins / Q000494:pharmacology*; D015870:Gene Expression; D006861:Hydrogen Peroxide / Q000494:pharmacology; D016877:Oxidants / Q000494:pharmacology; D010650:Phenylalanine Ammonia-Lyase / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D018515:Plant Leaves / Q000166:cytology / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D059748:Proteolysis; D017382:Reactive Oxygen Species / Q000378:metabolism*; D020156:Salicylic Acid / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D029681:Arabidopsis Proteins; D037341:Fumonisins; D016877:Oxidants; D017382:Reactive Oxygen Species; C056933:fumonisin B1; D006861:Hydrogen Peroxide; D010650:Phenylalanine Ammonia-Lyase; D020156:Salicylic Acid; D000640:Amitrole