title: Cytotoxicity and inflammatory potential of two Pseudomonas mosselii strains isolated from clinical samples of hospitalized patients.
abstract: BACKGROUND
  The genus Pseudomonas includes a heterogeneous set of microorganisms that can be isolated from many different niches and nearly 100 different strains have been described. The best characterized bacterium is Pseudomonas aeruginosa which is the primary agent of opportunistic infection in humans, causing both acute and chronic infections. Other species like fluorescens, putida or mosselii have been sporadically isolated from hospitalized patients but their association with the pathology often remains unclear.
  
  
  RESULTS
  This study focuses on the cytotoxicity and inflammatory potential of two strains of Pseudomonas mosselii (ATCC BAA-99 and MFY161) that were recently isolated from clinical samples of hospitalized patients. The behavior of these bacteria was compared to that of the well-known opportunistic pathogen P. aeruginosa PAO1. We found that P. mosselii ATCC BAA-99 and MFY161 are cytotoxic towards Caco-2/TC7 cells, have low invasive capacity, induce secretion of human β-defensin 2 (HBD-2), alter the epithelial permeability of differentiated cells and damage the F-actin cytoskeleton.
  
  
  CONCLUSIONS
  These data bring new insights into P. mosselii virulence, since this bacterium has often been neglected due to its rare occurrence in hospital.
authors.affiliation: Laboratoire de Microbiologie Signaux et Microenvironnement-LMSM EA 4312, Université de Rouen, Normandie Université, 55 Rue Saint-Germain, Evreux F-27000, France.
authors.forename: Charlène
authors.initials: C
authors.lastname: Leneveu-Jenvrin
authors.forename: Amar
authors.initials: A
authors.lastname: Madi
authors.forename: Emeline
authors.initials: E
authors.lastname: Bouffartigues
authors.forename: Kelly
authors.initials: K
authors.lastname: Biaggini
authors.forename: Marc
authors.initials: M
authors.lastname: Feuilloley
authors.forename: Sylvie
authors.initials: S
authors.lastname: Chevalier
authors.forename: Nathalie
authors.initials: N
authors.lastname: Connil
mesh_terms: D000199:Actins / Q000378:metabolism; D001427:Bacterial Toxins / Q000378:metabolism*; D002460:Cell Line; D002463:Cell Membrane Permeability; D004705:Endocytosis; D004847:Epithelial Cells / Q000382:microbiology / Q000502:physiology; D006761:Hospitals; D006801:Humans; D011549:Pseudomonas / Q000302:isolation & purification / Q000472:pathogenicity*; D011552:Pseudomonas Infections / Q000382:microbiology*; D014774:Virulence; D023083:beta-Defensins / Q000378:metabolism
chemical_list: D000199:Actins; D001427:Bacterial Toxins; C106641:DEFB4A protein, human; D023083:beta-Defensins