title: Wickerhamiella slavikovae sp. nov. and Wickerhamiella goesii sp. nov., two yeast species isolated from natural substrates.
abstract: Two novel yeast species were isolated during three independent studies of yeasts associated with natural substrates in Brazil and Taiwan. Analysis of the sequences of the D1/D2 domains of the large subunit rRNA gene showed that these novel species belong to the Wickerhamiella clade. The first was isolated from freshwater and a leaf of sugar cane (Saccharum officinarum) in Brazil and from leaves of Wedelia biflora in Taiwan. Described here as Wickerhamiella slavikovae sp. nov., it differs by 56 nucleotide substitutions and 19 gaps in the D1/D2 region of the large subunit rRNA gene from Candida sorbophila, the least divergent species. The second species, named Wickerhamiella goesii sp. nov., was isolated from leaves and the rhizosphere of sugar cane collected in Rio de Janeiro, Brazil. The species differs by 54 nucleotide substitutions and nine gaps in the D1/D2 domains from Candida drosophilae, its least divergent relative. The type strains are Wickerhamiella slavikovae sp. nov. IMUFRJ 52096(T) (= CBS 12417(T) = DBVPG 8032(T)) and Wickerhamiella goesii sp. nov. IMUFRJ 52102(T) (= CBS 12419(T) = DBVPG 8034(T)).
authors.affiliation: Departamento de Microbiologia, Instituto de Microbiologia Prof. Paulo de Goes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Allen N
authors.initials: AN
authors.lastname: Hagler
authors.affiliation: Departamento de Microbiologia, Instituto de Microbiologia Prof. Paulo de Goes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: José R A
authors.initials: JRA
authors.lastname: Ribeiro
authors.affiliation: Departamento de Microbiologia, Instituto de Microbiologia Prof. Paulo de Goes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: T
authors.initials: T
authors.lastname: Pinotti
authors.affiliation: Departamento de Microbiologia, ICB, C. P. 486, Universidade Federal de Minas Gerais, Belo Horizonte, MG 3120-901, Brazil.
authors.forename: Luciana R
authors.initials: LR
authors.lastname: Brandão
authors.affiliation: Laboratório de Microbiologia Ambiental e Biotecnologia, Campus Universitário de Palmas, Universidade Federal do Tocantins, 77020220 Palmas, TO, Brazil.
authors.forename: Raphael S
authors.initials: RS
authors.lastname: Pimenta
authors.affiliation: Departamento de Microbiologia, Instituto de Microbiologia Prof. Paulo de Goes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: U
authors.initials: U
authors.lastname: Lins
authors.affiliation: Department of Applied Science, National Hsinchu University of Education, 521 Nanda Road, Hsinchu 30014, Taiwan, ROC.
authors.forename: Ching-Fu
authors.initials: CF
authors.lastname: Lee
authors.affiliation: Department of Applied Science, National Hsinchu University of Education, 521 Nanda Road, Hsinchu 30014, Taiwan, ROC.
authors.forename: Chin-Wen
authors.initials: CW
authors.lastname: Hsieh
authors.affiliation: Department of Biology, University of Western Ontario, London, Ontario N6A 5B7, Canada.
authors.forename: Marc-André
authors.initials: MA
authors.lastname: Lachance
authors.affiliation: Departamento de Microbiologia, ICB, C. P. 486, Universidade Federal de Minas Gerais, Belo Horizonte, MG 3120-901, Brazil.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Rosa
mesh_terms: D001938:Brazil; D004271:DNA, Fungal / Q000235:genetics; D005618:Fresh Water / Q000382:microbiology; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010802:Phylogeny; D018515:Plant Leaves / Q000382:microbiology; D054683:Ribosome Subunits, Large, Eukaryotic / Q000235:genetics; D004718:Saccharomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D031786:Saccharum / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013624:Taiwan; D031209:Wedelia / Q000382:microbiology
chemical_list: D004271:DNA, Fungal