title: Expression profile analysis of antioxidative stress and developmental pathway genes in the manganese-exposed intertidal copepod Tigriopus japonicus with 6K oligochip.
abstract: Manganese (Mn) provides one of aquatic pollutants in marine ecosystem. Here we used a 6K oligomicroarray to identify the effect of Mn on transcriptomes in the copepod Tigriopus japonicus. A total of 5594 spots were significantly modulated on a 6K oligomicroarray with hierarchical clustering after exposure to Mn over 24h. Of them, 186 and 489 genes were significantly upregulated and downregulated, respectively. Particularly, several genes involved in stress, detoxification, and developmental functions were significantly modulated in T. japonicus exposed for 24h. In detail, Mn exposure specifically up-regulated genes that were related to intracellular stress, antioxidant, and detoxification pathways such as cytochrome P450s (CYPs), glutathione S-transferases (GSTs), and heat shock proteins (hsps), while a majority of downregulated genes was associated with developmental pathways such as cuticle protein, ecdysone receptor, and vitellogenin. These results demonstrated that Mn exposure modulated gene expression in relation to intracellular stress, leading to developmental retardation in the intertidal copepod, T. japonicus, and provide a better understanding of mechanistic molecular studies of Mn-induced cellular damage.
authors.affiliation: Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul 133-791, South Korea.
authors.forename: Bo-Mi
authors.initials: BM
authors.lastname: Kim
authors.forename: Beom-Soon
authors.initials: BS
authors.lastname: Choi
authors.forename: Kyun-Woo
authors.initials: KW
authors.lastname: Lee
authors.forename: Jang-Seu
authors.initials: JS
authors.lastname: Ki
authors.forename: Il-Chan
authors.initials: IC
authors.lastname: Kim
authors.forename: Ik-Young
authors.initials: IY
authors.lastname: Choi
authors.forename: Jae-Sung
authors.initials: JS
authors.lastname: Rhee
authors.forename: Jae-Seong
authors.initials: JS
authors.lastname: Lee
mesh_terms: D000818:Animals; D016000:Cluster Analysis; D033342:Copepoda / Q000187:drug effects* / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D008345:Manganese / Q000737:chemistry / Q000633:toxicity*; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000378:metabolism; D011987:Receptors, Steroid / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects; D014819:Vitellogenins / Q000235:genetics / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000633:toxicity*
chemical_list: D006360:Heat-Shock Proteins; D012333:RNA, Messenger; D011987:Receptors, Steroid; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; C055469:ecdysone receptor; D008345:Manganese; D003577:Cytochrome P-450 Enzyme System; D005982:Glutathione Transferase
keywords: Gene expression; Microarray; Mn; Real-time RT-PCR; Tigriopus japonicus