title: Purification, crystallization and preliminary X-ray crystallographic studies of the Mycobacterium tuberculosis DNA gyrase ATPase domain.
abstract: Mycobacterium tuberculosis DNA gyrase, a nanomachine involved in the regulation of DNA topology, is the only type II topoisomerase present in this organism and hence is the sole target of fluoroquinolones in the treatment of tuberculosis. The ATPase domain provides the energy required for catalysis by ATP hydrolysis. Two constructs corresponding to this 43 kDa domain, Mtb-GyrB47(C1) and Mtb-GyrB47(C2), have been overproduced, purified and crystallized. Diffraction data were collected from three crystal forms. The crystals belonged to space groups P1 and P21 and diffracted to resolutions of 2.9 and 3.3 Å, respectively.
authors.affiliation: Unité de Microbiologie Structurale, Institut Pasteur, CNRS UMR 3528, 25 rue du Dr Roux, 75015 Paris, France.
authors.forename: Mélanie
authors.initials: M
authors.lastname: Roué
authors.forename: Alka
authors.initials: A
authors.lastname: Agrawal
authors.forename: Craig
authors.initials: C
authors.lastname: Volker
authors.forename: Danuta
authors.initials: D
authors.lastname: Mossakowska
authors.forename: Claudine
authors.initials: C
authors.lastname: Mayer
authors.forename: Benjamin D
authors.initials: BD
authors.lastname: Bax
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry* / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D027081:DNA Gyrase / Q000737:chemistry* / Q000302:isolation & purification; D009169:Mycobacterium tuberculosis / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D000251:Adenosine Triphosphatases; D027081:DNA Gyrase
keywords: ATPase domain; DNA gyrase; Mycobacterium tuberculosis; type II topoisomerase