title: Poly(lactide-co-glycolide) nanoparticles, layer by layer engineered for the sustainable delivery of antiTNF-α.
abstract: A strategy of encapsulation of the antiTNF-α antibody on top of poly(lactide-co-glycolide) nanoparticles (PLGA NPs) is presented on the basis of the complexation of antiTNF-α with alginate (Alg) and subsequent assembly layer by layer with poly(L-lysine) (PLL). The assembly of the antiTNF-α/Alg complex with PLL and its stability in PBS and lysozymes are monitored on a planar support using a quartz crystal microbalance with dissipation. The assembly of the antiTNF-α/Alg complex on PLGA NPs is followed by zeta potential measurements. AntiTNF-α release from the PLGA NPs is measured in PBS at 37 and 60 °C and in the HepG2 cell line following NP uptake, using the Q-ADA kit detection kit. The release follows first-order kinetics with an initial burst. Intracellular release of antiTNF-α is confirmed by confocal Raman microscopy.
authors.affiliation: CIC biomaGUNE, Paseo Miramón 182 C, 20009 San Sebastian, Gipuzkoa, Spain.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Romero
authors.forename: Olaia
authors.initials: O
authors.lastname: Ochoteco
authors.forename: David J
authors.initials: DJ
authors.lastname: Sanz
authors.forename: Irina
authors.initials: I
authors.lastname: Estrela-Lopis
authors.forename: Edwin
authors.initials: E
authors.lastname: Donath
authors.forename: Sergio E
authors.initials: SE
authors.lastname: Moya
mesh_terms: D000464:Alginates / Q000737:chemistry; D000888:Antibodies, Anti-Idiotypic / Q000737:chemistry* / Q000494:pharmacology; D016503:Drug Delivery Systems; D020723:Glucuronic Acid / Q000737:chemistry; D056945:Hep G2 Cells; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D011098:Polyglactin 910 / Q000008:administration & dosage / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000276:immunology*
chemical_list: D000464:Alginates; D000888:Antibodies, Anti-Idiotypic; D006603:Hexuronic Acids; D014409:Tumor Necrosis Factor-alpha; D011098:Polyglactin 910; D020723:Glucuronic Acid
keywords: antiTNF-α; layer by layer assembly; nanoparticles; poly(lactide-co-glycolide); sustainable release