title: Histomorphometric analysis of knee synovial membrane in dogs undergoing leg lengthening by classic Ilizarov method and rapid automatic distraction.
abstract: OBJECTIVE
  This pilot study aimed to test the theory that different lengthening methods affect the microscopic structure of knee joint synovium in diverse ways.
  
  
  METHODS
  This was a descriptive and analytical cross-sectional study of synovium carried out in two experimental models of canine leg lengthening using the Ilizarov fixator. Group 1 (n = 6) used manual gradual distraction most commonly used in clinical practice at one millimetre/day divided into four equal increments, 0.25 mm at each increment. Group 2 (n = 7) used an increased rate of automatic distraction at three millimetres/day divided in 120 increments, 0.025 mm at each increment. At the end of distraction and after fixator removal, the animals were euthanised. Control was via intact dogs. The thickness of the synovial lining layer, number of synovial cell rows, and numerical density of subsynovial microvessels were assessed in digital images for semiautomated computerised analysis of semi-thin sections stained with toluidine blue and methylene blue-basic fuchsin. Comparison of synovitis manifestation was made with grading scale. The vascular and nerve changes in the subsynovial layer were also compared.
  
  
  RESULTS
  Group 1 developed marked synovitis, synovium hypervascularisation, degeneration of the nerve fibres in subsynovial nerves with the tendency to regeneration. Group 2 had moderate to mild degree of synovitis with progressive degenerative changes in subsynovial vessels and nerves.
  
  
  CONCLUSIONS
  Both methods used are unfavourable for the state of the joint synovium, but modify it in different ways.
authors.affiliation: Russian Ilizarov Scientific Center for Restorative Traumatology and Orthopaedics, Kurgan, Russia.
authors.forename: Tatyana A
authors.initials: TA
authors.lastname: Stupina
authors.forename: Mikhail M
authors.initials: MM
authors.lastname: Shchudlo
authors.forename: Natalia A
authors.initials: NA
authors.lastname: Shchudlo
authors.forename: Mikhail A
authors.initials: MA
authors.lastname: Stepanov
mesh_terms: D000818:Animals; D001852:Bone Lengthening / Q000379:methods*; D003430:Cross-Sectional Studies; D004285:Dogs; D018889:Ilizarov Technique; D015994:Incidence; D007719:Knee Joint / Q000473:pathology*; D055806:Microvessels / Q000473:pathology; D023421:Models, Animal; D019857:Osteogenesis, Distraction / Q000379:methods*; D010865:Pilot Projects; D013583:Synovial Membrane / Q000473:pathology*; D013585:Synovitis / Q000453:epidemiology / Q000473:pathology; D013977:Tibia / Q000601:surgery*; D013997:Time Factors