title: Variegated expression of Hsp22 transgenic reporters indicates cell-specific patterns of aging in Drosophila oenocytes.
abstract: The cytoplasmic chaperone gene Hsp70 and the mitochondrial chaperone gene Hsp22 are upregulated during normal aging in Drosophila in tissue-general patterns. In addition, Hsp22 reporters are dramatically upregulated during aging in a subset of the oenocytes (liver-like cells). Hsp22 reporter expression varied dramatically between individual oenocytes and between groups of oenocytes located in adjacent body segments, and was negatively correlated with accumulation of age pigment, indicating cell-specific and cell-lineage-specific patterns of oenocyte aging. Conditional transgenic systems were used to express 88 transgenes to search for trans-regulators of the Hsp70 and Hsp22 reporters during aging. The wingless gene increased tissue-general upregulation of both Hsp70 and Hsp22 reporters. In contrast, the mitochondrial genes MnSOD and Hsp22 increased expression of Hsp22 reporters in the oenocytes and decreased accumulation of age pigment in these cells. The data suggest that cell-specific and cell lineage-specific patterns of mitochondrial malfunction contribute to oenocyte aging.
authors.affiliation: University of Southern California, 1050 Childs Way, RRI 201, Los Angeles, CA 90089-2910. jtower@usc.edu.
authors.forename: John
authors.initials: J
authors.lastname: Tower
authors.forename: Gary
authors.initials: G
authors.lastname: Landis
authors.forename: Rebecca
authors.initials: R
authors.lastname: Gao
authors.forename: Albert
authors.initials: A
authors.lastname: Luan
authors.forename: Jonathan
authors.initials: J
authors.lastname: Lee
authors.forename: Yuanyue
authors.initials: Y
authors.lastname: Sun
mesh_terms: D000375:Aging / Q000235:genetics*; D000818:Animals; D019070:Cell Lineage / Q000235:genetics; D016922:Cellular Senescence / Q000235:genetics; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics*; D005260:Female; D005456:Fluorescent Dyes; D005786:Gene Expression Regulation / Q000235:genetics*; D050259:Genes, Mitochondrial / Q000235:genetics; D017930:Genes, Reporter / Q000235:genetics; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D006360:Heat-Shock Proteins / Q000235:genetics*; D008164:Luminescent Proteins / Q000032:analysis; D008297:Male; D010858:Pigmentation / Q000235:genetics; D013482:Superoxide Dismutase / Q000235:genetics; D019076:Transgenes / Q000235:genetics; D015854:Up-Regulation; D051155:Wnt1 Protein / Q000235:genetics; D000097573:Red Fluorescent Protein; D000099156:Superoxide Dismutase 2
chemical_list: D029721:Drosophila Proteins; D005456:Fluorescent Dyes; D018840:HSP70 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D008164:Luminescent Proteins; D013482:Superoxide Dismutase; D051155:Wnt1 Protein; D000097573:Red Fluorescent Protein; D000099156:Superoxide Dismutase 2; C490752:Hsp22 protein, Drosophila; C074622:wg protein, Drosophila
keywords: Age pigment; Cell lineage; Mitochondria; Mosaic; Prepattern.