title: Inference of the genetic network regulating lateral root initiation in Arabidopsis thaliana.
abstract: Regulation of gene expression is crucial for organism growth, and it is one of the challenges in systems biology to reconstruct the underlying regulatory biological networks from transcriptomic data. The formation of lateral roots in Arabidopsis thaliana is stimulated by a cascade of regulators of which only the interactions of its initial elements have been identified. Using simulated gene expression data with known network topology, we compare the performance of inference algorithms, based on different approaches, for which ready-to-use software is available. We show that their performance improves with the network size and the inclusion of mutants. We then analyze two sets of genes, whose activity is likely to be relevant to lateral root initiation in Arabidopsis, and assess causality of their regulatory interactions by integrating sequence analysis with the intersection of the results of the best performing methods on time series and mutants. The methods applied capture known interactions between genes that are candidate regulators at early stages of development. The network inferred from genes significantly expressed during lateral root formation exhibits distinct scale free, small world and hierarchical properties and the nodes with a high out-degree may warrant further investigation.
authors.affiliation: Centre for Plant Integrative Biology, School of Biosciences, University of Nottingham, Loughborough, United Kingdom. Daniele.Muraro@ndm.ox.ac.uk
authors.forename: Daniele
authors.initials: D
authors.lastname: Muraro
authors.forename: Ute
authors.initials: U
authors.lastname: Voβ
authors.forename: Michael
authors.initials: M
authors.lastname: Wilson
authors.forename: Malcolm
authors.initials: M
authors.lastname: Bennett
authors.forename: Helen
authors.initials: H
authors.lastname: Byrne
authors.forename: Ive
authors.initials: I
authors.lastname: De Smet
authors.forename: Charlie
authors.initials: C
authors.lastname: Hodgman
authors.forename: John
authors.initials: J
authors.lastname: King
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D001499:Bayes Theorem; D019295:Computational Biology; D003198:Computer Simulation; D053263:Gene Regulatory Networks; D018517:Plant Roots / Q000235:genetics* / Q000254:growth & development