title: Effects of chlorhexidine on bonding durability of different adhesive systems using a novel thermocycling method.
abstract: BACKGROUND
  The purpose of this study was to evaluate the influence of chlorhexidine on the bonding durability of etch-and-rinse and self-etch adhesive systems using the polymerase chain reaction (PCR) thermocycling method.
  
  
  METHODS
  Twenty freshly extracted intact human third molars were ground and bonded with either an etch-and-rinse adhesive (Single-Bond) or a self-etch adhesive (G-Bond). Specimens were either left untouched or placed in PCR tubes filled with three thermocycling mediums: water, chlorhexidine or silicone oil. Thermocycling (5000 cycles) was done using the PCR programme at temperatures of 5 °C and 55 °C. The microtensile bond strength (μTBS) was evaluated and interfacial nanoleakage was assessed by scanning electron microscopy before and after thermocycling.
  
  
  RESULTS
  Significant differences were detected among groups kept in different media after thermocycling. For Single-Bond, both the chlorhexidine and silicone oil groups could preserve the μTBS (p < 0.001). For G-Bond, μTBS of the chlorhexidine and water groups were significantly decreased (p < 0.05). No obvious increase in silver deposition was observed in specimens incubated in water after thermocycling, less silver penetration was found in specimens incubated in chlorhexidine.
  
  
  CONCLUSIONS
  In this experimental model, chlorhexidine was found to preserve bonding durability in Single-Bond but have no significant effects on G-Bond.
authors.affiliation: The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST), School and Hospital of Stomatology, Wuhan University, Wuhan, China.
authors.forename: D
authors.initials: D
authors.lastname: Deng
authors.forename: X
authors.initials: X
authors.lastname: Huang
authors.forename: C
authors.initials: C
authors.lastname: Huang
authors.forename: T
authors.initials: T
authors.lastname: Yang
authors.forename: X
authors.initials: X
authors.lastname: Du
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.forename: X
authors.initials: X
authors.lastname: Ouyang
authors.forename: D
authors.initials: D
authors.lastname: Pei
mesh_terms: D000269:Adhesives / Q000737:chemistry*; D017438:Bisphenol A-Glycidyl Methacrylate; D002710:Chlorhexidine / Q000494:pharmacology*; D003080:Cold Temperature; D003188:Composite Resins / Q000737:chemistry; D001840:Dental Bonding / Q000379:methods*; D045662:Dental Etching / Q000379:methods*; D019232:Dental Restoration Failure; D003804:Dentin; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D006358:Hot Temperature; D006801:Humans; D008689:Methacrylates; D008855:Microscopy, Electron, Scanning; D008964:Molar, Third; D016133:Polymerase Chain Reaction / Q000295:instrumentation; D011897:Random Allocation; D019279:Resin Cements; D013997:Time Factors
chemical_list: D000269:Adhesives; D003188:Composite Resins; D017220:Dentin-Bonding Agents; C498353:G-Bond; D008689:Methacrylates; D019279:Resin Cements; C115612:single bond; D017438:Bisphenol A-Glycidyl Methacrylate; D002710:Chlorhexidine