title: Expression profiling of cell cycle genes in human pancreatic islets with and without type 2 diabetes.
abstract: Microarray gene expression data were used to analyze the expression pattern of cyclin, cyclin-dependent kinase (CDKs) and cyclin-dependent kinase inhibitor (CDKIs) genes from human pancreatic islets with and without type 2 diabetes (T2D). Of the cyclin genes, CCNI was the most expressed. Data obtained from microarray and qRT-PCR showed higher expression of CCND1 in diabetic islets. Among the CDKs, CDK4, CDK8 and CDK9 were highly expressed, while CDK1 was expressed at low level. High expression of CDK18 was observed in diabetic islets. Of the CDKIs, CDKN1A expression was higher in diabetic islets in both microarray and qRT-PCR. Expression of CDKN1A, CDKN2A, CCNI2, CDK3 and CDK16 was correlated with age. Finally, eight SNPs in these genes were associated with T2D in the DIAGRAM database. Our data provide a comprehensive expression pattern of cell cycle genes in human islets. More human studies are required to confirm and reproduce animal studies.
authors.affiliation: Lund University Diabetes Center, Department of Clinical Sciences, Diabetes & Endocrinology, Skåne University Hospital, Lund University, Malmö 20502, Sweden. Jalal.Taneera@med.lu.se
authors.forename: Jalal
authors.initials: J
authors.lastname: Taneera
authors.forename: Joao
authors.initials: J
authors.lastname: Fadista
authors.forename: Emma
authors.initials: E
authors.lastname: Ahlqvist
authors.forename: Mengze
authors.initials: M
authors.lastname: Zhang
authors.forename: Nils
authors.initials: N
authors.lastname: Wierup
authors.forename: Erik
authors.initials: E
authors.lastname: Renström
authors.forename: Leif
authors.initials: L
authors.lastname: Groop
mesh_terms: D000368:Aged; D000375:Aging; D016022:Case-Control Studies; D002478:Cells, Cultured; D050756:Cyclin-Dependent Kinase Inhibitor Proteins / Q000235:genetics / Q000378:metabolism; D018844:Cyclin-Dependent Kinases / Q000235:genetics / Q000378:metabolism; D016213:Cyclins / Q000235:genetics / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D018816:Genes, cdc; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide; D059467:Transcriptome
chemical_list: D050756:Cyclin-Dependent Kinase Inhibitor Proteins; D016213:Cyclins; D007328:Insulin; D018844:Cyclin-Dependent Kinases