title: Expression and role of miR-34a in bladder cancer.
abstract: To investigate the biological function of microRNA-34a (miR-34a) in bladder cancer, the expression of miR-34a was determined using quantitative real-time polymerase chain (qRT-PCR) reaction in 42 cases of bladder cancer. The relationship between the expression of miR-34a and development of bladder cancer was also studied. The mature mimics of miR-34a were chemically synthesized and transiently transfected into human bladder cancer T24 cells. The effects of miR-34a on apoptosis, cell cycle and proliferation in T24 cells were evaluated by flow cytometry and MTT, respectively. The results showed that the low expression rate of miR-34a was correlated with the malignancy and tumor size of bladder cancer. The up-regulation of miR-34a in T24 cells contributed to cell growth and cell cycle arrest, but not caspase-3 pathway. These findings suggest that the relative low expression of miRNA-34a might be involved in the tumorigenesis of bladder cancer.
authors.affiliation: Department of Urology, The Affiliated Hospital of Logistics University of the Chinese People's Armed Tianjin, 300162, China. jinglong2000w@163.com
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Tan
authors.initials: T
authors.lastname: Li
authors.forename: Guang
authors.initials: G
authors.lastname: Han
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Shi
authors.forename: Hui
authors.initials: H
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D017209:Apoptosis; D014408:Biomarkers, Tumor; D053148:Caspase 3 / Q000378:metabolism; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000502:physiology*; D008875:Middle Aged; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D013997:Time Factors; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; C522425:MIRN34 microRNA, human; D035683:MicroRNAs; D013778:Tetrazolium Salts; D013844:Thiazoles; D053148:Caspase 3; C022616:thiazolyl blue