title: Analysis of transcriptional profiles to discover biomarker candidates in Mycobacterium avium subsp. paratuberculosis-infected macrophages, RAW 264.7.
abstract: Paratuberculosis (PTB) or Johne's disease is one of the most serious chronic debilitating diseases of ruminants worldwide that is caused by Mycobacterium avium subsp. paratuberculosis (MAP). MAP is a slow-growing bacterium that has very long latent periods, resulting in difficulties in diagnosing and controlling the disease, especially regarding the diagnosis of fecal shedders of MAP without any clinical signs. Based on this situation, attempts were made to identify biomarkers that show early responses to MAP infection in a macrophage cell line, RAW 264.7. In response to the infection with the bacterium, a lot of genes were turned on and/or off in the cells. Of the altered genes, three different categories were identified based on the time-dependent gene expression patterns. Those genes were considered as possible candidates for biomarkers of MAP infection after confirmation by quantitative RT-PCR analysis. To the best of our knowledge, this is the first attempt at discovering the host transcriptomic biomarkers of PTB, although further investigation will be required to determine whether these biomarker candidates are associated within the natural host.
authors.affiliation: Department of Infectious Dsieases, College of Veterinary Medcine, Brain Korea 21 for Veterinary Sceince, Seoul National University, Seoul 151-742, Republic of Korea.
authors.forename: Seung Bin
authors.initials: SB
authors.lastname: Cha
authors.forename: Anna
authors.initials: A
authors.lastname: Yoo
authors.forename: Hong Tae
authors.initials: HT
authors.lastname: Park
authors.forename: Kyoung Yong
authors.initials: KY
authors.lastname: Sung
authors.forename: Min Kyoung
authors.initials: MK
authors.lastname: Shin
authors.forename: Han Sang
authors.initials: HS
authors.lastname: Yoo
mesh_terms: D000818:Animals; D015415:Biomarkers; D002460:Cell Line; D020869:Gene Expression Profiling; D054884:Host-Pathogen Interactions; D008264:Macrophages / Q000382:microbiology*; D051379:Mice; D016927:Mycobacterium avium subsp. paratuberculosis / Q000254:growth & development*; D060888:Real-Time Polymerase Chain Reaction; D013997:Time Factors
chemical_list: D015415:Biomarkers