title: Adaptation to tert-butyl hydroperoxide at a plasma membrane level in the fission yeast Schizosaccharomyces pombe parental strain and its t-BuOOH-resistant mutant.
abstract: The one-gene mutant hyd1-190 of the fission yeast Schizosaccharomyces pombe displayed four-fold resistance to tert-butyl hydroperoxide (t-BuOOH) in comparison with its parental strain hyd(+). The cells of hyd1-190 exhibited a quantitative alteration in the sterol content and hence in the fatty acid composition of the plasma membrane, reflected in a two-fold amphotericin B sensitivity, increased rigidity of the plasma membrane, revealed by an elevated (Δ7.9 °C) phase-transition temperature, measured by means of electron paramagnetic resonance spectroscopy, and a significantly decreased uptake of glycerol. Treatment of the strains with a subinhibitory concentration (0.2 mM) of t-BuOOH induced adaptation via modification of the sterol and fatty acid compositions, resulting in increased (Δ3.95 °C) and decreased (Δ6.83 °C) phase-transition temperatures of the hyd(+) and hyd1-190 strains, respectively, in order to defend the cells against the consequences of t-BuOOH-induced external oxidative stress. However, in contrast with hyd(+), hyd1-190 lacks the ability to adapt to t-BuOOH at a cell level.
authors.affiliation: Department of General and Environmental Microbiology, Faculty of Sciences, University of Pécs, Pécs, Hungary.
authors.forename: Nikoletta
authors.initials: N
authors.lastname: Kálmán
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Gazdag
authors.forename: Milan
authors.initials: M
authors.lastname: Čertík
authors.forename: József
authors.initials: J
authors.lastname: Belágyi
authors.forename: Samy A
authors.initials: SA
authors.lastname: Selim
authors.forename: István
authors.initials: I
authors.lastname: Pócsi
authors.forename: Miklós
authors.initials: M
authors.lastname: Pesti
mesh_terms: D002462:Cell Membrane / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism; D006358:Hot Temperature; D009154:Mutation; D044367:Phase Transition; D012568:Schizosaccharomyces / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D020122:tert-Butylhydroperoxide / Q000378:metabolism* / Q000633:toxicity
chemical_list: D020122:tert-Butylhydroperoxide; D005990:Glycerol
keywords: Oxidative stress; Plasma membrane; Resistance; Tert-butyl hydroperoxide; Yeast