title: LC-MS/MS analysis of palytoxin analogues in blue humphead parrotfish Scarus ovifrons causing human poisoning in Japan.
abstract: Since 1953, a total of 27 human poisoning cases caused by the consumption of blue humphead parrotfish, Scarus ovifrons, have been reported in Japan. Characteristic symptoms are severe muscle pain associated with rhabdomyolysis. Although it is believed that palytoxin, which is one of the most potent non-protein marine biotoxins, is the most likely causative toxin in blue humphead parrotfish poisoning, palytoxin has not been proven conclusively as the causative toxin because of lack of a reliable and sensitive analytical method for palytoxin. In 2011, human poisoning cases caused by the consumption of blue humphead parrotfish occurred in Miyazaki and Tokyo. In our present study, an LC-MS/MS method for palytoxin and its analogues in the blue humphead parrotfish samples causing the human poisoning cases in 2011 was developed and the samples were analysed by using the newly developed LC-MS/MS method. Palytoxin and its analogues were not detected in the samples from the food poisoning cases. The LC-MS/MS findings therefore do not support the recently accepted hypothesis that palytoxin is the causative agent in blue humphead parrotfish poisoning in Japan.
authors.affiliation: National Research Institute of Fisheries Science, Fukuura, Kanazawa-ku, Yokohama, Japan. tsuzuki@affrc.go.jp
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Suzuki
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Watanabe
authors.forename: Ryoji
authors.initials: R
authors.lastname: Matsushima
authors.forename: Kenji
authors.initials: K
authors.lastname: Ishihara
authors.forename: Hajime
authors.initials: H
authors.lastname: Uchida
authors.forename: Saori
authors.initials: S
authors.lastname: Kikutsugi
authors.forename: Tomoko
authors.initials: T
authors.lastname: Harada
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Nagai
authors.forename: Masao
authors.initials: M
authors.lastname: Adachi
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yasumoto
authors.forename: Masakazu
authors.initials: M
authors.lastname: Murata
mesh_terms: D000178:Acrylamides / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D003064:Cnidarian Venoms; D005260:Female; D005399:Fishes / Q000378:metabolism*; D005517:Foodborne Diseases; D006801:Humans; D051379:Mice; D008813:Mice, Inbred ICR; D015394:Molecular Structure; D012206:Rhabdomyolysis / Q000139:chemically induced / Q000209:etiology; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000095702:Polyether Toxins
chemical_list: D000178:Acrylamides; D003064:Cnidarian Venoms; C010272:palytoxin; D000095702:Polyether Toxins