title: Simultaneous determination of chloramphenicol, florfenicol and florfenicol amine in ham sausage with a hybrid chemiluminescent immunoassay.
abstract: A novel chemiluminescent immunoassay utilising two types of primary antibodies (murine monoclonal antibody and rabbit polyclonal antibody) and two types of horseradish peroxidase-labelled secondary antibodies was established for simultaneously detecting multiple amphenicol residues in ham sausage. After combining the extract procedure of the target amphenicol into one simplified method, this hybrid chemiluminescent immunoassay could screen chloramphenicol (CAP), florfenicol (FF) and its metabolite florfenicol amine (FFA) at the same time by adding the corresponding secondary antibody. Ham sausage samples were analysed by using this hybrid immunoassay, with LODs of CAP being 0.01 μg kg⁻¹, of FF being 2.8 μg kg⁻¹ and of FFA being 3.0 μg kg⁻¹. The applicability of the proposed method has been validated by determining CAP, FF and FFA in ham sausage samples with satisfactory results. Good recoveries and high correlation with traditional enzyme-linked immunosorbent assay and LC-MS/MS results illustrated that the developed hybrid chemiluminescent immunoassay could screen high-throughput ultra-trace amphenicol residues effectively at one time.
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing, China.
authors.forename: Xiaoqi
authors.initials: X
authors.lastname: Tao
authors.forename: Haiyang
authors.initials: H
authors.lastname: Jiang
authors.forename: Xuezhi
authors.initials: X
authors.lastname: Yu
authors.forename: Jinghui
authors.initials: J
authors.lastname: Zhu
authors.forename: Xia
authors.initials: X
authors.lastname: Wang
authors.forename: Zhanhui
authors.initials: Z
authors.lastname: Wang
authors.forename: Lanlan
authors.initials: L
authors.lastname: Niu
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Wu
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Shen
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002701:Chloramphenicol / Q000032:analysis*; D007118:Immunoassay / Q000379:methods*; D057230:Limit of Detection; D049449:Luminescence; D008461:Meat Products / Q000032:analysis*; D015203:Reproducibility of Results; D013552:Swine; D013839:Thiamphenicol / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D000900:Anti-Bacterial Agents; D002701:Chloramphenicol; C083312:florfenicol amine; C035534:florfenicol; D013839:Thiamphenicol