title: Protective effects of melatonin against spinal cord injury induced oxidative damage in rat kidney: A morphological and biochemical study.
abstract: Spinal cord injury (SCI) induced oxidative stress affects multiple organ systems including the kidney. We studied the possible protective effects of melatonin on SCI-induced oxidative damage in renal tissues of rats. Wistar albino rats (n = 24) were exposed to SCI and divided into vehicle- or melatonin-treated SCI groups. Melatonin was administred intraperitoneally at a dose of 10 mg/kg for seven days. Renal tissues were investigated by light and electron microscopy. Furthermore, tissue malondialdehyde (MDA) and glutathione (GSH) levels and myeloperoxidase (MPO) and superoxide dismutase (SOD) activities were also determined. In the vehicle-treated SCI group, the renal histology was disturbed compared to controls, whereas the melatonin-treated SCI group showed significantly reduced degeneration of renal tissue as seen by both light and electron microscopy. MDA levels, MPO and SOD activities were increased and GSH levels were decreased in the vehicle-treated SCI group compared to controls. On the other hand, decreased MDA levels and MPO activities and increased GSH levels were observed in the melatonin-treated SCI group compared to vehicle-treated SCI group. These results showed that experimentally induced SCI caused oxidative stress in the rat kidney, whereas melatonin treatment reduced oxidative stress, suggesting that it may be used as a complementary therapy of renal problems occurring following SCI.
authors.affiliation: Department of Histology and Embryology, School of Medicine, Marmara University, Istanbul, Turkey. Electronic address: dilekbangir@yahoo.com.
authors.forename: Dilek
authors.initials: D
authors.lastname: Akakin
authors.forename: Demir
authors.initials: D
authors.lastname: Kiran
authors.forename: Naziye
authors.initials: N
authors.lastname: Ozkan
authors.forename: Mehmet
authors.initials: M
authors.lastname: Erşahin
authors.forename: Zarife Nigar
authors.initials: ZN
authors.lastname: Ozdemir-Kumral
authors.forename: Berrak
authors.initials: B
authors.lastname: Yeğen
authors.forename: Göksel
authors.initials: G
authors.lastname: Sener
mesh_terms: D000818:Animals; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008550:Melatonin / Q000008:administration & dosage / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013119:Spinal Cord Injuries / Q000378:metabolism* / Q000473:pathology
chemical_list: D008550:Melatonin
keywords: CAT; GSH; H&E; Kidney; LPO; MDA; MPO; Melatonin; Oxidative stress; PAS; PBS; ROS; SCI; SOD; Spinal cord injury; TEM; Ultrastructure; catalase; glutathione; hematoxylin and eosin; lipid peroxidation; malondialdehyde; myeloperoxidase; periodic acid-schiff; phosphate buffered saline; reactive oxygen species; spinal cord injury; superoxide dismutase; transmission electron microscope