title: MicroRNA expression differs in cutaneous squamous cell carcinomas and healthy skin of immunocompetent individuals.
abstract: Cutaneous squamous cell carcinoma (cSCC) is one of the most common skin cancers, but the influence of microRNA (miRNA) expression has only been sporadically analysed. We hypothesized that miRNAs are differentially expressed in cSCC and hence influence its development. We therefore isolated total miRNA from well-differentiated cSCCs and from controls without SCC. Expression analyses of 12 miRNAs showed three significantly differentially expressed miRNAs. We identified a significant upregulation of the miR-21 and the miR-31, a proto-oncogene like miR-21. While the upregulated expression of miR-21 has been known for some time, the increased expression of miR-31 was never shown so clearly. Furthermore, we showed the upregulation of miRNA-205, which has never been described before. The miR-205 induces specific keratinocyte migration and could be a characteristic marker for cSCC. It has to be determined in following studies whether these upregulated expressions are specific for cSCC and if so, for which cSCC stages.
authors.forename: Christelle
authors.initials: C
authors.lastname: Bruegger
authors.forename: Werner
authors.initials: W
authors.lastname: Kempf
authors.forename: Iris
authors.initials: I
authors.lastname: Spoerri
authors.forename: Andreas W
authors.initials: AW
authors.lastname: Arnold
authors.forename: Peter H
authors.initials: PH
authors.lastname: Itin
authors.forename: Bettina
authors.initials: B
authors.lastname: Burger
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000378:metabolism*; D016022:Case-Control Studies; D002454:Cell Differentiation; D002465:Cell Movement; D049109:Cell Proliferation; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D015603:Keratinocytes / Q000166:cytology; D035683:MicroRNAs / Q000378:metabolism*; D000090063:Proto-Oncogene Mas; D012867:Skin / Q000378:metabolism*; D012878:Skin Neoplasms / Q000378:metabolism*; D015854:Up-Regulation
chemical_list: D035683:MicroRNAs; D000090063:Proto-Oncogene Mas; C000715470:MAS1 protein, human; C534425:MIR205, human; C524940:MIRN21 microRNA, human; C534612:MIRN31 microRNA, human