title: Purification, crystallization and preliminary X-ray analysis of the strawberry allergens Fra a 1E and Fra a 3 in the presence of catechin.
abstract: The strawberry Fra a proteins belong to the pathogenesis-related PR-10 protein family and share a common fold with the Bet v 1 major pollen allergen and the START/PYR/PYL proteins, which are characterized by the presence of a central cavity and are often involved in the binding of a variety of natural compounds. The Fra a proteins play a key role in the control of flavonoid biosynthesis in strawberries and are essential for pigment formation in fruits. In order to understand Fra a protein function, full-length Fra a 1E and Fra a 3 cDNAs were cloned and expressed in Escherichia coli, and the proteins were purified to homogeneity using metal-affinity chromatography. Diffraction-quality crystals of Fra a 1E and of Fra a 3 in the presence of (+)-catechin were obtained by the sitting-drop vapour-diffusion method. X-ray diffraction data from single crystals of Fra a 1E and Fra a 3 were processed to 2.2 and 3.0 Å resolution in space groups P212121 and P2221, with unit-cell parameters a = 70.02, b = 74.42, c = 84.04 Å and a = 137.91, b = 206.61, c = 174.7 Å for Fra a 1E and Fra a 3, respectively.
authors.affiliation: Instituto de Hortofruticultura Subtropical y Mediterránea (IHSM-UMA-CSIC), Departamento de Biología Molecular y Bioquímica, Universidad de Málaga, 29071 Málaga, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Casañal
authors.forename: Ulrich
authors.initials: U
authors.lastname: Zander
authors.forename: Florine
authors.initials: F
authors.lastname: Dupeux
authors.forename: Victoriano
authors.initials: V
authors.lastname: Valpuesta
authors.forename: Jose A
authors.initials: JA
authors.lastname: Marquez
mesh_terms: D000485:Allergens / Q000737:chemistry / Q000302:isolation & purification; D052179:Antigens, Plant / Q000737:chemistry* / Q000302:isolation & purification; D002392:Catechin / Q000737:chemistry / Q000302:isolation & purification; D003460:Crystallization; D031985:Fragaria; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification; D014961:X-Ray Diffraction
chemical_list: D000485:Allergens; D052179:Antigens, Plant; C517508:Fra a 1 allergen, Fragaria ananassa; C514339:Fra a 3 allergen, Fragaria vesca; D010940:Plant Proteins; D002392:Catechin
keywords: Fra a 1E; Fra a 3; PR-10 proteins; catechin