title: An in-depth comparison of the male pediatric and adult urinary proteomes.
abstract: In this study, we performed an in-depth characterization of the male pediatric infant urinary proteome by parallel proteomic analysis of normal healthy adult (n=6) and infant (n=6) males and comparison to available published data. A total of 1584 protein groups were identified. Of these, 708 proteins were identified in samples from both cohorts. Although present in both cohorts, 136 of these common proteins were significantly enriched in urine from adults and 94 proteins were significantly enriched in urine from infants. Using Gene Ontology, we found that the infant-enriched or specific subproteome (743 proteins) had an overrepresentation of proteins that are involved in translation and transcription, cellular growth and metabolic processes. In contrast, the adult enriched or specific subproteome (364 proteins) showed an overexpression of proteins involved in immune response and cell adhesion. This study demonstrates that the non-diseased male urinary proteome is quantitatively affected by age, has age-specific subproteomes, and identifies a common subproteome with no age-dependent abundance variations. These findings highlight the importance of age-matching in urinary proteomics. This article is part of a Special Issue entitled: Biomarkers: A Proteomic Challenge.
authors.affiliation: Boston Children's Hospital, Urological Diseases Research Center, Department of Urology, Harvard Medical School, Boston, MA, USA; Proteomics Center at Boston Children's Hospital, Boston, MA, USA.
authors.forename: John W
authors.initials: JW
authors.lastname: Froehlich
authors.affiliation: Boston Children's Hospital, Urological Diseases Research Center, Department of Urology, Harvard Medical School, Boston, MA, USA; Proteomics Center at Boston Children's Hospital, Boston, MA, USA.
authors.forename: Ali R
authors.initials: AR
authors.lastname: Vaezzadeh
authors.affiliation: Department of Pathology, Boston Children's Hospital and Harvard Medical School, Boston, MA, USA; Proteomics Center at Boston Children's Hospital, Boston, MA, USA.
authors.forename: Marc
authors.initials: M
authors.lastname: Kirchner
authors.affiliation: Boston Children's Hospital, Urological Diseases Research Center, Department of Urology, Harvard Medical School, Boston, MA, USA; Proteomics Center at Boston Children's Hospital, Boston, MA, USA.
authors.forename: Andrew C
authors.initials: AC
authors.lastname: Briscoe
authors.affiliation: Department of Biostatistics, Harvard School of Public Health, Boston, MA, USA.
authors.forename: Oliver
authors.initials: O
authors.lastname: Hofmann
authors.affiliation: Department of Biostatistics, Harvard School of Public Health, Boston, MA, USA.
authors.forename: Winston
authors.initials: W
authors.lastname: Hide
authors.affiliation: Department of Pathology, Boston Children's Hospital and Harvard Medical School, Boston, MA, USA; Proteomics Center at Boston Children's Hospital, Boston, MA, USA. Electronic address: hanno.steen@childrens.harvard.edu.
authors.forename: Hanno
authors.initials: H
authors.lastname: Steen
authors.affiliation: Boston Children's Hospital, Urological Diseases Research Center, Department of Urology, Harvard Medical School, Boston, MA, USA; Proteomics Center at Boston Children's Hospital, Boston, MA, USA. Electronic address: richard.lee@childrens.harvard.edu.
authors.forename: Richard S
authors.initials: RS
authors.lastname: Lee
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000652:urine*; D002853:Chromatography, Liquid; D015331:Cohort Studies; D006801:Humans; D007223:Infant; D008297:Male; D011506:Proteins / Q000032:analysis*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D013347:Subcellular Fractions; D053719:Tandem Mass Spectrometry; D014556:Urine / Q000737:chemistry*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D011506:Proteins; D020543:Proteome
keywords: Mass spectrometry; Pediatric urinary proteome; Spectral counting; Urinary proteomics