title: Replacing amino acids in translation: expanding chemical diversity with non-natural variants.
abstract: Here, we describe a strategy for synthesis of peptides with multiple unnatural amino acids (UAAs) using in vitro translation. Our method involves removing a natural amino acid and replacing it with an UAA variant in a reconstituted translation system. Whereas other systems require engineered components or chemical synthesis to charge UAAs onto tRNAs prior to translation, our strategy utilizes the wild-type machinery and charging occurs concomitant with translation. The design of the system allows for easy quantification of the UAA's incorporation efficiency and fidelity.
authors.affiliation: Department of Chemistry and Massey Cancer Center, Virginia Commonwealth University, 401 College Street Richmond, VA 23298-0037, USA.
authors.forename: E Railey
authors.initials: ER
authors.lastname: White
authors.forename: Timothy M
authors.initials: TM
authors.lastname: Reed
authors.forename: Zhong
authors.initials: Z
authors.lastname: Ma
authors.forename: Matthew C T
authors.initials: MC
authors.lastname: Hartman
mesh_terms: D000596:Amino Acids / Q000737:chemistry / Q000235:genetics; D014644:Genetic Variation; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000235:genetics; D014176:Protein Biosynthesis; D016337:RNA, Catalytic / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000596:Amino Acids; D010455:Peptides; D016337:RNA, Catalytic