title: Pin1-Nanog expression in human glioma is correlated with advanced tumor progression.
abstract: The stemness gene Nanog has been shown to play an important role in tumor development, including glioma. Nanog is phosphorylated at multiple Ser/Thr-Pro motifs, which promotes the interaction between Nanog and the prolyl isomerase Pin1, leading to Nanog stabilization by suppressing its ubiquitination. The present study investigated the expression and relationship of Pin1 and Nanog in human gliomas. Significantly higher mRNA and protein expression levels of Pin1 and Nanog were demonstrated in 120 glioma specimens of different pathological grades by RT-PCR, immunohistochemistry staining and western blot analysis. The relative levels of Pin1 expression, as well as Nanog expression, were significantly positively correlated with pathological grade. Moreover, a positive correlation of Pin1 and Nanog expression in human gliomas was noted. Co-localization of Pin1 and Nanog was observed in the perinuclear space in the cytoplasm of glioma cells detected by immunofluorescence staining. Significantly positive correlation between Pin1 and Nanog in gliomas indicated that Pin1 and Nanog may be related to tumorigenesis and development of glioma cells.
authors.affiliation: Department of Neurosurgery, Anhui Provincial Hospital Affiliated to Anhui Medical University, Hefei, Anhui 230001, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.forename: Chao-Shi
authors.initials: CS
authors.lastname: Niu
authors.forename: Chuan-Dong
authors.initials: CD
authors.lastname: Cheng
mesh_terms: D045744:Cell Line, Tumor; D003593:Cytoplasm / Q000235:genetics / Q000473:pathology; D018450:Disease Progression; D005910:Glioma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D018398:Homeodomain Proteins / Q000096:biosynthesis* / Q000235:genetics; D006801:Humans; D000072340:NIMA-Interacting Peptidylprolyl Isomerase; D000071317:Nanog Homeobox Protein; D019696:Peptidylprolyl Isomerase / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
chemical_list: D018398:Homeodomain Proteins; C486780:NANOG protein, human; D000072340:NIMA-Interacting Peptidylprolyl Isomerase; D000071317:Nanog Homeobox Protein; D012333:RNA, Messenger; C000606846:PIN1 protein, human; D019696:Peptidylprolyl Isomerase