title: Interactions between indole-3-acetic acid (IAA) with a lectin from Canavalia maritima seeds reveal a new function for lectins in plant physiology.
abstract: Indole-3-acetic acid (IAA) bound is considered a storage molecule and is inactive. However, some studies have proposed an additional possible regulatory mechanism based on the ability of lectins to form complexes with IAA. We report the first crystal structure of ConM in complex with IAA at 2.15 Å resolution. Based on a tetrameric model of the complex, we hypothesize how the lectin controls the availability of IAA during the early seedling stages, indicating a possible new physiological role for these proteins. A free indole group is also bound to the protein. The ConM interaction with different forms of IAA is a strategy to render the phytohormone unavailable to the cell. Thus, this new physiological role proposed for legume lectins might be a novel mechanism by which IAA levels are decreased in addition to the destruction and formation of new complexes in the later stages of seed germination.
authors.affiliation: Departamento de Biologia Molecular, Universidade Federal da Paraíba, João Pessoa, Brazil. pldelatorre@gmail.com
authors.forename: Plinio
authors.initials: P
authors.lastname: Delatorre
authors.forename: José Caetano
authors.initials: JC
authors.lastname: Silva-Filho
authors.forename: Bruno Anderson Matias
authors.initials: BA
authors.lastname: Rocha
authors.forename: Tatiane
authors.initials: T
authors.lastname: Santi-Gadelha
authors.forename: Raphael Batista
authors.initials: RB
authors.lastname: da Nóbrega
authors.forename: Carlos Alberto Almeida
authors.initials: CA
authors.lastname: Gadelha
authors.forename: Kyria Santiago
authors.initials: KS
authors.lastname: do Nascimento
authors.forename: Celso Shiniti
authors.initials: CS
authors.lastname: Nagano
authors.forename: Alexandre Holanda
authors.initials: AH
authors.lastname: Sampaio
authors.forename: Benildo Sousa
authors.initials: BS
authors.lastname: Cavada
mesh_terms: D000818:Animals; D032449:Canavalia / Q000378:metabolism / Q000502:physiology*; D006384:Hemagglutination / Q000187:drug effects; D007210:Indoleacetic Acids / Q000378:metabolism*; D062105:Molecular Docking Simulation; D037121:Plant Lectins / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D011485:Protein Binding; D011487:Protein Conformation; D011817:Rabbits; D012639:Seeds / Q000378:metabolism*
chemical_list: D007210:Indoleacetic Acids; D037121:Plant Lectins; C030737:indoleacetic acid
keywords: Canavalia maritima; Indole-acetic acid; Lectin