title: Genotoxic effects of exposure to waterborne uranium, dietary methylmercury and hyperoxia in zebrafish assessed by the quantitative RAPD-PCR method.
abstract: Release of chemicals and fluctuation in oxygen content in the aquatic environment represent hazards for fish health. The present study aims at assessing the genotoxic impact of low concentration exposures to waterborne uranium (U), dietary methyl mercury (MeHg) and hyperoxia in zebrafish by using the RAPD-PCR quantitative method. A significant increase of the number hybridization sites was observed in fish exposed to 30μgU/L and 100μgU/L and hyperoxia. In fish exposed to MeHg (13.5μg Hg/g, dry weight) no change in the number of hybridization sites were found, however, the frequency of PCR products showed significant variation. The mechanisms of toxicity leading to DNA damage in fish exposed to waterborne uranium, mercury and hyperoxia are discussed and the results from the literature given by the comet assay, micronucleus test and RAPD-PCR method compared. The study provides new data regarding the genotoxic effects of MeHg, hyperoxia and low U concentrations (30μgU/L) in fish. The present work highlights the use of the RAPD-PCR as a sensitive method in the assessment of chemically-induced DNA damage in animals.
authors.affiliation: Institut de Radioprotection et de Sûreté Nucléaire, Saint-Paul-Lez-Durance Cedex, France.
authors.forename: Adélaïde
authors.initials: A
authors.lastname: Lerebours
authors.forename: Sébastien
authors.initials: S
authors.lastname: Cambier
authors.forename: Lindsay
authors.initials: L
authors.lastname: Hislop
authors.forename: Christelle
authors.initials: C
authors.lastname: Adam-Guillermin
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Bourdineaud
mesh_terms: D000818:Animals; D004249:DNA Damage / Q000187:drug effects*; D004032:Diet; D008767:Methylmercury Compounds / Q000032:analysis* / Q000633:toxicity; D015162:Micronucleus Tests; D010100:Oxygen / Q000633:toxicity*; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D014501:Uranium / Q000032:analysis* / Q000633:toxicity; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000633:toxicity; D015027:Zebrafish / Q000502:physiology*
chemical_list: D008767:Methylmercury Compounds; D014874:Water Pollutants, Chemical; D014501:Uranium; C073972:dimethyl mercury; D010100:Oxygen
keywords: DNA damage; Environmental stressors; Fish; Probe's hybridization sites