title: [Preliminary study of akermanite's effect on stem cells from goat deciduous teeth in proliferation and osteogenic differentiation].
abstract: OBJECTIVE
  To explore the characters of attachment, proliferation and osteogenic differentiation of stem cells from goat deciduous teeth (SGDs) cultured on akermanite ceramic.
  
  
  METHODS
  SGDs were collected with enzyme digestion, and cultured. Under culture condition in growth medium, actin filament labeling and CCK-8 analysis were used to reveal attachment and proliferation. Expression level of alkaline phosphatase (ALP) and osteogenic differentiation genes (ALP, COL I, OPN and OCN) were examined by pNPP method and real-time PCR. The data was analyzed using SPSS 13.0 software package.
  
  
  RESULTS
  Compared with β-TCP, SGDs seeded on akermanite ceramic not only spread out better, but also grew faster since day 4. In osteogenic medium, cells of akermanite group had stronger ALP activity at day 4 and 7. The gene expression of akermanite was more than the β-TCP group. The gene OCN expression level increased significantly at day 7 (P<0.01).
  
  
  CONCLUSIONS
  SGDs can attach and grow well on akermanite ceramic. Compared with β-TCP, akermanite ceramic shows an advantage in osteogenic differentiation of SGDs.
authors.affiliation: Department of Stomatology, Shanghai Jiao Tong University, Shanghai, China. bookswallower@gmail.com
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
authors.forename: Jia-yu
authors.initials: JY
authors.lastname: Lu
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.forename: Yong-ming
authors.initials: YM
authors.lastname: Hao
authors.forename: Jia
authors.initials: J
authors.lastname: Yu
authors.forename: Cheng-qi
authors.initials: CQ
authors.lastname: Lv
authors.forename: De-rong
authors.initials: DR
authors.lastname: Zou
mesh_terms: D000469:Alkaline Phosphatase; D000818:Animals; D002130:Calcium Phosphates; D002454:Cell Differentiation; D049109:Cell Proliferation; D002516:Ceramics; D006041:Goats; D010012:Osteogenesis; D013234:Stem Cells; D014094:Tooth, Deciduous
chemical_list: D002130:Calcium Phosphates; C485817:beta-tricalcium phosphate; C023506:Glass ceramics; D000469:Alkaline Phosphatase