title: Development of an in vitro thin-film solid-phase microextraction method to determine the bioavailability of xenoestrogens in soil.
abstract: Biosolids applied to agricultural fields, parks, and other areas represent significant sources of estrogen-like endocrine disrupting compound (EEDC) inputs to soil. It is important to determine the bioavailability of EEDCs in soil to inform risk assessment concerning their environmental presence; Eisenia fetida (earthworms) are typically used in traditional in vivo bioavailability experiments. The development of an in vitro bioavailability method will decrease time, expense, and use of solvents in future analyses. A thin-film solid-phase microextraction (TF-SPME) method for determining the bioavailability of several EEDCs detected in biosolids was developed and optimized. It was found that the TF-SPME method could be used to calculate equilibrium porewater concentrations of diethylhexyl phthalate, bisphenol A, benzophenone, and triclosan at environmentally relevant concentrations in artificial soil within 88 min. The potential and limitations of using TF-SPME-generated porewater concentrations to predict E. fetida tissue concentrations are discussed.
authors.affiliation: Graduate Field of Environmental Toxicology, Department of Fiber Science and Apparel Design, Cornell University, Ithaca, New York, USA.
authors.forename: Katherine Neafsey
authors.initials: KN
authors.lastname: Engler
authors.forename: Ann T
authors.initials: AT
authors.lastname: Lemley
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000032:analysis; D001577:Benzophenones / Q000032:analysis; D004051:Diethylhexyl Phthalate / Q000032:analysis; D052244:Endocrine Disruptors / Q000032:analysis*; D004784:Environmental Monitoring; D004967:Estrogens / Q000032:analysis*; D060587:Groundwater / Q000737:chemistry; D009835:Oligochaeta / Q000737:chemistry; D010636:Phenols / Q000032:analysis; D012987:Soil / Q000737:chemistry*; D012989:Soil Pollutants / Q000032:analysis*; D052617:Solid Phase Microextraction / Q000379:methods*; D014260:Triclosan / Q000032:analysis; D014874:Water Pollutants, Chemical / Q000032:analysis; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D001577:Benzophenones; D004051:Diethylhexyl Phthalate; D052244:Endocrine Disruptors; D004967:Estrogens; D010636:Phenols; D012987:Soil; D012989:Soil Pollutants; D014260:Triclosan; D014874:Water Pollutants, Chemical; C047723:benzophenone; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bioavailability; Eisenia fetida; Solid-phase microextraction; Xenoestrogen