title: An ultrasensitive luminol cathodic electrochemiluminescence immunosensor based on glucose oxidase and nanocomposites: graphene-carbon nanotubes and gold-platinum alloy.
abstract: In the present study, a novel and ultrasensitive electrochemiluminescence (ECL) immunosensor based on luminol cathodic ECL was fabricated by using Au nanoparticles and Pt nanoparticles (nano-AuPt) electrodeposited on graphene-carbon nanotubes nanocomposite as platform for the detection of carcinoembryonic antigen (CEA). For this introduced immunosensor, graphene (GR) and single wall carbon nanotubes (CNTs) dispersed in chitosan (Chi-GR-CNTs) were firstly decorated on the bare gold electrode (GE) surface. Then nano-AuPt were electrodeposited (DpAu-Pt) on the Chi-GR-CNTs modified electrode. Subsequently, glucose oxidase (GOD) was employed to block the non-specific sites of electrode surface. When glucose was present in the working buffer solution, GOD immediately catalyzed the oxidation of glucose to in situ generate hydrogen peroxide (H2O2), which could subsequently promote the oxidation of luminol with an amplified cathodic ECL signal. The proposed immunosensor was performed at low potential (-0.1 to 0.4V) and low concentration of luminol. The CEA was determined in the range of 0.1 pg mL(-1) to 40 ng mL(-1) with a limit of detection down to 0.03 pg mL(-1) (SN(-1)=3). Moreover, with excellent sensitivity, selectivity, stability and simplicity, the as-proposed luminol-based ECL immunosensor provided great potential in clinical applications.
authors.affiliation: Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China. j112008@163.com
authors.forename: Xinya
authors.initials: X
authors.lastname: Jiang
authors.forename: Yaqin
authors.initials: Y
authors.lastname: Chai
authors.forename: Ruo
authors.initials: R
authors.lastname: Yuan
authors.forename: Yaling
authors.initials: Y
authors.lastname: Cao
authors.forename: Yingfeng
authors.initials: Y
authors.lastname: Chen
authors.forename: Haijun
authors.initials: H
authors.lastname: Wang
authors.forename: Xianxue
authors.initials: X
authors.lastname: Gan
mesh_terms: D000497:Alloys / Q000737:chemistry*; D002272:Carcinoembryonic Antigen / Q000032:analysis; D004566:Electrodes; D005949:Glucose Oxidase / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D008163:Luminescent Measurements / Q000379:methods*; D008165:Luminol / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry*
chemical_list: D000497:Alloys; D002272:Carcinoembryonic Antigen; D037742:Nanotubes, Carbon; D010984:Platinum; D008165:Luminol; D006046:Gold; D006108:Graphite; D005949:Glucose Oxidase