title: Incidence of micronuclei in human peripheral blood lymphocytes exposed to modulated and unmodulated 2450 MHz radiofrequency fields.
abstract: Peripheral blood samples from four healthy volunteers were collected and aliquots were exposed in vitro for 2 h to either (i) modulated (wideband code division multiple access, WCDMA) or unmodulated continuous wave (CW) 2450 MHz radiofrequency (RF) fields at an average specific absorption rate of 10.9 W/kg or (ii) sham-exposed. Aliquots of the same samples that were exposed in vitro to an acute dose of 1.5 Gy ionizing gamma-radiation (GR) were used as positive controls. Half of the aliquots were treated with melatonin (Mel) to investigate if such treatment offers protection to the cells from the genetic damage, if any, induced by RF and GR. The cells in all samples were cultured for 72 h and the lymphocytes were examined to determine the extent of genetic damage assessed from the incidence of micronuclei (MN). The results indicated the following: (i) the incidence of MN was similar in incubator controls, and those exposed to RF/sham and Mel alone; (ii) there were no significant differences between WCDMA and CW RF exposures; (iii) positive control cells exposed to GR alone exhibited significantly increased MN; and (iv) Mel treatment had no effect on cells exposed to RF and sham, while such treatment significantly reduced the frequency of MN in GR-exposed cells.
authors.affiliation: Department of Radiology, University of Texas Health Science Center, San Antonio, Texas; Brain and Psychological Sciences Research Centre, Swinburne University of Technology, Melbourne, Victoria, Australia.
authors.lastname: Vijayalaxmi
authors.forename: Abhishek B
authors.initials: AB
authors.lastname: Reddy
authors.forename: Raymond J
authors.initials: RJ
authors.lastname: McKenzie
authors.forename: Robert L
authors.initials: RL
authors.lastname: McIntosh
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Prihoda
authors.forename: Andrew W
authors.initials: AW
authors.lastname: Wood
mesh_terms: D000368:Aged; D040421:Cell Phone; D005260:Female; D005720:Gamma Rays / Q000009:adverse effects; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects*; D008297:Male; D008550:Melatonin / Q000494:pharmacology; D015162:Micronucleus Tests; D008875:Middle Aged; D011846:Radio Waves / Q000009:adverse effects*
chemical_list: D008550:Melatonin
keywords: WCDMA; binucleate cells; genotoxicity; melatonin; micronuclei