title: Pseudomonas asturiensis sp. nov., isolated from soybean and weeds.
abstract: Five strains of gram negative bacteria, isolated from soybean (LPPA 221(T), 222 and 223) and weeds (LPPA 816 and 1442), were analyzed by a polyphasic approach. The isolates showed variation in their phenotypic traits and were placed in the Pseudomonas fluorescens lineage, based on 16S rRNA gene sequence phylogeny, as a single but well separated cluster. MLSA analysis based on gyrB and rpoD sequences clustered the strains in a single branch in the Pseudomonas syringae group, and revealed P. viridiflava as closest relative. DNA-DNA hybridizations showed medium levels of DNA-DNA relatedness with the type strain of P. viridiflava (50%) and lower levels (<32%) with other type strains of the P. syringae group, supporting classification within a novel species of the genus Pseudomonas. The strains can be distinguished from species of the P. syringae group by the fatty acid C17:0 cyclo that is present in a low amount (2.5%) and from P. viridiflava by their inability to assimilate d-tartrate and d-sorbitol, and by the formation of red colonies on TTC medium. For this new species, the name Pseudomonas asturiensis sp. nov. is proposed. The type strain is LPPA 221(T) (=LMG 26898(T)=CECT 8095(T)).
authors.affiliation: Servicio Regional de Investigación y Desarrollo Agroalimentario SERIDA, Principado de Asturias, 33300 Villaviciosa, Spain. anagf@serida.org
authors.forename: Ana J
authors.initials: AJ
authors.lastname: González
authors.forename: Ilse
authors.initials: I
authors.lastname: Cleenwerck
authors.forename: Paul
authors.initials: P
authors.lastname: De Vos
authors.forename: Ana M
authors.initials: AM
authors.lastname: Fernández-Sanz
mesh_terms: D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D057685:Plant Weeds / Q000382:microbiology*; D011549:Pseudomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012808:Sigma Factor / Q000235:genetics; D013025:Glycine max / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012808:Sigma Factor; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases; D027081:DNA Gyrase