title: When yeast cells meet, karyogamy!: an example of nuclear migration slowly resolved.
abstract: Cytoskeleton-mediated transport processes are central to the subcellular organization of cells. The nucleus constitutes the largest organelle of a cell, and studying how it is positioned and moved around during various types of cell morphogenetic processes has puzzled researchers for a long time. Now, the molecular architectures of the underlying dynamic processes start to reveal their secrets.   In yeast, karyogamy denotes the migration of two nuclei toward each other-termed nuclear congression-upon partner cell mating and the subsequent fusion of these nuclei to form a diploid nucleus. It constitutes a well-studied case. Recent insights completed the picture about the molecular processes involved and provided us with a comprehensive model amenable to quantitative computational simulation of the process. This review discusses our understanding of yeast nuclear congression and karyogamy and seeks to explain how a detailed, quantitative and systemic understanding has emerged from this knowledge.
authors.affiliation: European Molecular Biology Laboratory EMBL, Heidelberg, Germany.
authors.forename: Romain
authors.initials: R
authors.lastname: Gibeaux
authors.forename: Michael
authors.initials: M
authors.lastname: Knop
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000235:genetics* / Q000648:ultrastructure; D004171:Diploidy; D015966:Gene Expression Regulation, Fungal; D016547:Kinesins / Q000235:genetics* / Q000378:metabolism; D008868:Microtubule Proteins / Q000235:genetics / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000235:genetics* / Q000378:metabolism; D008870:Microtubules / Q000235:genetics* / Q000648:ultrastructure; D008954:Models, Biological; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: C076025:CIK1 protein, S cerevisiae; C063504:KAR3 protein, S cerevisiae; D008868:Microtubule Proteins; D008869:Microtubule-Associated Proteins; D029701:Saccharomyces cerevisiae Proteins; D016547:Kinesins
keywords: Kar3; karyogamy; kinesin-14; microtubule dynamics; microtubule motor protein; nuclear fusion; nuclear migration; spindle pole body; yeast cell morphogenesis; yeast mating