title: Improving the effectiveness of artificial microRNA (amiR)-mediated resistance against Turnip mosaic virus by combining two amiRs or by targeting highly conserved viral genomic regions.
abstract: A drawback of recent antiviral therapies based on the transgenic expression of artificial microRNAs (amiRs) is the ease with which viruses generate escape mutations. Here, we show two alternative strategies for improving the effectiveness of resistance in plants. First, we expressed two amiRs complementary to independent targets in the viral genome, and second, we designed amiRs complementary to highly conserved RNA motifs in the viral genome.
authors.affiliation: Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas-Universitat Politècnica de València, València, Spain.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Lafforgue
authors.forename: Fernando
authors.initials: F
authors.lastname: Martínez
authors.forename: Qi-Wen
authors.initials: QW
authors.lastname: Niu
authors.forename: Nam-Hai
authors.initials: NH
authors.lastname: Chua
authors.forename: José-Antonio
authors.initials: JA
authors.lastname: Daròs
authors.forename: Santiago F
authors.initials: SF
authors.lastname: Elena
mesh_terms: D017360:Arabidopsis; D001499:Bayes Theorem; D017124:Conserved Sequence / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics*; D016679:Genome, Viral / Q000235:genetics*; D016014:Linear Models; D016015:Logistic Models; D035683:MicroRNAs / Q000235:genetics*; D010935:Plant Diseases / Q000517:prevention & control* / Q000821:virology*; D017800:Potyvirus / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D035683:MicroRNAs