title: Diphenyl diselenide supplementation reduces biochemical alterations associated with oxidative stress in rats fed with fructose and hydrochlorothiazide.
abstract: The study evaluated whether a diet containing diphenyl diselenide (PhSe)2, a synthetic antioxidant, could reduce the biochemical alterations induced by chronic consumption of highly enriched fructose diet and/or hydrochlorothiazide (HCTZ). Rats were fed a control diet (CT) or a high fructose diet (HFD), supplemented with or not HCTZ (4.0g/kg) and/or (PhSe)2 (3ppm) for 18weeks. HFD intake increased significantly plasma glucose, fructosamine, triglycerides and cholesterol levels. (PhSe)2 supplementation significantly reduced triglycerides and cholesterol but could not restore them to control levels. The combination of HFD and HCTZ significantly altered plasma glucose, fructosamine, triglycerides and cholesterol levels which were not restore by (PhSe)2 supplementation. Lipid peroxidation, protein carbonyl formation, vitamin C level and catalase activity decreased after HFD, HCTZ or HFD plus HCTZ ingestion. Remarkably (PhSe)2 supplementation restored the oxidative stress parameters. HCTZ decreased renal superoxide dismutase (SOD) activity, which was restored to control levels by (PhSe)2. Furthermore, the association of HFD and HCTZ decreased plasma potassium levels and aggravated HCTZ-induced hypomagnesemia and hypertriglyceridemia. Here we provided evidence of the involvement of oxidative stress and metabolic disorders in a rat model of HFD associated or not with HTCZ. (PhSe)2 supplementation reduced the oxidative stress and this compound should be considered for the treatment of biochemical disturbances and oxidative stress in other animal models of metabolic disorders.
authors.affiliation: Hospital Universitário de Santa Maria,Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil.
authors.forename: Marinei Cristina Pereira
authors.initials: MC
authors.lastname: Ribeiro
authors.forename: Daiana Silva
authors.initials: DS
authors.lastname: Avila
authors.forename: Viviane Patrícia Pires
authors.initials: VP
authors.lastname: Schiar
authors.forename: Danúbia Bonfanti Dos
authors.initials: DB
authors.lastname: Santos
authors.forename: Daiane F
authors.initials: DF
authors.lastname: Meinerz
authors.forename: Marta Medeiros Frescura
authors.initials: MM
authors.lastname: Duarte
authors.forename: Roger
authors.initials: R
authors.lastname: Monteiro
authors.forename: Robson
authors.initials: R
authors.lastname: Puntel
authors.forename: Andreza Fabro
authors.initials: AF
authors.lastname: de Bem
authors.forename: Waseem
authors.initials: W
authors.lastname: Hassan
authors.forename: Nilda Berenice
authors.initials: NB
authors.lastname: de Vargas Barbosa
authors.forename: João Batista Teixeira
authors.initials: JB
authors.lastname: Rocha
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001555:Benzene Derivatives / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D004032:Diet; D019587:Dietary Supplements; D015536:Down-Regulation / Q000187:drug effects; D005632:Fructose / Q000378:metabolism*; D006852:Hydrochlorothiazide / Q000378:metabolism*; D008297:Male; D016566:Organoselenium Compounds / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D001555:Benzene Derivatives; D016566:Organoselenium Compounds; D006852:Hydrochlorothiazide; C061132:diphenyldiselenide; D005632:Fructose; D002374:Catalase
keywords: Diphenyl diselenide; High fructose diet; Hydrochlorothiazide; Oxidative stress