title: Binding of cationic single-chain and dimeric surfactants to bovine serum albumin.
abstract: The interactions between bovine serum albumin (BSA) and the single-chain surfactants N-benzyl-N,N-dimethyl-N-(1-dodecyl)ammonium bromide (PH12) and N-cyclohexylmethyl-N,N-dimethyl-N-(1-dodecyl)ammonium bromide (CH12) and their two dimeric counterparts, N,N'-[1,3-phenylenebis(methylene)]bis[N,N-dimethyl-N-(1-dodecyl)]ammonium dibromide (12PH12) and N,N'-[cyclohexane-1,3-diylbis(methylene)]bis[N,N-dimethyl-N-(1-dodecyl)]ammonium dibromide (12CH12), respectively, have been investigated by surface tension, fluorescence, circular dichroism, ζ potential, and atomic force microscopy. The results obtained permit the examination of the way an increase in the number of hydrophobic chains and the substitution of a cyclohexyl ring by a phenyl ring, either in the headgroup of single-chain surfactants or in the spacer of dimeric surfactants, affect BSA-surfactant interactions. The comparison of the fluorescence results with those obtained by ζ potential measurements shows that the sites of binding of the surfactants with aromatic rings to the BSA are somewhat different from those of the surfactants with no aromatic rings.
authors.affiliation: Department of Physical Chemistry, University of Seville, C/Profesor García González 1, 41012 Seville, Spain.
authors.forename: Victoria Isabel
authors.initials: VI
authors.lastname: Martín
authors.forename: Amalia
authors.initials: A
authors.lastname: Rodríguez
authors.forename: Alfredo
authors.initials: A
authors.lastname: Maestre
authors.forename: María Luisa
authors.initials: ML
authors.lastname: Moyá
mesh_terms: D000818:Animals; D002412:Cations; D002417:Cattle; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry*
chemical_list: D002412:Cations; D013501:Surface-Active Agents; D012710:Serum Albumin, Bovine