title: Spasmolytic effect of curcumin on goat ruminal artery is endothelium independent and by activation of sGC.
abstract: The aim of the present work was to study the mechanism of action of curcumin in vasomotion of a physiologically important artery of ruminant i.e. ruminal artery. ACh and SNP were used to study the role of endothelium in relaxation of this artery. Vasorelaxatation by curcumin was studied in a dose dependent manner, on rings precontracted with 5-hydroxy tryptamine and noradrenalin, in presence and absence of L-NAME, 4AP, ODQ and 4AP+ODQ combination. SNP (1 ηM-100 μM) produced a significant relaxation compared to ACh (0.1-100 μM) on 5-HT (10 μM) and NA (10 μM) induced contraction in endothelium intact rings. Curcumin (10 ηM-100 μM) relaxed the vascular rings in dose dependent manner with maximal relaxation up to 20.94% and 13.81% in 5-HT and NA induced contraction, respectively which was potently blocked by ODQ (10 μM) and combination of 4AP and ODQ (10 μM) but 4AP (10 μM) and L-NAME (100 μM) alone could not block the relaxation and interestingly we observed a slight increase in the tension at higher dose of the agonist (>10 μM). Therefore in goat ruminal artery, curcumin at least in part, act via direct activation of sGC mediated cGMP pathway followed by opening of K(+) ion channel. However other mechanisms may not be ruled out.
authors.affiliation: Department of Pharmacology and Toxicology, College of Veterinary Science and Animal Husbandry, Orissa University of Agriculture and Technology, Orissa 751003, India.
authors.forename: J R
authors.initials: JR
authors.lastname: Dash
authors.forename: S C
authors.initials: SC
authors.lastname: Parija
mesh_terms: D015761:4-Aminopyridine / Q000494:pharmacology; D000109:Acetylcholine / Q000494:pharmacology; D000818:Animals; D003474:Curcumin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000187:drug effects / Q000502:physiology*; D004789:Enzyme Activation / Q000187:drug effects; D006041:Goats / Q000033:anatomy & histology / Q000502:physiology*; D006162:Guanylate Cyclase / Q000235:genetics / Q000378:metabolism*; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D009599:Nitroprusside / Q000494:pharmacology; D010069:Oxadiazoles / Q000494:pharmacology; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism*; D012417:Rumen / Q000098:blood supply*; D012701:Serotonin / Q000494:pharmacology; D000071756:Soluble Guanylyl Cyclase; D046509:Tissue Culture Techniques; D014661:Vasoconstriction / Q000187:drug effects*
chemical_list: C116907:1H-(1,2,4)oxadiazolo(4,3-a)-quiloxalin-1-one; D010069:Oxadiazoles; D018160:Receptors, Cytoplasmic and Nuclear; D009599:Nitroprusside; D012701:Serotonin; D015761:4-Aminopyridine; D006162:Guanylate Cyclase; D000071756:Soluble Guanylyl Cyclase; D003474:Curcumin; D000109:Acetylcholine; D019331:NG-Nitroarginine Methyl Ester
keywords: 4-AP; Curcumin; Goat ruminal artery; L-NAME; ODQ; s-Guanylatecyclase