title: HSF2BP represses BNC1 transcriptional activity by sequestering BNC1 to the cytoplasm.
abstract: Basonuclin (BNC1), a zinc finger transcriptional factor, is essential for mouse spermatogenesis. However, the regulatory mechanisms of BNC1 in spermatogenesis are poorly understood. In this study, we identified HSF2BP, a testis-specific binding protein of HSF2, as a binding partner of BNC1 by using yeast two-hybrid screening. HSF2BP could interact with and inhibit BNC1 transcriptional activity without affecting its expression level. Moreover, coexpression of HSF2BP with BNC1 resulted in a striking redistribution of BNC1 to the cytoplasm. These data suggest that HSF2BP may play a pivotal role in regulating BNC1 transcriptional activity and subcellular localization during spermatogenesis.
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Yujian
authors.initials: Y
authors.lastname: Wu
authors.forename: Shangying
authors.initials: S
authors.lastname: Liao
authors.forename: Xiuxia
authors.initials: X
authors.lastname: Wang
authors.forename: Si
authors.initials: S
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Wang
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Han
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry / Q000502:physiology*; D003593:Cytoplasm / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D015870:Gene Expression; D005786:Gene Expression Regulation; D057809:HEK293 Cells; D006360:Heat-Shock Proteins; D006801:Humans; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D054730:Protein Interaction Domains and Motifs; D021381:Protein Transport; D013091:Spermatogenesis; D013737:Testis / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: C511595:Bnc1 protein, mouse; D002352:Carrier Proteins; D004268:DNA-Binding Proteins; C583217:HSF2BP protein, mouse; D006360:Heat-Shock Proteins; D014157:Transcription Factors