title: A combined fluorescence spectroscopy, confocal and 2-photon microscopy approach to re-evaluate the properties of sphingolipid domains.
abstract: The aim of this study is to provide further insight about the interplay between important signaling lipids and to characterize the properties of the lipid domains formed by those lipids in membranes containing distinct composition. To this end, we have used a combination of fluorescence spectroscopy, confocal and two-photon microscopy and a stepwise approach to re-evaluate the biophysical properties of sphingolipid domains, particularly lipid rafts and ceramide (Cer)-platforms. By using this strategy we were able to show that, in binary mixtures, sphingolipids (Cer and sphingomyelin, SM) form more tightly packed gel domains than those formed by phospholipids with similar acyl chain length. In more complex lipid mixtures, the interaction between the different lipids is intricate and is strongly dictated by the Cer-to-Chol ratio. The results show that in quaternary phospholipid/SM/Chol/Cer mixtures, Cer forms gel domains that become less packed as Chol is increased. Moreover, the extent of gel phase formation is strongly reduced in these mixtures, even though Cer molar fraction is increased. These results suggest that in biological membranes, lipid domains such as rafts and ceramide platforms, might display distinctive biophysical properties depending on the local lipid composition at the site of the membrane where they are formed, further highlighting the potential role of membrane biophysical properties as an underlying mechanism for mediating specific biological processes.
authors.affiliation: Centro de Química Física Molecular and Institute of Nanoscience and Nanotechnology, Instituto Superior Técnico, Universidade Técnica de Lisboa, Complexo I, Av. Rovisco Pais, 1049-001 Lisboa, Portugal.
authors.forename: Sandra N
authors.initials: SN
authors.lastname: Pinto
authors.forename: Fábio
authors.initials: F
authors.lastname: Fernandes
authors.forename: Alexander
authors.initials: A
authors.lastname: Fedorov
authors.forename: Anthony H
authors.initials: AH
authors.lastname: Futerman
authors.forename: Liana C
authors.initials: LC
authors.lastname: Silva
authors.forename: Manuel
authors.initials: M
authors.lastname: Prieto
mesh_terms: D002518:Ceramides / Q000737:chemistry*; D002784:Cholesterol / Q000737:chemistry*; D005456:Fluorescent Dyes; D008051:Lipid Bilayers / Q000737:chemistry*; D021962:Membrane Microdomains / Q000737:chemistry*; D036641:Microscopy, Fluorescence, Multiphoton; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013109:Sphingomyelins / Q000737:chemistry*; D013696:Temperature
chemical_list: D002518:Ceramides; D005456:Fluorescent Dyes; D008051:Lipid Bilayers; D010714:Phosphatidylethanolamines; D013109:Sphingomyelins; D002784:Cholesterol
keywords: 1,2-dioleoyl-sn-glycero-3-phosphocholine; 1,2-dipalmitoyl-sn-glycero-3-phosphocholine; 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(nitro-2-1,3-benzoxadiazol-4yl); 1,6-diphenyl-1,3,5-hexatriene; 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine; 2-photon microscopy; 6-dodecanoyl-2-(dimethylamino)naphthalene; Cer; Ceramide; Ceramide-platforms; Chol; Cholesterol; DOPC; DPH; DPPC; Fluorescence spectroscopy and microscopy; GUV; Giant unilamellar vesicles; Laurdan; Lipid rafts; Liquid disorder; Liquid ordered; MLV; Membrane lipid domains; Multilamellar vesicles; N-palmitoyl-D-erythro-sphingosine; N-palmitoyl-D-erythro-sphingosylphosphorylcholine; N-rhodamine-dipalmitoylphosphatidylethanolamine; NBD-DPPE; PCer; POPC; PSM; Rho-DOPE; SLs; SM; Sphingolipids; Sphingomyelin; l(d); l(o); t-PnA; trans-parinaric acid