title: The role of short-chain conjugated poly-(R)-3-hydroxybutyrate (cPHB) in protein folding.
abstract: Poly-(R)-3-hydroxybutyrate (PHB), a linear polymer of R-3-hydroxybutyrate (R-3HB), is a fundamental constituent of biological cells. Certain prokaryotes accumulate PHB of very high molecular weight (10,000 to >1,000,000 residues), which is segregated within granular deposits in the cytoplasm; however, all prokaryotes and all eukaryotes synthesize PHB of medium-chain length (~100-200 residues) which resides within lipid bilayers or lipid vesicles, and PHB of short-chain length (<12 residues) which is conjugated to proteins (cPHB), primarily proteins in membranes and organelles. The physical properties of cPHB indicate it plays important roles in the targeting and folding of cPHB-proteins. Here we review the occurrence, physical properties and molecular characteristics of cPHB, and discuss its influence on the folding and structure of outer membrane protein A (OmpA) of Escherichia coli.
authors.affiliation: Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA. rnreusch@msu.edu.
authors.forename: Rosetta N
authors.initials: RN
authors.lastname: Reusch
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D006885:Hydroxybutyrates / Q000737:chemistry / Q000378:metabolism*; D011091:Polyesters / Q000737:chemistry / Q000378:metabolism*; D017510:Protein Folding; D000094122:Polyhydroxybutyrates
chemical_list: D001425:Bacterial Outer Membrane Proteins; D006885:Hydroxybutyrates; D011091:Polyesters; C078167:OMPA outer membrane proteins; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates