title: Flash photolysis and pulse radiolysis studies on elastin hydrolysates.
abstract: The formation of reactive species and free radicals in water soluble elastin hydrolysates have been investigated by pulse radiolysis and flash photolysis. Elastin hydrolysates were obtained by hydrolysis of elastin extracted from aorta. An investigation of the photochemical properties of elastin hydrolysates in water was carried out using nanosecond laser irradiation. The transient spectra of elastin hydrolysates solution excited at 266 nm showed two bands. One of them with maximum at 295 nm and the second one with maximum at 400 nm. The reactions of hydrated electrons and ˙OH radicals with elastin have been studied by pulse radiolysis. In the absorption spectra of products resulting from the reaction of elastin with e(aq)(-) small maximum absorption in UV and visible light was observed. In the absorption spectra of products resulting from the reaction of the hydroxyl radicals with elastin two bands were observed. The first one at 320 nm and the second one at 410 nm. Reaction of OH radicals with elastin hydrolysates lead to formation of Tyr phenoxyl radicals with absorption at 410 nm. The influence of the addition of sodium azide NaN3 on the formation of the transients was evaluated.
authors.affiliation: Faculty of Chemistry, Nicolaus Copernicus University, Torun, Poland. as@chem.uni.torun.pl
authors.forename: Alina
authors.initials: A
authors.lastname: Sionkowska
mesh_terms: D004549:Elastin / Q000737:chemistry / Q000528:radiation effects*; D005609:Free Radicals / Q000737:chemistry; D007834:Lasers; D010315:Particle Accelerators; D010782:Photolysis; D011492:Protein Hydrolysates / Q000737:chemistry / Q000528:radiation effects*; D011675:Pulse Radiolysis
chemical_list: D005609:Free Radicals; D011492:Protein Hydrolysates; D004549:Elastin
keywords: Elastin hydrolysates; Flash photolysis; Photochemistry; Pulse radiolysis; Transient spectra; UV irradiation