title: [HPLC specific chromatogram of Dendrobium officinale].
abstract: OBJECTIVE
  To establish the method of specific chromatogram analysis of ether extract of Dendrobium officinale for identification of D. officinale.
  
  
  METHODS
  Chromatographic separation was carried out at 30 degrees C on an Ultimate C18 column (4.6 mm x 250 mm, 5 microm) eluted with methanol and water containing 0.2% phosphoric acid in a gradient elution at a flow rate of 1.0 mL x min(-1). The detection wavelength was set at 280 nm. The similarity evaluation system for chromatographic fingerprint of NPC (National Pharmacopoeia Committee) was adopted to specific chromatogram construction.
  
  
  RESULTS
  The HPLC specific chromatogram of D. officinale was constructed with 6 common specific chromatographic peaks including naringenin as a reference peak.
  
  
  CONCLUSIONS
  The method shows good precision and repeatability of relative retention time. It can be used to identify D. officinale.
authors.affiliation: Institute of Materia Medica, Zhejiang Chinese Medical University, Hangzhou 310053, China.
authors.forename: Mei-Qiu
authors.initials: MQ
authors.lastname: Yan
authors.forename: Su-Hong
authors.initials: SH
authors.lastname: Chen
authors.forename: Gui-Yuan
authors.initials: GY
authors.lastname: Lv
authors.forename: Gui-Fen
authors.initials: GF
authors.lastname: Zhou
authors.forename: Xia
authors.initials: X
authors.lastname: Liu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D031667:Dendrobium / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D004987:Ethers / Q000737:chemistry; D015203:Reproducibility of Results
chemical_list: D004365:Drugs, Chinese Herbal; D004987:Ethers