title: High SKIP expression is correlated with poor prognosis and cell proliferation of hepatocellular carcinoma.
abstract: Ski-interacting protein (SKIP) is a transcriptional cofactor distinct from other cofactors and involved in the regulation of many cancer-related proteins. This study investigated the expression of SKIP and its potential clinical and biological significances in hepatocellular carcinoma (HCC). Immunohistochemistry and Western blot were performed to detect the expression of SKIP in clinical HCC samples and adjacent noncancerous tissues. In addition, expression of SKIP was correlated with clinicopathological variables, and univariate and multivariate survival analyses were performed to determine the prognostic significance. Moreover, the biological significance of the aberrant expression of SKIP was investigated in vitro. High SKIP expression was detected in clinical HCC samples compared with adjacent noncancerous tissues. Expression of SKIP correlated directly with the histological grades of HCC and high expression of SKIP was associated with a poor prognosis. SKIP depletion by small interfering RNA inhibited cell proliferation and blocked S phase entry in HepG2 cells. Owing to overexpression of SKIP in HCC tissues and its important role in predicting poor prognosis and the development of HCC, SKIP could be a potential prognostic marker and therapeutic target of HCC.
authors.affiliation: Department of Digestion, Affiliated Hospital of Nantong University, Medical College of Nantong University, Nantong 226001, Jiangsu, People's Republic of China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Liu
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Huang
authors.forename: Xiaopeng
authors.initials: X
authors.lastname: Cui
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.forename: Lixian
authors.initials: L
authors.lastname: Wei
authors.forename: Runzhou
authors.initials: R
authors.lastname: Ni
authors.forename: Cuihua
authors.initials: C
authors.lastname: Lu
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism; D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D059447:Cell Cycle Checkpoints / Q000235:genetics; D049109:Cell Proliferation; D005260:Female; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D016196:S Phase / Q000235:genetics; D016019:Survival Analysis; D055815:Young Adult
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D014408:Biomarkers, Tumor; C466999:SPHKAP protein, human