title: Sequential multi-molecule delivery using vortex-assisted electroporation.
abstract: We developed an on-chip microscale electroporation system that enables sequential delivery of multiple molecules with precise and independent dosage controllability into pre-selected identical populations of target cells. The ability to trap cells with uniform size distribution contributed to enhanced molecular delivery efficiency and cell viability. Additionally, the system provides real-time monitoring ability of the entire delivery process, allowing timely and independent modification of cell- and molecule-specific electroporation parameters. The precisely controlled amount of inherently membrane-impermeant molecules was transferred into human cancer cells by varying electric field strengths and molecule injection durations. The proposed microfluidic electroporation system's improved viability and comparable gene transfection efficiency to that of commercial systems suggest that the current system has great potential to expand the research fields that on-chip electroporation techniques can be used in.
authors.affiliation: The Rowland Institute at Harvard University, 100 Edwin H. Land Boulevard, Cambridge, MA 02142, USA.
authors.forename: Hoyoung
authors.initials: H
authors.lastname: Yun
authors.forename: Soojung Claire
authors.initials: SC
authors.lastname: Hur
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival; D053672:Electrochemotherapy / Q000295:instrumentation / Q000379:methods; D018274:Electroporation / Q000295:instrumentation* / Q000379:methods*; D004867:Equipment Design; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics; D046210:Microfluidic Analytical Techniques; D008856:Microscopy, Fluorescence; D010957:Plasmids; D014162:Transfection; D000097573:Red Fluorescent Protein
chemical_list: D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein; C485184:enhanced green fluorescent protein