title: Crystallization and preliminary X-ray study of a thermostable alanine racemase from Thermoanaerobacter tengcongensis MB4.
abstract: Alanine racemase (Alr(MB4)), a dimeric PLP-dependent thermostable enzyme from the anaerobic eubacterium Thermoanaerobacter tengcongensis MB4, was expressed and purified with a His(6) tag in a form suitable for X-ray crystallographic analysis. Crystals were grown by the hanging-drop vapour-diffusion method at 289 K using a solution consisting of 0.1 M bis-tris pH 7.0, 22%(w/v) polyethylene glycol 4000. X-ray diffraction data were collected to 2.6 Å resolution. The crystal belonged to the orthorhombic space group P2(1)2(1)2(1), with two protein molecules in an asymmetric unit.
authors.affiliation: Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin 300457, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Dong
authors.forename: Shujing
authors.initials: S
authors.lastname: Xu
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: Lu
authors.forename: Guangzheng
authors.initials: G
authors.lastname: He
authors.forename: Ranran
authors.initials: R
authors.lastname: Zhao
authors.forename: Shuai
authors.initials: S
authors.lastname: Chen
authors.forename: Sheng
authors.initials: S
authors.lastname: Fu
authors.forename: Jiansong
authors.initials: J
authors.lastname: Ju
mesh_terms: D000411:Alanine Racemase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D008969:Molecular Sequence Data; D046968:Thermoanaerobacter / Q000201:enzymology*
chemical_list: D001426:Bacterial Proteins; D000411:Alanine Racemase
keywords: Thermoanaerobacter tengcongensis; alanine racemase