title: Upregulation of the TPX2 gene is associated with enhanced tumor malignance of esophageal squamous cell carcinoma.
abstract: OBJECTIVE
  To explore the expression of TPX2 and its significance in esophageal squamous cell carcinoma (ESCC) tissue and approach relationship between the TPX2 and clinicopathological characteristic of esophageal squamous cell carcinoma.
  
  
  METHODS
  RT-PCR and immunohistochemical staining were used to compare the expression of TPX2 in 62 esophageal squamous cell carcinoma, 31 atypical hyperplasia and 62 normal esophageal mucosa.
  
  
  RESULTS
  In ESCC, atypical hyperplasia and in normal mucous membrane tissues, the positive rate of TPX2 protein expression was 85.5% (53/62), 51.6% (16/31) and 4.8% (3/62); the positive rate of TPX2 mRNA expression was 65.5% (40/62), 35.5 (11/31) and 4.83% (3/62). The expression of TPX2 protein and mRNA were correlated with invasive depth and lymphatic metastasis of ESCC (P<0.01).
  
  
  CONCLUSIONS
  Overexpression of TPX2 may be risk factor of lymph node in esophageal carcinoma, and maybe a potential biomarker for early diagnosis and prognosis of esophageal squamous cell carcinoma.
authors.affiliation: Department of Medical Laboratory, The First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China. Electronic address: xingyunerliu@163.com.
authors.forename: Hong-Chun
authors.initials: HC
authors.lastname: Liu
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xli-Lin
authors.initials: XL
authors.lastname: Wang
authors.forename: Wang-Sen
authors.initials: WS
authors.lastname: Qin
authors.forename: Yu-Han
authors.initials: YH
authors.lastname: Liu
authors.forename: Lan
authors.initials: L
authors.lastname: Zhang
authors.forename: Chang-Lian
authors.initials: CL
authors.lastname: Zhu
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D018797:Cell Cycle Proteins / Q000096:biosynthesis / Q000235:genetics*; D004938:Esophageal Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology; D004947:Esophagus / Q000473:pathology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D006965:Hyperplasia; D008207:Lymphatic Metastasis; D008297:Male; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis / Q000235:genetics*; D008875:Middle Aged; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness; D009687:Nuclear Proteins / Q000096:biosynthesis / Q000235:genetics*; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D015854:Up-Regulation
chemical_list: D018797:Cell Cycle Proteins; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; D012333:RNA, Messenger; C122167:TPX2 protein, human
keywords: Esophageal squamous cell carcinoma; Immunohistochemical; RT-PCR; TPX2