title: Effect of pretreatment with omega-3 polyunsaturated fatty acids (PUfas) on hematological parameters and platelets aggregation in patients during elective coronary artery bypass grafting.
abstract: OBJECTIVE
  [corrected] Using omega-3 polyunsaturated fatty acids (PUFAs) in coronary artery bypass graft surgery (CABG) could provide protection against ischemic-reperfusion damage, prevention of postoperative arrhyth mia and attenuation of inflammatory response. However, omega-3 PUFAs inhibit cyclooxygenase (and thus decrease the synthesis of thromboxane A2 from arachidonic acid in platelets), which leads to decreased platelet aggregation. In cardiac surgery it is necessary to achieve a balance between inhibition and full platelets function. It is as well as im portant to closely follow hematological parameters, im paired by CABG itself. Therefore, the aim of the study was to establish the effects of pretreatment with omega-PUFAs on hematological parameters and plateletes aggre gation in patients with elective CABG.
  
  
  METHODS
  This prospective, randomized, placebo-controlled, single-center trial was performed on parallel groups. The patients (n = 40) undergoing elective CABG were randomized receivin preoperative intravenous omega-3 PUFAs (Omegaven 10%) infusion (the PUFAs group) or the same volume of 0.9% saline solution infusion (the control group). Infusion was given a day before surgery and repeated four hours before starting extracorporeal circulation (CPB) via the pe ripheral vein at single doses of 100 mL (25 mL/h). Platelet function analysis was performed using multiple electrode aggregometry (MEA, multiplate-analyzer) before starting CPB and 2 h postoperatively for the patients of both groups. Results. There were no clinically relevant differ ences in baseline characteristics between the groups. He matological parameters were not significantly different between the groups pre-, intra- and postoperatively. Dur ing the first 24 h after surgery, the loss of blood was simi lar in the PUFAs and the control group (680 +/- 274 mL and 608 +/- 210 mL, respectively; p = 0.356). Postopera tively, platelet aggregation was not significantly different between the PUFAs and the control group in adenosine diphosphate (ADP) test (39 +/- 11 and 42 +/- 15, respec tively; p = 0.701), arachidonic acid (ASPI) test (64 +/- 24 and 70 +/- 27, respectively; p = 0.525) and trombin receptor-activating peptide (TRAP) test (68 +/- 25 and 75 + 26, respectively; p = 0.396), while their aggregation in collagen (COL) test was statistically significantly lower in the PUFAs related to the control group (32 +/- 15 and 47 +/- 20, re spectively; p = 0.009). Conclusion. Acute pretreatment with omega-3 PUFAs insignificantly affected the activity of platelets and did not influence postoperative blood loss.
authors.affiliation: Clinic of Anesthesiology and Intensive Care, Military Medical Academy, Belgrade, Serbia.
authors.forename: Milić
authors.initials: M
authors.lastname: Veljović
authors.forename: Ivana
authors.initials: I
authors.lastname: Mihajlović
authors.forename: Vesna
authors.initials: V
authors.lastname: Subota
authors.forename: Mirjana
authors.initials: M
authors.lastname: Antunović
authors.forename: Jasna
authors.initials: J
authors.lastname: Jevdjić
authors.forename: Ivo
authors.initials: I
authors.lastname: Udovicić
authors.forename: Ana
authors.initials: A
authors.lastname: Popadić
authors.forename: Tatjana
authors.initials: T
authors.lastname: Vulović
mesh_terms: D000368:Aged; D016063:Blood Loss, Surgical / Q000517:prevention & control; D001026:Coronary Artery Bypass; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010974:Platelet Aggregation / Q000187:drug effects*; D011300:Preoperative Care
chemical_list: D015525:Fatty Acids, Omega-3