title: Amikacin-induced Fin Reduction is Mediated by Autophagy.
abstract: Despite its medical use, little is known about the mechanisms underlying amikacin-induced embryotoxicity, including fin reduction, in zebrafish. In this study, we examined the expression of well-known autophagy markers mTOR (target of rapamycin), atg10 (autophagy-related gene), atg12 and LC3 (mammalian homolog of Atg8) in amikacin-treated zebrafish embryos. Our results indicated that the mRNA expression level of atg12 in the amikacin-treated group was significantly increased by 1.5-fold (p<0.05) compared with the corresponding mock control group, while the expression levels of atg10 and mTOR were significantly decreased by 0.74-fold (p<0.05) and 0.58-fold (p<0.05), respectively. Western blot analysis revealed that LC3 protein expression was induced by amikacin. Taken together, these data suggest that amikacin-induced fin reduction is mediated by fin cell autophagy.
authors.affiliation: Department of Chemistry, Tamkang University, 151, Yingzhuan Road, Danshui Dist., New Taipei City 25137, Taiwan, ROC.
authors.forename: I-Ting
authors.initials: IT
authors.lastname: Tsai
authors.forename: Ying-Hsin
authors.initials: YH
authors.lastname: Chen
authors.forename: Yau-Hung
authors.initials: YH
authors.lastname: Chen
authors.forename: Yun-Hsin
authors.initials: YH
authors.lastname: Wang
keywords: amikacin; autophagy; embryotoxicity; fin reduction; zebrafish