title: [TSPO ligand PK11195 and MAPK inhibitor UO126 modulate tspo expression level].
abstract: Translocator protein TSPO and MAPK signaling partway are important regulators of numerous cell functions including carcinogenesis, cell proliferation and apoptosis. We have studied MAPK inhibitor UO126 and TSPO specific ligand PK11195 effects on TSPO expression level in melanoma cells. Using immunocytochemical staining, we have detected increased expression of TSPO after incubation with PK11195 and UO126 in all concentrations. These results were confirmed by real time PCR: the increase in mRNA TSPO expression level was detected after incubation with PK11195 in concentration 100 nmol/L, and with UO126 in concentration 10 micromol/L. In the case of a combination of treatments with PK11195 and UO126, we also observed an increase in the level of TSPO expression. So, we conclus de that TSPO ligand PK11195 and MAPK signaling partway inhibitor UO126 modulate TSPO expression. These data are crucial for indentifying of regulatory processes for TSPO expression. Pathogenetic interconnection between MAPK signaling partway and TSPO is important for novel therapeutic approaches in melanoma treatment.
authors.forename: S N
authors.initials: SN
authors.lastname: Gyrylova
authors.forename: T G
authors.initials: TG
authors.lastname: Ruksha
authors.forename: A V
authors.initials: AV
authors.lastname: Komina
mesh_terms: D002070:Butadienes / Q000494:pharmacology; D045744:Cell Line, Tumor; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D007546:Isoquinolines / Q000494:pharmacology; D020935:MAP Kinase Signaling System / Q000235:genetics; D008545:Melanoma / Q000235:genetics / Q000378:metabolism; D020929:Mitogen-Activated Protein Kinase Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009570:Nitriles / Q000494:pharmacology; D018079:Receptors, GABA / Q000235:genetics / Q000378:metabolism
chemical_list: D002070:Butadienes; D007546:Isoquinolines; D009570:Nitriles; D018079:Receptors, GABA; C499229:TSPO protein, human; C113580:U 0126; D020929:Mitogen-Activated Protein Kinase Kinases; C037850:PK 11195