title: An ultrasonic method for separation of epiphytic microbes from freshwater submerged macrophytes.
abstract: Epiphytic microbes are common inhabitants of freshwater submerged macrophytes, which play an important role in aquatic ecosystems. An important precondition for studying the epiphytic microbes is having an effective method of separating the attached microbes from the host macrophytes. We developed an ultrasound-based method for separating epiphytic microbes from freshwater submerged macrophytes, optimized the conditions of ultrasonic separation with an orthogonal experimental design, and compared the optimized ultrasonic method with manual separation. This method can be particularly useful for freshwater submerged macrophytes having a complex morphology.
authors.affiliation: School of Environmental Science and Public Health, Wenzhou Medical University, Wenzhou, China; State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing, China.
authors.forename: Xianlei
authors.initials: X
authors.lastname: Cai
authors.forename: Guang
authors.initials: G
authors.lastname: Gao
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Xiangming
authors.initials: X
authors.lastname: Tang
authors.forename: Jiangyu
authors.initials: J
authors.lastname: Dai
authors.forename: Dan
authors.initials: D
authors.lastname: Chen
authors.forename: Yuzhi
authors.initials: Y
authors.lastname: Song
mesh_terms: D059001:Aquatic Organisms; D001419:Bacteria / Q000302:isolation & purification*; D058491:Bacterial Load; D002452:Cell Count; D000460:Chlorophyta / Q000166:cytology*; D017753:Ecosystem; D005163:Factor Analysis, Statistical; D005618:Fresh Water / Q000382:microbiology; D031321:Hydrocharitaceae / Q000382:microbiology*; D018520:Plant Shoots / Q000382:microbiology*; D031821:Potamogetonaceae / Q000382:microbiology*; D013010:Sonication / Q000379:methods*; D013559:Symbiosis
keywords: Epiphytic microbes; Submerged macrophytes; Ultrasonic separation