title: A multi-parametric microarray for protein profiling: simultaneous analysis of 8 different cytochromes via differentially element tagged antibodies and laser ablation ICP-MS.
abstract: The paper presents a new multi-parametric protein microarray embracing the multi-analyte capabilities of laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The combination of high throughput reverse phase protein microarrays with element tagged antibodies and LA-ICP-MS makes it possible to detect and quantify many proteins or biomarkers in multiple samples simultaneously. A proof of concept experiment is performed for the analysis of cytochromes particularly of cytochrome P450 enzymes, which play an important role in the metabolism of xenobiotics such as toxicants and drugs. With the aid of the LA-ICP-MS based multi-parametric reverse phase protein microarray it was possible to analyse 8 cytochromes in 14 different proteomes in one run. The methodology shows excellent detection limits in the lower amol range and a very good linearity of R(2) ≥ 0.9996 which is a prerequisite for the development of further quantification strategies.
authors.affiliation: BAM Federal Institute for Materials Research and Testing, Richard-Willstaetter-Straße 11, 12489 Berlin, Germany. larissa.waentig@bam.de.
authors.forename: Larissa
authors.initials: L
authors.lastname: Waentig
authors.forename: Sandra
authors.initials: S
authors.lastname: Techritz
authors.forename: Norbert
authors.initials: N
authors.lastname: Jakubowski
authors.forename: Peter H
authors.initials: PH
authors.lastname: Roos
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D003580:Cytochromes / Q000032:analysis*; D005260:Female; D053685:Laser Therapy / Q000379:methods*; D008297:Male; D008862:Microsomes, Liver / Q000737:chemistry; D040081:Protein Array Analysis / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013054:Spectrophotometry, Atomic / Q000379:methods*
chemical_list: D000906:Antibodies; D003580:Cytochromes