title: Metal ion leachates and the physico-chemical stability of biotherapeutic drug products.
abstract: Metal ions that leach into biotherapeutic drug product solution during manufacturing and storage, result in contamination that can cause physico-chemical degradation of the active molecule. In this review, we describe various mechanisms by which metal ion leachates can interact with therapeutic proteins and antibodies. Site-specific modifications due to metal catalyzed oxidation (MCO) of the therapeutic proteins cause them to become destabilized and potentially increasingly aggregation prone. We have examined the molecular sequences and structures for three case studies, human relaxin (hRlx), human growth hormone (hGH) and an IgG2 mAb to rationalize the experimental findings related to their MCO. The analysis indicates that metal-binding sites lie in close spatial proximities to predicted aggregation prone regions in these molecules. From the perspective of pharmaceutical development of biotherapeutic drugs, this link between molecular origins of MCO and subsequent aggregation is undesirable. This article further suggests molecular design strategies involving disruption of APRs that may also help mitigate the impact of metal ion leachates on biotherapeutic drug products as well as improving their solubility.
authors.forename: Sandeep
authors.initials: S
authors.lastname: Kumar
authors.forename: Shuxia
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Pfizer Inc., AA4, 700 Chesterfield Parkway West, Chesterfield, MO 63017, USA. satish.singh@pfizer.com.
authors.forename: Satish K
authors.initials: SK
authors.lastname: Singh
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D001702:Biopharmaceutics / Q000592:standards; D055598:Chemical Phenomena; D004340:Drug Contamination / Q000517:prevention & control; D004355:Drug Stability; D019382:Human Growth Hormone / Q000737:chemistry*; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry*; D008670:Metals / Q000737:chemistry*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D055550:Protein Stability; D012065:Relaxin / Q000737:chemistry*
chemical_list: D007074:Immunoglobulin G; D008670:Metals; D019382:Human Growth Hormone; D012065:Relaxin