title: Diverse neuronal lineages make stereotyped contributions to the Drosophila locomotor control center, the central complex.
abstract: The Drosophila central brain develops from a fixed number of neuroblasts. Each neuroblast makes a clone of neurons that exhibit common trajectories. Here we identified 15 distinct clones that carry larval-born neurons innervating the Drosophila central complex (CX), which consists of four midline structures including the protocerebral bridge (PB), fan-shaped body (FB), ellipsoid body (EB), and noduli (NO). Clonal analysis revealed that the small-field CX neurons, which establish intricate projections across different CX substructures, exist in four isomorphic groups that respectively derive from four complex posterior asense-negative lineages. In terms of the region-characteristic large-field CX neurons, we found that two lineages make PB neurons, 10 lineages produce FB neurons, three lineages generate EB neurons, and two lineages yield NO neurons. The diverse FB developmental origins reflect the discrete input pathways for different FB subcompartments. Clonal analysis enlightens both development and anatomy of the insect locomotor control center.
authors.affiliation: Howard Hughes Medical Institute, Janelia Farm Research Campus, 19700 Helix Drive, Ashburn, VA, USA.
authors.forename: Jacob S
authors.initials: JS
authors.lastname: Yang
authors.affiliation: Howard Hughes Medical Institute, Janelia Farm Research Campus, 19700 Helix Drive, Ashburn, VA, USA.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Awasaki
authors.affiliation: Howard Hughes Medical Institute, Janelia Farm Research Campus, 19700 Helix Drive, Ashburn, VA, USA.
authors.forename: Hung-Hsiang
authors.initials: HH
authors.lastname: Yu
authors.affiliation: Department of Neurobiology, University of Massachusetts, 364 Plantation Street, Worcester, MA, USA.
authors.forename: Yisheng
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Neurobiology, University of Massachusetts, 364 Plantation Street, Worcester, MA, USA.
authors.forename: Peng
authors.initials: P
authors.lastname: Ding
authors.affiliation: Department of Neurobiology, University of Massachusetts, 364 Plantation Street, Worcester, MA, USA.
authors.forename: Jui-Chun
authors.initials: JC
authors.lastname: Kao
authors.affiliation: Howard Hughes Medical Institute, Janelia Farm Research Campus, 19700 Helix Drive, Ashburn, VA, USA.|Department of Neurobiology, University of Massachusetts, 364 Plantation Street, Worcester, MA, USA.
authors.forename: Tzumin
authors.initials: T
authors.lastname: Lee
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001921:Brain / Q000166:cytology* / Q000254:growth & development*; D016827:CD8 Antigens / Q000378:metabolism; D019070:Cell Lineage / Q000502:physiology; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism; D007814:Larva; D008124:Locomotion / Q000502:physiology*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D009415:Nerve Net / Q000254:growth & development* / Q000378:metabolism; D058953:Neural Stem Cells / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000502:physiology*
chemical_list: C510146:BRP protein, Drosophila; D016827:CD8 Antigens; D029721:Drosophila Proteins; D008164:Luminescent Proteins