title: [Wuziyanzong compound relieves oxidative stress injury and inhibits the apoptosis of Sertoli cells].
abstract: OBJECTIVE
  To investigate the effects of Wuziyanzong Compound on oxidative stress injury and the apoptosis of Sertoli cells.
  
  
  METHODS
  We added H2O2 (100 mmol/L) and Wuziyanzong extract to the culture solution of TM4 cells, which were divided into a control, a model and three Wuziyanzong groups (low dose: 0.2 mg/ml, medium dose: 1 mg/ml, and high dose: 5 mg/ml). After 24 hours of intervention, we detected the absorbance value by MTT assay, measured the SOD activity and MDA content using the xanthine oxidase and thiobarbituric acid method respectively, and determined the apoptosis and survival rate of the cells by flow cytometry and the expression of caspase-3 mRNA by real time PCR.
  
  
  RESULTS
  After treated with low-, medium- and high-dose Wuziyanzong Compound, the absorbance value of the TM4 cells was increased from 0.23 +/- 0.01 in the model group to 0.27 +/- 0.02, 0.30 +/- 0.01 and 0.35 +/- 0.01, the SOD activity increased from (8.32 +/- 0.62) U/ml to (9.69 +/- 0.59), (10.47 +/- 0.74) and (12.28 +/- 0.38) U/ ml, the MDA content decreased from (6.99 +/- 0.74) mol/L to (5.78 +/- 0.28), (4.19 +/- 0.43) and (3.42 +/- 0.51) mol/L, the cell apoptosis rate decreased from (21.63 +/- 1.38)% to (17.87 +/- 0.75), (14.10 +/- 0.50) and (9.53 +/- 1.34)%, the cell survival rate increased from (65.67 +/- 7.48)% to (70.37 +/- 1.12), (72.57 +/- 1.95) and (81.60 +/- 2.46)%, and the relative expression of caspase-3 mRNA decreased from 2.69 +/- 0.16 to 2.47 +/- 0.12, 2.38 +/- 0.16 and 1.96 +/- 0.11, respectively.
  
  
  CONCLUSIONS
  Wuziyanzong Compound can relieve oxidative stress injury and inhibit the apoptosis of TM4 cells.
authors.affiliation: Department of Urology, Jiangyin Hospital Affiliated to Nantong University, Jiangyin, Jiangsu 214400, China. goldlong_y@163.com
authors.forename: Jin-Long
authors.initials: JL
authors.lastname: Yin
authors.forename: Yuan
authors.initials: Y
authors.lastname: Xu
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D012708:Sertoli Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; C528421:wuzi yanzong