title: Differences between men and women in the regulation of adipose 11β-HSD1 and in its association with adiposity and insulin resistance.
abstract: This study explored sex differences in 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) activity and gene expression in isolated adipocytes and adipose tissue (AT), obtained via subcutaneous biopsies from non-diabetic subjects [58 M, 64 F; age 48.3 ± 15.3 years, body mass index (BMI) 27.2 ± 3.9 kg/m²]. Relationships with adiposity and insulin resistance (IR) were addressed. Males exhibited higher 11β-HSD1 activity in adipocytes than females, but there was no such difference for AT. In both men and women, adipocyte 11β-HSD1 activity correlated positively with BMI, waist circumference, % body fat, adipocyte size and with serum glucose, triglycerides and low-density lipoprotein:high-density lipoprotein (LDL:HDL) ratio. Positive correlations with insulin, HOMA-IR and haemoglobin A1c (HbA1c) and a negative correlation with HDL-cholesterol were significant only in males. Conversely, 11β-HSD1 activity in AT correlated with several markers of IR and adiposity in females but not in males, but the opposite pattern was found with respect to 11β-HSD1 mRNA expression. This study suggests that there are sex differences in 11β-HSD1 regulation and in its associations with markers of obesity and IR.
authors.affiliation: AstraZeneca R&D, Macclesfield, UK.
authors.forename: J
authors.initials: J
authors.lastname: DeSchoolmeester
authors.forename: J
authors.initials: J
authors.lastname: Palming
authors.forename: T
authors.initials: T
authors.lastname: Persson
authors.forename: M J
authors.initials: MJ
authors.lastname: Pereira
authors.forename: E
authors.initials: E
authors.lastname: Wallerstedt
authors.forename: H
authors.initials: H
authors.lastname: Brown
authors.forename: D
authors.initials: D
authors.lastname: Gill
authors.forename: F
authors.initials: F
authors.lastname: Renström
authors.forename: M
authors.initials: M
authors.lastname: Lundgren
authors.forename: M K
authors.initials: MK
authors.lastname: Svensson
authors.forename: A
authors.initials: A
authors.lastname: Rees
authors.forename: J W
authors.initials: JW
authors.lastname: Eriksson
mesh_terms: D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1 / Q000235:genetics / Q000378:metabolism*; D050154:Adiposity; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D001706:Biopsy; D015992:Body Mass Index; D048429:Cell Size; D002478:Cells, Cultured; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D006949:Hyperlipidemias / Q000209:etiology; D007333:Insulin Resistance; D008297:Male; D024821:Metabolic Syndrome / Q000150:complications / Q000503:physiopathology; D008875:Middle Aged; D050177:Overweight / Q000150:complications / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D012333:RNA, Messenger / Q000378:metabolism; D012727:Sex Characteristics; D050151:Subcutaneous Fat / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology
chemical_list: D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1; D015415:Biomarkers; D006442:Glycated Hemoglobin; D012333:RNA, Messenger; C517652:hemoglobin A1c protein, human; C468386:HSD11B1 protein, human
keywords: 11β-hydroxysteroid dehydrogenase type 1; adipocytes; adipose tissue; obesity; sex