title: Assessment of a commercially available point-of-care assay for the measurement of bovine cardiac troponin I concentration.
abstract: OBJECTIVE
  To assess a commercially available point-of-care assay for measurement of bovine cardiac troponin I (cTnI) concentration in blood and plasma samples.
  
  
  METHODS
  Prepared bovine plasma standard samples with known concentrations (0 to 1.0 ng/mL) of cTnI and blood and plasma samples obtained from 28 healthy 2.5-month-old Holstein calves.
  
  
  METHODS
  Coefficients of variation were calculated for concentrations of cTnI in prepared standards determined with the point-of-care assay, and values were compared with the known concentrations. The cTnI concentrations in blood samples obtained from calves determined with the point-of-care assay were compared with cTnI concentrations in plasma samples obtained from those animals determined with a validated immunoassay.
  
  
  RESULTS
  The coefficients of variation of cTnI concentrations determined for prepared standards by use of the point-of-care assay were low (< 20%) for standards with cTnI concentrations ≥ 0.025 ng/mL. The blood cTnI concentrations determined with the point-of-care assay were not significantly different from the plasma cTnI concentrations determined with the validated immunoassay.
  
  
  CONCLUSIONS
  Results of this study indicated the point-of-care assay had high precision for determination of cTnI concentrations in most evaluated prepared bovine plasma standard samples. The point-of-care assay may be useful for determination of circulating concentrations of cTnI in cattle.
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA.
authors.forename: Brandon C
authors.initials: BC
authors.lastname: Fraser
authors.forename: David E
authors.initials: DE
authors.lastname: Anderson
authors.forename: Brad J
authors.initials: BJ
authors.lastname: White
authors.forename: Matt D
authors.initials: MD
authors.lastname: Miesner
authors.forename: Carrie
authors.initials: C
authors.lastname: Wheeler
authors.forename: David
authors.initials: D
authors.lastname: Amrine
authors.forename: Jeff
authors.initials: J
authors.lastname: Lakritz
authors.forename: Tom
authors.initials: T
authors.lastname: Overbay
mesh_terms: D000818:Animals; D002417:Cattle / Q000097:blood*; D007118:Immunoassay / Q000379:methods / Q000662:veterinary; D019095:Point-of-Care Systems / Q000592:standards*; D019210:Troponin I / Q000097:blood*
chemical_list: D019210:Troponin I