title: Enzymatic Transformation of Ginsenoside Rb1 by Lactobacillus pentosus Strain 6105 from Kimchi.
abstract: Ginsenoside (ginseng saponin), the principal component of ginseng, is responsible for the pharmacological and biological activities of ginseng. We isolated lactic acid bacteria from Kimchi using esculin agar, to produce β-glucosidase. We focused on the bio-transformation of ginsenoside. Phylogenetic analysis was performed by comparing the 16S rRNA sequences. We identified the strain as Lactobacillus (strain 6105). In order to determine the optimal conditions for enzyme activity, the crude enzyme was incubated with 1 mM ginsenoside Rb1 to catalyse the reaction. A carbon substrate, such as cellobiose, lactose, and sucrose, resulted in the highest yields of β-glucosidase activity. Biotransformations of ginsenoside Rb1 were analyzed using TLC and HPLC. Our results confirmed that the microbial enzyme of strain 6105 significantly transformed ginsenoside as follows: Rb1→gypenoside XVII, Rd→F2 into compound K. Our results indicate that this is the best possible way to obtain specific ginsenosides using microbial enzymes from 6105 culture.
authors.affiliation: Korean Ginseng Center for Most Valuable Products and Ginseng Genetic Resource Bank, Kyung Hee University, Suwon 449-701, Korea.
authors.forename: Se-Hwa
authors.initials: SH
authors.lastname: Kim
authors.forename: Jin-Woo
authors.initials: JW
authors.lastname: Min
authors.forename: Lin-Hu
authors.initials: LH
authors.lastname: Quan
authors.forename: Sungyoung
authors.initials: S
authors.lastname: Lee
authors.forename: Dong-Uk
authors.initials: DU
authors.lastname: Yang
authors.forename: Deok-Chun
authors.initials: DC
authors.lastname: Yang
keywords: Ginsenoside; Lactobacillus pentosus; Panax ginseng; Transformation