title: Proper indication of BRAF(V600E) mutation testing in fine-needle aspirates of thyroid nodules.
abstract: BACKGROUND
  The aim of this study was to evaluate the proper indication of adjunctive BRAF(V600E) mutation analysis at the time of ultrasound-guided fine-needle aspiration in the diagnosis of thyroid nodules.
  
  
  METHODS
  This study included 518 nodules in 479 patients who underwent ultrasound-guided fine-needle aspiration with BRAF(V600E) mutation. We calculated and compared the diagnostic performances of cytology and cytology with BRAF(V600E) mutation analysis to detect malignancy among thyroid nodules according to ultrasound features and size.
  
  
  RESULTS
  Sensitivity, negative predictive value, and accuracy of cytology with BRAF(V600E) mutation analysis were significantly higher than those of cytology alone in thyroid nodules with suspicious ultrasound features, regardless of size. Diagnostic performances did not show significant differences between cytology and cytology with BRAF(V600E) mutation analysis in nodules without any suspicious ultrasound features, regardless of size.
  
  
  CONCLUSIONS
  The BRAF(V600E) mutation analysis was a useful adjunctive diagnostic tool in the diagnosis of thyroid nodules with suspicious ultrasound features regardless of size.
authors.affiliation: Department of Radiology, Research Institute of Radiological Science, Yonsei University, College of Medicine, Seoul, Korea.
authors.forename: Jieun
authors.initials: J
authors.lastname: Koh
authors.forename: Jong Rak
authors.initials: JR
authors.lastname: Choi
authors.forename: Kyung Hwa
authors.initials: KH
authors.lastname: Han
authors.forename: Eun-Kyung
authors.initials: EK
authors.lastname: Kim
authors.forename: Jung Hyun
authors.initials: JH
authors.lastname: Yoon
authors.forename: Hee Jung
authors.initials: HJ
authors.lastname: Moon
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Kwak
mesh_terms: D000328:Adult; D061765:Endoscopic Ultrasound-Guided Fine Needle Aspiration; D006801:Humans; D008875:Middle Aged; D009154:Mutation; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D016606:Thyroid Nodule / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology*
chemical_list: C482119:BRAF protein, human; D048493:Proto-Oncogene Proteins B-raf