title: Incorporation of poly[(R)-3-hydroxybutyrate] into cationic copolymers based on poly(2-(dimethylamino)ethyl methacrylate) to improve gene delivery.
abstract: The synthesis and gene transfection efficiency of a series of novel amphiphilic triblock copolymers with two hydrophilic poly(2-(dimethylamino)ethyl methacrylate) (DMAEMA) blocks flanking a central hydrophobic poly[(R)-3-hydroxybutyrate] (PHB) block is reported. These copolymers have significantly lower toxicity compared to the polyethyleneimine (PEI) and PDMAEMA homopolymer. Unexpectedly, the incorporation of PHB significantly improves the gene transfection efficiency as compared to PEI or PDMAEMA homopolymers.
authors.affiliation: Institute of Materials Research and Engineering, A*STAR (Agency for Science, Technology and Research), 3 Research Link, Singapore 117602, Singapore.
authors.forename: Xian Jun
authors.initials: XJ
authors.lastname: Loh
authors.forename: Shi Jie
authors.initials: SJ
authors.lastname: Ong
authors.forename: Yin Ting
authors.initials: YT
authors.lastname: Tung
authors.forename: Hoi Teng
authors.initials: HT
authors.lastname: Choo
mesh_terms: D000818:Animals; D019556:COS Cells; D002460:Cell Line; D002522:Chlorocebus aethiops; D005822:Genetic Vectors / Q000235:genetics; D057809:HEK293 Cells; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D006885:Hydroxybutyrates / Q000737:chemistry*; D008689:Methacrylates / Q000737:chemistry*; D009757:Nylons / Q000737:chemistry*; D010957:Plasmids / Q000235:genetics; D011091:Polyesters / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D000091402:Prohibitins; D014162:Transfection / Q000379:methods*; D000094122:Polyhydroxybutyrates
chemical_list: D006885:Hydroxybutyrates; D008689:Methacrylates; D009757:Nylons; D011091:Polyesters; D011108:Polymers; D000091402:Prohibitins; C000717328:PHB protein, human; C407037:poly(2-(dimethylamino)ethyl methacrylate); C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates
keywords: biodegradable polymer; gene delivery formulation; poly(2-(dimethylamino)ethyl methacrylate); poly[(R)-3-hydroxybutyrate]; transfection efficiency