title: Targeted siRNA delivery by anti-HER2 antibody-modified nanoparticles of mPEG-chitosan diblock copolymer.
abstract: This study aims to determine the specificity of anti-human epidermal growth factor receptor antibody (anti-HER2) modified monomethoxy polyethylene glycol-chitosan (mPEG-CS) nanoparticles (anti-HER2/mPEG-CS NPs) in delivering small interfering RNA (siRNA) to the human epidermal growth factor receptor 2 (HER2) positive cancer cells. Physicochemical properties of the siRNA-loaded anti-HER2/mPEG-CS NPs (anti-HER2/mPEG-CS-siRNA NPs), including size, surface charge, siRNA encapsulation efficiency, and in vitro release profile of siRNA from NPs, were characterized by particle size and zeta potential analyzer, and ultraviolet-visible spectrophotometer. MTT assay was used to study the in vitro cytotoxicity of the NPs. Fluorescent microscope and flow cytometer analysis results showed that anti-HER2/mPEG-CS-siRNA NPs had much efficient delivery of siRNA than the siRNA alone, Lipofectamine-siRNA complexes and mPEG-CS-siRNA NPs. These results demonstrated that anti-HER2/mPEG-CS-siRNA NPs had great potential applications as a targeted strategy for siRNA delivery.
authors.affiliation: Department of Ultrasound Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200127, PR China.
authors.forename: Yiyi
authors.initials: Y
authors.lastname: Wang
authors.forename: Peifeng
authors.initials: P
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Du
authors.forename: Ying
authors.initials: Y
authors.lastname: Sun
authors.forename: Fenghua
authors.initials: F
authors.lastname: Li
authors.forename: Yourong
authors.initials: Y
authors.lastname: Duan
mesh_terms: D000906:Antibodies / Q000737:chemistry* / Q000276:immunology; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D034741:RNA, Small Interfering / Q000008:administration & dosage*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000276:immunology*
chemical_list: D000906:Antibodies; D048271:Chitosan; D004337:Drug Carriers; D011092:Polyethylene Glycols; D011108:Polymers; D034741:RNA, Small Interfering; D018719:Erb-b2 Receptor Tyrosine Kinases; C028210:monomethoxypolyethylene glycol