title: Detection of long and short DNA using nanopores with graphitic polyhedral edges.
abstract: Graphene is a unique material with a thickness as low as a single atom, high in-plane conductivity and a robust lattice that is self-supporting over large length scales. Schematically, graphene is an ideal solid-state material for tuning the properties of a nanopore because self-supported sheets, ranging from single to multiple atomic layers, can create pores with near-arbitrary dimensions which can provide exquisite control of the electric field drop within the pore. In this study, we characterize the drilling kinetics of nanopores using a thermionic electron source and various electron beam fluxes to minimize secondary hole formation. Once established, we investigated the use of multilayer graphene to create highly tailored nanostructures including nanopores with graphite polyhedral crystals formed around the nanopore edge. Finally, we report on the translocation of double stranded and single stranded DNA through such graphene pores and show that the single stranded DNA translocates much slower allowing detection of extremely short fragments (25 nucleotides in length). Our findings suggest that the kinetic and controllable properties of graphene nanopores under sculpting conditions can be used to further enhance the detection of DNA analytes.
authors.affiliation: Department of Chemical and Biological Engineering, Drexel University, Philadelphia, Pennsylvania 19104, USA.
authors.forename: Kevin J
authors.initials: KJ
authors.lastname: Freedman
authors.forename: Chi Won
authors.initials: CW
authors.lastname: Ahn
authors.forename: Min Jun
authors.initials: MJ
authors.lastname: Kim
mesh_terms: D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D004553:Electric Conductivity; D006108:Graphite / Q000737:chemistry*; D008958:Models, Molecular; D058608:Nanopores; D009690:Nucleic Acid Conformation; D013499:Surface Properties
chemical_list: D006108:Graphite; D004247:DNA