title: Steroid hormone profiles in prepubertal obese children before and after weight loss.
abstract: BACKGROUND
  Little information is available on the steroid hormone profiles in obese children and their changes after weight loss.
  
  
  OBJECTIVE
  We compared liquid chromatography-tandem mass spectrometry of serum steroid hormone profiles between obese and normal-weight children and studied the differential effects of weight loss on these hormones.
  
  
  METHODS
  This study was a cross-sectional comparison between obese and normal-weight children and a longitudinal 1-year follow-up study during lifestyle intervention in obese children.
  
  
  METHODS
  The setting of the study was primary care.
  
  
  METHODS
  Forty obese prepubertal (mean age 8.5 ± 2.1 years, 48% female, mean body mass index 24.8 ± 3.5 kg/m(2)) and 40 normal-weight children matched for gender, age, and pubertal stage.
  
  
  METHODS
  The study consisted of an outpatient 1-year intervention program based on exercise, behavior, and nutrition therapy.
  
  
  METHODS
  Progesterone, 17-hydroxyprogesterone, 11-deoxycorticosterone, corticosterone, aldosterone, 11-deoxycortisol, cortisol, cortisone, dehydroepiandrosterone sulfate (DHEAS), androstenedione, T, dihydrotestosterone, insulin resistance index of the homeostasis model assessment, and blood pressure were measured.
  
  
  RESULTS
  Prepubertal obese children showed significantly increased androgens (DHEAS, androstenedione, T), mineralocorticoid precursor corticosterone, and glucocorticoids (11-deoxycortisol, cortisol, cortisone) compared with normal-weight children. In contrast to 20 obese children without weight loss, the 20 obese children with substantial weight loss demonstrated a significant decrease of cortisol, cortisone, and corticosterone. Androstenedione and T decreased but DHEAS remained elevated. Changes of the homeostasis model assessment correlated significantly positively with changes of cortisol (r = 0.38) and cortisone (r = 0.43) in partial regression analyses adjusted to changes of weight status.
  
  
  CONCLUSIONS
  In obese prepubertal children, the increased androgens, mineralocorticoid precursors, and glucocorticoids were responsive to weight loss in contrast to DHEAS, suggesting that DHEAS does not seem to be regulated by changes in body mass index.
authors.affiliation: Department of Pediatric Endocrinology, Diabetes, and Nutrition Medicine, Vestische Hospital for Children and Adolescents Datteln, University of Witten/Herdecke, D-45711 Datteln, Germany. T.reinehr@kinderklinik-datteln.de
authors.forename: Thomas
authors.initials: T
authors.lastname: Reinehr
authors.forename: Alexandra
authors.initials: A
authors.lastname: Kulle
authors.forename: Barbara
authors.initials: B
authors.lastname: Wolters
authors.forename: Nina
authors.initials: N
authors.lastname: Lass
authors.forename: Maik
authors.initials: M
authors.lastname: Welzel
authors.forename: Felix
authors.initials: F
authors.lastname: Riepe
authors.forename: Paul-Martin
authors.initials: PM
authors.lastname: Holterhus
mesh_terms: D000305:Adrenal Cortex Hormones / Q000097:blood*; D000728:Androgens / Q000097:blood*; D015992:Body Mass Index; D002648:Child; D019314:Dehydroepiandrosterone Sulfate / Q000097:blood; D005260:Female; D005938:Glucocorticoids / Q000097:blood*; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D008137:Longitudinal Studies; D008297:Male; D009765:Obesity / Q000097:blood*; D015431:Weight Loss
chemical_list: D000305:Adrenal Cortex Hormones; D000728:Androgens; D005938:Glucocorticoids; D019314:Dehydroepiandrosterone Sulfate; D006854:Hydrocortisone