title: Protective effects of osthole against myocardial ischemia/reperfusion injury in rats.
abstract: Osthole, a bioactive simple coumarin derivative extracted from a number of medicinal plants, such as Cnidium monnieri and Angelica pubescens, has been shown to exert a variety of pharmacological activities and is considered to have potential therapeutic applications. In this study, we investigated the protective effects of osthole against myocardial ischemia/reperfusion (I/R) injury in rats. Male Sprague-Dawley rats were randomly assigned to 1 of 5 groups: the sham-oeprated control group (control), the vehicle group (vehicle), and 3 treatment groups, which were treated with osthole at the concentration of 1, 10 or 50 mg/kg (intraperitoneally), respectively, upon the initiation of myocardial ischemia. Treatment with osthole suppressed the formation of lipid peroxidation products, enhanced the capacities of antioxidant enzymes and inhibited the expression of inflammatory cytokines following myocardial I/R injury. Moreover, treatment with osthole reduced high-mobility group box protein 1 (HMGB1) and phosphorylated nuclear factor (NF)-κB expression in ischemic myocardial tissue. These results demonstrate the protective effects of osthole against myocardial I/R injury in rats and suggest that these effects may be associated with its antioxidant and anti-inflammatory activities.
authors.affiliation: Department of Cardiovascular Surgery, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, Guangdong 510010, P.R. China.
authors.forename: Xian-Yue
authors.initials: XY
authors.lastname: Wang
authors.forename: Wen-Peng
authors.initials: WP
authors.lastname: Dong
authors.forename: Sheng-Hui
authors.initials: SH
authors.lastname: Bi
authors.forename: Zhi-Guo
authors.initials: ZG
authors.lastname: Pan
authors.forename: Hao
authors.initials: H
authors.lastname: Yu
authors.forename: Xiao-Wu
authors.initials: XW
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Ma
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Wei-Da
authors.initials: WD
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002121:Calcium Channel Blockers / Q000008:administration & dosage / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D003374:Coumarins / Q000008:administration & dosage / Q000494:pharmacology*; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D004789:Enzyme Activation / Q000187:drug effects; D024243:HMGB1 Protein / Q000378:metabolism; D006439:Hemodynamics; D018836:Inflammation Mediators / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000378:metabolism / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D016328:NF-kappa B / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D002121:Calcium Channel Blockers; D003374:Coumarins; D016207:Cytokines; D024243:HMGB1 Protein; D018836:Inflammation Mediators; D016328:NF-kappa B; D002374:Catalase; D013482:Superoxide Dismutase; C046627:osthol