title: Sequence selective recognition of double-stranded RNA at physiologically relevant conditions using PNA-peptide conjugates.
abstract: Conjugation of short peptide nucleic acids (PNA) with tetralysine peptides strongly enhanced triple helical binding to RNA at physiologically relevant conditions. The PNA hexamers and heptamers carrying cationic nucleobase and tetralysine modifications displayed high binding affinity for complementary double-stranded RNA without compromising sequence selectivity. The PNA-peptide conjugates had unique preference for binding double-stranded RNA, while having little, if any, affinity for double-stranded DNA. The cationic PNAs were efficiently taken up by HEK293 cells, whereas little uptake was observed for unmodified PNA.
authors.affiliation: Department of Chemistry, Binghamton University, The State University of New York, Binghamton, New York 13902, United States.
authors.forename: Oluwatoyosi
authors.initials: O
authors.lastname: Muse
authors.forename: Thomas
authors.initials: T
authors.lastname: Zengeya
authors.forename: Juddy
authors.initials: J
authors.lastname: Mwaura
authors.forename: Dziyana
authors.initials: D
authors.lastname: Hnedzko
authors.forename: Dennis W
authors.initials: DW
authors.lastname: McGee
authors.forename: Christof T
authors.initials: CT
authors.lastname: Grewer
authors.forename: Eriks
authors.initials: E
authors.lastname: Rozners
mesh_terms: D001483:Base Sequence; D001665:Binding Sites; D057809:HEK293 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D018613:Microscopy, Confocal; D015394:Molecular Structure; D020135:Peptide Nucleic Acids / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012330:RNA, Double-Stranded / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D020135:Peptide Nucleic Acids; D012330:RNA, Double-Stranded