title: Biotransformation of L-ornithine from L-arginine using whole-cell recombinant arginase.
abstract: A recombinant arginase was generated for a whole-cell biotransformation system to convert L-arginine to L-ornithine in Escherichia coli. The gene ARG1 coding arginase from Bos taurus liver was synthesized and expressed in E. coli BL21 (DE3) via pETDuet-1. The recombinant arginase was used to catalyze L-arginine to L-ornithine and urea. The reaction was optimal at pH 9.5 and 37 °C. Manganese (10(-5 )M) and Emulsifier OP-10 [0.033 % (v/v)] could promote arginase activity. In a scale up study, L-arginine conversion rate reached 98 % with a final concentration of 111.52 g L-ornithine/l.
authors.affiliation: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing, 210093, China, 328784567@qq.com.
authors.forename: Yueping
authors.initials: Y
authors.lastname: Zhan
authors.forename: Junzhong
authors.initials: J
authors.lastname: Liu
authors.forename: Pingting
authors.initials: P
authors.lastname: Mao
authors.forename: Hongjuan
authors.initials: H
authors.lastname: Zhang
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.forename: Qingcai
authors.initials: Q
authors.lastname: Jiao
mesh_terms: D000818:Animals; D001119:Arginase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D001120:Arginine / Q000737:chemistry / Q000378:metabolism*; D055162:Biocatalysis; D001711:Biotransformation; D002417:Cattle; D002617:Chemical Industry; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D005285:Fermentation; D009952:Ornithine / Q000737:chemistry / Q000378:metabolism*; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D014508:Urea / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D011994:Recombinant Proteins; D014508:Urea; D001120:Arginine; D009952:Ornithine; D001119:Arginase