title: A comparison of stromal cell-derived factor-1 expression during distraction osteogenesis and bone fracture in the mandible.
abstract: Distraction osteogenesis (DO) has been a widely applied technique in orthopedics and craniofacial surgery. However, the exact molecular mechanism by which the mechanical stimulus is translated into biological signals is still poorly understood. In this study, we examined and compared the expression of stromal cell-derived factor-1 (SDF-1) during mandibular distraction osteogenesis and fracture in rats, respectively. Forty-eight male Sprague-Dawley rats were divided into 2 groups and received unilateral distraction osteogenesis and rigid internal fixation, respectively, after the osteotomy on the right mandible. The harvested mandibles were examined radiographically, histologically, and immunohistochemically. We found that the expression of SDF-1 was mainly detected in the osteoblasts and blood vessels, and there were more intensive expression of SDF-1 in DO zones than in bone fracture zones. The quantitative analysis by enzyme-linked immunosorbent assay showed that SDF-1 reached a greater peak and maintained a longer period of up-regulation in DO than in fracture healing (P < 0.05). These results suggest that the distraction procedure markedly promotes the high expression of SDF-1 which facilitates the induction of bone formation during DO.
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University, Xi'an, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Cao
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Zhao-jie
authors.initials: ZJ
authors.lastname: Du
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.forename: Ya-bo
authors.initials: YB
authors.lastname: Zhang
authors.forename: Jian-fu
authors.initials: JF
authors.lastname: Sui
authors.forename: Yan-pu
authors.initials: YP
authors.lastname: Liu
authors.forename: De-lin
authors.initials: DL
authors.lastname: Lei
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D054377:Chemokine CXCL12 / Q000378:metabolism*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D017102:Fracture Healing / Q000502:physiology*; D008297:Male; D008337:Mandibular Fractures / Q000378:metabolism / Q000601:surgery; D010006:Osteoblasts / Q000378:metabolism; D019857:Osteogenesis, Distraction / Q000379:methods; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation
chemical_list: D054377:Chemokine CXCL12