title: A simple and rapid method to monitor the disassembly and reassembly of virus-like particles.
abstract: Protein fluorescence spectra (~300-440 nm) could be used as a simple and sensitive method to monitor the disassembly and reassembly of virus-like particles (VLPs). Insect cell expressed and purified HPV-16 L1 VLPs show significantly high fluorescence intensity, whereas the fluorescence is almost quenched after disassembly by adding the reducing agent. By removing the reducing agent, the fluorescence was restored to its original intensity, indicating the reassembly of VLPs. The data are consistent with enzyme-linked immunosorbent assay (ELISA) reactivity using conformation-specific mouse monoclonal antibody. The same method could be extended to VLPs of other viruses.
authors.affiliation: Research and Development Center, Indian Immunologicals, Rakshapuram, Gachibowli, Hyderabad 500032, India.
authors.forename: Burki
authors.initials: B
authors.lastname: Rajendar
authors.forename: Vuppu
authors.initials: V
authors.lastname: Sivakumar
authors.forename: Rajan
authors.initials: R
authors.lastname: Sriraman
authors.forename: Mohammed
authors.initials: M
authors.lastname: Raheem
authors.forename: Rajendra
authors.initials: R
authors.lastname: Lingala
authors.forename: Ramesh V
authors.initials: RV
authors.lastname: Matur
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D052162:Human papillomavirus 16 / Q000502:physiology*; D015151:Immunoblotting; D051379:Mice; D013050:Spectrometry, Fluorescence / Q000379:methods*; D019065:Virus Assembly
chemical_list: D036022:Capsid Proteins
keywords: ELISA; Fluorescence; SDS–PAGE; VLP disassembly and reassembly; Western blot