title: Differential chemical profiling to identify ozonation by-products of estrone-sulfate and first characterization of estrogenicity in generated drinking water.
abstract: For a few years, the concern of water treatment companies is not only focused on the removal of target micropollutants but has been extended to the investigation of potential biologically active by-products generated during the treatment processes. Therefore, some methods dedicated to the detection and structural characterization of such by-products have emerged. However, most of these studies are usually carried out under simplified conditions (e.g. high concentration levels of micropollutants, drastic treatment conditions, use of deionized or ultrapure water) and somewhat unrealistic conditions compared to that implemented in water treatment plants. In the present study, a real field water sample was fortified at the part-per-billion level (50 μg L(-1)) with estrone-3-sulfate (E1-3S) before being ozonated (at 1 mg L(-1)) for 10 min. In a first step, targeted measurements evidenced a degradation of the parent compound (>80%) in 10 min. Secondly, a non-targeted chemical profiling approach derived from metabolomic profiling studies allowed to reveal 11 ozonation by-products, among which 4 were found predominant. The estrogenic activity of these water samples spiked with E1-3S before and after treatment was assessed by the ER-CALUX assay and was found to decrease significantly after 10 min of ozonation. Therefore, this innovative methodological strategy demonstrated its suitability and relevancy for revealing unknown compounds generated from water treatment, and permitted to generate new results regarding specifically the impact of ozonation on estrone-3-sulfate. These results confirm that ozonation is effective at removing E1-3S in drinking water and indicate that the by-products generated have significantly lower estrogenic activity.
authors.affiliation: LUNAM Université, ONIRIS, Laboratoire d'Etude des Résidus et Contaminants dans les Aliments (LABERCA), Nantes, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Bourgin
authors.forename: Gaël
authors.initials: G
authors.lastname: Gervais
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Bichon
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Antignac
authors.forename: Fabrice
authors.initials: F
authors.lastname: Monteau
authors.forename: Gaëla
authors.initials: G
authors.lastname: Leroy
authors.forename: Lauriane
authors.initials: L
authors.lastname: Barritaud
authors.forename: Mathilde
authors.initials: M
authors.lastname: Chachignon
authors.forename: Valérie
authors.initials: V
authors.lastname: Ingrand
authors.forename: Pascal
authors.initials: P
authors.lastname: Roche
authors.forename: Bruno
authors.initials: B
authors.lastname: Le Bizec
mesh_terms: D005591:Chemical Fractionation; D060766:Drinking Water / Q000737:chemistry*; D052244:Endocrine Disruptors / Q000737:chemistry* / Q000494:pharmacology; D004970:Estrone / Q000031:analogs & derivatives* / Q000737:chemistry; D015394:Molecular Structure; D010126:Ozone / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods; D014874:Water Pollutants, Chemical / Q000737:chemistry* / Q000633:toxicity; D018508:Water Purification / Q000379:methods*
chemical_list: D060766:Drinking Water; D052244:Endocrine Disruptors; D014874:Water Pollutants, Chemical; D004970:Estrone; D010126:Ozone; C017296:estrone sulfate