title: Role of Moringa oleifera in regulation of diabetes-induced oxidative stress.
abstract: OBJECTIVE
  To evaluate the antioxidant activity of aqueous extract of Moringa oleifera (M. oleifera) young leaves by in vivo as well as in vitro assays.
  
  
  METHODS
  In vitro study included estimation of total phenolic, total flavonol, total flavonoid and total antioxidant power (FRAP assay). In addition, in vivo study was done with the identified most effective dose of 200 mg/kg of its lyophilized powder on normal and diabetic rats. Its effect on different oxidative free radical scavenging enzymes,viz, superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST), lipid peroxide (LPO) contents were measured.
  
  
  RESULTS
  Significant increase in activities of SOD, CAT, GST while, a decrease in LPO content was observed. Whereas, total phenolic, flavonoid and flavonol contents in the extract were found to be 120 mg/g of GAE, 40.5 mg/g of QE and 12.12 mg/g of QE, respectively. On the other hand, FRAP assay results of M. oleifera leaves was (85.00 ± 5.00) μM/g of extract powder.
  
  
  CONCLUSIONS
  The significant antioxidant activities of M. oleifera leaves from both in vivo as well as in vitro studies suggests that the regular intake of its leaves through diet can protect normal as well as diabetic patients against oxidative damage.
authors.affiliation: Alternative Therapeutics Unit, Drug Development Division, Medicinal Research Lab., Department of Chemistry, University of Allahabad, Allahabad-211 002, India.
authors.forename: Dolly
authors.initials: D
authors.lastname: Jaiswal
authors.forename: Prashant Kumar
authors.initials: PK
authors.lastname: Rai
authors.forename: Shikha
authors.initials: S
authors.lastname: Mehta
authors.forename: Sanjukta
authors.initials: S
authors.lastname: Chatterji
authors.forename: Surekha
authors.initials: S
authors.lastname: Shukla
authors.forename: Devendra Kumar
authors.initials: DK
authors.lastname: Rai
authors.forename: Gaurav
authors.initials: G
authors.lastname: Sharma
authors.forename: Bechan
authors.initials: B
authors.lastname: Sharma
authors.forename: Shahidul
authors.initials: S
authors.lastname: Khair
authors.forename: Geeta
authors.initials: G
authors.lastname: Watal
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000378:metabolism; D005419:Flavonoids / Q000032:analysis; D005982:Glutathione Transferase / Q000378:metabolism; D008054:Lipid Peroxides / Q000378:metabolism; D008297:Male; D029588:Moringa oleifera / Q000737:chemistry*; D009928:Organ Specificity; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000032:analysis; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D008054:Lipid Peroxides; D010636:Phenols; D010936:Plant Extracts; D002374:Catalase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase