title: Microalgae associated Brevundimonas sp. MSK 4 as the nano particle synthesizing unit to produce antimicrobial silver nanoparticles.
abstract: The biosynthesis of silver nanoparticles and its antimicrobial property was studied using bacteria isolated from Spirulina products. Isolated bacteria were identified as Bacillus sp. MSK 1 (JX495945), Staphylococcus sp. MSK 2 (JX495946), Bacillus sp. MSK 3 (JX495947) and Brevundimonas sp. MSK 4 (JX495948). Silver nanoparticles (AgNPs) were synthesized using bacterial culture filtrate with AgNO3. The initial syntheses of Ag nanoparticles were characterized by UV-vis spectrophotometer (by measuring the color change to intense brown). Fourier Transform Infrared Spectroscopy (FTIR) study showed evidence that proteins are possible reducing agents and Energy-dispersive X-ray (EDX) study showing the metal silver as major signal. The structure of AgNPs was determined by Scanning electron microscopy (SEM) and X-ray diffraction (XRD). Synthesized Ag nanoparticles with an average size of 40-65 nm have antimicrobial property against human pathogens like Proteus vulgaris, Salmonella typhi, Vibrio cholera, Streptococcus sp., Bacillus subtilis, Staphylococcus aureus, and Escherichia coli. Among the isolates Brevundimonas sp. MSK 4 alone showed good activity in both synthesis of AgNPs and antimicrobial activity. This work demonstrates the possible use of biological synthesized silver nanoparticles to combat the drug resistant problem.
authors.affiliation: Post Graduate and Research Department of Microbiology, Dr. N.G.P. Arts and Science College, Coimbatore - 48, Tamil Nadu, India.
authors.forename: Karthic
authors.initials: K
authors.lastname: Rajamanickam
authors.forename: S S
authors.initials: SS
authors.lastname: Sudha
authors.forename: Mebin
authors.initials: M
authors.lastname: Francis
authors.forename: T
authors.initials: T
authors.lastname: Sowmya
authors.forename: J
authors.initials: J
authors.lastname: Rengaramanujam
authors.forename: Periyasamy
authors.initials: P
authors.lastname: Sivalingam
authors.forename: Kandasamy
authors.initials: K
authors.lastname: Prabakar
mesh_terms: D000890:Anti-Infective Agents / Q000138:chemical synthesis* / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism*; D002244:Carbon / Q000494:pharmacology; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry*; D058086:Microalgae / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D012834:Silver / Q000494:pharmacology*; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D053545:Spirulina / Q000187:drug effects
chemical_list: D000890:Anti-Infective Agents; D012834:Silver; D002244:Carbon
keywords: AgNPs; Antimicrobial activity; Bacteria; EDX; Pathogens; SEM