title: A sensitive NADH and ethanol biosensor based on graphene-Au nanorods nanocomposites.
abstract: In this paper, a simple strategy for the synthesis of graphene-Au nanorods hybrid nanosheets (GN-AuNRs) through electrostatic interaction has been demonstrated. Due to the synergistic effect between AuNRs and GN, the hybrid nanosheets exhibited excellent performance toward dihydronicotinamide adenine dinucleotide (NADH) oxidation, with a low detection limit of 6 µM. The linear GN-AuNRs also served as a biocompatible and electroactive matrix for enzyme assembly to facilitate the electron transfer between the enzyme and the electrode. Using alcohol dehydrogenase (ADH) as a model system, a simple and effective sensing platform was developed for ethanol assay. The response displayed a good linear range from 5 to 377 µM with detection limit 1.5 μM. Furthermore, the interference effects of redox active substances, such as uric acid, ascorbic acid and glucose for the proposed biosensor were negligible.
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Hongmei
authors.initials: H
authors.lastname: Lu
authors.forename: Liu
authors.initials: L
authors.lastname: Deng
mesh_terms: D000327:Adsorption; D000426:Alcohol Dehydrogenase / Q000737:chemistry; D000434:Alcoholic Beverages / Q000032:analysis; D015374:Biosensing Techniques; D004800:Enzymes, Immobilized / Q000737:chemistry; D000431:Ethanol / Q000032:analysis* / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006108:Graphite / Q000737:chemistry; D009243:NAD / Q000032:analysis* / Q000737:chemistry; D053761:Nanocomposites / Q000737:chemistry; D043942:Nanotubes / Q000737:chemistry; D010084:Oxidation-Reduction
chemical_list: D004800:Enzymes, Immobilized; D009243:NAD; D000431:Ethanol; D006046:Gold; D006108:Graphite; D000426:Alcohol Dehydrogenase