title: Optimization of a method for the profiling and quantification of saponins in different green asparagus genotypes.
abstract: The main goal of this study was the optimization of a HPLC-MS method for the qualitative and quantitative analysis of asparagus saponins. The method includes extraction with aqueous ethanol, cleanup by solid phase extraction, separation by reverse phase chromatography, electrospray ionization, and detection in a single quadrupole mass analyzer. The method was used for the comparison of selected genotypes of Huétor-Tájar asparagus landrace and selected varieties of commercial diploid hybrids of green asparagus. The results showed that while protodioscin was almost the only saponin detected in the commercial hybrids, eight different saponins were detected in the Huétor-Tájar asparagus genotypes. The mass spectra indicated that HT saponins are derived from a furostan type steroidal genin having a single bond between carbons 5 and 6 of the B ring. The total concentration of saponins was found to be higher in triguero asparagus than in commercial hybrids.
authors.affiliation: Phytochemicals and Food Quality Group, Food Biotechnology Department, Instituto de la Grasa (CSIC), Seville, Spain.
authors.forename: Sara
authors.initials: S
authors.lastname: Vázquez-Castilla
authors.forename: Sara
authors.initials: S
authors.lastname: Jaramillo-Carmona
authors.forename: Jose María
authors.initials: JM
authors.lastname: Fuentes-Alventosa
authors.forename: Ana
authors.initials: A
authors.lastname: Jiménez-Araujo
authors.forename: Rocío
authors.initials: R
authors.lastname: Rodriguez-Arcos
authors.forename: Pedro
authors.initials: P
authors.lastname: Cermeño-Sacristán
authors.forename: Juan Antonio
authors.initials: JA
authors.lastname: Espejo-Calvo
authors.forename: Rafael
authors.initials: R
authors.lastname: Guillén-Bejarano
mesh_terms: D027761:Asparagus Plant / Q000737:chemistry* / Q000378:metabolism; D001947:Breeding; D005513:Food Inspection / Q000379:methods*; D018520:Plant Shoots / Q000737:chemistry* / Q000378:metabolism; D012503:Saponins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D013030:Spain; D013045:Species Specificity
chemical_list: D012503:Saponins