title: Rapid prototyping of a hybrid hierarchical polyurethane-cell/hydrogel construct for regenerative medicine.
abstract: The creation of branched vascular systems has attracted significant attention from both scientific and clinical areas. However, it is still a formidable challenge to build a three-dimensional (3-D) branched vascular system mimicking the native vascular systems with the traditional or existing fabrication methods. Here we demonstrate rapid manufacturing of a hybrid hierarchical polyurethane-cell/Hydrogel construct by a double-nozzle low-temperature deposition system. Based on this approach, a 3-D vascular template with both synthetic scaffold polymer and cell/hydrogel systems was constructed. The synthetic PU was used as an external scaffold material to provide mechanical support, while the gelatin/alginate/fibrinogen hydrogel was used as an internal scaffold material for adipose-derived stem cell (ADSC) accommodation. After the fabrication stages, the coherent 3-D composite construct was thawed at room temperature, crosslinked/polymerized with aqueous solutions, cultured in vitro under static or dynamic conditions, and embedded in vivo with stable architectures and excellent biocompatibilities. This technology will enable rapid manufacture of complex branched vascular templates for a wide array of scientific and clinical applications.
authors.affiliation: Key Laboratory for Advanced Materials Processing Technology, Ministry of Education & Center of Organ Manufacturing, Department of Mechanical Engineering, Tsinghua University, Beijing 100084, PR China.
authors.forename: Yuanwen
authors.initials: Y
authors.lastname: Huang
authors.forename: Kai
authors.initials: K
authors.lastname: He
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Wang
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D000464:Alginates / Q000737:chemistry; D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation; D002478:Cells, Cultured; D005337:Fibrin / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology; D051379:Mice; D008819:Mice, Nude; D011140:Polyurethanes / Q000737:chemistry*; D051381:Rats; D044968:Regenerative Medicine; D012867:Skin / Q000473:pathology; D013696:Temperature; D014183:Transplantation, Heterologous
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D011140:Polyurethanes; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D020723:Glucuronic Acid; D005780:Gelatin; D005337:Fibrin