title: Efficient secretion of (R)-3-hydroxybutyric acid from Halomonas sp. KM-1 cultured with saccharified Japanese cedar under microaerobic conditions.
abstract: In the presence of d-glucose, consumption of pentoses such as d-xylose is somewhat repressed by most bacteria. However, in Halomonas sp. KM-1, simultaneous utilization of a pure hexose and pentose for growth and PHB production has been observed. Moreover, this strain has been shown to preferentially utilize d-xylose from a mixture of hexose and pentose. In addition, the KM-1 strain produced (R)-3-hydroxybutyric acid ((R)-3-HB) by using saccharified Japanese cedar (Cryptomeria japonica) wood. The concentration of intracellular PHB after aerobic cultivation for 24h was 8.4 g/L, and after shifting to microaerobic conditions and further cultivation for 18 h, the concentration of (R)-3-HB in the medium reached 8.0 g/L. These results show that the KM-1 strain can efficiently utilize saccharified Japanese cedar and secreted (R)-3-HB under microaerobic conditions.
authors.affiliation: Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Midorigaoka, Ikeda, Osaka 563-8577, Japan. y-kawata@aist.go.jp
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Kawata
authors.forename: You-Xun
authors.initials: YX
authors.lastname: Jin
authors.forename: Masanobu
authors.initials: M
authors.lastname: Nojiri
mesh_terms: D020155:3-Hydroxybutyric Acid / Q000096:biosynthesis*; D000332:Aerobiosis; D050260:Carbohydrate Metabolism; D032485:Cryptomeria / Q000378:metabolism*; D020632:Halomonas / Q000378:metabolism*; D006601:Hexoses / Q000378:metabolism; D010429:Pentoses / Q000378:metabolism; D014934:Wood / Q000378:metabolism
chemical_list: D006601:Hexoses; D010429:Pentoses; D020155:3-Hydroxybutyric Acid