title: Id1 expression promotes peripheral CD4+ T cell proliferation and survival upon TCR activation without co-stimulation.
abstract: Although the role of E proteins in the thymocyte development is well documented, much less is known about their function in peripheral T cells. Here we demonstrated that CD4 promoter-driven transgenic expression of Id1, a naturally occurring dominant-negative inhibitor of E proteins, can substitute for the co-stimulatory signal delivered by CD28 to facilitate the proliferation and survival of naïve CD4+ cells upon anti-CD3 stimulation. We next discovered that IL-2 production and NF-κB activity after anti-CD3 stimulation were significantly elevated in Id1-expressing cells, which may be, at least in part, responsible for the augmentation of their proliferation and survival. Taken together, results from this study suggest an important role of E and Id proteins in peripheral T cell activation. The ability of Id proteins to by-pass co-stimulatory signals to enable T cell activation has significant implications in regulating T cell immunity.
authors.affiliation: Department of Immunology, Peking University Health Science Center, Beijing, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Liu
authors.forename: Rong
authors.initials: R
authors.lastname: Jin
authors.forename: Hong-Cheng
authors.initials: HC
authors.lastname: Wang
authors.forename: Hui
authors.initials: H
authors.lastname: Tang
authors.forename: Yuan-Feng
authors.initials: YF
authors.lastname: Liu
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Qian
authors.forename: Xiu-Yuan
authors.initials: XY
authors.lastname: Sun
authors.forename: Qing
authors.initials: Q
authors.lastname: Ge
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Sun
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D018106:CD28 Antigens / Q000378:metabolism; D017252:CD3 Complex / Q000378:metabolism; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology*; D049109:Cell Proliferation; D002469:Cell Separation; D002470:Cell Survival; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D051798:Inhibitor of Differentiation Protein 1 / Q000096:biosynthesis*; D007376:Interleukin-2 / Q000378:metabolism; D008213:Lymphocyte Activation; D051379:Mice; D008822:Mice, Transgenic; D016328:NF-kappa B / Q000378:metabolism; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism*
chemical_list: D018106:CD28 Antigens; D017252:CD3 Complex; C497455:Idb1 protein, mouse; D051798:Inhibitor of Differentiation Protein 1; D007376:Interleukin-2; D016328:NF-kappa B; D011948:Receptors, Antigen, T-Cell