title: Existence of antigen-specific immunoglobulin E is not sufficient for allergic nasal eosinophil infiltration in mice.
abstract: BACKGROUND
  Immunoglobulin E (IgE) is important for the development of allergic rhinitis (AR), though the contribution of IgE to the infiltration of eosinophils in the nasal mucosa has not been fully elucidated. In this study, antigen-induced sneezing and nasal eosinophil accumulation were comparatively investigated in anti-ovalbumin (OVA)-IgE transgenic (Tg) and wild-type (WT) mice.
  
  
  METHODS
  Tg and OVA-immunized WT mice were intranasally challenged with OVA. Antigen-specific serum IgE level, sneezing and infiltration of eosinophil into the nasal cavity were then examined.
  
  
  RESULTS
  The level of serum OVA-specific IgE in Tg mice was significantly higher than that in antigen-immunized WT mice. Compared to saline challenge, intranasal challenge with OVA significantly induced sneezing in both Tg and immunized WT mice. However, antigen-induced nasal eosinophil infiltration was observed in immunized WT mice but not in Tg mice.
  
  
  CONCLUSIONS
  IgE-mediated responses might not play a crucial role in antigen-induced eosinophil infiltration in AR.
authors.affiliation: Allergy and Immunology Project, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan.
authors.forename: Tomoe
authors.initials: T
authors.lastname: Nishimura
authors.forename: Mayumi
authors.initials: M
authors.lastname: Saeki
authors.forename: Osamu
authors.initials: O
authors.lastname: Kaminuma
authors.forename: Kunie
authors.initials: K
authors.lastname: Matsuoka
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Yonekawa
authors.forename: Akio
authors.initials: A
authors.lastname: Mori
authors.forename: Takachika
authors.initials: T
authors.lastname: Hiroi
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology*; D004804:Eosinophils / Q000276:immunology*; D005260:Female; D006967:Hypersensitivity / Q000276:immunology* / Q000473:pathology; D007073:Immunoglobulin E / Q000276:immunology*; D051379:Mice; D008822:Mice, Transgenic; D009297:Nasal Mucosa / Q000276:immunology* / Q000473:pathology; D010047:Ovalbumin / Q000276:immunology; D012221:Rhinitis, Allergic, Perennial / Q000276:immunology / Q000473:pathology
chemical_list: D000941:Antigens; D007073:Immunoglobulin E; D010047:Ovalbumin