title: Selectin blockade decreases postischemic recruitment of bone marrow stromal cells.
abstract: OBJECTIVE
  Investigate the localization mechanisms of bone marrow stromal cells following transient ischemia-reperfusion injury in a murine flap model.
  
  
  METHODS
  Controlled laboratory study.
  
  
  METHODS
  A cutaneous flap based on the inferior epigastric artery was elevated, and transient ischemia of 3.5 hours using a microvascular clamp was achieved. Fucoidan was injected intravenously 24 hours before the ischemic period. Following the period of ischemia, radiolabeled bone marrow stromal cells were injected intravenously, and radioactivity was determined postoperatively.
  
  
  RESULTS
  Attenuation of the uptake of bone marrow stromal cells into postischemic tissue was observed in those mice treated with fucoidan as indicated by gamma counts measured in the flaps when compared with controls (P < . 001).
  
  
  CONCLUSIONS
  Decreased uptake of radiolabeled bone marrow stromal cells into postischemic tissues pretreated with fucoidan indicates selectin-mediated bone-marrow stromal cell recruitment in a murine cutaneous flap model.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Louisiana State University-Shreveport Health Sciences Center, Shreveport, Louisiana, U.S.A.
authors.forename: Brian
authors.initials: B
authors.lastname: Lawton
authors.forename: Jennings R
authors.initials: JR
authors.lastname: Boyette
authors.forename: Melissa
authors.initials: M
authors.lastname: Hu
authors.forename: Timothy S
authors.initials: TS
authors.lastname: Lian
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000494:pharmacology; D016026:Bone Marrow Transplantation / Q000379:methods*; D002465:Cell Movement / Q000187:drug effects; D004195:Disease Models, Animal; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D019007:P-Selectin / Q000037:antagonists & inhibitors* / Q000378:metabolism; D011134:Polysaccharides / Q000494:pharmacology*; D015427:Reperfusion Injury / Q000378:metabolism / Q000628:therapy*; D013524:Surgical Flaps / Q000098:blood supply*
chemical_list: D000925:Anticoagulants; D019007:P-Selectin; D011134:Polysaccharides; C007789:fucoidan
keywords: Stem cells; fucoidan; ischemia-reperfusion; selectin