title: Structure of an A-form RNA duplex obtained by degradation of 6S RNA in a crystallization droplet.
abstract: In the course of a crystallographic study of a 132 nt variant of Aquifex aeolicus 6S RNA, a crystal structure of an A-form RNA duplex containing 12 base pairs was solved at a resolution of 2.6 Å. In fact, the RNA duplex is part of the 6S RNA and was obtained by accidental but precise degradation of the 6S RNA in a crystallization droplet. 6S RNA degradation was confirmed by microscopic observation of crystals and gel electrophoresis of crystallization droplets. The RNA oligomers obtained form regular A-form duplexes containing three GoU wobble-type base pairs, one of which engages in intermolecular contacts through a ribose-zipper motif at the crystal-packing interface.
authors.affiliation: Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, 7-1 Kioi-cho, Chiyoda-ku, Tokyo 102-8554, Japan. j.kondo@sophia.ac.jp
authors.forename: Jiro
authors.initials: J
authors.lastname: Kondo
authors.forename: Anne Catherine
authors.initials: AC
authors.lastname: Dock-Bregeon
authors.forename: Dagmar K
authors.initials: DK
authors.lastname: Willkomm
authors.forename: Roland K
authors.initials: RK
authors.lastname: Hartmann
authors.forename: Eric
authors.initials: E
authors.lastname: Westhof
mesh_terms: D003460:Crystallization; D009690:Nucleic Acid Conformation; D017433:Protein Structure, Secondary; D059748:Proteolysis; D012313:RNA / Q000737:chemistry / Q000235:genetics; D012329:RNA, Bacterial / Q000737:chemistry* / Q000235:genetics; D022661:RNA, Untranslated
chemical_list: C411065:6S RNA; D012329:RNA, Bacterial; D022661:RNA, Untranslated; D012313:RNA
keywords: 6S RNA; A-form RNA; RNA degradation