title: [Synchrotron radiation-based FTIR microspectroscopy study of 6-hydroxydopamine induced Parkinson's disease cell model].
abstract: SH-SY5Y cell line treated with 6-hydroxydopamine (6-OHDA) is a classical Parkinson's disease (PD). In the present study, synchrotron-based Fourier transform infrared (FTIR) microspectroscopy was used to analyze the biochemical composition of SH-SY5Y cell line treated with 6-OHDA. The detailed spectral analyses show the significant changes in cellular compositions such as lipids, proteins and nucleic acids in SH-SY5Y cells treated with 6-OHDA compared to control SH-SY5Y cells. As a result, the unsaturation levels of phospholipids decrease in SH-SY5Y cells treated with 6-OHDA compared to control cells, the analysis of protein secondary structure shows the significantly higher ratio of beta-sheet in PD cells compared to that of control cells, and the content of nuclear acid is highly decreased compared to that of control cells, suggesting that 6-OHDA induces the serious oxidative damage in SH-SY5Y cells. These findings suggest that SR-FTIR is an effective and precise technical tool to probe the biochemical changes of cells and then evaluate the pathological damage in cells.
authors.affiliation: National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, China. zhyred@shu.edu.cn
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Zhu
authors.forename: Xiao
authors.initials: X
authors.lastname: Pei
authors.forename: Ling-Yan
authors.initials: LY
authors.lastname: Wu
authors.forename: Ze-Ming
authors.initials: ZM
authors.lastname: Qi
authors.forename: Yu-Yin
authors.initials: YY
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Liu
authors.forename: Hou-Guang
authors.initials: HG
authors.lastname: Zhou
mesh_terms: D045744:Cell Line, Tumor; D006801:Humans; D009447:Neuroblastoma / Q000378:metabolism / Q000473:pathology*; D018384:Oxidative Stress; D016627:Oxidopamine / Q000633:toxicity*; D010300:Parkinson Disease / Q000473:pathology*; D017433:Protein Structure, Secondary; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
chemical_list: D016627:Oxidopamine