title: Endometrial dye instillation: a novel approach to histopathologic evaluation of morcellated hysterectomy specimens.
abstract: The purpose of this prospective pilot case study was to determine whether instillation of trypan blue dye into the uterine cavity before laparoscopic hysterectomy and morcellation aids in gross identification of endometrium. The most common commercially available trypan blue stain, VisionBlue was used in this study. Instillation was performed at the beginning of the procedure using an embryo transfer catheter. A sterile solution of trypan blue, 0.5 mL, was instilled transcervically into the uterine cavities in 12 patients before laparoscopic hysterectomy with uterine morcellation. The morcellated specimens were sent for routine gross pathologic and histologic examination. It was concluded that intrauterine instillation of trypan blue stained the endometrium, thus aiding the pathologist in identification of the endometrium in morcellated uterine specimens.
authors.affiliation: Division of Urogynecology and Minimally Invasive Gynecology, Penn State Milton S. Hershey Medical Center, Penn State College of Medicine, Department of Obstetrics and Gynecology, Hershey, Pennsylvania. Electronic address: mtam@hmc.psu.edu.
authors.forename: Teresa
authors.initials: T
authors.lastname: Tam
authors.forename: Gerald
authors.initials: G
authors.lastname: Harkins
authors.forename: Trevor
authors.initials: T
authors.lastname: Caldwell
authors.forename: Richard
authors.initials: R
authors.lastname: Zaino
authors.forename: Danielle
authors.initials: D
authors.lastname: Hazard
mesh_terms: D005260:Female; D006801:Humans; D007044:Hysterectomy / Q000379:methods*; D008875:Middle Aged; D011446:Prospective Studies; D014343:Trypan Blue; D014594:Uterine Neoplasms / Q000473:pathology* / Q000601:surgery; D014599:Uterus / Q000473:pathology* / Q000601:surgery
chemical_list: D014343:Trypan Blue
keywords: Hysterectomy; Laparoscopy; Leiomyoma; Morcellation; Pathology; Trypan blue; VisionBlue