title: An automated climbing apparatus to measure chemotherapy-induced neurotoxicity in Drosophila melanogaster.
abstract: We have developed a novel model system in Drosophila melanogaster to study chemotherapy-induced neurotoxicity in adult flies. Neurological deficits were measured using a manual geotactic climbing assay. The manual assay is commonly used; however, it is laborious, time-consuming, subject to human error and limited to observing one sample at a time. We have designed and built a new automated fly-counting apparatus that uses a "video capture-particle counting technology" to automatically measure 10 samples at a time, with 20 flies per sample. Climbing behavior was assessed manually, as in our previous studies, and with the automated apparatus within the same experiment yielding statistically similar results. Both climbing endpoints as well as the climbing rate can be measured in the apparatus, giving the assay more versatility than the manual assay. Automation of our climbing assay reduces variability, increases productivity and enables high throughput drug screens for neurotoxicity.
authors.affiliation: Department of Neurology; Mayo Clinic; Rochester, MN USA.
authors.forename: Jewel L
authors.initials: JL
authors.lastname: Podratz
authors.forename: Nathan P
authors.initials: NP
authors.lastname: Staff
authors.forename: Joshua B
authors.initials: JB
authors.lastname: Boesche
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Giorno
authors.forename: Matthew E
authors.initials: ME
authors.lastname: Hainy
authors.forename: Shaun A
authors.initials: SA
authors.lastname: Herring
authors.forename: Michael T
authors.initials: MT
authors.lastname: Klennert
authors.forename: Christian
authors.initials: C
authors.lastname: Milaster
authors.forename: Steve E
authors.initials: SE
authors.lastname: Nowakowski
authors.forename: Randall G
authors.initials: RG
authors.lastname: Krug
authors.forename: Ying
authors.initials: Y
authors.lastname: Peng
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Windebank
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D002945:Cisplatin / Q000633:toxicity*; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000502:physiology; D009068:Movement / Q000502:physiology; D020258:Neurotoxicity Syndromes / Q000175:diagnosis* / Q000209:etiology; D018675:Toxicity Tests / Q000295:instrumentation*
chemical_list: D000970:Antineoplastic Agents; D002945:Cisplatin
keywords: Drosophila; apparatus; automated; chemotherapy; cisplatin; climbing; geotactic; neurotoxicity