title: Histological evaluation of fresh frozen bone integration at different experimental times.
abstract: The aim of this work was to investigate, through histological evaluation, the in vivo behavior of fresh frozen bone (FFB) used as particulate bone substitute in intraoral regenerative procedures. A total of 10 patients (group 1) received particulate FFB graft for bone regeneration in postextractive sockets, and 10 (group 2) underwent maxillary sinus augmentation by using the same bone substitute as filling. Fresh frozen bone was supplied from the Tissue Bank of the Veneto Region, Treviso Section.Healing was uneventful for all the patients and was monitored by periodical radiographs. Patients were scheduled for implant insertion according to the radiographic aspect. However, the mean healing time for group 1 was 45 days, whereas for group 2 patients, it was 100 days. At the moment of implant insertion, bone specimens were collected at the site of implant placement, from both groups and processed for histological analysis.Histological analysis after hematoxylin-eosin staining obtained from group 1 patients showed the presence of newly formed bone tissue, still well distinguishable from the grafted bone substitute. In samples from group 2 patients, a better integration could be recognized associated with active bone remodeling phenomena.These results showed a good integration of the considered FFB graft within the host tissue both at 45 and 100 days after grafting, displaying this biomaterial as suitable for preimplant regenerative procedures.
authors.affiliation: Department of Medical, University "G. d'Annunzio," Chieti, Italy. tete@unich.it
authors.forename: Stefano
authors.initials: S
authors.lastname: Tetè
authors.forename: Vincenzo Luca
authors.initials: VL
authors.lastname: Zizzari
authors.forename: Ernesto
authors.initials: E
authors.lastname: D'Aloja
authors.forename: Raffaele
authors.initials: R
authors.lastname: Vinci
authors.forename: Susi
authors.initials: S
authors.lastname: Zara
authors.forename: Umberto
authors.initials: U
authors.lastname: Di Tore
authors.forename: Amelia
authors.initials: A
authors.lastname: Cataldi
authors.forename: Carmen
authors.initials: C
authors.lastname: Mortellaro
authors.forename: Enrico
authors.initials: E
authors.lastname: Gherlone
mesh_terms: D001672:Biocompatible Materials; D018786:Bone Substitutes; D016025:Bone Transplantation / Q000379:methods*; D019094:Dental Prosthesis, Implant-Supported / Q000379:methods*; D005260:Female; D005615:Freezing; D006801:Humans; D008297:Male; D008443:Maxillary Sinus / Q000601:surgery*; D008875:Middle Aged; D013516:Oral Surgical Procedures, Preprosthetic / Q000379:methods*; D016348:Osseointegration; D014945:Wound Healing
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes