title: Exploring the differences between mouse mAβ(1-42) and human hAβ(1-42) for Alzheimer's disease related properties and neuronal cytotoxicity.
abstract: The differences between mouse mAβ(1-42) and human hAβ(1-42), explored using CD and fluorescence spectroscopy, transmission electron microscopy, ROS fluorescent assay, and neuronal cell viability, revealed that mAβ(1-42) as a three-site mutant (R5G, Y10F and H13R) of hAβ(1-42) altered the metal (copper and zinc) binding sites, reduced the proneness to form β-sheet structures and aggregated fibrils, alleviated the generation of ROS, and decreased the cytotoxicity, in contrast to hAβ(1-42).
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Lv
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Luo
authors.forename: Dandan
authors.initials: D
authors.lastname: Wang
authors.forename: Cuiqing
authors.initials: C
authors.lastname: Zhu
authors.forename: Zhong-Xian
authors.initials: ZX
authors.lastname: Huang
authors.forename: Xiangshi
authors.initials: X
authors.lastname: Tan
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism / Q000473:pathology; D016229:Amyloid beta-Peptides / Q000235:genetics / Q000378:metabolism / Q000633:toxicity*; D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002942:Circular Dichroism; D003300:Copper / Q000378:metabolism; D006801:Humans; D051379:Mice; D009154:Mutation; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D010446:Peptide Fragments / Q000235:genetics / Q000378:metabolism / Q000633:toxicity*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013056:Spectrophotometry, Ultraviolet; D015032:Zinc / Q000378:metabolism
chemical_list: D016229:Amyloid beta-Peptides; D010446:Peptide Fragments; D017382:Reactive Oxygen Species; C075222:amyloid beta-protein (1-42); D003300:Copper; D015032:Zinc