title: Picosecond time-resolved FRET in the fluorescent protein from Discosoma Red (wt-DsRed).
abstract: Ultrafast, intra-oligomer fluorescence resonance energy transfer (FRET) between an immature green-emitting GFP-like chromophore to the mature red-emitting chromophore is found in the novel red fluorescent protein wt-DsRed (the picture shows the steady-state absorption (solid line) and emission (dotted) spectra). Since FRET is by its very nature a short range process, it represents a highly suitable method to probe oligomerization. This work describes a method preferentially applicable to the efficient screening of protein variants with mutagenetically altered surface docking sites.
authors.affiliation: Institut für Physikalische und Theoretische Chemie, Technische Universität München, Lichtenbergstrasse 4, 85748 Garching, Germany.
authors.forename: T A
authors.initials: TA
authors.lastname: Schüttrigkeit
authors.forename: U
authors.initials: U
authors.lastname: Zachariae
authors.forename: T
authors.initials: T
authors.lastname: von Feilitzsch
authors.forename: J
authors.initials: J
authors.lastname: Wiehler
authors.forename: J
authors.initials: J
authors.lastname: von Hummel
authors.forename: B
authors.initials: B
authors.lastname: Steipe
authors.forename: M E
authors.initials: ME
authors.lastname: Michel-Beyerle
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D037421:Anthozoa / Q000378:metabolism*; D031541:Fluorescence Resonance Energy Transfer; D007700:Kinetics; D008164:Luminescent Proteins / Q000737:chemistry* / Q000235:genetics; D013696:Temperature; D013997:Time Factors
chemical_list: D008164:Luminescent Proteins