title: Therapeutic efficacy of artemisinin combination therapies and prevalence of S769N mutation in PfATPase6 gene of Plasmodium falciparum in Kolkata, India.
abstract: OBJECTIVE
  To study the in vivo efficacy of these two ACTs in the treatment of Plasmodium falciparum (P. falciparum malaria) in Kolkata and to determine the prevalence of mutant S769N codon of the PfATPase6 gene among field isolates of P. falciparum collected from the study area.
  
  
  METHODS
  A total of 207P. falciparum positive cases were enrolled randomly in two study arms and followed up for 42 days as per WHO (2009) protocol. A portion of PfATPase6 gene spanning codon S769N was amplified and sequenced by direct sequencing method.
  
  
  RESULTS
  It was observed that the efficacy of both the ACT regimens were highly effective in the study area and no mutant S769N was detected from any isolate.
  
  
  CONCLUSIONS
  The used, combination AS+SP is effective and the other combination AM+LF might be an alternative, if needed.
authors.affiliation: Department of Microbiology, Calcutta School of Tropical Medicine, 108, C. R. Avenue, Kolkata 700 073, India.
authors.forename: Pabitra
authors.initials: P
authors.lastname: Saha
authors.forename: Arindam
authors.initials: A
authors.lastname: Naskar
authors.forename: Swagata
authors.initials: S
authors.lastname: Ganguly
authors.forename: Sonali
authors.initials: S
authors.lastname: Das
authors.forename: Subhasish K
authors.initials: SK
authors.lastname: Guha
authors.forename: Asit
authors.initials: A
authors.lastname: Biswas
authors.forename: Dilip K
authors.initials: DK
authors.lastname: Bera
authors.forename: Pratip K
authors.initials: PK
authors.lastname: Kundu
authors.forename: Madhusudan
authors.initials: M
authors.lastname: Das
authors.forename: Shrabanee
authors.initials: S
authors.lastname: Mullick
authors.forename: Krishnangshu
authors.initials: K
authors.lastname: Ray
authors.forename: Ardhendu K
authors.initials: AK
authors.lastname: Maji
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000962:Antimalarials / Q000008:administration & dosage*; D037621:Artemisinins / Q000008:administration & dosage*; D001483:Base Sequence; D000252:Calcium-Transporting ATPases / Q000235:genetics*; D002648:Child; D004351:Drug Resistance; D004359:Drug Therapy, Combination; D005260:Female; D017125:Genes, Protozoan; D006801:Humans; D007194:India; D016778:Malaria, Falciparum / Q000188:drug therapy / Q000469:parasitology*; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009154:Mutation; D010963:Plasmodium falciparum / Q000187:drug effects / Q000201:enzymology / Q000235:genetics*; D016896:Treatment Outcome
chemical_list: C513500:ATP6 protein, Plasmodium falciparum; D000962:Antimalarials; D037621:Artemisinins; C031327:artemisinin; D000252:Calcium-Transporting ATPases