title: Functional proteomics reveals the protective effects of saffron ethanolic extract on hepatic ischemia-reperfusion injury.
abstract: Hepatic ischemia-reperfusion (IR) injury is a common clinical problem and ROS may be a contributing factor on IR injury. The current study evaluates the potential protective effect of saffron ethanol extract (SEE) in a rat model upon hepatic IR injury. Caspases 3 and terminal deoxynucleotidyl transferase-mediated dUTP biotin nick end labeling (TUNEL) results showed increased cell death in the IR samples; reversely, minor apoptosis was detected in the SEE/IR group. Pretreatment with SEE significantly restored the content of antioxidant enzymes (SOD1 and catalase) and remarkably inhibited the intracellular ROS concentration in terms of reducing p47phox translocation. Proteome tools revealed that 20 proteins were significantly modulated in protein intensity between IR and SEE/IR groups. Particularly, SEE administration could attenuate the carbonylation level of several chaperone proteins. Network analysis suggested that saffron extract could alleviate IR-induced ER stress and protein ubiquitination, which finally lead to cell apoptosis. Taken together, SEE could reduce hepatic IR injury through modulating protein oxidation and our results might help to develop novel therapeutic strategies against ROS-caused diseases.
authors.affiliation: School of Traditional Chinese Medicine, Chang Gung University, Taoyuan, Taiwan. pan@mail.cgu.edu.tw
authors.forename: Tai-Long
authors.initials: TL
authors.lastname: Pan
authors.forename: Tung-Ho
authors.initials: TH
authors.lastname: Wu
authors.forename: Pei-Wen
authors.initials: PW
authors.lastname: Wang
authors.forename: Yann-Lii
authors.initials: YL
authors.lastname: Leu
authors.forename: Nardnisa
authors.initials: N
authors.lastname: Sintupisut
authors.forename: Chun-Hsun
authors.initials: CH
authors.lastname: Huang
authors.forename: Fang-Rong
authors.initials: FR
authors.lastname: Chang
authors.forename: Yang-Chang
authors.initials: YC
authors.lastname: Wu
mesh_terms: D000818:Animals; D002374:Catalase / Q000032:analysis / Q000378:metabolism; D027622:Crocus / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D000431:Ethanol; D006651:Histocytochemistry; D020287:In Situ Nick-End Labeling; D008099:Liver / Q000737:chemistry / Q000187:drug effects / Q000473:pathology; D008107:Liver Diseases / Q000378:metabolism*; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*; D050050:Protein Carbonylation; D011506:Proteins / Q000032:analysis / Q000378:metabolism; D020543:Proteome / Q000032:analysis / Q000378:metabolism*; D040901:Proteomics; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000032:analysis / Q000378:metabolism; D057149:Ubiquitinated Proteins
chemical_list: D010936:Plant Extracts; D020011:Protective Agents; D011506:Proteins; D020543:Proteome; D057149:Ubiquitinated Proteins; D000431:Ethanol; D002374:Catalase; D013482:Superoxide Dismutase
keywords: Animal proteomics; Antioxidant; Ischemia-reperfusion injury; Redox proteomics; Saffron; Valosin-containing protein