title: Direct intrathecal drug delivery in mice for detecting in vivo effects of cGMP on pain processing.
abstract: Intrathecal delivery of drugs is an important method in pain research in order to investigate pain-relevant effects in the spinal cord in vivo. Here, we describe a method of intrathecal drug delivery by direct lumbar puncture in mice. The procedure does not require surgery, is rapidly performed, and does not produce neurological deficits. If cGMP analogs are injected, a state of transient hindpaw hypersensitivity can be induced which is quantifiable by measurement of hindpaw withdrawal latency in response to mechanical stimulation.
authors.affiliation: Pharmazentrum Frankfurt/ZAFES, Institut für Klinische Pharmakologie, Klinikum der Johann Wolfgang Goethe-Universität, Frankfurt am Main, Germany.
authors.forename: Ruirui
authors.initials: R
authors.lastname: Lu
authors.forename: Achim
authors.initials: A
authors.lastname: Schmidtko
mesh_terms: D000818:Animals; D006152:Cyclic GMP / Q000008:administration & dosage* / Q000031:analogs & derivatives; D007278:Injections, Spinal; D008297:Male; D051379:Mice; D010146:Pain / Q000188:drug therapy*; D010147:Pain Measurement / Q000187:drug effects* / Q000379:methods*; D017288:Pain Threshold / Q000187:drug effects
chemical_list: D006152:Cyclic GMP