title: Aluminum inhibits root growth and induces hydrogen peroxide accumulation in Plantago algarbiensis and P. almogravensis seedlings.
abstract: We have evaluated the impact of aluminum (Al) on germination, relative root growth, Al accumulation in roots tips, H2O2 levels, plasma membrane integrity, pigment levels, protein content, and the activities of superoxide dismutase (SOD) and catalase (CAT) in seedlings of the endangered Portuguese species Plantago algarbiensis and Plantago almogravensis. We found that up to 400 μM Al had no impact on the germination percentage in either species but inhibited root growth in a concentration-dependent manner (more severely in P. algarbiensis). Al accumulation in the root tips of both species was concentration dependent up to 200 μM but declined thereafter despite the absence of membrane damage. We observed a concentration-dependent induction of SOD activity but no change in CAT activity resulting in the accumulation of H2O2 (a known growth inhibitor), although its impact in P. almogravensis may be partially ameliorated by the accumulation of carotenoid pigments. Our data suggest an association between Al uptake, H2O2 production, and the inhibition of root growth during early seedling development in P. algarbiensis and P. almogravensis, although the latter is more tolerant towards higher concentrations of the metal.
authors.affiliation: IBB/CGB, Faculty of Sciences and Technology, University of Algarve, Campus de Gambelas, Ed. 8, 8005-139, Faro, Portugal.
authors.forename: Neusa
authors.initials: N
authors.lastname: Martins
authors.forename: Sandra
authors.initials: S
authors.lastname: Gonçalves
authors.forename: Anabela
authors.initials: A
authors.lastname: Romano
mesh_terms: D000535:Aluminum / Q000633:toxicity*; D002374:Catalase / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D018525:Germination / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000378:metabolism*; D010788:Photosynthesis / Q000187:drug effects; D010860:Pigments, Biological / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D010943:Plantago / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D012995:Solubility; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D010860:Pigments, Biological; D010940:Plant Proteins; D006861:Hydrogen Peroxide; D000535:Aluminum; D002374:Catalase; D013482:Superoxide Dismutase