title: A gravity-induced flow injection system for surface plasmon resonance biosensor.
abstract: A number of portable surface plasmon resonance (SPR) devices have been developed for point-of-care (POC) testing. Meanwhile, micropumps have been fabricated to be integrated into these devices for flow injection analysis (FIA). However, the (micro) pumps, the tubes and their external control units were space-consuming. Here we developed a power-free flow injection analysis (FIA) method for SPR detection based on a gravity-induced flow injection (gFI) system. The gFI system was tubeless and did not need to be controlled. The fluid was driven into the detection areas by its own gravitational force. A transition channel was used to increase the liquid-level difference between the inlet reservoir and the outlet reservoir. After a liquid sample was placed in the inlet reservoir, the flow rate of the liquid sample was increased in the transition channel. Before it arrived at the sensing surface, the flow rate of the sample was steady (with an error of less than 10%). The fluctuation of the flow rate had an influence on the SPR response signal, which was successfully denoised using an internal reference. With the gFI system, the SPR imaging biosensor was able to perform real-time detection manually. The SPR responses of DNA hybridization and protein immobilization were successfully obtained.
authors.affiliation: Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhou
authors.forename: Ying
authors.initials: Y
authors.lastname: Mu
authors.forename: Mengchao
authors.initials: M
authors.lastname: Yang
authors.forename: Qi
authors.initials: Q
authors.lastname: Song
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhongyu
authors.initials: Z
authors.lastname: Wu
authors.forename: Liancheng
authors.initials: L
authors.lastname: Xiang
authors.forename: Wei
authors.initials: W
authors.lastname: Jin
authors.forename: Qinhan
authors.initials: Q
authors.lastname: Jin
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004247:DNA / Q000737:chemistry; D017022:Flow Injection Analysis / Q000295:instrumentation; D005978:Glutathione / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006112:Gravitation; D055767:Immobilized Proteins / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D019095:Point-of-Care Systems; D020349:Surface Plasmon Resonance
chemical_list: D055767:Immobilized Proteins; D006046:Gold; D004247:DNA; D005978:Glutathione