title: Purification, characterization and expression of two vitellogenins in the Indian freshwater murrel Channa punctatus.
abstract: The present study was undertaken to characterize different vitellogenins in Channa punctatus. Protein purification by gel chromatography followed by fast protein liquid chromatography (FPLC) revealed existence of two different Vg forms. Liquid chromatography tandem mass spectrophotometry (LC-MS/MS) suggested the existence of Vga and Vgb. Cloning of partial sequences of vga and vgb mRNA and phylogenetic analysis substantiated the existence of two vitellogenins. Real time PCR for vga and vgb genes from liver of estradiol-17β (E2) treated fish reveals difference in expression levels of transcripts of these two genes. vgb is expressed at lower dose of estradiol suggesting a higher sensitivity to estradiol. The present study thus proposes different regulatory control for the expression of these two genes and vgb as a superior biomarker than vga to assess exposure of C. punctatus to environmental estrogens.
authors.affiliation: Department of Zoology, Hindu College, University of Delhi, Delhi 110007, India.
authors.forename: V S
authors.initials: VS
authors.lastname: Rawat
authors.forename: S
authors.initials: S
authors.lastname: Pipil
authors.forename: L
authors.initials: L
authors.lastname: Sharma
authors.forename: N
authors.initials: N
authors.lastname: Sehgal
mesh_terms: D000818:Animals; D005618:Fresh Water; D010473:Perciformes / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D053719:Tandem Mass Spectrometry; D014819:Vitellogenins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*
chemical_list: D014819:Vitellogenins