title: Mechanistic study of a diazo dye degradation by Soybean Peroxidase.
abstract: BACKGROUND
  Enzyme based remediation of wastewater is emerging as a novel, efficient and environmentally-friendlier approach. However, studies showing detailed mechanisms of enzyme mediated degradation of organic pollutants are not widely published.
  
  
  RESULTS
  The present report describes a detailed study on the use of Soybean Peroxidase to efficiently degrade Trypan Blue, a diazo dye. In addition to examining various parameters that can affect the dye degradation ability of the enzyme, such as enzyme and H2O2 concentration, reaction pH and temperature, we carried out a detailed mechanistic study of Trypan Blue degradation. HPLC-DAD and LC-MS/MS studies were carried out to confirm dye degradation and analyze the intermediate metabolites and develop a detailed mechanistic dye degradation pathway.
  
  
  CONCLUSIONS
  We report that Soybean peroxidase causes Trypan Blue degradation via symmetrical azo bond cleavage and subsequent radical-initiated ring opening of the metabolites. Interestingly, our results also show that no high molecular weight polymers were produced during the peroxidase-H2O2 mediated degradation of the phenolic Trypan Blue.
authors.affiliation: Department of Chemistry, UAE University, P, O, Box 15551, Al-Ain, United Arab Emirates. salman.ashraf@uaeu.ac.ae.
authors.forename: Umme
authors.initials: U
authors.lastname: Kalsoom
authors.forename: Syed Salman
authors.initials: SS
authors.lastname: Ashraf
authors.forename: Mohammed A
authors.initials: MA
authors.lastname: Meetani
authors.forename: Muhammad A
authors.initials: MA
authors.lastname: Rauf
authors.forename: Haq Nawaz
authors.initials: HN
authors.lastname: Bhatti