title: Home-made rapid urease test compared with commercial kit for diagnosis of Helicobacter pylori infection.
abstract: OBJECTIVE
  To evaluate the sensitivity and specificity of home-made rapid urease test compared with commercial kit for diagnosis of Helicobacter pylori infection.
  
  
  METHODS
  A cross-sectional study of patients who underwent esophagogastro-duodenoscopy from June 2009 to May 2010 was carried out. Gastric biopsy specimens were taken from antrum and body of the stomach for home-made rapid urease testing, commercial kit (hpfast) urease testing, and histological study.
  
  
  RESULTS
  One hundred and ninety nine patients were included in the present study. The sensitivity of home-made rapid urease test and hpfast were 40% and 48.5% at 1 hour and 91.4% and 91.4% at 24 hours. The specificity, positive and negative predictive value and accuracy of home-made rapid urease test vs. hpfast were 100% vs. 100%, 100% vs. 100%, 95.5% vs. 95.5% and 96.9% vs. 96.9% respectively. Furthermore 45.3% of home-made rapid urease test and 54. 7% of hpfast showed positive results within 1 hour
  
  
  CONCLUSIONS
  Home-made rapid urease test has good sensitivity and specificity comparable to commercial kit for the diagnosis of H. pylori infection.
authors.affiliation: Division of Gastroenterology, Department of Internal Medicine, Faculty of Medicine, Srinakharinwirot University, Nakhon Nayok, Thailand. cwongjitrat@yahoo.com
authors.forename: Chatchawan
authors.initials: C
authors.lastname: Wongjitrat
authors.forename: Nattakarn
authors.initials: N
authors.lastname: Wongjitrat
mesh_terms: D005260:Female; D016481:Helicobacter Infections / Q000175:diagnosis*; D016480:Helicobacter pylori; D006801:Humans; D008297:Male; D008875:Middle Aged; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D014510:Urease
chemical_list: D011933:Reagent Kits, Diagnostic; D014510:Urease