title: Challenges of "sticky" co-immunoprecipitation: polyalanine tract protein-protein interactions.
abstract: Co-immunoprecipitation (Co-IP) (followed by immunoblotting) is a technique widely used to characterize specific protein-protein interactions. Investigating interactions of proteins containing "sticky" polyalanine (PolyA) tracts encounters difficulties using conventional Co-IP procedures. Here, we present strategies to specifically capture proteins containing these difficult PolyA tracts, enabling subsequent robust detection of interacting proteins by Co-IP.
authors.affiliation: Department of Paediatrics, University of Adelaide, Adelaide, SA, Australia.
authors.forename: T R
authors.initials: TR
authors.lastname: Mattiske
authors.forename: May H
authors.initials: MH
authors.lastname: Tan
authors.forename: Jozef
authors.initials: J
authors.lastname: Gécz
authors.forename: Cheryl
authors.initials: C
authors.lastname: Shoubridge
mesh_terms: D000818:Animals; D002460:Cell Line; D018398:Homeodomain Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods*; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism
chemical_list: C469264:ARX protein, human; D018398:Homeodomain Proteins; D010455:Peptides; D014157:Transcription Factors; C019529:polyalanine