title: In vitro inhibition of vesicular stomatitis virus replication by purified porcine Mx1 protein fused to HIV-1 Tat protein transduction domain (PTD).
abstract: Vesicular stomatitis virus (VSV) is the causative agent of Vesicular stomatitis (VS), a highly contagious fatal disease of human and pigs. Few effective antiviral drugs are currently available against VSV infection. Mx proteins are interferon (IFN)-induced dynamin-like GTPases present in all vertebrates with a range of antiviral activities. Previous studies have shown that the transfected cell lines expressing either porcine Mx1 or human MxA acquired a high degree of resistance to VSV. To explore the feasibility of taking porcine Mx1 protein expressed in Escherichia coli as an antiviral agent, we applied the pCold system to express this fusion protein (PTD-poMx1), which consisted of an N-terminal HIV-1 Tat protein transduction domain (PTD) and the full-length porcine Mx1, and investigated its effects on the replication of VSV in Vero cells. The results demonstrated that the purified PTD-poMx1 fusion proteins could transduct into cells after incubated for 5h and had no cytotoxic. Furthermore, plaque reduction assay, determination of TCID50, real-time PCR and Western blot analyses were carried out to confirm the antiviral activity of purified fusion proteins in VSV-infected Vero cells. Altogether, these data suggested that PTD-poMx1 fusion proteins might be applicable to inhibit VSV replication as a novel antiviral therapeutic agent.
authors.affiliation: Key Laboratory of Animal Diseases Diagnosis and Immunology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Xiao-min
authors.initials: XM
authors.lastname: Zhang
authors.forename: Dan-Ni
authors.initials: DN
authors.lastname: He
authors.forename: Bin
authors.initials: B
authors.lastname: Zhou
authors.forename: Ran
authors.initials: R
authors.lastname: Pang
authors.forename: Ke
authors.initials: K
authors.lastname: Liu
authors.forename: Jin
authors.initials: J
authors.lastname: Zhao
authors.forename: Pu-yan
authors.initials: PY
authors.lastname: Chen
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D002460:Cell Line; D002522:Chlorocebus aethiops; D064547:Myxovirus Resistance Proteins / Q000235:genetics / Q000494:pharmacology*; D011993:Recombinant Fusion Proteins / Q000494:pharmacology; D014709:Vero Cells; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects* / Q000502:physiology; D014779:Virus Replication / Q000187:drug effects*; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000235:genetics / Q000494:pharmacology*
chemical_list: D000998:Antiviral Agents; D064547:Myxovirus Resistance Proteins; D011993:Recombinant Fusion Proteins; D054322:tat Gene Products, Human Immunodeficiency Virus
keywords: Antiviral activity; Porcine Mx1 (poMx1); Protein transduction domain (PTD); Vesicular stomatitis virus (VSV)