title: Hydroxyapatite synthesis using EDTA.
abstract: Bone comprises structure of the body and consisted of inorganic substances. It exists in an organic structure in the body. Even though it is firm and has self-healing mechanism, it can be damaged by trauma, cancer, or bone diseases. Allograft can be an alternative solution for autologous bone graft. Hydroxyapatite (Ca10(PO4)6(OH)2), an excellent candidate for allograft, can be applied to bone defect area. There are several methods to produce hydroxyapatite; however, economical cost and being time consuming make the production difficult. In this study, we synthesized hydroxyapatite with EDTA. Freeze-dried bone allograft (Hans Biomed) was used as the control group. Synthesized hydroxyapatite was a rod-shaped, white powdery substance with 2- to 5-μm length and 0.5- to 1-μm width. X-ray diffraction showed the highest sharp peak at 32°C and high peaks at 25.8°C, 39.8°C, 46.8°C, 49.5°C, and 64.0°C, indicating a similar substance to the freeze-dried bone allograft. After 3 days, the cell growth of synthesized hydroxyapatite showed 1.5-fold more than did the bone allograft. Cellular and media alkaline phosphate activity increased similar to the bone allograft. In this study, we came up with a new method to produce the hydroxyapatite. It is a convenient method that can be held in room temperature and low pressure. Also, the product can be manufactured in large quantity. It can be also transformed into scaffold structure, which will perform a stronger configuration. The manufacturing method will help the bony defect patients and make future medical products.
authors.affiliation: Department of Plastic & Reconstructive Surgery, College of Medicine, Chungnam National University, Daejeon, Korea.
authors.forename: Nak Heon
authors.initials: NH
authors.lastname: Kang
authors.forename: Soon Je
authors.initials: SJ
authors.lastname: Kim
authors.forename: Seung Han
authors.initials: SH
authors.lastname: Song
authors.forename: Sang mun
authors.initials: Sm
authors.lastname: Choi
authors.forename: Sik Young
authors.initials: SY
authors.lastname: Choi
authors.forename: Youn Jung
authors.initials: YJ
authors.lastname: Kim
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000032:analysis; D064591:Allografts / Q000737:chemistry; D000818:Animals; D001842:Bone and Bones / Q000737:chemistry; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D003461:Crystallography; D017886:Durapatite / Q000138:chemical synthesis* / Q000737:chemistry; D004492:Edetic Acid / Q000737:chemistry*; D005612:Freeze Drying; D051379:Mice; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000737:chemistry / Q000502:physiology; D016062:Porosity; D011312:Pressure; D013050:Spectrometry, Fluorescence; D013696:Temperature; D054457:Tissue Scaffolds / Q000737:chemistry; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: D017886:Durapatite; D004492:Edetic Acid; D000469:Alkaline Phosphatase