title: Laser-activated adhesive films for sutureless median nerve anastomosis.
abstract: A novel chitosan adhesive film that incorporates the dye 'Rose Bengal' (RB) was used in conjunction with a green laser to repair transected rat median nerves in vivo. Histology and electrophysiological recording assessed the impact of the laser-adhesive technique on nerves. One week post-operatively, the sham-control group (laser-adhesive technique applied on un-transected nerves) conserved the average number and size of myelinated fibres in comparison to its contralateral side and electrophysiological recordings demonstrated no significant difference with un-operated nerves. Twelve weeks after the laser-adhesive anastomoses, nerves were in continuity with regenerated axons that crossed the anastomotic site.
authors.affiliation: School of Medicine, University of Western Sydney, Locked Bag 1797 Penrith, NSW, 2751, Australia.
authors.forename: Mathew
authors.initials: M
authors.lastname: Barton
authors.forename: John W
authors.initials: JW
authors.lastname: Morley
authors.forename: Marcus A
authors.initials: MA
authors.lastname: Stoodley
authors.forename: Kheng-Seong
authors.initials: KS
authors.lastname: Ng
authors.forename: Sabine C
authors.initials: SC
authors.lastname: Piller
authors.forename: Hong
authors.initials: H
authors.lastname: Duong
authors.forename: Damia
authors.initials: D
authors.lastname: Mawad
authors.forename: David A
authors.initials: DA
authors.lastname: Mahns
authors.forename: Antonio
authors.initials: A
authors.lastname: Lauto
mesh_terms: D000269:Adhesives / Q000494:pharmacology*; D000714:Anastomosis, Surgical / Q000379:methods*; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology; D048271:Chitosan / Q000494:pharmacology; D055724:Electrophysiological Phenomena / Q000187:drug effects; D005260:Female; D066298:In Vitro Techniques; D007834:Lasers; D008475:Median Nerve / Q000166:cytology / Q000187:drug effects* / Q000502:physiology / Q000601:surgery*; D051381:Rats; D013718:Tensile Strength
chemical_list: D000269:Adhesives; D001672:Biocompatible Materials; D048271:Chitosan
keywords: Photochemical tissue bonding; Rose Bengal; chitosan; peripheral nerve repair