title: Down-regulation of long non-coding RNA TUG1 inhibits osteosarcoma cell proliferation and promotes apoptosis.
abstract: OBJECTIVE
  To investigate the expression level of TUG1 and one of its transcript variants (n377360) in osteosarcoma cells and assess the role of TUG1 in proliferation and apoptosis in the U2OS cell line.
  
  
  METHODS
  TUG1 and n377360 expression levels in patients with osteosarcomas and the U2OS human osteosarcoma cell line were evaluated using real-time quantitative PCR. U2OS cells were transected with TUG1 and n377360 siRNA or non-targeting siRNA. MTS was performed to assess the cell proliferation and flow cytometry was applied to analyze apoptosis.
  
  
  RESULTS
  We found significantly higher TUG1 and n377360 expression levels in osteosarcoma tissues compared with matched non-tumorous tissues. In line with this, suppression of TUG1 and n377360 expression by siRNA significantly impaired the cell proliferation potential of osteosarcoma cells. Furthermore, inhibition of TUG1 expression significantly promoted osteosarcoma cell apoptosis.
  
  
  CONCLUSIONS
  The overexpression of TUG1 and n377360 in osteosarcoma specimens and the functional role of TUG1 and n377360 regarding cell proliferation and apoptosis in an osteosarcoma cell line provided evidence that the use of TUG1 or n377360 may be a viable but an as yet unexplored therapeutic strategy in tumors that over express these factors.
authors.affiliation: Department of Orthopedics, Chinese PLA General Hospital, Beijing, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.forename: Pei-Liang
authors.initials: PL
authors.lastname: Geng
authors.forename: Pei
authors.initials: P
authors.lastname: Yin
authors.forename: Xiao-Lin
authors.initials: XL
authors.lastname: Wang
authors.forename: Jin-Peng
authors.initials: JP
authors.lastname: Jia
authors.forename: Jie
authors.initials: J
authors.lastname: Yao
mesh_terms: D000293:Adolescent; D000328:Adult; D017209:Apoptosis; D001859:Bone Neoplasms / Q000235:genetics / Q000473:pathology*; D001842:Bone and Bones / Q000378:metabolism* / Q000473:pathology; D049109:Cell Proliferation; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D008297:Male; D012516:Osteosarcoma / Q000235:genetics / Q000473:pathology*; D011379:Prognosis; D062085:RNA, Long Noncoding / Q000037:antagonists & inhibitors* / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics*; D012189:Retrospective Studies; D014407:Tumor Cells, Cultured; D055815:Young Adult
chemical_list: D062085:RNA, Long Noncoding; D034741:RNA, Small Interfering