title: Application of yeast Candida utilis to ferment Eisenia bicyclis for enhanced antibacterial effect.
abstract: In this study, fermentation broth of Eisenia bicyclis with Candia utilis YM-1 exhibited enhanced antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA) and food-borne pathogenic bacteria. To perform a more detailed investigation on the antibacterial activity, the fermented broth of E. bicyclis was extracted with methanol and further fractionated with organic solvents. After 1-day fermentation, the ethyl acetate (EtOAc)-soluble extract exhibited the highest anti-MRSA activity with minimum inhibitory concentration values ranging from 128 to 512 μg/mL, suggesting that the fermentation of E. bicyclis with C. utilis YM-1 may enhance antibacterial activity against MRSA. This effect was correlated to the result obtained by an increase in total phenolic contents in EtOAc-soluble extract. In addition, high-performance liquid chromatography analysis revealed that eckol, dieckol, dioxinodehydroeckol, and phlorofucofuroeckol-A contents in the EtOAc-soluble extract increased significantly. Thus, these results show that anti-MRSA activity of E. bicyclis fermented with C. utilis most likely originated from phlorotannins and allow the possible application of a variety of seaweed functional foods.
authors.affiliation: Department of Food Science and Technology, Pukyong National University, Busan, 608-737, Republic of Korea.
authors.forename: Sung-Hwan
authors.initials: SH
authors.lastname: Eom
authors.forename: Dae-Sung
authors.initials: DS
authors.lastname: Lee
authors.forename: Young Mi
authors.initials: YM
authors.lastname: Kang
authors.forename: Kwang-Tae
authors.initials: KT
authors.lastname: Son
authors.forename: You-Jin
authors.initials: YJ
authors.lastname: Jeon
authors.forename: Young-Mog
authors.initials: YM
authors.lastname: Kim
mesh_terms: D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology; D002175:Candida / Q000378:metabolism*; D005285:Fermentation; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D000459:Phaeophyceae / Q000737:chemistry / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology; D012624:Seaweed / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts