title: Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease.
abstract: We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in Drosophila.
authors.affiliation: Genetics Training Program, University of Wisconsin, Madison, Madison, WI 53706, USA.
authors.forename: Scott J
authors.initials: SJ
authors.lastname: Gratz
authors.forename: Alexander M
authors.initials: AM
authors.lastname: Cummings
authors.forename: Jennifer N
authors.initials: JN
authors.lastname: Nguyen
authors.forename: Danielle C
authors.initials: DC
authors.lastname: Hamm
authors.forename: Laura K
authors.initials: LK
authors.lastname: Donohue
authors.forename: Melissa M
authors.initials: MM
authors.lastname: Harrison
authors.forename: Jill
authors.initials: J
authors.lastname: Wildonger
authors.forename: Kate M
authors.initials: KM
authors.lastname: O'Connor-Giles
mesh_terms: D000818:Animals; D064113:CRISPR-Cas Systems; D064112:Clustered Regularly Interspaced Short Palindromic Repeats; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics; D004706:Endodeoxyribonucleases / Q000235:genetics / Q000378:metabolism*; D018390:Gene Targeting; D005818:Genetic Engineering / Q000379:methods*; D049750:Genome, Insect; D018095:Germ-Line Mutation; D059765:Homologous Recombination
chemical_list: D029721:Drosophila Proteins; C050680:y protein, Drosophila; D004706:Endodeoxyribonucleases
keywords: CRISPR RNA; Cas9; Drosophila; genome engineering; homologous recombination