title: Structure-activity relationships of cecropin-like peptides and their interactions with phospholipid membrane.
abstract: Cecropin A and papiliocin are novel 37-residue cecropin-like antimicrobial peptides isolated from insect. We have confirmed that papiliocin possess high bacterial cell selectivity and has an α-helical structure from Lys(3) to Lys(21) and from Ala(25) to Val(35), linked by a hinge region. In this study, we demonstrated that both peptides showed high antimicrobial activities against multi-drug resistant Gram negative bacteria as well as fungi. Interactions between these cecropin-like peptides and phospholipid membrane were studied using CD, dye leakage experiments, and NMR experiments, showing that both peptides have strong permeabilizing activities against bacterial cell membranes and fungal membranes as well as Trp(2) and Phe(5) at the N-terminal helix play an important role in attracting cecropin-like peptides to the negatively charged bacterial cell membrane. Cecropin-like peptides can be potent peptide antibiotics against multi-drug resistant Gram negative bacteria and fungi.
authors.affiliation: Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Institute of SMART Biotechnology, Konkuk University, Seoul 143-701.
authors.forename: Eunjung
authors.initials: E
authors.lastname: Lee
authors.forename: Ki-Woong
authors.initials: KW
authors.lastname: Jeong
authors.forename: Juho
authors.initials: J
authors.lastname: Lee
authors.forename: Areum
authors.initials: A
authors.lastname: Shin
authors.forename: Jin-Kyoung
authors.initials: JK
authors.lastname: Kim
authors.forename: Juneyoung
authors.initials: J
authors.lastname: Lee
authors.forename: Dong Gun
authors.initials: DG
authors.lastname: Lee
authors.forename: Yangmee
authors.initials: Y
authors.lastname: Kim
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D002460:Cell Line; D002942:Circular Dichroism; D005453:Fluorescence; D005658:Fungi / Q000187:drug effects; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008563:Membrane Lipids / Q000737:chemistry* / Q000378:metabolism; D008954:Models, Biological; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism; D010743:Phospholipids / Q000737:chemistry* / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D008563:Membrane Lipids; D010455:Peptides; D010743:Phospholipids; C031161:cecropin A