title: [Effects of glimepiride on proliferation, differentiation and mineralization of rat mandibular osteoblasts in hyperglycemia].
abstract: OBJECTIVE
  To evaluate the effects of hyperglycemia and glimepiride on proliferation, differentiation and mineralization of rat mandibular osteoblasts to verify the hypothesis of dental implant administration.
  
  
  METHODS
  Primary osteoblasts were isolated and cultured. Then the cells were placed in an osteogenic medium, containing 2 different concentrations of glucose (5.5 mmol/L and 16.5 mmol/L), with or without glimepiride (10 μmol/L). Cell proliferation was evaluated through MTT assay. Alkaline phosphatase (ALP) activity was determined by biochemistric method. Col I protein levels were determined by Western blot. OCN mRNA levels were tested by RT-PCR. SPSS 13.0 software package was used for statistical analysis.
  
  
  RESULTS
  Hyperglycemic conditions interfered with the proliferation, ALP activity and OCN mRNA expression of rat osteoblasts, but improved the expression of Col I on day 14. Glimepiride stimulated rat osteoblast proliferation, ALP activity and OCN mRNA expression. The addition of glimepiride to normoglycemic (5.5 mmol/L) cultures registered a significant increase of Col I expression at 7 d and 14 d. Glimepiride significantly increased Col I expression in cells cultured with 16.5 mmol/L glucose for 7 days, but failed to increase at 14 d.
  
  
  CONCLUSIONS
  Hyperglycemic conditions interfered with the proliferation, differentiation and mineralization of osteoblasts in rats; however, glimepiride improved the proliferation, differentiation and mineralization of osteoblasts in rats.
authors.affiliation: Department of Oral Implantology, Beijing Stomatological Hospital of Capital Medical University. Beijing, China. mapanxw@163.com
authors.forename: Pan
authors.initials: P
authors.lastname: Ma
authors.forename: Bao-sheng
authors.initials: BS
authors.lastname: Tan
authors.forename: Hong-chen
authors.initials: HC
authors.lastname: Liu
authors.forename: Bin
authors.initials: B
authors.lastname: Gu
authors.forename: Jun-li
authors.initials: JL
authors.lastname: Ma
authors.forename: Ling-ling
authors.initials: LL
authors.lastname: E
authors.forename: Xia
authors.initials: X
authors.lastname: Wu
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D006943:Hyperglycemia; D008334:Mandible; D010006:Osteoblasts; D012333:RNA, Messenger; D051381:Rats; D013453:Sulfonylurea Compounds
chemical_list: D012333:RNA, Messenger; D013453:Sulfonylurea Compounds; C057619:glimepiride