title: Deletion of virulence associated genes from attenuated African swine fever virus isolate OUR T88/3 decreases its ability to protect against challenge with virulent virus.
abstract: African swine fever virus (ASFV) causes an acute haemorrhagic disease of domestic pigs against which there is no effective vaccine. The attenuated ASFV strain OUR T88/3 has been shown previously to protect vaccinated pigs against challenge with some virulent strains including OUR T88/1. Two genes, DP71L and DP96R were deleted from the OUR T88/3 genome to create recombinant virus OUR T88/3ΔDP2. Deletion of these genes from virulent viruses has previously been shown to reduce ASFV virulence in domestic pigs. Groups of 6 pigs were immunised with deletion virus OUR T88/3ΔDP2 or parental virus OUR T88/3 and challenged with virulent OUR T88/1 virus. Four pigs (66%) were protected by inoculation with the deletion virus OUR T88/3ΔDP2 compared to 100% protection with the parental virus OUR T88/3. Thus the deletion of the two genes DP71L and DP96R from OUR T88/3 strain reduced its ability to protect pigs against challenge with virulent virus.
authors.affiliation: The Pirbright Institute, Ash Road, Pirbright, Woking, Surrey, GU24 0NF, UK. charles.abrams@pirbright.ac.uk
authors.forename: Charles C
authors.initials: CC
authors.lastname: Abrams
authors.forename: Lynnette
authors.initials: L
authors.lastname: Goatley
authors.forename: Emma
authors.initials: E
authors.lastname: Fishbourne
authors.forename: David
authors.initials: D
authors.lastname: Chapman
authors.forename: Lyndsay
authors.initials: L
authors.lastname: Cooke
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Oura
authors.forename: Christopher L
authors.initials: CL
authors.lastname: Netherton
authors.forename: Haru-Hisa
authors.initials: HH
authors.lastname: Takamatsu
authors.forename: Linda K
authors.initials: LK
authors.lastname: Dixon
mesh_terms: D000357:African Swine Fever / Q000276:immunology / Q000473:pathology / Q000517:prevention & control* / Q000821:virology; D000358:African Swine Fever Virus / Q000235:genetics* / Q000276:immunology*; D000818:Animals; D017384:Sequence Deletion; D012720:Severity of Illness Index; D013154:Spleen / Q000821:virology; D013552:Swine; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D014766:Viremia / Q000276:immunology / Q000517:prevention & control; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics* / Q000276:immunology*
chemical_list: D014613:Vaccines, Attenuated; D014765:Viral Vaccines; D037521:Virulence Factors