title: Rapid identification of pathogens directly from blood culture bottles by Bruker matrix-assisted laser desorption laser ionization-time of flight mass spectrometry versus routine methods.
abstract: The use of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for identification of microorganisms directly from blood culture is an exciting dimension to the microbiologists. We evaluated the performance of Bruker SepsiTyper kit™ (STK) for direct identification of bacteria from positive blood culture. This was done in parallel with conventional methods. Nonrepetitive positive blood cultures from 160 consecutive patients were prospectively evaluated by both methods. Of 160 positive blood cultures, the STK identified 114 (75.6%) isolates and routine conventional method 150 (93%). Thirty-six isolates were misidentified or not identified by the kit. Of these, 5 had score of >2.000 and 31 had an unreliable low score of <1.7. Four of 8 yeasts were identified correctly. The average turnaround time using the STK was 35 min, including extraction steps and 30:12 to 36:12 h with routine method. The STK holds promise for timely management of bacteremic patients.
authors.affiliation: Department of Microbiology Faculty of Medicine, Kuwait University, Safat 13110, Kuwait. wjamal@hsc.edu.kw
authors.forename: Wafaa
authors.initials: W
authors.lastname: Jamal
authors.forename: Rola
authors.initials: R
authors.lastname: Saleem
authors.forename: Vincent O
authors.initials: VO
authors.lastname: Rotimi
mesh_terms: D016470:Bacteremia / Q000175:diagnosis / Q000382:microbiology*; D015373:Bacterial Typing Techniques; D002175:Candida / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D003470:Culture Media; D016469:Fungemia / Q000175:diagnosis / Q000382:microbiology*; D006090:Gram-Negative Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006094:Gram-Positive Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D016533:Mycological Typing Techniques; D012336:RNA, Ribosomal, 16S / Q000145:classification / Q000235:genetics*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013997:Time Factors
chemical_list: D003470:Culture Media; D012336:RNA, Ribosomal, 16S; D011933:Reagent Kits, Diagnostic
keywords: Blood culture; Conventional method; Identification; Isolates; MALDI-TOF MS