title: Effects of increase in fish oil intake on intestinal eicosanoids and inflammation in a mouse model of colitis.
abstract: BACKGROUND
  Inflammatory bowel diseases (IBD) are chronic intestinal inflammatory diseases affecting about 1% of western populations. New eating behaviors might contribute to the global emergence of IBD. Although the immunoregulatory effects of omega-3 fatty acids have been well characterized in vitro, their role in IBD is controversial.
  
  
  METHODS
  The aim of this study was to assess the impact of increased fish oil intake on colonic gene expression, eicosanoid metabolism and development of colitis in a mouse model of IBD. Rag-2 deficient mice were fed fish oil (FO) enriched in omega-3 fatty acids i.e. EPA and DHA or control diet for 4 weeks before colitis induction by adoptive transfer of naïve T cells and maintained in the same diet for 4 additional weeks. Onset of colitis was monitored by colonoscopy and further confirmed by immunological examinations. Whole genome expression profiling was made and eicosanoids were measured by HPLC-MS/MS in colonic samples.
  
  
  RESULTS
  A significant reduction of colonic proinflammatory eicosanoids in FO fed mice compared to control was observed. However, neither alteration of colonic gene expression signature nor reduction in IBD scores was observed under FO diet.
  
  
  CONCLUSIONS
  Thus, increased intake of dietary FO did not prevent experimental colitis.
authors.forename: Nabil
authors.initials: N
authors.lastname: Bosco
authors.forename: Viral
authors.initials: V
authors.lastname: Brahmbhatt
authors.forename: Manuel
authors.initials: M
authors.lastname: Oliveira
authors.forename: Francois-Pierre
authors.initials: FP
authors.lastname: Martin
authors.forename: Pia
authors.initials: P
authors.lastname: Lichti
authors.forename: Frederic
authors.initials: F
authors.lastname: Raymond
authors.forename: Robert
authors.initials: R
authors.lastname: Mansourian
authors.forename: Sylviane
authors.initials: S
authors.lastname: Metairon
authors.forename: Cecil
authors.initials: C
authors.lastname: Pace-Asciak
authors.forename: Viktoria
authors.initials: V
authors.lastname: Bastic Schmid
authors.forename: Serge
authors.initials: S
authors.lastname: Rezzi
authors.forename: Dirk
authors.initials: D
authors.lastname: Haller
authors.forename: Jalil
authors.initials: J
authors.lastname: Benyacoub
mesh_terms: D000818:Animals; D003092:Colitis / Q000178:diet therapy* / Q000235:genetics / Q000378:metabolism*; D003106:Colon / Q000503:physiopathology; D016207:Cytokines / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics; D004195:Disease Models, Animal; D015777:Eicosanoids / Q000378:metabolism*; D015525:Fatty Acids, Omega-3 / Q000494:pharmacology; D005395:Fish Oils / Q000737:chemistry / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects; D015212:Inflammatory Bowel Diseases / Q000209:etiology; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000187:drug effects*; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D006377:T-Lymphocytes, Helper-Inducer / Q000378:metabolism / Q000473:pathology
chemical_list: D016207:Cytokines; D004268:DNA-Binding Proteins; D015777:Eicosanoids; D015525:Fatty Acids, Omega-3; D005395:Fish Oils; C499308:Rag2 protein, mouse