title: Indole-porphyrin hybrids produced by metagenomics.
abstract: New indole-porphyrin hybrid molecules were isolated from Escherichia coli harboring metagenomic DNA from the Japanese marine sponge Discodermia calyx. The indole was appended to the reactive vinyl substituent of the harderoporphyrin chromophore, encoded by the insert DNA. Thus, the chimeric pathway between the heterologously expressed porphyrins and the endogenous indole generated new indole-conjugated chiral porphyrins in E. coli.
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Tokyo 113-0033, Japan.
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Yang
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Wakimoto
authors.forename: Yuya
authors.initials: Y
authors.lastname: Takeshige
authors.forename: Rui
authors.initials: R
authors.lastname: He
authors.forename: Yoko
authors.initials: Y
authors.lastname: Egami
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Awakawa
authors.forename: Ikuro
authors.initials: I
authors.lastname: Abe
mesh_terms: D000818:Animals; D004247:DNA / Q000235:genetics*; D004926:Escherichia coli / Q000737:chemistry; D007211:Indoles / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D054892:Metagenome / Q000235:genetics*; D056186:Metagenomics; D015394:Molecular Structure; D011161:Porifera / Q000235:genetics*; D011166:Porphyrins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D007211:Indoles; D011166:Porphyrins; D004247:DNA