title: Sesquiterpene coumarins from Ferula fukanensis and their pro-inflammatory cytokine gene expression inhibitory effects.
abstract: Six new sesquiterpene coumarin derivatives, fukanefuromarin H-M (1-6), were isolated from an 80% aqueous methanol extract of the roots of Ferula fukanensis. The structures were elucidated on the basis of spectroscopic evidence, particularly heteronuclear multiple-bond connectivity (HMBC) and high-resolution MS. The sesquiterpene coumarin derivatives inhibited nitric oxide (NO) and inducible NO synthase (iNOS), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) gene expression in a murine macrophage-like cell line (RAW264.7), which was activated by lipopolysaccharide (LPS) and recombinant mouse interferon-γ (IFN-γ).
authors.affiliation: School of Pharmacy, Nihon University, 7–7–1 Narashinodai, Funabashi, Chiba, Japan.
authors.forename: Tsunetake
authors.initials: T
authors.lastname: Motai
authors.forename: Akihiro
authors.initials: A
authors.lastname: Daikonya
authors.forename: Susumu
authors.initials: S
authors.lastname: Kitanaka
mesh_terms: D000818:Animals; D002460:Cell Line; D003374:Coumarins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D005309:Ferula / Q000737:chemistry*; D007371:Interferon-gamma / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000494:pharmacology; D012717:Sesquiterpenes / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: D003374:Coumarins; D015850:Interleukin-6; D011994:Recombinant Proteins; D012717:Sesquiterpenes; D014409:Tumor Necrosis Factor-alpha; D009569:Nitric Oxide; D007371:Interferon-gamma; D052247:Nitric Oxide Synthase Type II