title: ¹H, ¹³C, and ¹⁵N backbone resonance assignments of the 37 kDa voltage-gated Ca²⁺ channel β4 subunit core SH3-GK domains.
abstract: The β subunit of the voltage-gated Ca(2+) channel (α1, α2δ, and β subunits) is a member of the MAGUK family of proteins and plays an essential role in regulating Ca(2+) channel trafficking and gating. It also serves as a central interaction partner for various Ca(2+) channel regulatory proteins. We report here the nearly complete (1)H, (13)C, and (15)N backbone resonance assignments of the 37 kDa core SH3-GK domains of the β4 subunit. This is the first report of solution assignments for β subunits, and as such will lay the foundation for future investigations of interaction site mapping, functional dynamics, and protein complex structure determination.
authors.affiliation: Department of Clinical Sciences, College of Veterinary Medicine, Michigan State University, 736 Wilson Rd, Room D208, East Lansing, MI, 48824, USA.
authors.forename: Xingfu
authors.initials: X
authors.lastname: Xu
authors.forename: William A
authors.initials: WA
authors.lastname: Horne
mesh_terms: D000595:Amino Acid Sequence; D015220:Calcium Channels / Q000737:chemistry*; D002247:Carbon Isotopes; D006801:Humans; D006859:Hydrogen; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009587:Nitrogen Isotopes; D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D021122:Protein Subunits / Q000737:chemistry*
chemical_list: D015220:Calcium Channels; D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D021122:Protein Subunits; D006859:Hydrogen