title: Identification of putative markers of triclabendazole resistance by a genome-wide analysis of genetically recombinant Fasciola hepatica.
abstract: Despite years of investigation into triclabendazole (TCBZ) resistance in Fasciola hepatica, the genetic mechanisms responsible remain unknown. Extensive analysis of multiple triclabendazole-susceptible and -resistant isolates using a combination of experimental in vivo and in vitro approaches has been carried out, yet few, if any, genes have been demonstrated experimentally to be associated with resistance phenotypes in the field. In this review we summarize the current understanding of TCBZ resistance from the approaches employed to date. We report the current genomic and genetic resources for F. hepatica that are available to facilitate novel functional genomics and genetic experiments for this parasite in the future. Finally, we describe our own non-biased approach to mapping the major genetic loci involved in conferring TCBZ resistance in F. hepatica.
authors.affiliation: Veterinary Parasitology, Institute of Infection and Global Health, University of Liverpool, Liverpool, L3 5RF, UK. jhodgkin@liverpool.ac.uk
authors.forename: J
authors.initials: J
authors.lastname: Hodgkinson
authors.forename: K
authors.initials: K
authors.lastname: Cwiklinski
authors.forename: N J
authors.initials: NJ
authors.lastname: Beesley
authors.forename: S
authors.initials: S
authors.lastname: Paterson
authors.forename: D J L
authors.initials: DJ
authors.lastname: Williams
mesh_terms: D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D001562:Benzimidazoles / Q000494:pharmacology*; D002874:Chromosome Mapping; D004351:Drug Resistance / Q000235:genetics*; D005210:Fasciola hepatica / Q000187:drug effects / Q000235:genetics*; D005211:Fascioliasis / Q000469:parasitology*; D056426:Genetic Loci; D005819:Genetic Markers; D005828:Genetics, Population; D049751:Genome, Helminth / Q000235:genetics; D055106:Genome-Wide Association Study; D023281:Genomics; D006801:Humans; D012756:Sheep; D000077682:Triclabendazole
chemical_list: D000871:Anthelmintics; D001562:Benzimidazoles; D005819:Genetic Markers; D000077682:Triclabendazole