title: Conserved regions of ribonucleoprotein ribonuclease MRP are involved in interactions with its substrate.
abstract: Ribonuclease (RNase) MRP is a ubiquitous and essential site-specific eukaryotic endoribonuclease involved in the metabolism of a wide range of RNA molecules. RNase MRP is a ribonucleoprotein with a large catalytic RNA moiety that is closely related to the RNA component of RNase P, and multiple proteins, most of which are shared with RNase P. Here, we report the results of an ultraviolet-cross-linking analysis of interactions between a photoreactive RNase MRP substrate and the Saccharomyces cerevisiae RNase MRP holoenzyme. The results show that the substrate interacts with phylogenetically conserved RNA elements universally found in all enzymes of the RNase P/MRP family, as well as with a phylogenetically conserved RNA region that is unique to RNase MRP, and demonstrate that four RNase MRP protein components, all shared with RNase P, interact with the substrate. Implications for the structural organization of RNase MRP and the roles of its components are discussed.
authors.affiliation: Department of Biochemistry and Molecular Biology and Center for RNA Molecular Biology, Pennsylvania State University, University Park, PA 16802, USA.
authors.forename: Olga
authors.initials: O
authors.lastname: Esakova
authors.forename: Anna
authors.initials: A
authors.lastname: Perederina
authors.forename: Igor
authors.initials: I
authors.lastname: Berezin
authors.forename: Andrey S
authors.initials: AS
authors.lastname: Krasilnikov
mesh_terms: D004722:Endoribonucleases / Q000737:chemistry / Q000145:classification / Q000378:metabolism*; D020035:Holoenzymes / Q000378:metabolism; D008958:Models, Molecular; D012261:Ribonucleoproteins / Q000737:chemistry / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology
chemical_list: D020035:Holoenzymes; D012261:Ribonucleoproteins; D004722:Endoribonucleases