title: [The effects of RH 421 on the activity of amphotericin B in cell and model membranes].
abstract: The combined action of polyene macrolide antibiotic amphotericin B and styryl dye RH 421 in model lipid membranes and in yeast Saccharomyces cerevisiae has been studied. RH 421 insertion inti the ergosterol-containing lipid bilayers led to the increase of the steady-state number of open amphotericin channels by 8.5 +/- 3.2 times. Paper disk agar diffusion method has demonstrated that RH 421 enhanced the antimicrobial effect of amphotericin B leading to an increase in the zone of growth inhibition of S. cerevisiae strain by 1.5 times. Our data suggest that RH 421 is a potential synergist of amphotericin B and can be used in new formulations with improved pharmacological properties.
authors.forename: E V
authors.initials: EV
authors.lastname: Mikhaĭlova
authors.forename: S S
authors.initials: SS
authors.lastname: Efimova
authors.forename: O S
authors.initials: OS
authors.lastname: Ostroumova
mesh_terms: D000666:Amphotericin B / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004357:Drug Synergism; D004875:Ergosterol / Q000737:chemistry / Q000378:metabolism; D008051:Lipid Bilayers / Q000737:chemistry / Q000378:metabolism; D008563:Membrane Lipids / Q000737:chemistry / Q000378:metabolism; D008566:Membranes / Q000187:drug effects*; D011726:Pyridinium Compounds / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000254:growth & development; D013343:Styrenes / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D008051:Lipid Bilayers; D008563:Membrane Lipids; D011726:Pyridinium Compounds; D013343:Styrenes; C070534:RH 421; D000666:Amphotericin B; D004875:Ergosterol