title: High proportion of CD34+/CD38-cells is positively correlated with poor prognosis in newly diagnosed childhood acute lymphoblastic leukemia.
abstract: The CD34+/CD38- immunophenotype is used to identify candidate hematopoietic stem cells (HSCs) and leukemia-initiating cells (LICs). However, the clinical significance of the CD34+/CD38-proportion in childhood acute lymphoblastic leukemia (ALL) is not well established. Here, we found that a high proportion of CD34+/CD38- cells from 112 patients with childhood ALL was negatively correlated with the outcome. Also the percentage of CD34+/CD38- cells was associated with clinical and biological features of patients with ALL. Further, a high proportion of CD34+/CD38- cells in childhood ALL was positively correlated with advanced risk subgroups, which could predict risk stratification by receiver operating characteristic (ROC) curve analysis. In addition, a larger tumor burden and lower survival rate were observed in mice injected with CD34+/CD38- cells, but not in mice injected with other fractions. Our data reveal that a high proportion of CD34+/CD38- cells is positively associated with a poor prognosis of childhood ALL, to further guide therapy of the disease.
authors.affiliation: Center for Clinical Molecular Medicine.
authors.forename: Juan
authors.initials: J
authors.lastname: Long
authors.forename: Shan
authors.initials: S
authors.lastname: Liu
authors.forename: Kang
authors.initials: K
authors.lastname: Li
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhou
authors.forename: Penghui
authors.initials: P
authors.lastname: Zhang
authors.forename: Lin
authors.initials: L
authors.lastname: Zou
mesh_terms: D051997:ADP-ribosyl Cyclase 1 / Q000378:metabolism*; D000293:Adolescent; D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism*; D002648:Child; D002675:Child, Preschool; D004195:Disease Models, Animal; D005260:Female; D064593:Heterografts; D006801:Humans; D016130:Immunophenotyping; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D051379:Mice; D014411:Neoplastic Stem Cells / Q000378:metabolism; D010641:Phenotype; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000378:metabolism* / Q000401:mortality*; D011379:Prognosis
chemical_list: D018952:Antigens, CD34; D051997:ADP-ribosyl Cyclase 1