title: Orthogonal labeling of M13 minor capsid proteins with DNA to self-assemble end-to-end multiphage structures.
abstract: M13 bacteriophage has been used as a scaffold to organize materials for various applications. Building more complex multiphage devices requires precise control of interactions between the M13 capsid proteins. Toward this end, we engineered a loop structure onto the pIII capsid protein of M13 bacteriophage to enable sortase-mediated labeling reactions for C-terminal display. Combining this with N-terminal sortase-mediated labeling, we thus created a phage scaffold that can be labeled orthogonally on three capsid proteins: the body and both ends. We show that covalent attachment of different DNA oligonucleotides at the ends of the new phage structure enables formation of multiphage particles oriented in a specific order. These have potential as nanoscale scaffolds for multi-material devices.
authors.affiliation: The David H. Koch Institute for Integrative Cancer Research, ‡Department of Materials Science and Engineering, ∥Department of Biology, and ⊥Department of Biological Engineering, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139, United States.
authors.forename: Gaelen T
authors.initials: GT
authors.lastname: Hess
authors.forename: Carla P
authors.initials: CP
authors.lastname: Guimaraes
authors.forename: Eric
authors.initials: E
authors.lastname: Spooner
authors.forename: Hidde L
authors.initials: HL
authors.lastname: Ploegh
authors.forename: Angela M
authors.initials: AM
authors.lastname: Belcher
mesh_terms: D020816:Amino Acid Motifs; D017104:Bacteriophage M13 / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D036022:Capsid Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000378:metabolism; D004279:DNA, Viral / Q000378:metabolism*; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D009693:Nucleic Acid Hybridization / Q000379:methods; D061848:Optical Imaging; D015202:Protein Engineering
chemical_list: D036022:Capsid Proteins; D004279:DNA, Viral; D005456:Fluorescent Dyes; D003546:Cysteine Endopeptidases