title: Identification of differential expressed proteins and characterization their mRNA expression in thermally stressed Apostichopus japonicus.
abstract: In this study, we present a comparative proteomic analysis of the global protein expression changes in sea cucumber after 7 days exposure at 25°C. Using two-dimensional electrophoresis followed by MALDI-TOF MS/MS, 27 protein spots with significant differences in abundance were identified and characterized. The identified proteins belonged primarily to the following four functional categories: cytoskeletal, material and energy metabolism, calcium homeostasis and extracellular matrix. The mRNA expression levels of 7 differentially expressed proteins were further assessed by qRT-PCR. The expression levels of 6 genes, including collagen, ATP synthase, major yolk protein, ferritin, nectin and protein disulfide isomerase showed significant differences under thermal stress, and among them, only two genes-ATP synthase and major yolk protein-showed consistent levels of protein and mRNA expression. Our results offer insight into the complex changes in protein turnover during higher temperature exposure in sea cucumber.
authors.affiliation: School of Marine Sciences, Ningbo University, Ningbo, Zhejiang Province 315211, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.forename: Yali
authors.initials: Y
authors.lastname: Lu
authors.forename: Chenghua
authors.initials: C
authors.lastname: Li
authors.forename: Xiurong
authors.initials: X
authors.lastname: Su
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Wang
authors.forename: Chunhua
authors.initials: C
authors.lastname: Jin
authors.forename: Ye
authors.initials: Y
authors.lastname: Li
authors.forename: Taiwu
authors.initials: T
authors.lastname: Li
mesh_terms: D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D018869:Heat-Shock Response / Q000235:genetics*; D006358:Hot Temperature; D014176:Protein Biosynthesis / Q000235:genetics; D011506:Proteins / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics; D020543:Proteome / Q000032:analysis* / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D040901:Proteomics; D012333:RNA, Messenger / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D047528:Stichopus
chemical_list: D011506:Proteins; D020543:Proteome; D012333:RNA, Messenger
keywords: Apostichopus japonicus; Thermal stress; Two-dimensional gel electrophoresis