title: Sialic acid-dependent attachment of mucins from three mouse strains to Entamoeba histolytica.
abstract: Mouse strain-specific differences in the carbohydrate composition of intestinal mucins were hypothesized to account for strain-dependent susceptibility to Entamoeba histolytica. To test this hypothesis, intestinal mucins from susceptible and resistant inbred strains of mice were analyzed for their O-glycan content and for their ability to inhibit amoebic adherence to (GalNAc)12-27-HSA neo-glycoproteins. The results showed that the colorectal mucin O-glycan of susceptible CBA mice was lower in sialic acid content than that of resistant C57BL/6 and BALB/c mice. Mucins from CBA mice were more potent inhibitors of E. histolytica adherence to neo-glycoproteins than were mucins from C57BL/6 or BALB/c mice. Consistent with the role of terminal Gal/GalNAc as a receptor for amoebic adherence, sialidase treatment of C57BL/6 and BALB/c colorectal mucins increased their ability to inhibit E. histolytica adherence to the neo-glycoproteins. These results provide evidence of mouse strain-specific differences in the sialic acids content of mucin O-glycans. These dissimilarities likely contribute to the differential susceptibility of the three mouse strains to E. histolytica infection.
authors.affiliation: Department of Parasitology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan. katoken@nagasaki-u.ac.jp
authors.forename: Kentaro
authors.initials: K
authors.lastname: Kato
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Takegawa
authors.forename: Katherine S
authors.initials: KS
authors.lastname: Ralston
authors.forename: Carol A
authors.initials: CA
authors.lastname: Gilchrist
authors.forename: Shinjiro
authors.initials: S
authors.lastname: Hamano
authors.forename: William A
authors.initials: WA
authors.lastname: Petri
authors.forename: Yasuro
authors.initials: Y
authors.lastname: Shinohara
mesh_terms: D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D003106:Colon / Q000378:metabolism / Q000469:parasitology; D004748:Entamoeba histolytica / Q000378:metabolism* / Q000502:physiology; D054794:Glycomics / Q000379:methods; D006023:Glycoproteins / Q000378:metabolism; D006790:Host-Parasite Interactions / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008808:Mice, Inbred CBA; D009077:Mucins / Q000378:metabolism* / Q000494:pharmacology; D019158:N-Acetylneuraminic Acid / Q000378:metabolism*; D011134:Polysaccharides / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D012007:Rectum / Q000378:metabolism / Q000469:parasitology; D013045:Species Specificity
chemical_list: D006023:Glycoproteins; D009077:Mucins; D011134:Polysaccharides; D019158:N-Acetylneuraminic Acid