title: Method development and validation of six bile acids for regulated bioanalysis: improving selectivity and sensitivity.
abstract: BACKGROUND
  Quantification of bile acids using LC-MS has previously been very challenging on triple quadrupole MS systems due to the absence of a primary fragment ion for unconjugated bile acids.
  
  
  RESULTS
  A LC-high-resolution/accurate mass MS method for the analysis of six bile acids (cholic acid, chenodeoxycholic acid, taurocholic acid, deoxycholic acid, lithocholic acid and ursodeoxycholic acid) was developed and successfully validated. The method includes a single extraction and a single injection with all analytes separated using target-selected ion monitoring (SIM) mode in two periods with a resolution of 70,000 and 140,000, respectively.
  
  
  CONCLUSIONS
  This is the first LC-high-resolution/accurate mass assay fully validated to quantify six bile acids for regulated bioanalysis.
authors.affiliation: Tandem Labs, Salt Lake City, UT, USA.
authors.forename: Troy
authors.initials: T
authors.lastname: Voelker
authors.forename: Hongxia
authors.initials: H
authors.lastname: Wang
authors.forename: Mitzi
authors.initials: M
authors.lastname: Irish
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.forename: Stephanie
authors.initials: S
authors.lastname: Harrison
authors.forename: Scott
authors.initials: S
authors.lastname: Reuschel
authors.forename: Patrick
authors.initials: P
authors.lastname: Bennett
authors.forename: Min
authors.initials: M
authors.lastname: Meng
mesh_terms: D001647:Bile Acids and Salts / Q000032:analysis*; D015415:Biomarkers / Q000097:blood*; D001798:Blood Proteins / Q000032:analysis*; D002635:Chenodeoxycholic Acid / Q000032:analysis; D019826:Cholic Acid / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods*; D003840:Deoxycholic Acid / Q000032:analysis; D006801:Humans; D008095:Lithocholic Acid / Q000032:analysis; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013656:Taurocholic Acid / Q000032:analysis; D014580:Ursodeoxycholic Acid / Q000032:analysis; D054928:Validation Studies as Topic
chemical_list: D001647:Bile Acids and Salts; D015415:Biomarkers; D001798:Blood Proteins; D003840:Deoxycholic Acid; D002635:Chenodeoxycholic Acid; D013656:Taurocholic Acid; D008095:Lithocholic Acid; D014580:Ursodeoxycholic Acid; D019826:Cholic Acid