title: Burkholderia sprentiae sp. nov., isolated from Lebeckia ambigua root nodules.
abstract: Seven Gram-stain-negative, rod-shaped bacteria were isolated from Lebeckia ambigua root nodules and authenticated on this host. Based on the 16S rRNA gene phylogeny, they were shown to belong to the genus Burkholderia, with the representative strain WSM5005(T) being most closely related to Burkholderia tuberum (98.08 % sequence similarity). Additionally, these strains formed a distinct group in phylogenetic trees based on the housekeeping genes gyrB and recA. Chemotaxonomic data including fatty acid profiles and analysis of respiratory quinones supported the assignment of the strains to the genus Burkholderia. Results of DNA-DNA hybridizations, and physiological and biochemical tests allowed genotypic and phenotypic differentiation of our strains from the closest species of the genus Burkholderia with a validly published name. Therefore, these strains represent a novel species for which the name Burkholderia sprentiae sp. nov. (type strain WSM5005(T) = LMG 27175(T) = HAMBI 3357(T)) is proposed.
authors.affiliation: Centre for Rhizobium Studies, Murdoch University, Western Australia 6150, Australia.
authors.forename: Sofie E
authors.initials: SE
authors.lastname: De Meyer
authors.affiliation: Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
authors.forename: Margo
authors.initials: M
authors.lastname: Cnockaert
authors.affiliation: Centre for Rhizobium Studies, Murdoch University, Western Australia 6150, Australia.
authors.forename: Julie K
authors.initials: JK
authors.lastname: Ardley
authors.affiliation: Separation Science and Metabolomics Laboratory, Murdoch University, Murdoch, Western Australia 6150, Australia.
authors.forename: Garth
authors.initials: G
authors.lastname: Maker
authors.affiliation: Department of Agriculture Western Australia, Baron Hay Court, South Perth, WA 6151, Australia.|Centre for Rhizobium Studies, Murdoch University, Western Australia 6150, Australia.
authors.forename: Ron
authors.initials: R
authors.lastname: Yates
authors.affiliation: Centre for Rhizobium Studies, Murdoch University, Western Australia 6150, Australia.
authors.forename: John G
authors.initials: JG
authors.lastname: Howieson
authors.affiliation: Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
authors.forename: Peter
authors.initials: P
authors.lastname: Vandamme
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D019117:Burkholderia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D007887:Fabaceae / Q000382:microbiology*; D005227:Fatty Acids / Q000737:chemistry; D005838:Genotype; D016013:Likelihood Functions; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000235:genetics; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D053204:Root Nodules, Plant / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013019:South Africa; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030778:ubiquinone 8