title: Metabolic engineering of Torulopsis glabrata for malate production.
abstract: The yeast Torulopsis glabrata CCTCC M202019, which is used for industrial pyruvate production, was chosen to explore the suitability of engineering this multi-vitamin auxotrophic yeast for increased malate production. Various metabolic engineering strategies were used to manipulate carbon flux from pyruvate to malate: (i) overexpression of pyruvate carboxylase and malate dehydrogenase; (ii) identification of the bottleneck in malate production by model iNX804; (iii) simultaneous overexpression of genes RoPYC, RoMDH and SpMAE1. Using these strategies, 8.5gL(-1) malate was accumulated in the engineered strain T.G-PMS, which was about 10-fold greater than that of the control strain T.G-26. The results presented here suggest that T. glabrata CCTCC M202019 is a promising candidate for industrial malate production.
authors.affiliation: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; Laboratory of Food Microbial-Manufacturing Engineering, Jiangnan University, Wuxi 214122, China.
authors.forename: Xiulai
authors.initials: X
authors.lastname: Chen
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Xu
authors.forename: Nan
authors.initials: N
authors.lastname: Xu
authors.forename: Wei
authors.initials: W
authors.lastname: Zou
authors.forename: Pan
authors.initials: P
authors.lastname: Zhu
authors.forename: Liming
authors.initials: L
authors.lastname: Liu
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
mesh_terms: D041221:Candida glabrata / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics; D008291:Malate Dehydrogenase / Q000096:biosynthesis / Q000235:genetics; D008293:Malates / Q000378:metabolism*; D060847:Metabolic Engineering; D011766:Pyruvate Carboxylase / Q000096:biosynthesis / Q000235:genetics; D019289:Pyruvic Acid / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D008293:Malates; C030298:malic acid; D019289:Pyruvic Acid; D008291:Malate Dehydrogenase; D011766:Pyruvate Carboxylase
keywords: Genome-scale metabolic model; Malate; Metabolic engineering; Torulopsis glabrata