title: Bacterial growth kinetics under a novel flexible methacrylate dressing serving as a drug delivery vehicle for antiseptics.
abstract: A flexible methacrylate powder dressing (Altrazeal®) transforms into a wound contour conforming matrix once in contact with wound exudate. We hypothesised that it may also serve as a drug delivery vehicle for antiseptics. The antimicrobial efficacy and influence on bacterial growth kinetics in combination with three antiseptics was investigated in an in vitro porcine wound model. Standardized in vitro wounds were contaminated with Staphylococcus aureus (MRSA; ATCC 33591) and divided into six groups: no dressing (negative control), methacrylate dressing alone, and combinations with application of 0.02% Polyhexamethylene Biguanide (PHMB), 0.4% PHMB, 0.1% PHMB + 0.1% betaine, 7.7 mg/mL Povidone-iodine (PVP-iodine), and 0.1% Octenidine-dihydrochloride (OCT) + 2% phenoxyethanol. Bacterial load per gram tissue was measured over five days. The highest reduction was observed with PVP-iodine at 24 h to log10 1.43 cfu/g, followed by OCT at 48 h to log10 2.41 cfu/g. Whilst 0.02% PHMB resulted in a stable bacterial load over 120 h to log10 4.00 cfu/g over 120 h, 0.1% PHMB + 0.1% betaine inhibited growth during the first 48 h, with slightly increasing bacterial numbers up to log10 5.38 cfu/g at 120 h. These results indicate that this flexible methacrylate dressing can be loaded with various antiseptics serving as drug delivery system. Depending on the selected combination, an individually shaped and controlled antibacterial effect may be achieved using the same type of wound dressing.
authors.affiliation: Department of Medicine I, Division of Infectious Diseases and Tropical Medicine, Medical University of Vienna, Waehringer Guertel 18-20, 1090 Vienna, Austria. ojan.assadian@meduniwien.ac.at.
authors.forename: Christina
authors.initials: C
authors.lastname: Forstner
authors.forename: Johannes
authors.initials: J
authors.lastname: Leitgeb
authors.forename: Rupert
authors.initials: R
authors.lastname: Schuster
authors.forename: Verena
authors.initials: V
authors.lastname: Dosch
authors.forename: Axel
authors.initials: A
authors.lastname: Kramer
authors.forename: Keith F
authors.initials: KF
authors.lastname: Cutting
authors.forename: David J
authors.initials: DJ
authors.lastname: Leaper
authors.forename: Ojan
authors.initials: O
authors.lastname: Assadian
mesh_terms: D000818:Animals; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage / Q000494:pharmacology*; D001458:Bandages; D001622:Betaine / Q000008:administration & dosage / Q000494:pharmacology; D001645:Biguanides / Q000494:pharmacology*; D004202:Disinfectants / Q000494:pharmacology; D016503:Drug Delivery Systems; D004357:Drug Synergism; D005026:Ethylene Glycols / Q000008:administration & dosage / Q000494:pharmacology; D007097:Imines; D007700:Kinetics; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects* / Q000254:growth & development; D011206:Povidone-Iodine / Q000008:administration & dosage / Q000494:pharmacology; D011725:Pyridines / Q000008:administration & dosage / Q000494:pharmacology; D013552:Swine; D013997:Time Factors; D014945:Wound Healing / Q000187:drug effects; D014947:Wounds and Injuries / Q000382:microbiology
chemical_list: D000891:Anti-Infective Agents, Local; D001645:Biguanides; D004202:Disinfectants; D005026:Ethylene Glycols; D007097:Imines; D011725:Pyridines; C031233:polihexanide; D001622:Betaine; D011206:Povidone-Iodine; C005398:phenoxyethanol; C034213:octenidine