title: Expression, purification, crystallization and preliminary X-ray diffraction analysis of Bifidobacterium adolescentis xylose isomerase.
abstract: Xylose isomerase (EC 5.3.1.5) is a key enzyme in xylose metabolism which is industrially important for the transformation of glucose and xylose into fructose and xylulose, respectively. The Bifidobacterium adolescentis xylA gene (NC_008618.1) encoding xylose isomerase (XI) was cloned and the enzyme was overexpressed in Escherichia coli. Purified recombinant XI was crystallized using the sitting-drop vapour-diffusion method with polyethylene glycol 3350 as the precipitating agent. A complete native data set was collected to 1.7 Å resolution using a synchrotron-radiation source. The crystals belonged to the orthorhombic space group P21212, with unit-cell parameters a = 88.78, b = 123.98, c = 78.63 Å.
authors.affiliation: Instituto de Física de São Carlos, Universidade de São Paulo, Trabalhador São Carlense 400, 13566-590 São Carlos-SP, Brazil.
authors.forename: Caio Vinicius
authors.initials: CV
authors.lastname: Dos Reis
authors.forename: Amanda
authors.initials: A
authors.lastname: Bernardes
authors.forename: Igor
authors.initials: I
authors.lastname: Polikarpov
mesh_terms: D019747:Aldose-Ketose Isomerases / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D001644:Bifidobacterium / Q000201:enzymology* / Q000235:genetics; D003460:Crystallization; D015964:Gene Expression Regulation, Bacterial; D014961:X-Ray Diffraction
chemical_list: D001426:Bacterial Proteins; D019747:Aldose-Ketose Isomerases; C023298:xylose isomerase
keywords: Bifidobacterium adolescentis; bioethanol; xylose isomerases