title: [Survival of calbindin, calretinin and parvalbumin positive neurons in mouse hippocampal CA area at chronic stage of pilocarpine-induced epilepsy].
abstract: OBJECTIVE
  To analyze the survival and the changes of proportions of Calbindin, Calretinin and Parvalbumin positive neurons in mouse hippocampal CA area at chronic stage of Pilocarpine-induced epilepsy.
  
  
  METHODS
  Calbindin, Calretinin and Parvalbumin immunofluoresence staining were done 2 months after Pilocarpine-induced epilepsy in mice or saline injection.
  
  
  RESULTS
  Two months after Pilocarine-induced epilepsy, the number of Calbindin, Calretinin and Parvalbumin positive neurons in the CA area decreased significantly compared with the control (P<0.01), especially the Calbindin positive neurons had a great drop and Pavalbumin positive neurons had a least drop. At the chronic stage of epilepsy, the proportion of Calbindin, Calretinin and Parvalbumin positive neurons in the CA area was changed. The content of Pavalbumin positive neurons increased whereas the content of Calbindin positive neurons decreased significantly compared with the control (P<0.01).
  
  
  CONCLUSIONS
  The changes of proportions of Calbindin, Calretinin and Parvalbumin positive neurons in the CA area of mouse hippocampus may be a factor in the ongoing epileptic activity at chronic stage of Pilocarpine-induced epilepsy.
authors.affiliation: Institute of Neurobiology, School of Medicine, Xi'an Jiaotong University; Environment and Genes Related to Diseases Key Laboratory of Education Ministry, Xi'an 710061, China.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Liu
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Fengru
authors.initials: F
authors.lastname: Tang
mesh_terms: D000818:Animals; D064032:Calbindin 2 / Q000378:metabolism*; D064026:Calbindins / Q000378:metabolism*; D002470:Cell Survival / Q000502:physiology; D002908:Chronic Disease; D004827:Epilepsy / Q000139:chemically induced / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D051379:Mice; D009474:Neurons / Q000378:metabolism; D010320:Parvalbumins / Q000378:metabolism*; D010862:Pilocarpine; D005680:gamma-Aminobutyric Acid / Q000378:metabolism
chemical_list: D064032:Calbindin 2; D064026:Calbindins; D010320:Parvalbumins; D010862:Pilocarpine; D005680:gamma-Aminobutyric Acid