title: Expression of Ceratophyllum demersum phytochelatin synthase, CdPCS1, in Escherichia coli and Arabidopsis enhances heavy metal(loid)s accumulation.
abstract: Phytochelatin synthase (PCS) gene encoding key enzyme for heavy metal detoxification and accumulation has been characterised from different sources and used to develop a technology for bioremediation. Past efforts provided limited success and contradictory results. Therefore, functional characterisation of PCS gene from new sources into different target systems is considered as an important task in the area of bioremediation. Earlier, we isolated and functionally characterised PCS gene from an aquatic macrophyte Ceratophyllum demersum L., a metal accumulator aquatic plant. Expression of this gene, CdPCS1, in tobacco enhanced PC synthesis and metal accumulation of transgenic tobacco plants. In the present study, we have expressed CdPCS1 in more diverse systems, Escherichia coli and Arabidopsis, and studied growth and metal accumulation of transgenic organisms. The expression of CdPCS1 in E. coli offered tolerance against cadmium as well as higher accumulation accompanied with PCS1 activity. The expression of CdPCS1 in Arabidopsis showed a significant enhanced accumulation of heavy metal(loid)s in aerial parts without significant difference in growth parameters in comparison to wild-type Arabidopsis plants. Our study suggests that CdPCS1 can be utilised for enhancing bioremediation potential of different organisms using biotechnological approaches.
authors.affiliation: National Botanical Research Institute (NBRI), Council of Scientific and Industrial Research (CSIR), Rana Pratap Marg, Lucknow, 226001, India.
authors.forename: Devesh
authors.initials: D
authors.lastname: Shukla
authors.forename: Ravi
authors.initials: R
authors.lastname: Kesari
authors.forename: Manish
authors.initials: M
authors.lastname: Tiwari
authors.forename: Sanjay
authors.initials: S
authors.lastname: Dwivedi
authors.forename: Rudra Deo
authors.initials: RD
authors.lastname: Tripathi
authors.forename: Pravendra
authors.initials: P
authors.lastname: Nath
authors.forename: Prabodh Kumar
authors.initials: PK
authors.lastname: Trivedi
mesh_terms: D000222:Adaptation, Physiological / Q000187:drug effects; D019881:Aminoacyltransferases / Q000378:metabolism*; D017360:Arabidopsis / Q000235:genetics*; D002104:Cadmium / Q000633:toxicity; D019669:Embryophyta / Q000187:drug effects / Q000201:enzymology*; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D019216:Metals, Heavy / Q000633:toxicity*; D030821:Plants, Genetically Modified; D036226:Seedlings / Q000187:drug effects / Q000254:growth & development; D013312:Stress, Physiological / Q000187:drug effects
chemical_list: D019216:Metals, Heavy; D002104:Cadmium; D019881:Aminoacyltransferases; C093784:glutathione gamma-glutamylcysteinyltransferase