title: Crystallization and preliminary X-ray diffraction analysis of the DNA-binding domain of the response regulator SaeR from Staphylococcus epidermidis.
abstract: SaeR is the response regulator of the SaeRS two-component signal transduction system, which is involved in regulating bacterial autolysis and biofilm formation. SaeR comprises an N-terminal receiver domain and a C-terminal effector domain. The effector domain possesses DNA-binding and transactivation functions. Here, the effector domain of SaeR from Staphylococcus epidermidis was purified and crystallized using the sitting-drop vapour-diffusion method. The crystals diffracted to a resolution of 2.15 Å and belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 34.20, b = 53.78, c = 111.66 Å. Determining the structure will provide insights into the mechanisms underlying DNA binding.
authors.affiliation: Department of Biotechnology, Hungkuang University, Taichung 433, Taiwan.
authors.forename: Sheng Chia
authors.initials: SC
authors.lastname: Chen
authors.forename: Chi Hung
authors.initials: CH
authors.lastname: Huang
authors.forename: Yu Ren
authors.initials: YR
authors.lastname: Chen
authors.forename: Chia Shin
authors.initials: CS
authors.lastname: Yang
authors.forename: Ching Ting
authors.initials: CT
authors.lastname: Lin
authors.forename: Yeh
authors.initials: Y
authors.lastname: Chen
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism; D003460:Crystallization; D004269:DNA, Bacterial / Q000737:chemistry* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding / Q000502:physiology; D017434:Protein Structure, Tertiary; D013212:Staphylococcus epidermidis; D014157:Transcription Factors; D014961:X-Ray Diffraction
chemical_list: D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004268:DNA-Binding Proteins; C433805:SaeR protein, Staphylococcus aureus; D014157:Transcription Factors
keywords: SaeR; Staphylococcus epidermidis; two-component systems