title: Genetic characterization of novel putative rhabdovirus and dsRNA virus from Japanese persimmon.
abstract: Deep-sequencing analysis of nucleic acids from leaf tissue of Japanese persimmon trees exhibiting fruit apex disorder in some fruits detected two molecules that were graft transmitted to healthy seedlings. One of the complete genomes consisted of 13 467 nt and encoded six genes similar to those of plant rhabdoviruses. The virus formed a distinct cluster in the genus Cytorhabdovirus with lettuce necrotic yellows virus, lettuce yellow mottle virus and strawberry crinkle virus in a phylogenetic tree based on the L protein (RNA-dependent RNA polymerase, RdRp). The other consisted of 7475 nt and shared a genome organization similar to those of some insect and fungal viruses having dsRNA genomes. In a phylogenetic tree using the RdRp sequence of several unassigned dsRNA viruses, the virus formed a possible new genus cluster with two insect viruses, Circulifer tenellus virus 1 and Spissistilus festinus virus 1, and one plant virus, cucurbit yellows-associated virus.
authors.affiliation: Grape and Persimmon Research Station, National Institute of Fruit Tree Science (NIFTS), National Agriculture and Food Research Organization (NARO), Akitsu, Hiroshima 739-2494, Japan.
authors.forename: Takao
authors.initials: T
authors.lastname: Ito
authors.affiliation: Grape and Persimmon Research Station, National Institute of Fruit Tree Science (NIFTS), National Agriculture and Food Research Organization (NARO), Akitsu, Hiroshima 739-2494, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Suzaki
authors.affiliation: Grape and Persimmon Research Station, National Institute of Fruit Tree Science (NIFTS), National Agriculture and Food Research Organization (NARO), Akitsu, Hiroshima 739-2494, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Nakano
mesh_terms: D016000:Cluster Analysis; D029788:Diospyros / Q000821:virology*; D059014:High-Throughput Nucleotide Sequencing; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D018515:Plant Leaves / Q000821:virology; D012328:RNA Viruses / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D012367:RNA, Viral / Q000235:genetics
chemical_list: D012367:RNA, Viral