title: Applications of high-resolution MS in bioanalysis.
abstract: High-resolution MS (HRMS) in conjunction with LC (LC-HRMS) has become available to many laboratories in the pharmaceutical industry. Due to its enhanced, though sometime perceived, specificity using the high-resolution power and its capability of simultaneous quantitation and structural elucidation using the post-acquisition data mining feature, utilization of LC-HRMS for bioanalysis could lead to potential rapid and reliable method development as well as sample analysis, thus generating both cost and resource savings. Here, we would like to share our perspectives about several current and future applications of LC-HRMS in bioanalysis. We will also discuss the factors influencing the quality of method establishment and potential pitfalls that need to be considered for the utilization of LC-HRMS in the field of regulated bioanalysis. We believe when utilized appropriately, LC-HRMS will play a significant role in the future landscape of quantitative bioanalysis.
authors.affiliation: Janssen Research & Development, LLC, Pharmaceutical Companies of Johnson & Johnson, 1000 Route 202 South, Raritan, NJ 08869, USA. qhuang5@its.jnj.com
authors.forename: Mike-Qingtao
authors.initials: MQ
authors.lastname: Huang
authors.forename: Zhongping John
authors.initials: ZJ
authors.lastname: Lin
authors.forename: Naidong
authors.initials: N
authors.lastname: Weng
mesh_terms: D015415:Biomarkers / Q000032:analysis*; D057166:High-Throughput Screening Assays; D006801:Humans; D013058:Mass Spectrometry; D040901:Proteomics
chemical_list: D015415:Biomarkers