title: Simplified serum neutralization test based on enhanced green fluorescent protein-tagged classical swine fever virus.
abstract: The neutralization immunofluorescence test (NIFT), currently used for detecting neutralizing antibodies (NAbs) against classical swine fever virus (CSFV), is time-consuming. Here, a simplified neutralization test based on enhanced green fluorescent protein (EGFP)-tagged CSFV (EGFP-NT) was developed for direct detection of anti-CSFV NAbs without immunostaining. The relative sensitivity and specificity between EGFP-NT and NIFT or blocking enzyme-linked immunosorbent assay (ELISA) were both 100%. The NAb titers by EGFP-NT and the blocking rates by blocking ELISA showed a good correlation.
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
authors.forename: Yongfeng
authors.initials: Y
authors.lastname: Li
authors.forename: Liang
authors.initials: L
authors.lastname: Shen
authors.forename: Yuan
authors.initials: Y
authors.lastname: Sun
authors.forename: Jin
authors.initials: J
authors.lastname: Yuan
authors.forename: Junhua
authors.initials: J
authors.lastname: Huang
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.forename: Su
authors.initials: S
authors.lastname: Li
authors.forename: Yuzi
authors.initials: Y
authors.lastname: Luo
authors.forename: Hua-Ji
authors.initials: HJ
authors.lastname: Qiu
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood*; D000914:Antibodies, Viral / Q000097:blood*; D006692:Classical Swine Fever Virus / Q000276:immunology*; D005455:Fluorescent Antibody Technique; D049452:Green Fluorescent Proteins / Q000032:analysis*; D009500:Neutralization Tests / Q000379:methods*; D012680:Sensitivity and Specificity; D044967:Serum / Q000276:immunology*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins