title: Spectrometric method for the assay of ramipril using molybdophosphoric acid.
abstract: Ramipril is a drug of the angiotensin converting enzyme inhibitor class.
  
  
  OBJECTIVE
  A new molecular absorbance spectrometric method was developed for the assay of ramipril using molybdophosphoric acid in acidic medium.
  
  
  METHODS
  The reaction product showed a maximum absorbance at 361 nm. The optimum conditions of the reaction were established. The developed method was validated.
  
  
  RESULTS
  The method showed a good linearity in the range of 8 - 36 microg/ml (correlation coefficient r = 0.9996). The detection limit (LD) was 0.86microg/ml and the quantification limit (LQ) 2.88 pg/ml. Precision and accuracy were determined (RSD = 1.15%); mean recovery was 98.90% in the 97.13-101.03% concentration range.
  
  
  CONCLUSIONS
  The proposed method is simple, easy to perform, sensitive, linear, precise, accurate and robust.
authors.affiliation: University of Medicine and Pharmacy Grigore T Popa lasi, Faculty of Pharmacy.
authors.forename: Mădălina
authors.initials: M
authors.lastname: Vieriu
authors.forename: Nela
authors.initials: N
authors.lastname: Bibire
authors.forename: Gladiola
authors.initials: G
authors.lastname: Tântaru
authors.forename: M
authors.initials: M
authors.lastname: Apostu
authors.forename: Alina Diana
authors.initials: AD
authors.lastname: Panainte
authors.forename: V
authors.initials: V
authors.lastname: Dorneanu
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000032:analysis / Q000737:chemistry*; D007202:Indicators and Reagents / Q000737:chemistry*; D057230:Limit of Detection; D008982:Molybdenum / Q000737:chemistry*; D010756:Phosphoric Acids / Q000737:chemistry*; D017257:Ramipril / Q000032:analysis / Q000737:chemistry*; D015203:Reproducibility of Results; D013053:Spectrophotometry / Q000379:methods*
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D007202:Indicators and Reagents; D010756:Phosphoric Acids; D008982:Molybdenum; D017257:Ramipril; C003125:phosphomolybdic acid