title: Pharmacokinetics, urinary excretion and plasma protein binding of ofloxacin in water buffalo calves (Bubalus bubalis).
abstract: Pharmacokinetics and urinary excretion of an intravenous dose of 5 mg.kg-1 ofloxacin were investigated in water buffalo calves. Plasma concentrations of ofloxacin were determined by high-performance liquid chromatography. Ofloxacin was rapidly distributed from the central to the peripheral compartment as evidenced by a short distribution half-life (0.09 h ± 0.003 h) and high K12 (4.7 h(-1) ± 0.1 h(-1)), and was detected in plasma for 8 h. The large volume of distribution (2.48 L.kg(-1) ± 0.18 L.kg(-1)) obtained in this study indicated high distribution of ofloxacin in water buffalo calves. The elimination half-life, the area under the plasma drug concentration-time curve and total body clearance were 2.11 h ± 0.13 h, 6.20 µg.mL(-1) ± 0.23 µg.mL(-1).h and 0.81 mL.kg(-1).h(-1) ± 0.03 mL.kg(-1).h(-1), respectively. About 18.7% of administered drug was bound to plasma proteins and approximately 32.5% of the administered dose was recovered in urine within 48 h. The results of the study indicated a favourable pharmacokinetic profile of ofloxacin in water buffalo calves, which suggests that ofloxacin may be effective against urinary pathogens in this species.
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India.
authors.forename: Ajay K
authors.initials: AK
authors.lastname: Ola
authors.forename: Harpal S
authors.initials: HS
authors.lastname: Sandhu
authors.forename: Vinod K
authors.initials: VK
authors.lastname: Dumka
authors.forename: Bibhuti
authors.initials: B
authors.lastname: Ranjan
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D019540:Area Under Curve; D001798:Blood Proteins; D002020:Buffaloes / Q000097:blood* / Q000652:urine; D006207:Half-Life; D015242:Ofloxacin / Q000097:blood / Q000493:pharmacokinetics* / Q000652:urine; D011485:Protein Binding
chemical_list: D000900:Anti-Bacterial Agents; D001798:Blood Proteins; D015242:Ofloxacin