title: Binding of white spot syndrome virus to Artemia sp. cell membranes.
abstract: Using differential velocity centrifugation, cell membranes of Artemia sp. were prepared, and their binding to white spot syndrome virus (WSSV) was analyzed in vitro. The results indicated that WSSV can specifically bind to Artemia cell membranes, and that WSSV receptor very likely existed in this membrane, which suggested that Artemia sp. may be a reservoir of WSSV. This study investigated the specific WSSV binding site by performing competitive inhibition experiments using shrimp gill cell membranes to bind WSSV to Artemia cell membranes. The results showed that shrimp gill cell membranes had a distinct inhibition effect on the specific binding of Artemia cell membranes to WSSV. Thus, potentially similar WSSV receptors or binding sites existed on Artemia sp. cell membranes and shrimp gill cell membranes. Taken together, these findings may provide experimental basis for the development of an effective approach to controlling WSSV, and theoretical basis for the study of WSSV receptors.
authors.affiliation: College of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang, Henan 471003, China.
authors.forename: Shuying
authors.initials: S
authors.lastname: Feng
authors.forename: Guangda
authors.initials: G
authors.lastname: Li
authors.forename: Wenpo
authors.initials: W
authors.lastname: Feng
authors.forename: Jie
authors.initials: J
authors.lastname: Huang
mesh_terms: D000818:Animals; D001156:Artemia / Q000821:virology*; D002462:Cell Membrane / Q000821:virology*; D011991:Receptors, Virus / Q000378:metabolism; D053585:Virus Attachment; D046848:White spot syndrome virus 1 / Q000502:physiology*
chemical_list: D011991:Receptors, Virus
keywords: Artemia cell membranes; BSA; Binding; DIG; Host; PTA; RT; Receptor; WSSV; bovine serum albumin; digoxigenin; phosphotungstate; room temperature