title: Deregulated expression of miR-145 in manifold human cancer cells.
abstract: MicroRNAs play important roles in the processes of tumor initiation and progression. The expression level of miR-145 in gastric, liver, and cervical cancers has been rarely investigated. Whether miR-145 may function as a common tumor suppressor in the generation of tumor phenotype needs to be clarified. miR-145 expression was determined by RT-qPCR in various human cancer tissues including those of gastric, liver, colon, and cervical cancers. Cancer cell lines were transfected with miR-145 precursor, anti-miR-145 inhibitor, or negative control, and cells' proliferation, migration, and invasion activities were analyzed. The gene target of miR-145 was confirmed by luciferase assay and Western blot. The miR-145 expression level was lower by 37.68-, 2.64-, 2.69- and 2.39-fold in gastric, liver, colon, and cervical cancer tissues, respectively, compared to corresponding nontumorous controls. Moreover, miR-145 levels were significantly downregulated in various cancer cell lines. We further demonstrated that miR-145 could suppress anchorage-independent growth and cell motility in both the liver cancer cell line Hep-G2 and the gastric cancer cell line MKN-45, and inhibited cell proliferation in a cell type-specific manner. Insulin receptor substrate-1 (IRS1) was identified as a target gene of miR-145, by which miR-145 was able to suppress cell proliferation. miR-145 suppresses cell proliferation, anchorage-independent growth, cell motility, and may serve as a tumor suppressor.
authors.affiliation: Department of Pathology, School of Medicine, Shandong University, Jinan, PR China.
authors.forename: Ai-Yan
authors.initials: AY
authors.lastname: Xing
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Duan-Bo
authors.initials: DB
authors.lastname: Shi
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: Zhang
authors.forename: Chao
authors.initials: C
authors.lastname: Gao
authors.forename: Xiu-Quan
authors.initials: XQ
authors.lastname: He
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Liu
authors.forename: Peng
authors.initials: P
authors.lastname: Gao
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D003110:Colonic Neoplasms / Q000235:genetics; D015536:Down-Regulation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006801:Humans; D055504:Insulin Receptor Substrate Proteins / Q000235:genetics / Q000378:metabolism; D008113:Liver Neoplasms / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics*; D012016:Reference Values; D013274:Stomach Neoplasms / Q000235:genetics; D014410:Tumor Stem Cell Assay; D002583:Uterine Cervical Neoplasms / Q000235:genetics
chemical_list: C526292:IRS1 protein, human; D055504:Insulin Receptor Substrate Proteins; C541010:MIRN145 microRNA, human; D035683:MicroRNAs
keywords: Cell motility; Cell proliferation; Tumor; miR-145