title: Release kinetics of platelet-derived and plasma-derived growth factors from autologous plasma rich in growth factors.
abstract: Many studies have evaluated the biological effects of platelet rich plasma reporting the final outcomes on cell and tissues. However, few studies have dealt with the kinetics of growth factor delivery by plasma rich in growth factors. Venous blood was obtained from three healthy volunteers and processed with PRGF-Endoret technology to prepare autologous plasma rich in growth factors. The gel-like fibrin scaffolds were then incubated in triplicate, in a cell culture medium to monitor the release of PDGF-AB, VEGF, HGF and IGF-I during 8 days of incubation. A leukocyte-platelet rich plasma was prepared employing the same technology and the concentrations of growth factors and interleukin-1β were determined after 24h of incubation. After each period, the medium was collected, fibrin clot was destroyed and the supernatants were stored at -80°C until analysis. The growth factor delivery is diffusion controlled with a rapid initial release by 30% of the bioactive content after 1h of incubation and a steady state release when almost 70% of the growth factor content has been delivered. Autologous fibrin matrix retained almost 30% of the amount of the growth factors after 8 days of incubation. The addition of leukocytes to the formula of platelet rich plasma did not increase the concentration of the growth factors, while it drastically increased the presence of pro-inflammatory IL-1β. Further studies employing an in vitro inflammatory model would be interesting to study the difference in growth factors and pro-inflammatory cytokines between leukocyte-free and leukocyte-rich platelet rich plasma.
authors.affiliation: Eduardo Anitua Foundation, c/Jose Maria Cagigal 19, 01007 Vitoria, Spain; BTI Biotechnology Institute, c/Jacinto Quincoces 39, 01007 Vitoria, Spain. Electronic address: eduardoanitua@eduardoanitua.com.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Anitua
authors.forename: Mari Mar
authors.initials: MM
authors.lastname: Zalduendo
authors.forename: Mohammad Hamdan
authors.initials: MH
authors.lastname: Alkhraisat
authors.forename: Gorka
authors.initials: G
authors.lastname: Orive
mesh_terms: D000465:Algorithms; D001792:Blood Platelets / Q000648:ultrastructure; D016207:Cytokines / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005337:Fibrin / Q000378:metabolism; D017228:Hepatocyte Growth Factor / Q000378:metabolism; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D053583:Interleukin-1beta / Q000378:metabolism; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D010949:Plasma / Q000737:chemistry / Q000378:metabolism*; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D016207:Cytokines; D036341:Intercellular Signaling Peptides and Proteins; D053583:Interleukin-1beta; D010982:Platelet-Derived Growth Factor; D042461:Vascular Endothelial Growth Factor A; D017228:Hepatocyte Growth Factor; D007334:Insulin-Like Growth Factor I; D005337:Fibrin
keywords: Growth factors; Inflammation; Leukocytes; Platelet rich plasma; Regenerative medicine