title: Molecular characterization and functional regulation of melanocortin 2 receptor (MC2R) in the sea bass. A putative role in the adaptation to stress.
abstract: The activation of melanocortin 2 receptor (MC2R) by ACTH mediates the signaling cascade leading to steroid synthesis in the interrenal tissue (analogous to the adrenal cortex in mammals) of fish. However, little is known about the functional regulation of this receptor in fish. In this work described, we cloned sea bass MC2R from a liver cDNA. SbMC2R requires the melanocortin 2 receptor accessory protein (MRAP) for its functional expression. Dietary cortisol but not long-term stress protocols downregulated interrenal sbMC2R expression. Data suggest the existence of a negative feedback on interrenal sbMC2R expression imposed by local or systemic glucocorticoids. This feedback could be involved in long-term stress adaptation by regulating interrenal sensitivity to ACTH. ACTH-induced MC2R activation stimulates hepatic lipolysis, suggesting that ACTH may mediate stress-induced effects upstream of cortisol release.
authors.affiliation: Department of Fish Physiology and Biotechnology, Instituto de Acuicultura de Torre de la Sal, Consejo Superior de Investigaciones Científicas-IATS-CSIC, Castellón, Spain.
authors.forename: Maria Josep
authors.initials: MJ
authors.lastname: Agulleiro
authors.forename: Elisa
authors.initials: E
authors.lastname: Sánchez
authors.forename: Esther
authors.initials: E
authors.lastname: Leal
authors.forename: Raúl
authors.initials: R
authors.lastname: Cortés
authors.forename: Begoña
authors.initials: B
authors.lastname: Fernández-Durán
authors.forename: Raúl
authors.initials: R
authors.lastname: Guillot
authors.forename: Perry
authors.initials: P
authors.lastname: Davis
authors.forename: Robert M
authors.initials: RM
authors.lastname: Dores
authors.forename: Nicole
authors.initials: N
authors.lastname: Gallo-Payet
authors.forename: José Miguel
authors.initials: JM
authors.lastname: Cerdá-Reverter
mesh_terms: D000220:Adaptation, Biological / Q000235:genetics*; D000324:Adrenocorticotropic Hormone / Q000494:pharmacology; D000595:Amino Acid Sequence; D000818:Animals; D001492:Bass / Q000235:genetics* / Q000378:metabolism*; D016466:CHO Cells; D003001:Cloning, Molecular; D003412:Cricetulus; D005215:Fasting; D015870:Gene Expression; D005786:Gene Expression Regulation / Q000187:drug effects; D050356:Lipid Metabolism / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D044103:Receptor, Melanocortin, Type 2 / Q000819:agonists / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D016415:Sequence Alignment; D013312:Stress, Physiological / Q000235:genetics*
chemical_list: D012333:RNA, Messenger; D044103:Receptor, Melanocortin, Type 2; D000324:Adrenocorticotropic Hormone