title: Effect of probiotics on the development of dimethylhydrazine-induced preneoplastic lesions in the mice colon.
abstract: OBJECTIVE
  To determine the effect of probiotics on the development of chemically induced (1, 2-dimethylhydrazine) colonic preneoplastic lesions, in mice.
  
  
  METHODS
  The animals were divided into five groups. The control group was injected with carcinogen alone and the other groups also received probiotics (1- Lactobacillus delbrueckii UFV-H2b20; 2- Bifidobacterium animalis var. lactis Bb12; 3- L. delbrueckii UFV-H2b20 plus B. animalis var. lactis Bb12; and 4- Saccharomyces boulardii) administered orally in drinking water throughout fourteen weeks.
  
  
  RESULTS
  Consumption of lactobacilli and bifidobacteria alone resulted in a significant reduction of the total number of aberrant crypt foci (55.7% and 45.1%, respectively). Significant reduction in the number of these small foci (<3 aberrant crypts) was only observed in the group treated with lactobacilli (52.2%) in comparison to control group. The number of larger foci (>3 aberrant crypts) crypts had no significant reduction.
  
  
  CONCLUSIONS
  L. delbrueckii UFV-H2b20 and B. animalis var. lactis Bb12 administered alone protect colonic preneoplastic lesions in mice, while the combined treatment of these bacteria and the administration of S.boulardii were not effective in reducing such colonic lesions.
authors.affiliation: Laboratory of Ecology and Physiology of Microorganisms, Department of Microbiology, Institute of Biological Sciences, Federal University of Minas Gerais/MG, Brazil.
authors.forename: Juliana Costa
authors.initials: JC
authors.lastname: Liboredo
authors.forename: Lucilene Rezende
authors.initials: LR
authors.lastname: Anastácio
authors.forename: Maria do Carmo Gouveia
authors.initials: Mdo C
authors.lastname: Pelúzio
authors.forename: Flávia Xavier
authors.initials: FX
authors.lastname: Valente
authors.forename: Luisa Costa Penna
authors.initials: LC
authors.lastname: Penido
authors.forename: Jacques Robert
authors.initials: JR
authors.lastname: Nicoli
authors.forename: Maria Isabel Toulson Davisson
authors.initials: MI
authors.lastname: Correia
mesh_terms: D058739:Aberrant Crypt Foci / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D000818:Animals; D001644:Bifidobacterium / Q000502:physiology; D002273:Carcinogens; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D003131:Combined Modality Therapy; D004127:Dimethylhydrazines; D048668:Lactobacillus delbrueckii / Q000502:physiology; D008297:Male; D051379:Mice; D011230:Precancerous Conditions / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D019936:Probiotics / Q000494:pharmacology*; D015203:Reproducibility of Results; D012440:Saccharomyces / Q000502:physiology; D013997:Time Factors
chemical_list: D002273:Carcinogens; D004127:Dimethylhydrazines