title: Functional analyse of GLUT1 and GLUT12 in glucose uptake in goat mammary gland epithelial cells.
abstract: Glucose transport, mediated by glucose transporters, is necessary for mammary gland development and lactation. GLUT1 and GLUT12 could both be expressed in the pregnant and lactating mammary gland to participate in the glucose uptake process. In this study, the goat GLUT1 and GLUT12 genes were cloned from Saanen dairy goats and transfected into goat mammary gland epithelial cells to assess their biological functions and distributions. The results showed that both goat GLUT1 and GLUT12 had 12 predicted membrane-spanning helices. Goat GLUT1 and GLUT12 each influenced the mRNA expression of the other transporter and increased the glucose consumption and lactose yield in GLUT1- and GLUT12-transfected goat mammary gland epithelial cells, respectively. The overexpression of GLUT1 or GLUT12 also increased the expression of amino acid transporters SLC1A5, SLC3A2 and SLC7A5 and affected genes expressions in GMGE cells. Using immunofluorescence staining, GLUT1 was detected throughout the cytoplasm and localized to the Golgi apparatus around the nuclear membrane, whereas GLUT12 was mainly distributed in the perinuclear region and cytoplasm. This study contributes to the understanding of how GLUT1 and GLUT12 cooperate in the incorporation of nutrient uptake into mammary gland epithelial cells and the promotion of milk synthesis in the goat mammary gland during lactation.
authors.affiliation: Veterinary College, Nanjing Agricultural University, Nanjing, Jiangsu, PR China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Yu
authors.forename: Liqi
authors.initials: L
authors.lastname: Zhu
authors.forename: Jian
authors.initials: J
authors.lastname: Lin
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.forename: Qi
authors.initials: Q
authors.lastname: Tian
authors.forename: Weiwei
authors.initials: W
authors.lastname: Hu
authors.forename: Qian
authors.initials: Q
authors.lastname: Yang
mesh_terms: D026905:Amino Acid Transport Systems / Q000235:genetics / Q000378:metabolism; D000818:Animals; D003001:Cloning, Molecular; D004847:Epithelial Cells / Q000378:metabolism*; D005260:Female; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D005947:Glucose / Q000378:metabolism*; D051246:Glucose Transport Proteins, Facilitative / Q000235:genetics / Q000378:metabolism*; D051272:Glucose Transporter Type 1 / Q000235:genetics / Q000378:metabolism*; D006041:Goats / Q000378:metabolism*; D007785:Lactose / Q000378:metabolism; D008321:Mammary Glands, Animal / Q000166:cytology*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis; D021381:Protein Transport; D012269:Ribosomal Proteins / Q000235:genetics / Q000378:metabolism; D020539:Sequence Analysis, Protein
chemical_list: D026905:Amino Acid Transport Systems; D051246:Glucose Transport Proteins, Facilitative; D051272:Glucose Transporter Type 1; D008565:Membrane Proteins; D012269:Ribosomal Proteins; D005947:Glucose; D007785:Lactose