title: A putative terpene cyclase, vir4, is responsible for the biosynthesis of volatile terpene compounds in the biocontrol fungus Trichoderma virens.
abstract: A putative terpene cyclase vir4, which is a member of a secondary metabolite cluster, has been deleted in Trichoderma virens to determine its function. The deletion mutants were compared for volatile production with the wild-type as well as two other Trichoderma spp. This gene cluster was originally predicted to function in the synthesis of viridin and viridiol. However, the experimental evidence demonstrates that this gene cluster is involved in the synthesis of volatile terpene compounds. The entire vir4-containing gene cluster is absent in two other species of Trichoderma, T. atroviride and T. reesei. Neither of these two species synthesizes volatile terpenes associated with this cluster in T. virens. We have thus identified a novel class of volatile fungal sesquiterpenes as well as the gene cluster involved in their biosynthesis.
authors.affiliation: Southern Plains Agricultural Research Center, USDA, Agricultural Research Service, 2765 F and B Road, College Station, TX 77845, United States.
authors.forename: Frankie K
authors.initials: FK
authors.lastname: Crutcher
authors.forename: Alexandra
authors.initials: A
authors.lastname: Parich
authors.forename: Rainer
authors.initials: R
authors.lastname: Schuhmacher
authors.forename: Prasun K
authors.initials: PK
authors.lastname: Mukherjee
authors.forename: Susanne
authors.initials: S
authors.lastname: Zeilinger
authors.forename: Charles M
authors.initials: CM
authors.lastname: Kenerley
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000235:genetics / Q000378:metabolism*; D017353:Gene Deletion; D053858:Metabolic Networks and Pathways / Q000235:genetics*; D005810:Multigene Family; D013729:Terpenes / Q000378:metabolism*; D014242:Trichoderma / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*
chemical_list: D013729:Terpenes; D019883:Alkyl and Aryl Transferases; C037934:terpene carbocyclase