title: Crystallographic analysis of new psychrophilic haloalkane dehalogenases: DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17.
abstract: Haloalkane dehalogenases are hydrolytic enzymes with a broad range of potential practical applications such as biodegradation, biosensing, biocatalysis and cellular imaging. Two newly isolated psychrophilic haloalkane dehalogenases exhibiting interesting catalytic properties, DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17, were purified and used for crystallization experiments. After the optimization of crystallization conditions, crystals of diffraction quality were obtained. Diffraction data sets were collected for native enzymes and complexes with selected ligands such as 1-bromohexane and 1,2-dichloroethane to resolutions ranging from 1.05 to 2.49 Å.
authors.affiliation: Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, CENAKVA and Institute of Complex Systems, Zamek 136, 373 33 Nove Hrady, Czech Republic.
authors.forename: Katsiaryna
authors.initials: K
authors.lastname: Tratsiak
authors.forename: Oksana
authors.initials: O
authors.lastname: Degtjarik
authors.forename: Ivana
authors.initials: I
authors.lastname: Drienovska
authors.forename: Lukas
authors.initials: L
authors.lastname: Chrast
authors.forename: Pavlina
authors.initials: P
authors.lastname: Rezacova
authors.forename: Michal
authors.initials: M
authors.lastname: Kuty
authors.forename: Radka
authors.initials: R
authors.lastname: Chaloupkova
authors.forename: Jiri
authors.initials: J
authors.lastname: Damborsky
authors.forename: Ivana
authors.initials: I
authors.lastname: Kuta Smatanova
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis / Q000737:chemistry*; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D006867:Hydrolases / Q000032:analysis / Q000737:chemistry*; D053520:Marinobacter / Q000201:enzymology*; D044096:Psychrobacter / Q000201:enzymology*; D014961:X-Ray Diffraction
chemical_list: D001426:Bacterial Proteins; D006867:Hydrolases; C046137:haloalkane dehalogenase
keywords: DmxA; DpcA; Marinobacter sp. ELB17; Psychrobacter cryohalolentis K5; haloalkane dehalogenases