title: The expression of laminin-5 in severe dysplasia/carcinoma in situ and early invasive squamous cell carcinoma: an immunohistochemical study.
abstract: OBJECTIVE
  The objective of the present study was to detect the immunohistochemical localization of laminin-5 in the subepithelial basement membrane and to investigate the integrity of the basement membrane in cases of severe dysplasia/carcinoma in situ and early invasive squamous cell carcinoma.
  
  
  METHODS
  Tissue samples of 55 filed (25-early invasive squamous cell carcinoma; 25-severe dysplasia/carcinoma in situ;5-normal buccal mucosa as controls) cases were collected from the archives of Oral Pathology Department. Routine staining and Immunostaining for laminin-5 (Biogenex) was carried out on the sections. The immunohistochemical localization and integrity of the basement membrane protein isoform laminin-5 was studied in formalin fixed paraffin embedded sections of severe dysplasia/CIS and early invasive squamous cell carcinoma (EISCC). Chi square test was used for statistical analysis. P values <0.05 were considered statistically significant.
  
  
  RESULTS
  The immunostaining of laminin-5 as observed under light microscope ranged from continuous to complete absence. The laminin-5 staining pattern was more often continuous in severe dysplasia/CIS and discontinuous or absent in EISCC. A statistical comparison between different scores of laminin-5 staining in severe dysplasia/CIS and early invasive squamous cell carcinoma showed a highly significant 'P' value (0.0001).
  
  
  CONCLUSIONS
  The status of basal lamina, laminin-5 may provide additional and relevant information about invasive potential of dysplasias. Hence the study of laminin-5 immunohistologically can be regarded as an adjunct to distinguish severe dysplastic lesions from early oral invasive carcinoma.
authors.affiliation: Department of Oral Pathology Jaipur Dental College and Hospital, Jaipur, India. drfarza@hotmail.com
authors.forename: F
authors.initials: F
authors.lastname: Rahman
authors.forename: N N
authors.initials: NN
authors.lastname: Rao
authors.forename: S R
authors.initials: SR
authors.lastname: Tippu
authors.forename: S
authors.initials: S
authors.lastname: Patil
authors.forename: S
authors.initials: S
authors.lastname: Agarwal
authors.forename: S
authors.initials: S
authors.lastname: Srivastava
mesh_terms: D001485:Basement Membrane / Q000737:chemistry / Q000473:pathology; D014408:Biomarkers, Tumor / Q000032:analysis; D002278:Carcinoma in Situ / Q000737:chemistry* / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000737:chemistry* / Q000473:pathology; D016022:Case-Control Studies; D015815:Cell Adhesion Molecules / Q000032:analysis*; D006801:Humans; D009061:Mouth Mucosa / Q000737:chemistry* / Q000473:pathology; D009062:Mouth Neoplasms / Q000737:chemistry* / Q000473:pathology; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000032:analysis*; D011230:Precancerous Conditions / Q000378:metabolism / Q000473:pathology; D000097794:Kalinin
chemical_list: D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules; D009363:Neoplasm Proteins; D000097794:Kalinin