title: Development of loop-mediated isothermal amplification for detection of Leifsonia xyli subsp. xyli in sugarcane.
abstract: Ratoon stunt, caused by the xylem-limited coryneform bacterium Leifsonia xyli subsp. xyli (Lxx), is a deep bacteriosis and prevalent in most of sugarcane-producing countries. Based on loop-mediated isothermal amplification (LAMP), we developed a method for detecting Lxx. The major advantages of the LAMP method are visual judgment by color and time saving with only 60 min for identification of Lxx and without the need for costly PCR apparatus and gel scanner. In the present study, positive and negative samples detected by the LAMP method were clearly distinguishable. When total DNA extracted from internode juice was used as the template, the sensitivity of LAMP was 10 times higher than that of the conventional PCR detection. The LAMP assay is a highly specific, rapid, and sensitive method for the diagnosis of ratoon stunt caused by Lxx in sugarcane. This is the first report of LAMP-based assay for the detection of Lxx in sugarcane.
authors.affiliation: Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Liping
authors.initials: L
authors.lastname: Xu
authors.forename: Jinlong
authors.initials: J
authors.lastname: Guo
authors.forename: Rukai
authors.initials: R
authors.lastname: Chen
authors.forename: Michael Paul
authors.initials: MP
authors.lastname: Grisham
authors.forename: Youxiong
authors.initials: Y
authors.lastname: Que
mesh_terms: D000192:Actinomycetales / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers / Q000235:genetics; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D010935:Plant Diseases / Q000382:microbiology; D031786:Saccharum / Q000235:genetics / Q000382:microbiology*
chemical_list: D017931:DNA Primers