title: Molecular genetic characterization of the biosynthesis cluster of a prenylated isoindolinone alkaloid aspernidine A in Aspergillus nidulans.
abstract: Aspernidine A is a prenylated isoindolinone alkaloid isolated from the model fungus Aspergillus nidulans. A genome-wide kinase knockout library of A. nidulans was examined, and it was found that a mitogen-activated protein kinase gene, mpkA, deletion strain produces aspernidine A. Targeted gene deletions were performed in the kinase deletion background to identify the gene cluster for aspernidine A biosynthesis. Intermediates were isolated from mutant strains which provided information about the aspernidine A biosynthesis pathway.
authors.affiliation: Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, Los Angeles, California 90089, USA.
authors.forename: Junko
authors.initials: J
authors.lastname: Yaegashi
authors.forename: Mike B
authors.initials: MB
authors.lastname: Praseuth
authors.forename: Shiaw-Wei
authors.initials: SW
authors.lastname: Tyan
authors.forename: James F
authors.initials: JF
authors.lastname: Sanchez
authors.forename: Ruth
authors.initials: R
authors.lastname: Entwistle
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Chiang
authors.forename: Berl R
authors.initials: BR
authors.lastname: Oakley
authors.forename: Clay C C
authors.initials: CC
authors.lastname: Wang
mesh_terms: D000470:Alkaloids / Q000737:chemistry* / Q000378:metabolism; D001233:Aspergillus nidulans / Q000737:chemistry* / Q000378:metabolism; D053898:Biosynthetic Pathways; D017353:Gene Deletion; D005800:Genes, Fungal; D026121:Indole Alkaloids / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D054833:Isoindoles / Q000737:chemistry* / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D054876:Prenylation
chemical_list: D000470:Alkaloids; D026121:Indole Alkaloids; D054833:Isoindoles; C551571:aspernidine A; D020928:Mitogen-Activated Protein Kinases