title: Depletion of regulatory T cells in a mouse experimental glioma model through anti-CD25 treatment results in the infiltration of non-immunosuppressive myeloid cells in the brain.
abstract: The recruitment and activation of regulatory T cells (Tregs) in the micro-environment of malignant brain tumors has detrimental effects on antitumoral immune responses. Hence, local elimination of Tregs within the tumor micro-environment represents a highly valuable tool from both a fundamental and clinical perspective. In the syngeneic experimental GL261 murine glioma model, Tregs were prophylactically eliminated through treatment with PC61, an anti-CD25 mAb. This resulted in specific elimination of CD4+CD25hiFoxp3+ Treg within brain-infiltrating lymphocytes and complete protection against subsequent orthotopic GL261 tumor challenge. Interestingly, PC61-treated mice also showed a pronounced infiltration of CD11b+ myeloid cells in the brain. Phenotypically, these cells could not be considered as Gr-1+ myeloid-derived suppressor cells (MDSC) but were identified as F4/80+ macrophages and granulocytes.
authors.affiliation: Laboratory for Thrombosis Research, Interdisciplinary Research Facility Life Sciences Kulak, E. Sabbelaan 53, 8500 Kortrijk, Belgium.
authors.forename: Wim
authors.initials: W
authors.lastname: Maes
authors.forename: Tina
authors.initials: T
authors.lastname: Verschuere
authors.forename: Anaïs
authors.initials: A
authors.lastname: Van Hoylandt
authors.forename: Louis
authors.initials: L
authors.lastname: Boon
authors.forename: Stefaan
authors.initials: S
authors.lastname: Van Gool
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D001932:Brain Neoplasms / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D039481:CD11b Antigen / Q000235:genetics / Q000276:immunology; D002465:Cell Movement / Q000187:drug effects / Q000276:immunology; D005910:Glioma / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D006801:Humans; D008212:Lymphocyte Depletion; D016246:Lymphocytes, Tumor-Infiltrating / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D051379:Mice; D022423:Myeloid Cells / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D009374:Neoplasms, Experimental; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects / Q000276:immunology / Q000473:pathology
chemical_list: D000911:Antibodies, Monoclonal; D039481:CD11b Antigen; C584092:PC61 monoclonal antibody