title: Behaviour of human mesenchymal stem cells on chemically synthesized HA-PCL scaffolds for hard tissue regeneration.
abstract: Our goal was to characterize the response of human mesenchymal stem cells (hMSCs) to a novel composite scaffold for bone tissue engineering. The hydroxyapatite-polycaprolactone (HA-PCL) composite scaffolds were prepared by a sol-gel method at room temperature and the scaffold morphology was investigated by scanning electron microscopy (SEM)/energy-dispersive spectroscopy (EDS) to validate the synthesis process. The response of two different lines of hMSCs, bone-marrow-derived human mesenchymal stem cells (BMSCs) and dental pulp stem cells (DPSCs) in terms of cell proliferation and differentiation into the osteoblastic phenotype, was evaluated using Alamar blue assay, SEM, histology and alkaline phosphatase activity. Our results indicate that tissue engineering by means of composite HA-PCL scaffolds may represent a new therapeutic strategy to repair craniofacial bone defects.
authors.affiliation: Institute of Composite and Biomedical Materials (IMCB), National Research Council of Italy (CNR), Naples, Italy.|Department of Neurosciences, Reproductive Sciences and Oral Sciences, University of Naples 'Federico II', Italy.|Department of Pediatric Surgery and Transplantation, Bambino Gesù Children's Hospital, Rome, Italy.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: D'Antò
authors.affiliation: Institute of Composite and Biomedical Materials (IMCB), National Research Council of Italy (CNR), Naples, Italy.
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Raucci
authors.affiliation: Institute of Composite and Biomedical Materials (IMCB), National Research Council of Italy (CNR), Naples, Italy.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Guarino
authors.affiliation: Department of Neurosciences, Reproductive Sciences and Oral Sciences, University of Naples 'Federico II', Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Martina
authors.affiliation: Department of Neurosciences, Reproductive Sciences and Oral Sciences, University of Naples 'Federico II', Italy.
authors.forename: Rosa
authors.initials: R
authors.lastname: Valletta
authors.affiliation: Institute of Composite and Biomedical Materials (IMCB), National Research Council of Italy (CNR), Naples, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Ambrosio
mesh_terms: D016475:3T3 Cells; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D016923:Cell Death / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology; D017886:Durapatite / Q000494:pharmacology*; D006801:Humans; D007150:Immunohistochemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D051379:Mice; D015676:Osteonectin / Q000378:metabolism; D011091:Polyesters / Q000494:pharmacology*; D013052:Spectrometry, X-Ray Emission; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D015676:Osteonectin; D011091:Polyesters; C016240:polycaprolactone; D017886:Durapatite; D000469:Alkaline Phosphatase
keywords: bone defects; composite material; dental pulp stem cells; human mesenchymal stem cells; hydroxyapatite; polymer; scaffold