title: Telomere length and telomerase activity; a Yin and Yang of cell senescence.
abstract: Telomeres are repeating DNA sequences at the tip ends of the chromosomes that are diverse in length and in humans can reach a length of 15,000 base pairs. The telomere serves as a bioprotective mechanism of chromosome attrition at each cell division. At a certain length, telomeres become too short to allow replication, a process that may lead to chromosome instability or cell death. Telomere length is regulated by two opposing mechanisms: attrition and elongation. Attrition occurs as each cell divides. In contrast, elongation is partially modulated by the enzyme telomerase, which adds repeating sequences to the ends of the chromosomes. In this way, telomerase could possibly reverse an aging mechanism and rejuvenates cell viability. These are crucial elements in maintaining cell life and are used to assess cellular aging. In this manuscript we will describe an accurate, short, sophisticated and cheap method to assess telomere length in multiple tissues and species. This method takes advantage of two key elements, the tandem repeat of the telomere sequence and the sensitivity of the qRT-PCR to detect differential copy numbers of tested samples. In addition, we will describe a simple assay to assess telomerase activity as a complementary backbone test for telomere length.
authors.affiliation: Department of Medicine, Albert Einstein College of Medicine, USA.
authors.forename: Mary Derasmo
authors.initials: MD
authors.lastname: Axelrad
authors.forename: Temuri
authors.initials: T
authors.lastname: Budagov
authors.forename: Gil
authors.initials: G
authors.lastname: Atzmon
mesh_terms: D016922:Cellular Senescence / Q000235:genetics / Q000502:physiology*; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007962:Leukocytes / Q000166:cytology / Q000201:enzymology / Q000648:ultrastructure; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D019098:Telomerase / Q000378:metabolism*; D016615:Telomere / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D020079:Terminal Repeat Sequences
chemical_list: D004247:DNA; D019098:Telomerase