title: Forming lipid bilayer membrane arrays on micropatterned polyelectrolyte film surfaces.
abstract: A novel method of forming lipid bilayer membrane arrays on micropatterned polyelectrolyte film surfaces is introduced. Polyelectrolyte films were fabricated by the layer-by-layer technique on a silicon oxide surface modified with a 3-aminopropyltriethoxysilane (APTES) monolayer. The surface pK(a) value of the APTES monolayer was determined by cyclic voltammetry to be approximately 5.61, on the basis of which a pH value of 2.0 was chosen for layer-by-layer assembly. Micropatterned polyelectrolyte films were obtained by deep-UV (254 nm) photolysis though a mask. Absorbed fluorescent latex beads were used to visualize the patterned surfaces. Lipid bilayer arrays were fabricated on the micropatterned surfaces by immersing the patterned substrates into a solution containing egg phosphatidylcholine vesicles. Fluorescence recovery after photobleaching studies yielded a lateral diffusion coefficient for probe molecules of 1.31±0.17 μm(2) s(-1) in the bilayer region, and migration of the lipid NBD PE in bilayer lipid membrane arrays was observed in an electric field.
authors.affiliation: School of Chemical Engineering and Technology, Harbin Institute of Technology, No. 92, West Da-Zhi Street, Harbin, 150001, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Xuejing
authors.initials: X
authors.lastname: Wang
authors.forename: Guodong
authors.initials: G
authors.lastname: Qi
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Han
mesh_terms: D004058:Diffusion; D004573:Electrolytes; D008051:Lipid Bilayers / Q000737:chemistry*; D010713:Phosphatidylcholines / Q000737:chemistry*; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D010782:Photolysis; D011437:Propylamines; D012821:Silanes / Q000737:chemistry; D012822:Silicon Dioxide; D013499:Surface Properties; D014466:Ultraviolet Rays
chemical_list: D004573:Electrolytes; D008051:Lipid Bilayers; C584395:N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D011437:Propylamines; D012821:Silanes; D012822:Silicon Dioxide; C477625:amino-propyl-triethoxysilane