title: [Effect of univalent cations on synthesis of surfactants by Acinetobacter calcoaceticus IMV B-7241].
abstract: The effect of univalent cations on activity of key enzymes of C2-metabolism has been investigated in the producer of biosurfactants, Acinetibacter calcoaceticus IMV B-7241 grown on ethanol. It was established that potassium cations are inhibitors of pyroquinolinequinone-dependent alcohol- and acetaldehyde dehydrogenases, the enzymes of biosynthesis of surface-active aminolipids (NADP-dependent glutamate dehydrogenase) and glycolipids (phosphoenopyruvate (PhEP)-carboxikinase), while ammonium cations are activators of these enzymes and PhEP-carboxylase. A decrease of potassium cations concentration in the cultivation medium to 1 mM and increase of the content of amine nitrogen to 10 mM as a result of potassium nitrate substitution by equimolar, as to nitrogen, urea concentration were accompanied by the increase of activity of enzymes of ethanol metabolism and SAS biosynthesis, as well as by the 2-fold increase of conditional concentration of the biosurfactants.
authors.forename: T P
authors.initials: TP
authors.lastname: Pirog
authors.forename: T A
authors.initials: TA
authors.lastname: Shevchuk
authors.forename: S I
authors.initials: SI
authors.lastname: Antoniuk
authors.forename: E Iu
authors.initials: EIu
authors.lastname: Kravchenko
authors.forename: G A
authors.initials: GA
authors.lastname: Iutinskaia
mesh_terms: D016954:Acinetobacter calcoaceticus / Q000187:drug effects* / Q000201:enzymology* / Q000254:growth & development; D000429:Alcohol Oxidoreductases / Q000037:antagonists & inhibitors / Q000378:metabolism; D000641:Ammonia / Q000378:metabolism / Q000494:pharmacology*; D002414:Cations, Monovalent; D003470:Culture Media; D004789:Enzyme Activation / Q000187:drug effects; D000431:Ethanol / Q000378:metabolism; D018086:Glutamate Dehydrogenase (NADP+) / Q000037:antagonists & inhibitors / Q000378:metabolism; D019764:Phosphoenolpyruvate Carboxykinase (ATP) / Q000037:antagonists & inhibitors / Q000378:metabolism; D010730:Phosphoenolpyruvate Carboxylase / Q000037:antagonists & inhibitors / Q000378:metabolism; D011188:Potassium / Q000378:metabolism / Q000494:pharmacology*; D013379:Substrate Specificity; D013501:Surface-Active Agents / Q000378:metabolism*; D014508:Urea / Q000378:metabolism
chemical_list: D002414:Cations, Monovalent; D003470:Culture Media; D013501:Surface-Active Agents; D000431:Ethanol; D000641:Ammonia; D014508:Urea; D000429:Alcohol Oxidoreductases; C048312:polyvinyl-alcohol dehydrogenase (acceptor); D018086:Glutamate Dehydrogenase (NADP+); D010730:Phosphoenolpyruvate Carboxylase; D019764:Phosphoenolpyruvate Carboxykinase (ATP); D011188:Potassium