title: [Peptides analysis in digested edible bird's nest by HPLC-MS].
abstract: Edible bird's nest contains lots of glycoproteins. The glycosylation inhomogeneity for glycoprotein often results in wide range of molecular weight and the difficulty for protein separation and charaterization. In this paper, proteins in the edible bird's nest were extracted using multiple extractions, and then digested by PNgase F and trypsin. The digest mixture was separated with HPLC, and peptides were identified based on MS/MS data searching. The results indicated that the extracted proteins were amount to 79.7% of total protein in the edible bird's nest. More than 20 species of peptides in the digested mixture were identified. The sequences of these peptides showed similarity with some proteins from Swiss-prot. The research indicated that deglycosylation, tryptic digestion coupled with HPLC-MS/MS is a proper strategy for characterization of proteins in the edible bird's nest.
authors.affiliation: College of Food Science and Engineering, Shandong Agricultural University, Taian 271018, China. rolinsnow@126.com
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.forename: Xiu-Le
authors.initials: XL
authors.lastname: Li
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Gao
authors.forename: Ying-Jun
authors.initials: YJ
authors.lastname: Kong
authors.forename: Ming-Lin
authors.initials: ML
authors.lastname: Wang
authors.forename: Gui-Feng
authors.initials: GF
authors.lastname: Zhang
authors.forename: Zhi-Guo
authors.initials: ZG
authors.lastname: Su
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001717:Birds; D002851:Chromatography, High Pressure Liquid; D006023:Glycoproteins / Q000737:chemistry; D013058:Mass Spectrometry; D008516:Medicine, Chinese Traditional; D010446:Peptide Fragments / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D059748:Proteolysis
chemical_list: D006023:Glycoproteins; D010446:Peptide Fragments