title: An in vivo transient expression system can be applied for rapid and effective selection of artificial microRNA constructs for plant stable genetic transformation.
abstract: The utility of artificial microRNAs (amiRNAs) to induce loss of gene function has been reported for many plant species, but expression efficiency of the different amiRNA constructs in different transgenic plants was less predictable. In this study, expressions of amiRNAs through the gene backbone of Arabidopsis miR168a were examined by both Agrobacterium-mediated transient expression and stable plant genetic transformation. A corresponding trend in expression of amiRNAs by the same amiRNA constructs between the transient and the stable expression systems was observed in the experiments. Plant genetic transformation of the constructs that were highly expressible in amiRNAs in the transient agro-infiltration assays resulted in generation of transgenic lines with high level of amiRNAs. This provides a simple method for rapid and effective selection of amiRNA constructs used for a time-consuming genetic transformation in plants.
authors.affiliation: Agriculture and Agri-Food Canada, Pacific Agri-Food Research Center, Summerland, British Columbia V0H 1Z0, Canada.
authors.forename: Basdeo
authors.initials: B
authors.lastname: Bhagwat
authors.forename: Ming
authors.initials: M
authors.lastname: Chi
authors.forename: Li
authors.initials: L
authors.lastname: Su
authors.forename: Haifeng
authors.initials: H
authors.lastname: Tang
authors.forename: Guiliang
authors.initials: G
authors.lastname: Tang
authors.forename: Yu
authors.initials: Y
authors.lastname: Xiang
mesh_terms: D015870:Gene Expression; D023061:Gene Order; D005822:Genetic Vectors; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D010944:Plants / Q000235:genetics* / Q000378:metabolism*; D030821:Plants, Genetically Modified; D018749:RNA, Plant / Q000235:genetics*; D011198:Solanum tuberosum / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism; D014170:Transformation, Genetic
chemical_list: D035683:MicroRNAs; D018749:RNA, Plant