title: Protective effects of ginaton on vascular endothelial cells injured by angiotensin II and hypoxia in vitro.
abstract: The objective of this study was to explore the protective effect and possible mechanism of Ginkgo biloba extract (Ginaton) on human vascular endothelial cells (VECs) injured by angiotensin II (Ang-II) and hypoxia. The human aortic VECs were divided into different groups to observe the changes in endothelin (ET), intracellular calcium concentration ([Ca(2+)]i), and mitochondrial membrane potential (MMP). The results showed that Ginaton had inhibited ET secretion induced by hypoxia and Ang-II (P < .01); the protective effects of mid (10 mg/mL) and low concentrations (5 mg/mL) of Ginaton was obviously higher than that of the high concentration (25 mg/mL); [Ca(2+)]i increased and MMP decreased significantly in both the hypoxia and the Ang-II groups (P < .01); however, the changes in [Ca(2+)]i and MMP could be meliorated by Ginaton. This study suggested that Ginaton could effectively protect VECs against injury, and lower dose would be used clinically rather than the higher dose for obtaining better results.
authors.affiliation: Aerospace and Diving Medical Center of Navy General Hospital, Beijing, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Han
authors.forename: Minggao
authors.initials: M
authors.lastname: Li
mesh_terms: D000804:Angiotensin II / Q000633:toxicity*; D015687:Cell Hypoxia; D002478:Cells, Cultured; D019610:Cytoprotection; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D019332:Endothelin-1 / Q000378:metabolism; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D013997:Time Factors
chemical_list: D004365:Drugs, Chinese Herbal; D019332:Endothelin-1; C526713:ginaton; D000804:Angiotensin II
keywords: Ang-II; Ginaton; calcium concentration; hypoxia; mitochondria membrane potential; vascular endothelial cells