title: Total and antigen- (fungi, mites and staphylococcal enterotoxins) specific IgEs in nasal polyps is related to local eosinophilic inflammation.
abstract: BACKGROUND
  Total and specific immunoglobulin E (IgE) in nasal polyps (NP) is related to local eosinophilic inflammation. However, it remains unclear which antigens cause a stronger accumulation of eosinophils in NP. We investigated whether local IgEs to specific antigens correlated with local eosinophil accumulation.
  
  
  METHODS
  Total IgE, antigen-specific IgEs [5 species of fungi, staphylococcal enterotoxins A and B (SEA and SEB), cedar pollen, ragweed pollen and house-dust mites] and eosinophil cationic protein (ECP) were measured in sinus mucosa homogenates and serum from 29 patients with chronic rhinosinusitis with NP (CRSwNP), 8 patients with allergic fungal rhinosinusitis (AFRS) and 8 control subjects without CRS. The level of ECP in NP was analyzed for correlations with the total and specific IgE levels in the serum and NP.
  
  
  RESULTS
  In CRSwNP, antigen-specific IgEs [Alternaria > mite (Dp) > Aspergillus > SEB > Penicillium > mite (Df), but not the other fungi, SEA or pollens] in the sinus mucosa correlated significantly with the local ECP. In AFRS, antigen-specific IgEs [Penicillium > mite (Df) > Alternaria, but not the other fungi, SEs or pollens] in the sinus mucosa correlated significantly with the local ECP.
  
  
  CONCLUSIONS
  Local total IgE and antigen (fungi, mites and SEs)-specific IgEs in NP are related to the local eosinophilic inflammation of AFRS and CRSwNP.
authors.affiliation: Department of Otorhinolaryngology, Jikei University School of Medicine, Tokyo, Japan. matuwaki@jikei.ac.jp
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Matsuwaki
authors.forename: Keisuke
authors.initials: K
authors.lastname: Uno
authors.forename: Tetsushi
authors.initials: T
authors.lastname: Okushi
authors.forename: Nobuyoshi
authors.initials: N
authors.lastname: Otori
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Moriyama
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D000941:Antigens / Q000276:immunology*; D004768:Enterotoxins / Q000276:immunology; D004804:Eosinophils / Q000276:immunology*; D005260:Female; D005658:Fungi / Q000276:immunology; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology*; D007249:Inflammation / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D008925:Mites / Q000276:immunology; D009298:Nasal Polyps / Q000276:immunology*; D012220:Rhinitis / Q000276:immunology; D012852:Sinusitis / Q000276:immunology
chemical_list: D000941:Antigens; D004768:Enterotoxins; D007073:Immunoglobulin E; C031818:enterotoxin B, staphylococcal