title: Triplex PCR assay for the rapid identification of 3 major Vibrio species, Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio fluvialis.
abstract: A triplex PCR assay was developed for the identification of 3 major Vibrio spp., Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio fluvialis by targeting their haemolysin, haem-utilizing, and central regulatory genes, respectively. This simple, rapid, sensitive, and specific assay using cell lysates from 227 samples established its usefulness in research and epidemiology.
authors.affiliation: Department of Human Health and Diseases, School of Biological Sciences and Biotechnology, Indian Institute of Advanced Research, Gandhinagar, Gujarat 382 007, India.
authors.forename: Kittappa
authors.initials: K
authors.lastname: Vinothkumar
authors.forename: Ashima Kushwaha
authors.initials: AK
authors.lastname: Bhardwaj
authors.forename: Thandavarayan
authors.initials: T
authors.lastname: Ramamurthy
authors.forename: Swapan Kumar
authors.initials: SK
authors.lastname: Niyogi
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D006460:Hemolysin Proteins / Q000235:genetics; D026901:Membrane Transport Proteins / Q000235:genetics; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods* / Q000592:standards; D020033:Protein Isoforms / Q000235:genetics; D012680:Sensitivity and Specificity; D014157:Transcription Factors / Q000235:genetics; D014733:Vibrio / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D014734:Vibrio cholerae / Q000235:genetics* / Q000302:isolation & purification; D014736:Vibrio parahaemolyticus / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D017931:DNA Primers; D004268:DNA-Binding Proteins; D006460:Hemolysin Proteins; C087477:HutA protein, Vibrio cholerae; D026901:Membrane Transport Proteins; D020033:Protein Isoforms; D014157:Transcription Factors; C084287:hlyA protein, Vibrio cholerae; C066719:toxR protein, bacteria
keywords: Diagnostics; Epidemiology; Haem-utilising gene; Haemolysin gene; Toxin-regulatory gene