title: Auto-aligning stimulated emission depletion microscope using adaptive optics.
abstract: Stimulated emission depletion (STED) microscopy provides diffraction-unlimited resolution in fluorescence microscopy. Imaging at the nanoscale, however, requires precise alignment of the depletion and excitation laser foci of the STED microscope. We demonstrate here that adaptive optics can be implemented to automatically align STED and confocal images with a precision of 4.3 ± 2.3 nm.
authors.affiliation: Department of Cell Biology, Yale School of Medicine, 333 Cedar Street, New Haven, Connecticut 06510, USA. travis.gould@yale.edu
authors.forename: Travis J
authors.initials: TJ
authors.lastname: Gould
authors.forename: Emil B
authors.initials: EB
authors.lastname: Kromann
authors.forename: Daniel
authors.initials: D
authors.lastname: Burke
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Booth
authors.forename: Joerg
authors.initials: J
authors.lastname: Bewersdorf
mesh_terms: D007091:Image Processing, Computer-Assisted; D007834:Lasers; D008856:Microscopy, Fluorescence / Q000379:methods*; D055620:Optical Phenomena