title: Multiphoton excitation of lanthanides.
abstract: Without requiring chelation by sensitizers, a method to detect lanthanides by multiphoton excitation is reported. This suggests the use of lanthanides as calcium analogues in multiphoton microscopy. The spectrum shows the normalized two-photon excitation emission spectra (λex = 796 nm) of Eu(3+) with a resolution of about 8 nm.
authors.affiliation: Center for Fluorescence Spectroscopy, Deptartment of Biochemistry and Molecular Biology, School of Medicine, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, MD 21201, USA. cfs@cfs.umbi.umd.edu
authors.forename: J R
authors.initials: JR
authors.lastname: Lakowicz
authors.forename: G
authors.initials: G
authors.lastname: Piszczek
authors.forename: B P
authors.initials: BP
authors.lastname: Maliwal
authors.forename: I
authors.initials: I
authors.lastname: Gryczynski
mesh_terms: D002118:Calcium / Q000737:chemistry; D005063:Europium / Q000737:chemistry; D028581:Lanthanoid Series Elements / Q000737:chemistry*; D036641:Microscopy, Fluorescence, Multiphoton; D013725:Terbium / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D028581:Lanthanoid Series Elements; D014867:Water; D013725:Terbium; D005063:Europium; D002118:Calcium