title: Purification, crystallization and preliminary X-ray diffraction analysis of Omp6, a protoilludene synthase from Omphalotus olearius.
abstract: Basidiomycetes produce a wide range of industrially relevant natural products. One of the main classes of natural products isolated from fungi are terpenoids, a highly diverse group of secondary metabolites, many of which are bioactive and have been adapted for pharmaceutical purposes. The discovery of a suite of novel sesquiterpene synthases from Omphalotus olearius via genome sequencing and bioinformatic analyses has recently been described. Here, the expression, purification and crystallization of one of these enzymes (Omp6), a protoilludene synthase, is reported. A native crystal diffracted to a resolution of 2.9 Å and belonged to space group P21, with unit-cell parameters a = 43.67, b = 76.76, c = 107.22 Å, α = γ = 90, β = 95°. A diffraction data set was collected on a home-source Rigaku/MSC MicroMax-007 X-ray generator.
authors.affiliation: BMBB/BTI, University of Minnesota, 1479 Gortner Avenue, Saint Paul, MN 55108, USA. mbquin@umn.edu
authors.forename: Maureen B
authors.initials: MB
authors.lastname: Quin
authors.forename: Grayson
authors.initials: G
authors.lastname: Wawrzyn
authors.forename: Claudia
authors.initials: C
authors.lastname: Schmidt-Dannert
mesh_terms: D001487:Basidiomycota / Q000201:enzymology*; D003460:Crystallization; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification; D014961:X-Ray Diffraction
chemical_list: D005656:Fungal Proteins; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes; C506221:protoilludane
keywords: Omphalotus olearius; natural products; protoilludene; sesquiterpene synthases