title: Caffeic acid phenethyl ester inhibits endothelial tissue factor expression.
abstract: Caffeic acid phenethyl ester (CAPE) is a component of honeybee hives with various beneficial properties. Tissue factor (TF), the key trigger of thrombosis, is expressed in human endothelial cells. This study was designed to investigate whether CAPE modulates TF expression in human aortic endothelial cells (HAECs). Western blots and real-time polymerase chain reactions were performed. CAPE (10(-7)-10(-5) M) inhibited tumor necrosis factor (TNF)-α induced endothelial TF protein expression by 2.1-fold at 10(-5) M (p<0.0001). Similarly, TF surface activity was reduced (p<0.02). In contrast, TF mRNA expression, TF promoter activity, and mitogen-activated protein (MAP) kinase activation remained unaltered. In conclusion, CAPE inhibits TF protein expression and activity at the posttranscriptional level thereby exhibiting anti-thrombotic potential.
authors.affiliation: Cardiovascular Research, Physiology Institute, University of Zürich, Zürich, Switzerland.
authors.forename: Cathérine
authors.initials: C
authors.lastname: Gebhard
authors.forename: Barbara Elisabeth
authors.initials: BE
authors.lastname: Stähli
authors.forename: Stephanie
authors.initials: S
authors.lastname: Largiadèr
authors.forename: Erik Walter
authors.initials: EW
authors.lastname: Holy
authors.forename: Alexander
authors.initials: A
authors.lastname: Akhmedov
authors.forename: Giovanni Guido
authors.initials: GG
authors.lastname: Camici
authors.forename: Thomas Felix
authors.initials: TF
authors.lastname: Lüscher
authors.forename: Felix Christoph
authors.initials: FC
authors.lastname: Tanner
mesh_terms: D001011:Aorta / Q000166:cytology; D002109:Caffeic Acids / Q000494:pharmacology*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D005343:Fibrinolytic Agents / Q000494:pharmacology*; D006801:Humans; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D013925:Thromboplastin / Q000037:antagonists & inhibitors* / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1 / Q000235:genetics
chemical_list: D002109:Caffeic Acids; D005343:Fibrinolytic Agents; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1; D013925:Thromboplastin; D020928:Mitogen-Activated Protein Kinases; C055494:caffeic acid phenethyl ester; D010626:Phenylethyl Alcohol