title: PCR amplification and sequence analysis of GC-rich sequences: Aristaless-related homeobox example.
abstract: PCR amplification (followed by mutation scanning or direct sequencing) is a technique widely used in mutation detection and molecular studies of disease-causing genes, such as ARX. PCR amplification of high GC-rich regions encounters difficulties using conventional PCR procedures. Here, we present the strategies to amplify and sequence these GC-rich regions for the purposes of mutation screening and other molecular analyses.
authors.affiliation: Department of Paediatrics, University of Adelaide, Adelaide, SA, Australia.
authors.forename: May H
authors.initials: MH
authors.lastname: Tan
authors.forename: Jozef
authors.initials: J
authors.lastname: Gécz
authors.forename: Cheryl
authors.initials: C
authors.lastname: Shoubridge
mesh_terms: D004252:DNA Mutational Analysis / Q000379:methods; D020862:GC Rich Sequence; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D014157:Transcription Factors / Q000235:genetics*
chemical_list: C469264:ARX protein, human; D018398:Homeodomain Proteins; D014157:Transcription Factors