title: miR-9 functions as a tumor suppressor in ovarian serous carcinoma by targeting TLN1.
abstract: microRNAs (miRNAs) are important regulators of gene expression during tumorigenesis. The downregulation of microRNA-9 (miR-9) has been reported in ovarian serous carcinoma (OSC), indicating a role for miR-9 in this type of cancer. In this study, we investigated the biological significance of miR-9 in OSC in vitro. Using 3 OSC cell lines, SKOV3, CAOV3 and OVCAR3, which underexpresss miR-9, we demonstrate that the exogenous miR-9 transfection inhibits OSC cell proliferation, migration and invasion. In addition, we demonstrate that the focal adhesion protein, talin 1 (TLN1), whose expression has been associated with OSC development and progression to metastasis, is a direct target of miR-9. TLN1 knockdown mimicked the effects of miR-9 overexpression. Moreover, the activation of the TLN1-modulated FAK/AKT pathway was inhibited by the increased miR-9 levels. These results suggest that miR-9 plays a role as a tumor suppressor in OSC by suppressing TLN1 expression.
authors.affiliation: Department of Obstetrics and Gynecology, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, P.R. China.
authors.forename: Haosha
authors.initials: H
authors.lastname: Tang
authors.forename: Liangqing
authors.initials: L
authors.lastname: Yao
authors.forename: Xiang
authors.initials: X
authors.lastname: Tao
authors.forename: Yi
authors.initials: Y
authors.lastname: Yu
authors.forename: Mo
authors.initials: M
authors.lastname: Chen
authors.forename: Rong
authors.initials: R
authors.lastname: Zhang
authors.forename: Congjian
authors.initials: C
authors.lastname: Xu
mesh_terms: D020413:3' Untranslated Regions; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D018293:Cystadenoma, Serous / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D018450:Disease Progression; D005260:Female; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism; D015870:Gene Expression; D015972:Gene Expression Regulation, Neoplastic; D016147:Genes, Tumor Suppressor; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D060787:Neoplasm Grading; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D034622:RNA Interference; D015398:Signal Transduction; D016608:Talin / Q000235:genetics* / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; C541874:MIRN92 microRNA, human; D035683:MicroRNAs; C518832:TLN1 protein, human; D016608:Talin; D051416:Focal Adhesion Protein-Tyrosine Kinases; D051057:Proto-Oncogene Proteins c-akt