title: Ca²⁺ is involved in phytochrome A-dependent regulation of the succinate dehydrogenase gene sdh1-2 in Arabidopsis.
abstract: The mechanism of transduction of the phytochrome signal regulating the expression of succinate dehydrogenase in Arabidopsis has been investigated. Using the phytochrome mutants of Arabidopsis, it is demonstrated that the inhibition of succinate dehydrogenase in the light may result from the phytochrome A-dependent modulation of Ca²⁺ amount in the nuclear fraction of leaves. This leads to the activation of expression of the gene pif3 encoding the phytochrome-interacting factor PIF3, which binds to the promoter of the gene sdh1-2 encoding the SDHA subunit of succinate dehydrogenase and suppresses its expression. It is concluded that Ca²⁺ ions are involved in the phytochrome A-mediated inhibition of succinate dehydrogenase activity in the light.
authors.affiliation: Department of Biochemistry and Cell Physiology, Voronezh State University, 394006 Voronezh, Russia.
authors.forename: Alexander T
authors.initials: AT
authors.lastname: Eprintsev
authors.forename: Dmitry N
authors.initials: DN
authors.lastname: Fedorin
authors.forename: Abir U
authors.initials: AU
authors.lastname: Igamberdiev
mesh_terms: D017360:Arabidopsis / Q000201:enzymology* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D051942:Phytochrome A / Q000378:metabolism*; D013385:Succinate Dehydrogenase / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D051942:Phytochrome A; D013385:Succinate Dehydrogenase; D002118:Calcium
keywords: Arabidopsis thaliana; Calcium; EGTA; Gene expression; PIF; Phytochrome; SDH; Succinate dehydrogenase; ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid; phytochrome-interacting factor; succinate dehydrogenase