title: Presence of acyl-homoserine lactones in 57 members of the Vibrionaceae family.
abstract: OBJECTIVE
  The aim of this study was to use a sensitive method to screen and quantify 57 Vibrionaceae strains for the production of acyl-homoserine lactones (AHLs) and map the resulting AHL profiles onto a host phylogeny.
  
  
  RESULTS
  We used a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) protocol to measure AHLs in spent media after bacterial growth. First, the presence/absence of AHLs (qualitative analysis) was measured to choose internal standard for subsequent quantitative AHL measurements. We screened 57 strains from three genera (Aliivibrio, Photobacterium and Vibrio) of the same family (i.e. Vibrionaceae). Our results show that about half of the isolates produced multiple AHLs, typically at 25-5000 nmol l(-1) .
  
  
  CONCLUSIONS
  This work shows that production of AHL quorum sensing signals is found widespread among Vibrionaceae bacteria and that closely related strains typically produce similar AHL profiles.
  
  
  CONCLUSIONS
  The AHL detection protocol presented in this study can be applied to a broad range of bacterial samples and may contribute to a wider mapping of AHL production in bacteria, for example, in clinically relevant strains.
authors.affiliation: The Norwegian Structural Biology Centre, University of Tromsø, Tromsø, Norway.
authors.forename: A A
authors.initials: AA
authors.lastname: Purohit
authors.forename: J A
authors.initials: JA
authors.lastname: Johansen
authors.forename: H
authors.initials: H
authors.lastname: Hansen
authors.forename: H-K S
authors.initials: HK
authors.lastname: Leiros
authors.forename: A
authors.initials: A
authors.lastname: Kashulin
authors.forename: C
authors.initials: C
authors.lastname: Karlsen
authors.forename: A
authors.initials: A
authors.lastname: Smalås
authors.forename: P
authors.initials: P
authors.lastname: Haugen
authors.forename: N P
authors.initials: NP
authors.lastname: Willassen
mesh_terms: D054742:Acyl-Butyrolactones / Q000032:analysis* / Q000378:metabolism; D048908:Aliivibrio fischeri / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D013058:Mass Spectrometry; D010776:Photobacterium / Q000737:chemistry; D053038:Quorum Sensing; D053719:Tandem Mass Spectrometry; D014733:Vibrio / Q000737:chemistry; D014737:Vibrionaceae / Q000737:chemistry / Q000145:classification / Q000378:metabolism*
chemical_list: D054742:Acyl-Butyrolactones
keywords: AHLs; HPLC-MS/MS; Vibrionaceae; acyl-homoserine lactones; quorum sensing