title: Immobilization of double-stranded DNA onto glass beads by psolaren.
abstract: We demonstrated the immobilization of double-stranded DNA onto the glass beads by psoralen, one of the DNA-intercalators in nature. As a result, DNA-immobilized glass beads (DNA-P-beads) were prepared by the intercalation of psoralen, which was immobilized onto the glass surface, onto the double-stranded DNA. These DNA-P-beads formed covalent bondings between psoralen and the nucleic acid base by 365 nm UV irradiation. The amount of immobilized-DNA was 0.24 mg per gram of glass beads. These DNA-P-beads were stable in water, and the DNA on the bead surface maintained its double-stranded structure. These DNA-P-beads selectively removed the planar-structure containing harmful compounds, such as dioxin and polychlorinated biphenyl (PCB) derivatives, from an aqueous multi-component solution. Additionally, a DNA-P-bead column effectively removed harmful compounds. Furthermore, the DNA-P-bead column could be reused by the addition of common organic solvents, such as ethanol.
authors.affiliation: Department of Chemistry, Faculty of Science, Okayama University of Science, Ridaicho, Kita-Ku, Okayama 700-0005, Japan. myamada@chem.ous.ac.jp
authors.forename: Masanori
authors.initials: M
authors.lastname: Yamada
authors.forename: Yuka
authors.initials: Y
authors.lastname: Kanamori
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Yamada
mesh_terms: D004247:DNA / Q000737:chemistry*; D005363:Ficusin / Q000138:chemical synthesis / Q000737:chemistry*; D005898:Glass / Q000737:chemistry*; D007364:Intercalating Agents / Q000737:chemistry*
chemical_list: D007364:Intercalating Agents; D004247:DNA; D005363:Ficusin
keywords: DNA; Environmental material; Immobilization; Intercalation; Psoralen