title: The role of sulfur dioxide in the regulation of mitochondrion-related cardiomyocyte apoptosis in rats with isopropylarterenol-induced myocardial injury.
abstract: The authors investigated the regulatory effects of sulfur dioxide (SO2) on myocardial injury induced by isopropylarterenol (ISO) hydrochloride and its mechanisms. Wistar rats were divided into four groups: control group, ISO group, ISO plus SO2 group, and SO2 only group. Cardiac function was measured and cardiomyocyte apoptosis was detected. Bcl-2, bax and cytochrome c (cytc) expressions, and caspase-9 and caspase-3 activities in the left ventricular tissues were examined in the rats. The opening status of myocardial mitochondrial permeability transition pore (MPTP) and membrane potential were analyzed. The results showed that ISO-treated rats developed heart dysfunction and cardiac injury. Furthermore, cardiomyocyte apoptosis in the left ventricular tissues was augmented, left ventricular tissue bcl-2 expression was down-regulated, bax expression was up-regulated, mitochondrial membrane potential was significantly reduced, MPTP opened, cytc release from mitochondrion into cytoplasm was significantly increased, and both caspase-9 and caspase-3 activities were increased. Administration of an SO2 donor, however, markedly improved heart function and relieved myocardial injury of the ISO-treated rats; it lessened cardiomyocyte apoptosis, up-regulated myocardial bcl-2, down-regulated bax expression, stimulated mitochondrial membrane potential, closed MPTP, and reduced cytc release as well as caspase-9 and caspase-3 activities in the left ventricular tissue. Hence, SO2 attenuated myocardial injury in association with the inhibition of apoptosis in myocardial tissues, and the bcl-2/cytc/caspase-9/caspase-3 pathway was possibly involved in this process.
authors.affiliation: Department of Pediatrics, Peking University First Hospital, Xi'an Men Str. No. 1, West District, Beijing 100034, China. junbaodu1@126.com.
authors.forename: Hongfang
authors.initials: H
authors.lastname: Jin
authors.forename: Angie Dong
authors.initials: AD
authors.lastname: Liu
authors.forename: Lukas
authors.initials: L
authors.lastname: Holmberg
authors.forename: Manman
authors.initials: M
authors.lastname: Zhao
authors.forename: Siyao
authors.initials: S
authors.lastname: Chen
authors.forename: Jinyan
authors.initials: J
authors.lastname: Yang
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.forename: Shanshan
authors.initials: S
authors.lastname: Chen
authors.forename: Chaoshu
authors.initials: C
authors.lastname: Tang
authors.forename: Junbao
authors.initials: J
authors.lastname: Du
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D009202:Cardiomyopathies / Q000139:chemically induced / Q000503:physiopathology / Q000517:prevention & control*; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D003402:Creatine Kinase / Q000097:blood; D045304:Cytochromes c / Q000378:metabolism; D004452:Echocardiography; D007274:Injections, Intraperitoneal; D007545:Isoproterenol; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008929:Mitochondria, Heart / Q000378:metabolism*; D033681:Mitochondrial Membrane Transport Proteins / Q000378:metabolism; D000083162:Mitochondrial Permeability Transition Pore; D032383:Myocytes, Cardiac / Q000166:cytology / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D017208:Rats, Wistar; D013447:Sulfites / Q000008:administration & dosage / Q000378:metabolism; D013458:Sulfur Dioxide / Q000378:metabolism / Q000494:pharmacology*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
chemical_list: D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D019253:Proto-Oncogene Proteins c-bcl-2; D013447:Sulfites; D051028:bcl-2-Associated X Protein; D013458:Sulfur Dioxide; D045304:Cytochromes c; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase; D053148:Caspase 3; D053453:Caspase 9; D007545:Isoproterenol; C009279:sodium bisulfite; C025026:sodium sulfite