title: Stearylated antimicrobial peptide [D]-K6L9 with cell penetrating property for efficient gene transfer.
abstract: Stearyl-cell penetrating peptides (CPPs) have been proved to be efficient nonviral gene vectors. Due to the similarities between antimicrobial peptides and CPPs, we constructed a novel type of gene vectors by introducing stearyl moiety to the N-terminus of antimicrobial peptide [D]-K6L9. In this study, stearyl-[D]-K6L9 delivered plasmids into cells by clathrin- and caveolin-mediated endocytosis. Gratifyingly, stearyl-[D]-K6L9 exhibited high transfection efficiency and almost reached the level of Lipofectamine 2000. Taken together, the combination of the stearyl moiety with [D]-K6L9 provides a novel framework for the development of excellent nonviral gene vectors.
authors.affiliation: The First Clinical Medical School, Lanzhou University, Lanzhou 730000, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Jingjing
authors.initials: J
authors.lastname: Song
authors.forename: Ranran
authors.initials: R
authors.lastname: Liang
authors.forename: Xin
authors.initials: X
authors.lastname: Zheng
authors.forename: Jianbo
authors.initials: J
authors.lastname: Chen
authors.forename: Guolin
authors.initials: G
authors.lastname: Li
authors.forename: Bangzhi
authors.initials: B
authors.lastname: Zhang
authors.forename: Kairong
authors.initials: K
authors.lastname: Wang
authors.forename: Xiang
authors.initials: X
authors.lastname: Yan
authors.forename: Rui
authors.initials: R
authors.lastname: Wang
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics* / Q000378:metabolism; D016466:CHO Cells; D019556:COS Cells; D002460:Cell Line; D057846:Cell-Penetrating Peptides / Q000235:genetics* / Q000378:metabolism; D002522:Chlorocebus aethiops; D003412:Cricetulus; D004705:Endocytosis / Q000187:drug effects / Q000502:physiology; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics; D008055:Lipids
chemical_list: D023181:Antimicrobial Cationic Peptides; D057846:Cell-Penetrating Peptides; D008055:Lipids; C086724:Lipofectamine; C485184:enhanced green fluorescent protein; C587489:stearyl-(D)-K6L9 peptide; D049452:Green Fluorescent Proteins
keywords: Antimicrobial peptide; Cell penetrating peptide; Endocytosis; Nonviral gene vector; Stearylation