title: A disposable electrochemiluminescence device for ultrasensitive monitoring of K562 leukemia cells based on aptamers and ZnO@carbon quantum dots.
abstract: We developed a new electrochemiluminescence (ECL) platform for ultrasensitive and selective detection of leukemia cells. In order to construct the platform, the nonporous gold with controllable three-dimensional porosity and good conductivity was used to modify the screen-printed carbon electrode. The carbon quantum dots (CQDs) coated ZnO nanosphere (ZnO@CQDs) were used as good ECL label with low cytotoxicity and good biocompatibility. Structure characterization was obtained by means of transmission electron microscopy and scanning electron microscopy images. The aptamer was used for cell capture and the concanavalin A conjugated ZnO@CQDs was used for selective recognition of the cell surface carbohydrate. The proposed method showed a good analytical performance for the detection of K562 cells ranging from 1.0 × 10(2) to 2.0 × 10(7) cells mL(-1) with a detection limit of 46 cells mL(-1). The as-proposed device has the advantages of high sensitivity, nice specificity and good stability and could offer great promise for sensitive detection of leukemia cells in response to therapy.
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Zhang
authors.forename: Heng
authors.initials: H
authors.lastname: Liu
authors.forename: Long
authors.initials: L
authors.lastname: Chen
authors.forename: Mei
authors.initials: M
authors.lastname: Yan
authors.forename: Lei
authors.initials: L
authors.lastname: Ge
authors.forename: Shenguang
authors.initials: S
authors.lastname: Ge
authors.forename: Jinghua
authors.initials: J
authors.lastname: Yu
mesh_terms: D052157:Aptamers, Nucleotide / Q000378:metabolism; D015374:Biosensing Techniques / Q000295:instrumentation*; D002244:Carbon / Q000737:chemistry*; D002469:Cell Separation / Q000295:instrumentation; D003208:Concanavalin A / Q000378:metabolism; D004867:Equipment Design; D006801:Humans; D020014:K562 Cells; D007938:Leukemia / Q000175:diagnosis / Q000473:pathology*; D008163:Luminescent Measurements / Q000295:instrumentation*; D045663:Quantum Dots; D012680:Sensitivity and Specificity; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D052157:Aptamers, Nucleotide; D003208:Concanavalin A; D002244:Carbon; D015034:Zinc Oxide
keywords: Aptamer; Carbon quantum dots; Electrochemiluminescence; K562 cells; ZnO nanosphere