title: Antiallergic effect of anti-Siglec-F through reduction of eosinophilic inflammation in murine allergic rhinitis.
abstract: BACKGROUND
  Sialic acid-binding Ig-like lectin-F (Siglec-F) in mice and its functional paralog Siglec-8 in humans are transmembrane receptors that play a role in the apoptosis of eosinophils. We aimed to evaluate the therapeutic potential of anti-Siglec-F antibodies in a murine model of allergic rhinitis.
  
  
  METHODS
  Twenty-eight BALB/c mice were used. In group A (control group, n = 7), mice were sensitized and challenged with saline. In group B (ovalbumin [OVA] challenge group, n = 7), OVA was used for i.p. sensitization and intranasal challenge. Mice in group C (control IgG group, n = 7) or those in group D (anti-Siglec-F group, n = 7) had been given rabbit control IgG or anti-Siglec-F antibody injections, respectively. We assessed the number of nose-scratching events; serum total/OVA-specific IgE; the number of eosinophils, neutrophils, and lymphocytes in bronchoalveolar lavage (BAL) fluid; histopathological changes in nasal cavity tissues; and the levels of IL-4, IL-5, and IL-13 in BAL fluid.
  
  
  RESULTS
  Mice in group D had significantly less nose scratching. Serum total and OVA-specific IgE were not significantly changed. The number of eosinophils in BAL fluid and in the lamina propria of the nasal cavity mucosa was significantly decreased with anti-Siglec-F antibody treatment. The levels of Th2 cytokines such as IL-4, IL-5, and IL-13 were also significantly decreased with anti-Siglec-F antibody treatment.
  
  
  CONCLUSIONS
  Anti-Siglec-F antibody has beneficial effects in a mouse model of experimental allergic rhinitis.
authors.affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Inha University College of Medicine, Incheon, Korea.
authors.forename: Young Hyo
authors.initials: YH
authors.lastname: Kim
authors.forename: Chang-Shin
authors.initials: CS
authors.lastname: Park
authors.forename: Dae Hyun
authors.initials: DH
authors.lastname: Lim
authors.forename: Byong Kwan
authors.initials: BK
authors.lastname: Son
authors.forename: Jeong Hee
authors.initials: JH
authors.lastname: Kim
authors.forename: Sung-Hye
authors.initials: SH
authors.lastname: Ahn
authors.forename: Bruce S
authors.initials: BS
authors.lastname: Bochner
authors.forename: Kwangmin
authors.initials: K
authors.lastname: Na
authors.forename: Tae Young
authors.initials: TY
authors.lastname: Jang
mesh_terms: D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D015214:Antigens, Differentiation, Myelomonocytic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D004195:Disease Models, Animal; D004804:Eosinophils / Q000187:drug effects*; D007249:Inflammation / Q000188:drug therapy; D051379:Mice; D008807:Mice, Inbred BALB C; D065631:Rhinitis, Allergic; D012221:Rhinitis, Allergic, Perennial / Q000188:drug therapy*; D063265:Sialic Acid Binding Immunoglobulin-like Lectins / Q000037:antagonists & inhibitors*; D016896:Treatment Outcome
chemical_list: D018926:Anti-Allergic Agents; D015214:Antigens, Differentiation, Myelomonocytic; D063265:Sialic Acid Binding Immunoglobulin-like Lectins; C443130:Siglecf protein, mouse