title: Efficacy of polymer coating of probiotic beads suspended in pressurized and pasteurized longan juices on the exposure to simulated gastrointestinal environment.
abstract: Alginate-coated Lactobacillus acidophilus LA5 or Lactobacillus casei 01 was recoated with either 0.1-0.5% (w/v) alginate or 0.05-0.15% (w/v) poly-L-lysine (PLL) plus 0.2% (w/v) alginate or 5-15% (w/v) gelatin, after which they were determined for survivability in gastric or bile longan juices. The morphology of encapsulated probiotic cells illustrated that recoated beads with 0.5% alginate showed a more compact surface and a greater protective effect than other recoating materials. The recoated beads with 0.5% alginate and 0.05-0.15% PLL plus 0.2% alginate of both strains showed the highest viability in gastric longan juice. In bile longan juice, only 0.5% alginate showed the best protection for both recoated beads. When considering the storage stability, encapsulated L. acidophilus LA5 exhibited a higher viable count than those of the free cells, whereas L. casei 01 showed equivalent viability of both free and double-coated cells. Based on the impact of pressurization or pasteurization, both processed juices gave rise to equivalent survivability of the probiotic cells during storage.
authors.affiliation: Division of Food Studies, Science and Technology Research Institute, Chiang Mai University , Chiang Mai , Thailand .
authors.forename: Pittaya
authors.initials: P
authors.lastname: Chaikham
authors.forename: Arunee
authors.initials: A
authors.lastname: Apichartsrangkoon
authors.forename: Trevor
authors.initials: T
authors.lastname: George
authors.forename: Wachira
authors.initials: W
authors.lastname: Jirarattanarangsri
mesh_terms: D000464:Alginates; D001646:Bile; D002214:Capsules; D005750:Gastric Juice; D041981:Gastrointestinal Tract; D007779:Lactobacillus acidophilus; D007780:Lacticaseibacillus casei; D008239:Lysine; D050296:Microbial Viability; D061352:Pasteurization; D011107:Polylysine / Q000031:analogs & derivatives; D011108:Polymers; D011312:Pressure; D019936:Probiotics / Q000008:administration & dosage*; D013499:Surface Properties
chemical_list: D000464:Alginates; D002214:Capsules; D011108:Polymers; C051546:alginate-polylysine-alginate; D011107:Polylysine; D008239:Lysine