title: Genetic analysis of the VP2-encoding gene of canine parvovirus strains from Africa.
abstract: Since the emergence of canine parvovirus type-2 (CPV-2) in the early 1970s, it has been evolving into novel genetic and antigenic variants (CPV-2a, 2b and 2c) that are unevenly distributed throughout the world. Genetic characterization of CPV-2 has not been documented in Africa since 1998 apart from the study carried out in Tunisia 2009. A total of 139 field samples were collected from South Africa and Nigeria, detected using PCR and the full length VP2-encoding gene of 27 positive samples were sequenced and genetically analyzed. Nigerian samples (n=6), South Africa (n=19) and vaccine strains (n=2) were compared with existing sequences obtained from GenBank. The results showed the presence of both CPV-2a and 2b in South Africa and only CPV-2a in Nigeria. No CPV-2c strain was detected during this study. Phylogenetic analysis showed a clustering not strictly associated with the geographical origin of the analyzed strains, although most of the South African strains tended to cluster together and the viral strains analyzed in this study were not completely distinct from CPV-2 strains from other parts of the world. Amino acid analysis showed predicted amino acid changes.
authors.affiliation: Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort 0110, South Africa. bbdogonyaro@gmail.com
authors.forename: Banenat B
authors.initials: BB
authors.lastname: Dogonyaro
authors.forename: Anna-Mari
authors.initials: AM
authors.lastname: Bosman
authors.forename: Kgomotso P
authors.initials: KP
authors.lastname: Sibeko
authors.forename: Estelle H
authors.initials: EH
authors.lastname: Venter
authors.forename: Moritz
authors.initials: M
authors.lastname: van Vuuren
mesh_terms: D000349:Africa; D000818:Animals; D004283:Dog Diseases / Q000821:virology*; D004285:Dogs; D005814:Genes, Viral / Q000235:genetics; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010322:Parvoviridae Infections / Q000662:veterinary* / Q000821:virology; D017993:Parvovirus, Canine / Q000145:classification / Q000235:genetics*; D010802:Phylogeny