title: Immunosenescent CD8+ T cells and C-X-C chemokine receptor type 3 chemokines are increased in human hypertension.
abstract: The pathogenic role of T cells in hypertension has been documented well in recent animal studies. However, the existence of T-cell-driven inflammation in human hypertension has not been confirmed. Therefore, we undertook immunologic characterization of T cells from patients with hypertension and measured circulating levels of C-X-C chemokine receptor type 3 chemokines, which are well-known tissue-homing chemokines for T cells. We analyzed immunologic markers on T cells from patients with hypertension by multicolor flow cytometry. We then measured circulating levels of the C-X-C chemokine receptor type 3 chemokines, monokine induced by γ interferon (IFN), IFN γ-induced protein 10, and IFN-inducible T-cell α chemoattractant, in patients with hypertension and in age- and sex-matched control subjects by the cytometric bead array method. In addition, we examined histological features of IFN-inducible T-cell α chemoattractant expression from renal biopsy specimens of patients with hypertensive nephrosclerosis and control subjects. The total T-cell population from patients with hypertension showed an increased fraction of immunosenescent, proinflammatory, cytotoxic CD8(+) T cells. Circulating levels of C-X-C chemokine receptor type 3 chemokines were significantly higher in patients with hypertension than in control subjects. Furthermore, immunohistochemical staining revealed increased expression of the T-cell chemokine, IFN-inducible T-cell α chemoattractant, in the proximal and distal tubules of patients with hypertensive nephrosclerosis. Immunosenescent CD8(+) T cells and C-X-C chemokine receptor type 3 chemokines are increased in human hypertension, suggesting a role for T-cell-driven inflammation in hypertension. A more detailed characterization of CD8(+) T cells may offer new opportunities for the prevention and treatment of human hypertension.
authors.affiliation: Cardiology Division, Severance Cardiovascular Hospital, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Jong-Chan
authors.initials: JC
authors.lastname: Youn
authors.forename: Hee Tae
authors.initials: HT
authors.lastname: Yu
authors.forename: Beom Jin
authors.initials: BJ
authors.lastname: Lim
authors.forename: Myoung Ju
authors.initials: MJ
authors.lastname: Koh
authors.forename: Jino
authors.initials: J
authors.lastname: Lee
authors.forename: Dong-Yeop
authors.initials: DY
authors.lastname: Chang
authors.forename: Yoon Seok
authors.initials: YS
authors.lastname: Choi
authors.forename: Sang-Hak
authors.initials: SH
authors.lastname: Lee
authors.forename: Seok-Min
authors.initials: SM
authors.lastname: Kang
authors.forename: Yangsoo
authors.initials: Y
authors.lastname: Jang
authors.forename: Ook Joon
authors.initials: OJ
authors.lastname: Yoo
authors.forename: Eui-Cheol
authors.initials: EC
authors.lastname: Shin
authors.forename: Sungha
authors.initials: S
authors.lastname: Park
mesh_terms: D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D006973:Hypertension / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D007111:Immunity, Cellular; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D011446:Prospective Studies; D054367:Receptors, CXCR3 / Q000096:biosynthesis*
chemical_list: D054367:Receptors, CXCR3
keywords: T cell; aging; chemokine; hypertension; inflammation