title: Baicalin protects rat brain microvascular endothelial cells injured by oxygen-glucose deprivation via anti-inflammation.
abstract: Baicalin, which is isolated from Scutellariae Radix, has been evidenced to possess several pharmacological effects. The present study focuses on the in vitro protective effect of baicalin on oxygen-glucose deprivation (OGD) injured brain microvascular endothelial cells (BMECs) via anti-inflammation and mechanisms against BMECs damaged by OGD. Cultured primary rat BMECs were exposed to baicalin at the concentrations of 100μM (high dose) and 10μM (low dose) for 6h after a 2h OGD. The effects of baicalin were evaluated in terms of (i) cell viability; (ii) lactate dehydrogenase (LDH) leakage rate; (iii) levels of TNF-α, IL-1β, IL-6 in culture media; (iv) protein expressions of p-MEK6, p-MEK1/2, p-ERK, p-IκBα, NF-κB p65, p-IKKα, p-IKKβ and p-p38; and (v) nuclear translocation of NF-κB p65 and p-IκBα. The results showed that OGD treatment could reduce cell viability, increase LDH leakage rate, increase the levels of TNF-α, IL-1β and IL-6 in the culture media. These effects were suppressed by baicalin with high or low dose. In addition, baicalin could notably down-regulate the phosphorylation of proteins in MAPK signaling pathway such as p-MRK1/2, p-ERK and p-p38. While low dose of baicalin could significantly suppress the phosphorylation of proteins in NF-кB signaling pathway such as p-IKKα, p-IKKβ and p-IκBα. Furthermore, baicalin at 10μM could remarkably inhibit nuclear transcriptional activity triggered via NF-κB p65 and p-IκBα in BMECs. In conclusion, baicalin displays a protective effect on OGD-injured BMECs in vitro by attenuating inflammatory factors via down-regulated the MAPK and NF-κB signaling pathway.
authors.affiliation: Xiyuan Hospital of China Academy of Chinese Medical Sciences, 1 Xiyuan Caochang, Hai Dian District, Beijing 100091, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.forename: Jincai
authors.initials: J
authors.lastname: Hou
authors.forename: Jianhua
authors.initials: J
authors.lastname: Fu
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Cuixiang
authors.initials: C
authors.lastname: Zhang
authors.forename: Jianxun
authors.initials: J
authors.lastname: Liu
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001921:Brain / Q000166:cytology / Q000187:drug effects; D002478:Cells, Cultured; D019610:Cytoprotection; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D005419:Flavonoids / Q000494:pharmacology*; D005947:Glucose / Q000172:deficiency; D047908:Intracellular Signaling Peptides and Proteins / Q000187:drug effects / Q000378:metabolism; D055806:Microvessels / Q000166:cytology / Q000187:drug effects; D010100:Oxygen / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D005419:Flavonoids; D047908:Intracellular Signaling Peptides and Proteins; C038044:baicalin; D005947:Glucose; D010100:Oxygen
keywords: Baicalin; Brain microvascular endothelial cells; Inflammation; NF-κB