title: Electrospun biodegradable calcium containing poly(ester-urethane)urea: synthesis, fabrication, in vitro degradation, and biocompatibility evaluation.
abstract: In this work an in vitro degradable poly(ester-urethane)urea (PEUU) was synthesized using polycaprolactone diol, hexamethylene diisocyanate, and calcium salt of p-aminobenzoic acid. The synthesized polymer was characterized by (1) H-NMR and FTIR spectroscopy and viscosity studies. Scaffolds having random micro fibrous structures were fabricated from PEUU by electrospinning process. The thermal properties of the scaffold were evaluated by thermogravimetric analysis and dynamic mechanical analysis. The mechanical property evaluation showed that the scaffold possess sufficiently high tensile strength of 16 MPa. The in vitro degradation studies of the electrospun scaffold were carried out in phosphate buffer saline for 6 months. The average mass loss of the scaffold after 6 months of hydrolytic degradation was 25%. FTIR spectroscopy study confirmed the degradation of the PEUU from decrease in intensity of 1400 cm(-1) peak corresponding to ionic carboxylate group. Presence of amine group and calcium ions in the degradation medium further confirmed the degradation of the hard segment in the hydrolytic medium. The mechanical property evaluation of the scaffold indicated a gradual decrease in tensile strength and modulus whereas percentage elongation of the scaffold increases with time of in vitro degradation. The morphological evaluation of the scaffold after degradation by SEM shows evidence of broken fibers and pores in the scaffold. Preliminary in vitro cytotoxicity test demonstrated that both the material and the degradation products were noncytotoxic in nature and the material showed good proliferation to L-929 cells.
authors.affiliation: Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Poojapura, Thiruvananthapuram-695 012, Kerala, India.
authors.forename: Priya A
authors.initials: PA
authors.lastname: Nair
authors.forename: P
authors.initials: P
authors.lastname: Ramesh
mesh_terms: D020341:Absorbable Implants; D000042:Absorption; D000588:Amines / Q000032:analysis / Q000737:chemistry; D000818:Animals; D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000633:toxicity; D002118:Calcium / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D020724:Elastomers; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008970:Molecular Weight; D011140:Polyurethanes / Q000737:chemistry* / Q000633:toxicity; D017550:Spectroscopy, Fourier Transform Infrared; D013778:Tetrazolium Salts; D013844:Thiazoles; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014867:Water
chemical_list: D000588:Amines; D001672:Biocompatible Materials; D020724:Elastomers; D007202:Indicators and Reagents; D011140:Polyurethanes; D013778:Tetrazolium Salts; D013844:Thiazoles; C501846:poly(ether ester urethane)urea; D014867:Water; C022616:thiazolyl blue; D002118:Calcium