title: The interaction of CuS and Halothiobacillus HT1 biofilm in microscale using synchrotron radiation-based techniques.
abstract: In order to investigate the microbe-mineral interaction in the micro scale, spatial distribution and speciation of Cu and S in Halothiobacillus HT1 biofilm formed on a CuS surface was examined using synchrotron-based X-ray techniques. Confocal laser scanning microscope (CLSM) results indicated that Halothiobacillus HT1 biofilm formation gave rise to distinct chemical and redox gradients, leading to diverse niches in the biofilm. Live cells were distributed at the air-biofilm and membrane-biofilm interface. CuS was oxidized by Halothiobacillus HT1 biofilm, and copper penetrated into the biofilm. Sulfide was oxidized to cysteine (77.3%), sulfite (3.8%) and sulfonate (18.9%). Cu-cysteine-like species were involved in the copper homeostasis. These results significantly improve our understanding of the interfacial properties of the biofilm-mineral interface.
authors.affiliation: Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, China. shijiyan@zju.edu.cn.
authors.forename: Huirong
authors.initials: H
authors.lastname: Lin
authors.forename: Guangcun
authors.initials: G
authors.lastname: Chen
authors.forename: Shenhai
authors.initials: S
authors.lastname: Zhu
authors.forename: Yingxu
authors.initials: Y
authors.lastname: Chen
authors.forename: Dongliang
authors.initials: D
authors.lastname: Chen
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Xiaohan
authors.initials: X
authors.lastname: Yu
authors.forename: Jiyan
authors.initials: J
authors.lastname: Shi
mesh_terms: D018441:Biofilms / Q000187:drug effects*; D003300:Copper / Q000494:pharmacology*; D044222:Halothiobacillus / Q000166:cytology* / Q000187:drug effects / Q000502:physiology*; D050296:Microbial Viability / Q000187:drug effects; D008856:Microscopy, Fluorescence; D013455:Sulfur / Q000302:isolation & purification; D017356:Synchrotrons; D056928:X-Ray Absorption Spectroscopy; D014965:X-Rays
chemical_list: D013455:Sulfur; D003300:Copper; C017846:cupric sulfide