title: In vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cells.
abstract: OBJECTIVE
  To evaluate in vitro ability the of three different biomaterials - purified hydroxyapatite, demineralized bone matrix and castor oil-based polyurethane - as biocompatible 3D scaffolds for canine bone marrow mesenchymal stem cell (MSC) intending bone tissue engineering.
  
  
  METHODS
  MSCs were isolated from canine bone marrow, characterized and cultivated for seven days with the biomaterials. Cell proliferation and adhesion to the biomaterial surface were evaluated by scanning electron microscopy while differentiation into osteogenic lineage was evaluated by Alizarin Red staining and Sp7/Osterix surface antibody marker.
  
  
  RESULTS
  The biomaterials allowed cellular growth, attachment and proliferation. Osteogenic differentiation occurred in the presence of hydroxyapatite, and matrix deposition commenced in the presence of the castor oil-based polyurethane.
  
  
  CONCLUSIONS
  All the tested biomaterials may be used as mesenchymal stem cell scaffolds in cell-based orthopedic reconstructive therapy.
authors.affiliation: Department of Veterinary Surgery and Anesthesiology, School of Veterinary Medicine and Animal Science, São Paulo State University, Botucatu, SP, Brazil.
authors.forename: Oduvaldo Câmara Marques
authors.initials: OC
authors.lastname: Pereira-Junior
authors.forename: Sheila Canevese
authors.initials: SC
authors.lastname: Rahal
authors.forename: João Ferreira
authors.initials: JF
authors.lastname: Lima-Neto
authors.forename: Fernanda da Cruz
authors.initials: Fda C
authors.lastname: Landim-Alvarenga
authors.forename: Frederico Ozanan Barros
authors.initials: FO
authors.lastname: Monteiro
mesh_terms: D000818:Animals; D001857:Bone Matrix; D001861:Bone Regeneration / Q000187:drug effects; D018786:Bone Substitutes / Q000627:therapeutic use*; D002368:Castor Oil / Q000627:therapeutic use*; D002448:Cell Adhesion; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004285:Dogs; D017886:Durapatite / Q000627:therapeutic use*; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000187:drug effects*; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis / Q000187:drug effects; D011140:Polyurethanes / Q000627:therapeutic use*; D013499:Surface Properties; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D018786:Bone Substitutes; D011140:Polyurethanes; D002368:Castor Oil; D017886:Durapatite