title: Identification of prostate cancer-associated microRNAs in circulation using a mouse model of disease.
abstract: MicroRNAs (miRNAs) derived from the cell-free fractions of blood are emerging as useful noninvasive markers of cancer. However, many tumors display significant molecular heterogeneity, which is likely to be reflected in the circulating miRNA fingerprints associated with that pathology. One strategy to minimize such heterogeneity is to employ genetically engineered mouse models of human cancer. Here, we describe a method to profile miRNAs in the serum of a mouse model of prostate cancer, TRansgenic Adenocarcinoma of Mouse Prostate (TRAMP), and discuss practical considerations for translating these potential biomarkers into a clinical setting.
authors.affiliation: Dame Roma Mitchell Cancer Research Laboratories, The University of Adelaide, Adelaide, SA, Australia.
authors.forename: Luke A
authors.initials: LA
authors.lastname: Selth
authors.forename: Scott L
authors.initials: SL
authors.lastname: Townley
authors.forename: Joanna L
authors.initials: JL
authors.lastname: Gillis
authors.forename: Wayne D
authors.initials: WD
authors.lastname: Tilley
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Butler
mesh_terms: D000230:Adenocarcinoma / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D000818:Animals; D014408:Biomarkers, Tumor / Q000097:blood*; D004195:Disease Models, Animal; D018450:Disease Progression; D006801:Humans; D008297:Male; D051379:Mice; D035683:MicroRNAs / Q000097:blood*; D020411:Oligonucleotide Array Sequence Analysis; D011471:Prostatic Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000592:standards*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000592:standards*
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs