title: [Determination of chlorogenic acid and baicalin in Yinhuang film-coated tablets by HPLC isocratic elution].
abstract: OBJECTIVE
  To establish an HPLC method for determination of chlorogenic acid and baicalin in Yinhuang film-coated tablets.
  
  
  METHODS
  HPLC isocratic elution was adopted, with the C18 column (4.6 mm x 250 mm, 5 microm) as the chromatographic column, methanol-water-phosphoric acid (45: 55: 0.2) as the mobile phase. The flow rate was set at 1.0 mL x min(-1), and column temperature was set at 30 degrees C. The detection wavelength was 328 nm, and the sample size was 20 microL.
  
  
  RESULTS
  At concentrations ranging between 2.200-44.00, 24.20-242.0 mg x L(-1), peak area of chlorogenic acid and baicalin showed good linear relationship, the average recoveries were 99.63% (RSD 1.8%, n = 9) and 99.72% (RSD 1.9%, n = 9), respectively.
  
  
  CONCLUSIONS
  The method is simple that it can accurately measure the content of chlorogenic acid and baicalin in Yinhuang film-coated tablets.
authors.affiliation: Department of Pharmaceutical Analysis, College of Pharmacy, Henan University, Kaifeng 475001, China. kf_xm@yahoo.com.cn
authors.forename: Mei
authors.initials: M
authors.lastname: Xu
authors.forename: Qi
authors.initials: Q
authors.lastname: Yuan
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.forename: Fan
authors.initials: F
authors.lastname: Li
authors.forename: Xiao-Lei
authors.initials: XL
authors.lastname: Hu
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Fu
authors.forename: Guo-Hua
authors.initials: GH
authors.lastname: Shi
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Wang
authors.forename: Na
authors.initials: N
authors.lastname: Zhang
mesh_terms: D002726:Chlorogenic Acid / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry; D016014:Linear Models; D013607:Tablets
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D013607:Tablets; D002726:Chlorogenic Acid; C038044:baicalin