title: Chemical activation of RARβ induces post-embryonically bilateral limb duplication during Xenopus limb regeneration.
abstract: The anuran amphibian Xenopus laevis can regenerate its limbs for a limited time during the larval stage, while limbs are still developing. Using this regeneration model, we evaluated the proximo-distal blastema cell identity when endogenous retinoids were increased by CYP26 inhibition or when RAR-specific agonists altered RA signaling. Simultaneous proximo-distal and antero-posterior limb duplications were generated, and the RAR-specific agonist can modify blastema identity after amputation, because chemical activation of RARβ produced bilateral hindlimb duplications that resulted in a drastic duplication phenotype of regenerating limbs.
authors.affiliation: Laboratorio de Evolución y Embriología, Facultad de Ciencias Biológicas y Agropecuarias, Universidad Veracruzana. Carretera Tuxpan-Tampico Km 7.5 C.P. 92860, Tuxpan, Ver. MÉXICO. rodcuervo@uv.mx
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Cuervo
authors.forename: Jesús
authors.initials: J
authors.lastname: Chimal-Monroy
mesh_terms: D000818:Animals; D001565:Benzoates / Q000494:pharmacology*; D052160:Benzothiazoles / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000494:pharmacology*; D065607:Cytochrome P-450 Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D005121:Extremities / Q000196:embryology / Q000254:growth & development*; D007814:Larva / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D018168:Receptors, Retinoic Acid / Q000819:agonists / Q000378:metabolism*; D012038:Regeneration / Q000187:drug effects* / Q000502:physiology; D000072516:Retinoic Acid 4-Hydroxylase; D012176:Retinoids / Q000494:pharmacology; D014230:Triazoles / Q000494:pharmacology*; D014982:Xenopus laevis / Q000196:embryology / Q000254:growth & development / Q000378:metabolism
chemical_list: C507710:4'-octyl-4-biphenylcarboxylic acid; D001565:Benzoates; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D065607:Cytochrome P-450 Enzyme Inhibitors; D018168:Receptors, Retinoic Acid; D012176:Retinoids; D014230:Triazoles; D003577:Cytochrome P-450 Enzyme System; D000072516:Retinoic Acid 4-Hydroxylase; C406527:R 115866