title: Super-resolution fluorescence imaging of directly labelled DNA: from microscopy standards to living cells.
abstract: Super-resolution fluorescence microscopy is ideally suited to study the complex organization of cell DNA in the 10-100 nm range. Novel methods to image directly labelled DNA, instead of DNA-associated proteins, are being developed and refined. This minireview provides an update of recent progress in super-resolution fluorescence imaging methods for DNA. These developments should allow a deeper understanding of chromatin structure and widen the scope of biological processes that may be investigated with super-resolution fluorescence microscopy.
authors.affiliation: Madrid Institute for Advanced Studies in Nanoscience (IMDEA Nanoscience), Madrid, Spain. cristina.flors@imdea.org
authors.forename: C
authors.initials: C
authors.lastname: Flors
mesh_terms: D004247:DNA / Q000378:metabolism* / Q000648:ultrastructure*; D005456:Fluorescent Dyes / Q000378:metabolism*; D008856:Microscopy, Fluorescence / Q000379:methods*; D061848:Optical Imaging / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D004247:DNA