title: Catechin attenuates behavioral neurotoxicity induced by 6-OHDA in rats.
abstract: This study was designed to investigate the beneficial effect of catechin in a model of Parkinson's disease. Unilateral, intrastriatal 6-hydroxydopamine (6-OHDA)-lesioned rats were pretreated with catechin (10 and 30 mg/kg) by intraperitoneal (i.p.) injection 2h before surgery and for 14 days afterwards. After treatments, apomorphine-induced rotations, locomotor activity, working memory and early and late aversive memories were evaluated. The mesencephalon was used to determine the levels of monoamines and measurement of glutathione (GSH). Immunohistochemical staining was also used to evaluate the expression of tyrosine hydroxylase (TH) in mesencephalic and striatal tissues. Catechin administration attenuated the increase in rotational behavior and the decrease in locomotor activity observed in lesioned rats. Although catechin did not rescue the impairment of late aversive memory, it protected the animals against 6-OHDA-induced working memory deficits. Furthermore, catechin treatment restored GSH levels, and significantly increased dopamine and DOPAC content, and TH-immunoreactivity in 6-OHDA-lesioned rats. Catechin protected 6-OHDA-lesioned rats due to its antioxidant action, indicating that it could be useful as an adjunctive therapy for the treatment of Parkinson's disease.
authors.affiliation: Laboratory of Neurosciences and Behavior, Federal University of Ceará, Rua Cel. Nunes de Melo, 1127, Fortaleza 60430270, Brazil.
authors.forename: M D A
authors.initials: MD
authors.lastname: Teixeira
authors.forename: C M
authors.initials: CM
authors.lastname: Souza
authors.forename: A P F
authors.initials: AP
authors.lastname: Menezes
authors.forename: M R S
authors.initials: MR
authors.lastname: Carmo
authors.forename: A A
authors.initials: AA
authors.lastname: Fonteles
authors.forename: J P
authors.initials: JP
authors.lastname: Gurgel
authors.forename: F A V
authors.initials: FA
authors.lastname: Lima
authors.forename: G S B
authors.initials: GS
authors.lastname: Viana
authors.forename: G M
authors.initials: GM
authors.lastname: Andrade
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D002392:Catechin / Q000494:pharmacology*; D003342:Corpus Striatum / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D008297:Male; D008568:Memory / Q000187:drug effects; D008636:Mesencephalon / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D016627:Oxidopamine / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
chemical_list: D016627:Oxidopamine; D002392:Catechin; D014446:Tyrosine 3-Monooxygenase; D005978:Glutathione
keywords: 6-OHDA; Catechin; GSH; Memory; Parkinson's disease