title: Functional analysis of exosomal microRNA in cell-cell communication research.
abstract: Circulating microRNAs (miRNAs), also known as secretory miRNAs, are packaged in small membrane vesicles called exosomes. These exosomal miRNAs are secreted from various cell types and incorporated inside the recipient cells. The functions of exosomal miRNAs are poorly understood, but some reports have shown their essential roles in cancer development. Therefore, methods to study the function of exosomal miRNAs not only in vitro but also in vivo might be essential. We have analyzed the function of exosomal miRNAs by miRNA-enriched exosomes both in vitro and in vivo. In this chapter, the methods to concentrate the targeted miRNAs are provided. This simple and useful method enables the study of the precise mechanisms of exosomal miRNAs under physiological and pathological conditions.
authors.affiliation: Division of Molecular and Cellular Medicine, National Cancer Center Research Institute, Tokyo, Japan.
authors.forename: Nobuyoshi
authors.initials: N
authors.lastname: Kosaka
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Yoshioka
authors.forename: Keitaro
authors.initials: K
authors.lastname: Hagiwara
authors.forename: Naoomi
authors.initials: N
authors.lastname: Tominaga
authors.forename: Takahiro
authors.initials: T
authors.lastname: Ochiya
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000737:chemistry* / Q000473:pathology; D019556:COS Cells; D002450:Cell Communication; D002458:Cell Fractionation; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D055354:Exosomes / Q000737:chemistry* / Q000235:genetics; D015870:Gene Expression; D057809:HEK293 Cells; D006801:Humans; D015552:Injections, Intralesional; D008297:Male; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000302:isolation & purification*; D009368:Neoplasm Transplantation; D011471:Prostatic Neoplasms / Q000737:chemistry* / Q000473:pathology; D014461:Ultracentrifugation
chemical_list: D035683:MicroRNAs