title: Simultaneous determination of 5 major bioactive components in hugu capsule by HPLC-DAD.
abstract: OBJECTIVE
  To develop a qualitative and quantitative determination of multiple components for quality control of Hugu Capsule, a composite prescription in TCM.
  
  
  METHODS
  HPLC analysis was performed on a Kromasil C18 column (5.0 microm, 250 mm x 4.6 mm) by gradient elution with methanol-acetonitrile-1% glacial acetic acid as the mobile phase at a flow-rate of 0.8 mL/min,the detection wavelengths were set at 270, 283, 320 and 325 nm at the temperature of 30 degrees C.
  
  
  RESULTS
  Five components: chlorogenic acid, tetrahydroxy stilbene glucoside, ferulaic acid, naringin and icariin were simultaneously analyzed in this study. The calibration curves were exhibited linear regressions of at least r > 0.9992. The injection precision,the intra-day precisions and the analysis repeatability were evaluated with the RSD values were all less than 5%. The mean recoveries of the five components were ranged from 97.4% to 99.4%, and RSD values all were less than 1.72%.
  
  
  CONCLUSIONS
  This method is found to be convenient, fast, accurate, and is applicable to analyze the multi-constituents in Hugu Capsule.
authors.affiliation: School of Pharmacy, Guangdong Medical College, Dongguan 523808, China.
authors.forename: Li-Jun
authors.initials: LJ
authors.lastname: Song
authors.forename: Wen-Chang
authors.initials: WC
authors.lastname: Zhao
authors.forename: Qiu-Ling
authors.initials: QL
authors.lastname: Zeng
authors.forename: Jin-Dong
authors.initials: JD
authors.lastname: Wu
authors.forename: Bao-Hong
authors.initials: BH
authors.lastname: Li
mesh_terms: D002214:Capsules; D002726:Chlorogenic Acid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000032:analysis; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D044950:Flavanones / Q000032:analysis*; D005419:Flavonoids / Q000032:analysis; D005960:Glucosides / Q000032:analysis*; D011786:Quality Control; D015203:Reproducibility of Results; D013267:Stilbenes / Q000032:analysis*
chemical_list: D002214:Capsules; D003373:Coumaric Acids; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D005419:Flavonoids; D005960:Glucosides; D013267:Stilbenes; C093026:2,3,5,4'-tetrahydroxystilbene 2-O-glucopyranoside; D002726:Chlorogenic Acid; C004999:ferulic acid; C005274:naringin; C056599:icariin