title: Activation profile of dorsal root ganglia Iba-1 (+) macrophages varies with the type of lesion in rats.
abstract: The interactions between neurons, immune and immune-like glial cells can initiate the abnormal processes that underlie neuropathic pain. In the peripheral nervous system the resident macrophages may play an important role. In this study we investigated in experimental adult Sprague-Dawley rats how Iba-1 (ionized calcium binding adaptor molecule 1) (+) resident macrophages in the dorsal root ganglion (DRG) are activated after a spinal nerve ligation (SNL) or streptozotocin (STZ)-induced diabetes. The activation profile was defined by comparing the responses of resident macrophages against microglia in the spinal cord as they share a common origin. After SNL, the Iba-1 (+) macrophages in L5 DRG reached their activation peak 5 days later, clustered as satellite cells around large A-neurons, expressed the MHC-II marker, but did not show p-p38 and p-ERK1/2 activation and did not secrete IL-18. After STZ-induced diabetes, the Iba-1 (+) macrophages reached their activation peak 1 week later in L4 and L5 DRG, remained scattered between neurons, expressed the MHC-II marker only in L5 DRG, did not show p-p38 and p-ERK1/2 activation and did not secrete any of the investigated cytokines/chemokines. These responses suggest that depending on the type of lesion DRG Iba-1 (+) resident macrophages have different activation mechanisms, which are dissimilar to those in microglia.
authors.affiliation: Department of Anatomy, Physiology and Biophysics, Faculty of Biology, University of Bucharest, Splaiul Independenţei 91-95, 050095 Bucharest, Romania; Department of Anatomy and Physiology, Faculty of Biology, Vinh University, 182 Le Duan, Nghe An, Vietnam.
authors.forename: Bich-Hoai Thi
authors.initials: BH
authors.lastname: Ton
authors.forename: Qingmin
authors.initials: Q
authors.lastname: Chen
authors.forename: Gisela
authors.initials: G
authors.lastname: Gaina
authors.forename: Catalin
authors.initials: C
authors.lastname: Tucureanu
authors.forename: Adriana
authors.initials: A
authors.lastname: Georgescu
authors.forename: Carmen
authors.initials: C
authors.lastname: Strungaru
authors.forename: Maria-Luiza
authors.initials: ML
authors.lastname: Flonta
authors.forename: Dinah
authors.initials: D
authors.lastname: Sah
authors.forename: Violeta
authors.initials: V
authors.lastname: Ristoiu
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D005727:Ganglia, Spinal / Q000378:metabolism* / Q000473:pathology; D008264:Macrophages / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008840:Microfilament Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: C100745:Aif1 protein, rat; D002135:Calcium-Binding Proteins; D008840:Microfilament Proteins
keywords: Diabetic neuropathy; Dorsal root ganglia; Iba-1; Macrophages; Neuropathic pain; Rat; Spinal nerve ligation