title: Isolation of immunoglobulin G from bovine milk whey by poly(hydroxyethyl methacrylate)-based anion-exchange cryogel.
abstract: Bovine milk whey contains several bioactive proteins such as α-lactalbumin, β-lactoglobulin, and immunoglobulin G (IgG). Chromatographic separation of these proteins has received much attention in the past few years. In this work, we provide a chromatographic method for the efficient isolation of IgG from bovine milk whey using a poly(2-hydroxyethyl methacrylate)-based anion-exchange cryogel. The monolithic cryogel was prepared by grafting 2-(dimethylamino) ethyl methacrylate onto the poly(2-hydroxyethyl methacrylate)-based cryogel matrix and then employed to separate IgG under various buffer pH and salt elution conditions. The results showed that the buffer pH and the salt concentration in the step elution have remarkable influences on the purity of IgG, while the IgG recovery depended mainly on the loading volume of whey for a given cryogel bed. High purity IgG (more than 95%) was obtained using the phosphate buffer with pH of 5.8 as the running buffer and the salt solution in as the elution liquid. With suitable loading volume of whey, the maximum IgG recovery of about 94% was observed. The present separation method is thus a potential choice for the isolation of high-purity IgG from bovine milk whey.
authors.affiliation: Key Laboratory for Green Processing of Chemical Engineering of the Xinjiang Bingtuan, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi, PR China.
authors.forename: Shasha
authors.initials: S
authors.lastname: Dong
authors.forename: Liang
authors.initials: L
authors.lastname: Chen
authors.forename: Bin
authors.initials: B
authors.lastname: Dai
authors.forename: Wilfred
authors.initials: W
authors.lastname: Johnson
authors.forename: Jialei
authors.initials: J
authors.lastname: Ye
authors.forename: Shaochuan
authors.initials: S
authors.lastname: Shen
authors.forename: Junxian
authors.initials: J
authors.lastname: Yun
authors.forename: Kejian
authors.initials: K
authors.lastname: Yao
authors.forename: Dong-Qiang
authors.initials: DQ
authors.lastname: Lin
authors.forename: Shan-Jing
authors.initials: SJ
authors.lastname: Yao
mesh_terms: D000818:Animals; D000838:Anions / Q000737:chemistry; D002417:Cattle; D002852:Chromatography, Ion Exchange; D059985:Cryogels / Q000737:chemistry*; D007074:Immunoglobulin G / Q000302:isolation & purification*; D008894:Milk Proteins / Q000737:chemistry*; D011102:Polyhydroxyethyl Methacrylate / Q000737:chemistry*; D000067816:Whey Proteins
chemical_list: D000838:Anions; D059985:Cryogels; D007074:Immunoglobulin G; D008894:Milk Proteins; D000067816:Whey Proteins; D011102:Polyhydroxyethyl Methacrylate
keywords: Anion exchange; Cryogel; Immunoglobulin G; Milk whey; Monoliths