title: Molecular cloning, characterization and expression analysis of coagulation factor VII gene in grass carp (Ctenopharyngodon idella).
abstract: Coagulation factor VII has been studied in several species but, to date, not in grass carp (Ctenopharyngodon idella), a commercially important freshwater fish found in China. In this study, the full-length cDNA of grass carp coagulation factor VII (GcCFVII) was cloned using a RACE-Ready cDNA Kit, grass carp were challenged with a hemorrhagic virus, and temporal expression profiles of GcCFVII in the thymus, gills, liver, spleen, and head kidney were examined at 0 h, 24 h, 48 h, 72 h, 96 h, and 138 h using fluorescence quantitative PCR. The results showed the 1480 bp GcCFVII to contain three conservative motifs: Gla, EGF-CA, and Tryp-SPc, similar to other species. Phylogenetic analysis showed the evolution of GcCFVII gene to be consistent with the evolution of the species. After viral challenge, GcCFVII expression in five tissues of grass carp showed different patterns of fluctuation. These results provide a solid basis for further investigation of GcCFVII and its relationship with grass carp hemorrhage.
authors.affiliation: College of Animal Science and Technology, Hunan Agricultural University, Nongda Road 1, Changsha 410128, China.
authors.forename: Qiaolin
authors.initials: Q
authors.lastname: Liu
authors.forename: Baohong
authors.initials: B
authors.lastname: Xu
authors.forename: Tiaoyi
authors.initials: T
authors.lastname: Xiao
authors.forename: Jianming
authors.initials: J
authors.lastname: Su
authors.forename: Lei
authors.initials: L
authors.lastname: Zhong
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000235:genetics* / Q000276:immunology* / Q000378:metabolism; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D005167:Factor VII / Q000235:genetics* / Q000378:metabolism; D029941:Fish Proteins / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D008969:Molecular Sequence Data; D009928:Organ Specificity; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D017434:Protein Structure, Tertiary; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012087:Reoviridae / Q000502:physiology; D017386:Sequence Homology, Amino Acid
chemical_list: D018076:DNA, Complementary; D029941:Fish Proteins; D012333:RNA, Messenger; D005167:Factor VII
keywords: Coagulation; Conservative motif; Factor VII; Grass carp; Grass carp hemorrhagic virus (GCHV)