title: [Dynamic monitoring and analysis of the persisters emerging in Candida albicans biofilm].
abstract: OBJECTIVE
  To investigate the mechanism and approach related to persisters emerging in Candida albicans biofilm by studying its dynamic characteristics.
  
  
  METHODS
  The Candida albicans biofilms model in vitro were formed by individual yeast cells and hyphal compartments respectively. The numbers of progenitive fungal cells and persisters at different stages were counted and analyzed with SPSS11.5 software package. The biofilm morphological changes were observed under confocal scanning laser microscopy.
  
  
  RESULTS
  Different stages of biofilms which were formed by individual yeast cells and hyphal compartments had no significant difference both on the number of progenitive fungal cells and persisters. The number of progenitive fungal cells showed S-shaped growth curve until 12 hours. The persisters emerged within 0.5 hour and its number reached a steady state after 2 hours while the biofilm was in the period of microcolony formation under microscopy.
  
  
  CONCLUSIONS
  The persisters emerging in Candida albicans biofilm are closely connected with the attachment to a surface(within 2 hours of the biofilm formation) and have little association with the maturity of the biofilm and its dimorphism.
authors.affiliation: Department of Endodontics, Shandong University, Key Lab of Oral Biomedicine of Shandong Province, Shandong Province, China.
authors.forename: Hong-nan
authors.initials: HN
authors.lastname: Dong
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xia
authors.initials: X
authors.lastname: Wu
authors.forename: Hao-yue
authors.initials: HY
authors.lastname: Chu
authors.forename: Qing-guo
authors.initials: QG
authors.lastname: Qi
mesh_terms: D018441:Biofilms; D002176:Candida albicans; D006801:Humans; D025301:Hyphae; D008853:Microscopy; D018613:Microscopy, Confocal