title: Mitochondrial localization of ferrochelatase in a red alga Cyanidioschyzon merolae.
abstract: Ferrochelatase (FECH) is an essential enzyme for the final step of heme biosynthesis. In green plants, its activity has been reported in both plastids and mitochondria. However, the precise subcellular localization of FECH remains uncertain. In this study, we analyzed the localization of FECH in the unicellular red alga, Cyanidioschyzon merolae. Immunoblot and enzyme activity analyses of subcellular fractions localized little FECH in the plastid. In addition, immunofluorescence microscopy identified that both intrinsic and hemagglutinin (HA)-tagged FECH are localized in the mitochondrion. We therefore conclude that FECH is localized in the mitochondrion in C. merolae.
authors.affiliation: Department of Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Watanabe
authors.forename: Mitsumasa
authors.initials: M
authors.lastname: Hanaoka
authors.forename: Yusaku
authors.initials: Y
authors.lastname: Ohba
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Ono
authors.forename: Mio
authors.initials: M
authors.lastname: Ohnuma
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Yoshikawa
authors.forename: Shigeru
authors.initials: S
authors.lastname: Taketani
authors.forename: Kan
authors.initials: K
authors.lastname: Tanaka
mesh_terms: D020418:Algal Proteins / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D005294:Ferrochelatase / Q000235:genetics / Q000378:metabolism*; D007106:Immune Sera; D051379:Mice; D008856:Microscopy, Fluorescence; D008928:Mitochondria / Q000201:enzymology*; D011993:Recombinant Fusion Proteins; D000461:Rhodophyta / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D045725:Tetrapyrroles / Q000378:metabolism
chemical_list: D020418:Algal Proteins; D007106:Immune Sera; D011993:Recombinant Fusion Proteins; D045725:Tetrapyrroles; D005294:Ferrochelatase
keywords: Cyanidioschyzon merolae; Evolution; Ferrochelatase; Tetrapyrrole biosynthesis; Transformation