title: Feruloyl-L-arabinose attenuates migration, invasion and production of reactive oxygen species in H1299 lung cancer cells.
abstract: Ferulic acid (FA), a phenolic compound, is an abundant dietary antioxidant and exerts the mitogenic effect on cells. Recently, we isolated an active FA derivative, namely feruloyl-L-arabinose (FAA), from coba husk. The aim of this study was to investigate the effects of FAA on the proliferation, migration and invasion of H1299 human lung cancer cells. Our results showed a strong antioxidant potential of FAA. Additionally, FAA inhibited the migration and invasion ability, while causing a significant accumulation of G2/M-population, of H1299 tumor cells in a dose-dependent manner, whereas no significant change on cell proliferation was observed. Results from the wound healing assay revealed that cell migration ability was markedly inhibited by FAA treatments. Similarly, results of gelatin zymography study showed that FAA treatments significantly decreased the activities of matrix metalloproteinase (MMP)-2 and MMP-9, suggesting that FAA-mediated inhibition on migration and invasion of lung cancer cells may be achieved by the down-regulation of the MMPs activities. Taken together, our present work provides a new insight into the novel inhibitory function of FAA on cell migration in H1299 cells, suggesting its promising role in the chemoprevention of lung cancer.
authors.affiliation: Department of Food Nutrition, Chung-Hwa University of Medical Technology, Tainan 701, Taiwan.
authors.forename: Hsin-Yu
authors.initials: HY
authors.lastname: Fang
authors.forename: Hui-Min
authors.initials: HM
authors.lastname: Wang
authors.forename: Kuo-Feng
authors.initials: KF
authors.lastname: Chang
authors.forename: Huei-Ting
authors.initials: HT
authors.lastname: Hu
authors.forename: Lian-Je
authors.initials: LJ
authors.lastname: Hwang
authors.forename: Tzu-Fun
authors.initials: TF
authors.lastname: Fu
authors.forename: Yin-Chieh
authors.initials: YC
authors.lastname: Lin
authors.forename: Wei-Chiao
authors.initials: WC
authors.lastname: Chang
authors.forename: Tsu-Pei
authors.initials: TP
authors.lastname: Chiu
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Wen
authors.forename: Yao
authors.initials: Y
authors.lastname: Fong
authors.forename: Chien-Chih
authors.initials: CC
authors.lastname: Chiu
authors.forename: Bing-Hung
authors.initials: BH
authors.lastname: Chen
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D001089:Arabinose / Q000031:analogs & derivatives* / Q000494:pharmacology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D003373:Coumaric Acids / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D016166:Free Radical Scavengers / Q000494:pharmacology; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism / Q000473:pathology*; D009361:Neoplasm Invasiveness / Q000517:prevention & control*; D017382:Reactive Oxygen Species / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000975:Antioxidants; D003373:Coumaric Acids; D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; C586709:feruloylarabinose; D001089:Arabinose
keywords: Antioxidant; Ferulic acid; Feruloyl-l-arabinose; Invasion; Migration; Non-small cell lung cancer