title: Oxidative stress, trace elements, and circulating microparticles in patients with Gaucher disease before and after enzyme replacement therapy.
abstract: We studied the level of lipid peroxide, nitric oxide (NO), trace elements (TEs), and microparticles (MPs) in Gaucher disease (GD) before and after 1 year of enzyme replacement therapy (ERT). A total of 15 children with GD and 15 healthy controls were enrolled in this study. Serum level of lipid peroxide, NO, and TEs was determined. The MPs were detected by flow cytometry. The level of lipid peroxide was significantly higher in the patients than in the controls even after ERT. Although NO level was normalized in the patients after ERT, zinc and copper were still lower in the patients after ERT. The percentages of various MPs were significantly higher in the patients than in the controls both before and after ERT. There were positive correlations between chitotriosidase and both lipid peroxide and total MPs.
  
  
  CONCLUSIONS
  The GD is associated with alteration in oxidant and antioxidant status and high level of circulating MPs.
authors.affiliation: Department of Oncological Clinical Pathology, Flow Cytometry Lap, South Egypt Cancer Institute, Assiut University, Egypt.
authors.forename: Asmaa M
authors.initials: AM
authors.lastname: Zahran
authors.affiliation: Department of Pediatrics, Faculty of Medicine, Assiut University, Egypt elsayh23@yahoo.com.
authors.forename: Khalid I
authors.initials: KI
authors.lastname: Elsayh
authors.affiliation: Department of Medical Biochemistry, Faculty of Medicine, Assiut University, Egypt.
authors.forename: Sahar E M
authors.initials: SE
authors.lastname: El-Deek
authors.affiliation: Department of Medical Biochemistry, Faculty of Medicine, Assiut University, Egypt.
authors.forename: Mona A H
authors.initials: MA
authors.lastname: El-Baz
mesh_terms: D016022:Case-Control Studies; D055252:Cell-Derived Microparticles / Q000187:drug effects; D002648:Child; D002675:Child, Preschool; D003300:Copper / Q000097:blood; D056947:Enzyme Replacement Therapy; D005260:Female; D005776:Gaucher Disease / Q000097:blood* / Q000188:drug therapy*; D005962:Glucosylceramidase / Q000627:therapeutic use*; D006596:Hexosaminidases / Q000097:blood; D006801:Humans; D008054:Lipid Peroxides / Q000097:blood; D008297:Male; D009569:Nitric Oxide / Q000097:blood; D018384:Oxidative Stress / Q000187:drug effects; D012643:Selenium / Q000097:blood; D014131:Trace Elements / Q000097:blood; D015032:Zinc / Q000097:blood
chemical_list: D008054:Lipid Peroxides; D014131:Trace Elements; D009569:Nitric Oxide; D003300:Copper; D006596:Hexosaminidases; C086400:chitotriosidase; D005962:Glucosylceramidase; D012643:Selenium; D015032:Zinc
keywords: Gaucher disease; flow cytometry; microparticles