title: Polysaccharide-rich fraction of Termitomyces eurhizus accelerate healing of indomethacin induced gastric ulcer in mice.
abstract: The current study aims to determine the healing activity of water soluble polysaccharide-rich fraction of a wild mushroom, Termitomyces eurhizus (TEps) against the indomethacin induced gastric ulceration in mice model. Gastric tissue histology, myeloperoxidase (MPO) activity, cyclooxygenases (COX) 1 and 2 expression, prostaglandin E2 (PGE2) synthesis, and modulation of pro/anti inflammatory cytokines expression were studied for this purpose. Histological study shows that TEps (20 mg/kg) effectively healed the gastric ulceration. Based on biochemical results, the healing capacities of TEps could be attributed to reduction of MPO activity and protection of mucosal mucin content. Enhanced synthesis of PGE2 by modulation of COX-1 and COX-2 expression and a prominent shift of cytokines expression from pro (TNF-α, IL-1ß) to anti inflammatory (IL-10) side are also held responsible for ulcer healing. The preliminary study highlights the anti-ulcerogenic property of polysaccharide-rich fraction of Termitomyces eurhizus and opens an alternative cure for NSAID induced gastroduodenal diseases.
authors.affiliation: Central Research Laboratory, Department of Biochemistry, KPC Medical College and Hospital, 1F, Raja S.C. Mullick Road, Jadavpur, Kolkata, 700032, West Bengal, India, ananya.biochem@gmail.com.
authors.forename: Ananya
authors.initials: A
authors.lastname: Chatterjee
authors.forename: Somanjana
authors.initials: S
authors.lastname: Khatua
authors.forename: Sirshendu
authors.initials: S
authors.lastname: Chatterjee
authors.forename: Shatavisa
authors.initials: S
authors.lastname: Mukherjee
authors.forename: Atashi
authors.initials: A
authors.lastname: Mukherjee
authors.forename: Soumitra
authors.initials: S
authors.lastname: Paloi
authors.forename: Krishnendu
authors.initials: K
authors.lastname: Acharya
authors.forename: Sandip K
authors.initials: SK
authors.lastname: Bandyopadhyay
mesh_terms: D000818:Animals; D002457:Cell Extracts / Q000627:therapeutic use*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D015232:Dinoprostone / Q000235:genetics / Q000378:metabolism; D062610:Fungal Polysaccharides / Q000627:therapeutic use*; D007213:Indomethacin / Q000633:toxicity; D051379:Mice; D009077:Mucins / Q000378:metabolism; D009195:Peroxidase / Q000235:genetics / Q000378:metabolism; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics / Q000378:metabolism; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D055434:Termitomyces / Q000737:chemistry*
chemical_list: D002457:Cell Extracts; D016207:Cytokines; D062610:Fungal Polysaccharides; D009077:Mucins; D009195:Peroxidase; D011451:Prostaglandin-Endoperoxide Synthases; D015232:Dinoprostone; D007213:Indomethacin