title: Detection of human cytomegalovirus and Epstein-Barr Virus in symptomatic and asymptomatic apical periodontitis lesions by real-time PCR.
abstract: OBJECTIVE
  Recent studies have investigated the occurrence of human cytomegalovirus and Epstein-Barr Virus in samples from apical periodontitis lesions and a role in the pathogenesis of this disease has been suggested. Because genotype distribution and seroprevalence of EBV and HCMV differ among populations, it is important to determine the presence of these viruses in endodontic periapical lesions of different populations. The aims of this study were to determine the presence of HCMV and EBV DNAs in samples from Turkish patients with symptomatic and asymptomatic apical periodontitis lesions using real-time polymerase chain reaction method and to evaluate their presence in both symptomatic and asymptomatic apical periodontitis lesions.
  
  
  METHODS
  Periapical samples were collected from 12 asymptomatic and 16 symptomatic periapical lesions in conjunction with apicectomy. HCMV and EBV DNAs were identified in the samples by real-time PCR. The chi-squared test with Yates's correction or the Fisher's exact test was used to analyse the significance of differences.
  
  
  RESULTS
  HCMV DNA was detected in 10 of the 16 (62.5%) symptomatic and in five of the 12 (41.7 %) asymptomatic periapical study lesions. The EBV DNA was identified in seven of the 16 (43.7 %) symptomatic and three of the 12 (25 %) asymptomatic periapical lesions. The difference in occurrence of HCMV and EBV DNA between symptomatic and asymptomatic periapical lesions was not statistically significant. (All comparisons have p > 0.05).
  
  
  CONCLUSIONS
  Our findings suggest that HCMV and EBV is a frequent inhabitant of both symptomatic and asymptomatic apical periodontitis lesions of endodontic origin in Turkish population.
authors.affiliation: Department of Microbiology and Clinical Microbiology, Medical Faculty, Sakarya University, TR-54000 Sakarya, Türkiye, ahmetozbek1965@yahoo.com.
authors.forename: Selcuk-M
authors.initials: SM
authors.lastname: Ozbek
authors.forename: Ahmet
authors.initials: A
authors.lastname: Ozbek
authors.forename: Muhammed-Selim
authors.initials: MS
authors.lastname: Yavuz
mesh_terms: D000328:Adult; D058345:Asymptomatic Infections; D003587:Cytomegalovirus / Q000302:isolation & purification*; D004854:Herpesvirus 4, Human / Q000302:isolation & purification*; D006801:Humans; D010485:Periapical Periodontitis / Q000821:virology*; D060888:Real-Time Polymerase Chain Reaction; D055815:Young Adult