title: Expression and distribution of laminin receptor precursor/laminin receptor in rabbit tissues.
abstract: The 37/67-kDa laminin receptor precursor (LRP)/laminin receptor (LR) is a cell surface receptor for cellular prion proteins and misfolded pathological prions. Previous research has shown that blocking or decreasing LRP/LP levels by anti-LRP/LR antibodies or small interfering RNAs (siRNAs) can prolong the incubation phase of experimental prion infection. This study aimed to investigate potential mechanisms contributing to prion resistance/susceptibility by using the rabbit, a species unsusceptible to prion infection, as a model. We investigated the expression level and distribution of LRP/LR in rabbit tissues by real-time polymerase chain reaction and by immunochemical analysis with a monoclonal anti-67 kDa LR antibody. Our results showed LRP/LR mRNA expression in all the tissues examined. Very low LRP/LR expression levels were observed in central nervous system (CNS) tissues, whereas high expression levels were observed in reproductive and digestive tissues, which differed from the expression patterns that have been reported for prion-susceptible animals. The immunochemical staining results were generally consistent with the mRNA findings, although no LR protein was detected in CNS tissues. Our findings provide a basis for further studies on prion resistance in rabbits and other animal species.
authors.affiliation: State Key Laboratories for Agrobiotechnology, Key Lab of Animal Epidemiology and Zoonosis, Ministry of Agriculture, National Animal Transmissible Spongiform Encephalopathy Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.
authors.forename: Huinuan
authors.initials: H
authors.lastname: Wang
authors.forename: Lifeng
authors.initials: L
authors.lastname: Yang
authors.forename: Mohammed
authors.initials: M
authors.lastname: Kouadir
authors.forename: Rongrong
authors.initials: R
authors.lastname: Tan
authors.forename: Wenyu
authors.initials: W
authors.lastname: Wu
authors.forename: Huarong
authors.initials: H
authors.lastname: Zou
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.forename: Sher Hayat
authors.initials: SH
authors.lastname: Khan
authors.forename: Dongfeng
authors.initials: D
authors.lastname: Li
authors.forename: Xiangmei
authors.initials: X
authors.lastname: Zhou
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Yin
authors.forename: Yunsheng
authors.initials: Y
authors.lastname: Wang
authors.forename: Deming
authors.initials: D
authors.lastname: Zhao
mesh_terms: D000818:Animals; D002490:Central Nervous System / Q000378:metabolism; D004064:Digestive System / Q000378:metabolism; D009928:Organ Specificity; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011817:Rabbits; D017474:Receptors, Laminin / Q000235:genetics / Q000378:metabolism*; D014566:Urogenital System / Q000378:metabolism
chemical_list: D011498:Protein Precursors; D012333:RNA, Messenger; D017474:Receptors, Laminin