title: Recombinant supercharged polypeptides restore and improve biolubrication.
abstract: Recombinant supercharged polypeptides (SUPs) with low cytotoxicity are developed and applied to rejuvenate the lubrication of naturally occurring salivary conditioning films (SCFs). SUPs with 72 positive charges adsorbed and rigidified the SCFs and recruited mucins to form a hydrated layer. These SCFs with SUPs have higher mechanical strength and sustain lubricating effect for longer duration compared with only SCFs.
authors.affiliation: University of Groningen and University Medical Center Groningen, Department of Biomedical Engineering, W.J. Kolff Institute, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlands.
authors.forename: Deepak H
authors.initials: DH
authors.lastname: Veeregowda
authors.forename: Anke
authors.initials: A
authors.lastname: Kolbe
authors.forename: Henny C
authors.initials: HC
authors.lastname: van der Mei
authors.forename: Henk J
authors.initials: HJ
authors.lastname: Busscher
authors.forename: Andreas
authors.initials: A
authors.lastname: Herrmann
authors.forename: Prashant K
authors.initials: PK
authors.lastname: Sharma
mesh_terms: D000327:Adsorption; D006801:Humans; D008153:Lubrication; D010455:Peptides / Q000737:chemistry* / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000737:chemistry* / Q000494:pharmacology*; D012463:Saliva / Q000187:drug effects* / Q000378:metabolism
chemical_list: D010455:Peptides; D011994:Recombinant Proteins
keywords: biolubrication; protein therapeutics; scanning force microscopy; sjögren's syndrome; supercharged polypeptides