title: A cardiomyocyte-based biosensor for antiarrhythmic drug evaluation by simultaneously monitoring cell growth and beating.
abstract: Drug-induced cardiotoxicity greatly endangers the human health and results in resource waste. Also, it is a leading attribution to drug withdrawal and late-stage attrition in pharmaceutical industry. In the study, a dual function cardiomyocyte-based biosensor was introduced for rapid drug evaluation with xCELLigence RTCA Cardio system. The cardiomyocyte-based biosensor can monitor the cardiomyocyte growth and beating status simultaneously under the drug effects. Two typical cardiovascular drug, verapamil and flecainide were selected as treatment agents to test the performance of this biosensor. The experiment results showed that the performance of cardiomyocyte-based biosensor verified the basic drug effects by beating status and also tested the drug cytotoxicity by the cell index curves of cardiomyocyte growth. Based on the advanced sensor detection technology and cell culture technology, this cardiomyocyte-based biosensor will be a utility platform for the drug preclinical assessment.
authors.affiliation: Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou 310027, PR China.
authors.forename: Tianxing
authors.initials: T
authors.lastname: Wang
authors.forename: Ning
authors.initials: N
authors.lastname: Hu
authors.forename: Jiayue
authors.initials: J
authors.lastname: Cao
authors.forename: Jieying
authors.initials: J
authors.lastname: Wu
authors.forename: Kaiqi
authors.initials: K
authors.lastname: Su
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
mesh_terms: D000818:Animals; D000889:Anti-Arrhythmia Agents / Q000494:pharmacology*; D015374:Biosensing Techniques / Q000295:instrumentation*; D049109:Cell Proliferation; D002478:Cells, Cultured; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation*; D004867:Equipment Design; D005424:Flecainide / Q000494:pharmacology*; D006801:Humans; D032383:Myocytes, Cardiac / Q000166:cytology / Q000187:drug effects*; D051381:Rats; D014700:Verapamil / Q000494:pharmacology*
chemical_list: D000889:Anti-Arrhythmia Agents; D014700:Verapamil; D005424:Flecainide
keywords: Cardiotoxicity; Cell-based biosensor; Pharmacological assays; Primary neonatal rat cardiomyocyte; Real time cell analysis