title: Hollow fiber liquid-phase microextraction with in situ derivatization combined with gas chromatography-mass spectrometry for the determination of root exudate phenylamine compounds in hot pepper ( Capsicum annuum L.).
abstract: Hollow fiber liquid-phase microextraction (HF-LPME) with derivatization was developed for the determination of three root exudate phenylamine compounds in hot pepper ( Capsicum annuum L.) by gas chromatography-mass spectrometry (GC-MS). The performance and applicability of the proposed procedure were evaluated through the extraction of 1-naphthylamine (1-NA), diphenylamine (DPA), and N-phenyl-2- naphthaleneamine (N-P-2-NA) in a recirculating hydroponic solution of hot pepper. Parameters affecting the extraction efficiency were investigated. The calibration curves showed a good linearity in the range of 0.1-10 μg mL(-1). The limits of detection (S/N = 3) for the three compounds were 0.096, 0.074, and 0.057 μg mL(-1), respectively. The enrichment factors reached 174, 196, and 230 at the concentration of 5 μg mL(-1), and relative standard deviations (RSD) of 9.5, 8.6, and 7.8% and 8.4, 7.6, and 6.2% were obtained at concentrations of 2 and 5 μg mL(-1) for 1-NA, DPA, and N-P-2-NA, respectively. Recoveries ranging from 90.2 to 96.1% and RSDs below 9.1% were obtained when HF-LPME with in situ derivatization was applied to determine root exudate 1-NA, DPA, and N-P-2-NA after 15 and 30 days of culture solution, respectively.
authors.affiliation: Science Research Centre, Harbin Institute of Technology, Harbin 150001, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Sun
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000814:Aniline Compounds / Q000032:analysis* / Q000302:isolation & purification* / Q000378:metabolism; D002212:Capsicum / Q000737:chemistry* / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D059627:Liquid Phase Microextraction / Q000295:instrumentation / Q000379:methods*; D053147:Plant Exudates / Q000032:analysis* / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D000814:Aniline Compounds; D053147:Plant Exudates