title: The pathogenic A391E mutation in FGFR3 induces a structural change in the transmembrane domain dimer.
abstract: Fibroblast growth factor receptor 3 (FGFR3) is a single-pass membrane protein and a member of the receptor tyrosine kinase family of proteins that is involved in the regulation of skeletal growth and development. FGFR3 has three distinct domains: the ligand binding extracellular domain, the cytosolic kinase domain and the transmembrane domain (TMD). Previous work with the isolated FGFR3 TMD has shown that it has the ability to dimerize. Clinical and genetic studies have also correlated mutations in the TMD with a variety of skeletal and cranial dysplasias and cancer. Although the structures of the extracellular and cytosolic domains of FGFR3 have been solved, the structure of the TMD dimer is still unknown. Furthermore, very little is known regarding the effects of pathogenic mutations on the TMD dimer structure. We, therefore, carried out ToxR activity assays to determine the role of the SmXXXSm motif in the dimerization of the FGFR3 TMD. This motif has been shown to drive the association of many transmembrane proteins. Our results indicate that the interaction between wild-type FGFR3 TMDs is not mediated by two adjacent SmXXXSm motifs. In contrast, studies using the TMD carrying the pathogenic A391E mutation suggest that the motifs play a role in the dimerization of the mutant TMD. Based on these observations, here we report a new mechanistic model in which the pathogenic A391E mutation induces a structural change that leads to the formation of a more stable dimer.
authors.affiliation: Department of Biology, Saint Joseph's University, Philadelphia, PA 19107, USA.
authors.forename: Krishna C
authors.initials: KC
authors.lastname: Mudumbi
authors.forename: Ayse
authors.initials: A
authors.lastname: Julius
authors.forename: Jana
authors.initials: J
authors.lastname: Herrmann
authors.forename: Edwin
authors.initials: E
authors.lastname: Li
mesh_terms: D020816:Amino Acid Motifs; D019943:Amino Acid Substitution; D001848:Bone Diseases, Developmental / Q000235:genetics / Q000378:metabolism; D006801:Humans; D020125:Mutation, Missense; D009369:Neoplasms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D051498:Receptor, Fibroblast Growth Factor, Type 3 / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: C496365:FGFR3 protein, human; D051498:Receptor, Fibroblast Growth Factor, Type 3