title: Proteomics and transcriptomics of broccoli subjected to exogenously supplied and transgenic senescence-induced cytokinin for amelioration of postharvest yellowing.
abstract: Previously, we investigated transgenic broccoli harboring senescence-associated-gene (SAG) promoter-triggered isopentenyltransferase (ipt), which encodes the key enzyme for cytokinin (CK) synthesis and mimics the action of exogenous supplied CK in delaying postharvest senescence of broccoli. Here, we used proteomics and transcriptomics to compare the mechanisms of ipt-transgenic and N(6)-benzylaminopurine (BA) CK treatment of broccoli during postharvest storage. The 2 treatments conferred common and distinct mechanisms. BA treatment decreased the quantity of proteins involved in energy and carbohydrate metabolism and amino acid metabolism, and ipt-transgenic treatment increased that of stress-related proteins and molecular chaperones and slightly affected levels of carbohydrate metabolism proteins. Both treatments regulated genes involved in CK signaling, sugar transport, energy and carbohydrate metabolism, amino acid metabolism and lipid metabolism, although ipt-transgenic treatment to a lesser extent. BA treatment induced genes encoding molecular chaperones, whereas ipt-transgenic treatment induced stress-related genes for cellular protection during storage. Both BA and ipt-transgenic treatments acted antagonistically on ethylene functions. We propose a long-term acclimation of metabolism and protection systems with ipt-transgenic treatment of broccoli and short-term modulation of metabolism and establishment of a protection system with both BA and ipt-transgenic treatments in delaying senescence of broccoli florets.
  
  
  CONCLUSIONS
  Transgenic broccoli harboring senescence-associated-gene (SAG) promoter-triggered isopentenyltransferase (ipt), which encodes the key enzyme for cytokinin (CK) synthesis and N(6)-benzylaminopurine (BA) CK treated broccoli both showed retardation of postharvest senescence during storage. The mechanisms underlying the two treatments were compared. The combination of proteomic and transcriptomic evidences revealed that the 2 treatments conferred common and distinct mechanisms in delaying senescence of broccoli florets. We propose a long-term acclimation of metabolism and protection systems with ipt-transgenic treatment of broccoli and short-term modulation of metabolism and establishment of a protection system with both BA and ipt-transgenic treatments in delaying senescence of broccoli florets. This article is part of a Special Issue entitled: Translational Plant Proteomics.
authors.affiliation: Institute of Plant and Microbial Biology, Academia Sinica, Nankang, Taipei 11529, Taiwan.
authors.forename: Mao-Sen
authors.initials: MS
authors.lastname: Liu
authors.forename: Hui-Chun
authors.initials: HC
authors.lastname: Li
authors.forename: Ying-Mi
authors.initials: YM
authors.lastname: Lai
authors.forename: Hsiao-Feng
authors.initials: HF
authors.lastname: Lo
authors.forename: Long-Fang O
authors.initials: LF
authors.lastname: Chen
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000235:genetics*; D001593:Benzyl Compounds / Q000494:pharmacology; D001937:Brassica / Q000187:drug effects* / Q000235:genetics*; D003583:Cytokinins / Q000235:genetics / Q000378:metabolism; D035264:Flowers / Q000187:drug effects; D061353:Food Storage; D018506:Gene Expression Regulation, Plant; D010940:Plant Proteins / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000378:metabolism; D040901:Proteomics; D011687:Purines / Q000494:pharmacology; D059467:Transcriptome / Q000187:drug effects*
chemical_list: D001593:Benzyl Compounds; D003583:Cytokinins; D010940:Plant Proteins; D011687:Purines; D019883:Alkyl and Aryl Transferases; C024274:adenylate isopentenyltransferase; C480551:benzylaminopurine
keywords: Broccoli; Cytokinin; Isopentenyltransferase; Proteome; Senescence; Transcriptome