title: Involvement of 5-lipoxygenase in Alzheimer's disease: a role for DNA methylation.
abstract: Lipoxygenases play a major role in the neuropathology of Alzheimer's disease (AD), even though the underlying mechanisms are as yet poorly understood. Here, we studied the epigenetic regulation of 5-lipoxygenase (5-LOX) in peripheral blood mononuclear cells of subjects with late-onset AD and age-matched controls. We found a significant increase in 5-LOX gene expression in AD subjects compared to healthy controls, paralleled by increased 5-LOX protein and leukotriene B4, the 5-LOX product. In addition, a consistent reduction in DNA methylation at 5-LOX gene promoter was documented in AD versus healthy subjects. Taken together, our findings further support a role for 5-LOX in vulnerability to neurodegeneration.
authors.affiliation: Department of Biomedical Sciences, University of Teramo, Teramo, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Di Francesco
authors.forename: Beatrice
authors.initials: B
authors.lastname: Arosio
authors.forename: Cristina
authors.initials: C
authors.lastname: Gussago
authors.forename: Enrico
authors.initials: E
authors.lastname: Dainese
authors.forename: Daniela
authors.initials: D
authors.lastname: Mari
authors.forename: Claudio
authors.initials: C
authors.lastname: D'Addario
authors.forename: Mauro
authors.initials: M
authors.lastname: Maccarrone
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000175:diagnosis* / Q000201:enzymology*; D001094:Arachidonate 5-Lipoxygenase / Q000096:biosynthesis*; D019175:DNA Methylation / Q000502:physiology*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000201:enzymology*; D008297:Male
chemical_list: D001094:Arachidonate 5-Lipoxygenase