title: Geodermatophilus africanus sp. nov., a halotolerant actinomycete isolated from Saharan desert sand.
abstract: A novel Gram-strain positive, aerobic, actinobacterial strain, designated CF11/1(T), was isolated from a sand sample obtained in the Sahara Desert, Chad. The black-pigmented isolate was aerobic and exhibited optimal growth from 25 to 35 °C at pH 6.0-8.0 and with 0-8 % (w/v) NaCl, indicating that it is a halotolerant mesophile. Chemotaxonomic and molecular characteristics of the isolate matched those described for members of the genus Geodermatophilus. The G+C content in the genome was 74.4 mol%. The peptidoglycan contained meso-diaminopimelic acid as diagnostic diaminoacid. The main phospholipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol and a minor fraction of phosphatidylglycerol; MK-9(H4) was the dominant menaquinone, and galactose was detected as a diagnostic sugar. The major cellular fatty acid was branched-chain saturated acid iso-C16:0. Analysis of 16S rRNA gene sequences showed 95.3-98.6 % pairwise sequence identity with the members of the genus Geodermatophilus. Based on phenotypic and chemotaxonomic properties, as well as phylogenetic distinctiveness, the isolate represents a novel species, Geodermatophilus africanus, with the type strain CF11/1(T) (DSM 45422 = CCUG 62969 = MTCC 11556).
authors.affiliation: Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124, Braunschweig, Germany.
authors.forename: María del Carmen
authors.initials: Mdel C
authors.lastname: Montero-Calasanz
authors.forename: Markus
authors.initials: M
authors.lastname: Göker
authors.forename: Gabriele
authors.initials: G
authors.lastname: Pötter
authors.forename: Manfred
authors.initials: M
authors.lastname: Rohde
authors.forename: Cathrin
authors.initials: C
authors.lastname: Spröer
authors.forename: Peter
authors.initials: P
authors.lastname: Schumann
authors.forename: Anna A
authors.initials: AA
authors.lastname: Gorbushina
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Klenk
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002596:Chad; D004269:DNA, Bacterial / Q000235:genetics; D003889:Desert Climate; D005227:Fatty Acids / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012822:Silicon Dioxide / Q000032:analysis*; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012822:Silicon Dioxide