title: Comparative analysis of the genome sequences and replication profiles of chikungunya virus isolates within the East, Central and South African (ECSA) lineage.
abstract: BACKGROUND
  A comparative analysis of the genomic and replication profiles of different geographical chikungunya virus (CHIKV) isolates of the East, Central and South African (ECSA) lineage was performed.
  
  
  RESULTS
  Analysis of the data revealed the different growth kinetics for the different isolates. Deep genome sequencing analysis further revealed specific amino acid mutations in the viral nsP1, nsP3, nsP4, E1 and E2 proteins in the different isolates. Despite the difference in viral genomic profiles, the virus-induced ultrastructural changes within infected cells remained highly conserved among the different chikungunya virus isolates.
  
  
  CONCLUSIONS
  These findings provide insights into the genomic and replication profiles of the re-emerging chikungunya virus isolates of the ECSA lineage.
authors.affiliation: Laboratory of Molecular RNA Virology and Antiviral Strategies, Department of Microbiology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, MD4, 5 Science Drive 2, Singapore 117597, Singapore.
authors.forename: Karen Caiyun
authors.initials: KC
authors.lastname: Chen
authors.forename: Yiu-Wing
authors.initials: YW
authors.lastname: Kam
authors.forename: Raymond Tzer Pin
authors.initials: RT
authors.lastname: Lin
authors.forename: Mary Mah-Lee
authors.initials: MM
authors.lastname: Ng
authors.forename: Lisa Fp
authors.initials: LF
authors.lastname: Ng
authors.forename: Justin Jang Hann
authors.initials: JJ
authors.lastname: Chu
mesh_terms: D000349:Africa; D019943:Amino Acid Substitution; D000818:Animals; D002460:Cell Line; D002646:Chikungunya virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology*; D016679:Genome, Viral; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D020125:Mutation, Missense; D012367:RNA, Viral / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics; D014779:Virus Replication
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins