title: Comparison of five media for detection of extended-spectrum Beta-lactamase by use of the wasp instrument for automated specimen processing.
abstract: Overall, 2,337 rectal screening samples (RSSs) were seeded by using the Wasp instrument for automated microbiological processing with five media for detection of extended-spectrum β-lactamase (ESBL): CHROMagar, ChromID, Brilliance, BD Drigalski, and HEGP media. Of 354 RSSs harboring ESBL-producing isolates, 89.3% were found to be positive on all media. Sensitivity and specificity ranged from 95.5 to 98.3% and from 57.9 to 72.3%, respectively. No medium was perfectly ESBL selective, and non-ESBL-producing strains were mainly Enterobacteriaceae overproducing AmpC β-lactamase and nonfermenting Gram-negative bacilli, mostly Pseudomonas aeruginosa.
authors.affiliation: Service de Microbiologie, Assistance Publique-Hôpitaux de Paris, Hôpital Européen Georges-Pompidou, Paris, France. patrick.grohs@egp.aphp.fr
authors.forename: P
authors.initials: P
authors.lastname: Grohs
authors.forename: B
authors.initials: B
authors.lastname: Tillecovidin
authors.forename: A
authors.initials: A
authors.lastname: Caumont-Prim
authors.forename: E
authors.initials: E
authors.lastname: Carbonnelle
authors.forename: N
authors.initials: N
authors.lastname: Day
authors.forename: I
authors.initials: I
authors.lastname: Podglajen
authors.forename: L
authors.initials: L
authors.lastname: Gutmann
mesh_terms: D057205:Automation, Laboratory / Q000379:methods*; D001431:Bacteriological Techniques / Q000379:methods*; D003470:Culture Media / Q000737:chemistry*; D005243:Feces / Q000382:microbiology; D006090:Gram-Negative Bacteria / Q000201:enzymology* / Q000302:isolation & purification; D006801:Humans; D012680:Sensitivity and Specificity; D001618:beta-Lactamases / Q000032:analysis*
chemical_list: D003470:Culture Media; D001618:beta-Lactamases