title: Characterization of a monoclonal antibody that specifically inhibits triosephosphate isomerase activity of Taenia solium.
abstract: In the present study, we obtained and characterized partially a monoclonal antibody (4H11D10B11 mAb) against triosephosphate isomerase from Taenia solium (TTPI). This antibody recognized the enzyme by both ELISA and western blot and was able to inhibit its enzymatic activity in 74%. Moreover, the antigen-binding fragments (Fabs), products of digestion of the monoclonal antibody with papain, retained almost the same inhibitory effect. We determined the binding site by ELISA; synthetic peptides containing sequences from different non-conserved regions of the TTPI were confronted to the 4H11D10B11 mAb. The epitope recognized by the monoclonal antibody was located on peptide TTPI-56 (ATPAQAQEVHKVVRDWIRKHVDAGIADKARI), and an analysis of mimotopes, obtained with the 4H11D10B11 mAb, suggests that the epitope spans the sequence WIRKHVDAGIAD, residues 193-204 of the enzyme. This epitope is located within helix 6, next to loop 6, an essential active loop during catalysis. The antibody did not recognize triosephosphate isomerase from man and pig, definitive and intermediary hosts of T. solium, respectively. Furthermore, it did not bind to the catalytic site, since kinetic analysis demonstrated that inhibition had a non-competitive profile.
authors.affiliation: Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de Mexico, Edificio A, 2(o) Piso, Ciudad Universitaria, Mexico DF 04510, Mexico.
authors.forename: Sanabria-Ayala
authors.initials: SA
authors.lastname: Víctor
authors.forename: Medina-Flores
authors.initials: MF
authors.lastname: Yolanda
authors.forename: Zavala-Carballo
authors.initials: ZC
authors.lastname: Araceli
authors.forename: Jiménez
authors.initials: J
authors.lastname: Lucía
authors.forename: Landa
authors.initials: L
authors.lastname: Abraham
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology* / Q000494:pharmacology; D000939:Epitopes / Q000737:chemistry; D006825:Hybridomas; D007140:Immunoglobulin Fab Fragments / Q000032:analysis / Q000737:chemistry / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D041201:Taenia solium / Q000201:enzymology* / Q000276:immunology; D014305:Triose-Phosphate Isomerase / Q000037:antagonists & inhibitors / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000939:Epitopes; D007140:Immunoglobulin Fab Fragments; D014305:Triose-Phosphate Isomerase
keywords: Epitope; Mimotope; Monoclonal antibody; Phage display; Taenia solium; Triosephosphate isomerase