title: Biodegradation of low concentrations of 1,2-dibromoethane in groundwater is enhanced by phenol.
abstract: The lead scavenger 1,2-dibromoethane (EDB), a former additive to leaded gasoline, is a common groundwater contaminant, yet not much knowledge is available for its targeted bioremediation, especially under in situ conditions. The study site was an aviation gas spill site, which, although all hydrocarbons and most of the EDB were remediated in the mid-1990s, still exhibits low levels of EDB remaining in the groundwater (about 11 μg EDB/l). To evaluate the effect of phenol on biostimulation of low concentration of EDB, microcosms were established from an EDB-contaminated aquifer. After 300 days at environmentally relevant conditions (12 ± 2 °C, static incubation), EDB was not significantly removed from unamended microcosms compared to the abiotic control. However, in treatments amended with phenol, up to 80 % of the initial EDB concentration had been degraded, while added phenol was removed completely. Microbial community composition in unamended and phenol-amended microcosms remained unchanged, and Polaromonas sp. dominated both types of microcosms, but total bacterial abundance and numbers of the gene for phenol hydroxylase were higher in phenol-amended microcosms. Dehalogenase, an indicator suggesting targeted aerobic biodegradation of EDB, was not detected in either treatment. This finding suggests phenol hydroxylase, rather than a dehalogenation reaction, may be responsible for 1,2-dibromoethane oxidation under in situ conditions. In addition, biostimulation of EDB is possible through the addition of low levels of phenol in aerobic groundwater sites.
authors.affiliation: Department of Microbiology, University of Massachusetts, Amherst, MA, 01003, USA.
authors.forename: Kyunghwa
authors.initials: K
authors.lastname: Baek
authors.forename: Meng
authors.initials: M
authors.lastname: Wang
authors.forename: Robert
authors.initials: R
authors.lastname: McKeever
authors.forename: Kahlil
authors.initials: K
authors.lastname: Rieber
authors.forename: Chul
authors.initials: C
authors.lastname: Park
authors.forename: Klaus
authors.initials: K
authors.lastname: Nüsslein
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D058448:Biota; D015946:Ethylene Dibromide / Q000378:metabolism*; D060587:Groundwater / Q000737:chemistry*; D053858:Metabolic Networks and Pathways / Q000235:genetics; D019800:Phenol / Q000378:metabolism*; D014873:Water Pollutants / Q000378:metabolism*
chemical_list: D014873:Water Pollutants; D015946:Ethylene Dibromide; D019800:Phenol