title: The expression and proangiogenic effect of nucleolin during the recovery of heat-denatured HUVECs.
abstract: BACKGROUND
  The present study aims to examine the expression patterns and roles of nucleolin during the recovery of heat-denatured human umbilical vein endothelial cells (HUVECs).
  
  
  METHODS
  Deep partial thickness burn model in Sprague-Dawley rats and the heat denatured cell model (52°C, 35s) were used. The expression of nucleolin was measured using Western blot analysis and real-time PCR. Angiogenesis was assessed using in vitro parameters including endothelial cell proliferation, transwell migration assay, and scratched wound healing. Gene transfection and RNA interference approaches were employed to investigate the roles of nucleolin.
  
  
  RESULTS
  Nucleolin mRNA and protein expression showed a time-dependent increase during the recovery of heat-denatured dermis and HUVECs. Heat-denaturation time-dependently promoted cell growth, adhesion, migration, scratched wound healing and formation of tube-like structures in HUVECs. These effects of heat denaturation on endothelial wound healing and formation of tube-like structures were prevented by knockdown of nucleolin, whereas over-expression of nucleolin increased cell growth, migration, and formation of tube-like structures in cultured HUVEC endothelial cells. In addition, we found that the expression of vascular endothelial growth factor (VEGF) increased during the recovery of heat-denatured dermis and HUVECs, and nucleolin up-regulated VEGF in HUVECs.
  
  
  CONCLUSIONS
  The present study reveals that the expression of nucleolin is up-regulated, and plays a pro-angiogenic role during the recovery of heat-denatured dermis and its mechanism is probably dependent on production of VEGF.
  
  
  CONCLUSIONS
  We find a novel and important pro-angiogenic role of nucleolin during the recovery of heat-denatured dermis.
authors.affiliation: Department of Burns and Plastic Surgery, Central South University, Changsha, Hunan, People's Republic of China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Liang
authors.forename: Bimei
authors.initials: B
authors.lastname: Jiang
authors.forename: Chunliu
authors.initials: C
authors.lastname: Lv
authors.forename: Xu
authors.initials: X
authors.lastname: Huang
authors.forename: Li
authors.initials: L
authors.lastname: Sun
authors.forename: Pihong
authors.initials: P
authors.lastname: Zhang
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Huang
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D006358:Hot Temperature; D006801:Humans; D018919:Neovascularization, Physiologic / Q000235:genetics; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D000097796:Nucleolin
chemical_list: D010750:Phosphoproteins; D016601:RNA-Binding Proteins; D042461:Vascular Endothelial Growth Factor A; D000097796:Nucleolin
keywords: Angiogenesis; Heat-denatured; Human umbilical vein endothelial cells; Nucleolin; Vascular endothelial growth factor