title: Expression, purification and preliminary crystallographic analysis of the T6SS effector protein Tse3 from Pseudomonas aeruginosa.
abstract: Pseudomonas aeruginosa uses the type VI secretion system (T6SS) to inject effector proteins into rival cells in niche competition. Tse3, one of the effectors of T6SS, is delivered into the periplasm of recipient cells. Tse3 functions as a muramidase that degrades the β-1,4-linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) in peptidoglycan, thus leading to lysis of the recipient cells and providing a competitive advantage to the donor cells. Here, the preliminary crystallographic study of Tse3 is reported. A crystal of Tse3 diffracted to 1.5 Å resolution. It belonged to space group C121, with unit-cell parameters a = 166.99, b = 70.13, c = 41.94 Å, α = 90.00, β = 90.52, γ  = 90.00° and one molecule per asymmetric unit.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.
authors.forename: Defen
authors.initials: D
authors.lastname: Lu
authors.forename: Guijun
authors.initials: G
authors.lastname: Shang
authors.forename: Qian
authors.initials: Q
authors.lastname: Yu
authors.forename: Heqiao
authors.initials: H
authors.lastname: Zhang
authors.forename: Yanyu
authors.initials: Y
authors.lastname: Zhao
authors.forename: Huaixing
authors.initials: H
authors.lastname: Cang
authors.forename: Lichuan
authors.initials: L
authors.lastname: Gu
authors.forename: Sujuan
authors.initials: S
authors.lastname: Xu
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000737:chemistry* / Q000235:genetics; D018360:Crystallography, X-Ray; D015964:Gene Expression Regulation, Bacterial; D015971:Gene Expression Regulation, Enzymologic; D008969:Molecular Sequence Data; D011550:Pseudomonas aeruginosa
chemical_list: D001426:Bacterial Proteins
keywords: Pseudomonas aeruginosa; Tse3; muramidases; type VI secretion system