title: Antiproliferative Effect of Solanum nigrum on Human Leukemic Cell Lines.
abstract: Solanum nigrum is used in various traditional medical systems for antiproliferative, antiinflammatory, antiseizure and hepatoprotective activities. We have evaluated organic solvent and aqueous extracts obtained from berries of Solanum nigrum for antiproliferative activity on leukemic cell lines, Jurkat and HL-60 (Human promyelocytic leukemia cells). The cell viability after the treatment with Solanum nigrum extract was measured by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay. Results indicated increased cytotoxicity with increasing extract concentrations. Comparative analysis indicated that 50% inhibitory concentration value of methanol extract is the lowest on both cell lines.
authors.affiliation: Department of Biotechnology, Jaypee Institute of Information Technology, Noida-201 307, India.
authors.forename: Reema
authors.initials: R
authors.lastname: Gabrani
authors.forename: Ramya
authors.initials: R
authors.lastname: Jain
authors.forename: Anjali
authors.initials: A
authors.lastname: Sharma
authors.forename: Indira P
authors.initials: IP
authors.lastname: Sarethy
authors.forename: Shweta
authors.initials: S
authors.lastname: Dang
authors.forename: S
authors.initials: S
authors.lastname: Gupta
keywords: Cytotoxicity; HL-60; Jurkat; MTT assay; Solanum nigrum