title: Enhanced therapeutic effect of cisplatin on the prostate cancer in tumor-bearing mice by transfecting the attenuated Salmonella carrying a plasmid co-expressing p53 gene and mdm2 siRNA.
abstract: Prostate cancer urgently needs an efficient therapy. Here we demonstrated that cisplatin combined with gene therapy by transfecting the attenuated Salmonella that carry a plasmid containing p53 gene and MDM2 siRNA provided a super-synergistic effect on the inhibition of prostate cancer growth in vivo. This synergistic therapy was associated with the induction of apoptotic cell death with a decreased Bcl2 to Bax expression ratio and increased expression of cleaved caspase 3 and caspase 9 in the prostate cancer xenograft. These results indicate that cisplatin-chemotherapy in combination with targeting the MDM2/p53 axis is an attractive strategy to treat prostate cancer.
authors.affiliation: Cancer Center at the First Hospital of Jilin University, Changchun 130021, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.forename: Changdong
authors.initials: C
authors.lastname: Zhou
authors.forename: Junlian
authors.initials: J
authors.lastname: Gu
authors.forename: Yanan
authors.initials: Y
authors.lastname: Liu
authors.forename: Lijing
authors.initials: L
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Guanjun
authors.initials: G
authors.lastname: Wang
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.forename: Lu
authors.initials: L
authors.lastname: Cai
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000627:therapeutic use*; D017209:Apoptosis / Q000187:drug effects; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000627:therapeutic use*; D003131:Combined Modality Therapy; D016158:Genes, p53; D015316:Genetic Therapy; D006801:Humans; D008297:Male; D051379:Mice; D011471:Prostatic Neoplasms / Q000628:therapy*; D051736:Proto-Oncogene Proteins c-mdm2 / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics*; D012486:Salmonella typhimurium / Q000235:genetics
chemical_list: D000970:Antineoplastic Agents; D034741:RNA, Small Interfering; C497206:MDM2 protein, human; D051736:Proto-Oncogene Proteins c-mdm2; D002945:Cisplatin
keywords: Apoptosis; Cisplatin (DDP); P53; Prostate cancer; Si RNA-mdm2; Xenograft mice model