title: [Performance effect of rabbit carotid artery treated with decellularization and photo-oxidation].
abstract: OBJECTIVE
  To analyze and discuss the feasibility of rabbit carotid artery treated with decellularization and photo-oxidation.
  
  
  METHODS
  Sixty vascular slices of rabbit carotid artery were divided into a fresh group, a cryopreservation group, a glutaraldehyde group, and a decellularization plus photo-oxidation group 15 in each group. To evaluate the physical properties of all the rabbit carotid arteries by testing heat-shrinking temperature, tensile stress and the max elongation of each group. Then by buliding subcutaneous embedding model in SD rats we evaluated the biological stability and the anti-calcification function property of the above rabbit carotid arteries, and the detection means included HE stain, atomic absorption spectrometry and Von-Kossa calcium salt stain.
  
  
  RESULTS
  The heat-shrinking temperature, tensile stress and the max elongation in the cryopreservation group were lower or shorter than those of the other groups and the difference had statistical significance (P<0.05). Although the heat-shrinking temperature and the tensile stress in the decellularization plus photo-oxidation group were lower or shorter than those in the glutaraldehyde group (P<0.05), the max elongation in the decellularization plus photo-oxidation group was much longer than that in the glutaraldehyde group (P<0.05). The rabbit carotid artery treated with decellularization plus photo-oxidation showed lower immunogenicity and better biological stability and better anti-calcification property compared with the other groups.
  
  
  CONCLUSIONS
  Decellularization associated with photo-oxidation is a suitable and novel protocol for small caliber artery allograft with a diameter of less than 6 mm which is unbreakable to mechanical properties and conducive to biological stability, which has a broad prospect.
authors.affiliation: Department of Cardiac Surgery, First Affiliated Hospital of Xiamen University, Xiamen Fujian 361001,China.
authors.forename: Feng
authors.initials: F
authors.lastname: Kuang
authors.forename: Zhonggui
authors.initials: Z
authors.lastname: Shan
authors.forename: Wenjun
authors.initials: W
authors.lastname: You
authors.forename: Xinmin
authors.initials: X
authors.lastname: Zhou
authors.forename: Bangliang
authors.initials: B
authors.lastname: Yin
authors.forename: Xiaobiao
authors.initials: X
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001807:Blood Vessel Prosthesis; D002114:Calcinosis / Q000517:prevention & control*; D002339:Carotid Arteries / Q000166:cytology* / Q000637:transplantation; D002469:Cell Separation / Q000379:methods*; D005260:Female; D016591:Histocytological Preparation Techniques; D008297:Male; D010083:Oxidants, Photochemical / Q000494:pharmacology*; D010084:Oxidation-Reduction; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D014183:Transplantation, Heterologous
chemical_list: D010083:Oxidants, Photochemical