title: Digestion kinetics of potato protein isolates in vitro and in vivo.
abstract: Recently, an industrial process was developed to isolate native protein fractions from potato: a high (HMW) and a low (LMW) molecular weight fraction. Digestion kinetics of HMW and LMW was studied in vitro and in vivo and compared with reference proteins. Under simulated conditions, highest digestion was found for whey protein, followed by soy, pea, HMW, casein and LMW. Ingestion of 20 g of proteins by eight healthy subjects (following a randomized, double-blind, cross-over design) induced a slow and moderate increase with HMW and LMW, while a peaked and high increase with whey protein, in postprandial plasma amino acid levels. Casein gave a similar profile as HMW, with higher levels. Contrary to whey and casein, HMW and LMW did not result in any changes in plasma insulin or glucose levels. This study provides insights in digestion of native potato protein isolates to assist their use as protein sources in food applications.
authors.affiliation: AVEBE U.A. , Veendam , the Netherlands and.
authors.forename: Tao
authors.initials: T
authors.lastname: He
authors.forename: Robin E J
authors.initials: RE
authors.lastname: Spelbrink
authors.forename: Ben J
authors.initials: BJ
authors.lastname: Witteman
authors.forename: Marco L F
authors.initials: ML
authors.lastname: Giuseppin
mesh_terms: D000293:Adolescent; D000328:Adult; D000596:Amino Acids / Q000097:blood*; D001786:Blood Glucose / Q000378:metabolism; D002364:Caseins / Q000378:metabolism; D018592:Cross-Over Studies; D004032:Diet; D004044:Dietary Proteins / Q000378:metabolism* / Q000494:pharmacology; D004063:Digestion; D004311:Double-Blind Method; D005260:Female; D006801:Humans; D007328:Insulin / Q000097:blood; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D008894:Milk Proteins / Q000378:metabolism / Q000494:pharmacology; D008970:Molecular Weight; D018532:Pisum sativum / Q000737:chemistry; D010940:Plant Proteins / Q000378:metabolism* / Q000494:pharmacology; D035281:Plant Tubers / Q000737:chemistry*; D011198:Solanum tuberosum / Q000737:chemistry*; D030262:Soybean Proteins / Q000378:metabolism / Q000494:pharmacology; D013025:Glycine max / Q000737:chemistry; D000067816:Whey Proteins; D055815:Young Adult
chemical_list: D000596:Amino Acids; D001786:Blood Glucose; D002364:Caseins; D004044:Dietary Proteins; D007328:Insulin; D008894:Milk Proteins; D010940:Plant Proteins; D030262:Soybean Proteins; D000067816:Whey Proteins