title: Aurora B spatially regulates EB3 phosphorylation to coordinate daughter cell adhesion with cytokinesis.
abstract: During mitosis, human cells round up, decreasing their adhesion to extracellular substrates. This must be quickly reestablished by poorly understood cytoskeleton remodeling mechanisms that prevent detachment from epithelia, while ensuring the successful completion of cytokinesis. Here we show that the microtubule end-binding (EB) proteins EB1 and EB3 play temporally distinct roles throughout cell division. Whereas EB1 was involved in spindle orientation before anaphase, EB3 was required for stabilization of focal adhesions and coordinated daughter cell spreading during mitotic exit. Additionally, EB3 promoted midbody microtubule stability and, consequently, midbody stabilization necessary for efficient cytokinesis. Importantly, daughter cell adhesion and cytokinesis completion were spatially regulated by distinct states of EB3 phosphorylation on serine 176 by Aurora B. This EB3 phosphorylation was enriched at the midbody and shown to control cortical microtubule growth. These findings uncover differential roles of EB proteins and explain the importance of an Aurora B phosphorylation gradient for the spatiotemporal regulation of microtubule function during mitotic exit and cytokinesis.
authors.affiliation: Chromosome Instability and Dynamics Laboratory, Instituto de Biologia Molecular e Celular (IBMC), University of Porto, Porto, Portugal.
authors.forename: Jorge G
authors.initials: JG
authors.lastname: Ferreira
authors.forename: António J
authors.initials: AJ
authors.lastname: Pereira
authors.forename: Anna
authors.initials: A
authors.lastname: Akhmanova
authors.forename: Helder
authors.initials: H
authors.lastname: Maiato
mesh_terms: D064107:Aurora Kinase B; D064127:Aurora Kinases; D002448:Cell Adhesion; D048749:Cytokinesis; D006367:HeLa Cells; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000378:metabolism / Q000502:physiology*; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism / Q000502:physiology*; D008941:Spindle Apparatus / Q000378:metabolism
chemical_list: C500985:MAPRE1 protein, human; C403664:MAPRE3 protein, human; D008869:Microtubule-Associated Proteins; C576921:AURKB protein, human; D064107:Aurora Kinase B; D064127:Aurora Kinases; D017346:Protein Serine-Threonine Kinases