title: Separation and characterization of sucrose esters from Oriental tobacco leaves using accelerated solvent extraction followed by SPE coupled to HPLC with ion-trap MS detection.
abstract: Sucrose esters (SEs) were successfully extracted from Oriental tobacco leaves using a new methodology based on accelerated solvent extraction followed by hydrophilic-lipophilic balanced cartridge cleanup step. The SEs were detected by HPLC with ion-trap MS detection using an electrospray interface operated in the positive ion mode. This methodology combines the high efficiency of extraction provided by a pressurized fluid and the highly sensitive characterization offered by ion-trap MS. Under the optimized conditions, 14 SEs were first identified among a total of 23 SEs found in Oriental tobacco leaves. Under the same conditions, only four new SEs were extracted by using traditional ultrasound-assisted extraction and liquid-solid extraction methods. The present method might be potentially useful in high-efficiency extraction and sensitive characterization of SEs from complex matrices such as tobacco leaves.
authors.affiliation: Department of Chemistry, University of Science and Technology of China, Anhui, PR China.
authors.forename: Hongying
authors.initials: H
authors.lastname: Zhu
authors.forename: Yonggang
authors.initials: Y
authors.lastname: Feng
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Wenjie
authors.initials: W
authors.lastname: Pan
authors.forename: Zhanghai
authors.initials: Z
authors.lastname: Li
authors.forename: Yonggao
authors.initials: Y
authors.lastname: Tu
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Zhu
authors.forename: Guangming
authors.initials: G
authors.lastname: Huang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation; D004952:Esters / Q000302:isolation & purification*; D013058:Mass Spectrometry / Q000295:instrumentation; D018515:Plant Leaves / Q000737:chemistry*; D052616:Solid Phase Extraction / Q000295:instrumentation; D012997:Solvents / Q000737:chemistry; D013395:Sucrose / Q000302:isolation & purification*; D014026:Nicotiana / Q000737:chemistry*
chemical_list: D004952:Esters; D012997:Solvents; D013395:Sucrose
keywords: Accelerated solvent extraction; LC-MS; SPE; Sucrose esters; Tobacco