title: Assessment of spectral Doppler in preclinical ultrasound using a small-size rotating phantom.
abstract: Preclinical ultrasound scanners are used to measure blood flow in small animals, but the potential errors in blood velocity measurements have not been quantified. This investigation rectifies this omission through the design and use of phantoms and evaluation of measurement errors for a preclinical ultrasound system (Vevo 770, Visualsonics, Toronto, ON, Canada). A ray model of geometric spectral broadening was used to predict velocity errors. A small-scale rotating phantom, made from tissue-mimicking material, was developed. True and Doppler-measured maximum velocities of the moving targets were compared over a range of angles from 10° to 80°. Results indicate that the maximum velocity was overestimated by up to 158% by spectral Doppler. There was good agreement (<10%) between theoretical velocity errors and measured errors for beam-target angles of 50°-80°. However, for angles of 10°-40°, the agreement was not as good (>50%). The phantom is capable of validating the performance of blood velocity measurement in preclinical ultrasound.
authors.affiliation: British Heart Foundation Centre for Cardiovascular Science, University of Edinburgh, Edinburgh, UK. ukxinyang@gmail.com
authors.forename: Xin
authors.initials: X
authors.lastname: Yang
authors.forename: Chao
authors.initials: C
authors.lastname: Sun
authors.forename: Tom
authors.initials: T
authors.lastname: Anderson
authors.forename: Carmel M
authors.initials: CM
authors.lastname: Moran
authors.forename: Patrick W F
authors.initials: PW
authors.lastname: Hadoke
authors.forename: Gillian A
authors.initials: GA
authors.lastname: Gray
authors.forename: Peter R
authors.initials: PR
authors.lastname: Hoskins
mesh_terms: D000818:Animals; D017076:Computer-Aided Design; D004353:Drug Evaluation, Preclinical / Q000295:instrumentation* / Q000662:veterinary*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D051379:Mice; D008904:Miniaturization; D019047:Phantoms, Imaging / Q000662:veterinary*; D051381:Rats; D015203:Reproducibility of Results; D012399:Rotation; D012680:Sensitivity and Specificity; D018608:Ultrasonography, Doppler / Q000295:instrumentation* / Q000662:veterinary*
keywords: Blood velocity; Doppler phantom; Doppler ultrasound; High-frequency ultrasound; Preclinical ultrasound