title: De Novo assembly and comparative analysis of the Enterococcus faecalis genome (KACC 91532) from a Korean Neonate.
abstract: Using a newly constructed de novo assembly pipeline, finished genome level assembly had been conducted for the probiotic candidate strain E. faecalis KACC 91532 isolated from a stool samples of Korean neonates. Our gene prediction identified 3,061 genes in the assembled genome of the strain. Among these, nine genes were specific only for the E. faecalis KACC 91532, compared with all of the four known reference genomes (EF62, D32, V583, OG1RF). We identified genes related to phenotypic characters and detected E. faecalis KACC 91532-specific evolutionarily accelerated genes using dN/dS analysis. From these results, we found the potential risk of KACC 91532 as a useful probiotic strain and identified some candidate genetic variations that could affect the function of enzymes.
authors.affiliation: Animal Products Research and Development Division, National Institute of Animal Science, Rural Development Administration, Suwon, 441-706, Republic of Korea.
authors.forename: Jun Sang
authors.initials: JS
authors.lastname: Ham
authors.forename: Woori
authors.initials: W
authors.lastname: Kwak
authors.forename: Oun Ki
authors.initials: OK
authors.lastname: Chang
authors.forename: Gi Sung
authors.initials: GS
authors.lastname: Han
authors.forename: Seok Geun
authors.initials: SG
authors.lastname: Jeong
authors.forename: Kuk Hwan
authors.initials: KH
authors.lastname: Seol
authors.forename: Hyoun Wook
authors.initials: HW
authors.lastname: Kim
authors.forename: Geun Ho
authors.initials: GH
authors.lastname: Kang
authors.forename: Beom Young
authors.initials: BY
authors.lastname: Park
authors.forename: Hyun-Jeong
authors.initials: HJ
authors.lastname: Lee
authors.forename: Jong Geun
authors.initials: JG
authors.lastname: Kim
authors.forename: Kyu-Won
authors.initials: KW
authors.lastname: Kim
authors.forename: Samsun
authors.initials: S
authors.lastname: Sung
authors.forename: Taeheon
authors.initials: T
authors.lastname: Lee
authors.forename: Seoae
authors.initials: S
authors.lastname: Cho
authors.forename: Heebal
authors.initials: H
authors.lastname: Kim
mesh_terms: D044466:Asian People; D004269:DNA, Bacterial / Q000737:chemistry* / Q000235:genetics*; D013293:Enterococcus faecalis / Q000235:genetics* / Q000302:isolation & purification; D005243:Feces / Q000382:microbiology; D016680:Genome, Bacterial; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology; D006801:Humans; D007231:Infant, Newborn; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial