title: Development and characterization of West Nile virus replicon expressing secreted Gaussia luciferase.
abstract: We developed a Gaussia luciferase (Gluc) reporter replicon of West Nile virus (WNV) and used it to quantify viral translation and RNA replication. The advantage of the Gluc replicon is that Gaussia luciferase is secreted into the culture medium from cells transfected with Gluc replicon RNA, and the medium can be assayed directly for luciferase activity. Using a known Flavivirus inhibitor (NITD008), we demonstrated that the Gluc-WNV replicon could be used for antiviral screening. The Gluc-WNV-Rep will be useful for research in antiviral drug development programs, as well as for studying viral replication and pathogenesis of WNV.
authors.affiliation: Center for Emerging Infectious Diseases, State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Shan
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Li
authors.forename: Chenglin
authors.initials: C
authors.lastname: Deng
authors.forename: Baodi
authors.initials: B
authors.lastname: Shang
authors.forename: Linlin
authors.initials: L
authors.lastname: Xu
authors.forename: Hanqing
authors.initials: H
authors.lastname: Ye
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Yuan
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D003470:Culture Media / Q000737:chemistry; D004353:Drug Evaluation, Preclinical / Q000379:methods; D017930:Genes, Reporter; D008156:Luciferases / Q000235:genetics / Q000378:metabolism*; D013194:Staining and Labeling / Q000379:methods*; D014902:West Nile virus / Q000201:enzymology* / Q000235:genetics
chemical_list: D003470:Culture Media; D008156:Luciferases