title: Molecular imaging of hepatocellular carcinoma xenografts with epidermal growth factor receptor targeted affibody probes.
abstract: Hepatocellular carcinoma (HCC) is a highly aggressive and lethal cancer. It is typically asymptomatic at the early stage, with only 10%-20% of HCC patients being diagnosed early enough for appropriate surgical treatment. The delayed diagnosis of HCC is associated with limited treatment options and much lower survival rates. Therefore, the early and accurate detection of HCC is crucial to improve its currently dismal prognosis. The epidermal growth factor receptor (EGFR) has been reported to be involved in HCC tumorigenesis and to represent an attractive target for HCC imaging and therapy. In this study, an affibody molecule, Ac-Cys-ZEGFR:1907, targeting the extracellular domain of EGFR, was used for the first time to assess its potential to detect HCC xenografts. By evaluating radio- or fluorescent-labeled Ac-Cys-ZEGFR:1907 as a probe for positron emission tomography (PET) or optical imaging of HCC, subcutaneous EGFR-positive HCC xenografts were found to be successfully imaged by the PET probe. Thus, affibody-based PET imaging of EGFR provides a promising approach for detecting HCC in vivo.
authors.affiliation: Department of Digestive, China-Japan Union Hospital, Jilin University, Changchun, Jilin 130033, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhao
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Yang
authors.forename: Shibo
authors.initials: S
authors.lastname: Qi
authors.forename: Hongguang
authors.initials: H
authors.lastname: Liu
authors.forename: Han
authors.initials: H
authors.lastname: Jiang
authors.forename: Susan
authors.initials: S
authors.lastname: Hoppmann
authors.forename: Qizhen
authors.initials: Q
authors.lastname: Cao
authors.forename: Mei-Sze
authors.initials: MS
authors.lastname: Chua
authors.forename: Samuel K
authors.initials: SK
authors.lastname: So
authors.forename: Zhen
authors.initials: Z
authors.lastname: Cheng
mesh_terms: D000818:Animals; D063646:Carcinogenesis / Q000235:genetics / Q000473:pathology; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D055088:Early Detection of Cancer; D066246:ErbB Receptors / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D008113:Liver Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000473:pathology*; D051379:Mice; D057054:Molecular Imaging; D009367:Neoplasm Staging; D017434:Protein Structure, Tertiary; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000276:immunology; D023041:Xenograft Model Antitumor Assays
chemical_list: C569382:(Z(EGFR)-1907)2 affibody; D011993:Recombinant Fusion Proteins; D066246:ErbB Receptors