title: Structure of the Yersinia pestis tip protein LcrV refined to 1.65 Å resolution.
abstract: The human pathogen Yersinia pestis requires the assembly of the type III secretion system (T3SS) for virulence. The structural component of the T3SS contains an external needle and a tip complex, which is formed by LcrV in Y. pestis. The structure of an LcrV triple mutant (K40A/D41A/K42A) in a C273S background has previously been reported to 2.2 Å resolution. Here, the crystal structure of LcrV without the triple mutation in a C273S background is reported at a higher resolution of 1.65 Å. Overall the two structures are similar, but there are also notable differences, particularly near the site of the triple mutation. The refined structure revealed a slight shift in the backbone positions of residues Gly28-Asn43 and displayed electron density in the loop region consisting of residues Ile46-Val63, which was disordered in the original structure. In addition, the helical turn region spanning residues Tyr77-Gln95 adopts a different orientation.
authors.affiliation: Department of Molecular Biosciences, University of Kansas, Lawrence, KS 66045, USA.
authors.forename: Sukanya
authors.initials: S
authors.lastname: Chaudhury
authors.forename: Kevin P
authors.initials: KP
authors.lastname: Battaile
authors.forename: Scott
authors.initials: S
authors.lastname: Lovell
authors.forename: Gregory V
authors.initials: GV
authors.lastname: Plano
authors.forename: Roberto N
authors.initials: RN
authors.lastname: De Guzman
mesh_terms: D000942:Antigens, Bacterial / Q000737:chemistry* / Q000235:genetics; D018360:Crystallography, X-Ray; D009154:Mutation; D052899:Pore Forming Cytotoxic Proteins / Q000737:chemistry* / Q000235:genetics; D017433:Protein Structure, Secondary; D015010:Yersinia pestis / Q000235:genetics
chemical_list: D000942:Antigens, Bacterial; C043908:LcrV protein, Yersinia; D052899:Pore Forming Cytotoxic Proteins
keywords: LcrV; Yersinia pestis; tip proteins; type III secretion system