title: Angelica sinensis polysaccharide nanoparticles as novel non-viral carriers for gene delivery to mesenchymal stem cells.
abstract: This study centers on the use of a nanoparticle based on the polysaccharide from Angelica sinensis (ASP) as an efficient and safe non-viral gene vector. After modification with branched low molecular weight polyethylenimine (1200 Da), the cationized ASP (cASP) was combined with the plasmid encoding transforming growth factor-beta 1 (TGF-β1) to form a spherical nano-scaled particle (i.e., cASP-pTGF-β1 nanoparticle). This nanoparticle was applied to transfect rat bone marrow mesenchymal stem cells and human umbilical cord mesenchymal stem cells. As a result, nanoparticles (cASP/pDNA weight ratio 10:1) had the greatest transfection efficiency in both cells, which was significantly higher than those of Lipofectamine2000 and PEI (25 kDa). This was in agreement with the findings of the semi-quantitative RT-PCR and live cell imaging. These nanoparticles were also less toxic than Lipofectamine2000 and PEI (25 kDa). Therefore, cASP could be a potential candidate for a novel non-viral gene vector.
  
  
  BACKGROUND
  These authors demonstrate the use of a nanoparticle-based efficient and safe non-viral gene vector delivery system via a spherical nanoparticle based on a polysaccharide from Angelica sinensis, with parameters superior to Lipofectamine2000.
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Jiangsu University, Jingkou District, Zhenjiang, P.R. China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Deng
authors.forename: Min
authors.initials: M
authors.lastname: Fu
authors.forename: Yue
authors.initials: Y
authors.lastname: Cao
authors.forename: Xia
authors.initials: X
authors.lastname: Cao
authors.forename: Miao
authors.initials: M
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.forename: Rui
authors.initials: R
authors.lastname: Qu
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.forename: Ximing
authors.initials: X
authors.lastname: Xu
authors.forename: Jiangnan
authors.initials: J
authors.lastname: Yu
mesh_terms: D029971:Angelica sinensis / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D010957:Plasmids / Q000008:administration & dosage* / Q000235:genetics; D011094:Polyethyleneimine / Q000737:chemistry / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry* / Q000378:metabolism; D051381:Rats; D014162:Transfection / Q000379:methods; D053773:Transforming Growth Factor beta1 / Q000235:genetics
chemical_list: D011134:Polysaccharides; D053773:Transforming Growth Factor beta1; D011094:Polyethyleneimine; D004247:DNA
keywords: Mesenchymal stem cells; Nanoparticle; Non-viral vector; Polyethylenimine; Polysaccharide