title: Cardiotoxin III inhibits proliferation and migration of oral cancer cells through MAPK and MMP signaling.
abstract: Cardiotoxin III (CTXIII), isolated from the snake venom of Formosan cobra Naja naja atra, has previously been found to induce apoptosis in many types of cancer. Early metastasis is typical for the progression of oral cancer. To modulate the cell migration behavior of oral cancer is one of the oral cancer therapies. In this study, the possible modulating effect of CTXIII on oral cancer migration is addressed. In the example of oral squamous carcinoma Ca9-22 cells, the cell viability was decreased by CTXIII treatment in a dose-responsive manner. In wound-healing assay, the cell migration of Ca9-22 cells was attenuated by CTXIII in a dose- and time-responsive manner. After CTXIII treatment, the MMP-2 and MMP-9 protein expressions were downregulated, and the phosphorylation of JNK and p38-MAPK was increased independent of ERK phosphorylation. In conclusion, CTXIII has antiproliferative and -migrating effects on oral cancer cells involving the p38-MAPK and MMP-2/-9 pathways.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Chi-Mei Medical Center, Tainan 710, Taiwan.
authors.forename: Ching-Yu
authors.initials: CY
authors.lastname: Yen
authors.forename: Shih-Shin
authors.initials: SS
authors.lastname: Liang
authors.forename: Lo-Yi
authors.initials: LY
authors.lastname: Han
authors.forename: Han-Lin
authors.initials: HL
authors.lastname: Chou
authors.forename: Chon-Kit
authors.initials: CK
authors.lastname: Chou
authors.forename: Shinne-Ren
authors.initials: SR
authors.lastname: Lin
authors.forename: Chien-Chih
authors.initials: CC
authors.lastname: Chiu
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004179:Cobra Cardiotoxin Proteins / Q000008:administration & dosage*; D005887:Gingival Neoplasms / Q000378:metabolism* / Q000473:pathology*; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D020782:Matrix Metalloproteinases / Q000378:metabolism*
chemical_list: D004179:Cobra Cardiotoxin Proteins; C082202:cardiotoxin III, Naja naja atra; D020782:Matrix Metalloproteinases