title: Expression and secretion of cholera toxin B subunit in lactobacilli.
abstract: Lactic acid bacteria (LAB) are used in various fields, including in food and medical supplies. There has been a great deal of research into vaccine development using LAB as carriers due to their "generally recognized as safe" status. Cholera is an infectious disease that causes diarrhea due to cholera toxin (CT) produced by Vibrio cholerae. The pentameric cholera toxin B (CTB) subunit has no toxicity, and is used as an antigen in cholera vaccines and as a delivery molecule in vaccines to various diseases. In this study, we generated recombinant LAB expressing and secreting CTB. Here, we first report that CTB expressed and secreted from LAB bound to GM1 ganglioside. The secreted CTB was purified, and its immunogenicity was determined by intranasal administration into mice. The results of the present study suggested that it may be useful as the basis of a new oral cholera vaccine combining LAB and CTB.
authors.affiliation: Faculty of Pharmaceutical Sciences, Fukuoka University, Fukuoka, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Okuno
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Kashige
authors.forename: Tomomitsu
authors.initials: T
authors.lastname: Satho
authors.forename: Keiichi
authors.initials: K
authors.lastname: Irie
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Hiramatsu
authors.forename: Tanjina
authors.initials: T
authors.lastname: Sharmin
authors.forename: Yuki
authors.initials: Y
authors.lastname: Fukumitsu
authors.forename: Saori
authors.initials: S
authors.lastname: Uyeda
authors.forename: Seitaro
authors.initials: S
authors.lastname: Yamada
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Harakuni
authors.forename: Takeshi
authors.initials: T
authors.lastname: Miyata
authors.forename: Takeshi
authors.initials: T
authors.lastname: Arakawa
authors.forename: Masumi
authors.initials: M
authors.lastname: Imoto
authors.forename: Akihisa
authors.initials: A
authors.lastname: Toda
authors.forename: Yukihiko
authors.initials: Y
authors.lastname: Nakashima
authors.forename: Fumio
authors.initials: F
authors.lastname: Miake
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000276:immunology / Q000378:metabolism*; D002772:Cholera Toxin / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D022121:Cholera Vaccines / Q000008:administration & dosage; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D005732:Gangliosides / Q000378:metabolism; D007074:Immunoglobulin G / Q000097:blood; D007778:Lactobacillus / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D022121:Cholera Vaccines; D005732:Gangliosides; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D002772:Cholera Toxin