title: Antioxidant effect of butylated hydroxytoluene on ferric nitrilotriacetate induced renal hyper proliferation and injury in rats.
abstract: This study was designed to investigate the effect of butylated hydroxy toluene (BHT), a phenolic antioxidant used in foods, cosmetics and pharmaceutical products, on Fe-NTA-induced nephrotoxicity in rats. Fe-NTA (alone) treatment enhances ornithine decarboxylase activity to 5.3-fold, and [(3)H] thymidine incorporation in DNA to 3.5-fold compared with the corresponding saline treated control. The enhanced ornithine decarboxylase activity and DNA synthesis showed a reduction to 2.12-2.15-fold respectively at a higher dose of 2 mg BHT/day/animal, compared with the Fe-NTA treated group. Fe-NTA treatment also enhanced the renal microsomal lipid peroxidation to 2.0-fold and decreased the activities of glutathione and antioxidant enzymes to a range of 2.2-2.5-fold in kidney. These changes were reversed significantly in animals receiving a pretreatment of BHT. Present data suggests that BHT can prevent the toxic effects of Fe-NTA and can serve as a potent chemopreventive agent to suppress oxidant-induced tissue injury and nephrotoxicity in rats.
authors.affiliation: Department of Clinical Laboratory Sciences, College of Applied Medical Science, King Saud University, Riyadh, Saudi Arabia. sansar@ksu.edu.sa
authors.forename: S
authors.initials: S
authors.lastname: Ansar
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002084:Butylated Hydroxytoluene / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D005290:Ferric Compounds / Q000633:toxicity*; D007668:Kidney / Q000187:drug effects* / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives* / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D005290:Ferric Compounds; D002084:Butylated Hydroxytoluene; D009571:Nitrilotriacetic Acid; C020326:ferric nitrilotriacetate
keywords: BHT; BUN; CRN; EDTA; Fe-NTA; Ferric nitrilotriacetic acid; H(2)O(2); LDH; ODC; Oxidative stress butylated hydroxy toluene; ROS; blood urea nitrogen; butylated hydroxytoluene; creatinine; ethylene diamine tetra acetic acid; ferric nitrilotrilotriacetate; hydrogen peroxide; lactate dehydrogenases; ornithine decarboxylase; reactive oxygen species; sGOT; sGPT; serum glutamate oxaloacetate transaminases; serum glutamate pyruvate transaminases