title: Silver-containing mesoporous bioactive glass with improved antibacterial properties.
abstract: The aim of the present work is the study of the bacteriostatic/bactericidal effect of a silver-containing mesoporous bioactive glass obtained by evaporation-induced self-assembly and successive thermal stabilization. Samples of the manufactured mesophase were characterized by means of transmission electron microscopy and N₂ adsorption/desorption at 77 K, revealing structural and textural properties similar to SBA-15 mesoporous silica. Glass samples used for bioactivity experiments were put in contact with a standardized, commercially available cell culture medium instead of lab-produced simulated body fluid, and were then characterized by means of X-ray diffraction, field emission scanning electron microscopy and Fourier transform infrared spectroscopy. All these analyses confirmed the development of a hydroxyl carbonate apatite layer on glass particles. Moreover, the investigated mesostructure showed a very good antibacterial effect against S. aureus strain, with a strong evidence of bactericidal activity already registered at 0.5 mg/mL of glass concentration. A hypothesis about the mechanism by which Ag affects the bacterial viability, based on the intermediate formation of crystalline AgCl, was also taken into account. With respect to what already reported in the literature, these findings claim a deeper insight into the possible use of silver-containing bioactive glasses as multifunctional ceramic coatings for orthopedic devices.
authors.affiliation: Centro di Ricerca Interdipartimentale sui Biomateriali, Università Federico II, P.le Tecchio 80, 80125 Naples, Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Gargiulo
authors.forename: Angela Maria
authors.initials: AM
authors.lastname: Cusano
authors.forename: Filippo
authors.initials: F
authors.lastname: Causa
authors.forename: Domenico
authors.initials: D
authors.lastname: Caputo
authors.forename: Paolo Antonio
authors.initials: PA
authors.lastname: Netti
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001672:Biocompatible Materials; D005898:Glass; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D012834:Silver / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects; D014961:X-Ray Diffraction
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D012834:Silver