title: Associations of IL-10 gene polymorphisms with acute myeloid leukemia in Hunan, China.
abstract: We investigated the possible association of interleukin-10 (IL-10) single nucleotide polymorphisms (SNPs) and susceptibility to acute myeloid leukemia (AML) in 115 patients and 137 healthy controls. Genetic analysis of IL-10 SNPs at -819 and -592 was carried out with the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) approach. The IL-10 mRNA expression of AML patients and controls with different genotype was detected by real-time quantitative polymerase chain reaction (RT-PCR). Genetic analysis of IL-10 revealed that the -819AA genotype frequencies and the -819A allele frequencies in the AML group were higher than in the controls (59.1% vs 40.9%; 75.6% vs 63.9%, respectively); there were remarkable differences in -819T/C and -592A/C gene distribution (P<0.05) and the TA haploid frequencies were higher in the AML group (75.6% vs 63.9%, P<0.05). IL-10 mRNA expression in incipient AML patients was obvious higher than the non- tumor group and the remission group (7.78?10-3 vs 2.43?10-3, 3.64?10-3, P<0.05).The study suggested that the haploid TA and genotype TA/TA may be associated with AML in Han people in Hunan province.The IL-10 SNPs at -819 and -592 sites were associated with AML and may affect IL-10 mRNA expression in AML patients.
authors.affiliation: Department of Hematology, The Third Xiangya Hospital of Central South University, Changsha, Hunan, China.
authors.forename: Chen-Jiao
authors.initials: CJ
authors.lastname: Yao
authors.forename: Wei
authors.initials: W
authors.lastname: Du
authors.forename: Hai-Bing
authors.initials: HB
authors.lastname: Chen
authors.forename: Sheng
authors.initials: S
authors.lastname: Xiao
authors.forename: Cheng-Hong
authors.initials: CH
authors.lastname: Wang
authors.forename: Zi-Li
authors.initials: ZL
authors.lastname: Fan
mesh_terms: D016022:Case-Control Studies; D002681:China; D017931:DNA Primers / Q000737:chemistry / Q000235:genetics; D005260:Female; D006238:Haploidy; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000628:therapy; D015810:Linkage Disequilibrium; D008297:Male; D009364:Neoplasm Recurrence, Local / Q000235:genetics* / Q000628:therapy; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011379:Prognosis; D012074:Remission Induction
chemical_list: D017931:DNA Primers; D016753:Interleukin-10