title: Application of 2D BN/SDS-PAGE coupled with mass spectrometry for identification of VDAC-associated protein complexes related to mitochondrial binding sites for type I brain hexokinase.
abstract: Two types of binding sites for hexokinase, designated as Type A or Type B sites, have been shown to coexist on brain mitochondria. The ratio of these sites varies between species. HK1 attaches by reversibly binding to the voltage dependent anion channel (VDAC). Regarding the nature of hexokinase binding sites, we investigated if it was linked to distinct VDAC interactomes. We approached this question by 2D BN/SDS-PAGE of mitochondria, followed by mass spectrometry. Our results are consistent with the possibility that the ratio of Type A/Type B sites is due to differential VDAC interactions in bovine and rat neuronal cells.
authors.affiliation: Department of Basic Sciences, School of Animal Science and Food Engineering, University of São Paulo, Pirassununga, Brazil.
authors.forename: Carla Rossini
authors.initials: CR
authors.lastname: Crepaldi
authors.forename: Phelipe Augusto Mariano
authors.initials: PA
authors.lastname: Vitale
authors.forename: Andrea Cristina
authors.initials: AC
authors.lastname: Tesch
authors.forename: Hélen Julie
authors.initials: HJ
authors.lastname: Laure
authors.forename: José César
authors.initials: JC
authors.lastname: Rosa
authors.forename: Marcelo
authors.initials: M
authors.lastname: de Cerqueira César
mesh_terms: D000818:Animals; D001665:Binding Sites; D015153:Blotting, Western; D001921:Brain / Q000201:enzymology / Q000378:metabolism*; D002417:Cattle; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006593:Hexokinase / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods*; D051381:Rats; D050994:Voltage-Dependent Anion Channels / Q000378:metabolism*
chemical_list: D050994:Voltage-Dependent Anion Channels; D006593:Hexokinase
keywords: BN/SDS PAGE; Hexokinase; Interactome; Mitochondria; VDAC