title: 8-isoprostane levels in serum and bronchoalveolar lavage in idiopathic pulmonary fibrosis and sarcoidosis.
abstract: OBJECTIVE
  Sarcoidosis and idiopathic pulmonary fibrosis (IPF) are both associated with deregulated inflammatory mechanisms partially triggered by aggravated oxidative stress. 8-Isoprostane has been proposed as a reliable marker of oxidative stress, linked to several pulmonary diseases. We aimed to explore differences in 8-isoprostane levels in IPF and sarcoidosis patients, and controls.
  
  
  METHODS
  We included 16 IPF and 55 sarcoidosis patients, as well as 17 controls in the study. 8-Isoprostane levels were measured in serum and in bronchoalveolar lavage (BAL).
  
  
  RESULTS
  Serum 8-isoprostane levels were increased in all patient groups vs controls (p<0.001). The systemic 8-isoprostane concentrations were higher in sarcoidosis patients as compared to IPF subjects and controls (p=0.017 and p<0.001, respectively). IPF patients exhibited increased serum 8-isoprostane levels when compared to controls (p<0.001). Sarcoidosis patients presented significantly increased 8-isoprostane BAL levels when compared to IPF patients (p=0.002).
  
  
  CONCLUSIONS
  Our data indicate that the level of oxidative stress, as reflected by 8-isoprostane concentrations, is enhanced in patients with sarcoidosis, and to a lesser extent, in IPF patients when compared to controls, suggesting a potential implication of redox imbalance in both diseases.
authors.affiliation: Respiratory Medicine Department, School of Medicine, University of Thessaly, Larissa, Greece.
authors.forename: Foteini
authors.initials: F
authors.lastname: Malli
authors.forename: Fotini
authors.initials: F
authors.lastname: Bardaka
authors.forename: Irene
authors.initials: I
authors.lastname: Tsilioni
authors.forename: Eleni
authors.initials: E
authors.lastname: Karetsi
authors.forename: Konstantinos I
authors.initials: KI
authors.lastname: Gourgoulianis
authors.forename: Zoe
authors.initials: Z
authors.lastname: Daniil
mesh_terms: D000368:Aged; D015415:Biomarkers / Q000097:blood / Q000378:metabolism*; D001992:Bronchoalveolar Lavage Fluid; D016022:Case-Control Studies; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism; D005260:Female; D006801:Humans; D054990:Idiopathic Pulmonary Fibrosis / Q000097:blood / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D012507:Sarcoidosis / Q000097:blood / Q000378:metabolism*
chemical_list: D015415:Biomarkers; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost
keywords: BAL; DPLDs; EBC; IPF; Idiopathic pulmonary fibrosis; Isoprostane; Oxidative stress; PFTs; ROS; Sarcoidosis; bronchoalveolar lavage; diffuse parenchymal lung diseases; exhaled breath condensate; idiopathic pulmonary fibrosis; pulmonary function testing; reactive oxygen species