title: [Proteins in sperm and seminal plasma associated with human sperm resistance to cryopreservation].
abstract: OBJECTIVE
  To identify the proteins that could improve the resistance of human sperm to cryopreservation using comparative proteomics.
  
  
  METHODS
  A total of 31 semen samples from 10 donors were divided into a high recovery and a low recovery group. Differentially expressed proteins in sperm and seminal plasma were detected and compared between the two groups by two-dimensional differential gel electrophoresis and mass spectrometry.
  
  
  RESULTS
  Totally, 22 differentially expressed proteins were found in the two groups, 12 seminal plasma proteins, 9 sperm proteins, and 1 belonging to both. These identified proteins were involved in the maturation, movement, energy metabolism, DNA repair and other activities of spermatozoa.
  
  
  CONCLUSIONS
  Many proteins were identified in sperm and seminal plasma that might influence the resistance of human sperm to cryopreservation.
authors.affiliation: Zhejiang Institute of Planned Parenthood Research, Hangzhou, Zhejiang 310012, China. zxz20090722@sohu.com
authors.forename: Xin-Zong
authors.initials: XZ
authors.lastname: Zhang
authors.forename: Cheng-Liang
authors.initials: CL
authors.lastname: Xiong
mesh_terms: D000328:Adult; D015925:Cryopreservation; D006801:Humans; D008297:Male; D040901:Proteomics; D012661:Semen / Q000378:metabolism*; D029589:Seminal Plasma Proteins / Q000378:metabolism*; D013081:Sperm Motility; D013094:Spermatozoa / Q000378:metabolism*; D055815:Young Adult
chemical_list: D029589:Seminal Plasma Proteins