title: Effects of celecoxib on cycle kinetics of gastric cancer cells and protein expression of cytochrome C and caspase-9.
abstract: OBJECTIVE
  This investigation aimed to determine effects of celecoxib on the cell cycle kinetics of the gastric cancer cell line MGC803 and the mechanisms involved by assessing expression of cytochrome C and caspase-9 at the protein level.
  
  
  METHODS
  Cell proliferation of MGC803 was determined by MTT assay after treatment with celecoxib. Apoptosis was assessed using fluorescence staining and cell cycle kinetics by flow cytometry. Western blotting was used to detect the expression of caspase-9 protein and of cytochrome C protein in cell cytosol and mitochondria.
  
  
  RESULTS
  Celecoxib was able to restrain proliferation and induce apoptosis in a dose- and time- dependent manner, inducing G0/G1 cell cycle arrest, release of cytochrome C into the cytosol, and cleavage of pro-caspase-9 into its active form.
  
  
  CONCLUSIONS
  Celecoxib can induce apoptosis in MGC803 cells through a mechanism involving cell cycle arrest, mitochondrial cytochrome C release and caspase activation.
authors.affiliation: Department of Gastroenterology, Zhengzhou People's Hospital Affiliated to Southern Medical University, Zhengzhou, Henan, China.
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Wang
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Niu
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Zhen
authors.initials: Z
authors.lastname: Han
authors.forename: Ying-Jie
authors.initials: YJ
authors.lastname: Ma
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D053453:Caspase 9 / Q000378:metabolism*; D000068579:Celecoxib; D002453:Cell Cycle / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D052246:Cyclooxygenase 2 Inhibitors / Q000494:pharmacology*; D045304:Cytochromes c / Q000378:metabolism*; D005434:Flow Cytometry; D006801:Humans; D007700:Kinetics; D011720:Pyrazoles / Q000494:pharmacology*; D013274:Stomach Neoplasms / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D013449:Sulfonamides / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D052246:Cyclooxygenase 2 Inhibitors; D011720:Pyrazoles; D013449:Sulfonamides; D045304:Cytochromes c; D053453:Caspase 9; D000068579:Celecoxib