title: ABC-cassette transporter 1 (ABCA1) expression in epithelial cells in Chlamydia pneumoniae infection.
abstract: ATP-binding cassette transporter A1 (ABCA1) mediates reverse cholesterol transport and innate immunity response in different cell types. We have investigated the regulation of ABCA1 expression in response to intracellular Chlamydia pneumoniae infection in A549 epithelial lung carcinoma cells. C. pneumoniae infection decreased ABCA1 expression in A549 cells, and the activity of the ABCA1 promoter was decreased. The decreased promoter activity was dependent on its E-box and GnT-box elements of the promoter. Chlamydial growth was decreased in ABCA1-silenced epithelial lung carcinoma cells. These data indicate an important role for ABCA1 in intracellular bacterial infection.
authors.affiliation: Turku Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland. juha.korhonen@utu.fi
authors.forename: Juha T
authors.initials: JT
authors.lastname: Korhonen
authors.forename: Vesa M
authors.initials: VM
authors.lastname: Olkkonen
authors.forename: Riitta
authors.initials: R
authors.lastname: Lahesmaa
authors.forename: Mirja
authors.initials: M
authors.lastname: Puolakkainen
mesh_terms: D064286:ATP Binding Cassette Transporter 1 / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D016993:Chlamydophila pneumoniae / Q000472:pathogenicity*; D004847:Epithelial Cells / Q000378:metabolism* / Q000382:microbiology; D005786:Gene Expression Regulation; D006801:Humans; D008168:Lung / Q000166:cytology / Q000382:microbiology; D011401:Promoter Regions, Genetic; D014158:Transcription, Genetic
chemical_list: C577836:ABCA1 protein, human; D064286:ATP Binding Cassette Transporter 1
keywords: ABCA1; Chlamydia pneumoniae; Epithelial cells