title: Cytotoxic constituents from the leaves of Broussonetia papyrifera.
abstract: OBJECTIVE
  To investigate the chemical constituents from the leaves of Broussonetia papyrifera.
  
  
  METHODS
  The chemical constituents were isolated and purified by macroporous adsorptive resin D101, silica gel, and ODS column chromatography and preparative HPLC. Their structures were elucidated on the basis of 1D and 2D NMR analyses. In addition, their cytotoxic activity against human hepatoma carcinoma cells (HepG-2) were evaluated by the MTT method. Furthermore, RP-HPLC and colorimetric methods were used for the analysis of cosmosiin and total flavonoids.
  
  
  RESULTS
  A new lignan, together with five known compounds were obtained, and their structures were characterized as (+)-pinoresinol-4'-O-β-D-glucopyranosyl-4″-O-β-D-apiofuranoside (1), cosmosiin (2), luteolin-7-O-β-D-glucopyranoside (3), liriodendrin (4), 3, 5, 4'-trihydroxy-bibenzyl-3-O-β-D-glucoside (5), and apigenin-6-C-β-D-glucopyranside (6). Furthermore, RP-HPLC and colorimetric methods were established for the analysis of cosmosiin and total flavonoids.
  
  
  CONCLUSIONS
  Compound 1 was a new lignan, and compounds 5 and 6 were isolated for the first time from the title plant. Compounds 1, 4 and 6 showed definite activities against HepG-2, while the other compounds didn't show inhibitory effects. The optimal harvest time of B. papyrifera (L.) Vent. is September.
authors.affiliation: College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, China.
authors.forename: Xiao-Ku
authors.initials: XK
authors.lastname: Ran
authors.forename: Xiao-Tong
authors.initials: XT
authors.lastname: Wang
authors.forename: Pei-Pei
authors.initials: PP
authors.lastname: Liu
authors.forename: Yu-Xin
authors.initials: YX
authors.lastname: Chi
authors.forename: Bo-Jia
authors.initials: BJ
authors.lastname: Wang
authors.forename: De-Qiang
authors.initials: DQ
authors.lastname: Dou
authors.forename: Ting-Guo
authors.initials: TG
authors.lastname: Kang
authors.forename: Wei
authors.initials: W
authors.lastname: Xiong
mesh_terms: D031623:Broussonetia / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D003603:Cytotoxins / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D056945:Hep G2 Cells; D006801:Humans; D017705:Lignans / Q000737:chemistry / Q000633:toxicity; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000633:toxicity*; D018515:Plant Leaves / Q000737:chemistry*
chemical_list: D003603:Cytotoxins; D017705:Lignans; D010936:Plant Extracts