title: Probing the micellar properties of Quinacrine 2HCl and its binding with surfactants and human serum albumin.
abstract: This manuscript reports physicochemical behavior of an antimalarial drug Quinacrine 2HCl (QUN) drug as well as its interaction with surfactant and Human Serum Albumin (HSA). Surface tension and specific conductivity were employed to detect the critical micelle concentration (CMC) and thus its surface and thermodynamic parameters were calculated. Solublization of this drug within micelles of anionic surfactant sodium dodecyl sulfate (SDS) has also been studied. UV/Visible spectroscopy was used to calculate partition coefficient (Kx), free energy of partition and number of drug molecules per micelle. The complexation of drug with HSA at physiological conditions (pH 7.4) has been analyzed by using UV/Visible and fluorescence spectroscopy. In this way the values of drug-protein binding constant, number of binding sites and free energy of binding were calculated.
authors.affiliation: Department of Chemistry, Government College University, Faisalabad, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Usman
authors.forename: Mohammad
authors.initials: M
authors.lastname: Siddiq
mesh_terms: D000042:Absorption; D000327:Adsorption; D001665:Binding Sites; D004553:Electric Conductivity; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008823:Micelles; D011485:Protein Binding; D011796:Quinacrine / Q000737:chemistry / Q000378:metabolism*; D012709:Serum Albumin / Q000378:metabolism*; D012996:Solutions; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013500:Surface Tension; D013501:Surface-Active Agents / Q000378:metabolism*; D013816:Thermodynamics
chemical_list: D008823:Micelles; D012709:Serum Albumin; D012996:Solutions; D013501:Surface-Active Agents; D011796:Quinacrine
keywords: Conductivity; Drug; Protein; Spectroscopy; Surface tension; Surfactant