title: Analysis of loss of heterozygsity effect on thyroid tumor with oxyphilia cell locus in familial non medullary thyroid carcinoma in Iranian families.
abstract: METHODS
  22 nuclear families (78 persons including 12 patients) with papillary and follicular tumors were selected in a period of six months from Milad hospital. Five microsatellite markers (D19S413, D19S391, D19S916, D19S568, D19S865) on 19p13.2 were selected for genetic analysis. Genomic DNAs was extracted; PCR and polyacrylamide gel electrophoresis method were used for variation detection.
  
  
  RESULTS
  The results show that 5.4% of the follicular carcinomas and 17.9% of the papillary carcinomas presented LOH at recognition sites. LOH of Papillary carcinoma detected about 13.9% and follicular carcinoma 7.2% in this study. The frequency of informative cases was not similar for each marker: D19S413 (41.1%)[1], D19S391 (12.5%), D19S916 (10.7%), D19S568 (1.8%) and D19S865 (3.6%). Loss of hetrozygosity in D19S413 predicts the relation between variation in this region and the disease.
  
  
  CONCLUSIONS
  Our findings showed an average of 13.9% LOH in FNMTC cases. Among the five major microsatellites, D19S413 was the most informative for LOH analysis of FNMTC.
authors.affiliation: Cellular Molecular and Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Hasti Atashi
authors.initials: HA
authors.lastname: Shirazi
authors.forename: Mehdi
authors.initials: M
authors.lastname: Hedayati
authors.forename: Maryam Sadat
authors.initials: MS
authors.lastname: Daneshpour
authors.forename: Abdollah
authors.initials: A
authors.lastname: Shafiee
authors.forename: Fereidoun
authors.initials: F
authors.lastname: Azizi
keywords: Familial non medullary thyroid carcinoma; Iran; loss of heterozygosis; thyroid tumor with oxyphilia cell