title: Biocontrol of late blight and plant growth promotion in tomato using rhizobacterial isolates.
abstract: Seven bacterial isolates (viz., AB05, AB10, AB11, AB12, AB14, AB15, and AB17) were derived from the rhizosphere and evaluated in terms of plant growth-promoting activities and the inhibition of Phytophthora infestans affecting tomatoes in Korea. According to 16S rDNA sequencing, a majority of the isolates are members of Bacillus, and a single isolate belongs to Paenibacillus. All seven isolates inhibited P. infestans by more than 60% in vitro. However, AB15 was the most effective, inhibiting mycelial growth of the pathogen by more than 80% in vitro and suppressing disease by 74% compared with control plants under greenhouse conditions. In a PGPR assay, all of the bacterial isolates were capable of enhancing different growth parameters (shoot/root length, fresh biomass, dry matter, and chlorophyll content) in comparison with non-inoculated control plants. AB17-treated plants in particular showed the highest enhancement in fresh biomass with 18% and 26% increments in the root and shoot biomass, respectively. However, isolate AB10 showed the highest shoot and root growth with 18% and 26% increments, respectively. Moreover, the total chlorophyll content was 14%~19% higher in treated plants.
authors.affiliation: Department of Applied Plant Sciences, Kangwon National University, Chuncheon 200-701, Korea.
authors.forename: Kabir
authors.initials: K
authors.lastname: Lamsal
authors.forename: Sang Woo
authors.initials: SW
authors.lastname: Kim
authors.forename: Yun Seok
authors.initials: YS
authors.lastname: Kim
authors.forename: Youn Su
authors.initials: YS
authors.lastname: Lee
mesh_terms: D001407:Bacillus / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D018533:Biomass; D002734:Chlorophyll / Q000032:analysis; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D007723:Korea; D018551:Solanum lycopersicum / Q000254:growth & development* / Q000382:microbiology*; D056265:Microbial Interactions; D056507:Paenibacillus / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification; D010572:Pest Control, Biological / Q000379:methods*; D010802:Phylogeny; D055750:Phytophthora infestans / Q000254:growth & development; D010935:Plant Diseases / Q000517:prevention & control*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D002734:Chlorophyll