title: Crystallization and preliminary structural analysis of dibenzothiophene monooxygenase (DszC) from Rhodococcus erythropolis.
abstract: Dibenzothiophene (DBT) and its derivatives are typical sulfur compounds found in fossil fuels. These compounds show resistance to the hydrodesulfurization treatment that is commonly used in industry. Dibenzothiophene monooxygenase (DszC) is responsible for the oxidation of DBT, which is the first and the rate-limiting step in the DBT enzymatic desulfurization 4S pathway. In this study, the crystal structure of DszC from Rhodococcus erythropolis DS-3 is reported. The crystal of native DszC belonged to space group P1, with unit-cell parameters a = 96.16, b = 96.27, c = 98.56 Å, α = 81.03, β = 67.57, γ = 85.84°. To determine the phase, SAD X-ray diffraction data were collected from a SeMet-derivative DszC crystal, which also belonged to space group P1, with unit-cell parameters a = 95.379, b = 95.167, c = 94.891 Å, α = 87.046, β = 70.536, γ = 79.738°. Further structural analysis of DszC is in progress.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin 300071, People's Republic of China.
authors.forename: Xiaolu
authors.initials: X
authors.lastname: Duan
authors.forename: Liang
authors.initials: L
authors.lastname: Zhang
authors.forename: Daming
authors.initials: D
authors.lastname: Zhou
authors.forename: Kaihua
authors.initials: K
authors.lastname: Ji
authors.forename: Ting
authors.initials: T
authors.lastname: Ma
authors.forename: Wenqing
authors.initials: W
authors.lastname: Shui
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Li
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000302:isolation & purification; D003460:Crystallization; D010088:Oxidoreductases / Q000737:chemistry* / Q000302:isolation & purification*; D017433:Protein Structure, Secondary; D012240:Rhodococcus / Q000201:enzymology*; D014961:X-Ray Diffraction
chemical_list: D001426:Bacterial Proteins; D010088:Oxidoreductases; C090100:dibenzothiophene monooxygenase
keywords: DszC; Rhodococcus erythropolis; SAD