title: Synthesis and characterization of chitosan tripolyphosphate nanoparticles and its encapsulation efficiency containing Russell's viper snake venom.
abstract: Chitosan Tripolyphosphate (CS/TPP) nanoparticle is a biodegradable and nontoxic polysaccharide, used as a carrier for drug delivery. The morphology and particle-size measurements of the nanoparticles were studied by field emission scanning electron microscopy and Fourier Transform Infrared Spectroscopy (FTIR). This study aims to evaluate the impact of Russell's viper venom encapsulation on various factors and loading capacity, in addition to explore the physicochemical structure of nanoparticles. FTIR confirmed that tripolyphosphoric groups of TPP linked with ammonium groups of CS in the nanoparticles. Our results showed that CS can react with TPP to form stable cationic nanoparticles. The results also showed that encapsulation efficiency of venom at different concentrations of 20, 40, 60, 500, and 1000 µg/mL were achieved for CS/TPP nanoparticles at different concentrations of 1.5, 2, and 3 mg/mL. The cytotoxicity of CS/TPP nanoparticles was evaluated by MTT (-3 (4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide, a tetrazole) assay.
authors.affiliation: Aquaculture Biotechnology Division, Department of Zoology, C.Abdul Hakeem College, Melvisharam 632 509, Vellore, India.
authors.forename: C
authors.initials: C
authors.lastname: Venkatesan
authors.forename: S
authors.initials: S
authors.lastname: Vimal
authors.forename: A S Sahul
authors.initials: AS
authors.lastname: Hameed
mesh_terms: D000818:Animals; D048271:Chitosan / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D016503:Drug Delivery Systems; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D017840:Daboia; D014757:Viper Venoms / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D014757:Viper Venoms; D048271:Chitosan
keywords: Chitosan Tripolyphosphate; Fourier Transform Infrared Spectra; Nanoparticle; Russell's Viper