title: [Study on identification of wild Dipsacus asper and cultivated Dipsacus asper at different growth years by organization structure].
abstract: OBJECTIVE
  To study the regular pattern of growing development and the structural changes of the main root of Dipsacus asper; Find the basis for distinguishing between wild and cultivated Dipsacus asper as well as identifying cultivated Dipsacus asper at different growth years.
  
  
  METHODS
  Continuous sectional sampling and histological observation was conducted on the main roots of annual and biennial cultivated Dipsacus asper and on the main roots of bolting and non-bolting plants of wild Dipsacus asper; Histological observation was carried out respectively on the main roots of one to four-year-old cultivated Dipsacus asper.
  
  
  RESULTS
  It was discovered for the first time that there was structure of growth rings in the main roots of Dipsacus asper. And the ways in which the vessels of the growth rings were arranged differed between wild and cultivated Dipsacus asper, the vessels of growth rings in wild Dipsacus asper were arranged tangentially while those in cultivated Dipsacus asper were arranged as clusters. Pith existed in the upper part of roots of wild Dipsacus asper,while it was not found in the cultivated ones, and this could be adopted as a preliminary method to distinguish the wild Dipsacus asper from cultivatedone. The structures of growth rings in each main root were in consistency from top to bottom, and the thickening of roots mainly depended on the growth of xylem in the first year. There were particular relations and regular patterns between the structure of main roots of one to four-year-old cultivated Dipsacus asper and the ages of their growth, which could serve as the primary basis for identifying their growth years.
  
  
  CONCLUSIONS
  Methods to distinguish wild Dipsacus asper from cultivated ones at different ages by organization structure are established, which could provide the basis for identification of the medicinal material of Dipsacus asper.
authors.affiliation: Anhui Key Laboratory of Modernized Chinese Material Medica, Department of Pharmacy, Anhui University of Traditional Chinese Medicine, Hefei 230031, China. 1094704506@qq.com
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Hua-Sheng
authors.initials: HS
authors.lastname: Peng
authors.forename: Yan
authors.initials: Y
authors.lastname: Xuan
authors.forename: Jie
authors.initials: J
authors.lastname: Qu
mesh_terms: D049917:Dipsacaceae / Q000033:anatomy & histology* / Q000254:growth & development*; D008856:Microscopy, Fluorescence; D018517:Plant Roots / Q000033:anatomy & histology* / Q000254:growth & development; D010946:Plants, Medicinal / Q000033:anatomy & histology / Q000254:growth & development; D013997:Time Factors; D052584:Xylem / Q000033:anatomy & histology / Q000254:growth & development