title: The dynamics of polycomb group proteins in early embryonic nervous system in mouse and human.
abstract: Polycomb group (PcG) proteins are transcription regulatory proteins that control the expression of a variety of genes and the antero-posterior neural patterning from early embryogenesis. Although expression of PcG genes in the nervous system has been noticed, but the expression pattern of PcG proteins in early embryonic nervous system is still unclear. In this study, we analyzed the expression pattern of PRC1 complex members (BMI-1 and RING1B) and PRC2 complex members (EED, SUZ12 and EZH2) in early embryonic nervous system in mouse and human by Western blot and Immunohistochemistry. The results of Western blot showed that EED protein was significantly up-regulated with the increase of the day of pregnancy during the early embryogenesis in mouse. BMI-1 protein level was significantly increased from the day 10 of pregnancy, when compared with the day 9 of pregnancy. But the SUZ12, EZH2 and RING1B protein level did not change significantly. From the results of Immunohistochemistry, we found that the four PcG proteins were all expressed in the fetal brain and fetal spinal cord in mouse. In human, the expression of EED, SUZ12, and EZH2 was not significantly different in cerebral cortex and sacral spinal cord, but BMI-1 and RING1B expression was enhanced with the development of embryos in early pregnancy. Collectively, our findings showed that PRC1 and PRC2 were spatiotemporally expressed in brain and spinal cord of early embryos.
authors.affiliation: Chongqing Key Laboratory of Birth Defects and Reproductive Health, Chongqing, China; Graduate Schools, Peking Union Medical College, Beijing, China; Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Qi
authors.forename: Jing-Li
authors.initials: JL
authors.lastname: Cao
authors.forename: Yi
authors.initials: Y
authors.lastname: Hu
authors.forename: Ji-Gao
authors.initials: JG
authors.lastname: Yang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Ji
authors.forename: Jing
authors.initials: J
authors.lastname: Huang
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Da-Guang
authors.initials: DG
authors.lastname: Sun
authors.forename: Hong-Fei
authors.initials: HF
authors.lastname: Xia
authors.forename: Xu
authors.initials: X
authors.lastname: Ma
mesh_terms: D000367:Age Factors; D000818:Animals; D019521:Body Patterning / Q000502:physiology*; D018797:Cell Cycle Proteins / Q000378:metabolism; D004622:Embryo, Mammalian; D005260:Female; D005333:Fetus; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D006801:Humans; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009420:Nervous System / Q000196:embryology* / Q000378:metabolism*; D063150:Polycomb Repressive Complex 1 / Q000378:metabolism; D063146:Polycomb-Group Proteins / Q000378:metabolism*; D011247:Pregnancy
chemical_list: C069077:BMI1 protein, human; D018797:Cell Cycle Proteins; C116662:PRC1 protein, human; D063146:Polycomb-Group Proteins; D063150:Polycomb Repressive Complex 1; C489841:RNF2 protein, human
keywords: Human; Mouse; Neurogenesis; PRC1; PRC2