title: Preparation, characterization and biological test of 3D-scaffolds based on chitosan, fibroin and hydroxyapatite for bone tissue engineering.
abstract: This work describes the preparation and characterization of porous 3D-scaffolds based on chitosan (CHI), chitosan/silk fibroin (CHI/SF) and chitosan/silk fibroin/hydroxyapatite (CHI/SF/HA) by freeze drying. The biomaterials were characterized by X-ray diffraction, attenuated total reflection Fourier transform infrared spectroscopy, thermogravimetric analysis, differential scanning calorimetry, scanning electron microscopy and energy dispersive spectroscopy. In addition, studies of porosity, pore size, contact angle and biological response of SaOs-2osteoblastic cells were performed. The CHI scaffolds have a porosity of 94.2±0.9%, which is statistically higher than the one presented by CHI/SF/HA scaffolds, 89.7±2.6%. Although all scaffolds were able to promote adhesion, growth and maintenance of osteogenic differentiation of SaOs-2 cells, the new 3D-scaffold based on CHI/SF/HA showed a significantly higher cell growth at 7 days and 21 days and the level of alkaline phosphatase at 14 and 21 days was statistically superior compared to other tested materials.
authors.affiliation: Departamento de Ciências de Materiais, Universidade Federal de Sergipe, São Cristóvão, Sergipe, Brazil.
authors.forename: Paulo Autran Leite
authors.initials: PA
authors.lastname: Lima
authors.forename: Cristiane Xavier
authors.initials: CX
authors.lastname: Resende
authors.forename: Glória Dulce de Almeida
authors.initials: GD
authors.lastname: Soares
authors.forename: Karine
authors.initials: K
authors.lastname: Anselme
authors.forename: Luís Eduardo
authors.initials: LE
authors.lastname: Almeida
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D005349:Fibroins / Q000737:chemistry*; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis; D016062:Porosity; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D005349:Fibroins; D048271:Chitosan; D017886:Durapatite