title: Analysis of herb-herb interaction when decocting together by using ultra-high-performance liquid chromatography-tandem mass spectrometry and fuzzy chemical identification strategy with poly-proportion design.
abstract: A novel and generally applicable approach was established for the herb-herb interaction analysis when decocting together by using ultra-high-performance liquid chromatography coupled with a triple quadrupole electrospray tandem mass spectrometer and fuzzy chemical identification with poly-proportion design. A simple programme was originally developed for the rapid identification and classification of herbal constituents on the basis of the establishment of herbal constituent databases, recognition of the reference compound peaks, selection of the diagnostic ions or fragmentation pathways, classification of chemical groups and formation of group networks. In this study, the exact structures of the chemical constituents did not need to be determined, and only the constituents attributed to different groups were further considered for quantitative analysis. Such a novel approach was successfully applied to kansui-licorice interaction analysis when decocting together. A total of 26 constituents from kansui and 45 constituents from licorice were classified into different chemical groups, and they were further quantitatively analyzed on the basis of semi-symmetric proportion design. The results showed that kansui could significantly promote the concentration of most triterpenoid saponins, phenylpropanoids and their glycosides (the constituents from licorice) in solution when co-decocting, and licorice could clearly promote the concentration of most diterpenes and triterpenes (the constituents from kansui) in solution, potentially explaining the incompatibility of kansui and licorice. Overall, the presently developed strategy should be useful for the interaction analysis for complex mixtures containing various complicated constituents, such as herbal, environmental, agricultural and biological samples.
authors.affiliation: Jiangsu Key Laboratory for High Technology of TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210023, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Shen
authors.forename: Xuan
authors.initials: X
authors.lastname: Mo
authors.forename: Yuping
authors.initials: Y
authors.lastname: Tang
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Hanqing
authors.initials: H
authors.lastname: Pang
authors.forename: Yefei
authors.initials: Y
authors.lastname: Qian
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Chen
authors.forename: Weiwei
authors.initials: W
authors.lastname: Tao
authors.forename: Sheng
authors.initials: S
authors.lastname: Guo
authors.forename: Erxin
authors.initials: E
authors.lastname: Shang
authors.forename: Shaoqing
authors.initials: S
authors.lastname: Zhu
authors.forename: Yunhe
authors.initials: Y
authors.lastname: Ding
authors.forename: Jianming
authors.initials: J
authors.lastname: Guo
authors.forename: Pei
authors.initials: P
authors.lastname: Liu
authors.forename: Shulan
authors.initials: S
authors.lastname: Su
authors.forename: Dawei
authors.initials: D
authors.lastname: Qian
authors.forename: Jin-ao
authors.initials: JA
authors.lastname: Duan
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004347:Drug Interactions; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D028481:Euphorbia / Q000737:chemistry; D006027:Glycosides / Q000737:chemistry* / Q000302:isolation & purification; D006035:Glycyrrhiza / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D015203:Reproducibility of Results; D012107:Research Design; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014315:Triterpenes / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D004365:Drugs, Chinese Herbal; D006027:Glycosides; D010936:Plant Extracts; D014315:Triterpenes