title: [Simultaneous determination of ephedrine hydrochloride, D-pseudoephedrine and amygdalin in xiao'er pingchuan qutan granule by HPLC].
abstract: OBJECTIVE
  To establish an HPLC method for the determination of ephedrine hydrochloride, D-pseudo-ephedrine and amygdalin in Xiao'er Pingchuan Qutan granule.
  
  
  METHODS
  Pheny ether chromatographic column (4.6 mm x 250 mm, 5 microm) was adopted, with acetonitrile-0.1% phosphoric acid (containing 0.1% three ethylamine) (3:97) as the mobile phase. The UV detection wavelength was at 210 nm, with the flow rate of 1 mL x min(-1), and column temperature was at 35 degrees C.
  
  
  RESULTS
  The linearity of ephedrine hydrochloride, D-pseudo-ephedrine and amygdalin ranged between 0.078 60-3.144 microg (r = 1.000 0), 0.103 4-2.068 microg (r = 0.999 7) and 0.430 5-3.157 microg (r = 0.999 8), respectively. Their average recoveries were 98.46% (RSD 1.1%), 103.0% (RSD 1.5%) and 97.15% (RSD 2.1%), respectively.
  
  
  CONCLUSIONS
  The method is simple, stable and reliable that it can be used to determine the content of ephedrine hydrochloride, D-pseudo-ephedrine and amygdalin in Xiao'er Pingchuan Qutan granule.
authors.affiliation: Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. 641026952@qq.com
authors.forename: De-Bin
authors.initials: DB
authors.lastname: Yang
authors.forename: Yan
authors.initials: Y
authors.lastname: Tong
authors.forename: Zhen-Shan
authors.initials: ZS
authors.lastname: Ma
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Mei-Hong
authors.initials: MH
authors.lastname: Dong
authors.forename: Yan-Ling
authors.initials: YL
authors.lastname: Li
authors.forename: Jin-Yu
authors.initials: JY
authors.lastname: Wang
mesh_terms: D000678:Amygdalin / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D004809:Ephedrine / Q000032:analysis* / Q000737:chemistry; D016014:Linear Models; D054199:Pseudoephedrine / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D013997:Time Factors
chemical_list: D004365:Drugs, Chinese Herbal; D000678:Amygdalin; D054199:Pseudoephedrine; D004809:Ephedrine