title: Sequence determination and analysis of three plasmids of Pseudomonas sp. GLE121, a psychrophile isolated from surface ice of Ecology Glacier (Antarctica).
abstract: Pseudomonas sp. GLE121 (a psychrophilic Antarctic strain) carries three plasmids: pGLE121P1 (6899 bp), pGLE121P2 (8330 bp) and pGLE121P3 (39,583 bp). Plasmids pGLE121P1 and pGLE121P2 show significant sequence similarity to members of the IncP-9 and IncP-7 incompatibility groups, respectively, while the largest replicon, pGLE121P3, is highly related to plasmid pNCPPB880-40 of Pseudomonas syringae pathovar tomato NCPPB880. All three plasmids have a narrow host range, limited to members of the genus Pseudomonas. Plasmid pGLE121P3 encodes a conjugal transfer system, while pGLE121P1 carries only a putative MOB module, conserved in many mobilizable plasmids. Plasmid pGLE121P3 contains an additional load of genetic information, including a pair of genes with homology to the rulAB operon, responsible for ultraviolet radiation (UVR) tolerance. Given the increasing UV exposure in Antarctic regions, the expression of these genes is likely to be an important adaptive response.
authors.affiliation: University of Warsaw, Faculty of Biology, Institute of Microbiology, Department of Bacterial Genetics, Miecznikowa 1, 02-096 Warsaw, Poland. ldziewit@biol.uw.edu.pl
authors.forename: Lukasz
authors.initials: L
authors.lastname: Dziewit
authors.forename: Jakub
authors.initials: J
authors.lastname: Grzesiak
authors.forename: Anna
authors.initials: A
authors.lastname: Ciok
authors.forename: Marta
authors.initials: M
authors.lastname: Nieckarz
authors.forename: Marek K
authors.initials: MK
authors.lastname: Zdanowski
authors.forename: Dariusz
authors.initials: D
authors.lastname: Bartosik
mesh_terms: D000864:Antarctic Regions; D001483:Base Sequence; D019295:Computational Biology; D052978:Disk Diffusion Antimicrobial Tests; D022761:Gene Transfer, Horizontal / Q000235:genetics; D046448:Ice Cover / Q000382:microbiology*; D008969:Molecular Sequence Data; D018933:Pili, Sex / Q000235:genetics; D010957:Plasmids / Q000235:genetics*; D011549:Pseudomonas / Q000235:genetics*; D017422:Sequence Analysis, DNA
keywords: Antarctica; Horizontal gene transfer; Plasmid; Pseudomonas sp. GLE121; Psychrophile