title: Pseudomonas reactans, a bacterial strain isolated from the intestinal flora of Blattella germanica with anti-Beauveria bassiana activity.
abstract: Anti-Beauveria bassiana activity of aqueous fecal extracts from conventional German cockroaches [Blattella germanica (L.)] was detected, but was not detected in samples from germ-free German cockroaches. Subsequently, bacterial strain BGI-14 was isolated from the gut of conventional German cockroaches and was identified as Pseudomonas reactans based on 16S rDNA sequence. The strain BGI-14 not only inhibited the germination of conidia, but also inhibited the growth of B. bassiana hyphae. Further studies demonstrated that B. bassiana infections in German cockroaches orally treated with the extracts of BGI-14 fermentation were significantly weakened. Compared with the control group, the cumulative mortality rate of treatment group was reduced by 10.3% at 20 d postinoculation. These studies imply that intestinal flora with anti-B. bassiana activity might contribute to resistance of infection by entomopathogenic fungi.
authors.affiliation: Key Laboratory of Animal Resistance Research, College of Life Science, Shandong Normal University, 88 East Wenhua Rd., Jinan 250014, PR China.
authors.forename: Fan
authors.initials: F
authors.lastname: Zhang
authors.forename: Yan Hong
authors.initials: YH
authors.lastname: Huang
authors.forename: Shu Zhen
authors.initials: SZ
authors.lastname: Liu
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Bo Tai
authors.initials: BT
authors.lastname: Li
authors.forename: Xiao Xu
authors.initials: XX
authors.lastname: Zhao
authors.forename: Ying
authors.initials: Y
authors.lastname: Fu
authors.forename: Jian Jun
authors.initials: JJ
authors.lastname: Liu
authors.forename: Xue Xia
authors.initials: XX
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D052982:Beauveria / Q000187:drug effects*; D020048:Blattellidae / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D004275:DNA, Ribosomal / Q000235:genetics / Q000378:metabolism; D005243:Feces / Q000382:microbiology; D041981:Gastrointestinal Tract / Q000382:microbiology; D005856:Germ-Free Life; D025301:Hyphae / Q000187:drug effects; D008297:Male; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011549:Pseudomonas / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013172:Spores, Fungal / Q000187:drug effects
chemical_list: D000935:Antifungal Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal