title: Dot immunogold filtration assay (DIGFA) for the rapid detection of specific antibodies against the rat lungworm Angiostrongylus cantonensis (Nematoda: Metastrongyloidea) using purified 31-kDa antigen.
abstract: A rapid dot immunogold filtration assay (DIGFA) was adopted for specific immunodiagnosis of human cerebral angiostrongyliasis, using purified 31-kDa glycoprotein specific to Angiostrongylus cantonensis as diagnostic antigen and protein A colloidal gold conjugate as antigen-antibody detector. A total of 59 serum samples were assayed - 11 samples from clinically diagnosed patients with detectable A. cantonensis-specific antibody in immunoblotting; 23 samples from patients with other related parasitic diseases, i.e. gnathostomiasis (n= 8), cysticercosis (n= 5), toxocariasis (n= 2), filariasis (n= 4), paragonimiasis (n= 2) and malaria (n= 2); and 25 samples from normal healthy subjects. The sensitivity and specificity of DIGFA to detect anti-A. cantonensis specific antibodies in serologically confirmed angiostrongyliasis cases, were both 100%. No positive DIGFA was observed in cases with other parasitic diseases, and the healthy control subjects. The 3-min DIGFA is as sensitive and specific as the 3-h immunoblot test in angiostrongyliasis confirmed cases that revealed a 31-kDa reactive band. The gold-based DIGFA is more rapid and easier to perform than the traditional enzyme-linked immunosorbent assay (ELISA). The test utilizing purified A. cantonensis antigen is reliable and reproducible for specific immunodiagnosis of human infection with A. cantonensis - thus can be applied as an additional routine test for clinical diagnostic support. Large-scale sero-epidemiological studies in endemic communities in north-east Thailand are under way to evaluate its usefulness under field conditions.
authors.affiliation: Department of Parasitology, Faculty of Medicine Siriraj Hospital,Mahidol University,Bangkok10700,Thailand.
authors.forename: P
authors.initials: P
authors.lastname: Eamsobhana
authors.affiliation: Institute of Parasitic Diseases, Zhejiang Academy of Medical Sciences,Hangzhou310013,PR China.
authors.forename: X X
authors.initials: XX
authors.lastname: Gan
authors.affiliation: Institute of Parasitic Diseases, Zhejiang Academy of Medical Sciences,Hangzhou310013,PR China.
authors.forename: A
authors.initials: A
authors.lastname: Ma
authors.affiliation: Institute of Parasitic Diseases, Zhejiang Academy of Medical Sciences,Hangzhou310013,PR China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Parasitology, Faculty of Medicine Siriraj Hospital,Mahidol University,Bangkok10700,Thailand.
authors.forename: D
authors.initials: D
authors.lastname: Wanachiwanawin
authors.affiliation: Institute of Biological Sciences, University of Malaya,50603Kuala Lumpur,Malaysia.
authors.forename: H S
authors.initials: HS
authors.lastname: Yong
mesh_terms: D017185:Angiostrongylus cantonensis / Q000276:immunology*; D000818:Animals; D000909:Antibodies, Helminth / Q000276:immunology*; D000918:Antibody Specificity; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D007150:Immunohistochemistry / Q000379:methods*; D012680:Sensitivity and Specificity; D017206:Strongylida Infections / Q000175:diagnosis / Q000276:immunology / Q000469:parasitology
chemical_list: D000909:Antibodies, Helminth