title: The interaction between LYVE-1 with hyaluronan on the cell surface may play a role in the diversity of adhesion to cancer cells.
abstract: Hyaluronan (HA), a simple disaccharide unit, can polymerize and is considered a primary component of the extracellular matrix, which has a wide range of biological functions. In recent years, HA was found on the surface of tumor cells. According to previous reports, differing HA content on the cell surface of tumor cells is closely related to lymph node metastases, but the mechanisms mediating this process remained unclear. This research intended to study the surface content of HA on tumor cells and analyze cell adhesive changes caused by the interaction between HA and its lymphatic endothelial receptor (LYVE-1). We screened and observed high HA content on HS-578T breast cells and low HA content on MCF-7 breast cells through particle exclusion, immunofluorescence and flow cytometry experiments. The expression of LYVE-1, the lymph-vessel specific HA receptor, was consistent with our previous report and enhanced the adhesion of HA(high)-HS-578T cells to COS-7(LYVE-1(+)) through HA in cell static adhesion and dynamic parallel plate flow chamber experiments. MCF-7 breast cells contain little HA on the surface; however, our results showed little adhesion difference between MCF-7 cells and COS-7(LYVE-1(+)) and COS-7(LYVE-1(-)) cells. Similar results were observed concerning the adhesion of HS-578T cells or MCF-7 cells to SVEC4-10 cells. Furthermore, we observed for the first time that the cell surface HA content of high transfer tumor cells was rich, and we visualized the cross-linking of HA cable structures, which may activate LYVE-1 on lymphatic endothelial cells, promoting tumor adhesion. In summary, high-low cell surface HA content of tumor cells through the interaction with LYVE-1 leads to adhesion differences.
authors.affiliation: Department of Molecular Biology Laboratory, Shanghai Sixth People's Hospital Affiliated with Shanghai Jiaotong University, Shanghai, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Du
authors.forename: Hua
authors.initials: H
authors.lastname: Liu
authors.forename: Yiqing
authors.initials: Y
authors.lastname: He
authors.forename: Yiwen
authors.initials: Y
authors.lastname: Liu
authors.forename: Cuixia
authors.initials: C
authors.lastname: Yang
authors.forename: Muqing
authors.initials: M
authors.lastname: Zhou
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Wang
authors.forename: Lian
authors.initials: L
authors.lastname: Cui
authors.forename: Jiajie
authors.initials: J
authors.lastname: Hu
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
mesh_terms: D000818:Animals; D019556:COS Cells; D002448:Cell Adhesion / Q000502:physiology*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism* / Q000502:physiology; D002522:Chlorocebus aethiops; D042783:Endothelial Cells / Q000378:metabolism / Q000502:physiology; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D006820:Hyaluronic Acid / Q000378:metabolism*; D042601:Lymphatic Vessels / Q000378:metabolism / Q000502:physiology; D061986:MCF-7 Cells; D033921:Vesicular Transport Proteins / Q000378:metabolism*
chemical_list: D018960:Hyaluronan Receptors; C119248:LYVE1 protein, human; D033921:Vesicular Transport Proteins; D006820:Hyaluronic Acid