title: Effects of exercise-induced intracellular acidosis on the phosphocreatine recovery kinetics: a 31P MRS study in three muscle groups in humans.
abstract: Little is known about the metabolic differences that exist among different muscle groups within the same subjects. Therefore, we used (31)P-magnetic resonance spectroscopy ((31)P-MRS) to investigate muscle oxidative capacity and the potential effects of pH on PCr recovery kinetics between muscles of different phenotypes (quadriceps (Q), finger (FF) and plantar flexors (PF)) in the same cohort of 16 untrained adults. The estimated muscle oxidative capacity was lower in Q (29 ± 12 mM min(-1), CV(inter-subject) = 42%) as compared with PF (46 ± 20 mM min(-1), CV(inter-subject) = 44%) and tended to be higher in FF (43 ± 35 mM min(-1), CV(inter-subject) = 80%). The coefficient of variation (CV) of oxidative capacity between muscles within the group was 59 ± 24%. PCr recovery time constant was correlated with end-exercise pH in Q (p < 0.01), FF (p < 0.05) and PF (p < 0.05) as well as proton efflux rate in FF (p < 0.01), PF (p < 0.01) and Q (p = 0.12). We also observed a steeper slope of the relationship between end-exercise acidosis and PCr recovery kinetics in FF compared with either PF or Q muscles. Overall, this study supports the concept of skeletal muscle heterogeneity by revealing a comparable inter- and intra-individual variability in oxidative capacity across three skeletal muscles in untrained individuals. These findings also indicate that the sensitivity of mitochondrial respiration to the inhibition associated with cytosolic acidosis is greater in the finger flexor muscles compared with locomotor muscles, which might be related to differences in permeability in the mitochondrial membrane and, to some extent, to proton efflux rates.
authors.affiliation: Centre de Resonance Magnetique Biologique et Medicale, UMR CNRS 6612, Faculté de Médecine de Marseille, Marseille, France; Department of Medicine, Division of Geriatrics, University of Utah, Salt Lake City, UT, USA; Geriatric Research, Education, and Clinical Center, George E. Whalen VA Medical Center, Salt Lake City, UT, USA.
authors.forename: Gwenael
authors.initials: G
authors.lastname: Layec
authors.forename: Emil
authors.initials: E
authors.lastname: Malucelli
authors.forename: Y
authors.initials: Y
authors.lastname: Le Fur
authors.forename: David
authors.initials: D
authors.lastname: Manners
authors.forename: Kazuya
authors.initials: K
authors.lastname: Yashiro
authors.forename: Claudia
authors.initials: C
authors.lastname: Testa
authors.forename: Patrick J
authors.initials: PJ
authors.lastname: Cozzone
authors.forename: Stefano
authors.initials: S
authors.lastname: Iotti
authors.forename: David
authors.initials: D
authors.lastname: Bendahan
mesh_terms: D000138:Acidosis / Q000503:physiopathology*; D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000328:Adult; D015444:Exercise / Q000502:physiology*; D005260:Female; D006801:Humans; D006863:Hydrogen-Ion Concentration; D042541:Intracellular Space / Q000378:metabolism*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D018482:Muscle, Skeletal / Q000503:physiopathology*; D010084:Oxidation-Reduction; D010725:Phosphocreatine / Q000378:metabolism*; D010759:Phosphorus Isotopes; D010766:Phosphorylation; D011522:Protons; D012146:Rest / Q000502:physiology
chemical_list: D010759:Phosphorus Isotopes; D011522:Protons; D010725:Phosphocreatine; D000255:Adenosine Triphosphate
keywords: exercise; magnetic resonance spectroscopy; mitochondrial function; muscle acidosis; muscle oxidative capacity; skeletal muscle