title: Clear cell odontogenic carcinomas show EWSR1 rearrangements: a novel finding and a biological link to salivary clear cell carcinomas.
abstract: Clear cell odontogenic carcinomas (CCOCs) are a rare tumor of the jaws, which have considerable morphologic and immunophenotypic overlap with (hyalinizing) clear cell carcinomas (CCCs) of salivary origin. Fluorescence in situ hybridization for EWSR1 was performed on 12 CCOCs, 14 CCCs, and a control set of other miscellaneous clear cell tumors of the head and neck region. EWSR1 was rearranged in 12/13 (92.3%) CCCs and 5/8 (62.5%) CCOCs. EWSR1 testing failed in 1 CCC and 4 CCOCs. Two cases initially diagnosed as CCOCs that were negative for the EWSR1 translocation, were reclassified as clear cell calcifying epithelial odontogenic tumors. ATF1 involvement was confirmed by fluorescence in situ hybridization analysis in 1 CCOC. In this study, we demonstrate for the first time the EWSR1-ATF1 translocation in a CCOC and demonstrate a concrete link between CCCs and at least a subset of CCOCs.
authors.affiliation: School of Dental Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA. elizabeth.bilodeau@dental.pitt.edu
authors.forename: Elizabeth A
authors.initials: EA
authors.lastname: Bilodeau
authors.forename: Ilan
authors.initials: I
authors.lastname: Weinreb
authors.forename: Cristina R
authors.initials: CR
authors.lastname: Antonescu
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.forename: Sanja
authors.initials: S
authors.lastname: Dacic
authors.forename: Susan
authors.initials: S
authors.lastname: Muller
authors.forename: Bruce
authors.initials: B
authors.lastname: Barker
authors.forename: Raja R
authors.initials: RR
authors.lastname: Seethala
mesh_terms: D018262:Adenocarcinoma, Clear Cell / Q000235:genetics* / Q000473:pathology; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002148:Calmodulin-Binding Proteins / Q000235:genetics*; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D015321:Gene Rearrangement; D006258:Head and Neck Neoplasms / Q000235:genetics / Q000473:pathology; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008875:Middle Aged; D009808:Odontogenic Tumors / Q000235:genetics* / Q000473:pathology; D015514:Oncogene Proteins, Fusion / Q000235:genetics; D034802:RNA-Binding Protein EWS; D016601:RNA-Binding Proteins / Q000235:genetics*; D012468:Salivary Gland Neoplasms / Q000235:genetics* / Q000473:pathology; D055815:Young Adult
chemical_list: D002148:Calmodulin-Binding Proteins; D004273:DNA, Neoplasm; C500986:EWSR1 protein, human; C560860:EWSR1-ATF1 fusion protein, human; D015514:Oncogene Proteins, Fusion; D034802:RNA-Binding Protein EWS; D016601:RNA-Binding Proteins