title: Proteomic analysis of acetylation in thermophilic Geobacillus kaustophilus.
abstract: Recent analysis of prokaryotic N(ε)-lysine-acetylated proteins highlights the posttranslational regulation of a broad spectrum of cellular proteins. However, the exact role of acetylation remains unclear due to a lack of acetylated proteome data in prokaryotes. Here, we present the N(ε)-lysine-acetylated proteome of gram-positive thermophilic Geobacillus kaustophilus. Affinity enrichment using acetyl-lysine-specific antibodies followed by LC-MS/MS analysis revealed 253 acetylated peptides representing 114 proteins. These acetylated proteins include not only common orthologs from mesophilic Bacillus counterparts, but also unique G. kaustophilus proteins, indicating that lysine acetylation is pronounced in thermophilic bacteria. These data complement current knowledge of the bacterial acetylproteome and provide an expanded platform for better understanding of the function of acetylation in cellular metabolism.
authors.affiliation: Superbacteria Research Center, Korea Research Institute of Bioscience and Biotechnology-KRIBB, Daejeon, Korea.
authors.forename: Dong-Woo
authors.initials: DW
authors.lastname: Lee
authors.forename: Dooil
authors.initials: D
authors.lastname: Kim
authors.forename: Yong-Jik
authors.initials: YJ
authors.lastname: Lee
authors.forename: Jung-Ae
authors.initials: JA
authors.lastname: Kim
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Choi
authors.forename: Sunghyun
authors.initials: S
authors.lastname: Kang
authors.forename: Jae-Gu
authors.initials: JG
authors.lastname: Pan
mesh_terms: D000107:Acetylation; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000032:analysis* / Q000378:metabolism; D030562:Databases, Protein; D056506:Geobacillus / Q000737:chemistry / Q000378:metabolism*; D053858:Metabolic Networks and Pathways; D008969:Molecular Sequence Data; D020543:Proteome / Q000032:analysis* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*
chemical_list: D001426:Bacterial Proteins; D020543:Proteome
keywords: Acetylation; Geobacillus kaustophilus; Microbiology; Posttranslational modification; Proteome; Thermophile