title: Differential Exoproteome analysis of two corynebacterium pseudotuberculosis biovar ovis strains isolated from goat (1002) and sheep (C231).
abstract: Corynebacterium pseudotuberculosis is the etiologic agent of caseous lymphadenitis a chronic infectious disease affecting small ruminants. The 2D-DIGE technique was used to compare the exoproteomes of two C. pseudotuberculosis biovar ovis strains isolated from goat (strain 1002) and sheep (strain C231). Seventeen proteins differentially produced were identified here. Nine proteins appeared over-produced in the exoproteome of 1002 goat strain and 8 in that of C231 sheep strain. These proteins were related to various biological functions, such as the cell envelope, respiratory metabolism and proteolysis. This proteomic analysis revealed strain-specific exoproteins although each of the corresponding genes was found in both strain genomes. Such differential expression pattern may reflect inter-strain differences in adaptation to a specific host, in pathogenicity and or in antigenicity of this pathogenic bacterium.
authors.affiliation: Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Brazil.
authors.forename: Wanderson M
authors.initials: WM
authors.lastname: Silva
authors.forename: Núbia
authors.initials: N
authors.lastname: Seyffert
authors.forename: Alessandra
authors.initials: A
authors.lastname: Ciprandi
authors.forename: Agenor V
authors.initials: AV
authors.lastname: Santos
authors.forename: Thiago L P
authors.initials: TL
authors.lastname: Castro
authors.forename: Luis G C
authors.initials: LG
authors.lastname: Pacheco
authors.forename: Debmalya
authors.initials: D
authors.lastname: Barh
authors.forename: Yves
authors.initials: Y
authors.lastname: Le Loir
authors.forename: Adriano M C
authors.initials: AM
authors.lastname: Pimenta
authors.forename: Anderson
authors.initials: A
authors.lastname: Miyoshi
authors.forename: Artur
authors.initials: A
authors.lastname: Silva
authors.forename: Vasco
authors.initials: V
authors.lastname: Azevedo
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D003354:Corynebacterium Infections / Q000382:microbiology / Q000662:veterinary*; D016925:Corynebacterium pseudotuberculosis / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D015511:Goat Diseases / Q000382:microbiology*; D006041:Goats; D040901:Proteomics; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins