title: A self-calibrating bipartite viscosity sensor for mitochondria.
abstract: A self-calibrating bipartite viscosity sensor 1 for cellular mitochondria, composed of coumarin and boron-dipyrromethene (BODIPY) with a rigid phenyl spacer and a mitochondria-targeting unit, was synthesized. The sensor showed a direct linear relationship between the fluorescence intensity ratio of BODIPY to coumarin or the fluorescence lifetime ratio and the media viscosity, which allowed us to determine the average mitochondrial viscosity in living HeLa cells as ca. 62 cP (cp). Upon treatment with an ionophore, monensin, or nystatin, the mitochondrial viscosity was observed to increase to ca. 110 cP.
authors.affiliation: Department of Chemistry, Korea University, Seoul 136-701, Korea.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Yang
authors.forename: Yanxia
authors.initials: Y
authors.lastname: He
authors.forename: Jae-Hong
authors.initials: JH
authors.lastname: Lee
authors.forename: Nayoung
authors.initials: N
authors.lastname: Park
authors.forename: Myungkoo
authors.initials: M
authors.lastname: Suh
authors.forename: Weon-Sik
authors.initials: WS
authors.lastname: Chae
authors.forename: Jianfang
authors.initials: J
authors.lastname: Cao
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Peng
authors.forename: Hyosung
authors.initials: H
authors.lastname: Jung
authors.forename: Chulhun
authors.initials: C
authors.lastname: Kang
authors.forename: Jong Seung
authors.initials: JS
authors.lastname: Kim
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D001895:Boron / Q000737:chemistry; D003374:Coumarins / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D008928:Mitochondria / Q000737:chemistry*; D011162:Porphobilinogen / Q000031:analogs & derivatives / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods; D014783:Viscosity
chemical_list: D003374:Coumarins; D005456:Fluorescent Dyes; C507544:dipyrromethene; D011162:Porphobilinogen; C030123:coumarin; D001895:Boron