title: Fabrication of conductive NGF-conjugated polypyrrole-poly(l-lactic acid) fibers and their effect on neurite outgrowth.
abstract: In order to fabricate a tissue scaffold with the neurotrophic and electrical activities, conductive nerve growth factor (NGF)-conjugated polypyrrole-poly(l-lactic acid) (PPy-PLLA) composite fibers were prepared by oxidation polymerization and EDC chemistry with poly-l-lysine. PPy nanoparticles (∼70nm diameter) accumulated on PLLA fiber surface to form a rough thick shell (∼200nm thickness). These NGF-conjugated PPy-PLLA fibers could support PC12 neurite outgrowth and extension. Especially, 40% and 74% increase in PC12 neurite outgrowth and extension, respectively, could be obtained under electrical stimulation of 100mV/cm voltages through the composite fibers. A mechanism for the interaction between neurite extension and the NGF-conjugated PPy-PLLA fibers under electro-stimulation was proposed, to explain the synergistic effect of the rough PPy shell, conjugated NGF and electricity on neurite outgrowth and elongation.
authors.affiliation: College of Materials Science and Engineering, Sichuan University, No. 24, South 1st Section, 1st Ring Road, Chengdu, Sichuan 610065, China.
authors.forename: Jingwen
authors.initials: J
authors.lastname: Zeng
authors.forename: Zhongbing
authors.initials: Z
authors.lastname: Huang
authors.forename: Guangfu
authors.initials: G
authors.lastname: Yin
authors.forename: Jiabang
authors.initials: J
authors.lastname: Qin
authors.forename: Xianchun
authors.initials: X
authors.lastname: Chen
authors.forename: Jianwen
authors.initials: J
authors.lastname: Gu
mesh_terms: D000818:Animals; D004553:Electric Conductivity; D019344:Lactic Acid / Q000737:chemistry / Q000494:pharmacology*; D020932:Nerve Growth Factor / Q000737:chemistry*; D016501:Neurites / Q000187:drug effects* / Q000378:metabolism; D010084:Oxidation-Reduction; D016716:PC12 Cells; D010316:Particle Size; D011091:Polyesters; D058105:Polymerization; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D011758:Pyrroles / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D013499:Surface Properties
chemical_list: D011091:Polyesters; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D019344:Lactic Acid; C033616:poly(lactide); D020932:Nerve Growth Factor