title: Molecular characterization of the leptin receptor gene as a candidate gene in the pulmonary hypertension syndrome in broiler chickens.
abstract: Leptin Receptor Gene (LEPR) is a candidate gene in understanding the genetic basis of the Pulmonary Hypertension Syndrome (PHS) in broilers. Identification and evaluation of genetic polymorphisms in LEPR may provide a link between traits like Body Weight (BW) and Total Ventricle weight (TV) to the development of PHS. In this study, primers were designed in exons, upstream and downstream sequences to identify mutations in the LEPR on four broilers selected with respect to the PHS-related traits. About 77% of the 11,820 bp of the LEPR gene covered by the primers were sequenced. No mutations were found between the chickens associating the traits to the occurrence of PHS. However, 42 single nucleotide polymorphisms and four Indels were found between the reference sequences of the red jungle fowl and the experimental population. Ten of these mutations were not previously reported in LEPR at the genomic and transcript sequences (NP_989654.1, ENSGALT00000018009). The 10 mutations include six SNPs in intron regions, two Indels and two non-synonymous SNPs. The two new non-synonymous SNPs; G301A and A1637G, led to amino acid change A89T and N534S, respectively.
authors.affiliation: Animal Breeding and Genomics Centre, Wageningen University, The Netherlands.
authors.forename: O
authors.initials: O
authors.lastname: Bamidele
authors.forename: P
authors.initials: P
authors.lastname: Van As
authors.forename: M G
authors.initials: MG
authors.lastname: Elferink
mesh_terms: D000818:Animals; D002645:Chickens / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D005091:Exons; D006976:Hypertension, Pulmonary / Q000235:genetics*; D007438:Introns; D008297:Male; D009154:Mutation; D020641:Polymorphism, Single Nucleotide; D054411:Receptors, Leptin / Q000235:genetics*
chemical_list: D017931:DNA Primers; D054411:Receptors, Leptin