title: Protective immunity induced by a DNA vaccine-encoding Toxoplasma gondii microneme protein 11 against acute toxoplasmosis in BALB/c mice.
abstract: Toxoplasma gondii is one of the most prevalent intracellular parasites and is threatening the health of both humans and animals, therefore causing incalculable economic losses worldwide. Vaccination is thought to be an efficient way of controlling toxoplasmosis. T. gondii microneme protein 11 (MIC11) is a soluble microneme protein which is presumably considered facilitating the early stage of cell invasion. To evaluate the protective efficacy of T. gondii MIC11, in the present study, a new DNA vaccine-encoding the α-chain of T. gondii MIC11 was constructed using the pcDNA3.1 vector. Expression of MIC11 from this vector was confirmed by indirect immunofluorescence assay following transfection into baby hamster kidney (BHK) cells. Intramuscular immunization of BALB/c mice with pcDNA/MIC11 was carried out to evaluate the immune responses by serum antibodies titers, lymphoproliferation assay, and cytokines assay. The protective efficacy was evaluated by survival rate in mice after challenging with highly virulent strain of T. gondii. The results demonstrated that this vaccination elicited significant humoral responses and T. gondii lysate antigen (TLA)-stimulated lymphoproliferation (p < 0.05). Compared to controls, the pcDNA/MIC11 immunized mice had high production of IFN-γ, IL-12, and IL-2 (p < 0.05), but not IL-4 (p > 0.05), indicating that a predominant Th1 type response was developed. The vaccination also increased the survival rate of immunized mice when they were challenged with a lethal dose of tachyzoites of T. gondii RH strain. These data suggest that T. gondii MIC11 is a reasonable vaccine candidate deserving further studies, and pcDNA/MIC11 is a potential strategy for the control of toxoplasmosis.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Tao
authors.forename: Rui
authors.initials: R
authors.lastname: Fang
authors.forename: Weichao
authors.initials: W
authors.lastname: Zhang
authors.forename: Yifan
authors.initials: Y
authors.lastname: Wang
authors.forename: Jianxi
authors.initials: J
authors.lastname: Cheng
authors.forename: Yalin
authors.initials: Y
authors.lastname: Li
authors.forename: Kun
authors.initials: K
authors.lastname: Fang
authors.forename: Muhammad Kasib
authors.initials: MK
authors.lastname: Khan
authors.forename: Min
authors.initials: M
authors.lastname: Hu
authors.forename: Yanqin
authors.initials: Y
authors.lastname: Zhou
authors.forename: Junlong
authors.initials: J
authors.lastname: Zhao
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation / Q000276:immunology; D008214:Lymphocytes / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D015800:Protozoan Proteins / Q000276:immunology*; D016052:Protozoan Vaccines / Q000276:immunology*; D011897:Random Allocation; D013154:Spleen / Q000166:cytology; D014122:Toxoplasma / Q000276:immunology / Q000378:metabolism*; D014124:Toxoplasmosis, Animal / Q000276:immunology / Q000517:prevention & control*; D019444:Vaccines, DNA / Q000276:immunology
chemical_list: D016207:Cytokines; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D019444:Vaccines, DNA