title: Flame Aerosol Deposition of TiO2Nanoparticle Films on Polymers and Polymeric Microfluidic Devices for On-Chip Phosphopeptide Enrichment.
abstract: Direct and fast (10s of seconds) deposition of flame-made, high surface-area aerosol films on polymers and polymeric microfluidic devices is demonstrated. Uniform TiO2nanoparticle films were deposited on cooled Poly(methyl methacrylate) (PMMA) substrates by combustion of titanium(IV) isopropoxide (TTIP) - xylene solution sprays. Films were mechanically stabilized by in-situ annealing with a xylene spray flame. Plasma-etched microfluidic chromatography columns, comprising parallel microchannels were also coated with such nanoparticle films without any microchannel deformation. These microcolumns were successfully used in metal-oxide affinity chromatography (MOAC) to selectively trap phosphopeptides on these high surface-area nanostructured films. The chips had a high capacity retaining 1.2 μg of standard phosphopeptide. A new extremely fast method is developed for MOAC microchip stationary phase fabrication with applications in proteomics.
authors.affiliation: Particle Technology Laboratory, Institute of Process Engineering, Department of Mechanical and Process Engineering, ETH Zurich 8092 Zurich, Switzerland.
authors.forename: Thomas
authors.initials: T
authors.lastname: Rudin
authors.forename: Katerina
authors.initials: K
authors.lastname: Tsougeni
authors.forename: Evangelos
authors.initials: E
authors.lastname: Gogolides
authors.forename: Sotiris E
authors.initials: SE
authors.lastname: Pratsinis