title: Effects of Gaps Induced Into the ACL Tendon Graft on Tendon-Bone Healing in a Rodent ACL Reconstruction Model.
abstract: Graft necrosis following ACL reconstruction is often associated with the use of autologous grafts. Host cells rather than graft cells contribute to the repair of the tendon-bone interface and the remodeling of the autologous graft. The native tendon-bone interface is not recreated and the biomechanical properties are not restored back to native values. We examined the effects of introducing gaps within the tendon graft prior to ACL reconstruction in a rodent model. We hypothesised that gaps will make physical way for host cells to infiltrate and repopulate the graft and thus enhance healing. Animals were sacrificed at seven, fourteen, and twenty-eight days for biomechanical testing and histology. Our findings indicate that graft necrosis, usually observed in the initial two weeks of the healing process, is averted. Histological observations showed that tendon-bone healing stages were hastened however this didn't translate into improved biomechanical properties.
authors.affiliation: Surgical and Orthopaedic Research Laboratories, University of New South Wales, Prince of Wales Hospital, Sydney, Australia.
authors.forename: Vedran
authors.initials: V
authors.lastname: Lovric
authors.forename: Tomonoshin
authors.initials: T
authors.lastname: Kanazawa
authors.forename: Yoshinari
authors.initials: Y
authors.lastname: Nakamura
authors.forename: Rema A
authors.initials: RA
authors.lastname: Oliver
authors.forename: Yan
authors.initials: Y
authors.lastname: Yu
authors.forename: William Robert
authors.initials: WR
authors.lastname: Walsh
keywords: ACL reconstruction; graft cells; graft necrosis; host cells; rodent model; tendon-bone healing