title: Detection of the inflammatory process in a Behçet's disease-like mouse model using 18F-fluorodeoxyglucose positron emission tomography.
abstract: OBJECTIVE
  The major role of herpes simplex virus (HSV) type 1 infection in Behçet's disease (BD) immunopathogenesis has been demonstrated and inoculating the earlobes of ICR mice with HSV produced a BD-like mouse model. (18)Ffluorodeoxyglucose positron emission tomography (FDG PET) is widely used for diagnosing numerous human diseases other than malignancies. The aim of our study was to evaluate the inflammatory activities of BD-like symptoms in a HSV type 1-induced BD-like mouse model by small-animal FDG PET.
  
  
  METHODS
  Five HSV-infected ICR mice with BD-like lesions, two asymptomatic HSV-infected mice, and two untreated mice were scanned with microPET, and autopsy specimens were histopathologically assessed to evaluate for infiltration by mixed inflammatory cells.
  
  
  RESULTS
  The histopathological evaluation of the inflammatory process in knee and elbow joints significantly correlated with the quantitative assessment of FDG accumulation in the same joints in BD-like ICR mice, HSV-infected asymptomatic mice, and untreated control mice. Small-animal FDG PET clearly detected asymptomatic joint inflammatory processes in both BD-like mice and HSV-infected asymptomatic mice. In addition, genital ulcers and skin ulcers with associated perilesional lymphadenopathies in BD-like models were detected by microPET. However, biodistributed PET-positive images from the stasis of secreted FDG into the bowel lumen could not be distinguished from the inflammatory bowel lesions of BD when compared to FDG uptake in control mice.
  
  
  CONCLUSIONS
  Our data indicate that FDG PET can non-invasively and quantitatively detect the inflammatory process in an HSV-induced BD-like mouse model.
authors.affiliation: Department of Dermatology and Cutaneous Biology Research Institute, Yonsei University College of Medicine, Seoul, Korea. sbcho@yuhs.ac.
authors.forename: Sung Bin
authors.initials: SB
authors.lastname: Cho
authors.forename: Seonghyang
authors.initials: S
authors.lastname: Sohn
authors.forename: Zhenlong
authors.initials: Z
authors.lastname: Zheng
authors.forename: Arthur
authors.initials: A
authors.lastname: Cho
authors.forename: Hyunki
authors.initials: H
authors.lastname: Kim
authors.forename: Won Jun
authors.initials: WJ
authors.lastname: Kang
authors.forename: Dongsik
authors.initials: D
authors.lastname: Bang
authors.forename: Mijin
authors.initials: M
authors.lastname: Yun
authors.forename: Jong Doo
authors.initials: JD
authors.lastname: Lee
authors.forename: Jae-Hoon
authors.initials: JH
authors.lastname: Lee
mesh_terms: D000818:Animals; D001528:Behcet Syndrome / Q000000981:diagnostic imaging* / Q000276:immunology / Q000473:pathology / Q000821:virology; D004195:Disease Models, Animal; D019788:Fluorodeoxyglucose F18; D006561:Herpes Simplex / Q000000981:diagnostic imaging* / Q000276:immunology / Q000473:pathology / Q000821:virology; D018259:Herpesvirus 1, Human / Q000276:immunology / Q000472:pathogenicity; D007249:Inflammation / Q000000981:diagnostic imaging* / Q000276:immunology / Q000473:pathology / Q000821:virology; D007596:Joints / Q000000981:diagnostic imaging* / Q000276:immunology / Q000473:pathology / Q000821:virology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D049268:Positron-Emission Tomography; D011237:Predictive Value of Tests; D019275:Radiopharmaceuticals
chemical_list: D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18