title: Effect of sleep deprivation on rhythms of clock gene expression and melatonin in humans.
abstract: This study investigated the impact of sleep deprivation on the human circadian system. Plasma melatonin and cortisol levels and leukocyte expression levels of 12 genes were examined over 48 h (sleep vs. no-sleep nights) in 12 young males (mean±SD: 23±5 yrs). During one night of total sleep deprivation, BMAL1 expression was suppressed, the heat shock gene HSPA1B expression was induced, and the amplitude of the melatonin rhythm increased, whereas other high-amplitude clock gene rhythms (e.g., PER1-3, REV-ERBα) remained unaffected. These data suggest that the core clock mechanism in peripheral oscillators is compromised during acute sleep deprivation.
authors.affiliation: Department of Forensic Molecular Biology, Erasmus MC University Medical Center Rotterdam, Rotterdam, The Netherlands. ka44@st-andrews.ac.uk
authors.forename: Katrin
authors.initials: K
authors.lastname: Ackermann
authors.forename: Rosina
authors.initials: R
authors.lastname: Plomp
authors.forename: Oscar
authors.initials: O
authors.lastname: Lao
authors.forename: Benita
authors.initials: B
authors.lastname: Middleton
authors.forename: Victoria L
authors.initials: VL
authors.lastname: Revell
authors.forename: Debra J
authors.initials: DJ
authors.lastname: Skene
authors.forename: Manfred
authors.initials: M
authors.lastname: Kayser
mesh_terms: D056930:ARNTL Transcription Factors / Q000378:metabolism; D000328:Adult; D002940:Circadian Rhythm / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D007962:Leukocytes / Q000378:metabolism; D008027:Light; D008297:Male; D008550:Melatonin / Q000097:blood*; D056953:Nuclear Receptor Subfamily 1, Group D, Member 1 / Q000235:genetics / Q000378:metabolism; D009991:Oscillometry / Q000379:methods; D018384:Oxidative Stress; D056950:Period Circadian Proteins / Q000235:genetics / Q000378:metabolism*; D011863:Radioimmunoassay; D012890:Sleep / Q000235:genetics; D012892:Sleep Deprivation / Q000235:genetics* / Q000378:metabolism*; D013997:Time Factors; D055815:Young Adult
chemical_list: D056930:ARNTL Transcription Factors; C538960:BMAL1 protein, human; D018840:HSP70 Heat-Shock Proteins; C525621:HSPA1B protein, human; C064353:NR1D1 protein, human; D056953:Nuclear Receptor Subfamily 1, Group D, Member 1; C539022:PER1 protein, human; C421902:PER2 protein, human; C512644:PER3 protein, human; D056950:Period Circadian Proteins; D008550:Melatonin; D006854:Hydrocortisone