title: Whole-rat conditional gene knockout via genome editing.
abstract: Animal models with genetic modifications under temporal and/or spatial control are invaluable to functional genomics and medical research. Here we report the generation of tissue-specific knockout rats via microinjection of zinc-finger nucleases (ZFNs) into fertilized eggs. We generated rats with loxP-flanked (floxed) alleles and a tyrosine hydroxylase promoter-driven cre allele and demonstrated Cre-dependent gene disruption in vivo. Pronuclear microinjection of ZFNs, shown by our data to be an efficient and rapid method for creating conditional knockout rats, should also be applicable in other species.
authors.affiliation: Sage Labs, St. Louis, Missouri, USA.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Brown
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Fisher
authors.forename: Evguenia
authors.initials: E
authors.lastname: Kouranova
authors.forename: Aaron
authors.initials: A
authors.lastname: McCoy
authors.forename: Kevin
authors.initials: K
authors.lastname: Forbes
authors.forename: Yumei
authors.initials: Y
authors.lastname: Wu
authors.forename: Rachel
authors.initials: R
authors.lastname: Henry
authors.forename: Diana
authors.initials: D
authors.lastname: Ji
authors.forename: Andre
authors.initials: A
authors.lastname: Chambers
authors.forename: Joe
authors.initials: J
authors.lastname: Warren
authors.forename: Weiguo
authors.initials: W
authors.lastname: Shu
authors.forename: Edward J
authors.initials: EJ
authors.lastname: Weinstein
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Cui
mesh_terms: D000818:Animals; D003851:Deoxyribonucleases / Q000235:genetics*; D055786:Gene Knockout Techniques / Q000379:methods*; D005818:Genetic Engineering / Q000379:methods; D016678:Genome / Q000235:genetics*; D051381:Rats / Q000196:embryology* / Q000235:genetics*; D055647:Rats, Transgenic; D014162:Transfection / Q000379:methods*; D016335:Zinc Fingers / Q000235:genetics*
chemical_list: D003851:Deoxyribonucleases