title: Evaluation of metal ion release from Ti6Al4V and Co-Cr-Mo casting alloys: in vivo and in vitro study.
abstract: OBJECTIVE
  The aim of this study was to evaluate the amount of ions released from Ti6Al4V and Co-Cr-Mo alloys both in vivo and in vitro.
  
  
  METHODS
  Twenty-one discs of each alloy were constructed and divided into seven groups. Three specimens from each group were immersed in a buffered saline solution over a period of 1, 3, 5, 7, 14, 21, and 28 days. Twenty-eight participants were also included in the study, where the study group consisted of 14 mandibular partially edentulous patients, and the control group consisted of 14 volunteers. The study group was further divided into two equal groups: the first group received removable partial dentures (RPDs) constructed from Co-Cr-Mo alloy, while the second group received RPDs constructed from Ti6Al4V alloy. Saliva samples were collected from each participant over the same study period. The conditioning media and saliva samples were analyzed using a spectrophotometer. One-way ANOVA and Tukey tests were used for statistical analysis (p < 0.05).
  
  
  RESULTS
  The concentrations of metal ions released from the studied alloys were significantly higher in the in vitro than in the in vivo study group during the follow-up periods. A statistically significant increase in ion concentrations of the different elements for both alloys was found with time (p < 0.05).
  
  
  CONCLUSIONS
  The amounts of released metallic ions from Co-Cr-Mo and Ti6Al4V alloys were higher in the buffered saline solutions than in the studied saliva samples and control groups; however, these amounts were still within the physiological limit of trace elements in the human body.
authors.affiliation: Associate Professor of Prosthodontics, Faculty of Dentistry, Minia University, Minia, Egypt.
authors.forename: Amal A
authors.initials: AA
authors.lastname: El Sawy
authors.forename: Mohammed A
authors.initials: MA
authors.lastname: Shaarawy
mesh_terms: D000328:Adult; D000497:Alloys; D000535:Aluminum / Q000032:analysis; D002857:Chromium / Q000032:analysis; D002858:Chromium Alloys / Q000737:chemistry*; D003035:Cobalt / Q000032:analysis; D017077:Culture Media, Conditioned; D003722:Dental Alloys / Q000737:chemistry*; D003779:Denture Design; D003832:Denture, Partial, Removable; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007101:Immersion; D066298:In Vitro Techniques; D007576:Jaw, Edentulous, Partially / Q000534:rehabilitation; D008297:Male; D008422:Materials Testing; D008670:Metals / Q000737:chemistry*; D008875:Middle Aged; D008982:Molybdenum / Q000032:analysis; D012463:Saliva / Q000737:chemistry; D012965:Sodium Chloride / Q000737:chemistry; D013053:Spectrophotometry; D013997:Time Factors; D014025:Titanium / Q000032:analysis / Q000737:chemistry*
chemical_list: D000497:Alloys; D002858:Chromium Alloys; D017077:Culture Media, Conditioned; D003722:Dental Alloys; D008670:Metals; D002857:Chromium; C031462:titanium alloy (TiAl6V4); D003035:Cobalt; D012965:Sodium Chloride; D008982:Molybdenum; D000535:Aluminum; D014025:Titanium
keywords: Co-Cr-Mo alloy; Ti6Al4V alloy; metal ion concentration; metal ion release; removable partial dentures