title: PCR-based diagnosis of prosthetic joint infection.
abstract: We performed a meta-analysis to evaluate use of PCR assays for diagnosis of prosthetic joint infection (PJI). The pooled sensitivity and specificity were 0.86 (95% confidence interval [CI], 0.77 to 0.92) and 0.91 (CI, 0.81 to 0.96), respectively. Subgroup analyses showed that use of tissue samples may improve sensitivity, and quantitative PCR and sonication of prostheses fluid may improve specificity. The results showed that PCR is reliable and accurate for detection of PJI.
authors.affiliation: Department of Orthopedics, Shanghai Key Laboratory of Orthopedic Implants, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Qu
authors.forename: Zanjing
authors.initials: Z
authors.lastname: Zhai
authors.forename: Huiwu
authors.initials: H
authors.lastname: Li
authors.forename: Haowei
authors.initials: H
authors.lastname: Li
authors.forename: Xuqiang
authors.initials: X
authors.lastname: Liu
authors.forename: Zhenan
authors.initials: Z
authors.lastname: Zhu
authors.forename: You
authors.initials: Y
authors.lastname: Wang
authors.forename: Guangwang
authors.initials: G
authors.lastname: Liu
authors.forename: Kerong
authors.initials: K
authors.lastname: Dai
mesh_terms: D006801:Humans; D008828:Microbiological Techniques / Q000379:methods*; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D010003:Osteoarthritis / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods*; D016459:Prosthesis-Related Infections / Q000175:diagnosis*; D012680:Sensitivity and Specificity