title: Influenza vaccine: development of a novel intranasal and subcutaneous recombinant adjuvant.
abstract: The synthetic peptide GK-1, derived from Taenia crassiceps, enhances the protection induced by human influenza vaccine in both young and aged mice. Herein, the adjuvant properties of GK-1 fused to the pVIII protein of a heat-inactivated phagemid vector (FGK1) when co-administered with the influenza vaccine were assessed, to evaluate its feasibility as a low-cost adjuvant. In mice, FGK1 significantly increased the expected IgG and IgA anti-influenza antibody levels both in sera and in bronchoalveolar fluids when intranasally or subcutaneously co-administered with influenza vaccine. Single-dose pig co-immunization with FGK1 and influenza vaccine induced serum levels of IgG anti-influenza antibodies similar to those elicited by a two-dose immunization with the influenza vaccine alone. Preclinical evaluation of FGK1 with the influenza vaccine is currently in progress, in order to recommend its use for veterinary purposes.
authors.affiliation: Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México 04510, D.F., Mexico.
authors.forename: R
authors.initials: R
authors.lastname: Segura-Velázquez
authors.forename: J
authors.initials: J
authors.lastname: Cervantes
authors.forename: E
authors.initials: E
authors.lastname: Acosta
authors.forename: I
authors.initials: I
authors.lastname: Sánchez-Betancourt
authors.forename: N
authors.initials: N
authors.lastname: Villalobos
authors.forename: L F
authors.initials: LF
authors.lastname: Rodarte
authors.forename: M
authors.initials: M
authors.lastname: Restelli
authors.forename: G
authors.initials: G
authors.lastname: Fragoso
authors.forename: E
authors.initials: E
authors.lastname: Sciutto
mesh_terms: D000276:Adjuvants, Immunologic / Q000235:genetics* / Q000494:pharmacology; D000281:Administration, Intranasal; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D017104:Bacteriophage M13 / Q000235:genetics; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D005260:Female; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007252:Influenza Vaccines / Q000008:administration & dosage* / Q000276:immunology* / Q000494:pharmacology; D007263:Infusions, Parenteral; D007279:Injections, Subcutaneous; D008423:Maternal Age; D051379:Mice; D008807:Mice, Inbred BALB C; D010455:Peptides / Q000276:immunology; D011136:Polysorbates / Q000008:administration & dosage / Q000494:pharmacology; D013185:Squalene / Q000008:administration & dosage / Q000494:pharmacology; D013552:Swine; D013621:Taenia; D014611:Vaccination / Q000379:methods
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007252:Influenza Vaccines; C089950:MF59 oil emulsion; D010455:Peptides; D011136:Polysorbates; D013185:Squalene
keywords: Adjuvant; Influenza; Intranasal immunization; Phages; Vaccination