title: Application of the single blood sample method to estimate feline glomerular filtration rate in a clinically relevant situation.
abstract: To compare glomerular filtration rate (GFR) estimated by a single blood sample method, the non-ionic contrast medium iodixanol (40 mg I/kg) and the standard GFR tracer inulin (50 mg/kg) were co-administered as a bolus intravenous injection to 12 cats, followed by blood collection 60 and 90 mins later. Serum iodixanol and inulin concentrations were measured separately by high-performance liquid chromatography and colourimetric assay. A correlation (r = 0.90, P <0.01) was noted between GFR values estimated by the single-blood-sample method using iodixanol and inulin, indicating that this procedure can apply to feline GFR estimates, even if different GFR tracers are used. In a feline kidney transplantation study, the GFR was monitored subsequently by this simplified iodixanol method throughout a 750-day observation period with no adverse reactions. The results demonstrate that the simplified method, including the volume of distribution, can be used as an alternative or expedient tool in a clinically relevant situation.
authors.affiliation: 1Cooperative Department of Veterinary Clinical Medicine, Iwate University, Iwate, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Katayama
authors.forename: Asuka
authors.initials: A
authors.lastname: Sasaki
authors.forename: Mariko
authors.initials: M
authors.lastname: Takayasu
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Shimamura
authors.forename: Yuji
authors.initials: Y
authors.lastname: Uzuka
authors.forename: Isao
authors.initials: I
authors.lastname: Murayama
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Satoh
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Furuhama
mesh_terms: D000818:Animals; D001806:Blood Urea Nitrogen; D002371:Cat Diseases / Q000097:blood* / Q000175:diagnosis; D002415:Cats; D003404:Creatinine; D005919:Glomerular Filtration Rate / Q000662:veterinary*; D007668:Kidney / Q000502:physiology*; D016030:Kidney Transplantation / Q000662:veterinary; D051436:Renal Insufficiency, Chronic / Q000097:blood / Q000175:diagnosis / Q000662:veterinary*
chemical_list: D003404:Creatinine