title: Digital gene expression analysis of corky split vein caused by boron deficiency in 'Newhall' Navel Orange (Citrus sinensis Osbeck) for selecting differentially expressed genes related to vascular hypertrophy.
abstract: Corky split vein caused by boron (B) deficiency in 'Newhall' Navel Orange was studied in the present research. The boron-deficient citrus exhibited a symptom of corky split vein in mature leaves. Morphologic and anatomical surveys at four representative phases of corky split veins showed that the symptom was the result of vascular hypertrophy. Digital gene expression (DGE) analysis was performed based on the Illumina HiSeq™ 2000 platform, which was applied to analyze the gene expression profilings of corky split veins at four morphologic phases. Over 5.3 million clean reads per library were successfully mapped to the reference database and more than 22897 mapped genes per library were simultaneously obtained. Analysis of the differentially expressed genes (DEGs) revealed that the expressions of genes associated with cytokinin signal transduction, cell division, vascular development, lignin biosynthesis and photosynthesis in corky split veins were all affected. The expressions of WOL and ARR12 involved in the cytokinin signal transduction pathway were up-regulated at 1(st) phase of corky split vein development. Furthermore, the expressions of some cell cycle genes, CYCs and CDKB, and vascular development genes, WOX4 and VND7, were up-regulated at the following 2(nd) and 3(rd) phases. These findings indicated that the cytokinin signal transduction pathway may play a role in initiating symptom observed in our study.
authors.affiliation: College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Key Laboratory of Horticultural Plant Biology, Ministry of Education, Wuhan, China. chqyang@webmail.hzau.edu.cn
authors.forename: Cheng-Quan
authors.initials: CQ
authors.lastname: Yang
authors.forename: Yong-Zhong
authors.initials: YZ
authors.lastname: Liu
authors.forename: Ji-Cui
authors.initials: JC
authors.lastname: An
authors.forename: Shuang
authors.initials: S
authors.lastname: Li
authors.forename: Long-Fei
authors.initials: LF
authors.lastname: Jin
authors.forename: Gao-Feng
authors.initials: GF
authors.lastname: Zhou
authors.forename: Qing-Jiang
authors.initials: QJ
authors.lastname: Wei
authors.forename: Hui-Qing
authors.initials: HQ
authors.lastname: Yan
authors.forename: Nan-Nan
authors.initials: NN
authors.lastname: Wang
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Fu
authors.forename: Xiao
authors.initials: X
authors.lastname: Liu
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Hu
authors.forename: Ting-Shuai
authors.initials: TS
authors.lastname: Yan
authors.forename: Shu-Ang
authors.initials: SA
authors.lastname: Peng
mesh_terms: D001895:Boron / Q000172:deficiency*; D032084:Citrus sinensis / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D063990:Gene Ontology; D017343:Genes, Plant; D053858:Metabolic Networks and Pathways / Q000235:genetics; D010935:Plant Diseases; D058526:Plant Vascular Bundle / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D060888:Real-Time Polymerase Chain Reaction; D059467:Transcriptome
chemical_list: D001895:Boron