title: Association between vitamin B12 level and anti-parietal cells and anti-intrinsic factor antibodies among adult Jordanian patients with Helicobacter pylori infection.
abstract: OBJECTIVE
  Evaluate the association of Helicobacter pylori infection with anti-parietal cell antibodies (APCA) and anti-intrinsic factor antibodies (AIFA) and their impact on vitamin B12 serum level.
  
  
  METHODS
  One hundred patients (M/F: 43/57; age 46.5±17.5 years) who underwent upper gastrointestinal endoscopy at King Abdullah University Hospital, Irbid, Jordan were enrolled in the study. The patients were grouped as H. pylori-infected (n=81) or H. pylori negative (n=19) by histopathological examination. Fasting serum vitamin B12 levels, anti-parietal cell antibodies and anti-intrinsic factor antibodies for patients and controls were determined.
  
  
  RESULTS
  Anti-parietal cell antibodies and anti-intrinsic factor antibodies were positive in 9.9% and 18.5% of H. pylori-positive patients respectively. None of the H. pylori negative subjects had anti-parietal cell antibodies or anti-intrinsic factor antibodies. Serum vitamin B12 level was lower in the H. pylori-infected patients (275±70.4pg/mL) than in controls (322.9±60.7pg/mL; p<0.05). H. pylori was positive in 94% of the low-vitamin B12 group compared with 64.6% of the normal-vitamin B12 group (p<0.5).
  
  
  CONCLUSIONS
  Patients with H. pylori infection are more likely to have anti-parietal cell antibodies and anti-intrinsic factor antibodies. There was an association between H. pylori infection and lower vitamin B12 levels. H. pylori infection might be a significant factor in the pathogenesis of autoimmune gastritis.
authors.affiliation: Department of Internal Medicine, Faculty of Medicine, Jordan University of Science and Technology, Irbid, Jordan. Electronic address: ayeshmahmoud@hotmail.com.
authors.forename: Mahmoud H
authors.initials: MH
authors.lastname: Ayesh
authors.forename: Khaled
authors.initials: K
authors.lastname: Jadalah
authors.forename: Eiman
authors.initials: E
authors.lastname: Al Awadi
authors.forename: Khaldoon
authors.initials: K
authors.lastname: Alawneh
authors.forename: Basheer
authors.initials: B
authors.lastname: Khassawneh
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001323:Autoantibodies / Q000097:blood*; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D005757:Gastritis, Atrophic / Q000097:blood / Q000276:immunology* / Q000469:parasitology; D016481:Helicobacter Infections / Q000097:blood / Q000276:immunology* / Q000473:pathology; D016480:Helicobacter pylori; D006801:Humans; D007437:Intrinsic Factor / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D010295:Parietal Cells, Gastric / Q000276:immunology*; D014805:Vitamin B 12 / Q000097:blood*; D055815:Young Adult
chemical_list: D001323:Autoantibodies; D007437:Intrinsic Factor; D014805:Vitamin B 12
keywords: Anti-intrinsic factor antibodies; Anti-parietal cell antibodies; H. pylori; Vitamin B12