title: Peroxynitrite activity of hemin-functionalized reduced graphene oxide.
abstract: Conducting interfaces modified with reduced graphene oxide (rGO) have shown improved electrochemical response for different analytes. The efficient formation of functionalized rGO based materials is thus of current interest for the development of sensitive and selective biosensors. Herein, we report a simple and environmentally friendly method for the formation of a hemin-functionalized rGO hybrid nanomaterial that exhibits remarkable sensitivity to peroxynitrite (ONOO(-)) in solution. The hemin-functionalized rGO hybrid nanomaterial was formed by mixing an aqueous solution of graphene oxide (GO) with hemin and sonicating the suspension for 5 h at room temperature. In addition to playing a key role in biochemical and electrocatalytic reactions, hemin has been proven to be a good reducing agent for GO. The sensitivity of the peroxynitrite sensor is ≈7.5 ± 1.5 nA mM(-1) with a detection limit of 5 ± 1.5 nM.
authors.affiliation: Institut de Recherche Interdisciplinaire (IRI, USR 3078), Université Lille1, Parc de la Haute Borne, 50 Avenue de Halley, BP 70478, 59658 Villeneuve d'Ascq, France.
authors.forename: Raluca
authors.initials: R
authors.lastname: Oprea
authors.forename: Serban F
authors.initials: SF
authors.lastname: Peteu
authors.forename: Palaniappan
authors.initials: P
authors.lastname: Subramanian
authors.forename: Wang
authors.initials: W
authors.lastname: Qi
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Pichonat
authors.forename: Henri
authors.initials: H
authors.lastname: Happy
authors.forename: Mekki
authors.initials: M
authors.lastname: Bayachou
authors.forename: Rabah
authors.initials: R
authors.lastname: Boukherroub
authors.forename: Sabine
authors.initials: S
authors.lastname: Szunerits
mesh_terms: D006108:Graphite / Q000737:chemistry*; D006427:Hemin / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*; D030421:Peroxynitrous Acid / Q000737:chemistry*; D056951:Photoelectron Spectroscopy / Q000379:methods
chemical_list: D010087:Oxides; D030421:Peroxynitrous Acid; D006427:Hemin; D006108:Graphite