title: Exogenous Reelin modifies the migratory behavior of neurons depending on cortical location.
abstract: Malformations of cortical development can arise when projection neurons generated in the germinal zones fail to migrate properly into the cortical plate. This process is critically dependent on the Reelin glycoprotein, which when absent leads to an inversion of cortical layers and blurring of borders. Reelin has other functions including supporting neuron migration and maintaining their trajectories; however, the precise role on glial fiber-dependent or -independent migration of neurons remains controversial. In this study, we wish to test the hypothesis that migrating cortical neurons at different levels of the cortical wall have differential responses to Reelin. We exposed neurons migrating across the cortical wall to exogenous Reelin and monitored their migratory behavior using time-lapse imaging. Our results show that, in the germinal zones, exogenous Reelin retarded neuron migration and altered their trajectories. This behavior is in contrast to the response of neurons located in the intermediate zone (IZ), possibly because Reelin receptors are not expressed in this zone. In the reeler cortex, Reelin receptors are expressed in the IZ and exposure to exogenous Reelin was able to rescue the migratory defect. These studies demonstrate that migrating neurons have nonequivalent responses to Reelin depending on their location within the cortical wall.
authors.affiliation: Brain Development and Regeneration Division, The Florey Institute of Neuroscience and Mental Health, Centre for Neuroscience, University of Melbourne, Parkville, Victoria, Australia and.
authors.forename: Joanne M
authors.initials: JM
authors.lastname: Britto
authors.affiliation: Brain Development and Regeneration Division, The Florey Institute of Neuroscience and Mental Health.
authors.forename: Karen J
authors.initials: KJ
authors.lastname: Tait
authors.affiliation: Brain Development and Regeneration Division, The Florey Institute of Neuroscience and Mental Health.
authors.forename: Ean Phing
authors.initials: EP
authors.lastname: Lee
authors.affiliation: Brain Development and Regeneration Division, The Florey Institute of Neuroscience and Mental Health.
authors.forename: Robin S
authors.initials: RS
authors.lastname: Gamble
authors.affiliation: Department of Biomedical Science, Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, Japan.
authors.forename: Mitsuharu
authors.initials: M
authors.lastname: Hattori
authors.affiliation: Brain Development and Regeneration Division, The Florey Institute of Neuroscience and Mental Health, Centre for Neuroscience, University of Melbourne, Parkville, Victoria, Australia and.
authors.forename: Seong-Seng
authors.initials: SS
authors.lastname: Tan
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000172:deficiency / Q000235:genetics; D000367:Age Factors; D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D015816:Cell Adhesion Molecules, Neuronal / Q000494:pharmacology*; D002461:Cell Line, Transformed; D002465:Cell Movement / Q000187:drug effects* / Q000235:genetics; D002540:Cerebral Cortex / Q000166:cytology*; D018274:Electroporation; D004622:Embryo, Mammalian; D016326:Extracellular Matrix Proteins / Q000494:pharmacology*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D066298:In Vitro Techniques; D051379:Mice; D008818:Mice, Neurologic Mutants; D018613:Microscopy, Confocal; D009419:Nerve Tissue Proteins / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D009924:Organ Culture Techniques; D000091042:Reelin Protein; D012697:Serine Endopeptidases / Q000494:pharmacology*; D014162:Transfection
chemical_list: D033942:Adaptor Proteins, Vesicular Transport; D015816:Cell Adhesion Molecules, Neuronal; D016326:Extracellular Matrix Proteins; D009419:Nerve Tissue Proteins; D000091042:Reelin Protein; D049452:Green Fluorescent Proteins; D000469:Alkaline Phosphatase; C000717128:Reln protein, mouse; D012697:Serine Endopeptidases
keywords: Reelin; bipolar; cortex; migration; multipolar