title: Development of 10 novel polymorphic microsatellite markers for the Indo-Pacific horned starfish, Protoreaster nodosus.
abstract: Ten novel polymorphic nuclear microsatellite simple sequence repeat (SSR) markers were isolated from an Indo-Pacific horned starfish, Protoreaster nodosus. The isolated SSR markers provided polymorphisms of 2-9 alleles per locus in three populations obtained from Indonesia and the Philippines. The expected and observed heterozygosities ranged from 0.049 to 0.691 and from 0.050 to 0.800, respectively. Pairwise FST values among three populations ranged from 0.018 to 0.050 (global FST=0.031). All P. nodosus individuals collected from three populations exhibited different genotypes for the ten identified SSR markers, indicating that P. nodosus reproduces sexually. Then the developed SSR markers will be useful for studying the population and conservation genetics of P. nodosus.
authors.affiliation: Asian Natural Environmental Science Center, The University of Tokyo, Midori-cho, Nishitokyo, Tokyo 188-0002, Japan.
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Nakajima
authors.forename: Nina
authors.initials: N
authors.lastname: Yasuda
authors.forename: Yu
authors.initials: Y
authors.lastname: Matsuki
authors.forename: Dan M
authors.initials: DM
authors.lastname: Arriesgado
authors.forename: Chunlan
authors.initials: C
authors.lastname: Lian
authors.forename: Miguel D
authors.initials: MD
authors.lastname: Fortes
authors.forename: Wilfredo H
authors.initials: WH
authors.lastname: Uy
authors.forename: Wilfredo L
authors.initials: WL
authors.lastname: Campos
authors.forename: Masahiro
authors.initials: M
authors.lastname: Nakaoka
authors.forename: Coralie
authors.initials: C
authors.lastname: Taquet
authors.lastname: Suharsono
authors.forename: Satoshi
authors.initials: S
authors.lastname: Nagai
authors.forename: Kazuo
authors.initials: K
authors.lastname: Nadaoka
mesh_terms: D000818:Animals; D005828:Genetics, Population; D006579:Heterozygote; D007214:Indonesia; D018895:Microsatellite Repeats; D010679:Philippines; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D013215:Starfish / Q000235:genetics*
keywords: Conservation genetics; Larval dispersal; Microsatellite; SSR; Seagrass beds