title: Molecular characterization of unique intersubtype HIV type 1 A1/C recombinant strain circulating in Pune, India.
abstract: An increasing number of circulating recombinant forms (CRFs) and unique recombinant forms (URFs) all over the world has necessitated being vigilant about new recombinants. Since the first report of a recombinant virus with an A1/C mosaic in 1998 more and more B/C and A/C recombinant viruses are being reported from India. Here we report the identification and characterization of a unique HIV-1 A1/C recombinant circulating in Western India. Analysis of the full-length genome using RIP, SimPlot, and jpHMM@Gobics has confirmed its mosaic structure with insertion of subtype A1 in the backbone of subtype C at three positions: gag-pol (1973±15-2617±47), pol-vif (4879±37-5582±32), and gp41 (8437±106-8811±8); however, RIP and SimPlot showed one more small insertion in integrase (4343-4519). Phylogenetic analysis confirmed that the recombinant virus has an insertion of clade A1 in the backbone of subtype C, which has come from Indian subtype C.
authors.affiliation: Division of Immunology, National AIDS Research Institute (ICMR), Pune, India.
authors.forename: Sudhanshu
authors.initials: S
authors.lastname: Pandey
authors.forename: Srikanth
authors.initials: S
authors.lastname: Tripathy
authors.forename: Ramesh
authors.initials: R
authors.lastname: Paranjape
mesh_terms: D000328:Adult; D001483:Base Sequence; D016679:Genome, Viral; D015700:HIV Envelope Protein gp41 / Q000235:genetics*; D015658:HIV Infections / Q000453:epidemiology* / Q000821:virology*; D019427:HIV Integrase / Q000235:genetics; D015497:HIV-1 / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D007194:India; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D017423:Sequence Analysis, RNA; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000235:genetics*; D054302:pol Gene Products, Human Immunodeficiency Virus / Q000235:genetics*; D054320:vif Gene Products, Human Immunodeficiency Virus / Q000235:genetics*
chemical_list: D015700:HIV Envelope Protein gp41; D012367:RNA, Viral; D054301:gag Gene Products, Human Immunodeficiency Virus; C514787:gp41 protein, Human immunodeficiency virus 1; D054302:pol Gene Products, Human Immunodeficiency Virus; D054320:vif Gene Products, Human Immunodeficiency Virus; D019427:HIV Integrase