title: Single-walled carbon nanotube-mediated small interfering RNA delivery for gastrin-releasing peptide receptor silencing in human neuroblastoma.
abstract: Small interfering RNA (siRNA) has the potential to influence gene expression with a high degree of target gene specificity. However, the clinical application of siRNA therapeutics has proven to be less promising as evidenced by its poor intracellular uptake, instability in vivo, and nonspecific immune stimulations. Recently, we have demonstrated that single-walled carbon nanotube (SWNT)-mediated siRNA delivery can enhance the efficiency of siRNA-mediated gastrin-releasing peptide receptor (GRP-R) gene silencing by stabilizing siRNA while selectively targeting tumor tissues. Based on our recent findings, we introduce a novel technique to silence specific gene(s) in human neuroblastoma through SWNT-mediated siRNA delivery in vitro and in vivo.
authors.affiliation: Department of Pediatric Surgery, Vanderbilt University Medical center, Nashville, TN, USA.
authors.forename: Jingbo
authors.initials: J
authors.lastname: Qiao
authors.forename: Tu
authors.initials: T
authors.lastname: Hong
authors.forename: Honglian
authors.initials: H
authors.lastname: Guo
authors.forename: Ya-Qiong
authors.initials: YQ
authors.lastname: Xu
authors.forename: Dai H
authors.initials: DH
authors.lastname: Chung
mesh_terms: D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002471:Cell Transformation, Neoplastic; D004337:Drug Carriers / Q000737:chemistry / Q000633:toxicity; D020868:Gene Silencing; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D057054:Molecular Imaging; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000633:toxicity; D009447:Neuroblastoma / Q000473:pathology*; D034741:RNA, Small Interfering / Q000737:chemistry* / Q000235:genetics*; D018004:Receptors, Bombesin / Q000172:deficiency* / Q000235:genetics*; D014162:Transfection
chemical_list: D004337:Drug Carriers; D037742:Nanotubes, Carbon; D034741:RNA, Small Interfering; D018004:Receptors, Bombesin