title: Synthesis and structure of 16,22-diketocholesterol bound to oxysterol-binding protein Osh4.
abstract: We have synthesized 16,22-diketocholesterol, a novel ligand for oxysterol-binding protein Osh4, and determined X-ray structure of the diketocholesterol in complex with Osh4. The X-ray structure shows that α7 helix of Osh4 assumes open conformation while the rest of Osh4, closed conformation, implying this diketocholesterol-bound Osh4 structure may represent a structural intermediate between the two conformations.
authors.affiliation: Division of Medicinal Chemistry, College of Pharmacy, The University of Texas at Austin, Austin, TX 78712, USA.
authors.forename: Myong Chul
authors.initials: MC
authors.lastname: Koag
authors.forename: Young
authors.initials: Y
authors.lastname: Cheun
authors.forename: Yi
authors.initials: Y
authors.lastname: Kou
authors.forename: Hala
authors.initials: H
authors.lastname: Ouzon-Shubeita
authors.forename: Kyungjin
authors.initials: K
authors.lastname: Min
authors.forename: Arthur F
authors.initials: AF
authors.lastname: Monzingo
authors.forename: Seongmin
authors.initials: S
authors.lastname: Lee
mesh_terms: D001665:Binding Sites; D002784:Cholesterol / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D018360:Crystallography, X-Ray; D006860:Hydrogen Bonding; D057927:Hydrophobic and Hydrophilic Interactions; D008565:Membrane Proteins / Q000737:chemistry*; D008958:Models, Molecular; D011485:Protein Binding; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011987:Receptors, Steroid / Q000737:chemistry*; D012441:Saccharomyces cerevisiae; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*; D000098825:Oxysterol Binding Proteins
chemical_list: D002784:Cholesterol; D008565:Membrane Proteins; D011987:Receptors, Steroid; D029701:Saccharomyces cerevisiae Proteins; C586241:16,22-diketocholesterol; C087901:KES1 protein, S cerevisiae; D000098825:Oxysterol Binding Proteins
keywords: Osh4; Oxysterol-binding proteins; Oxystrerols