title: Enhanced attachment of human osteoblasts on NH3-treated poly(L-lactic acid) membranes for guided bone regeneration.
abstract: Barrier membranes for guided bone regeneration (GBR) were prepared by a solvent casting method using solutions of poly(L-lactic acid) (PLLA) and chitosan. PLLA and PLLA/chitosan membranes were treated with ammonia gas plasma. PLLA/chitosan membranes were successfully fabricated, and the surface of the PLLA/chitosan membrane was clearly modified by NH3 plasma treatment according to attenuated total reflectance (ATR-FTIR), X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM) analyses. Additionally, water contact angle testing indicated that the hydrophilicity of these membranes was significantly increased. MG-63 cells were cultured on each type of membrane, and cell viability was examined using an MTT assay. After one week of culturing, MG-63 cells were more abundant on PLLA/chitosan membranes than on PLLA membranes. The cell viability of PLLA/chitosan membranes with plasma treatment was significantly higher than that of PLLA membranes. These results suggest that this plasma-treated membrane is suitable for GBR and is a promising source of bioactive membrane material for bone regeneration.
authors.affiliation: Department of Dental Engineering, Chosun University, Gwangju 501-759, Korea.
authors.forename: Ju-Hee
authors.initials: JH
authors.lastname: Byeon
authors.forename: Su-Gwan
authors.initials: SG
authors.lastname: Kim
authors.forename: Jun-Sik
authors.initials: JS
authors.lastname: Son
authors.forename: Seung-Chan
authors.initials: SC
authors.lastname: Jin
authors.forename: Zheng-Gang
authors.initials: ZG
authors.lastname: Piao
authors.forename: Sook-Young
authors.initials: SY
authors.lastname: Lee
authors.forename: Eun-Sook
authors.initials: ES
authors.lastname: Jang
authors.forename: Jae-Sung
authors.initials: JS
authors.lastname: Kim
authors.forename: Mi-Ae
authors.initials: MA
authors.lastname: Jeong
authors.forename: Hoon
authors.initials: H
authors.lastname: Ahn
authors.forename: Jong-Phil
authors.initials: JP
authors.lastname: Park
mesh_terms: D000641:Ammonia / Q000737:chemistry*; D001861:Bone Regeneration; D002448:Cell Adhesion; D002460:Cell Line; D048091:Guided Tissue Regeneration / Q000295:instrumentation*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000166:cytology*; D056951:Photoelectron Spectroscopy; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D008567:Membranes, Artificial; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D000641:Ammonia