title: Determination and pharmacokinetics of amygdalin in rats by LC-MS-MS.
abstract: A sensitive and specific liquid chromatography-tandem mass spectrometric (LC-MS-MS) method was developed for the determination and pharmacokinetics of amygdalin in rats. Rat plasma pretreated by solid-phase extraction was analyzed by LC-MS-MS with negative electrospray ionization in the multiple reaction monitoring mode. Amygdalin and geniposide [the internal standard (IS)] were separated on a C18 column eluted with a mobile phase of methanol and water (85:15; v/v) at a flow rate of 0.25 mL/min in a run time of 3.0 min. The precursor to product ion transitions were monitored at m/z 457.2 → 279.1 for amygdalin and m/z 387.1 → 224.9 for the IS. The calibration curve of amygdalin showed good linearity over a concentration range of 10-2,000 ng/mL. The limit of quantification was 10 ng/mL. Intra-day and inter-day precisions and accuracy (percent relative standard deviation) were both within 10%. The method was fully validated for its selectivity, sensitivity, matrix effect, recovery and stability. This accurate and specific assay produced a useful LC-MS-MS method, which was successfully applied to pharmacokinetic studies after the oral administration of amygdalin to rats.
authors.affiliation: Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, No.12, Jichang Road, Guangzhou 510405, Guangdong Province, China.
authors.forename: Xiao-bo
authors.initials: XB
authors.lastname: Li
authors.affiliation: Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, No.12, Jichang Road, Guangzhou 510405, Guangdong Province, China.
authors.forename: Chang-hui
authors.initials: CH
authors.lastname: Liu
authors.affiliation: Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, No.12, Jichang Road, Guangzhou 510405, Guangdong Province, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, No.12, Jichang Road, Guangzhou 510405, Guangdong Province, China huangxiaotao@gzucm.edu.cn.
authors.forename: Xiao-tao
authors.initials: XT
authors.lastname: Huang
authors.affiliation: Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, No.12, Jichang Road, Guangzhou 510405, Guangdong Province, China.
authors.forename: Ying-yi
authors.initials: YY
authors.lastname: Li
authors.affiliation: Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, No.12, Jichang Road, Guangzhou 510405, Guangdong Province, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Han
authors.affiliation: Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, No.12, Jichang Road, Guangzhou 510405, Guangdong Province, China.
authors.forename: Mei-li
authors.initials: ML
authors.lastname: Xu
authors.affiliation: Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, No.12, Jichang Road, Guangzhou 510405, Guangdong Province, China.
authors.forename: Sui-qing
authors.initials: SQ
authors.lastname: Mi
authors.affiliation: Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, No.12, Jichang Road, Guangzhou 510405, Guangdong Province, China.
authors.forename: Ning-sheng
authors.initials: NS
authors.lastname: Wang
mesh_terms: D000678:Amygdalin / Q000097:blood* / Q000493:pharmacokinetics*; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000678:Amygdalin