title: Generation of priming mesenchymal stem cells with enhanced potential to differentiate into specific cell lineages using extracellular matrix proteins.
abstract: Poor understanding of the differentiation of mesenchymal stem cells (MSCs) has resulted in a low differentiation yield, and has hindered their application in medicine. As a solution, priming MSCs sensitive to signaling, thus stimulating differentiation into a specific cell lineage, may improve the differentiation yield. To demonstrate this, priming MSCs were produced by using a gelatin matrix for the isolation of primary MSCs from bone-marrow-derived primary cells. Subsequently, cellular characteristics and sensitivity to specific differentiation signals were analyzed at passage five. Compared to non-priming MSCs, priming MSCs showed no significant differences in cellular characteristics, but demonstrated a significant increase in sensitivity to neurogenic differentiation signals. These results demonstrate that generation of priming MSCs by specific extracellular signaling increases the rate of differentiation into a cell-specific lineage.
authors.affiliation: Department of Animal Biotechnology, College of Animal Life and Science, Kangwon National University, Chuncheon 200-701, South Korea.
authors.forename: Na Rae
authors.initials: NR
authors.lastname: Han
authors.forename: Jung Im
authors.initials: JI
authors.lastname: Yun
authors.forename: Young Hyun
authors.initials: YH
authors.lastname: Park
authors.forename: Ji Yeon
authors.initials: JY
authors.lastname: Ahn
authors.forename: Choonghyo
authors.initials: C
authors.lastname: Kim
authors.forename: Jung Hoon
authors.initials: JH
authors.lastname: Choi
authors.forename: Eunsong
authors.initials: E
authors.lastname: Lee
authors.forename: Jeong Mook
authors.initials: JM
authors.lastname: Lim
authors.forename: Seung Tae
authors.initials: ST
authors.lastname: Lee
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001853:Bone Marrow / Q000378:metabolism; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D048430:Cell Shape; D016326:Extracellular Matrix Proteins / Q000378:metabolism*; D005780:Gelatin / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010012:Osteogenesis; D061251:Primary Cell Culture; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction
chemical_list: D015415:Biomarkers; D016326:Extracellular Matrix Proteins; D005780:Gelatin
keywords: Bone-marrow-derived mesenchymal stem cells; Differentiation; Extracellular matrix proteins; Gelatin; Neurogenic-lineage cells