title: New flavonol triglycosides from the leaves of soybean cultivars.
abstract: Soybean (Glycine max) is a major crop in the world. Three new flavonol 3-O-glycosides, kaempferol 3-O-alpha-L-rhamnopyranosyl-(1 --> 4)-[alpha-L-rhamnopyranosyl-(1 --> 6)-beta-D-galactopyranoside] (1), kaempferol 3-O-alpha-L-rhamnopyranosyl-(1 --> 4)-[beta-D-glucopyranosyl-(1 --> 6)-beta-D-galactopyranoside] (4) and quercetin 3-O-beta-D-glucopyranosyl-(1--> 2)-[alpha-L-rhamnopyranosyl-(1 --> 6)-beta-galactopyranoside] (5) were isolated from the leaves of soybean cultivars, together with three known compounds, kaempferol 3-O-beta-D-glucopyranosyl-(1 --> 2)-[alpha-L-rhamnopyranosyl-(1--> 6)-beta-D-galactopyranoside] (2), kaempferol 3-O-beta-D-glucopyranosyl-(1 --> 2)-[alpha-L-rhamnopyranosyl-(1 --> 6)-beta-D-glucopyranoside] (3) and quercetin 3-O-beta-D-glucopyranosyl-(1 --> 2)-[alpha-L-rhamnopyranosyl-(1 --> 6)-beta-D-glucopyranoside] (6), and also common flavonoids. The isolated compounds possess similar structures and high water solubility, and so it was hard to isolate them (in particular 5 and 6) with a normal preparative HPLC system. Their final purification was achieved by a preparative HPLC system equipped with a recycle device.
authors.affiliation: Department of Chemistry, Hiyoshi Campus, Keio University, 4-1-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa 223-8521, Japan. murai@kahaku.go.jp
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Murai
authors.forename: Ryoji
authors.initials: R
authors.lastname: Takahashi
authors.forename: Felipe Rojas
authors.initials: FR
authors.lastname: Rodas
authors.forename: Junichi
authors.initials: J
authors.lastname: Kitajima
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Iwashina
mesh_terms: D002851:Chromatography, High Pressure Liquid; D044948:Flavonols / Q000737:chemistry / Q000302:isolation & purification*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification*; D018515:Plant Leaves / Q000737:chemistry; D013025:Glycine max / Q000737:chemistry*
chemical_list: D044948:Flavonols; D006027:Glycosides