title: [Prevalence of occult hepatitis B virus infection and its phylogenetic features among mother-teenager pairs].
abstract: OBJECTIVE
  Prevalence of occult hepatitis B virus (HBV) infection (OBI) was investigated in a paired mother-teenager population and HBV S gene variation including overt and occult HBV, was determined.
  
  
  METHODS
  A follow-up study based on an initial survey of 135 mother-teenager pairs was carried out through collection of questionnaires and blood samples HBsAg were detected by ELISA method, viral load by PCR amplification and HBV S gene by phylogenetic analysis.
  
  
  RESULTS
  102 pairs of subjects were followed-up. Blood samples from 94 mothers and 101 children were collected. OBI prevalence in mothers was 10.0% (6/60), significantly higher than 2.0% (2/101) in teenagers. Medians of viral load were 399.9 IU/ml and 247.6 IU/ml in overt and occult HBV strains, but without significant difference. 1 occult HBV strain belonged to genotype B with serotype adw while the other 7 were genotype C with serotype adr. 15 of the overt HBV strains belonged to genotype B with serotype adw and the other 8 were genotype C with serotype adr. Proportions of genotype-C strains were significantly higher in occult HBV strains than in overt HBV strains.
  
  
  CONCLUSIONS
  OBI was seen in teenage-mother population.
authors.affiliation: Deqing County Center for Disease Control and Prevention, Huzhou 313200, China.
authors.forename: Xiao-lian
authors.initials: XL
authors.lastname: Dong
authors.forename: Qing-qing
authors.initials: QQ
authors.lastname: Yao
authors.forename: Xue-cai
authors.initials: XC
authors.lastname: Wang
authors.forename: Hai-tao
authors.initials: HT
authors.lastname: Xu
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Wang
authors.forename: Sheng-yu
authors.initials: SY
authors.lastname: Chen
authors.forename: Zhi-feng
authors.initials: ZF
authors.lastname: Tang
authors.forename: Ying-Jie
authors.initials: YJ
authors.lastname: Zheng
mesh_terms: D000293:Adolescent; D000328:Adult; D004279:DNA, Viral / Q000097:blood*; D005260:Female; D005838:Genotype; D006509:Hepatitis B / Q000097:blood / Q000453:epidemiology* / Q000821:virology*; D006514:Hepatitis B Surface Antigens / Q000097:blood; D006515:Hepatitis B virus / Q000145:classification / Q000235:genetics*; D006801:Humans; D008297:Male; D009035:Mothers; D010802:Phylogeny; D019562:Viral Load
chemical_list: D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens