title: [Expression of P75NTR in the testis of nestin-GFP transgenic mice].
abstract: OBJECTIVE
  To explore the P75NTR expression in the mouse testis and its relationship with nestin.
  
  
  METHODS
  We observed the location of the expressions of P75NTR and nestin in the testis of the nestin-GFP transgenic mouse on postnatal day (PND) 5, 14 and 30 using immunofluorescence, and detected the expression levels of P75NTR in the testicular tissue of mice in different age groups by real-time quantitative PCR (RTqPCR) and flow cytometry. Then we cultured the P75NTR positive cells in neural stem cell culture medium and observed their neuronal differentiation capacity by orientation differentiation.
  
  
  RESULTS
  Immunofluorescence showed the expressions of P75NTR and nestin in the Leydig cells of the mouse testis. RTqPCR and flow cytometry exhibited the peak of the P75NTR expression on PND 14. The positive rates of P75NTR were (2.88 +/- 0.52), (9.54 +/- 1.81) and (2.63 +/- 0.43)% on PND 5, 14 and 30, respectively. The P75NTR positive cells obtained also expressed nestin and P75NTR and had the capacity of neuronal differentiation.
  
  
  CONCLUSIONS
  P75NTR and nestin are co-expressed in the Leydig cells of the mouse testis, and the P75NTR positive cells have the ability of neural differentiation, which is presumably attributed to neural crest cells.
authors.affiliation: Center for Stem Cell Biology and Tissue Engineering, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510080, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Tuo
authors.forename: Mei-Hua
authors.initials: MH
authors.lastname: Jiang
authors.forename: Bing
authors.initials: B
authors.lastname: Cai
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Deng
authors.forename: Peng
authors.initials: P
authors.lastname: Xiang
mesh_terms: D000818:Animals; D007381:Intermediate Filament Proteins / Q000235:genetics* / Q000378:metabolism; D007985:Leydig Cells / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D064231:Nestin; D020800:Receptor, Nerve Growth Factor / Q000235:genetics* / Q000378:metabolism; D013737:Testis / Q000166:cytology / Q000378:metabolism
chemical_list: D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; D020800:Receptor, Nerve Growth Factor