title: [Prevalence of Anaplasma spp. infection in a desert landscape region of Heshuo, Xinjiang].
abstract: OBJECTIVE
  To study the prevalence of Anaplasmosis among human, domestic sheep and tick population in Heshuo area, southern Xinjiang and to investigate the diversity of Anaplasma species.
  
  
  METHODS
  Ticks were captured from wild field and blood samples were collected from healthy residents and their domestic sheep. Indirect fluorescent assay was carried out to determine the presence of Anaplasma specific IgG antibodies in blood sample of human and goats, respectively. Nested PCR and sequence alignment of Anaplasma partial 16S rDNA were used to investigate the diversity of Anaplasma species.
  
  
  RESULTS
  43.31% (55/127) of human beings and 27.50% (55/200) of the goats were found positive for Anaplasma specific IgG. In total, 367 ticks were captured, including 3 genus and 4 species, which mainly consisting of dominate Hyalomma (H.) asiaticum (47.41%) and Rhipicephalus (R.) pumilio (37.60%). 5.00% (18/360) of the questing ticks and 4.49% (7/156) of the goat blood samples were found to have had 16S rDNA, representing Anaplasma sp. by nested PCR, but none was found from human beings. Results from sequential alignment revealed that the positive amplicons were identified to be Anaplasma phagocytophilum (99.2% GU046565, 99.5% GU064897 and 99.5% AB196721) and Anaplasma central (99.2% GU064903).
  
  
  CONCLUSIONS
  Human and zoonotic anaplasmosis, which were probably transmitted by H. asiaticum and R. pumilio, were co-circulating in the desert landscape of Heshuo area, Xinjiang.
authors.affiliation: Centers for Disease Control and Prevention of Xinjiang Military Command Area, Urumqi, China. xjglzhang@126.com
authors.forename: Gui-lin
authors.initials: GL
authors.lastname: Zhang
authors.forename: Xiang
authors.initials: X
authors.lastname: Sun
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Xiao-min
authors.initials: XM
authors.lastname: Liu
authors.forename: Zhong
authors.initials: Z
authors.lastname: Zheng
authors.forename: Yi
authors.initials: Y
authors.lastname: Sun
authors.forename: Ran
authors.initials: R
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D000709:Anaplasma; D000711:Anaplasmataceae Infections / Q000453:epidemiology / Q000662:veterinary*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D002681:China / Q000453:epidemiology; D003889:Desert Climate; D005260:Female; D005843:Geography; D015511:Goat Diseases / Q000453:epidemiology / Q000382:microbiology; D006041:Goats / Q000382:microbiology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D008875:Middle Aged; D008913:Minority Groups; D015995:Prevalence; D012756:Sheep / Q000382:microbiology*; D012757:Sheep Diseases / Q000453:epidemiology / Q000382:microbiology; D017282:Tick-Borne Diseases / Q000453:epidemiology* / Q000382:microbiology; D013987:Ticks / Q000382:microbiology; D055815:Young Adult
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G