title: Associations between insulin-like growth factor I, vascular endothelial growth factor and its soluble receptor 1 in umbilical serum and endothelial cells obtained from normotensive and preeclamptic pregnancies.
abstract: The aim of this study was to investigate the associations between insulin-like growth factor I (IGF-I) with vascular endothelial growth factor (VEGF) and its soluble receptor 1 (sFlt-1) in umbilical serum and to study the effects of IGF-I upon sFlt-1 synthesis in human umbilical vein endothelial cells (HUVEC) in normotensive (NT) and preeclamptic (PE) pregnancies. As compared with the NT group, umbilical serum IGF-I and VEGF levels were lower in the PE group, while sFlt-1 concentrations were higher. Levels of sFlt-1 correlated with IGF-I in the NT group and with VEGF in the PE group. Basal concentration of sFlt-1 in HUVEC culture media was higher in the PE group. IGF-I stimulated sFlt-1 synthesis only in the NT group. In summary, umbilical serum sFlt-1 is associated with IGF-I in normotensive pregnancy and with VEGF in preeclampsia. IGF-I stimulates sFlt-1 synthesis in endothelial cells in normotensive but not in PE pregnancies.
authors.affiliation: Department of Reproductive Biology Carlos Gual Castro, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Tlalpan, DF México.
authors.forename: Andrea
authors.initials: A
authors.lastname: Olmos
authors.forename: Lorenza
authors.initials: L
authors.lastname: Díaz
authors.forename: Euclides
authors.initials: E
authors.lastname: Avila
authors.forename: David
authors.initials: D
authors.lastname: Barrera
authors.forename: Rebeca
authors.initials: R
authors.lastname: López-Marure
authors.forename: Benjamín
authors.initials: B
authors.lastname: Biruete
authors.forename: Fernando
authors.initials: F
authors.lastname: Larrea
authors.forename: Ali
authors.initials: A
authors.lastname: Halhali
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005260:Female; D005312:Fetal Blood / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism; D006801:Humans; D007231:Infant, Newborn; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000097:blood / Q000378:metabolism*; D011247:Pregnancy; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*; D040281:Vascular Endothelial Growth Factor Receptor-1 / Q000378:metabolism*
chemical_list: C583557:IGF1 protein, human; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D007334:Insulin-Like Growth Factor I; C501162:FLT1 protein, human; D040281:Vascular Endothelial Growth Factor Receptor-1