title: [Changes of extracellular matrix of nasal mucosa in guinea pig model of prolonged allergic-induced rhinitis].
abstract: OBJECTIVE
  The aim of this study was to explore the changes of the extracellular matrix in nasal mucosa by a guinea pig model of prolonged allergic-induced rhinitis.
  
  
  METHODS
  Thirty-two male Hartley guinea pigs were randomly divided into four groups: allergen challenged groups (Group 2 w, Group 6 w and Group 12 w) and a control group. Ovalbumin-sensitized guinea pigs were repeatedly challenged with allergen twice a week from 2 weeks to 12 weeks. Matched control groups were challenged with physiological saline. Nasal mucosa were obtained from the animals killed. Hematoxylin-Eosin, Masson's trichrome, and immunohistochemical staining against transforming growth factor-beta1 (TGF-beta1), Collagen III and Collagen I were performed to nasal mucosa.
  
  
  RESULTS
  (1) Pathological examination showed obvious infiltration of eosinophils and the enlarged thickness of epithelial layer of nasal mucosa in the experiment groups. (2) The area ratios of blue stained in the extracellular matrix of nasal mucosa were increased. The area ratios of blue stained were statistically different in Group 6 w and Group 12 w compared with the control group. (3) The increasing absorbance of TGF-beta1 were statistically different in the experiment groups with the control group. The absorbance of Collagen III and Collagen I showed a rising trend along prolonged allergen challenged in the experiment groups.
  
  
  CONCLUSIONS
  Prolonged allergen challenge and the inflammation of nasal mucosa, can lead to the increasing of the inflammation relevant factors and the deposit of collagen in the extracellular matrix of nasal mucosa.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Nanjing General Hospital of Nanjing Military Command, Nanjing, 210002, China. jiangmjdoc@163.com
authors.forename: Manjie
authors.initials: M
authors.lastname: Jiang
authors.forename: Zhiyi
authors.initials: Z
authors.lastname: Wang
authors.forename: Jinrong
authors.initials: J
authors.lastname: Wu
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Mei
authors.initials: M
authors.lastname: Zhou
authors.forename: Tianyou
authors.initials: T
authors.lastname: Wang
authors.forename: Zeqing
authors.initials: Z
authors.lastname: Li
authors.forename: Qiuping
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000485:Allergens / Q000276:immunology; D000818:Animals; D024042:Collagen Type I / Q000378:metabolism; D024061:Collagen Type III / Q000378:metabolism; D004804:Eosinophils / Q000276:immunology; D005109:Extracellular Matrix / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D006168:Guinea Pigs; D007249:Inflammation; D008297:Male; D009297:Nasal Mucosa / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D012221:Rhinitis, Allergic, Perennial / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
chemical_list: D000485:Allergens; D024042:Collagen Type I; D024061:Collagen Type III; D053773:Transforming Growth Factor beta1