title: Evaluation of oxidative stress and DNA damage in traffic policemen exposed to vehicle exhaust.
abstract: OBJECTIVE
  We aimed to study the genotoxic effects in traffic police who are occupationally exposed due to higher free radical generation.
  
  
  METHODS
  Ambient and breathing zone air samples were analyzed blood samples were collected for analysis of antioxidant enzymes Superoxide Dismutase (SOD), Glutathione Peroxidase (GPx) and free radicals - nitric oxide (NO) and malondialdehyde (MDA) levels using a spectrophotometer. DNA damage was measured with the comet assay.
  
  
  RESULTS
  Higher levels of benzene (BZ), toluene (TOL), carbon monoxide (CO), benzo([a])pyrene (BaP) and sulfur dioxide (SO2) was observed in traffic police. Elevated levels of NO, MDA and comet tail length and lower SOD and GPx levels observed in traffic police.
  
  
  CONCLUSIONS
  The studied biomarkers, related to oxidative stress and DNA damage positively correlated in traffic police exposed to environmental air pollutants.
authors.affiliation: Department of Environmental Toxicology, Institute of Genetics and Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad, Andhra Pradesh, India.
authors.forename: Badabagni Siva
authors.initials: BS
authors.lastname: Prasad
authors.forename: Peddireddy
authors.initials: P
authors.lastname: Vidyullatha
authors.forename: Rekhadevi Perumalla
authors.initials: RP
authors.lastname: Venkata
authors.forename: Vani Gudimella
authors.initials: VG
authors.lastname: Tirumala
authors.forename: Sreedevi
authors.initials: S
authors.lastname: Varre
authors.forename: Usha Rani
authors.initials: UR
authors.lastname: Penagaluru
authors.forename: Paramjit
authors.initials: P
authors.lastname: Grover
authors.forename: Hema Prasad
authors.initials: HP
authors.lastname: Mundluru
authors.forename: Pardhanandana Reddy
authors.initials: PR
authors.lastname: Penagaluru
mesh_terms: D000328:Adult; D000393:Air Pollutants / Q000633:toxicity*; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D004249:DNA Damage; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D009569:Nitric Oxide / Q000097:blood; D016273:Occupational Exposure; D018384:Oxidative Stress; D016495:Police; D013482:Superoxide Dismutase / Q000097:blood; D001335:Vehicle Emissions / Q000633:toxicity*; D055815:Young Adult
chemical_list: D000393:Air Pollutants; D015415:Biomarkers; D001335:Vehicle Emissions; D009569:Nitric Oxide; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase