title: Structural and physicochemical property relationships of cruciferin homohexamers.
abstract: Heteromeric cruciferin from wild type (WT) Arabidopsis thaliana and homomeric cruciferin CRUA, CRUB, and CRUC composed of identical subunits obtained from double-knockout mutant lines were investigated for their structural and physicochemical properties. A three-step chromatographic procedure allowed isolation of intact cruciferin hexamers with high purity (>95%). FT-IR and CD analysis of protein secondary structure composition revealed that all cruciferins were folded into higher order structures consisting of 44-50% β-sheets and 7-9% α-helices. The structural and physicochemical properties of homohexameric CRUC deviated from that of CRUA and CRUB and exhibited a compact, thermostable, and less hydrophobic structure, confirming the predictions made using 3D homology structure models.
authors.affiliation: Agriculture and Agri-Food Canada , Saskatoon, Saskatchewan, S7N 0X2 Canada.
authors.forename: Thushan S
authors.initials: TS
authors.lastname: Withana-Gamage
authors.forename: Dwayne D
authors.initials: DD
authors.lastname: Hegedus
authors.forename: Xiao
authors.initials: X
authors.lastname: Qiu
authors.forename: Tara
authors.initials: T
authors.lastname: McIntosh
authors.forename: Janitha P D
authors.initials: JP
authors.lastname: Wanasundara
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D055598:Chemical Phenomena; D055786:Gene Knockout Techniques; D005916:Globulins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D050505:Mutant Proteins / Q000737:chemistry / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism*; D011487:Protein Conformation; D055550:Protein Stability; D021122:Protein Subunits / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D055314:Seed Storage Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D012639:Seeds / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D005916:Globulins; D050505:Mutant Proteins; D021122:Protein Subunits; D055314:Seed Storage Proteins; C571789:cruciferin A, Arabidopsis; C506890:cruciferin B, Arabidopsis; C506891:cruciferin C, Arabidopsis