title: Antitumor activity of polysaccharides from Lepista sordida against laryngocarcinoma in vitro and in vivo.
abstract: Four water-soluble polysaccharides (LSPa1, LSPb1, LSPb2 and LSPc1) extracted from the fruiting bodies of Lepista sordida were prepared by DEAE cellulose-52 anion-exchange and Sepharose 6 Fast Flow gel-permeation chromatography. LSPa1 was composed of Glc, Man and Gal with a molar ratio of 3.2: 1.3: 0.6. LSPb1, LSPb2 and LSPc1 were composed of Glc, Man, Gal, Rha and Ara in the proportions of 2.6: 2.1: 0.9: 0.6: 0.3, 1.2: 1.0: 2.3: 0.6: 0.5, 0.2: 0.3: 2.3: 0.3: 2.0, respectively. The high-performance gel-permeation chromatography (HPGPC) analysis showed that the average molecular weight (Mw) of four polysaccharides were approximately 156, 134, 96 and 57 kDa, respectively. MTT and lactate dehydrogenase (LDH) assays indicated that three acidic polysaccharides, namely LSPb1, LSPb2 and LSPc1, possessed more potent antiproliferative effect on human laryngocarcinoma Hep-2 cells than neutral polysaccharide LSPa1 in a dose- and time-dependent manner and the effect increased in the order of LSPa1<LSPb2<LSPb1<LSPc1, which is the same as their uronic acid content. Furthermore the tumor volume and tumor weight were also obviously suppressed after two weeks' treatment of LSPc1 to mice engrafted with Hep-2 cancer cells. Results of these studies demonstrated that the polysaccharide LSPc1 had a potential application as natural antitumor drugs.
authors.affiliation: Department of Head and Neck Surgery, the Third Affiliated Hospital of Harbin Medical University, Harbin 150081, China.
authors.forename: Susheng
authors.initials: S
authors.lastname: Miao
authors.forename: Xionghui
authors.initials: X
authors.lastname: Mao
authors.forename: Rong
authors.initials: R
authors.lastname: Pei
authors.forename: Suping
authors.initials: S
authors.lastname: Miao
authors.forename: Cheng
authors.initials: C
authors.lastname: Xiang
authors.forename: Yuanjing
authors.initials: Y
authors.lastname: Lv
authors.forename: Xianguang
authors.initials: X
authors.lastname: Yang
authors.forename: Ji
authors.initials: J
authors.lastname: Sun
authors.forename: Shenshan
authors.initials: S
authors.lastname: Jia
authors.forename: Yvpeng
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D002277:Carcinoma / Q000188:drug therapy / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D005260:Female; D062610:Fungal Polysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000188:drug therapy / Q000473:pathology*; D051379:Mice; D047368:Tumor Burden / Q000187:drug effects; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D062610:Fungal Polysaccharides
keywords: Antitumor; Bioassay-guided isolation; Laryngocarcinoma; Lepista sordida; Polysaccharide