title: Adult bone marrow neural crest stem cells and mesenchymal stem cells are not able to replace lost neurons in acute MPTP-lesioned mice.
abstract: Adult bone marrow stroma contains multipotent stem cells (BMSC) that are a mixed population of mesenchymal and neural-crest derived stem cells. Both cells are endowed with in vitro multi-lineage differentiation abilities, then constituting an attractive and easy-available source of material for cell therapy in neurological disorders. Whereas the in vivo integration and differentiation of BMSC in neurons into the central nervous system is currently matter of debate, we report here that once injected into the striatum of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated mice, pure populations of either bone marrow neural crest stem cells (NCSC) or mesenchymal stem cells (MSC) survived only transiently into the lesioned brain. Moreover, they do not migrate through the brain tissue, neither modify their initial phenotype, while no recovery of the dopaminergic system integrity was observed. Consequently, we tend to conclude that MSC/NCSC are not able to replace lost neurons in acute MPTP-lesioned dopaminergic system through a suitable integration and/or differentiation process. Altogether with recent data, it appears that neuroprotective, neurotrophic and anti-inflammatory features characterizing BMSC are of greater interest as regards CNS lesions management.
authors.affiliation: Groupe Interdisciplinaire de Génoprotéomique Appliquée (GIGA), Unit of Neurosciences, University of Liege, Liège, Belgium.
authors.forename: Virginie
authors.initials: V
authors.lastname: Neirinckx
authors.forename: Alice
authors.initials: A
authors.lastname: Marquet
authors.forename: Cécile
authors.initials: C
authors.lastname: Coste
authors.forename: Bernard
authors.initials: B
authors.lastname: Rogister
authors.forename: Sabine
authors.initials: S
authors.lastname: Wislet-Gendebien
mesh_terms: D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000502:physiology; D001930:Brain Injuries / Q000139:chemically induced / Q000473:pathology / Q000628:therapy*; D016923:Cell Death; D059290:Dopaminergic Neurons / Q000473:pathology; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D058953:Neural Stem Cells / Q000166:cytology* / Q000502:physiology; D033581:Stem Cell Transplantation; D017211:Treatment Failure
chemical_list: D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine