title: Low-Level Laser-Accelerated Peripheral Nerve Regeneration within a Reinforced Nerve Conduit across a Large Gap of the Transected Sciatic Nerve in Rats.
abstract: This study proposed a novel combination of neural regeneration techniques for the repair of damaged peripheral nerves. A biodegradable nerve conduit containing genipin-cross-linked gelatin was annexed using beta-tricalcium phosphate (TCP) ceramic particles (genipin-gelatin-TCP, GGT) to bridge the transection of a 15 mm sciatic nerve in rats. Two trigger points were irradiated transcutaneously using 660 nm of gallium-aluminum arsenide phosphide (GaAlAsP) via laser diodes for 2 min daily over 10 consecutive days. Walking track analysis showed a significant improvement in sciatic functional index (SFI) (P < 0.01) and pronounced improvement in the toe spreading ability of rats undergoing laser stimulation. Electrophysiological measurements (peak amplitude and area) illustrated by compound muscle action potential (CMAP) curves demonstrated that laser stimulation significantly improved nerve function and reduced muscular atrophy. Histomorphometric assessments revealed that laser stimulation accelerated nerve regeneration over a larger area of neural tissue, resulting in axons of greater diameter and myelin sheaths of greater thickness than that observed in rats treated with nerve conduits alone. Motor function, electrophysiological reactions, muscular reinnervation, and histomorphometric assessments all demonstrate that the proposed therapy accelerated the repair of transected peripheral nerves bridged using a GGT nerve conduit.
authors.affiliation: Department of Neurosurgery, Taichung Veterans General Hospital, Taichung 40705, Taiwan ; Department of Medicine, National Defense Medical Center, Taipei 114, Taiwan ; Tri-Service General Hospital, National Defense Medical Center, Taipei 114, Taiwan ; Department of Physical Therapy, Hungkuang University, Taichung 43302, Taiwan.
authors.forename: Chiung-Chyi
authors.initials: CC
authors.lastname: Shen
authors.forename: Yi-Chin
authors.initials: YC
authors.lastname: Yang
authors.forename: Tsung-Bin
authors.initials: TB
authors.lastname: Huang
authors.forename: Shiuh-Chuan
authors.initials: SC
authors.lastname: Chan
authors.forename: Bai-Shuan
authors.initials: BS
authors.lastname: Liu