title: Preparation, characterization and antimicrobial efficiency of Ag/PDDA-diatomite nanocomposite.
abstract: Nanocomposites consisting of diatomaceous earth particles and silver nanoparticles (silver NPs) with high antimicrobial activity were prepared and characterized. For the purpose of nanocomposite preparation, silver NPs with an average size of 28nm prepared by modified Tollens process were used. Nanocomposites were prepared using poly(diallyldimethylammonium) chloride (PDDA) as an interlayer substance between diatomite and silver NPs which enables to change diatomite original negative surface charge to positive one. Due to strong electrostatic interactions between negatively charged silver NPs and positively charged PDDA-modified diatomite, Ag/PDDA-diatomite nanocomposites with a high content of silver (as high as 46.6mgAg/1g of diatomite) were prepared. Because of minimal release of silver NPs from prepared nanocomposites to aqueous media (<0.3mg Ag/1g of nanocomposite), the developed nanocomposites are regarded as a potential useful antimicrobial material with a long-term efficiency showing no risk to human health or environment. All the prepared nanocomposites exhibit a high bactericidal activity against Gram-negative and Gram-positive bacteria and fungicidal activity against yeasts at very low concentrations as low as 0.11g/L, corresponding to silver concentration of 5mg/L. Hence, the prepared nanocomposites constitute a promising candidate suitable for the microbial water treatment in environmental applications.
authors.affiliation: Regional Centre of Advanced Technologies and Materials, Department of Physical Chemistry, Faculty of Science, Palacký University in Olomouc, 17. listopadu 12, 771 46 Olomouc, Czech Republic. ales.panacek@upol.cz
authors.forename: Aleš
authors.initials: A
authors.lastname: Panáček
authors.forename: Anna
authors.initials: A
authors.lastname: Balzerová
authors.forename: Robert
authors.initials: R
authors.lastname: Prucek
authors.forename: Václav
authors.initials: V
authors.lastname: Ranc
authors.forename: Renata
authors.initials: R
authors.lastname: Večeřová
authors.forename: Vendula
authors.initials: V
authors.lastname: Husičková
authors.forename: Jiří
authors.initials: J
authors.lastname: Pechoušek
authors.forename: Jan
authors.initials: J
authors.lastname: Filip
authors.forename: Radek
authors.initials: R
authors.lastname: Zbořil
authors.forename: Libor
authors.initials: L
authors.lastname: Kvítek
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D000935:Antifungal Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D007692:Diatomaceous Earth / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D008826:Microbial Sensitivity Tests; D053761:Nanocomposites / Q000737:chemistry*; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D010316:Particle Size; D011095:Polyethylenes / Q000737:chemistry / Q000494:pharmacology*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects*; D012834:Silver / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013499:Surface Properties
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D009942:Organometallic Compounds; D011095:Polyethylenes; D000644:Quaternary Ammonium Compounds; C041004:poly-N,N-dimethyl-N,N-diallylammonium chloride; D012834:Silver; D007692:Diatomaceous Earth; C033787:diatomite