title: [Expression and clinical significance of soluble B7-H3 in sera of patients with primary hepatocellular carcinoma].
abstract: OBJECTIVE
  To detect the expression and clinical significance of co-stimulatory molecule B7-H3 in sera and tissues of patients with hepatocellular carcinoma (HCC).
  
  
  METHODS
  We collected 63 samples of sera and liver tissue from HCC patients, 5 adjacent normal tissues of hepatic hemangioma as controls, and the other 50 sera samples of healthy people in the same time. The expression of soluble B7-H3 (sB7-H3) in sera from HCC patients and healthy people was detected by ELISA. Immunohistochemistry was performed to analyze the expression of B7-H3 on normal liver and HCC tissues.
  
  
  RESULTS
  The level of sB7-H3 from HCC patients was significantly higher than that from healthy people [(4143.47±976.27) pg/mL vs (2076.18±605.42) pg/mL, P<0.05]; and sB7-H3 level was related to clinical stage, distant metastasis and the positive expression of B7-H3 in HCC tissues (P<0.05), while there was no significant difference between its expression and the other clinical pathological parameters, such as the patients' age, gender, histological type, lymphatic metastasis and size of tumor. Furthermore, a positive correlation was found between the level of sB7-H3 and CA19-9 in HCC patients (P<0.05), but it was not associated with AFP and CEA.
  
  
  CONCLUSIONS
  The level of sB7-H3 in HCC patients was significantly higher than that in healthy people, and B7-H3 expression was correlated with clinical pathological indexes, which implicated that detecting the expression of sB7- H3 might be helpful for the diagnosis of primary HCC.
authors.affiliation: Department of Immunology, Binzhou Medical College, Yantai 264003, China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Wang
authors.forename: Guohua
authors.initials: G
authors.lastname: Yu
authors.forename: Tongshen
authors.initials: T
authors.lastname: Liu
authors.forename: Guoyan
authors.initials: G
authors.lastname: Wang
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Guangbo
authors.initials: G
authors.lastname: Zhang
authors.forename: Xiying
authors.initials: X
authors.lastname: Luan
mesh_terms: D000328:Adult; D000368:Aged; D015295:Antigens, Tumor-Associated, Carbohydrate / Q000097:blood; D060887:B7 Antigens / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D002272:Carcinoembryonic Antigen / Q000097:blood; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D005260:Female; D015870:Gene Expression; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D000509:alpha-Fetoproteins / Q000378:metabolism
chemical_list: D015295:Antigens, Tumor-Associated, Carbohydrate; D060887:B7 Antigens; C423237:CD276 protein, human; D002272:Carcinoembryonic Antigen; D000509:alpha-Fetoproteins; C578959:carbohydrate antigen 199, human