title: Geodermatophilus taihuensis sp. nov., isolated from the interfacial sediment of a eutrophic lake.
abstract: A novel actinobacterial strain, 3-wff-81(T), was isolated from interfacial sediment of the eutrophic Taihu Lake in Jiangsu Province (China) and subjected to polyphasic taxonomic characterization. The strain formed pale orange-pigmented colonies comprising rod-shaped cells on R2A agar. Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain 3-wff-81(T) belonged to the genus Geodermatophilus, with Geodermatophilus soli PB34(T) (99.1 % similarity) and Geodermatophilus terrae PB261(T) (98.3 % similarity) as closest relatives. The major fatty acids were 16 : 0 iso, 15 : 0 iso, 17 : 1ω8c and 14 : 0 iso. The predominant menaquinones were MK-9(H4) and MK-9. Polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannosides. The genomic DNA G+C content was 73.2 mol%. DNA-DNA relatedness values with G. soli PB34(T) and G. terrae PB261(T) were 42.8 % and 39.6 %, respectively. Based on the physiological, biochemical and chemotaxonomic data, it is proposed that strain 3-wff-81(T) represents a novel species named Geodermatophilus taihuensis sp. nov. with 3-wff-81(T) ( = CGMCC 1.12303(T) = NBRC 109416(T)) as the type strain.
authors.affiliation: College of Bioengineering, Henan University of Technology, Zhengzhou 450001, Henan Province, China.
authors.forename: Jian-Hang
authors.initials: JH
authors.lastname: Qu
authors.affiliation: College of Bioengineering, Henan University of Technology, Zhengzhou 450001, Henan Province, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Hui
authors.affiliation: Institute of Environmental and Analytical Sciences, College of Chemistry and Chemical Engineering, Henan University, Kaifeng 475004, Henan Province, China.
authors.forename: Jian-Ying
authors.initials: JY
authors.lastname: Qu
authors.affiliation: College of Bioengineering, Henan University of Technology, Zhengzhou 450001, Henan Province, China.
authors.forename: Fu-Fang
authors.initials: FF
authors.lastname: Wang
authors.affiliation: College of Bioengineering, Henan University of Technology, Zhengzhou 450001, Henan Province, China.
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Li
authors.affiliation: College of Bioengineering, Henan University of Technology, Zhengzhou 450001, Henan Province, China.
authors.forename: Yuan-Sen
authors.initials: YS
authors.lastname: Hu
authors.affiliation: College of Bioengineering, Henan University of Technology, Zhengzhou 450001, Henan Province, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Luo
authors.affiliation: College of Bioengineering, Henan University of Technology, Zhengzhou 450001, Henan Province, China.
authors.forename: Jing-Ping
authors.initials: JP
authors.lastname: Cai
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D060106:Lakes / Q000382:microbiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry; D014871:Water Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9