title: Effect of induced mild hypothermia on two pro-inflammatory cytokines and oxidative parameters during experimental acute sepsis.
abstract: This study aimed to determine the effect of induced mild hypothermia (34°C) on the production of two cytokines (interleukin (IL-6) and tumor necrosis factor (TNF)alpha) and reactive nitrogen and oxygen species in plasma and the heart of acutely septic rats. After anesthesia and in conditions of normothermia (38°C) or mild hypothermia (34°C), acute sepsis was induced by cecal ligation and perforation. For each temperature three groups were formed: (1) baseline (blood sample collected at T0 hour), (2) sham (blood sample at T4 hours) and (3) septic (blood sample at T4 hours). At either temperature sepsis induced a significant increase in plasma IL-6, TNF-alpha and HO• concentration, compared with the sham groups (P≤0.016). Compared with the normothermic septic group, septic rats exposed to mild hypothermia showed a mild decrease in TNF-alpha concentration (104±50 pg/ml vs. 215±114 pg/ml; P>0.05) and a significant decrease in IL-6 (1131±402 pg/ml vs. 2494±691 pg/ml, P=0.038). At either temperature sepsis induced no enhancement within the heart of lipoperoxidation (malondialdehyde content) or antioxidant activities (superoxide dismutase and catalase). In conclusion, during acute sepsis, induced mild hypothermia appears to reduce some pro-inflammatory and oxidative responses. This may, in part, explain the beneficial effect of hypothermia on survival duration of septic rats.
authors.affiliation: Laboratoire ORPHY EA 4324, Université Européenne de Bretagne, Brest, France. Karelle.Leon@univ-brest.fr
authors.forename: Karelle
authors.initials: K
authors.lastname: Léon
authors.forename: Christine
authors.initials: C
authors.lastname: Moisan
authors.forename: Aline
authors.initials: A
authors.lastname: Amérand
authors.forename: Gwladys
authors.initials: G
authors.lastname: Poupon
authors.forename: Erwan
authors.initials: E
authors.lastname: L'Her
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D005609:Free Radicals / Q000378:metabolism; D007036:Hypothermia, Induced; D007249:Inflammation / Q000378:metabolism* / Q000628:therapy; D015850:Interleukin-6 / Q000097:blood*; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde; D009206:Myocardium / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D018805:Sepsis / Q000097:blood* / Q000628:therapy*; D013482:Superoxide Dismutase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D015850:Interleukin-6; D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D002374:Catalase; D013482:Superoxide Dismutase