title: Complete genome sequence of Escherichia coli O157:H7 lytic phage JL1.
abstract: A novel virulent phage named JL1 against Escherichia coli O157:H7 was isolated from raw sewage. It was found that JL1 has an icosahedral head and a long flexible non-contractile tail. The complete genome of JL1 is composed of a linear double-stranded DNA of 43,457 base pairs in length, with 54.77 % G+C content and 60 putative open reading frames. Morphology and bioinformatics analysis revealed that phage JL1 is a member of the family Siphoviridae of the order Caudovirales. It is different from previously reported phages of E. coli O157:H7 but is homologous to Sodalis phage SO-1, Shigella phage EP23, Escherichia phage HK578 and Escherichia phage SSL-2009a.
authors.affiliation: Laboratory of Nutrition and Functional Food, Jilin University, 5333 Xi'An Road, Changchun, 130062, People's Republic of China.
authors.forename: Fengguang
authors.initials: F
authors.lastname: Pan
authors.forename: Han
authors.initials: H
authors.lastname: Wu
authors.forename: Jinhua
authors.initials: J
authors.lastname: Liu
authors.forename: Yongxing
authors.initials: Y
authors.lastname: Ai
authors.forename: Xianmei
authors.initials: X
authors.lastname: Meng
authors.forename: Rizeng
authors.initials: R
authors.lastname: Meng
authors.forename: Qingfeng
authors.initials: Q
authors.lastname: Meng
mesh_terms: D003090:Coliphages / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D019295:Computational Biology / Q000379:methods; D004247:DNA / Q000032:analysis / Q000235:genetics; D019453:Escherichia coli O157 / Q000821:virology*; D016679:Genome, Viral; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000821:virology; D017906:Siphoviridae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012722:Sewage; D014764:Viral Proteins; D004247:DNA