title: Agreement between the cell culture titrations of canine minute virus determined by two susceptibility-testing methods.
abstract: The correct diagnosis of canine minute virus is critical in dog breeding. In this study, the Bland Altman test was used to compare the performance of two susceptibility-testing methods, namely polymerase chain reaction (PCR) and indirect immunofluorescence assay (IFA). The agreement between IFA and PCR in monocytes revealed a mean difference of -1752.16 with 95% confidence and an interval ranging from -3229.80 to -274.53 (SD=2325.62). The agreement between IFA and PCR in Walter Reed canine cells (WRCC) revealed a mean difference of -2396.55 with 95% confidence and an interval ranging from -3774.63 to -1018.48 (SD=2168.93). The Bland Altman test confirmed the overall accuracy of PCR vs IFA and the plot showed that all points were not randomly arranged in the range of average ± 1.96 × SD of the differences.
authors.affiliation: Department of Veterinary Medicine, University of Bari, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy. Electronic address: annamaria.pratelli@uniba.it.
authors.forename: A
authors.initials: A
authors.lastname: Pratelli
authors.forename: V
authors.initials: V
authors.lastname: Colao
mesh_terms: D000818:Animals; D052660:Bocavirus; D018929:Cell Culture Techniques; D004285:Dogs; D019084:Fluorescent Antibody Technique, Indirect; D007963:Leukocytes, Mononuclear / Q000821:virology; D010322:Parvoviridae Infections / Q000175:diagnosis / Q000662:veterinary* / Q000821:virology; D016133:Polymerase Chain Reaction; D014776:Virus Cultivation
keywords: Bland Altman test; Bocavirus; Dog; Immunofluorescence; PCR