title: Molecular layer heterotopia of the cerebellar vermis in mutant and transgenic mouse models on a C57BL/6 background.
abstract: C57BL/6 mice exhibit spontaneous cerebellar malformations consisting of heterotopic neurons and glia in the molecular layer of the vermis (Tanaka and Marunouchi, 2005; Mangaru et al., 2013). Malformations are only found between folia VIII and IX and are indicative of deficits of neuronal migration during cerebellar development. In the present report we test the prediction that mutant and transgenic mouse models on a C57BL/6 background will also exhibit these same cerebellar malformations. Consistent with our hypothesis, we found that 2 spontaneous mutant models of Parkinson's disease on a C57BL/6 background had cerebellar malformations. In addition, we found that numerous transgenic mouse lines on a full or partial C57BL/6 background including eGFP-, YFP- and Cre-transgenic mice also exhibited heterotopia. These data suggest that histological analyses be performed in studies of cerebellar function or development when using C57BL/6 or other mice on this background in order for correct interpretation of research results.
authors.affiliation: Department of Biomedical Sciences, New York Institute of Technology, College of Osteopathic Medicine, Old Westbury, NY 11568-8000, USA. rramos02@nyit.edu
authors.forename: Raddy L
authors.initials: RL
authors.lastname: Ramos
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Van Dine
authors.forename: Elizabeth
authors.initials: E
authors.lastname: George
authors.forename: Dhruv
authors.initials: D
authors.lastname: Patel
authors.forename: Blair J
authors.initials: BJ
authors.lastname: Hoplight
authors.forename: Joerg R
authors.initials: JR
authors.lastname: Leheste
authors.forename: Eric K
authors.initials: EK
authors.lastname: Richfield
authors.forename: German
authors.initials: G
authors.lastname: Torres
mesh_terms: D000818:Animals; D002531:Cerebellum / Q000002:abnormalities* / Q000473:pathology; D005260:Female; D008297:Male; D054081:Malformations of Cortical Development, Group II / Q000235:genetics* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D008817:Mice, Mutant Strains; D008822:Mice, Transgenic
keywords: Cerebellar dysplasia; Foliation; Heterotopia; Lamination; Malformation; Mouse models