title: [Structure and dynamics of microbial community in the denitrifying sulfide removal process].
abstract: In order to investigate the relationship between the structure of microbial community and the operational efficiency of the denitrifying sulfide removal (DSR) process, whole genomic DNA was extracted from sludge samples at each operational stage. Functional gene arrays (FGAs), a high throughput metagenomic technology, was employed to characterize the change of microbial community structure in DSR process. The results of diversity indices (Simpson and Shannon indices) and clustering showed that the microbial community structure changed greatly with stages according to the operational conditions. In stage I and II, diversity indices and richness decreased due to acclimation of the microbial community to the environment, whereas they increased notably according to the maturing of sludge which may indicate that the bioreactor entered the steady stage. Analysis of key functional genes showed that the abundance of genes did not only reflect the activities of functional bacteria but also had a close relationship with the reactor performance.
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China micro_yh@126.com
authors.forename: Hao
authors.initials: H
authors.lastname: Yu
authors.forename: Ai-Jie
authors.initials: AJ
authors.lastname: Wang
authors.forename: Chuan
authors.initials: C
authors.lastname: Chen
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D058440:Denitrification; D007220:Industrial Waste; D013440:Sulfides / Q000302:isolation & purification* / Q000378:metabolism; D014865:Waste Disposal, Fluid / Q000379:methods*; D062065:Wastewater / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D007220:Industrial Waste; D013440:Sulfides; D062065:Wastewater