title: Effect on emotional behavior and stress by inhalation of the essential oil from Chamaecyparis obtusa.
abstract: Various effects have been reported in the literature for the essential oil from Chamaecyparis obtusa (EOCO), such as antibacterial and antifungal activity. In this study, we examined the effect of EOCO on emotional behavior and stress-induced biomarkers. Male ICR mice, aged 5 weeks at the start of each experiment, were individually housed in cages for 1 week. After placing each mouse in a glass container and exposing it to EOCO for 90 min, we then investigated the influence on emotional behavior using the elevated-plus maze (EPM) test, which is one of the evaluation methods for anxiolytic-like behavior. Significant anxiolytic-like effects were observed for the 7.0 mg/L air EOCO (P < 0.05). After the EPM test, mice were dissected and changes in the stress-induced biomarkers within the brain were investigated by examining the amounts of fast nerve growth factor receptor (NGFR) and activity regulated cytoskeletal-associated protein (Arc) gene expression, and brain-derived neurotrophic factor (BDNF) and galactokinase 1 (GLK1) protein expression. Significant increases were observed in the amount of NGFR after inhalation of 7.0 mg/L air EOCO (P < 0.05). These results indicate that EOCO has both anxiolytic-like and stress mitigation effects.
authors.affiliation: Faculty of Pharmaceutical Sciences, Toho University, Funabashi, Chiba 274-8510, Japan.
authors.forename: Hikaru
authors.initials: H
authors.lastname: Kasuya
authors.forename: Erika
authors.initials: E
authors.lastname: Hata
authors.forename: Tadaaki
authors.initials: T
authors.lastname: Satou
authors.forename: Masaki
authors.initials: M
authors.lastname: Yoshikawa
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Hayashi
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Masuo
authors.forename: Kazuo
authors.initials: K
authors.lastname: Koike
mesh_terms: D000280:Administration, Inhalation; D000818:Animals; D014151:Anti-Anxiety Agents / Q000494:pharmacology*; D029780:Chamaecyparis / Q000737:chemistry*; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D051379:Mice; D008813:Mice, Inbred ICR; D009822:Oils, Volatile / Q000494:pharmacology*; D017475:Receptors, Nerve Growth Factor / Q000032:analysis; D013315:Stress, Psychological / Q000378:metabolism*
chemical_list: D014151:Anti-Anxiety Agents; D009822:Oils, Volatile; D017475:Receptors, Nerve Growth Factor