title: Increased osteopontin expression in HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patient cells is associated with IL-17 expression.
abstract: BACKGROUND
  HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) is a neurological inflammatory disease associated with a predominant infiltration of CD4+ T lymphocytes, which are the main subset of HTLV-1-infected cells. It has been demonstrated that in cell line the viral Tax protein transcriptionnally regulate expression of osteopontin, an inflammatory cytokine associated with Th17-related pathologies.
  
  
  OBJECTIVE
  The aim of the study was to explore osteopontin expression in HTLV-1 asymptomatic carriers and in HAM/TSP patients and consequences on IL17 expression.
  
  
  METHODS
  We quantified Tax, osteopontin, RORγ, IL17 and IL22 mRNA expressions in cells from 10 HAM/TSP patients, 6 asymptomatic HTLV-1 carriers (ASY) and 4 HTLV-1-negative healthy donors during ex vivo culture.
  
  
  RESULTS
  We observed that the expression of osteopontin was higher in HAM/TSP patients and correlated with Tax expression levels. Positive regulation of RORγ, IL17 and IL22 were also observed during cell culture.
  
  
  CONCLUSIONS
  Our results propose a new mechanism which could contribute to HAM/TSP pathogenesis.
authors.affiliation: CNRS, Centre d'études d'agents Pathogènes et Biotechnologies pour la Santé (CPBS), UMR 5236, F-34293 Montpellier, France.
authors.forename: Sarkis
authors.initials: S
authors.lastname: Sarkis
authors.forename: Gildas
authors.initials: G
authors.lastname: Belrose
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Peloponese
authors.forename: Stéphane
authors.initials: S
authors.lastname: Olindo
authors.forename: Raymond
authors.initials: R
authors.lastname: Césaire
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Mesnard
authors.forename: Antoine
authors.initials: A
authors.lastname: Gross
mesh_terms: D000368:Aged; D058070:Asymptomatic Diseases; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002353:Carrier State / Q000276:immunology / Q000473:pathology*; D002478:Cells, Cultured; D005260:Female; D020869:Gene Expression Profiling; D016356:Gene Products, tax / Q000096:biosynthesis; D015368:Human T-lymphotropic virus 1 / Q000276:immunology*; D006801:Humans; D020381:Interleukin-17 / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D053495:Osteopontin / Q000096:biosynthesis*; D015493:Paraparesis, Tropical Spastic / Q000276:immunology / Q000473:pathology*
chemical_list: D016356:Gene Products, tax; D020381:Interleukin-17; C514970:tax protein, Human T-lymphotrophic virus 1; D053495:Osteopontin
keywords: HAM/TSP; HTLV-1; IL-17; Osteopontin