title: Synthesis of oligonucleotides with glucosamine at the 3'-position and evaluation of their biological activity.
abstract: Short interfering RNA (siRNA) has been proven to be an utilizable tool for post-transcriptional gene silencing research. In this study, we designed and synthesized two glucosamine analogues and tried to modify the siRNA using these two glucosamine analogues at the 3'-overhang region of siRNAs to improve the nuclease resistance and to overcome some other weak points. The siRNAs modified with glucosamine analogues had almost no effect of the thermal stability and showed strong resistance to nuclease degradation. Some of them kept the same gene silencing activity level as unmodified siRNA.
authors.affiliation: United Graduate School of Drug Discovery and Medical Information Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: Xiong
authors.initials: X
authors.lastname: Luo
authors.forename: Takahiro
authors.initials: T
authors.lastname: Sugiura
authors.forename: Remi
authors.initials: R
authors.lastname: Nakashima
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kitamura
authors.forename: Yukio
authors.initials: Y
authors.lastname: Kitade
mesh_terms: D000818:Animals; D005092:Exonucleases / Q000378:metabolism; D005944:Glucosamine / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D049410:Luciferases, Renilla / Q000235:genetics / Q000378:metabolism; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000138:chemical synthesis* / Q000378:metabolism; D048790:Renilla / Q000201:enzymology; D012910:Snake Venoms / Q000201:enzymology; D044366:Transition Temperature
chemical_list: D009841:Oligonucleotides; D034741:RNA, Small Interfering; D012910:Snake Venoms; D049410:Luciferases, Renilla; D005092:Exonucleases; C022650:spleen exonuclease; D010727:Phosphoric Diester Hydrolases; D005944:Glucosamine