title: A liquid chromatography-tandem mass spectrometric method for the simultaneous quantitation of five components of Ixeris sonchifoliain (Bge.) Hance in rat plasma and its application to a pharmacokinetic study.
abstract: A rapid and sensitive liquid chromatography-tandem mass spectrometric (LC-MS/MS) method for the simultaneous quantitation of five major active ingredients of Ixeris sonchifolia (Bge.) Hance in rat plasma has been developed and validated. After liquid-liquid extraction of 50μL plasma with ethyl acetate, analytes and internal standard (I.S.), astilbin, were chromatographed on a Zorbax SB-C18 column (150mm×4.6mm, 5μm) using acetonitrile - 10mM ammonium acetate (60:40, v/v, pH 5.6) as mobile phase. The five analytes: chicoric acid, luteolin 7-O-β-d-glucuronide, luteolin 7-O-β-d-glucopyranoside, luteolin 7-O-β-d-glucopyranosyl-(1→2)-β-d-glucopyranoside, apigenin 7-O-β-d-glucuronide and I.S., were detected by negative ion electrospray ionization followed by multiple reaction monitoring of the ions with m/z 473.0→311.0, 461.0→285.0, 447.0→285.0, 609.1→285.0, 445.1→269.0 and 449.1→150.9, respectively. The method was linear for all analytes in the concentration range 10-3000ng/mL with intra- and inter-day precision (as relative standard deviation) ≤8.99% and accuracy (as relative error) ≤4.00%. The limits of detection (LOD) were 5, 1, 5, 5, 2ng/mL for chicoric acid, luteolin 7-O-β-d-glucuronide, luteolin 7-O-β-d-glucopyranoside, luteolin 7-O-β-d-glucopyranosyl-(1→2)-β-d-glucopyranoside, apigenin 7-O-β-d-glucuronide, respectively. The method was successfully applied to a pharmacokinetic study of the five analytes in rat after a single intravenous dose of Kudiezi Injection.
authors.affiliation: Clinical Pharmacology Center, Research Institute of Translational Medicine, The First Hospital of Jilin University, Dongminzhu Street, Changchun 130061, PR China; College of Life Science, Jilin University, Qianjin Street, Changchun 130012, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yin
authors.forename: Meiyun
authors.initials: M
authors.lastname: Shi
authors.forename: Yantong
authors.initials: Y
authors.lastname: Sun
authors.forename: Xiaocheng
authors.initials: X
authors.lastname: Sun
authors.forename: Huan
authors.initials: H
authors.lastname: Meng
authors.forename: J Paul
authors.initials: JP
authors.lastname: Fawcett
authors.forename: Yan
authors.initials: Y
authors.lastname: Yang
authors.forename: Jingkai
authors.initials: J
authors.lastname: Gu
mesh_terms: D000818:Animals; D019659:Asteraceae / Q000737:chemistry*; D002109:Caffeic Acids / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D002853:Chromatography, Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000493:pharmacokinetics*; D005260:Female; D005419:Flavonoids / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D020719:Glucuronides / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D016018:Least-Squares Analysis; D008297:Male; D010936:Plant Extracts / Q000737:chemistry* / Q000493:pharmacokinetics*; D051381:Rats; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013386:Succinates / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D002109:Caffeic Acids; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D020719:Glucuronides; D010936:Plant Extracts; D013386:Succinates; C100435:chicoric acid