title: Effects of glutamine on wound healing.
abstract: Studies reporting the need for replacing amino acids such as glutamine (Gln), hydroxymethyl butyrate (HMB) and arginine (Arg) to accelerate wound healing are available in the literature. The primary objective of this study was to present the effects of Gln on tissue hydroxyproline (OHP) levels in wound healing. This study was conducted on 30 female Sprague Dawley rats with a mean weight of 230 ± 20 g. Secondary wounds were formed by excising 2 × 1 cm skin subcutaneous tissue on the back of the rats. The rats were divided into three equal groups. Group C (Control): the group received 1 ml/day isotonic solution by gastric gavage after secondary wound was formed. Group A (Abound): the group received 0·3 g/kg/day/ml Gln, 0·052 g/kg/day/ml HMB and 0·3 g/kg/day/ml Arg by gastric gavage after secondary wound was formed. Group R (Resource): the group received 0·3 g/kg/day/ml Gln by gastric gavage after secondary wound was formed. The OHP levels of the tissues obtained from the upper half region on the 8th day and the lower half region on the 21st day from the same rats in the groups were examined. Statistical analysis was performed using the statistics program SPSS version 17.0. No statistically significant differences were reported with regard to the OHP measurements on the 8th and 21st days (8th day: F = 0·068, P = 0·935 > 0·05; 21st day: F = 0·018, P = 0·983 > 0·05). The increase in mean OHP levels on the 8th and 21st days within each group was found to be statistically significant (F = 1146·34, P = 0·000 < 0·001). We conclude that in adults who eat healthy food, who do not have any factor that can affect wound healing negatively and who do not have large tissue loss at critical level, Gln, Arg and HMB support would not be required to accelerate secondary wound healing.
authors.affiliation: Department of General Surgery, Akcaabat Hackali Baba State Hospital, Trabzon, Turkey.
authors.forename: Ugur
authors.initials: U
authors.lastname: Kesici
authors.affiliation: Department of Anesthesiology and Reanimation, Kanuni Training and Research Hospital, Trabzon, Turkey.
authors.forename: Sevgi
authors.initials: S
authors.lastname: Kesici
authors.affiliation: Department of Anesthesiology and Reanimation, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey.
authors.forename: Hulya
authors.initials: H
authors.lastname: Ulusoy
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey.
authors.forename: Fulya
authors.initials: F
authors.lastname: Yucesan
authors.affiliation: Department of Anesthesiology and Reanimation, Okmeydani Training and Research Hospital, Istanbul, Turkey.
authors.forename: Aygen U
authors.initials: AU
authors.lastname: Turkmen
authors.affiliation: Department of Anesthesiology and Reanimation, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Besir
authors.affiliation: Department of Anesthesiology and Reanimation, Kanuni Training and Research Hospital, Trabzon, Turkey.
authors.forename: Verda
authors.initials: V
authors.lastname: Tuna
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D005973:Glutamine / Q000494:pharmacology*; D011379:Prognosis; D051381:Rats; D017207:Rats, Sprague-Dawley; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000628:therapy*
chemical_list: D005973:Glutamine
keywords: Arginine; Glutamine; Hydroxymethyl butyrate; Wound healing