title: MiRNA-199a-3p: A potential circulating diagnostic biomarker for early gastric cancer.
abstract: BACKGROUND
  Tumor-associated miRNAs have been detected in serum or plasma. We investigated whether plasma miRNA-199a-3p could be a potential circulating biomarker for early gastric cancer (EGC).
  
  
  METHODS
  By using real-time qRT-PCR, the expression of miRNA-199a-3p were compared between these pre-operative plasmas from 30 EGC patients and 70 healthy controls, and between these pre-operative and post-operative plasmas. Further validation was on an independent set of plasmas from 50 EGC patients.
  
  
  RESULTS
  The expression of miRNA-199a-3p (47.5 ± 6.5) in plasma in EGC patients was significantly higher than that from healthy controls (13.9 ± 2.7, P < 0.001) and gastric precancerous diseases (GPD) patients (19.2 ± 2.5, P = 0.004), respectively. Furthermore, the expression levels of miRNA-199a-3p (11.8 ± 2.9, P = 0.012) in the post-operative plasmas were significantly reduced when compared to the pre-operative plasmas. With respect of clinicopathological characteristics, the expression of miRNA-199a-3p in plasma was not associated with the depth of tumor invasion. Moreover, the AUC of the expression of miRNA-199a-3p in plasma for EGC diagnosis was 0.818, which was significantly higher than that of combined tumor markers (0.556). The sensitivity, specificity and accuracy of miRNA-199a-3p expression in plasma for EGC diagnosis were 76%, 74%, and 75%, respectively.
  
  
  CONCLUSIONS
  Plasma miRNA-199a-3p could be a novel potential diagnostic biomarkers for EGC.
authors.affiliation: Department of Surgery, Shanghai Key Laboratory of Gastric Neoplasms, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, PR China.
authors.forename: Chen
authors.initials: C
authors.lastname: Li
authors.forename: Jian Fang
authors.initials: JF
authors.lastname: Li
authors.forename: Qu
authors.initials: Q
authors.lastname: Cai
authors.forename: Qing Qing
authors.initials: QQ
authors.lastname: Qiu
authors.forename: Min
authors.initials: M
authors.lastname: Yan
authors.forename: Bing Ya
authors.initials: BY
authors.lastname: Liu
authors.forename: Zheng Gang
authors.initials: ZG
authors.lastname: Zhu
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood*; D016022:Case-Control Studies; D005260:Female; D005743:Gastrectomy; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D008875:Middle Aged; D009367:Neoplasm Staging; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013274:Stomach Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000473:pathology / Q000601:surgery; D016896:Treatment Outcome
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; C531408:mirn199 microRNA, human
keywords: biomarker; diagnosis; early gastric cancer; microRNA