title: Adduct of malondialdehyde to hemoglobin: a new marker of oxidative stress that is associated with significant morbidity in preterm infants.
abstract: Preterm infants (PT) are particularly exposed to oxidative stress (OS), and a blood-sparing marker, the malondialdehyde adduct to hemoglobin (MDA-Hb), may be useful to accurately assess OS-related neonatal morbidity. In a prospective study, MDA-Hb concentrations were assessed in two groups of PT, one with and one without severe neonatal morbidity as estimated by a composite index of severe morbidity (ISM). All PT born in a single tertiary care NICU (<32 weeks and birth weight <1500 g) were consecutively included. MDA-Hb and blood glutathione (GSH) concentrations were measured by liquid chromatography-mass spectrometry during the first 6 weeks of life. Linear regressions and a multilevel model were fitted to study the relationship between MDA-Hb or GSH and ISM. Of the 83 PT (mean ± SD: 28.3 ± 2 weeks, 1089 ± 288 g), 21% presented severe neonatal morbidity. In the multivariate model, MDA-Hb concentrations were significantly higher in the ISM+ group than in the ISM- group during the first 6 weeks of life (P = 0.009). No significant difference in GSH concentrations was observed between groups (P = 0.180). MDA-Hb is a marker of interest for estimating oxidative stress in PT and could be useful to evaluate the impact of strategies to improve perinatal outcomes.
authors.affiliation: Département de Néonatologie, CHU Angers, 49100 Angers, France. cecile.cipierre@wanadoo.fr
authors.forename: Cécile
authors.initials: C
authors.lastname: Cipierre
authors.forename: Stéphane
authors.initials: S
authors.lastname: Haÿs
authors.forename: Delphine
authors.initials: D
authors.lastname: Maucort-Boulch
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Steghens
authors.forename: Jean-Charles
authors.initials: JC
authors.lastname: Picaud
mesh_terms: D015415:Biomarkers / Q000378:metabolism*; D005260:Female; D005978:Glutathione / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature / Q000378:metabolism*; D019102:Infant, Very Low Birth Weight; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism*; D018384:Oxidative Stress; D047928:Premature Birth / Q000453:epidemiology* / Q000473:pathology*
chemical_list: D015415:Biomarkers; D006454:Hemoglobins; D008315:Malondialdehyde; D005978:Glutathione