title: Association between genetic variants in pre-miRNA and colorectal cancer risk in a Chinese population.
abstract: BACKGROUND
  Single-nucleotide polymorphisms (SNPs) in pre-miRNAs may alter microRNA expression levels or processing and then contribute to the susceptibility of cancer development. We hypothesized that SNPs in pre-miRNAs may be associated with the risk of colorectal cancer (CRC).
  
  
  METHODS
  We genotyped four common polymorphisms (i.e., rs11614913, rs3746444, rs2910164, and rs2292832) in pre-miRNAs of 353 CRC patients and 540 healthy controls to investigate the association between the SNPs and the risk of CRC using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay.
  
  
  RESULTS
  The rs11614913 CT, TT genotypes, and T allele were associated with an increased risk of CRC compared with the CC genotype and C allele (CT vs. CC: OR = 7.34, 95% CI 3.76-14.34; TT vs. CC: OR = 13.66, 95% CI 6.76-27.6; T vs. C: OR = 1.99, 95% CI 1.63-2.42, respectively). Interestingly, using the rs2910164 GG genotype as a reference, the rs2910164 GC genotype was associated with an increased risk of CRC (OR = 1.49, 95% CI 1.02-2.18), whereas the rs2910164 CC genotype was associated with a decreased risk of CRC (OR = 0.58, 95% CI 0.37-0.93). When compared with the rs2910164G allele, rs2910164 C allele was associated with a reduced risk of CRC (OR = 0.80, 95% CI 0.66-0.97, p = 0.02).
  
  
  CONCLUSIONS
  These findings suggest that rs11614913 and rs2910164 polymorphisms may be associated with the etiology of CRC.
authors.affiliation: Department of Immunology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, Sichuan, People's Republic of China.
authors.forename: Meili
authors.initials: M
authors.lastname: Lv
authors.forename: Wei
authors.initials: W
authors.lastname: Dong
authors.forename: Lijuan
authors.initials: L
authors.lastname: Li
authors.forename: Lushun
authors.initials: L
authors.lastname: Zhang
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Su
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Linbo
authors.initials: L
authors.lastname: Gao
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
mesh_terms: D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors
chemical_list: D035683:MicroRNAs