title: [Molecular basis and clinical blood transfusion for cases with ABO typing discrepancy].
abstract: OBJECTIVE
  To investigate the molecular genetic basis of samples with ABO typing discrepancy and provide the guidline for identification and clinical transfusion for these samples.
  
  
  METHODS
  Six cases with similar serological characteristics were collected. Serological method, PCR-SSP and direct sequencing of ABO gene were used to explore the underlying mechanism. Condition of clinical transfusion of patients was also reviewed.
  
  
  RESULTS
  Three conditions were related with the ABO blood type discrepancy, which included weaken antigen (2 cases), weakened antibody (3 cases) and ABO subtype (1 case). The satisfactory effect of transfusion was achieved in all patients with the principle of the same blood type or the compatible crossmatch.
  
  
  CONCLUSIONS
  Heterogeneity has existed with the ABO group. Indivianals with same reaction pattern may result in different mechanisms.
authors.affiliation: Ningbo Central Blood Station, Ningbo, Zhejiang 315040, P.R. China. dgflying@gmail.com
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhang
authors.forename: Gang
authors.initials: G
authors.lastname: Deng
authors.forename: Dandan
authors.initials: D
authors.lastname: Huang
authors.forename: Wenyu
authors.initials: W
authors.lastname: Guo
authors.forename: Deyi
authors.initials: D
authors.lastname: Xu
authors.forename: Yong
authors.initials: Y
authors.lastname: Du
authors.forename: Xiaoxian
authors.initials: X
authors.lastname: Tang
authors.forename: Yong
authors.initials: Y
authors.lastname: Yu
authors.forename: Danfei
authors.initials: D
authors.lastname: Deng
mesh_terms: D000017:ABO Blood-Group System / Q000235:genetics*; D000328:Adult; D001483:Base Sequence; D001788:Blood Grouping and Crossmatching; D001803:Blood Transfusion; D005091:Exons; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D055815:Young Adult
chemical_list: D000017:ABO Blood-Group System