title: Multigene engineering of starch biosynthesis in maize endosperm increases the total starch content and the proportion of amylose.
abstract: Maize (Zea mays spp. mays) is a staple crop for more than 900 million people. The seeds or kernels provide a rich source of calories because ~70% of the weight is carbohydrate, mostly in the form of starch. The content and composition of starch are complex traits controlled by many genes, offering multiple potential targets for intervention. We used a multigene engineering approach combining the overexpression of Bt2, Sh2, Sh1 and GbssIIa (to enhance the activity of sucrose synthase, AGPase and granule-bound starch synthase) with the suppression of SbeI and SbeIIb by RNA interference (to reduce the activity of starch branching enzyme). Maize plants expressing all six genes plus the selectable marker showed a 2.8-7.7% increase in the endosperm starch content and a 37.8-43.7% increase in the proportion of amylose, which was significant compared to untransformed control plants. We also observed improvements in other agronomic traits, such as a 20.1-34.7% increase in 100-grain weight, a 13.9-19.0% increase in ear weight, and larger kernels with a better appearance, presumably reflecting the modified starch structure within the kernels. Our results confirm that multigene engineering applied to the starch biosynthesis pathway can not only modulate the quality and quantity of starch but can also improve starch-dependent agronomic traits.
authors.affiliation: Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University, Changchun, 130024, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Jiang
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Yu
authors.forename: Xin
authors.initials: X
authors.lastname: Qi
authors.forename: Qian
authors.initials: Q
authors.lastname: Yu
authors.forename: Sen
authors.initials: S
authors.lastname: Deng
authors.forename: Bing
authors.initials: B
authors.lastname: Bai
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Ai
authors.initials: A
authors.lastname: Zhang
authors.forename: Changfu
authors.initials: C
authors.lastname: Zhu
authors.forename: Bao
authors.initials: B
authors.lastname: Liu
authors.forename: Jinsong
authors.initials: J
authors.lastname: Pang
mesh_terms: D015061:1,4-alpha-Glucan Branching Enzyme / Q000096:biosynthesis / Q000235:genetics; D000688:Amylose / Q000737:chemistry / Q000235:genetics*; D056625:Endosperm / Q000737:chemistry / Q000235:genetics* / Q000254:growth & development; D018506:Gene Expression Regulation, Plant; D005818:Genetic Engineering; D005964:Glucosyltransferases / Q000096:biosynthesis; D010940:Plant Proteins / Q000235:genetics; D034622:RNA Interference; D013213:Starch / Q000096:biosynthesis* / Q000737:chemistry; D013214:Starch Synthase / Q000096:biosynthesis / Q000235:genetics; D013395:Sucrose / Q000378:metabolism; D003313:Zea mays / Q000737:chemistry / Q000235:genetics*
chemical_list: D010940:Plant Proteins; C111076:Sh2 protein, plant; D013395:Sucrose; D013213:Starch; D000688:Amylose; D005964:Glucosyltransferases; C022699:sucrose synthase; D015061:1,4-alpha-Glucan Branching Enzyme; D013214:Starch Synthase