title: High expression of lysine-specific demethylase 1 correlates with poor prognosis of patients with esophageal squamous cell carcinoma.
abstract: Recent studies have elucidated the role of lysine-specific demethylase 1 (LSD1), a member of the histone demethylases, in epigenetic regulation of tumor suppressing/promoting genes and neoplastic growth. However, the expression of LSD1 in patients with esophageal squamous cell carcinoma (ESCC) is still unknown. Here, we reported that LSD1 expression was elevated in cancerous tissue and correlated with lymph node metastasis and poorer overall survival in patients with ESCC. Compared to EC109 cells, LSD1 expression was unregulated in aggressive cancer cell lines KYSE450 and KYSE150. Knockdown of LSD1 using lentivirus delivery of LSD1-specific shRNA abrogated the migration and invasion of ESCC cells in vitro. Further, a LSD1 inhibitor, tranylcypromine, suppressed H3K4me2 demethylation and attenuated cellular motility and invasiveness in a dose-dependent manner. Taken together, these data suggested that LSD1 was a potential prognostic maker and may be a molecular target for inhibiting invasion and metastasis in ESCC.
authors.affiliation: Department of Gastroenterology, Institute of Surgery Research, Daping Hospital, The Third Military Medical University, Chongqing 400042, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Yu
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.forename: Kun
authors.initials: K
authors.lastname: Zhang
authors.forename: Zengjie
authors.initials: Z
authors.lastname: Lei
authors.forename: Yan
authors.initials: Y
authors.lastname: Guo
authors.forename: Hualiang
authors.initials: H
authors.lastname: Xiao
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Lilin
authors.initials: L
authors.lastname: Fan
authors.forename: Chunhui
authors.initials: C
authors.lastname: Lan
authors.forename: Yanling
authors.initials: Y
authors.lastname: Wei
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ma
authors.forename: Li
authors.initials: L
authors.lastname: Lin
authors.forename: Chengyi
authors.initials: C
authors.lastname: Mao
authors.forename: Xin
authors.initials: X
authors.lastname: Yang
authors.forename: Xiaodi
authors.initials: X
authors.lastname: Chen
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Yun
authors.initials: Y
authors.lastname: Bai
authors.forename: Dongfeng
authors.initials: D
authors.lastname: Chen
mesh_terms: D001483:Base Sequence; D002294:Carcinoma, Squamous Cell / Q000201:enzymology* / Q000473:pathology; D017931:DNA Primers; D004938:Esophageal Neoplasms / Q000201:enzymology* / Q000473:pathology; D005260:Female; D056466:Histone Demethylases / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D056466:Histone Demethylases; C495195:KDM1A protein, human
keywords: Esophageal squamous cell carcinoma; Invasion; LSD1