title: Analysis of pre-mRNA splicing using HeLa cell nuclear extracts.
abstract: This protocol is used to determine the splicing behavior of pre-mRNAs in cell extracts that are capable of carrying out splicing (e.g., nuclear extracts from HeLa cells). (32)P-labeled RNA is incubated under splicing conditions for various times, and the resulting products are analyzed on denaturing polyacrylamide gels.
authors.forename: Timothy W
authors.initials: TW
authors.lastname: Nilsen
mesh_terms: D002457:Cell Extracts / Q000302:isolation & purification*; D002467:Cell Nucleus; D003584:Cytological Techniques / Q000379:methods*; D058645:Denaturing Gradient Gel Electrophoresis; D006367:HeLa Cells; D006801:Humans; D007553:Isotope Labeling; D010761:Phosphorus Radioisotopes / Q000378:metabolism; D012322:RNA Precursors / Q000378:metabolism*; D012326:RNA Splicing
chemical_list: D002457:Cell Extracts; D010761:Phosphorus Radioisotopes; D012322:RNA Precursors