title: Multiple-locus variable-number tandem-repeat analysis of mycoplasma pneumoniae clinical specimens and proposal for amendment of MLVA nomenclature.
abstract: Mycoplasma pneumoniae is one of the major respiratory bacterial pathogens that cause pneumonia in humans. Multiple-locus variable-number tandem-repeat analysis (MLVA) is currently the most discriminative method for typing M. pneumoniae strains. To better understand the epidemic of M. pneumoniae-related pneumonia in pediatric patients in Beijing, China, we performed MLVA analysis on 118 specimens collected during an epidemic from 2010-2012. Eleven distinct MLVA types were identified, including four novel types. There was no obvious association of macrolide resistance with any of the genotypes. Considering the instability of VNTR locus Mpn1, we propose an amended MLVA nomenclature system based on the remaining four VNTR loci.
authors.affiliation: Department of Bacteriology, Capital Institute of Pediatrics, Chaoyang District, Beijing, China. s.hongmei@263.net
authors.forename: Hongmei
authors.initials: H
authors.lastname: Sun
authors.forename: Guanhua
authors.initials: G
authors.lastname: Xue
authors.forename: Chao
authors.initials: C
authors.lastname: Yan
authors.forename: Shaoli
authors.initials: S
authors.lastname: Li
authors.forename: Ling
authors.initials: L
authors.lastname: Cao
authors.forename: Yi
authors.initials: Y
authors.lastname: Yuan
authors.forename: Hanqing
authors.initials: H
authors.lastname: Zhao
authors.forename: Yanling
authors.initials: Y
authors.lastname: Feng
authors.forename: Liqiong
authors.initials: L
authors.lastname: Wang
authors.forename: Zhaoyang
authors.initials: Z
authors.lastname: Fan
mesh_terms: D002648:Child; D002675:Child, Preschool; D024881:Drug Resistance, Bacterial / Q000235:genetics; D005260:Female; D005798:Genes, Bacterial / Q000235:genetics; D056426:Genetic Loci / Q000235:genetics*; D006801:Humans; D007223:Infant; D018942:Macrolides / Q000494:pharmacology; D008297:Male; D009154:Mutation; D009177:Mycoplasma pneumoniae / Q000145:classification* / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D012150:Polymorphism, Restriction Fragment Length; D012329:RNA, Bacterial / Q000235:genetics; D012335:RNA, Ribosomal / Q000235:genetics; D020080:Tandem Repeat Sequences / Q000235:genetics*; D009626:Terminology as Topic
chemical_list: D018942:Macrolides; D012329:RNA, Bacterial; D012335:RNA, Ribosomal