title: Laser capture microdissection shows HPV11 as both a causal and a coincidental infection in cervical cancer specimens with multiple HPV types.
abstract: OBJECTIVE
  To identify, by laser capture microdissection (LCM), the cellular localization of HPV11 when present with carcinogenic HPV in invasive cervical cancer (ICC) specimens, and to relate this to p16(INK) (4a) expression.
  
  
  RESULTS
  Three squamous cell ICC specimens showing coinfection with HPV11 and carcinogenic HPV16 or HPV31 were selected from the Institut Català d'Oncologia international survey of anogenital carcinomas, and coinfection was confirmed by SPF10 -DEIA-LiPA25 analysis. In two cases LCM-PCR identified HPV11 in low-grade and high-grade squamous intraepithelial lesions (SILs) adjacent to the ICC, and HPV16 or HPV31 in the ICC. In one case, HPV11 was the only genotype found in the ICC. P16(INK) (4a) expression was diffuse in ICC associated with carcinogenic HPV, but focal in ICC with HPV11.
  
  
  CONCLUSIONS
  Our results confirm that a single cervical, cancerous or precancerous lesion is associated with a single HPV type. Detecting low-risk HPV as a coinfection in whole tissue from ICC does not prove a causal association. HPV11 may be found only in an adjacent SIL with carcinogenic HPV in the ICC. It is also found alone in carcinoma. LCM-PCR and differential P16(INK) (4a) expression can clarify the causal role of each type when multiple HPVs are present in whole tissue from carcinomas.
authors.affiliation: DDL Diagnostic Laboratory, Rijswijk, The Netherlands. Nuria.Guimera@ddl.nl
authors.forename: Núria
authors.initials: N
authors.lastname: Guimerà
authors.forename: Belen
authors.initials: B
authors.lastname: Lloveras
authors.forename: Laia
authors.initials: L
authors.lastname: Alemany
authors.forename: Ermina
authors.initials: E
authors.lastname: Iljazovic
authors.forename: Hai-Rim
authors.initials: HR
authors.lastname: Shin
authors.forename: Suh
authors.initials: S
authors.lastname: Jung-Il
authors.forename: Silvia
authors.initials: S
authors.lastname: de Sanjose
authors.forename: David
authors.initials: D
authors.lastname: Jenkins
authors.forename: F Xavier
authors.initials: FX
authors.lastname: Bosch
authors.forename: Wim
authors.initials: W
authors.lastname: Quint
mesh_terms: D000328:Adult; D060085:Coinfection / Q000378:metabolism / Q000473:pathology / Q000821:virology; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000378:metabolism; D005260:Female; D052140:Human papillomavirus 11 / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity*; D052162:Human papillomavirus 16 / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D058952:Human papillomavirus 31 / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D006801:Humans; D061346:Laser Capture Microdissection; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D002583:Uterine Cervical Neoplasms / Q000378:metabolism / Q000473:pathology / Q000821:virology*
chemical_list: D019941:Cyclin-Dependent Kinase Inhibitor p16
keywords: HPV11; low-risk HPV; multiple HPV infections