title: Large-scale preparation of Asn-glycopeptide carrying structurally homologous antigenic N-glycan.
abstract: In our previous report (M. Okano, et al., Clin. Exp. Allergy, 34, 770-778 (2004)), we found that free plant complex type N-glycans suppressed the production of IL4 from Th2 cells of Japanese cedar pollinosis patients, suggesting that plant complex type N-glycan can be used as a leading compound for developing immuno-pharmaceuticals. Although immunoreactive plant complex type N-glycans occur ubiquitously on glycoproteins expressed in plants, an appropriate procedure has not been established to prepare non-labeled immunoreactive glycans or glycopeptides bearing structurally homologous immunoreactive glycans in large amounts. In this study, therefore, we developed a new preparative procedure for the large-scale preparation of Asn-glycopeptide bearing plant complex type N-glycan using a combination of gel-filtration and the hydrophilic partitioning method. By this new method, about 103 mg of Asn-glycopeptide bearing the antigenic N-glycans was obtained from 1.9 kg of shelled Ginkgo biloba seeds.
authors.affiliation: Department of Biofunctional Chemistry, Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan. mmaeda@cc.okayama-u.ac.jp
authors.forename: Megumi
authors.initials: M
authors.lastname: Maeda
authors.forename: Naoto
authors.initials: N
authors.lastname: Takeda
authors.forename: Aya
authors.initials: A
authors.lastname: Mano
authors.forename: Maomi
authors.initials: M
authors.lastname: Yamanishi
authors.forename: Mariko
authors.initials: M
authors.lastname: Kimura
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Kimura
mesh_terms: D000941:Antigens / Q000737:chemistry / Q000276:immunology*; D001216:Asparagine / Q000737:chemistry* / Q000276:immunology; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D020441:Ginkgo biloba / Q000737:chemistry / Q000276:immunology; D006020:Glycopeptides / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D011134:Polysaccharides / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism*; D012639:Seeds / Q000737:chemistry / Q000276:immunology
chemical_list: D000941:Antigens; D006020:Glycopeptides; D011134:Polysaccharides; D001216:Asparagine