title: [Influence of recombination abnormality in spermatocyte meiosis on the pathology of patients with non-obstructive azoospermia].
abstract: OBJECTIVE
  To primarily study the influence of recombination abnormality in human spermatocyte meiosis on the pathology of the patients with non-obstructive azoospermia (NOA).
  
  
  METHODS
  We obtained testis tissues from 6 NOA patients by testicular biopsy and divided the tissue of each patient into 2 portions, one for pathological examination and the other for immunofluorescent staining. We observed the synaptonemal complex and the numbers of the recombination sites on homologous chromosomes, and analyzed the relationship between abnormal recombination and pathological findings.
  
  
  RESULTS
  Pathological examination showed that the basement membrane of the seminiferous tubules was thickened in 3 of the cases and atrophied in the other 3, the number of autosomal MLH1 foci in a spermatocyte ranging from 10 to 50 in the former 3, and from 30 to 50 in the latter 3.
  
  
  CONCLUSIONS
  The increased range of the homologous chromosomal recombination frequency may be one of the possible factors for the thickening of seminiferous tubule basement membrane and even lumen occlusion in NOA patients.
authors.affiliation: Department of Urology, Peking University Third Hospital, Beijing 100191, China.
authors.forename: Lian-Ming
authors.initials: LM
authors.lastname: Zhao
authors.forename: Hui
authors.initials: H
authors.lastname: Jiang
authors.forename: Kai
authors.initials: K
authors.lastname: Hong
authors.forename: Min
authors.initials: M
authors.lastname: Lu
authors.forename: Wen-Hao
authors.initials: WH
authors.lastname: Tang
authors.forename: Jia-Ming
authors.initials: JM
authors.lastname: Mao
authors.forename: Qing-Ling
authors.initials: QL
authors.lastname: Yang
authors.forename: Lu-Lin
authors.initials: LL
authors.lastname: Ma
mesh_terms: D000328:Adult; D053713:Azoospermia / Q000235:genetics / Q000473:pathology*; D002869:Chromosome Aberrations; D006801:Humans; D008297:Male; D008540:Meiosis; D011995:Recombination, Genetic; D013090:Spermatocytes / Q000166:cytology*; D055815:Young Adult