title: Structural modelling studies and immunoprophylactic potential of Brugia malayi DEAD Box RNA helicase.
abstract: DEAD Box RNA helicases are essential enzymes that are involved in RNA metabolic processes such as transcription, pre-mRNA splicing, translation initiation and RNA decay. We have previously over-expressed and biochemically characterized an immunodominant cDNA clone encoding DEAD box RNA helicase (BmL3-Helicase) isolated by immunoscreening of the larval stage cDNA library of Brugia malayi. In the current study, the 3D structure was determined and the immunoprophylactic efficacy of BmL3-Helicase was investigated by immunizing Mastomys coucha with the recombinant protein and subsequently challenging with B. malayi infective larvae. The immunization had an adverse outcome on the establishment of challenged larvae resulting in a 67.4% reduction in adult parasite recovery, a 86.7% decrease in the microfilarial density and profound sterility of the recovered female worms. The immune response thus generated was investigated by measuring the levels of specific antibodies including IgG subclasses, reactive oxygen species and cytokines.
authors.affiliation: Division of Parasitology, CSIR-Central Drug Research Institute, BS10/1, Sector 10, Jankipuram extension, Sitapur Road, Lucknow 226021, Uttar Pradesh, India.
authors.forename: Meghna
authors.initials: M
authors.lastname: Singh
authors.forename: Nidhi
authors.initials: N
authors.lastname: Shrivastava
authors.forename: Uzma
authors.initials: U
authors.lastname: Saqib
authors.forename: Mohammad Imran
authors.initials: MI
authors.lastname: Siddiqi
authors.forename: Shailja
authors.initials: S
authors.lastname: Misra-Bhattacharya
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis / Q000097:blood*; D000947:Antigens, Helminth / Q000276:immunology*; D017178:Brugia malayi / Q000201:enzymology* / Q000235:genetics / Q000276:immunology; D016207:Cytokines / Q000032:analysis / Q000378:metabolism; D053487:DEAD-box RNA Helicases / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D004605:Elephantiasis, Filarial / Q000276:immunology* / Q000469:parasitology; D005260:Female; D015723:Gene Library; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D006801:Humans; D007114:Immunization; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D016130:Immunophenotyping; D007814:Larva; D008842:Microfilariae; D008958:Models, Molecular; D051189:Murinae; D009569:Nitric Oxide / Q000032:analysis / Q000378:metabolism; D017434:Protein Structure, Tertiary; D017382:Reactive Oxygen Species / Q000032:analysis / Q000378:metabolism; D011994:Recombinant Proteins
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D016207:Cytokines; D015801:Helminth Proteins; D007074:Immunoglobulin G; D017382:Reactive Oxygen Species; D011994:Recombinant Proteins; D009569:Nitric Oxide; D053487:DEAD-box RNA Helicases