title: [Construction and biological function of eukaryotic expression vector for myc-tagged HER2].
abstract: OBJECTIVE
  To construct eukaryotic expression vector of myc-tagged human HER2 and investigate its role in breast cancer cell proliferation and drug resistance.
  
  
  METHODS
  Human HER2 gene was amplified by PCR and cloned into the pXJ-40-myc vector. The recombinant myc-HER2 was identified by enzyme digestion and sequencing, transfected into breast cancer cell line ZR75-1, and then was detected by Western blotting. Next, CCK8 assay was performed to investigate myc-Her2 transfected cell proliferation. Trastuzumab, anti-Her2 antibody, was added to determine the sensitivity of cells transfected with myc-Her2.
  
  
  RESULTS
  Enzyme digestion and sequencing confirmed the myc-HER2 vector was constructed successfully. Western blotting showed the expression of myc-HER2 in the ZR75-1 cells. The result of cell growth curve showed that cells transfected with myc-her2 grew significantly faster than the control cells. Moreover, trastuzumab obviously suppressed the growth of ZR75-1 cells transfected with myc-HER2.
  
  
  CONCLUSIONS
  The myc-HER2 vector was successfully constructed which lays an experimental foundation for the study of HER2 drug resistance to trastuzumab.
authors.affiliation: Medical School of Chinese PLA, Beijing 100048, China. songjinjie520@sina.com
authors.forename: Jinjie
authors.initials: J
authors.lastname: Song
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Xiaojie
authors.initials: X
authors.lastname: Xu
authors.forename: Jing
authors.initials: J
authors.lastname: Fu
authors.forename: Jiahong
authors.initials: J
authors.lastname: Liu
authors.forename: Qinong
authors.initials: Q
authors.lastname: Ye
authors.forename: Zefei
authors.initials: Z
authors.lastname: Jiang
mesh_terms: D061067:Antibodies, Monoclonal, Humanized / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D015870:Gene Expression; D018734:Genes, erbB-2; D016259:Genes, myc; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D000068878:Trastuzumab
chemical_list: D061067:Antibodies, Monoclonal, Humanized; D011993:Recombinant Fusion Proteins; D000068878:Trastuzumab