title: Inhibition of Quorum Sensing Mediated Virulence Factors Production in Urinary Pathogen Serratia marcescens PS1 by Marine Sponges.
abstract: The focal intent of this study was to find out an alternative strategy for the antibiotic usage against bacterial infections. The quorum sensing inhibitory (QSI) activity of marine sponges collected from Palk Bay, India was evaluated against acyl homoserine lactone (AHL) mediated violacein production in Chromobacterium violaceum (ATCC 12472), CV026 and virulence gene expressions in clinical isolate Serratia marcescens PS1. Out of 29 marine sponges tested, the methanol extracts of Aphrocallistes bocagei (TS 8), Haliclona (Gellius) megastoma (TS 25) and Clathria atrasanguinea (TS 27) inhibited the AHL mediated violacein production in C. violaceum (ATCC 12472) and CV026. Further, these sponge extracts inhibited the AHL dependent prodigiosin pigment, virulence enzymes such as protease, hemolysin production and biofilm formation in S. marcescens PS1. However, these sponge extracts were not inhibitory to bacterial growth, which reveals the fact that the QSI activity of these extracts was not related to static or killing effects on bacteria. Based on the obtained results, it is envisaged that the marine sponges could pave the way to prevent quorum sensing (QS) mediated bacterial infections.
authors.affiliation: Department of Biotechnology, Alagappa University, Karaikudi, 630 003 Tamil Nadu India.
authors.forename: Angusamy
authors.initials: A
authors.lastname: Annapoorani
authors.forename: Abdul Karim Kamil Abdul
authors.initials: AK
authors.lastname: Jabbar
authors.forename: Syed Khadar Syed
authors.initials: SK
authors.lastname: Musthafa
authors.forename: Shunmugiah Karutha
authors.initials: SK
authors.lastname: Pandian
authors.forename: Arumugam Veera
authors.initials: AV
authors.lastname: Ravi
keywords: Acyl homoserine lactone; Bacterial pathogenesis; Biofilm; Marine sponges; Quorum sensing inhibition; Serratia marcescens PS1