title: Differentiation of hUC-MSC into dopaminergic-like cells after transduction with hepatocyte growth factor.
abstract: Parkinson's disease (PD) is a common neurodegenerative condition causing significant disability and thus negatively impacting quality of life. The recent advent of stem cell-based therapy has heralded the prospect of a potential restorative treatment option for PD. In particular, mesenchymal stem cells derived from human umbilical cord (hUC-MSCs) have great potential for developing a therapeutic agent as such. Furthermore, hepatocyte growth factor (HGF), which shows mitogenic and morphogenetic activities in a variety of cells, including MSC, and may be implicated in the pathophysiology of PD. As such, HGF may represent a new therapeutic target for the disease. In this study, we successfully isolated and facilitated the transduction of an adenoviral vector expressing HGF (Ad-HGF) into isolated hUC-MSCs. Following transduction, the hUC-MSCs can differentiate into dopaminergic neuron-like cells secreting dopamine, tyrosine hydroxylase, and dopamine transporter. Our data suggest that hUC-MSCs have the ability to differentiate into dopaminergic neurons after transduction with Ad-HGF, providing encouraging evidence to further explore this approach to the treatment of PD.
authors.affiliation: The Neurology Department of the 148th Hospital of PLA, Zibo, People's Republic of China. lijinfeng1978@yahoo.com.cn
authors.forename: Jin-Feng
authors.initials: JF
authors.lastname: Li
authors.forename: Hong-Lei
authors.initials: HL
authors.lastname: Yin
authors.forename: Adam
authors.initials: A
authors.lastname: Shuboy
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Duan
authors.forename: Ji-Yu
authors.initials: JY
authors.lastname: Lou
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Hong-wei
authors.initials: HW
authors.lastname: Wang
authors.forename: Yun-Liang
authors.initials: YL
authors.lastname: Wang
mesh_terms: D000256:Adenoviridae; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation; D002469:Cell Separation; D004298:Dopamine / Q000378:metabolism; D050483:Dopamine Plasma Membrane Transport Proteins / Q000378:metabolism; D059290:Dopaminergic Neurons / Q000166:cytology* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D005822:Genetic Vectors; D017228:Hepatocyte Growth Factor / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008565:Membrane Proteins / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D014161:Transduction, Genetic; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism; D014470:Umbilical Cord / Q000166:cytology*
chemical_list: D015415:Biomarkers; D050483:Dopamine Plasma Membrane Transport Proteins; D008565:Membrane Proteins; D017228:Hepatocyte Growth Factor; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine