title: Highly enantioselective oxidation of racemic phenyl-1,2-ethanediol to optically pure (R)-(-)-mandelic acid by a newly isolated Brevibacterium lutescens CCZU12-1.
abstract: Enantioselective oxidation of racemic phenyl-1,2-ethanediol into (R)-(-)-mandelic acid by a newly isolated Brevibacterium lutescens CCZU12-1 was demonstrated. It was found that optically active (R)-(-)-mandelic acid (e.e.p > 99.9 %) is produced leaving the other enantiomer (S)-(+)-phenyl-1,2-ethanediol intact. Using fed-batch method, a total of 172.9 mM (R)-(-)-mandelic acid accumulated in the reaction mixture after the seventh feed. Moreover, oxidation of phenyl-1,2-ethanediol using calcium alginate-entrapped resting cells was carried out in the aqueous system, and efficient biocatalyst recycling was achieved as a result of cell immobilization in calcium alginate, with a product-to-biocatalyst ratio of 27.94 g (R)-(-)-mandelic acid g⁻¹ dry cell weight cell after 16 cycles of repeated use.
authors.affiliation: Laboratory of Biochemical Engineering, College of Pharmaceutical and Life Sciences, Changzhou University, Changzhou 213164, China. heyucai2001@163.com
authors.forename: Yu-Cai
authors.initials: YC
authors.lastname: He
authors.forename: Cui-Luan
authors.initials: CL
authors.lastname: Ma
authors.forename: Xian
authors.initials: X
authors.lastname: Zhang
authors.forename: Liang
authors.initials: L
authors.lastname: Li
authors.forename: Jian-He
authors.initials: JH
authors.lastname: Xu
authors.forename: Miao-Xin
authors.initials: MX
authors.lastname: Wu
mesh_terms: D000464:Alginates; D001951:Brevibacterium / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D018914:Cells, Immobilized / Q000378:metabolism; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005026:Ethylene Glycols / Q000378:metabolism*; D020723:Glucuronic Acid; D006603:Hexuronic Acids; D008333:Mandelic Acids / Q000378:metabolism*; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013237:Stereoisomerism; D013379:Substrate Specificity
chemical_list: D000464:Alginates; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005026:Ethylene Glycols; D006603:Hexuronic Acids; D008333:Mandelic Acids; D012336:RNA, Ribosomal, 16S; C031474:styrene glycol; D020723:Glucuronic Acid; C037938:mandelic acid