title: Comparison of anticancer activity between lactoferrin nanoliposome and lactoferrin in Caco-2 cells in vitro.
abstract: The anticancer activities of Lactoferrin (Lf) and Lf nanoliposomes in Caco-2 cells were observed in this study, and mitochondrial function (MTT assay), count kit-8(CCK-8), detection of intracellular reactive oxygen species (ROS) and apoptosis induction (AO/EB staining) assays were used to evaluate the anticancer activity. MTT results demonstrated that Lf nanoliposomes and Lf reduced the mitochondrial activity of cells in a manner of dose and time effect, and the viabilities of Caco-2 cell were significantly decreased in vitro following exposure to Lf nanoliposomes at the concentrations of 5 and 10 mg/mL. LDH leakage and ROS significantly increased in cells exposed to Lf nanoliposomes (≥5 mg/mL), while Lf induced ROS only at higher doses (10mg/mL). CCK-8 evaluation of cell proliferation and AO/EB double staining supported the anti-proliferative effects of Lf liposomes. Our findings demonstrated that the presence of Lf nanoliposome is more significant than Lf in inhibiting human tumor cells proliferation. Therefore, it can be concluded that Lf nanoliposomes are a potential therapeutic modality in the management of tumors.
authors.affiliation: Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, China Jiliang University, Hangzhou 310018, PR China.
authors.forename: Jieqing
authors.initials: J
authors.lastname: Ma
authors.forename: Rongfa
authors.initials: R
authors.lastname: Guan
authors.forename: Haitao
authors.initials: H
authors.lastname: Shen
authors.forename: Fei
authors.initials: F
authors.lastname: Lu
authors.forename: Chaogeng
authors.initials: C
authors.lastname: Xiao
authors.forename: Mingqi
authors.initials: M
authors.lastname: Liu
authors.forename: Tianshu
authors.initials: T
authors.lastname: Kang
mesh_terms: D000970:Antineoplastic Agents / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D018938:Caco-2 Cells; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D004355:Drug Stability; D004795:Enzyme Stability; D006801:Humans; D007700:Kinetics; D007781:Lactoferrin / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology* / Q000648:ultrastructure; D015227:Lipid Peroxidation / Q000187:drug effects; D008081:Liposomes; D046529:Microscopy, Electron, Transmission; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D054118:Nanospheres; D009363:Neoplasm Proteins / Q000378:metabolism; D010316:Particle Size; D017382:Reactive Oxygen Species / Q000819:agonists / Q000378:metabolism; D011949:Receptors, Cholecystokinin / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D008081:Liposomes; D009363:Neoplasm Proteins; D017382:Reactive Oxygen Species; D011949:Receptors, Cholecystokinin; D007781:Lactoferrin
keywords: Caco-2; Cell viability; Lactoferrin nanoliposomes; MTT