title: [Establishment of the detection method for two causative genes of cattle arachnomelia syndrome].
abstract: Arachnomelia syndrome (AS) is a recessive inherited disease in cattle. Although the arachnomelia phenotypes are virtually identical in Brown Swiss and Simmental cattle, the causative mutation are different, which are a 1 bp insertion c.363-364insG in the sulfite oxidase (SUOX) gene and a 2 bp deletion c.1224_1225delCA in the molybdenum cofactor syn-thesis step 1 (MOCS1) gene, respectively. In the current study, combining fluorescence PCR with capillary electrophoresis technology, an automatic fluorescence method was established, which could detect the two causative loci rapidly and cor-rectly with a single reaction. Samples from 51 Simmental bulls, 80 cows mated artificially using semen of Simmental bulls and their resulted 106 progeny, together with 55 Xinjiang Brown were collected and used for validation of the newly de-signed methods. Our results have laid a foundation for screening AS disease causing mutations in Chinese cattle.
authors.affiliation: Beijing Academy of Agriculture and Forestry Sciences, Beijing, China. chuqinsd@163.com
authors.forename: Qin
authors.initials: Q
authors.lastname: Chu
authors.forename: Shi-Hui
authors.initials: SH
authors.lastname: Jiao
authors.forename: Ya-Chun
authors.initials: YC
authors.lastname: Wang
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.forename: Ai-Rong
authors.initials: AR
authors.lastname: Liu
authors.forename: Hong-Jun
authors.initials: HJ
authors.lastname: Wu
authors.forename: Zhen-Quan
authors.initials: ZQ
authors.lastname: Xie
authors.forename: Shi-Yu
authors.initials: SY
authors.lastname: Hou
authors.forename: Fan-Jun
authors.initials: FJ
authors.lastname: Geng
authors.forename: Cong-Yong
authors.initials: CY
authors.lastname: Wang
authors.forename: Xi-Xia
authors.initials: XX
authors.lastname: Huang
authors.forename: Shi-Xin
authors.initials: SX
authors.lastname: Tan
authors.forename: Rui
authors.initials: R
authors.lastname: Tan
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Ying
authors.initials: Y
authors.lastname: Yu
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000151:congenital / Q000175:diagnosis / Q000235:genetics*; D003067:Coenzymes / Q000235:genetics*; D005260:Female; D017880:Limb Deformities, Congenital / Q000175:diagnosis / Q000235:genetics / Q000662:veterinary*; D008297:Male; D008667:Metalloproteins / Q000235:genetics*; D000090006:Molybdenum Cofactors; D016254:Mutagenesis, Insertional; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011621:Pteridines; D017384:Sequence Deletion
chemical_list: D003067:Coenzymes; D008667:Metalloproteins; D000090006:Molybdenum Cofactors; D050862:Oxidoreductases Acting on Sulfur Group Donors; D011621:Pteridines; C022261:molybdopterin; C080499:sulfide oxidase