title: Tb(3+)-tRNA for LRET studies of protein synthesis.
abstract: When suitably labeled bulk tRNAs are transfected into cells they give rise to FRET (fluorescence resonance energy transfer) signals via binding to ribosomes that provide a measure of total protein synthesis. Application of this approach to monitoring rates of specific protein synthesis requires achieving a very high signal-to-noise ratio. Such high ratios may be attainable using LRET (luminescence resonance energy transfer) in place of FRET. Lanthanide complexes containing an antenna chromophore are excellent LRET donors. Here we describe the synthesis of a Phe-tRNA(Phe) labeled with a Tb(3+) complex, denoted Tb(3+)-Phe-tRNA(Phe) that, notwithstanding the bulkiness of the Tb(3+) complex, is active in protein synthesis.
authors.affiliation: Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104-6323, USA.
authors.forename: Dulce
authors.initials: D
authors.lastname: Alonso
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Gabriel
authors.initials: G
authors.lastname: Rosenblum
authors.forename: Tomoyasu
authors.initials: T
authors.lastname: Mani
authors.forename: Yale E
authors.initials: YE
authors.lastname: Goldman
authors.forename: Barry S
authors.initials: BS
authors.lastname: Cooperman
mesh_terms: D004735:Energy Transfer; D009682:Magnetic Resonance Spectroscopy; D014176:Protein Biosynthesis; D012343:RNA, Transfer / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013725:Terbium / Q000737:chemistry*
chemical_list: D013725:Terbium; D012343:RNA, Transfer