title: Methods for the study of entosis.
abstract: Entosis is a recently described nonapoptotic cell death mechanism that is initiated by the engulfment of live epithelial cells, leading to the formation of "cell-in-cell" structures. Entotic cell engulfment is induced by matrix detachment, and is driven by imbalances in actomyosin contraction between neighboring cells. Here we describe methods to quantify the formation of cell-in-cell structures by entosis, for cells cultured in suspension or in soft agar, by fluorescence imaging and time-lapse microscopy.
authors.affiliation: Cell Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY, USA.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Sun
authors.forename: Michael
authors.initials: M
authors.lastname: Overholtzer
mesh_terms: D002460:Cell Line; D003584:Cytological Techniques / Q000379:methods*; D057691:Entosis / Q000502:physiology*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D059008:Time-Lapse Imaging