title: Oxygen promotes biofilm formation of Shewanella putrefaciens CN32 through a diguanylate cyclase and an adhesin.
abstract: Although oxygen has been reported to regulate biofilm formation by several Shewanella species, the exact regulatory mechanism mostly remains unclear. Here, we identify a direct oxygen-sensing diguanylate cyclase (DosD) and reveal its regulatory role in biofilm formation by Shewanella putrefaciens CN32 under aerobic conditions. In vitro and in vivo analyses revealed that the activity of DosD culminates to synthesis of cyclic diguanylate (c-di-GMP) in the presence of oxygen. DosD regulates the transcription of bpfA operon which encodes seven proteins including a large repetitive adhesin BpfA and its cognate type I secretion system (TISS). Regulation of DosD in aerobic biofilms is heavily dependent on an adhesin BpfA and the TISS. This study offers an insight into the molecular mechanism of oxygen-stimulated biofilm formation by S. putrefaciens CN32.
authors.affiliation: Department of Chemistry, University of Science & Technology of China, Hefei, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wu
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Cheng
authors.forename: Hao
authors.initials: H
authors.lastname: Yin
authors.forename: Xiang-Ning
authors.initials: XN
authors.lastname: Song
authors.forename: Wen-Wei
authors.initials: WW
authors.lastname: Li
authors.forename: Xian-Xuan
authors.initials: XX
authors.lastname: Zhou
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Zhao
authors.forename: Li-Jiao
authors.initials: LJ
authors.lastname: Tian
authors.forename: Jun-Cheng
authors.initials: JC
authors.lastname: Han
authors.forename: Han-Qing
authors.initials: HQ
authors.lastname: Yu
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D018441:Biofilms / Q000254:growth & development*; D015374:Biosensing Techniques; D015153:Blotting, Western; D006152:Cyclic GMP / Q000031:analogs & derivatives / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D009876:Operon / Q000235:genetics; D010100:Oxygen / Q000494:pharmacology*; D019761:Phosphorus-Oxygen Lyases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020614:Shewanella putrefaciens / Q000235:genetics / Q000378:metabolism*
chemical_list: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; D012333:RNA, Messenger; C062025:bis(3',5')-cyclic diguanylic acid; D019761:Phosphorus-Oxygen Lyases; C060720:diguanylate cyclase; D006152:Cyclic GMP; D010100:Oxygen