title: The MOV10 helicase inhibits LINE-1 mobility.
abstract: LINE-1 (long interspersed element 1) is an autonomous non-long terminal repeat retrotransposon. Its replication often causes mutation and rearrangement of host genomic DNA. Accordingly, host cells have evolved mechanisms to control LINE-1 mobility. Here, we report that a helicase named MOV10 effectively suppresses LINE-1 transposition. Mutating the helicase motifs impairs this function of MOV10, suggesting that MOV10 requires its helicase activity to suppress LINE-1 replication. Further studies show that MOV10 post-transcriptionally diminishes the level of LINE-1 RNA. The association of MOV10 with both LINE-1 RNA and ORF1 suggests that MOV10 interacts with LINE-1 RNP and consequently causes its RNA degradation. These data demonstrate collectively that MOV10 contributes to the cellular control of LINE-1 replication.
authors.affiliation: From the Institute of Medicinal Biotechnology and.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Li
authors.affiliation: the Lady Davis Institute-Jewish General Hospital, Montreal, Quebec H3T 1E2, Canada,; the Departments of Medicine and.
authors.forename: Jianyong
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Nankai University, Tianjin 300071, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Jia
authors.affiliation: the Lady Davis Institute-Jewish General Hospital, Montreal, Quebec H3T 1E2, Canada,; Microbiology and Immunology, McGill University, Montreal, Quebec H3A 2B4, Canada, and.
authors.forename: Vicky
authors.initials: V
authors.lastname: Cheng
authors.affiliation: Nankai University, Tianjin 300071, China; the Institute of Pathogen Biology, Chinese Academy of Medical Science, Beijing 100050, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Xu
authors.affiliation: Nankai University, Tianjin 300071, China.
authors.forename: Wentao
authors.initials: W
authors.lastname: Qiao
authors.affiliation: the Institute of Pathogen Biology, Chinese Academy of Medical Science, Beijing 100050, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Guo
authors.affiliation: From the Institute of Medicinal Biotechnology and; the Departments of Medicine and; Microbiology and Immunology, McGill University, Montreal, Quebec H3A 2B4, Canada, and. Electronic address: chen.liang@mcgill.ca.
authors.forename: Chen
authors.initials: C
authors.lastname: Liang
authors.affiliation: From the Institute of Medicinal Biotechnology and. Electronic address: shancen@imb.pumc.edu.cn.
authors.forename: Shan
authors.initials: S
authors.lastname: Cen
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics; D000818:Animals; D060565:Argonaute Proteins / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D055785:Gene Knockdown Techniques; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D020084:Long Interspersed Nucleotide Elements / Q000235:genetics*; D051379:Mice; D011401:Promoter Regions, Genetic / Q000235:genetics; D012313:RNA / Q000096:biosynthesis; D020365:RNA Helicases / Q000378:metabolism*; D020871:RNA Stability / Q000235:genetics
chemical_list: D020121:5' Untranslated Regions; C466422:AGO2 protein, human; D060565:Argonaute Proteins; D018076:DNA, Complementary; D012313:RNA; C530476:Mov10 protein, human; D020365:RNA Helicases
keywords: Host-Pathogen Interactions; LINE-1; MOV10; Post-transcriptional; RNA Helicase; Restriction; Retrotransposon; Reverse Transcription; Viral Replication; Virology