title: Preparation of a novel carboxyl stationary phase by "thiol-ene" click chemistry for hydrophilic interaction chromatography.
abstract: A novel carboxyl-bonded silica stationary phase was prepared by "thiol-ene" click chemistry. The resultant Thiol-Click-COOH phase was evaluated under hydrophilic interaction liquid chromatography (HILIC) mobile phase conditions. A comparison of the chromatographic performance of Thiol-Click-COOH and pure silica columns was performed according to the retention behaviors of analytes and the charged state of the stationary phases. The results indicated that the newly developed Thiol-Click-COOH column has a higher surface charge and stronger hydrophilicity than the pure silica column. Furthermore, the chromatographic behaviors of five nucleosides on the Thiol-Click-COOH phase were investigated in detail. Finally, a good separation of 13 nucleosides and bases, and four water-soluble vitamins was achieved.
authors.affiliation: Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan, China.
authors.forename: Xi-Tian
authors.initials: XT
authors.lastname: Peng
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.forename: Shu-Xian
authors.initials: SX
authors.lastname: Ji
authors.forename: Yu-Qi
authors.initials: YQ
authors.lastname: Feng
mesh_terms: D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods; D057930:Click Chemistry; D057927:Hydrophobic and Hydrophilic Interactions; D009705:Nucleosides / Q000302:isolation & purification*; D012117:Resins, Synthetic / Q000138:chemical synthesis / Q000737:chemistry*; D014815:Vitamins / Q000302:isolation & purification*
chemical_list: D009705:Nucleosides; D012117:Resins, Synthetic; D014815:Vitamins
keywords: Click chemistry; Hydrophilic interaction chromatography; Nucleosides; Retention mechanisms; Water-soluble vitamins