title: Stepwise evolution of essential centromere function in a Drosophila neogene.
abstract: Evolutionarily young genes that serve essential functions represent a paradox; they must perform a function that either was not required until after their birth or was redundant with another gene. How young genes rapidly acquire essential function is largely unknown. We traced the evolutionary steps by which the Drosophila gene Umbrea acquired an essential role in chromosome segregation in D. melanogaster since the gene's origin less than 15 million years ago. Umbrea neofunctionalization occurred via loss of an ancestral heterochromatin-localizing domain, followed by alterations that rewired its protein interaction network and led to species-specific centromere localization. Our evolutionary cell biology approach provides temporal and mechanistic detail about how young genes gain essential function. Such innovations may constantly alter the repertoire of centromeric proteins in eukaryotes.
authors.affiliation: Molecular and Cellular Biology Program, University of Washington, Seattle, WA 98195, USA.
authors.forename: Benjamin D
authors.initials: BD
authors.lastname: Ross
authors.forename: Leah
authors.initials: L
authors.lastname: Rosin
authors.forename: Andreas W
authors.initials: AW
authors.lastname: Thomae
authors.forename: Mary Alice
authors.initials: MA
authors.lastname: Hiatt
authors.forename: Danielle
authors.initials: D
authors.lastname: Vermaak
authors.forename: Aida Flor A
authors.initials: AF
authors.lastname: de la Cruz
authors.forename: Axel
authors.initials: A
authors.lastname: Imhof
authors.forename: Barbara G
authors.initials: BG
authors.lastname: Mellone
authors.forename: Harmit S
authors.initials: HS
authors.lastname: Malik
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002503:Centromere / Q000235:genetics / Q000502:physiology*; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics*; D004330:Drosophila / Q000235:genetics*; D029721:Drosophila Proteins / Q000235:genetics*; D019143:Evolution, Molecular; D020440:Gene Duplication; D017344:Genes, Insect / Q000502:physiology*; D008969:Molecular Sequence Data
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D029721:Drosophila Proteins; C539839:HP6 protein, Drosophila