title: MiR-26a inhibits proliferation and migration of breast cancer through repression of MCL-1.
abstract: Breast cancer is the most commonly malignancies in women. MicroRNAs are a family of small non-coding RNAs 18-25 nucleotides in length that post-transcriptionally modulate gene expression. MiR-26a has been reported as a tumor suppressor microRNA in breast cancer, which is attributed mainly to targeting of MTDH and EZH2, however, the expression profile and therapeutic potential of miR-26a is still unclear. Here we demonstrate that miR-26a is down-regulated in breast cancer cells and clinical specimens and its modulation in breast cancer cells regulates cell proliferation, colony formation, migration and apoptosis. MCL-1, an anti-apoptotic member of the Bcl-2 family, as novel targets of miR-26a was found to be in reverse correlation with ectopic expression of miR-26a and knockdown of MCL-1 phenocopied the effect of miR-26a in breast cancer cell lines. It was further explored that miR-26a increased sensitivity of breast cancer cells to paclitaxel in which MCL-1 was involved. Thus, miR-26a impacts on cell proliferation and migration of breast cancer by regulating several carcinogenesis-related processes, including a novel mechanism involving the targeting of MCL-1.
authors.affiliation: Department of Breast Oncology, Sun Yat-sen University Cancer Center, Guangzhou, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
authors.forename: Laisheng
authors.initials: L
authors.lastname: Li
authors.forename: Minqing
authors.initials: M
authors.lastname: Wu
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.forename: Xinhua
authors.initials: X
authors.lastname: Xie
authors.forename: Jiaoli
authors.initials: J
authors.lastname: Guo
authors.forename: Hailin
authors.initials: H
authors.lastname: Tang
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Xie
mesh_terms: D000328:Adult; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D001483:Base Sequence; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects / Q000235:genetics*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics*; D005260:Female; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D064549:Myeloid Cell Leukemia Sequence 1 Protein / Q000235:genetics*; D017239:Paclitaxel / Q000494:pharmacology
chemical_list: D035683:MicroRNAs; D064549:Myeloid Cell Leukemia Sequence 1 Protein; D017239:Paclitaxel; C579095:MCL1 protein, human; C528163:MIRN26 microRNA, human