title: Novel neutralising antibodies targeting the N-terminal helical region of the transmembrane envelope protein p15E of the porcine endogenous retrovirus (PERV).
abstract: Previously, immunising different species with the transmembrane envelope protein p15E of the porcine endogenous retrovirus (PERV), neutralising antibodies were induced which recognised epitopes in the fusion peptide proximal region (FPPR) and in the membrane-proximal external region (MPER). Only the MPER-specific antibodies were shown to neutralise and these antibodies targeted epitopes in the MPER similarly localised as the epitopes recognised by antibodies broadly neutralising HIV-1 such as 2F5 and 4E10. To study whether neutralising antibodies could be induced immunising with subunits of p15E, recombinant proteins corresponding to the N-terminal, the C-terminal helical region (NHR, CHR) and a p15E with a mutation in the Cys-Cys loop were produced. Whereas none of these antigens induced MPER-specific neutralising antibodies, the animals immunised with the FPPR/NHR subunit and the mutated p15E produced neutralising antibodies binding to the NHR. Therefore, for the first time, antibodies specific for the NHR and neutralising PERV were described.
authors.affiliation: Centre for HIV and Other Retroviruses, Robert Koch Institute, Nordufer 20, 13353, Berlin, Germany.
authors.forename: Alexander
authors.initials: A
authors.lastname: Waechter
authors.forename: Joachim
authors.initials: J
authors.lastname: Denner
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood / Q000276:immunology; D020077:Endogenous Retroviruses / Q000235:genetics / Q000276:immunology*; D018604:Epitope Mapping; D006041:Goats; D007114:Immunization / Q000379:methods* / Q000592:standards; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009500:Neutralization Tests; D011817:Rabbits; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology*; D012192:Retroviridae Infections / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000821:virology*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology* / Q000494:pharmacology
chemical_list: D057134:Antibodies, Neutralizing; D011994:Recombinant Proteins; D014759:Viral Envelope Proteins; D014765:Viral Vaccines