title: Performance of scientific cameras with different sensor types in measuring dynamic processes in fluorescence microscopy.
abstract: The plethora of available scientific cameras of different types challenges the biologically oriented experimenter when picking the appropriate camera for his experiment. In this study, we chose to investigate camera performances in a typical nonsingle molecule situation in life sciences, that is, quantitative measurements of fluorescence intensity changes from video data with typically skewed intensity distributions. Here, intensity profile dynamics of pH-sensors upon triggered changes of pH-environments in living cells served as a model system. The following camera types were tested: sCMOS, CCD (scientific and nonscientific) and EM-CCD (back- and front-illuminated). We found that although the EM-CCD cameras achieved the best absolute spatial SNR (signal-to-noise ratio) values, the sCMOS was at least of equal performance when the spatial SNR was related to the effective dynamic range, and it was superior in terms of temporal SNR. In the measurements of triggered intensity changes, the sCMOS camera had the advantage that it used the smallest fraction of its dynamic range when depicting intensity changes, and thus featured the best SNR at full usage of its dynamic range.
authors.affiliation: Department of Psychiatry and Psychotherapy, Friedrich-Alexander-University of Erlangen-Nuremberg, Erlangen, Germany.
authors.forename: Jasmin
authors.initials: J
authors.lastname: Jung
authors.forename: Siegfried
authors.initials: S
authors.lastname: Weisenburger
authors.forename: Sahradha
authors.initials: S
authors.lastname: Albert
authors.forename: Daniel F
authors.initials: DF
authors.lastname: Gilbert
authors.forename: Oliver
authors.initials: O
authors.lastname: Friedrich
authors.forename: Volker
authors.initials: V
authors.lastname: Eulenburg
authors.forename: Johannes
authors.initials: J
authors.lastname: Kornhuber
authors.forename: Teja W
authors.initials: TW
authors.lastname: Groemer
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D049452:Green Fluorescent Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D009474:Neurons / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D049452:Green Fluorescent Proteins
keywords: CCD; CMOS; fluorescence microscopy; time-resolved imaging