title: Clinical significance of vimentin expression and Her-2 status in patients with gastric carcinoma.
abstract: OBJECTIVE
  To determine whether vimentin could be used as a marker of gastric carcinomas with more aggressive behavior. To detect the extent of Her-2 status in gastric carcinoma and explore the correlation between vimentin expression and Her-2 status.
  
  
  METHODS
  Vimentin expression was detected in surgically resected gastric carcinoma tissue specimens from 143 patients by immunohistochemistry. The human epidermal growth factor receptor 2 (Her2) status was evaluated by fluorescence in situ hybridization (FISH). Correlations between vimentin expression, Her-2 status and clinicopathological factors were evaluated using Kaplan-Meier method and Cox multivariate survival models.
  
  
  RESULTS
  Vimentin expression was significantly correlated with age, advanced stage, poorly differentiated type, venous invasion, hepatic metastasis, and recurrence (p < 0.05). Her-2 gene was amplified in 16 (11.2%) out of the 143 gastric carcinoma tissue specimens. Her-2 status was correlated with advanced cancer, poor differentiation, venous invasion, hepatic metastasis, and recurrence (p < 0.05). The result of multivariate analysis showed that vimentin expression and lymph node metastasis were independent prognostic factors.
  
  
  CONCLUSIONS
  Vimentin expression in epithelial cells of the surgically resected gastric adenocarcinoma tissue is an independent predictor of short survival, and Her-2 status shows a valuable correlation with clinical parameters.
authors.affiliation: Department of Oncology, Affiliated Hospital of Medical College, Qingdao University, Qingdao, 266003, China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Zhao
authors.forename: Lu
authors.initials: L
authors.lastname: Yue
authors.forename: Fang
authors.initials: F
authors.lastname: Zhou
authors.forename: Congcong
authors.initials: C
authors.lastname: Xu
authors.forename: Wanhua
authors.initials: W
authors.lastname: Liang
authors.forename: Aihua
authors.initials: A
authors.lastname: Sui
authors.forename: Aiping
authors.initials: A
authors.lastname: Ding
authors.forename: Wensheng
authors.initials: W
authors.lastname: Qiu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003593:Cytoplasm / Q000378:metabolism / Q000473:pathology; D005260:Female; D005784:Gene Amplification; D006801:Humans; D008207:Lymphatic Metastasis / Q000473:pathology; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D016019:Survival Analysis; D014746:Vimentin / Q000378:metabolism*
chemical_list: D018719:Erb-b2 Receptor Tyrosine Kinases; D014746:Vimentin; C508053:ERBB2 protein, human