title: Short-term caloric restriction, resveratrol, or combined treatment regimens initiated in late-life alter mitochondrial protein expression profiles in a fiber-type specific manner in aged animals.
abstract: Aging is associated with a loss in muscle known as sarcopenia that is partially attributed to apoptosis. In aging rodents, caloric restriction (CR) increases health and longevity by improving mitochondrial function and the polyphenol resveratrol (RSV) has been reported to have similar benefits. In the present study, we investigated the potential efficacy of using short-term (6 weeks) CR (20%), RSV (50 mg/kg/day), or combined CR+ RSV (20% CR and 50 mg/kg/day RSV), initiated at late-life (27 months) to protect muscle against sarcopenia by altering mitochondrial function, biogenesis, content, and apoptotic signaling in both glycolytic white and oxidative red gastrocnemius muscle (WG and RG, respectively) of male Fischer 344 × Brown Norway rats. CR but not RSV attenuated the age-associated loss of muscle mass in both mixed gastrocnemius and soleus muscle, while combined treatment (CR + RSV) paradigms showed a protective effect in the soleus and plantaris muscle (P < 0.05). Sirt1 protein content was increased by 2.6-fold (P < 0.05) in WG but not RG muscle with RSV treatment, while CR or CR + RSV had no effect. PGC-1α levels were higher (2-fold) in the WG from CR-treated animals (P < 0.05) when compared to ad-libitum (AL) animals but no differences were observed in the RG with any treatment. Levels of the anti-apoptotic protein Bcl-2 were significantly higher (1.6-fold) in the WG muscle of RSV and CR + RSV groups compared to AL (P < 0.05) but tended to occur coincident with elevations in the pro-apoptotic protein Bax so that the apoptotic susceptibility as indicated by the Bax to Bcl-2 ratio was unchanged. There were no alterations in DNA fragmentation with any treatment in muscle from older animals. Additionally, mitochondrial respiration measured in permeabilized muscle fibers was unchanged in any treatment group and this paralleled the lack of change in cytochrome c oxidase (COX) activity. These data suggest that short-term moderate CR, RSV, or CR + RSV tended to modestly alter key mitochondrial regulatory and apoptotic signaling pathways in glycolytic muscle and this might contribute to the moderate protective effects against aging-induced muscle loss observed in this study.
authors.affiliation: Department of Aging and Geriatric Research, Division of Biology of Aging, University of Florida, Gainesville, FL 32611, USA. amjoseph@ufl.edu
authors.forename: Anna-Maria
authors.initials: AM
authors.lastname: Joseph
authors.forename: Angelina G
authors.initials: AG
authors.lastname: Malamo
authors.forename: Jason
authors.initials: J
authors.lastname: Silvestre
authors.forename: Nick
authors.initials: N
authors.lastname: Wawrzyniak
authors.forename: Sean
authors.initials: S
authors.lastname: Carey-Love
authors.forename: Linda M-D
authors.initials: LM
authors.lastname: Nguyen
authors.forename: Debapriya
authors.initials: D
authors.lastname: Dutta
authors.forename: Jinze
authors.initials: J
authors.lastname: Xu
authors.forename: Christiaan
authors.initials: C
authors.lastname: Leeuwenburgh
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Adhihetty
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000378:metabolism; D000375:Aging / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D031204:Caloric Restriction; D003131:Combined Modality Therapy; D008297:Male; D008931:Mitochondria, Muscle / Q000187:drug effects / Q000201:enzymology / Q000502:physiology; D024101:Mitochondrial Proteins / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism / Q000473:pathology; D009929:Organ Size / Q000187:drug effects; D010101:Oxygen Consumption / Q000502:physiology; D051381:Rats; D011916:Rats, Inbred F344; D000077185:Resveratrol; D055948:Sarcopenia / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D056564:Sirtuin 1 / Q000378:metabolism; D013267:Stilbenes / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D051017:Apoptosis Regulatory Proteins; D024101:Mitochondrial Proteins; D013267:Stilbenes; D055372:AMP-Activated Protein Kinases; D056564:Sirtuin 1; D000077185:Resveratrol
keywords: Aging; Apoptosis; Biogenesis; Caloric restriction; Sarcopenia; Sirtuins