title: Coumarins in horse chestnut flowers: isolation and quantification by UPLC method.
abstract: The coumarins: scopoletin, esculetin and fraxetin were isolated from the flowers of horse chestnut (Aesculus hippocastanum L., Hippocastanaceae) and identified by spectrophotometric methods (UV, 1H, 13C NMR, ESI-MS). Their content, determined using the Ultra Performance Liquid Chromatography (UPLC), was 0.41, 0.13 and 0.05%, respectively.
authors.affiliation: Department of Pharmacognosy, University of Medical Sciences, Swiecickiego 4, 60-781 Poznań, Poland. dudum@poczta.onet.pl
authors.forename: Marlena
authors.initials: M
authors.lastname: Dudek-Makuch
authors.forename: Irena
authors.initials: I
authors.lastname: Matławska
mesh_terms: D031319:Aesculus / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D003374:Coumarins / Q000032:analysis / Q000302:isolation & purification*; D035264:Flowers / Q000737:chemistry
chemical_list: D003374:Coumarins