title: [Expression of FOS protein in glioma and its effect on the growth of human glioma cells].
abstract: OBJECTIVE
  To study the expression of FOS protein in human glioma tissues and its effect on tumor growth.
  
  
  METHODS
  FOS protein expression in glioma tissues was determined with immunohistochemical (IHC) staining. Subsequently, 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-diphenytetrazoliumromide (MTT) assay, flow cytometry, transwell invasion and Western blotting were used to assay U87 and U251 cells with reduced FOS expression.
  
  
  RESULTS
  The expression of FOS in glioma was increased and strongly correlated with its pathological grade. Abrogating expression of FOS has suppressed proliferation and invasion, and delayed cell cycle at G1 phrase for both U87 and U251 cells.
  
  
  CONCLUSIONS
  The expression of FOS protein in human glioma was strong. FOS protein probably plays a critical role in the progression of gliomas.
authors.affiliation: Department of Neurosurgery, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Tao
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Lu
authors.forename: Lei
authors.initials: L
authors.lastname: Yao
authors.forename: Jiajia
authors.initials: J
authors.lastname: Wang
authors.forename: Yan
authors.initials: Y
authors.lastname: Shi
authors.forename: Hui
authors.initials: H
authors.lastname: Luo
authors.forename: Ning
authors.initials: N
authors.lastname: Liu
authors.forename: Yongping
authors.initials: Y
authors.lastname: You
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005910:Glioma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism; D060787:Neoplasm Grading; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference
chemical_list: D016760:Proto-Oncogene Proteins c-fos; D019938:Cyclin D1; D020780:Matrix Metalloproteinase 9