title: Production of green fluorescent protein in transgenic rice seeds.
abstract: Immature embryos from immature seeds of rice (Oryza sativa L.) were transformed by biolistic bombardment with the plasmid carrying the coding region of the hygromycin phosphotransferase gene under the control of the 5' region of the cauliflower mosaic virus 35S promoter and the synthetic green fluorescence protein gene (sgfp) under the control of the maize ubiquitine promoter. Southern blot analysis confirmed the stable integration of hpt and sgfp genes in transformants. Subsequently leaves from regenerated plants were resistant to hygromycin, and microscopic observation of the green fluorescence and immunoblotting analysis revealed that green fluorescence protein was not only detected in the leaf and pollen of primary transformants but also in mature seeds. The results bear out the importance of the suitability of GFP as an in vivo marker to follow the processes of selection of somatic hybrid embryos and plants.
authors.affiliation: State Key Laboratory of Hybrid Rice, School of Longping, Central South University, Changsha 410125, China.
authors.forename: Ding
authors.initials: D
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Gao
authors.forename: Chunxiu
authors.initials: C
authors.lastname: Shen
authors.forename: Zhen
authors.initials: Z
authors.lastname: Fang
authors.forename: Yumei
authors.initials: Y
authors.lastname: Xia
authors.forename: Longping
authors.initials: L
authors.lastname: Yuan
authors.forename: Mengliang
authors.initials: M
authors.lastname: Cao
mesh_terms: D001483:Base Sequence; D019470:Biolistics; D015139:Blotting, Southern; D015153:Blotting, Western; D017931:DNA Primers; D049452:Green Fluorescent Proteins / Q000096:biosynthesis*; D008856:Microscopy, Fluorescence; D030821:Plants, Genetically Modified / Q000196:embryology*; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D012639:Seeds / Q000378:metabolism*
chemical_list: D017931:DNA Primers; D049452:Green Fluorescent Proteins