title: Ablation of Mina53 in mice reduces allergic response in the airways.
abstract: The mina53 (myc-induced nuclear antigen with a 53 kDa molecular mass; also known as mina) was identified as a direct transcriptional target of the oncoprotein Myc and encodes a conserved protein in vertebrates. While Mina53 is known to be associated with tumorigenesis, it is not clear what role Mina53 plays in non-neoplastic tissues. To directly address the roles of Mina53 in non-neoplastic tissues, we created mina53-deficient mice. Both male and female mina53-deficient mice reached adulthood and were fertile, suggesting that Mina53 is dispensable for the basic developmental processes. Since we found that Mina53 was expressed in cells responsible for immune responses, we investigated whether Mina53 was involved in immune responses. When mice were exposed intranasally to house dust mites as an allergen, the airway tract showed hyperresponsiveness to methacholine in wild-type mice but not in mina53-deficient mice. The mina53-deficient mice also showed a significantly reduced migration of immune cells, including eosinophils, into bronchoalveolar lavage fluid compared with wild-type mice. The levels of Th2 cytokines, IL-4 and IL-5, produced in response to house dust mites were lower in the mina53-deficient mice than in wild-type mice. The level of IFN-γ in bronchoalveolar lavage fluid was significantly decreased by exposure to house dust mites in wild-type mice but not in the mina53-deficient mice. These results suggest that Mina53 plays a role in the allergic response to inhaled allergens, possibly through controlling IL-4 production.
authors.affiliation: Laboratory of Allergy and Immunology, Faculty of Pharmacy, Takasaki University of Health and Welfare, Takasaki, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Mori
authors.forename: Kengo
authors.initials: K
authors.lastname: Okamoto
authors.forename: Yuji
authors.initials: Y
authors.lastname: Tanaka
authors.forename: Kwesi
authors.initials: K
authors.lastname: Teye
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Umata
authors.forename: Kinuko
authors.initials: K
authors.lastname: Ohneda
authors.forename: Kenichi
authors.initials: K
authors.lastname: Tokuyama
authors.forename: Masaru
authors.initials: M
authors.lastname: Okabe
authors.forename: Makoto
authors.initials: M
authors.lastname: Tsuneoka
mesh_terms: D000485:Allergens / Q000276:immunology; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002465:Cell Movement / Q000276:immunology; D004804:Eosinophils / Q000276:immunology; D005260:Female; D020397:Goblet Cells / Q000276:immunology; D007073:Immunoglobulin E / Q000097:blood; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D015848:Interleukin-5 / Q000378:metabolism; D008264:Macrophages / Q000276:immunology; D008297:Male; D016210:Methacholine Chloride / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008925:Mites / Q000276:immunology; D009363:Neoplasm Proteins / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism*; D009687:Nuclear Proteins / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism*; D012130:Respiratory Hypersensitivity / Q000276:immunology*
chemical_list: D000485:Allergens; D015848:Interleukin-5; C512207:Mina53 protein, mouse; D009363:Neoplasm Proteins; D009687:Nuclear Proteins; D016210:Methacholine Chloride; D015847:Interleukin-4; D007073:Immunoglobulin E; D007371:Interferon-gamma