title: Interaction between a cationic porphyrin and ctDNA investigated by SPR, CV and UV-vis spectroscopy.
abstract: The interaction between ctDNA and a cationic porphyrin was studied in this work. The binding process was monitored by surface plasmon resonance (SPR) spectroscopy in detail. The association, dissociation rate constants and the binding constants calculated by global analysis were 2.4×10(2)±26.4M(-1)s(-1), 0.011±0.0000056s(-1) and 2.18×10(4)M(-1), respectively. And the results were confirmed by cyclic voltammetry and UV-vis absorption spectroscopy. The binding constants obtained from cyclic voltammetry and UV-vis absorption spectroscopy were 8.28×10(4)M(-1) and 6.73×10(4)M(-1) at 298K, respectively. The covalent immobilization methodology of ctDNA onto gold surface modified with three different compounds was also investigated by SPR. These compounds all contain sulfydryl but with different terminated functional groups. The results indicated that the 11-MUA (HS(CH2)10COOH)-modified gold film is more suitable for studying the DNA-drug interaction.
authors.affiliation: State Key Laboratory of Virology & Key Laboratory of Analytical Chemistry for Biology and Medicine (MOE), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, PR China.
authors.forename: Zi-Qiang
authors.initials: ZQ
authors.lastname: Xu
authors.forename: Bo
authors.initials: B
authors.lastname: Zhou
authors.forename: Feng-Lei
authors.initials: FL
authors.lastname: Jiang
authors.forename: Jie
authors.initials: J
authors.lastname: Dai
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
mesh_terms: D000818:Animals; D002412:Cations / Q000737:chemistry; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D055664:Electrochemical Techniques; D011166:Porphyrins / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D020349:Surface Plasmon Resonance
chemical_list: D002412:Cations; D011166:Porphyrins; D004247:DNA; C427203:calf thymus DNA