title: Analysis of microarrays by MALDI-TOF MS.
abstract: Ligand libraries can be printed onto a sandwich composed of activated lipids embedded in a hydrophobic layer conjugated to an indium-tin oxide (ITO) surface. Arrays produced this way can be analyzed by fluorescence spectroscopy and mass spectrometry. Applications include the assignment of enzyme specificity, the profiling of glycoforms and the identification of lectins.
authors.affiliation: CICbiomaGUNE, Biofunctional Nanomaterials Unit, Paseo Miramon 182, 20009 San Sebastian, Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Beloqui
authors.forename: Javier
authors.initials: J
authors.lastname: Calvo
authors.forename: Sonia
authors.initials: S
authors.lastname: Serna
authors.forename: Shi
authors.initials: S
authors.lastname: Yan
authors.forename: Iain B H
authors.initials: IB
authors.lastname: Wilson
authors.forename: Manuel
authors.initials: M
authors.lastname: Martin-Lomas
authors.forename: Niels Christian
authors.initials: NC
authors.lastname: Reichardt
mesh_terms: D037102:Lectins / Q000032:analysis; D008055:Lipids / Q000737:chemistry; D040081:Protein Array Analysis; D040901:Proteomics; D013050:Spectrometry, Fluorescence; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013499:Surface Properties; D017971:Tin Compounds / Q000737:chemistry; D014166:Transferases / Q000378:metabolism
chemical_list: D037102:Lectins; D008055:Lipids; D017971:Tin Compounds; C109984:indium tin oxide; D014166:Transferases
keywords: lectins; mass spectrometry; microarrays; proteomics; transferases