title: Activation of intracellular phosphoinositide signaling after a single 600 nanosecond electric pulse.
abstract: Exposure to nanosecond pulsed electrical fields (nsPEFs) results in a myriad of observable effects in mammalian cells. While these effects are often attributed to the direct permeabilization of both the plasma and organelle membranes, the underlying mechanism(s) are not well understood. We hypothesize that nsPEF-induced membrane disturbance will initiate complex intracellular lipid signaling pathways, which ultimately lead to the observed multifarious effects. In this article, we show activation of one of these pathways--phosphoinositide signaling cascade. Here we demonstrate that nsPEF initiates phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) hydrolysis or depletion from the plasma membrane, accumulation of inositol-1,4,5-trisphosphate (IP3) in the cytoplasm and increase of diacylglycerol (DAG) on the inner surface of the plasma membrane. All of these events are initiated by a single 16.2 kV/cm, 600 ns pulse exposure. To further this claim, we show that the nsPEF-induced activation mirrors the response of M1-acetylcholine Gq/11-coupled metabotropic receptor (hM1). This demonstration of PIP2 hydrolysis by nsPEF exposure is an important step toward understanding the mechanisms underlying this unique stimulus for activation of lipid signaling pathways and is critical for determining the potential for nsPEFs to modulate mammalian cell functions.
authors.affiliation: National Research Council, Fort Sam Houston, TX, USA.
authors.forename: Gleb P
authors.initials: GP
authors.lastname: Tolstykh
authors.forename: Hope T
authors.initials: HT
authors.lastname: Beier
authors.forename: Caleb C
authors.initials: CC
authors.lastname: Roth
authors.forename: Gary L
authors.initials: GL
authors.lastname: Thompson
authors.forename: Jason A
authors.initials: JA
authors.lastname: Payne
authors.forename: Marjorie A
authors.initials: MA
authors.lastname: Kuipers
authors.forename: Bennett L
authors.initials: BL
authors.lastname: Ibey
mesh_terms: D000818:Animals; D002118:Calcium / Q000737:chemistry / Q000378:metabolism; D020169:Caspases / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism* / Q000528:radiation effects; D003593:Cytoplasm / Q000528:radiation effects; D004560:Electricity; D004574:Electromagnetic Fields; D006801:Humans; D019169:Jurkat Cells; D050356:Lipid Metabolism / Q000528:radiation effects; D010716:Phosphatidylinositols / Q000737:chemistry / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics / Q000528:radiation effects
chemical_list: D010716:Phosphatidylinositols; D020169:Caspases; D002118:Calcium
keywords: Electric pulse; PIP2 depletion; PKC and PLC; Phosphoinositide signaling; Plasma membrane nanopore