title: Aestuariihabitans beolgyonensis gen. nov., sp. nov., a novel alphaproteobacterium isolated from tidal flat sediment.
abstract: A Gram-negative, non-motile and rod-shaped bacterial strain, designated BB-MW15(T), was isolated from a tidal flat of the southern coast of the Korean peninsula, and its taxonomic position was investigated by using a polyphasic taxonomic approach. Strain BB-MW15(T) grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2-3 % (w/v) NaCl. Strain BB-MW15(T) exhibited the highest 16S rRNA gene sequence similarity values to the type strains of Marivita byunsanensis (96.8 %), Thalassobius maritimus (96.6 %), Sulfitobacter marinus (96.4 %) and Marinovum algicola (96.3 %). Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain BB-MW15(T) forms an independent lineage within the evolutionary radiation encompassed by the Roseobacter clade of the class Alphaproteobacteria. Strain BB-MW15(T) contained Q-10 as the predominant ubiquinone and C18:1 ω7c as the predominant fatty acid. The major polar lipids detected in strain BB-MW15(T) were phosphatidylcholine, phosphatidylglycerol, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain BB-MW15(T) is 62.7 mol%. Based on phylogenetic, chemotaxonomic and other phenotypic characteristics, strain BB-MW15(T) represents a new genus and a new species within Alphaproteobacteria, for which the name Aestuariihabitans beolgyonensis gen. nov., sp. nov. is proposed. The type strain of A. beolgyonensis is BB-MW15(T) (= KCTC 32324(T) = CCUG 63829(T)).
authors.affiliation: Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea. jhyoon69@skku.edu
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Yoon
authors.forename: Sooyeon
authors.initials: S
authors.lastname: Park
authors.forename: Yong-Taek
authors.initials: YT
authors.lastname: Jung
mesh_terms: D020561:Alphaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D012623:Seawater / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S