title: Opportunistic microorganisms in individuals with lesions of denture stomatitis.
abstract: The aim of this study was to isolate, quantify, identify, and compare opportunistic microorganisms (Candida and Staphylococcus genera and Enterobacteriaceae/Pseudomonadaceae families) from prosthesis-fitting surfaces, the hard palate, and mouth rinses of individuals wearing removable maxillary prosthesis with (50) and without (50) lesions of denture stomatitis (DS). The strains were collected and identified using phenotypic, biochemical and molecular tests. The counts of microorganisms were significantly higher in the group of individuals with DS (P < 0.05). C. albicans was the most frequently isolated yeast species in both groups, following by C. tropicalis and C. glabrata. Six isolates were identified as C. dubliniensis. S. aureus and S. epidermidis were the most frequent Staphylococcus species in both groups. Klebsiella pneumoniae was the predominant species in both groups. The association between Candida spp. and bacteria isolated in this study with DS suggests that these microorganisms may play important roles in the establishment and persistence of this disease.
authors.affiliation: Institute of Science and Technology, UNESP - Univ Estadual Paulista, São José dos Campos, São Paulo CEP: 12245-000, Brazil. cricabio@gmail.com
authors.forename: Cristiane Aparecida
authors.initials: CA
authors.lastname: Pereira
authors.forename: Bruna Costa
authors.initials: BC
authors.lastname: Toledo
authors.forename: Camila Teles
authors.initials: CT
authors.lastname: Santos
authors.forename: Anna Carolina Borges
authors.initials: AC
authors.lastname: Pereira Costa
authors.forename: Graziella Nuernberg
authors.initials: GN
authors.lastname: Back-Brito
authors.forename: Estela
authors.initials: E
authors.lastname: Kaminagakura
authors.forename: Antonio Olavo Cardoso
authors.initials: AO
authors.lastname: Jorge
mesh_terms: D000368:Aged; D015373:Bacterial Typing Techniques; D002175:Candida / Q000145:classification / Q000302:isolation & purification*; D002180:Candidiasis, Oral / Q000175:diagnosis / Q000209:etiology / Q000382:microbiology*; D016022:Case-Control Studies; D015169:Colony Count, Microbial; D003832:Denture, Partial, Removable / Q000009:adverse effects; D004755:Enterobacteriaceae / Q000145:classification / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009061:Mouth Mucosa / Q000382:microbiology / Q000473:pathology; D016533:Mycological Typing Techniques; D021362:Palate, Hard / Q000382:microbiology / Q000473:pathology; D011548:Pseudomonadaceae / Q000145:classification / Q000302:isolation & purification*; D013210:Staphylococcus / Q000145:classification / Q000302:isolation & purification*; D013282:Stomatitis, Denture / Q000175:diagnosis / Q000209:etiology / Q000382:microbiology*
keywords: Candida; Denture stomatitis; Enterobacteriaceae; Pseudomonadaceae; Staphylococcus