title: Detection of clonal KPC-2-producing Klebsiella pneumoniae ST258 in Korea during nationwide surveillance in 2011.
abstract: This study analysed the characteristics and genetic similarity of recent Klebsiella pneumoniae carbapenemase (KPC-2)-producing Klebsiella pneumoniae isolates from Korea. Recent laboratory surveillance detected an increase in carbapenemase-producing Enterobacteriaceae in Korea. A total of 6 KPC-2-producing K. pneumoniae were identified from 277 Enterobacteriaceae clinical isolates. All were sequence type (ST) 258 and they had the same pulsotype. They had high MICs for carbapenems and multi-drug resistance. TEM-1, SHV-11 and OXA type β-lactamases were detected in all isolates, whereas CTX-M type β-lactamases and plasmid-mediated AmpC β-lactamase (PABL) were not present. A conjugation experiment failed, but blaKPC-2-harbouring plasmids from the six isolates were used to transform Escherichia coli DH5-α by electroporation. Each of the transformants harboured a blaKPC-2-positive approximately 95 kb plasmid, which was typed in the IncFII incompatibility group and co-harboured TEM-1 and OXA-9 β-lactamases. They shared the same restriction profile. This study confirms the emergence of clonal ST258 KPC-2-producing K. pneumoniae in some regions of Korea.
authors.affiliation: Division of Antimicrobial Resistance, Korea National Institute of Health, 187 Osong Saengmyeong 2-ro Chungcheongbuk-do, 363-951, Republic of Korea.
authors.forename: Jung Sik
authors.initials: JS
authors.lastname: Yoo
authors.affiliation: Division of Antimicrobial Resistance, Korea National Institute of Health, 187 Osong Saengmyeong 2-ro Chungcheongbuk-do, 363-951, Republic of Korea.
authors.forename: Hye Mee
authors.initials: HM
authors.lastname: Kim
authors.affiliation: Division of Antimicrobial Resistance, Korea National Institute of Health, 187 Osong Saengmyeong 2-ro Chungcheongbuk-do, 363-951, Republic of Korea.
authors.forename: Jae Il
authors.initials: JI
authors.lastname: Yoo
authors.affiliation: Division of Antimicrobial Resistance, Korea National Institute of Health, 187 Osong Saengmyeong 2-ro Chungcheongbuk-do, 363-951, Republic of Korea.
authors.forename: Ji Woo
authors.initials: JW
authors.lastname: Yang
authors.affiliation: Division of Antimicrobial Resistance, Korea National Institute of Health, 187 Osong Saengmyeong 2-ro Chungcheongbuk-do, 363-951, Republic of Korea.
authors.forename: Hwa Su
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Division of Enteric Bacterial Infections, Korea National Institute of Health, 187 Osong Saengmyeong 2-ro Chungcheongbuk-do, 363-951, Republic of Korea.
authors.forename: Gyung Tae
authors.initials: GT
authors.lastname: Chung
authors.affiliation: Division of Antimicrobial Resistance, Korea National Institute of Health, 187 Osong Saengmyeong 2-ro Chungcheongbuk-do, 363-951, Republic of Korea.
authors.forename: Yeong Seon
authors.initials: YS
authors.lastname: Lee
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015780:Carbapenems / Q000494:pharmacology; D003227:Conjugation, Genetic; D024901:Drug Resistance, Multiple, Bacterial; D005838:Genotype; D006801:Humans; D007710:Klebsiella Infections / Q000175:diagnosis* / Q000201:enzymology / Q000453:epidemiology*; D007711:Klebsiella pneumoniae / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D015995:Prevalence; D056910:Republic of Korea / Q000453:epidemiology; D062606:Tertiary Care Centers; D001618:beta-Lactamases / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D000900:Anti-Bacterial Agents; D015780:Carbapenems; D001618:beta-Lactamases; C547585:beta-lactamase KPC-2, Klebsiella pneumoniae