title: Chemistry and biology of the potent endotoxin from a Burkholderia dolosa clinical isolate from a cystic fibrosis patient.
abstract: This is the first report of the chemical and biological properties of the lipooligosaccharide (LOS) endotoxin isolated from Burkholderia dolosa IST4208, an isolate recovered from a cystic fibrosis (CF) patient in a Portuguese CF center. B. dolosa is a member of the Burkholderia cepacia complex, a group of closely related species that are highly problematic and opportunistic pathogens in CF. B. dolosa infection leads to accelerated loss of lung function and decreased survival. The structural determination of its endotoxin was achieved using a combination of chemistry and spectroscopy, and has revealed a novel endotoxin structure. The purified LOS was tested for its immunostimulatory activity on human HEK 293 cells expressing TLR-4, MD-2, and CD-14. In these assays, the LOS showed strong proinflammatory activity.
authors.affiliation: Dipartimento di Scienze Chimiche, Università di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Napoli, Italy.
authors.forename: Flaviana Di
authors.initials: FD
authors.lastname: Lorenzo
authors.forename: Luisa
authors.initials: L
authors.lastname: Sturiale
authors.forename: Angelo
authors.initials: A
authors.lastname: Palmigiano
authors.forename: Luigi
authors.initials: L
authors.lastname: Lembo-Fazio
authors.forename: Ida
authors.initials: I
authors.lastname: Paciello
authors.forename: Carla P
authors.initials: CP
authors.lastname: Coutinho
authors.forename: Isabel
authors.initials: I
authors.lastname: Sá-Correia
authors.forename: MariaLina
authors.initials: M
authors.lastname: Bernardini
authors.forename: Rosa
authors.initials: R
authors.lastname: Lanzetta
authors.forename: Domenico
authors.initials: D
authors.lastname: Garozzo
authors.forename: Alba
authors.initials: A
authors.lastname: Silipo
authors.forename: Antonio
authors.initials: A
authors.lastname: Molinaro
mesh_terms: D000818:Animals; D042602:Burkholderia cepacia complex / Q000302:isolation & purification / Q000378:metabolism*; D003550:Cystic Fibrosis / Q000382:microbiology*; D016207:Cytokines / Q000378:metabolism; D004731:Endotoxins / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D005260:Female; D057809:HEK293 Cells; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000235:genetics / Q000378:metabolism; D008070:Lipopolysaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D051218:Lymphocyte Antigen 96 / Q000235:genetics / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000378:metabolism; D014162:Transfection
chemical_list: D016207:Cytokines; D004731:Endotoxins; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D051218:Lymphocyte Antigen 96; D051197:Toll-Like Receptor 4; C023023:lipid-linked oligosaccharides