title: Ultrafast, accurate, and robust localization of anisotropic dipoles.
abstract: The resolution of single molecule localization imaging techniques largely depends on the precision of localization algorithms. However, the commonly used Gaussian function is not appropriate for anisotropic dipoles because it is not the true point spread function. We derived the theoretical point spread function of tilted dipoles with restricted mobility and developed an algorithm based on an artificial neural network for estimating the localization, orientation and mobility of individual dipoles. Compared with fitting-based methods, our algorithm demonstrated ultrafast speed and higher accuracy, reduced sensitivity to defocusing, strong robustness and adaptability, making it an optimal choice for both two-dimensional and three-dimensional super-resolution imaging analysis.
authors.affiliation: College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
authors.forename: Yongdeng
authors.initials: Y
authors.lastname: Zhang
authors.forename: Lusheng
authors.initials: L
authors.lastname: Gu
authors.forename: Hao
authors.initials: H
authors.lastname: Chang
authors.forename: Wei
authors.initials: W
authors.lastname: Ji
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Mingshu
authors.initials: M
authors.lastname: Zhang
authors.forename: Lu
authors.initials: L
authors.lastname: Yang
authors.forename: Bei
authors.initials: B
authors.lastname: Liu
authors.forename: Liangyi
authors.initials: L
authors.lastname: Chen
authors.forename: Tao
authors.initials: T
authors.lastname: Xu
mesh_terms: D000429:Alcohol Oxidoreductases / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D000465:Algorithms; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D021621:Imaging, Three-Dimensional; D008856:Microscopy, Fluorescence; D016011:Normal Distribution; D010957:Plasmids / Q000378:metabolism
chemical_list: D003577:Cytochrome P-450 Enzyme System; D000429:Alcohol Oxidoreductases; C092153:microsomal ethanol-oxidizing system