title: In vivo interaction of gold nanoparticles after acute and chronic exposures in experimental animal models.
abstract: With emerging use of gold nanopaticles (GNP) in biomedical science now concern lies upon the fact that how this nonanparticles interact with biological systems both in vivo and in vitro. In this study effects of GNP (50 nm) were investigated in animal models after acute and chronic exposure. For acute studies GNP was administered intravenously at three doses and urine and blood samples were collected for urinary and haematological analysis at regular time intervals. For chronic studies GNP was administered intra-peritoneally at two dose levels and urine, blood, serum and tissue samples were collected for urinary, haematological, serum biochemical and histo-pathological analysis at regular intervals. Acute exposure revealed significant increase in WBC count at all the three dose levels and significant dose-dependent increase in RBC count and Hb%. Chronic exposure at 2 mg/kg dose level showed high toxicity. Significant changes in physical, morphological, WBC count and Hb% were observed after chronic exposure for multiple days. Histo-pathological studies indicated detrimental tissue histological changes in chronic animal models. Therefore, the above studies indicate that both acute and chronic GNP exposure exhibits potential physiological changes within animal system.
authors.affiliation: Laboratory of Toxinology and Experimental Pharmacodynamics, Department of Physiology, University of Calcutta, 92 A.PC. Road, Kolkata 700009, India.
authors.forename: Jayeeta
authors.initials: J
authors.lastname: Sengupta
authors.forename: Poulami
authors.initials: P
authors.lastname: Datta
authors.forename: Hirak Kr
authors.initials: HK
authors.lastname: Patra
authors.forename: Anjan Kr
authors.initials: AK
authors.lastname: Dasgupta
authors.forename: Antony
authors.initials: A
authors.lastname: Gomes
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D004906:Erythrocyte Count; D007958:Leukocyte Count; D008297:Male; D053768:Metal Nanoparticles / Q000633:toxicity*; D051379:Mice; D046529:Microscopy, Electron, Transmission; D023421:Models, Animal