title: Effects of porous beta-tricalcium phosphate-based ceramics used as an E. coli-derived rhBMP-2 carrier for bone regeneration.
abstract: Recombinant human bone morphogenetic protein-2 (rhBMP-2) requires carriers for clinical effectiveness. In this study, whether porous beta-tricalcium phosphate (β-TCP)-based ceramics are ideal carriers for rhBMP-2 was investigated. Hydroxyapatite (HA), β-TCP, TCP/HA (80 %/20 %), HA with rhBMP-2, TCP with rhBMP-2, and TCP/HA (80 %/20 %) with rhBMP-2 were manufactured by a sponge method with a pore size of 300 μm or more and macro-porosity of 83 %. The alkaline phosphatase (ALP) activity and ALP expression of the cells with 100 % β-TCP granules were more increased than the those of cells with 100 % HA and TCP/HA (80 %/20 %) at the baseline or when treated with 15 ng/ml of rhBMP-2. In an SD rat calvarial defect model, new bone formation was evidently shown in the TCP 100 %-rhBMP-2 and TCP/HA (80 %/20 %)-rhBMP-2 groups, showing that the most affected area was filled with newly-formed bone, that the percent bone volume and trabecular number were larger when compared to the groups without rhBMP-2 treatment at both 4 and 8 weeks after surgery using micro-CT and histology. Porous TCP-based ceramic granules enhanced the osteoblastic differentiation in the hMSC system when treated with 15 ng/ml of rhBMP-2 and accelerated bone-healing by trabecular number in a rat calvarial defect model. Thus, in this study it was proposed that TCP-based ceramics might be useful carriers of rhBMP-2.
authors.affiliation: Department of Orthopedic Surgery, College of Medicine, Seoul National University, SMG-SNU Boramae Medical Center, 425 Shindaebang-2-Dong, Seoul 156-707, Korea. spinelee@snu.ac.kr
authors.forename: Jae Hyup
authors.initials: JH
authors.lastname: Lee
authors.forename: Mi Young
authors.initials: MY
authors.lastname: Ryu
authors.forename: Hae-Ri
authors.initials: HR
authors.lastname: Baek
authors.forename: Kyung Mee
authors.initials: KM
authors.lastname: Lee
authors.forename: Jun-Hyuk
authors.initials: JH
authors.lastname: Seo
authors.forename: Hyun-Kyung
authors.initials: HK
authors.lastname: Lee
authors.forename: Hyun-Seung
authors.initials: HS
authors.lastname: Ryu
mesh_terms: D000469:Alkaline Phosphatase / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage* / Q000235:genetics; D001861:Bone Regeneration; D002130:Calcium Phosphates / Q000737:chemistry*; D002516:Ceramics; D024042:Collagen Type I / Q000235:genetics; D017931:DNA Primers; D004926:Escherichia coli / Q000235:genetics*; D066298:In Vitro Techniques; D016133:Polymerase Chain Reaction; D051381:Rats; D017207:Rats, Sprague-Dawley; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000008:administration & dosage* / Q000235:genetics; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D002130:Calcium Phosphates; D024042:Collagen Type I; D017931:DNA Primers; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C485817:beta-tricalcium phosphate; C499469:recombinant human bone morphogenetic protein-2; D000469:Alkaline Phosphatase