title: Beneficial effects of hyperosmotic perfusion in the myocardium after ischemia/reperfusion injury in isolated rat hearts.
abstract: OBJECTIVE
  A simple method to reduce the ischemia/reperfusion injury that can accompany cardiac surgery would have great clinical value. This study was to investigate the effect of hyperosmotic perfusion on ischemia/reperfusion injury in isolated perfused rat hearts.
  
  
  METHODS
  Forty male Sprague-Dawley rats were randomly divided either to have their isolated hearts perfused with normal osmotic buffer or buffer made hyperosmotic by addition of glucose. Hearts were then subjected to 30 min ischemia followed by 30 min reperfusion. Coronary flow, time to ischemic arrest, reperfusion arrhythmia, and ventricular function were recorded. Creatine phosphokinase leakage into the coronary artery, and myocardial content and activity of superoxide dismutase and catalase were also examined.
  
  
  RESULTS
  Rat hearts with hyperosmotic perfusion showed higher coronary flow, a prolonged time to ischemic arrest (10.60 vs. 5.63 min, P<0.005), a lower reperfusion arrthythmia score (3.2 vs. 5.3, P<0.001), better ventricular function, and less creatine phosphokinase leakage (340.1 vs. 861.9, P<0.001) than normal osmotic controls. Myocardial catalase content and activity were increased significantly (1435 vs. 917 U/g wet weight, P<0.001) in hearts perfused with hyperosmotic solution in comparison to the normal osmotic controls.
  
  
  CONCLUSIONS
  Pretreatment with hyperosmotic perfusion in normal rat hearts, which is attributed partly to the increased antioxidative activity, could provide beneficial effects from ischemia and reperfusion-induced injury by increasing coronary flow, and decreasing reperfusion arrhythmia.
authors.affiliation: No 6th People's Hospital and Department of Pharmacology, Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Cao
authors.forename: Lie
authors.initials: L
authors.lastname: Wang
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.forename: Zhiqian
authors.initials: Z
authors.lastname: Lv
mesh_terms: D000818:Animals; D015153:Blotting, Western; D003402:Creatine Kinase / Q000097:blood; D005947:Glucose / Q000008:administration & dosage / Q000737:chemistry; D006321:Heart / Q000503:physiopathology*; D006352:Heart Ventricles / Q000503:physiopathology; D008297:Male; D015428:Myocardial Reperfusion Injury / Q000097:blood / Q000517:prevention & control*; D019188:Organ Preservation Solutions / Q000008:administration & dosage* / Q000737:chemistry; D009994:Osmolar Concentration; D010477:Perfusion / Q000379:methods*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D013997:Time Factors; D016896:Treatment Outcome; D014325:Tromethamine / Q000008:administration & dosage / Q000737:chemistry
chemical_list: C074097:Krebs-Henseleit solution; D019188:Organ Preservation Solutions; D014325:Tromethamine; D003402:Creatine Kinase; D005947:Glucose