title: The influences of reaction conditions on magnetic porous pseudo-carbon paste electrode for schistosoma egg antigen detection.
abstract: A novel and sensitive electrochemical sensor based on magnetic porous pseudo-carbon paste electrode (MPPCPE) for schistosoma egg antigen (SEA) detection was described. The influences of immune reaction conditions such as time, pH and temperature on the detection current were investigated, and the influences of the blocking times of mercaptoethanol and ethanolamine on the background current were investigated. The experimental results shown that the high detection current and low background current could be obtained when the immune reaction temperature was 30 degrees C, the immune reaction time was 40 min, the immune reaction pH was 7.2, the blocking time of ethanolamine was 40 min, and the blocking time of mercaptoethanol was 60 min. At the optimum reaction conditions, a limit of detecting SEA as low as 0.01 microg/mL was obtained using MPPCPE.
authors.affiliation: Hunan Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou 412007, PR China.
authors.forename: Lijian
authors.initials: L
authors.lastname: Xu
authors.forename: Jingjing
authors.initials: J
authors.lastname: Du
authors.forename: Nongyue
authors.initials: N
authors.lastname: He
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000032:analysis*; D002244:Carbon; D004566:Electrodes; D019856:Ethanolamine / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D008280:Magnetics; D008623:Mercaptoethanol / Q000494:pharmacology; D012547:Schistosoma / Q000187:drug effects / Q000276:immunology*; D013696:Temperature
chemical_list: D000947:Antigens, Helminth; D019856:Ethanolamine; D008623:Mercaptoethanol; D002244:Carbon