title: Methanogenic activities of sulfide and nitric oxide amended mesophilic, methanogenic culture: role of nitrososufides complex.
abstract: Sulfide (S(2-)) and nitric oxide (NO) can be formed in anaerobic digester if nitrate and sulfate were presented in the influent streams. This study noted by 4,5-diaminofluorescein diacetate (DAF-2DA) staining and confocal laser scanning microscopy (CLSM) the presence of nitrososulfides (SNO) complex in the S(2-)+NO-amended methanogenic sludge. Kinetic analysis suggested two-step kinetics involving chemical equilibrium between S(2-), NO and SNO as step 1 and the slow conversion from SNO to N2O as step 2. The SNO complex was claimed to have higher inhibition effect than S(2-) or NO alone on the methangenic activities. Comments were made on the role of SNO complex for the interactions between sulfur, nitrogen and carbon metabolisms in anaerobic digestion.
authors.affiliation: Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan. djlee@ntu.edu.tw
authors.forename: Duu-Jong
authors.initials: DJ
authors.lastname: Lee
authors.forename: Biing-Teo
authors.initials: BT
authors.lastname: Wong
mesh_terms: D000693:Anaerobiosis; D019605:Euryarchaeota / Q000378:metabolism*; D007700:Kinetics; D008697:Methane / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D013440:Sulfides / Q000378:metabolism*
chemical_list: D013440:Sulfides; D009569:Nitric Oxide; D008697:Methane
keywords: Anaerobic digestion; CLSM; Denitrifying sulfide removal; Inhibition