title: The secretome signature of colon cancer cell lines.
abstract: The definition of the secretome signature of a cancer cell line can be considered a potential tool to investigate tumor aggressiveness and a preclinical exploratory study required to optimize the search of cancer biomarkers. Dealing with a cell-specific secretome limits the contamination by the major components of the human serum and reduces the range of dynamic concentrations among the secreted proteins, thus favouring under-represented tissue-specific species. The aim of the present study is to characterize the secretome of two human colon carcinoma cell lines, CaCo-2 and HCT-GEO, in order to evaluate differences and similarities of two colorectal cancer model systems. In this study, we identified more than 170 protein species, 64 more expressed in the secretome of CaCo-2 cells and 54 more expressed in the secretome of HCT-GEO cells; 58 proteins were shared by the two systems. Among them, more than 50% were deemed to be secretory according to their Gene Ontology annotation and/or to their SignalP or SecretomeP scores. Such a characterization allowed corroborating the potential of a cell culture-based model in order to describe the cell-specific invasive properties and to provide a list of putative cancer biomarkers.
authors.affiliation: Fondazione IRCCS SDN, Naples, Italy.
authors.forename: Esther
authors.initials: E
authors.lastname: Imperlini
authors.forename: Irene
authors.initials: I
authors.lastname: Colavita
authors.forename: Marianna
authors.initials: M
authors.lastname: Caterino
authors.forename: Peppino
authors.initials: P
authors.lastname: Mirabelli
authors.forename: Daniela
authors.initials: D
authors.lastname: Pagnozzi
authors.forename: Luigi
authors.initials: L
authors.lastname: Del Vecchio
authors.forename: Rosa
authors.initials: R
authors.lastname: Di Noto
authors.forename: Margherita
authors.initials: M
authors.lastname: Ruoppolo
authors.forename: Stefania
authors.initials: S
authors.lastname: Orrù
mesh_terms: D015153:Blotting, Western; D018938:Caco-2 Cells; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D040901:Proteomics; D053719:Tandem Mass Spectrometry
keywords: CACO-2; COLON CANCER; HCT-GEO; PROTEOMICS; SECRETOME