title: Top-down chemoenzymatic approach to a high-mannose-type glycan library: synthesis of a common precursor and its enzymatic trimming.
abstract: From the stacks: A novel method for construction of a high-mannose-type glycan library by systematic enzymatic trimming of a single synthetic Man9-based precursor was developed. Efficient chemical synthesis of the tetradecasaccharide common precursor and orthogonal enzymatic trimming to obtain all M(8-9) and G(1)M(8-9) derivatives was demonstrated. G = glucose, M = mannose.
authors.affiliation: ERATO, Ito Glycotrilogy Project (Japan) Science and Technology Agency, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Koizumi
authors.forename: Ichiro
authors.initials: I
authors.lastname: Matsuo
authors.forename: Maki
authors.initials: M
authors.lastname: Takatani
authors.forename: Akira
authors.initials: A
authors.lastname: Seko
authors.forename: Masakazu
authors.initials: M
authors.lastname: Hachisu
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Takeda
authors.forename: Yukishige
authors.initials: Y
authors.lastname: Ito
mesh_terms: D001896:Boron Compounds / Q000737:chemistry; D002240:Carbohydrate Sequence; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000378:metabolism; D008358:Mannose / Q000737:chemistry*; D008969:Molecular Sequence Data; D011134:Polysaccharides / Q000138:chemical synthesis / Q000737:chemistry*; D017510:Protein Folding
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D006023:Glycoproteins; D011134:Polysaccharides; D006026:Glycoside Hydrolases; D008358:Mannose
keywords: carbohydrates; enzymes; glycopeptides; protein folding; synthesis design