title: Effects of magnoline on P-selectin's expression in diabetic rats and its reno-protection.
abstract: OBJECTIVE
  Magnoline is an active ingredient of magnolia fargesii with anti-inflammatory and anti-platelet effects. The objective is to explore the renoprotection of magnoline in diabetic rats and its effects on P-selectin.
  
  
  METHODS
  Thirty-six rats were randomized into 4 groups-normal control group (C), diabetic group (D), small-dose magnoline treatment group (M1) and large-dose magnoline treatment group (M2) (n=9 in each group). Streptozotocin was selected to construct diabetic rat model, and group M1 and group M2 were treated with magnoline 0.5mg/Kg.d and 2mg/Kg.d respectively. Urinary albumin excretion rate, renal function, levels of P-selectin and TGF-β1 were observed after 16 weeks.
  
  
  RESULTS
  Levels of albuminuria and serum creatinine of group M1 (1078.9 ± 77.3μg/24h, 29.7 ± 3.9μmol/L) and M2 (852.9 ± 80.1μg/24h, 30.9 ± 2.9μmol/L) were lower than group D (1572.8 ± 176.2μg/24h, 39.4 ± 4.1μmol/L) (P <0.05). Serum levels of P-selectin in group M1 and M2 were lower than group D (P <0.05). The renal expression of P-selectin and TGF-β1 in group M1 and M2 were significantly attenuated respectively.
  
  
  CONCLUSIONS
  Magnoline has reno-protective effects on diabetic rats which may be related to the inhibition of P-selectin.
authors.affiliation: Department of Nephrology, The First Affiliated Hospital of Harbin Medical University, No.23 Youzheng Street, Nangang District, Harbin 150001, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Lirong
authors.initials: L
authors.lastname: Hao
authors.forename: Niansong
authors.initials: N
authors.lastname: Wang
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D002097:C-Reactive Protein / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D003928:Diabetic Nephropathies / Q000473:pathology / Q000517:prevention & control*; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis; D007546:Isoquinolines / Q000494:pharmacology*; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D007677:Kidney Function Tests; D050356:Lipid Metabolism / Q000187:drug effects; D031566:Magnolia / Q000737:chemistry*; D008297:Male; D009929:Organ Size; D019007:P-Selectin / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D010936:Plant Extracts / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis
chemical_list: D001786:Blood Glucose; D007546:Isoquinolines; D019007:P-Selectin; D010936:Plant Extracts; D053773:Transforming Growth Factor beta1; C585647:magnoline; D018799:Intercellular Adhesion Molecule-1; D002097:C-Reactive Protein