title: Styrene altered clock gene expression in serum-shocked cultured human fibroblasts.
abstract: The circadian clock can regulate the metabolic process of xenobiotics, but little is known as to circadian rhythms can be perturbed by xenobiotics. Styrene is a organic chemical widely used in occupational settings. The effects of styrene on the circadian genes of HuDE cells were evaluated after serum-shocking synchronization. A subtoxic dose of 100 µM of styrene altered the expression of clock genes BMAL1, PER2, PER3, CRY1, CRY2, and REV-ERB-α.
authors.affiliation: Department of Clinical and Molecular Sciences, Polytechnic University of Marche, Ancona, Italy.
authors.forename: Nicola
authors.initials: N
authors.lastname: Manzella
authors.forename: Massimo
authors.initials: M
authors.lastname: Bracci
authors.forename: Sara
authors.initials: S
authors.lastname: Staffolani
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Strafella
authors.forename: Venerando
authors.initials: V
authors.lastname: Rapisarda
authors.forename: Matteo
authors.initials: M
authors.lastname: Valentino
authors.forename: Monica
authors.initials: M
authors.lastname: Amati
authors.forename: Alfredo
authors.initials: A
authors.lastname: Copertaro
authors.forename: Lory
authors.initials: L
authors.lastname: Santarelli
mesh_terms: D056930:ARNTL Transcription Factors / Q000096:biosynthesis; D002478:Cells, Cultured; D002940:Circadian Rhythm / Q000187:drug effects* / Q000235:genetics; D056931:Cryptochromes / Q000096:biosynthesis; D005347:Fibroblasts / Q000187:drug effects*; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D006801:Humans; D056953:Nuclear Receptor Subfamily 1, Group D, Member 1 / Q000096:biosynthesis; D056950:Period Circadian Proteins / Q000096:biosynthesis; D020058:Styrene / Q000008:administration & dosage*; D015262:Xenobiotics / Q000008:administration & dosage
chemical_list: D056930:ARNTL Transcription Factors; C538960:BMAL1 protein, human; C538963:CRY1 protein, human; C538966:CRY2 protein, human; D056931:Cryptochromes; C064353:NR1D1 protein, human; D056953:Nuclear Receptor Subfamily 1, Group D, Member 1; C421902:PER2 protein, human; C512644:PER3 protein, human; D056950:Period Circadian Proteins; D015262:Xenobiotics; D020058:Styrene