title: Study of angiogenic gene ointments designed for skin neovascularization.
abstract: OBJECTIVE
  In this study attention was focused on gene preparations that stimulate angiogenesis in the skin. Angiogenesis was stimulated by gene preparations encoding angiogenic factors introduced into the skin by injection, and applied as ointments.
  
  
  METHODS
  The appropriate angiogenic formulations containing angiogenic nonviral vectors (pVEGF, pFGF, pSDF, pVIF), or viral vectors (rAAV/VEGF, rAAV/SDF) were prepared for the test. Cholesterol ointment was used as the vehicle for viral and non-viral vectors. The new vessels in the mouse skin were counted according to the criteria suggested by Sidky and Auerbach.
  
  
  RESULTS
  Studies indicate that all non-viral (pVEGF, pFGF, pSDF, pVIF) and viral (rAAV/VEGF, rAAV/SDF) vectors strongly stimulate new vessel formation when administered into mouse skin as injections. The impact of angiogenic gene ointments for skin angiogenesis was about 3-4 times weaker than that observed for injection preparations.
  
  
  CONCLUSIONS
  Angiogenic injection gene preparations strongly stimulate skin neovascularization. The clinical usefulness of gene ointments should stimulate further laboratory studies in the field of experimental skin gene therapy.
authors.affiliation: Department of Molecular Biology, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Karolina
authors.initials: K
authors.lastname: Hajdukiewicz
authors.forename: Anna
authors.initials: A
authors.lastname: Stachurska
authors.forename: Robert
authors.initials: R
authors.lastname: Proczka
authors.forename: Maciej
authors.initials: M
authors.lastname: Małecki
mesh_terms: D000279:Administration, Cutaneous; D043925:Angiogenesis Inducing Agents / Q000008:administration & dosage*; D042501:Angiogenic Proteins / Q000008:administration & dosage*; D000818:Animals; D015316:Genetic Therapy; D005822:Genetic Vectors / Q000008:administration & dosage; D007271:Injections, Intradermal; D051379:Mice; D008807:Mice, Inbred BALB C; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D009824:Ointments; D012867:Skin / Q000098:blood supply*
chemical_list: D043925:Angiogenesis Inducing Agents; D042501:Angiogenic Proteins; D009824:Ointments