title: Improved direct electrochemistry for proteins adsorbed on a UV/ozone-treated carbon nanofiber electrode.
abstract: We studied the direct electron transfer (DET) of proteins on a carbon nanofiber (CNF) modified carbon film electrode by employing the one-step UV/ozone treatment of CNF. This treatment changed the CNF surface from hydrophobic to hydrophilic because a sufficient quantity of oxygen functional groups was introduced onto the CNF surface. Furthermore, this simple approach increased both the effective surface area and the number of edge-plane defect sites. As a result, the reversibility of redox species, such as ferrocyanide and dopamine, was greatly improved on the treated electrode surface. We obtained on efficient DET of bilirubin oxidase (BOD) and cytochrome c (cyt c) at the treated CNF electrode, which exhibited 38 (for BOD) and 6 (for cyt c) times higher than that at untreated CNF modified electrode. These results indicate that the combination of nanostructured carbon and this UV/ozone treatment process can efficiently create a functionalized surface for the electron transfer of proteins.
authors.affiliation: National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Xue
authors.forename: Dai
authors.initials: D
authors.lastname: Kato
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Kamata
authors.forename: Qiaohui
authors.initials: Q
authors.lastname: Guo
authors.forename: Tianyan
authors.initials: T
authors.lastname: You
authors.forename: Osamu
authors.initials: O
authors.lastname: Niwa
mesh_terms: D000327:Adsorption; D002244:Carbon / Q000737:chemistry*; D045304:Cytochromes c / Q000737:chemistry; D004298:Dopamine / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D005295:Ferrocyanides / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry*; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000737:chemistry; D010126:Ozone / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D013499:Surface Properties; D014466:Ultraviolet Rays
chemical_list: D005295:Ferrocyanides; D011506:Proteins; D010126:Ozone; D002244:Carbon; D045304:Cytochromes c; D044925:Oxidoreductases Acting on CH-CH Group Donors; C042401:bilirubin oxidase; C020354:hexacyanoferrate II; D004298:Dopamine