title: Expression of resistin, CXCR3, IP-10, CCR5 and MIP-1α in obese patients with different severity of asthma.
abstract: Asthma studies suggest that alteration in the inflammation pattern may be associated with the severity of asthma. The aim of this study was to compare in vitro the expression of chemokines, chemokine receptors and cytokine production from CD4+ T human lymphocytes of asthmatic, both obese and non-obese patients with different severity levels of asthma. Lymphocytes were labeled with monoclonal anti-human CXCR3/IP-10, MIP-1a/CCR5 antibodies and were analyzed by flow cytometry. Cell culture supernatants were used to measure production of interleukin IL-6 and resistin by ELISA. CXCR3/IP-10 expression increased in non-obese patients with mild persistent asthma (2.2%, p<0.05), moderate persistent asthma (3%, p<0.003) and severe persistent asthma (4%, p<0.004); this effect was stronger in obese patients with severe persistent asthma (35%, p<0.004). MIP-1 α / CCR5 increased in non-obese patients with intermittent asthma (0.65%, p<0.05) and severe asthma (1.4%, p<0.03); in obese patients, this expression was greater in intermittent asthma (8%, p<0.05) and severe persistent asthma (12%, p<0.04). Resistin production strongly increased in obese patients with intermittent (976 ng/ml) and severe persistent asthma (795 ng/ml). IL-6 increased in both lean and obese persons; however, the highest value was registered in the group of severe persistent obese asthmatics (992 pg/ml). Obesity per se increased the inflammatory profile of chemokines / cytokines secreted by cells of the blood, increasing the inflammatory status in asthmatic patients. Resistin showed characteristics of a pro-inflammatory cytokine mainly in severely obese asthmatics.
authors.affiliation: Unidad de Investigación Médica en Inmunología, Hospital de Pediatría, Centro Medico Nacional SXXI, Instituto Mexicano del Seguro Social, México D.F., México. srdotor@yahoo.com.mx
authors.forename: Sara
authors.initials: S
authors.lastname: Rojas-Dotor
authors.forename: Nora H
authors.initials: NH
authors.lastname: Segura-Méndez
authors.forename: Ken
authors.initials: K
authors.lastname: Miyagui-Namikawa
authors.forename: Rafael
authors.initials: R
authors.lastname: Mondragón-González
mesh_terms: D001249:Asthma / Q000097:blood* / Q000150:complications; D015992:Body Mass Index; D015496:CD4-Positive T-Lymphocytes / Q000502:physiology; D016022:Case-Control Studies; D054405:Chemokine CCL3 / Q000097:blood*; D054357:Chemokine CXCL10 / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D009765:Obesity / Q000097:blood* / Q000150:complications; D061251:Primary Cell Culture; D019713:Receptors, CCR5 / Q000097:blood; D054367:Receptors, CXCR3 / Q000097:blood; D019707:Receptors, Chemokine / Q000097:blood*; D052243:Resistin / Q000097:blood*; D012720:Severity of Illness Index; D018709:Statistics, Nonparametric
chemical_list: D054405:Chemokine CCL3; D054357:Chemokine CXCL10; D015850:Interleukin-6; D019713:Receptors, CCR5; D054367:Receptors, CXCR3; D019707:Receptors, Chemokine; D052243:Resistin