title: Complete sequences of two plasmids in a blaNDM-1-positive Klebsiella oxytoca isolate from Taiwan.
abstract: Genetic determinants of a bla(NDM-1)-positive, multidrug-resistant bacterial isolate that caused active infection was investigated by DNA sequencing. Two plasmids, pKOX_NDM1 and pKOX-R1, were identified for the Klebsiella oxytoca strain E718. Sequence annotation revealed a bla(NDM-1) gene in pKOX_NDM1 and two extended-spectrum β-lactamase producers (bla(CTX-M-3) and blaSHV-12) and a wide array of resistance genes in pKOX-R1. These findings highlight the difficulty in treating multidrug-resistant bacterial infections and the potential danger of emerging resistant enterobacteria.
authors.affiliation: Institute of Molecular and Genomic Medicine, National Health Research Institutes, Zhunan, Taiwan.
authors.forename: Tzu-Wen
authors.initials: TW
authors.lastname: Huang
authors.forename: Jann-Tay
authors.initials: JT
authors.lastname: Wang
authors.forename: Tsai-Ling
authors.initials: TL
authors.lastname: Lauderdale
authors.forename: Tsai-Lien
authors.initials: TL
authors.lastname: Liao
authors.forename: Jui-Fen
authors.initials: JF
authors.lastname: Lai
authors.forename: Mei-Chen
authors.initials: MC
authors.lastname: Tan
authors.forename: Ann-Chi
authors.initials: AC
authors.lastname: Lin
authors.forename: Ying-Tsong
authors.initials: YT
authors.lastname: Chen
authors.forename: Shih-Feng
authors.initials: SF
authors.lastname: Tsai
authors.forename: Shan-Chwen
authors.initials: SC
authors.lastname: Chang
mesh_terms: D000617:Aminoglycosides / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003227:Conjugation, Genetic; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005798:Genes, Bacterial; D019450:Genes, MDR; D006801:Humans; D007710:Klebsiella Infections / Q000175:diagnosis; D041121:Klebsiella oxytoca / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics*; D013624:Taiwan; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; C417271:beta-lactamase CTX-M-3; C107198:beta-lactamase SHV-12; D001618:beta-Lactamases; C552864:beta-lactamase NDM-1