title: ROCK is involved in vimentin phosphorylation and rearrangement induced by dengue virus.
abstract: Our previous study showed that dengue virus 2 (DENV2) infection induces rearrangement of vimentin into dense structures at the perinuclear area. However, the underlying mechanism of this phenomenon is poorly characterized. In the present work, we found that vimentin and Ser71 phosphorylated vimentin display similar distributions in DENV2-infected cells. DENV2 infection also induced ROCK activation and phosphorylation of vimentin at Ser71 as the DENV2 infection progressed. Furthermore, Ser71 phosphorylation and vimentin rearrangement induced by DENV2 infection were blocked by the ROCK inhibitor Y-27632. In addition, DENV2 led to endoplasmic reticulum (ER) redistribution in the perinuclear region of the host cells, which was partially blocked by pretreatment with Y-27632. Together, these data support indicate that ROCK may have a role in governing regulating vimentin and ER rearrangement during DENV2 infection. We hypothesize that DENV2 infection, via ROCK activation, induces both vimentin rearrangement and ER redistribution around the perinuclear region, which may play a structural role in anchoring DENV2 to replication sites.
authors.affiliation: Department of Microbiology, Third Military Medical University, Chongqing, 400038, People's Republic of China.
authors.forename: Shun
authors.initials: S
authors.lastname: Lei
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Tian
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Xiao
authors.forename: Shu
authors.initials: S
authors.lastname: Li
authors.forename: Xian-Cai
authors.initials: XC
authors.lastname: Rao
authors.forename: Jun-Lei
authors.initials: JL
authors.lastname: Zhang
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Hu
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
mesh_terms: D000577:Amides / Q000494:pharmacology; D002460:Cell Line; D003716:Dengue Virus / Q000502:physiology*; D004721:Endoplasmic Reticulum / Q000187:drug effects / Q000378:metabolism / Q000821:virology; D006801:Humans; D010766:Phosphorylation / Q000187:drug effects; D011725:Pyridines / Q000494:pharmacology; D014746:Vimentin / Q000378:metabolism*; D014779:Virus Replication; D054460:rho-Associated Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D000577:Amides; D011725:Pyridines; D014746:Vimentin; C108830:Y 27632; D054460:rho-Associated Kinases