title: Evaluation of three RT-qPCR-based miRNA detection methods using seven rice miRNAs.
abstract: Three frequently-used reverse transcription-quantitative polymerase chain reaction (RT-qPCR)-based miRNA detection methods, stem-loop RT-qPCR, poly(A)-tailing RT-qPCR, and miQPCR, were evaluated using seven selected rice miRNAs. The results revealed that miRNA abundance and sequence characteristics can affect capability of detection. The stem-loop amplification technique detected highly and moderately abundant miRNAs. The poly(A)-tailing method detected both highly abundant and sparsely present miRNAs, but failed to detect miRNAs with a hairpin structure. Only a few miRNAs were detectable by the miQPCR method. We suggest that a combination of methods should be used for reliable quantitative investigation of miRNAs.
authors.affiliation: College of Natural Resources and Environment, South China Agricultural University, Guangzhou, PR China.
authors.forename: Guiping
authors.initials: G
authors.lastname: Mou
authors.forename: Kang
authors.initials: K
authors.lastname: Wang
authors.forename: Donglin
authors.initials: D
authors.lastname: Xu
authors.forename: Guohui
authors.initials: G
authors.lastname: Zhou
mesh_terms: D020869:Gene Expression Profiling; D035683:MicroRNAs / Q000235:genetics / Q000302:isolation & purification*; D012275:Oryza / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*
chemical_list: D035683:MicroRNAs