title: Establishment of monoclonal antibodies against the extracellular domain that block binding of NMO-IgG to AQP4.
abstract: Neuromyelitis optica is a demyelinating disease characterized by a disease-specific autoantibody designated as NMO-IgG that specifically recognizes aquaporin-4, and the binding of NMO-IgG to AQP4 causes the progress of the disease. Prevention of the binding of NMO-IgG, therefore, may alleviate the disease. Here we have developed monoclonal antibodies against AQP4 with a baculovirus display system in order to obtain high affinity monoclonal antibodies against the extracellular domains of AQP4. Our monoclonal antibodies can block the binding of NMO-IgG in spite of their heterogeneity. Taken together, we propose that our monoclonal antibodies can be applied in clinical therapy for NMO patients.
authors.affiliation: Department of Pharmacology, School of Medicine, Keio University, 35 Shinanomachi, Shinjyuku-ku, Tokyo 160-8582, Japan.
authors.forename: Kaori
authors.initials: K
authors.lastname: Miyazaki
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Abe
authors.forename: Hiroko
authors.initials: H
authors.lastname: Iwanari
authors.forename: Yota
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Takahiro
authors.initials: T
authors.lastname: Kikuchi
authors.forename: Takashi
authors.initials: T
authors.lastname: Ito
authors.forename: Jungo
authors.initials: J
authors.lastname: Kato
authors.forename: Osamu
authors.initials: O
authors.lastname: Kusano-Arai
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Takahashi
authors.forename: Shuhei
authors.initials: S
authors.lastname: Nishiyama
authors.forename: Hiroko
authors.initials: H
authors.lastname: Ikeshima-Kataoka
authors.forename: Shoji
authors.initials: S
authors.lastname: Tsuji
authors.forename: Takeshi
authors.initials: T
authors.lastname: Arimitsu
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kato
authors.forename: Toshiko
authors.initials: T
authors.lastname: Sakihama
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Toyama
authors.forename: Kazuo
authors.initials: K
authors.lastname: Fujihara
authors.forename: Takao
authors.initials: T
authors.lastname: Hamakubo
authors.forename: Masato
authors.initials: M
authors.lastname: Yasui
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000627:therapeutic use*; D051401:Aquaporin 4 / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D001323:Autoantibodies / Q000737:chemistry / Q000276:immunology; D016466:CHO Cells; D006224:Cricetinae; D005434:Flow Cytometry; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry / Q000276:immunology*; D008969:Molecular Sequence Data; D009471:Neuromyelitis Optica / Q000188:drug therapy* / Q000276:immunology*; D009865:Oocytes / Q000166:cytology; D011485:Protein Binding / Q000276:immunology; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D014981:Xenopus
chemical_list: C496030:AQP4 protein, human; D000911:Antibodies, Monoclonal; D051401:Aquaporin 4; D001323:Autoantibodies; D007074:Immunoglobulin G; D011994:Recombinant Proteins