title: A human thyroid cancer cell line, DH-14-3, newly established from poorly differentiated thyroid carcinoma.
abstract: Poorly differentiated thyroid carcinoma (PDTC) is a newly recognized histological type of malignant thyroid tumor, accounting for about 2 - 13% of all thyroid carcinomas. PDTC is considered as a morphologically and biologically intermediate stage between well-differentiated thyroid carcinoma and anaplastic thyroid carcinoma. PDTC preferentially manifests bone metastases. We here established a cell line from a resected tumor specimen from a 70-year-old male patient with PDTC who presented with multiple bone metastases. This new thyroid tumor cell line was designated as DH-14-3 and was subsequently grown in culture for several years. DH-14-3 cells express thyroglobulin in the cytoplasm and thyroid transcription factor-1 in the nuclei, both proteins of which are specific markers for the thyroid gland. Importantly, triiodothyronine (T3) was detected in the cultured medium of DH-14-3 cells, in which, however, thyroxine (T4) was undetectable. Moreover, DH-14-3 cells secreted interleukin-8, transforming growth factor-β1, vascular endothelial growth factor, matrix metalloproteinase-1 and parathyroid hormone-related protein, all of which may be responsible for the aggressiveness or bone metastasis of PDTC. Thus, the production of these proteins may reflect the metastatic potential of this cell line. DH-14-3 cells also express CXC chemokine receptor-4 and epidermal growth factor receptor, and carry a missense mutation in the p53 tumor suppressor gene. In fact, transplantation of DH-14-3 cells into the back of nude mice resulted in the formation of tumors, thereby confirming the capability of tumorigenesis. DH-14-3 cells may be useful for investigating the biological features of PDTC and will contribute to the therapeutic study of thyroid cancer.
authors.affiliation: Department of Advanced Surgical Science and Technology, Tohoku University Graduate School of Medicine, Miyagi, Japan. jintesshy@yahoo.co.jp
authors.forename: Jin
authors.initials: J
authors.lastname: Teshima
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Doi
authors.forename: Keisei
authors.initials: K
authors.lastname: Fujimori
authors.forename: Michio
authors.initials: M
authors.lastname: Watanabe
authors.forename: Noriaki
authors.initials: N
authors.lastname: Nakajima
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Nakano
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Noriaki
authors.initials: N
authors.lastname: Ohuchi
authors.forename: Susumu
authors.initials: S
authors.lastname: Satomi
mesh_terms: D000368:Aged; D000818:Animals; D001859:Bone Neoplasms / Q000556:secondary; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D051379:Mice; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D044162:Parathyroid Hormone-Related Protein / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D013964:Thyroid Neoplasms / Q000473:pathology*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: C508606:CXCL8 protein, human; D016209:Interleukin-8; D044162:Parathyroid Hormone-Related Protein; C508960:TGFB1 protein, human; C495901:TP53 protein, human; D053773:Transforming Growth Factor beta1; D016159:Tumor Suppressor Protein p53; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1