title: Leaf extract of Azadirachta indica (neem): a potential antibiofilm agent for Pseudomonas aeruginosa.
abstract: Pseudomonas aeruginosa is well known for its ability to form biofilm on indwelling medical devices. These biofilms are difficult to remove because of their high tolerance to conventional antibiotics. Therefore, there is a need to look for alternative agents such as medicinal plants, which can eradicate or inhibit biofilm effectively. This study evaluated the role of neem in inhibiting biofilm formation by P aeruginosa Factors contributing to adherence and biofilm formation were also studied. Results demonstrated that neem leaves extract was quite effective in disrupting formation and structure of biofilms. Moreover, the level of exopolysaccharide, alginate, hydrophobic interactions and uroepithelial cell attachment, which contributes to biofilm formation, was also affected significantly. Results confirm the effectiveness of neem extract in inhibiting biofilm formation. Such studies can lead to the discovery of safe antimicrobial drugs from natural sources without the risk of resistance.
authors.affiliation: Department of Microbiology, BMS Block, Punjab University, Chandigarh, India kusum_harjai@hotmail.com.
authors.forename: Kusum
authors.initials: K
authors.lastname: Harjai
authors.affiliation: Department of Microbiology, BMS Block, Punjab University, Chandigarh, India.
authors.forename: Anju
authors.initials: A
authors.lastname: Bala
authors.affiliation: Department of Microbiology, BMS Block, Punjab University, Chandigarh, India.
authors.forename: Ravi K
authors.initials: RK
authors.lastname: Gupta
authors.affiliation: Department of Microbiology, BMS Block, Punjab University, Chandigarh, India.
authors.forename: Radhika
authors.initials: R
authors.lastname: Sharma
mesh_terms: D018829:Adhesins, Bacterial / Q000378:metabolism; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology*; D031601:Azadirachta / Q000737:chemistry*; D001422:Bacterial Adhesion / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000502:physiology
chemical_list: D018829:Adhesins, Bacterial; D000900:Anti-Bacterial Agents; D010936:Plant Extracts
keywords: Azadirachta indica (neem); Pseudomonas aeruginosa; biofilms; scanning electron microscope