title: The roles of autophagy and apoptosis in burn wound progression in rats.
abstract: Understanding the role of cell death in burn wound progression is crucial for giving appropriate diagnoses and designing therapy regimens for burn patients. A well-described and reliable "comb burns model" was employed to evaluate the roles of autophagy and apoptosis in burn wound progression at 2 h, 6 h, 12 h, 24 h, and 48 h post-burn in a rat model. Immunohistochemistry (IHC) results showed that autophagy was detectable in hair follicle epithelium at 2 h post-burn, peaked at 12 h post-burn, then declined. Conversely, apoptosis was mainly located in the stratum epidermis and took place at low levels until 6 h post-burn, at which point it slowly increased. Bcl-2 and Bax, which are regulators of both processes, showed protein expression level patterns that were consistent with the IHC results. This study of autophagy in burn wound tissue progression represents a conceptual expansion of cell death in burn wounds. Based on these results, we suggest that different treatments should be performed on a specific post-burn time course depending on the most prevalent type of cell death occurring at that time.
authors.affiliation: Department of Plastic and Burn Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China.
authors.forename: Jia-Qi
authors.initials: JQ
authors.lastname: Tan
authors.forename: Hong-Hui
authors.initials: HH
authors.lastname: Zhang
authors.forename: Zhan-Jun
authors.initials: ZJ
authors.lastname: Lei
authors.forename: Pan
authors.initials: P
authors.lastname: Ren
authors.forename: Chen
authors.initials: C
authors.lastname: Deng
authors.forename: Xue-Yong
authors.initials: XY
authors.lastname: Li
authors.forename: Shao-Zong
authors.initials: SZ
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism; D001343:Autophagy / Q000502:physiology*; D000071186:Beclin-1; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D002056:Burns / Q000378:metabolism / Q000473:pathology*; D004195:Disease Models, Animal; D018450:Disease Progression; D007150:Immunohistochemistry; D008297:Male; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D051017:Apoptosis Regulatory Proteins; D000071186:Beclin-1; C512829:Becn1 protein, rat; D015415:Biomarkers; D019253:Proto-Oncogene Proteins c-bcl-2
keywords: Autophagy; Burns; Cell death; Zone of stasis