title: Analgesic effects of glycoproteins from Panax ginseng root in mice.
abstract: BACKGROUND
  The root of Panax ginseng C.A. Mey has various beneficial pharmacological effects. The present study aimed to evaluate the analgesic activities of glycoproteins from the root of Panax ginseng C.A. Mey in mice.
  
  
  METHODS
  Glycoproteins were isolated and purified from the root of Panax ginseng C.A. Mey. Physicochemical properties and molecular mass were determined by chemical assay and HPLC. Acetic acid-induced writhing and hot-plate tests were employed to study the analgesic effect of glycoproteins and compared with that of aspirin or morphine. The locomotor activity was tested in mice by using actophometer.
  
  
  RESULTS
  Four glycoproteins were obtained. The glycoproteins which protein content was the highest (73.04%) displayed dose-dependent analgesic effect. In writhing test, the glycoproteins significantly inhibited writhes (P<0.001) at the dose of 20 mg/kg by intraperitoneal injection. In hot-plate test, only at the dose of 20 mg/kg prolong the hot-plate latency (P<0.05, at 30 min). In the locomotor activity test, the glycoproteins were significant decrease of motility counts at the dose of 20 and 40 mg/kg.
  
  
  CONCLUSIONS
  These findings collectively indicate that the glycoproteins from the root of Panax ginseng C.A. Mey exhibited significant analgesic activities and the proteins were the active site, providing evidence for its pharmacal use.
authors.affiliation: Jilin Academy of Chinese Medicine and Material Medica Science, Changchun 130012, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.forename: Yinghong
authors.initials: Y
authors.lastname: Chen
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
authors.forename: Haoming
authors.initials: H
authors.lastname: Luo
authors.forename: Ruizhi
authors.initials: R
authors.lastname: Jiang
mesh_terms: D019342:Acetic Acid; D000700:Analgesics / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D005260:Female; D006023:Glycoproteins / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use*; D006358:Hot Temperature; D008297:Male; D051379:Mice; D009020:Morphine / Q000494:pharmacology; D009043:Motor Activity / Q000187:drug effects; D010146:Pain / Q000139:chemically induced / Q000188:drug therapy* / Q000503:physiopathology; D005894:Panax; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots
chemical_list: D000700:Analgesics; D006023:Glycoproteins; D010936:Plant Extracts; D009020:Morphine; D019342:Acetic Acid
keywords: Acetic acid-induced writhing test; Analgesic activity; Glycoprotein; Hot-plate test; Locomotor activity; Root of Panax ginseng C.A. Mey