title: [Analysis on Mycoplasma pirum infection in male HIV/AIDS patients and related 16S rRNA genes in Jiangsu province].
abstract: OBJECTIVE
  To investigate the prevalence of Mycoplasma pirum (Mpi) in male HIV infected patients, and to identify the 16S rRNA gene of Mpi.
  
  
  METHODS
  The first void urine of male HIV/AIDS patients in Jiangsu province was collected for Mpi detection. Purified 16S rRNA gene PCR production was sequenced for analysis on its identification, homogeneity and phylogenetic tree. P1 protein sequence of Mpi was analyzed by Vector NTI Advance 11.0 to calculate the coded amino acid sequence. Homogeneity analysis was conducted between the theoretical amino acid sequence of Mpi and other Mycoplasmas.
  
  
  RESULTS
  The prevalence of Mpi in male HIV/AIDS patients was 21.5% while the Mpi prevalence rates in different age groups were significantly different (χ² Mpi = 124.63, P < 0.01). The homogeneity of 18 strains of Mpi was higher than 90%.
  
  
  CONCLUSIONS
  The Mpi prevalence seemed much higher than the results from previous detection on HIV/AIDS patients, suggesting that more attention should be paid on AIDS treatment. More bioinformatic research on gene/nucleotide sequence analysis and forecast should be carried out to identify the molecular characteristics of Mpi.
authors.affiliation: Department of Epidemiology & Biostatistics, School of Public Health, Southeast University, Nanjing 210009.
authors.forename: Jian-ru
authors.initials: JR
authors.lastname: Wu
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhu
authors.forename: Yan-xin
authors.initials: YX
authors.lastname: Xie
authors.forename: Liang-jia
authors.initials: LJ
authors.lastname: Zhou
authors.forename: Jin-shui
authors.initials: JS
authors.lastname: Xu
authors.forename: Xi-ping
authors.initials: XP
authors.lastname: Huan
authors.forename: Bei
authors.initials: B
authors.lastname: Wang
mesh_terms: D000163:Acquired Immunodeficiency Syndrome / Q000453:epidemiology / Q000382:microbiology*; D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D020459:Genes, rRNA; D015658:HIV Infections / Q000453:epidemiology / Q000382:microbiology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009174:Mycoplasma / Q000145:classification / Q000235:genetics*; D009175:Mycoplasma Infections / Q000453:epidemiology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D055815:Young Adult
chemical_list: D012336:RNA, Ribosomal, 16S