title: Genome-wide comparative analysis revealed significant transcriptome changes in mice after Toxoplasma gondii infection.
abstract: BACKGROUND
  Toxoplasma gondii is an intracellular parasite that can modulate host responses and presumably host behavior. Host responses as well as pathogenesis vary depending on the parasite strains that are responsible for infection. In immune competent individuals, T. gondii preferentially infects tissues of the central nervous systems (CNS), which might be an additional factor in certain psychiatric disorders. While in immune-compromised individuals and pregnant women, the parasite can cause life-threatening infections. With the availability of the genome-wide investigation platform, the global responses in gene expression of the host after T. gondii infection can be systematically investigated.
  
  
  METHODS
  Total RNA of brain tissues and peripheral lymphocytes of BALB/C mice infected with RH and ME 49 strain T. gondii as well as that of healthy mice were purified and converted to cRNA with incorporated Cy5-CTP (experimental samples), or Cy3-CTP (control samples). The labeled cRNA probes were hybridized to the Whole Mouse Genome Microarray. The impact of parasite infection on gene expression in both brain tissues and peripheral lymphocytes were analyzed. Differentially expressed genes were revalidated with real-time quantitative reverse transcriptase-polymerase chain reaction (Q-PCR).
  
  
  RESULTS
  Data indicated that the genes associated with immunity were up-regulated after infection by the two parasite strains, but significant up-regulation was observed in both brain tissues and peripheral lymphocytes of mice infected with ME49 strain compared to that infected by RH strain. The pathways related to pathogenesis of the nervous system were more significantly up-regulated in mice infected with RH strain.
  
  
  CONCLUSIONS
  Genetically distinct T. gondii strains showed clear differences in modulation of host pathophysiological and immunological responses in both brain tissue and peripheral lymphocytes. It was likely that some of the host responses to T. gondii infection were universal, but the immune response and CNS reaction were in a strain-specific manner.
authors.affiliation: Key Laboratory of Zoonosis, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Xi An Da Lu 5333, Changchun 130062, China. jiangning@jlu.edu.cn
authors.forename: Boyin
authors.initials: B
authors.lastname: Jia
authors.forename: Huijun
authors.initials: H
authors.lastname: Lu
authors.forename: Quan
authors.initials: Q
authors.lastname: Liu
authors.forename: Jigang
authors.initials: J
authors.lastname: Yin
authors.forename: Ning
authors.initials: N
authors.lastname: Jiang
authors.forename: Qijun
authors.initials: Q
authors.lastname: Chen
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology; D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D004195:Disease Models, Animal; D005260:Female; D054884:Host-Pathogen Interactions; D008214:Lymphocytes / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D046228:Microarray Analysis; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D060888:Real-Time Polymerase Chain Reaction; D014122:Toxoplasma / Q000254:growth & development* / Q000276:immunology / Q000472:pathogenicity; D014124:Toxoplasmosis, Animal / Q000276:immunology* / Q000469:parasitology / Q000473:pathology*; D059467:Transcriptome
chemical_list: D018076:DNA, Complementary; D012313:RNA