title: Fluorescence amplified detection of proteases by the catalytic activation of a semisynthetic sensor.
abstract: A general approach to mimic the sensing scheme of allosteric enzymes is developed. Through the covalent labeling of a sulfonamide inhibitor to the enzyme HCAII via SNAP-tag protein, the enzyme is rendered inactive. Catalytic activation is triggered only when a protease is present to cleave the recognized peptide sequence.
authors.affiliation: Department of Chemistry, National Tsing Hua University, 101 Sec. 2, Kuang Fu Road, Hsinchu 30013, Taiwan, ROC.
authors.forename: Po-Ming
authors.initials: PM
authors.lastname: Shih
authors.forename: Tao-Kai
authors.initials: TK
authors.lastname: Liu
authors.forename: Kui-Thong
authors.initials: KT
authors.lastname: Tan
mesh_terms: D024402:Carbonic Anhydrase II / Q000737:chemistry*; D002384:Catalysis; D005453:Fluorescence; D009842:Oligopeptides / Q000737:chemistry; D010447:Peptide Hydrolases / Q000737:chemistry*; D011993:Recombinant Fusion Proteins / Q000737:chemistry*; D013449:Sulfonamides / Q000037:antagonists & inhibitors
chemical_list: D009842:Oligopeptides; D011993:Recombinant Fusion Proteins; D013449:Sulfonamides; D010447:Peptide Hydrolases; D024402:Carbonic Anhydrase II