title: Genome sequence analysis of the Vibrio parahaemolyticus lytic bacteriophage VPMS1.
abstract: VPMS1 is a Vibrio parahaemolyticus lytic phage isolated from a marine clam. The 42.3-kb genome was predicted to encode 53 proteins. Comparison of the VPMS1 DNA genome with known phage genomes revealed no similarity; hence, it represents a new VP phage, organized into three differently oriented modules. The module for packaging covers 12 % of the genome, the module for structure covers 31 %, and the module for replication and regulation covers 48 %. The G + C content was 44.67 %. The coding region corresponds to 91 % of the genome, and 9 % apparently does not encode any protein. Thirty genes, constituting 57 % of the genome, had significant similarity to some reported proteins in the protein database; 23 genes, constituting 43 % of the genome, showed no significant homology to any reported protein, and these could be new proteins whose hypothetical functions can be deduced from their position in the genome.
authors.affiliation: Centro de Investigaciones Biológicas del Noroeste (CIBNOR), Instituto Politécnico Nacional, 195, Col. Playa Palo de Santa Rita Sur, 23096, La Paz, B.C.S, Mexico, mramirez04@cibnor.mx.
authors.forename: Martín
authors.initials: M
authors.lastname: Ramírez-Orozco
authors.forename: Vania
authors.initials: V
authors.lastname: Serrano-Pinto
authors.forename: Norma
authors.initials: N
authors.lastname: Ochoa-Álvarez
authors.forename: Roman
authors.initials: R
authors.lastname: Makarov
authors.forename: Sergio F
authors.initials: SF
authors.lastname: Martínez-Díaz
mesh_terms: D000818:Animals; D001435:Bacteriophages / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology* / Q000648:ultrastructure; D001482:Base Composition; D049872:Bivalvia / Q000821:virology; D016679:Genome, Viral; D008242:Lysogeny; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D014736:Vibrio parahaemolyticus / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins