title: Nerve growth factor receptor TrkA, a new receptor in insulin signaling pathway in PC12 cells.
abstract: TrkA is a cell surface transmembrane receptor tyrosine kinase for nerve growth factor (NGF). TrkA has an NPXY motif and kinase regulatory loop similar to insulin receptor (INSR) suggesting that NGF→TrkA signaling might overlap with insulin→INSR signaling. During insulin or NGF stimulation TrkA, insulin receptor substrate-1 (IRS-1), INSR (and presumably other proteins) forms a complex in PC12 cells. In PC12 cells, tyrosine phosphorylation of INSR and IRS-1 is dependent upon the functional TrkA kinase domain. Moreover, expression of TrkA kinase-inactive mutant blocked the activation of Akt and Erk5 in response to insulin or NGF. Based on these data, we propose that TrkA participates in insulin signaling pathway in PC12 cells.
authors.affiliation: Department of Nutrition, Dietetics, and Hospitality Management, Auburn University, Auburn, Alabama 36849,USA.
authors.forename: Thangiah
authors.initials: T
authors.lastname: Geetha
authors.forename: Shraddha D
authors.initials: SD
authors.lastname: Rege
authors.forename: Salome E
authors.initials: SE
authors.lastname: Mathews
authors.forename: Susan O
authors.initials: SO
authors.lastname: Meakin
authors.forename: Morris F
authors.initials: MF
authors.lastname: White
authors.forename: Jeganathan Ramesh
authors.initials: JR
authors.lastname: Babu
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D004789:Enzyme Activation / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000378:metabolism* / Q000494:pharmacology; D055504:Insulin Receptor Substrate Proteins / Q000378:metabolism; D048054:Mitogen-Activated Protein Kinase 7 / Q000378:metabolism; D008969:Molecular Sequence Data; D020932:Nerve Growth Factor / Q000378:metabolism* / Q000494:pharmacology; D016716:PC12 Cells; D010766:Phosphorylation / Q000187:drug effects; D019000:Phosphotyrosine / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D051381:Rats; D011972:Receptor, Insulin / Q000737:chemistry / Q000378:metabolism; D020917:Receptor, trkA / Q000737:chemistry / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D007328:Insulin; D055504:Insulin Receptor Substrate Proteins; D019000:Phosphotyrosine; D020932:Nerve Growth Factor; D011972:Receptor, Insulin; D020917:Receptor, trkA; D051057:Proto-Oncogene Proteins c-akt; D048054:Mitogen-Activated Protein Kinase 7; D005947:Glucose
keywords: Akt; IRS-1; Insulin; Insulin Receptor; NGF; Neurotrophins; Protein-Protein Interactions; Signal Transduction; TrkA