title: Effects of tert-butyl hydroperoxide on Ca(2+) ATPase activity in isolated rat hepatocytes and its reversal by antioxidants.
abstract: Calcium ions play an importantrole in various physiological processes such as nerve impulse transmission, muscle contraction, hormone action, blood clotting. They ions act as an intracellular second messenger, relaying information within cells to regulate their activity. To understand the mechanism of hepatotoxicity of t-BHP, studies were carried out using freshly isolated rat hepatocytes. The effect of t-BHP on Ca(2+) accumulation and Ca(2+) uptake by rat hepatocytes was monitored using 45Ca(2+). It caused decrease in 15% accumulation of 45Ca(2+) in comparison to the control group. t-BHP also significantly decreased the Ca(2+) ATPase activity in isolated hepatocytes .This decrease in Ca(2+) ATPase activity by t-BHP was reversed 40% by naturally occurring antioxidant glutathione (GSH) and 20% by the synthetic antioxidant butylated hydroxy toluene (BHT). These results indicate that the hepatotoxic action of t-BHP involves oxidative stress as evident by the protection accorded by various antioxidants employed in the study as well as impairment of intracellular calcium homeostasis which can lead to liver cell injury.
authors.affiliation: Department of Biochemistry, Dr. R. M. L. Avadh University, Faizabad-224001, India. sangram_rml@yahoo.co.in
authors.forename: Sangram
authors.initials: S
authors.lastname: Singh
authors.forename: Richa
authors.initials: R
authors.lastname: Agarwal
authors.forename: Farrukh
authors.initials: F
authors.lastname: Jamal
authors.forename: Sudhir
authors.initials: S
authors.lastname: Mehrotra
authors.forename: Rakesh
authors.initials: R
authors.lastname: Singh
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002084:Butylated Hydroxytoluene / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D000252:Calcium-Transporting ATPases / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects* / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D020122:tert-Butylhydroperoxide / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D002084:Butylated Hydroxytoluene; D020122:tert-Butylhydroperoxide; D000252:Calcium-Transporting ATPases; D005978:Glutathione; D002118:Calcium