title: Mandelate racemase and mandelate dehydrogenase coexpressed recombinant Escherichia coli in the synthesis of benzoylformate.
abstract: Benzoylformate is used widely as an organic synthetic intermediate. Mandelate racemase and mandelate dehydrogenase are two enzymes used in producing benzoylformate from racemic mandelate in microbial metabolism. In the present work, the genes encoding mandelate racemase and mandelate dehydrogenase from Pseudomonas aeruginosa strain NUST were cloned and expressed in Escherichia coli to convert racemic mandelate to benzoylformate. The use of whole resting cells of recombinant E. coli allowed the conversion of 65.7 mM solution of racemic mandelate to benzoylformate at a yield of 98.9% in 45 h. The process is a promising alternative for the production of benzoylformate.
authors.affiliation: Department of Bioengineering, Nanjing University of Science and Technology, Nanjing, PR China.
authors.forename: Dali
authors.initials: D
authors.lastname: Li
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zeng
authors.forename: Junfang
authors.initials: J
authors.lastname: Yang
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Jiang
authors.forename: Jing
authors.initials: J
authors.lastname: Feng
authors.forename: Chengli
authors.initials: C
authors.lastname: Yang
mesh_terms: D000595:Amino Acid Sequence; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005285:Fermentation; D006038:Glyoxylates / Q000737:chemistry / Q000378:metabolism*; D008333:Mandelic Acids / Q000737:chemistry / Q000378:metabolism*; D010088:Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D010648:Phenylacetates / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000235:genetics / Q000378:metabolism; D019998:Racemases and Epimerases / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism*
chemical_list: D006038:Glyoxylates; D008333:Mandelic Acids; D010648:Phenylacetates; D011994:Recombinant Proteins; C012482:phenylglyoxylic acid; D010088:Oxidoreductases; D019998:Racemases and Epimerases; C021540:mandelate racemase