title: Persistence of apoptosis and inflammatory responses in the heart and bone marrow of mice following whole-body exposure to ²⁸Silicon (²⁸Si) ions.
abstract: It has been well established that the bone marrow (BM) is a radiosensitive tissue, but the radiosensitivity of the heart is poorly understood. In this study, we investigated the comparative effects of ²⁸Silicon (²⁸Si) ions (one type of heavy ion found in space) on tissue from the heart and the BM of exposed mice. We gave adult male CBA/CaJ mice a whole-body exposure to a total dose of 0, 0.1, 0.25, or 0.5 Gy of 300 MeV/nucleon (n) ²⁸Si ions, using a fractionated schedule (two exposures, 15 days apart that totaled each selected dose). The heart and BM were collected from 5 mice per treatment group at various times up to 6 months post-irradiation. In each mouse, we obtained tissue lysates from the heart and from the total population of BM cells for measuring the levels of cleaved poly (ADP-ribose) polymerase (cleaved PARP, a marker of apoptotic cell death) and the levels of activated nuclear factor-kappa B (NF-κB) and selected NF-κB-regulated cytokines known to be involved in inflammatory responses. Our data showed that, up to 6 months post-irradiation, the levels of apoptotic cell death and inflammatory responses in tissues from the heart and BM collected from exposed mice were statistically higher than those in sham controls. Hence, these findings are suggestive of chronic apoptotic cell death and inflammation in both tissues after exposure to ²⁸Si ions. In summary, our data are indicative of a possible association between exposure to ²⁸Si ions during space flight and long-term health risk.
authors.affiliation: Pathology Department, Stony Brook University, Stony Brook, NY 11794-8691, USA.
authors.forename: Montree
authors.initials: M
authors.lastname: Tungjai
authors.forename: Elbert B
authors.initials: EB
authors.lastname: Whorton
authors.forename: Kanokporn Noy
authors.initials: KN
authors.lastname: Rithidech
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D001853:Bone Marrow / Q000378:metabolism / Q000528:radiation effects*; D016207:Cytokines / Q000378:metabolism; D006321:Heart / Q000528:radiation effects*; D007249:Inflammation; D008297:Male; D051379:Mice; D008808:Mice, Inbred CBA; D009206:Myocardium / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D011868:Radioisotopes / Q000009:adverse effects*; D012825:Silicon / Q000009:adverse effects*; D014916:Whole-Body Irradiation
chemical_list: D016207:Cytokines; D016328:NF-kappa B; D011868:Radioisotopes; C499321:Parp1 protein, mouse; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases; D012825:Silicon