title: Nuclear proteome profile of C57BL/6J mouse liver.
abstract: The liver proteome can serve as a reference to better understand both disease mechanisms and possible therapeutics, since the liver is an important organ in the body that performs a large number of tasks. Here we identify the organelle proteome of C57BL/6J mouse liver nuclei as a promising strategy to enrich low abundance proteins, in the sense that analysis of whole liver cells is rather complex for current techniques and may not be suitable for proteins with low abundance. Evaluation of nucleus integrity and purity was performed to demonstrate the effectiveness of the optimized isolation procedure. The extracted nuclear proteins were identified by 2-DE MS analyses, and a total of 748 proteins were identified. Bioinformatic analyses were performed to demonstrate the physicochemical properties, cellular locations and functions of the proteins.
authors.affiliation: School of Life Sciences and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Caiyun
authors.initials: C
authors.lastname: Fang
authors.forename: Huimin
authors.initials: H
authors.lastname: Bao
authors.forename: Huizhi
authors.initials: H
authors.lastname: Fan
authors.forename: Huali
authors.initials: H
authors.lastname: Shen
authors.forename: Pengyuan
authors.initials: P
authors.lastname: Yang
mesh_terms: D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D008099:Liver / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D009687:Nuclear Proteins / Q000032:analysis* / Q000302:isolation & purification; D060066:Protein Interaction Maps; D020543:Proteome / Q000032:analysis* / Q000302:isolation & purification; D040901:Proteomics / Q000379:methods*
chemical_list: D009687:Nuclear Proteins; D020543:Proteome