title: Isolation of fucoxanthin and highly unsaturated monogalactosyldiacylglycerol from brown alga Fucus evanescens C Agardh and in vitro investigation of their antitumor activity.
abstract: Fucoxanthin (FX) and highly unsaturated monogalactosyldiacylglycerol (MGDG) were isolated from the ethanol extract of brown alga Fucus evanescens. Their structures were identified by nuclear magnetic resonance, complemented by electrospray ionization mass spectrometry (ESIMS). MGDG was identified as 1-O-(5Z,8Z,11Z,14Z,17Z-eicosapentanoyl)-2-O-(6Z,9Z,12Z,15Z-octadecatetraenoyl)-3-O-β-D-galactopiranosyl-sn-glycerol. Antitumor activity of these compounds was tested on human melanoma (SK-MEL-28) cells. MGDG and FX inhibited the growth of human melanoma cells in a dose-dependent manner. IC50 values for growth inhibition were 104 and 114 μM, correspondently.
authors.affiliation: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Prospect 100-letya Vladivostoka, 159, Vladivostok, 690022, Russia. tatyanaimbs@mail.ru
authors.forename: Tatiana I
authors.initials: TI
authors.lastname: Imbs
authors.forename: Svetlana P
authors.initials: SP
authors.lastname: Ermakova
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Fedoreyev
authors.forename: Stanislav D
authors.initials: SD
authors.lastname: Anastyuk
authors.forename: Tatiana N
authors.initials: TN
authors.lastname: Zvyagintseva
mesh_terms: D000970:Antineoplastic Agents / Q000032:analysis / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002855:Chromatography, Thin Layer; D045424:Complex Mixtures / Q000032:analysis; D004305:Dose-Response Relationship, Drug; D000431:Ethanol; D005227:Fatty Acids / Q000032:analysis; D044645:Fucus / Q000737:chemistry*; D038983:Galactolipids / Q000032:analysis / Q000302:isolation & purification*; D006801:Humans; D020128:Inhibitory Concentration 50; D013058:Mass Spectrometry; D019906:Nuclear Magnetic Resonance, Biomolecular; D024341:Xanthophylls / Q000032:analysis / Q000302:isolation & purification*
chemical_list: D000970:Antineoplastic Agents; D045424:Complex Mixtures; D005227:Fatty Acids; D038983:Galactolipids; D024341:Xanthophylls; C009909:monogalactosyldiacylglycerol; C025164:fucoxanthin; D000431:Ethanol