title: Identification of new HLA-A*0201-restricted cytotoxic T lymphocyte epitopes from neuritin.
abstract: Identification of cytotoxic T lymphocyte (CTL) epitopes from additional tumor antigens is essential for the development of specific immunotherapy of malignant tumors. Neuritin, a recently discovered antigen overexpressed in astrocytoma, is considered to be a promising target for biological therapy. In the present study, we predicted and identified HLA-A2-restricted CTL epitopes from neuritin by using the following four-step procedure: (1) computer-based epitope prediction from the amino acid sequence of neuritin; (2) peptide-binding assay to determine the affinity of the predicted peptide with HLA-A2.1 molecule; (3) stimulation of primary T cell response against the predicted peptides in vitro; and (4) testing of the induced CTLs toward target cells expressing neuritin and HLA-A2.1. The results demonstrated that effectors induced by peptides of neuritin containing residues 13-21, 121-129 and 4-12 could specifically-secrete interferon-γ and lyse target cells. Our results indicate that these peptides are new HLA-A2.1-restricted CTL epitopes, and may serve as valuable tools for astrocytoma immunotherapy.
authors.affiliation: Department of Neurology, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Yang
authors.forename: Tianzhi
authors.initials: T
authors.lastname: Zhao
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.forename: Zili
authors.initials: Z
authors.lastname: Gong
authors.forename: Saiyu
authors.initials: S
authors.lastname: Cheng
authors.forename: Qingwu
authors.initials: Q
authors.lastname: Yang
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000378:metabolism; D001666:Binding Sites, Antibody / Q000187:drug effects / Q000235:genetics; D045744:Cell Line, Tumor; D003713:Dendritic Cells / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D000939:Epitopes / Q000378:metabolism*; D058851:GPI-Linked Proteins / Q000378:metabolism; D015789:HLA-A2 Antigen / Q000378:metabolism*; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D027701:Organic Cation Transport Proteins / Q000235:genetics / Q000378:metabolism; D010455:Peptides / Q000494:pharmacology; D013602:T-Lymphocytes, Cytotoxic / Q000378:metabolism*
chemical_list: D015703:Antigens, CD; D000939:Epitopes; D058851:GPI-Linked Proteins; C435939:HLA-A*02:01 antigen; D015789:HLA-A2 Antigen; D015395:Histocompatibility Antigens Class I; D009419:Nerve Tissue Proteins; C484377:Nrn1 protein, mouse; D027701:Organic Cation Transport Proteins; D010455:Peptides; C510732:SLC44A1 protein, human; D007371:Interferon-gamma