title: Picornavirus uncoating intermediate captured in atomic detail.
abstract: It remains largely mysterious how the genomes of non-enveloped eukaryotic viruses are transferred across a membrane into the host cell. Picornaviruses are simple models for such viruses, and initiate this uncoating process through particle expansion, which reveals channels through which internal capsid proteins and the viral genome presumably exit the particle, although this has not been clearly seen until now. Here we present the atomic structure of an uncoating intermediate for the major human picornavirus pathogen CAV16, which reveals VP1 partly extruded from the capsid, poised to embed in the host membrane. Together with previous low-resolution results, we are able to propose a detailed hypothesis for the ordered egress of the internal proteins, using two distinct sets of channels through the capsid, and suggest a structural link to the condensed RNA within the particle, which may be involved in triggering RNA release.
authors.affiliation: Division of Structural Biology, University of Oxford, Headington, Oxford, UK.
authors.forename: Jingshan
authors.initials: J
authors.lastname: Ren
authors.forename: Xiangxi
authors.initials: X
authors.lastname: Wang
authors.forename: Zhongyu
authors.initials: Z
authors.lastname: Hu
authors.forename: Qiang
authors.initials: Q
authors.lastname: Gao
authors.forename: Yao
authors.initials: Y
authors.lastname: Sun
authors.forename: Xuemei
authors.initials: X
authors.lastname: Li
authors.forename: Claudine
authors.initials: C
authors.lastname: Porta
authors.forename: Thomas S
authors.initials: TS
authors.lastname: Walter
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Gilbert
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Zhao
authors.forename: Danny
authors.initials: D
authors.lastname: Axford
authors.forename: Mark
authors.initials: M
authors.lastname: Williams
authors.forename: Katherine
authors.initials: K
authors.lastname: McAuley
authors.forename: David J
authors.initials: DJ
authors.lastname: Rowlands
authors.forename: Weidong
authors.initials: W
authors.lastname: Yin
authors.forename: Junzhi
authors.initials: J
authors.lastname: Wang
authors.forename: David I
authors.initials: DI
authors.lastname: Stuart
authors.forename: Zihe
authors.initials: Z
authors.lastname: Rao
authors.forename: Elizabeth E
authors.initials: EE
authors.lastname: Fry
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D018360:Crystallography, X-Ray; D004770:Enterovirus; D006801:Humans; D008958:Models, Molecular; D008968:Molecular Conformation; D010849:Picornaviridae / Q000737:chemistry* / Q000502:physiology*; D014709:Vero Cells; D015678:Viral Structural Proteins / Q000737:chemistry; D014771:Virion / Q000378:metabolism; D053586:Virus Internalization; D057646:Virus Uncoating / Q000502:physiology*
chemical_list: D015678:Viral Structural Proteins