title: Methylation analysis of tumor suppressor genes in endometroid carcinoma of endometrium using MS-MLPA.
abstract: BACKGROUND
  Epigenetic changes are considered to be a frequent event during tumor development. Hypermethylation of promoter CpG islands represents an alternative mechanism for inactivation of tumor suppressor genes, DNA repair genes, cell cycle regulators and transcription factors. The aim of this study was to investigate promoter methylation of specific genes in endometrial cancer by comparison with normal endometrial tissue.
  
  
  METHODS
  We used MS-MLPA (Methylation-specific Multiplex ligation-dependent probe amplification) to compare the methylation status of 59 tissue samples of endometroid type of endometrial carcinoma with 20 control samples of non-neoplastic endometrium.
  
  
  RESULTS
  Using 15% cut-off for methylation, we observed significantly higher methylation in the CDH13 gene in endometrial cancer group. We observed significantly higher methylation in both WT1 and GATA5 genes in IB stage of endometroid carcinoma. We also observed significantly higher methylation in GATA5 gene in the group of poorly differentiated endometroid carcinoma.
  
  
  CONCLUSIONS
  The findings suggest the importance of hypermethylation of CDH13, WT1 and GATA5 genes in endometrial carcinogenesis and could have implications for future diagnostic and therapeutic strategies of endometrial cancer based on epigenetic changes.
authors.affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine in Hradec Kralove, Charles University in Prague and University Hospital Hradec Kralove, Hradec Kralove, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Dvorakova
authors.forename: Marcela
authors.initials: M
authors.lastname: Chmelarova
authors.forename: Jan
authors.initials: J
authors.lastname: Laco
authors.forename: Vladimir
authors.initials: V
authors.lastname: Palicka
authors.forename: Jiri
authors.initials: J
authors.lastname: Spacek
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002277:Carcinoma / Q000235:genetics*; D019175:DNA Methylation; D016889:Endometrial Neoplasms / Q000235:genetics*; D005260:Female; D016147:Genes, Tumor Suppressor; D006801:Humans; D008875:Middle Aged; D060885:Multiplex Polymerase Chain Reaction