title: Lycium barbarum polysaccharides as an adjuvant for recombinant vaccine through enhancement of humoral immunity by activating Tfh cells.
abstract: Lycium barbarum polysaccharides (LBP) have been shown to play a variety of immune-modulatory functions which include activation of T and B cells. Follicular helper T (Tfh) cells are now recognized as a subset of helper T cells which regulate the multiple stages of B cell maturation and function. In our current study, we found that LBP were able to activate CXCR5+PD-1+ Tfh cells and induce IL-21 secretion. At the same time LBP also promoted the formation of germinal centers (GC) and production of GL-7+B220+GC B cells. LBP as an adjuvant could increase generation of rAd5VP1-induced Tfh cells. Our results indicate that LBP might enhance T cell-dependent Ab responses by acting as an adjuvant for the generation of Tfh cells. Future vaccine designs might therefore be targeted to induce strong antigen-specific Tfh responses in order to boost its protective effects.
authors.affiliation: School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, Ningxia, 750004, PR China. Electronic address: chunxiasu@yahoo.com.cn.
authors.forename: Chun-xia
authors.initials: CX
authors.lastname: Su
authors.affiliation: Laboratory Examination College, Ningxia Medical University, Yinchuan, Ningxia, 750004, PR China; Laboratory Examination Research Institute, Ningxia Medical University, Yinchuan, Ningxia, 750004, PR China; Stem Cell Research Institute, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, 750004, PR China.
authors.forename: Xiang-guo
authors.initials: XG
authors.lastname: Duan
authors.affiliation: General Hospital of Ningxia Medical University, Yinchuan, Ningxia, 750004, PR China.
authors.forename: Li-jun
authors.initials: LJ
authors.lastname: Liang
authors.affiliation: School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, Ningxia, 750004, PR China.
authors.lastname: Feng-Wang
authors.affiliation: School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, Ningxia, 750004, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Pharmacy College, Ningxia Medical University, Yinchuan, Ningxia, 750004, PR China.
authors.forename: Xue-Yan
authors.initials: XY
authors.lastname: Fu
authors.affiliation: Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan, Ningxia, 640100, PR China.
authors.forename: Ya-mei
authors.initials: YM
authors.lastname: Yan
authors.affiliation: School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, Ningxia, 750004, PR China.
authors.lastname: Ling-Huang
authors.affiliation: School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, Ningxia, 750004, PR China.
authors.forename: Ning-ping
authors.initials: NP
authors.lastname: Wang
mesh_terms: D000276:Adjuvants, Immunologic / Q000494:pharmacology*; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D056724:Immunity, Humoral / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence; D061026:Programmed Cell Death 1 Receptor / Q000032:analysis / Q000276:immunology; D054380:Receptors, CXCR5 / Q000032:analysis / Q000276:immunology; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D014614:Vaccines, Synthetic / Q000276:immunology*
chemical_list: D000276:Adjuvants, Immunologic; C513179:CXCR5 protein, mouse; D004365:Drugs, Chinese Herbal; C491383:Pdcd1 protein, mouse; D061026:Programmed Cell Death 1 Receptor; D054380:Receptors, CXCR5; D014614:Vaccines, Synthetic; C500223:lycium barbarum polysaccharide
keywords: Adjuvant; Follicular helper T cells; Lycium barbarum polysaccharides