title: Detectability of lupine seeds by ELISA and PCR may be strongly influenced by potential differences between cultivars.
abstract: Accurate methods for allergen detection are needed for the verification of allergen labeling and the avoidance of hidden allergens. But systematic data on the influence of different cultivars of allergenic crop species on their detectability in enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) are lacking. As one example, seeds of 14 different cultivars of lupine (Lupinus albus, Lupinus angustifolius, Lupinus luteus) were investigated for total protein according to a Kjeldahl method, and for their relative quantitative detectability in three commercial lupine-specific ELISA tests and four lupine-specific PCR methods. Total Kjeldahl nitrogen allowed an accurate quantification of total protein. Relative differences in quantitative response between cultivars of 390-5050% and 480-13,600% were observed between ELISA kits and PCR methods, respectively. Hence, quantitative results of selected ELISA and PCR methods may be strongly influenced by the examined lupine cultivar.
authors.affiliation: Division of Allergology, Paul-Ehrlich-Institut, ‡Division of Immunology; Paul-Ehrlich-Institut , Paul-Ehrlich-Strasse 51-59, 63225 Langen, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Röder
authors.forename: Kornelia
authors.initials: K
authors.lastname: Kleiner
authors.forename: Andrea
authors.initials: A
authors.lastname: Sachs
authors.forename: Nicole
authors.initials: N
authors.lastname: Keil
authors.forename: Thomas
authors.initials: T
authors.lastname: Holzhauser
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D031297:Lupinus / Q000737:chemistry* / Q000145:classification / Q000235:genetics; D010936:Plant Extracts / Q000032:analysis*; D016133:Polymerase Chain Reaction / Q000379:methods*; D012639:Seeds / Q000737:chemistry / Q000145:classification / Q000235:genetics
chemical_list: D010936:Plant Extracts