title: The Tox21 robotic platform for the assessment of environmental chemicals--from vision to reality.
abstract: Since its establishment in 2008, the US Tox21 inter-agency collaboration has made great progress in developing and evaluating cellular models for the evaluation of environmental chemicals as a proof of principle. Currently, the program has entered its production phase (Tox21 Phase II) focusing initially on the areas of modulation of nuclear receptors and stress response pathways. During Tox21 Phase II, the set of chemicals to be tested has been expanded to nearly 10,000 (10K) compounds and a fully automated screening platform has been implemented. The Tox21 robotic system combined with informatics efforts is capable of screening and profiling the collection of 10K environmental chemicals in triplicate in a week. In this article, we describe the Tox21 screening process, compound library preparation, data processing, and robotic system validation.
authors.affiliation: NIH Chemical Genomics Center, National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, MD 20850, USA.
authors.forename: Matias S
authors.initials: MS
authors.lastname: Attene-Ramos
authors.forename: Nicole
authors.initials: N
authors.lastname: Miller
authors.forename: Ruili
authors.initials: R
authors.lastname: Huang
authors.forename: Sam
authors.initials: S
authors.lastname: Michael
authors.forename: Misha
authors.initials: M
authors.lastname: Itkin
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Kavlock
authors.forename: Christopher P
authors.initials: CP
authors.lastname: Austin
authors.forename: Paul
authors.initials: P
authors.lastname: Shinn
authors.forename: Anton
authors.initials: A
authors.lastname: Simeonov
authors.forename: Raymond R
authors.initials: RR
authors.lastname: Tice
authors.forename: Menghang
authors.initials: M
authors.lastname: Xia
mesh_terms: D000818:Animals; D004784:Environmental Monitoring / Q000295:instrumentation / Q000379:methods*; D004785:Environmental Pollutants / Q000737:chemistry*; D006801:Humans; D012371:Robotics / Q000295:instrumentation / Q000379:methods*; D014481:United States; D014484:United States Environmental Protection Agency / Q000639:trends
chemical_list: D004785:Environmental Pollutants