title: Cloning and polymorphisms of yak lactate dehydrogenase B gene.
abstract: The main objective of this work was to study the unique polymorphisms of the lactate dehydrogenase-1 (LDH1) gene in yak (Bos grunniens). Native polyacrylamide gel electrophoresis revealed three phenotypes of LDH1 (a tetramer of H subunit) in yak heart and longissimus muscle extracts. The corresponding gene, ldhb, encoding H subunits of three LDH1 phenotypes was obtained by RT-PCR. A total of six nucleotide differences were detected in yak ldhb compared with that of cattle, of which five mutations cause amino acid substitutions. Sequence analysis shows that the G896A and C689A, mutations of ldhb gene, result in alterations of differently charged amino acids, and create the three phenotypes (F, M, and S) of yak LDH1. Molecular modeling of the H subunit of LDH indicates that the substituted amino acids are not located within NAD+ or substrate binding sites. PCR-RFLP examination of G896A mutation demonstrated that most LDH1-F samples are actually heterozygote at this site. These results help to elucidate the molecular basis and genetic characteristic of the three unique LDH1 phenotypes in yak.
authors.affiliation: College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China. yucaizheng65@hotmail.com.
authors.forename: Guosheng
authors.initials: G
authors.lastname: Wang
authors.forename: Xingbo
authors.initials: X
authors.lastname: Zhao
authors.forename: Juming
authors.initials: J
authors.lastname: Zhong
authors.forename: Meng
authors.initials: M
authors.lastname: Cao
authors.forename: Qinghua
authors.initials: Q
authors.lastname: He
authors.forename: Zhengxin
authors.initials: Z
authors.lastname: Liu
authors.forename: Yaqiu
authors.initials: Y
authors.lastname: Lin
authors.forename: Yaou
authors.initials: Y
authors.lastname: Xu
authors.forename: Yucai
authors.initials: Y
authors.lastname: Zheng
mesh_terms: D000483:Alleles; D000818:Animals; D001947:Breeding; D002417:Cattle / Q000235:genetics*; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D007526:Isoelectric Point; D007527:Isoenzymes / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008958:Models, Molecular; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D021122:Protein Subunits / Q000235:genetics; D017422:Sequence Analysis, DNA; D055672:Static Electricity
chemical_list: D018076:DNA, Complementary; D007527:Isoenzymes; D021122:Protein Subunits; D007770:L-Lactate Dehydrogenase; C409667:lactate dehydrogenase 1