title: Comparative toxicokinetics of organic micropollutants in freshwater crustaceans.
abstract: Exposure and depuration experiments for Gammarus pulex and Daphnia magna were conducted to quantitatively analyze biotransformation products (BTPs) of organic micropollutants (tramadol, irgarol, and terbutryn). Quantification for BTPs without available standards was performed using an estimation method based on physicochemical properties. Time-series of internal concentrations of micropollutants and BTPs were used to estimate the toxicokinetic rates describing uptake, elimination, and biotransformation processes. Bioaccumulation factors (BAF) for the parents and retention potential factors (RPF), representing the ratio of the internal amount of BTPs to the parent at steady state, were calculated. Nonlinear correlation of excretion rates with hydrophobicity indicates that BTPs with lower hydrophobicity are not always excreted faster than the parent compound. For irgarol, G.pulex showed comparable elimination, but greater uptake and BAF/RPF values than D.magna. Further, G. pulex had a whole set of secondary transformations that D. magna lacked. Tramadol was transformed more and faster than irgarol and there were large differences in toxicokinetic rates for the structurally similar compounds irgarol and terbutryn. Thus, predictability of toxicokinetics across species and compounds needs to consider biotransformation and may be more challenging than previously thought because we found large differences in closely related species and similar chemical structures.
authors.affiliation: Eawag, Swiss Federal Institute of Aquatic Science and Technology , 8600 Dübendorf, Switzerland. junho.jeon@eawag.ch
authors.forename: Junho
authors.initials: J
authors.lastname: Jeon
authors.forename: Denise
authors.initials: D
authors.lastname: Kurth
authors.forename: Roman
authors.initials: R
authors.lastname: Ashauer
authors.forename: Juliane
authors.initials: J
authors.lastname: Hollender
mesh_terms: D000818:Animals; D001711:Biotransformation; D002138:Calibration; D002853:Chromatography, Liquid; D003445:Crustacea / Q000187:drug effects* / Q000378:metabolism; D005618:Fresh Water; D021241:Spectrometry, Mass, Electrospray Ionization; D014227:Triazines / Q000493:pharmacokinetics / Q000633:toxicity; D014874:Water Pollutants, Chemical / Q000493:pharmacokinetics* / Q000633:toxicity*
chemical_list: D014227:Triazines; D014874:Water Pollutants, Chemical; C103341:irgarol 1051; C010347:terbutryne