title: Time exposure period of bovine oocytes to sperm in relation to embryo development rate and quality.
abstract: The objective of the study was to determine the effect of different bovine gamete coincubation times on fertilization and embryo development performance. In vitro matured COCs were co-incubated with sperm at a concentration of 1.5 × 10(6) spermatozoa/ml in TALP medium for 3 hours (T 3, n = 362), 6 hours (T 6, n = 358), or 18 hours (T 18, n = 350). At the end of the coincubation period COCs from times 3 and 6 groups were post-incubated in a new well of fertilization medium without sperm for additional 15 and 12 h, respectively. Cumulus Oocyte Complexes from the T 18 were co-incubated with the sperm suspension for 18 hours. Presumptive zygotes were cultured for 9 days and embryo development was evaluated on days 2, 8, and 9. Thirty blastocysts from each group were stained and total number of nuclei was recorded. The mean (± SEM) percentages of zygotes to develop into ≥2 cell stage were 71.9 ± 5.0; 72.5 ± 5.3 and 81.2 ± 6.1 % for T 3, 6, and 18, respectively, on day 2 and they did not differ (P = .3) among groups. The mean percentage of blastocysts developed on day 8 (25.6 ± 2.8; 24.2 ± 3.3; 28.4 ± 4.2 % for T 3, 6, and 18, resp.) did not differ (P = .4) among groups. The total number of embryonic nuclei was greater (P < .05) for the blastocysts produced from the shortest co-incubation time (T 3).
authors.affiliation: Laboratory of Animal Reproduction, Veterinary School, Faculty of Natural Resources, Catholic University of Temuco, P.O. Box 4780000, Temuco, Chile.
authors.forename: Marco
authors.initials: M
authors.lastname: Berland
authors.forename: Mario
authors.initials: M
authors.lastname: Frei
authors.forename: Oscar
authors.initials: O
authors.lastname: Peralta
authors.forename: Marcelo
authors.initials: M
authors.lastname: Ratto