title: MicroRNA 181 suppresses porcine reproductive and respiratory syndrome virus (PRRSV) infection by targeting PRRSV receptor CD163.
abstract: We previously showed that microRNA 181 (miR-181) can inhibit PRRSV replication by directly targeting its genomic RNA. Here, we report that miR-181 can downregulate the PRRSV receptor CD163 in blood monocytes and porcine alveolar macrophages (PAMs) through targeting the 3' untranslated region (UTR) of CD163 mRNA. Downregulation of CD163 leads to the inhibition of PRRSV entry into PAMs and subsequently suppresses PRRSV infection. Our findings indicate that delivery of miR-181 can be used as antiviral therapy against PRRSV infection.
authors.affiliation: State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Gao
authors.forename: Xue-kun
authors.initials: XK
authors.lastname: Guo
authors.forename: Lianghai
authors.initials: L
authors.lastname: Wang
authors.forename: Qiong
authors.initials: Q
authors.lastname: Zhang
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.forename: Xin-xin
authors.initials: XX
authors.lastname: Chen
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Wang
authors.forename: Wen-hai
authors.initials: WH
authors.lastname: Feng
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics; D015214:Antigens, Differentiation, Myelomonocytic / Q000235:genetics; D016676:Macrophages, Alveolar / Q000821:virology; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009000:Monocytes / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000254:growth & development* / Q000502:physiology; D011956:Receptors, Cell Surface / Q000037:antagonists & inhibitors* / Q000235:genetics; D011991:Receptors, Virus / Q000037:antagonists & inhibitors* / Q000235:genetics; D013552:Swine; D053586:Virus Internalization; D000098902:CD163 Antigen
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D000098902:CD163 Antigen; D035683:MicroRNAs; D011956:Receptors, Cell Surface; D011991:Receptors, Virus