title: High quality genome-wide genotyping from archived dried blood spots without DNA amplification.
abstract: Spots of blood are routinely collected from newborn babies onto filter paper called Guthrie cards and used to screen for metabolic and genetic disorders. The archived dried blood spots are an important and precious resource for genomic research. Whole genome amplification of dried blood spot DNA has been used to provide DNA for genome-wide SNP genotyping. Here we describe a 96 well format procedure to extract DNA from a portion of a dried blood spot that provides sufficient unamplified genomic DNA for genome-wide single nucleotide polymorphism (SNP) genotyping. We show that SNP genotyping of the unamplified DNA is more robust than genotyping amplified dried blood spot DNA, is comparable in cost, and can be done with thousands of samples. This procedure can be used for genome-wide association studies and other large-scale genomic analyses that require robust, high-accuracy genotyping of dried blood spot DNA.
authors.affiliation: Department of Biochemistry and Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Krystal R
authors.initials: KR
authors.lastname: St Julien
authors.forename: Laura L
authors.initials: LL
authors.lastname: Jelliffe-Pawlowski
authors.forename: Gary M
authors.initials: GM
authors.lastname: Shaw
authors.forename: David K
authors.initials: DK
authors.lastname: Stevenson
authors.forename: Hugh M
authors.initials: HM
authors.lastname: O'Brodovich
authors.forename: Mark A
authors.initials: MA
authors.lastname: Krasnow
mesh_terms: D004247:DNA / Q000235:genetics* / Q000302:isolation & purification; D059788:Dried Blood Spot Testing; D015894:Genome, Human / Q000235:genetics*; D055106:Genome-Wide Association Study; D060005:Genotyping Techniques / Q000191:economics / Q000379:methods*; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D020641:Polymorphism, Single Nucleotide; D011786:Quality Control; D013997:Time Factors
chemical_list: D004247:DNA