title: Swollenin aids in the amorphogenesis step during the enzymatic hydrolysis of pretreated biomass.
abstract: A key limitation in the overall hydrolysis process is the restricted access that the hydrolytic enzymes have due to the macro-and-micro structure of cellulose and its association with hemicellulose and lignin. Previous work has shown that several non-hydrolytic proteins can disrupt cellulose structure and boost the activity of hydrolytic enzymes when purer forms of cellulose are used. In the work reported here, Swollenin primarily disrupted the hemicellulosic fraction of pretreated corn stover, resulting in the solubilisation of monomeric and oligomeric sugars. Although Swollenin showed little synergism when combined with the cellulase monocomponents exoglucanase (CEL7A) and endoglucanase (CEL5A), it showed pronounced synergism with xylanase monocomponents Xylanase GH10 and Xylanase GH11, resulting in the release of significantly more xylose (>300%). It appears that Swollenin plays a role in amorphogenesis and that its primary action is enhancing access to the hemicellulose fraction that limits or masks accessibility to the cellulose component of lignocellulosic substrates.
authors.affiliation: Forest Products Biotechnology/Bioenergy Group, Department of Wood Science, Faculty of Forestry, University of British Columbia, 2424 Main Mall, Vancouver, BC, Canada V6T 1Z4.
authors.forename: Keith
authors.initials: K
authors.lastname: Gourlay
authors.forename: Jinguang
authors.initials: J
authors.lastname: Hu
authors.forename: Valdeir
authors.initials: V
authors.lastname: Arantes
authors.forename: Martina
authors.initials: M
authors.lastname: Andberg
authors.forename: Markku
authors.initials: M
authors.lastname: Saloheimo
authors.forename: Merja
authors.initials: M
authors.lastname: Penttilä
authors.forename: Jack
authors.initials: J
authors.lastname: Saddler
mesh_terms: D018533:Biomass; D002480:Cellulase / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D004586:Electrophoresis / Q000379:methods; D005656:Fungal Proteins / Q000378:metabolism*; D006868:Hydrolysis; D009024:Morphogenesis; D014995:Xylosidases / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D014995:Xylosidases; D002480:Cellulase
keywords: 8-aminonaphthalene-1,3,6-trisulfonic acid; ANTS; Biofuel; Biorefinery; CBM; HMW; Hemicellulase; Hemicellulose; PACE; SPCS; Swollenin; carbohydrate binding module; high molecular weight; polyacrylamide carbohydrate electrophoresis; steam pretreated corn stover