title: Alteration of the content of primary and secondary metabolites in strawberry fruit by Colletotrichum nymphaeae infection.
abstract: The effects of infection with Colletotrichum nymphaeae , the causative agent of strawberry black spot, were studied on two strawberry cultivars: susceptible 'Elsanta' and tolerant 'Honeoye' cultivars. Four treatments were established: (1) artificial inoculation; (2) spray application of pyraclostrobin + boscalid (Signum); (3) foliar spraying with calcium (Stopit); and (4) control (spraying with water). Primary metabolites (sugars and organic acids) and secondary metabolites (phenolic compounds) were determined in strawberry fruit with the use of HPLC-MS(n). Infected fruit accumulated large amounts of total sugars and low levels of organic acids. The sugar/acid ratio was higher in the infected and in Ca-treated strawberries. The contents of ellagic acid derivatives, flavonols, oligomeric procyanidins, flavan-3-ols, and total phenolics were highest in inoculated strawberry fruit. Results indicated that fungicide and calcium sprayings did not alter polyphenolic levels in plant tissue.
authors.affiliation: Chair for Fruit, Wine and Vegetable Growing, Department of Agronomy, Biotechnical Faculty, University of Ljubljana , Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia.
authors.forename: Maja
authors.initials: M
authors.lastname: Mikulic-Petkovsek
authors.forename: Valentina
authors.initials: V
authors.lastname: Schmitzer
authors.forename: Ana
authors.initials: A
authors.lastname: Slatnar
authors.forename: Nika
authors.initials: N
authors.lastname: Weber
authors.forename: Robert
authors.initials: R
authors.lastname: Veberic
authors.forename: Franci
authors.initials: F
authors.lastname: Stampar
authors.forename: Alenka
authors.initials: A
authors.lastname: Munda
authors.forename: Darinka
authors.initials: D
authors.lastname: Koron
mesh_terms: D000975:Antioxidants / Q000032:analysis / Q000378:metabolism; D050260:Carbohydrate Metabolism; D002241:Carbohydrates / Q000032:analysis; D020231:Colletotrichum / Q000502:physiology*; D004610:Ellagic Acid / Q000032:analysis / Q000378:metabolism; D031985:Fragaria / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism* / Q000382:microbiology*; D005638:Fruit / Q000737:chemistry* / Q000187:drug effects / Q000378:metabolism / Q000382:microbiology; D005659:Fungicides, Industrial / Q000494:pharmacology; D010935:Plant Diseases / Q000382:microbiology* / Q000517:prevention & control; D010936:Plant Extracts / Q000032:analysis / Q000378:metabolism; D064210:Secondary Metabolism
chemical_list: D000975:Antioxidants; D002241:Carbohydrates; D005659:Fungicides, Industrial; D010936:Plant Extracts; D004610:Ellagic Acid