title: Identification of ABC transporter genes in gonad tissue of two Mediterranean sea urchin species: black, Arbacia lixula L., and rocky, Paracentrotus lividus L.
abstract: Multixenobiotic resistance (MXR) represents an important cellular detoxification mechanism in aquatic organisms as it provides them robustness toward natural and man-made contaminants. Several ABC transporters have major roles in the MXR phenotype - P-gp/ABCB1, MRP1-3/ABCC1-3 and BCRP/ABCG2. In this study, we identified the presence of ABC transporters involved in the MXR mechanism of Arbacia lixula and Paracentrotus lividus. AlABCB1/P-gp, AlABCC3/MRP3, AlABCC9/SUR-like and AlABCG-like transcripts were identified in A. lixula; and PlABCC1/P-gp, PlABCC3/MRP3, PlABCC5/MRP5, and PlABCC9/SUR-like transcripts in P. lividus. For each of the new partial sequences, we performed detailed phylogenetic and identity analysis as a first step toward full characterization and understanding of the ecotoxicological role of these ABC transporters.
authors.affiliation: Laboratory for Biology and Microbial Genetics, Department of Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000, Zagreb, Croatia, ibosnjak@pbf.hr.
authors.forename: Ivana
authors.initials: I
authors.lastname: Bošnjak
authors.forename: Roko
authors.initials: R
authors.lastname: Zaja
authors.forename: Roberta Sauerborn
authors.initials: RS
authors.lastname: Klobučar
authors.forename: Lidija
authors.initials: L
authors.lastname: Šver
authors.forename: Jasna
authors.initials: J
authors.lastname: Franekić
authors.forename: Tvrtko
authors.initials: T
authors.lastname: Smital
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism; D018528:ATP-Binding Cassette Transporters / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D047308:Arbacia / Q000235:genetics* / Q000378:metabolism; D004784:Environmental Monitoring / Q000379:methods*; D006066:Gonads / Q000378:metabolism; D008522:Mediterranean Sea; D047328:Paracentrotus / Q000235:genetics* / Q000378:metabolism
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D018528:ATP-Binding Cassette Transporters