title: Efficient production of ethanol from empty palm fruit bunch fibers by fed-batch simultaneous saccharification and fermentation using Saccharomyces cerevisiae.
abstract: The concentration of ethanol produced from lignocellulosic biomass should be at least 40 g l(-1) [about 5 % (v/v)] to minimize the cost of distillation process. In this study, the conditions for the simultaneous saccharification and fermentation (SSF) at fed-batch mode for the production of ethanol from alkali-pretreated empty palm fruit bunch fibers (EFB) were investigated. Optimal conditions for the production of ethanol were identified as temperature, 30 °C; enzyme loading, 15 filter paper unit g(-1) biomass; and yeast (Saccharomyces cerevisiae) loading, 5 g l(-1) of dry cell weight. Under these conditions, an economical ethanol concentration was achieved within 17 h, which further increased up to 62.5 g l(-1) after 95 h with 70.6 % of the theoretical yield. To our knowledge, this is the first report to evaluate the economic ethanol production from alkali-pretreated EFB in fed-batch SSF using S. cerevisiae.
authors.affiliation: Applied Microbiology Research Center, Bio-Materials Research Institute, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup, Jeonbuk, 580-185, South Korea.
authors.forename: Jang Min
authors.initials: JM
authors.lastname: Park
authors.forename: Baek-Rock
authors.initials: BR
authors.lastname: Oh
authors.forename: Jeong-Woo
authors.initials: JW
authors.lastname: Seo
authors.forename: Won-Kyung
authors.initials: WK
authors.lastname: Hong
authors.forename: Anna
authors.initials: A
authors.lastname: Yu
authors.forename: Jung-Hoon
authors.initials: JH
authors.lastname: Sohn
authors.forename: Chul Ho
authors.initials: CH
authors.lastname: Kim
mesh_terms: D000468:Alkalies / Q000737:chemistry*; D029064:Araceae / Q000737:chemistry* / Q000382:microbiology*; D061249:Batch Cell Culture Techniques / Q000379:methods; D019149:Bioreactors / Q000382:microbiology; D050260:Carbohydrate Metabolism; D049109:Cell Proliferation; D002482:Cellulose / Q000378:metabolism; D000431:Ethanol / Q000302:isolation & purification / Q000378:metabolism*; D005285:Fermentation; D005638:Fruit / Q000737:chemistry* / Q000382:microbiology*; D010936:Plant Extracts / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000502:physiology*; D013696:Temperature
chemical_list: D000468:Alkalies; D010936:Plant Extracts; D000431:Ethanol; D002482:Cellulose