title: Population genetic study of Fagopyrum tataricum from Western Himalaya using ISSR markers.
abstract: Inter-simple sequence repeat (ISSR) markers were used to analyze genetic diversity and relatedness of 15 germplasms of Fagopyrum tataricum. Samples representing 75 individuals were collected from a range of altitudes in the Western Himalaya. The 13 ISSR primers revealed 98.1% polymorphism among populations, whereas average polymorphism was extremely low (2.18%) within populations. The coefficient of population differentiation was 0.9750, with limited gene flow (N m) of 0.0128. The average PIC value of the ISSR markers was high (0.812), with a marker ratio of 0.65 and marker index of 6.66. The genetic diversity of F. tataricum significantly correlated with altitude and gene diversity, Shannon's index, and the percentage of polymorphic bands. The genetic diversity among populations showed broad genetic base and provided a developmental strategy for crop improvement.
authors.affiliation: Molecular Biology and Nanotechnology Laboratory, Center for Biotechnology, University of Allahabad, Allahabad, Uttar Pradesh, India, garimadixit2007@gmail.com.
authors.forename: Garima
authors.initials: G
authors.lastname: Kishore
authors.forename: Anjana
authors.initials: A
authors.lastname: Pandey
authors.forename: Rajendra
authors.initials: R
authors.lastname: Dobhal
authors.forename: Sanjay
authors.initials: S
authors.lastname: Gupta
mesh_terms: D000531:Altitude; D018744:DNA, Plant; D019612:Fagopyrum / Q000235:genetics*; D017343:Genes, Plant; D005819:Genetic Markers; D014644:Genetic Variation; D007194:India; D018895:Microsatellite Repeats; D010802:Phylogeny; D018515:Plant Leaves / Q000235:genetics*; D011110:Polymorphism, Genetic
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers