title: Protein-protein interactions between intermediate chains and the docking complex of Chlamydomonas flagellar outer arm dynein.
abstract: Outer arm dynein (OAD) is bound to specific loci on outer-doublet-microtubules by interactions at two sites: via intermediate chain 1 (IC1) and the outer dynein arm docking complex (ODA-DC). Studies using Chlamydomonas mutants have suggested that the individual sites have rather weak affinities for microtubules, and therefore strong OAD attachment to microtubules is achieved by their cooperation. To test this idea, we examined interactions between IC1, IC2 (another intermediate chain) and ODA-DC using recombinant proteins. Recombinant IC1 and IC2 were found to form a 1:1 complex, and this complex associated with ODA-DC in vitro. Binding of IC1 to mutant axonemes revealed that there are specific binding sites for IC1. From these data, we propose a novel model of OAD-outer doublet association.
authors.affiliation: Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Ide
authors.forename: Mikito
authors.initials: M
authors.lastname: Owa
authors.forename: Stephen M
authors.initials: SM
authors.lastname: King
authors.forename: Ritsu
authors.initials: R
authors.lastname: Kamiya
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Wakabayashi
mesh_terms: D000818:Animals; D054468:Axoneme / Q000737:chemistry*; D001665:Binding Sites; D016825:Chlamydomonas reinhardtii / Q000166:cytology*; D002846:Chromatography, Affinity; D004398:Dyneins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D005407:Flagella / Q000378:metabolism*; D010940:Plant Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000302:isolation & purification; D011485:Protein Binding; D025941:Protein Interaction Mapping; D061987:Sf9 Cells; D018411:Spodoptera
chemical_list: D010940:Plant Proteins; D004398:Dyneins