title: A comparison of composition and emulsifying properties of MFGM materials prepared from different dairy sources by microfiltration.
abstract: Milk fat globule membrane (MFGM), due to its specific nature and composition, is known as material possessing advantageous nutritional as well as technological properties. In this study MFGM materials were produced from several dairy sources such as buttermilk (BM), butter serum (BS) and buttermilk whey (BMW) by microfiltration (MF). The obtained materials, depending on the sources, were called BM-MFGM, BS-MFGM and BMW-MFGM, respectively. The compositions of starting materials and the isolated MFGM materials as well as their emulsifying properties were analyzed and compared. As expected, the MF resulted in enrichment of polar lipids (PLs), major components of MFGM. On dry matter basis, BM-MFGM and BS-MFGM were about 2.5 times higher in PLs compared to their beginning materials while BMW-MFGM was about 8.3 times compared to buttermilk powder (BMP). Sodium dodecyl sulfate polyacrylamide gel electrophoresis showed that the microfiltered products still contained a high amount of non-MFGM proteins such as caseins, β-lactoglobulin, and α-lactalbumin. Emulsions of 35% soya oil in water were prepared with the mentioned materials using a homogenizer at various pressures. Generally, emulsions prepared with BMP and butter serum powder had significantly higher particle sizes than those prepared with the MFGM materials. This result along with microscopy observation and viscosity measurement indicated the presence of aggregated particles in the former emulsions, probably as a result of lack of surface-active components. The differences in composition, especially in content of PLs and proteins of the materials were the main reasons for the differences in their emulsifying behaviors.
authors.affiliation: Faculty of Agriculture, Institute of Food Technology and Biochemistry, University of Belgrade, Belgrade, Serbia jmiocin@agrif.bg.ac.rs.
authors.forename: Jelena
authors.initials: J
authors.lastname: Miocinovic
authors.affiliation: Faculty of Food Science and Technology, Nong Lam University, Ho Chi Minh, Vietnam.
authors.forename: Thien
authors.initials: T
authors.lastname: Le Trung
authors.affiliation: Laboratory of Food Technology and Engineering, Department of Food Safety and Food Quality, Faculty of Bioscience Engineering, Ghent University, Gent, Belgium.
authors.forename: Eveline
authors.initials: E
authors.lastname: Fredrick
authors.affiliation: Particle and Interfacial Technology Group, Faculty of Bioscience Engineering, Ghent University, Gent, Belgium.
authors.forename: Paul
authors.initials: P
authors.lastname: Van der Meeren
authors.affiliation: Faculty of Agriculture, Institute of Food Technology and Biochemistry, University of Belgrade, Belgrade, Serbia.
authors.forename: Predrag
authors.initials: P
authors.lastname: Pudja
authors.affiliation: Laboratory of Food Technology and Engineering, Department of Food Safety and Food Quality, Faculty of Bioscience Engineering, Ghent University, Gent, Belgium.
authors.forename: Koen
authors.initials: K
authors.lastname: Dewettinck
mesh_terms: D002079:Butter; D043302:Cultured Milk Products; D004655:Emulsions / Q000032:analysis*; D005374:Filtration / Q000379:methods*; D005511:Food Handling / Q000379:methods*; D006017:Glycolipids / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry*; D006801:Humans; D066292:Lipid Droplets; D008055:Lipids / Q000032:analysis; D008566:Membranes / Q000737:chemistry; D008894:Milk Proteins / Q000032:analysis; D010316:Particle Size; D013024:Soybean Oil; D000067796:Whey
chemical_list: D004655:Emulsions; D006017:Glycolipids; D006023:Glycoproteins; D008055:Lipids; D008894:Milk Proteins; C110367:milk fat globule; D013024:Soybean Oil; D002079:Butter
keywords: Milk fat globule membrane; butter serum; buttermilk; buttermilk whey; emulsion; microfiltration