title: Characterization of bacterial communities in hybrid upflow anaerobic sludge blanket (UASB)-membrane bioreactor (MBR) process for berberine antibiotic wastewater treatment.
abstract: Biodegradation of berberine antibiotic was investigated in upflow anaerobic sludge blanket (UASB)-membrane bioreactor (MBR) process. After 118days of operation, 99.0%, 98.0% and 98.0% overall removals of berberine, COD and NH4(+)-N were achieved, respectively. The detailed composition of the established bacterial communities was studied by using 16S rDNA clone library. Totally, 400 clones were retrieved and grouped into 186 operational taxonomic units (OTUs). UASB was dominated by Firmicutes and Bacteroidetes, while Proteobacteria, especially Alpha- and Beta-proteobacteria were prevalent in the MBRs. Clostridium, Eubacterium and Synergistes in the UASB, as well as Hydrogenophaga, Azoarcus, Sphingomonas, Stenotrophomonas, Shinella and Alcaligenes in the MBRs were identified as potential functional species in biodegradation of berberine and/or its metabolites. The bacterial community compositions in two MBRs were significantly discrepant. However, the identical functions of the functional species ensured the comparable pollutant removal performances in two bioreactors.
authors.affiliation: State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Dayangfang 8, Anwai Beiyuan, Beijing 100012, China.
authors.forename: Guanglei
authors.initials: G
authors.lastname: Qiu
authors.forename: Yong-Hui
authors.initials: YH
authors.lastname: Song
authors.forename: Ping
authors.initials: P
authors.lastname: Zeng
authors.forename: Liang
authors.initials: L
authors.lastname: Duan
authors.forename: Shuhu
authors.initials: S
authors.lastname: Xiao
mesh_terms: D000693:Anaerobiosis; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D001599:Berberine / Q000302:isolation & purification* / Q000378:metabolism; D019149:Bioreactors; D008567:Membranes, Artificial; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012722:Sewage; D062065:Wastewater; D014874:Water Pollutants, Chemical / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D001599:Berberine; D008567:Membranes, Artificial; D012722:Sewage; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: 16S rDNA clone library; Bacterial community structure; Berberine wastewater; Membrane bioreactor (MBR); Upflow anaerobic sludge blanket (UASB)