title: Electrochemical and spectroscopic investigations of carboxylic acid ligand and its triorganotin complexes for their binding with ds.DNA: in vitro biological studies.
abstract: A carboxylic acid ligand, (Z)-4-(4-acetylphenylamino)-4-oxobut-2-enoic acid (APA-1), and its triphenyl-(APA-2) and tributyl-tin(IV) (APA-3) compounds have been synthesized and investigated for their binding with ds.DNA using UV-visible spectroscopy, fluorescence spectroscopy, cyclic voltammetry, and viscosity measurements under physiological conditions of pH and temperature. The experimental results from all techniques i.e. binding constant (Kb), binding site size (n) and free energy change (ΔG) were in good agreement and inferred spontaneous compound-DNA complexes formation via intercalation. Among all the compounds APA-3 showed comparatively greater binding at pH 4.7 as evident from its greater Kb values {APA-3: Kb: 5.63×10(4)M(-1) (UV); 7.94×10(4)M(-1) (fluorescence); 9.91×10(4)M(-1) (CV)}. Electrochemical processes of compounds before and after the addition of DNA were found diffusion controlled. Among all compounds, APA-3 exhibited best antitumor activity.
authors.affiliation: Department of Chemistry, Allama Iqbal Open University, Islamabad, Pakistan. nasimaa2006@yahoo.com
authors.forename: Nasima
authors.initials: N
authors.lastname: Arshad
authors.forename: Shahid Iqbal
authors.initials: SI
authors.lastname: Farooqi
authors.forename: Moazzam H
authors.initials: MH
authors.lastname: Bhatti
authors.forename: Samreen
authors.initials: S
authors.lastname: Saleem
authors.forename: Bushra
authors.initials: B
authors.lastname: Mirza
mesh_terms: D016960:Agrobacterium tumefaciens; D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology; D002264:Carboxylic Acids / Q000737:chemistry; D049109:Cell Proliferation / Q000187:drug effects; D056831:Coordination Complexes / Q000737:chemistry* / Q000494:pharmacology; D004247:DNA / Q000737:chemistry*; D004563:Electrochemistry; D008024:Ligands; D009947:Organotin Compounds / Q000737:chemistry* / Q000494:pharmacology; D010941:Plant Tumors / Q000209:etiology; D011198:Solanum tuberosum; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014783:Viscosity
chemical_list: D000970:Antineoplastic Agents; D002264:Carboxylic Acids; D056831:Coordination Complexes; D008024:Ligands; D009947:Organotin Compounds; D004247:DNA
keywords: Binding constant/site size; Bioactivities; Cyclic voltammerty; Intercalation; Triorganotin(IV) carboxylates; UV–visible/fluorescence spectroscopy