title: Metabolic engineering of Escherichia coli for enhanced biosynthesis of poly(3-hydroxybutyrate) based on proteome analysis.
abstract: We have previously analyzed the proteome of recombinant Escherichia coli producing poly(3-hydroxybutyrate) [P(3HB)] and revealed that the expression level of several enzymes in central metabolism are proportional to the amount of P(3HB) accumulated in the cells. Based on these results, the amplification effects of triosephosphate isomerase (TpiA) and fructose-bisphosphate aldolase (FbaA) on P(3HB) synthesis were examined in recombinant E. coli W3110, XL1-Blue, and W lacI mutant strains using glucose, sucrose and xylose as carbon sources. Amplification of TpiA and FbaA significantly increased the P(3HB) contents and concentrations in the three E. coli strains. TpiA amplification in E. coli XL1-Blue lacI increased P(3HB) from 0.4 to 1.6 to g/l from glucose. Thus amplification of glycolytic pathway enzymes is a good strategy for efficient production of P(3HB) by allowing increased glycolytic pathway flux to make more acetyl-CoA available for P(3HB) biosynthesis.
authors.affiliation: Industrial Biochemicals Research Group, Division of Convergence Chemistry, Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology, P.O. Box 107, 141 Gajeong-ro, Yuseong-gu, Daejeon, 305-600, Republic of Korea.
authors.forename: Seung Hwan
authors.initials: SH
authors.lastname: Lee
authors.forename: Kyoung-Hee
authors.initials: KH
authors.lastname: Kang
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Kim
authors.forename: Tong Un
authors.initials: TU
authors.lastname: Chae
authors.forename: Young Hoon
authors.initials: YH
authors.lastname: Oh
authors.forename: Soon Ho
authors.initials: SH
authors.lastname: Hong
authors.forename: Bong Keun
authors.initials: BK
authors.lastname: Song
authors.forename: Jonggeon
authors.initials: J
authors.lastname: Jegals
authors.forename: Si Jae
authors.initials: SJ
authors.lastname: Park
authors.forename: Sang Yup
authors.initials: SY
authors.lastname: Lee
mesh_terms: D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000032:analysis; D015870:Gene Expression; D006885:Hydroxybutyrates / Q000378:metabolism*; D060847:Metabolic Engineering; D053858:Metabolic Networks and Pathways / Q000235:genetics; D011091:Polyesters / Q000378:metabolism*; D020543:Proteome / Q000032:analysis*; D000094122:Polyhydroxybutyrates
chemical_list: D029968:Escherichia coli Proteins; D006885:Hydroxybutyrates; D011091:Polyesters; D020543:Proteome; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates