title: [Localization and function of CD59 and Cbp in T lymphocytes].
abstract: OBJECTIVE
  To investigate the distributions of GPI-anchored protein CD59 and C-terminal Src kinase-binding protein (Cbp) in cell membrane of T lymphocytes and the roles in cell activation and proliferation.
  
  
  METHODS
  The locations of CD59 and Cbp were observed under a fluorescence microscope with the immunofluorescence cytochemistry. The Jurkat cells were transfected with the recombinant plasmid pSUPER-siCD59 using the electric transfection method. Using RT-PCR and Western blotting, we detected the expression of CD59 and the phosphorylation level of protein-tyrosine kinase oncogene family (fyn) in different groups. Moreover, the cell proliferation activity was measured by MTT assay.
  
  
  RESULTS
  The immunofluorescence cytochemistry showed that CD59 and Cbp were mainly distributed in cell membrane. Compared with other groups, the quantitative RT-PCR and Western blotting showed that the expression of CD59 was reduced, and along with it the phosphorylation level of fyn decreased in pSUPER-siCD59 group, and MTT assay revealed that its cell proliferation was inhibited (P<0.05).
  
  
  CONCLUSIONS
  CD59 was a type of membrane-bound protein, CD59 and Cbp played synergistic roles in cell activation and proliferation.
authors.affiliation: Department of Immunology, Medical College, Qingdao University, Hospital Affiliated to Medical College, Qingdao University, Qingdao 266071, China. meihuagao@yahoo.com.cn
authors.forename: Meihua
authors.initials: M
authors.lastname: Gao
authors.forename: Jing
authors.initials: J
authors.lastname: Ji
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.forename: Bei
authors.initials: B
authors.lastname: Zhang
authors.forename: Shuchao
authors.initials: S
authors.lastname: Zhang
authors.forename: Yun
authors.initials: Y
authors.lastname: Qin
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D017252:CD3 Complex / Q000378:metabolism; D018957:CD59 Antigens / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D018274:Electroporation; D015870:Gene Expression; D006801:Humans; D019169:Jurkat Cells; D008213:Lymphocyte Activation / Q000235:genetics / Q000276:immunology; D008565:Membrane Proteins / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics; D021381:Protein Transport; D012333:RNA, Messenger / Q000235:genetics; D013601:T-Lymphocytes / Q000276:immunology / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D017252:CD3 Complex; D018957:CD59 Antigens; D008565:Membrane Proteins; C498710:PAG1 protein, human; D012333:RNA, Messenger