title: Moniliformin analysis in maize samples from North-West Italy using multifunctional clean-up columns and the LC-MS/MS detection method.
abstract: A fast clean-up method has been developed to purify maize extracts and to detect moniliformin (MON) in maize samples from North-West Italy over a four-year period (2008-2011). The method is based on the use of MycoSep® 240 Mon clean-up columns (Romer Labs®). Samples were extracted using acetonitrile/water (84:16, v/v), and the extracts were purified with previously described clean-up columns. The liquid chromatography-tandem mass spectrography (LC-MS/MS) analysis has been carried out by means of hydrophilic interaction chromatography (HILIC), combined with negative electrospray mass spectrometry. The method has a recovery of 76-91% (relative standard deviation, RSD%: 6-14%), a limit of detection (LOD) of 1 µg kg⁻¹ and a limit of quantification (LOQ) of 4 µg kg⁻¹. Naturally contaminated maize (108 samples) was analysed for MON content. The average percentages of positive samples was 93% with the following ranges (µg kg⁻¹): 33-2606 (2008); <LOD-527 (2009); <LOD-920 (2010); <LOD-409 (2011).
authors.affiliation: Dipartimento di Scienze Agrarie, Forestali e Alimentari, University of Turin, Grugliasco, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Scarpino
authors.forename: Massimo
authors.initials: M
authors.lastname: Blandino
authors.forename: Michele
authors.initials: M
authors.lastname: Negre
authors.forename: Amedeo
authors.initials: A
authors.lastname: Reyneri
authors.forename: Francesca
authors.initials: F
authors.lastname: Vanara
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D003503:Cyclobutanes / Q000032:analysis*; D007558:Italy; D009183:Mycotoxins / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D003313:Zea mays / Q000737:chemistry*
chemical_list: D003503:Cyclobutanes; D009183:Mycotoxins; C008539:moniliformin