title: Gene structure of the two-domain taurocyamine kinase from Paragonimus westermani: evidence for a distinct lineage of trematode phosphagen kinases.
abstract: Taurocyamine kinase (TK) is an enzyme that catalyzes the reversible transfer of a phosphate between ATP and taurocyamine. Annelid TKs were suggested to have evolved from a CK ancestor. However, TKs from the lung fluke Paragonimus westermani comprised another lineage. Construction of phylogenetic tree and comparison of exon/intron organization showed that P. westermani TK and other trematode TKs evolved from a molluscan arginine kinase (AK) gene. Exon shuffling probably caused the changes in amino acid sequence thereby changing the affinity from AK to TK. The present study provides new insights on the evolution of phosphagen kinases found in trematodes.
authors.affiliation: Department of Environmental Health Sciences, Kochi University, Kochi 783-8505, Japan.
authors.forename: Blanca R
authors.initials: BR
authors.lastname: Jarilla
authors.forename: Shinji
authors.initials: S
authors.lastname: Tokuhiro
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Nagataki
authors.forename: Kouji
authors.initials: K
authors.lastname: Uda
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Suzuki
authors.forename: Luz P
authors.initials: LP
authors.lastname: Acosta
authors.forename: Takeshi
authors.initials: T
authors.lastname: Agatsuma
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D017124:Conserved Sequence; D019143:Evolution, Molecular; D005784:Gene Amplification; D015801:Helminth Proteins / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D048492:Paragonimus westermani / Q000201:enzymology*; D017852:Phosphotransferases (Nitrogenous Group Acceptor) / Q000737:chemistry / Q000235:genetics*; D010802:Phylogeny; D017434:Protein Structure, Tertiary; D017422:Sequence Analysis, DNA
chemical_list: D015801:Helminth Proteins; D017852:Phosphotransferases (Nitrogenous Group Acceptor); C022716:taurocyamine kinase