title: Exploration of tumor-suppressive microRNAs silenced by DNA hypermethylation in cervical cancer.
abstract: BACKGROUND
  Multiple studies proved that miRNAs have a causal role in tumorigenesis. Some miRNAs are regulated by epigenetic alterations in their promoter regions and can be activated by chromatin- modifying drugs.
  
  
  METHODS
  We treated cervical cancer cells with 5-aza-2'-deoxycytidine and get a microarray analysis. Dysregulation of miRNAs was measured by qPCR in cervical cell lines and methylation status of them in cervical cancer tissue were performed with MeDIP-qPCR assay.
  
  
  RESULTS
  We found hypermethylation of miR-432, miR-1286, miR-641, miR-1290, miR-1287 and miR-95 may have some relationship with HPV infection in cervical cell lines. In primary tumors of cervix with paired normal tissue, expression levels of miRNAs were inversely correlated with their DNA methylation status in the cervical cancer cell lines treated with 5-AZA.
  
  
  CONCLUSIONS
  Our results indicate that miRNAs might play a role in the pathogenesis of human cervical cancer with HPV and identify altered miRNA methylation as a possible epigenetic mechanism involved in their aberrant expression.
authors.affiliation: Department of Gynecological Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, 107 Yan Jiang West Road, Guangzhou 510120, People's Republic of China. yaoyaotingting@msn.com
authors.forename: Tingting
authors.initials: T
authors.lastname: Yao
authors.forename: Qunxian
authors.initials: Q
authors.lastname: Rao
authors.forename: Longyang
authors.initials: L
authors.lastname: Liu
authors.forename: Chengyu
authors.initials: C
authors.lastname: Zheng
authors.forename: Qingsheng
authors.initials: Q
authors.lastname: Xie
authors.forename: Jinxiao
authors.initials: J
authors.lastname: Liang
authors.forename: Zhongqiu
authors.initials: Z
authors.lastname: Lin
mesh_terms: D001374:Azacitidine / Q000031:analogs & derivatives / Q000378:metabolism; D045744:Cell Line, Tumor; D019175:DNA Methylation; D000077209:Decitabine; D004791:Enzyme Inhibitors / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D020868:Gene Silencing; D006801:Humans; D035683:MicroRNAs / Q000378:metabolism*; D046228:Microarray Analysis; D060888:Real-Time Polymerase Chain Reaction; D002583:Uterine Cervical Neoplasms / Q000473:pathology
chemical_list: D004791:Enzyme Inhibitors; D035683:MicroRNAs; D000077209:Decitabine; D001374:Azacitidine