title: Metabolic differentiations of Pueraria lobata and Pueraria thomsonii using ¹H NMR spectroscopy and multivariate statistical analysis.
abstract: Puerariae Radix was a widely used herbal medicine. Pueraria lobata (PL) and Pueraria thomsonii (PT) were the two authorized sources of Puerariae Radix (gegen) in China. In this study, metabolic differentiations between these two species were investigated using NMR spectroscopy followed by principal components analysis (PCA) and partial least squares-discriminant analysis (PLS-DA). The content of puerarin in PL and PT was also determined using quantitative (1)H NMR spectroscopy. Thirteen isoflavones were tentatively identified based on 1D and 2D NMR spectroscopic data in two species. The (1)H NMR spectra of PL and PT were obviously different. PL and PT could also be markedly discriminated from (1)H NMR spectroscopic data by PCA and PLS-DA. For the crude drug resources, isoflavones, in which puerarin is the most important one, were regarded as the reasonable markers for the discrimination of the two species. The contents of puerarin and total isoflavones in PL were quantitated much higher than those in PT. Above all, (1)H NMR spectroscopy, which can provide comprehensive profiles of the metabolites and achieve convenient determinations of puerarin and total isoflavones in a single run, is an efficient means for evaluating the medicinal samples and achieving a better quality control of Puerariae Radix.
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau 999078, Macao.
authors.forename: Yan-Gan
authors.initials: YG
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau 999078, Macao.
authors.forename: Yue-Lin
authors.initials: YL
authors.lastname: Song
authors.affiliation: Institute of Traditional Chinese Medicine and Natural Products, Jinan University, Guangzhou 510632, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University 510642, Guangzhou, China.
authors.forename: Yun-Fei
authors.initials: YF
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau 999078, Macao; Institute of Traditional Chinese Medicine and Natural Products, Jinan University, Guangzhou 510632, China.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Huang
authors.affiliation: Institute of Traditional Chinese Medicine and Natural Products, Jinan University, Guangzhou 510632, China.
authors.forename: Wen-Cai
authors.initials: WC
authors.lastname: Ye
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau 999078, Macao.
authors.forename: Yi-Tao
authors.initials: YT
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau 999078, Macao. Electronic address: qwzhang@umac.mo.
authors.forename: Qing-Wen
authors.initials: QW
authors.lastname: Zhang
mesh_terms: D002681:China; D016002:Discriminant Analysis; D007529:Isoflavones / Q000737:chemistry / Q000302:isolation & purification*; D016018:Least-Squares Analysis; D015999:Multivariate Analysis; D018517:Plant Roots; D025341:Principal Component Analysis; D066244:Proton Magnetic Resonance Spectroscopy / Q000379:methods*; D029908:Pueraria / Q000737:chemistry*; D011786:Quality Control; D013045:Species Specificity
chemical_list: D007529:Isoflavones; C033607:puerarin
keywords: Nuclear magnetic resonance; Pueraria lobata; Pueraria thomsonii; Puerariae Radix; Puerarin