title: Double transgenic mice--a suitable model for studying oxidative stress in type 1 diabetes mellitus.
abstract: Type 1 diabetes mellitus (T1DM), one of the most prevalent chronic diseases is characterized by the progressive destruction of pancreatic beta cells, leading to insulin deficiency and hyperglycemia. Studies performed on diabetic subjects with prolonged hyperglycemia showed the oxidative stress occurrence followed by molecular, cellular and tissue damage. Currently, reducing the oxidative stress represents a therapeutic target, in order to reduce its complications in diabetic patients. An adequate experimental model of type 1 diabetes represents a prerequisite in oxidative stress study, therefore, we assessed oxidative status in polymorphonuclear cells (PMNs) and peritoneal macrophages using a double transgenic (dTg) mouse model of type 1 diabetes. Our results revealed the increased production of reactive oxygen species (superoxide anion and H2O2) and nitrogen (nitric oxide) species in diabetic mice leading to the idea of oxidative stress model for the study of its complications in type 1 diabetes.
authors.affiliation: Cantacuzino National Institute of Research-Development for Microbiology and Immunology, Bucharest, Romania. aiancu@cantacuzino.ro
authors.forename: Adina Daniela
authors.initials: AD
authors.lastname: Iancu
authors.forename: Crina
authors.initials: C
authors.lastname: Stăvaru
mesh_terms: D000818:Animals; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D009504:Neutrophils / Q000378:metabolism; D009569:Nitric Oxide / Q000096:biosynthesis; D018384:Oxidative Stress; D013481:Superoxides / Q000378:metabolism
chemical_list: D013481:Superoxides; D009569:Nitric Oxide; D006861:Hydrogen Peroxide