title: [Simultaneous determination of five flavonoids and specific chromatograms analysis of huangkui capsule].
abstract: OBJECTIVE
  To Set up a method for determining the contents of the five flavonoids simultaneously in the HuangKui capsule and analyze their specific chromatograms.
  
  
  METHODS
  HPLC method was used. The analytical column was Thermo scientific Hypersil GOLD (250 mm x 4.6 mm, 5 microm). The mobile phase was composed of acetonitrile (A) and 0.2% orthophosphoric acid (B) with gradient elution. The flow rate was 0.8 mL/min and detection wavelength was 360 nm. The column temperature was maintained at 30 degrees C.
  
  
  RESULTS
  Contents of the five flavonoids (Rutin, Hyperoside, Isoquercitrin, Myricetin, Quercitrin) had good resolution with the correlation coefficients exceed 0.9999 and the average percent recovery lied in 98.46% to 100.33%. The chromatograms of the HuangKui capsule shared 15 common peaks in which 5 of them were recognized by the reference standard. Chromatograms of 10 lots of HuangKui capsule were analyzed with the similarities over 0.95.
  
  
  CONCLUSIONS
  The proposed method of contents determination and chromatogram analysis has strong characteristic and specificity. This method is fast, easy and reliable, and can be applied for quality control of the preparation.
authors.affiliation: Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China. liulin198951@126.com
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.forename: Hai-Tao
authors.initials: HT
authors.lastname: Tang
authors.forename: Han-Qing
authors.initials: HQ
authors.lastname: Liu
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: Zhang
authors.forename: Yun
authors.initials: Y
authors.lastname: Tang
mesh_terms: D028483:Abelmoschus / Q000737:chemistry*; D002214:Capsules; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000592:standards; D005419:Flavonoids / Q000032:analysis*; D011786:Quality Control; D011794:Quercetin / Q000031:analogs & derivatives / Q000032:analysis; D015203:Reproducibility of Results; D012431:Rutin / Q000032:analysis
chemical_list: D002214:Capsules; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; C016527:isoquercitrin; C012526:quercitrin; D012431:Rutin; C040015:myricetin; C021304:hyperoside; D011794:Quercetin