title: Proteomic analysis of normal murine brain parts.
abstract: Murine brain is an excellent tool for studying protein expression and brain function in mammals. Although mice are an extensively used model to recapitulate various pathological conditions, the proteome of the normal mouse brain has not been yet reported. In the present study, we identified the total proteins of different parts of the brain of CB7BL/6 mice, a widely used strain, by applying proteomic methodologies. The adult mouse brain was dissected anatomically into the following regions: frontal cortex, olfactory bulb, hippocampus, midbrain, cerebellum, hypothalamus and medulla. Total protein extracts of these regions were separated by two-dimensional gel electrophoresis and analyzed by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry, following in-gel digestion with trypsin. Protein identification was carried out by peptide mass fingerprint. Thus, 515 different single-gene products were identified in total, 54 expressed specifically in the olfactory bulb, 62 in the hippocampus, 36 in the frontal cortex, five in the cerebellum, nine in the midbrain, eight in the hypothamamus and 10 in the medulla. The majority of the proteins were enzymes, structural proteins and transporters. Moreover, the distribution of these molecules appears to exhibit direct correlation with the function of the brain regions where they were expressed. This study leads to the complete characterization of the normal mouse brain proteome as well as the protein expression profile of the different brain regions. These results will aid in addressing unmet scientific needs regarding physiological and pathological brain functions.
authors.affiliation: Division of Biotechnology, Centre of Basic Research II, Biomedical Research Foundation of the Academy of Athens, Athens, Greece.
authors.forename: Vasiliki K
authors.initials: VK
authors.lastname: Taraslia
authors.forename: Alexandros
authors.initials: A
authors.lastname: Kouskoukis
authors.forename: Athanasios K
authors.initials: AK
authors.lastname: Anagnostopoulos
authors.forename: Dimitrios J
authors.initials: DJ
authors.lastname: Stravopodis
authors.forename: Lukas H
authors.initials: LH
authors.lastname: Margaritis
authors.forename: George Th
authors.initials: GT
authors.lastname: Tsangaris
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D042541:Intracellular Space / Q000378:metabolism; D051379:Mice; D021381:Protein Transport; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics / Q000379:methods
chemical_list: D020543:Proteome
keywords: Proteome; cerebellum; frontal cortex; hippocampus; hypothalamus; medulla; midbrain; normal mouse brain; olfactory bulb; proteomics