title: Characterization and expression analysis of three CBF/DREB1 transcriptional factor genes from mangrove Avicennia marina.
abstract: Three CBF/DREB1 (C-repeat binding factor/dehydration responsive element-binding factor 1) homologues were isolated from mangrove Avicennia marina and designated AmCBF1, 2 and 3. Multiple sequence analysis showed that the three deduced proteins all contain an AP2 DNA-binding domain and two CBF signature sequences. According to the phylogenetic analysis, these proteins belong to the A-1 subgroup of the DREB subfamily. Expression analyses based on quantitative real-time PCR revealed that the AmCBF2 displayed relatively high expression under normal conditions, with the highest level in stems, while both AmCBF1 and 3 were weakly expressed without stress. The three genes also showed different responses to various environmental stimuli. The AmCBF2 was inducible by cold, drought, high salinity, heavy metals, as well as abscisic acid (ABA), and exhibited much stronger induction by cold, drought, Pb(2+) or Zn(2+) than by NaCl, ABA or Cd(2+). In contrast, both AmCBF1 and AmCBF3 displayed insignificant changes under these stimuli. These results indicate that the three AmCBF genes play different roles in A. marina and the AmCBF2 might be involved in the signaling pathway of cold, drought and heavy metal stress response.
authors.affiliation: State Key Laboratory of Tropical Oceanography, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China.
authors.forename: Ya-Lan
authors.initials: YL
authors.lastname: Peng
authors.forename: You-Shao
authors.initials: YS
authors.lastname: Wang
authors.forename: Hao
authors.initials: H
authors.lastname: Cheng
authors.forename: Cui-Ci
authors.initials: CC
authors.lastname: Sun
authors.forename: Peng
authors.initials: P
authors.lastname: Wu
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Wang
authors.forename: Jiao
authors.initials: J
authors.lastname: Fei
mesh_terms: D000040:Abscisic Acid / Q000633:toxicity; D032407:Avicennia / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D004785:Environmental Pollutants / Q000633:toxicity*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D019216:Metals, Heavy / Q000633:toxicity; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017434:Protein Structure, Tertiary; D012492:Salts / Q000633:toxicity; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013312:Stress, Physiological / Q000235:genetics*; D013696:Temperature; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D004785:Environmental Pollutants; D019216:Metals, Heavy; D010940:Plant Proteins; D012492:Salts; D014157:Transcription Factors; D000040:Abscisic Acid
keywords: Abiotic stress; Avicennia marina; CBF/DREB1 transcription factor; Gene expression; Mangrove; Real-time quantitative PCR