title: Immunohistochemical detection of the latent nuclear antigen-1 of the human herpesvirus type 8 to differentiate cutaneous epidemic Kaposi sarcoma and its histological simulators.
abstract: Kaposi's sarcoma is the most common neoplasia diagnosed in AIDS patients and the expression of the human herpesvirus-8 (HHV-8) latent nuclear antigen-1 has been useful for its histological diagnosis. The aim of this study is to confirm that immunohistochemistry is a valuable tool for differentiating KS from its simulators in skin biopsies of HIV patients. Immunohistochemical and histological analyses were performed in 49 Kaposi's sarcoma skin biopsies and 60 of its histological simulators. Positivity was present in the 49 Kaposi's sarcoma skin biopsies and no staining was observed in the 60 simulators analyzed, resulting in sensibility and specificity of 100%. HHV-8 immunohistochemical detection is an effective tool for diagnosing Kaposi's sarcoma, especially in early lesions in which neoplastic features are not evident. It also contributes to its histological differential diagnosis.
authors.affiliation: Evandro Chagas Clinical Research Institute, Oswaldo Cruz Foundation (IPEC-FIOCRUZ), Rio de Janeiro, Brazil. drapatriciafonsecap@gmail.com
authors.forename: Patricia Fonseca
authors.initials: PF
authors.lastname: Pereira
authors.forename: Tullia
authors.initials: T
authors.lastname: Cuzzi
authors.forename: Maria Clara Gutierrez
authors.initials: MC
authors.lastname: Galhardo
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000276:immunology / Q000473:pathology*; D000163:Acquired Immunodeficiency Syndrome / Q000150:complications; D000328:Adult; D001706:Biopsy; D003937:Diagnosis, Differential; D005260:Female; D019288:Herpesvirus 8, Human / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D009687:Nuclear Proteins / Q000032:analysis*; D010750:Phosphoproteins / Q000032:analysis*; D012514:Sarcoma, Kaposi / Q000276:immunology / Q000473:pathology*; D012878:Skin Neoplasms / Q000276:immunology / Q000473:pathology*; D013997:Time Factors
chemical_list: D009687:Nuclear Proteins; D010750:Phosphoproteins; C118485:latent nuclear antigen (LNA)