title: Identification of a linear B-cell epitope within the Bluetongue virus serotype 8 NS2 protein using a phage-displayed random peptide library.
abstract: The NS2 protein of Bluetongue virus (BTV) is an important non-structural protein and plays important roles in viral replication and assembly. In this study, one monoclonal antibody (mAb), 4D4, was raised against BTV8 NS2. Phage display technology was used and identified the consensus binding motif SNYD recognized by mAb 4D4. To define the minimal region required for antibody binding, a panel of synthetic peptides encompassing SNYD derived from the BTV8 NS2 was then used to more specifically define the 4D4 epitope as (149)RSNYDV(154). Furthermore, amino acid sequence alignments of different BTV serotypes and other orbiviruses suggested that this epitope is highly conserved among the BTV serotypes. The mAb reagent generated in this study may be applied to the development of BTV diagnosis and surveillance programs and the epitope defined here can lead to important insights into how BTV might interact with the sheep's immune system.
authors.affiliation: The Key Laboratory of Veterinary Public Health, Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, PR China. yongliqin119@163.com
authors.forename: Yong-Li
authors.initials: YL
authors.lastname: Qin
authors.forename: En-Cheng
authors.initials: EC
authors.lastname: Sun
authors.forename: Ni-Hong
authors.initials: NH
authors.lastname: Liu
authors.forename: Tao
authors.initials: T
authors.lastname: Yang
authors.forename: Qing-Yuan
authors.initials: QY
authors.lastname: Xu
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.forename: Wen-Shi
authors.initials: WS
authors.lastname: Wang
authors.forename: Peng
authors.initials: P
authors.lastname: Wei
authors.forename: Yu-Fei
authors.initials: YF
authors.lastname: Feng
authors.forename: Jun-Ping
authors.initials: JP
authors.lastname: Li
authors.forename: Dong-Lai
authors.initials: DL
authors.lastname: Wu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001820:Bluetongue virus / Q000145:classification* / Q000276:immunology*; D002460:Cell Line; D006224:Cricetinae; D004797:Enzyme-Linked Immunosorbent Assay; D018985:Epitopes, B-Lymphocyte / Q000276:immunology*; D019151:Peptide Library; D016415:Sequence Alignment; D017361:Viral Nonstructural Proteins / Q000276:immunology*
chemical_list: D018985:Epitopes, B-Lymphocyte; D019151:Peptide Library; D017361:Viral Nonstructural Proteins