title: Enhanced in vitro antitumor activity of a titanocene complex encapsulated into polycaprolactone (PCL) electrospun fibers.
abstract: OBJECTIVE
  The purpose of this work was to achieve detailed biomaterials characterization of a drug delivery system for local cancer treatment based on electrospun titanocene trichloride-loaded resorbable polycaprolactone (PCL) fibers.
  
  
  METHODS
  The PCL fibers were characterized for their structural, morphologic and physical properties. The drug release kinetics of the titanocene complex was investigated at different concentrations, to obtain a set of correlations between structure and tuneable release. After exposing cancer cells directly onto the surface of PCL fibers, the anti-proliferative effects of titanocene-loaded PCL were assessed by: (i) counting viable cells via live/dead staining methods, and (ii) analyzing cell apoptosis.
  
  
  CONCLUSIONS
  Titanocene concentration influenced fiber diameters reduced for PCL filled with titanocene. X-ray analysis suggested that the titanocene, encapsulated into the PCL fibers, is not allowed to crystallize and exists as amorphous aggregates into the fibers. The titanocene release curves presented two stages unrelated to PCL degradation: an initial burst release followed by a release linear with time, extending for a very long time. All of the titanocene-loaded fibers revealed sustained drug release properties suggesting their potential clinical applicability for the treatment of local cancer diseases.
authors.affiliation: Department of Industrial Engineering, University of Salerno, Fisciano, Salerno - Italy.
authors.forename: Mariamelia
authors.initials: M
authors.lastname: Stanzione
authors.forename: Orsolina
authors.initials: O
authors.lastname: Petillo
authors.forename: Anna
authors.initials: A
authors.lastname: Calarco
authors.forename: Eduardo
authors.initials: E
authors.lastname: Valarezo
authors.forename: Mariagrazia
authors.initials: M
authors.lastname: Napoli
authors.forename: Pasquale
authors.initials: P
authors.lastname: Longo
authors.forename: Francesco
authors.initials: F
authors.lastname: Riccitiello
authors.forename: Vittoria
authors.initials: V
authors.lastname: Vittoria
authors.forename: Gianfranco
authors.initials: G
authors.lastname: Peluso
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000493:pharmacokinetics; D002470:Cell Survival / Q000187:drug effects; D003692:Delayed-Action Preparations; D004339:Drug Compounding; D055664:Electrochemical Techniques; D005909:Glioblastoma / Q000473:pathology; D006801:Humans; D057139:Nanofibers / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods; D009942:Organometallic Compounds / Q000008:administration & dosage* / Q000493:pharmacokinetics; D011091:Polyesters / Q000737:chemistry*; D014407:Tumor Cells, Cultured
chemical_list: D000970:Antineoplastic Agents; D003692:Delayed-Action Preparations; D009942:Organometallic Compounds; D011091:Polyesters; C406884:titanocene; C016240:polycaprolactone