title: In vitro antioxidant, cytotoxic, thrombolytic activities and phytochemical evaluation of methanol extract of the A. philippense L. leaves.
abstract: OBJECTIVE
  To study the leaves of Adiantum philippense L. for their antioxidant, cytotoxicity and thrombolytic activities and to perform phytochemical evaluation.
  
  
  METHODS
  In-vitro antioxidant activity of extract was studied using DPPH radical scavenging, reducing power, total phenol and total flavonoid content determination assays. The cytotoxic activity was determined using brine shrimp lethality bioassay, thrombolytic activity by clot disruption and phytochemical potential by qualitative analysis.
  
  
  RESULTS
  The antioxidant activity of the extracts was found promising. The reducing power of this crude extract increase with the increase of concentration; IC50 values of DPPH scavenging activity was (140.00±0.86) µg/mL as compared to ascorbic acid [IC50 (130.00±0.76) µg/mL]; Total phenol and total flavonoids content were (148.26±0.24) mg/mL and (163.06±0.56) mg/mL respectively. In cytotoxicity assay the LC50 values of the sample was (106.41±0.78) µg/mL where as for standard vincristin sulphate was (08.50±0.24) µg/mL as a positive control and the extract shows (12.86±1.02)% clot lytic whereas standard streptokinase shows (30.86±0.44% clot lytic activity in thrombolytic assay. The phytochemical evaluation indicates the presence of chemical constituents including carbohydrates, alkaloids, saponins, glycosides, flavonoids.
  
  
  CONCLUSIONS
  This study shows that the methanol extract of leaves of Adiantum philippense L. has bioactivity but further compound isolation is necessary to confirm the activities of individual compounds.
authors.affiliation: Department of Pharmacy, International Islamic University Chittagong, Chittagong-4203, Bangladesh.
authors.forename: Mohammad Sekendar
authors.initials: MS
authors.lastname: Ali
authors.forename: Mohammad Ruhul
authors.initials: MR
authors.lastname: Amin
authors.forename: Chowdhury Mohammad Imtiaz
authors.initials: CM
authors.lastname: Kamal
authors.forename: Mohammad Aslam
authors.initials: MA
authors.lastname: Hossain
mesh_terms: D035681:Adiantum / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D001156:Artemia / Q000187:drug effects*; D001777:Blood Coagulation / Q000187:drug effects*; D005343:Fibrinolytic Agents / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity*; D008610:Menthol / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D018515:Plant Leaves / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D005343:Fibrinolytic Agents; D010936:Plant Extracts; D008610:Menthol
keywords: Adiantum philippense L.; Antioxidant; Cytotoxicity; Phytochemical evaluation