title: Caspr interaction with Amyloid Precursor Protein reduces amyloid-β generation in vitro.
abstract: Contactin associated protein (Caspr), an adhesion molecule, plays roles in formation of paranodal junctions in myelinated axons, neurite outgrowth, synaptic plasticity in nervous system. Here we have shown a novel function of Caspr in pathogenesis of Alzheimer's disease (AD). Caspr distributes around amyloid plaques in APP/PS1 mice. Levels of Caspr increase in the cerebral cortex of 7-month-old APP/PS1 mice comparing to wild-type littermates. Caspr decreased protein levels of APP in both HEK-293 cells stably transfected with Indiana mutant APP (V717F; HEK-APP) and CHO cells which express endogenous APP, while it did not alter mRNA levels of APP. Furthermore, Caspr co-localizes and interacts with APP. Amyloid-β (Aβ) 40 and Aβ42 generation were also reduced in HEK-APP cells by Caspr overexpression.
authors.affiliation: Institute of Neuroscience, Soochow University, Suzhou 215123, China.
authors.forename: Liang-feng
authors.initials: LF
authors.lastname: Fan
authors.forename: De-en
authors.initials: DE
authors.lastname: Xu
authors.forename: Wei-hua
authors.initials: WH
authors.lastname: Wang
authors.forename: Ke
authors.initials: K
authors.lastname: Yan
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.forename: Xue-qin
authors.initials: XQ
authors.lastname: Yao
authors.forename: Ru-xiang
authors.initials: RX
authors.lastname: Xu
authors.forename: Chun-feng
authors.initials: CF
authors.lastname: Liu
authors.forename: Quan-hong
authors.initials: QH
authors.lastname: Ma
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism*; D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism*; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation / Q000502:physiology; D051379:Mice; D008822:Mice, Transgenic; D009474:Neurons / Q000378:metabolism*; D010446:Peptide Fragments / Q000378:metabolism*; D011485:Protein Binding
chemical_list: D016229:Amyloid beta-Peptides; D015816:Cell Adhesion Molecules, Neuronal; C491322:Cntnap1 protein, mouse; D010446:Peptide Fragments; C078592:amyloid beta-protein (1-40); C075222:amyloid beta-protein (1-42)
keywords: Alzheimer's disease; Amyloid Precursor Protein; Amyloid-β; Contactin associated protein; Neurodegenerative disease