title: N-acylhomoserine lactone-degrading bacteria isolated from hatchery bivalve larval cultures.
abstract: Quorum sensing (QS) systems, which depend on N-acylhomoserine lactone (AHL) signal molecules, mediate the production of virulence factors in many pathogenic microorganisms. One hundred and forty-six bacterial strains, isolated from a bivalve hatchery, were screened for their capacity to degrade five synthetic AHLs [N-butyryl-DL-homoserine lactone (C4-HSL), N-hexanoyl-DL-homoserine lactone (C6-HSL), N-octanoyl-DL-homoserine lactone (C8-HSL), N-decanoyl-DL-homoserine lactone (C10-HSL) and N-dodecanoyl-DL-homoserine lactone (C12-HSL)] using well diffusion agar-plate assays with three biosensors, Chromobacterium violaceum CV026, C. violaceum VIR07 and Agrobacterium tumefaciens NTL4 (pZLR4). The results of these assays led to our choosing four strains (PP2-67, PP2-459, PP2-644 and PP2-663) that were able to degrade all five synthetic AHLs, thus showing a wide spectrum of quorum quenching (QQ) activity. We subsequently confirmed and measured the QQ activity of the four strains by high-performance liquid chromatography plus mass-spectrometry analysis (HPLC-MS). One of the strains which showed the highest AHL-degrading activity, PP2-459, identified as being a member of the genus Thalassomonas was chosen for further study. Finally, using thin-layer chromatography (TLC), we went on to confirm this strain's capacity to degrade the AHLs produced by other non-pathogenic and pathogenic bacteria not taxonomically related.
authors.affiliation: Department of Microbiology, Faculty of Pharmacy, Cartuja Campus, University of Granada, 18071 Granada, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Torres
authors.forename: Manuel
authors.initials: M
authors.lastname: Romero
authors.forename: Susana
authors.initials: S
authors.lastname: Prado
authors.forename: Javier
authors.initials: J
authors.lastname: Dubert
authors.forename: Ali
authors.initials: A
authors.lastname: Tahrioui
authors.forename: Ana
authors.initials: A
authors.lastname: Otero
authors.forename: Inmaculada
authors.initials: I
authors.lastname: Llamas
mesh_terms: D054742:Acyl-Butyrolactones / Q000378:metabolism*; D000818:Animals; D001711:Biotransformation; D049872:Bivalvia / Q000254:growth & development / Q000382:microbiology*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D020563:Gammaproteobacteria / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism*; D007814:Larva / Q000382:microbiology; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D054742:Acyl-Butyrolactones; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
keywords: Aquaculture; Marine bacteria; Quorum quenching; Quorum sensing