title: Integrating DNA strand-displacement circuitry with DNA tile self-assembly.
abstract: DNA nanotechnology has emerged as a reliable and programmable way of controlling matter at the nanoscale through the specificity of Watson-Crick base pairing, allowing both complex self-assembled structures with nanometer precision and complex reaction networks implementing digital and analog behaviors. Here we show how two well-developed frameworks, DNA tile self-assembly and DNA strand-displacement circuits, can be systematically integrated to provide programmable kinetic control of self-assembly. We demonstrate the triggered and catalytic isothermal self-assembly of DNA nanotubes over 10 μm long from precursor DNA double-crossover tiles activated by an upstream DNA catalyst network. Integrating more sophisticated control circuits and tile systems could enable precise spatial and temporal organization of dynamic molecular structures.
authors.affiliation: Department of Computation and Neural Systems, California Institute of Technology, Pasadena, California, USA. dyz1@rice.edu
authors.forename: David Yu
authors.initials: DY
authors.lastname: Zhang
authors.forename: Rizal F
authors.initials: RF
authors.lastname: Hariadi
authors.forename: Harry M T
authors.initials: HM
authors.lastname: Choi
authors.forename: Erik
authors.initials: E
authors.lastname: Winfree
mesh_terms: D002384:Catalysis; D004247:DNA / Q000737:chemistry*; D007700:Kinetics; D008856:Microscopy, Fluorescence; D036103:Nanotechnology / Q000379:methods*; D043942:Nanotubes / Q000737:chemistry; D059008:Time-Lapse Imaging
chemical_list: D004247:DNA