title: Protective effect of silk lutein on ultraviolet B-irradiated human keratinocytes.
abstract: Carotenoids are efficient antioxidants that are of great importance for human health. Lutein and zeaxanthin are carotinoids present in high concentrations in the human retina which are involved in the photoprotection of the human eye. Lutein may also protect the skin from ultraviolet (UV)-induced damage. The present study investigated the protective effect of lutein extracted from yellow silk cocoons of Bombyx mori on human keratinocytes against UVB irradiation. A human keratinocyte cell line and primary human keratinocytes were used to investigate the UVB protection effects of silk lutein and plant lutein. Silk lutein showed no cytotoxicity to keratinocytes. Treatment with silk lutein prior to UVB irradiation enhanced cell viability and cell proliferation, and reduced cell apoptosis. The protective effects of silk lutein may be superior to those of plant lutein. Silk lutein may have a benefit for protection of keratinocytes against UVB-irradiation.
authors.affiliation: Department of Medicine, Faculty of Medicine, Naresuan University, Phitsanulok, Thailand. sutatipp@nu.ac.th
authors.forename: Sutatip
authors.initials: S
authors.lastname: Pongcharoen
authors.forename: Prateep
authors.initials: P
authors.lastname: Warnnissorn
authors.forename: Ongart
authors.initials: O
authors.lastname: Leŗtkajornsin
authors.forename: Nanteetip
authors.initials: N
authors.lastname: Limpeanchob
authors.forename: Manote
authors.initials: M
authors.lastname: Sutheerawattananonda
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D012831:Bombyx / Q000378:metabolism; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D016650:Fluorescein-5-isothiocyanate; D052816:Foreskin / Q000528:radiation effects; D006801:Humans; D015603:Keratinocytes / Q000528:radiation effects*; D014975:Lutein / Q000302:isolation & purification / Q000494:pharmacology*; D008297:Male; D061251:Primary Cell Culture; D011837:Radiation-Protective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D047011:Silk / Q000737:chemistry*; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D011837:Radiation-Protective Agents; D047011:Silk; D016650:Fluorescein-5-isothiocyanate; D014975:Lutein