title: Studying recombination with high-throughput sequencing: an educational primer for use with "fine-scale heterogeneity in crossover rate in the garnet-scalloped region of the Drosophila melanogaster X chromosome".
abstract: An article by Singh and colleagues in this issue of GENETICS quantifies variation in recombination rate across a small region of the Drosophila melanogaster genome, providing an opportunity for instructors of genetics to introduce or reinforce important concepts such as recombination and recombination rate variation, genome sequencing, and sequence features of the genome. Additional background information, a detailed explanation of the methods used in this study, and discussion questions are provided.
authors.affiliation: Biology Department, Duke University, Durham, NC 27708, USA.
authors.forename: Caiti S Smukowski
authors.initials: CS
authors.lastname: Heil
authors.forename: Mohamed A F
authors.initials: MA
authors.lastname: Noor
mesh_terms: D033963:Adaptor Protein Complex 3 / Q000235:genetics*; D033981:Adaptor Protein Complex delta Subunits / Q000235:genetics*; D000818:Animals; D059006:Chromosomes, Insect / Q000235:genetics*; D003434:Crossing Over, Genetic; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D005136:Eye Proteins / Q000235:genetics*; D018740:Genetic Heterogeneity; D014157:Transcription Factors / Q000235:genetics*; D014960:X Chromosome / Q000235:genetics*
chemical_list: D033963:Adaptor Protein Complex 3; D033981:Adaptor Protein Complex delta Subunits; D029721:Drosophila Proteins; D005136:Eye Proteins; C406291:G protein, Drosophila; D014157:Transcription Factors; C087984:sd protein, Drosophila
keywords: Drosophila melanogaster; crossing over; recombination