title: A systematic approach for the genetic dissection of protein complexes in living cells.
abstract: Cells contain many important protein complexes involved in performing and regulating structural, metabolic, and signaling functions. One major challenge in cell biology is to elucidate the organization and mechanisms of robustness of these complexes in vivo. We developed a systematic approach to study structural dependencies within complexes in living cells by deleting subunits and measuring pairwise interactions among other components. We used our methodology to perturb two conserved eukaryotic complexes: the retromer and the nuclear pore complex. Our results identify subunits that are critical for the assembly of these complexes, reveal their structural architecture, and uncover mechanisms by which protein interactions are modulated. Our results also show that paralogous proteins play a key role in the robustness of protein complexes and shape their assembly landscape. Our approach paves the way for studying the response of protein interactomes to mutations and enhances our understanding of genotype-phenotype maps.
authors.affiliation: Département de Biologie, PROTEO and Institut de Biologie Intégrative et des Systèmes, Université Laval, Québec, QC G1V 0A6, Canada.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Diss
authors.forename: Alexandre K
authors.initials: AK
authors.lastname: Dubé
authors.forename: Joël
authors.initials: J
authors.lastname: Boutin
authors.forename: Isabelle
authors.initials: I
authors.lastname: Gagnon-Arsenault
authors.forename: Christian R
authors.initials: CR
authors.lastname: Landry
mesh_terms: D002468:Cell Physiological Phenomena; D005838:Genotype; D008954:Models, Biological; D054730:Protein Interaction Domains and Motifs; D025941:Protein Interaction Mapping; D021122:Protein Subunits; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D021122:Protein Subunits; D011506:Proteins; D029701:Saccharomyces cerevisiae Proteins