title: A novel array format for monitoring cellular uptake using a photo-cleavable linker for peptide release.
abstract: We developed a novel peptide array format incorporating a photo-cleavable linker for monitoring cellular uptake. Model peptides were successfully immobilised via the photo-cleavable linker onto conventional plates and could be released spatiotemporally using UV irradiation. Incorporation of confocal microscopy allowed for detailed real-time monitoring of cellular internalisation of peptides.
authors.affiliation: Department of Bioengineering, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama 226-8501, Japan. kusui@center.konan-u.ac.jp
authors.forename: Kenji
authors.initials: K
authors.lastname: Usui
authors.forename: Takuya
authors.initials: T
authors.lastname: Kikuchi
authors.forename: Kin-ya
authors.initials: KY
authors.lastname: Tomizaki
authors.forename: Takashi
authors.initials: T
authors.lastname: Kakiyama
authors.forename: Hisakazu
authors.initials: H
authors.lastname: Mihara
mesh_terms: D001692:Biological Transport; D057846:Cell-Penetrating Peptides / Q000737:chemistry / Q000378:metabolism*; D003432:Cross-Linking Reagents / Q000737:chemistry / Q000528:radiation effects; D006367:HeLa Cells; D006801:Humans; D055767:Immobilized Proteins / Q000737:chemistry / Q000378:metabolism*; D055668:Photochemical Processes; D014466:Ultraviolet Rays
chemical_list: D057846:Cell-Penetrating Peptides; D003432:Cross-Linking Reagents; D055767:Immobilized Proteins