title: Diagnostic and prognostic implications of the PAX8-PPARγ translocation in thyroid carcinomas-a TMA-based study of 226 cases.
abstract: OBJECTIVE
  Follicular thyroid carcinoma (FTC) has been a diagnostic challenge for decades. The PAX8-PPARγ rearrangement has been detected in FTC and classic papillary thyroid carcinomas (PTCs). The aims of this study were to assess the presence of PAX8-PPARγ by using tissue microarrays in a large cohort of different thyroid neoplasms, and to assess its diagnostic and prognostic implications.
  
  
  RESULTS
  Fluorescence in-situ hybridization (FISH) analysis for PAX8-PPARγ was performed on 226 thyroid tumours, comprising FTCs (n = 59), PTCs (n = 126), poorly differentiated thyroid carcinomas (PDs; n = 34), follicular thyroid adenomas (FTAs; n = 5), and follicular tumours of unknown malignant potential (FTUMPs; n = 2). PAX8-PPARγ was detected in 12% of FTCs, 1% of PTCs, 7% of PDs, and in both cases of FTUMP. There was no correlation between the extent of capsular or vascular invasion and PAX8-PPARγ, or between lymph node or haematogenous metastasis and PAX8-PPARγ. Overall survival (OS), tumour-specific survival (TSS) and relapse-free-survival (RFS) were not influenced by PAX8-PPARγ.
  
  
  CONCLUSIONS
  In this study, we demonstrate for the first time the presence of PAX8-PPARγ in PDs and FTUMPs, whereas in FTCs and PTCs the prevalence of PAX8-PPARγ is lower than previously reported. PAX8-PPARγ did not correlate with invasiveness or affect prognosis in any tumour type.
authors.affiliation: Institute of Pathology, Technische Universität München, München, Germany.
authors.forename: Laura A
authors.initials: LA
authors.lastname: Boos
authors.forename: Matthias
authors.initials: M
authors.lastname: Dettmer
authors.forename: Anja
authors.initials: A
authors.lastname: Schmitt
authors.forename: Thomas
authors.initials: T
authors.lastname: Rudolph
authors.forename: Hans
authors.initials: H
authors.lastname: Steinert
authors.forename: Holger
authors.initials: H
authors.lastname: Moch
authors.forename: Manuel
authors.initials: M
authors.lastname: Sobrinho-Simões
authors.forename: Paul
authors.initials: P
authors.lastname: Komminoth
authors.forename: Aurel
authors.initials: A
authors.lastname: Perren
mesh_terms: D018263:Adenocarcinoma, Follicular / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism; D000236:Adenoma / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism; D002277:Carcinoma / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism; D002291:Carcinoma, Papillary; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis / Q000235:genetics / Q000473:pathology; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D015514:Oncogene Proteins, Fusion / Q000235:genetics* / Q000378:metabolism; D000072636:PAX8 Transcription Factor; D047495:PPAR gamma / Q000235:genetics*; D051761:Paired Box Transcription Factors / Q000235:genetics*; D011379:Prognosis; D012189:Retrospective Studies; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000175:diagnosis* / Q000235:genetics* / Q000378:metabolism; D046888:Tissue Array Analysis; D014178:Translocation, Genetic
chemical_list: D015514:Oncogene Proteins, Fusion; D000072636:PAX8 Transcription Factor; C083325:PAX8 protein, human; C551995:PAX8-PPARgamma fusion protein, human; D047495:PPAR gamma; D051761:Paired Box Transcription Factors
keywords: FISH; PAX8-PPARγ; angioinvasion; outcome; prognosis; thyroid carcinoma