title: Protein expressions and their immunogenicity from Riemerella anatipestifer cultured in iron restriction medium.
abstract: Riemerella anatipestifer was cultured in both iron restriction media and normal media. Two-dimensional gel electrophoresis identified 23 proteins that significantly increased in the iron restriction media. Of them 12 proteins were analyzed with mass spectrography. Nine of 12 proteins belong to 6 different protein families: fibronectin type iii domain protein, secreted subtilase family protein, phosphoglycerate kinase, translation elongation factor, leucine-rich repeat-containing protein, and Galactose-binding domain-like protein. Other 3 proteins were novel with unknown function. Two novel proteins (Riean_1750 and Riean_1752) were expressed in prokaryotic expression systems. The specificities of these 2 novel proteins to R. anatipestifer were confirmed by western-blotting analysis. The ducks immunized with either protein had low mortality challenged by R. anatipestifer, 33.3% and 16.7%, respectively. The ducks developed 100% immunity when immunized with combined Riean_1750 and Riean_1752 proteins. The data suggested 2 novel proteins play important roles in the bacterial survival in the iron restricted environment. They could be used as subunit vaccines of R. anatipestifer.
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
authors.forename: Yifei
authors.initials: Y
authors.lastname: Yang
authors.forename: Changqin
authors.initials: C
authors.lastname: Gu
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Liao
authors.forename: Qingping
authors.initials: Q
authors.lastname: Luo
authors.forename: Xueying
authors.initials: X
authors.lastname: Hu
authors.forename: Wanpo
authors.initials: W
authors.lastname: Zhang
authors.forename: Huabin
authors.initials: H
authors.lastname: Shao
authors.forename: Guofu
authors.initials: G
authors.lastname: Cheng
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000276:immunology; D003470:Culture Media / Q000737:chemistry; D004372:Ducks; D005353:Fibronectins / Q000235:genetics / Q000276:immunology; D045826:Flavobacteriaceae Infections / Q000276:immunology / Q000401:mortality / Q000517:prevention & control / Q000662:veterinary*; D015870:Gene Expression; D007114:Immunization; D000090463:Iron Deficiencies; D009004:Monosaccharide Transport Proteins / Q000235:genetics / Q000276:immunology; D010445:Peptide Elongation Factors / Q000235:genetics / Q000276:immunology; D033902:Periplasmic Binding Proteins / Q000235:genetics / Q000276:immunology; D010735:Phosphoglycerate Kinase / Q000235:genetics / Q000276:immunology; D011201:Poultry Diseases / Q000276:immunology / Q000401:mortality / Q000517:prevention & control* / Q000821:virology; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D060494:Riemerella / Q000254:growth & development / Q000276:immunology* / Q000378:metabolism; D013381:Subtilisins / Q000235:genetics / Q000276:immunology; D016019:Survival Analysis
chemical_list: D001426:Bacterial Proteins; D002135:Calcium-Binding Proteins; D003470:Culture Media; D005353:Fibronectins; D009004:Monosaccharide Transport Proteins; D010445:Peptide Elongation Factors; D033902:Periplasmic Binding Proteins; D011994:Recombinant Proteins; C023550:galactose-binding protein; D010735:Phosphoglycerate Kinase; D013381:Subtilisins