title: [The effect of nicotinamide N-methytransferase overexpression on biological behaviors of SMMC7721 hepatocellar carcinoma cell line].
abstract: OBJECTIVE
  To study the effect of over expressed nicotinamide N-methytransferase (NNMT) on the malignant biological behaviors of hepatocellular carcinoma cell line SMMC7721.
  
  
  METHODS
  The cells transfected with SMMC/NNMT abbreviated as S/NNMT, parental cells including the negative control which transfected with SMMC/pcDNA3. 1 abbreviated as S/P and parental group which abbreviated S. MTT assay and Plate Clone Formation Assay, cell adhension assay and invasion assay were applied to evaluate the effect of NNMT overexpression on the malignant biological behaviors of hepatocellular carcinoma cell line SMMC7721.
  
  
  RESULTS
  Compared with the negative control and parental group, MTT assay and Plate Clone Formation Assay demonstrated that over expression of NNMT in SMMC7721 cells could not influence the proliferation clone formation of SMMC7721 cells (P > 0.05). Over expression of NNMT in SMMC7721 cells significantly enhanced the ability of invasion and adhension (P < 0.001).
  
  
  CONCLUSIONS
  NNMT gene can partially influence the malignant behaviors of hepatocellular carcinoma cellline SMMC7721 and increased the ability of invasion and adhension.
authors.affiliation: Department of Infection Control, Guizhou Provincial People's Hospital, Guiyang 550002, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Mu
authors.forename: Yao
authors.initials: Y
authors.lastname: Chen
authors.forename: Sui-Hai
authors.initials: SH
authors.lastname: Wang
authors.forename: Ming
authors.initials: M
authors.lastname: Li
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000201:enzymology* / Q000473:pathology; D006801:Humans; D008113:Liver Neoplasms / Q000201:enzymology* / Q000473:pathology*; D009361:Neoplasm Invasiveness; D050903:Nicotinamide N-Methyltransferase / Q000235:genetics / Q000378:metabolism*; D014162:Transfection; D014407:Tumor Cells, Cultured
chemical_list: C494406:NNMT protein, human; D050903:Nicotinamide N-Methyltransferase