title: New advances in countercurrent chromatography and centrifugal partition chromatography: focus on coupling strategy.
abstract: Countercurrent chromatography (CCC) is an attractive separation method because the analytes are partitioned between two immiscible liquid phases avoiding problems related to solid stationary phase. In recent years, this technique has made great progress in separation power and detection potential. This review describes coupling strategies involving high speed CCC (HSCCC) or centrifugal partition chromatography (CPC). It includes on-line extraction-isolation, hyphenation with mass spectrometry (MS) and nuclear magnetic resonance (NMR) detectors, multidimensional CCC (MDCCC), two-dimensional CCC (2D-CCC), on-line coupling with liquid chromatography (LC), and biological tests, and innovative off-line developments. The basic principles of each method are presented and applications are summarized.
authors.affiliation: Université d'Orléans-CNRS, Institut de Chimie Organique et Analytique UMR 7311, BP 6759 rue de Chartres, 45067, Orléans cedex 2, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Michel
authors.forename: Emilie
authors.initials: E
authors.lastname: Destandau
authors.forename: Claire
authors.initials: C
authors.lastname: Elfakir
mesh_terms: D000818:Animals; D002498:Centrifugation / Q000295:instrumentation / Q000379:methods / Q000639:trends*; D016500:Color Therapy / Q000295:instrumentation / Q000379:methods / Q000639:trends; D003377:Countercurrent Distribution / Q000295:instrumentation / Q000379:methods / Q000639:trends*; D006801:Humans; D010455:Peptides / Q000032:analysis / Q000302:isolation & purification; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification
chemical_list: D010455:Peptides; D010936:Plant Extracts