title: Transcriptional control of the main aromatic hydrocarbon efflux pump in Pseudomonas.
abstract: Bacteria of the species Pseudomonas putida are ubiquitous soil inhabitants, and a few strains are able to thrive in the presence of extremely high concentrations of toxic solvents such as toluene and related aromatic hydrocarbons. Toluene tolerance is multifactorial in the sense that bacteria use a wide range of physiological and genetic changes to overcome solvent damage. This includes enhanced membrane impermeabilization through cis to trans isomerization of unsaturated fatty acids, activation of a stress response programme, and induction of efflux pumps that expulse toxic hydrocarbons to the outer medium. The most relevant element in this toluene tolerance arsenal is the TtgGHI efflux pump controlled by the TtgV regulator. We discuss here how TtgV controls expression of this efflux pump in response to solvents.
authors.affiliation: Consejo Superior de Investigaciones Científicas, EEZ, Department of Environmental Protection, Granada, Spain. University of Toronto, Department of Banting and Best Department of Medical Research, Toronto, Canada. Imperial College of Science and Technology, London, UK.
authors.forename: Sandy
authors.initials: S
authors.lastname: Fillet
authors.forename: Craig
authors.initials: C
authors.lastname: Daniels
authors.forename: Cecilia
authors.initials: C
authors.lastname: Pini
authors.forename: Tino
authors.initials: T
authors.lastname: Krell
authors.forename: Estrella
authors.initials: E
authors.lastname: Duque
authors.forename: Patricia
authors.initials: P
authors.lastname: Bernal
authors.forename: Ana
authors.initials: A
authors.lastname: Segura
authors.forename: Duo
authors.initials: D
authors.lastname: Lu
authors.forename: Xiadong
authors.initials: X
authors.lastname: Zhang
authors.forename: Juan-Luis
authors.initials: JL
authors.lastname: Ramos