title: Immune response to acetaldehyde-human serum albumin adduct among healthy subjects related to alcohol intake.
abstract: Acetaldehyde (AA) is the main metabolic product in ethanol metabolism, although it can also derive from sources of airborne pollution. As a typical aldehyde, AA is able to react with a variety of molecular targets, including DNA and protein. This property justifies the hypothesis of a immune reaction against this kind of adduct, to be studied by a seroprevalence screening approach. In this study, the correlation between drinking habits and the amount of circulating AA-human serum albumin adduct (AA-HSA) was evaluated in a group of healthy subjects, non alcohol-addicted. Daily ethanol intake (grams) was inferred for each subject using the information collected through a questionnaire, and AA-HSA antibodies (AA-HSA ab) analyses were performed using the Displacement Assay on whole blood samples. The findings showed a correlation between ethanol intake and immune response to molecular adduct. These results underscore the evaluation of AA-HSA ab amount as a suitable molecular marker for alcohol intake that can be applied in future investigations on a large scale for prevention screening.
authors.affiliation: Department of Public Health and Pediatrics, University of Torino, Via Santena 5 bis, Torino, 10126, Italy. Electronic address: valeria.romanazzi@unito.it.
authors.forename: Valeria
authors.initials: V
authors.lastname: Romanazzi
authors.affiliation: Department of Public Health and Pediatrics, University of Torino, Via Santena 5 bis, Torino, 10126, Italy. Electronic address: tiziana.schiliro@unito.it.
authors.forename: Tiziana
authors.initials: T
authors.lastname: Schilirò
authors.affiliation: Department of Public Health and Pediatrics, University of Torino, Via Santena 5 bis, Torino, 10126, Italy. Electronic address: elisabetta.carraro@unito.it.
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Carraro
authors.affiliation: Department of Public Health and Pediatrics, University of Torino, Via Santena 5 bis, Torino, 10126, Italy. Electronic address: giorgio.gilli@unito.it.
authors.forename: Giorgio
authors.initials: G
authors.lastname: Gilli
mesh_terms: D000079:Acetaldehyde / Q000097:blood / Q000276:immunology*; D000328:Adult; D000428:Alcohol Drinking / Q000097:blood / Q000276:immunology*; D000704:Analysis of Variance; D001323:Autoantibodies / Q000097:blood*; D015415:Biomarkers / Q000097:blood; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D008297:Male; D008875:Middle Aged; D016036:Seroepidemiologic Studies; D012709:Serum Albumin / Q000276:immunology*; D000075462:Serum Albumin, Human; D011795:Surveys and Questionnaires
chemical_list: C422455:ALB protein, human; D001323:Autoantibodies; D015415:Biomarkers; D012709:Serum Albumin; D000079:Acetaldehyde; D000075462:Serum Albumin, Human
keywords: AA; AA-HSA ab; AA-HSA antibodies; Acetaldehyde; Ethanol; HSA; Immune response; Molecular adduct; acetaldehyde; human serum albumin