title: Chondrogenic potential of human dermal fibroblasts in a contractile, soft, self-assembling, peptide hydrogel.
abstract: The present paper describes a simple approach to obtain three-dimensional (3D) cartilage constructs using human normal dermal fibroblasts (hNDFs) cultured in a self-assembling peptide nanofibre scaffold. During the first days of culture, the 3D constructs underwent morphological changes consisting of a substantial contraction process that ended in a small compact structure. During this process the system became sensitive to induction with standard chondrogenic medium, evidenced by the expression of specific markers of mature cartilage. First, it was detected that the samples become highly stained with toluidine blue dye over time (40-50 days), indicating that the system produced significantly high amounts of glycosaminoglycans. By quantitative PCR, it was confirmed that the system significantly upregulated the expression of the proteoglycan aggrecan, a good indicator of cartilage commitment. Moreover, collagen type II was upregulated at protein level, confirming that the system differentiated to a chondrocyte-like construct. Additionally, during the first days of culture in control medium analysed hNDFs proliferation capacity in this 3D system was analysed. This platform could be used in the future to obtain an autologous source of cells from a simple patient skin biopsy, which could be easily translated into a low-cost and effective regenerative therapy.
authors.affiliation: Translational Centre for Regenerative Medicine (TRM-Leipzig), Universität Leipzig, Germany.
authors.forename: Bianca M
authors.initials: BM
authors.lastname: Bussmann
authors.affiliation: Translational Centre for Regenerative Medicine (TRM-Leipzig), Universität Leipzig, Germany.
authors.forename: Sven
authors.initials: S
authors.lastname: Reiche
authors.affiliation: Department of Bioengineering, Tissue Engineering Laboratory, Institut Químic de Sarrià -Universitat Ramon Llull, Barcelona, Spain.
authors.forename: Núria
authors.initials: N
authors.lastname: Marí-Buyé
authors.affiliation: Department of Bioengineering, Tissue Engineering Laboratory, Institut Químic de Sarrià -Universitat Ramon Llull, Barcelona, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Castells-Sala
authors.affiliation: Department of Neurosurgery, BG Hospital Bergmannstrost, Halle, Germany.
authors.forename: Hans Jörg
authors.initials: HJ
authors.lastname: Meisel
authors.affiliation: Translational Centre for Regenerative Medicine (TRM-Leipzig), Universität Leipzig, Germany.|Department of Bioengineering, Tissue Engineering Laboratory, Institut Químic de Sarrià -Universitat Ramon Llull, Barcelona, Spain.
authors.forename: Carlos E
authors.initials: CE
authors.lastname: Semino
mesh_terms: D001205:Ascorbic Acid / Q000494:pharmacology; D015415:Biomarkers / Q000378:metabolism; D002113:Calcification, Physiologic / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D020219:Chondrogenesis / Q000187:drug effects*; D024043:Collagen Type II / Q000378:metabolism; D020405:Dermis / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects; D006025:Glycosaminoglycans / Q000378:metabolism; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000494:pharmacology*; D010455:Peptides / Q000494:pharmacology*; D053782:Transforming Growth Factor beta3 / Q000494:pharmacology
chemical_list: D015415:Biomarkers; D024043:Collagen Type II; D006025:Glycosaminoglycans; D010455:Peptides; D053782:Transforming Growth Factor beta3; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D001205:Ascorbic Acid
keywords: chondrogenesis; dermal fibroblasts; in vitro; self-assembling peptide; three-dimensional; tissue engineering