title: Microarray comparison of anterior and posterior Drosophila wing imaginal disc cells identifies novel wing genes.
abstract: Signaling between cells in the anterior (A) and posterior (P) compartments directs Drosophila wing disc development and is dependent on expression of the homeodomain transcription factor Engrailed (En) in P cells. Downstream of en, posteriorly expressed Hedgehog (Hh) protein signals across the A/P border to establish a developmental organizer that directs pattern formation and growth throughout the wing primordium. Here we extend investigations of the processes downstream of en by using expression array analysis to compare A and P cells. A total of 102 candidate genes were identified that express differentially in the A and P compartments; four were characterized: Stubble (Sb) expression is restricted to A cells due to repression by en. CG15905, CG16884; CG10200/hase und igel (hui) are expressed in A cells downstream of Hh signaling; and RNA interference for hui, Stubble, and CG16884 revealed that each is essential to wing development.
authors.affiliation: Freie Universität Berlin, Institut für Biologie, Genetik, 14195 Berlin, Germany.
authors.forename: Daniel M
authors.initials: DM
authors.lastname: Ibrahim
authors.forename: Brian
authors.initials: B
authors.lastname: Biehs
authors.forename: Thomas B
authors.initials: TB
authors.lastname: Kornberg
authors.forename: Ansgar
authors.initials: A
authors.lastname: Klebes
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D004330:Drosophila / Q000235:genetics* / Q000254:growth & development; D029721:Drosophila Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D053823:Hedgehog Proteins / Q000235:genetics / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D060227:Imaginal Discs / Q000166:cytology* / Q000378:metabolism; D008565:Membrane Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D034622:RNA Interference; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014921:Wings, Animal / Q000166:cytology*
chemical_list: D029721:Drosophila Proteins; C063459:En protein, Drosophila; D053823:Hedgehog Proteins; D018398:Homeodomain Proteins; D008565:Membrane Proteins; D014157:Transcription Factors; C077245:hh protein, Drosophila; D012697:Serine Endopeptidases; C081525:sb protein, Drosophila
keywords: anteroposterior compartment border; engrailed; expression microarray; hedgehog; pattern formation