title: Inhibition of multiplication of the prototypic arenavirus LCMV by valproic acid.
abstract: Valproic acid (VPA), a short chain fatty acid commonly used for treatment of neurological disorders, has been shown to inhibit production of infectious progeny of different enveloped viruses including the prototypic arenavirus lymphocytic choriomeningitis virus (LCMV). In this study we have investigated the mechanisms by which VPA inhibits LCMV multiplication in cultured cells. VPA reduced production of infectious LCMV progeny and virus propagation without exerting a major blockage on either viral RNA or protein synthesis, but rather affecting the cell release and specific infectivity of LCMV progeny from infected cells. Our results would support the repurposing of VPA as a candidate antiviral drug to combat arenavirus infections.
authors.affiliation: Centro de Biología Molecular Severo Ochoa UAM-CSIC, Cantoblanco, Madrid, Spain.
authors.forename: Ángela
authors.initials: Á
authors.lastname: Vázquez-Calvo
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Martín-Acebes
authors.forename: Juan-Carlos
authors.initials: JC
authors.lastname: Sáiz
authors.forename: Nhi
authors.initials: N
authors.lastname: Ngo
authors.forename: Francisco
authors.initials: F
authors.lastname: Sobrino
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: de la Torre
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D001117:Arenaviridae Infections / Q000188:drug therapy / Q000821:virology; D002460:Cell Line; D002522:Chlorocebus aethiops; D006224:Cricetinae; D055808:Drug Discovery; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D057809:HEK293 Cells; D006801:Humans; D008217:Lymphocytic choriomeningitis virus / Q000187:drug effects* / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology; D012367:RNA, Viral / Q000096:biosynthesis; D014635:Valproic Acid / Q000494:pharmacology*; D014709:Vero Cells; D057074:Virus Release / Q000187:drug effects*; D014779:Virus Replication / Q000187:drug effects*
chemical_list: D000998:Antiviral Agents; D004791:Enzyme Inhibitors; D012367:RNA, Viral; D014635:Valproic Acid
keywords: Antiviral; Budding; LCMV; Valproic acid