title: Electron capture dissociation and collision induced dissociation of S-dipalmitoylated peptides.
abstract: Here we investigate the effect of S-dipalmitoylation on the electron capture dissociation (ECD) behavior of peptides. The ECD and collision induced dissociation (CID) of peptides modified by covalent attachment of [(RS)-2,3-di(palmitoyloxy)-propyl] (PAM2) group to cysteine residues [C(PAM2)LEYDTGFK and RPPGC(PAM2)SPFK] were examined. The results suggest that ECD of S-dipalmitoylated peptides can provide both primary sequence information and structural information regarding the modification. The structural information provided by CID is complementary to that provided by ECD.
authors.affiliation: Faculty of Chemical Technology and Engineering, University of Technology and Life Sciences in Bydgoszcz, Seminaryjna 3, Bydgoszcz, Poland. Malgorzata.Kaczorowska@utp.edu.pl
authors.forename: Małgorzata A
authors.initials: MA
authors.lastname: Kaczorowska
authors.forename: Helen J
authors.initials: HJ
authors.lastname: Cooper
mesh_terms: D000595:Amino Acid Sequence; D003545:Cysteine / Q000737:chemistry; D004578:Electron Spin Resonance Spectroscopy; D007202:Indicators and Reagents; D010169:Palmitic Acids / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D007202:Indicators and Reagents; D010169:Palmitic Acids; D010455:Peptides; D003545:Cysteine