title: In vivo monitoring of survival and proliferation of hair stem cells in a hair follicle generation animal model.
abstract: The purpose of this study was to investigate in vivo monitoring of hair follicle stem cells (newborn mouse fibroblasts [NFs]) expressing enhanced firefly luciferase (effluc) (NF-effluc) using noninvasive bioluminescence imaging (BLI). Effluc gene transduction into NFs was performed by retroviral vector, and effluc messenger ribonucleic acid expression and function were evaluated by reverse transcription-polymerase chain reaction and luciferase assay. After in vivo transplantation of NF-effluc cells to generate hair follicles, BLI was performed on days 1, 3, 7, 14, and 21 after transplantation. Additionally, hair follicle generation by the implanted stem cells was investigated using microscopy. The luciferase activity of NF-effluc was 41,175-fold higher compared to that of untransfected NFs. Bioluminescence signals from the transplantation site decreased gradually over 2 weeks; then the signal plateaued. Hair follicles were confirmed at the NF-effluc cell implantation site on day 14 after transplantation. We successfully monitored hair generation by hair stem cell implantation noninvasively with optical molecular strategy in an in vivo model.
authors.affiliation: Departments of Nuclear Medicine and Immunology, Kyungpook National University School of Medicine, Daegu, South Korea, 700-721.
authors.forename: Jung
authors.initials: J
authors.lastname: Eun Kim
authors.forename: Byeong-Cheol
authors.initials: BC
authors.lastname: Ahn
authors.forename: Ho
authors.initials: H
authors.lastname: Won Lee
authors.forename: Mi-Hye
authors.initials: MH
authors.lastname: Hwang
authors.forename: Seung
authors.initials: S
authors.lastname: Hyun Shin
authors.forename: Sang
authors.initials: S
authors.lastname: Woo Lee
authors.forename: Young
authors.initials: Y
authors.lastname: Kwan Sung
authors.forename: Jaetae
authors.initials: J
authors.lastname: Lee
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D049109:Cell Proliferation; D002470:Cell Survival; D057075:Enzyme Assays; D005347:Fibroblasts / Q000166:cytology; D018859:Hair Follicle / Q000166:cytology*; D008156:Luciferases / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008819:Mice, Nude; D023421:Models, Animal; D057054:Molecular Imaging; D013234:Stem Cells / Q000166:cytology*
chemical_list: D008156:Luciferases