title: The effects of HNE on ovine oxymyoglobin redox stability in a microsome model.
abstract: The effect of 4-hydroxy-2-nonenal (HNE), a secondary lipid oxidation product, on ovine myoglobin (Mb) redox stability was investigated. HNE increased oxymyoglobin (OxyMb) oxidation under all pH/temperature conditions studied. Mono-, di- and tri-HNE adducts were detected by ESI-Q-TOF MS analysis. Sites of adduction, His 120, His 25 and His 65, were determined by ESI-CID-MS/MS analysis. The relationship between ovine Mb (with/without HNE) and lipid oxidation was also studied in a microsome model in the presence of α-tocopherol. Surprisingly, preincubation of Mb with HNE did not affect subsequent Mb redox stability in the microsome model (P<0.05). Microsomes with elevated concentrations of α-tocopherol delayed lipid and Mb oxidations relative to controls. HNE-treated ovine Mb caused greater lipid oxidation compared to control ovine Mb in control microsomes (P<0.05). This study demonstrated an interaction between ovine Mb oxidation and lipid oxidation.
authors.affiliation: Department of Food Science, Pennsylvania State University, State College, PA, USA.
authors.forename: Shuang
authors.initials: S
authors.lastname: Yin
authors.forename: Cameron
authors.initials: C
authors.lastname: Faustman
authors.forename: Nantawat
authors.initials: N
authors.lastname: Tatiyaborworntham
authors.forename: Ranjith
authors.initials: R
authors.lastname: Ramanathan
authors.forename: Qun
authors.initials: Q
authors.lastname: Sun
mesh_terms: D000447:Aldehydes / Q000009:adverse effects*; D000818:Animals; D002853:Chromatography, Liquid; D006639:Histidine / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D050356:Lipid Metabolism / Q000187:drug effects; D008861:Microsomes / Q000187:drug effects / Q000378:metabolism; D009211:Myoglobin / Q000737:chemistry*; D010084:Oxidation-Reduction / Q000187:drug effects*; D034561:Sheep, Domestic; D053719:Tandem Mass Spectrometry; D013696:Temperature; D024502:alpha-Tocopherol / Q000494:pharmacology
chemical_list: D000447:Aldehydes; D009211:Myoglobin; C017595:oxymyoglobin; D006639:Histidine; D024502:alpha-Tocopherol; C027576:4-hydroxy-2-nonenal