title: Klebsiella pneumoniae sequence type 11 from companion animals bearing ArmA methyltransferase, DHA-1 β-lactamase, and QnrB4.
abstract: Seven Klebsiella pneumoniae isolates from dogs and cats in Spain were found to be highly resistant to aminoglycosides, and ArmA methyltransferase was responsible for this phenotype. All isolates were typed by multilocus sequence typing (MLST) as ST11, a human epidemic clone reported worldwide and associated with, among others, OXA-48 and NDM carbapenemases. In the seven strains, armA was borne by an IncR plasmid, pB1025, of 50 kb. The isolates were found to coproduce DHA-1 and SHV-11 β-lactamases, as well as the QnrB4 resistance determinant. This first report of the ArmA methyltransferase in pets illustrates their importance as a reservoir for human multidrug-resistant K. pneumoniae.
authors.affiliation: Department of Animal Health and VISAVET, Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Hidalgo
authors.forename: Belen
authors.initials: B
authors.lastname: Gutierrez
authors.forename: Cristina M
authors.initials: CM
authors.lastname: Ovejero
authors.forename: Laura
authors.initials: L
authors.lastname: Carrilero
authors.forename: Stephanie
authors.initials: S
authors.lastname: Matrat
authors.forename: Courage K S
authors.initials: CK
authors.lastname: Saba
authors.forename: Alfonso
authors.initials: A
authors.lastname: Santos-Lopez
authors.forename: Daniel
authors.initials: D
authors.lastname: Thomas-Lopez
authors.forename: Andreas
authors.initials: A
authors.lastname: Hoefer
authors.forename: Monica
authors.initials: M
authors.lastname: Suarez
authors.forename: Gloria
authors.initials: G
authors.lastname: Santurde
authors.forename: Carmen
authors.initials: C
authors.lastname: Martin-Espada
authors.forename: Bruno
authors.initials: B
authors.lastname: Gonzalez-Zorn
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D002371:Cat Diseases / Q000453:epidemiology / Q000382:microbiology*; D002415:Cats; D004283:Dog Diseases / Q000453:epidemiology / Q000382:microbiology*; D004285:Dogs; D006801:Humans; D007710:Klebsiella Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000235:genetics; D008780:Methyltransferases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D058885:Multilocus Sequence Typing; D010957:Plasmids / Q000737:chemistry; D020033:Protein Isoforms / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013030:Spain / Q000453:epidemiology; D001618:beta-Lactamases / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D020033:Protein Isoforms; C485385:Qnr protein, Klebsiella pneumoniae; D008780:Methyltransferases; D001618:beta-Lactamases