title: Heterotopic and orthotopic autologous chondrocyte implantation using a minipig chondral defect model.
abstract: Implantation of non-articular (heterotopic) chondrocyte-based implants might be an alternative approach to articular cartilage repair. This strategy could be helpful in cases in which there are no or too few articular chondrocytes available. Therefore, this study was undertaken to compare joint cartilage defect healing in the minipig model after implantation of heterotopic auricular and orthotopic articular chondrocytes. Poly-glycolic acid (PGA) associated three-dimensional (3D) constructs were prepared culturing autologous minipig-derived articular and auricular chondrocytes for 7 days in a dynamic culture system. Chondrocyte PGA constructs were implanted into 8mm diameter and ∼1.1mm deep chondral defects within the medial and lateral condyles of the minipig knee joints. Empty defects served as controls for assessment of the intrinsic healing response. Defect healing was monitored 6 months post implantation using a macroscopic and microscopic score system and biomechanical analysis. Neo-cartilage formation could be observed in the PGA constructs seeded with articular and auricular chondrocytes in vivo. The defect healing did not significantly differ at the macroscopic and histological level in response to implantation of either autologous articular or auricular chondrocytes seeded constructs compared with the empty defects. Although the differences were not significant, the auricular chondrocytes-based implants led to a slightly inferior repair quality at the macroscopic level, but a histologically superior healing response when compared with the empty defect group. However, biomechanical analysis revealed a higher stiffness in repair tissues produced by auricular chondrocyte implantation compared with the other groups. Deduced from these results, articular chondrocytes represent the preferable cell source for implantation.
authors.affiliation: Department for Orthopaedic, Trauma and Reconstructive Surgery, Charité-University of Medicine, Campus Benjamin Franklin, Berlin, Germany.
authors.forename: Anke
authors.initials: A
authors.lastname: Lohan
authors.forename: Ulrike
authors.initials: U
authors.lastname: Marzahn
authors.forename: Karym
authors.initials: K
authors.lastname: El Sayed
authors.forename: Christopher
authors.initials: C
authors.lastname: Bock
authors.forename: Andreas
authors.initials: A
authors.lastname: Haisch
authors.forename: Benjamin
authors.initials: B
authors.lastname: Kohl
authors.forename: Katharina
authors.initials: K
authors.lastname: Stoelzel
authors.forename: Thilo
authors.initials: T
authors.lastname: John
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Ertel
authors.forename: Gundula
authors.initials: G
authors.lastname: Schulze-Tanzil
mesh_terms: D000818:Animals; D001168:Arthritis / Q000473:pathology; D001696:Biomechanical Phenomena; D001706:Biopsy; D002357:Cartilage Diseases / Q000503:physiopathology / Q000601:surgery*; D002358:Cartilage, Articular / Q000166:cytology*; D017690:Cell Transplantation / Q000379:methods*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000637:transplantation*; D004396:Coloring Agents; D005684:Gait; D007150:Immunohistochemistry; D007596:Joints / Q000473:pathology; D011100:Polyglycolic Acid / Q000494:pharmacology; D011187:Posture / Q000502:physiology; D013552:Swine; D013556:Swine, Miniature; D016042:Transplantation, Heterotopic / Q000379:methods*
chemical_list: D004396:Coloring Agents; D011100:Polyglycolic Acid
keywords: Articular; Auricular; Chondral defect; Chondrocytes; Joint cartilage; Minipig; Repair