title: Use of the Ussing chamber technique to study nutrient transport by epithelial tissues.
abstract: The Ussing chamber provides a physiologically relevant system for measuring the transport of ions, nutrients, and drugs across various epithelial tissues. This article outlines the design, structure, principle, and operation of the Ussing chamber, its application in the field of gastrointestinal barrier function and nutrient transport research, as well as its advantages and limitations. This review serves as a practical guide for investigators who are new to the Ussing chamber and should help researchers better understand this valuable method for measuring the transport of electrolytes, organic nutrients, water, and drugs across the small intestine, placenta, and other epithelial tissues.
authors.affiliation: Chinese Academy of Sciences, Institute of Subtropical Agriculture, Research Center for Healthy Breeding Livestock and Poultry, Changsha, Hunan, China.
authors.forename: Liuqin
authors.initials: L
authors.lastname: He
authors.forename: Yulong
authors.initials: Y
authors.lastname: Yin
authors.forename: Tiejun
authors.initials: T
authors.lastname: Li
authors.forename: Rulin
authors.initials: R
authors.lastname: Huang
authors.forename: Mingyong
authors.initials: M
authors.lastname: Xie
authors.forename: Zhenlong
authors.initials: Z
authors.lastname: Wu
authors.forename: Guoyao
authors.initials: G
authors.lastname: Wu
mesh_terms: D000818:Animals; D001692:Biological Transport; D004848:Epithelium / Q000378:metabolism; D004864:Equipment and Supplies; D005502:Food; D007413:Intestinal Mucosa / Q000378:metabolism*