title: Cloning, characterization, and promoter analysis of mouse Myo10 gene.
abstract: Myosin X (Myo10) is an unconventional myosin associated with filopodia motility. Recent studies show that in addition to full-length Myo10, brain expresses a shorter form of Myo10 that lacks a myosin motor domain named headless Myo10. Herein, we analyzed and cloned 2-kb of the 5'-upstream sequences of mouse full-length Myo10 (fMyo10) and headless Myo10 (hMyo10) to understand the transcriptional regulation of the Myo10 gene. The putative transcription factor binding sites and CpG island were analyzed by a bioinformatic approach. Luciferase reporter assays showed that the 2-kb of 5'-upstream sequences of both fMyo10 and hMyo10 had promoter activities.
authors.affiliation: Key Laboratory of Molecular Epigenetics of Ministry of Education, Institute of Cytology and Genetics, Northeast Normal University, Changchun, China.
authors.forename: Mingming
authors.initials: M
authors.lastname: Lai
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: Xingda
authors.initials: X
authors.lastname: Ju
authors.forename: Huali
authors.initials: H
authors.lastname: Yu
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018899:CpG Islands; D051379:Mice; D009218:Myosins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D015533:Transcriptional Activation
chemical_list: C409064:Myo10 protein, mouse; D009218:Myosins