title: The role of PCR in diagnostics of Lyme borreliosis.
abstract: Molecular biology techniques play a significant role in diagnostics of many infectious diseases. Polymerase chain reaction (PCR) is used to confirm tick-borne diseases e.g. Borrelia burgdorferi infection. Extension of PCR method in laboratory diagnostics of Lyme disease (LD) gives a possibility of confirmation of spirochete infection before patient body managed to produce antibodies. Diversity of material, which may be tested (blood, cerebrospinal fluid, skin biopsies, synovial fluid) and possibility of using several conservative genes for Borrelia genospecies additionally widen a diagnostic utility of PCR tests. Though high divergence of PCR results obtained in various laboratories is still a significant issue. Hence, standardization of molecular diagnostic in LD is so important.
authors.affiliation: Department of Neuroinfections and Infectious Diseases, Medical University of Bialystok, Poland. neuroin@umwb.edu.pl
authors.forename: Justyna
authors.initials: J
authors.lastname: Dunaj
authors.forename: Anna
authors.initials: A
authors.lastname: Moniuszko
authors.forename: Joanna
authors.initials: J
authors.lastname: Zajkowska
authors.forename: Sławomir
authors.initials: S
authors.lastname: Pancewicz
mesh_terms: D000818:Animals; D015748:Borrelia burgdorferi Group / Q000235:genetics / Q000302:isolation & purification*; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000235:genetics / Q000302:isolation & purification; D005819:Genetic Markers; D006801:Humans; D018884:Ixodes / Q000382:microbiology; D008193:Lyme Disease / Q000175:diagnosis* / Q000382:microbiology*; D011044:Poland / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012338:RNA, Ribosomal, 23S / Q000235:genetics; D012341:RNA, Ribosomal, 5S / Q000235:genetics; D012680:Sensitivity and Specificity
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005819:Genetic Markers; D012336:RNA, Ribosomal, 16S; D012338:RNA, Ribosomal, 23S; D012341:RNA, Ribosomal, 5S