title: The contribution of DNA apurinic/apyrimidinic endonuclease genotype and smoking habit to Taiwan lung cancer risk.
abstract: To evaluate the association and interaction of genotypic polymorphism the gene for DNA-apurinic/apyrimidinic endonuclease (APEX1) with personal smoking habit and lung cancer risk in Taiwan, the polymorphic variants of APEX1, Asp(148)Glu (rs1130409), were analyzed in association with lung cancer risk, and their joint effect with personal smoking habits on lung cancer susceptibility was discussed. In this hospital-based case-control study, 358 patients with lung cancer and 716 cancer-free controls, frequency-matched by age and sex, were recruited and genotyped by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). The results showed that the percentages of TT, TG and GG APEX1 Asp(148)Glu genotypes were not significantly different at 43.0%, 41.1% and 15.9% in the lung cancer patient group and 39.9%, 46.1% and 14.0% in non-cancer control group, respectively. We further analyzed the genetic-lifestyle effects on lung cancer risk and found the contribution of APEX1 Asp(148)Glu genotypes to lung cancer susceptibility was neither enhanced in the cigarette smokers nor in the non-smokers (p=0.3550 and 0.8019, respectively). Our results provide evidence that the non-synonymous polymorphism of APEX1 Asp(148)Glu may not be directly associated with lung cancer risk, nor enhance the effects of smoking habit on lung cancer development.
authors.affiliation: Department of Internal Medicine, China Medical University Hospital, Taichung, Taiwan, ROC.
authors.forename: Wei-Chun
authors.initials: WC
authors.lastname: Chen
authors.forename: Chia-Wen
authors.initials: CW
authors.lastname: Tsai
authors.forename: Te-Chun
authors.initials: TC
authors.lastname: Hsia
authors.forename: Wen-Shin
authors.initials: WS
authors.lastname: Chang
authors.forename: Liang-Yi
authors.initials: LY
authors.lastname: Lin
authors.forename: Shinn-Jye
authors.initials: SJ
authors.lastname: Liang
authors.forename: Chih-Yen
authors.initials: CY
authors.lastname: Tu
authors.forename: Wei-Erh
authors.initials: WE
authors.lastname: Cheng
authors.forename: Hung-Jen
authors.initials: HJ
authors.lastname: Chen
authors.forename: Shu-Ming
authors.initials: SM
authors.lastname: Wang
authors.forename: da-Tian
authors.initials: dT
authors.lastname: Bau
mesh_terms: D016022:Case-Control Studies; D004260:DNA Repair; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008175:Lung Neoplasms / Q000453:epidemiology* / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects*; D013624:Taiwan / Q000453:epidemiology
chemical_list: C085108:APEX1 protein, human; D043603:DNA-(Apurinic or Apyrimidinic Site) Lyase
keywords: APEX1; Taiwanese; lung cancer; single-nucleotide polymorphism; smoking habit