title: Salegentibacter echinorum sp. nov., isolated from the sea urchin Hemicentrotus pulcherrimus.
abstract: A novel Gram-negative, strictly aerobic, heterotrophic, non-motile and yellow-pigmented bacterial strain, designated HD4(T), was isolated from the sea urchin Hemicentrotus pulcherrimus collected from the Yellow Sea in China. Optimal growth of the strain was observed at 28-30 °C, pH 6.8-7.3, and in the presence of 3-5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain HD4(T) exhibited high similarity with the members of Salegentibacter (92.3-95.4 %). The DNA G+C content was 37.0 mol%, MK-6 was the main respiratory quinone and summed feature 3 (comprising iso-C15:0 2-OH/C16:1ω7c), iso-C15:0, iso-C17:0 3-OH and anteiso-C15:0 were the major cellular fatty acids. The predominant polar lipids in strain HD4(T) were phosphatidylethanolamine and two unknown lipids (L2, L4). Based on the phylogenetic, physiological and biochemical characteristics, strain HD4(T) should be classified as a novel species within the genus Salegentibacter, for which the name Salegentibacter echinorum sp. nov. is proposed. The type strain is HD4(T) (=CICC 10466(T) = NRRL B-59666(T)).
authors.affiliation: College of Marine Science, Shandong University at Weihai, Weihai, China.
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Xia
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Li
authors.forename: Qian-Qian
authors.initials: QQ
authors.lastname: Liu
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Miao
authors.forename: Zong-Jun
authors.initials: ZJ
authors.lastname: Du
authors.forename: Guan-Jun
authors.initials: GJ
authors.lastname: Chen
mesh_terms: D000818:Animals; D001420:Bacteria, Aerobic / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D041965:Flavobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D047330:Hemicentrotus / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D010860:Pigments, Biological / Q000378:metabolism; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D010860:Pigments, Biological; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride