title: Effect of salinity tolerant PDH45 transgenic rice on physicochemical properties, enzymatic activities and microbial communities of rhizosphere soils.
abstract: The effect of genetically modified (GM) plants on environment is now major concern worldwide. The plant roots of rhizosphere soil interact with variety of bacteria which could be influenced by the transgene in GM plants. The antibiotic resistance genes in GM plants may be transferred to soil microbes. In this study we have examined the effect of overexpression of salinity tolerant pea DNA helicase 45 (PDH45) gene on microbes and enzymatic activities in the rhizosphere soil of transgenic rice IR64 in presence and absence of salt stress in two different rhizospheric soils (New Delhi and Odisha, India). The diversity of the microbial community and soil enzymes viz., dehydrogenase, alkaline phosphatase, urease and nitrate reductase was assessed. The results revealed that there was no significant effect of transgene expression on rhizosphere soil of the rice plants. The isolated bacteria were phenotyped both in absence and presence of salt and no significant changes were found in their phenotypic characters as well as in their population. Overall, the overexpression of PDH45 in rice did not cause detectable changes in the microbial population, soil enzymatic activities and functional diversity of the rhizosphere soil microbial community.
authors.affiliation: Plant Molecular Biology Group; International Centre for Genetic Engineering and Biotechnology; New Delhi, India.
authors.forename: Ranjan Kumar
authors.initials: RK
authors.lastname: Sahoo
authors.forename: Narendra
authors.initials: N
authors.lastname: Tuteja
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001419:Bacteria / Q000201:enzymology* / Q000254:growth & development / Q000302:isolation & purification; D055598:Chemical Phenomena; D015169:Colony Count, Microbial; D057075:Enzyme Assays; D064307:Microbiota; D009348:Nematoda / Q000302:isolation & purification; D050901:Nitrate Reductase / Q000378:metabolism; D012275:Oryza / Q000235:genetics / Q000502:physiology*; D010940:Plant Proteins / Q000378:metabolism*; D030821:Plants, Genetically Modified; D058441:Rhizosphere; D055049:Salt Tolerance; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology; D014510:Urease / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D012987:Soil; D050901:Nitrate Reductase; D000469:Alkaline Phosphatase; D014510:Urease
keywords: enzymatic activity; microbial community; pea DNA helicase; rice rhizosphere; soil properties; transgenic plants