title: Expression of exoinulinase genes in Saccharomyces cerevisiae to improve ethanol production from inulin sources.
abstract: To improve inulin utilization and ethanol fermentation, exoinulinase genes from the yeast Kluyveromyces marxianus and the recently identified yeast, Candida kutaonensis, were expressed in Saccharomyces cerevisiae. S. cerevisiae harboring the exoinulinase gene from C. kutaonensis gave higher ethanol yield and productivity from both inulin (0.38 vs. 0.34 g/g and 1.35 vs. 1.22 g l(-1) h(-1)) and Jerusalem artichoke tuber flour (0.47 vs. 0.46 g/g and 1.62 vs. 1.54 g l(-1) h(-1)) compared with the strain expressing the exoinulinase gene from K. marxianus. Thus, the exoinulinase gene from C. kutaonensis is advantageous for engineering S. cerevisiae to improve ethanol fermentation from inulin sources.
authors.affiliation: Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China, yuanbo@qibebt.ac.cn.
authors.forename: Bo
authors.initials: B
authors.lastname: Yuan
authors.forename: Shi-An
authors.initials: SA
authors.lastname: Wang
authors.forename: Fu-Li
authors.initials: FL
authors.lastname: Li
mesh_terms: D002175:Candida / Q000201:enzymology / Q000235:genetics; D000431:Ethanol / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism*; D007444:Inulin / Q000378:metabolism*; D007716:Kluyveromyces / Q000201:enzymology / Q000235:genetics; D060847:Metabolic Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D000431:Ethanol; D007444:Inulin; D006026:Glycoside Hydrolases; C023086:inulinase