title: Comprehensive chromosome analysis of blastocysts before implantation using array CGH.
abstract: BACKGROUND
  Chromosomal abnormalities are common in embryos produced in vitro and cause implantation failure, miscarriage, and serious medical problems in infants. Because preimplantation genetic screening (PGS) is increasingly being used to detect aneuploidy in embryos with the purpose of improving implantation rates after IVF (in vitro fertilization), we aimed to validate the usefulness of array CGH for the preimplantation genetic screening (PGS) of embryos at the blastocyst stage of development.
  
  
  RESULTS
  A total of 150 blastocysts were biopsied from couples undergoing IVF and analyzed using array CGH. We found that 54.5% (73/134) of the blastocysts were euploid embryos, whereas 45.5% of the embryos (61/134) had chromosomal abnormalities. Multiple chromosome abnormality was most frequently observed (34.4%), and dual aneuploidy was observed in 26.2% of the embryos. Monosomy (21.3%) appeared more frequently than trisomy (18%).
  
  
  CONCLUSIONS
  Chromosomal microarray analysis provided clinically significant cytogenetic information regarding the frequency and variety of chromosomal abnormalities observed in embryos at the blastocyst stage, suggesting that this is a useful tool for comprehensive aneuploidy screening in IVF.
authors.affiliation: Seoul Rachel Fertility Center, 109 Mapo-daero, Seoul, Korea.
authors.forename: Mi Kyung
authors.initials: MK
authors.lastname: Chung
authors.affiliation: Seoul Rachel Fertility Center, 109 Mapo-daero, Seoul, Korea.
authors.forename: Hyeon Jeong
authors.initials: HJ
authors.lastname: Jeong
authors.affiliation: Seoul Rachel Fertility Center, 109 Mapo-daero, Seoul, Korea.
authors.forename: Jung Hyun
authors.initials: JH
authors.lastname: Lee
authors.affiliation: MGMED, Inc., Elysia bldg, 173, digital-ro, Gasan-dong, Seoul, Korea.
authors.forename: Sang-Jin
authors.initials: SJ
authors.lastname: Park
authors.affiliation: MGMED Clinic, 60-24 Gasan-dong, Seoul, Korea.
authors.forename: Hee-Doo
authors.initials: HD
authors.lastname: Chung
authors.affiliation: MGMED, Inc., Elysia bldg, 173, digital-ro, Gasan-dong, Seoul, Korea.
authors.forename: Ho-Young
authors.initials: HY
authors.lastname: Kang