title: Mast cell tryptase induces eosinophil recruitment in the pleural cavity of mice via proteinase-activated receptor 2.
abstract: Proteinase-activated receptor (PAR) 2 has been implicated in eosinophil migration. Mast cell (MC) tryptase has been similarly implicated in allergic diseases through the activation of PAR-2, but the role of this receptor in MC tryptase-induced inflammation is not well elucidated. This study aims to investigate the ability of MC tryptase or PAR-2 activating peptide (SLIGRL-NH2) to induce eosinophil recruitment to the pleural cavity of mice. Mast cell tryptase-injected mice were pretreated with PAR-2 antagonist ENMD-1068. Mice injected with SLIGRL-NH2 were pretreated with mast cell tryptase inhibitor APC 366, and eosinophil migration into the pleural cavity and PAR-2 expression was analyzed after 24 or 48 h. SLIGRL-NH2-induced eosinophil recruitment was inhibited by APC 366, and MC tryptase-induced eosinophil recruitment was abolished by ENMD-1068. MC tryptase induced PAR-2 expression on pleural eosinophils. Our results demonstrate a key role for PAR-2 in mediating eosinophil recruitment in MC tryptase-induced pleurisy in mice. The ability of MC tryptase to inducing PAR-2 expression on eosinophils corroborates the relevance of MC tryptase and PAR-2 on modulating eosinophil migration.
authors.affiliation: Department of Pharmacology, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Natália A
authors.initials: NA
authors.lastname: Matos
authors.forename: Josiane F
authors.initials: JF
authors.lastname: Silva
authors.forename: Tamires C
authors.initials: TC
authors.lastname: Matsui
authors.forename: Karine A
authors.initials: KA
authors.lastname: Damasceno
authors.forename: Igor D G
authors.initials: ID
authors.lastname: Duarte
authors.forename: Virginia S
authors.initials: VS
authors.lastname: Lemos
authors.forename: Geovanni D
authors.initials: GD
authors.lastname: Cassali
authors.forename: André
authors.initials: A
authors.lastname: Klein
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000276:immunology; D004151:Dipeptides / Q000494:pharmacology; D004804:Eosinophils / Q000276:immunology*; D007249:Inflammation / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009842:Oligopeptides / Q000276:immunology / Q000494:pharmacology; D010879:Piperazines / Q000494:pharmacology; D035422:Pleural Cavity / Q000276:immunology*; D010998:Pleurisy / Q000276:immunology*; D044464:Receptor, PAR-2 / Q000276:immunology*; D053802:Tryptases / Q000276:immunology*
chemical_list: C518710:1-3-methylbutyryl-N4-6-aminohexanoyl-piperazine; D004151:Dipeptides; C094700:N-(1-hydroxy-2-naphthoyl)arginyl-prolinamide; D009842:Oligopeptides; D010879:Piperazines; D044464:Receptor, PAR-2; C112356:seryl-leucyl-isoleucyl-glycyl--arginyl-leucinamide; D053802:Tryptases