title: Technetium 99m-labeled VQ peptide: a new imaging agent for the early detection of tumors or premalignancies.
abstract: There is a critical need to develop diagnostic procedures enabling early detection of tumors while at a curable stage. Technetium 99m (99mTc)-labeled VQ peptide (99mTc-HYNIC-VQ) identified through screening phage display peptide libraries against fresh human colonic adenomas was prepared and evaluated for tumor detection. 99mTc-HYNIC-VQ was prepared by a non-SnCl2 method with more than 99% radiochemical purity. The biodistribution in the HT-29 tumor model showed that although the absolute tumor uptake values were relatively low (0.60 ± 0.09, 0.41 ± 0.09, 0.36 ± 0.18, and 0.19 ± 0.08 %ID/g at 0.5, 1, 2, and 4 hours postinjection, respectively), the tumor uptake was higher than that of any of the other organs except for the kidneys at any time point examined, which led to the high tumor to nontarget ratios. The tumors and inflammation were clearly visualized with high contrast. Although the mechanism of accumulation of radiolabeled VQ peptide in tumors and inflammation needs to be further investigated, 99mTc-HYNIC-VQ is a promising imaging agent for the early detection of tumors or premalignancies, at least for screening patients with a high risk of developing cancers.
authors.affiliation: Medical Isotopes Research Center, Medical and Healthy Analytical Center, and Department of Radiation Medicine, School of Basic Medical Sciences, Peking University, Beijing, China.
authors.forename: Jiyun
authors.initials: J
authors.lastname: Shi
authors.forename: Liyang
authors.initials: L
authors.lastname: Cui
authors.forename: Bing
authors.initials: B
authors.lastname: Jia
authors.forename: Zhaofei
authors.initials: Z
authors.lastname: Liu
authors.forename: Peng
authors.initials: P
authors.lastname: He
authors.forename: Chengyan
authors.initials: C
authors.lastname: Dong
authors.forename: Xiaona
authors.initials: X
authors.lastname: Jin
authors.forename: Huiyun
authors.initials: H
authors.lastname: Zhao
authors.forename: Fang
authors.initials: F
authors.lastname: Li
authors.forename: Fan
authors.initials: F
authors.lastname: Wang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D003952:Diagnostic Imaging; D004151:Dipeptides; D055088:Early Detection of Cancer / Q000379:methods*; D005260:Female; D006801:Humans; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D015609:Organotechnetium Compounds; D010455:Peptides; D013667:Technetium / Q000493:pharmacokinetics; D014018:Tissue Distribution
chemical_list: D004151:Dipeptides; D015609:Organotechnetium Compounds; D010455:Peptides; C587984:technetium 99m HYNIC-VQ; D013667:Technetium