title: Heparin functionalized polyaspartamide/polyester scaffold for potential blood vessel regeneration.
abstract: An interesting issue in tissue engineering is the development of a biodegradable vascular graft able to substitute a blood vessel and to allow its complete regeneration. Here, we report a new scaffold potentially useful as a synthetic vascular graft, produced through the electrospinning of α,β-poly(N-2-hydroxyethyl) (2-aminoethylcarbamate)-D,L-aspartamide-graft-polylactic acid (PHEA-EDA-g-PLA) in the presence of polycaprolactone (PCL). The scaffold degradation profile has been evaluated as well as the possibility to bind heparin to electrospun fibers, being it a known anticoagulant molecule able to bind growth factors. In vitro cell compatibility has been investigated using human vascular endothelial cells (ECV 304) and the ability of heparinized PHEA-EDA-g-PLA/PCL scaffold to retain basic fibroblast growth factor has been evaluated in comparison with not heparinized sample.
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche Chimiche e Farmaceutiche, Plesso di Chimica e Tecnologie Farmaceutiche, Università degli Studi di Palermo, Via Archirafi 32, 90123, Palermo, Italy; IBIM-CNR, Via Ugo La Malfa 153, 90146, Palermo, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Pitarresi
authors.forename: Calogero
authors.initials: C
authors.lastname: Fiorica
authors.forename: Fabio Salvatore
authors.initials: FS
authors.lastname: Palumbo
authors.forename: Salvatrice
authors.initials: S
authors.lastname: Rigogliuso
authors.forename: Giulio
authors.initials: G
authors.lastname: Ghersi
authors.forename: Gaetano
authors.initials: G
authors.lastname: Giammona
mesh_terms: D001218:Aspartame / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D001808:Blood Vessels / Q000187:drug effects / Q000502:physiology*; D049109:Cell Proliferation / Q000187:drug effects; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D016222:Fibroblast Growth Factor 2 / Q000494:pharmacology; D006493:Heparin / Q000494:pharmacology*; D006801:Humans; D019344:Lactic Acid / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D011073:Polyamines / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D011091:Polyesters / Q000494:pharmacology*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D012038:Regeneration / Q000187:drug effects*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D011073:Polyamines; D011091:Polyesters; D011108:Polymers; C509366:alpha,beta-poly(N-2-hydroxyethyl)(2-aminoethylcarbamate)-d,l-aspartamide; D016222:Fibroblast Growth Factor 2; C016240:polycaprolactone; D019344:Lactic Acid; C033616:poly(lactide); D006493:Heparin; D001218:Aspartame
keywords: electrospinning; graft copolymer; heparin; tissue engineering; vascular graft