title: [Optimization of extraction of Clerodendranthus spicatus using response surface methodology].
abstract: OBJECTIVE
  To obtain the optimal conditions of extraction of Clerodendranthus spicatus and provide the theoretical foundation for its further processing and utilization.
  
  
  METHODS
  On the basis of the single factor test, response surface methodology was applied to optimize the extraction conditions.
  
  
  RESULTS
  The results showed that extraction time, water-feed ratio, ethanol concentration and extraction temperature all had significant effects on the extraction rate of polysaccharides. The optimal extraction time was 3h, solid-liquid ratio was 50:1, ethanol concentration was 30% and extraction temperature was 80 degrees C. Under these optimized conditions, the extraction rate was 27.71%.
  
  
  CONCLUSIONS
  The extraction technology is simple, reliable and highly predictive.
authors.affiliation: Yunnan Branch, Institute of Medicinal Plant, Chinese Academy of Medical Sciences, Jinghong 666100.
authors.forename: Er
authors.initials: E
authors.lastname: Li
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.forename: Yi-Hang
authors.initials: YH
authors.lastname: Li
authors.forename: Xue-Lan
authors.initials: XL
authors.lastname: Li
authors.forename: Jun-Ling
authors.initials: JL
authors.lastname: Zhao
authors.forename: Guang
authors.initials: G
authors.lastname: Li
mesh_terms: D002851:Chromatography, High Pressure Liquid; D002934:Cinnamates / Q000032:analysis* / Q000302:isolation & purification; D053630:Depsides / Q000032:analysis* / Q000302:isolation & purification; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D000431:Ethanol / Q000737:chemistry; D019686:Lamiaceae / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013997:Time Factors; D000097123:Rosmarinic Acid
chemical_list: D002934:Cinnamates; D053630:Depsides; D004365:Drugs, Chinese Herbal; D000431:Ethanol; D000097123:Rosmarinic Acid