title: Altered diurnal variation and localization of clock proteins in the remnant kidney of 5/6 nephrectomy rats.
abstract: OBJECTIVE
  To investigate the localization and diurnal variation of clock proteins (BMAL1, PER2) and clock output protein (DBP) in the remnant kidney of 5/6 nephrectomy rats (STNx).
  
  
  METHODS
  Male wistar rats were randomly divided into sham STNx group (Control) and STNx group. Rats were synchronized 12 weeks to the light: dark cycle 12:12 with light on from 07.00 hours (Zeitgeber time ZT 0). Kidneys were collected to detect the localization and expression rhythm of clock proteins (BMAL1, PER2 and DBP) every 4 h throughout the day by immunohistochemistry and Western blotting.
  
  
  RESULTS
  Clock proteins showed diurnal rhythm in the kidney of the control. But diurnal rhythm of clock proteins changed in the STNx rats. Acrophase of BMAL1, DBP and PER2 advanced 4 h, respectively; mesor of clock proteins increased in the STNx rats. BMAL1 was located in endothelial cells of glomerulus and tubular interstitial vasculars, and it was also expressed in nucleus of tubular cells in cortex and medulla. PER2 was mainly expressed in proximal tubular cells at the juncture of cortex and medulla. DBP was widely expressed in the kidney. The localization of BMAL1 and PER2 were changed in remnant kidneys of the STNx group.
  
  
  CONCLUSIONS
  The localization and diurnal variation of BMAL1, DBP and PER2 are changed in remnant kidney of 5/6 nephrectomy rats and are involved in diurnal rhythm of renal function.
authors.affiliation: Department of Nephrology, Wuhan Central Hospital, Wuhan Hubei 430014, China.huang19713052@sina.com
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Huang
authors.forename: Wen-Li
authors.initials: WL
authors.lastname: Chen
authors.forename: Jing-Ping
authors.initials: JP
authors.lastname: Yuan
authors.forename: Yue-Hong
authors.initials: YH
authors.lastname: Yang
authors.forename: Qi-Hui
authors.initials: QH
authors.lastname: Mei
authors.forename: Li-Xia
authors.initials: LX
authors.lastname: Huang
mesh_terms: D056930:ARNTL Transcription Factors / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D002940:Circadian Rhythm / Q000235:genetics; D056925:Circadian Rhythm Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism; D005786:Gene Expression Regulation; D007150:Immunohistochemistry; D007668:Kidney / Q000378:metabolism* / Q000601:surgery*; D008297:Male; D009392:Nephrectomy / Q000379:methods*; D056950:Period Circadian Proteins / Q000378:metabolism; D017440:Photoperiod; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D056930:ARNTL Transcription Factors; D056925:Circadian Rhythm Signaling Peptides and Proteins; C485975:DBP protein, rat; D004268:DNA-Binding Proteins; C513311:Per2 protein, rat; D056950:Period Circadian Proteins; D012333:RNA, Messenger; D014157:Transcription Factors
keywords: 5/6 nephrectomy; BMAL1; DBP; PER2; circadian