title: Invasive African Salmonella Typhimurium induces bactericidal antibodies against O-antigens.
abstract: Nontyphoidal Salmonella are a major and emerging cause of fatal invasive disease in Africa, and are genetically distinct from those found elsewhere in the world. Understanding the targets of protective immunity to these African Salmonellae is key to vaccine development. We immunized mice and rabbits with heat-inactivated wild-type African invasive Salmonella Typhimurium D23580 and rough mutants lacking O-antigen. Wild-type Salmonella, unlike rough bacteria, induced a large bactericidal antibody response mainly against O-antigen. Bactericidal ability of anti-O-antigen antibodies was confirmed following purification by affinity chromatography. The current findings support the development of an O-antigen conjugate vaccine against invasive nontyphoidal Salmonellae for Africa.
authors.affiliation: Novartis Vaccines Institute for Global Health, Via Fiorentina 1, 53100 Siena, Italy. simona.rondini@novartis.com
authors.forename: Simona
authors.initials: S
authors.lastname: Rondini
authors.forename: Luisa
authors.initials: L
authors.lastname: Lanzilao
authors.forename: Francesca
authors.initials: F
authors.lastname: Necchi
authors.forename: Colette M
authors.initials: CM
authors.lastname: O'Shaughnessy
authors.forename: Francesca
authors.initials: F
authors.lastname: Micoli
authors.forename: Allan
authors.initials: A
authors.lastname: Saul
authors.forename: Calman A
authors.initials: CA
authors.lastname: MacLennan
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D001770:Blood Bactericidal Activity; D006801:Humans; D051379:Mice; D019081:O Antigens / Q000276:immunology*; D011817:Rabbits; D012480:Salmonella Infections / Q000276:immunology* / Q000382:microbiology*; D022562:Salmonella Vaccines / Q000008:administration & dosage / Q000276:immunology*; D012486:Salmonella typhimurium / Q000276:immunology* / Q000302:isolation & purification; D014611:Vaccination / Q000379:methods
chemical_list: D000907:Antibodies, Bacterial; D019081:O Antigens; D022562:Salmonella Vaccines
keywords: Conjugate vaccine; NTS; Nontyphoidal Salmonella; O-antigen