title: [Decreasing effect of electro-acupuncture at Sanyin points on inflammatory cytokines in autoimmune prostatitis rats].
abstract: OBJECTIVE
  To observe the changes of inflammatory cytokines in autoimmune prostatitis (AIP) rats treated by electro-acupuncture (EA) at Sanyin points.
  
  
  METHODS
  We selected 40 Wistar male rats in this study, 10 as normal controls, and the other 30 made AIP models by intradermal injection of protein purification liquid from the prostate of allogeneic male rats with dual immune adjuvant. Then we randomly divided the AIP models into a model, a Cernilton control and an EA group of equal number, the latter two groups treated by Cernilton enema and EA, respectively. After 15 days of treatment, all the animals were sacrificed for detection of the levels of TNF-alpha, iNOS, MDA and T-AOC in the prostate tissue.
  
  
  RESULTS
  Compared with the normal controls, the model rats showed significantly elevated TNF-alpha expression ([15.31 +/- 1.36] vs [32.20 +/- 1.65] pg/ml, P < 0.01), iNOS activity ([0.81 +/- 0.33] vs [1.25 +/- 0.23] U/ml, P < 0.01) and MDA content ([0.66 +/- 0.14] vs [0.91 +/- 0.21] nmol/ml, P < 0.05), but markedly reduced T-AOC activity ([1.56 +/- 0.16] vs [1.11 +/- 0.15] U/ml, P < 0.01). In comparison with the model group, the EA group exhibited significantly reduced levels of TNF-alpha ([17.32 +/- 2.69 ] pg/ml, P < 0.01), iNOS ([0.98 +/- 0.5 ] U/ml, P < 0.05) and MDA ([0.70 +/- 0.20] nmol/ml, P < 0.05), but remarkably increased level of T-AOC ([1.44 +/- 0.26] U/ml, P < 0.05).
  
  
  CONCLUSIONS
  Electro-acupuncture at Sanyin points can protect the prostate tissue from morphological damage and reduce inflammatory reaction by decreasing pro-inflammatory cytokine activity, vascular permeability and inflammatory cell infiltration and increasing the activity of the antioxidant defense system.
authors.affiliation: Department of Acupuncture and Massage, Gansu University of Traditional Chinese Medicine, Lanzhou, Gansu 730000, China. yangxingke@126.com
authors.forename: Xing-Ke
authors.initials: XK
authors.lastname: Yan
authors.forename: An-Guo
authors.initials: AG
authors.lastname: Liu
authors.forename: Tian-You
authors.initials: TY
authors.lastname: He
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Kan
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Dong
authors.forename: Jun-Yan
authors.initials: JY
authors.lastname: Wang
authors.forename: Hua-Zhang
authors.initials: HZ
authors.lastname: Li
mesh_terms: D015669:Acupuncture Points; D000818:Animals; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D015671:Electroacupuncture; D007249:Inflammation; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism; D011467:Prostate / Q000378:metabolism; D011472:Prostatitis / Q000209:etiology / Q000378:metabolism* / Q000628:therapy*; D051381:Rats; D017208:Rats, Wistar; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D016207:Cytokines; D014409:Tumor Necrosis Factor-alpha; D008315:Malondialdehyde; D019001:Nitric Oxide Synthase