title: New insights into the subcellular localization of Tubby-like proteins and their participation in the Arabidopsis-Piriformospora indica interaction.
abstract: Tubby-like proteins (TLPs) have been associated with hormone signaling and responses to abiotic and biotic stress in plants. Recently, Arabidopsis thaliana TLP3 was found to translocate from the plasma membrane of cells in response to distinct abiotic stresses, thereby activating cellular signaling. In addition, several AtTLPs were demonstrated to be necessary for normal colonization of roots by the mutualistic fungus Piriformospora indica. Here, we present evidence for the involvement of another two AtTLPs in this interaction. Furthermore, we show that plasma membrane targeting of TLPs might be conserved in other plant species, although we did not find it for all members of the protein family. Finally, the position of a GFP-tag influences the localization of AtTLP3, which needs to be considered when working with TLPs.
authors.affiliation: The School of Life Sciences; University of Warwick; Gibbet Hill Campus; Coventry, UK.
authors.forename: Marco U
authors.initials: MU
authors.lastname: Reitz
authors.forename: Subhash
authors.initials: S
authors.lastname: Pai
authors.forename: Jafargholi
authors.initials: J
authors.lastname: Imani
authors.forename: Patrick
authors.initials: P
authors.lastname: Schäfer
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000378:metabolism* / Q000382:microbiology*; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000378:metabolism*; D001487:Basidiomycota / Q000502:physiology*; D044783:F-Box Proteins / Q000737:chemistry / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D054884:Host-Pathogen Interactions; D008969:Molecular Sequence Data; D019269:Phosphatidylinositol 4,5-Diphosphate / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism / Q000382:microbiology; D021381:Protein Transport; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D044783:F-Box Proteins; D019269:Phosphatidylinositol 4,5-Diphosphate; C000588902:TLP1 protein, Arabidopsis; C000588903:TLP11 protein, Arabidopsis; D049452:Green Fluorescent Proteins
keywords: Arabidopsis; Piriformospora indica; Tubby-like proteins; phosphatidylinositol 4,5-bisphosphate; phospholipase; plasma membrane targeting; subcellular localization