title: Proteome analysis of the plant-pathogenic bacterium Xanthomonas oryzae pv. oryzae.
abstract: The plant-pathogenic bacterium Xanthomonas oryzae pv. oryzae (Xoo) is the causal agent of bacterial blight, which is one of the most serious diseases of rice. Xoo has been studied for over one century, and much has been learned about it, but proteomic investigation has been neglected. In this study, proteome reference maps of Xoo were constructed by two-dimensional gel electrophoresis, and 628 spots in the gels representing 469 different protein species were identified with MALDI-TOF/TOF MS. The identified spots were assigned to 15 functional categories according to the Kyoto Encyclopedia of Genes and Genomes (KEGG) database and the annotations from the National Center for Biotechnology Information (NCBI) database. The data set has been deposited in the World-2DPAGE database (Database ID: 0044). In addition, comparative proteomic analysis revealed that proteins related to the TonB-dependent transportation system and energy metabolism are involved in the phenazine-1-carboxylic acid resistance in Xoo. In conclusion, we have established a proteome database for Xoo and have used this database in a comparative proteomic analysis that identified proteins potentially contributing to phenazine-1-carboxylic acid resistance in Xoo.
authors.affiliation: Nanjing Agricultural University, Nanjing, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Xu
authors.forename: Jianying
authors.initials: J
authors.lastname: Luo
authors.forename: Xiayan
authors.initials: X
authors.lastname: Pan
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Liang
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.forename: Wenjun
authors.initials: W
authors.lastname: Zheng
authors.forename: Changjun
authors.initials: C
authors.lastname: Chen
authors.forename: Yiping
authors.initials: Y
authors.lastname: Hou
authors.forename: Hongyu
authors.initials: H
authors.lastname: Ma
authors.forename: Mingguo
authors.initials: M
authors.lastname: Zhou
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D024881:Drug Resistance, Bacterial; D015180:Electrophoresis, Gel, Two-Dimensional; D012275:Oryza / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology; D010619:Phenazines / Q000494:pharmacology; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014974:Xanthomonas / Q000187:drug effects / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D010619:Phenazines; D020543:Proteome; C037165:1-phenazinecarboxylic acid