title: Direct targeting of proteins to lipid droplets demonstrated by time-lapse live cell imaging.
abstract: A protein that specifically targets lipid droplets (LDs) was created by connecting two domains of nonstructural protein 4B containing amphipathic helices from hepatitis C virus. We demonstrated its direct targeting and accumulation to the LD surface by time-lapse live cell imaging, comparable to those observed with adipose differentiation-related protein.
authors.affiliation: Division of Biochemistry, Department of Biomedical Sciences, Nihon University School of Medicine, 30-1 Oyaguchi-kamimachi, Itabashi-ku, Tokyo 173-8610, Japan. Electronic address: tanaka.torahiko@nihon-u.ac.jp.
authors.forename: Torahiko
authors.initials: T
authors.lastname: Tanaka
authors.forename: Kazumichi
authors.initials: K
authors.lastname: Kuroda
authors.forename: Masanori
authors.initials: M
authors.lastname: Ikeda
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Kato
authors.forename: Kazufumi
authors.initials: K
authors.lastname: Shimizu
authors.forename: Makoto
authors.initials: M
authors.lastname: Makishima
mesh_terms: D002460:Cell Line; D002470:Cell Survival; D016174:Hepacivirus / Q000737:chemistry*; D006801:Humans; D008055:Lipids; D008565:Membrane Proteins / Q000378:metabolism; D018613:Microscopy, Confocal; D000070780:Perilipin-2; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D013379:Substrate Specificity; D013997:Time Factors; D017361:Viral Nonstructural Proteins / Q000737:chemistry
chemical_list: D008055:Lipids; D008565:Membrane Proteins; C080107:NS4B protein, flavivirus; C000602982:PLIN2 protein, human; D000070780:Perilipin-2; D011506:Proteins; D017361:Viral Nonstructural Proteins
keywords: Adipose differentiation-related protein; Hepatitis C virus; Lipid droplets; Nonstructural protein 4B; Time-lapse imaging