title: Taurine prevented cell cycle arrest and restored neurotrophic gene expression in arsenite-treated SH-SY5Y cells.
abstract: The study investigated the effect of taurine on cell viability and neurotrophic gene expression in arsenite-treated human neuroblastoma SH-SY5Y cells. Arsenite-induced intracellular reactive oxygen species (ROS) and interrupted cell cycle in SH-SY5Y cells. In addition, arsenite reduced mitochondria membrane potential (MMP) and decreased neurotrophic gene expressions such as n-myc downstream-regulated gene 4 (NDRG-4), brain-derived neurotrophic factor (BDNF) and sirtuin-1 (SIRT-1) in SH-SY5Y cells. In parallel, taurine prevented cell cycle, restored MMP and reduced the intracellular ROS level, and taurine recovered NDRG-4, BDNF and SIRT-1 gene expressions in arsenite-treated SH-SY5Y cells while taurine alone has no effect on these parameters.
authors.affiliation: Department of Food Science, National Taiwan Ocean University, 2 Pei-Ning Road, Keelung 202, Taiwan, ROC.
authors.forename: Chien-Te
authors.initials: CT
authors.lastname: Chou
authors.forename: Wen-Feng
authors.initials: WF
authors.lastname: Lin
authors.forename: Zwe-Ling
authors.initials: ZL
authors.lastname: Kong
authors.forename: Shiow-Yi
authors.initials: SY
authors.lastname: Chen
authors.forename: Deng-Fwu
authors.initials: DF
authors.lastname: Hwang
mesh_terms: D018053:Arsenites / Q000494:pharmacology*; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics* / Q000378:metabolism; D059447:Cell Cycle Checkpoints / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D009124:Muscle Proteins / Q000235:genetics* / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D056564:Sirtuin 1 / Q000235:genetics* / Q000378:metabolism; D013654:Taurine / Q000494:pharmacology*; D014407:Tumor Cells, Cultured
chemical_list: D018053:Arsenites; D019208:Brain-Derived Neurotrophic Factor; D009124:Muscle Proteins; C431705:NDRG4 protein, human; D009419:Nerve Tissue Proteins; D017382:Reactive Oxygen Species; D013654:Taurine; C579455:BDNF protein, human; C447939:SIRT1 protein, human; D056564:Sirtuin 1; C015001:arsenite