title: Neurotrophin-3 stimulates migration of mesenchymal stem cells overexpressing TrkC.
abstract: OBJECTIVE
  Transplantation/infusion of mesenchymal stem cells (MSCs) is a promising new approach for treatment of spinal cord injury (SCI). Considering some defined chemokines of MSCs that may have adverse side effects in SCI repair, it is therefore desirable to search for a new chemokine, which should not only be harmless to the host, but also would attract more MSCs to the injury area of spinal cord. This study sought to demonstrate if neurotrophin- 3 (NT-3) would attract migration of MSCs with overexpressing tyrosine kinase C (TrkC) a NT-3 receptor.
  
  
  METHODS
  A micropipette containing NT-3 was placed in cell culture dish. After this, movement of TrkC gene modified MSCs was monitored for 240 min under an inverted microscope equipped with an imaging system. In vivo, a gelatin sponge scaffold containing TrkC gene modified MSCs was transplanted into the injury area of transected rat spinal cord. Following this, replication-deficient recombinant adenoviral vectors carrying human NT-3 gene (Ad-NT-3) was injected 1 mm caudal to the transplantation site to create an NT-3 enriched area.
  
  
  RESULTS
  The results showed that TrkC overexpressing MSCs migrated actively towards the source of NT-3 in the NT-3+TrkC-GFP-MSCs group in vitro. A similar phenomenon was not observed in the control groups. In vivo, transplanted MSCs overexpressing TrkC migrated into the NT-3 enriched area. The incidence of migrating MSCs as well as migration distance was significantly higher than the control groups.
  
  
  CONCLUSIONS
  The present results suggest that NT-3 may play a role in attracting MSCs with its high affinity for TrkC as a chemokine receptor.
authors.affiliation: Department of Histology and Embryology, Zhongshan School of Medicine, Sun Yat-sen University, 74# Zhongshan 2nd Road, Guangzhou 510080, China.
authors.forename: Y F
authors.initials: YF
authors.lastname: Chen
authors.forename: X
authors.initials: X
authors.lastname: Zeng
authors.forename: K
authors.initials: K
authors.lastname: Zhang
authors.forename: B Q
authors.initials: BQ
authors.lastname: Lai
authors.forename: E A
authors.initials: EA
authors.lastname: Ling
authors.forename: Y S
authors.initials: YS
authors.lastname: Zeng
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000187:drug effects*; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D020933:Neurotrophin 3 / Q000235:genetics / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020812:Receptor, trkC / Q000096:biosynthesis*; D013119:Spinal Cord Injuries / Q000628:therapy
chemical_list: D020933:Neurotrophin 3; D020812:Receptor, trkC