title: Structural elucidation of a novel water-soluble fructan isolated from Wedelia prostrata.
abstract: A new heteropolysaccharide, WPP60A, with a molecular weight of 2.4 kDa, was isolated from Wedelia prostrata by hot water extraction, and purified by DEAE Sepharose fast flow and Sephacryl S-200 high-resolution chromatography. Compositional analysis, and methylation analysis, combined with (1)H, (13)C NMR spectroscopy including 2D NMR (COSY, TOCSY, HMQC, NOESY, and HMBC) experiments demonstrated that WPP60A was composed of primarily fructose and low glucose. Results indicated that this new heteropolysaccharide consists of a repeating unit with the following structure: [Formula: see text].
authors.affiliation: College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310014, PR China.
authors.forename: An-qiang
authors.initials: AQ
authors.lastname: Zhang
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Jian
authors.initials: J
authors.lastname: Jiang
authors.forename: Fang-fang
authors.initials: FF
authors.lastname: Huang
authors.forename: Pei-long
authors.initials: PL
authors.lastname: Sun
mesh_terms: D002236:Carbohydrate Conformation; D009682:Magnetic Resonance Spectroscopy; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D012995:Solubility; D014867:Water / Q000737:chemistry*; D031209:Wedelia / Q000737:chemistry*
chemical_list: D011134:Polysaccharides; C000592593:WPP60A; D014867:Water