title: Molecular cloning and tumour suppressor function analysis of canine REIC/Dkk-3 in mammary gland tumours.
abstract: REIC/Dkk-3, a member of the human Dickkopf (Dkk) family, plays a role as a suppressor of growth in several human cancers. In this study, the tumour suppression function of canine REIC/Dkk-3 was investigated. The full-length open reading frame of the canine REIC/Dkk-3 homologue was cloned and the tissue distribution of REIC/Dkk-3 mRNA was determined, along with the subcellular localisation of the REIC/Dkk-3 protein in canine cancer cell lines. Expression of REIC/Dkk-3 was lower in mammary gland tumours and in canine mammary carcinoma cell lines than in normal mammary gland tissue. Overexpression of REIC/Dkk-3 induced apoptosis in canine mammary carcinoma cell lines. These results show that expression of REIC/Dkk-3 is downregulated in canine mammary tumours and that one of the functions of this gene is induction of apoptosis.
authors.affiliation: Department of Veterinary Nursing and Technology, School of Veterinary Science, Nippon Veterinary and Life Science University, Tokyo 180-8602, Japan. Electronic address: kochiai@nvlu.ac.jp.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Ochiai
authors.forename: Masami
authors.initials: M
authors.lastname: Watanabe
authors.forename: Daigo
authors.initials: D
authors.lastname: Azakami
authors.forename: Masaki
authors.initials: M
authors.lastname: Michishita
authors.forename: Yasunaga
authors.initials: Y
authors.lastname: Yoshikawa
authors.forename: Chihiro
authors.initials: C
authors.lastname: Udagawa
authors.forename: Pornphimon
authors.initials: P
authors.lastname: Metheenukul
authors.forename: Thippayarat
authors.initials: T
authors.lastname: Chahomchuen
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Aoki
authors.forename: Hiromi
authors.initials: H
authors.lastname: Kumon
authors.forename: Masami
authors.initials: M
authors.lastname: Morimatsu
authors.forename: Toshinori
authors.initials: T
authors.lastname: Omi
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003001:Cloning, Molecular; D004283:Dog Diseases / Q000235:genetics / Q000378:metabolism*; D004285:Dogs; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000502:physiology*; D015674:Mammary Neoplasms, Animal / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D021381:Protein Transport; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D025521:Tumor Suppressor Proteins
keywords: Canine; Mammary gland tumours; REIC/Dkk-3; Tumour suppressor gene