title: Effect of laser phototherapy in the prevention and treatment of chemo-induced mucositis in hamsters.
abstract: The aim of this study was to investigate the effect of laser phototherapy (LPT) in the prevention and/or treatment of oral mucositis induced by 5-fluorouracil (5-FU; Eurofarma, São Paulo, Brazil) in hamsters. Ninety-six hamsters were divided into four groups (n=24): Control (no treatment); Preventive [LPT from day (D) D-5 to D+5]; Therapeutic (LPT from D+5 to D+15); and Combined (preventive plus therapeutic LPT from D-5 to D+15). The animals received an intraperitoneal injection of 5-FU on Days 0 and 2. The pouch mucosa was scratched on Days 3 and 4. The irradiation parameters were: indium-gallium-aluminum-phosphide (InGaAlP) diode laser (MM Optics, São Carlos, Brazil) (660 nm), beam area of 0.036 cm2, 40 mW, 1.11 W/cm2, 6.6 J/cm2, power density applied daily of 39.6 J/cm2, in punctual mode (six points and six seconds per point) and contact mode, one application per day. The animals were sacrificed on Days 0, 5, 10 and 15 (n=6) and weighed, and the pouch mucosa was removed for histopathological analysis. Clinical and corresponding histological scores were compared using ANOVA and Tukey's test (p≤0.05). Similar weight losses ranging from 5% to 10% occurred in all groups. The therapeutic group had significantly lower clinical and histological scores than the other groups at Day 10. This study showed that positive effects on oral mucositis management were obtained only when LPT was applied in the therapeutic protocol (from D+5 to D+15 after chemotherapy).
authors.affiliation: Department of Restorative Dentistry, School of Dentistry, Univ de São Paulo-USP, São Paulo, SP, Brazil.
authors.forename: Talita Christine Camilo
authors.initials: TC
authors.lastname: Lopez
authors.forename: Manoela Domingues
authors.initials: MD
authors.lastname: Martins
authors.forename: Vanessa Christina Santos
authors.initials: VC
authors.lastname: Pavesi
authors.forename: Leila Soares
authors.initials: LS
authors.lastname: Ferreira
authors.forename: Sandra Kalil
authors.initials: SK
authors.lastname: Bussadori
authors.forename: Maria Stella
authors.initials: MS
authors.lastname: Moreira
authors.forename: Márcia Martins
authors.initials: MM
authors.lastname: Marques
mesh_terms: D000818:Animals; D000964:Antimetabolites, Antineoplastic; D006224:Cricetinae; D005472:Fluorouracil; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D011897:Random Allocation; D015203:Reproducibility of Results; D012720:Severity of Illness Index; D013280:Stomatitis / Q000139:chemically induced* / Q000473:pathology / Q000532:radiotherapy*; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D000964:Antimetabolites, Antineoplastic; D005472:Fluorouracil