title: Antioxidant and phenolic acid profiles of tissue cultured and acclimatized Merwilla plumbea plantlets in relation to the applied cytokinins.
abstract: Merwilla plumbea (Lindl.) Speta is an important medicinal plant widely used in traditional medicine. We evaluated the effect of five cytokinins [benzyladenine (BA), 2-isopentenyladenine (2iP), meta-topolin (mT), meta-topolin riboside (mTR), and meta-methoxy-9-tetrahydropyran-2-yl-topolin (MemTTHP)] on the level of phenolic acids and antioxidant activity of M. plumbea during the tissue culture and acclimatization stages. Two cytokinins (mT and mTR) significantly improved the antioxidant activity of tissue culture plantlets while the control plantlets were better after acclimatization. Using UPLC-MS/MS, the levels of hydroxybenzoic and hydroxycinnamic acid derivatives (phenolic acids) varied significantly during tissue culture and acclimatization, depending on the cytokinin and plant part analyzed. Vanillic acid (24.9 μg g⁻¹) detected in underground parts of tissue culture plants supplemented with BA was the most abundant phenolic acid detected. The current findings indicate that the phytochemicals together with the bioactivity during in vitro propagation of M. plumbea is influenced by the cytokinin type used and the stage of plant material collection.
authors.affiliation: Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal, Pietermaritzburg, Private Bag X01, Scottsville 3209, South Africa.
authors.forename: Adeyemi O
authors.initials: AO
authors.lastname: Aremu
authors.forename: Jiří
authors.initials: J
authors.lastname: Gruz
authors.forename: Michaela
authors.initials: M
authors.lastname: Subrtová
authors.forename: Lucie
authors.initials: L
authors.lastname: Szüčová
authors.forename: Karel
authors.initials: K
authors.lastname: Doležal
authors.forename: Michael W
authors.initials: MW
authors.lastname: Bairu
authors.forename: Jeffrey F
authors.initials: JF
authors.lastname: Finnie
authors.forename: Johannes
authors.initials: J
authors.lastname: Van Staden
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D003583:Cytokinins / Q000494:pharmacology*; D062385:Hydroxybenzoates / Q000378:metabolism*; D007541:Isopentenyladenosine / Q000494:pharmacology; D019668:Liliaceae / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D003583:Cytokinins; D062385:Hydroxybenzoates; C001478:N(6)-(delta(2)-isopentenyl)adenine; D007541:Isopentenyladenosine; C017616:phenolic acid
keywords: 2,2′-azobis(2-methylpropionamidine) dihydrochloride; 2-isopentenyladenine; 2iP; AAPH; ANOVA; BA; CK; Conservation; DW; MS; Medicinal plants; MemTTHP; Micropropagation; Murashige and Skoog medium; ORAC; PPFD; Phytochemicals; TE; Topolins; UPLC; analysis of variance; benzyladenine; cytokinin; dry weight; mT; mTR; meta-methoxy-9-tetrahydropyran-2-yl-topolin; meta-topolin; meta-topolin riboside; oxygen radical absorbance capacity; photosynthetic photon flux density; trolox equivalents; ultra performance liquid chromatography