title: Unique microRNA expression profiles in cervical cancer.
abstract: Cervical cancer is the second leading cause of death among female patients with cancer in the world. Our aim was to analyze cervical cancer cases, in the Southwestern Transdanubian Region of Hungary, with regard to human papillomavirus (HPV) genotype and histological and clinical grading. After HPV testing and genotyping, the expressions of eight different pre-microRNAs (miR-21, miR-27a, miR-34a, miR-146a, miR-155, miR-196a, miR-203, miR-221) in formalin-fixed paraffin-embedded (FFPE) primary human cervical cancer samples were evaluated with the help of the LightCycler 480 PCR System (Roche). Statistically significant overexpression of miR-21 (p=0.004), miR-27a (p=0.018), miR-34a (p<0.001), miR-155 (p=0.021), miR-196a (p=0.032), miR-203 (p=0.037) and miR-221 (p=0.017) were observed in squamous cell carcinoma, regardless of HPV status and clinical grading. Significant overexpression of miR-21 (p=0.004), miR-27a (p=0.02), miR-34a (p<0.001), miR-196a (p=0.027) and miR-221 (p=0.031) was characteristic of HPV-positive squamous cell carcinomas in contrast to adenocarcinomas of the same HPV status.
authors.affiliation: Institute of Public Health, University of Pecs, Pecs, Hungary. katag@hotmail.com
authors.forename: Katalin
authors.initials: K
authors.lastname: Gocze
authors.forename: Katalin
authors.initials: K
authors.lastname: Gombos
authors.forename: Krisztina
authors.initials: K
authors.lastname: Juhasz
authors.forename: Krisztina
authors.initials: K
authors.lastname: Kovacs
authors.forename: Bela
authors.initials: B
authors.lastname: Kajtar
authors.forename: Marta
authors.initials: M
authors.lastname: Benczik
authors.forename: Peter
authors.initials: P
authors.lastname: Gocze
authors.forename: Balazs
authors.initials: B
authors.lastname: Patczai
authors.forename: Istvan
authors.initials: I
authors.lastname: Arany
authors.forename: Istvan
authors.initials: I
authors.lastname: Ember
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000821:virology; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000821:virology; D002584:Cervix Uteri / Q000378:metabolism; D005260:Female; D005838:Genotype; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D060787:Neoplasm Grading; D027383:Papillomaviridae / Q000145:classification / Q000235:genetics; D030361:Papillomavirus Infections / Q000235:genetics / Q000473:pathology / Q000821:virology*; D002583:Uterine Cervical Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology / Q000821:virology
chemical_list: D035683:MicroRNAs
keywords: Cervical cancer; HPV; clinical grading; microRNA expression