title: Benzo(a)pyrene modulates fluoranthene-induced cellular responses in HT-29 colon cells in a dual exposure system.
abstract: Our environment is contaminated with a diverse array of chemicals; one of which is polycyclic aromatic hydrocarbons (PAHs). While some PAHs are potent by nature, others undergo interactions such as additivity, synergism, antagonism or potentiation to manifest their toxicity. Therefore, the objective of this study was to investigate whether exposure to benzo(a)pyrene (BaP), a PAH compound influences the cytotoxicity and metabolism of fluoranthene (FLA; another PAH compound) using HT-29 cells. Cells cultured in Dulbecco's Modified Eagle Medium were treated with 1, 5, 10, 25μM BaP and FLA (0.01% dimethylsulfoxide as vehicle) individually and in combination over the course of 0-96h. At the end of exposure, cells were stained with propidium iodide and the changes in cell cycle were analyzed using FACS analysis. Apoptosis was determined by caspase-3 assay. Post-incubation, samples were extracted and analyzed for FLA metabolites by reverse-phase HPLC with fluorescence detection. Cells exposed to BaP+FLA showed a marginal decrease in growth as compared to FLA alone and vehicle controls. Also, a decline in the percentage of cells in the S and G2 phases compared to G1 phase of cell cycle was noted when cells were treated with BaP and FLA together, compared to individual FLA treatment. The rate of FLA metabolism was more when cells were exposed to FLA in combination with BaP, compared to FLA alone. The enhanced biotransformation of FLA as a result of concomitant exposure to BaP may have implications for colon cancer risks arising from human dietary exposure to PAH mixtures through consumption of barbecued meat.
authors.affiliation: Department of Biochemistry & Cancer Biology, Meharry Medical College, 1005 D.B. Todd Boulevard, Nashville, TN 37208, USA.
authors.forename: Kelly L
authors.initials: KL
authors.lastname: Harris
authors.affiliation: Department of Biochemistry & Cancer Biology, Meharry Medical College, 1005 D.B. Todd Boulevard, Nashville, TN 37208, USA.
authors.forename: Jeremy N
authors.initials: JN
authors.lastname: Myers
authors.affiliation: Department of Biochemistry & Cancer Biology, Meharry Medical College, 1005 D.B. Todd Boulevard, Nashville, TN 37208, USA. Electronic address: aramesh@mmc.edu.
authors.forename: Aramandla
authors.initials: A
authors.lastname: Ramesh
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D017209:Apoptosis / Q000187:drug effects; D001564:Benzo(a)pyrene / Q000633:toxicity*; D001711:Biotransformation; D002273:Carcinogens / Q000633:toxicity*; D053148:Caspase 3 / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology*; D003296:Cooking; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D005449:Fluorenes / Q000378:metabolism / Q000633:toxicity*; D019073:HT29 Cells; D006801:Humans; D008460:Meat; D013997:Time Factors
chemical_list: D002273:Carcinogens; D005449:Fluorenes; D001564:Benzo(a)pyrene; C007738:fluoranthene; C505407:CASP3 protein, human; D053148:Caspase 3
keywords: Benzo(a)pyrene; Fluoranthene; Metabolism; Polycyclic aromatic hydrocarbons