title: Surface tunable polymersomes loaded with magnetic contrast agent and drug for image guided cancer therapy.
abstract: Polymersomes with different surface charges were synthesized from polysuccinimide (p) by introducing positively charged polyethylenimine (PEI-P), neutrally charged polyethylene glycol (PEG-P), and negatively charged glycine (GLY-P) to the polymer backbone polysuccinimide (P). Then, the polymersomes were prepared with super paramagnetic iron nanoparticles (SPIONs) to obtain PEI-P encapsulating SPIONs (PEI-PS), PEG-P encapsulating SPIONs (PEG-PS), and GLY-P encapsulating SPIONs (GLY-PS), respectively. The average particle sizes of GLY-PS, PEG-PS, and PEI-PS were analyzed by dynamic light scattering, and it was around 163.nm, 105 nm, and 285 nm, respectively. The surface charges of GLY-PS, PEG-PS, and PEI-PS was found to be -29.5, -18.9, and +44, respectively. The presence of PEI, PEG, and GLY in the polymer backbone was confirmed with nuclear magnetic resonance (NMR). The GLY-PS, PEG-PS, and PEI-PS were loaded with the anticancer drug paclitaxel during the preparation. The drug release from the PEG-PS was faster compared to GLY-PS and PEI-PS. An in vivo hemi-spleen mouse metastatic liver model was established and imaged with MRI after intravenous administration of GLY-PS, PEG-PS, and PEI-PS. From the T2-weighted imaging, it was evident that PEG-PS accumulated in the spleen and liver more efficiently than the other charged formulations of GLY-PS and PEI-PS. From this study, the nanoparticle-based delivery and imaging of anti-cancer drugs could be effectively demonstrated simultaneously.
authors.affiliation: Department of Biomedical Sciences and Center for Biomedical Human Resources (BK-21), Chonnam National University Medical School, 60 Baekseo-Ro, Gwangju 501-757, South Korea.
authors.forename: Muthunarayanan
authors.initials: M
authors.lastname: Muthiah
authors.forename: Sang Joon
authors.initials: SJ
authors.lastname: Lee
authors.forename: Myeongju
authors.initials: M
authors.lastname: Moon
authors.forename: Hyun Jin
authors.initials: HJ
authors.lastname: Lee
authors.forename: Woo Kyun
authors.initials: WK
authors.lastname: Bae
authors.forename: Ik Joo
authors.initials: IJ
authors.lastname: Chung
authors.forename: Yong Yeon
authors.initials: YY
authors.lastname: Jeong
authors.forename: In-Kyu
authors.initials: IK
authors.lastname: Park
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry*; D045744:Cell Line, Tumor; D003287:Contrast Media; D006801:Humans; D008279:Magnetic Resonance Imaging; D051379:Mice; D008807:Mice, Inbred BALB C; D009369:Neoplasms / Q000188:drug therapy*; D011108:Polymers / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D003287:Contrast Media; D011108:Polymers