title: The effect of subambient to elevated atmospheric CO₂ concentration on vascular function in Helianthus annuus: implications for plant response to climate change.
abstract: Plant gas exchange is regulated by stomata, which coordinate leaf-level water loss with xylem transport. Stomatal opening responds to internal concentrations of CO₂ in the leaf, but changing CO₂ can also lead to changes in stomatal density that influence transpiration. Given that stomatal conductance increases under subambient concentrations of CO₂ and, conversely, that plants lose less water at elevated concentrations, can downstream effects of atmospheric CO₂ be observed in xylem tissue? We approached this problem by evaluating leaf stomatal density, xylem transport, xylem anatomy and resistance to cavitation in Helianthus annuus plants grown under three CO₂ regimes ranging from pre-industrial to elevated concentrations. Xylem transport, conduit size and stomatal density all increased at 290 ppm relative to ambient and elevated CO₂ concentrations. The shoots of the 290-ppm-grown plants were most vulnerable to cavitation, whereas xylem cavitation resistance did not differ in 390- and 480-ppm-grown plants. Our data indicate that, even as an indirect driver of water loss, CO₂ can affect xylem structure and water transport by coupling stomatal and xylem hydraulic functions during plant development. This plastic response has implications for plant water use under variable concentrations of CO₂, as well as the evolution of efficient xylem transport.
authors.affiliation: Department of Ecology and Evolutionary Biology, University of California, Santa Cruz, CA, 95064, USA.
authors.forename: Christopher
authors.initials: C
authors.lastname: Rico
authors.affiliation: Department of Ecology and Evolutionary Biology, University of California, Santa Cruz, CA, 95064, USA.
authors.forename: Jarmila
authors.initials: J
authors.lastname: Pittermann
authors.affiliation: United States Department of Agriculture, Grassland Soil and Water Research Laboratory, 808 E. Blackland Rd, Temple, TX, 76502, USA.
authors.forename: H Wayne
authors.initials: HW
authors.lastname: Polley
authors.affiliation: Hawkesbury Institute for the Environment, University of Western Sydney, Richmond, NSW, 2753, Australia.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Aspinwall
authors.affiliation: United States Department of Agriculture, Grassland Soil and Water Research Laboratory, 808 E. Blackland Rd, Temple, TX, 76502, USA.
authors.forename: Phillip A
authors.initials: PA
authors.lastname: Fay
mesh_terms: D001272:Atmosphere / Q000737:chemistry*; D002245:Carbon Dioxide / Q000494:pharmacology*; D057231:Climate Change; D006368:Helianthus / Q000187:drug effects* / Q000502:physiology*; D052585:Phloem / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology; D018547:Plant Stems / Q000187:drug effects / Q000502:physiology; D054046:Plant Stomata / Q000187:drug effects / Q000502:physiology; D018526:Plant Transpiration / Q000187:drug effects; D058526:Plant Vascular Bundle / Q000033:anatomy & histology / Q000187:drug effects* / Q000502:physiology*; D052584:Xylem / Q000033:anatomy & histology / Q000187:drug effects / Q000502:physiology
chemical_list: D002245:Carbon Dioxide
keywords: cavitation; phloem; stomatal density; xylem anatomy; xylem plasticity