title: Lipid peroxidation due to in vitro and in vivo exposure of biological samples to nanoparticles.
abstract: The increasing use of nanomaterials in biological applications raises numerous concerns about the dangers they might pose to living organisms. The rise in oxidative stress is usually the most readily observed effect induced by nanoparticles, with the measurement of lipid peroxidation levels being one of the most frequently used biological markers for its evaluation. Here, we describe the spectrophotometric and fluorimetric methods for determining the modifications of the malondialdehyde (MDA) level induced by many types of nanoparticles in in vitro and in vivo biological systems.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Bucharest, Bucharest, Romania.
authors.forename: Anca
authors.initials: A
authors.lastname: Dinischiotu
authors.forename: Loredana
authors.initials: L
authors.lastname: Stanca
authors.forename: Daniela
authors.initials: D
authors.lastname: Gradinaru
authors.forename: Sorina Nicoleta
authors.initials: SN
authors.lastname: Petrache
authors.forename: Mihaela
authors.initials: M
authors.lastname: Radu
authors.forename: Andreea Iren
authors.initials: AI
authors.lastname: Serban
mesh_terms: D000818:Animals; D002138:Calibration; D002347:Carps; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D004912:Erythrocytes / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation; D008099:Liver / Q000378:metabolism; D008315:Malondialdehyde / Q000737:chemistry* / Q000378:metabolism; D018482:Muscle, Skeletal / Q000378:metabolism; D018384:Oxidative Stress; D045663:Quantum Dots / Q000633:toxicity*; D012015:Reference Standards; D013050:Spectrometry, Fluorescence / Q000592:standards
chemical_list: D008315:Malondialdehyde