title: SC5b-9-induced pulmonary microvascular endothelial hyperpermeability participates in ventilator-induced lung injury.
abstract: Mechanical ventilation with large tidal volumes can increase lung alveolar permeability and initiate inflammatory responses, termed ventilator-induced lung injury (VILI). VILI is characterized by an influx of inflammatory cells, increased pulmonary permeability, and endothelial and epithelial cell death. But the underlying molecular mechanisms that regulate VILI remain unclear. The purpose of this study was to investigate the mechanisms that regulate pulmonary endothelial barrier in an animal model of VILI. These data suggest that SC5b-9, as the production of the complement activation, causes increase in rat pulmonary microvascular permeability by inducing activation of RhoA and subsequent phosphorylation of myosin light chain and contraction of endothelial cells, resulting in gap formation. In general, the complement-mediated increase in pulmonary microvascular permeability may participate in VILI.
authors.affiliation: Department of Diving Medicine, Faculty of Nautical Medicine, Second Military Medical University, Shanghai, 200433, China.
authors.forename: Kan
authors.initials: K
authors.lastname: Liu
authors.forename: Yan-Fei
authors.initials: YF
authors.lastname: Mao
authors.forename: Juan
authors.initials: J
authors.lastname: Zheng
authors.forename: Zhao-Yun
authors.initials: ZY
authors.lastname: Peng
authors.forename: Wen-Wu
authors.initials: WW
authors.lastname: Liu
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
authors.forename: Wei-Gang
authors.initials: WG
authors.lastname: Xu
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Sun
authors.forename: Chun-Lei
authors.initials: CL
authors.lastname: Jiang
authors.forename: Lai
authors.initials: L
authors.lastname: Jiang
mesh_terms: D000818:Animals; D002463:Cell Membrane Permeability / Q000187:drug effects; D002478:Cells, Cultured; D015938:Complement Membrane Attack Complex / Q000494:pharmacology*; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D018994:Myosin Light Chains / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D055397:Ventilator-Induced Lung Injury / Q000209:etiology* / Q000378:metabolism; D020742:rhoA GTP-Binding Protein / Q000378:metabolism
chemical_list: D015938:Complement Membrane Attack Complex; D018994:Myosin Light Chains; C047610:SC5b-9 protein complex; D020742:rhoA GTP-Binding Protein