title: Development of a rapid chemiluminescent ciELISA for simultaneous determination of florfenicol and its metabolite florfenicol amine in animal meat products.
abstract: BACKGROUND
  A rapid one-step chemiluminescent competitive indirect enzyme-linked immunosorbent assay (CL-ciELISA) for florfenicol (FF) and its major metabolite florfenicol amine (FFA) residues in animal meat products has been developed.
  
  
  RESULTS
  The 50% binding inhibition (IC₅₀) values of the method were 0.195 µg kg⁻¹ for FFA and 0.24 µg kg⁻¹ for FF under optimum conditions. The cross-reactive rates for FF and FFA were 100.0% and 81.2%, respectively. FF and FFA were easily extracted from animal meat product with an FF/FFA extraction buffer, obtaining recoveries of 81.8-92.0% (FF) and 77.2-100% (FFA). The whole one-step CL-ciELISA test can be accomplished within 40 min in theory. The detection limits (LODs) of the assay were 0.98 µg kg⁻¹ for FF and 0.80 µg kg⁻¹ for FFA in animal meat samples. Finally, field animal meat samples were analyzed with the CL-ciELISA method, and the results correlated well with those obtained using traditional ELISA and a previously reported liquid chromatographic-tandem mass spectrometric method.
  
  
  CONCLUSIONS
  The combined results confirmed the utility of this faster one-step CL-ciELISA for simultaneous trace analysis of FF and FFA. To date, this is the most rapid developed ELISA and CL-ELISA method for detection of FF and FFA.
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.
authors.forename: Xiaoqi
authors.initials: X
authors.lastname: Tao
authors.forename: Xuezhi
authors.initials: X
authors.lastname: Yu
authors.forename: Dongdong
authors.initials: D
authors.lastname: Zhang
authors.forename: Weimin
authors.initials: W
authors.lastname: Shi
authors.forename: Haiyang
authors.initials: H
authors.lastname: Jiang
authors.forename: Xia
authors.initials: X
authors.lastname: Wang
authors.forename: Zhanhui
authors.initials: Z
authors.lastname: Wang
authors.forename: Lanlan
authors.initials: L
authors.lastname: Niu
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Wu
authors.forename: Xi
authors.initials: X
authors.lastname: Xia
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Shen
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods; D008461:Meat Products / Q000032:analysis*; D013839:Thiamphenicol / Q000031:analogs & derivatives* / Q000032:analysis
chemical_list: D000900:Anti-Bacterial Agents; C083312:florfenicol amine; C035534:florfenicol; D013839:Thiamphenicol
keywords: animal meat product; chemiluminescent ciELISA; florfenicol; florfenicol amine; one-step