title: Self-assembly of DNA-based drug delivery nanocarriers with rolling circle amplification.
abstract: DNA nanostructures have recently emerged as a type of drug delivery nanocarriers due to their suitable sizes, well-defined structures and low-toxicity. Here, we present a protocol for the assembly of DNA nanoribbon structures with rolling circle amplification (RCA) and delivery of CpG oligonucleotide. DNA nanoribbons with different dimensions and patterns were assembled with long RCA strands and several short staples. Significantly, we demonstrated they exhibited high-efficiency cellular uptake and improved immunostimulatory activity compared with ss- or ds- DNA.
authors.affiliation: Division of Physical Biology, Bioimaging Center, Shanghai Synchrotron Radiation Facility (SSRF), Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
authors.forename: Xiangyuan
authors.initials: X
authors.lastname: Ouyang
authors.affiliation: Division of Physical Biology, Bioimaging Center, Shanghai Synchrotron Radiation Facility (SSRF), Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Li
authors.affiliation: Division of Physical Biology, Bioimaging Center, Shanghai Synchrotron Radiation Facility (SSRF), Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
authors.forename: Huajie
authors.initials: H
authors.lastname: Liu
authors.affiliation: Division of Physical Biology, Bioimaging Center, Shanghai Synchrotron Radiation Facility (SSRF), Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhao
authors.affiliation: National Engineering Research Center for Nanotechnology (NERCN), Shanghai 200241, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Yan
authors.affiliation: National Engineering Research Center for Nanotechnology (NERCN), Shanghai 200241, China.
authors.forename: Dannong
authors.initials: D
authors.lastname: He
authors.affiliation: Division of Physical Biology, Bioimaging Center, Shanghai Synchrotron Radiation Facility (SSRF), Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China. Electronic address: fchh@sinap.ac.cn.
authors.forename: Chunhai
authors.initials: C
authors.lastname: Fan
authors.affiliation: Division of Physical Biology, Bioimaging Center, Shanghai Synchrotron Radiation Facility (SSRF), Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China. Electronic address: chaojie@sinap.ac.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Chao
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D017104:Bacteriophage M13 / Q000235:genetics; D001483:Base Sequence; D002460:Cell Line; D018899:CpG Islands; D004277:DNA, Single-Stranded / Q000737:chemistry*; D004279:DNA, Viral / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry* / Q000494:pharmacology; D051379:Mice; D021141:Nucleic Acid Amplification Techniques; D009690:Nucleic Acid Conformation; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000276:Adjuvants, Immunologic; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D004337:Drug Carriers; D009838:Oligodeoxyribonucleotides; D014409:Tumor Necrosis Factor-alpha
keywords: DNA nanoribbons; Intracellular delivery; Nucleic acids; Rolling circle amplification