title: Maximizing antibody production in suspension-cultured mammalian cells by the customized transient gene expression method.
abstract: Due to the great diversity in protein expression productivity, a customized transient gene expression (TGE) method was used in the present study to optimize transient expression of three antibodies. Several factors, including host cells, temperature, valproic acid (VPA) treatment, various vectors, and additives were optimized independently and then combined to form a customized TGE protocol for each antibody. In the event, the optimized TGE conditions for three antibodies were different from each other. Compared with the TGE in CHO-S cells by pCDNA3.1 expression vector, the expression productivities of 8C11 cAb, 37 hAb, and 10F7 cAb showed 16-fold, 293-fold, and 19-fold increases respectively by the customized TGE method. For 8C11 cAb, coexpressing L-chain and H-chain on different plasmids led to higher yields. The customized TGE method is an alternative approach that can greatly improve the expression productivity of a variety of recombinant proteins.
authors.affiliation: National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Life Sciences, Xiamen University, Xiamen, China.
authors.forename: Min
authors.initials: M
authors.lastname: You
authors.forename: Yanning
authors.initials: Y
authors.lastname: Liu
authors.forename: Yingwei
authors.initials: Y
authors.lastname: Chen
authors.forename: Jiyuan
authors.initials: J
authors.lastname: Guo
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
authors.forename: Yajuan
authors.initials: Y
authors.lastname: Fu
authors.forename: Rong
authors.initials: R
authors.lastname: Shen
authors.forename: Rui
authors.initials: R
authors.lastname: Qi
authors.forename: Wenxin
authors.initials: W
authors.lastname: Luo
authors.forename: Ningshao
authors.initials: N
authors.lastname: Xia
mesh_terms: D000818:Animals; D000906:Antibodies / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D000917:Antibody Formation; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D015870:Gene Expression / Q000187:drug effects / Q000276:immunology*; D005822:Genetic Vectors; D008322:Mammals; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D014162:Transfection; D014635:Valproic Acid / Q000494:pharmacology
chemical_list: D000906:Antibodies; D011994:Recombinant Proteins; D014635:Valproic Acid