title: Cobalt(III) as a stable and inert mediator ion between NTA and His6-tagged proteins.
abstract: Don't let go! The Co(3+)-mediated interaction between nitrilotriacetic acid (NTA) and the His6-tag is so stable and inert towards ligand exchange that it has a half-life of 7 days in the presence of imidazole and survives even under strongly chelating as well as reducing conditions, unlike the commonly used Ni(2+) or Co(2+) complexes. Possible applications include the separation of labeled proteins and the stable immobilization of proteins on surfaces.
authors.affiliation: Abt. Neue Materialieln und Biosysteme, Max-Planck-Institut für Intelligente Systeme, Heisenbergstrasse 3, 70596, Stuttgart, Germany.
authors.forename: Seraphine V
authors.initials: SV
authors.lastname: Wegner
authors.forename: Joachim P
authors.initials: JP
authors.lastname: Spatz
mesh_terms: D003035:Cobalt / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000737:chemistry / Q000378:metabolism; D006639:Histidine / Q000737:chemistry*; D055767:Immobilized Proteins / Q000737:chemistry; D007477:Ions / Q000737:chemistry; D007700:Kinetics; D009571:Nitrilotriacetic Acid / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: C471213:His-His-His-His-His-His; D055767:Immobilized Proteins; D007477:Ions; D009842:Oligopeptides; D011506:Proteins; D049452:Green Fluorescent Proteins; D003035:Cobalt; D006639:Histidine; D009571:Nitrilotriacetic Acid
keywords: His-tag; cobalt; kinetics; nitrilotriacetic acid; protein modification