title: Aliidiomarina sanyensis sp. nov., a hexabromocyclododecane assimilating bacterium from the pool of Spirulina platensis cultivation, Sanya, China.
abstract: A novel Gram-negative, rod shaped, motile, non-spore-forming, aerobic, brominated flame retardant hexabromocyclododecane-assimilating bacterium, designated strain GYP-17(T), was isolated from a pool of marine Spirulina platensis cultivation, Sanya, China. Colonies on 1/10 strength of marine Glycerol Enriched Medium plates were circular, dark-brown, 1-2 mm in diameter, and with regular margins. Growth occurred at 10-45 °C, 1-10 % (w/v) NaCl and pH of 7-9. The polar lipids were composed of phosphatidylethanolamine, three unidentified phospholipids and one unidentified polar lipid. The major fatty acids were iso-C17:1ω9c/10-methyl-C16:0 (summed feature 9, 20.75 %), iso-C15:0 (17.70 %) and C16:0 (6.40 %). The major respiratory quinone was Q-8. The DNA G + C content of the type strain was 53.6 mol%. Phylogenetic analysis revealed that strain GYP-17(T) was a member of the genus Aliidiomarina and closely related to Aliidiomarina haloalkalitolerans with a 16S rDNA sequence similarity of 96.36 %. Results from the polyphasic taxonomy study support the conclusion that strain GYP-17(T) represents a novel Aliidiomarina species, for which the name Aliidiomarina sanyensis sp. nov. is proposed. The type strain of A. sanyensis is GYP-17(T) (=KCTC 32218(T) =LMG 27471(T)).
authors.affiliation: Key Laboratory of Marine Bio-resources Sustainable Utilization, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China.
authors.forename: Guanghua
authors.initials: G
authors.lastname: Wang
authors.forename: Hualian
authors.initials: H
authors.lastname: Wu
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Zhang
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.forename: Xi
authors.initials: X
authors.lastname: Yang
authors.forename: Xinpeng
authors.initials: X
authors.lastname: Tian
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.forename: Wenzhou
authors.initials: W
authors.lastname: Xiang
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
mesh_terms: D001420:Bacteria, Aerobic / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000378:metabolism; D020563:Gammaproteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D006842:Hydrocarbons, Brominated / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008124:Locomotion; D008969:Molecular Sequence Data; D010743:Phospholipids / Q000378:metabolism; D010802:Phylogeny; D010860:Pigments, Biological / Q000378:metabolism; D011809:Quinones / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D006842:Hydrocarbons, Brominated; D010743:Phospholipids; D010860:Pigments, Biological; D011809:Quinones; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride; C089796:hexabromocyclododecane