title: Effect of vitrification of mouse oocyte on the behavior of adult offspring.
abstract: OBJECTIVE
  To evaluate the effect of vitrification of mouse oocytes on the behavior of adult offspring.
  
  
  METHODS
  Oocytes from mice were vitrified, warmed and inseminated, and two-cell embryos were transferred to foster mothers. The behavioral characterization of the offspring was detected by the Morris water maze test, forced swimming test, and elevated plus maze test, and compared to that of offspring from fresh oocytes.
  
  
  RESULTS
  Offspring produced by vitrified oocytes showed normal motor function. In the Morris water maze test of spatial learning there was a slightly decreased time spent in the quadrant containing the platform relative to mice from fresh oocytes, but the difference did not reach statistical significance. In addition, offspring from vitrified oocytes did not exhibit alterations in emotional behavior.
  
  
  CONCLUSIONS
  No alterations were found in the behavioral characterization of adult offspring from vitrified oocytes compared with those from fresh oocytes.
authors.affiliation: Department of Obstetrics and Gynecology, People's Hospital of Linyi City, China.
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Liu
authors.forename: Gui-Hua
authors.initials: GH
authors.lastname: Jia
authors.forename: Gang
authors.initials: G
authors.lastname: Zhang
authors.forename: Kai-Yong
authors.initials: KY
authors.lastname: Tian
authors.forename: Hong-Xia
authors.initials: HX
authors.lastname: Wang
authors.forename: Wen-Yu
authors.initials: WY
authors.lastname: Zhong
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Zhang
authors.forename: Xi-Lan
authors.initials: XL
authors.lastname: Lu
authors.forename: Jun-Tao
authors.initials: JT
authors.lastname: Li
authors.forename: Jian-Min
authors.initials: JM
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001007:Anxiety; D001522:Behavior, Animal; D015925:Cryopreservation; D005260:Female; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009865:Oocytes; D011247:Pregnancy; D011897:Random Allocation; D013550:Swimming
keywords: Adult offspring; Behavior; Mouse oocyte; Vitrification