title: Transient neonatal diabetes mellitus in a Turkish patient with three novel homozygous variants in the ZFP57 gene.
abstract: Neonatal diabetes mellitus (NDM) is a rare form of diabetes that presents within the first six months of life. Nearly 70% of these cases have loss of methylation at the differentially methylated region on chromosome 6q24. To describe the findings in a Turkish male patient with NDM caused by a loss of methylation at chromosome 6q24 and three novel homozygous mutations in the ZFP57 gene, methylation-specific PCR was carried out at 6q24 and mutation analysis of ZFP57 gene was maintained by direct sequencing. Sequencing of ZFP57 gene revealed the hypomethylation of chromosome 6q24 and three novel mutations (chr6:29.641.413 A>T, 29.641.073 C>T, and 29.640.855 G>C), respectively. The latter mutation seems to display the patient's condition due to a highly conservative amino acid substitution in the protein. We suggest the ZFP57 gene as a causative factor for NDM and it should be considered in genetic testing. Further studies including functional analysis of the detected mutations will provide precise information regarding the effect of the mutations.
authors.affiliation: Şişli Etfal Education and Research Hospital, Division of Pediatric Endocrinology, İstanbul, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Boyraz
authors.forename: Korkut
authors.initials: K
authors.lastname: Ulucan
authors.forename: Necati
authors.initials: N
authors.lastname: Taşkın
authors.forename: Teoman
authors.initials: T
authors.lastname: Akçay
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Flanagan
authors.forename: Deborah J G
authors.initials: DJ
authors.lastname: Mackay
mesh_terms: D000595:Amino Acid Sequence; D002896:Chromosomes, Human, Pair 6 / Q000235:genetics; D019175:DNA Methylation; D004252:DNA Mutational Analysis; D004268:DNA-Binding Proteins / Q000235:genetics*; D003920:Diabetes Mellitus / Q000235:genetics*; D006720:Homozygote; D006801:Humans; D007231:Infant, Newborn; D007232:Infant, Newborn, Diseases / Q000235:genetics*; D008297:Male; D009154:Mutation; D016133:Polymerase Chain Reaction; D012097:Repressor Proteins; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000235:genetics*; D014421:Turkey
chemical_list: D004268:DNA-Binding Proteins; D012097:Repressor Proteins; D014157:Transcription Factors; C531063:ZFP57 protein, human