title: Improved performance of Yarrowia lipolytica lipase-catalyzed kinetic resolution of (R,S)-2-octanol by an integrated strategy of interfacial activation, bioimprinting and immobilization.
abstract: Yarrowia lipolytica lipase (YLL) demonstrated an (R)-enantiopreference for efficient resolution of (R,S)-2-octanol. The activity, enantioselectivity, the ratio of substrate to enzyme, acetaldehyde tolerance, and operational stability of YLL were improved by an integrated strategy of interfacial activation, bioimprinting, and immobilization. In comparison with the control, both the enzymatic activity and enantioselectivity increased by a factor of 8.85 and 2.75 by the integrated strategy, respectively. Fifty-one percentage of conversion with 220 of enantioselectivity was obtained using the immobilized YLL prepared by the integrated strategy at a ratio of 104 of substrate to enzyme loaded. The immobilized YLL retained 97% of its initial activity without a decrease in enantioselectivity after 10 successive reuse cycles. Together these results will result in a promising strategy with the YYL for efficient resolution of (R,S)-2-octanol in practice.
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Chen
authors.initials: C
authors.lastname: Guo
authors.forename: Xi-Tong
authors.initials: XT
authors.lastname: Sun
authors.forename: Chun-Zhao
authors.initials: CZ
authors.lastname: Liu
mesh_terms: D055162:Biocatalysis; D004800:Enzymes, Immobilized / Q000378:metabolism*; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D000442:Octanols / Q000378:metabolism*; D013237:Stereoisomerism; D013379:Substrate Specificity; D025062:Yarrowia / Q000201:enzymology*
chemical_list: D004800:Enzymes, Immobilized; D000442:Octanols; C067943:2-octanol; D008049:Lipase
keywords: 2-Octanol; Bioimprinting; Immobilization; Resolution; Yarrowia lipolytica lipase