title: Trans fat supplementation increases UV-radiation-induced oxidative damage on skin of mice.
abstract: We evaluated the influence of fish oil (FO, rich in n-3 FA), soybean oil (SO, rich in n-6 FA) and hydrogenated vegetable fat (HVF, rich in trans FA) on the oxidative status and viability of skin cells of mice exposed to ultraviolet radiation (UVR). Mice were supplemented with FO, SO or HVF for three months and exposed to UVR (2.72 mJ/cm(2)) for 2 days. One day after the last UVR session, the FO group showed higher levels of n-3 fatty acids (FA), while the HVF showed higher incorporation of trans FA (TFA) in dorsal skin. UVR increased lipid peroxidation and protein carbonyl levels of the HVF and to a lesser extent of the control and SO groups. Although all irradiated groups showed increased skin thickness, this increase was slighter in FO mice. UVR exposure reduced skin cell viability of the control, SO and HVF groups, while FO prevented this. Catalase activity was reduced independently of the supplementation and SOD level was increased in C and FO groups after UVR exposure; FO prevented the UVR-induced increase in glutathione levels, which was observed in skin of the control, SO and HVF mice. Our results showed the beneficial effects of FO supplementation, as well as the harmful effects of trans FA, whose intensity can increase vulnerability to skin diseases.
authors.affiliation: Programa de Pós-Graduação Em Farmacologia, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: R C S
authors.initials: RC
authors.lastname: Barcelos
authors.forename: H J
authors.initials: HJ
authors.lastname: Segat
authors.forename: D M
authors.initials: DM
authors.lastname: Benvegnú
authors.forename: F
authors.initials: F
authors.lastname: Trevizol
authors.forename: K
authors.initials: K
authors.lastname: Roversi
authors.forename: G S
authors.initials: GS
authors.lastname: Dolci
authors.forename: V T
authors.initials: VT
authors.lastname: Dias
authors.forename: J
authors.initials: J
authors.lastname: Piccolo
authors.forename: J C
authors.initials: JC
authors.lastname: Veit
authors.forename: T
authors.initials: T
authors.lastname: Emanuelli
authors.forename: M E
authors.initials: ME
authors.lastname: Bürger
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D004032:Diet; D005395:Fish Oils / Q000494:pharmacology; D008297:Male; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects* / Q000528:radiation effects; D012867:Skin / Q000187:drug effects* / Q000528:radiation effects; D013024:Soybean Oil / Q000494:pharmacology; D044242:Trans Fatty Acids / Q000494:pharmacology*; D014466:Ultraviolet Rays
chemical_list: D005395:Fish Oils; D044242:Trans Fatty Acids; D013024:Soybean Oil