title: Comparative performance of Thin Layer Agar and Löwenstein-Jensen culture for diagnosis of tuberculosis.
abstract: Sputum smear microscopy for the diagnosis of tuberculosis (TB) is cheap and simple but its sensitivity is low. Culture on Löwenstein-Jensen (LJ) is more sensitive but it takes a long time to yield results. Thin-Layer Agar (TLA) culture was suggested as an equally sensitive and faster alternative. We evaluated the performance of TLA for diagnosing TB in Jogjakarta, Indonesia. People with suspected TB presenting from July 2010 to July 2011 to two chest clinics of the National TB Control Programme network of Jogjakarta were eligible for inclusion. A sputum sample was sent to the Gadjah Mada University microbiology laboratory for concentration, smearing, Ziehl-Neelsen staining and culture on LJ and TLA. Sensitivity of cultures was evaluated against a composite reference standard (any positive culture). Time to detection of Mycobacteria was recorded. Out of 1414 samples, 164 (12%) were smear positive, 99 (7%) were scanty and 1151 (81%) were negative. On TLA and LJ respectively, 168 (12%) and 149 (11%) samples were positive, 72 (5%) and 32 (2%) were contaminated (κ = 0.64; 95% CI 0.59-0.69, p <0.01). Using the reference standard, 196 (14%) TB cases were identified. The sensitivity of TLA was 0.86 (95% CI 0.80-0.90), significantly higher (p 0.03) than for LJ (0.76; 95% CI 0.69-0.81). The median time to detection in days was significantly shorter (p <0.01) for TLA (12; 95% CI 11-13) than for LJ (44; 95% CI 43-45). TLA is a rapid and sensitive method for the diagnosis of TB. Implementation studies to evaluate the cost-effectiveness and impact of its introduction into programmatic settings are urgently needed.
authors.affiliation: Unit of Epidemiology and Disease Control, Department of Public Health, Institute of Tropical Medicine, Antwerp, Belgium.
authors.forename: T
authors.initials: T
authors.lastname: Battaglioli
authors.forename: N
authors.initials: N
authors.lastname: Rintiswati
authors.forename: A
authors.initials: A
authors.lastname: Martin
authors.forename: K R
authors.initials: KR
authors.lastname: Palupi
authors.forename: G
authors.initials: G
authors.lastname: Bernaerts
authors.forename: B
authors.initials: B
authors.lastname: Dwihardiani
authors.forename: R A
authors.initials: RA
authors.lastname: Ahmad
authors.forename: F
authors.initials: F
authors.lastname: Matthys
authors.forename: Y
authors.initials: Y
authors.lastname: Mahendradhata
authors.forename: P
authors.initials: P
authors.lastname: Van der Stuyft
mesh_terms: D000328:Adult; D000362:Agar; D000368:Aged; D000369:Aged, 80 and over; D003470:Culture Media / Q000737:chemistry*; D005260:Female; D006801:Humans; D007214:Indonesia; D008297:Male; D008875:Middle Aged; D009161:Mycobacterium / Q000302:isolation & purification*; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D013997:Time Factors; D014376:Tuberculosis / Q000175:diagnosis*; D055815:Young Adult
chemical_list: D003470:Culture Media; D000362:Agar
keywords: Culture; Thin Layer Agar; rapid detection; sensitivity; tuberculosis