title: CCL21 as an independent favorable prognostic factor for stage III/IV colorectal cancer.
abstract: The aim of the present study was to investigate the expression dynamics of CCL21 and its prognostic signiﬁcance in human stage III/IV colorectal cancer (CRC). CCL21 expression dynamics were detected with western blotting. The expression of CCL21 in CRC tissue microarrays was examined by immunohistochemistry. The optimal cut-point of CCL21 expression was assessed by the X-tile program. The prognostic significance was analyzed using both Kaplan-Meier curves and Cox regression analysis. Western blot analysis demonstrated that CCL21 expression was comparable in the CRC and normal colorectal tissues. According to the X-tile program, the cut-point for high expression of CCL21 in CRC was determined when the CCL21 expression index was >56.1. Overexpression of CCL21 was signiﬁcantly correlated with larger tumor diameter, more mucinous carcinoma or signet ring cell carcinoma and poor tumor differentiation. Patients with high expression of CCL21 had a higher overall survival rate in comparison to patients with low expression. In the multivariate Cox regression analysis, CCL21 expression was found to be an independent prognostic biomarker for CRC. ROC curves showed that CCL21 expression could improve the prognostic capability of TNM stage in stage III/IV CRC patients. High expression of CCL21 is an independent and useful biomarker for predicting longer survival of stage III/IV CRC patients.
authors.affiliation: Department of Colorectal Surgery, The Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, PR China.
authors.forename: Yifeng
authors.initials: Y
authors.lastname: Zou
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Chen
authors.forename: Xianrui
authors.initials: X
authors.lastname: Wu
authors.forename: Ruixue
authors.initials: R
authors.lastname: Yuan
authors.forename: Zerong
authors.initials: Z
authors.lastname: Cai
authors.forename: Xiaosheng
authors.initials: X
authors.lastname: He
authors.forename: Xinjuan
authors.initials: X
authors.lastname: Fan
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Wu
authors.forename: Ping
authors.initials: P
authors.lastname: Lan
mesh_terms: D002288:Adenocarcinoma, Mucinous / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics; D018279:Carcinoma, Signet Ring Cell / Q000235:genetics / Q000473:pathology; D002454:Cell Differentiation / Q000502:physiology; D054421:Chemokine CCL21 / Q000096:biosynthesis* / Q000235:genetics; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D015996:Survival Rate
chemical_list: D014408:Biomarkers, Tumor; C515478:CCL21 protein, human; D054421:Chemokine CCL21