title: Microarray analysis of two single-nucleotide polymorphisms of transforming growth factor alpha in patients with nonsyndromic cleft of north china.
abstract: OBJECTIVE
  To investigate the association between the TGFA:c.3851T > C (rs11466285) and TGFA:c.3822G > A (rs3771523) single-nucleotide polymorphisms (SNPs) and nonsyndromic cleft lip and/or cleft palate (CL/P) with microarray in north China.
  
  
  METHODS
  Test association by both case-control and case-parent analysis.
  
  
  METHODS
  Two SNPs of 150 controls, 166 cases, and 271 of their parents were genotyped using microarray based on the allele-specific primer extension, and chi-square statistics and family-based association test analyses were performed.
  
  
  RESULTS
  Both sequencing and microarray analysis produced identical results. We found significant evidence of overtransmission of the C allele of c.3851T > C and the A allele of c.3822G > A in case-parent trios for CL/P but not for cleft palate only (CP). Significant differences for both genotypic and allelic distributions between cases and controls were found at c.3822G > A and c.3851T > C for CL/P but not for CP. The TGFA [C; G] and [T; A] haplotypes showed significant overtransmission.
  
  
  CONCLUSIONS
  These results support that two SNPs are associated with nonsyndromic CL/P but not for CP in northern Chinese populations. It was demonstrated that this microarray is suitable to test SNPs associated with nonsyndromic CL/P.
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
authors.forename: Weitian
authors.initials: W
authors.lastname: Han
authors.forename: Yongping
authors.initials: Y
authors.lastname: Lu
authors.forename: Wang
authors.initials: W
authors.lastname: Yao
authors.forename: Zengjian
authors.initials: Z
authors.lastname: Li
authors.forename: Kai
authors.initials: K
authors.lastname: Fang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liu
authors.forename: Jianxin
authors.initials: J
authors.lastname: Li
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D002681:China; D002971:Cleft Lip / Q000235:genetics*; D002972:Cleft Palate / Q000235:genetics*; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D046228:Microarray Analysis; D020641:Polymorphism, Single Nucleotide; D016211:Transforming Growth Factor alpha / Q000235:genetics*
chemical_list: D016211:Transforming Growth Factor alpha
keywords: TGFA; microarray; nonsyndromic cleft lip and/or cleft palate; single-nucleotide polymorphism