title: Protein labeling with fluorogenic probes for no-wash live-cell imaging of proteins.
abstract: Protein labeling by using a protein tag and its specific fluorescent probe is increasingly becoming a useful technique for the real-time imaging of proteins in living cells. Recently, fluorogenic probes for protein labeling were developed. When using these probes, a washing step is not required for the removal of free probes from the cells, thus, allowing rapid detection of proteins in living cells with high signal-to-noise ratio. Various chemical principles have been applied in the designing of probes to include a turn-on fluorescence switch that is activated by the protein labeling reaction. In this review, we describe about the design strategy of the probes and the advances in fluorogenic protein labeling systems.
authors.affiliation: Division of Advanced Science and Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Hori
authors.forename: Kazuya
authors.initials: K
authors.lastname: Kikuchi
mesh_terms: D000906:Antibodies / Q000276:immunology; D002470:Cell Survival; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D057054:Molecular Imaging / Q000379:methods*; D011506:Proteins / Q000032:analysis*
chemical_list: D000906:Antibodies; D005456:Fluorescent Dyes; D011506:Proteins