title: Characterization of Edenia gomezpompae isolated from a patient with keratitis.
abstract: Edenia gomezpompae, isolated from a patient suffering from keratitis, who was an agricultural worker with a 4-year history of diabetes mellitus, is described. Fungal hyphae were detected in corneal scrapings after microscopic examination and repeated culture. Isolates were identified by morphology and by sequencing the internal transcribed spacer region of ribosomal DNA. Chlamydospore characteristics of E. gomezpompae are described for the first time. The in vitro antifungal susceptibilities of the isolate were tested using five antifungal agents. With the exception of fluconazole, the other drugs (natamycin, amphotericin B, itraconazole, and voriconazole) assayed were highly effective against this fungus. The keratitis was cured after debridement of the corneal ulcer and treatment with 5 % natamycin. After 3 months, when the patient was reexamined, the corneal ulcer showed signs of healing, with no recurrence. To our knowledge, this is the first reported case of keratitis caused by E. gomezpompae, as well as being the first known case of disease in humans caused by this species.
authors.affiliation: Department of Pathogenobiology, Jilin University Mycology Research Center, Norman Bethune College of Medicine, Jilin University, Changchun, 130021, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Cui
authors.forename: Hui
authors.initials: H
authors.lastname: Jia
authors.forename: Dan
authors.initials: D
authors.lastname: He
authors.forename: Huimei
authors.initials: H
authors.lastname: Yu
authors.forename: Song
authors.initials: S
authors.lastname: Gao
authors.forename: Koji
authors.initials: K
authors.lastname: Yokoyama
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.forename: Li
authors.initials: L
authors.lastname: Wang
mesh_terms: D000383:Agriculture; D000935:Antifungal Agents / Q000494:pharmacology / Q000627:therapeutic use; D001203:Ascomycota / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D003646:Debridement; D048909:Diabetes Complications; D005260:Female; D006801:Humans; D007634:Keratitis / Q000188:drug therapy / Q000382:microbiology* / Q000473:pathology* / Q000601:surgery; D008826:Microbial Sensitivity Tests; D008828:Microbiological Techniques; D008853:Microscopy; D008875:Middle Aged; D008969:Molecular Sequence Data; D009181:Mycoses / Q000175:diagnosis* / Q000188:drug therapy / Q000473:pathology* / Q000601:surgery; D017422:Sequence Analysis, DNA
chemical_list: D000935:Antifungal Agents; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer