title: Detection of Corchorus golden mosaic virus Associated with Yellow Mosaic Disease of Jute (Corchorus capsularis).
abstract: Yellow mosaic disease, caused by a whitefly transmitted New World Begomovirus, named Corchorus golden mosaic virus (CoGMV), is emerging as a serious biotic constraint for jute fibre production in Asia. For rapid and sensitive diagnosis of the Begomovirus associated with this disease, a non-radiolabelled diagnostic probe, developed against the DNA A component of the east Indian isolate of CoGMV, detected the presence of the virus in infected plants and viruliferous whiteflies following Southern hybridization and nucleic acid spot hybridization tests. Presence of the virus was also confirmed when polymerase chain reaction amplification was performed using virus-specific primers on DNA templates isolated from infected plants and viruliferous whiteflies.
authors.affiliation: Central Research Institute for Jute and Allied Fibres, Barrackpore, Kolkata, 700120 India ; International Crops Research Institute for the Semi Arid Tropics, Pathancheru, Hyderabad, 502324 India.
authors.forename: Raju
authors.initials: R
authors.lastname: Ghosh
authors.forename: Paramita
authors.initials: P
authors.lastname: Palit
authors.forename: Sujay
authors.initials: S
authors.lastname: Paul
authors.forename: Subrata Kumar
authors.initials: SK
authors.lastname: Ghosh
authors.forename: Anirban
authors.initials: A
authors.lastname: Roy
keywords: Begomovirus; Hybridization; New world; PCR; Probe