title: Fungus-specific sirtuin HstD coordinates secondary metabolism and development through control of LaeA.
abstract: The sirtuins are members of the NAD(+)-dependent histone deacetylase family that contribute to various cellular functions that affect aging, disease, and cancer development in metazoans. However, the physiological roles of the fungus-specific sirtuin family are still poorly understood. Here, we determined a novel function of the fungus-specific sirtuin HstD/Aspergillus oryzae Hst4 (AoHst4), which is a homolog of Hst4 in A. oryzae yeast. The deletion of all histone deacetylases in A. oryzae demonstrated that the fungus-specific sirtuin HstD/AoHst4 is required for the coordination of fungal development and secondary metabolite production. We also show that the expression of the laeA gene, which is the most studied fungus-specific coordinator for the regulation of secondary metabolism and fungal development, was induced in a ΔhstD strain. Genetic interaction analysis of hstD/Aohst4 and laeA clearly indicated that HstD/AoHst4 works upstream of LaeA to coordinate secondary metabolism and fungal development. The hstD/Aohst4 and laeA genes are fungus specific but conserved in the vast family of filamentous fungi. Thus, we conclude that the fungus-specific sirtuin HstD/AoHst4 coordinates fungal development and secondary metabolism via the regulation of LaeA in filamentous fungi.
authors.affiliation: Department of Molecular Biotechnology, Graduate School of Advanced Science of Matter, Hiroshima University, Kagamiyama, Higashi-Hiroshima, Hiroshima, Japan.
authors.forename: Moriyuki
authors.initials: M
authors.lastname: Kawauchi
authors.forename: Mika
authors.initials: M
authors.lastname: Nishiura
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Iwashita
mesh_terms: D001233:Aspergillus nidulans / Q000235:genetics / Q000378:metabolism; D001236:Aspergillus oryzae / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D006655:Histone Deacetylases / Q000145:classification / Q000235:genetics / Q000378:metabolism; D006801:Humans; D010802:Phylogeny; D037761:Sirtuins / Q000145:classification / Q000235:genetics / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D037761:Sirtuins; D006655:Histone Deacetylases