title: A simple and effective pressure culture system modified from a transwell cell culture system.
abstract: Mechanical pressure plays an important role in many physiological and pathological processes. Mimicking the mechanical pressure present in vitro is necessary for related research, but usually requires expensive and complicated equipment. In this study we created a simple pressure culture system based on the transwell culture system. By cutting off the top rim of the transwell insert, the cells were compressed between the insert membrane and the well floor. The new pressure culture system was proven effective in that it induced cell morphological change, integrin β1 upregulation, actin polymerization and growth change in rat retinal ganglion cells, human nasopharyngeal carcinoma cells and mice embryonic fibroblasts. Though the pressure value is immeasurable and inhomogeneous, the easily available culture system still provides a choice for the laboratories that do not have access to the better, but much more expensive pressure culture equipment.
authors.affiliation: Institute of Aging Research, Key Laboratory for Medical Molecular Diagnostics of Guangdong Province, Guangdong Medical College, Dongguan 523808, China.
authors.forename: Xuerong
authors.initials: X
authors.lastname: Sun
authors.forename: Xinguang
authors.initials: X
authors.lastname: Liu
authors.forename: Yuehong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xielan
authors.initials: X
authors.lastname: Kuang
authors.forename: Bo
authors.initials: B
authors.lastname: Lv
authors.forename: Jian
authors.initials: J
authors.lastname: Ge
mesh_terms: D008841:Actin Cytoskeleton / Q000502:physiology; D000704:Analysis of Variance; D000818:Animals; D002277:Carcinoma; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line / Q000502:physiology; D049109:Cell Proliferation; D005347:Fibroblasts / Q000502:physiology; D005455:Fluorescent Antibody Technique / Q000379:methods; D006801:Humans; D006874:Hydrostatic Pressure; D019012:Integrin beta1 / Q000235:genetics*; D008744:Methylamines; D008810:Mice, Inbred C57BL; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000473:pathology; D061251:Primary Cell Culture; D051381:Rats; D060888:Real-Time Polymerase Chain Reaction; D012165:Retinal Ganglion Cells / Q000166:cytology / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D013314:Stress, Mechanical
chemical_list: C422026:1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarboxyamine perchlorate; D019012:Integrin beta1; D008744:Methylamines