title: Gossypetin, a naturally occurring hexahydroxy flavone, ameliorates gamma radiation-mediated DNA damage.
abstract: OBJECTIVE
  To evaluate the protective effect of gossypetin (GTIN) against gamma (γ)-radiation-mediated DNA damage.
  
  
  METHODS
  Increasing concentrations (10-150 μM) of GTIN were incubated with supercoiled DNA 1 h prior exposure to γ-radiation in the range of 5-Gy absorbed dose from Co(60) γ source. To establish the effective protective concentration of GTIN, supercoiled DNA was pre-incubated with 50 μM of GTIN for 1 h followed by exposure of 5, 10 and 20 Gy doses of γ-radiation. Moreover, 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical, hydroxyl radical, nitric oxide (NO) scavenging, metal chelating activity and ferric reducing antioxidant power (FRAP) of GTIN were measured and compared with standards. The flowcytometric analysis and radiation-induced genomic DNA damage by comet assay were employed to estimate the level of intracellular reactive oxygen species (ROS) using isolated murine hepatocytes.
  
  
  RESULTS
  GTIN was able to effectively scavenge different free radicals in in vitro situations. It could significantly prevent radiation induced supercoiled and genomic DNA damage with reduced comet parameters. It also acted as a potent scavenger of the radiation induced ROS.
  
  
  CONCLUSIONS
  GTIN ameliorated radiation-induced oxidative stress and DNA damage by its free-radical scavenging activity.
authors.affiliation: Department of Physiology, UCSTA, University of Calcutta , Kolkata.
authors.forename: Amitava
authors.initials: A
authors.lastname: Khan
authors.forename: Krishnendu
authors.initials: K
authors.lastname: Manna
authors.forename: Chinchu
authors.initials: C
authors.lastname: Bose
authors.forename: Mahuya
authors.initials: M
authors.lastname: Sinha
authors.forename: Dipesh Kr
authors.initials: DK
authors.lastname: Das
authors.forename: Swaraj Bandhu
authors.initials: SB
authors.lastname: Kesh
authors.forename: Anindita
authors.initials: A
authors.lastname: Chakrabarty
authors.forename: Asoke
authors.initials: A
authors.lastname: Banerji
authors.forename: Sanjit
authors.initials: S
authors.lastname: Dey
mesh_terms: D000818:Animals; D001688:Biological Products / Q000378:metabolism / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000378:metabolism; D004249:DNA Damage; D004278:DNA, Superhelical / Q000235:genetics; D005419:Flavonoids / Q000378:metabolism / Q000494:pharmacology*; D016166:Free Radical Scavengers / Q000378:metabolism / Q000494:pharmacology*; D005720:Gamma Rays / Q000009:adverse effects*; D017665:Hydroxyl Radical / Q000378:metabolism; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D007502:Iron Chelating Agents / Q000378:metabolism / Q000494:pharmacology*; D008297:Male; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects / Q000528:radiation effects; D010851:Picrates / Q000378:metabolism; D011837:Radiation-Protective Agents / Q000378:metabolism / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D001688:Biological Products; D001713:Biphenyl Compounds; D004278:DNA, Superhelical; D005419:Flavonoids; D016166:Free Radical Scavengers; D007502:Iron Chelating Agents; D010851:Picrates; D011837:Radiation-Protective Agents; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl; C059922:gossypetin