title: Protein O glycosylation regulates activation of hepatic stellate cells.
abstract: It was reported that O glycosylation is associated with hepatic stellate cell activation and regulates collagen expression. In this study, we aimed to investigate the effect of O glycosylation on the activation of human hepatic stellate cells. We found that the inhibitor of O glycosylation, benzyl-α-GalNAc (2 and 4 mM), could significantly inhibit cells proliferation in a dose-dependent manner. Moreover, benzyl-α-GalNAc decreased the expressions of α-smooth muscle actin, collagen I, and collagen III. The results indicate that O glycosylation is involved in the activation of hepatic stellate cells.
authors.affiliation: Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing, 100015, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Fan
authors.forename: Song
authors.initials: S
authors.lastname: Chuan
authors.forename: Wei
authors.initials: W
authors.lastname: Hongshan
mesh_terms: D000116:Acetylgalactosamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D000199:Actins / Q000096:biosynthesis; D001593:Benzyl Compounds / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D024042:Collagen Type I / Q000096:biosynthesis; D024061:Collagen Type III / Q000096:biosynthesis; D015870:Gene Expression / Q000187:drug effects; D006031:Glycosylation / Q000187:drug effects*; D055166:Hepatic Stellate Cells / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D008099:Liver / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*
chemical_list: C541116:ACTA2 protein, human; D000199:Actins; D001593:Benzyl Compounds; D024042:Collagen Type I; D024061:Collagen Type III; C080739:benzyl-alpha-N-acetylgalactosamine; D000116:Acetylgalactosamine