title: Current trends in detecting non-O157 Shiga toxin-producing Escherichia coli in food.
abstract: Non-O157 Shiga toxin-producing Escherichia coli (non-O157 STEC) strains are increasingly recognized as important foodborne pathogens worldwide. Together with E. coli O157:H7, six additional STEC serogroups (O26, O45, O103, O111, O121, and O145) are now regulated as adulterants in certain raw beef products in the United States. However, effective detection and isolation of non-O157 STEC strains from food matrices remain challenging. In the past decade, great attention has been paid to developing rapid and reliable detection methods for STEC in general (targeting common virulence factors) and specific STEC serogroups in particular (targeting serogroup-specific traits). This review summarizes current trends in detecting non-O157 STEC in food, including culture, immunological, and molecular methods, as well as several novel technologies.
authors.affiliation: Department of Food Science, Louisiana State University Agricultural Center , Baton Rouge, Louisiana, USA.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.forename: Qianru
authors.initials: Q
authors.lastname: Yang
authors.forename: Julie A
authors.initials: JA
authors.lastname: Kase
authors.forename: Jianghong
authors.initials: J
authors.lastname: Meng
authors.forename: Laurie M
authors.initials: LM
authors.lastname: Clotilde
authors.forename: Andrew
authors.initials: A
authors.lastname: Lin
authors.forename: Beilei
authors.initials: B
authors.lastname: Ge
mesh_terms: D000818:Animals; D002417:Cattle; D015169:Colony Count, Microbial; D019453:Escherichia coli O157 / Q000254:growth & development / Q000302:isolation & purification; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology / Q000379:methods* / Q000639:trends*; D008461:Meat Products / Q000382:microbiology*; D012703:Serotyping; D054323:Shiga-Toxigenic Escherichia coli / Q000145:classification / Q000254:growth & development / Q000302:isolation & purification*; D014481:United States; D037521:Virulence Factors
chemical_list: D037521:Virulence Factors