title: Memb-roller: an effective way of making membrane rolls for pathological examination and studies of human placenta.
abstract: For the pathological evaluation of the chorioamniotic membranes, a membrane roll is made to give an orientation to the sections and to cover more extended planes. We designed a new instrument, Annie's memb-roller, for easy preparation of membrane rolls. Annie's memb-roller has two non-toothed arms which are 2 mm wide, 1 mm thick, and 8 cm long. To test its effectiveness in making a membrane roll, the placentas obtained from pregnant women (n = 10) with various obstetrical conditions were used. Preparation of the membrane rolls using Annie's memb-roller was easy and successful in all cases tested. The lengths of the membranes measured from fresh placentas and paraffin sections showed a very good correlation (p < 0.001; r = 0.941). Application of the memb-roller would be very helpful in the semi-quantitative analysis of the chorioamniotic membranes and the standardization of placental pathology and research practice.
authors.affiliation: Asan Laboratory of Perinatal Science, Asan Institute for Life Sciences, Asan Medical Center, Seoul, Republic of Korea.
authors.forename: Y W
authors.initials: YW
authors.lastname: Kim
authors.forename: J-S
authors.initials: JS
authors.lastname: Kim
authors.forename: K A
authors.initials: KA
authors.lastname: Lee
authors.forename: J-Y
authors.initials: JY
authors.lastname: Shim
authors.forename: H-S
authors.initials: HS
authors.lastname: Won
authors.forename: P R
authors.initials: PR
authors.lastname: Lee
authors.forename: A
authors.initials: A
authors.lastname: Kim
authors.forename: C J
authors.initials: CJ
authors.lastname: Kim
mesh_terms: D000328:Adult; D049033:Chorioallantoic Membrane / Q000473:pathology*; D004867:Equipment Design; D005260:Female; D006801:Humans; D010338:Pathology, Clinical / Q000295:instrumentation* / Q000379:methods; D010920:Placenta / Q000473:pathology*; D011247:Pregnancy