title: Creating protein-imprinted self-assembled monolayers with multiple binding sites and biocompatible imprinted cavities.
abstract: Imprinted monolayers have several advantages over bulk imprinted polymers such as excellent mass transfer of molecules into and out of imprinted sites and transduction of binding signals detected in real time. Protein-imprinted self-assembled monolayers (SAMs) were created with multiple binding sites and biocompatible imprinted cavities from functional thiols and novel disulfide compounds containing an oligoethylene glycol (OEG) terminal moiety and two amide groups incorporated in the chain (DHAP) in a biologically benign solution. DHAP played an important role in the formation of multiple binding sites and biocompatible cavities in addition to resisting nonspecific protein binding. The created protein-imprinted SAMs exhibited the excellent ability of specific binding of target proteins determined by multiple binding sites and imprinted cavities. The strategy generates tailor-made monolayer surfaces with specific protein binding and opens the possibility of controlled assembly of intellectual biomaterials and preparation of biosensors.
authors.affiliation: Key Laboratory of Mesoscopic Chemistry (Ministry of Education), and School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, People's Republic of China.
authors.forename: Xianfeng
authors.initials: X
authors.lastname: Zhang
authors.forename: Xuezhong
authors.initials: X
authors.lastname: Du
authors.forename: Xuan
authors.initials: X
authors.lastname: Huang
authors.forename: Zhongpeng
authors.initials: Z
authors.lastname: Lv
mesh_terms: D000577:Amides / Q000737:chemistry; D001665:Binding Sites; D001672:Biocompatible Materials / Q000737:chemistry*; D004220:Disulfides / Q000737:chemistry; D008958:Models, Molecular; D054802:Molecular Imprinting; D015394:Molecular Structure; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry; D011506:Proteins / Q000138:chemical synthesis* / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: D000577:Amides; D001672:Biocompatible Materials; D004220:Disulfides; D011108:Polymers; D011506:Proteins; D013438:Sulfhydryl Compounds