title: Clinical significance of RECK promoter methylation in pancreatic ductal adenocarcinoma.
abstract: The aim of this study was to analyze the clinical significance of reversion-inducing-cysteine-rich protein with Kazal motifs (RECK) promoter methylation in pancreatic ductal adenocarcinoma (PDA). Methylation-specific polymerase chain reaction was used to examine the promoter methylation status of RECK in 60 pairs of PDA tissue samples and adjacent non-cancerous tissue samples. Statistical analyses were applied to test the associations between RECK promoter methylation status, clinicopathologic factors, and prognosis. The rate of RECK promoter methylation was significantly higher in PDA tissues than in adjacent non-cancerous tissues (P < 0.001). RECK methylation status was significantly associated with clinical stage (P = 0.017), histological differentiation (P = 0.046), and lymph node metastasis (P = 0.003), but was not associated with gender, age, and tumor location (all P > 0.05). Additionally, RECK promoter methylation is associated with malignant behavior and poor prognosis. In conclusion, determination of RECK promoter methylation status in tumor tissues may assist in the identification of patients who require aggressive postoperative intervention in order to improve prognosis.
authors.affiliation: Center of Organ Transplantation, Ruijin Hospital, School of Medicine, Shanghai Jiaotong University, No. 197, Ruijiner Road, Shanghai, 200025, China.
authors.forename: Xiong-Xiong
authors.initials: XX
authors.lastname: Lu
authors.forename: Shu-Min
authors.initials: SM
authors.lastname: Zhang
authors.forename: Yuan
authors.initials: Y
authors.lastname: Fang
authors.forename: Zheng-Ting
authors.initials: ZT
authors.lastname: Wang
authors.forename: Jun-Jie
authors.initials: JJ
authors.lastname: Xie
authors.forename: Qian
authors.initials: Q
authors.lastname: Zhan
authors.forename: Xia-Xing
authors.initials: XX
authors.lastname: Deng
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.forename: Jia-Bin
authors.initials: JB
authors.lastname: Jin
authors.forename: Cheng-Hong
authors.initials: CH
authors.lastname: Peng
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Li
authors.forename: Bai-Yong
authors.initials: BY
authors.lastname: Shen
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D021441:Carcinoma, Pancreatic Ductal / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D019175:DNA Methylation; D005260:Female; D058851:GPI-Linked Proteins / Q000235:genetics*; D006801:Humans; D053208:Kaplan-Meier Estimate; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009367:Neoplasm Staging; D010190:Pancreatic Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D011379:Prognosis; D011401:Promoter Regions, Genetic / Q000235:genetics*; D016896:Treatment Outcome
chemical_list: D058851:GPI-Linked Proteins; C115460:RECK protein, human