title: Efficient production of polymer-grade D-lactate by Sporolactobacillus laevolacticus DSM442 with agricultural waste cottonseed as the sole nitrogen source.
abstract: In this study, we show that Sporolactobacillus laevolacticus DSM442 can produce d-lactate by using cottonseed as the sole nitrogen source. The cottonseed was enzymatically hydrolyzed and simultaneously utilized during d-lactate fermentation. Under optimal conditions, a high d-lactic acid concentration (144.4 g/L) was obtained in a fed-batch fermentation within 35 h, with an average productivity of 4.13 g/(Lh) and a yield of 0.96 g/g glucose. The optical purity of d-lactic acid in the broth was 99.3%, which meets the requirement for use in lactic acid polymerization. Our study represents a cost-effective method for polymer-grade d-lactate production using cheap agricultural wastes.
authors.affiliation: Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.forename: Limin
authors.initials: L
authors.lastname: Wang
authors.forename: Jiansong
authors.initials: J
authors.lastname: Ju
authors.forename: Bo
authors.initials: B
authors.lastname: Yu
authors.forename: Yanhe
authors.initials: Y
authors.lastname: Ma
mesh_terms: D032561:Cotton Fiber; D005947:Glucose / Q000032:analysis; D019344:Lactic Acid / Q000737:chemistry*; D007778:Lactobacillus / Q000378:metabolism*; D009584:Nitrogen / Q000737:chemistry*; D013696:Temperature
chemical_list: D019344:Lactic Acid; D005947:Glucose; D009584:Nitrogen
keywords: Cottonseed; Polymer-grade; Sporolactobacillus laevolacticus; d-Lactate