title: Spectroscopic studies on the interaction of carteolol hydrochloride and urea-induced bovine serum albumin.
abstract: The interaction of carteolol hydrochloride, to 0.2 mol L(-1) urea-induced bovine serum albumin in aqueous solution has been first investigated by fluorescence spectra and ultraviolet-visible (UV-vis) spectra at pH 7.40. The quenching mechanism, binding parameter and sites (n), the binding mode (ΔG, ΔH, and ΔS) as well as the binding distance (r) have been obtained according to the experimental results. We also use the synchronous fluorescence method to study the effect of CTL on the conformation change of urea-induced BSA.
authors.affiliation: Key Laboratory of Theoretical Chemistry and Molecular Simulation of Ministry of Education, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan 411201, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Yao
authors.forename: Xianyong
authors.initials: X
authors.lastname: Yu
authors.forename: Tongyin
authors.initials: T
authors.lastname: Zheng
authors.forename: Heting
authors.initials: H
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.forename: Pinggui
authors.initials: P
authors.lastname: Yi
mesh_terms: D000818:Animals; D001665:Binding Sites; D002354:Carteolol / Q000737:chemistry / Q000378:metabolism*; D002417:Cattle; D004735:Energy Transfer / Q000187:drug effects; D007700:Kinetics; D008968:Molecular Conformation; D011485:Protein Binding / Q000187:drug effects; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013696:Temperature; D014508:Urea / Q000494:pharmacology*
chemical_list: D012710:Serum Albumin, Bovine; D002354:Carteolol; D014508:Urea
keywords: Carteolol hydrochloride; Fluorescence spectroscopy; Interaction; UV–visible spectroscopy; Urea-induced bovine serum albumin