title: Translational halt during elongation caused by G-quadruplex formed by mRNA.
abstract: mRNA forms various secondary and tertiary structures that affect gene expression. Although structures formed in the untranslated regions (UTRs) of mRNAs that inhibit translation have been characterized, stable mRNA structures in open reading frames (ORFs) may also cause translational halt or slow translation elongation. We previously established a method, termed a synchronized translation assay, that enables time course analysis of single turnover translation elongation. In this method, translation initiation, which is a rate determining step of the translation procedure, can be ignored because all ribosomes are synchronized on a specific position of mRNA before translation elongation is restarted from this position. In this paper, we used the synchronized translation assay to evaluate the effects of a G-quadruplex structure located at various positions within the mRNA ORF on translational halt.
authors.affiliation: Frontier Institute for Biomolecular Engineering Research (FIBER), Konan University, Kobe, Japan.
authors.forename: Tamaki
authors.initials: T
authors.lastname: Endoh
authors.forename: Yu
authors.initials: Y
authors.lastname: Kawasaki
authors.forename: Naoki
authors.initials: N
authors.lastname: Sugimoto
mesh_terms: D054856:G-Quadruplexes; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010441:Peptide Chain Elongation, Translational; D014176:Protein Biosynthesis; D012333:RNA, Messenger / Q000737:chemistry*; D013997:Time Factors
chemical_list: D012333:RNA, Messenger
keywords: G-quadruplex; Kinetic analysis; Synchronized translation; Translation elongation; mRNA structure