title: Silk microgels formed by proteolytic enzyme activity.
abstract: The proteolytic enzyme α-chymotrypsin selectively cleaves the amorphous regions of silk fibroin protein (SFP) and allows the crystalline regions to self-assemble into silk microgels (SMGs) at physiological temperature. These microgels consist of lamellar crystals in the micrometer scale, in contrast to the nanometer-scaled crystals in native silkworm fibers. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and zeta potential results demonstrated that α-chymotrypsin utilized only the non-amorphous domains or segments of the heavy chain of SFP to form negatively charged SMGs. The SMGs were characterized in terms of size, charge, structure, morphology, crystallinity, swelling kinetics, water content and thermal properties. The results suggest that the present technique of preparing SMGs by α-chymotrypsin is simple and efficient, and that the prepared SMGs have useful features for studies related to biomaterial and pharmaceutical needs. This process is also an easy way to obtain the amorphous peptide chains for further study.
authors.affiliation: BioLab-UdR-INSTM, Via Vecchia Livornese, University of Pisa, Pisa 1291, Italy.
authors.forename: Sangram K
authors.initials: SK
authors.lastname: Samal
authors.forename: Mamoni
authors.initials: M
authors.lastname: Dash
authors.forename: Federica
authors.initials: F
authors.lastname: Chiellini
authors.forename: David L
authors.initials: DL
authors.lastname: Kaplan
authors.forename: Emo
authors.initials: E
authors.lastname: Chiellini
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis*; D002918:Chymotrypsin / Q000737:chemistry*; D005349:Fibroins / Q000138:chemical synthesis*; D005782:Gels / Q000138:chemical synthesis*; D008422:Materials Testing; D057139:Nanofibers / Q000032:analysis / Q000737:chemistry*; D059748:Proteolysis
chemical_list: D001672:Biocompatible Materials; D005782:Gels; C482288:fibroin, silkworm; D005349:Fibroins; D002918:Chymotrypsin; C117608:alpha-chymotrypsin
keywords: Microgels; Peptide; Self-assemble; Silk fibroin protein; α-Chymotrypsin