title: Biofunctionalization of multiwalled carbon nanotubes by irradiation of electropolymerized poly(pyrrole-diazirine) films.
abstract: A photoactivatable poly(pyrrole-diazirine) film was synthesized and electropolymerized as a versatile tool for covalent binding of laccase and glucose oxidase on multiwalled carbon nanotube coatings and Pt, respectively. Irradiation of the functionalized nanotubes allowed photochemical grafting of laccase and its subsequent direct electrical wiring, as illustrated by the electrocatalytic reduction of oxygen. Moreover, covalent binding of glucose oxidase as model enzyme, achieved by UV activation of electropolymerized pyrrole-diazirine, allowed a glucose biosensor to be realized. This original method to graft biomolecules combines electrochemical and photochemical techniques. The simplicity of this new method allows it to be extended easily to other biological systems.
authors.affiliation: NTU CREATE, Research Wing #02-06, Nanyang Technological University, 138602, Singapore.
authors.forename: Vladislav
authors.initials: V
authors.lastname: Papper
authors.forename: Karine
authors.initials: K
authors.lastname: Gorgy
authors.forename: Kamal
authors.initials: K
authors.lastname: Elouarzaki
authors.forename: Ayrine
authors.initials: A
authors.lastname: Sukharaharja
authors.forename: Serge
authors.initials: S
authors.lastname: Cosnier
authors.forename: Robert S
authors.initials: RS
authors.lastname: Marks
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D003978:Diazomethane / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000737:chemistry*; D004563:Electrochemistry / Q000379:methods; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D004800:Enzymes, Immobilized; D037742:Nanotubes, Carbon; D011108:Polymers; C586126:poly(pyrrole-diazirine); D003978:Diazomethane; D005949:Glucose Oxidase
keywords: electrochemistry; enzymes; immobilization; nanotubes; polymers