title: Purification of astrocytes from transgenic rodents by fluorescence-activated cell sorting.
abstract: The purification of astrocytes by fluorescence-activated cell sorting (FACS) requires that an astrocyte-specific promoter drive the expression of the green fluorescent protein (GFP). Our laboratory uses FACS to acutely isolate astrocytes from young and old tissue as well as to isolate GFP-negative neurons at the end of the FACS sorting to conduct comparative unbiased, large-scale gene expression studies. Because of the relatively harsh nature of FACS sorting, few astrocytes or neurons survive long enough after the sort to be cultured.
authors.affiliation: Department of Neurobiology, School of Medicine, Stanford University, Stanford, California 94305, USA. lynettefoo@gmail.com
authors.forename: Lynette C
authors.initials: LC
authors.lastname: Foo
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism* / Q000502:physiology; D002470:Cell Survival; D005434:Flow Cytometry / Q000379:methods*; D020869:Gene Expression Profiling / Q000379:methods; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D049452:Green Fluorescent Proteins