title: Ficolin-2 and ficolin-3 in women with malignant and benign ovarian tumours.
abstract: Ficolins are serum pattern recognition molecules. They have opsonic properties and are able to activate complement via the lectin pathway. This paper reports investigations concerning ficolin-2 and ficolin-3 in ovarian cancer (OC). Their serum levels, single nucleotide polymorphisms of the corresponding FCN2 and FCN3 genes and specific mRNA expression in ovarian sections were investigated in 128 patients suffering from primary OC and 197 controls operated on for reasons other than malignancies. The latter consisted of two reference groups: those with benign tumours (n = 123) and those with normal ovaries (NO) (n = 74). Serum ficolin-2 and ficolin-3 concentrations were higher among patients with malignant disease when compared with either of the reference groups. A significant correlation between ficolin-2 and ficolin-3 concentrations was found, while no correlations with CA125 antigen or CRP were observed. No differences in the frequency of single nucleotide polymorphisms at sites -64, -4 (promoter), +6359, or +6424 (exon 8) (FCN2 gene) nor in the frame-shift mutation 1637delC (FCN3 gene) were found between investigated groups. In contrast to serum concentrations, the expression of FCN2 gene (reported for the first time in ovarian sections) was significantly lower in women with OC in comparison with patients with NO but not with benign ovarian tumours. In case of FCN3 gene, its expression levels in OC group inversely correlated with serum ficolin-3 and were lower in comparison with controls.
authors.affiliation: Laboratory of Immunobiology of Infections, Institute of Medical Biology, Polish Academy of Sciences, Lodowa 106, 93-232, Lodz, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Szala
authors.forename: Sambor
authors.initials: S
authors.lastname: Sawicki
authors.forename: Anna St
authors.initials: AS
authors.lastname: Swierzko
authors.forename: Janusz
authors.initials: J
authors.lastname: Szemraj
authors.forename: Marcin
authors.initials: M
authors.lastname: Sniadecki
authors.forename: Mateusz
authors.initials: M
authors.lastname: Michalski
authors.forename: Andrzej
authors.initials: A
authors.lastname: Kaluzynski
authors.forename: Jolanta
authors.initials: J
authors.lastname: Lukasiewicz
authors.forename: Anna
authors.initials: A
authors.lastname: Maciejewska
authors.forename: Dariusz
authors.initials: D
authors.lastname: Wydra
authors.forename: David C
authors.initials: DC
authors.lastname: Kilpatrick
authors.forename: Misao
authors.initials: M
authors.lastname: Matsushita
authors.forename: Maciej
authors.initials: M
authors.lastname: Cedzynski
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005787:Gene Frequency; D005838:Genotype; D006023:Glycoproteins / Q000097:blood* / Q000235:genetics*; D006801:Humans; D037102:Lectins / Q000097:blood* / Q000235:genetics*; D008875:Middle Aged; D009367:Neoplasm Staging; D010051:Ovarian Neoplasms / Q000097:blood* / Q000235:genetics* / Q000473:pathology; D020641:Polymorphism, Single Nucleotide; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult; D000097604:Ficolins
chemical_list: D006023:Glycoproteins; D037102:Lectins; D000097604:Ficolins; C109662:FCN3 protein, human