title: Development of a novel FRET probe for the real-time determination of ceramidase activity.
abstract: Fretful novelty: We developed two novel doubly labelled fluorescent ceramide analogues that exhibit significant FRET and undergo hydrolysis by ceramidases. We present a fluorescent sphingolipid FRET probe that allows homogeneous ratiometric determination of enzyme activity in real-time.
authors.affiliation: Department of Organic & Bioorganic Chemistry, Humboldt Universität zu Berlin, Institute for Chemistry, Brook-Taylor Str-2, 12489 Berlin, Germany.
authors.forename: Krishna P
authors.initials: KP
authors.lastname: Bhabak
authors.forename: Anett
authors.initials: A
authors.lastname: Hauser
authors.forename: Susanne
authors.initials: S
authors.lastname: Redmer
authors.forename: Sebastian
authors.initials: S
authors.lastname: Banhart
authors.forename: Dagmar
authors.initials: D
authors.lastname: Heuer
authors.forename: Christoph
authors.initials: C
authors.lastname: Arenz
mesh_terms: D055572:Ceramidases / Q000378:metabolism*; D002518:Ceramides / Q000737:chemistry* / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D006056:Golgi Apparatus / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D006868:Hydrolysis; D008856:Microscopy, Fluorescence; D010069:Oxadiazoles / Q000737:chemistry; D010078:Oxazines / Q000737:chemistry; D054467:Phospholipases A2 / Q000378:metabolism
chemical_list: C526864:7-nitrobenz-2-oxa-1,3-diazole; D002518:Ceramides; D005456:Fluorescent Dyes; D010069:Oxadiazoles; D010078:Oxazines; D054467:Phospholipases A2; D055572:Ceramidases; C044808:nile red