title: Quantitative trait loci (QTL) associated with resistance to a monogenean parasite (Benedenia seriolae) in yellowtail (Seriola quinqueradiata) through genome wide analysis.
abstract: Benedenia infections caused by the monogenean fluke ectoparasite Benedenia seriolae seriously impact marine finfish aquaculture. Genetic variation has been inferred to play a significant role in determining the susceptibility to this parasitic disease. To evaluate the genetic basis of Benedenia disease resistance in yellowtail (Seriola quinqueradiata), a genome-wide and chromosome-wide linkage analyses were initiated using F1 yellowtail families (n = 90 per family) based on a high-density linkage map with 860 microsatellite and 142 single nucleotide polymorphism (SNP) markers. Two major quantitative trait loci (QTL) regions on linkage groups Squ2 (BDR-1) and Squ20 (BDR-2) were identified. These QTL regions explained 32.9-35.5% of the phenotypic variance. On the other hand, we investigated the relationship between QTL for susceptibility to B. seriolae and QTL for fish body size. The QTL related to growth was found on another linkage group (Squ7). As a result, this is the first genetic evidence that contributes to detailing phenotypic resistance to Benedenia disease, and the results will help resolve the mechanism of resistance to this important parasitic infection of yellowtail.
authors.affiliation: National Research Institute of Aquaculture, Fisheries Research Agency, Nakatsuhamaura, Minamiise-cho, Watarai-gun, Mie, Japan. aozaki@affrc.go.jp
authors.forename: Akiyuki
authors.initials: A
authors.lastname: Ozaki
authors.forename: Kazunori
authors.initials: K
authors.lastname: Yoshida
authors.forename: Kanako
authors.initials: K
authors.lastname: Fuji
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kubota
authors.forename: Wataru
authors.initials: W
authors.lastname: Kai
authors.forename: Jun-ya
authors.initials: JY
authors.lastname: Aoki
authors.forename: Yumi
authors.initials: Y
authors.lastname: Kawabata
authors.forename: Junpei
authors.initials: J
authors.lastname: Suzuki
authors.forename: Kazuki
authors.initials: K
authors.lastname: Akita
authors.forename: Takashi
authors.initials: T
authors.lastname: Koyama
authors.forename: Masahiro
authors.initials: M
authors.lastname: Nakagawa
authors.forename: Takurou
authors.initials: T
authors.lastname: Hotta
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Tsuzaki
authors.forename: Nobuaki
authors.initials: N
authors.lastname: Okamoto
authors.forename: Kazuo
authors.initials: K
authors.lastname: Araki
authors.forename: Takashi
authors.initials: T
authors.lastname: Sakamoto
mesh_terms: D000818:Animals; D049628:Body Size / Q000235:genetics; D002874:Chromosome Mapping; D002875:Chromosomes / Q000235:genetics; D060467:Disease Resistance / Q000235:genetics*; D005393:Fish Diseases / Q000469:parasitology*; D005399:Fishes / Q000235:genetics* / Q000254:growth & development / Q000469:parasitology* / Q000502:physiology; D023281:Genomics; D054884:Host-Pathogen Interactions / Q000235:genetics; D018895:Microsatellite Repeats / Q000235:genetics; D010641:Phenotype; D010986:Platyhelminths / Q000502:physiology*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D040641:Quantitative Trait Loci