title: Multilocus sequence analysis of Aspergillus Sect. Nigri in dried vine fruits of worldwide origin.
abstract: Dried vine fruits may be heavily colonized by Aspergillus species. The molecular biodiversity of an Aspergillus population (234 strains) isolated from dried vine fruit samples of worldwide origin were analyzed by investigating four housekeeping gene loci (calmodulin, β-tubulin, elongation factor 1-α, RPB2). Aspergillus Sect. Nigri was dominant and the strains were identified as A. tubingensis (138), A. awamori (38), A. carbonarius (27), A. uvarum (16) and A. niger (11). Four Aspergillus flavus strains were also identified from Chilean raisins. Two clusters closely related to the A. tubingensis species with a significant bootstrap (60% and 99%) were identified as distinct populations. Among the four loci, RPB2 showed the highest genetic variability. This is the first complete study on the worldwide distribution of black Aspergilli occurring on dried vine fruits identified by a molecular approach.
authors.affiliation: Institute of Sciences of Food Production, ISPA-CNR, Via Amendola 122/0, 70126, Bari, Italy.
authors.forename: Antonia
authors.initials: A
authors.lastname: Susca
authors.forename: Giancarlo
authors.initials: G
authors.lastname: Perrone
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Cozzi
authors.forename: Gaetano
authors.initials: G
authors.lastname: Stea
authors.forename: Antonio F
authors.initials: AF
authors.lastname: Logrieco
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Mulè
mesh_terms: D001230:Aspergillus / Q000235:genetics* / Q000302:isolation & purification; D044822:Biodiversity; D005516:Food Microbiology; D005638:Fruit / Q000382:microbiology*; D005800:Genes, Fungal / Q000235:genetics; D058885:Multilocus Sequence Typing; D010802:Phylogeny