title: Optical imaging for brain tissue characterization using relative fluorescence lifetime imaging.
abstract: An autofluorescence lifetime wide-field imaging system that can generate contrast in underlying tissue structures of normal and malignant brain tissue samples with video rate acquisition and processing time is presented. Images of the investigated tissues were acquired with high resolution (∼35 μm) using an algorithm to produce contrast based on differences in relative lifetimes. Sufficient contrast for delineation was produced without the computation of fluorescence decay times or Laguerre coefficients. The imaged tissues were sent for histological analysis that confirmed the detected imaged tissues morphological findings and correlations between relative lifetime maps and histology identified.
authors.affiliation: University of California Los Angeles, Department of Electrical Engineering, Los Angeles, CA 90095, USA.
authors.forename: Asael
authors.initials: A
authors.lastname: Papour
authors.forename: Zach
authors.initials: Z
authors.lastname: Taylor
authors.forename: Adria
authors.initials: A
authors.lastname: Sherman
authors.forename: Desiree
authors.initials: D
authors.lastname: Sanchez
authors.forename: Gregory
authors.initials: G
authors.lastname: Lucey
authors.forename: Linda
authors.initials: L
authors.lastname: Liau
authors.forename: Oscar
authors.initials: O
authors.lastname: Stafsudd
authors.forename: William
authors.initials: W
authors.lastname: Yong
authors.forename: Warren
authors.initials: W
authors.lastname: Grundfest
mesh_terms: D000465:Algorithms; D001921:Brain / Q000473:pathology*; D001932:Brain Neoplasms / Q000175:diagnosis / Q000473:pathology*; D002138:Calibration; D004867:Equipment Design; D005910:Glioma / Q000175:diagnosis / Q000473:pathology*; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008027:Light; D008856:Microscopy, Fluorescence / Q000379:methods*; D018715:Microscopy, Video / Q000295:instrumentation* / Q000379:methods*; D013048:Specimen Handling; D041623:Tomography, Optical Coherence