title: In vitro tuberization of Chlorophytum Borivilianum Sant & Fern (Safed musli) as influenced by sucrose, CCC and culture systems.
abstract: This study focuses on the establishment of in vitro tuberization of Chlorophytum borivilianum using solid and liquid culture systems. A high in vitro tuberization rate on solid and stationary liquid Murashige and Skoog media was observed in the presence of 60 g l⁻¹ sucrose with 950, 1,265 and 1,580 µM 2-chloroethyl-trimethylammonium chloride (CCC). Application of a higher sucrose concentration of 90 g l⁻¹ showed a negative interaction with CCC on in vitro tuber number and days to in vitro tuber induction. For economic feasibility, 950 µM CCC with 60 g l⁻¹ sucrose was chosen as the best combination for in vitro tuberization in both solid and stationary liquid media. For optimization of in vitro tuber production,a comparison between solid, stationary liquid and shake liquid culture was carried out. Liquid culture with shaking at 80 r.p.m. resulted in a >2.5-fold increase in in vitro tuber production compared with solid culture.
authors.affiliation: Department of Agriculture Technology, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Farshad Ashraf
authors.forename: Maheran
authors.initials: M
authors.lastname: Abd Aziz
authors.forename: Mihdzar
authors.initials: M
authors.lastname: Abdul Kadir
authors.forename: Johnson
authors.initials: J
authors.lastname: Stanslas
authors.forename: Elmira
authors.initials: E
authors.lastname: Farokhian
mesh_terms: D002716:Chlormequat / Q000494:pharmacology*; D046508:Culture Techniques / Q000379:methods*; D019668:Liliaceae / Q000187:drug effects* / Q000254:growth & development; D010937:Plant Growth Regulators / Q000494:pharmacology*; D018520:Plant Shoots / Q000187:drug effects / Q000254:growth & development; D035281:Plant Tubers / Q000187:drug effects* / Q000254:growth & development; D013395:Sucrose / Q000494:pharmacology*
chemical_list: D010937:Plant Growth Regulators; D013395:Sucrose; D002716:Chlormequat
keywords: Chlorophytum borivilianum; Hyperhydricity; In vitro tuberization; Liquid culture; Solid culture