title: A novel method for evaluating natural and vaccine induced serological responses to Bordetella pertussis antigens.
abstract: We studied the time course of serum IgG antibodies against 3 different pertussis vaccine antigens: PT (pertussis toxin), FHA (filamentous hemagglutinin), Prn (pertactin) in sera from individuals vaccinated with four different pertussis vaccines at 4 years of age: (N=44, 44, 23 and 23, respectively,) and compared the responses to/after natural infection with Bordetella pertussis (N=44, age 1-8 years). These longitudinal data were analyzed with a novel method, using a mathematical model to describe the observed responses, and their variation among subjects. This allowed us to estimate biologically meaningful characteristics of the serum antibody response, like peak level and decay rate, and to compare these among natural infections and vaccine responses. Compared to natural infection, responses to PT after vaccination with the tested vaccines are smaller in magnitude and tend to decay slightly faster. When present in vaccines, FHA and Prn tend to produce high peak levels, higher than those in naturally infected patients, but these decay faster. As expected, the Dutch whole cell vaccine produced lower antibody responses than the acellular vaccines. This model allows a better comparison of the kinetics of vaccine induced antibody responses and after natural infection over a long follow up period.
authors.affiliation: Center for Infectious Disease Control, National Institute of Public Health and the Environment, Antonie van Leeuwenhoeklaan 9, 3721 MA Bilthoven, The Netherlands.
authors.forename: G A M
authors.initials: GA
authors.lastname: Berbers
authors.forename: M S E
authors.initials: MS
authors.lastname: van de Wetering
authors.forename: P G M
authors.initials: PG
authors.lastname: van Gageldonk
authors.forename: J F P
authors.initials: JF
authors.lastname: Schellekens
authors.forename: F G A
authors.initials: FG
authors.lastname: Versteegh
authors.forename: P F M
authors.initials: PF
authors.lastname: Teunis
mesh_terms: D000907:Antibodies, Bacterial / Q000097:blood*; D000917:Antibody Formation / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D006388:Hemagglutinins / Q000276:immunology; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007223:Infant; D008137:Longitudinal Studies; D017711:Nonlinear Dynamics; D037342:Pertussis Toxin / Q000276:immunology; D010567:Pertussis Vaccine / Q000276:immunology*; D016032:Randomized Controlled Trials as Topic; D022282:Vaccines, Acellular / Q000276:immunology; D010566:Virulence Factors, Bordetella / Q000276:immunology; D014917:Whooping Cough / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D001425:Bacterial Outer Membrane Proteins; D006388:Hemagglutinins; D007074:Immunoglobulin G; D010567:Pertussis Vaccine; D022282:Vaccines, Acellular; D010566:Virulence Factors, Bordetella; C068929:pertactin; D037342:Pertussis Toxin
keywords: Acellular; Infection; Mixed model; Pertussis; Serology; Vaccine; Whole cell