title: Isolation of Chlamydomonas flagella.
abstract: A simple, scalable, and fast procedure for the isolation of Chlamydomonas flagella is described. Chlamydomonas can be synchronously deflagellated by treatment with chemicals, pH shock, or mechanical shear. The Basic Protocol describes the procedure for flagellar isolation using dibucaine to induce flagellar abscission; we also describe the pH shock method as an Alternate Protocol when flagellar regeneration is desirable. Sub-fractionation of the isolated flagella into axonemes and the membrane + matrix fraction is described in a Support Protocol.
authors.affiliation: Department of Cell and Developmental Biology, University of Massachusetts Medical School, Worcester, Massachusetts.
authors.forename: Branch
authors.initials: B
authors.lastname: Craige
authors.affiliation: Department of Cell and Developmental Biology, University of Massachusetts Medical School, Worcester, Massachusetts.
authors.forename: Jason M
authors.initials: JM
authors.lastname: Brown
authors.affiliation: Department of Cell and Developmental Biology, University of Massachusetts Medical School, Worcester, Massachusetts.
authors.forename: George B
authors.initials: GB
authors.lastname: Witman
mesh_terms: D054468:Axoneme / Q000648:ultrastructure; D002458:Cell Fractionation / Q000379:methods*; D048708:Cell Growth Processes; D002462:Cell Membrane / Q000648:ultrastructure; D002696:Chlamydomonas / Q000648:ultrastructure*; D003992:Dibucaine; D005407:Flagella / Q000648:ultrastructure*; D006863:Hydrogen-Ion Concentration; D013314:Stress, Mechanical
chemical_list: D003992:Dibucaine