title: A peptide microarray fabricated on a non-fouling phosphatidylcholine-polymer-coated surface for a high-fidelity analysis of a cellular kinome.
abstract: Phosphatidylcholine-polymer-coated plastic slides were utilized for the fabrication of peptide microarrays for cellular kinome analysis. According to the non-fouling features of the surface, the signal-to-noise ratio of the detection of phosphorylated peptides improved by about 100-fold from that of a peptide microarray fabricated on a glass slide blocked by a commercial BSA-based reagent. When the phosphatidylcholine-polymer-coated peptide microarray was applied to the analysis of the kinome of HCC827 cells, hyperactivation of c-Src and EGFR were successfully detected.
authors.affiliation: Department of Applied Chemistry, Kyushu University, 744 Moto-oka Nishi-ku, Fukuoka 819-0395, Japan. mori.takeshi.880@m.kyushuu.ac.jp.
authors.forename: H
authors.initials: H
authors.lastname: Ikeda
authors.forename: J
authors.initials: J
authors.lastname: Kamimoto
authors.forename: T
authors.initials: T
authors.lastname: Yamamoto
authors.forename: A
authors.initials: A
authors.lastname: Hata
authors.forename: Y
authors.initials: Y
authors.lastname: Otsubo
authors.forename: T
authors.initials: T
authors.lastname: Niidome
authors.forename: M
authors.initials: M
authors.lastname: Fukushima
authors.forename: T
authors.initials: T
authors.lastname: Mori
authors.forename: Y
authors.initials: Y
authors.lastname: Katayama
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism*; D005456:Fluorescent Dyes; D005898:Glass / Q000737:chemistry*; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism; D010446:Peptide Fragments / Q000032:analysis* / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry*; D010750:Phosphoproteins / Q000032:analysis; D010766:Phosphorylation; D011108:Polymers / Q000737:chemistry*; D040081:Protein Array Analysis; D011494:Protein Kinases / Q000032:analysis*; D014407:Tumor Cells, Cultured
chemical_list: D005456:Fluorescent Dyes; D010446:Peptide Fragments; D010713:Phosphatidylcholines; D010750:Phosphoproteins; D011108:Polymers; D011494:Protein Kinases