title: Maternal admixture and population structure in Mexican-Mestizos based on mtDNA haplogroups.
abstract: The maternal ancestry (mtDNA) has important applications in different research fields, such as evolution, epidemiology, identification, and human population history. This is particularly interesting in Mestizos, which constitute the main population in Mexico (∼93%) resulting from post-Columbian admixture between Spaniards, Amerindians, and African slaves, principally. Consequently, we conducted minisequencing analysis (SNaPshot) of 11 mitochondrial single-nucleotide polymorphisms in 742 Mestizos of 10 populations from different regions in Mexico. The predominant maternal ancestry was Native American (92.9%), including Haplogroups A, B, C, and D (47, 23.7, 15.9, and 6.2%, respectively). Conversely, European and African ancestries were less frequent (5.3 and 1.9%, respectively). The main characteristics of the maternal lineages observed in Mexican-Mestizos comprised the following: 1) contrasting geographic gradient of Haplogroups A and C; 2) increase of European lineages toward the Northwest; 3) low or absent, but homogeneous, African ancestry throughout the Mexican territory; 4) maternal lineages in Mestizos roughly represent the genetic makeup of the surrounding Amerindian groups, particularly toward the Southeast, but not in the North and West; 5) continuity over time of the geographic distribution of Amerindian lineages in Mayas; and 6) low but significant maternal population structure (FST  = 2.8%; P = 0.0000). The average ancestry obtained from uniparental systems (mtDNA and Y-chromosome) in Mexican-Mestizos was correlated with previous ancestry estimates based on autosomal systems (genome-wide single-nucleotide polymorphisms and short tandem repeats). Finally, the comparison of paternal and maternal lineages provided additional information concerning the gender bias admixture, mating patterns, and population structure in Mestizos throughout the Mexican territory.
authors.affiliation: Instituto de Investigación en Genética Molecular, Centro Universitario de la Ciénega, Universidad de Guadalajara, Ocotlán, Jalisco, México.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Martínez-Cortés
authors.forename: Joel
authors.initials: J
authors.lastname: Salazar-Flores
authors.forename: Javier
authors.initials: J
authors.lastname: Haro-Guerrero
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Rubi-Castellanos
authors.forename: Jésus S
authors.initials: JS
authors.lastname: Velarde-Félix
authors.forename: José F
authors.initials: JF
authors.lastname: Muñoz-Valle
authors.forename: Mavil
authors.initials: M
authors.lastname: López-Casamichana
authors.forename: Eduardo
authors.initials: E
authors.lastname: Carrillo-Tapia
authors.forename: Luis M
authors.initials: LM
authors.lastname: Canseco-Avila
authors.forename: Claudio M
authors.initials: CM
authors.lastname: Bravi
authors.forename: Mauro
authors.initials: M
authors.lastname: López-Armenta
authors.forename: Héctor
authors.initials: H
authors.lastname: Rangel-Villalobos
mesh_terms: D000704:Analysis of Variance; D044383:Black People / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics*; D003710:Demography; D004587:Electrophoresis, Agar Gel; D019075:Electrophoresis, Capillary; D014644:Genetic Variation; D005828:Genetics, Population; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D007198:Indians, North American / Q000235:genetics*; D008800:Mexico; D060885:Multiplex Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D017422:Sequence Analysis, DNA; D044465:White People / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial
keywords: Mexican-Mestizos; Y-SNPs; maternal admixture; mtDNA ancestry; mtSNPs