title: Aberrant glycosylation of plasma proteins in severe preeclampsia promotes monocyte adhesion.
abstract: Glycosylation of plasma proteins increases during pregnancy. Our objectives were to investigate an anti-inflammatory role of these proteins in normal pregnancies and determine whether aberrant protein glycosylation promotes monocyte adhesion in preeclampsia. Plasma was prospectively collected from nonpregnant controls and nulliparous patients in all 3 trimesters. Patients were divided into cohorts based on the applicable postpartum diagnosis. U937 monocytes were preconditioned with enzymatically deglycosylated plasma, and monocyte adhesion to endothelial cell monolayers was quantified by spectrophotometry. Plasma from nonpregnant controls, first trimester normotensives, and first trimester patients with mild preeclampsia inhibited monocyte-endothelial cell adhesion (P < .05), but plasma from first trimester patients with severe preeclampsia and second and third trimester normotensives did not. Deglycosylating plasma proteins significantly increased adhesion in all the cohorts. These results support a role of plasma glycoprotein interaction in monocyte-endothelial cell adhesion and could suggest a novel therapeutic target for severe preeclampsia.
authors.affiliation: 1Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Madigan Army Medical Center, Tacoma, WA, USA.
authors.forename: Shannon K
authors.initials: SK
authors.lastname: Flood-Nichols
authors.forename: Avedis A
authors.initials: AA
authors.lastname: Kazanjian
authors.forename: Deborah
authors.initials: D
authors.lastname: Tinnemore
authors.forename: Philip R
authors.initials: PR
authors.lastname: Gafken
authors.forename: Yuko
authors.initials: Y
authors.lastname: Ogata
authors.forename: Peter G
authors.initials: PG
authors.lastname: Napolitano
authors.forename: Jonathan D
authors.initials: JD
authors.lastname: Stallings
authors.forename: Danielle L
authors.initials: DL
authors.lastname: Ippolito
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D001798:Blood Proteins / Q000235:genetics / Q000378:metabolism*; D002448:Cell Adhesion / Q000502:physiology; D015331:Cohort Studies; D005260:Female; D006031:Glycosylation; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008969:Molecular Sequence Data; D009000:Monocytes / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000097:blood* / Q000175:diagnosis* / Q000235:genetics; D011247:Pregnancy; D012720:Severity of Illness Index; D020298:U937 Cells; D055815:Young Adult
chemical_list: D001798:Blood Proteins
keywords: adhesion; glycosylation; monocyte; pregnancy; severe preeclampsia