title: HPTLC-densitometry determination of triterpenic acids in Origanum vulgare, Rosmarinus officinalis and Syzygium aromaticum.
abstract: Spices play an important role in the chemoprevention and they can be a rich source of biologically active compounds such as triterpenes in the human diet. A method based on high performance thin-layer chromatography combined with densitometry for determination of ursolic and oleanolic acids in some common spices was elaborated. The prechromatographic derivatization with 1% of iodine solution was used to enable simultaneous analysis of these triterpenes. The extracts were separated on HPTLC silica gel 60 F254 plates with use of mobile phase consisting of toluene-petroleum ether-ethyl acetate-acetonitrile 5: 5: 1: 0.3 (v/v/v/v). After drying, the plates were sprayed with 10% (v/v) ethanol solution of sulfuric acid (VI) and heated to 120 degrees C for 3 min. Quantification was performed in fluorescence/reflectance mode at a wavelength of 400 nm using a computer-controlled densitometer Desaga CD 60.
authors.affiliation: Department of Pharmaceutical Botany, Medical University, Chodźki 1, 20-093 Lublin, Poland.
authors.forename: Renata
authors.initials: R
authors.lastname: Nowak
authors.forename: Magdalena
authors.initials: M
authors.lastname: Wójciak-Kosior
authors.forename: Ireneusz
authors.initials: I
authors.lastname: Sowa
authors.forename: Anna
authors.initials: A
authors.lastname: Sokołowska-Krzaczek
authors.forename: Wioleta
authors.initials: W
authors.lastname: Pietrzak
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Szczodra
authors.forename: Ryszard
authors.initials: R
authors.lastname: Kocjan
mesh_terms: D002855:Chromatography, Thin Layer / Q000379:methods*; D003720:Densitometry / Q000379:methods*; D009828:Oleanolic Acid / Q000032:analysis*; D031341:Origanum / Q000737:chemistry*; D027542:Rosmarinus / Q000737:chemistry*; D027842:Syzygium / Q000737:chemistry*; D014315:Triterpenes / Q000032:analysis*; D000097245:Ursolic Acid
chemical_list: D009828:Oleanolic Acid; D014315:Triterpenes; D000097245:Ursolic Acid