title: Multiple silk coatings on biphasic calcium phosphate scaffolds: effect on physical and mechanical properties and in vitro osteogenic response of human mesenchymal stem cells.
abstract: Ceramic scaffolds such as biphasic calcium phosphate (BCP) have been widely studied and used for bone regeneration, but their brittleness and low mechanical strength are major drawbacks. We report the first systematic study on the effect of silk coating in improving the mechanical and biological properties of BCP scaffolds, including (1) optimization of the silk coating process by investigating multiple coatings, and (2) in vitro evaluation of the osteogenic response of human mesenchymal stem cells (hMSCs) on the coated scaffolds. Our results show that multiple silk coatings on BCP ceramic scaffolds can achieve a significant coating effect to approach the mechanical properties of native bone tissue and positively influence osteogenesis by hMSCs over an extended period. The silk coating method developed in this study represents a simple yet effective means of reinforcement that can be applied to other types of ceramic scaffolds with similar microstructure to improve osteogenic outcomes.
authors.affiliation: Department of Biomedical Engineering, Tufts University, Medford, Massachusetts 02155, United States.
authors.forename: Jiao Jiao
authors.initials: JJ
authors.lastname: Li
authors.forename: Eun Seok
authors.initials: ES
authors.lastname: Gil
authors.forename: Rebecca S
authors.initials: RS
authors.lastname: Hayden
authors.forename: Chunmei
authors.initials: C
authors.lastname: Li
authors.forename: Seyed-Iman
authors.initials: SI
authors.lastname: Roohani-Esfahani
authors.forename: David L
authors.initials: DL
authors.lastname: Kaplan
authors.forename: Hala
authors.initials: H
authors.lastname: Zreiqat
mesh_terms: D000328:Adult; D000469:Alkaline Phosphatase / Q000235:genetics; D001861:Bone Regeneration; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation; D002478:Cells, Cultured; D002516:Ceramics / Q000737:chemistry; D024042:Collagen Type I / Q000235:genetics; D015870:Gene Expression; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) / Q000235:genetics; D006801:Humans; D006882:Hydroxyapatites / Q000737:chemistry / Q000494:pharmacology*; D058574:Integrin-Binding Sialoprotein / Q000235:genetics; D008297:Male; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000201:enzymology / Q000378:metabolism*; D010006:Osteoblasts / Q000166:cytology / Q000502:physiology; D010012:Osteogenesis / Q000187:drug effects* / Q000502:physiology; D016062:Porosity; D047011:Silk / Q000737:chemistry / Q000494:pharmacology*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D024042:Collagen Type I; D006882:Hydroxyapatites; D058574:Integrin-Binding Sialoprotein; D047011:Silk; C074950:hydroxyapatite-beta tricalcium phosphate; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+); D000469:Alkaline Phosphatase