title: HPLC with quadrupole TOF-MS and chemometrics analysis for the characterization of Folium Turpiniae from different regions.
abstract: Folium Turpiniae has been used as a traditional Chinese medicine for the treatment of abscesses, fevers, gastric ulcers, and inflammations. This paper describes a strategy of combining HPLC with photodiode array detection and quadrupole TOF-MS, as well as phytochemical and chemometrics analysis for the characterization, isolation, and simultaneous quantification of the chemical constituents of Folium Turpiniae. 19 constituents were identified, namely, 11 flavonoids, seven gallic acid derivates, and quinic acid. Among them, 15 compounds were identified in this herbal medicine for the first time; compound 10 appears to be novel and was isolated and confirmed as ellagic acid-3-O-α-L-rhamnopyranoside by NMR spectroscopy and MS. In addition, nine marker compounds, namely gallic acid (2), ellagic acid-3-O-α-L-rhamnopyranoside (10), apigenin-7-O-(2''-rhamnosyl)gentiobioside (11), ellagic acid (12), luteolin-7-O-β-D-neohesperidoside (13), ligustroflavone (14), 4'-O-methylellagic acid-3-O-α-L-rhamnopyranoside (16), rhoifolin (17), and neobudofficide (18), were quantified simultaneously in ten batches of Folium Turpiniae collected from different regions. Moreover, hierarchical clustering analysis and principal component analysis indicated that both samples from Hubei (S1) and Guangxi (S3) provinces showed apparent differences from the others. Samples from Jiangxi province (S2, S4, and S7-10) possessed similar properties and therefore belong to the same group.
authors.affiliation: Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: Yanyang
authors.initials: Y
authors.lastname: Zhao
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Liu
authors.forename: Feng
authors.initials: F
authors.lastname: Feng
authors.forename: Ning
authors.initials: N
authors.lastname: Xie
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis*; D005707:Gallic Acid / Q000032:analysis*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011801:Quinic Acid / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D005419:Flavonoids; D010936:Plant Extracts; D011801:Quinic Acid; D005707:Gallic Acid
keywords: Chemical constituents; Chemometrics analysis; Folium Turpiniae; Quadrupole time-of-flight mass spectrometry; Simultaneous quantification