title: Identification and roles of proteins for seed development in mungbean (Vigna radiata L.) seed proteomes.
abstract: Proteomic analysis of developing mungbean (Vigna radiata L.) seeds has not yet been investigated in detail. Fifty-seven proteins were separated by 2-DE, identified by nanoelectrospray mass spectrometry from the present protein databases, and categorized according to their functions. Many of the identified enzymes were involved in central carbon metabolism; thus, a pathway illustrating starch synthesis/breakdown, sugar conversion for glycolysis, and tricarboxylic acid (TCA) cycle was proposed. Quantitative comparison of the protein expression revealed that during developmental process (11-21 days after flowering, DAF), proteins involved in glycolysis, TCA cycle, and alcoholic fermentation showed a trend to be down-regulated, whereas storage proteins were generally up-regulated. The downward tendency of central carbon metabolic proteins suggests a reduction in ATP and oxygen consumption associated with accumulation of storage compounds. UDP-glucose-1-pyrophosphorylase, an upstream enzyme in the starch ADP-Glc pathway, was found as a stably expressed protein throughout the growth stage, demonstrating its importance in mungbean starch biosynthesis. The temporal expression of metabolic enzymes suggests the coordination of an acclimation mechanism and cellular processes associated with accumulation of storage compounds in seed development.
authors.affiliation: Department of Food Science, Biotechnology Division, College of Life Sciences, National Taiwan Ocean University , 2 Pei-Ning Road, Keelung 20224, Taiwan, Republic of China (ROC).
authors.forename: Bartosz
authors.initials: B
authors.lastname: Kazłowski
authors.forename: Mao-Rong
authors.initials: MR
authors.lastname: Chen
authors.forename: Pei-Min
authors.initials: PM
authors.lastname: Chao
authors.forename: Chien-Chen
authors.initials: CC
authors.lastname: Lai
authors.forename: Yuan-Tih
authors.initials: YT
authors.lastname: Ko
mesh_terms: D002952:Citric Acid Cycle; D007887:Fabaceae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D012639:Seeds / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D020543:Proteome