title: Promoter methylation of GATA4, WIF1, NTRK1 and other selected tumour suppressor genes in ovarian cancer.
abstract: Ovarian cancer is the leading cause of death from gynaecologic tumours, but the molecular and especially epigenetic events underlying the transformation are poorly understood. Various methylation changes have been identified and show promise as potential cancer biomarkers. The aim of this study was to investigate promoter methylation of selected tumour suppressor genes in ovarian cancer by comparison with normal ovarian tissue. To search for epigenetic events we used methylation-specific multiplex ligation-dependent probe amplification to compare the methylation status of 44 tissue samples of ovarian cancer with 30 control samples. Using a 20% cut-off for methylation, we observed significantly higher methylation in genes NTKR1, GATA4 and WIF1 in the ovarian cancer group compared with the control group. These findings could potentially be used in screening of ovarian cancer, and may have implications for future chemotherapy based on epigenetic changes.
authors.affiliation: Institute for Clinical Biochemistry and Diagnostics, Charles University in Prague - Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Czech Republic. ChmelarovaMarcela@seznam.cz
authors.forename: M
authors.initials: M
authors.lastname: Chmelařová
authors.forename: E
authors.initials: E
authors.lastname: Dvořáková
authors.forename: J
authors.initials: J
authors.lastname: Špaček
authors.forename: J
authors.initials: J
authors.lastname: Laco
authors.forename: M
authors.initials: M
authors.lastname: Mžik
authors.forename: V
authors.initials: V
authors.lastname: Palička
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics* / Q000378:metabolism; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016022:Case-Control Studies; D019175:DNA Methylation / Q000235:genetics*; D005260:Female; D050991:GATA4 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000235:genetics / Q000473:pathology; D011401:Promoter Regions, Genetic; D020917:Receptor, trkA / Q000235:genetics* / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics* / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000235:genetics* / Q000378:metabolism; D055815:Young Adult
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D050991:GATA4 Transcription Factor; C484170:GATA4 protein, human; D012097:Repressor Proteins; D025521:Tumor Suppressor Proteins; C485569:WIF1 protein, human; D020917:Receptor, trkA