title: Ogataea kolombanensis sp. nov., Ogataea histrianica sp. nov. and Ogataea deakii sp. nov., three novel yeast species from plant sources.
abstract: Nine methanol-assimilating yeast strains isolated from olive oil sediments in Slovenia, extra virgin olive oil from Italy and rotten wood collected in Hungary were found to form three genetically separated groups, distinct from the currently recognized yeast species. Sequence analysis from genes of the small subunit (SSU) rRNA, internal transcribed spacer region/5.8S rRNA, large subunit (LSU) rRNA D1/D2 domains and translational elongation factor-1α (EF-1α) revealed that the three closely related groups represent three different undescribed yeast species. Sequence analysis of the LSU rRNA gene D1/D2 domains placed the novel species in the Ogataea clade. The three novel species are designated as Ogataea kolombanensis sp. nov. (type strain: ZIM 2322(T) = CBS 12778(T) = NRRL Y-63657(T)), Ogataea histrianica sp. nov. (type strain: ZIM 2463(T) = CBS 12779(T) = NRRL Y-63658(T)) and Ogataea deakii sp. nov. (type strain: NCAIM Y.01896(T) = CBS 12735(T) = NRRL Y-63656(T)).
authors.affiliation: Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia.
authors.forename: Neža
authors.initials: N
authors.lastname: Čadež
authors.affiliation: National Collection of Agricultural and Industrial Microorganisms, Faculty of Food Science, Corvinus University of Budapest, Somlói út 14-16. H-1118 Budapest, Hungary.
authors.forename: Dénes
authors.initials: D
authors.lastname: Dlauchy
authors.affiliation: Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia.
authors.forename: Peter
authors.initials: P
authors.lastname: Raspor
authors.affiliation: National Collection of Agricultural and Industrial Microorganisms, Faculty of Food Science, Corvinus University of Budapest, Somlói út 14-16. H-1118 Budapest, Hungary.
authors.forename: Gábor
authors.initials: G
authors.lastname: Péter
mesh_terms: D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D005516:Food Microbiology; D006814:Hungary; D007558:Italy; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D000069463:Olive Oil; D020648:Peptide Elongation Factor 1 / Q000235:genetics; D010802:Phylogeny; D010938:Plant Oils; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics; D054683:Ribosome Subunits, Large, Eukaryotic / Q000382:microbiology; D004718:Saccharomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D017524:Slovenia; D014934:Wood / Q000382:microbiology
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D000069463:Olive Oil; D020648:Peptide Elongation Factor 1; D010938:Plant Oils; D012340:RNA, Ribosomal, 5.8S