title: Optimized simultaneous saccharification and co-fermentation of rice straw for ethanol production by Saccharomyces cerevisiae and Scheffersomyces stipitis co-culture using design of experiments.
abstract: Herein an ethanol production process from rice straw was optimized. Simultaneous saccharification and co-fermentation (SSCF) using Saccharomyces cerevisiae and Scheffersomyces stipitis co-culture was carried out to enhance ethanol production. The optimal saccharification solid loading was 5%. Key fermentation parameters for co-culture including cell ratio, agitation rate and temperature was rationally optimized using design of experiment (DoE). Optimized co-culture conditions for maximum ethanol production efficiency were at S. cerevisiae:S. stipitis cell ratio of 0.31, agitation rate of 116 rpm and temperature of 33.1°C. The optimized SSCF process reached ethanol titer of 15.2g/L and ethanol yield of 99% of theoretical yield, consistent with the DoE model prediction. Moreover, SSCF process under high biomass concentration resulted in high ethanol concentration of 28.6g/L. This work suggests the efficiency and scalability of the developed SSCF process which could provide an important basis for the economic feasibility of ethanol production from lignocelluloses.
authors.affiliation: The Joint Graduate School of Energy and Environment, King Mongkut's University of Technology Thonburi, 126 Pracha Uthit Rd., Tungkru, Bangkok 10140, Thailand.
authors.forename: Nopparat
authors.initials: N
authors.lastname: Suriyachai
authors.forename: Khatiya
authors.initials: K
authors.lastname: Weerasaia
authors.forename: Navadol
authors.initials: N
authors.lastname: Laosiripojana
authors.forename: Verawat
authors.initials: V
authors.lastname: Champreda
authors.forename: Pornkamol
authors.initials: P
authors.lastname: Unrean
mesh_terms: D018533:Biomass; D018920:Coculture Techniques; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D012275:Oryza / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
chemical_list: D000431:Ethanol
keywords: Co-culture; Design of experiment; Ethanol; Simultaneous saccharification and co-fermentation (SSCF) optimization