title: Oxidative stress and free-radical oxidation in bcg granulomatosis development.
abstract: BACKGROUND
  Little is known about the role of free-radical and oxidative stress signaling in granuloma maturation and resolution. We aimed to study the activity of free-radical oxidation processes in the dynamics of BCG-induced generalized granulomatosis in mice.
  
  
  METHODS
  Chronic granulomatous inflammation was induced in male BALB/c mice by intravenously injecting the BCG vaccine, and the production of oxidative stress (activity of free-radical oxidation processes) and histological changes in the lungs, liver, and peritoneal exudate were measured 3, 30, 60, and 90 days after infection.
  
  
  RESULTS
  The tuberculous granuloma numerical density and diameter continuously increased from day 30 to day 90, and the macrophage content within the granulomas progressively diminished with a concomitant elevation in the number of epithelioid cells. The activity of the free-radical oxidation processes in the liver (i.e., the intensity of the homogenate chemiluminescence) reached a maximum at postinfection day 60 and subsequently began to decrease. The peak generation of reactive oxygen species by phagocytes in the peritoneal exudate (measured using flow cytometry) was also shifted in time and fell on day 30.
  
  
  CONCLUSIONS
  The rise in the steady-state concentration of H2O2 in the liver of mice with BCG-induced granulomatosis is not related to local H2O2 production by phagocytes, and a decrease in the severity of generalized inflammation precedes the resolution of local inflammation.
authors.affiliation: lemen@soramn.ru
authors.forename: Elena
authors.initials: E
authors.lastname: Menshchikova
authors.forename: Nikolay
authors.initials: N
authors.lastname: Zenkov
authors.forename: Victor
authors.initials: V
authors.lastname: Tkachev
authors.forename: Oksana
authors.initials: O
authors.lastname: Potapova
authors.forename: Liliya
authors.initials: L
authors.lastname: Cherdantseva
authors.forename: Vyacheslav
authors.initials: V
authors.lastname: Shkurupiy
mesh_terms: D000818:Animals; D001500:BCG Vaccine / Q000009:adverse effects*; D001835:Body Weight; D002452:Cell Count; D005453:Fluorescence; D005609:Free Radicals / Q000378:metabolism*; D006099:Granuloma / Q000209:etiology* / Q000473:pathology*; D006861:Hydrogen Peroxide / Q000378:metabolism; D008099:Liver / Q000473:pathology; D008163:Luminescent Measurements; D008165:Luminol / Q000378:metabolism; D008168:Lung / Q000473:pathology; D008264:Macrophages / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009504:Neutrophils / Q000473:pathology; D009929:Organ Size; D010084:Oxidation-Reduction; D018384:Oxidative Stress
chemical_list: D001500:BCG Vaccine; D005609:Free Radicals; D008165:Luminol; D006861:Hydrogen Peroxide