title: Direct L-lysine production from cellobiose by Corynebacterium glutamicum displaying beta-glucosidase on its cell surface.
abstract: We constructed beta-glucosidase (BGL)-displaying Corynebacterium glutamicum, and direct L-lysine fermentation from cellobiose was demonstrated. After screening active BGLs, Sde1394, which is a BGL from Saccharophagus degradans, was successfully displayed on the C. glutamicum cell surface using porin as an anchor protein, and cellobiose was directly assimilated as a carbon source. The optical density at 600 nm of BGL-displaying C. glutamicum grown on cellobiose as a carbon source reached 23.5 after 48 h of cultivation, which was almost the same as that of glucose after 24 h of cultivation. Finally, Sde1394-displaying C. glutamicum produced 1.08 g/l of L-lysine from 20 g/l of cellobiose after 4 days of cultivation, which was about threefold higher than the amount of produced L-lysine using BGL-secretory C. glutamicum strains (0.38 g/l after 5 days of cultivation). This is the first report on amino acid production using cellobiose as a carbon source by BGL-expressing C. glutamicum.
authors.affiliation: Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai-cho, Nada, Kobe 657-8501, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Adachi
authors.forename: Chihiro
authors.initials: C
authors.lastname: Takahashi
authors.forename: Naoko
authors.initials: N
authors.lastname: Ono-Murota
authors.forename: Rie
authors.initials: R
authors.lastname: Yamaguchi
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Tanaka
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
mesh_terms: D034241:Alteromonadaceae / Q000201:enzymology / Q000235:genetics; D061505:Cell Surface Display Techniques; D002475:Cellobiose / Q000378:metabolism*; D048230:Corynebacterium glutamicum / Q000235:genetics / Q000378:metabolism*; D008239:Lysine / Q000096:biosynthesis*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D001617:beta-Glucosidase / Q000235:genetics / Q000378:metabolism*
chemical_list: D011994:Recombinant Proteins; D002475:Cellobiose; D001617:beta-Glucosidase; D008239:Lysine