title: Downregulation of super oxide dismutase level in protein might be due to sulfur mustard induced toxicity in lung.
abstract: Sulfur mustard (SM) has been identified as an important chemical weapon. During the Iran-Iraq war of 1980-88, the extensive usage of SM against Iranian civilians and military forces was proven. This agent has been shown to cause severe damage mainly in the skin, eyes, lungs, and respiratory tract in Iranian veterans. The most common disease is bronchiolitis obliterans (BO)). SM increases the endogenous production of reactive oxygen species (ROS). Superoxide dismutases (SODs) are known as protective antioxidants against the harmful effects of ROS. Twenty exposed SM individuals (43.2±6.4 years), and 10 normal controls (41.3±2.5 years) were enrolled in this study. Evaluation of SODs was performed by semiquantitative RT-PCR and immunohistochemistry. Our results demonstrated that CuZnSOD and MnSOD mRNA were up-regulated 2.79±1.09 and 2.49±1.11 folds, respectively in SM-injured patients in comparison with control levels. In contrast, Immunohistochemistry results showed downregulation of CuZnSOD protein expression in SM injured patients. Our results revealed that SODs may play an important role in cellular protection against oxidative stress due to mustard gas toxicity in airway wall of SM exposed patients.
authors.affiliation: Genomics Division, Systems Biology Institute. Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran and Biochemistry Department, Sciences and Technology Branch, Azad University, Tehran, Iran. r.nourani@yahoo.com.
authors.forename: Leila
authors.initials: L
authors.lastname: Mirbagheri
authors.forename: Mehryar
authors.initials: M
authors.lastname: Habibi Roudkenar
authors.forename: Abbas Ali
authors.initials: AA
authors.lastname: Imani Fooladi
authors.forename: Mostafa
authors.initials: M
authors.lastname: Ghanei
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Nourani
mesh_terms: D000328:Adult; D001999:Bronchoscopy; D002619:Chemical Warfare Agents / Q000009:adverse effects*; D015536:Down-Regulation; D006801:Humans; D007150:Immunohistochemistry; D008168:Lung / Q000187:drug effects* / Q000201:enzymology*; D008297:Male; D008875:Middle Aged; D009151:Mustard Gas / Q000009:adverse effects*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013482:Superoxide Dismutase / Q000032:analysis / Q000378:metabolism*
chemical_list: D002619:Chemical Warfare Agents; D013482:Superoxide Dismutase; D009151:Mustard Gas