title: Recruitment of exogenous mesenchymal stem cells in mandibular distraction osteogenesis by the stromal cell-derived factor-1/chemokine receptor-4 pathway in rats.
abstract: Distraction osteogenesis is widely used in orthopaedic and craniofacial surgery. However, its exact mechanism is still poorly understood. The purpose of this study was to find out whether there is systemic recruitment of mesenchymal stem cells (MSC) to the neocallus in the distraction gap by the stromal cell-derived factor-1 (SDF-1)/CXC chemokine receptor 4 (CXCR4) axis during osteogenesis. We examined the migration of MSC towards a gradient of SDF-1 in vitro. We also transplanted MSC labelled with green fluorescent protein (GFP) intravenously, with or without treatment with CXCR4-blocking antibody, into rats that had had unilateral mandibular distraction osteogenesis, and investigated the distribution of cells labelled with GFP in the soft callus after 24 h. We found that SDF-1 facilitated the migration potency of MSC both in vitro and in vivo, and this migration could be inhibited by AMD3100, an antagonist of CXCR4, and promoted by local infusion of exogenous SDF-1 into the distraction gap. This study provides a new insight into the molecular basis of how new bone is regenerated during distraction osteogenesis.
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University, Xi'an, China; Department of Oral and Maxillofacial Surgery, The Military General Hospital of Lanzhou Command, Lanzhou, China. Electronic address: j.cao@live.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Cao
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Zhao-jie
authors.initials: ZJ
authors.lastname: Du
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.forename: Ya-bo
authors.initials: YB
authors.lastname: Zhang
authors.forename: Jian-fu
authors.initials: JF
authors.lastname: Sui
authors.forename: Yan-pu
authors.initials: YP
authors.lastname: Liu
authors.forename: De-lin
authors.initials: DL
authors.lastname: Lei
mesh_terms: D000818:Animals; D001596:Benzylamines; D001861:Bone Regeneration / Q000187:drug effects; D002146:Bony Callus / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D054377:Chemokine CXCL12 / Q000494:pharmacology*; D002633:Chemotaxis / Q000187:drug effects* / Q000502:physiology; D000080027:Cyclams; D015194:Diffusion Chambers, Culture; D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins; D006571:Heterocyclic Compounds / Q000494:pharmacology; D008297:Male; D008334:Mandible / Q000601:surgery*; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000502:physiology; D010012:Osteogenesis / Q000187:drug effects; D019857:Osteogenesis, Distraction / Q000295:instrumentation / Q000379:methods; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D019718:Receptors, CXCR4 / Q000037:antagonists & inhibitors / Q000502:physiology*; D013997:Time Factors
chemical_list: D001596:Benzylamines; D054377:Chemokine CXCL12; C486264:Cxcr4 protein, rat; D000080027:Cyclams; D005456:Fluorescent Dyes; D006571:Heterocyclic Compounds; D019718:Receptors, CXCR4; D049452:Green Fluorescent Proteins; C088327:plerixafor
keywords: CXC chemokine receptor 4; Distraction osteogenesis; Homing; Mesenchymal stem cells; Stromal cell-derived factor-1