title: [Detection of autophagy based on conversions of MAP1LC3 and p62/SQSTM1].
abstract: Autophagy is a cellular process fundamental for the survival of nutrient deficiency periods and for organelle turnover. Recently much attention has been focused on autophagy as its impairment has been found in many human diseases. Unfortunately, our apparatus for study of the autophagy process is still unsatisfactory and not very well known. In this paper we would like to shed light on and discuss autophagy methods. We present the methods (fluorescence and Western blotting) based on conversions of MAP1LC3 and p62/SQSTM1 proteins, which are the most common markers of the autophagy process.
authors.affiliation: Laboratorium Immunobiologii Molekularnej Nowotworów, Instytut Immunologii i Terapii Doświadczalnej im Ludwika Hirszfelda PAN we Wrocławiu, Wrocław.
authors.forename: Edyta
authors.initials: E
authors.lastname: Wysokińska
authors.forename: Wojciech
authors.initials: W
authors.lastname: Kałas
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D001343:Autophagy / Q000502:physiology*; D015153:Blotting, Western; D006801:Humans; D017874:Immediate-Early Proteins / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D000071456:Sequestosome-1 Protein
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D017874:Immediate-Early Proteins; C477012:MAP1LC3A protein, human; D008869:Microtubule-Associated Proteins; C099718:SQSTM1 protein, human; D000071456:Sequestosome-1 Protein