title: Antibody-secreting cells with a phenotype of Ki-67low, CD138high, CD31high, and CD38high secrete nonspecific IgM during primary hepatitis A virus infection.
abstract: Although studies investigating the nature of Ab-secreting cells (ASCs) during acute infection with influenza or dengue virus found that the ASC response was dominated by virus-specific IgG secretion, the Ag specificity and phenotype of ASCs during primary acute viral infection were not identified. To this end, we investigated the nature of ASCs in direct ex vivo assays from patients with acute hepatitis A caused by primary infection with hepatitis A virus (HAV). We found that the frequency of CD27(high)CD38(high) ASCs was markedly increased in the peripheral blood during the acute phase of HAV infection. Moreover, substantial numbers of ASCs were non-HAV-specific and dominantly secreted IgM. We detected HAV-specific ASCs by staining with fluorochrome-tagged HAV-VP1 protein. As compared with HAV-specific ASCs, non-HAV-specific ASCs were Ki-67(low)CD138(high)CD31(high)CD38(high), demonstrating that non-HAV-specific ASCs had a bone marrow plasma cell-like phenotype whereas HAV-specific ASCs had a phenotype typical of circulating plasmablasts. These data suggest that non-HAV-specific ASCs might be mobilized plasma cells from the bone marrow or the spleen, whereas HAV-specific ASCs were newly generated plasmablasts. In this study, we provide evidence that pre-existing plasma cells are released into the circulation and contribute to Ag-nonspecific secretion of IgM during primary HAV infection.
authors.affiliation: Laboratory of Immunology and Infectious Diseases, Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Republic of Korea.
authors.forename: Seokchan
authors.initials: S
authors.lastname: Hong
authors.forename: Hyun Woong
authors.initials: HW
authors.lastname: Lee
authors.forename: Dong-Yeop
authors.initials: DY
authors.lastname: Chang
authors.forename: Sooseong
authors.initials: S
authors.lastname: You
authors.forename: Jihye
authors.initials: J
authors.lastname: Kim
authors.forename: Jun Yong
authors.initials: JY
authors.lastname: Park
authors.forename: Sang Hoon
authors.initials: SH
authors.lastname: Ahn
authors.forename: Dongeun
authors.initials: D
authors.lastname: Yong
authors.forename: Kwang-Hyub
authors.initials: KH
authors.lastname: Han
authors.forename: Ook Joon
authors.initials: OJ
authors.lastname: Yoo
authors.forename: Eui-Cheol
authors.initials: EC
authors.lastname: Shin
mesh_terms: D051997:ADP-ribosyl Cyclase 1 / Q000096:biosynthesis*; D000208:Acute Disease; D000328:Adult; D000918:Antibody Specificity; D000921:Antibody-Producing Cells / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D006506:Hepatitis A / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D007075:Immunoglobulin M / Q000096:biosynthesis*; D016130:Immunophenotyping; D019394:Ki-67 Antigen / Q000096:biosynthesis / Q000378:metabolism*; D007958:Leukocyte Count; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000096:biosynthesis*; D053668:Syndecan-1 / Q000096:biosynthesis*; D055815:Young Adult
chemical_list: D007075:Immunoglobulin M; D019394:Ki-67 Antigen; D008562:Membrane Glycoproteins; D019408:Platelet Endothelial Cell Adhesion Molecule-1; C508241:SDC1 protein, human; D053668:Syndecan-1; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1