title: Determination of water-soluble vitamins in multivitamin dietary supplements and in artichokes by micellar electrokinetic chromatography.
abstract: Several procedures of extraction with solvents for the simultaneous determination of vitamin C and some vitamins belonging to the B group (thiamine, riboflavine, nicotinic acid and nicotinamide) in multivitamin preparations and in artichokes (Cynara cardunculus subsp. scolymus [L.] Hegi) were developed. Different experimental conditions were used, in terms of heat treatment, composition and pH of the extraction mixture, with particular attention to high-temperature steps; purification of the extracts with solid phase extraction and stabilisation through lyophilisation were discussed. Analyses of the extracts were conducted by capillary electrophoresis in micellar electrokinetic chromatography modality. Borate buffer at pH 8.2 was used, and sodium dodecyl sulphate was added to the background electrolyte as surfactant. A range of linearity was determined and calibration curves were plotted for all the analytes.
authors.affiliation: a Dipartimento di Scienze Farmaceutiche , Facoltà di Farmacia , via Bonanno 6, 56100 , Pisa , Italy.
authors.forename: Enrico
authors.initials: E
authors.lastname: Serni
authors.forename: Valeria
authors.initials: V
authors.lastname: Audino
authors.forename: Sara
authors.initials: S
authors.lastname: Del Carlo
authors.forename: Clementina
authors.initials: C
authors.lastname: Manera
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Saccomanni
authors.forename: Marco
authors.initials: M
authors.lastname: Macchia
mesh_terms: D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D031943:Cynara scolymus / Q000737:chemistry*; D019587:Dietary Supplements / Q000032:analysis*; D012995:Solubility; D014815:Vitamins / Q000032:analysis*; D014867:Water / Q000737:chemistry*
chemical_list: D014815:Vitamins; D014867:Water