title: The transmembrane domain of the T4SS coupling protein TrwB and its role in protein-protein interactions.
abstract: Bacteria use type IV secretion systems to transfer genetic material and proteins from donor to recipient cells, using proteins encoded by conjugative plasmids. Among those proteins the so-called Type IV Coupling Protein plays a central role in the process. One of the best studied members of this family is TrwB, the conjugative coupling protein of R388 plasmid. Previous studies indicated that the transmembrane domain of TrwB plays a role beyond the mere anchoring of the protein to the membrane. TrwB has also been shown to interact with other conjugative proteins, such as the VirB10-like protein of R388 TrwE. The goal of this study is to elucidate the role of the different domains of TrwB and TrwE in their biological function, and in both self- and TrwB-TrwE interactions. To this aim, a series of TrwB and TrwE deletion mutant proteins were constructed. Conjugation and interaction studies revealed that the transmembrane domain of TrwB, and particularly its second transmembrane helix, is needed for TrwB self-interaction and for R388 conjugative transfer and that there are contacts between TrwB and TrwE in the membrane. On the contrary, the lack of the TMD of TrwE does not completely abolish R388 conjugation although the interaction between TrwE-TrwB is lost. These results identify protein-protein interactions inside the membrane needed for T4SS function.
authors.affiliation: Unidad de Biofísica (CSIC, UPV/EHU), and Departamento de Bioquímica y Biología Molecular, Universidad del País Vasco, Apdo. 644, 48080 Bilbao, Spain.
authors.forename: Rosa L
authors.initials: RL
authors.lastname: Segura
authors.forename: Sandra
authors.initials: S
authors.lastname: Aguila-Arcos
authors.forename: Begoña
authors.initials: B
authors.lastname: Ugarte-Uribe
authors.forename: Ana J
authors.initials: AJ
authors.lastname: Vecino
authors.forename: Fernando
authors.initials: F
authors.lastname: de la Cruz
authors.forename: Félix M
authors.initials: FM
authors.lastname: Goñi
authors.forename: Itziar
authors.initials: I
authors.lastname: Alkorta
mesh_terms: D002462:Cell Membrane / Q000737:chemistry* / Q000378:metabolism; D003227:Conjugation, Genetic / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D009154:Mutation; D010957:Plasmids; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D017433:Protein Structure, Secondary; D021381:Protein Transport
chemical_list: D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C403125:trwB protein, E coli
keywords: 2-keto-3-deoxyoctonate; BTH; Bacterial conjugation; Bartonella tribocorum TrwE homolog; Conjugative plasmid; Coupling protein; DDM; DTBP; HRP; IPTG; IR; KDO; PRR; T4CP; T4SS; TMD; TrwE(Bt); Type IV secretion system; VirB10-like proteins; bacterial two-hybrid; dimethyl 3,3′-dithiobispropionimidate; horseradish peroxidase; infrared spectroscopy; n-dodecyl-β-d-maltoside; proline rich region; transmembrane domain; type IV coupling protein; type IV secretion system; β-d-thiogalactopyranoside