title: Rapid Identification of the Multiple Absorbed Bioactive Components and Metabolites in Rat Serum after Oral Administration of Wu-Jia Sheng-Hua Capsule by UPLC-ESI-MS.
abstract: To identify the compounds absorbed in rat serum after the oral administration of Wu-Jia Sheng-Hua (WJSH) capsule, a traditional Chinese medicine (TCM) compound prescription, an ultraperformance liquid chromatography coupled with electrospray ionization mass spectrometry (UPLC/ESI-MS) method, was established. The chromatographic separation of the absorbed compounds and metabolites was achieved with an ACQUITY UPLC BEH C18 column (2.1 mm × 50 mm, 1.7 μm) under a gradient elution. The mobile phase was composed of acetonitrile and water buffered with ammonium acetate (10 mM) and formic acid (0.1%, V/V). Twelve absorbed compounds and four metabolites were found. Seven of the absorbed compounds were identified by ESI-MS. The identification of absorbed compounds might be helpful for the better understanding of the mechanisms underlying the pharmacological effects of WJSH capsule.
authors.affiliation: School of Chemistry & Chemical Engineering, Harbin Normal University, Shi Da Nan Road, Harbin 150025, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Geng
authors.forename: Fashan
authors.initials: F
authors.lastname: Wang
authors.forename: Taobo
authors.initials: T
authors.lastname: Zou
authors.forename: Kuiyuan
authors.initials: K
authors.lastname: Zhu
authors.forename: Wei
authors.initials: W
authors.lastname: Ma
authors.forename: Guanghui
authors.initials: G
authors.lastname: Tan
authors.forename: Ning
authors.initials: N
authors.lastname: Zhang