title: Over-expression of human cytomegalovirus miR-US25-2-3p downregulates eIF4A1 and inhibits HCMV replication.
abstract: It has been reported that human cytomegalovirus (HCMV) miR-US25-2 reduces DNA viral replication including HCMV. However, the mechanism remains unknown. In our study, eukaryotic translation initiation factor 4A1 (eIF4A1) was identified to be a direct target of miR-US25-2-3p. Small interfering RNA (siRNA) and miR-US25-2-3p mediated eIF4A1 knockdown experiments revealed that high level of miR-US25-2-3p in MRC-5 cells decreased HCMV and host genomic DNA synthesis, and inhibited cap-dependent translation and host cell proliferation. However, eIF4A1 up-regulation induced by miR-US25-2-3p inhibitor increased HCMV copy number. Therefore, the over-expression of miR-US25-2-3p and consequent lower expression of eIF4A1 may contribute to the inhibition of HCMV replication.
authors.affiliation: Virus Laboratory, the Affiliated Shengjing Hospital, China Medical University, Shenyang 110004, China.
authors.forename: Manlong
authors.initials: M
authors.lastname: Qi
authors.forename: Ying
authors.initials: Y
authors.lastname: Qi
authors.forename: Yanping
authors.initials: Y
authors.lastname: Ma
authors.forename: Rong
authors.initials: R
authors.lastname: He
authors.forename: Yaohua
authors.initials: Y
authors.lastname: Ji
authors.forename: Zhengrong
authors.initials: Z
authors.lastname: Sun
authors.forename: Qiang
authors.initials: Q
authors.lastname: Ruan
mesh_terms: D001483:Base Sequence; D002460:Cell Line; D049109:Cell Proliferation; D003587:Cytomegalovirus / Q000502:physiology*; D004261:DNA Replication; D015536:Down-Regulation; D039601:Eukaryotic Initiation Factor-4A / Q000235:genetics* / Q000378:metabolism; D015894:Genome, Human; D054884:Host-Pathogen Interactions; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D014176:Protein Biosynthesis; D034622:RNA Interference; D012367:RNA, Viral / Q000235:genetics* / Q000378:metabolism; D014779:Virus Replication
chemical_list: D035683:MicroRNAs; D012367:RNA, Viral; D039601:Eukaryotic Initiation Factor-4A