title: Perinatal and postweaning exposure to galactooligosaccharides/inulin prebiotics induced biomarkers linked to tolerance mechanism in a mouse model of strong allergic sensitization.
abstract: Food allergies are increasing, and no treatment exists, thus enhancing interest in prebiotic strategies. This study aimed to analyze the preventive effects of prebiotic feeding during perinatal and postweaning periods in a mouse model of allergy by studying biomarkers related to tolerance (IgG2a, IgA, IFN-γ, TGF-β, and IL-10), to allergy (IgE, IgG1, IL-4, IL-17, symptoms), and to microbiota (propionate and MyD88). Balb/c mice, both dams and their pups, were fed a diet supplemented with (+Prb) or without (-Prb) GOS/inulin prebiotics. Mice were then sensitized with allergens. Regardless of diet, sensitized mice exhibited similar levels of IgE, IgG1, CD-23, IL-4, IL-17, and symptoms. However, in comparison to -Prb-sensitized mice, +Prb-sensitized mice displayed higher concentrations of total IgG2a (6669 ± 1788 vs 3696 ± 1326 fluorescence units, p < 0.005), specific IgA (285 ± 26 vs 156 ± 9 fluorescence units, p < 0.01), IFN-γ (3194 ± 424 vs 1853 ± 434 pg/mL, p < 0.01), IL-10 (777 ± 87 vs 95 ± 136 pg/mL, p < 0.005), TGF-β (4853 ± 1959 vs 243 ± 444 pg/mL, p < 0.01), MyD88 (0.033 ± 0.019 vs 0.009 ± 0.004 relative expression, p < 0.01), and propionate (4.15 ± 0.8 vs 2.9 ± 1.15 μmol, p < 0.05). In a mouse model of allergy, prebiotic exposure during perinatal and postweaning periods induced the highest expression of biomarkers related to tolerance without affecting biomarkers related to allergy.
authors.affiliation: INRA, UR 1268 BIA, équipe Allergie, rue de la Géraudière, B.P. 71627, F-44316 Nantes Cedex 03, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Gourbeyre
authors.forename: Nicolas
authors.initials: N
authors.lastname: Desbuards
authors.forename: Guilaine
authors.initials: G
authors.lastname: Grémy
authors.forename: Olivier
authors.initials: O
authors.lastname: Tranquet
authors.forename: Martine
authors.initials: M
authors.lastname: Champ
authors.forename: Sandra
authors.initials: S
authors.lastname: Denery-Papini
authors.forename: Marie
authors.initials: M
authors.lastname: Bodinier
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000097:blood; D004195:Disease Models, Animal; D005260:Female; D005697:Galactosides / Q000737:chemistry / Q000627:therapeutic use; D006967:Hypersensitivity / Q000097:blood / Q000276:immunology / Q000517:prevention & control*; D007108:Immune Tolerance; D007444:Inulin / Q000627:therapeutic use*; D039382:Maternal Nutritional Physiological Phenomena; D051379:Mice; D009844:Oligosaccharides / Q000737:chemistry / Q000627:therapeutic use*; D018743:Perinatal Care; D056692:Prebiotics; D014886:Weaning
chemical_list: D015415:Biomarkers; D005697:Galactosides; D009844:Oligosaccharides; D056692:Prebiotics; D007444:Inulin