title: [Genetic transformation of flax (Linum usitatissimum L.) with chimeric GFP-TUA6 gene for visualisation of microtubules].
abstract: The data of Agrobacterium-mediated transformation of some Linum usitatissimum cultivars zoned on the territories of Belarus and Ukraine with the plasmid carrying chimeric GFP-TUA6 gene and nptII gene as selectable marker conferring resistance to kanamycin are presented in this study. Transformation was affected by a number of factors including optical density (OD600), time of inoculation of explants with Agrobacterium and co-culture conditions. Transgenic nature of obtained lines was confirmed by PCR analysis. Expression of GFP-TUA6 gene was detected with confocal laser scanning microscopy. The obtained transgenic lines can be used for further functional studies the role of microtubules in the processes of building the flax fibres and resistance to wind.
authors.forename: E N
authors.initials: EN
authors.lastname: Shisha
authors.forename: V I
authors.initials: VI
authors.lastname: Korkhovoĭ
authors.forename: G Ia
authors.initials: GIa
authors.lastname: Baer
authors.forename: E V
authors.initials: EV
authors.lastname: Guzenko
authors.forename: V A
authors.initials: VA
authors.lastname: Lemesh
authors.forename: N A
authors.initials: NA
authors.lastname: Kartel'
authors.forename: A I
authors.initials: AI
authors.lastname: Emets
authors.forename: Ia B
authors.initials: IaB
authors.lastname: Blium
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D019597:Flax / Q000235:genetics / Q000648:ultrastructure; D017343:Genes, Plant; D049452:Green Fluorescent Proteins / Q000235:genetics*; D018613:Microscopy, Confocal; D008870:Microtubules / Q000235:genetics / Q000648:ultrastructure*; D030821:Plants, Genetically Modified; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins / Q000235:genetics*; D014170:Transformation, Genetic; D014404:Tubulin / Q000235:genetics*
chemical_list: D029681:Arabidopsis Proteins; D018744:DNA, Plant; D011993:Recombinant Fusion Proteins; D014404:Tubulin; D049452:Green Fluorescent Proteins