title: Antinociceptive effects of oxymatrine from Sophora flavescens, through regulation of NR2B-containing NMDA receptor-ERK/CREB signaling in a mice model of neuropathic pain.
abstract: OBJECTIVE
  In this study we investigated antinociceptive effects of oxymatrine through regulation of NR2B-containing NMDA receptor-ERK/CREB signaling in a chronic neuropathic pain model induced by chronic constrictive injury (CCI) of the sciatic nerve.
  
  
  METHODS
  The von Frey and plantar tests were performed to assess the degree of mechanical and thermal changes respectively. Immunohistochemistry assay was used to evaluate the expressions of NR2B. Western blotting assay were used to evaluate the expressions of NR2B, tERK, p-ERK, tCREB and p-CREB.
  
  
  RESULTS
  The intraperitoneal administration of OMT (160, 80 mg/kg) could prevent the development of mechanical allodynia, thermal hyperalgesia induced by CCI. Intraperitoneal administration of OMT decreased the mean IOD of NR2B in the dorsal horn and expression of NR2B, p-ERK and p-CREB protein.
  
  
  CONCLUSIONS
  Regulation of NMDA NR2B receptor-ERK/CREB signaling maybe the targets for the antinociceptive effects of OMT on a chronic neuropathic pain model induced by chronic constrictive injury of the sciatic nerve.
authors.affiliation: Department of Pharmacology, Ningxia Medical University, Yinchuan, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Wang
authors.forename: Yuxiang
authors.initials: Y
authors.lastname: Li
authors.forename: Linglu
authors.initials: L
authors.lastname: Dun
authors.forename: Yaqiong
authors.initials: Y
authors.lastname: Xu
authors.forename: Shaojv
authors.initials: S
authors.lastname: Jin
authors.forename: Juan
authors.initials: J
authors.lastname: Du
authors.forename: Lin
authors.initials: L
authors.lastname: Ma
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.forename: Ru
authors.initials: R
authors.lastname: Zhou
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: He
authors.forename: Tao
authors.initials: T
authors.lastname: Sun
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Yu
mesh_terms: D000470:Alkaloids / Q000494:pharmacology / Q000627:therapeutic use*; D000700:Analgesics / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D059350:Chronic Pain / Q000188:drug therapy / Q000378:metabolism; D003250:Constriction; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D004195:Disease Models, Animal; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D006930:Hyperalgesia / Q000378:metabolism / Q000517:prevention & control; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009437:Neuralgia / Q000188:drug therapy* / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D011807:Quinolizines / Q000494:pharmacology / Q000627:therapeutic use*; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D012584:Sciatic Nerve / Q000187:drug effects* / Q000293:injuries / Q000473:pathology; D015398:Signal Transduction; D029909:Sophora / Q000737:chemistry*; D000093842:Matrines
chemical_list: D000470:Alkaloids; D000700:Analgesics; D017362:Cyclic AMP Response Element-Binding Protein; D048049:Extracellular Signal-Regulated MAP Kinases; D010936:Plant Extracts; D011807:Quinolizines; D016194:Receptors, N-Methyl-D-Aspartate; C121001:NR2B NMDA receptor; C037573:oxymatrine; D000093842:Matrines
keywords: Chronic constrictive injury; NR2B; Neuropathic pain; Oxymatrine from Sophora flavescens; p-CREB; p-ERK