title: Degradation of lindane and endosulfan by fungi, fungal and bacterial laccases.
abstract: The ability of two white-rot fungi (Trametes versicolor and Pleurotus ostreatus) and one brown-rot fungus (Gloeophyllum trabeum) to degrade two organochlorine insecticides, lindane and endosulfan, in liquid cultures was studied and dead fungal biomass was examined for adsorption of both insecticides from liquid medium. Lindane and endosulfan were also treated with fungal laccase and bacterial protein CotA, which has laccase activities. The amount of degraded lindane and endosulfan increased with their exposure period in the liquid cultures of both examined white-rot fungi. Endosulfan was transformed to endosulfan sulphate by T. versicolor and P. ostreatus. A small amount of endosulfan ether was also detected and its origin was examined. Degradation of lindane and endosulfan by a brown rot G. trabeum did not occur. Mycelial biomasses of all examined fungi have been found to adsorb lindane and endosulfan and adsorption onto fungal biomass should therefore be considered as a possible mechanism of pollutant removal when fungal degradation potentials are studied. Bacterial protein CotA performed more efficient degradation of lindane and endosulfan than fungal laccase and has shown potential for bioremediation of organic pollutants.
authors.affiliation: Department of Wood Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1000, Ljubljana, Slovenia, ajda.ulcnik@bf.uni-lj.si.
authors.forename: A
authors.initials: A
authors.lastname: Ulčnik
authors.forename: I
authors.initials: I
authors.lastname: Kralj Cigić
authors.forename: F
authors.initials: F
authors.lastname: Pohleven
mesh_terms: D001426:Bacterial Proteins; D001487:Basidiomycota / Q000201:enzymology; D001673:Biodegradation, Environmental; D018533:Biomass; D001709:Biotechnology; D004726:Endosulfan / Q000031:analogs & derivatives / Q000378:metabolism*; D005656:Fungal Proteins / Q000378:metabolism; D005658:Fungi / Q000201:enzymology*; D001556:Hexachlorocyclohexane / Q000378:metabolism*; D007306:Insecticides / Q000378:metabolism*; D042845:Laccase / Q000378:metabolism*; D020076:Pleurotus / Q000201:enzymology; D055454:Trametes / Q000201:enzymology
chemical_list: D001426:Bacterial Proteins; D005656:Fungal Proteins; D007306:Insecticides; C544690:endosulfan ether; D001556:Hexachlorocyclohexane; C544689:endosulfan sulfate; D042845:Laccase; D004726:Endosulfan