title: Decreased hematopoietic progenitor cell mobilization in pearl mice.
abstract: Neutropenia is common to both Hermansky-Pudlak syndrome type 2 and canine cyclic hematopoiesis (CH) which are caused by mutations in the AP3B1 gene. The purpose of this study was to determine if pearl mice were neutropenic. Complete blood counts (CBCs) and bone marrow differential counts, colony forming unit (CFU) assay, bone marrow lineage negative (lin(-)), Sca(+) and c-kit(+) cells (LSK cells), bone marrow elastase, myeloperoxidase, and cathepsin G enzyme activity were compared in C57Bl6 (Bl/6) and pearl mice. Stress granulopoiesis was evaluated following 200 mg/kg cyclophosphamide or 1 mg/kg bortezomib administration and by limiting dilution bone marrow transplantation. The CBCs and CFUs were determined in Bl/6 and pearl mice following AMD3100 or granulocyte colony-stimulating factor (G-CSF) administration. Pearl mice were not neutropenic and did not have cyclic neutropenia. Bone marrow elastase, myeloperoxidase, and cathepsin G enzyme activity were similar in pearl and Bl/6 mice. The numbers of CFU-G, CFU-GEMM, and LSK cells were increased moderately in pearl mice. Stress granulopoiesis was similar in Bl/6 and pearl mice. CFU assays and CBCs performed on Bl/6 and pearl mice administered AMD3100 resulted in similar results. However, normal mice administered G-CSF had higher peripheral blood neutrophil counts and greater CFU numbers compared with pearl mice. Unlike patients with HPS-2 and dogs with CH, pearl mice did not have neutropenia or CH but had decreased hematopoietic progenitor cell and granulocyte mobilization in response to G-CSF.
authors.affiliation: Department of Biochemistry and Molecular Genetics, University Alabama at Birmingham, Birmingham, Alabama, USA.
authors.forename: Matthew O
authors.initials: MO
authors.lastname: Vallejo
authors.forename: Glenn P
authors.initials: GP
authors.lastname: Niemeyer
authors.forename: Alex
authors.initials: A
authors.lastname: Vaglenov
authors.forename: Tommy
authors.initials: T
authors.lastname: Hock
authors.forename: Bridget
authors.initials: B
authors.lastname: Urie
authors.forename: Peter
authors.initials: P
authors.lastname: Christopherson
authors.forename: Clinton D
authors.initials: CD
authors.lastname: Lothrop
mesh_terms: D000818:Animals; D019380:Anti-HIV Agents / Q000494:pharmacology; D001596:Benzylamines; D001772:Blood Cell Count; D001854:Bone Marrow Cells / Q000201:enzymology; D016026:Bone Marrow Transplantation; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D000080027:Cyclams; D005434:Flow Cytometry; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006098:Granulocytes / Q000166:cytology; D019650:Hematopoietic Stem Cell Mobilization; D006412:Hematopoietic Stem Cells / Q000187:drug effects / Q000473:pathology*; D006571:Heterocyclic Compounds / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D009503:Neutropenia / Q000473:pathology; D010196:Pancreatic Elastase / Q000378:metabolism; D013997:Time Factors
chemical_list: D019380:Anti-HIV Agents; D001596:Benzylamines; D000080027:Cyclams; D006571:Heterocyclic Compounds; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D010196:Pancreatic Elastase; C088327:plerixafor