title: Efficient production of dihydroxyacetone from biodiesel-derived crude glycerol by newly isolated Gluconobacter frateurii.
abstract: The efficient production of dihydroxyacetone (DHA) on biodiesel-derived glycerol based media was developed. A newly isolated strain, Gluconobacter frateurii CGMCC 5397, could convert crude glycerol to DHA with high yield and productivity. In shake-flask fermentation, the DHA concentration of 73.1 gl(-1) was attained at 48 h using an optimum medium containing biodiesel-derived crude glycerol. When fed-batch fermentation was carried out in a 7-l stirred bioreactor with crude glycerol, the DHA concentration, productivity, and yield were 125.8 gl(-1), 2.6 gl(-1)h(-1), and 90.5% at 48 h, respectively. This study suggests that the inexpensive biodiesel-derived crude glycerol could be utilized for efficient production of DHA by G. frateurii.
authors.affiliation: Institute of Bioengineering, School of Life Sciences, Henan University, Kaifeng 475004, PR China.
authors.forename: Yu-Peng
authors.initials: YP
authors.lastname: Liu
authors.forename: Yang
authors.initials: Y
authors.lastname: Sun
authors.forename: Cong
authors.initials: C
authors.lastname: Tan
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Zheng
authors.forename: Kui-Qi
authors.initials: KQ
authors.lastname: Jin
authors.forename: Gang
authors.initials: G
authors.lastname: Wang
mesh_terms: D001483:Base Sequence; D056804:Biofuels; D019149:Bioreactors; D017931:DNA Primers; D004098:Dihydroxyacetone / Q000096:biosynthesis*; D005285:Fermentation; D020576:Gluconobacter / Q000145:classification / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism*; D010802:Phylogeny
chemical_list: D056804:Biofuels; D017931:DNA Primers; D004098:Dihydroxyacetone; D005990:Glycerol
keywords: Biodiesel-derived; Crude glycerol; Dihydroxyacetone; Gluconobacter frateurii