title: The first purification and unequivocal characterization of the radical form of the carbon-centered quinone ketoxy radical adduct.
abstract: We found, unexpectedly, that the radical form of the carbon-centered quinone ketoxy radical adduct with a recently developed spin-trapping agent BMPO can not only be directly detected and identified using HPLC/high resolution MS, but can also be isolated and purified using semi-preparative HPLC, enabling direct observation of its clean 6-line ESR signal.
authors.affiliation: State Key Lab of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Science, CAS, Beijing 100085, China.
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Huang
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Shan
authors.forename: Li
authors.initials: L
authors.lastname: Mao
authors.forename: Balaraman
authors.initials: B
authors.lastname: Kalyanaraman
authors.forename: Hao
authors.initials: H
authors.lastname: Qin
authors.forename: Fu-Rong
authors.initials: FR
authors.lastname: Ren
authors.forename: Ben-Zhan
authors.initials: BZ
authors.lastname: Zhu
mesh_terms: D002244:Carbon / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D003497:Cyclic N-Oxides / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D005609:Free Radicals / Q000737:chemistry*; D013058:Mass Spectrometry; D011809:Quinones / Q000737:chemistry*; D019097:Spin Trapping
chemical_list: C441196:5-tert-butoxycarbonyl 5-methyl-1-pyrroline N-oxide; D003497:Cyclic N-Oxides; D005609:Free Radicals; D011809:Quinones; D002244:Carbon