title: [Value of detecting epidermal growth factor receptor mutations in non-small cell lung cancer tissue by TaqMan-amplification refractory mutation system].
abstract: OBJECTIVE
  To explore the value of detecting mutations on epidermal growth factor receptor (EGFR) gene in non-small cell lung cancer (NSCLC) tissue by TaqMan-amplification refractory mutation system (TaqMan-ARMS).
  
  
  METHODS
  TaqMan-ARMS and DNA sequencing were used to detect the EGFR exon 19 and 21 mutations in tumor tissues and the samples collected from 199 patients at 4 different 3A hospitals in Beijing from January 2008 to March 2011.
  
  
  RESULTS
  The rate of mutations in EGFR exon 19 and 21 was 19.1% (38/199), according to their different pathological types. Based upon TaqMan-ARMS, the classification was as followed: adenocarcinoma (35.0% (36/103)), squamous carcinoma (2.2% (2/93)) and adenosquamous carcinoma (0). According to DNA sequencing, they were 19.6% (39/199), 35.9% (37/103), 2.2% (2/93) and 0 respectively. Thus, no statistically significant difference existed between two methods (McNemar Test, P = 1.000, κ = 0.984). The mutation rate of adenocarcinoma was higher than those of squamous and adenosquamous carcinoma.
  
  
  CONCLUSIONS
  The detection of EGFR mutations is highly consistent in the NSCLC tissue by the methods of TaqMan-ARMS and DNA sequencing.
authors.affiliation: Chinese Academy of Medical Sciences, Beijing, China.
authors.forename: Ya-ling
authors.initials: YL
authors.lastname: Dou
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Qi
authors.forename: Lun
authors.initials: L
authors.lastname: Xin
authors.forename: Dong
authors.initials: D
authors.lastname: Chen
authors.forename: Hai-qing
authors.initials: HQ
authors.lastname: Zhang
authors.forename: Quan-cai
authors.initials: QC
authors.lastname: Cui
mesh_terms: D000328:Adult; D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics*; D004252:DNA Mutational Analysis; D066246:ErbB Receptors / Q000235:genetics*; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009154:Mutation; D021141:Nucleic Acid Amplification Techniques
chemical_list: D066246:ErbB Receptors