title: [The mechanism of PINK1 localization on the outer membrane of mitochondria].
abstract: OBJECTIVE
  To study the specific mechanism of PTEM-induced putative kinase 1(PINK1) located to the outer membrane of damaged mitochondria.
  
  
  METHODS
  Cultured HEK293T cells were transfected with plasmids expressing different proteins, following with DMSO or CCCP treatment. Western blot and coimmuoprecipitation were used to detect the expression and interaction of proteins.
  
  
  RESULTS
  Full length PINK1, but not its mitochondria targeting sequence (MTS) & trans-membrane (TM) deleted forms or other outer mitochondria outer membrane proteins, could interact with Tom40 upon CCCP treatment and the interaction ability was more than 20 times stronger than that of DMSO control. When the added CCCP is washed out, the interaction between full length PINK1 and Tom40 declined rapidly. PINK1 with removed or mutated TM can interact with Tom40 even in the absence of CCCP.
  
  
  CONCLUSIONS
  The accumulated PINK1 on the outer membrane of damaged mitochondria is just stuck on the TOM complex instead of integrated into the lipid bilayer.
authors.affiliation: Developmental & Stem Cell Institute, West China Second Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Hao-Ran
authors.initials: HR
authors.lastname: Tai
authors.forename: Chang-An
authors.initials: CA
authors.lastname: Jiang
mesh_terms: D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone / Q000494:pharmacology; D057809:HEK293 Cells; D006801:Humans; D008928:Mitochondria / Q000201:enzymology*; D033681:Mitochondrial Membrane Transport Proteins / Q000378:metabolism; D051336:Mitochondrial Membranes / Q000201:enzymology*; D010300:Parkinson Disease / Q000235:genetics* / Q000378:metabolism; D011494:Protein Kinases / Q000235:genetics* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D000099202:PTEN-Induced Putative Kinase
chemical_list: D002258:Carbonyl Cyanide m-Chlorophenyl Hydrazone; D033681:Mitochondrial Membrane Transport Proteins; D011494:Protein Kinases; D029701:Saccharomyces cerevisiae Proteins; D000099202:PTEN-Induced Putative Kinase; C066555:Tom40 protein, S cerevisiae