title: Genome-wide association study and pathway-level analysis of tocochromanol levels in maize grain.
abstract: Tocopherols and tocotrienols, collectively known as tocochromanols, are the major lipid-soluble antioxidants in maize (Zea mays L.) grain. Given that individual tocochromanols differ in their degree of vitamin E activity, variation for tocochromanol composition and content in grain from among diverse maize inbred lines has important nutritional and health implications for enhancing the vitamin E and antioxidant contents of maize-derived foods through plant breeding. Toward this end, we conducted a genome-wide association study of six tocochromanol compounds and 14 of their sums, ratios, and proportions with a 281 maize inbred association panel that was genotyped for 591,822 SNP markers. In addition to providing further insight into the association between ZmVTE4 (γ-tocopherol methyltransferase) haplotypes and α-tocopherol content, we also detected a novel association between ZmVTE1 (tocopherol cyclase) and tocotrienol composition. In a pathway-level analysis, we assessed the genetic contribution of 60 a priori candidate genes encoding the core tocochromanol pathway (VTE genes) and reactions for pathways supplying the isoprenoid tail and aromatic head group of tocochromanols. This analysis identified two additional genes, ZmHGGT1 (homogentisate geranylgeranyltransferase) and one prephenate dehydratase parolog (of four in the genome) that also modestly contribute to tocotrienol variation in the panel. Collectively, our results provide the most favorable ZmVTE4 haplotype and suggest three new gene targets for increasing vitamin E and antioxidant levels through marker-assisted selection.
authors.affiliation: United States Department of Agriculture-Agricultural Research Service, Robert W. Holley Center for Agriculture and Health, Ithaca, New York 14853, USA.
authors.forename: Alexander E
authors.initials: AE
authors.lastname: Lipka
authors.forename: Michael A
authors.initials: MA
authors.lastname: Gore
authors.forename: Maria
authors.initials: M
authors.lastname: Magallanes-Lundback
authors.forename: Alex
authors.initials: A
authors.lastname: Mesberg
authors.forename: Haining
authors.initials: H
authors.lastname: Lin
authors.forename: Tyler
authors.initials: T
authors.lastname: Tiede
authors.forename: Charles
authors.initials: C
authors.lastname: Chen
authors.forename: C Robin
authors.initials: CR
authors.lastname: Buell
authors.forename: Edward S
authors.initials: ES
authors.lastname: Buckler
authors.forename: Torbert
authors.initials: T
authors.lastname: Rocheford
authors.forename: Dean
authors.initials: D
authors.lastname: DellaPenna
mesh_terms: D019883:Alkyl and Aryl Transferases / Q000235:genetics / Q000378:metabolism; D055106:Genome-Wide Association Study; D005838:Genotype; D006239:Haplotypes; D019751:Intramolecular Transferases / Q000235:genetics / Q000378:metabolism; D015810:Linkage Disequilibrium; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide; D024505:Tocopherols / Q000378:metabolism*; D024508:Tocotrienols / Q000378:metabolism*; D003313:Zea mays / Q000235:genetics* / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D024508:Tocotrienols; D008780:Methyltransferases; C047259:gamma-tocopherol methyltransferase; D019883:Alkyl and Aryl Transferases; D019751:Intramolecular Transferases; C103397:tocopherol cyclase; D024505:Tocopherols
keywords: GWAS; biofortification; candidate gene; vitamin E