title: 15N-permethylated amino acids as efficient probes for MRI-DNP applications.
abstract: The synthesis, NMR properties and preliminary polarization tests on protonated and perdeuterated forms of α-trimethylglutamine (NMe3Gln), α-trimethylglutamate (NMe3Glu) and ε-trimethyllysine (NMe3Lys) are reported. The (15)N-permethylated, perdeuterated amino acids display very long (15)N-T1 values, ranging between 190 and 330 s, are well polarized by the dynamic nuclear polarization (DNP) procedure, yielding good polarization levels (10%), and appear to be well tolerated by cells and mice. The obtained results make perdeuterated amino acids excellent candidates for innovative DNP (15)N-MRI applications such as perfusion or targeting studies.
authors.affiliation: Department of Chemistry and Center for Molecular Imaging, University of Turin, Via Nizza 52, 10125 Torino, Italy.
authors.forename: Enrico
authors.initials: E
authors.lastname: Chiavazza
authors.forename: Alessandra
authors.initials: A
authors.lastname: Viale
authors.forename: Magnus
authors.initials: M
authors.lastname: Karlsson
authors.forename: Silvio
authors.initials: S
authors.lastname: Aime
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D000818:Animals; D058948:Cell Tracking; D008279:Magnetic Resonance Imaging; D051379:Mice; D009587:Nitrogen Isotopes / Q000737:chemistry*; D013194:Staining and Labeling
chemical_list: D000596:Amino Acids; D009587:Nitrogen Isotopes