title: Measurement of protein-ligand complex formation.
abstract: Experimental approaches to detect, measure, and quantify protein-ligand binding, along with their theoretical bases, are described. A range of methods for detection of protein-ligand interactions is summarized. Specific protocols are provided for a nonequilibrium procedure pull-down assay, for an equilibrium direct binding method and its modification into a competition-based measurement and for steady-state measurements based on the effects of ligands on enzyme catalysis.
authors.affiliation: Biomolecular Interactions Consultancy, Hertford, UK.
authors.forename: Peter N
authors.initials: PN
authors.lastname: Lowe
authors.forename: Cara K
authors.initials: CK
authors.lastname: Vaughan
authors.forename: Tina
authors.initials: T
authors.lastname: Daviter
mesh_terms: D001345:Autoradiography; D001665:Binding Sites; D001667:Binding, Competitive; D002384:Catalysis; D031541:Fluorescence Resonance Energy Transfer; D006801:Humans; D007700:Kinetics; D008024:Ligands; D008163:Luminescent Measurements; D056004:Molecular Dynamics Simulation; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D008024:Ligands; D011506:Proteins