title: Ciliated micropillars for the microfluidic-based isolation of nanoscale lipid vesicles.
abstract: We fabricated a microfluidic device consisting of ciliated micropillars, forming a porous silicon nanowire-on-micropillar structure. We demonstrated that the prototype device can preferentially trap exosome-like lipid vesicles, while simultaneously filtering out proteins and cell debris. Trapped lipid vesicles can be recovered intact by dissolving the porous nanowires in PBS buffer.
authors.affiliation: Department of Nanomedicine, The Methodist Hospital Research Institute, Houston, Texas, USA.
authors.forename: Zongxing
authors.initials: Z
authors.lastname: Wang
authors.forename: Hung-jen
authors.initials: HJ
authors.lastname: Wu
authors.forename: Daniel
authors.initials: D
authors.lastname: Fine
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Schmulen
authors.forename: Ye
authors.initials: Y
authors.lastname: Hu
authors.forename: Biana
authors.initials: B
authors.lastname: Godin
authors.forename: John X J
authors.initials: JX
authors.lastname: Zhang
authors.forename: Xuewu
authors.initials: X
authors.lastname: Liu
mesh_terms: D004867:Equipment Design; D005374:Filtration / Q000295:instrumentation; D008055:Lipids / Q000302:isolation & purification*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D053770:Nanowires / Q000737:chemistry; D016062:Porosity; D012825:Silicon / Q000737:chemistry
chemical_list: D008055:Lipids; D012825:Silicon