title: [CD44 regulates epithelial-mesenchymal transition and metastasis in nasopharyngeal cancer cells].
abstract: OBJECTIVE
  To study the correlation of CD44 with epithelial-mesenchymal transition(EMT) and metastasis in nasopharyngeal cancer cells, and explore the possible mechanism of CD44 regulates EMT and metastasis in nasopharyngeal cancer cells.
  
  
  METHODS
  The CD44 and EMT-associated proteins in 5-8F and 6-10B nasopharyngeal cancer cell lines were assayed by Western blotting. The erasion trace test was performed to observe the migratory ability of 5-8F and 6-10B nasopharyngeal cancer cells. Using lipid-mediated DNA transfection technique, the low metastatic nasopharyngeal cancer cells 6-10B were transfected in vitro with plasmid which contained CD44 gene, and then new nasopharyngeal cancer cells were obtained. The CD44 and EMT-associated proteins in 6-10B, empty vector transfected and CD44-transfected cells were assayed by Western blotting. The erasion trace test was performed to observe the alteration of migratory ability of nasopharyngeal cancer cells before and after CD44 transfection.
  
  
  RESULTS
  The expression of CD44 and EMT-associated protein MMP-9 in 5-8F was higher than that in 6-10B, but EMT-associated protein E-Cadherin in 5-8F was lower than that in 6-10B. The migratory ability of 5-8F was higher than that of 6-10B. The expression of CD44 and MMP-9 were significantly higher in the CD44-transfected nasopharyngeal cancer cells than in the control groups. Compared with control groups, the migratory ability of CD44-transfected nasopharyngeal cancer cells was significantly increased.
  
  
  CONCLUSIONS
  CD44 positively regulates the metastatic ability of nasopharyngeal cancer cells, which is relevant to the process of EMT.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, the Second Xiangya Hospital, Central South University, Changsha, 410011, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Wang
authors.forename: Shisheng
authors.initials: S
authors.lastname: Li
authors.forename: Dinghua
authors.initials: D
authors.lastname: Xie
authors.forename: Qinglai
authors.initials: Q
authors.lastname: Tang
authors.forename: Shuhui
authors.initials: S
authors.lastname: Wang
authors.forename: Jiajia
authors.initials: J
authors.lastname: Liu
authors.forename: Yuehong
authors.initials: Y
authors.lastname: Chen
authors.forename: Xinming
authors.initials: X
authors.lastname: Yang
mesh_terms: D002277:Carcinoma; D045744:Cell Line, Tumor; D058750:Epithelial-Mesenchymal Transition; D006801:Humans; D018960:Hyaluronan Receptors / Q000235:genetics / Q000378:metabolism*; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism / Q000473:pathology*; D009362:Neoplasm Metastasis; D014162:Transfection
chemical_list: D018960:Hyaluronan Receptors