title: Detection of myo-inositol tris pyrophosphate (ITPP) in equine following an administration of ITPP.
abstract: Myo-Inositol tris pyrophosphate (ITPP) is a powerful allosteric modulator of haemoglobin that increases oxygen-releasing capacity of red blood cells. It is capable of crossing the red blood cell membrane unlike its open polyphosphate analog myo-inositol hexakisphosphate (IHP). Systemic administration of ITPP enhanced the exercise capacity in mice. There have been rumours of its abuse in the horse racing industry to enhance the performance of racing horses. In this paper, the detection of ITPP in equine plasma and urine after an administration of ITPP is reported. A Standardbred mare was administered 200 mg of ITPP intravenously. Urine and plasma samples were collected up to 120 h post administration and analyzed for ITPP by liquid chromatography-tandem mass spectrometry. ITPP was detected in post administration plasma samples up to 6 hours. The peak concentration was detected at 5 min post administration. In urine, ITPP was detected up to 24 h post administration. The peak concentration was detected at 1.5 h post administration.
authors.affiliation: Maxxam Analytics Inc., 8577 Commerce Court, Burnaby, BC, V5A 4N5, Canada.
authors.forename: Geoffrey
authors.initials: G
authors.lastname: Lam
authors.forename: Sarah
authors.initials: S
authors.lastname: Zhao
authors.forename: Jasmeet
authors.initials: J
authors.lastname: Sandhu
authors.forename: Rong
authors.initials: R
authors.lastname: Yi
authors.forename: Devan
authors.initials: D
authors.lastname: Loganathan
authors.forename: Barbara
authors.initials: B
authors.lastname: Morrissey
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006736:Horses / Q000097:blood* / Q000652:urine*; D007295:Inositol Phosphates / Q000008:administration & dosage / Q000097:blood* / Q000652:urine*; D057230:Limit of Detection; D015813:Substance Abuse Detection / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D007295:Inositol Phosphates; C516441:inositol trispyrophosphate
keywords: HILIC; HPLC-MS-MS; ITPP; equine; intravenous administration; plasma; urine