title: Optical detection of epigenetic marks: sensitive quantification and direct imaging of individual hydroxymethylcytosine bases.
abstract: Herein we report the specific labelling of the epigenetic modification 5-hydroxymethyl-cytosine along genomic DNA molecules with a fluorescent reporter molecule. Enzymatic glucosylation followed by a click chemistry reaction enables single molecule detection as well as global quantification of 5hmC in genomic DNA.
authors.affiliation: Raymond and Beverly Sackler Faculty of Exact Sciences, School of Chemistry, Tel Aviv University, Tel Aviv, Israel. uv@post.tau.ac.il.
authors.forename: Yael
authors.initials: Y
authors.lastname: Michaeli
authors.forename: Tamar
authors.initials: T
authors.lastname: Shahal
authors.forename: Dmitry
authors.initials: D
authors.lastname: Torchinsky
authors.forename: Assaf
authors.initials: A
authors.lastname: Grunwald
authors.forename: Rony
authors.initials: R
authors.lastname: Hoch
authors.forename: Yuval
authors.initials: Y
authors.lastname: Ebenstein
mesh_terms: D044503:5-Methylcytosine / Q000031:analogs & derivatives; D000818:Animals; D010582:Bacteriophage lambda / Q000737:chemistry; D003596:Cytosine / Q000031:analogs & derivatives* / Q000032:analysis; D004247:DNA / Q000737:chemistry*; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D005456:Fluorescent Dyes / Q000032:analysis; D051379:Mice; D008958:Models, Molecular; D061848:Optical Imaging
chemical_list: D005456:Fluorescent Dyes; C011865:5-hydroxymethylcytosine; D044503:5-Methylcytosine; D003596:Cytosine; D004247:DNA