title: Magnetic resonance imaging and tracking of stem cells.
abstract: To date, several stem cell labeling protocols have been developed, contributing to a fast growing and promising field of stem cell imaging by MRI (magnetic resonance imaging). Most of these methods utilize iron oxide nanoparticles (MION, SPIO, USPIO, VSIOP) for cell labeling, which provide negative (dark) signal effects on T2-weighted MR images. The following protocol describes stem cell labeling techniques with commercially available gadolinium chelates, which provide positive contrast on T1-weighted MR images, which can be advantageous for specific applications.
authors.affiliation: Molecular Imaging Program at Stanford (MIPS), Department of Radiology, Stanford School of Medicine, Stanford University, 265 Campus Drive, Room G2045, Lokey Stem Cell Research Building, Stanford, CA 94305, USA. Hossein_Nejadnik@stanford.edu
authors.forename: Hossein
authors.initials: H
authors.lastname: Nejadnik
authors.forename: Rostislav
authors.initials: R
authors.lastname: Castillo
authors.forename: Heike E
authors.initials: HE
authors.lastname: Daldrup-Link
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology; D058948:Cell Tracking / Q000379:methods*; D002478:Cells, Cultured; D005682:Gadolinium; D006571:Heterocyclic Compounds; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells; D009942:Organometallic Compounds; D013194:Staining and Labeling
chemical_list: D006571:Heterocyclic Compounds; D009942:Organometallic Compounds; C062402:gadoteridol; D005682:Gadolinium