title: Resident CD8(+) and migratory CD103(+) dendritic cells control CD8 T cell immunity during acute influenza infection.
abstract: The identification of the specific DC subsets providing a critical role in presenting influenza antigens to naïve T cell precursors remains contentious and under considerable debate. Here we show that CD8(+) T lymphocyte (TCD8+) responses are severely hampered in C57BL/6 mice deficient in the transcription factor Batf3 after intranasal challenge with influenza A virus (IAV). This transcription factor is required for the development of lymph node resident CD8(+) and migratory CD103(+)CD11b(-) DCs and we found both of these subtypes could efficiently stimulate anti-IAV TCD8+. Using a similar ex vivo approach, many publications on this subject matter excluded a role for resident, non-migratory CD8(+) DC. We postulate the differences reported can partially be explained by how DC are phenotyped, namely the use of MHC class II to segregate subtypes. Our results show that resident CD8(+) DC upregulate this marker during IAV infection and we advise against its use when isolating DC subtypes.
authors.affiliation: Telethon Institute for Child Health Research and Centre for Child Health Research, University of Western Australia, Perth, Western Australia, Australia. jwaithman@ichr.uwa.edu.au
authors.forename: Jason
authors.initials: J
authors.lastname: Waithman
authors.forename: Damien
authors.initials: D
authors.lastname: Zanker
authors.forename: Kun
authors.initials: K
authors.lastname: Xiao
authors.forename: Sara
authors.initials: S
authors.lastname: Oveissi
authors.forename: Ben
authors.initials: B
authors.lastname: Wylie
authors.forename: Royce
authors.initials: R
authors.lastname: Ng
authors.forename: Lars
authors.initials: L
authors.lastname: Tögel
authors.forename: Weisan
authors.initials: W
authors.lastname: Chen
mesh_terms: D000208:Acute Disease; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002465:Cell Movement / Q000276:immunology*; D003713:Dendritic Cells / Q000166:cytology* / Q000276:immunology / Q000378:metabolism*; D005260:Female; D006801:Humans; D009980:Influenza A virus / Q000502:physiology*; D039001:Integrin alpha Chains / Q000378:metabolism*; D051379:Mice; D009976:Orthomyxoviridae Infections / Q000276:immunology*
chemical_list: D015703:Antigens, CD; D039001:Integrin alpha Chains; C091367:alpha E integrins