title: New perspective on the stabilization and degradation of the F-box protein COI1 in Arabidopsis.
abstract: The F-box protein CORONATINE INSENSITIVE1 (COI1) assembles into SCF(COI1) complexes and recruits its substrate JAZ proteins for ubiquitination and degradation to regulate diverse aspects of jasmonate-regulated plant developmental processes and defense responses. However, the dynamically regulation of COI1 protein abundance in plants remains unknown. In our Plant Cell paper, through genetic, biochemical analysis and in vitro degradation assays, we demonstrated that the COI1 protein is strictly regulated by a dynamic balance of SCF(COI1)-mediated stabilization and 26S proteasome-mediated degradation, and maintained at a proper level suitable for essential biological processes in plants. In this addendum, we provided additional insights and speculation on the stabilization and degradation of COI1.
authors.affiliation: Tsinghua-Peking Center for Life Sciences; MOE Key Laboratory of Bioinformatics; School of Life Sciences; Tsinghua University; Beijing, P.R. China.
authors.forename: Wu
authors.initials: W
authors.lastname: Zhou
authors.forename: Ruifeng
authors.initials: R
authors.lastname: Yao
authors.forename: Haiou
authors.initials: H
authors.lastname: Li
authors.forename: Suhua
authors.initials: S
authors.lastname: Li
authors.forename: Jianbin
authors.initials: J
authors.lastname: Yan
mesh_terms: D019943:Amino Acid Substitution; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D044783:F-Box Proteins / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D010802:Phylogeny; D030821:Plants, Genetically Modified; D055550:Protein Stability; D059748:Proteolysis
chemical_list: C120363:ASK1 protein, Arabidopsis; D029681:Arabidopsis Proteins; C112279:COI1 protein, Arabidopsis; D044783:F-Box Proteins
keywords: ASK1; ASK2; COI1; SCF; jasmonate; protein stabilization; ubiquitination