title: Evaluation of a novel real-time RT-PCR using TOCE technology compared with culture and Seeplex RV15 for simultaneous detection of respiratory viruses.
abstract: BACKGROUND
  Various kinds of commercial molecular systems have been developed for fast and more accurate detection of respiratory viruses. Anyplex™ II RV16 [RV16] was designed for simultaneous detection of 16 respiratory viruses using multiplex PCR coupled with TOCE™ technology.
  
  
  OBJECTIVE
  To compare the performance of RV16 with those of culture and Seeplex(®) RV15 ACE [RV15] by determining their sensitivity and specificity.
  
  
  METHODS
  Seven hundred and thirty respiratory samples were tested by modified shell vial culture method, RV16, and RV15. For molecular tests, automated nucleic acid extraction and liquid handling system using MICROLAB Nimbus IVD (Hamilton, USA) was adopted to maximize the workflow and accuracy. Performance of each assay was determined against a composite reference standard.
  
  
  RESULTS
  Two hundred and one samples (28%) out of 730 samples were positive by culture, while additional 281 (39%) were positive by RV16 or RV15. Sensitivities of RV16, RV15, and culture for virus tested were as follows: 100/93/63% for influenza A, 90/80/69% for influenza B, 98/94/63% for RSV, 98/52/23% for adenovirus, and 100/75/46% for PIV. For viruses not covered by culture, sensitivities of RV16 and RV15 were as follows: 99/81% for rhinovirus, 92/100% for coronavirus OC43, 100/56% for coronavirus 229E/NL63, 92/88% for metapneumovirus, 100/62% for bocavirus, and 91/91% for enterovirus. Overall, the specificities of culture, RV16, and RV15 (Seegene) were 100/99.9/99.9%.
  
  
  CONCLUSIONS
  RV16 assay was superior to culture method and RV15 and will be a promising tool for patient management and public health epidemiology.
authors.affiliation: Department of Laboratory Medicine, College of Medicine, Korea University, Seoul, Republic of Korea.
authors.forename: Chi Hyun
authors.initials: CH
authors.lastname: Cho
authors.forename: Bayarjavkhlan
authors.initials: B
authors.lastname: Chulten
authors.forename: Chang Kyu
authors.initials: CK
authors.lastname: Lee
authors.forename: Myung Hyun
authors.initials: MH
authors.lastname: Nam
authors.forename: Soo Young
authors.initials: SY
authors.lastname: Yoon
authors.forename: Chae Seung
authors.initials: CS
authors.lastname: Lim
authors.forename: Yunjung
authors.initials: Y
authors.lastname: Cho
authors.forename: Young Kee
authors.initials: YK
authors.lastname: Kim
mesh_terms: D002478:Cells, Cultured; D006801:Humans; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012141:Respiratory Tract Infections / Q000175:diagnosis / Q000821:virology*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014780:Viruses / Q000235:genetics* / Q000302:isolation & purification*