title: Glutathione S-transferases interact with AMP-activated protein kinase: evidence for S-glutathionylation and activation in vitro.
abstract: AMP-activated protein kinase (AMPK) is a cellular and whole body energy sensor with manifold functions in regulating energy homeostasis, cell morphology and proliferation in health and disease. Here we apply multiple, complementary in vitro and in vivo interaction assays to identify several isoforms of glutathione S-transferase (GST) as direct AMPK binding partners: Pi-family member rat GSTP1 and Mu-family members rat GSTM1, as well as Schistosoma japonicum GST. GST/AMPK interaction is direct and involves the N-terminal domain of the AMPK β-subunit. Complex formation of the mammalian GSTP1 and -M1 with AMPK leads to their enzymatic activation and in turn facilitates glutathionylation and activation of AMPK in vitro. GST-facilitated S-glutathionylation of AMPK may be involved in rapid, full activation of the kinase under mildly oxidative physiological conditions.
authors.affiliation: Université Grenoble Alpes, Laboratory of Fundamental and Applied Bioenergetics, Grenoble, France.
authors.forename: Anna
authors.initials: A
authors.lastname: Klaus
authors.forename: Sarah
authors.initials: S
authors.lastname: Zorman
authors.forename: Alexandre
authors.initials: A
authors.lastname: Berthier
authors.forename: Cécile
authors.initials: C
authors.lastname: Polge
authors.forename: Sacnicte
authors.initials: S
authors.lastname: Ramirez
authors.forename: Sylvie
authors.initials: S
authors.lastname: Michelland
authors.forename: Michel
authors.initials: M
authors.lastname: Sève
authors.forename: Didier
authors.initials: D
authors.lastname: Vertommen
authors.forename: Mark
authors.initials: M
authors.lastname: Rider
authors.forename: Nicolas
authors.initials: N
authors.lastname: Lentze
authors.forename: Daniel
authors.initials: D
authors.lastname: Auerbach
authors.forename: Uwe
authors.initials: U
authors.lastname: Schlattner
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D004734:Energy Metabolism / Q000235:genetics*; D004789:Enzyme Activation; D015870:Gene Expression; D005978:Glutathione / Q000378:metabolism*; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism*; D015801:Helminth Proteins / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000737:chemistry / Q000201:enzymology; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012549:Schistosoma japonicum / Q000737:chemistry / Q000201:enzymology; D015398:Signal Transduction
chemical_list: D055372:AMP-Activated Protein Kinases; D005978:Glutathione; D005982:Glutathione Transferase; D015801:Helminth Proteins; D007527:Isoenzymes; D021122:Protein Subunits; D011994:Recombinant Proteins