title: Increased CD45RO+ CD62L+ CD4+ T-cell subpopulation responsible for Th2 response in Kimura's disease.
abstract: Kimura's disease is characterized by subcutaneous masses, eosinophilia, and markedly elevated serum immunoglobulin E, suggesting that T helper (Th)2 cells may play a role in the pathogenesis. We investigated Th2 cytokine synthesis by mononuclear cells and possible Th1/Th2 subpopulations in Kimura's disease. Peripheral blood samples were obtained from seven patients with Kimura's disease and CD4(+) T-cell subpopulations separated by CD45RO and CD62L were isolated. Purified cells were stimulated with PHA or anti-CD3 mAb, and the cytokine levels were measured by Cytometric Bead Array kit. Peripheral blood mononuclear cells in the majority of the patients produced Th2 cytokines such as interleukin (IL)-3, IL-4, IL-5, IL-13 or GM-CSF higher than those of controls. The ratio of CD45RO(+) CD62L(+) cells in CD4(+) T cells was increased in six out of seven patients compared to age-matched controls. Especially, patient 1 had remarkably increased levels of CD45RO(+) CD62L(+) population in CD4(+) T cells. In addition, IL-4 production levels by CD45RO(+) CD62L(+) CD4(+) T cells of patients 1 and 2 were higher than those of their CD45RO(+) CD62L(-) CD4(+) T cells, in the same manner as those by a normal control. Taken together, the synthesis of Th2 cytokines and CD62L-positive subpopulation in CD45RO(+) CD4(+) T cells, which may represent characteristics of Th2, are increased in patients with Kimura's disease, suggesting that deviation to Th2 may involve in pathogenesis of the disease.
authors.affiliation: Department of Infection and Host Defense, Graduate School of Medicine, Shinshu University, Matsumoto, Japan.
authors.forename: Kazuko
authors.initials: K
authors.lastname: Yamazaki
authors.forename: Hisashi
authors.initials: H
authors.lastname: Kawashima
authors.forename: Satoshi
authors.initials: S
authors.lastname: Sato
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tsunoda
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Yoshimura
authors.forename: Makoto
authors.initials: M
authors.lastname: Higuchi
authors.forename: Sho
authors.initials: S
authors.lastname: Hokibara
authors.forename: Takashi
authors.initials: T
authors.lastname: Yamazaki
authors.forename: Kazunaga
authors.initials: K
authors.lastname: Agematsu
mesh_terms: D000293:Adolescent; D000328:Adult; D000796:Angiolymphoid Hyperplasia with Eosinophilia / Q000276:immunology*; D015704:CD4 Antigens / Q000378:metabolism; D002478:Cells, Cultured; D002648:Child; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D006801:Humans; D007073:Immunoglobulin E / Q000097:blood; D019041:L-Selectin / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008213:Lymphocyte Activation; D008297:Male; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*; D055815:Young Adult
chemical_list: D015704:CD4 Antigens; D016207:Cytokines; D019041:L-Selectin; D007073:Immunoglobulin E; D017493:Leukocyte Common Antigens