title: Gene panel model predictive of outcome in patients with prostate cancer.
abstract: In men at high risk for prostate cancer, established clinical and pathological parameters provide only limited prognostic information. Here we analyzed a French cohort of 103 prostate cancer patients and developed a gene panel model predictive of outcome in this group of patients. The model comprised of a 15-gene TaqMan Low-Density Array (TLDA) card, with gene expressions compared to a standardized reference. The RQ value for each gene was calculated, and a scoring system was developed. Summing all the binary scores (0 or 1) corresponding to the 15 genes, a global score is obtained between 0 and 15. This global score can be compared to Gleason score (0 to 10) by recalculating it into a 0-10 scaled score. A scaled score ≥2 suggested that the patient is suffering from a prostate cancer, and a scaled score ≥7 flagged aggressive cancer. Statistical analyses demonstrated a strongly significant linear correlation (p=3.50E-08) between scaled score and Gleason score for this prostate cancer cohort (N=103). These results support the capacity of this designed 15 target gene TLDA card approach to predict outcome in prostate cancer, opening up a new avenue for personalized medicine through future independent replication and applications for rapid identification of aggressive prostate cancer phenotypes for early intervention.
authors.affiliation: Department of Oncogenetics, Centre Jean Perrin, Clermont-Ferrand, France.
authors.forename: Nadège
authors.initials: N
authors.lastname: Rabiau
authors.forename: Yann
authors.initials: Y
authors.lastname: Dantal
authors.forename: Laurent
authors.initials: L
authors.lastname: Guy
authors.forename: Marjolaine
authors.initials: M
authors.lastname: Ngollo
authors.forename: Aslihan
authors.initials: A
authors.lastname: Dagdemir
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Kemeny
authors.forename: Benoît
authors.initials: B
authors.lastname: Terris
authors.forename: Annick
authors.initials: A
authors.lastname: Vieillefond
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Boiteux
authors.forename: Yves-Jean
authors.initials: YJ
authors.lastname: Bignon
authors.forename: Dominique
authors.initials: D
authors.lastname: Bernard-Gallon
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D023281:Genomics; D006801:Humans; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D011379:Prognosis; D011467:Prostate / Q000473:pathology; D011471:Prostatic Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology*