title: Multiparametric evaluation of the cytoprotective effect of the Mangifera indica L. stem bark extract and mangiferin in HepG2 cells.
abstract: OBJECTIVE
  Mango (Mangifera indica L.) stem bark extract (MSBE) is a natural product with biological properties and mangiferin is the major component. This paper reported the evaluation of the protective effects of MSBE and mangiferin against the toxicity induced in HepG2 cells by tert-butyl hydroperoxide or amiodarone.
  
  
  METHODS
  Nuclear morphology, cell viability, intracellular calcium concentration and reactive oxygen species (ROS) production were measured by using a high-content screening multiparametric assay.
  
  
  RESULTS
  MSBE and mangiferin produced no toxicity below 500 mg/ml doses. A marked recovery in cell viability, which was reduced by the toxicants, was observed in cells pre-exposed to MSBE or mangiferin at 5-100 mg/ml doses. We also explored the possible interaction of both products over P-glycoprotein (P-gp). MSBE and mangiferin above 100 mg/ml inhibited the activity of P-gp in HepG2 cells.
  
  
  CONCLUSIONS
  MSBE and mangiferin showed cytoprotective effects of against oxidative damage and mitochondrial toxicity induced by xenobiotics to human hepatic cells but it seemed that other constituents of the extract could contribute to MSBE protective properties. In addition, the drug efflux should be taken into account because of the inhibition of the P-gp function observed in those cells exposed to both natural products.
authors.affiliation: Unidad de Hepatología Experimental, Centro de Investigación, Hospital La Fe, Valencia, Spain.
authors.forename: Laia
authors.initials: L
authors.lastname: Tolosa
authors.forename: Idania
authors.initials: I
authors.lastname: Rodeiro
authors.forename: M Teresa
authors.initials: MT
authors.lastname: Donato
authors.forename: José A
authors.initials: JA
authors.lastname: Herrera
authors.forename: René
authors.initials: R
authors.lastname: Delgado
authors.forename: José V
authors.initials: JV
authors.lastname: Castell
authors.forename: M José
authors.initials: MJ
authors.lastname: Gómez-Lechón
mesh_terms: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism*; D000638:Amiodarone / Q000633:toxicity; D002118:Calcium / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D056945:Hep G2 Cells; D006801:Humans; D031022:Mangifera; D024301:Plant Bark; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000633:toxicity; D018547:Plant Stems; D017382:Reactive Oxygen Species / Q000378:metabolism; D044004:Xanthones / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D020122:tert-Butylhydroperoxide / Q000633:toxicity
chemical_list: D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; C501277:Mangifera indica extract; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D044004:Xanthones; C013592:mangiferin; D020122:tert-Butylhydroperoxide; D000638:Amiodarone; D002118:Calcium