title: Initiation of meiotic recombination in chromatin structure.
abstract: Meiotic homologous recombination is markedly activated during meiotic prophase to play central roles in faithful chromosome segregation and conferring genetic diversity to gametes. It is initiated by programmed DNA double-strand breaks (DSBs) by the conserved protein Spo11, and preferentially occurs at discrete sites called hotspots. Since the functions of Spo11 are influenced by both of local chromatin at hotspots and higher-order chromosome structures, formation of meiotic DSBs is under regulation of chromatin structure. Therefore, investigating features and roles of meiotic chromatin is crucial to elucidate the in vivo mechanism of meiotic recombination initiation. Recent progress in genome-wide chromatin analyses tremendously improved our understanding on this point, but many critical questions are left unaddressed. In this review, we summarize current knowledge in the field, and also discuss the future problems that must be solved to understand the role of chromatin structure in meiotic recombination.
authors.affiliation: Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Tokyo 153-8902, Japan. takayamada@bio.c.u-tokyo.ac.jp
authors.forename: Takatomi
authors.initials: T
authors.lastname: Yamada
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Ohta
mesh_terms: D000818:Animals; D002843:Chromatin / Q000235:genetics / Q000378:metabolism*; D053903:DNA Breaks, Double-Stranded; D004706:Endodeoxyribonucleases / Q000235:genetics / Q000378:metabolism; D055106:Genome-Wide Association Study; D006801:Humans; D008540:Meiosis / Q000502:physiology*; D011995:Recombination, Genetic / Q000502:physiology*; D000099257:Meiotic Recombination Protein SPO11
chemical_list: D002843:Chromatin; D004706:Endodeoxyribonucleases; D000099257:Meiotic Recombination Protein SPO11
keywords: DNA double-strand break; Spo11; chromatin; histone; meiotic recombination