title: Regulation of cell adhesion and migration by cell-derived matrices.
abstract: Three-dimensional in vitro extracellular matrix models provide a physiological alternative to regular two-dimensional cell culture, though they lack the full diversity of molecular composition and physical properties of whole-animal systems. Cell-derived matrices are extracellular matrices that are the product of matrix secretion and assembly by cells cultured at high density in vitro. After the removal of the cells that produced the matrix, an assembled matrix scaffold is left that closely mimics native stromal fiber organization and molecular content. Cell-derived matrices have been shown to impart in vivo-like responses to cells cultured in these matrices. In this review, we focus on mechanisms through which the distinct molecular and topographical composition of cell-derived matrices directs cellular behavior, specifically through regulation of cell-matrix adhesions and subsequent contributions to the process of cell migration.
authors.affiliation: Laboratory of Cell and Developmental Biology, National Institute of Dental and Craniofacial Research, United States. Electronic address: kutysml@mail.nih.gov.
authors.forename: Matthew L
authors.initials: ML
authors.lastname: Kutys
authors.forename: Andrew D
authors.initials: AD
authors.lastname: Doyle
authors.forename: Kenneth M
authors.initials: KM
authors.lastname: Yamada
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002465:Cell Movement / Q000502:physiology*; D021982:Cell-Matrix Junctions / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism*; D005347:Fibroblasts / Q000502:physiology; D006801:Humans
keywords: 3D cell migration; Cell-derived matrix (CDM); Extracellular matrix; Fibrillar topography; Matrix adhesions; Matrix elasticity