title: Use of expressed sequence tags as an alternative approach for the identification of Taenia solium metacestode excretion/secretion proteins.
abstract: BACKGROUND
  Taenia solium taeniasis/cysticercosis is a zoonotic helminth infection mainly found in rural regions of Africa, Asia and Latin America. In endemic areas, diagnosis of cysticercosis largely depends on serology, but these methods have their drawbacks and require improvement. This implies better knowledge of the proteins secreted and excreted by the parasite. In a previous study, we used a custom protein database containing protein sequences from related helminths to identify T. solium metacestode excretion/secretion proteins. An alternative or complementary approach would be to use expressed sequence tags combined with BLAST and protein mapping to supercontigs of Echinococcus granulosus, a closely related cestode. In this study, we evaluate this approach and compare the results to those obtained in the previous study.
  
  
  RESULTS
  We report 297 proteins organized in 106 protein groups based on homology. Additional classification was done using Gene Ontology information on biological process and molecular function. Of the 106 protein groups, 58 groups were newly identified, while 48 groups confirmed previous findings. Blast2GO analysis revealed that the majority of the proteins were involved in catalytic activities and binding.
  
  
  CONCLUSIONS
  In this study, we used translated expressed sequence tags combined with BLAST and mapping strategies to both confirm and complement previous research. Our findings are comparable to recent studies on other helminth genera like Echinococcus, Schistosoma and Clonorchis, indicating similarities between helminth excretion/secretion proteomes.
authors.affiliation: Veterinary Helminthology Unit, Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium. bvictor@itg.be
authors.forename: Bjorn
authors.initials: B
authors.lastname: Victor
authors.forename: Pierre
authors.initials: P
authors.lastname: Dorny
authors.forename: Kirezi
authors.initials: K
authors.lastname: Kanobana
authors.forename: Katja
authors.initials: K
authors.lastname: Polman
authors.forename: Johan
authors.initials: J
authors.lastname: Lindh
authors.forename: André M
authors.initials: AM
authors.lastname: Deelder
authors.forename: Magnus
authors.initials: M
authors.lastname: Palmblad
authors.forename: Sarah
authors.initials: S
authors.lastname: Gabriël
mesh_terms: D000818:Animals; D002589:Cestoda / Q000378:metabolism*; D020224:Expressed Sequence Tags; D015801:Helminth Proteins / Q000378:metabolism*; D041201:Taenia solium / Q000235:genetics / Q000502:physiology*
chemical_list: D015801:Helminth Proteins