title: De novo production of metabolites by fungal co-culture of Trichophyton rubrum and Bionectria ochroleuca.
abstract: The co-cultivation of fungi has recently been described as a promising strategy to induce the production of novel metabolites through possible gene activation. A large screening of fungal co-cultures in solid media has identified an unusual long-distance growth inhibition between Trichophyton rubrum and Bionectria ochroleuca. To study metabolite induction in this particular fungal interaction, differential LC-MS-based metabolomics was performed on pure strain cultures and on their co-cultures. The comparison of the resulting fingerprints highlighted five de novo induced compounds, which were purified using software-oriented semipreparative HPLC-MS. One metabolite was successfully identified as 4″-hydroxysulfoxy-2,2″-dimethylthielavin P (a substituted trimer of 3,5-dimethylorsellinic acid). The nonsulfated form, as well as three other related compounds, were found in the pure strain culture of B. ochroleuca.
authors.affiliation: School of Pharmaceutical Sciences, EPGL, University of Geneva, University of Lausanne, Quai Ernest-Ansermet 30, CH-1211 Geneva 4, Switzerland.
authors.forename: Samuel
authors.initials: S
authors.lastname: Bertrand
authors.forename: Olivier
authors.initials: O
authors.lastname: Schumpp
authors.forename: Nadine
authors.initials: N
authors.lastname: Bohni
authors.forename: Michel
authors.initials: M
authors.lastname: Monod
authors.forename: Katia
authors.initials: K
authors.lastname: Gindro
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Wolfender
mesh_terms: D002851:Chromatography, High Pressure Liquid; D018920:Coculture Techniques; D003470:Culture Media; D006999:Hypocreales / Q000737:chemistry / Q000235:genetics / Q000254:growth & development*; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D014249:Trichophyton / Q000737:chemistry / Q000235:genetics / Q000254:growth & development*
chemical_list: D003470:Culture Media