title: Cancer cell-specific photoactivity of pheophorbide a-glycol chitosan nanoparticles for photodynamic therapy in tumor-bearing mice.
abstract: We designed a cancer-cell specific photosensitizer nano-carrier by synthesizing pheophorbide a (PheoA) conjugated glycol chitosan (GC) with reducible disulfide bonds (PheoA-ss-GC). The amphiphilic PheoA-ss-GC conjugates self-assembled in aqueous condition to form core-shell structured nanoparticles (PheoA-ss-CNPs) with good colloidal stability and switchable photoactivity. The photoactivity of PheoA-ss-CNPs in an aqueous environment was greatly suppressed by the self-quenching effect, which enabled the PheoA-ss-CNPs to remain photo-inactive and in a quenched state. However, after the cancer cell-specific uptake, the nanoparticular structure instantaneously dissociated by reductive cleavage of the disulfide linkers, followed by an efficient dequenching process. Compared to non-reducible PheoA-conjugated GC-NPs with stable amide linkages (PheoA-CNPs), PheoA-ss-CNPs rapidly restored their photoactivity in response to intracellular reductive conditions, thus presenting higher cytotoxicity with light treatment. In addition, the PheoA-ss-CNPs presented prolonged blood circulation in vivo compared to free PheoA, demonstrating enhanced tumor specific targeting behavior through the enhanced permeation and retention (EPR) effect. The enhanced tumor accumulation of PheoA-ss-CNPs enabled tumor therapeutic efficacy that was more efficient than free PheoA in tumor-bearing mice. Based on the enhanced intracellular release for cytosolic high dose and switchable photoactivity mechanism for reduced side effects, these results suggest that PheoA-ss-CNPs have good potential for photodynamic therapy (PDT) in cancer treatment.
authors.affiliation: Department of Polymer Science & Engineering, Chungnam National University, 220, Gung-dong, Yuseng-gu, Daejeon 305-764, Republic of Korea.
authors.forename: In-hyeok
authors.initials: IH
authors.lastname: Oh
authors.forename: Hyun Su
authors.initials: HS
authors.lastname: Min
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Thanh Huyen
authors.initials: TH
authors.lastname: Tran
authors.forename: Yong-kyu
authors.initials: YK
authors.lastname: Lee
authors.forename: Ick Chan
authors.initials: IC
authors.lastname: Kwon
authors.forename: Kuiwon
authors.initials: K
authors.lastname: Choi
authors.forename: Kwangmeyung
authors.initials: K
authors.lastname: Kim
authors.forename: Kang Moo
authors.initials: KM
authors.lastname: Huh
mesh_terms: D000818:Animals; D048271:Chitosan / Q000737:chemistry / Q000493:pharmacokinetics / Q000627:therapeutic use*; D002734:Chlorophyll / Q000031:analogs & derivatives* / Q000737:chemistry / Q000493:pharmacokinetics / Q000627:therapeutic use; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D053758:Nanoparticles / Q000737:chemistry / Q000627:therapeutic use*; D009369:Neoplasms / Q000188:drug therapy* / Q000473:pathology; D010084:Oxidation-Reduction; D010778:Photochemotherapy; D011838:Radiation-Sensitizing Agents / Q000737:chemistry / Q000493:pharmacokinetics / Q000627:therapeutic use*
chemical_list: D011838:Radiation-Sensitizing Agents; C118638:glycol-chitosan; D002734:Chlorophyll; D048271:Chitosan; C032623:pheophorbide a