title: Antifungal activity of the lipopeptides produced by Bacillus amyloliquefaciens anti-CA against Candida albicans isolated from clinic.
abstract: The bacterium Bacillus amyloliquefaciens anti-CA isolated from mangrove system was found to be able to actively kill Candida albicans isolated from clinic. The bacterial strain anti-CA could produce high level of bioactive substance, amylase and protease in the cheap medium containing 2.0 % soybean meal, 2.0 % wheat flour, pH 6.5 within 26 h. After purification, the main bioactive substance was confirmed to be a cyclic lipopeptide containing a heptapeptide, L-Asp→L-Leu→L-Leu→L-Val→L-Val→L-Glu→L-Leu and a 3-OH fatty acid (15 carbons). In addition to C. albicans, the purified lipopeptide can also kill many yeast strains including Metschnikowia bicuspidata, Candida tropicalis, Yarrowia lipolytica and Saccharomyces cerevisiae. After treated by the purified lipopeptide, both the whole cells and protoplasts of C. albicans were destroyed.
authors.affiliation: Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao 266003, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Song
authors.forename: Yan-Jun
authors.initials: YJ
authors.lastname: Rong
authors.forename: Ming-Xin
authors.initials: MX
authors.lastname: Zhao
authors.forename: Zhen-Ming
authors.initials: ZM
authors.lastname: Chi
mesh_terms: D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001407:Bacillus / Q000737:chemistry*; D002176:Candida albicans / Q000187:drug effects* / Q000302:isolation & purification; D041022:Candida tropicalis / Q000187:drug effects; D002177:Candidiasis / Q000382:microbiology; D016000:Cluster Analysis; D003470:Culture Media / Q000737:chemistry / Q000191:economics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D055666:Lipopeptides / Q000302:isolation & purification / Q000494:pharmacology*; D055163:Metschnikowia / Q000187:drug effects; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000187:drug effects; D017422:Sequence Analysis, DNA; D025062:Yarrowia / Q000187:drug effects
chemical_list: D000935:Antifungal Agents; D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D055666:Lipopeptides; D012336:RNA, Ribosomal, 16S