title: Analysis of pyroptosis in bacterial infection.
abstract: Eukaryotic cells undergo death by several different mechanisms: apoptosis, a cell death that prevents inflammatory response; necrosis, when the cell membrane lyses and all the intracellular content is spilled outside; and pyroptosis, a cell death that is accompanied by the release of inflammatory cytokines by the dying cells. Pyroptosis is designed to attract a nonspecific innate response to the site of infection or tumor. In this chapter, we describe the methods used to study pyroptosis in a mammalian cell. The model organism used is Mycobacterium tuberculosis, which suppresses pyroptosis by macrophages, and possibly in dendritic cells.
authors.affiliation: Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR, USA.
authors.forename: Lia
authors.initials: L
authors.lastname: Danelishvili
authors.forename: Luiz E
authors.initials: LE
authors.lastname: Bermudez
mesh_terms: D017209:Apoptosis; D001424:Bacterial Infections / Q000473:pathology*; D020170:Caspase 1 / Q000378:metabolism; D002460:Cell Line; D016207:Cytokines / Q000378:metabolism*; D003584:Cytological Techniques / Q000379:methods*; D057075:Enzyme Assays; D006801:Humans; D020382:Interleukin-18 / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism; D008264:Macrophages / Q000382:microbiology; D009169:Mycobacterium tuberculosis
chemical_list: D016207:Cytokines; D020382:Interleukin-18; D053583:Interleukin-1beta; D020170:Caspase 1