title: Enrichment of cancer cells from whole blood using a microfabricated porous filter.
abstract: Enrichment of circulating tumor cells (CTCs) from whole blood is very challenging due to its rarity. We have developed a new CTC enrichment method using a microfabricated filter. The filter was designed to fractionate tumor cells by cell size and optimized to have high porosity and proper pore distribution. When cancer cells were spiked in whole blood, the average recovery rate was 82.0 to 86.7% and the limit of detection by filtration process was approximately 2 cancer cells in a testing volume of blood. The results indicate that the microfabricated filter-based enrichment would be useful to retrieve and analyze CTCs in practice.
authors.affiliation: CytoGen, Songpa-gu, Seoul 138-961, Republic of Korea.
authors.forename: Eun Hye
authors.initials: EH
authors.lastname: Kim
authors.forename: Jong Kil
authors.initials: JK
authors.lastname: Lee
authors.forename: Byung Chul
authors.initials: BC
authors.lastname: Kim
authors.forename: Sung Han
authors.initials: SH
authors.lastname: Rhim
authors.forename: Jhin Wook
authors.initials: JW
authors.lastname: Kim
authors.forename: Kyung Hee
authors.initials: KH
authors.lastname: Kim
authors.forename: Sung Mok
authors.initials: SM
authors.lastname: Jung
authors.forename: Pyeong Soo
authors.initials: PS
authors.lastname: Park
authors.forename: Hee Chul
authors.initials: HC
authors.lastname: Park
authors.forename: Jason
authors.initials: J
authors.lastname: Lee
authors.forename: Byung Hee
authors.initials: BH
authors.lastname: Jeon
mesh_terms: D045744:Cell Line, Tumor; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D048429:Cell Size; D005374:Filtration / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D055616:Microtechnology / Q000379:methods*; D009360:Neoplastic Cells, Circulating
keywords: Circulating tumor cells; Enrichment; Microfabricated filter