title: Engineered proteins with Pumilio/fem-3 mRNA binding factor scaffold to manipulate RNA metabolism.
abstract: Pumilio/fem-3 mRNA binding factor proteins are characterized by a sequence-specific RNA-binding domain. This unique single-stranded RNA recognition module, whose sequence specificity can be reprogrammed, has been fused with functional modules to engineer protein factors with various functions. We summarize the advances made with respect to developing RNA regulatory tools, as well as opportunities for the future.
authors.affiliation: Department of Pharmacology, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Zefeng
authors.initials: Z
authors.lastname: Wang
authors.forename: Traci M
authors.initials: TM
authors.lastname: Tanaka Hall
mesh_terms: D000818:Animals; D029742:Caenorhabditis elegans Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000627:therapeutic use; D029721:Drosophila Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000627:therapeutic use; D015195:Drug Design; D006801:Humans; D008958:Models, Molecular; D015202:Protein Engineering; D054730:Protein Interaction Domains and Motifs; D012313:RNA / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000627:therapeutic use; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism / Q000627:therapeutic use
chemical_list: D029742:Caenorhabditis elegans Proteins; D029721:Drosophila Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; D011993:Recombinant Fusion Proteins; C075059:fem-3 protein, C elegans; C074333:pum protein, Drosophila; D012313:RNA
keywords: PUF protein; RNA; RNA localization; RNA regulation; RNA-binding protein; X-ray crystallography; protein engineering; protein-RNA interaction; splicing; translation