title: The responses of Vitreoscilla hemoglobin-expressing hybrid aspen (Populus tremula × tremuloides) exposed to 24-h herbivory: expression of hemoglobin and stress-related genes in exposed and nonorthostichous leaves.
abstract: The responses of transcriptome and phenolic compounds were determined with Populus tremula L. × Populus tremuloides Michx. expressing the hemoglobin (Hb) of Vitreoscilla (VHb) and non-transformant (wt) line. After 24-h exposure of leaves to Conistra vaccinii L., the transcript levels of endogenous non-symbiotic class 1 Hb (PttHb1) and truncated Hb (PttTrHb) genes were modestly reduced and increased, respectively, in both wt and VHb-expressing line. Besides the herbivory exposed leaves showing the most significant transcriptome changes, alterations were also detected in the transcriptome of nonorthostichous leaves positioned directly above the exposed leaves. Both wt and VHb-expressing line displayed similar herbivory-induced effects on gene expression, although the extent of responses was more pronounced in the wt than in the VHb-expressing line. The contents of phenolic compounds were not altered due to herbivory and they were alike in the wt and VHb-expressing line. In addition, we determined the relative growth rates (RGRs) of Orthosia gothica L., Ectropis crepuscularia Denis & Schiff. and Orgyia antiqua L. larvae, and found no variation in the RGRs between the lines. Thus, VHb-expressing P. tremula × tremuloides lines showed to be comparable with wt in regards to the food quality of leaves.
authors.affiliation: Department of Biology, University of Oulu, P.O. Box 3000, 90014, Oulu, Finland, suvi.sutela@oulu.fi.
authors.forename: Suvi
authors.initials: S
authors.lastname: Sutela
authors.forename: Tiina
authors.initials: T
authors.lastname: Ylioja
authors.forename: Soile
authors.initials: S
authors.lastname: Jokipii-Lukkari
authors.forename: Anna-Kaisa
authors.initials: AK
authors.lastname: Anttila
authors.forename: Riitta
authors.initials: R
authors.lastname: Julkunen-Tiitto
authors.forename: Karoliina
authors.initials: K
authors.lastname: Niemi
authors.forename: Tiina
authors.initials: T
authors.lastname: Mölläri
authors.forename: Pauli T
authors.initials: PT
authors.lastname: Kallio
authors.forename: Hely
authors.initials: H
authors.lastname: Häggman
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D002678:Chimera; D015870:Gene Expression; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D060434:Herbivory; D062385:Hydroxybenzoates / Q000032:analysis; D007313:Insecta / Q000502:physiology; D007814:Larva; D020411:Oligonucleotide Array Sequence Analysis; D018515:Plant Leaves / Q000235:genetics / Q000469:parasitology / Q000502:physiology; D010940:Plant Proteins / Q000235:genetics; D030821:Plants, Genetically Modified; D032107:Populus / Q000235:genetics* / Q000502:physiology; D012333:RNA, Messenger / Q000235:genetics; D018749:RNA, Plant / Q000235:genetics; D013312:Stress, Physiological; D059467:Transcriptome; D054793:Truncated Hemoglobins / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D062385:Hydroxybenzoates; D010940:Plant Proteins; D012333:RNA, Messenger; D018749:RNA, Plant; D054793:Truncated Hemoglobins; C082613:hemoglobin protein, Vitreoscilla; C017616:phenolic acid