title: Remote postconditioning attenuates ischemia/reperfusion injury in rat skeletal muscle through mitochondrial ATP-sensitive K+ channel-dependent mechanism.
abstract: The authors investigated whether a remote postconditioning (remote post-con) procedure attenuated skeletal muscle ischemia/reperfusion (I/R) injury. We determined the optimal protocol of remote post-con and investigated its mechanism. Ischemia was induced for 3 hours in rat left hindlimb and three protocols of remote post-con were applied in right hindlimb just before the end of ischemia. The first (10-second group) involved two cycles of 10 seconds of occlusion followed by 10 seconds of reperfusion. The second (5-minute group) involved two cycles of 5 minutes of occlusion/reperfusion. The third (10-minute group) involved two cycles of 10 minutes of occlusion/reperfusion. In 5- and 10-minute groups, wet/dry ratio and muscle fiber edema were significantly lower than control group. Muscle contractility was preserved in 5- and 10-minute groups. An injection of 5-hydroxydecanoate (a specific blocker of mitochondrial ATP-sensitive K+ [mKATP] channels) impaired this effect. This study demonstrates that remote post-con preserves muscle contractility and reduces tissue edema and necrosis, possibly through the activation of mKATP channels. We suggest that two cycles of 5 minutes of occlusion followed by 5 minutes of reperfusion are optimal protocols of remote post-con in skeletal muscle I/R injury.
authors.affiliation: Department of Orthopedic Surgery, Inje University Ilsan Paik Hospital, Goyang, Korea.
authors.forename: Jung Il
authors.initials: JI
authors.lastname: Lee
authors.forename: Kyung
authors.initials: K
authors.lastname: Wook Nha
authors.forename: Jin Soo
authors.initials: JS
authors.lastname: Suh
authors.forename: Suk Kyu
authors.initials: SK
authors.lastname: Choo
authors.forename: Jung Ho
authors.initials: JH
authors.lastname: Park
authors.forename: Jong Woong
authors.initials: JW
authors.lastname: Park
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D004487:Edema / Q000517:prevention & control; D057775:Ischemic Postconditioning / Q000379:methods*; D008297:Male; D008928:Mitochondria / Q000378:metabolism; D009119:Muscle Contraction / Q000502:physiology; D018482:Muscle, Skeletal / Q000098:blood supply*; D015221:Potassium Channels / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000503:physiopathology / Q000517:prevention & control*
chemical_list: D015221:Potassium Channels; D000255:Adenosine Triphosphate