title: Optimization of extraction of antioxidants from wheat bran (Triticum spp.) using response surface methodology.
abstract: In this study, the optimal conditions for the extraction of antioxidants from hard winter wheat bran (WH711) were determined using response surface methodology (RSM). A central composite face centred design was used to investigate the effects of three independent variables, namely solvent composition (%v/v), extraction temperature (°C) and time (minutes) on the responses; free phenolic content (FPC), total flavonoid content (TFC), total antioxidant activity (TAA), ferric reducing power (FRP), hydrogen peroxide scavenging activity (HPSA). Regression analysis showed that more than 90% of the variation was explained by the models of different responses. The optimal conditions for the antioxidantss extraction were found to be methanol concentration of 85%v/v, extraction temperature of 75 °C, extraction time of 45 min, for wheat bran. The experimental values of FPC, TFC, TAA, FRP and HPSA were 0.921 mg GAE/g bran (B), 0.4588 mg CE/g B, 0.01408 mM AAE/g B, 2.532 mM AAE/g B and 3.193 mM TE/g B, respectively which agreed with those predicted, thus indicating suitability of the model employed and the suitability of RSM in optimizing the extraction conditions.
authors.affiliation: Department of Food Engineering and Technology, Sant Longowal Institute of Engineering and Technology (Deemed To Be University), Longowal, Dist.-Sangrur, Punjab India 148106.
authors.forename: Barinderjit
authors.initials: B
authors.lastname: Singh
authors.forename: Harish Kumar
authors.initials: HK
authors.lastname: Sharma
authors.forename: Bhavesh Chandra
authors.initials: BC
authors.lastname: Sarkar
keywords: Extraction; Free phenolic compound (FPC); Response surface methodology (RSM); Total antioxidant activity (TAA); Total flavonoid compound (TFC); Wheat bran