title: Insulin-like growth factor 1 is not associated with high myopia in a large Japanese cohort.
abstract: OBJECTIVE
  To investigate whether genetic variations in the insulin-like growth factor 1 (IGF-1) gene are associated with high myopia in Japanese.
  
  
  METHODS
  A total of 1,339 unrelated Japanese patients with high myopia (axial length ≥26 mm in both eyes) and two independent control groups were evaluated (334 cataract patients without high myopia and 1,194 healthy Japanese individuals). The mean axial length (mm±SD) in the case group was 29.18±1.85 mm, and the mean spherical equivalent (D±SD) of the phakic eyes was -12.69±4.54 D. We genotyped five tagging single nucleotide polymorphisms (SNPs) in IGF-1: rs6214, rs978458, rs5742632, rs12423791, and rs2162679. Chi-square tests for trend, multivariable logistic regression, and haplotype regression analysis were conducted.
  
  
  RESULTS
  We found no significant association between the IGF-1 SNPs and high or extreme myopia (axial length ≥28 mm in both eyes, 837 subjects) in the additive model, even when compared with the cataract and general population controls, with or without adjustments for age and sex. The evaluation using dominant and recessive models also did not reveal any significant associations. The haplotype analysis with a variable-sized sliding-window strategy also showed a lack of association of IGF-1 SNPs with high or extreme myopia.
  
  
  CONCLUSIONS
  The results of the present study using a Japanese subset do not support the proposal that the IGF-1 gene determines susceptibility to high or extreme myopia in Caucasians and Chinese. Further studies are needed to confirm our reports in other populations and to identify the underlying genetic determinants of these ocular pathological conditions.
authors.affiliation: Department of Ophthalmology and Visual Sciences, Tokyo, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Miyake
authors.forename: Kenji
authors.initials: K
authors.lastname: Yamashiro
authors.forename: Hideo
authors.initials: H
authors.lastname: Nakanishi
authors.forename: Isao
authors.initials: I
authors.lastname: Nakata
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Akagi-Kurashige
authors.forename: Akitaka
authors.initials: A
authors.lastname: Tsujikawa
authors.forename: Muka
authors.initials: M
authors.lastname: Moriyama
authors.forename: Kyoko
authors.initials: K
authors.lastname: Ohno-Matsui
authors.forename: Manabu
authors.initials: M
authors.lastname: Mochizuki
authors.forename: Ryo
authors.initials: R
authors.lastname: Yamada
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Matsuda
authors.forename: Nagahisa
authors.initials: N
authors.lastname: Yoshimura
mesh_terms: D000368:Aged; D044466:Asian People / Q000235:genetics*; D016022:Case-Control Studies; D015331:Cohort Studies; D003710:Demography; D005260:Female; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000235:genetics*; D007564:Japan; D008297:Male; D008875:Middle Aged; D009216:Myopia / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: C583557:IGF1 protein, human; D007334:Insulin-Like Growth Factor I