title: In vitro degradation of oxalate by recombinant Lactobacillus plantarum expressing heterologous oxalate decarboxylase.
abstract: OBJECTIVE
  The aim of the present study is to constitutively express heterologous oxalate decarboxylase (OxdC) in Lactobacillus plantarum and to examine its ability to degrade oxalate in vitro for their future therapy against enteric hyperoxaluria.
  
  
  RESULTS
  In this study, we generated a recombinant strain of Lb. plantarum to constitutively overexpress B. subtilis oxalate decarboxylase (oxdC) using a host lactate dehydrogenase promoter (PldhL ). The recombinant Lb. plantarum was able to degrade more than 90% oxalate compared to 15% by the wild type. In addition, the recombinant strain also had higher tolerance up to 500 mmol l(-1) oxalate.
  
  
  CONCLUSIONS
  We developed a recombinant Lb. plantarum NC8 that constitutively expressed heterologous oxalate decarboxylase and degraded oxalate efficiently under in vitro conditions.
  
  
  CONCLUSIONS
  The long-term aim is to develop an efficient strain for future therapy against oxalosis.
authors.affiliation: Department of Biochemistry, Centre for Excellence in Genomic Sciences, School of Biological Sciences, Madurai Kamaraj University, Madurai, India.
authors.forename: K
authors.initials: K
authors.lastname: Anbazhagan
authors.forename: P
authors.initials: P
authors.lastname: Sasikumar
authors.forename: S
authors.initials: S
authors.lastname: Gomathi
authors.forename: H P
authors.initials: HP
authors.lastname: Priya
authors.forename: G S
authors.initials: GS
authors.lastname: Selvam
mesh_terms: D002262:Carboxy-Lyases / Q000235:genetics / Q000378:metabolism*; D048191:Lactiplantibacillus plantarum / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D010070:Oxalates / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D010070:Oxalates; D011994:Recombinant Proteins; D002262:Carboxy-Lyases; C021915:oxalate decarboxylase
keywords: hyperoxaluria; oxalate degradation; probiotic potential; recombinant Lactobacillus plantarum