title: Oxidative stress status in umbilical cord blood from neonates born to mothers with atopic asthma.
abstract: OBJECTIVE
  Our objective was to assess the oxidative stress status and analyse the relationship between an oxidant/antioxidant imbalance and the mediator release properties of cord blood basophils from neonates born to mothers with atopic asthma.
  
  
  METHODS
  Cord blood was collected from the neonates of 16 asthmatic mothers and 18 healthy mothers Basophils were purified and stimulated by Dermatophagoides farinae (Df), hyperosmotic mannitol or peptidoglycan (PGN). Immunoblotting detected nuclear factor κB (NFκB) as a measure of functional receptor response. The linear correlations between IL-4 levels in the supernatants and 3-nitrotyrosine (3-NT), glutathione peroxidase (GSH-Px) in the serum were evaluated.
  
  
  RESULTS
  Compared with the healthy group, the levels of 3-NT in maternal blood and cord blood were significantly higher in allergic asthma group, whereas the GSH-Px activity were lower. After stimulation, cord blood basophils from the neonates born to atopic mothers produced more IL-4 involving NF-κB pathways. There was a significant relationship between the IL-4 levels produced by basophils and 3-NT (or GSH-Px) in cord blood from allergic asthma group.
  
  
  CONCLUSIONS
  In asthma, mediator release properties of human basophils induced by environmental allergens and irritants are associated with oxidative stress, which may be one of the pathogenesis of allergic diseases.
authors.affiliation: Department of Geriatrics, Xinhua Hospital .
authors.forename: Yang
authors.initials: Y
authors.lastname: Ling
authors.forename: Guo
authors.initials: G
authors.lastname: Yin-Shi
authors.forename: Shan
authors.initials: S
authors.lastname: Hong-Wei
authors.forename: Zhou
authors.initials: Z
authors.lastname: Yan
authors.forename: Li
authors.initials: L
authors.lastname: Qiang
authors.forename: Tian
authors.initials: T
authors.lastname: Ye
authors.forename: Xiong
authors.initials: X
authors.lastname: Ying
mesh_terms: D000328:Adult; D001249:Asthma / Q000097:blood / Q000150:complications* / Q000276:immunology; D001491:Basophils / Q000378:metabolism; D005260:Female; D005312:Fetal Blood / Q000166:cytology*; D005979:Glutathione Peroxidase / Q000097:blood; D006801:Humans; D006969:Hypersensitivity, Immediate / Q000097:blood / Q000150:complications* / Q000276:immunology; D007231:Infant, Newborn; D015847:Interleukin-4 / Q000097:blood; D018384:Oxidative Stress; D011247:Pregnancy; D011248:Pregnancy Complications / Q000276:immunology*; D014443:Tyrosine / Q000031:analogs & derivatives / Q000097:blood
chemical_list: D015847:Interleukin-4; C002744:3-nitrotyrosine; D014443:Tyrosine; D005979:Glutathione Peroxidase