title: Preparation and characterization of glucose oxidase nanoparticles and their application in dissolved oxygen metric determination of serum glucose.
abstract: The nanoparticle (NP) aggregates of commercial glucose oxidase (GOD) from A. niger with 117 nm diameter, were prepared by desolvation method. The formation and characterization of GOD-NP aggregates was studied by UV, transmission electron microscopic (TEM) and Fourier transform infrared (FTIR) spectra. GOD-NP aggregates were more stable, active and had a higher shelf life than that of free enzyme. These GOD-NP aggregates were immobilized onto chitosan activated nitrocellulose (NC) membrane through glutaraldehyde coupling with 55.15% retention of initial activity of free enzyme and 5.9 microg protein/cm2 yield. The membrane bound GOD-NP aggregates showed optimum response within 60 s, at pH 6.0, temperature range 30-45 degrees C with a peak at 35 degrees C and a hyperbolic relationship with glucose upto 250 mg/dl. Apparent K(m) and V(max) were 75 mg/dl and 0.06 mg/l respectively. The NC membrane bound GOD-NP aggregates were employed for dissolved O2 (DO) metric determination of serum glucose in apparently healthy and diabetic adults. The present method of serum glucose determination was evaluated.
authors.affiliation: Department of Biochemistry, Maharshi Dayanand University, Rohtak 124001, Haryana, India.
authors.forename: Nidhi
authors.initials: N
authors.lastname: Kundu
authors.forename: Sandeep
authors.initials: S
authors.lastname: Yadav
authors.forename: C S
authors.initials: CS
authors.lastname: Pundir
mesh_terms: D015374:Biosensing Techniques; D001786:Blood Glucose / Q000032:analysis*; D004566:Electrodes; D005949:Glucose Oxidase / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D001786:Blood Glucose; D005949:Glucose Oxidase