title: Facile characterization of the immobilization of streptavidin on magnetic submicron particles with a fluorescent probe of streptavidin.
abstract: To characterize streptavidin immobilization on magnetic submicron particles (MSPs), residual streptavidin after magnetic removal of immobilized streptavidin was quantified with N-biotinyl-N'-(1-naphthyl)-ethylenediamine (BNEDA) based on Förster resonance energy transfer. Residual BNEDA after magnetic removal of bound BNEDA was measured by its own fluorescence. Streptavidin was immobilized at about 12 mg per gram of MSPs and easily retained over 50% of its original activity. These assays facilitated optimized streptavidin immobilization on MSPs.
authors.affiliation: Unit for Analytical Probes and Protein Biotechnology, Key Laboratory of Clinical Laboratory Diagnostics of the Education Ministry, College of Laboratory Medicine, Chongqing Medical University, Chongiqng 400016, China.
authors.forename: Gaobo
authors.initials: G
authors.lastname: Long
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Yang
authors.forename: Jun
authors.initials: J
authors.lastname: Pu
authors.forename: Jialin
authors.initials: J
authors.lastname: Qin
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
authors.forename: Fei
authors.initials: F
authors.lastname: Liao
mesh_terms: D001710:Biotin / Q000031:analogs & derivatives / Q000032:analysis / Q000737:chemistry; D005029:Ethylenediamines / Q000032:analysis / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry*; D055767:Immobilized Proteins / Q000737:chemistry* / Q000378:metabolism; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry* / Q000378:metabolism
chemical_list: D005029:Ethylenediamines; D005456:Fluorescent Dyes; D055767:Immobilized Proteins; D058185:Magnetite Nanoparticles; C093128:N-biotinoyl-N'-iodoacetylethylenediamine; D001710:Biotin; D019809:Streptavidin