title: Distribution of Two bioactive compounds in flowers of Trollius chinensis.
abstract: A comprehensive procedure was established, which combined a high-performance liquid chromatography (HPLC) assay for the simultaneous quantification of 2″-O-β-L-galactopyranosylorientin (OGA) and veratric acid and a gravimetric analysis for the determination of the mass fraction of the floral parts (calyx, corolla, stalk, stamens and pistils and ovary) of Trollius chinensis, to investigate the distribution and identify the enriched floral part(s) of these two compounds in the flowers. The calculated mean distributions of OGA in calyx, corolla, stamens and pistils, stalk and ovary were 83.62, 7.76, 4.35, 2.92 and 1.35%, respectively, whereas those of veratric acid in the corresponding floral parts were 46.41, 9.01, 18.41, 4.11 and 22.06%, respectively, indicating the uneven and noncorresponding distribution of these two compounds. This study extends the application of the HPLC assay and favors the production of OGA and veratric acid from the flowers of T. chinensis in addition to the benefits of breeding, cultivation and utilization of these flowers.
authors.affiliation: 1School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Yuan
authors.forename: Yan-Nan
authors.initials: YN
authors.lastname: An
authors.forename: Ru-Feng
authors.initials: RF
authors.lastname: Wang
authors.forename: Yi
authors.initials: Y
authors.lastname: Ding
authors.forename: Zhen-Xiao
authors.initials: ZX
authors.lastname: Sun
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004187:Disaccharides / Q000302:isolation & purification* / Q000378:metabolism; D005419:Flavonoids / Q000302:isolation & purification* / Q000378:metabolism; D035264:Flowers / Q000737:chemistry* / Q000378:metabolism; D009928:Organ Specificity; D010936:Plant Extracts / Q000302:isolation & purification*; D029626:Ranunculaceae / Q000737:chemistry* / Q000378:metabolism; D015203:Reproducibility of Results; D013046:Specific Gravity; D014641:Vanillic Acid / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism
chemical_list: C000597391:2''-O-galactopyranosylorientin; D004187:Disaccharides; D005419:Flavonoids; D010936:Plant Extracts; C009333:veratric acid; D014641:Vanillic Acid