title: Label-free and reagentless electrochemical detection of microRNAs using a conducting polymer nanostructured by carbon nanotubes: application to prostate cancer biomarker miR-141.
abstract: In this paper, a label-free and reagentless microRNA sensor based on an interpenetrated network of carbon nanotubes and electroactive polymer is described. The nanostructured polymer film presents very well-defined electroactivity in neutral aqueous medium in the cathodic potential domain from the quinone group embedded in the polymer backbone. Addition of microRNA miR-141 target (prostate cancer biomarker) gives a "signal-on" response, i.e. a current increase due to enhancement of the polymer electroactivity. On the contrary, non-complementary miRNAs such as miR-103 and miR-29b-1 do not lead to any significant current change. A very low detection limit of ca. 8 fM is achieved with this sensor.
authors.affiliation: Université Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205 Paris Cedex 13, France.
authors.forename: H V
authors.initials: HV
authors.lastname: Tran
authors.forename: B
authors.initials: B
authors.lastname: Piro
authors.forename: S
authors.initials: S
authors.lastname: Reisberg
authors.forename: L D
authors.initials: LD
authors.lastname: Tran
authors.forename: H T
authors.initials: HT
authors.lastname: Duc
authors.forename: M C
authors.initials: MC
authors.lastname: Pham
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004553:Electric Conductivity; D055664:Electrochemical Techniques / Q000379:methods*; D006801:Humans; D057230:Limit of Detection; D008297:Male; D035683:MicroRNAs / Q000032:analysis*; D008958:Models, Molecular; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure; D011108:Polymers / Q000737:chemistry*; D011471:Prostatic Neoplasms / Q000175:diagnosis*
chemical_list: C533895:MIRN141 microRNA, human; D035683:MicroRNAs; D037742:Nanotubes, Carbon; D011108:Polymers
keywords: Conducting polymer; Electrochemical biosensor; Label-free detection; MicroRNA; Oligonucleotides; Square wave voltammetry