title: Detection of previously unknown fumonisin P analogue mycotoxins in a Fusarium verticillioides culture by high-performance liquid chromatography-electrospray ionization time-of-flight and ion trap mass spectrometry.
abstract: Five previously unknown fumonisin mycotoxins (iso-FP1, iso-FP(2,3a), iso-FP(2,3b), FP4 and iso-FP4) and three previously described FP analogues (FP(1-3)) were detected in a solid rice culture inoculated with Fusarium verticillioides. The fumonisins were characterized by high-performance liquid chromatography-electrospray ionization time-of-flight mass spectrometry (HPLC-ESI-TOFMS) and ion trap mass spectrometry (ITMS). The FP isomers were separated by using a flat gradient on a special, high-coverage C18, narrow-bore HPLC column (YMC-Pack J'sphere ODS H80), which was suggested for the separation of structural isomers. The verified structures of the FP(1-3) mycotoxins, the relative retention times (by HPLC-ESI-TOFMS and ITMS), the exact masses of the molecular ions (by TOFMS) and the masses of the product ions, including the hydrocarbon backbones (by ITMS) of the new compounds, strongly suggested their structures.
authors.affiliation: Fumizol Ltd., Moszkvai krt. 5-7, H-6725 Szeged, Hungary tibor.bartok@fumizol.hu.
authors.forename: Tibor
authors.initials: T
authors.lastname: Bartók
authors.affiliation: Eötvös Loránd University Joint Research and Training Laboratory on Separation Techniques, Pázmány Péter sétány 1/A, H-1117 Budapest, Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Tölgyesi
authors.affiliation: Research Centre for Agriculture, Hungarian Academy of Sciences, Research Institute for Plant Protection, Herman Ottó út 15, H-1022 Budapest, Hungary.
authors.forename: Árpád
authors.initials: Á
authors.lastname: Szécsi
authors.affiliation: Cereal Research Non-Profit Ltd., Alsókikötő sor 9, H-6726 Szeged, Hungary.
authors.forename: Ákos
authors.initials: Á
authors.lastname: Mesterházy
authors.affiliation: Stereochemistry Research Group of the Hungarian Academy of Sciences, Dóm tér 8, H-6720 Szeged, Hungary.
authors.forename: Mihály
authors.initials: M
authors.lastname: Bartók
authors.affiliation: Department of Food Engineering, Faculty of Engineering, University of Szeged, Moszkvai krt. 5-7, H-6725 Szeged, Hungary.
authors.forename: Ernő
authors.initials: E
authors.lastname: Gyimes
authors.affiliation: Department of Food Engineering, Faculty of Engineering, University of Szeged, Moszkvai krt. 5-7, H-6725 Szeged, Hungary.
authors.forename: Antal
authors.initials: A
authors.lastname: Véha
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D037341:Fumonisins / Q000032:analysis*; D005670:Fusarium / Q000737:chemistry*; D009183:Mycotoxins / Q000032:analysis*; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D037341:Fumonisins; D009183:Mycotoxins