title: Quantification of major royal jelly protein 1 in fresh royal jelly by indirect enzyme-linked immunosorbent assay.
abstract: Rabbit polyclonal antibody produced by a major royal jelly protein 1 (MRJP1) specific peptide reacted only with a MRJP1. Indirect ELISA with the antibody revealed a MRJP1 level of 4.12-4.67 g/100 g in different company's royal jelly, which almost agreed with that of a hexametric form of MRJP1 (apisin) measured by high performance liquid chromatography. These results suggest that MRJP1 exists mainly as apisin in royal jelly.
authors.affiliation: College of Plant Protection, Yunnan Agricultural University, Yunnan, China.
authors.forename: Kikuji
authors.initials: K
authors.lastname: Yamaguchi
authors.forename: Shaoyu
authors.initials: S
authors.lastname: He
authors.forename: Zhengyue
authors.initials: Z
authors.lastname: Li
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Murata
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Hitomi
authors.forename: Manaho
authors.initials: M
authors.lastname: Mozumi
authors.forename: Risa
authors.initials: R
authors.lastname: Ariga
authors.forename: Toshiki
authors.initials: T
authors.lastname: Enomoto
mesh_terms: D000818:Animals; D017306:Annexin A2 / Q000737:chemistry; D000906:Antibodies / Q000276:immunology*; D002851:Chromatography, High Pressure Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D005227:Fatty Acids / Q000737:chemistry* / Q000378:metabolism; D006023:Glycoproteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D019476:Insect Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D000099219:Royal Jelly
chemical_list: D017306:Annexin A2; D000906:Antibodies; D005227:Fatty Acids; D006023:Glycoproteins; D019476:Insect Proteins; D000099219:Royal Jelly; C559157:MRJP1 protein, Apis mellifera