title: Blastocyst biopsy with comprehensive chromosome screening and fresh embryo transfer significantly increases in vitro fertilization implantation and delivery rates: a randomized controlled trial.
abstract: OBJECTIVE
  To determine whether blastocyst biopsy and rapid quantitative real-time polymerase chain reaction (qPCR)-based comprehensive chromosome screening (CCS) improves in vitro fertilization (IVF) implantation and delivery rates.
  
  
  METHODS
  Randomized controlled trial.
  
  
  METHODS
  Academic reproductive medicine center.
  
  
  METHODS
  Infertile couples in whom the female partner (or oocyte donor) is between the ages of 21 and 42 years who are attempting conception through IVF.
  
  
  METHODS
  Embryonic aneuploidy screening.
  
  
  METHODS
  Sustained implantation and delivery rates.
  
  
  RESULTS
  We transferred 134 blastocysts to 72 patients in the study (CCS) group and 163 blastocysts to 83 patients in the routine care (control) group. Sustained implantation rates (probability that an embryo will implant and progress to delivery) were statistically significantly higher in the CCS group (89 of 134; 66.4%) compared with those from the control group (78 of 163; 47.9%). Delivery rates per cycle were also statistically significantly higher in the CCS group. Sixty one of 72 treatment cycles using CCS led to delivery (84.7%), and 56 of 83 (67.5%) control cycles ultimately delivered. Outcomes were excellent in both groups, but use of CCS clearly improved patient outcomes.
  
  
  CONCLUSIONS
  Blastocyst biopsy with rapid qPCR-based comprehensive chromosomal screening results in statistically significantly improved IVF outcomes, as evidenced by meaningful increases in sustained implantation and delivery rates.
  
  
  BACKGROUND
  NCT01219283.
authors.affiliation: Reproductive Medicine Associates of New Jersey, Morristown, New Jersey, USA. rscott@rmanj.com
authors.forename: Richard T
authors.initials: RT
authors.lastname: Scott
authors.forename: Kathleen M
authors.initials: KM
authors.lastname: Upham
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Forman
authors.forename: Kathleen H
authors.initials: KH
authors.lastname: Hong
authors.forename: Katherine L
authors.initials: KL
authors.lastname: Scott
authors.forename: Deanne
authors.initials: D
authors.lastname: Taylor
authors.forename: Xin
authors.initials: X
authors.lastname: Tao
authors.forename: Nathan R
authors.initials: NR
authors.lastname: Treff
mesh_terms: D000328:Adult; D001706:Biopsy / Q000379:methods; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism / Q000473:pathology*; D002869:Chromosome Aberrations / Q000196:embryology; D020732:Cytogenetic Analysis / Q000379:methods / Q000706:statistics & numerical data; D036861:Delivery, Obstetric / Q000706:statistics & numerical data*; D010064:Embryo Implantation / Q000235:genetics; D004624:Embryo Transfer / Q000706:statistics & numerical data; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods; D006801:Humans; D007231:Infant, Newborn; D007246:Infertility / Q000453:epidemiology / Q000473:pathology / Q000628:therapy; D008297:Male; D011247:Pregnancy; D018873:Pregnancy Rate; D019836:Preimplantation Diagnosis / Q000379:methods*; D055815:Young Adult
keywords: IVF; blastocyst; comprehensive chromosomal screening; embryonic aneuploidy; preimplantation genetic screening