title: Chemical method for retrieval of cells encapsulated in alginate-polyethersulfone microcapsules.
abstract: We present a method for retrieval of cells encapsulated in alginate-polyethersulfone (AP) microcapsules. AP microcapsules consist of alginate hydrogel core and a semi-permeable polymer membrane. Proposed method is based on chemical destruction of microcapsule structure that is, polymer membranes are removed by dissolution in 1-methyl-2-pyrrolidone subsequently the cells immobilized in the cores are released by liquefaction of alginate gel. The viability of Saccharomyces cerevisiae cells retrieved by this technique exceeds 98%, while the concentration of recovered cells reaches 98%-102% of cell concentration in suspension used for microencapsulation, which proves that proposed method allows to effectively and quantitatively retrieve encapsulated cells without compromising their viability.
authors.affiliation: Department of Biomedical Systems and Technologies, Laboratory of Microencapsulations, Nałęcz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences , Warsaw , Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Kupikowska-Stobba
authors.forename: Dorota
authors.initials: D
authors.lastname: Lewińska
authors.forename: Marcin
authors.initials: M
authors.lastname: Grzeczkowicz
mesh_terms: D000464:Alginates / Q000737:chemistry*; D002214:Capsules; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000166:cytology*; D013450:Sulfones / Q000737:chemistry*; D013997:Time Factors
chemical_list: D000464:Alginates; D002214:Capsules; D006603:Hexuronic Acids; D011108:Polymers; D013450:Sulfones; C022840:polyether sulfone; D020723:Glucuronic Acid
keywords: alginate-polyethersulfone microcapsules; cell concentration; cell microencapsulation; cell recovery; cell retrieval; core-shell microcapsules; polymer microcapsules