title: DJ-1 cooperates with PYCR1 in cell protection against oxidative stress.
abstract: DJ-1, a product of the DJ-1/PARK7 gene, has been suggested to play various functions involved in transcriptional regulation, protease activity, anti-oxidative stress activity, and regulation of mitochondrial complex I. Such a variety of functions of DJ-1 are supposed to be realized through interactions with different partner proteins. Among the candidates for DJ-1-partner proteins detected in TOF-MAS analyses of the cellular proteins co-immunoprecipitated with DJ-1, we focused here pyrroline-5-carboxylate reductase 1, PYCR1, a final key enzyme for proline biosynthesis. DJ-1 directly bound to PYCR1 in vivo and in vitro. DJ-1 and PYCR1 colocalized in mitochondria, and both were suggested to be involved in regulation of mitochondrial membrane potential, but differently. DJ-1 enhanced the enzymatic activity of PYCR1 in vitro. The cells knocked down for DJ-1 and PYCR1 showed lower viability under oxidative stress conditions. No additive nor synergistic results were obtained for the cells that had been knocked down for both DJ-1 and PYCR1, suggesting that DJ-1 and PYCR1 are on the same pathway of anti-oxidative stress protection of the cells.
authors.affiliation: Graduate School of Life Science, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Tatsuki
authors.initials: T
authors.lastname: Yasuda
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Kaji
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Agatsuma
authors.forename: Takeshi
authors.initials: T
authors.lastname: Niki
authors.forename: Mitsuhiro
authors.initials: M
authors.lastname: Arisawa
authors.forename: Satoshi
authors.initials: S
authors.lastname: Shuto
authors.forename: Hiroyoshi
authors.initials: H
authors.lastname: Ariga
authors.forename: Sanae M M
authors.initials: SM
authors.lastname: Iguchi-Ariga
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D019084:Fluorescent Antibody Technique, Indirect; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D008928:Mitochondria / Q000378:metabolism; D015513:Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress; D054464:Peroxiredoxins; D011394:Proline Oxidase / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding / Q000187:drug effects; D000071617:Protein Deglycase DJ-1; D011762:Pyrroline Carboxylate Reductases / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D015398:Signal Transduction / Q000187:drug effects; D000097502:delta-1-Pyrroline-5-Carboxylate Reductase
chemical_list: D006861:Hydrogen Peroxide; D047908:Intracellular Signaling Peptides and Proteins; D015513:Oncogene Proteins; D016877:Oxidants; D054464:Peroxiredoxins; D011394:Proline Oxidase; D000071617:Protein Deglycase DJ-1; D011762:Pyrroline Carboxylate Reductases; D000097502:delta-1-Pyrroline-5-Carboxylate Reductase; C105131:PARK7 protein, human; C503466:PARK7 protein, mouse