title: Very low levels of surface CD45 reflect CLL cell fragility, are inversely correlated with trisomy 12 and are associated with increased treatment-free survival.
abstract: It has recently been suggested that the percentage of smudge cells on blood smears from patients with chronic lymphocytic leukemia (CLL) could predict overall survival. However, smudge cells are a cytological artifact influenced by multiple physical factors not related to CLL. To identify simple parameters reflecting CLL cell fragility, we studied CD45 expression in a series of 66 patients with Binet stage A CLL. Decreased CD45 expression was specific for CLL cells when compared to 44 patients with a leukemic phase of B-cell non Hodgkin lymphoma and 42 control B-cells. CD45 expression was markedly decreased for all patients with CLL with high percentages of smudge cells. CLL cells with the lowest CD45 expression were the most sensitive to osmotic shock. Very low levels of CD45 expression were significantly associated with lack of CD38 expression, absence of trisomy 12, and with increased treatment free survival time. Altogether, these results demonstrate that low levels of CD45 expression are specific to CLL cells and reflect cell fragility, suggesting that this is an important intrinsic biological feature that determines disease course.
authors.affiliation: Laboratory of Hematology, University Hospital Dupuytren, Limoges, France.
authors.forename: David
authors.initials: D
authors.lastname: Rizzo
authors.forename: Angad
authors.initials: A
authors.lastname: Lotay
authors.forename: Nathalie
authors.initials: N
authors.lastname: Gachard
authors.forename: Ibtissam
authors.initials: I
authors.lastname: Marfak
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Faucher
authors.forename: Franck
authors.initials: F
authors.lastname: Trimoreau
authors.forename: Estelle
authors.initials: E
authors.lastname: Guérin
authors.forename: Dominique
authors.initials: D
authors.lastname: Bordessoule
authors.forename: Arnaud
authors.initials: A
authors.lastname: Jaccard
authors.forename: Jean
authors.initials: J
authors.lastname: Feuillard
mesh_terms: D051997:ADP-ribosyl Cyclase 1 / Q000235:genetics; D001402:B-Lymphocytes / Q000378:metabolism* / Q000473:pathology; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D018450:Disease Progression; D018572:Disease-Free Survival; D015536:Down-Regulation; D015870:Gene Expression; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D017493:Leukocyte Common Antigens / Q000235:genetics*; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D008562:Membrane Glycoproteins / Q000235:genetics; D009367:Neoplasm Staging; D009997:Osmotic Pressure; D014314:Trisomy
chemical_list: D015415:Biomarkers; D008562:Membrane Glycoproteins; D017493:Leukocyte Common Antigens; C516769:PTPRC protein, human; C498154:CD38 protein, human; D051997:ADP-ribosyl Cyclase 1