title: A universal oligonucleotide microarray with a minimal number of probes for the detection and identification of viroids at the genus level.
abstract: A major challenge in the agricultural industry is the development of techniques that can screen plant samples for viroid infection. Microarrays are promising in this regard, as their high throughput nature can potentially allow for the detection of a range of viroids in a single test. In this paper we present a microarray that can detect a wide spectrum of all 8 reported viroid genera including 37 known plant viroid species. The array was constructed using an automated probe design protocol which generated a minimal number of probes to detect viroids at the genus level. The designed microarray showed a high specificity and sensitivity when tested with a set of standard virus samples. Finally, the microarray was applied to screen infected field samples, with Hop stunt viroid infection identified as the major disease causing pathogen for an infected citrus sample.
authors.affiliation: Chinese Academy of Inspection and Quarantine, Beijing, China.
authors.forename: Yongjiang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Yin
authors.forename: Dongmei
authors.initials: D
authors.lastname: Jiang
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Xin
authors.forename: Fang
authors.initials: F
authors.lastname: Ding
authors.forename: Ziniu
authors.initials: Z
authors.lastname: Deng
authors.forename: Guoping
authors.initials: G
authors.lastname: Wang
authors.forename: Xianfeng
authors.initials: X
authors.lastname: Ma
authors.forename: Fang
authors.initials: F
authors.lastname: Li
authors.forename: Guifen
authors.initials: G
authors.lastname: Li
authors.forename: Mingfu
authors.initials: M
authors.lastname: Li
authors.forename: Shifang
authors.initials: S
authors.lastname: Li
authors.forename: Shuifang
authors.initials: S
authors.lastname: Zhu
mesh_terms: D002957:Citrus / Q000821:virology; D015967:Gene Expression Regulation, Viral; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes / Q000235:genetics; D010935:Plant Diseases / Q000821:virology; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013045:Species Specificity; D059467:Transcriptome; D014772:Viroids / Q000145:classification / Q000235:genetics*
chemical_list: D015345:Oligonucleotide Probes