title: Comparison of different assays to assess human papillomavirus (HPV) type 16- and 18-specific antibodies after HPV infection and vaccination.
abstract: We compared the measurement of human papillomavirus (HPV)-specific serum antibody levels with the virus-like-particle multiplex immunoassay (VLP-MIA), competitive Luminex immunoassay (cLIA), and glutathione S-transferase (GST) L1-based MIA. Using a large panel of serum samples, these assays showed mutually good correlations for both naturally induced and vaccine-derived HPV-specific antibody levels. However, an adaptation of the GST L1-based MIA resulted in an improved correlation with both cLIA and VLP-MIA.
authors.affiliation: Laboratory for Infectious Diseases and Screening, National Institute of Public Health and the Environment, Bilthoven, the Netherlands. m.scherpenisse@amc.uva.nl
authors.forename: Mirte
authors.initials: M
authors.lastname: Scherpenisse
authors.forename: Rutger M
authors.initials: RM
authors.lastname: Schepp
authors.forename: Madelief
authors.initials: M
authors.lastname: Mollers
authors.forename: Sofie H
authors.initials: SH
authors.lastname: Mooij
authors.forename: Chris J L M
authors.initials: CJ
authors.lastname: Meijer
authors.forename: Guy A M
authors.initials: GA
authors.lastname: Berbers
authors.forename: Fiona R M
authors.initials: FR
authors.lastname: van der Klis
mesh_terms: D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral; D019411:Clinical Laboratory Techniques / Q000379:methods*; D052162:Human papillomavirus 16 / Q000276:immunology*; D052161:Human papillomavirus 18 / Q000276:immunology*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D030361:Papillomavirus Infections / Q000276:immunology*; D053918:Papillomavirus Vaccines / Q000008:administration & dosage / Q000276:immunology*; D022701:Virosomes
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D053918:Papillomavirus Vaccines; D022701:Virosomes