title: Modeling calcium-overload mediated necrosis in Drosophila.
abstract: Necrotic cell death (necrosis) occurs in many acute-onset diseases. However, our poor understanding of its mechanism has greatly limited medical interventions. Here we describe two methods to establish necrosis models in Drosophila. Our strategy is to overload calcium by expression of leaky cation channels.
authors.affiliation: State Key Lab of Biomembrane and Membrane Biotechnology, School of Life Sciences, Peking University, Beijing, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Liu
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D002118:Calcium / Q000378:metabolism*; D048430:Cell Shape; D053938:DNA Fragmentation; D004331:Drosophila melanogaster / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D005820:Genetic Testing; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008954:Models, Biological; D009336:Necrosis; D009474:Neurons / Q000166:cytology / Q000378:metabolism
chemical_list: D049452:Green Fluorescent Proteins; D002118:Calcium