title: Characterization of Alcaligenes faecalis strain AD15 indicating biocontrol activity against plant pathogens.
abstract: Bacterial strain possessing both bacteriostatic and fungistatic activity (biocontrol activity) against pathogens of cyclamen (Cyclamen sp.) was isolated from the soil in Gifu Prefecture, Japan, and characterized with respect to its taxonomic and biocontrol properties. The sequence of its 16S rRNA gene, morphology, biochemistry, and fatty acid composition demonstrated that it is a strain most closely related to Alcaligenes faecalis subsp. faecalis LMG 1229(T). The isolate was named A. faecalis strain AD15. A. faecalis AD15 produced hydroxylamine at maximum yields of 33.3±1.7 mg/L after 16 h cultivation in LB medium and 19.0±0.44 mg/L after 19 h cultivation in synthetic medium. Moreover, minimum inhibitory concentrations of hydroxylamine against the cyclamen pathogens Pantoea agglomerans and Colletotrichum gloeosporioides were 4.20±0.98 and 16.5±0.67 mg/L. These results indicated that the biocontrol activity of strain AD15 might be attributed to hydroxylamine, a metabolite in the culture medium, and it had the potential for biopesticide application.
authors.affiliation: Department of Environmental and Chemical Research, Industrial Technology Center, Gifu Prefectural Government, Hashima, Gifu 501-6064, Japan. yokoyama-shinichiro@pref.gifu.lg.jp
authors.forename: Shin-ichiro
authors.initials: S
authors.lastname: Yokoyama
authors.forename: Yoshitomi
authors.initials: Y
authors.lastname: Adachi
authors.forename: Shuichi
authors.initials: S
authors.lastname: Asakura
authors.forename: Erina
authors.initials: E
authors.lastname: Kohyama
mesh_terms: D042481:Alcaligenes faecalis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D000890:Anti-Infective Agents / Q000378:metabolism*; D000898:Antibiosis; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D020231:Colletotrichum / Q000187:drug effects; D031824:Cyclamen / Q000382:microbiology*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019811:Hydroxylamine / Q000378:metabolism*; D007564:Japan; D018625:Microscopy, Atomic Force; D008969:Molecular Sequence Data; D020636:Pantoea / Q000187:drug effects; D010572:Pest Control, Biological / Q000379:methods; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D000890:Anti-Infective Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D019811:Hydroxylamine