title: Neuronal activity-regulated pentraxin expressed in medial prefrontal cortex neurons is not necessary for extinction of heroin self-administration.
abstract: The medial prefrontal cortex (mPFC) plays a key role in extinction learning. Previously, we found that expression of a neuronal activity-regulated pentraxin (Narp) dominant-negative construct in the mPFC of mice blocked extinction of morphine-conditioned place preference. To further investigate the role of mPFC Narp in the extinction of drug seeking, we tested whether mPFC Narp is necessary for the extinction of heroin self-administration in rats. Specifically, we injected an adeno-associated viral vector expressing a dominant-negative form of Narp (NarpN) into the infralimbic region of the mPFC of rats and compared lever presses during extinction to those of rats injected with a control virus. In contrast to our previous study, we found that injection of NarpN did not affect extinction of heroin self-administration. Our findings suggest that mPFC Narp is necessary for extinction of opiate seeking in the Pavlovian-conditioned place preference paradigm but not in the operant paradigm of drug self-administration.
authors.affiliation: Department of Psychiatry and Behavioral Sciences, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA. ablouin1@jhmi.edu
authors.forename: Ashley M
authors.initials: AM
authors.lastname: Blouin
authors.forename: Anna L
authors.initials: AL
authors.lastname: Stern
authors.forename: Sungho
authors.initials: S
authors.lastname: Han
authors.forename: Florence R
authors.initials: FR
authors.lastname: Theberge
authors.forename: Chuansong
authors.initials: C
authors.lastname: Wang
authors.forename: Matthew J
authors.initials: MJ
authors.lastname: During
authors.forename: Jay M
authors.initials: JM
authors.lastname: Baraban
authors.forename: Irving M
authors.initials: IM
authors.lastname: Reti
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002097:C-Reactive Protein / Q000235:genetics / Q000378:metabolism*; D003214:Conditioning, Classical / Q000187:drug effects; D000229:Dependovirus / Q000235:genetics; D005108:Extinction, Psychological / Q000187:drug effects*; D003932:Heroin / Q000008:administration & dosage*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D009294:Narcotics / Q000008:administration & dosage*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D017397:Prefrontal Cortex / Q000166:cytology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012646:Self Administration; D013997:Time Factors; D014161:Transduction, Genetic; D000099153:Pentraxins
chemical_list: D001426:Bacterial Proteins; D002097:C-Reactive Protein; D003932:Heroin; D008164:Luminescent Proteins; D009294:Narcotics; D009419:Nerve Tissue Proteins; D000099153:Pentraxins; C054562:yellow fluorescent protein, Bacteria