title: Association between the poly(ADP-ribose) polymerase-1 gene polymorphism and advanced pelvic organ prolapse.
abstract: OBJECTIVE
  Apoptotic cell death, probably induced by oxidative stress, contributes to the development of pelvic organ prolapse. Because poly(ADP-ribose) polymerase-1 is an important mediator of cellular response to oxidative stress, genetic variations in the poly(ADP-ribose) polymerase-1 gene may play a role in the pathogenesis of pelvic organ prolapse. This study aimed to determine the association between advanced pelvic organ prolapse and Val762Ala polymorphism in the poly(ADP-ribose) polymerase-1 gene.
  
  
  METHODS
  A total of 340 women were enrolled in the study. The pelvic organ prolapse group consisted of 185 women with stage III or IV pelvic organ prolapse, whereas the control group consisted of 155 postmenopausal women with stage 0 or I pelvic organ prolapse who visited the hospital for treatment of benign gynecologic disease or routine gynecologic checkup. Genotyping of the poly(ADP-ribose) polymerase-1 Val762Ala polymorphism was performed by real-time polymerase chain reaction analysis using a TaqMan assay.
  
  
  RESULTS
  Genotype distribution in the pelvic organ prolapse group was different from that in the control group (P = 0.024). Furthermore, C-allele frequency was lower in the pelvic organ prolapse group than in the control group (P = 0.029). Women with the CC genotype had a 0.461-fold lower risk of developing advanced pelvic organ prolapse than women with the TT genotype (95% CI, 0.245-0.870; P = 0.017), and women with the C-allele had a 0.716-fold lower risk of developing advanced pelvic organ prolapse than women with the T-allele (95% CI, 0.527-0.973; P = 0.033).
  
  
  CONCLUSIONS
  These findings suggest that the poly(ADP-ribose) polymerase-1 Val762Ala polymorphism is associated with a decreased risk of advanced pelvic organ prolapse.
authors.affiliation: From the 1Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Republic of Korea; and 2Department of Obstetrics and Gynecology, Yonsei University Health System, Seoul, Republic of Korea.
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Kim
authors.forename: Eun Jae
authors.initials: EJ
authors.lastname: Kim
authors.forename: Myung Jae
authors.initials: MJ
authors.lastname: Jeon
authors.forename: Hoon
authors.initials: H
authors.lastname: Kim
authors.forename: Yeo Jung
authors.initials: YJ
authors.lastname: Moon
authors.forename: Sang Wook
authors.initials: SW
authors.lastname: Bai
mesh_terms: D000368:Aged; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D056887:Pelvic Organ Prolapse / Q000235:genetics*; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases / Q000235:genetics*; D011110:Polymorphism, Genetic; D017698:Postmenopause; D060888:Real-Time Polymerase Chain Reaction; D056910:Republic of Korea
chemical_list: C499320:PARP1 protein, human; D000071137:Poly (ADP-Ribose) Polymerase-1; D011065:Poly(ADP-ribose) Polymerases