title: Dietary selenium influences calcium release and activation of MLCK in uterine smooth muscle of rats.
abstract: We sought to elucidate the effects of different concentrations of dietary selenium on calcium ion release, MLCK levels, and muscle contraction in the uterine smooth muscle of rats. The selenium (Se) content of blood and of uterine smooth muscle tissues was detected by fluorescence spectrophotometry. Ca(2+) content was measured by atomic absorption spectroscopy. Calmodulin (CaM) and MLCK RNA and protein levels were analyzed by quantitative real-time polymerase chain reaction and Western blot, respectively. Dietary Se intake increased the Se levels in the blood and in uterine smooth muscle tissues and increased the Ca(2+) concentration in uterine smooth muscle tissues. The addition of Se also promoted CaM expression and enhanced MLCK activation in uterine smooth muscle tissues. In conclusion, Ca(2+), CaM, and MLCK were regulated by Se in uterine smooth muscle; Se plays a major role in regulating smooth muscle contraction in the uterus.
authors.affiliation: College of Veterinary Medicine, Jilin University, Changchun, Jilin Province 130062, China.
authors.forename: Mengyao
authors.initials: M
authors.lastname: Guo
authors.forename: Tingting
authors.initials: T
authors.lastname: Lv
authors.forename: Fangning
authors.initials: F
authors.lastname: Liu
authors.forename: Haiyang
authors.initials: H
authors.lastname: Yan
authors.forename: Teng
authors.initials: T
authors.lastname: Wei
authors.forename: Hua
authors.initials: H
authors.lastname: Cai
authors.forename: Wulin
authors.initials: W
authors.lastname: Tian
authors.forename: Naisheng
authors.initials: N
authors.lastname: Zhang
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
authors.forename: Guanghong
authors.initials: G
authors.lastname: Xie
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism*; D002147:Calmodulin / Q000378:metabolism; D019587:Dietary Supplements; D005260:Female; D009130:Muscle, Smooth / Q000737:chemistry / Q000378:metabolism*; D009219:Myosin-Light-Chain Kinase / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D051381:Rats; D017208:Rats, Wistar; D012643:Selenium / Q000097:blood / Q000737:chemistry / Q000378:metabolism; D015854:Up-Regulation; D014599:Uterus / Q000737:chemistry / Q000378:metabolism*
chemical_list: D002147:Calmodulin; D009219:Myosin-Light-Chain Kinase; D012643:Selenium; D002118:Calcium