title: Optimization of excitation-emission band-pass filter for visualization of viable bacteria distribution on the surface of pork meat.
abstract: A novel method of optically reducing the dimensionality of an excitation-emission matrix (EEM) by optimizing the excitation and emission band-pass filters was proposed and applied to the visualization of viable bacteria on pork. Filters were designed theoretically using an EEM data set for evaluating colony-forming units on pork samples assuming signal-to-noise ratios of 100, 316, or 1000. These filters were evaluated using newly measured EEM images. The filters designed for S/N = 100 performed the best and allowed the visualization of viable bacteria distributions. The proposed method is expected to be a breakthrough in the application of EEM imaging.
authors.affiliation: Department of Computer Science and Engineering, Toyohashi University of Technology, Toyohashi 441-8580, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Nishino
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Nakamura
authors.forename: Mizuki
authors.initials: M
authors.lastname: Tsuta
authors.forename: Masatoshi
authors.initials: M
authors.lastname: Yoshimura
authors.forename: Junichi
authors.initials: J
authors.lastname: Sugiyama
authors.forename: Shigeki
authors.initials: S
authors.lastname: Nakauchi
mesh_terms: D000818:Animals; D001419:Bacteria / Q000302:isolation & purification*; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology / Q000379:methods*; D008460:Meat / Q000382:microbiology*; D008856:Microscopy, Fluorescence / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013552:Swine