title: Immunohistochemical characterization of axonal sprouting in mice.
abstract: OBJECTIVE
  Transgenic manipulation of mouse physiology facilitates the preclinical study of genetic risk factors, neural plasticity, and reactive processes accompanying Alzheimer's disease. Alternatively, entorhinal cortex lesions (ECLs) model pathophysiological denervation and axonal sprouting in rat. Given reports of anatomical differences between the mouse and rat hippocampus, application of the ECL paradigm to transgenic mice first requires comprehensive characterization of axonal sprouting in the wild-type.
  
  
  METHODS
  Adult male C57BL/6 mice sustained unilateral transections of the perforant pathway. Subjects were sacrificed at 1, 4, 10, 18, and 28 days postlesion, and hippocampal sections were stained for AChE, the postsynaptic terminal marker drebrin, and the presynaptic terminal proteins SNAP-25, GAP-43, synapsin, and synaptophysin. To examine synaptic turnover and reinnervation, ipsilateral-to-contralateral staining densities were determined within the dentate molecular layer, and shrinkage-corrected ratios were compared to 28 day-yoked sham cases.
  
  
  RESULTS
  At 28 days postlesion, ipsilateral terminal marker densities exhibited significant depression. In contrast, qualitative analyses at earlier time points suggested altered AChE staining patterns and increased SNAP-25 and synapsin immunoreactivity in the inner molecular layer (IML) of the dentate gyrus.
  
  
  CONCLUSIONS
  C57BL/6 mice exhibit synaptic reorganization following perforant path transections. The IML may provide a key target for evaluation and intervention in ECL mouse models.
authors.affiliation: Neuroscience Program, Davidson College, Davidson, NC, USA.
authors.forename: Erin J
authors.initials: EJ
authors.lastname: Feeney
authors.forename: Diane
authors.initials: D
authors.lastname: Stephenson
authors.forename: Robin
authors.initials: R
authors.lastname: Kleiman
authors.forename: Susan
authors.initials: S
authors.lastname: Bove
authors.forename: Courtney
authors.initials: C
authors.lastname: Cron
authors.forename: Lara
authors.initials: L
authors.lastname: Moody
authors.forename: Mercedes
authors.initials: M
authors.lastname: Robinson
authors.forename: Julio J
authors.initials: JJ
authors.lastname: Ramirez
mesh_terms: D000818:Animals; D001369:Axons / Q000737:chemistry* / Q000502:physiology*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009473:Neuronal Plasticity / Q000502:physiology*; D013569:Synapses / Q000737:chemistry* / Q000502:physiology*