title: Peroxidase-like activity of amino-functionalized magnetic nanoparticles and their applications in immunoassay.
abstract: Amino-functionalized Fe3O4 magnetic nanoparticles with high peroxidase-like activity (MNPs-NH2) are reported in this work. The peroxidase-like activity is approximately the same as naked Fe3O4 magnetic nanoparticles (MNPs). MNPs-NH2 displayed much better thermal stability and pH tolerance than horseradish peroxidase (HRP). The optimal pH and temperature are approximately pH 4 and 40 °C, respectively. We showed that the MNPs-NH2 were successfully applied in a double-antibody sandwich "enzyme"-linked immunosorbent assay to replace of HRP, in which MNPs-NH2 were not only separation carriers, but also detection indicator.
authors.affiliation: School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing 100083, China.
authors.forename: Mingzhu
authors.initials: M
authors.lastname: Yang
authors.forename: Yueping
authors.initials: Y
authors.lastname: Guan
authors.forename: Yu
authors.initials: Y
authors.lastname: Yang
authors.forename: Tingting
authors.initials: T
authors.lastname: Xia
authors.forename: Wubin
authors.initials: W
authors.lastname: Xiong
authors.forename: Ning
authors.initials: N
authors.lastname: Wang
authors.forename: Chen
authors.initials: C
authors.lastname: Guo
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D002384:Catalysis; D006063:Chorionic Gonadotropin / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007118:Immunoassay; D008280:Magnetics; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology; D009195:Peroxidase / Q000737:chemistry*; D013499:Surface Properties; D013696:Temperature
chemical_list: D001672:Biocompatible Materials; D006063:Chorionic Gonadotropin; D009195:Peroxidase