title: Relation between dietary acrylamide exposure and biomarkers of internal dose in Canadian teenagers.
abstract: Acrylamide (AA) is a probable human carcinogen found in several foods. Little information is available regarding exposure of adolescents, a subgroup potentially consuming more AA-rich foods. We investigated the relationship between dietary AA intake and levels of biomarkers of exposure (urinary metabolites and hemoglobin adducts) in 195 non-smoking teenagers of Montreal Island aged 10-17 years. Dietary habits and personal characteristics were documented by questionnaire. AA and its metabolites were quantified in 12-h urine collections by LC-MS/MS. Hemoglobin adducts from 165 blood samples were also analyzed by LC-MS/MS. Most prevalent urinary metabolites were NACP and NACP-S, with respective geometric mean concentrations of 31.2 and 14.2 μmol/mol creatinine. Geometric mean concentrations of AAVal and GAVal (hemoglobin adducts of AA and glycidamide (GA) with N-terminal valine residues) were 45.4 and 45.6 pmol/g globin, respectively. AA intake during the 2 days before urine collection was a significant predictor of NACP+NACP-S urinary concentrations (P<0.0001). AA intakes during the month before blood collection (P<0.0001) and passive smoking (P<0.05) were associated with adduct levels. Levels of hemoglobin adducts were above biomonitoring equivalent values corresponding to a 1 × 10(-4) excess cancer risk, which may indicate the need to reduce AA exposure in the population.
authors.affiliation: 1] Institut national de santé publique du Québec, Québec, Canada [2] Université Laval, Québec, Canada.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Brisson
authors.affiliation: 1] Institut national de santé publique du Québec, Québec, Canada [2] Université Laval, Québec, Canada.
authors.forename: Pierre
authors.initials: P
authors.lastname: Ayotte
authors.affiliation: Institut national de santé publique du Québec, Québec, Canada.
authors.forename: Louise
authors.initials: L
authors.lastname: Normandin
authors.affiliation: Institut national de santé publique du Québec, Québec, Canada.
authors.forename: Éric
authors.initials: É
authors.lastname: Gaudreau
authors.affiliation: Institut national de santé publique du Québec, Québec, Canada.
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Bienvenu
authors.affiliation: RTI International, Research Triangle Park, North Carolina, USA.
authors.forename: Timothy R
authors.initials: TR
authors.lastname: Fennell
authors.affiliation: Institut national de santé publique du Québec, Québec, Canada.
authors.forename: Carole
authors.initials: C
authors.lastname: Blanchet
authors.affiliation: Institut national de santé publique du Québec, Québec, Canada.
authors.forename: Denise
authors.initials: D
authors.lastname: Phaneuf
authors.affiliation: Santé Canada, Longueuil, Québec, Canada.
authors.forename: Caroline
authors.initials: C
authors.lastname: Lapointe
authors.affiliation: Santé Canada, Longueuil, Québec, Canada.
authors.forename: Yvette
authors.initials: Y
authors.lastname: Bonvalot
authors.affiliation: Institut national de santé publique du Québec, Québec, Canada.
authors.forename: Michelle
authors.initials: M
authors.lastname: Gagné
authors.affiliation: Institut national de santé publique du Québec, Québec, Canada.
authors.forename: Marilène
authors.initials: M
authors.lastname: Courteau
authors.affiliation: RTI International, Research Triangle Park, North Carolina, USA.
authors.forename: Rodney W
authors.initials: RW
authors.lastname: Snyder
authors.affiliation: Département de santé environnementale et santé au travail, Chaire d'analyse et de gestion des risques toxicologiques, Institut de recherche en santé publique, Université de Montréal, Montréal, Québec, Canada.
authors.forename: Michèle
authors.initials: M
authors.lastname: Bouchard
mesh_terms: D020106:Acrylamide / Q000633:toxicity*; D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000378:metabolism*; D002170:Canada; D004032:Diet; D004305:Dose-Response Relationship, Drug; D004781:Environmental Exposure; D005260:Female; D006801:Humans; D008875:Middle Aged
chemical_list: D015415:Biomarkers; D020106:Acrylamide