title: Microbial monitoring by molecular tools of an upflow anaerobic filter treating abattoir wastewaters.
abstract: The performance of anaerobic digestion of abattoir wastewaters (AW) in an upflow anaerobic filter (UAF) was investigated under mesophilic (37°C) and thermophilic (55°C) conditions. The effects of increasing temperature on the performance of the UAF and on the dynamics of the microbial community of the anaerobic sludge were studied. The results showed that chemical oxygen demand (COD) removal efficiency of 90% was achieved for organic loading rates (OLRs) up to 4.5g CODL(-1)d(-1) in mesophilic conditions, while in thermophilic conditions, the highest OLRs of 9 g CODL(-1)d(-1) led to the efficiency of 72%. The use of molecular and microbiological methods to recover microbial populations involved in this process showed that fermentative bacteria were the prominent members of the sludge microbial community. Three novel strains were identified as Macellibacteroides fermentans, Desulfotomaculum peckii and Defluviitalea saccharophila.
authors.affiliation: Université de Carthage, Laboratoire d'Ecologie et de Technologie Microbienne, INSAT, B.P. 676, 1080 Tunis, Tunisia.
authors.forename: Hana
authors.initials: H
authors.lastname: Gannoun
authors.forename: Eltaief
authors.initials: E
authors.lastname: Khelifi
authors.forename: Ilhem
authors.initials: I
authors.lastname: Omri
authors.forename: Linda
authors.initials: L
authors.lastname: Jabari
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Fardeau
authors.forename: Hassib
authors.initials: H
authors.lastname: Bouallagui
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Godon
authors.forename: Moktar
authors.initials: M
authors.lastname: Hamdi
mesh_terms: D000003:Abattoirs; D000693:Anaerobiosis; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D001483:Base Sequence; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors; D017931:DNA Primers; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012722:Sewage / Q000382:microbiology; D062065:Wastewater; D014871:Water Microbiology
chemical_list: D017931:DNA Primers; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D062065:Wastewater
keywords: Abattoir wastewater; Anaerobic digestion; Mesophilic; Microbial diversity; Thermophilic