title: Photobleaching lifetimes of cyanine fluorophores used for single-molecule Förster resonance energy transfer in the presence of various photoprotection systems.
abstract: Lengthening smFRET lifetimes: We investigated various photoprotection system combinations to find the combination that optimally extended the photobleach lifetime of a Cy3/Cy5 smFRET pair attached to a DNA hairpin in a single-molecule environment. We found that the glucose/glucose oxygen-scavenging solution in combination with redox-based photostabilization solutions yielded the longest average photobleaching lifetimes.
authors.affiliation: Department of Chemistry, Rice University, 6100 Main St. Houston, TX 77005, USA.
authors.forename: David
authors.initials: D
authors.lastname: Cooper
authors.forename: Heui
authors.initials: H
authors.lastname: Uhm
authors.forename: Lawrence J
authors.initials: LJ
authors.lastname: Tauzin
authors.forename: Nitesh
authors.initials: N
authors.lastname: Poddar
authors.forename: Christy F
authors.initials: CF
authors.lastname: Landes
mesh_terms: D002232:Carbocyanines / Q000737:chemistry*; D004247:DNA / Q000737:chemistry; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry; D005947:Glucose / Q000737:chemistry; D010084:Oxidation-Reduction; D038761:Photobleaching
chemical_list: D002232:Carbocyanines; D005456:Fluorescent Dyes; C089187:cyanine dye 3; C085321:cyanine dye 5; D004247:DNA; D005947:Glucose