title: Study of Ni uptake and its compartmentalization in Ni(r), Pd(r) and Ni(s)/ Pd(s) strain of Nostoc muscorum.
abstract: A Ni(r)20 and its analog Pd(r)20 mutant strain was obtained from its Ni(s)/Pd(s) (nickel and palladium sensitive) strain of Nostoc muscorum. Ni(r)20 and Pd(r)20 mutant strain of Nostoc muscorum was resistant to 20 microM Ni and Pd. Ni uptake was observed in Ni(s)/Pd(s), Ni(r)20 and Pd(r)20 mutant strain of Nostoc muscorum by treating with 120 microM Ni saturating concentration. Ni uptake was two fold more in Ni(r)20 cells (31.0 nmol microg(-1) protein) than the Ni(s) cells, however Pd(r)20 strain took less Ni (9.31 nmol microg(-1) protein). Phosphate uptake was also investigated to determine the poly phosphate synthesis. The cells of Ni(s)/ Pd(s), Ni(r)20 and Pd(r)20 strain were treated for four hours in 2mM K2HPO4 exposure. Phosphate uptake in Ni(r)20 strain was 1.8 fold more over Ni(s)/Pd(s), and was least in Pd(r)20 strain. Polyphosphate level was determined to better understand the Ni transport in Ni(s), Ni(r)20, Pd(r)20 strain. Polyp level was increased two fold in Ni(r) strain and least in Pd(r) strain followed by Ni(s) strain. Results based on the Ni distribution pattern in Ni(s), Ni(r)20, Pd(r)20 are the evidence that poly p is the main metal sink.
authors.affiliation: Department of Botany, Banaras Hindu University, Varanasi-221 005, India.
authors.forename: Asha Lata
authors.initials: AL
authors.lastname: Singh
mesh_terms: D009532:Nickel / Q000378:metabolism*; D050502:Nostoc muscorum / Q000378:metabolism*; D010165:Palladium / Q000378:metabolism; D013045:Species Specificity
chemical_list: D010165:Palladium; D009532:Nickel