title: Exercise training upregulates nitric oxide synthases in the kidney of rats with chronic heart failure.
abstract: There is an interaction between heart and kidney diseases, which is a condition termed cardiorenal syndrome. Exercise training has cardioprotective effects, involving upregulation of endothelial (e) nitric oxide synthase (NOS) in the cardiovascular system. However, the effects of exercise training on NOS in the kidney with heart disease are unknown. The aim of the present study was to investigate whether exercise training upregulates NOS in the kidney, left ventricle and aorta of rats with chronic heart failure (CHF). Male Sprague-Dawley rats underwent left coronary artery ligation (LCAL) to induce CHF and were randomly assigned to sedentary or treadmill exercise groups 4 weeks after LCAL. Three days after exercising for 4 weeks, urine samples were collected for 24 h and blood samples were collected following decapitation. Nitric oxide synthase activity and protein expression were examined. Significant interactions between CHF and exercise training were observed on parameters of cardiac and renal function. Exercise training improved cardiac function, decreased plasma B-type natriuretic peptide levels, decreased urinary albumin excretion and increased creatinine clearance in CHF rats. Nitric oxide synthase activity, eNOS expression and neuronal (n) NOS expression were significantly decreased in the left ventricle and kidney of CHF rats. Exercise training significantly increased NOS activity and eNOS and nNOS expression. Upregulation of NOS in the kidney and left ventricle may contribute, in part, to the renal and cardiac protective effects of exercise training in cardiorenal syndrome in CHF rats.
authors.affiliation: Department of Internal Medicine and Rehabilitation Science, Tohoku University Graduate School of Medicine, Sendai, Japan. dairyoub4@hotmail.co.jp
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ito
authors.forename: Osamu
authors.initials: O
authors.lastname: Ito
authors.forename: Nobuyoshi
authors.initials: N
authors.lastname: Mori
authors.forename: Pengyu
authors.initials: P
authors.lastname: Cao
authors.forename: Chihiro
authors.initials: C
authors.lastname: Suda
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Muroya
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Hao
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Shimokawa
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kohzuki
mesh_terms: D000818:Animals; D001011:Aorta / Q000378:metabolism; D006333:Heart Failure / Q000378:metabolism*; D006352:Heart Ventricles / Q000378:metabolism; D007668:Kidney / Q000378:metabolism*; D052248:Nitric Oxide Synthase Type I / Q000235:genetics* / Q000378:metabolism*; D052250:Nitric Oxide Synthase Type III / Q000235:genetics* / Q000378:metabolism*; D010805:Physical Conditioning, Animal / Q000502:physiology*; D051381:Rats; D015854:Up-Regulation / Q000235:genetics*
chemical_list: D052248:Nitric Oxide Synthase Type I; D052250:Nitric Oxide Synthase Type III; C496316:Nos3 protein, rat
keywords: chronic heart failure; exercise; kidney; nitric oxide synthase