title: Establishment of a new detection system for the dimerization of IRE1α by BiFC assay.
abstract: We developed a new detection system for the activation of an endoplasmic reticulum (ER) stress sensor, inositol requiring kinase 1 α (IRE1α), by evaluating dimerization of it by bimolecular fluorescence complementation (BiFC) assay. By detecting the fluorescence derived from the reconstituted cerulean, this assay system enabled us to distinguish the activation behaviors of IRE1α as to ER stress-inducing compounds.
authors.affiliation: Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University, Yokohama, Japan.
authors.forename: Satoko
authors.initials: S
authors.lastname: Shinjo
authors.forename: Etsu
authors.initials: E
authors.lastname: Tashiro
authors.forename: Masaya
authors.initials: M
authors.lastname: Imoto
mesh_terms: D000818:Animals; D019281:Dimerization; D004721:Endoplasmic Reticulum / Q000235:genetics*; D059865:Endoplasmic Reticulum Stress; D004722:Endoribonucleases / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D005453:Fluorescence; D006367:HeLa Cells; D006801:Humans; D007294:Inositol / Q000737:chemistry / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000737:chemistry* / Q000302:isolation & purification* / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology
chemical_list: D007294:Inositol; C528229:ERN1 protein, human; D017346:Protein Serine-Threonine Kinases; D004722:Endoribonucleases