title: CDC20 overexpression predicts a poor prognosis for patients with colorectal cancer.
abstract: BACKGROUND
  The cell division cycle 20 homolog (CDC20) is an essential cofactor of the anaphase-promoting complex (APC/C). CDC20 overexpression has been detected in many types of human cancers; however, its clinical role in colorectal cancer remains unknown.
  
  
  METHODS
  Western blotting and immunohistochemistry were used to compare CDC20 expression in adjacent non-cancerous, cancerous and liver metastatic tissues as well as in colon cancer cell lines and normal colon epithelial cell lines. Additionally, the correlation of CDC20 expression with patient clinical parameters and its diagnostic value were statistically analyzed.
  
  
  RESULTS
  CDC20 was overexpressed in colon cancer cell lines/primary cancer tissues compared with normal colon epithelial cell lines/adjacent noncancerous tissue samples. Interestingly, CDC20 expression was further increased in metastatic liver tissues. CDC20 protein expression was significantly correlated with clinical stage (P = 0.008), N classification (P = 0.020), M classification (P = 0.013) and pathologic differentiation (P = 0.008). Patients with higher CDC20 expression had a shorter overall survival than those with lower CDC20 expression. Univariate and multivariate analyses indicated that CDC20 expression was an independent prognostic factor (P < 0.001).
  
  
  CONCLUSIONS
  CDC20 may serve as a potential prognostic biomarker of human colorectal cancer.
authors.affiliation: State Key Laboratory of Oncology in Southern China, Guangzhou 510060, China.
authors.forename: Wen-jing
authors.initials: WJ
authors.lastname: Wu
authors.forename: Kai-shun
authors.initials: KS
authors.lastname: Hu
authors.forename: De-shen
authors.initials: DS
authors.lastname: Wang
authors.forename: Zhao-lei
authors.initials: ZL
authors.lastname: Zeng
authors.forename: Dong-sheng
authors.initials: DS
authors.lastname: Zhang
authors.forename: Dong-liang
authors.initials: DL
authors.lastname: Chen
authors.forename: Long
authors.initials: L
authors.lastname: Bai
authors.forename: Rui-hua
authors.initials: RH
authors.lastname: Xu
mesh_terms: D000328:Adult; D000368:Aged; D064199:Cdc20 Proteins / Q000378:metabolism*; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000473:pathology / Q000556:secondary; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009362:Neoplasm Metastasis; D011379:Prognosis; D016896:Treatment Outcome
chemical_list: D064199:Cdc20 Proteins; C577488:CDC20 protein, human