title: Genomic organization of a novel partitivirus from the phytopathogenic fungus Ustilaginoidea virens.
abstract: From the plant pathogen Ustilaginoidea virens, four double-stranded RNA (dsRNA) segments designated Uv-dsRNA1, -2, -3, and -4 were isolated, cloned, and sequenced. Uv-dsRNA1 (1775 bp) and -2 (1588 bp) potentially encode an RNA-dependent RNA polymerase (RdRp) and a viral coat protein (CP), respectively. Since the RdRp and CP sequences encoded by Uv-dsRNA1 and -2, respectively, are most closely related to, but clearly distinct from, those of viruses of the genus Partitivirus, they appear to be the two genome segments of a new partitivirus, for which the name Ustilaginoidea virens partitivirus 1 is proposed. In contrast, Uv-dsRNA3 (1352 bp) did not share significant sequence similarity with GenBank sequences, and the ORF of Uv-dsRNA4 (1119 bp) was only 32 % identical to a functionally unknown protein (GaRVMS2s3gp1) encoded by Gremmeniella abietina RNA virus MS2.
authors.affiliation: Department of Plant Pathology, College of Plant Science and Technology and the Key Lab of Crop Disease Monitoring and Safety Control in Hubei Province, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Zhang
authors.forename: Yinhui
authors.initials: Y
authors.lastname: Jiang
authors.forename: Junbin
authors.initials: J
authors.lastname: Huang
authors.forename: Wubei
authors.initials: W
authors.lastname: Dong
mesh_terms: D001483:Base Sequence; D036022:Capsid Proteins / Q000235:genetics; D016679:Genome, Viral / Q000235:genetics*; D023281:Genomics; D006999:Hypocreales / Q000472:pathogenicity / Q000821:virology*; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000382:microbiology*; D012328:RNA Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012330:RNA, Double-Stranded / Q000737:chemistry / Q000235:genetics; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics
chemical_list: D036022:Capsid Proteins; D012330:RNA, Double-Stranded; D014764:Viral Proteins; D012324:RNA-Dependent RNA Polymerase