title: [Classification and RAPD analysis of cultivate varieties of Lonicera japonica cultivated in Xinmi].
abstract: OBJECTIVE
  To study the classification and genetic relationship among difference cultivated varieties of Lonicera japonica cultivated in Xinmi.
  
  
  METHODS
  Observed the biological characteristics of Lonicera japonica cultivated in Xinmi and visited farmers; Genetic diversity was analyzed by RAPD molecular biology techniques.
  
  
  RESULTS
  Classified the cultivated varieties and set up an index key. RAPD fingerprint was established, 10 primers were chosen. A total of 60 DNA fragments, among which 50 bands was polymorphic, accounting for 83.33% which indicated the genetic diversity of the samples was good.
  
  
  CONCLUSIONS
  Genetic relationship among different cultivated varieties are determined which provide scientific basis for resources protection, development of Lonicera japonica cultivated in Xinmi and its quality evaluation.
authors.affiliation: Pharmacy College of Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China. wllywlly2004@163.com
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Wang
authors.forename: Jin-Ge
authors.initials: JG
authors.lastname: Zhang
authors.forename: Sui-Qing
authors.initials: SQ
authors.lastname: Chen
mesh_terms: D002681:China; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D035264:Flowers / Q000033:anatomy & histology / Q000235:genetics; D014644:Genetic Variation; D031239:Lonicera / Q000033:anatomy & histology / Q000145:classification* / Q000235:genetics*; D010802:Phylogeny; D018515:Plant Leaves / Q000033:anatomy & histology / Q000235:genetics; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*
chemical_list: D017931:DNA Primers; D018744:DNA, Plant