title: Glycoform remodeling generates a synthetic T cell phenotype.
abstract: The glycan of specific proteins can dictate the response of cells to stimuli, and thus their phenotype. We describe a chemical strategy to modify the cellular glycoform of T cells, which resulted in a modified cellular response. Our data indicate that chemical modification of the phosphatase CD45 is responsible for the observed differences in response to receptor cross-linking. By increasing the content of galactose epitopes in the glycocalyx of a lymphoma cell line, we were able to increase the response of the cell to lectin stimulation through the glycoprotein receptor, CD45. The method described here exploits metabolic labeling of a cell to reprogram the cellular response to external stimuli though changes in the number of lectin binding sites on the cell surface.
authors.affiliation: Alberta Glycomics Centre, Department of Chemistry, University of Alberta, Edmonton, Alberta T6G 2G2, Canada.
authors.forename: Chunxia
authors.initials: C
authors.lastname: Zou
authors.forename: Ravi S
authors.initials: RS
authors.lastname: Loka
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Christopher W
authors.initials: CW
authors.lastname: Cairo
mesh_terms: D002478:Cells, Cultured; D018984:Epitopes, T-Lymphocyte / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D017493:Leukocyte Common Antigens / Q000737:chemistry / Q000378:metabolism*; D015394:Molecular Structure; D010641:Phenotype; D011134:Polysaccharides / Q000737:chemistry / Q000378:metabolism*
chemical_list: D018984:Epitopes, T-Lymphocyte; D011134:Polysaccharides; D017493:Leukocyte Common Antigens