title: [Research of inhibition of compound gan shu kang on hepatitis B virus].
abstract: OBJECTIVE
  To study the anti-HBV effect in vitro of each extract from compound Gan Shu Kang.
  
  
  METHODS
  The toxicity was investigated with cytopathic effect (CPE) by enzyme linked immuno-adsorbed assay (ELISA), the inhibitory effect of each extract on HBsAg and HBeAg secreted by 2215 cell line infected by HBV DNA was evaluated.
  
  
  RESULTS
  The half of the toxic concentration (TC50) of petroleum ether, ethyl acetate and n-butanol was 125, 375 and 62.5 microg/mL, respectively. The maximum nontoxic concentration (TC0) was 62.5, 125 and 31.3 microg/mL, respectively. The medium effective concentration (IC50) of petroleum ether and ethyl acetate on 2215 cellular HBsAg express the inhibitory effect was 48.6 and 14.0 microg/mL, HBeAg was 52.4 and 19.7 microg/mL, while the IC50 of n-butanol for HBsAg and HBeAg was more than 125 microg/mL. The therapeutic index (TI) of petroleum ether was 2.57 and 2.43, ethyl acetate was 26.7 and 19.04, n-butanol was both less than 2.
  
  
  CONCLUSIONS
  The anti-HBV effect of ethyl acetate extract of compound Gan Shu Kang is better than that of petroleum ether, while the n-butanol extract shows no effect.
authors.affiliation: Wuhan University of Technology, Wuhan 430070, China. lhl2345@126.com
authors.forename: Hong-Lin
authors.initials: HL
authors.lastname: Li
authors.forename: Tao-Ying
authors.initials: TY
authors.lastname: Cheng
authors.forename: Ping
authors.initials: P
authors.lastname: Lu
authors.forename: Ya-Li
authors.initials: YL
authors.lastname: Cheng
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhao
mesh_terms: D020001:1-Butanol; D000085:Acetates; D000998:Antiviral Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004279:DNA, Viral / Q000235:genetics; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D056945:Hep G2 Cells; D006514:Hepatitis B Surface Antigens / Q000378:metabolism; D006513:Hepatitis B e Antigens / Q000378:metabolism; D006515:Hepatitis B virus / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D020128:Inhibitory Concentration 50; D010946:Plants, Medicinal / Q000737:chemistry*; D014162:Transfection
chemical_list: D000085:Acetates; D000998:Antiviral Agents; D004279:DNA, Viral; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; C007650:ethyl acetate; D020001:1-Butanol