title: Distinct neuroblastoma-associated alterations of PHOX2B impair sympathetic neuronal differentiation in zebrafish models.
abstract: Heterozygous germline mutations and deletions in PHOX2B, a key regulator of autonomic neuron development, predispose to neuroblastoma, a tumor of the peripheral sympathetic nervous system. To gain insight into the oncogenic mechanisms engaged by these changes, we used zebrafish models to study the functional consequences of aberrant PHOX2B expression in the cells of the developing sympathetic nervous system. Allelic deficiency, modeled by phox2b morpholino knockdown, led to a decrease in the terminal differentiation markers th and dbh in sympathetic ganglion cells. The same effect was seen on overexpression of two distinct neuroblastoma-associated frameshift mutations, 676delG and K155X - but not the R100L missense mutation - in the presence of endogenous Phox2b, pointing to their dominant-negative effects. We demonstrate that Phox2b is capable of regulating itself as well as ascl1, and that phox2b deficiency uncouples this autoregulatory mechanism, leading to inhibition of sympathetic neuron differentiation. This effect on terminal differentiation is associated with an increased number of phox2b(+), ascl1(+), elavl3(-) cells that respond poorly to retinoic acid. These findings suggest that a reduced dosage of PHOX2B during development, through either a heterozygous deletion or dominant-negative mutation, imposes a block in the differentiation of sympathetic neuronal precursors, resulting in a cell population that is likely to be susceptible to secondary transforming events.
authors.affiliation: Department of Pediatric Oncology, Dana-Farber Cancer Institute, Division of Hematology/Oncology, Boston Children's Hospital, Harvard Medical School, Boston, MA, USA.
authors.forename: Desheng
authors.initials: D
authors.lastname: Pei
authors.forename: William
authors.initials: W
authors.lastname: Luther
authors.forename: Wenchao
authors.initials: W
authors.lastname: Wang
authors.forename: Barry H
authors.initials: BH
authors.lastname: Paw
authors.forename: Rodney A
authors.initials: RA
authors.lastname: Stewart
authors.forename: Rani E
authors.initials: RE
authors.lastname: George
mesh_terms: D000818:Animals; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics*; D051959:ELAV Proteins / Q000235:genetics / Q000378:metabolism; D000067778:ELAV-Like Protein 3; D018507:Gene Expression Regulation, Developmental; D020022:Genetic Predisposition to Disease; D006579:Heterozygote; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D009447:Neuroblastoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D055495:Neurogenesis; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D013564:Sympathetic Nervous System / Q000166:cytology / Q000473:pathology; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015027:Zebrafish / Q000235:genetics / Q000254:growth & development; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D051959:ELAV Proteins; D000067778:ELAV-Like Protein 3; D018398:Homeodomain Proteins; D014157:Transcription Factors; D029961:Zebrafish Proteins; C468127:Ascl1a protein, zebrafish; C109220:NBPhox protein; C088635:elavl3 protein, zebrafish