title: Inhibition of bacterial motility and spreading via release of cranberry derived materials from silicone substrates.
abstract: The motility of bacteria plays a key role in their colonization of surfaces during infection. Derivatives of cranberry fruit have been shown to interfere with bacterial motility. Herein, we report on the incorporation of cranberry derived materials (CDMs) into silicone substrates with the aim of impairing bacterial pathogen motility and spreading on the substrate surface. The release of CDMs from the silicone substrates when soaking in an aqueous medium was quantified for a period of 24h. Next, we showed that CDMs released from two silicone substrates remain bioactive as they downregulate the expression of the flagellin gene of two key uropathogens - Escherichia coli CFT073 and Proteus mirabilis HI4320. Furthermore, we demonstrate that CDM-modified silicone inhibits the swarming motility of P. mirabilis, an aggressive swarmer. The bioactive, CDM-modified substrates can find broad applications in the medical device and food industries where the impairment of bacterial colonization of surfaces is of paramount importance.
authors.affiliation: Department of Chemical Engineering, McGill University, 3610 University Street, Montreal, Quebec H3A 2B2, Canada.
authors.forename: Michelle
authors.initials: M
authors.lastname: Chan
authors.forename: Gabriela
authors.initials: G
authors.lastname: Hidalgo
authors.forename: Bahareh
authors.initials: B
authors.lastname: Asadishad
authors.forename: Sergio
authors.initials: S
authors.lastname: Almeida
authors.forename: Naser
authors.initials: N
authors.lastname: Muja
authors.forename: Maziar Shah
authors.initials: MS
authors.lastname: Mohammadi
authors.forename: Showan N
authors.initials: SN
authors.lastname: Nazhat
authors.forename: Nathalie
authors.initials: N
authors.lastname: Tufenkji
mesh_terms: D000818:Animals; D004175:Diptera / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects* / Q000378:metabolism; D010316:Particle Size; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011513:Proteus mirabilis / Q000187:drug effects / Q000378:metabolism; D012828:Silicones / Q000737:chemistry / Q000494:pharmacology*; D013499:Surface Properties
chemical_list: D010936:Plant Extracts; D012828:Silicones