title: Expression of miR-126 suppresses migration and invasion of colon cancer cells by targeting CXCR4.
abstract: A previous study demonstrated that miR-126 expression was significantly downregulated in highly metastatic colon cancer cells. This study was to investigate the biological function of miR-126 and its regulation of target genes in colon cancer cells. Quantitative PCR was used to detect miR-126 expression in colon cancer SW480 and SW620 cells. MTT assay was to measure the changed cell viability after miR-126 mimics transfection. Wound healing and Transwell migration and invasion assays measured capacity of tumor cell migration and invasion of SW480 and SW620 cells after miR-126 transfection. Luciferase reporter assay and Western blot were used to assess both transcriptional and expression levels of one of the miR-126 target genes (i.e., CXCR4). Levels of miR-126 expression were lower in colon cancer SW480 and SW620 cells than in the adjacent normal epithelial tissues (P < 0.05). Transfection of miR-126 mimics significantly reduced colon cancer cell viability compared to NC cells (P < 0.05). The wound healing and Transwell migration and invasion assays showed that miR-126 mimics inhibited SW480 and SW620 cell migration and invasion capacity. Bioinformatics predicted that CXCR4 is one of the miR-126 target genes. Indeed, luciferase reporter assay and Western blot confirmed that CXCR4 is a miR-126 target gene. Expression of miR-126 inhibited colon cancer cell viability and reduced tumor cell migration and invasion capacity by its negative regulation of CXCR4 expression.
authors.affiliation: Department of Gastroenterology, The Xiangtan Central Hospital, Xiangtan, China.
authors.forename: Zeng
authors.initials: Z
authors.lastname: Li
authors.forename: Nan
authors.initials: N
authors.lastname: Li
authors.forename: Minghua
authors.initials: M
authors.lastname: Wu
authors.forename: Xiayu
authors.initials: X
authors.lastname: Li
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Luo
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Wang
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D002470:Cell Survival / Q000235:genetics; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology*; D019295:Computational Biology; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D009361:Neoplasm Invasiveness; D019718:Receptors, CXCR4 / Q000378:metabolism*; D014162:Transfection; D014945:Wound Healing / Q000235:genetics
chemical_list: C527276:CXCR4 protein, human; C527111:MIRN126 microRNA, human; D035683:MicroRNAs; D019718:Receptors, CXCR4