title: Detection of osteogenic differentiation by differential mineralized matrix production in mesenchymal stromal cells by Raman spectroscopy.
abstract: Mesenchymal stromal cells (MSCs) hold great potential in skeletal tissue engineering and regenerative medicine. However, conventional methods that are used in molecular biology to evaluate osteogenic differentiation of MSCs require a relatively large amount of cells. Cell lysis and cell fixation are also required and all these steps are time-consuming. Therefore, it is imperative to develop a facile technique which can provide real-time information with high sensitivity and selectivity to detect the osteogenic maturation of MSCs. In this study, we use Raman spectroscopy as a biosensor to monitor the production of mineralized matrices during osteogenic induction of MSCs. In summary, Raman spectroscopy is an excellent biosensor to detect the extent of maturation level during MSCs-osteoblast differentiation with a non-disruptive, real-time and label free manner. We expect that this study will promote further investigation of stem cell research and clinical applications.
authors.affiliation: Institute of Biophotonics, National Yang-Ming University, Taipei, Taiwan.
authors.forename: Pei-San
authors.initials: PS
authors.lastname: Hung
authors.forename: Yi-Chun
authors.initials: YC
authors.lastname: Kuo
authors.forename: He-Guei
authors.initials: HG
authors.lastname: Chen
authors.forename: Hui-Hua Kenny
authors.initials: HH
authors.lastname: Chiang
authors.forename: Oscar Kuang-Sheng
authors.initials: OK
authors.lastname: Lee
mesh_terms: D002130:Calcium Phosphates / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000235:genetics; D002454:Cell Differentiation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics; D017886:Durapatite / Q000378:metabolism; D020869:Gene Expression Profiling; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D010012:Osteogenesis; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013059:Spectrum Analysis, Raman / Q000379:methods*; D023822:Tissue Engineering / Q000379:methods
chemical_list: D002130:Calcium Phosphates; D015815:Cell Adhesion Molecules; D024042:Collagen Type I; D050659:Core Binding Factor Alpha 1 Subunit; C512835:POSTN protein, human; C022045:octacalcium phosphate; D017886:Durapatite; C020243:calcium phosphate; C018392:tricalcium phosphate