title: Mammalian galectins bind galactoseβ1-4fucose disaccharide, a unique structural component of protostomial N-type glycoproteins.
abstract: Galactoseβ1-4Fucose (Galβ1-4Fuc) is a unique disaccharide exclusively found in N-glycans of protostomia, and is recognized by some galectins of Caenorhabditis elegans and Coprinopsis cinerea. In the present study, we investigated whether mammalian galectins also bind such a disaccharide. We examined sugar-binding ability of human galectin-1 (hGal-1) and found that hGal-1 preferentially binds Galβ1-4Fuc compared to Galβ1-4GlcNAc, which is its endogenous recognition unit. We also tested other human and mouse galectins, i.e., hGal-3, and -9 and mGal-1, 2, 3, 4, 8, and 9. All of them also showed substantial affinity to Galβ1-4Fuc disaccharide. Further, we assessed the inhibitory effect of Galβ1-4Fuc, Galβ1-4Glc, and Gal on the interaction between hGal-1 and its model ligand glycan, and found that Galβ1-4Fuc is the most effective. Although the biological significance of galectin-Galβ1-4Fuc interaction is obscure, it might be possible that Galβ1-4Fuc disaccharide is recognized as a non-self-glycan antigen. Furthermore, Galβ1-4Fuc could be a promising seed compound for the synthesis of novel galectin inhibitors.
authors.affiliation: Laboratory of Molecular Immunology, Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan. t-take@josai.ac.jp
authors.forename: Tomoharu
authors.initials: T
authors.lastname: Takeuchi
authors.forename: Mayumi
authors.initials: M
authors.lastname: Tamura
authors.forename: Kazusa
authors.initials: K
authors.lastname: Nishiyama
authors.forename: Jun
authors.initials: J
authors.lastname: Iwaki
authors.forename: Jun
authors.initials: J
authors.lastname: Hirabayashi
authors.forename: Hideyo
authors.initials: H
authors.lastname: Takahashi
authors.forename: Hideaki
authors.initials: H
authors.lastname: Natsugari
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Arata
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Kasai
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity; D004187:Disaccharides / Q000378:metabolism*; D037483:Galectin 1 / Q000378:metabolism*; D037502:Galectin 3 / Q000378:metabolism; D006023:Glycoproteins / Q000378:metabolism*; D006801:Humans; D007785:Lactose / Q000378:metabolism; D008024:Ligands; D051379:Mice; D008968:Molecular Conformation; D011485:Protein Binding; D025941:Protein Interaction Mapping; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: D004187:Disaccharides; D037483:Galectin 1; D037502:Galectin 3; D006023:Glycoproteins; C445499:LGALS1 protein, human; C485095:Lgals3 protein, mouse; D008024:Ligands; D011994:Recombinant Proteins; C547518:galactopyranosyl-1-4-fucose; D007785:Lactose
keywords: CRD; Frontal affinity chromatography; Fuc; Gal; Galectin; Galβ1-4Fuc; Galβ1-4GlcNAc; Glc; Inhibitor; carbohydrate-recognition domain; fucose; galactose; glucose