title: Determination of fructose 1,6-bisphosphate using a double-receptor sandwich type fluorescence sensing method based on uranyl-salophen complexes.
abstract: In this paper, we report a double-receptor sandwich type fluorescence sensing method for the determination of fructose bisphosphates (FBPs) using fructose 1,6-bisphosphate (F-1,6-BP) as a model analyte based on uranyl-salophen complexes. The solid phase receptor is an immobilized uranyl-salophen (IUS) complex which is bound on the surface of glass slides by covalent bonds. The labeled receptor is another uranyl-salophen complex containing a fluorescence group, or uranyl-salophen-fluorescein (USF). In the procedure of determining F-1,6-BP in sample solution, F-1,6-BP is first adsorbed on the surface of the glass slide through the coordination reaction of F-1,6-BP with IUS. It then binds USF through another coordination reaction to form a sandwich-type structure of IUS-F-1,6-BP-USF. The amount of F-1,6-BP is detected by the determination of the fluorescence intensity of IUS-F-1,6-BP-USF bound on the glass slide. Under optimal conditions, the linear range for the detection of F-1,6-BP is 0.05-5.0 nmol mL(-1) with a detection limit of 0.027 nmol mL(-1). The proposed method has been successfully applied for the determination of F-1,6-BP in real samples with satisfactory results.
authors.affiliation: College of Chemistry and Chemical Engineering, University of South China, Hengyang, Hunan 421001, China.
authors.forename: Guangliang
authors.initials: G
authors.lastname: Zhang
authors.forename: Lifu
authors.initials: L
authors.lastname: Liao
authors.forename: Yingwu
authors.initials: Y
authors.lastname: Lin
authors.forename: Miao
authors.initials: M
authors.lastname: Yang
authors.forename: Xilin
authors.initials: X
authors.lastname: Xiao
authors.forename: Changming
authors.initials: C
authors.lastname: Nie
mesh_terms: D001665:Binding Sites; D015374:Biosensing Techniques / Q000379:methods*; D002138:Calibration; D005456:Fluorescent Dyes / Q000737:chemistry; D005635:Fructosediphosphates / Q000032:analysis*; D005898:Glass; D008163:Luminescent Measurements / Q000379:methods*; D015394:Molecular Structure; D009942:Organometallic Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D012015:Reference Standards; D012680:Sensitivity and Specificity; D060327:Solid-Phase Synthesis Techniques
chemical_list: D005456:Fluorescent Dyes; D005635:Fructosediphosphates; D009942:Organometallic Compounds; C518147:uranyl salophen; C029063:fructose-1,6-diphosphate