title: Evaluation of the osteogenic activity of the BMP-2 mimetic peptide, PEP7, in vitro and in vivo.
abstract: OBJECTIVE
  To evaluate the osteogenic activity of a novel synthetic peptide, PEP7, derived from bone morphogenetic protein 2 (BMP-2) on MG-63, a human osteoblast-like cell line, and on new bone formation in vivo.
  
  
  METHODS
  The novel synthetic peptide was synthesized by a standard Fmoc method and purified to 98% purity. Cell adhesion, proliferation, and differentiation of MG-63 were observed in the presence of different concentrations of PEP7. Eight micropigs were used to evaluate new bone formation in a supra-alveolar peri-implant defect model. The PEP7-coated implants were randomly allocated to mandible defect sites. The animals were sacrificed after 8 weeks for histologic analysis.
  
  
  RESULTS
  PEP7 affected an early stage of adhesion and dose-dependently stimulated differentiation of MG-63 cells. The cell adhesion rate in the group coated with 1 μM PEP7 increased approximately 47% compared to the uncoated group and 32% compared to the group coated with recombinant human bone morphogenetic protein 2 (rhBMP-2) (P < .05). The alkaline phosphatase activities of groups treated with 50 μM of PEP7 were higher than for the other groups. PEP7 induced production of osteoblast-specific proteins in MG-63 cells. The largest effect was caused by 50 μM PEP7, followed by the groups treated with 20 μM synthetic peptide and 10 ng/mL rhBMP-2 (P < .05).
  
  
  CONCLUSIONS
  A novel synthetic peptide derived from BMP-2 has osteoinductivity and new bone formation effects, including vertical augmentation of the alveolar ridge.
authors.affiliation: Implant R&D Center, Osstem Implant, Busan, Korea.
authors.forename: Eun-Jung
authors.initials: EJ
authors.lastname: Kang
authors.forename: Su-Kyung
authors.initials: SK
authors.lastname: Kim
authors.forename: Tae-Gwan
authors.initials: TG
authors.lastname: Eom
authors.forename: Kyoo-Ok
authors.initials: KO
authors.lastname: Choi
authors.forename: Tae-Ho
authors.initials: TH
authors.lastname: Lee
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000539:Alveolar Process / Q000187:drug effects* / Q000502:physiology; D000540:Alveolar Ridge Augmentation; D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology*; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D015921:Dental Implants; D006801:Humans; D008334:Mandible / Q000187:drug effects* / Q000201:enzymology / Q000502:physiology; D010006:Osteoblasts / Q000187:drug effects* / Q000201:enzymology / Q000502:physiology; D010012:Osteogenesis / Q000187:drug effects* / Q000502:physiology; D011897:Random Allocation; D011994:Recombinant Proteins / Q000494:pharmacology; D013552:Swine; D013556:Swine, Miniature; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
chemical_list: D055396:Bone Morphogenetic Protein 2; D015921:Dental Implants; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; D000469:Alkaline Phosphatase