title: Discovery of cryptic polyketide metabolites from dermatophytes using heterologous expression in Aspergillus nidulans.
abstract: Dermatophytes belonging to the Trichophyton and Arthroderma genera cause skin infections in humans and animals. From genome sequencing data, we mined a conserved gene cluster among dermatophytes that are homologous to one that produces an immunosuppressive polyketide in Aspergillus fumigatus. Using a recombination-based cloning strategy in yeast, we constructed fungal heterologous expression vectors that encode the cryptic clusters. When integrated into the model Aspergillus nidulans host, a structurally related compound neosartoricin B was formed, suggesting a possible role of this compound in the pathogenesis of these strains.
authors.affiliation: Department of Chemical and Biomolecular Engineering and ‡Department of Chemistry and Biochemistry, University of California , Los Angeles, California 90095, United States.
authors.forename: Wen-Bing
authors.initials: WB
authors.lastname: Yin
authors.forename: Yit Heng
authors.initials: YH
authors.lastname: Chooi
authors.forename: Adam R
authors.initials: AR
authors.lastname: Smith
authors.forename: Ralph A
authors.initials: RA
authors.lastname: Cacho
authors.forename: Youcai
authors.initials: Y
authors.lastname: Hu
authors.forename: Theodore C
authors.initials: TC
authors.lastname: White
authors.forename: Yi
authors.initials: Y
authors.lastname: Tang
mesh_terms: D003883:Arthrodermataceae / Q000737:chemistry* / Q000235:genetics; D001233:Aspergillus nidulans / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D005800:Genes, Fungal; D005810:Multigene Family; D009154:Mutation; D048630:Polyketide Synthases / Q000235:genetics / Q000378:metabolism; D061065:Polyketides / Q000378:metabolism; D064210:Secondary Metabolism
chemical_list: D005656:Fungal Proteins; D061065:Polyketides; D048630:Polyketide Synthases