title: Adult renal mesenchymal stem cell-like cells contribute to juxtaglomerular cell recruitment.
abstract: The renin-angiotensin-aldosterone system (RAAS) regulates BP and salt-volume homeostasis. Juxtaglomerular (JG) cells synthesize and release renin, which is the first and rate-limiting step in the RAAS. Intense pathologic stresses cause a dramatic increase in the number of renin-producing cells in the kidney, termed JG cell recruitment, but how this occurs is not fully understood. Here, we isolated renal CD44(+) mesenchymal stem cell (MSC)-like cells and found that they differentiated into JG-like renin-expressing cells both in vitro and in vivo. Sodium depletion and captopril led to activation and differentiation of these cells into renin-expressing cells in the adult kidney. In summary, CD44(+) MSC-like cells exist in the adult kidney and can differentiate into JG-like renin-producing cells under conditions that promote JG cell recruitment.
authors.affiliation: Mandel Center for Hypertension and Atherosclerosis Research and the Cardiovascular Research Center, Duke University Medical Center, Durham, NC 27710, USA.
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Jose A
authors.initials: JA
authors.lastname: Gomez
authors.forename: Sabine
authors.initials: S
authors.lastname: Klein
authors.forename: Zhiping
authors.initials: Z
authors.lastname: Zhang
authors.forename: Barbara
authors.initials: B
authors.lastname: Seidler
authors.forename: Yanqiang
authors.initials: Y
authors.lastname: Yang
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Schmeckpeper
authors.forename: Lunan
authors.initials: L
authors.lastname: Zhang
authors.forename: Garrett G
authors.initials: GG
authors.lastname: Muramoto
authors.forename: John
authors.initials: J
authors.lastname: Chute
authors.forename: Richard E
authors.initials: RE
authors.lastname: Pratt
authors.forename: Dieter
authors.initials: D
authors.lastname: Saur
authors.forename: Maria
authors.initials: M
authors.lastname: Mirotsou
authors.forename: Victor J
authors.initials: VJ
authors.lastname: Dzau
mesh_terms: D053687:Adult Stem Cells / Q000378:metabolism*; D000818:Animals; D002216:Captopril / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D007606:Juxtaglomerular Apparatus / Q000166:cytology* / Q000378:metabolism; D007668:Kidney / Q000166:cytology* / Q000378:metabolism; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D012083:Renin / Q000378:metabolism*; D012084:Renin-Angiotensin System / Q000187:drug effects / Q000502:physiology*
chemical_list: D002216:Captopril; D012083:Renin