title: Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling.
authors.affiliation: Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore.
authors.forename: Zhengqiu
authors.initials: Z
authors.lastname: Li
authors.forename: Piliang
authors.initials: P
authors.lastname: Hao
authors.forename: Lin
authors.initials: L
authors.lastname: Li
authors.forename: Chelsea Y J
authors.initials: CY
authors.lastname: Tan
authors.forename: Xiamin
authors.initials: X
authors.lastname: Cheng
authors.forename: Grace Y J
authors.initials: GY
authors.lastname: Chen
authors.forename: Siu Kwan
authors.initials: SK
authors.lastname: Sze
authors.forename: Han-Ming
authors.initials: HM
authors.lastname: Shen
authors.forename: Shao Q
authors.initials: SQ
authors.lastname: Yao
mesh_terms: D000480:Alkynes / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D003432:Cross-Linking Reagents; D003978:Diazomethane / Q000737:chemistry*; D015195:Drug Design; D005455:Fluorescent Antibody Technique; D056945:Hep G2 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D007700:Kinetics; D015335:Molecular Probes / Q000737:chemistry*; D015394:Molecular Structure; D019920:Photoaffinity Labels / Q000737:chemistry; D047428:Protein Kinase Inhibitors / Q000138:chemical synthesis* / Q000494:pharmacology; D011494:Protein Kinases / Q000737:chemistry* / Q000378:metabolism; D020543:Proteome / Q000032:analysis*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D000480:Alkynes; D003432:Cross-Linking Reagents; D015335:Molecular Probes; D019920:Photoaffinity Labels; D047428:Protein Kinase Inhibitors; D020543:Proteome; D003978:Diazomethane; D011494:Protein Kinases
keywords: affinity-based probes; fluorescent probes; kinases; photo-crosslinking; proteomics