title: Correlative microscopy for phylogenetic and ultrastructural characterization of microbial communities.
abstract: Transmission electron microscopy (TEM) can provide ultrastructural information for cells in microbial community samples and phylogenetic information can be recovered via molecular surveys. Here we report an approach to link these data sets by coupling fluorescence in situ hybridization (FISH) with either conventional biological or cryogenic TEM. The method could fundamentally improve our understanding of the organization and functioning of microbial communities in natural systems.
authors.affiliation: Lawrence Berkeley National Laboratory, Life Sciences Division, Berkeley, CA, USA. Department of Earth and Planetary Science Department of Environmental Science, Policy and Management, University of California, Berkeley, Berkeley, CA, USA. Centre for Microscopy and Microanalysis (CMM), The University of Queensland, St. Lucia, Queensland, Australia.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Knierim
authors.forename: Birgit
authors.initials: B
authors.lastname: Luef
authors.forename: Paul
authors.initials: P
authors.lastname: Wilmes
authors.forename: Richard I
authors.initials: RI
authors.lastname: Webb
authors.forename: Manfred
authors.initials: M
authors.lastname: Auer
authors.forename: Luis R
authors.initials: LR
authors.lastname: Comolli
authors.forename: Jillian F
authors.initials: JF
authors.lastname: Banfield