title: Simultaneous determination of 11 major components in Palmul-tang by HPLC-DAD and LC-MS-MS.
abstract: Palmul-tang, a traditional herbal medicine, is composed of eight herbs (Ginseng radix, Glycyrrhizae radix, Hoelen, Atractylodis rhizoma, Angelicae gigantis radix, Cnidii rhizoma, Paeoniae radix and Rehmanniae radix) and exhibits various bioactivities, including antiallergic and antitumor effects. In this study, an effective, reliable and accurate high-performance liquid chromatography method has been developed for the simultaneous determination of 11 marker components in Palmul-tang: hydroxymethylfurfural, albiflorin, paeoniflorin, ferulic acid, nodakenin, ginsenoside Rg1, decursinol, glycyrrhizin, 6-gingerol, ginsenoside Rg3 and decursin. All calibration curves of the 11 components indicated excellent linearity (correlation coefficient > 0.9997) within the test range. The limits of detection and quantification of each component were in the ranges of 0.08-1.03 and 0.23-3.11 µg/mL, respectively. The intra-day and inter-day relative standard deviation values were within 1.65 and 2.71%, respectively. The mean recovery values were 94.49 to 101.10%. The established method was successfully applied to the simultaneous determination of 11 major components in 12 commercial samples of Palmul-tang. The developed analytical method is simple and suitable for the quality control of Palmul-tang.
authors.affiliation: Department of Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon 200-701, Korea.
authors.forename: Bohyoung
authors.initials: B
authors.lastname: Lee
authors.affiliation: Department of Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon 200-701, Korea.
authors.forename: Jin Bae
authors.initials: JB
authors.lastname: Weon
authors.affiliation: Department of Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon 200-701, Korea.
authors.forename: Bo-Ra
authors.initials: BR
authors.lastname: Yun
authors.affiliation: Department of Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon 200-701, Korea.
authors.forename: Jiwoo
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon 200-701, Korea.
authors.forename: Min Rye
authors.initials: MR
authors.lastname: Eom
authors.affiliation: Department of Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon 200-701, Korea Research Institute of Biotechnology, Kangwon National University, Chuncheon 200-701, Korea cjma@kangwon.ac.kr.
authors.forename: Choong Je
authors.initials: CJ
authors.lastname: Ma
mesh_terms: D029969:Angelica / Q000737:chemistry; D031174:Atractylodes / Q000737:chemistry; D001952:Bridged-Ring Compounds / Q000032:analysis; D002396:Catechols / Q000032:analysis; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003373:Coumaric Acids / Q000032:analysis; D003374:Coumarins / Q000032:analysis; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005233:Fatty Alcohols / Q000032:analysis; D005662:Furaldehyde / Q000031:analogs & derivatives / Q000032:analysis; D005960:Glucosides / Q000032:analysis; D006035:Glycyrrhiza / Q000737:chemistry; D019695:Glycyrrhizic Acid / Q000032:analysis; D039821:Monoterpenes / Q000032:analysis; D005894:Panax / Q000737:chemistry; D032267:Rehmannia / Q000737:chemistry; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*; D006027:Glycosides
chemical_list: D001952:Bridged-Ring Compounds; D002396:Catechols; D003373:Coumaric Acids; D003374:Coumarins; D004365:Drugs, Chinese Herbal; D005233:Fatty Alcohols; D005662:Furaldehyde; D005960:Glucosides; D019695:Glycyrrhizic Acid; D039821:Monoterpenes; C015423:peoniflorin; D006027:Glycosides; C014959:albiflorin; C008046:5-hydroxymethylfurfural; C007845:gingerol; C004999:ferulic acid; C471579:nodakenin