title: The time of maximum post-ischemic hyperperfusion indicates infarct growth following transient experimental ischemia.
abstract: After recanalization, cerebral blood flow (CBF) can increase above baseline in cerebral ischemia. However, the significance of post-ischemic hyperperfusion for tissue recovery remains unclear. To analyze the course of post-ischemic hyperperfusion and its impact on vascular function, we used magnetic resonance imaging (MRI) with pulsed arterial spin labeling (pASL) and measured CBF quantitatively during and after a 60 minute transient middle cerebral artery occlusion (MCAO) in adult rats. We added a 5% CO2 - challenge to analyze vasoreactivity in the same animals. Results from MRI were compared to histological correlates of angiogenesis. We found that CBF in the ischemic area recovered within one day and reached values significantly above contralateral thereafter. The extent of hyperperfusion changed over time, which was related to final infarct size: early (day 1) maximal hyperperfusion was associated with smaller lesions, whereas a later (day 4) maximum indicated large lesions. Furthermore, after initial vasoparalysis within the ischemic area, vasoreactivity on day 14 was above baseline in a fraction of animals, along with a higher density of blood vessels in the ischemic border zone. These data provide further evidence that late post-ischemic hyperperfusion is a sequel of ischemic damage in regions that are likely to undergo infarction. However, it is transient and its resolution coincides with re-gaining of vascular structure and function.
authors.affiliation: Department of Neurology, University Hospital Zurich, Zurich, Switzerland. Susanne.Wegener@usz.ch
authors.forename: Susanne
authors.initials: S
authors.lastname: Wegener
authors.forename: Judith
authors.initials: J
authors.lastname: Artmann
authors.forename: Andreas R
authors.initials: AR
authors.lastname: Luft
authors.forename: Richard B
authors.initials: RB
authors.lastname: Buxton
authors.forename: Michael
authors.initials: M
authors.lastname: Weller
authors.forename: Eric C
authors.initials: EC
authors.lastname: Wong
mesh_terms: D000818:Animals; D001921:Brain / Q000098:blood supply / Q000473:pathology; D002545:Brain Ischemia / Q000175:diagnosis*; D002544:Cerebral Infarction / Q000175:diagnosis*; D002560:Cerebrovascular Circulation; D004195:Disease Models, Animal; D008279:Magnetic Resonance Imaging; D008297:Male; D051381:Rats; D015427:Reperfusion Injury / Q000175:diagnosis*; D013997:Time Factors