title: The effects of hyperbaric oxygen therapy upon ototoxic injuries produced by amikacin in guinea pigs.
abstract: Hyperbaric oxygen therapy (HBOT) has enhanced the prevention and treatment of auditory ailments such as ototoxicity.
  
  
  OBJECTIVE
  To study the effects of HBOT upon ototoxic injuries produced by amikacin.
  
  
  METHODS
  This experimental study included 12 albino guinea pigs, whose auditory function was assessed through distortion product otoacoustic emissions (DPOAEs) and brainstem auditory evoked potentials (BAEPs) before and after the administration of amikacin (600 mg/kg/day) and HBOT sessions (2 ATA, 60 minutes). Morphological features were analyzed through scanning electron microscopy. Subjects were divided into four groups, as follows: group 1 - saline solution + HBOT; group 2 - amikacin for 8 days; group 3 - amikacin + seven days of rest; and group 4 - amikacin + HBOT.
  
  
  RESULTS
  Group 1 subjects had preserved function and morphology throughout the experiment; Group 2 subjects had statistically significant levels of hair cell injury and functional impairment; Subjects on groups 3 and 4 had statistically significant functional and morphological impairment after the administration of amikacin, which were still present after the proposed procedures had been carried out.
  
  
  CONCLUSIONS
  Hyperbaric oxygen therapy did not change the cochlear hair cell morphology or the electro-physiological thresholds of the guinea pigs given amikacin.
authors.affiliation: Department of Ophthalmology, Otorhinolaryngology and Head and Neck Surgery, Ribeirão Preto Medical School-FMRP, University of São Paulo-USP, Brazil.
authors.forename: Luciana de Albuquerque Salviano
authors.initials: Lde A
authors.lastname: Amora
authors.forename: Adriana de Andrade Batista
authors.initials: Ade A
authors.lastname: Murashima
authors.forename: Maria
authors.initials: M
authors.lastname: Rossato
authors.forename: Márcia Bento
authors.initials: MB
authors.lastname: Moreira
authors.forename: Miguel Ângelo
authors.initials: MÂ
authors.lastname: Hyppolito
authors.forename: Djalma José
authors.initials: DJ
authors.lastname: Fagundes
mesh_terms: D000583:Amikacin / Q000633:toxicity*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D016057:Evoked Potentials, Auditory, Brain Stem / Q000187:drug effects*; D006168:Guinea Pigs; D006198:Hair Cells, Auditory / Q000187:drug effects* / Q000648:ultrastructure; D006931:Hyperbaric Oxygenation; D008855:Microscopy, Electron, Scanning; D017084:Otoacoustic Emissions, Spontaneous / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D000583:Amikacin