title: Nanoparticles produced by ring-opening metathesis polymerization using norbornenyl-poly(ethylene oxide) as a ligand-free generic platform for highly selective in vivo tumor targeting.
abstract: We described a norbornenyl-poly(ethylene oxide) nanoparticles ligand-free generic platform, made fluorescent with straightforward preparation by ring-opening metathesis polymerization (ROMP). Our method allowed to easily obtain a drug delivery system (DDS) with facilitated functionalization by means of azide-alkyne click chemistry and with a high selectivity for the tumor in vivo, while cellular internalization is obtained without cell targeting strategy. We demonstrated that our nanoparticles are internalized by endocytosis and colocalized with acidic intracellular compartments in two models of aggressive tumoral cell lines with low prognostic and limited therapeutic treatments. Our nanoparticles could be of real interest to limit the toxicity and to increase the clinical benefit of drugs suffering rapid clearance and side effects and an alternative for cancers with poorly efficient therapeutic solutions by associating the drug delivery in the tumor tissue with an acid-sensitive release system.
authors.affiliation: Inserm, U892, CNRS, UMR 6299, and University of Nantes, 8 Quai Moncousu, 44007 Nantes cedex 1, France.
authors.forename: Fabien
authors.initials: F
authors.lastname: Gueugnon
authors.forename: Iza
authors.initials: I
authors.lastname: Denis
authors.forename: Daniel
authors.initials: D
authors.lastname: Pouliquen
authors.forename: Floraine
authors.initials: F
authors.lastname: Collette
authors.forename: Régis
authors.initials: R
authors.lastname: Delatouche
authors.forename: Valérie
authors.initials: V
authors.lastname: Héroguez
authors.forename: Marc
authors.initials: M
authors.lastname: Grégoire
authors.forename: Philippe
authors.initials: P
authors.lastname: Bertrand
authors.forename: Christophe
authors.initials: C
authors.lastname: Blanquart
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy*; D000077192:Adenocarcinoma of Lung; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000627:therapeutic use; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D016503:Drug Delivery Systems; D004705:Endocytosis; D006801:Humans; D008175:Lung Neoplasms / Q000188:drug therapy*; D008654:Mesothelioma / Q000188:drug therapy*; D000086002:Mesothelioma, Malignant; D051379:Mice; D008819:Mice, Nude; D053758:Nanoparticles; D009368:Neoplasm Transplantation; D011092:Polyethylene Glycols / Q000737:chemistry / Q000378:metabolism*; D058105:Polymerization; D011108:Polymers
chemical_list: D000970:Antineoplastic Agents; D011108:Polymers; D011092:Polyethylene Glycols