title: Detection of Mycobacterium bovis and Mycobacterium tuberculosis from Cattle: Possible Public Health Relevance.
abstract: Mycobacterium bovis and Mycobacterium tuberculosis infect both animals and humans. The disease epidemiology by these agents differs in developed and developing countries due to the differences in the implementation of the prevention and control strategies. The present study describes the detection of M. bovis and M. tuberculosis from specimens of lungs and pulmonary lymph nodes of four cattle died in an organized herd of 183 cattle in the state of Himachal Pradesh, India, with inconclusive skin test results. Identification and distinction of these closely related mycobacterial species was done by PCR-RFLP targeting hsp65 gene followed by spacer oligonucleotide typing. Mixed infection of M. bovis and M. tuberculosis was detected in one cattle.
authors.affiliation: Department of Veterinary Microbiology, College of Veterinary and Animal Science, CSK HPKV, Palampur, Himachal Pradesh India ; National Veterinary Institute, Technical University of Denmark (DTU), Copenhagen, Denmark.
authors.forename: Aneesh
authors.initials: A
authors.lastname: Thakur
authors.forename: Mandeep
authors.initials: M
authors.lastname: Sharma
authors.forename: Vipin C
authors.initials: VC
authors.lastname: Katoch
authors.forename: Prasenjit
authors.initials: P
authors.lastname: Dhar
authors.forename: R C
authors.initials: RC
authors.lastname: Katoch
keywords: Cattle; M. bovis; M. tuberculosis; Public health; Spoligotyping