title: Rapid genotyping of genetically modified laboratory animals from whole blood samples without DNA preparation.
abstract: A new, rapid method is described which permits the genotyping of genetically modified animals from a microlitre volume of whole blood samples via one step polymerase chain reaction amplification. The major advantage of the presented method is the exclusion of a DNA preparation step, which significantly reduces the time expenditure and work load of the genetic testing. Pilot studies indicate, that this method is efficient and applicable also on tissue biopsies and larger amount of blood providing a rapid and reliable new technique over conventional genotyping approaches.
authors.affiliation: Department of Microbiology and Immunobiology, University of Szeged, Szeged, Hungary.
authors.forename: A
authors.initials: A
authors.lastname: Horváth
authors.forename: P
authors.initials: P
authors.lastname: Sántha
authors.forename: V
authors.initials: V
authors.lastname: Horváth
authors.forename: Nóra
authors.initials: N
authors.lastname: Török
authors.forename: I
authors.initials: I
authors.lastname: Nagy
authors.forename: G
authors.initials: G
authors.lastname: Jancsó
authors.forename: Cs
authors.initials: C
authors.lastname: Vágvölgyi
authors.forename: F
authors.initials: F
authors.lastname: Somogyvári
mesh_terms: D000818:Animals; D001769:Blood; D004247:DNA / Q000737:chemistry / Q000235:genetics; D060005:Genotyping Techniques / Q000379:methods*; D051379:Mice; D018345:Mice, Knockout; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004247:DNA