title: Hypoxia affects in vitro proliferation and differentiation of mouse corneal epithelial progenitor cell.
abstract: This study was to investigate the proliferation and differentiation of mouse corneal epithelial progenitor cell in hypoxic airlift culture. Mouse corneal epithelial progenitor cell line progenitor cells were cultured under airlift with normoxic and hypoxic conditions for various durations up to 2 wk. Under normoxic conditions when exposed to air, the hyperproliferation and abnormal epidermal-like differentiation of mouse corneal epithelium was induced, whereas when exposed to air under hypoxic conditions, although we observed augmented proliferation, the abnormal differentiation was inhibited. The mechanism by which hypoxia prevents abnormal differentiation may involve downregulation of Wnt signaling pathways, which were inhibited in cells cultured with hypoxic airlift technique. In conclusion, hypoxia can prevent abnormal differentiation while enhancing the proliferation of corneal epithelial cells by blocking Wnt/β-catenin signaling pathway.
authors.affiliation: Eye Institute and Affiliated Xiamen Eye Center of Xiamen University, Xiamen, Fujian 361005, China.
authors.forename: Nuo
authors.initials: N
authors.lastname: Dong
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Qin
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Xue
authors.forename: Cheng
authors.initials: C
authors.lastname: Li
authors.forename: Zuguo
authors.initials: Z
authors.lastname: Liu
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D015687:Cell Hypoxia; D049109:Cell Proliferation; D015536:Down-Regulation; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D019573:Epithelium, Corneal / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D015398:Signal Transduction / Q000235:genetics; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D051153:Wnt Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D051153:Wnt Proteins