title: Characterization of a key residue for hyperfusogenic phenotype in human parainfluenza virus type 2 (hPIV-2) fusion glycoprotein.
abstract: Human parainfluenza viruses (hPIV) are pathogens responsible for upper and lower respiratory tract infections. We previously described clinical variant strains of hPIV-2 that display unusual large syncytial cytopathic effects. Their molecular characterization revealed a recurrent conserved specific amino acid substitution: A96T in the F2 subunit of the fusion glycoprotein F. The objective of this study was to investigate the contribution of this A96T substitution to the specific hyperfusogenic properties of the hPIV-2 variant strains. Based on a transient expression strategy, quantification of cell-cell fusion assays, and flow cytometry, we have shown that the A96T mutation strongly alters the fusogenic properties of F hPIV-2, highlighting this key residue in the F2 subunit and its possible role in fusion regulation. This work highlights the benefits of monitoring genetic and phenotypic changes of circulating strains to complete our understanding of Paramyxovirus fusion and related pathogenesis.
authors.affiliation: Laboratoire de Virologie et Pathologies Humaines VirPath, EA4610 UCBL/HCL, Equipe VirCell, Faculté de Médecine Laennec, Université Claude Bernard Lyon 1, Université de Lyon, 69372, Lyon Cedex 08, France.
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Le Bayon
authors.forename: Olivier
authors.initials: O
authors.lastname: Terrier
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Cartet
authors.forename: Bruno
authors.initials: B
authors.lastname: Lina
authors.forename: Manuel
authors.initials: M
authors.lastname: Rosa-Calatrava
mesh_terms: D019943:Amino Acid Substitution; D002459:Cell Fusion; D002460:Cell Line; D004847:Epithelial Cells / Q000166:cytology / Q000821:virology; D005434:Flow Cytometry; D015870:Gene Expression; D006801:Humans; D016297:Mutagenesis, Site-Directed; D010223:Parainfluenza Virus 2, Human / Q000235:genetics / Q000502:physiology*; D014760:Viral Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D053586:Virus Internalization
chemical_list: D014760:Viral Fusion Proteins