title: Effect of selected dietary compounds on extracellular superoxide dismutase in the vitreous of chinchillas.
abstract: OBJECTIVE
  The purpose of this study was to evaluate the effect of diet on the extracellular superoxide dismutase (EC-SOD) activity in the vitreous of chinchillas.
  
  
  METHODS
  Seventy-eight male chinchillas were randomly divided into 7 subgroups: controls, receiving a standard basal diet, and 6 experimental groups additionally receiving 10% nonoxidized and 10% oxidized olive oil, a garlic extract and lipoic acid in their diet. The activities of EC-SOD were measured.
  
  
  RESULTS
  The highest EC-SOD activity was similar in the controls [2.17 ± 0.27 nitric units (NU)/mg protein] and in rabbits fed oxidized olive oil (2.29 ± 0.31 NU/mg protein; p = 0.519). Nonoxidized olive oil appeared to be the strongest antioxidant component of diet (1.58 ± 0.20 NU/mg protein) as compared with the subgroups above (p = 0.008 and p = 0.012, respectively). Administration of garlic and lipoic acid decreased the SOD activity only in the groups fed oxidized olive oil (p = 0.014).
  
  
  CONCLUSIONS
  The SOD activity in laboratory animals is possibly modulated by diet. Work will be continued to extrapolate these results to humans.
authors.affiliation: Department and Clinic of Ophthalmology, Medical University of Silesia, Katowice, Poland. wrjr@xl.wp.pl
authors.forename: W
authors.initials: W
authors.lastname: Rokicki
authors.forename: J
authors.initials: J
authors.lastname: Zalejska-Fiolka
authors.forename: E
authors.initials: E
authors.lastname: Mrukwa-Kominek
authors.forename: W
authors.initials: W
authors.lastname: Majewski
authors.forename: E
authors.initials: E
authors.lastname: Birkner
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002682:Chinchilla; D004032:Diet; D019587:Dietary Supplements; D005737:Garlic / Q000737:chemistry; D008297:Male; D000069463:Olive Oil; D018384:Oxidative Stress / Q000187:drug effects; D010938:Plant Oils / Q000494:pharmacology*; D011897:Random Allocation; D013482:Superoxide Dismutase / Q000378:metabolism*; D008063:Thioctic Acid / Q000494:pharmacology*; D014822:Vitreous Body / Q000201:enzymology*
chemical_list: D000975:Antioxidants; D000069463:Olive Oil; D010938:Plant Oils; D008063:Thioctic Acid; D013482:Superoxide Dismutase