title: Association of TLR4 Gene rs2149356 polymorphism with primary gouty arthritis in a case-control study.
abstract: BACKGROUND
  The toll-like receptor (TLR)4-interleukin1β (IL1β) signaling pathway is involved in the monosodium urate (MSU)-mediated inflammation. The aim of this present study was to determine whether the TLR4 gene rs2149356 SNP is associated with gouty arthritis (GA) susceptibility and whether rs2149356 SNP impacts the TLR4-IL1β signaling pathway molecules expression.
  
  
  RESULTS
  The rs2149356 SNP was detected in 459 GA patients and 669 control subjects (containing 459 healthy and 210 hyperuricemic subjects). Peripheral blood mononuclear cells (PBMCs) TLR4 mRNA and serum IL1β were measured in different genotype carriers, and correlations between TLR4 gene SNP and TLR4 mRNA, IL1β were investigated. The frequencies of the genotype and allele were significantly different between the GA and control groups (P<0.01, respectively). The TT genotype was associated with a significantly increased risk of GA (OR = 1.88); this finding was not influenced by making adjustments for the components of possible confounders (adjusted OR = 1.96). TLR4 mRNA and IL1β were significantly increased in the TT genotype from acute GA patients (P<0.05, respectively), and lipids were significantly different among three genotypes in the GA patients (P<0.05, respectively).
  
  
  CONCLUSIONS
  The TLR4 gene rs2149356 SNP might be associated with GA susceptibility, and might participate in regulating immune, inflammation and lipid metabolism. Further studies are required to confirm these findings.
authors.affiliation: Department of Rheumatology and Immunology of the Affiliated Hospital, North Sichuan Medical College, China.
authors.forename: Yu-Feng
authors.initials: YF
authors.lastname: Qing
authors.forename: Jing-Guo
authors.initials: JG
authors.lastname: Zhou
authors.forename: Quan-Bo
authors.initials: QB
authors.lastname: Zhang
authors.forename: Dong-Sheng
authors.initials: DS
authors.lastname: Wang
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.forename: Qi-Bin
authors.initials: QB
authors.lastname: Yang
authors.forename: Cui-Ping
authors.initials: CP
authors.lastname: Huang
authors.forename: Ling
authors.initials: L
authors.lastname: Yin
authors.forename: Shu-Yue
authors.initials: SY
authors.lastname: Pan
authors.forename: Wen-Guang
authors.initials: WG
authors.lastname: Xie
authors.forename: Meng-Yun
authors.initials: MY
authors.lastname: Zhang
authors.forename: Meng-Jun
authors.initials: MJ
authors.lastname: Pu
authors.forename: Mei
authors.initials: M
authors.lastname: Zeng
mesh_terms: D015210:Arthritis, Gouty / Q000097:blood / Q000235:genetics* / Q000473:pathology; D044466:Asian People / Q000208:ethnology; D016022:Case-Control Studies; D005006:Ethnicity / Q000235:genetics; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005916:Globulins / Q000378:metabolism; D006801:Humans; D033461:Hyperuricemia / Q000097:blood / Q000235:genetics / Q000473:pathology; D017370:Interleukin-11 / Q000097:blood / Q000378:metabolism; D053583:Interleukin-1beta / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D051197:Toll-Like Receptor 4 / Q000235:genetics* / Q000378:metabolism
chemical_list: D005916:Globulins; D017370:Interleukin-11; D053583:Interleukin-1beta; D012333:RNA, Messenger; C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4