title: Improving docosahexaenoic acid productivity of Schizochytrium sp. by a two-stage AEMR/shake mixed culture mode.
abstract: In this work, an aeration-enhanced membrane reactor (AEMR) was built to control dissolved oxygen in Schizochytrium sp. broth. The effect of culture modes, i.e. single shake and AEMR mode, on the docosahexaenoic acid (DHA) productivity of Schizochytrium sp. was investigated. Experimental results showed that the biomass production in the AEMR mode was higher than that in the single shake mode, while the final DHA productivity in single shake mode was higher than that in the AEMR mode. A two-stage mixed culture mode was proposed, in which Schizochytrium sp. was cultured in AEMR mode at a flow rate of 0.2 L min(-1) for 60 h to first increase biomass production, and then shifted to shake mode to improve DHA productivity. Compared to the single shake mode, the DHA productivity in mixed mode costed 40% less culture time and the DHA productivity at 96 h exhibited a relative increase of 60%.
authors.affiliation: Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.forename: Haiyang
authors.initials: H
authors.lastname: Zhao
authors.forename: Yaqi
authors.initials: Y
authors.lastname: Lai
authors.forename: Jianping
authors.initials: J
authors.lastname: Wu
authors.forename: Huanlin
authors.initials: H
authors.lastname: Chen
mesh_terms: D003470:Culture Media; D004281:Docosahexaenoic Acids / Q000096:biosynthesis*; D005285:Fermentation; D008962:Models, Theoretical; D058009:Stramenopiles / Q000378:metabolism*
chemical_list: D003470:Culture Media; D004281:Docosahexaenoic Acids
keywords: Dissolved oxygen; Docosahexaenoic acid; Membrane reactor; Two-stage culture