title: Kroppenstedtia guangzhouensis sp. nov., a thermoactinomycete isolated from soil.
abstract: A Gram-stain-positive, spore-forming, aerobic and filamentous thermoactinomycete, designated GD02(T), was isolated from soil in south China. The isolate could grow in the presence of 0-3.0 % NaCl (w/v), at temperatures of 30-60 °C and at pH 5.5-9.5, forming ivory-coloured colonies. When the 16S rRNA gene sequence of the isolate was compared with those of other bacteria, the highest similarity was observed with Kroppenstedtia eburnea DSM 45196(T) (96.1 % 16S rRNA gene sequence similarity). The G+C content of the genomic DNA was 56.3 mol%, the cell-wall peptidoglycan contained ll-diaminopimelic acid, the main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine and phosphatidylglycerol, and the major menaquinone was MK-7. The major cellular fatty acids (>5 %) were iso-C15 : 0, iso-C16 : 0, iso-C17 : 0 and anteiso-C15 : 0. On the basis of its phenotypic and phylogenetic properties, chemotaxonomic analysis and the results of physiological and biochemical tests, strain GD02(T) ( = CGMCC 1.12404(T) = KCTC 29149(T)) was designated the type strain of a novel species of the genus Kroppenstedtia, for which the name Kroppenstedtia guangzhouensis sp. nov. is proposed.
authors.affiliation: Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, PR China.
authors.forename: Guiqin
authors.initials: G
authors.lastname: Yang
authors.affiliation: Chemistry and Materials Institute, Sichuan Normal University, Chengdu 610068, PR China.|Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, PR China.
authors.forename: Dongxing
authors.initials: D
authors.lastname: Qin
authors.affiliation: Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, PR China.
authors.forename: Chu
authors.initials: C
authors.lastname: Wu
authors.affiliation: Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Guangdong Institute of Eco-Environmental and Soil Sciences, Guangzhou 510650, PR China.
authors.forename: Shungui
authors.initials: S
authors.lastname: Zhou
authors.affiliation: College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, PR China.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Cai
mesh_terms: D056449:Bacillales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D008969:Molecular Sequence Data; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013696:Temperature; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D003960:Diaminopimelic Acid; C062629:menaquinone 7