title: Comparison of the localization of tetrodotoxin between wild pufferfish Takifugu rubripes juveniles and hatchery-reared juveniles with tetrodotoxin administration.
abstract: To reveal the accumulation profile of tetrodotoxin (TTX) in pufferfish Takifugu rubripes juveniles, we compared the localization of TTX in various tissues among wild juveniles and hatchery-reared juveniles with or without TTX administration using immunohistochemical technique with anti-TTX monoclonal antibody. Immuno-positive reaction was observed in hepatic tissue, basal cell of skin and olfactory, olfactory epithelium, optic nerve and brain (optic tectum, cerebellum, medulla oblongata) of wild juveniles (body length: BL, 4.7-9.4 cm). TTX was detected in the same tissues as wild juveniles and epithelial cell layer of intestine of hatchery-reared juveniles (BL, 5.0-5.3 cm) to which TTX was orally administrated. No positive reaction was observed from the tissues of hatchery-reared juveniles without TTX administration. These results suggest that orally administrated TTX to the non-toxic cultured juveniles is accumulated in the same manner of wild juveniles. In addition, our study revealed that pufferfish accumulates TTX in the central nervous system.
authors.affiliation: Graduate School of Fisheries Science and Environmental Studies, Nagasaki University, 1-14 Bunkyo-Machi, Nagasaki 852-8521, Japan.
authors.forename: Kogen
authors.initials: K
authors.lastname: Okita
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Takatani
authors.forename: Junichi
authors.initials: J
authors.lastname: Nakayasu
authors.forename: Hideki
authors.initials: H
authors.lastname: Yamazaki
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Sakiyama
authors.forename: Koichi
authors.initials: K
authors.lastname: Ikeda
authors.forename: Osamu
authors.initials: O
authors.lastname: Arakawa
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Sakakura
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002490:Central Nervous System / Q000187:drug effects / Q000378:metabolism; D007150:Immunohistochemistry; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D012867:Skin / Q000187:drug effects / Q000378:metabolism; D023741:Takifugu / Q000378:metabolism*; D013779:Tetrodotoxin / Q000493:pharmacokinetics* / Q000633:toxicity; D014018:Tissue Distribution
chemical_list: D013779:Tetrodotoxin
keywords: Central nervous system; Immunohistochemistry; Pufferfish; Takifugu rublipes; Tetrodotoxin (TTX)