title: CCL3 and CXCL12 production in vitro by dental pulp fibroblasts from permanent and deciduous teeth stimulated by Porphyromonas gingivalis LPS.
abstract: OBJECTIVE
  The aim of this study was to compare the production of the chemokines CCL3 and CXCL12 by cultured dental pulp fibroblasts from permanent (PDPF) and deciduous (DDPF) teeth under stimulation by Porphyromonas gingivalis LPS (PgLPS).
  
  
  METHODS
  Primary culture of fibroblasts from permanent (n=3) and deciduous (n=2) teeth were established using an explant technique. After the fourth passage, fibroblasts were stimulated by increasing concentrations of PgLPS (0-10 µg/mL) at 1, 6 and 24 h. The cells were tested for viability through MTT assay, and production of the chemokines CCL3 and CXCL12 was determined through ELISA. Comparisons among samples were performed using One-way ANOVA for MTT assay and Two-way ANOVA for ELISA results.
  
  
  RESULTS
  Cell viability was not affected by the antigen after 24 h of stimulation. PgLPS induced the production of CCL3 by dental pulp fibroblasts at similar levels for both permanent and deciduous pulp fibroblasts. Production of CXCL12, however, was significantly higher for PDPF than DDPF at 1 and 6 h. PgLPS, in turn, downregulated the production of CXCL12 by PDPF but not by DDPF.
  
  
  CONCLUSIONS
  These data suggest that dental pulp fibroblasts from permanent and deciduous teeth may present a differential behavior under PgLPS stimulation.
authors.affiliation: Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.
authors.forename: Carla Renata
authors.initials: CR
authors.lastname: Sipert
authors.forename: Ana Carolina de Faria
authors.initials: AC
authors.lastname: Morandini
authors.forename: Karin Cristina da Silva
authors.initials: KC
authors.lastname: Modena
authors.forename: Thiago José
authors.initials: TJ
authors.lastname: Dionísio
authors.forename: Maria Aparecida Andrade Moreira
authors.initials: MA
authors.lastname: Machado
authors.forename: Sandra Helena Penha de
authors.initials: SH
authors.lastname: Oliveira
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Campanelli
authors.forename: Carlos Ferreira
authors.initials: CF
authors.lastname: Santos
mesh_terms: D000704:Analysis of Variance; D002470:Cell Survival; D002478:Cells, Cultured; D054405:Chemokine CCL3 / Q000096:biosynthesis*; D054377:Chemokine CXCL12 / Q000096:biosynthesis*; D003782:Dental Pulp / Q000166:cytology / Q000378:metabolism*; D019229:Dentition, Permanent; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D016966:Porphyromonas gingivalis / Q000378:metabolism*; D013997:Time Factors; D014094:Tooth, Deciduous / Q000378:metabolism
chemical_list: D054405:Chemokine CCL3; D054377:Chemokine CXCL12