title: A superparamagnetic Fe3O4-loaded polymeric nanocarrier for targeted delivery of evodiamine with enhanced antitumor efficacy.
abstract: The aim of this study was to design and synthesize a polymeric nanocarrier system loaded with both superparamagnetic iron oxide nanoparticles (SPIONs) and the anticancer drug evodiamine through a solvent evaporation technique. The hydrodynamic diameter of the prepared SPION-evodiamine-loaded nanocarrier was approximately 261nm, and the drug-loading content and encapsulation efficiency were 8.61±0.73% and 40.36±3.42%, respectively. The nanocarrier exhibited good superparamagnetism and an iron content of approximately 9.34%. In vitro drug release experiments showed a sustained release profile over 70h. Staining with Prussian blue confirmed that the nanocarrier could be effectively internalized into HeLa cells. MTT assays indicated that the SPION-evodiamine-loaded nanocarrier showed cytotoxicity comparable to that of free evodiamine. If an external magnetic field was applied, the SPION-loaded nanocarrier accumulated at the targeted sites and demonstrated a magnetic force-mediated targeting property with the aid of a magnetic field. Furthermore, the SPION-evodiamine-loaded nanocarrier exhibited a much higher in vivo antitumor efficacy than free evodiamine. Together, these results indicate that the SPION-evodiamine-loaded nanocarrier could effectively inhibit tumor growth both in vitro and in vivo with reduced toxicity, and therefore is a promising candidate to achieve enhanced therapeutic efficacy for clinical development.
authors.affiliation: Key laboratory for Molecular Enzymology and Engineering, the Ministry of Education, College of Life Science, Jilin University, Changchun 130012, China.
authors.forename: Yanyun
authors.initials: Y
authors.lastname: Lv
authors.forename: Guobin
authors.initials: G
authors.lastname: Ding
authors.forename: Jinghui
authors.initials: J
authors.lastname: Zhai
authors.forename: Yi
authors.initials: Y
authors.lastname: Guo
authors.forename: Guangjun
authors.initials: G
authors.lastname: Nie
authors.forename: Li
authors.initials: L
authors.lastname: Xu
mesh_terms: D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems; D004354:Drug Screening Assays, Antitumor; D006367:HeLa Cells; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry*; D010316:Particle Size; D011108:Polymers / Q000737:chemistry; D011799:Quinazolines / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D013329:Structure-Activity Relationship; D013499:Surface Properties
chemical_list: D000970:Antineoplastic Agents; D004337:Drug Carriers; D058185:Magnetite Nanoparticles; D011108:Polymers; D011799:Quinazolines; C049639:evodiamine