title: The eicosapentaenoic acid metabolite 15-deoxy-δ(12,14)-prostaglandin J3 increases adiponectin secretion by adipocytes partly via a PPARγ-dependent mechanism.
abstract: The intake of ω-3 polyunsaturated fatty acids (PUFAs), which are abundant in marine fish meat and oil, has been shown to exert many beneficial effects. The mechanisms behind those effects are numerous, including interference with the arachidonic acid cascade that produces pro-inflammatory eicosanoids, formation of novel bioactive lipid mediators, and change in the pattern of secreted adipocytokines. In our study, we show that eicosapentaenoic acid (EPA) increases secreted adiponectin from 3T3-L1 adipocytes and in plasma of mice as early as 4 days after initiation of an EPA-rich diet. Using 3T3-L1 adipocytes, we report for the first time that 15-deoxy-δ(12,14)-PGJ3 (15d-PGJ3), a product of EPA, also increases the secretion of adiponectin. We demonstrate that the increased adiponectin secretion induced by 15d-PGJ3 is partially peroxisome proliferator-activated receptor-gamma (PPAR-γ)-mediated. Finally, we show that 3T3-L1 adipocytes can synthesize 15d-PGJ3 from EPA. 15d-PGJ3 was also detected in adipose tissue from EPA-fed mice. Thus, these studies provide a novel mechanism(s) for the therapeutic benefits of ω-3 polyunsaturated fatty acids dietary supplementation.
authors.affiliation: Université de Lyon, Institut National de la Santé et de la Recherche Médicale Unité Mixte de Recherche 1060, Laboratoire de Recherche en Cardiovasculaire, Métabolisme, Diabétologie et Nutrition, Institut Multidisciplinaire de Biochimie des Lipides/Institut National des Sciences Appliquées-Lyon, Villeurbanne, France.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Lefils-Lacourtablaise
authors.forename: Mairobys
authors.initials: M
authors.lastname: Socorro
authors.forename: Alain
authors.initials: A
authors.lastname: Géloën
authors.forename: Patricia
authors.initials: P
authors.lastname: Daira
authors.forename: Cyrille
authors.initials: C
authors.lastname: Debard
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Loizon
authors.forename: Michel
authors.initials: M
authors.lastname: Guichardant
authors.forename: Zury
authors.initials: Z
authors.lastname: Dominguez
authors.forename: Hubert
authors.initials: H
authors.lastname: Vidal
authors.forename: Michel
authors.initials: M
authors.lastname: Lagarde
authors.forename: Nathalie
authors.initials: N
authors.lastname: Bernoud-Hubac
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D052242:Adiponectin / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D000273:Adipose Tissue / Q000187:drug effects / Q000378:metabolism; D000813:Anilides / Q000494:pharmacology; D000818:Animals; D004041:Dietary Fats / Q000008:administration & dosage; D015118:Eicosapentaenoic Acid / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology; D004822:Epididymis / Q000187:drug effects / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D051379:Mice; D047495:PPAR gamma / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D015230:Prostaglandin D2 / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C097240:15-deoxy-delta(12,14)-prostaglandin J2; C457499:2-chloro-5-nitrobenzanilide; D052242:Adiponectin; D000813:Anilides; D004041:Dietary Fats; D047495:PPAR gamma; D015118:Eicosapentaenoic Acid; D015230:Prostaglandin D2