title: Conjugation, characterization and toxicity of lipophosphoglycan-polyacrylic acid conjugate for vaccination against leishmaniasis.
abstract: Research on the conjugates of synthetic polyelectrolytes with antigenic molecules, such as proteins, peptides, or carbohydrates, is an attractive area due to their highly immunogenic character in comparison to classical adjuvants. For example, polyacrylic acid (PAA) is a weak polyelectrolyte and has been used in several biomedical applications such as immunological studies, drug delivery, and enzyme immobilization. However, to our knowledge, there are no studies that document immune-stimulant properties of PAA in Leishmania infection. Therefore, we aimed to develop a potential vaccine candidate against leishmaniasis by covalently conjugating PAA with an immunologically vital molecule of lipophosphoglycan (LPG) found in Leishmania parasites. In the study, LPG and PAA were conjugated by a multi-step procedure, and final products were analyzed with GPC and MALDI-TOF MS techniques. In cytotoxicity experiments, LPG-PAA conjugates did not indicate toxic effects on L929 and J774 murine macrophage cells. We assume that LPG-PAA conjugate can be a potential vaccine candidate, and will be immunologically characterized in further studies to prove its potential.
authors.affiliation: Department of Bioengineering, Yildiz Technical University, Istanbul, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Topuzogullari
authors.forename: Rabia
authors.initials: R
authors.lastname: Cakir Koc
authors.forename: Sevil
authors.initials: S
authors.lastname: Dincer Isoglu
authors.forename: Melahat
authors.initials: M
authors.lastname: Bagirova
authors.forename: Zeynep
authors.initials: Z
authors.lastname: Akdeste
authors.forename: Serhat
authors.initials: S
authors.lastname: Elcicek
authors.forename: Olga N
authors.initials: ON
authors.lastname: Oztel
authors.forename: Serap
authors.initials: S
authors.lastname: Yesilkir Baydar
authors.forename: Sezen
authors.initials: S
authors.lastname: Canim Ates
authors.forename: Adil M
authors.initials: AM
authors.lastname: Allahverdiyev
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D002460:Cell Line; D006028:Glycosphingolipids / Q000737:chemistry* / Q000633:toxicity; D007896:Leishmaniasis / Q000517:prevention & control*; D054332:Leishmaniasis Vaccines / Q000737:chemistry* / Q000633:toxicity; D051379:Mice; D014611:Vaccination; D018074:Vaccines, Conjugate / Q000737:chemistry / Q000633:toxicity
chemical_list: D000180:Acrylic Resins; D006028:Glycosphingolipids; D054332:Leishmaniasis Vaccines; D018074:Vaccines, Conjugate; C008290:lipophosphonoglycan; C006903:carbopol 940