title: siRNA-mediated knockdown of SMC1A expression suppresses the proliferation of glioblastoma cells.
abstract: SMC1A is a member of cohesin complex which has essential functions in cell cycle progression and DNA repair. Therefore, we choose SMC1A as a target gene therapy of glioblastoma. It is well known that glioblastoma has very low survival rate because of ineffectiveness of conventional treatments. This study was designed to explore the possibilities of small interfering RNA (siRNA)-mediated SMC1A silencing as alternative method of treatment. We found that the lentivirus-mediated RNAi system efficiently decreased the expression level of SMC1A. Inhibiting SMC1A expression efficiently (P < 0.001) resulted in inhibiting the proliferation and colony formation of U251 and U87MG cells. Moreover, we found that SMC1A silencing led to S cell-cycle arresting. Collectively, these results demonstrated the possibility of siRNA-mediated silencing of SMC1A as a therapeutic tool for the treatment of glioblastoma.
authors.affiliation: Department of Neurosurgery, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Wangfujing, Dongcheng District, Beijing, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.forename: Zhenxing
authors.initials: Z
authors.lastname: Zhang
authors.forename: Renzhi
authors.initials: R
authors.lastname: Wang
authors.forename: Wenbin
authors.initials: W
authors.lastname: Ma
authors.forename: Junji
authors.initials: J
authors.lastname: Wei
authors.forename: Guilin
authors.initials: G
authors.lastname: Li
mesh_terms: D001932:Brain Neoplasms / Q000235:genetics / Q000473:pathology*; D002453:Cell Cycle / Q000235:genetics; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D020868:Gene Silencing; D005909:Glioblastoma / Q000235:genetics / Q000473:pathology*; D006801:Humans; D016086:Lentivirus / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism*; D014410:Tumor Stem Cell Assay; D000098550:Structural Maintenance of Chromosome Protein 1
chemical_list: D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D000098550:Structural Maintenance of Chromosome Protein 1