title: [Effect of platelet-rich plasma on cell proliferation and osteogenic activity of pulp stem cells].
abstract: OBJECTIVE
  To evaluate the effects of different concentrations of platelet-rich plasma (PRP) on human dental pulp stem cells (hDPSC) proliferation and osteogenic differentiation activity so as to provide basis for future application of dental pulp stem cells and PRP in tissue engineering and bone repair therapy.
  
  
  METHODS
  hDPSC were isolated and cultivated in vitro. Flow cytometric analysis was carried out to test the expression of STRO-1.hDPSC were cultured in various concentrations of PRP (1%, 5%, 10%, 20%). At the 2nd and 6th day 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was tested. Osteogenic differentiation of hDPSC was assessed using alkaline phosphatase (ALP) staining and Alizarin Red staining at the 7th and 14th day.
  
  
  RESULTS
  Flow cytometric analysis demonstrated that 14.82% of hDPSC were STRO-1 positive. One percent to 20% PRP showed significant effect of promoting hDPSC proliferation. One percent to 10% PRP showed significant effect of promoting hDPSC osteogenic differentiation.
  
  
  CONCLUSIONS
  Certain concentrations of PRP can promote hDPSC proliferate and osteogenic differentiate, and this finding suggests future application of dental pulp stem cells and PRP in bone tissue engineering.
authors.affiliation: Department of Prosthodontics, Capital Medical University School of Stomatology, Beijing, China.
authors.forename: Sheng-nan
authors.initials: SN
authors.lastname: Zhao
authors.forename: Wen-fei
authors.initials: WF
authors.lastname: Liu
authors.forename: Zhen-ting
authors.initials: ZT
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D002648:Child; D003782:Dental Pulp / Q000166:cytology; D006801:Humans; D008297:Male; D008875:Middle Aged; D010012:Osteogenesis; D053657:Platelet-Rich Plasma; D013234:Stem Cells / Q000166:cytology*; D055815:Young Adult