title: Burkholderia pseudomallei infection induces the expression of apoptosis-related genes and proteins in mouse macrophages.
abstract: OBJECTIVE
  In this study, we addressed whether the production of apoptosis-related genes and proteins is induced in mouse macrophages infected with Burkholderia pseudomallei cells.
  
  
  METHODS
  Mouse macrophages were infected with B. pseudomallei cells at 0.5 hours, 1 hour, 2 hours, 4 hours, and 6 hours, respectively, followed by real-time polymerase chain reaction (PCR) array analysis. The amount of apoptosis-related proteins (caspase-3, caspase -8, caspase -9, Bax, and Bcl-2) was confirmed by Western blot.
  
  
  RESULTS
  After infection, an increase of these proteins was observed. The expression levels of other apoptosis-related genes were also determined by PCR array. Experimental results revealed that the messenger RNA levels of tumor necrosis factor ligand (e.g., tnfsf10 and tnfrs10b) and fas were increased, whereas the expression levels of some antiapoptosis genes such as Birc5, Hells, and Bnip3 were decreased.
  
  
  CONCLUSIONS
  Our study results demonstrate that the apoptosis-related genes and proteins in mouse macrophages were modulated by B. pseudomallei.
authors.affiliation: Department of Cosmeceutics, China Medical University, Taichung, Taiwan.
authors.forename: You-Cheng
authors.initials: YC
authors.lastname: Hseu
authors.affiliation: Department of Biotechnology, National Kaohsiung Normal University, Kaohsiung, Taiwan.
authors.forename: Jia-Chuen
authors.initials: JC
authors.lastname: Sung
authors.affiliation: Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung, Taiwan.
authors.forename: Bao-Sen
authors.initials: BS
authors.lastname: Shieh
authors.affiliation: Department of Life Science, National Central University, Chung-Li City, Taoyuan County, Taiwan. Electronic address: osycchna@ksts.seed.net.tw.
authors.forename: Ssu Ching
authors.initials: SC
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D016957:Burkholderia pseudomallei / Q000472:pathogenicity*; D002478:Cells, Cultured; D020869:Gene Expression Profiling; D054884:Host-Pathogen Interactions; D008264:Macrophages / Q000382:microbiology* / Q000502:physiology*; D051379:Mice; D060888:Real-Time Polymerase Chain Reaction
keywords: Apoptosis-based polymerase chain reaction array; Burkholderia pseudomallei; Caspases