title: Capturing protein-coding genes across highly divergent species.
abstract: DNA hybridization capture combined with next generation sequencing can be used to determine the sequences of hundreds of target genes across hundreds of individuals in a single experiment. However, the approach has thus far only been successfully applied to capture targets that are highly similar in sequence to the bait molecules. Here we introduce modifications that extend the reach of the method to allow efficient capture of highly divergent homologous target sequences using a single set of baits. These modifications have important implications for comparative biology.
authors.affiliation: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai Ocean University, Ministry of Education, Shanghai, China.
authors.forename: Chenhong
authors.initials: C
authors.lastname: Li
authors.forename: Michael
authors.initials: M
authors.lastname: Hofreiter
authors.forename: Nicolas
authors.initials: N
authors.lastname: Straube
authors.forename: Shannon
authors.initials: S
authors.lastname: Corrigan
authors.forename: Gavin J P
authors.initials: GJ
authors.lastname: Naylor
mesh_terms: D000818:Animals; D001710:Biotin; D004247:DNA / Q000032:analysis / Q000737:chemistry / Q000235:genetics; D015342:DNA Probes; D005796:Genes; D016678:Genome; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods*; D010802:Phylogeny; D011506:Proteins / Q000235:genetics*; D012313:RNA; D017422:Sequence Analysis, DNA / Q000379:methods*; D013045:Species Specificity
chemical_list: D015342:DNA Probes; D011506:Proteins; D012313:RNA; D001710:Biotin; D004247:DNA