title: Microwave heating for the rapid generation of glycosylhydrazides.
abstract: Conditions for simple derivatization of reducing carbohydrates via adipic acid dihydrazide microwave-assisted condensation are described. We demonstrate with a diverse set of oligo- and polysaccharides how to improve a restrictive and labor intensive conventional conjugation protocol by using microwave-assisted chemistry. We show that 5 min of microwave heating in basic or acidic conditions are adequate to generate, in increased yields, intact and functional glycosylhydrazides, whereas hours to days and acidic conditions are generally required under conventional methods.
authors.affiliation: CREAB, INAC CEA Grenoble, UMR SPrAM 5819 (CEA, CNRS, UJF), 17 rue des Martyrs, 38054 Grenoble Cedex 9, France.
authors.forename: F
authors.initials: F
authors.lastname: Mallevre
authors.forename: A
authors.initials: A
authors.lastname: Roget
authors.forename: T
authors.initials: T
authors.lastname: Minon
authors.forename: Y
authors.initials: Y
authors.lastname: Kervella
authors.forename: D
authors.initials: D
authors.lastname: Ropartz
authors.forename: M C
authors.initials: MC
authors.lastname: Ralet
authors.forename: H
authors.initials: H
authors.lastname: Canut
authors.forename: T
authors.initials: T
authors.lastname: Livache
mesh_terms: D002240:Carbohydrate Sequence; D002241:Carbohydrates / Q000138:chemical synthesis* / Q000737:chemistry; D008872:Microwaves; D020349:Surface Plasmon Resonance
chemical_list: D002241:Carbohydrates