title: Osmotic stress transcription factor 1b (Ostf1b) promotes migration properties with the modulation of epithelial mesenchymal transition (EMT) phenotype in human embryonic kidney cell.
abstract: Osmoregulation is an essential mechanism for euryhaline fish. Gill cells undergo rapid mechanism to maintain the cellular homeostasis during osmotic stress. Reports have suggested that gill cells may be able to migrate between primary filament and secondary lamella during seawater acclimination. However, the factor that can trigger such process is not well-known. Previously, we identified the osmotic stress transcription factor 1b (Ostf1b) in medaka and found that it is an early hypertonic responsive gene and can activate the c-Jun N-terminal kinase (JNK) pathway. In this report, we aim to know if Ostf1b plays the role in the migration. Ostf1b was ectopic expressed in the human embryonic kidney cell line (HEK293) to understand the Ostf1b function. Results clearly demonstrated that Ostf1b could constitutively activate the Rho kinase 1 (ROCK1) and myosin light chain 2 (MLC2) signalling pathway that promotes cell migration, epithelial mesenchymal transition (EMT) and cytoskeletal dynamics through stress fibre formation. The study supports the notion of cell migration and cytoskeleton rearrangement theories in osmoregulation.
authors.affiliation: Department of Biology, Hong Kong Baptist University, Hong Kong.
authors.forename: K P
authors.initials: KP
authors.lastname: Lai
authors.forename: Alice Y S
authors.initials: AY
authors.lastname: Law
authors.forename: Marco C C
authors.initials: MC
authors.lastname: Lau
authors.forename: Y
authors.initials: Y
authors.lastname: Takei
authors.forename: William K F
authors.initials: WK
authors.lastname: Tse
authors.forename: Chris K C
authors.initials: CK
authors.lastname: Wong
mesh_terms: D024722:Cardiac Myosins / Q000378:metabolism; D002465:Cell Movement; D058750:Epithelial-Mesenchymal Transition; D005786:Gene Expression Regulation; D057809:HEK293 Cells; D006801:Humans; D018994:Myosin Light Chains / Q000378:metabolism; D010641:Phenotype; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics; D022502:Stress Fibers / Q000378:metabolism; D062725:Tight Junction Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism*; D054460:rho-Associated Kinases / Q000378:metabolism; D020742:rhoA GTP-Binding Protein / Q000378:metabolism
chemical_list: D018994:Myosin Light Chains; D012333:RNA, Messenger; D062725:Tight Junction Proteins; D014157:Transcription Factors; C107184:myosin light chain 2; C586287:osmotic stress transcription factor 1b, human; D054460:rho-Associated Kinases; D024722:Cardiac Myosins; D020742:rhoA GTP-Binding Protein
keywords: Actin fibre; Cell migration; Epithelial mesenchymal transition, Osmotic stress transcription factor 1b, Tight junction