title: Flavipin in Chaetomium globosum CDW7, an endophytic fungus from Ginkgo biloba, contributes to antioxidant activity.
abstract: 1,2-Benzenedicarboxaldehyde-3,4,5-trihydroxy-6-methyl (flavipin) was found to be antagonistic against nematodes and fungi. Here we demonstrated that flavipin is a potent antioxidant in vitro and in vivo, which has great potential in the therapy for free radical-associated diseases. Therefore, flavipin-producing bio-source was screened from 80 endophytes in Ginkgo biloba. Seven endophytic fungi were able to synthesize antioxidant substances and identified by ITS rDNA sequences. Among them, Chaetomium globosum CDW7 was a remarkable producer of flavipin. The fermentation parameters of CDW7 were then optimized for high flavipin production. Cultured under the optimal condition (25 °C, 100/250 mL flask, 12 discs/flask, 150 rpm, pH 6.5) for 14 days, CDW7 was able to synthesize flavipin at a production of 315.5 mg/L. In addition, flavipin output was positively correlated to antioxidant activities of crude extracts with a correlation coefficient of 0.8235, indicating that flavipin was the major antioxidant component of CDW7's metabolites. These data demonstrated that CDW7 was a highly yielded bio-source of antioxidant flavipin.
authors.affiliation: College of Plant Protection, Jiangsu Key Laboratory of Pesticide Science, Nanjing Agricultural University, Nanjing 210095, People's Republic of China. yeyh@njau.edu.cn
authors.forename: Yonghao
authors.initials: Y
authors.lastname: Ye
authors.forename: Yu
authors.initials: Y
authors.lastname: Xiao
authors.forename: Liang
authors.initials: L
authors.lastname: Ma
authors.forename: Hongxia
authors.initials: H
authors.lastname: Li
authors.forename: Zhenglu
authors.initials: Z
authors.lastname: Xie
authors.forename: Minghua
authors.initials: M
authors.lastname: Wang
authors.forename: Haitian
authors.initials: H
authors.lastname: Ma
authors.forename: Huaiwu
authors.initials: H
authors.lastname: Tang
authors.forename: Junyan
authors.initials: J
authors.lastname: Liu
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D002597:Chaetomium / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D060026:Endophytes / Q000145:classification / Q000302:isolation & purification / Q000378:metabolism*; D016166:Free Radical Scavengers / Q000378:metabolism*; D020441:Ginkgo biloba / Q000382:microbiology*; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA; D009764:o-Phthalaldehyde / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: D000975:Antioxidants; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D016166:Free Radical Scavengers; C000621062:flavipin; D009764:o-Phthalaldehyde