title: Sulfated Astragalus polysaccharide can regulate the inflammatory reaction induced by LPS in Caco2 cells.
abstract: This study evaluates the effect of sulfated Astragalus polysaccharide (SAPS) on inflammatory reaction induced by LPS in Caco2 cells. Sulfated modification was conducted using the chlorosulfonic acid-pyridine method. Caco2 cells were cultured with 25, 50 and 100 μg/mL SAPS or 100 μg/mL Astragalus polysaccharide (APS) for 24 h. Then, 1 μg/mL LPS was added for the next 24 h to trigger an inflammatory response. DMEM culture medium was used as a blank control. In present study, LPS stimulation significantly increased the mRNA expression of TNF-α, IL-1β, IL-8 and TLR4, and reduced the expression of ZO-1 and occludin. Compared with the LPS control group, APS (100 μg/mL) or SAPS (100 μg/mL) administration decreased the expression of TNF-α, IL-1β and IL-8. Moreover, 25 μg/mL and 50 μg/mL SAPS down-regulated TNF-α and IL-1β expression. APS administration (100 μg/mL) up-regulated occludin expression, but did not affect ZO-1 expression. However, the expression of ZO-1 and occludin was up-regulated by lower dose SAPS administration (25 μg/mL and 50 μg/mL). Compared with the other groups, the expression of TLR4 was lower in the SAPS group at all concentrations of SAPS. These results suggest that SAPS was to be a more effective anti-inflammatory agent than APS in vitro.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Wang
authors.forename: Siyu
authors.initials: S
authors.lastname: Wang
authors.forename: Yulong
authors.initials: Y
authors.lastname: Li
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Yang
authors.forename: Junhu
authors.initials: J
authors.lastname: Yao
mesh_terms: D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D027883:Astragalus Plant / Q000737:chemistry*; D018938:Caco-2 Cells; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007249:Inflammation / Q000139:chemically induced* / Q000188:drug therapy / Q000235:genetics; D008070:Lipopolysaccharides / Q000009:adverse effects*; D062793:Occludin / Q000235:genetics; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D051197:Toll-Like Receptor 4 / Q000235:genetics; D062826:Zonula Occludens-1 Protein / Q000235:genetics
chemical_list: D000893:Anti-Inflammatory Agents; D008070:Lipopolysaccharides; D062793:Occludin; D011134:Polysaccharides; D051197:Toll-Like Receptor 4; D062826:Zonula Occludens-1 Protein
keywords: Inflammatory response; Lipopolysaccharide; Sulfated Astragalus polysaccharide