title: Natronobacterium texcoconense sp. nov., a haloalkaliphilic archaeon isolated from soil of a former lake.
abstract: A novel haloalkaliphilic archaeon, strain B23(T) was isolated from the former lake Texcoco in Mexico. The strain was Gram-stain-negative, the cells coccoid to ovoid rods, red pigmented and aerobic. Strain B23(T) grew in 1.7-4.3 M NaCl, at pH 6.5-9.5 and at 25-45 °C with optimal growth at 2.6-3.4 M NaCl, pH 7.5-8.5 and 37 °C. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain B23(T) was most closely related to Natronobacterium gregoryi SP2(T) with 97.3 % sequence similarity. The polar lipids of strain B23(T) were phosphatidylglycerol and several unidentified phospholipids. The G+C content of the DNA of the strain was 62.5 mol%. Levels of DNA-DNA relatedness between strain B23(T) and Natronobacterium gregoryi DSM 3393(T) was 32.3 %. The name Natronobacterium texcoconense sp. nov. is proposed. The type strain is B23(T) ( = CECT 8068(T) = JCM 17655(T)).
authors.affiliation: Laboratory of Soil Ecology, ABACUS, Cinvestav, Mexico.
authors.forename: Erick
authors.initials: E
authors.lastname: Ruiz-Romero
authors.affiliation: Laboratory of Soil Ecology, ABACUS, Cinvestav, Mexico.
authors.forename: Katia Berenice
authors.initials: KB
authors.lastname: Sánchez-López
authors.affiliation: Laboratory of Soil Ecology, ABACUS, Cinvestav, Mexico.
authors.forename: María de Los Angeles
authors.initials: MLA
authors.lastname: Coutiño-Coutiño
authors.affiliation: Unidad de Microscopia Electrónica, Cinvestav, Mexico.
authors.forename: Sirenia
authors.initials: S
authors.lastname: González-Pozos
authors.affiliation: Laboratory of Soil Ecology, ABACUS, Cinvestav, Mexico.
authors.forename: Juan Manuel
authors.initials: JM
authors.lastname: Bello-López
authors.affiliation: Laboratory of Soil Ecology, ABACUS, Cinvestav, Mexico.
authors.forename: María Patricia
authors.initials: MP
authors.lastname: López-Ramírez
authors.affiliation: Laboratory of Soil Ecology, ABACUS, Cinvestav, Mexico.
authors.forename: Daniel Alejandro
authors.initials: DA
authors.lastname: Ramírez-Villanueva
authors.affiliation: Laboratory of Soil Ecology, ABACUS, Cinvestav, Mexico.
authors.forename: Luc
authors.initials: L
authors.lastname: Dendooven
mesh_terms: D001482:Base Composition; D019641:DNA, Archaeal / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D060106:Lakes; D008800:Mexico; D008969:Molecular Sequence Data; D019633:Natronobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D012988:Soil Microbiology
chemical_list: D019641:DNA, Archaeal; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S