title: Pseudomonas putida KT2442 as a platform for the biosynthesis of polyhydroxyalkanoates with adjustable monomer contents and compositions.
abstract: The β-oxidation weakened Pseudomonas putida were established as a platform for the production of polyhydroxyalkanoates (PHA) with adjustable monomer compositions and micro-structures. When mutant P. putida KTOYO6ΔC (phaPCJA.c) was cultivated on mixtures of sodium butyrate and sodium hexanoate (C4:C6), random copolymers of P(3HB-co-3HHx) consisting of 3-hydroxybutyrate (3HB), 3-hydroxyhexanoate (3HHx), were accumulated with 3HHx content ranged from 19 mol% to 75 mol%. While recombinant P. putida KTQQ20 grown on mixtures of sodium hexanoate and decanoic acid (C6:C10), produced random copolymers of P(3HHx-co-3HD) consisting of 3-hydroxyhexanoate (3HHx) and 3-hydroxydecanoate (3HD), the monomer fraction of 3HHx ranged from 16 mol% to 63 mol%. The comonomer compositions were easily regulated by varying the fatty acid concentrations. P. putida KTQQ20 produced a novel diblock copolymer P3HHx-b-P(3HD-co-3HDD) consisting of 49 mol% P3HHx and 51 mol% P(3HD-co-3HDD) [35.25 mol% 3HDD (3-hydroxydodecanoate)], which was characterized by (13)C NMR, HMBC NMR, DSC, GPC and universal testing machine.
authors.affiliation: MOE Key Lab of Bioinformatics, Department of Biological Science and Biotechnology, School of Life Science, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Lakshmi
authors.initials: L
authors.lastname: Tripathi
authors.forename: Lin-Ping
authors.initials: LP
authors.lastname: Wu
authors.forename: Meng
authors.initials: M
authors.lastname: Dechuan
authors.forename: Jinchun
authors.initials: J
authors.lastname: Chen
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Chen
mesh_terms: D002152:Calorimetry, Differential Scanning; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D054813:Polyhydroxyalkanoates / Q000096:biosynthesis*; D016958:Pseudomonas putida / Q000254:growth & development / Q000378:metabolism*
chemical_list: D054813:Polyhydroxyalkanoates
keywords: Block copolymer; Polyhydroxyalkanoate; Pseudomonas putida; Random copolymer