title: Further characterization of three Yersinia enterocolitica strains with a nalidixic acid-resistant phenotype isolated from humans with diarrhea.
abstract: Antimicrobial-resistant bacteria pose a threat to public health. Three Yersinia enterocolitica strains cultured from patients presenting with diarrhea and resistant to nalidixic acid were studied. Target gene mutations in gyrA alone were identified as part of the genetic basis for this phenotype. Efflux activity was also noted, since the presence of the efflux pump inhibitor, phenylalanine-arginine-β-naphthylamide, increased susceptibility to nalidixic acid.
authors.affiliation: UCD Centre for Food Safety, School of Public Health, Physiotherapy, & Population Science, UCD Centre for Molecular Innovation & Drug Discovery, University College Dublin, Belfield, Dublin, Ireland.
authors.forename: Niall
authors.initials: N
authors.lastname: Drummond
authors.forename: Roger
authors.initials: R
authors.lastname: Stephan
authors.forename: Pippa
authors.initials: P
authors.lastname: Haughton
authors.forename: Brenda P
authors.initials: BP
authors.lastname: Murphy
authors.forename: Séamus
authors.initials: S
authors.lastname: Fanning
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D027081:DNA Gyrase / Q000235:genetics / Q000378:metabolism; D003967:Diarrhea / Q000382:microbiology*; D004151:Dipeptides / Q000378:metabolism; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D006801:Humans; D008826:Microbial Sensitivity Tests; D009154:Mutation; D009268:Nalidixic Acid / Q000494:pharmacology*; D010641:Phenotype; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D037521:Virulence Factors / Q000235:genetics; D015008:Yersinia enterocolitica / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D004151:Dipeptides; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D037521:Virulence Factors; C419365:phenylalanine arginine beta-naphthylamide; D009268:Nalidixic Acid; D027081:DNA Gyrase