title: Extension of Sphingobium sp. BHC-A to a 2,4,5-trichlorophenoxyacetic acid mineralizing strain by metabolic engineering.
abstract: The gene cassette encoding for TftAB and TftCD proteins was integrated into the 16srDNA gene of the γ-hexachlorocyclohexane (γ-HCH) mineralizing strain Sphingobium sp. BHC-A by homologous recombination. The recombinant γ-HCH mineralizing strain may degrade 2,4,5-trichlorophenoxyacetic acid at a rate of 250nmol mg [protein](-1)h(-1), and the generated intermediate 2,5-dichlorohydroquinone may be further mineralized through γ-HCH downstream degradation pathway.
authors.affiliation: Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection, Nanjing, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Ge
authors.forename: Xu
authors.initials: X
authors.lastname: Chen
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Xuewei
authors.initials: X
authors.lastname: Liao
authors.forename: Yiying
authors.initials: Y
authors.lastname: Jiao
authors.forename: Qing
authors.initials: Q
authors.lastname: Hong
authors.forename: Longjiang
authors.initials: L
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Wu
mesh_terms: D015085:2,4,5-Trichlorophenoxyacetic Acid / Q000378:metabolism*; D001673:Biodegradation, Environmental; D001556:Hexachlorocyclohexane / Q000378:metabolism; D006873:Hydroquinones / Q000378:metabolism; D060847:Metabolic Engineering / Q000379:methods*; D053858:Metabolic Networks and Pathways / Q000235:genetics; D008903:Minerals / Q000378:metabolism; D008954:Models, Biological; D042301:Sphingomonadaceae / Q000145:classification / Q000235:genetics* / Q000378:metabolism*
chemical_list: D006873:Hydroquinones; D008903:Minerals; D001556:Hexachlorocyclohexane; D015085:2,4,5-Trichlorophenoxyacetic Acid; C069879:2,5-dichlorohydroquinone
keywords: 2,4,5-Trichlorophenoxyacetic acid; 2,5-Dichlorohydroquinone; Homologous recombination; Mineralization; γ-Hexachlorocyclohexane