title: Streptococcus rubneri sp. nov., isolated from the human throat.
abstract: The novel, Gram-stain-positive, ovoid, lactic acid bacterial isolates LMG 27205, LMG 27206, LMG 27207(T) and MRI-F 18 were obtained from throat samples of healthy humans. 16S rRNA gene sequence analyses indicated that these isolates belong to the genus Streptococcus, specifically the Streptococcus mitis group, with Streptococcus australis and Streptococcus mitis as the nearest neighbours (99.45 and 98.56 % 16S rRNA gene sequence similarity to the respective type strains). Genotypic fingerprinting by fluorescent amplified fragment length polymorphism (FAFLP) and pulsed-field gel electrophoresis (PFGE), DNA-DNA hybridizations, comparative sequence analysis of pheS, rpoA and atpA and physiological and biochemical tests revealed that these bacteria formed a taxon well separated from its nearest neighbours and other species of the genus Streptococcus with validly published names and, therefore, represent a novel species, for which the name Streptococcus rubneri sp. nov. is proposed, with LMG 27207(T) ( = DSM 26920(T)) as the type strain.
authors.affiliation: Max Rubner-Institut, Federal Research Institute for Nutrition and Food, Department of Safety and Quality of Fruit and Vegetables, Haid-und-Neu-Straße 9, 76131 Karlsruhe, Germany.
authors.forename: Melanie
authors.initials: M
authors.lastname: Huch
authors.affiliation: Laboratory of Microbiology, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.
authors.forename: Katrien
authors.initials: K
authors.lastname: De Bruyne
authors.affiliation: BCCM/LMG Bacteria Collection, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.
authors.forename: Ilse
authors.initials: I
authors.lastname: Cleenwerck
authors.affiliation: Max Rubner-Institut, Federal Research Institute for Nutrition and Food, Department of Physiology and Biochemistry of Nutrition, Haid-und-Neu-Straße 9, 76131 Karlsruhe, Germany.
authors.forename: Achim
authors.initials: A
authors.lastname: Bub
authors.affiliation: Max Rubner-Institut, Federal Research Institute for Nutrition and Food, Department of Safety and Quality of Fruit and Vegetables, Haid-und-Neu-Straße 9, 76131 Karlsruhe, Germany.
authors.forename: Gyu-Sung
authors.initials: GS
authors.lastname: Cho
authors.affiliation: Max Rubner-Institut, Federal Research Institute for Nutrition and Food, Department of Physiology and Biochemistry of Nutrition, Haid-und-Neu-Straße 9, 76131 Karlsruhe, Germany.
authors.forename: Bernhard
authors.initials: B
authors.lastname: Watzl
authors.affiliation: Laboratory of Microbiology, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.
authors.forename: Isabel
authors.initials: I
authors.lastname: Snauwaert
authors.affiliation: Max Rubner-Institut, Federal Research Institute for Nutrition and Food, Department of Safety and Quality of Fruit and Vegetables, Haid-und-Neu-Straße 9, 76131 Karlsruhe, Germany.
authors.forename: Charles M A P
authors.initials: CMAP
authors.lastname: Franz
authors.affiliation: Laboratory of Microbiology, Ghent University, Ledeganckstraat 35, B-9000 Ghent, Belgium.
authors.forename: Peter
authors.initials: P
authors.lastname: Vandamme
mesh_terms: D054458:Amplified Fragment Length Polymorphism Analysis; D001482:Base Composition; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010614:Pharynx / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013291:Streptococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S