title: [Analysis of Y chromosome microdeletion in non-obstructive male infertile patients with azoospermia and severe oligozoospermia].
abstract: OBJECTIVE
  To investigate the frequency and types of the major Y chromosome microdeletions in non-obstructive infertile males with azoospermia and severe oligozoospermia in Chinese.
  
  
  METHODS
  Multiplex polymerise chain reaction (PCR) amplification was used to detect Y chromosome microdeletion in 203 nonobstructive infertile men, including 125 patients with azoospermia and 78 with severe oligozoospermia, as well as 100 fertile men in the control group.
  
  
  RESULTS
  Among the 203 non-obstructive infertile cases, 22 (10.8%) carried a Y chromosome microdeletion and the frequencies of such microdeletions were 9.6% in azoospermia and 12.8% in oligozoospermia. The most frequent microdeletions were in the AZFc, and all the severe oligospermia were found microdeletions in the AZFc. No Y chromosome microdeletion was detected in the control group.
  
  
  CONCLUSIONS
  It is necessary to offer genetic screening and counseling to infertility men prior to intracytoplasmic sperm injection (ICSI) or in-vitro fertilization (IVF).
authors.affiliation: Institute of Medical Genetics, Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu 610064, China.
authors.forename: Han
authors.initials: H
authors.lastname: Ting-Ting
authors.forename: Ding
authors.initials: D
authors.lastname: Xian-Ping
authors.forename: Wei
authors.initials: W
authors.lastname: Xia
authors.forename: Ran
authors.initials: R
authors.lastname: Jing
authors.forename: Zhang
authors.initials: Z
authors.lastname: Li-Yuan
mesh_terms: D000328:Adult; D053713:Azoospermia / Q000235:genetics*; D002872:Chromosome Deletion; D041322:Chromosomes, Human, Y / Q000235:genetics; D006801:Humans; D007248:Infertility, Male / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009845:Oligospermia / Q000235:genetics*; D012729:Sex Chromosome Aberrations; D058533:Sex Chromosome Disorders of Sex Development / Q000235:genetics*; D055815:Young Adult