title: Evaluation of acute physiological and molecular alterations in surgically developed hypothyroid Wistar rats.
abstract: OBJECTIVE
  To explore the general physiological and molecular changes occurring as a result of acute hypothyroidism.
  
  
  METHODS
  Hypothyroidism was developed by thyroidectomy in wistar rats. After surgery, animals were observed for 14 days in order to determine changes in body weight, feed consumption, rectal temperature, heart rate, and blood pressure, clinical pathological and hormonal alteration. In addition, relative changes in weight, histopathology and MHC - α and β gene expression of heart was also evaluated.
  
  
  RESULTS
  Thyroidectomised rats showed lethargy, piloerection and decreased locomotors activity. Day dependent significantly decreased body weight and feed consumption were seen in hypothyroid rats. Rectal temperature was significantly reduced at day 7 and 14 after surgery. Heart rate and blood pressure were significantly decreased at day 14 in thyroidectomized rats in comparison with euthyroid rats. Haematological parameters shown high WBC count. Serum LDL and phosphorous levels were high where as triglycerides; total protein, creatinine kinase and globulin were low. Heart weight was significantly high. Histopathology of heart tissue showed myocardial segmental degeneration. Downregulation of MHC - α and upregulation of MHC - β were seen in hypothyroid rats in comparison with euthyroid rats.
  
  
  CONCLUSIONS
  This finding suggests that deficiency of thyroid hormone (TH) in hypothyroidism is associated to a cardiac dysfunction and acute changes in body homoeostasis as result of sudden arrest of thyroid hormone.
authors.affiliation: Department of Pharmacology, A.R. College of Pharmacy, Vallabh Vidyanagar, Gujarat, India.
authors.forename: Maulik
authors.initials: M
authors.lastname: Patel
authors.forename: Vinay
authors.initials: V
authors.lastname: Mishra
authors.forename: Vaibhavi
authors.initials: V
authors.lastname: Pawar
authors.forename: Ramchandran
authors.initials: R
authors.lastname: Ranvir
authors.forename: Rajesh
authors.initials: R
authors.lastname: Sundar
authors.forename: Rajas
authors.initials: R
authors.lastname: Dabhi
keywords: Euthyroid; hypothyroidism; myosin heavy chain; thyroidectomy