title: Gold nanoparticle-oligonucleotide conjugates for the profiling of malignant melanoma phenotypes.
abstract: This chapter discusses the preparation and subsequent profiling capabilities of gold nanoparticle-oligonucleotide conjugates for multiple melanoma mRNA targets. We will outline the attachment of DNA hairpins modified with a thiol for facile attachment to gold nanoparticle surfaces through gold-sulfur bond formation. Furthermore, the ability of these conjugates to detect and distinguish phenotypic variations utilizing -several melanoma cell lines and the nonmalignant cell line, HEp-2, will be investigated using flow cytometry and RT-PCR analytical techniques. The behavior of the housekeeping probe β-actin will also be investigated as a control.
authors.affiliation: Department of Chemistry, Vanderbilt University, Nashville, TN, USA.
authors.forename: John W
authors.initials: JW
authors.lastname: Stone
authors.forename: Reese
authors.initials: R
authors.lastname: Harry
authors.forename: Owen
authors.initials: O
authors.lastname: Hendley
authors.forename: David W
authors.initials: DW
authors.lastname: Wright
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics; D002460:Cell Line; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D006046:Gold / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D055029:Inverted Repeat Sequences; D008545:Melanoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D053768:Metal Nanoparticles; D015336:Molecular Probe Techniques; D009841:Oligonucleotides / Q000235:genetics / Q000378:metabolism*; D010641:Phenotype; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor; D009841:Oligonucleotides; D006046:Gold