title: Kinetic analysis of glycogen turnover: relevance to human brain 13C-NMR spectroscopy.
abstract: A biophysical model of the glycogen molecule is developed, which takes into account the points of attack of synthase and phosphorylase at the level of the individual glucose chain. Under the sole assumption of steric effects governing enzyme accessibility to glucosyl residues, the model reproduces the known equilibrium structure of cellular glycogen at steady state. In particular, experimental data are reproduced assuming that synthase (1) operates preferentially on inner chains of the molecule and (2) exhibits a faster mobility than phosphorylase in translocating from an attacked chain to another. The model is then used to examine the turnover of outer versus inner tiers during the labeling process of isotopic enrichment (IE) experiments. Simulated data are fitted to in vivo (13)C nuclear magnetic resonance spectroscopy measurements obtained in the human brain under resting conditions. Within this experimental set-up, analysis of simulated label incorporation and retention shows that 7% to 35% of labeled glucose is lost from the rapidly turning-over surface of the glycogen molecule when stimulation onset is delayed by 7 to 11.5 hours after the end of [1-(13)C]glucose infusion as done in actual procedures. The substantial label washout before stimulation suggests that much of the subsequent activation-induced glycogenolysis could remain undetected. Overall, these results show that the molecular structure significantly affects the patterns of synthesis and degradation of glycogen, which is relevant for appropriate design of labeling experiments aiming at investigating the functional roles of this glucose reserve.
authors.affiliation: 1] MARBILab, Museo storico della fisica e Centro di studi e ricerche 'Enrico Fermi', Rome, Italy [2] Dipartimento di Fisica, Sapienza Università di Roma, Rome, Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: DiNuzzo
mesh_terms: D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002247:Carbon Isotopes; D003198:Computer Simulation; D005947:Glucose / Q000008:administration & dosage / Q000378:metabolism; D006003:Glycogen / Q000737:chemistry / Q000378:metabolism*; D024981:Glycogen Phosphorylase / Q000378:metabolism; D006006:Glycogen Synthase / Q000378:metabolism; D050261:Glycogenolysis / Q000502:physiology*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008954:Models, Biological; D008958:Models, Molecular
chemical_list: D002247:Carbon Isotopes; D006003:Glycogen; D024981:Glycogen Phosphorylase; D006006:Glycogen Synthase; D005947:Glucose