title: Apoptotic toxicity of destruxin B in human non-Hodgkin lymphoma cells.
abstract: Destruxins are fungal toxins used as insecticides. Recent reports demonstrated the potential anti-cancer activities of destruxin B (DB). This study is to discover the effects of DB in lymphoma. Flow cytometry and Western blotting were used to analyze apoptosis and protein expression, respectively, in Toledo human non-Hodgkin lymphoma cells in response to DB. Administration of DB, induced apoptosis via death receptor pathway activating Fas associated death domain (FADD), caspase 8 and caspase 3, and suppressed the cell growth. In addition, DB alterated mitochondrial membrane potential by increasing the expressions of tBid and Bax, but decreasing the levels of Bcl-2, resulting in the release of apoptosis-inducing factor (AIF). In conclusion, apoptosis of human non-Hodgkin lymphoma cells in response to DB is induced through the death receptor pathway and involves an alteration of the mitochondrial membrane potential. These findings may aid the development of novel treatment of non-Hodgkin lymphoma.
authors.affiliation: Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.
authors.forename: Pin-Zhir
authors.initials: PZ
authors.lastname: Chao
authors.forename: Yi-Ping
authors.initials: YP
authors.lastname: Chin
authors.forename: I-Uen
authors.initials: IU
authors.lastname: Hsu
authors.forename: Cheng-Ming
authors.initials: CM
authors.lastname: Liu
authors.forename: Ya-Chi
authors.initials: YC
authors.lastname: Yu
authors.forename: Ting-Kai
authors.initials: TK
authors.lastname: Leung
authors.forename: Yuarn-Jang
authors.initials: YJ
authors.lastname: Lee
authors.forename: Chien-Ho
authors.initials: CH
authors.lastname: Chen
authors.forename: Yung-Feng
authors.initials: YF
authors.lastname: Lin
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D051017:Apoptosis Regulatory Proteins / Q000378:metabolism*; D045744:Cell Line, Tumor; D047630:Depsipeptides / Q000633:toxicity*; D006801:Humans; D007306:Insecticides / Q000633:toxicity*; D008228:Lymphoma, Non-Hodgkin / Q000188:drug therapy / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects
chemical_list: D051017:Apoptosis Regulatory Proteins; D047630:Depsipeptides; D007306:Insecticides; C040424:destruxin B
keywords: 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine iodide; AIF; Apaf; Apoptosis; DB; Death receptor pathway; Destruxin B; FACS; FADD; FITC; Fas associated death domain; JC-1; Mitochondrial membrane potential; Non-Hodgkin lymphoma; PI; apoptosis-inducing factor; apoptotic protease-activating factor; destruxin B; fluorescein isothiocyanate; fluorescence-activated cell sorting; propidium iodide