title: Enantioseparation of Tröger's base derivatives by capillary electrophoresis using cyclodextrins as chiral selectors.
abstract: The enantioseparation of seven Tröger's base derivatives (TBs) was carried out by capillary electrophoresis using α-, β-, and γ-cyclodextrins as chiral selectors and phosphate at 20 mmol/l concentration, pH 2.5, as background electrolyte. The method was optimized with respect to the concentration of chosen chiral selectors (0-50 mmol/l) and the amount of organic solvent (acetonitrile, 0-25 % (v/v)) in the electrolyte. The results indicate that all the studied variables, i.e., type of chiral selector, its concentration, and the amount of the added organic solvent, have a significant impact on the enantioseparation of the studied TBs. The best results for the majority of the separated TBs were obtained utilizing β-cyclodextrin at 5 mmol/l concentration and with various amounts of acetonitrile added ranging from 5 to 15% (v/v) in the background electrolyte. For the two smallest studied TBs, γ-cyclodextrin with 10% (v/v) acetonitrile also provided good resolution.
authors.affiliation: Institute of Chemical Technology, Department of Analytical Chemistry, Technická 5, 166 28, Prague 6, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Rezanka
authors.forename: Helena
authors.initials: H
authors.lastname: Ryšavá
authors.forename: Martin
authors.initials: M
authors.lastname: Havlík
authors.forename: Milan
authors.initials: M
authors.lastname: Jakubek
authors.forename: David
authors.initials: D
authors.lastname: Sýkora
authors.forename: Vladimír
authors.initials: V
authors.lastname: Král
keywords: Tröger's base; capillary electrophoresis; chiral separation; cyclodextrins; enantioseparation