title: Up-regulation of endothelial nitric oxide synthase (eNOS), silent mating type information regulation 2 homologue 1 (SIRT1) and autophagy-related genes by repeated treatments with resveratrol in human umbilical vein endothelial cells.
abstract: Resveratrol, a polyphenolic phytoalexin found in red wine and various plants, has been reported to up-regulate the expression of endothelial NO synthase (eNOS) in human umbilical vein endothelial cells (HUVEC). However, this effect was neither long term in nature nor physiologically relevant at the concentration of resveratrol studied. In the present study, we investigated the effects of repeated treatments with a lower concentration of resveratrol on the expression of genes in HUVEC. The expression levels of eNOS and silent mating type information regulation 2 homologue 1 (SIRT1) were up-regulated in HUVEC by repeated treatments with 1 μM-resveratrol for 6 d, but not with fenofibrate. Moreover, resveratrol treatment increased the expression of autophagy-regulated genes such as γ-aminobutyric acid A receptor-associated protein (GABARAP), microtubule-associated protein 1 light chain 3B (LC3B) and autophagy-related protein 3 (ATG3), the radical scavenger activity-related metallothionein-1X (MT1X) gene and the anti-inflammatory activity-related annexin A2 (ANXA) gene. In addition, resveratrol treatment down-regulated the expression of the cell-cycle checkpoint control RAD9 homologue B (RAD9B) gene. These results indicate the beneficial effects of resveratrol on the cardiovascular system.
authors.affiliation: Department of Food Science and Nutrition, Nara Women's University, Kita-Uoya-Nishi-Machi, Nara 630-8506, Japan.
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Takizawa
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Kosuge
authors.forename: Hiroyo
authors.initials: H
authors.lastname: Awaji
authors.forename: Emi
authors.initials: E
authors.lastname: Tamura
authors.forename: Ayako
authors.initials: A
authors.lastname: Takai
authors.forename: Takaaki
authors.initials: T
authors.lastname: Yanai
authors.forename: Reiko
authors.initials: R
authors.lastname: Yamamoto
authors.forename: Koichi
authors.initials: K
authors.lastname: Kokame
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Miyata
authors.forename: Rieko
authors.initials: R
authors.lastname: Nakata
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Inoue
mesh_terms: D001343:Autophagy / Q000187:drug effects* / Q000235:genetics; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects*; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism*; D010936:Plant Extracts / Q000008:administration & dosage* / Q000494:pharmacology; D000077185:Resveratrol; D056564:Sirtuin 1 / Q000378:metabolism*; D013267:Stilbenes / Q000008:administration & dosage* / Q000494:pharmacology; D015854:Up-Regulation; D014920:Wine
chemical_list: D018797:Cell Cycle Proteins; D010936:Plant Extracts; D013267:Stilbenes; C069930:rad9 protein; D052250:Nitric Oxide Synthase Type III; C447939:SIRT1 protein, human; D056564:Sirtuin 1; D000077185:Resveratrol