title: [Impact of PAHs on the expression of PRDX in earthworm (Eisenia fetida)].
abstract: A peroxiredoxin6 (PRDX6)-like gene segment has been found in up-regulated cDNA libraries of earthworm Eisenia fetida exposed to benzo[a]pyrene(BaP). Analysis with basic local alignment search tool (BLAST) and phylogenetic analysis revealed that the gene segment contains a characteristic motif which was encoded by Cys, illustrating that this gene segment belongs to PRDX. To verify the response of PRDX in E. feitida exposed to poly aromatic hydrocarbons (PAHs), four-week pollution experiments were conducted following the methods recommended by the Organization for Economic Co-operation and Development (OECD). The results showed that the expression of PRDX was up-regulated in earthworm exposed to 1.0 mg x kg(-1) Pyrene and BaP. It was indicated that PRDX in E. fetida was the potential molecular biomarkers of antioxidative stress caused by soil contamination.
authors.affiliation: Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China. ckkang1393436@yahoo.com.cn
authors.forename: Kang-Li
authors.initials: KL
authors.lastname: Lin
authors.forename: Sen-Lin
authors.initials: SL
authors.lastname: Zheng
authors.forename: Yu-Fang
authors.initials: YF
authors.lastname: Song
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Qiu
authors.forename: Wen-Ling
authors.initials: WL
authors.lastname: Xue
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001564:Benzo(a)pyrene / Q000032:analysis / Q000633:toxicity; D004784:Environmental Monitoring / Q000379:methods*; D008969:Molecular Sequence Data; D009835:Oligochaeta / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D054465:Peroxiredoxin VI / Q000235:genetics / Q000378:metabolism*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis / Q000633:toxicity*; D012989:Soil Pollutants / Q000032:analysis / Q000633:toxicity*; D013312:Stress, Physiological / Q000502:physiology
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons; D012989:Soil Pollutants; D001564:Benzo(a)pyrene; D054465:Peroxiredoxin VI