title: Effects of imperatorin, the active component from Radix Angelicae (Baizhi), on the blood pressure and oxidative stress in 2K,1C hypertensive rats.
abstract: The 2-kidney, 1-clip (2K,1C) model of hypertension was used to investigate the potential antihypertensive and antioxidant effect of imperatorin extracted from the root of radix angelicae. After 10 weeks treatment of imperatorin, mean blood pressure (MBP) of 2K,1C hypertensive rats was obtained, and superoxide dismutase (SOD), nitric oxide (NO) and nitric oxide synthase (NOS) were measured. Malondialdehyde (MDA) and glutathione (GSH) levels, catalase (CATA), xanthine oxidase (XOD), angiotensinII (Ang II) and endothelin (ET) levels of kidney were evaluated with commercial kits. Nicotinamide adenine dinucleotidephosphate (NADPH) oxidase subunits of the renal cortial tissues were determined by RT-PCR and Western blot. 8-Iso-prostaglandin F2α (8-iso-PGF2α) of 24h urinary excretion was also measured by ELISA. MBP was significantly reduced by treatment with IMP (6.25, 12.5 and 25 mg/kg/day, i.g.) in 2K,1C hypertensive rats. Meanwhile, we found that renal CATA and XOD activities, GSH levels, plasma NO and NOS contents were significantly increased in IMP-treated groups. Plasma ET, renal Ang II levels, MDA and the 24h urinary excretion of 8-iso-PGF2α in the IMP treated group were lower than control SD group. After that, we found the mRNA expressions and protein levels of NADPH oxidase subunits in the clipped kidney were markedly reduced after IMP treated in 2K,1C hypertensive rats. IMP showed antihypertensive and antioxidant effects in the renal injury of renovascular hypertensive rats, suggesting that IMP could be of therapeutic use in preventing renal injury related hypertension.
authors.affiliation: School of Medicine, Xi'an Jiaotong University, Xi'an 710061, PR China.
authors.forename: Yan-Jun
authors.initials: YJ
authors.lastname: Cao
authors.forename: Xu
authors.initials: X
authors.lastname: He
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.forename: Lang-Chong
authors.initials: LC
authors.lastname: He
mesh_terms: D029969:Angelica / Q000737:chemistry*; D000818:Animals; D000959:Antihypertensive Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000032:analysis / Q000378:metabolism*; D001794:Blood Pressure / Q000187:drug effects; D004195:Disease Models, Animal; D011564:Furocoumarins / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D006978:Hypertension, Renovascular / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D008562:Membrane Glycoproteins / Q000187:drug effects / Q000378:metabolism; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D009569:Nitric Oxide / Q000032:analysis / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D018517:Plant Roots / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D000959:Antihypertensive Agents; D000975:Antioxidants; D011564:Furocoumarins; D008562:Membrane Glycoproteins; D009569:Nitric Oxide; C500593:Cybb protein, rat; D000074662:NADPH Oxidase 2; D019255:NADPH Oxidases; C060505:neutrophil cytosolic factor 1; C031534:imperatorin
keywords: 2K,1C hypertension; 8-Iso-PGF2α; 8-iso-prostaglandin F2α; AngII; Angelica dahurica; BP; CATA; ET; GSH; Imperatorin (IMP); MBP; MDA; NADPH; NADPH oxidase; NO; NOS; Oxidase stress; RHR; SD rats; SOD; Sprague-Dawley rats.; XOD; angiotensinII; bloodpressure; catalase; endothelin; glutathione; malondialdehyde; mean blood pressure; nicotinamide adenine dinucleotidephosphate; nitric oxide; nitric oxide synthase; renovascular-hypertensive rats, two kidneys one clip model; superoxide dismutase; xanthine oxidase