title: Efficient gamma-aminobutyric acid bioconversion by employing synthetic complex between glutamate decarboxylase and glutamate/GABA antiporter in engineered Escherichia coli.
abstract: Gamma-aminobutyric acid (GABA) is a precursor of one of the most promising heat-resistant biopolymers, Nylon-4, and can be produced by the decarboxylation of monosodium glutamate (MSG). In this study, a synthetic protein complex was applied to improve the GABA conversion in engineered Escherichia coli. Complexes were constructed by assembling a single protein-protein interaction domain SH3 to the glutamate decarboxylase (GadA and GadB) and attaching a cognate peptide ligand to the glutamate/GABA antiporter (GadC) at the N-terminus, C-terminus, and the 233rd amino acid residue. When GadA and GadC were co-overexpressed via the C-terminus complex, a GABA concentration of 5.65 g/l was obtained from 10 g/l MSG, which corresponds to a GABA yield of 93 %. A significant increase of the GABA productivity was also observed where the GABA productivity increased 2.5-fold in the early culture period due to the introduction of the synthetic protein complex. The GABA pathway efficiency and GABA productivity were enhanced by the introduction of the complex between Gad and glutamate/GABA antiporter.
authors.affiliation: School of Chemical Engineering and Bioengineering, University of Ulsan, 93 Daehakro, Nam-gu, Ulsan, 680-749, Republic of Korea.
authors.forename: Tam Dinh
authors.initials: TD
authors.lastname: Le Vo
authors.forename: Ji-seun
authors.initials: JS
authors.lastname: Ko
authors.forename: Si Jae
authors.initials: SJ
authors.lastname: Park
authors.forename: Seung Hwan
authors.initials: SH
authors.lastname: Lee
authors.forename: Soon Ho
authors.initials: SH
authors.lastname: Hong
mesh_terms: D000595:Amino Acid Sequence; D017920:Antiporters / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D060846:Cell Engineering; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D005968:Glutamate Decarboxylase / Q000235:genetics / Q000378:metabolism*; D018698:Glutamic Acid / Q000378:metabolism*; D008969:Molecular Sequence Data; D005680:gamma-Aminobutyric Acid / Q000096:biosynthesis*
chemical_list: D017920:Antiporters; D018698:Glutamic Acid; D005680:gamma-Aminobutyric Acid; D005968:Glutamate Decarboxylase