title: Evidence for two koi herpesvirus (KHV) genotypes in South Korea.
abstract: The geographic distribution of koi herpesvirus (KHV) has recently been analyzed by polymerase chain reaction (PCR, based on the alleles of 3 domains) and sequence analysis using 3 regions of KHV genomic DNA (SphI-5, 9/5, and the thymidine kinase gene). In this study, samples from 6 carp showing symptoms of KHV infection in 2008 were examined for the presence of KHV by using PCR and cell culture isolation methods. KHV was detected in 2 (Pyeongtaek and Buan) of the samples. Sequence analysis revealed that the genotype of the KHV PT-08 isolate was Asia genotype variant 1 (A1), and the genotype of the KHV BA-08 isolate was European genotype variant 4 (E4). In addition, PCR patterns and sequence analysis based on the alleles of 3 domains of an alternate KHV classification system confirmed that the genotype of the KHV PT-08 isolate was CyHV3-J, and the genotype of the KHV BA-08 isolate was CyHV3-third genotype. To our knowledge, this is the first study to demonstrate the presence of 2 genotypes of KHV (genotype A1/CyHV3-J; genotype E4/CyHV3-third genotype) in South Korea.
authors.affiliation: Animal, Plant and Fisheries Quarantine and Inspection Agency, Jungbu Regional Office, Incheon, South Korea.
authors.forename: Hyoung Jun
authors.initials: HJ
authors.lastname: Kim
authors.forename: Se Ryun
authors.initials: SR
authors.lastname: Kwon
mesh_terms: D000483:Alleles; D000818:Animals; D001483:Base Sequence; D002347:Carps; D004279:DNA, Viral / Q000235:genetics; D005393:Fish Diseases / Q000453:epidemiology / Q000821:virology*; D005838:Genotype; D006564:Herpesviridae / Q000235:genetics* / Q000302:isolation & purification; D006566:Herpesviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D008969:Molecular Sequence Data; D056910:Republic of Korea
chemical_list: D004279:DNA, Viral