title: Application of a quantitative 1H-NMR method for the determination of amygdalin in Persicae semen, Armeniacae semen, and Mume fructus.
abstract: A quantitative (1)H-NMR method (qHNMR) was used to measure the amygdalin content of Persicae semen, Armeniacae semen, and Mume fructus, in each of which amygdalin constitutes a major component. The purity of amygdalin was calculated from the ratio of the intensity of the amygdalin H-2 signal at δ 6.50 ppm in pyridine-d 5 to that of the hexamethyldisilane (HMD) signal at 0 ppm. The HMD concentration was corrected by the International System of Units (SI) traceability with certified reference material (CRM)-grade bisphenol A. qHNMR revealed the amygdalin contents to be 2.72 and 3.13% in 2 lots of Persicae semen, 3.62 and 5.19% in 2 lots of Armeniacae semen, and 0.23% in Mume fructus. Thus, we demonstrated the utility of this method for the quantitative analysis of crude drugs.
authors.affiliation: Laboratory of Pharmacognosy, College of Pharmacy, Kinjo Gakuin University, 2-1723 Omori, Moriyama-ku, Nagoya, 463-8521, Japan.
authors.forename: Rie
authors.initials: R
authors.lastname: Tanaka
authors.forename: Akane
authors.initials: A
authors.lastname: Nitta
authors.forename: Akito
authors.initials: A
authors.lastname: Nagatsu
mesh_terms: D000678:Amygdalin / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D009682:Magnetic Resonance Spectroscopy / Q000592:standards; D008517:Phytotherapy; D028321:Plant Preparations / Q000032:analysis*; D010946:Plants, Medicinal; D027861:Prunus / Q000737:chemistry*; D012015:Reference Standards
chemical_list: D028321:Plant Preparations; D000678:Amygdalin