title: The complexity of identifying cancer stem cell biomarkers.
abstract: The efficacy of glioma therapy can be considerably improved if it eliminates cancer stem cells (CSCs); however, to achieve this, CSCs markers are required. This study investigated the influence of micro-environmental changes on CSCs in hypoxic, serum deprived U87-MG and the corresponding control cells. Proteomic analysis produced a wide dataset, depicting the changes that occur at the proteomic level in the differentiated and undifferentiated U87-MG cell line. With the IPA analysis, HPRD and literature reviews, 11 proteins were proposed as potential differentiated biomarkers for CSCs namely Hsp90β1, vimentin, PGK1, GAPDH, EIF4e, TPI1, HspA8, HNRNPK, NAMPT, CCSNK2A1, and ANXA2.
authors.affiliation: Brain Tumour North West, Faculty of Science and Technology, University of Central Lancashire, Preston, UK.
authors.forename: Zarine
authors.initials: Z
authors.lastname: Khan
authors.forename: Amal
authors.initials: A
authors.lastname: Shervington
authors.forename: Chinmay
authors.initials: C
authors.lastname: Munje
authors.forename: Leroy
authors.initials: L
authors.lastname: Shervington
mesh_terms: D000071916:AC133 Antigen; D015703:Antigens, CD / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D048430:Cell Shape; D016895:Culture Media, Serum-Free; D005910:Glioma; D006023:Glycoproteins / Q000378:metabolism; D006801:Humans; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D020543:Proteome / Q000378:metabolism; D059016:Tumor Microenvironment; D015854:Up-Regulation; D014746:Vimentin / Q000378:metabolism
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D014408:Biomarkers, Tumor; D016895:Culture Media, Serum-Free; D006023:Glycoproteins; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D010455:Peptides; D020543:Proteome; D014746:Vimentin