title: Prevention of intra-abdominal adhesion by bi-layer electrospun membrane.
abstract: The aim of this study was to compare the anti-adhesion efficacy of a bi-layer electrospun fibrous membrane consisting of hyaluronic acid-loaded poly(ε-caprolactone) (PCL) fibrous membrane as the inner layer and PCL fibrous membrane as the outer layer with a single-layer PCL electrospun fibrous membrane in a rat cecum abrasion model. The rat model utilized a cecal abrasion and abdominal wall insult surgical protocol. The bi-layer and PCL membranes were applied between the cecum and the abdominal wall, respectively. Control animals did not receive any treatment. After postoperative day 14, a visual semiquantitative grading scale was used to grade the extent of adhesion. Histological analysis was performed to reveal the features of adhesion tissues. Bi-layer membrane treated animals showed significantly lower adhesion scores than control animals (p < 0.05) and a lower adhesion score compared with the PCL membrane. Histological analysis of the bi-layer membrane treated rat rarely demonstrated tissue adhesion while that of the PCL membrane treated rat and control rat showed loose and dense adhesion tissues, respectively. Bi-layer membrane can efficiently prevent adhesion formation in abdominal cavity and showed a significantly decreased adhesion tissue formation compared with the control.
authors.affiliation: Department of Orthopaedics, Shanghai Sixth People's Hospital, Shanghai Jiaotong University School of Medicine, 600 Yishan Road, Shanghai 200233, China. fancunyish@126.com.
authors.forename: Shichao
authors.initials: S
authors.lastname: Jiang
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Hede
authors.initials: H
authors.lastname: Yan
authors.forename: Cunyi
authors.initials: C
authors.lastname: Fan
mesh_terms: D000005:Abdomen / Q000473:pathology*; D000818:Animals; D002432:Cecum / Q000473:pathology; D004195:Disease Models, Animal; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D011091:Polyesters / Q000494:pharmacology; D017207:Rats, Sprague-Dawley; D013194:Staining and Labeling; D013499:Surface Properties; D000267:Tissue Adhesions / Q000517:prevention & control; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D008567:Membranes, Artificial; D011091:Polyesters; C016240:polycaprolactone