title: A biomimetic physiological model for human adipose tissue by adipocytes and endothelial cell cocultures with spatially controlled distribution.
abstract: An in vitro model that recapitulates the characteristics of native human adipose tissue would largely benefit pathology studies and therapy development. In this paper, we fabricated a physiological model composed of both human adipocytes and endothelial cells with spatially controlled distribution that biomimics the structure and composition of human adipose tissue. Detailed studies into the cell-cell interactions between the adipocytes and endothelial cells revealed a mutual-enhanced effect which resembles the in vivo routine. Furthermore, comparisons between planar coculture and model coculture demonstrated improved adipocyte function as well as endothelial cell proliferation under the same conditions. This research provided a reliable model for human adipose tissue development studies and potential obesity-related therapy development.
authors.affiliation: Department of Mechanical Engineering, Tsinghua University, Beijing 100084, People's Republic of China. yaorui@mail.tsinghua.edu.cn
authors.forename: Rui
authors.initials: R
authors.lastname: Yao
authors.forename: Yanan
authors.initials: Y
authors.lastname: Du
authors.forename: Renji
authors.initials: R
authors.lastname: Zhang
authors.forename: Feng
authors.initials: F
authors.lastname: Lin
authors.forename: Jie
authors.initials: J
authors.lastname: Luan
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D000273:Adipose Tissue / Q000378:metabolism / Q000473:pathology*; D000328:Adult; D000464:Alginates / Q000737:chemistry; D001391:Azo Compounds / Q000737:chemistry; D032701:Biomimetics / Q000379:methods*; D002448:Cell Adhesion; D002450:Cell Communication; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D018920:Coculture Techniques; D003094:Collagen / Q000737:chemistry; D042783:Endothelial Cells / Q000166:cytology*; D005260:Female; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008863:Microspheres; D009765:Obesity / Q000628:therapy; D013499:Surface Properties; D013997:Time Factors
chemical_list: D000464:Alginates; D001391:Azo Compounds; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D003094:Collagen; C011049:oil red O