title: Effects of renal pelvic high-pressure perfusion on nephrons in a porcine pyonephrosis model.
abstract: The aim of this study was to investigate the effects of various renal pelvic pressure gradients on nephrons with purulent infection. Five miniature test pigs were selected. One side of the kidney was used to prepare the pyonephrosis model and the other side was used as the healthy control. A piezometer and a water fill tube were inserted into the renal pelvis through the ureter. Prior to perfusion, punctures were made on the healthy and purulent sides of the kidneys to obtain tissues (as controls). Subsequently, a puncture biopsy was conducted on the kidneys at five pressure levels: 10, 20, 30, 40 and 50 mmHg. Once the renal pelvic pressure had increased, the healthy and injured kidneys presented pathological changes, including dilation of the renal tubule and capsule and compression of the renal glomerulus. When the renal pelvic pressure exceeded 20 mmHg, the injured kidney presented more damage. Electron microscopy revealed that the increase in pressure resulted in the following: the podocyte gap widened, the epithelial cells of the renal capsule separated from the basement membrane, the basement membrane thickness became uneven, the continuity of the basement membrane was interrupted at multiple positions and the renal tubule microvillus arrangement became disorganised. The manifestations in the pyonephrosis model were more distinct compared with those in the healthy kidney. As the renal pelvic pressure exceeds 20 mmHg under a renal purulent infection status, the nephrons become damaged. The extent of the damage is aggravated as the pressure is increased.
authors.affiliation: Department of Urology, 303rd Hospital of People's Liberation Army, Nanning, Guangxi 530021, P.R. China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: DA-Qing
authors.initials: DQ
authors.lastname: Zhou
authors.forename: Meng
authors.initials: M
authors.lastname: He
authors.forename: Wen-Gang
authors.initials: WG
authors.lastname: Li
authors.forename: Xiang
authors.initials: X
authors.lastname: Pang
authors.forename: Xiao-Xiang
authors.initials: XX
authors.lastname: Yu
authors.forename: Bo
authors.initials: B
authors.lastname: Jiang
keywords: infection; nephron; renal pelvic pressure