title: Modulatory effects of dietary inclusion of garlic (Allium sativum) on gentamycin-induced hepatotoxicity and oxidative stress in rats.
abstract: OBJECTIVE
  To investigate the ameliorative effect of dietary inclusion of garlic (Allium sativum) on gentamycin-induced hepatotoxicity in rats.
  
  
  METHODS
  Adult male rats were randomly divided into four groups with six animals in each group. Groups 1 and 2 were fed basal diet while Groups 3 and 4 were fed diets containing 2% and 4% garlic respectively for 27 d prior to gentamycin administration. Hepatotoxicity was induced by the intraperitoneal administration of gentamycin (100 mg/kg body weight) for 3 d. The liver and plasma were studied for hepatotoxicity and antioxidant indices.
  
  
  RESULTS
  Gentamycin induces hepatic damage as revealed by significant (P<0.05) elevation of liver damage marker enzymes (aspartate transaminase and alanine aminotransferase) and reduction in plasma albumin level. Gentamycin also caused a significant (P<0.05) alteration in plasma and liver enzymatic (catalase, glutathione and super oxygen dehydrogenises) and non-enzymatic (glutathione and vitamin C) antioxidant indices with concomitant increase in the malondialdehyde content; however, there was a significant (P<0.05) restoration of the antioxidant status coupled with significant (P<0.05) decrease in the tissues' malondialdehyde content, following consumption of diets containing garlic.
  
  
  CONCLUSIONS
  These results suggest that dietary inclusion of garlic powder could protect against gentamycin-induced hepatotoxicity, improve antioxidant status and modulate oxidative stress; a function attributed to their phenolic constituents.
authors.affiliation: Functional Foods, Nutraceuticals and Phytomedicine Unit, Department of Biochemistry, Federal University of Technology, Akure, P.M.B. 704 Akure, 340001, Nigeria.
authors.forename: Adedayo O
authors.initials: AO
authors.lastname: Ademiluyi
authors.forename: Ganiyu
authors.initials: G
authors.lastname: Oboh
authors.forename: Tosin R
authors.initials: TR
authors.lastname: Owoloye
authors.forename: Oluwaseun J
authors.initials: OJ
authors.lastname: Agbebi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D004032:Diet; D019587:Dietary Supplements; D005737:Garlic / Q000737:chemistry* / Q000378:metabolism*; D005839:Gentamicins / Q000633:toxicity; D008099:Liver / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats
chemical_list: D000975:Antioxidants; D005839:Gentamicins
keywords: Garlic (Allium sativum); Gentamycin; Hepatotoxicity; Oxidative stress; Phytochemicals