title: Immunolocalisation and oestrogen regulation of small proline-rich protein 2a protein in the mouse uterus.
abstract: Small proline-rich protein 2a (Sprr2a) is one of the structural components of the cornified keratinocyte cell envelope that contributes to form a protective barrier in the skin against dehydration and environmental stress. Interestingly, Sprr2a mRNA is detected in the mouse uterus and is regulated by 17β-oestradiol (E2). In the present study, we investigated the effects of E2 and oestrogenic compounds on the regulation and localisation of Sprr2a protein in the mouse uterus. Immunohistochemical staining revealed that Sprr2a protein is detected only in the adult uterus, and not in the ovary, oviduct or testis. We also demonstrated that Sprr2a protein is tightly regulated by E2 in the mouse uterus and exclusively detected in luminal and glandular epithelial cells. Furthermore, Sprr2a is dose-dependently induced by oestrogenic compounds such as bisphenol A and 4-tert-octylphenol. Collectively, our studies suggest that Sprr2a protein may have a unique function in physiological events in the mouse uterus and can be used as an indicator to detect compounds with oestrogenic activity in the mouse uterus.
authors.affiliation: Department of Obstertics and Gynecology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea.
authors.forename: Hyang-Ah
authors.initials: HA
authors.lastname: Lee
authors.affiliation: Department of Biomedical Science, CHA University, Seoul 135-081, South Korea.
authors.forename: Hye-Ryun
authors.initials: HR
authors.lastname: Kim
authors.affiliation: Center for Biomedical Engineering and Technology and Department of Anatomy and Neurobiology, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
authors.forename: Young Jin
authors.initials: YJ
authors.lastname: Lee
authors.affiliation: Department of Internal Medicine, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea.
authors.forename: Seung-Joon
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Department of Internal Medicine, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea.
authors.forename: Woo Jin
authors.initials: WJ
authors.lastname: Kim
authors.affiliation: Department of Internal Medicine, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea.
authors.forename: Seon-Sook
authors.initials: SS
authors.lastname: Han
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea.
authors.forename: Se-Ran
authors.initials: SR
authors.lastname: Yang
authors.affiliation: College of Veterinary Medicine, Kangwon National Univeristy, Chuncheon 200-701, South Korea.
authors.forename: Heung-Myong
authors.initials: HM
authors.lastname: Woo
authors.affiliation: Department of Obstertics and Gynecology, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea.
authors.forename: Sunghun
authors.initials: S
authors.lastname: Na
authors.affiliation: Department of Biomedical Science, CHA University, Seoul 135-081, South Korea.
authors.forename: Haengseok
authors.initials: H
authors.lastname: Song
authors.affiliation: Department of Internal Medicine, School of Medicine, Kangwon National University, Chuncheon 200-701, South Korea.
authors.forename: Seok-Ho
authors.initials: SH
authors.lastname: Hong
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000494:pharmacology; D055233:Cornified Envelope Proline-Rich Proteins / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism; D004958:Estradiol / Q000031:analogs & derivatives* / Q000494:pharmacology*; D004965:Estrogen Antagonists / Q000494:pharmacology*; D004968:Estrogens, Non-Steroidal / Q000494:pharmacology; D005260:Female; D000077267:Fulvestrant; D008297:Male; D051379:Mice; D010636:Phenols / Q000494:pharmacology; D013737:Testis / Q000378:metabolism; D014599:Uterus / Q000187:drug effects / Q000378:metabolism*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D055233:Cornified Envelope Proline-Rich Proteins; D004958:Estradiol; D004965:Estrogen Antagonists; D004968:Estrogens, Non-Steroidal; D000077267:Fulvestrant; D010636:Phenols; C491091:Sprr2a1 protein, mouse; C006780:bisphenol A; D000098826:Bisphenol A Compounds