title: Use of heat stress responsive gene expression levels for early selection of heat tolerant cabbage (Brassica oleracea L.).
abstract: Cabbage is a relatively robust vegetable at low temperatures. However, at high temperatures, cabbage has disadvantages, such as reduced disease tolerance and lower yields. Thus, selection of heat-tolerant cabbage is an important goal in cabbage breeding. Easier or faster selection of superior varieties of cabbage, which are tolerant to heat and disease and have improved taste and quality, can be achieved with molecular and biological methods. We compared heat-responsive gene expression between a heat-tolerant cabbage line (HTCL), "HO", and a heat-sensitive cabbage line (HSCL), "JK", by Genechip assay. Expression levels of specific heat stress-related genes were increased in response to high-temperature stress, according to Genechip assays. We performed quantitative RT-PCR (qRT-PCR) to compare expression levels of these heat stress-related genes in four HTCLs and four HSCLs. Transcript levels for heat shock protein BoHsp70 and transcription factor BoGRAS (SCL13) were more strongly expressed only in all HTCLs compared to all HSCLs, showing much lower level expressions at the young plant stage under heat stress (HS). Thus, we suggest that expression levels of these genes may be early selection markers for HTCLs in cabbage breeding. In addition, several genes that are involved in the secondary metabolite pathway were differentially regulated in HTCL and HSCL exposed to heat stress.
authors.affiliation: Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahangno, Yuseong-gu, Daejeon 305-806, Korea. secmeta2@yahoo.com.
authors.forename: Hyun Ji
authors.initials: HJ
authors.lastname: Park
authors.forename: Won Yong
authors.initials: WY
authors.lastname: Jung
authors.forename: Sang Sook
authors.initials: SS
authors.lastname: Lee
authors.forename: Jun Ho
authors.initials: JH
authors.lastname: Song
authors.forename: Suk-Yoon
authors.initials: SY
authors.lastname: Kwon
authors.forename: Hyeran
authors.initials: H
authors.lastname: Kim
authors.forename: Chulwook
authors.initials: C
authors.lastname: Kim
authors.forename: Jun Cheul
authors.initials: JC
authors.lastname: Ahn
authors.forename: Hye Sun
authors.initials: HS
authors.lastname: Cho
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D001937:Brassica / Q000235:genetics* / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D018869:Heat-Shock Response / Q000235:genetics*; D006358:Hot Temperature; D007178:Inbreeding; D055432:Metabolomics; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D019655:Quantitative Trait, Heritable; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D012621:Seasons; D064210:Secondary Metabolism / Q000235:genetics; D013312:Stress, Physiological / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins; D010940:Plant Proteins; D012333:RNA, Messenger; D014157:Transcription Factors