title: C-ANCA/proteinase 3-positive colitis in children: a distinctive form of inflammatory bowel disease or vasculitis with colitis as initial presentation?
abstract: OBJECTIVE
  Anti-neutrophil cytoplasmic antibodies (ANCAs) detected by indirect immunofluorescence have been found in patients with inflammatory bowel disease (IBD). Nevertheless, specific antibodies against proteinase-3 (PR3) are rare in this context.
  
  
  METHODS
  Sera from 30 consecutive pediatric patients with IBD were evaluated for ANCA-indirect immunofluorescence and its specific antibodies to investigate whether PR3-ANCA positivity (PR3-ANCA+) identifies a distinct IBD subtype.
  
  
  RESULTS
  The 5 PR3-ANCA+ patients (17%) showed significantly more concomitant biliary disease and severe anal blood loss (P < 0.05). None had vasculitis features at diagnosis nor during follow-up.
  
  
  CONCLUSIONS
  This pilot study demonstrates significant clinical differences between the PR3-ANCA-positive and -negative IBD subset.
authors.affiliation: *Paediatric Gastroenterology †Department of Clinical Chemistry, Microbiology and Immunology, Laboratory of Clinical Biology ‡Adult Gastroenterology, Ghent University Hospital, Belgium.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Van Biervliet
authors.forename: Carolien
authors.initials: C
authors.lastname: Bonroy
authors.forename: Saskia
authors.initials: S
authors.lastname: Vande Velde
authors.forename: Ruth
authors.initials: R
authors.lastname: De Bruyne
authors.forename: Myriam
authors.initials: M
authors.lastname: Van Winckel
authors.forename: Harald
authors.initials: H
authors.lastname: Peeters
authors.forename: Joris
authors.initials: J
authors.lastname: Delanghe
authors.forename: Veronique
authors.initials: V
authors.lastname: Stove
authors.forename: Martine
authors.initials: M
authors.lastname: De Vos
mesh_terms: D000293:Adolescent; D019268:Antibodies, Antineutrophil Cytoplasmic / Q000097:blood*; D001660:Biliary Tract Diseases / Q000209:etiology; D002648:Child; D002675:Child, Preschool; D003092:Colitis / Q000097:blood / Q000150:complications / Q000175:diagnosis* / Q000276:immunology; D003937:Diagnosis, Differential; D005260:Female; D006471:Gastrointestinal Hemorrhage / Q000209:etiology; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000097:blood / Q000150:complications / Q000175:diagnosis* / Q000276:immunology; D008297:Male; D053146:Myeloblastin / Q000276:immunology*; D020293:Vasculitis, Central Nervous System / Q000097:blood / Q000175:diagnosis* / Q000276:immunology
chemical_list: D019268:Antibodies, Antineutrophil Cytoplasmic; D053146:Myeloblastin