title: A single PLP-dependent enzyme PctV catalyzes the transformation of 3-dehydroshikimate into 3-aminobenzoate in the biosynthesis of pactamycin.
abstract: Natural amino donation: A PLP-dependent aminotransferase PctV, encoded in the pactamycin biosynthetic gene cluster, was found to catalyze the formation of 3-aminobenzoate from 3-dehydroshikimate with L-glutamate as the amino donor. The PctV reaction comprises a transamination and two dehydration reactions. This is the first report of a simple 3-ABA synthase in nature.
authors.affiliation: Department of Chemistry and Materials Science, Tokyo Institute of Technology, 2-12-1 O-okayama, Meguro-ku, Tokyo 152-8551, Japan.
authors.forename: Akane
authors.initials: A
authors.lastname: Hirayama
authors.forename: Tadashi
authors.initials: T
authors.lastname: Eguchi
authors.forename: Fumitaka
authors.initials: F
authors.lastname: Kudo
mesh_terms: D001688:Biological Products / Q000378:metabolism; D002384:Catalysis; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D010142:Pactamycin / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification; D012765:Shikimic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D013302:Streptomyces / Q000201:enzymology / Q000378:metabolism; D013379:Substrate Specificity; D062368:meta-Aminobenzoates / Q000378:metabolism*
chemical_list: D001688:Biological Products; D062368:meta-Aminobenzoates; D010142:Pactamycin; C096387:3-dehydroshikimate; D012765:Shikimic Acid; C044855:3-aminobenzoic acid
keywords: aminobenzoate; biosynthesis; dehydroshikimate; natural products; pactamycin