title: Fluorescent nanoparticle beacon for logic gate operation regulated by strand displacement.
abstract: A mechanism is developed to construct a logic system by employing DNA/gold nanoparticle (AuNP) conjugates as a basic work unit, utilizing a fluorescent beacon probe to detect output signals. To implement the logic circuit, a self-assembly DNA structure is attached onto nanoparticles to form the fluorescent beacon. Moreover, assisted by regulation of multilevel strand displacement, cascaded logic gates are achieved. The computing results are detected by methods using fluorescent signals, gel electrophoresis and transmission electron microscope (TEM). This work is expected to demonstrate the feasibility of the cascaded logic system based on fluorescent nanoparticle beacons, suggesting applications in DNA computation and biotechnology.
authors.affiliation: School of Control and Computer Engineering, North China Electric Power University, Beijing 102206, China. yangjing369@gmail.com
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Lingjing
authors.initials: L
authors.lastname: Shen
authors.forename: Jingjing
authors.initials: J
authors.lastname: Ma
authors.forename: H Inaki
authors.initials: HI
authors.lastname: Schlaberg
authors.forename: Shi
authors.initials: S
authors.lastname: Liu
authors.forename: Jin
authors.initials: J
authors.lastname: Xu
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhang
mesh_terms: D039301:Computers, Molecular; D004247:DNA / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D005456:Fluorescent Dyes / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D008128:Logic; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D036103:Nanotechnology / Q000379:methods
chemical_list: D005456:Fluorescent Dyes; D006046:Gold; D004247:DNA