title: Role of high endothelial venule-expressed heparan sulfate in chemokine presentation and lymphocyte homing.
abstract: Lymphocyte homing to peripheral lymph nodes (PLNs) is mediated by multistep interactions between lymphocytes and high endothelial venules (HEVs). Heparan sulfate (HS) has been implicated in the presentation of chemokines on the surface of HEVs during this process. However, it remains unclear whether this cell surface presentation is a prerequisite for lymphocyte homing. In this study, we generated conditional knockout (cKO) mice lacking Ext1, which encodes a glycosyltransferase essential for HS synthesis, by crossing Ext1(flox/flox) mice with GlcNAc6ST-2-Cre transgenic mice expressing Cre recombinase in HEVs. Immunohistochemical studies indicated that HS expression was specifically eliminated in PLN HEVs but retained in other blood vessels in the cKO mice. The accumulation of a major secondary lymphoid tissue chemokine, CCL21, on HEVs was also abrogated without affecting CCL21 mRNA levels, indicating that HS presents CCL21 on HEVs in vivo. Notably, a short-term lymphocyte homing assay indicated that lymphocyte homing to PLNs was diminished in the cKO mice by 30-40%. Consistent with this result, contact hypersensitivity responses were also diminished in the cKO mice. The residual lymphocyte homing to PLNs in the cKO mice was dependent on pertussis toxin-sensitive Gi protein signaling, in which lysophosphatidic acid-mediated signaling was partly involved. These results suggest that chemokine presentation by HS on the surface of HEVs facilitates but is not absolutely required for lymphocyte homing.
authors.affiliation: Laboratory of Microbiology and Immunology, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka 422-8526, Japan.
authors.forename: Koichiro
authors.initials: K
authors.lastname: Tsuboi
authors.forename: Jotaro
authors.initials: J
authors.lastname: Hirakawa
authors.forename: Emiko
authors.initials: E
authors.lastname: Seki
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Imai
authors.forename: Yu
authors.initials: Y
authors.lastname: Yamaguchi
authors.forename: Minoru
authors.initials: M
authors.lastname: Fukuda
authors.forename: Hiroto
authors.initials: H
authors.lastname: Kawashima
mesh_terms: D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D002465:Cell Movement / Q000276:immunology; D054421:Chemokine CCL21 / Q000378:metabolism; D018925:Chemokines / Q000378:metabolism*; D042783:Endothelial Cells / Q000378:metabolism / Q000502:physiology*; D005260:Female; D006497:Heparan Sulfate / Q000096:biosynthesis / Q000502:physiology*; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology*; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D017351:N-Acetylglucosaminyltransferases / Q000378:metabolism; D016201:Receptors, Lymphocyte Homing / Q000378:metabolism*; D015238:Sulfotransferases / Q000378:metabolism; D014699:Venules / Q000378:metabolism / Q000502:physiology*; D000097811:Carbohydrate Sulfotransferases; D000099065:Exostosin 2; D000099058:Exostosin 1
chemical_list: D054421:Chemokine CCL21; D018925:Chemokines; D006497:Heparan Sulfate; D017351:N-Acetylglucosaminyltransferases; D016201:Receptors, Lymphocyte Homing; D015238:Sulfotransferases; D000097811:Carbohydrate Sulfotransferases; D000099065:Exostosin 2; D000099058:Exostosin 1