title: Rotavirus G2P[4] and G2P[4]+[6] infections during norovirus gastroenteritis outbreak: summer season 2010, Brazil.
abstract: BACKGROUND
  This study aimed to monitor the seasonality of rotavirus infection, and gain insight into the variability of Brazilian strains.
  
  
  METHODS
  A total of 28 stool samples were analyzed from 698 revised cases of gastroenteritis during a norovirus outbreak in the summer of 2010 in Guarujá, Brazil. Diagnosis was performed using enzyme-linked immunosorbent assay (ELISA), reverse transcription polymerase chain reaction (RT-PCR), and sequencing.
  
  
  RESULTS
  Rotavirus infection was detected in 17.9% (5/28) of samples; 4 samples were G2P[4] genotype, and one G2P[4]+P[6] genotype. G2 and P[4] sequences showed a genetic relationship to strains from India and Russia, respectively.
  
  
  CONCLUSIONS
  The seasonal pattern of rotavirus may be a consequence of human activity apart from climate factors.
authors.affiliation: Núcleo de Doenças Entéricas, Centro de Virologia, Instituto Adolfo Lutz, São Paulo, SP, Brasil. driluchs@gmail.com
authors.forename: Adriana
authors.initials: A
authors.lastname: Luchs
authors.forename: Simone Guadagnucci
authors.initials: SG
authors.lastname: Morillo
authors.forename: Cibele Daniel
authors.initials: CD
authors.lastname: Ribeiro
authors.forename: Audrey
authors.initials: A
authors.lastname: Cilli
authors.forename: Samira Julien
authors.initials: SJ
authors.lastname: Calux
authors.forename: Rita de Cássia Compagnoli
authors.initials: Rde C
authors.lastname: Carmona
authors.forename: Maria do Carmo Sampaio Tavares
authors.initials: Mdo C
authors.lastname: Timenetsky
mesh_terms: D000293:Adolescent; D001938:Brazil / Q000453:epidemiology; D017250:Caliciviridae Infections / Q000453:epidemiology*; D004196:Disease Outbreaks; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000821:virology; D005260:Female; D005759:Gastroenteritis / Q000453:epidemiology* / Q000821:virology; D005838:Genotype; D006801:Humans; D008297:Male; D029322:Norovirus / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000235:genetics / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000175:diagnosis / Q000453:epidemiology*; D012621:Seasons