title: Transcriptome sequencing and de novo analysis of a cytoplasmic male sterile line and its near-isogenic restorer line in chili pepper (Capsicum annuum L.).
abstract: BACKGROUND
  The use of cytoplasmic male sterility (CMS) in F1 hybrid seed production of chili pepper is increasingly popular. However, the molecular mechanisms of cytoplasmic male sterility and fertility restoration remain poorly understood due to limited transcriptomic and genomic data. Therefore, we analyzed the difference between a CMS line 121A and its near-isogenic restorer line 121C in transcriptome level using next generation sequencing technology (NGS), aiming to find out critical genes and pathways associated with the male sterility.
  
  
  RESULTS
  We generated approximately 53 million sequencing reads and assembled de novo, yielding 85,144 high quality unigenes with an average length of 643 bp. Among these unigenes, 27,191 were identified as putative homologs of annotated sequences in the public protein databases, 4,326 and 7,061 unigenes were found to be highly abundant in lines 121A and 121C, respectively. Many of the differentially expressed unigenes represent a set of potential candidate genes associated with the formation or abortion of pollen.
  
  
  CONCLUSIONS
  Our study profiled anther transcriptomes of a chili pepper CMS line and its restorer line. The results shed the lights on the occurrence and recovery of the disturbances in nuclear-mitochondrial interaction and provide clues for further investigations.
authors.affiliation: China Agricultural University, Beijing, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Liu
authors.forename: Ning
authors.initials: N
authors.lastname: Ma
authors.forename: Ping-Yong
authors.initials: PY
authors.lastname: Wang
authors.forename: Nan
authors.initials: N
authors.lastname: Fu
authors.forename: Huo-Lin
authors.initials: HL
authors.lastname: Shen
mesh_terms: D002212:Capsicum / Q000166:cytology / Q000235:genetics* / Q000502:physiology*; D003593:Cytoplasm / Q000378:metabolism*; D035264:Flowers / Q000502:physiology; D020869:Gene Expression Profiling; D063990:Gene Ontology; D023281:Genomics; D006824:Hybridization, Genetic; D058977:Molecular Sequence Annotation; D051479:Plant Infertility / Q000235:genetics*; D017423:Sequence Analysis, RNA