title: [Establishment of in vitro co-cultue system for tumor vascular endothelial cells].
abstract: OBJECTIVE
  To establish a in vitro culture model of tumor vascular endothelial cells (TECs).
  
  
  METHODS
  We established in vitro culture model of TECs, named A549-HUVECs, through the co-culutre system culturing primary human umbilical vein endothelial cells (HUVECs) and human lung adenocarcinoma epithelial cell line A549 cells in indirect co-cultures, six-well Transwell-clear dishes (0.4 microm porous filter, Costar). We then observed TECs' cell morphology, growth characteristics, proliferation and karyotype, telomerase activity, etc.
  
  
  RESULTS
  In the indirect co-culture system, A549-HUVECs showed migration cell-type appearance, more active cellular metabolism, increasing cell permeability, augmenting cell proliferation, which indicated A549-HUVECs being in a state of the regulation of homeostasis imbalance for cell proliferation and apoptosis. Besides, the analysis of A549-HUVECs genetic characteristics showed that the chromosome karyotype of A549-HUVECs was sub-triploid, and its telomerase activity increased, phenotypic change occurred, which possessed the similar pathological features of precancerous lesion.
  
  
  CONCLUSIONS
  A549-HUVECs co-culture system could be feasible for tumor vascular research.
authors.affiliation: ICU of Sichuan Cancer Hospital, Chengdu 610041, China.
authors.forename: Song
authors.initials: S
authors.lastname: Lu
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Chen
authors.forename: Ming-Jiang
authors.initials: MJ
authors.lastname: Bie
authors.forename: Jia-Yu
authors.initials: JY
authors.lastname: Wu
authors.forename: Zhong-Hua
authors.initials: ZH
authors.lastname: Xiong
mesh_terms: D045744:Cell Line, Tumor; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology*; D006801:Humans; D008175:Lung Neoplasms / Q000473:pathology*