title: Alternative splicing variants of proinsulin mRNA and the effects of excess proinsulin on cardiac morphogenesis.
abstract: Alternative forms of proinsulin mRNA with differential translational capacities and unknown significance are expressed in several developing tissues and in the adult pancreas. In the chick embryo developing heart, we observed low expression of the translationally active transcript of embryonic proinsulin (Pro1B), and predominant expression of the intron 1-unspliced variant, translationally inactive. In the embryonic mouse heart, intron 1-unspliced isoform appeared after E12.5. This tight regulation is required for normal development, since overexpression of Pro1B resulted in abnormal cardiac morphogenesis in 40% of chick embryos, and was accompanied by changes in gene expression of Amhc1and Vmhc1.
authors.affiliation: 3D (Development, Differentiation, Degeneration) Lab, Centro de Investigaciones Biológicas (CSIC), Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Martínez-Campos
authors.forename: Esther
authors.initials: E
authors.lastname: Hernández-SanMiguel
authors.forename: Carmen
authors.initials: C
authors.lastname: López-Sánchez
authors.forename: Flora
authors.initials: F
authors.lastname: De Pablo
authors.forename: Catalina
authors.initials: C
authors.lastname: Hernández-Sánchez
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D030161:Avian Proteins / Q000235:genetics / Q000378:metabolism; D024722:Cardiac Myosins / Q000235:genetics / Q000378:metabolism; D002642:Chick Embryo; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006321:Heart / Q000196:embryology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009024:Morphogenesis; D011384:Proinsulin / Q000235:genetics* / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D011972:Receptor, Insulin / Q000235:genetics / Q000378:metabolism
chemical_list: D030161:Avian Proteins; D020033:Protein Isoforms; D012333:RNA, Messenger; D011384:Proinsulin; D011972:Receptor, Insulin; D024722:Cardiac Myosins