title: Enterobacter siamensis sp. nov., a transglutaminase-producing bacterium isolated from seafood processing wastewater in Thailand.
abstract: A novel strain of Enterobacter, C2361(T), a Gram-negative, non-spore-forming, rod-shaped and facultative anaerobic bacterium with the capability to produce transglutaminase, was isolated from seafood processing wastewater collected from a treatment pond of a seafood factory in Songkhla Province, Thailand. Phylogenetic analyses and phenotypic characteristics, including chemotaxonomic characteristics, showed that the strain was a member of the genus Enterobacter. The 16S rRNA gene sequence similarities between strain C2361(T) and Enterobacter cloacae subsp. cloacae ATCC 13047(T) and Enterobacter cloacae subsp. dissolvens LMG 2683(T) were 97.5 and 97.5%, respectively. Strain C2361(T) showed a low DNA-DNA relatedness with the above-mentioned species. The major fatty acids were C16:0, C17:0cyclo and C14:0. The DNA G+C content was 53.0 mol%. On the basis of the polyphasic evidence gathered in this study, it should be classified as a novel species of the genus Enterobacter for which the name Enterobacter siamensis sp. nov. is proposed. The type strain is C2361(T) (= KCTC 23282(T) = NBRC 107138(T)).
authors.affiliation: Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
authors.forename: Suwannee
authors.initials: S
authors.lastname: Khunthongpan
authors.forename: Chaiwut
authors.initials: C
authors.lastname: Bourneow
authors.forename: Aran
authors.initials: A
authors.lastname: H-Kittikun
authors.forename: Somboon
authors.initials: S
authors.lastname: Tanasupawat
authors.forename: Soottawat
authors.initials: S
authors.lastname: Benjakul
authors.forename: Punnanee
authors.initials: P
authors.lastname: Sumpavapol
mesh_terms: D000332:Aerobiosis; D000693:Anaerobiosis; D015373:Bacterial Typing Techniques; D001482:Base Composition; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004754:Enterobacter / Q000145:classification* / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D005227:Fatty Acids / Q000032:analysis; D007220:Industrial Waste; D008855:Microscopy, Electron, Scanning; D008858:Microscopy, Phase-Contrast; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017747:Seafood; D017422:Sequence Analysis, DNA; D013785:Thailand; D011503:Transglutaminases / Q000378:metabolism*; D062065:Wastewater / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D007220:Industrial Waste; D012336:RNA, Ribosomal, 16S; D011503:Transglutaminases; D062065:Wastewater