title: Naturally occurring ERAP1 haplotypes encode functionally distinct alleles with fine substrate specificity.
abstract: Endoplasmic reticulum aminopeptidase 1 (ERAP1) trims peptides for MHC class I presentation, influencing the degree and specificity of CD8(+) T cell responses. Single-nucleotide polymorphisms within the exons encoding ERAP1 are associated with autoimmune diseases and cervical carcinoma, but it is not known whether they act independently or as disease-associated haplotypes. We sequenced ERAP1 from 20 individuals and show that single-nucleotide polymorphisms occur as distinct haplotypes in the human population and that these haplotypes encode functionally distinct ERAP1 alleles. Using a wide range of substrates, we are able to demonstrate that for any given substrate distinct ERAP1 alleles can be "normal," "hypofunctional," or "hyperfunctional" and that each allele has a trend bias toward one of these three activities. Thus, the repertoire of peptides presented at the cell surface for recognition by CTL is likely to depend on the precise combination of both MHC class I and ERAP1 alleles expressed within an individual, and has important implications for predisposition to disease.
authors.affiliation: Cancer Sciences Unit, Faculty of Medicine, University of Southampton, Southampton, United Kingdom.
authors.forename: Emma
authors.initials: E
authors.lastname: Reeves
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Edwards
authors.forename: Tim
authors.initials: T
authors.lastname: Elliott
authors.forename: Edward
authors.initials: E
authors.lastname: James
mesh_terms: D000483:Alleles; D000626:Aminopeptidases / Q000235:genetics*; D001327:Autoimmune Diseases / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D002460:Cell Line; D005260:Female; D006239:Haplotypes / Q000235:genetics* / Q000276:immunology; D006801:Humans; D015778:Minor Histocompatibility Antigens; D010455:Peptides / Q000235:genetics / Q000378:metabolism; D020641:Polymorphism, Single Nucleotide / Q000235:genetics / Q000276:immunology; D013379:Substrate Specificity / Q000235:genetics / Q000276:immunology; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000276:immunology / Q000378:metabolism
chemical_list: D015778:Minor Histocompatibility Antigens; D010455:Peptides; D000626:Aminopeptidases; C464841:ERAP1 protein, human