title: Proteomic analysis revealed the altered tear protein profile in a rabbit model of Sjögren's syndrome-associated dry eye.
abstract: Sjögren's syndrome (SS) is an autoimmune disease that results in pathological dryness of mouth and eye. The diagnosis of SS depends on both clinical evaluation and specific antibodies. The goal of this study was to use quantitative proteomics to investigate changes in tear proteins in a rabbit model of SS-associated dry eye, induced autoimmune dacryoadenitis (IAD). Proteomic analysis was performed by iTRAQ and nano LC-MS/MS on tears collected from the ocular surface, and specific proteins were verified by high resolution MRM. It was found that in the tears of IAD rabbits at 2 and 4 weeks after induction, S100 A6, S100 A9, and serum albumin were upregulated, whereas serotransferrin (TF), prolactin-inducible protein (PIP), polymeric immunoglobulin receptor (pIgR), and Ig gamma chain C region were downregulated. High resolution MRM with mTRAQ labeling verified the changes in S100 A6, TF, PIP, and pIgR. Our results indicated significant changes of tear proteins in IAD rabbits, suggesting these proteins could potentially be used as biomarkers for the diagnosis and prognosis of dry eye. Several of these proteins were also found in the tears of non-SS dry eye patients indicating a common basis of ocular surface pathology, however, pIgR appears to be unique to SS.
authors.affiliation: Singapore Eye Research Institute, Singapore. zhou.lei@seri.com.sg
authors.forename: Lei
authors.initials: L
authors.lastname: Zhou
authors.forename: Ruihua
authors.initials: R
authors.lastname: Wei
authors.forename: Ping
authors.initials: P
authors.lastname: Zhao
authors.forename: Siew Kwan
authors.initials: SK
authors.lastname: Koh
authors.forename: Roger W
authors.initials: RW
authors.lastname: Beuerman
authors.forename: Chuanqing
authors.initials: C
authors.lastname: Ding
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002853:Chromatography, Liquid; D003607:Dacryocystitis / Q000378:metabolism; D004195:Disease Models, Animal; D015352:Dry Eye Syndromes / Q000378:metabolism*; D005136:Eye Proteins / Q000032:analysis* / Q000737:chemistry / Q000145:classification; D005260:Female; D007765:Lacrimal Apparatus / Q000473:pathology; D008969:Molecular Sequence Data; D020543:Proteome / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics; D011817:Rabbits; D015203:Reproducibility of Results; D012859:Sjogren's Syndrome / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D013666:Tears / Q000737:chemistry*
chemical_list: D005136:Eye Proteins; D020543:Proteome; C042434:tear proteins
keywords: Animal proteomics; Dry eye; High resolution MRM; Sjögren's Syndrome; Tear proteomics; mTRAQ