title: Carboligation reactions mediated by benzoylformate decarboxylase immobilized on a magnetic solid support.
abstract: In this study, magnetic nanoparticles (Fe3 O4 , magnetite) with immobilized metal affinity ligands (MSS) were prepared and characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), fourier transform infrared spectroscopy (FTIR), and vibrating-sample magnetometer (VSM) methods for purification and immobilization of the histidine-tagged recombinant benzoylformate decarboxylase (BFD). The MSS support was shown to be eligible for selective binding of HIS-tagged BFD by SDS-page analysis. Loading capacity of the MSS support was determined as 43.6 ± 1.1 mg/g. The regeneration ability for protein binding was also studied. An immobilized BFD was tested to catalyze benzoin condensation and representative cross acyloin reaction. Conversion and enantiomeric excess values were comparable with that of free enzyme catalyzed reactions.
authors.affiliation: Department of Chemistry, Dicle University, Diyarbakir, Turkey. bilsentural@gmail.com
authors.forename: Bilsen
authors.initials: B
authors.lastname: Tural
authors.forename: Servet
authors.initials: S
authors.lastname: Tural
authors.forename: Ayhan S
authors.initials: AS
authors.lastname: Demir
mesh_terms: D001573:Benzoin / Q000737:chemistry; D002262:Carboxy-Lyases / Q000737:chemistry* / Q000378:metabolism*; D003300:Copper / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D004800:Enzymes, Immobilized; D058185:Magnetite Nanoparticles; D012822:Silicon Dioxide; D003300:Copper; D002262:Carboxy-Lyases; C019778:benzoylformate decarboxylase; D001573:Benzoin
keywords: benzoylformate decarboxylase (BFD); carboligation reactions; his-tagged protein purification; metal-affinity magnetic nanoparticles