title: Halobellus inordinatus sp. nov., from a marine solar saltern and an inland salt lake of China.
abstract: Two halophilic archaeal strains, YC20(T) and XD15, were isolated from a marine solar saltern and an inland salt lake in China. Both had pleomorphic cells that lysed in distilled water, stained Gram-negative and formed red-pigmented colonies. They were neutrophilic, requiring at least 100 g NaCl l(-1) and 0.5-95 g MgCl2 l(-1) for growth at the optimum growth temperature of 37 °C. The major polar lipids of the two strains were phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), phosphatidylglycerol sulfate (PGS) and two major glycolipids chromatographically identical to sulfated mannosyl glucosyl diether (S-DGD-1) and mannosyl glucosyl diether (DGD-1), respectively. Trace amounts of two unidentified glycolipids were also detected. The 16S rRNA gene sequences of the two strains were 99.5 % identical and showed 94.0-95.9 % similarity to the most closely related members of the genus Halobellus of the family Halobacteriaceae. The rpoB' gene sequence similarity between strains YC20(T) and XD15 was 98.2 % and these sequences showed 89.6-92.8 % similarity to those of the most closely related members of the genus Halobellus. The DNA G+C contents of strains YC20(T) and XD15 were 65.8 mol% and 65.4 mol%, respectively. The DNA-DNA hybridization value between strain YC20(T) and strain XD15 was 92 %, and the two strains showed low DNA-DNA relatedness to members of the genus Halobellus. The phenotypic, chemotaxonomic and phylogenetic properties suggest that strains YC20(T) and XD15 represent a novel species of the genus Halobellus, for which the name Halobellus inordinatus sp. nov. is proposed. The type strain is YC20(T) ( = CGMCC 1.12120(T) = JCM 18361(T)) and the other strain is XD15 ( = CGMCC 1.12236 = JCM 18648).
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Xing-Xing
authors.initials: XX
authors.lastname: Qiu
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Yun-Zhuang
authors.initials: YZ
authors.lastname: Mou
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Mei-Lin
authors.initials: ML
authors.lastname: Zhao
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Wen-Jiao
authors.initials: WJ
authors.lastname: Zhang
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Han
authors.affiliation: Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production and Construction Corps, Tarim University, Alar 843300, PR China.
authors.forename: Min
authors.initials: M
authors.lastname: Ren
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Heng-Lin
authors.initials: HL
authors.lastname: Cui
mesh_terms: D001482:Base Composition; D002681:China; D019641:DNA, Archaeal / Q000235:genetics; D019766:Genes, Archaeal; D006017:Glycolipids / Q000737:chemistry; D006216:Halobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D060106:Lakes / Q000382:microbiology*; D015636:Magnesium Chloride; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012492:Salts; D012965:Sodium Chloride; D013696:Temperature; D014871:Water Microbiology
chemical_list: D019641:DNA, Archaeal; D006017:Glycolipids; D012336:RNA, Ribosomal, 16S; D012492:Salts; C017082:brine; D015636:Magnesium Chloride; D012965:Sodium Chloride