title: Orally active hypoglycemic protein from Costus igneus N. E. Br.: an in vitro and in vivo study.
abstract: Plants have been used for the treatment of diabetes since time immemorial. In the present study, insulin-like protein (ILP) is purified from Costus igneus belonging to family Costaceae from Western ghats of India. The ILP showed cross reactivity with murine anti-insulin antibodies hence was purified by affinity chromatography using anti-insulin antibodies. The characterization of ILP showed that it is structurally different from insulin but functionally similar. The ILP showed a hypoglycemic activity in an in vitro assay with insulin responsive cell line RIN 5f. Interestingly ILP showed significant decrease in blood glucose level when administered orally in oral glucose tolerance test. This was compared to insulin a positive control given intraperitoneally in streptozotocine induced diabetic mice. There was no toxic effect seen on animals after administrating the ILP. Therefore we conclude that the ILP purified in the present study from C. igneus is a novel protein having hypoglycemic activity.
authors.affiliation: Biometry and Nutrition Group, Agharkar Research Institute, Pune 411 004, India. bimbajoshi@aripune.org
authors.forename: Bimba N
authors.initials: BN
authors.lastname: Joshi
authors.forename: Hitendra
authors.initials: H
authors.lastname: Munot
authors.forename: Manasi
authors.initials: M
authors.lastname: Hardikar
authors.forename: Anjali A
authors.initials: AA
authors.lastname: Kulkarni
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D015153:Blotting, Western; D045744:Cell Line, Tumor; D029770:Costus / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000517:prevention & control; D005947:Glucose / Q000378:metabolism / Q000493:pharmacokinetics; D005951:Glucose Tolerance Test; D007004:Hypoglycemic Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D007274:Injections, Intraperitoneal; D007328:Insulin / Q000008:administration & dosage / Q000494:pharmacology; D007515:Islets of Langerhans / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D051379:Mice; D036361:Peptide Hormones / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D008517:Phytotherapy / Q000379:methods; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000302:isolation & purification / Q000494:pharmacology; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D001786:Blood Glucose; D007004:Hypoglycemic Agents; D007328:Insulin; D036361:Peptide Hormones; D010936:Plant Extracts; D010937:Plant Growth Regulators; D010940:Plant Proteins; D005947:Glucose