title: Expression of tissue IGF 1, TGFbeta and EGFR in the sequential steps of intestinal adaptation in a rat model of short bowel syndrome.
abstract: BACKGROUND
  The long-term morphological changes and the expression of tissue growth factors IGF 1, TGFbeta and EGFR in the gut mucosa, during the process of intestinal adaptation were examined.
  
  
  METHODS
  Four groups of rats were used: a. Sham rats (n = 10) underwent bowel transection and reanastomosis, b. SBS rats underwent an 80% small bowel resection: group A rats (n = 10) were sacrificed 15 days after surgery, group B (n = 10), 30 days after surgery, and group C (n = 10), 60 days after surgery. Morphological small bowel parameters (villus height, lumen diameter and others) of adaptation were examined sequentially. Tissue samples were studied immunohistochemically for the detection of IGF 1, TGFbeta, and EGFR.
  
  
  RESULTS
  There was a significant increase in all morphological parameters at day 15, in the intestinal samples; a further increase followed at day 30 and day 60 (p < 0.0001). Accordingly, an increase in the expression of IGF 1, TGFbeta and EGFR was noted at day 15 (p < 0.05), and at day 30 (NS).
  
  
  CONCLUSIONS
  Intestinal adaptation is an ongoing process lasting more than 2 months after massive small bowel resection. Peptide growth factors are expressed in the intestine continuously during this period, but the first two weeks are the most critical for the mucosal growth.
authors.affiliation: 3rd Department of Surgery, University of Athens School of Medicine, Attikon University Hospital, Athens, Greece. misiakos@med.uoa.gr
authors.forename: E P
authors.initials: EP
authors.lastname: Misiakos
authors.forename: G
authors.initials: G
authors.lastname: Agrogiannis
authors.forename: P
authors.initials: P
authors.lastname: Patapis
authors.forename: I
authors.initials: I
authors.lastname: Dontas
authors.forename: K
authors.initials: K
authors.lastname: Petropoulos
authors.forename: N
authors.initials: N
authors.lastname: Machairas
authors.forename: E
authors.initials: E
authors.lastname: Giamarellos-Bourboulis
authors.forename: T
authors.initials: T
authors.lastname: Liakakos
authors.forename: A
authors.initials: A
authors.lastname: Machairas
mesh_terms: D000222:Adaptation, Physiological / Q000502:physiology*; D000818:Animals; D004195:Disease Models, Animal; D066246:ErbB Receptors / Q000378:metabolism*; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D007421:Intestine, Small / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012778:Short Bowel Syndrome / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors; D016212:Transforming Growth Factor beta / Q000378:metabolism*
chemical_list: D016212:Transforming Growth Factor beta; D007334:Insulin-Like Growth Factor I; D066246:ErbB Receptors