title: Characterization of a novel cell line from the caudal fin of koi carp Cyprinus carpio.
abstract: A continuous cell line (KF-101) derived from the caudal fin of the koi carp Cyprinus carpio was established and characterized. The KF-101 cell line multiplied abundantly in Leibovitz's L-15 medium containing 10% foetal bovine serum at 25° C, and was subcultured for >90 passages over a period of 3 years. Immunocytochemistry revealed that the KF-101 cells contain keratin, junction proteins connexin-43 and occludin, and ectodermal stem-cell marker Pax-6, but not vimentin. Furthermore, the KF-101 cells reacted with anti-human DARPP-32 and anti-human GATA-4 antibodies, and the labelling was regulated according to the cell cycle. The labels of the DARPP-32 and GATA-4 antibodies in the KF-101 cells were the suggested phosphatase-1 inhibitor-1 and GATA-3, respectively. In addition, the KF-101 cells were susceptible to koi herpesvirus but were resistant to eel herpesvirus, iridovirus, grouper nodavirus and chum salmon (Oncorhynchus keta) virus. The results indicate that the KF-101 cells are suitable materials for investigating biological and virological development.
authors.affiliation: Institute of Fisheries Science, National Taiwan University, Taipei, Taiwan, China.
authors.forename: S-L
authors.initials: SL
authors.lastname: Lin
authors.forename: Y-H
authors.initials: YH
authors.lastname: Cheng
authors.forename: C-M
authors.initials: CM
authors.lastname: Wen
authors.forename: S-N
authors.initials: SN
authors.lastname: Chen
mesh_terms: D058500:Animal Fins / Q000166:cytology* / Q000821:virology; D000818:Animals; D002347:Carps / Q000821:virology*; D018929:Cell Culture Techniques; D002460:Cell Line; D049109:Cell Proliferation; D006564:Herpesviridae / Q000502:physiology; D007497:Iridoviridae / Q000502:physiology; D012087:Reoviridae / Q000502:physiology