title: [Construction and expression of recombinant lentivirus encoding miR-203 and its inhibitory effect on proliferation and migration of human umbilical vein endothelial cells].
abstract: OBJECTIVE
  To construct a recombinant lentivirus harboring human-derived microRNA-203 (miR-203), and investigate its biological effect on proliferation and migration of human umbilical vein endothelial cells (HUVEC-12).
  
  
  METHODS
  The precursor miR-203 DNA fragment was obtained by PCR and inserted into pLenti-6.3 lentivirus vector, and then was stably transfected into HUVEC-12 cells (HUVEC-Lv-miR-203). Overexpression of exogenous pre-miR-203 was determined using real-time quantitative PCR (qRT-PCR). Subsequently, to investigate its effects on proliferation and migration of HUVEC-12 cells, MTT assay and wound healing assay were respectively performed.
  
  
  RESULTS
  We successfully constructed a lentivirus-based delivery system to stably express exogenous pre-miR-203 in HUVEC-12 cells. MTT assay and wound healing assay showed that miR-203 could significantly suppress cell proliferation and migration of HUVEC-12.
  
  
  CONCLUSIONS
  MiR-203 has the inhibitory effect on the proliferation and migration of HUVEC-12 cells.
authors.affiliation: State Key Laboratory of Cancer Biology, Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Huayu
authors.initials: H
authors.lastname: Zhu
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhang
authors.forename: Xiao
authors.initials: X
authors.lastname: Zhang
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.forename: Angang
authors.initials: A
authors.lastname: Yang
mesh_terms: D002460:Cell Line; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism*; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D014162:Transfection
chemical_list: C546703:MIRN203 microRNA, human; D035683:MicroRNAs