title: Critical role of transient activity of MT1-MMP for ECM degradation in invadopodia.
abstract: Focal degradation of extracellular matrix (ECM) is the first step in the invasion of cancer cells. MT1-MMP is a potent membrane proteinase employed by aggressive cancer cells. In our previous study, we reported that MT1-MMP was preferentially located at membrane protrusions called invadopodia, where MT1-MMP underwent quick turnover. Our computer simulation and experiments showed that this quick turnover was essential for the degradation of ECM at invadopodia (Hoshino, D., et al., (2012) PLoS Comp. Biol., 8: e1002479). Here we report on characterization and analysis of the ECM-degrading activity of MT1-MMP, aiming at elucidating a possible reason for its repetitive insertion in the ECM degradation. First, in our computational model, we found a very narrow transient peak in the activity of MT1-MMP followed by steady state activity. This transient activity was due to the inhibition by TIMP-2, and the steady state activity of MT1-MMP decreased dramatically at higher TIMP-2 concentrations. Second, we evaluated the role of the narrow transient activity in the ECM degradation. When the transient activity was forcibly suppressed in computer simulations, the ECM degradation was heavily suppressed, indicating the essential role of this transient peak in the ECM degradation. Third, we compared continuous and pulsatile turnover of MT1-MMP in the ECM degradation at invadopodia. The pulsatile insertion showed basically consistent results with the continuous insertion in the ECM degradation, and the ECM degrading efficacy depended heavily on the transient activity of MT1-MMP in both models. Unexpectedly, however, low-frequency/high-concentration insertion of MT1-MMP was more effective in ECM degradation than high-frequency/low-concentration pulsatile insertion even if the time-averaged amount of inserted MT1-MMP was the same. The present analysis and characterization of ECM degradation by MT1-MMP together with our previous report indicate a dynamic nature of MT1-MMP at invadopodia and the importance of its transient peak in the degradation of the ECM.
authors.affiliation: Division of Mathematical Oncology, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo, Japan.
authors.forename: Ayako
authors.initials: A
authors.lastname: Watanabe
authors.forename: Daisuke
authors.initials: D
authors.lastname: Hoshino
authors.forename: Daisuke
authors.initials: D
authors.lastname: Hosino
authors.forename: Naohiko
authors.initials: N
authors.lastname: Koshikawa
authors.forename: Motoharu
authors.initials: M
authors.lastname: Seiki
authors.forename: Takashi
authors.initials: T
authors.lastname: Suzuki
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Ichikawa
mesh_terms: D045744:Cell Line, Tumor; D019295:Computational Biology; D003198:Computer Simulation; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D053511:Matrix Metalloproteinase 14 / Q000737:chemistry / Q000378:metabolism*; D008954:Models, Biological; D009385:Neoplastic Processes
chemical_list: D053511:Matrix Metalloproteinase 14