title: [Role of BPIFB1 in regulating inflammatory response of RAW264.7 cells infected by P.aeruginosa].
abstract: OBJECTIVE
  To demonstrate the mechanisms and regulation pathways of BPIFB1 against P.aeruginosa infection.
  
  
  METHODS
  After treated with 10 ng/mL LPS and 1.28 mg/mL BPIFB1, RAW 246.7 cells were detected for the expression levels of CD14 receptor, TLR4 and MyD88 by ELISA and Western blotting. In addition, using RNAi and specific protease inhibitor blockade respectively, we observed the changes in the expressions of MyD88, TRAF6, NF-κB and different signaling pathway molecules in RAW 246.7 cells after BPIFB1 and LPS treatment.
  
  
  RESULTS
  The expression levels of CD14, TLR4 and MyD88 significantly decreased after the RAW264.7 cells were treated with BPIFB1 and LPS. When the expressions of MyD88, TRAF6 and NF-κB were blocked by specific siRNA respectively, there was a significant reduction of TNF-α over-expression induced by BPIFB1 and LPS. And when the cells were treated with BPIFB1, LPS and related protease inhibitors, the phosphorylation levels of ERK1/2, p38 and Akt1 were evidently inhibited by kinase inhibitors.
  
  
  CONCLUSIONS
  The combination of BPIFB1 and LPS can reduce the degree of cell inflammatory responses induced by P.aeruginosa by inhibiting the expressions of relative intracellular cytokines.
authors.affiliation: Department of Pathogen Biology, Shenyang Medical College, Shenyang 110034, China. zhouwq@hotmail.com
authors.forename: Weiqiang
authors.initials: W
authors.lastname: Zhou
authors.forename: Xiuyan
authors.initials: X
authors.lastname: Feng
authors.forename: Chunling
authors.initials: C
authors.lastname: Xiao
authors.forename: Shuyin
authors.initials: S
authors.lastname: Li
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Wang
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D007249:Inflammation / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology / Q000378:metabolism; D008264:Macrophages / Q000276:immunology / Q000378:metabolism* / Q000382:microbiology*; D051379:Mice; D053594:Myeloid Differentiation Factor 88 / Q000378:metabolism; D010766:Phosphorylation; D011550:Pseudomonas aeruginosa / Q000502:physiology*; D034622:RNA Interference; D015398:Signal Transduction; D051197:Toll-Like Receptor 4 / Q000378:metabolism
chemical_list: C579352:BPIFB1 protein, mouse; D002352:Carrier Proteins; D018950:Lipopolysaccharide Receptors; D008070:Lipopolysaccharides; D053594:Myeloid Differentiation Factor 88; D051197:Toll-Like Receptor 4