title: Hydrogen peroxide is involved in cGMP modulating the lateral root development of Arabidopsis thaliana.
abstract: 3',5'-cyclic guanosine monophosphate (cGMP) and hydrogen peroxide (H₂O₂) function as the important signaling molecule which promote the lateral root development of Arabidopsis thaliana. In this study, interestingly, application of 8-Br-cGMP (the membrane permeable cGMP analog) promoted the endogenous H₂O₂ production. In addition, the decrease of endogenous H₂O₂ also inhibited the effect of cGMP on the lateral root development. Thus, H₂O₂ maybe act as a downstream signaling of cGMP molecule which is involved in the lateral root development of Arabidopsis. We further found that H₂O₂ affected cGMP modulating polar auxin transport. When the endogenous H₂O₂ level was inhibited, the effect of cGMP on the acropetal auxin transport and the basipetal auxin transport was removed. Moreover, pin2 was insensitive for cGMP and H₂O₂ suggesting that PIN2 protein plays an important role in cGMP and H₂O₂ modulating the lateral root development of Arabidopsis.
authors.affiliation: College of Life Sciences; Northwest A&F University; Yangling, Shaanxi P.R. China.
authors.forename: Jisjeng
authors.initials: J
authors.lastname: Li
authors.forename: Honglei
authors.initials: H
authors.lastname: Jia
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D001692:Biological Transport / Q000187:drug effects; D006152:Cyclic GMP / Q000031:analogs & derivatives* / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D018517:Plant Roots / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013873:Thionucleotides / Q000494:pharmacology*
chemical_list: D013873:Thionucleotides; C083763:8-bromoguanosino-3',5'-cyclic monophosphorothioate; D006861:Hydrogen Peroxide; D006152:Cyclic GMP