title: Integrated analysis of genomics and proteomics reveals that CKIP-1 is a novel macrophage migration regulator.
abstract: Casein kinase-2 interacting protein-1 (CKIP-1) has been identified to play an important role in cell morphology, differentiation and apoptosis. However, the role of CKIP-1 in other cellular processes is still unknown. Here we investigated transcriptome profiles of WT and CKIP-1-deficient mouse embryonic fibroblasts (MEFs), and found that innate immunity and cell migration related pathways were significantly correlated with CKIP-1 expression. As macrophage is a key cell type in innate immunity, we then used murine macrophage RAW264.7 cells to discover CKIP-1 interacting proteins by immunoprecipitation/mass spectrometry (IP/MS). Analysis of these proteins revealed migration related pathways were enriched. Further experiments indicated that knockdown of CKIP-1 in RAW264.7 cells resulted in impaired cell migration. Our study suggests that CKIP-1 is a novel regulator of macrophage migration.
authors.affiliation: Department of Biomedical Engineering, Chinese PLA 307 Hospital, Beijing 100071, China.
authors.forename: Luo
authors.initials: L
authors.lastname: Zhang
authors.forename: Xia
authors.initials: X
authors.lastname: Xia
authors.forename: Minghua
authors.initials: M
authors.lastname: Zhang
authors.forename: Yiwu
authors.initials: Y
authors.lastname: Wang
authors.forename: Guichun
authors.initials: G
authors.lastname: Xing
authors.forename: Xiushan
authors.initials: X
authors.lastname: Yin
authors.forename: Lei
authors.initials: L
authors.lastname: Song
authors.forename: Fuchu
authors.initials: F
authors.lastname: He
authors.forename: Lingqiang
authors.initials: L
authors.lastname: Zhang
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D054442:Cell Migration Assays, Macrophage; D002465:Cell Movement; D002630:Chemotactic Factors / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism; D055786:Gene Knockout Techniques; D023281:Genomics; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D009240:N-Formylmethionine Leucyl-Phenylalanine / Q000494:pharmacology; D025941:Protein Interaction Mapping; D040901:Proteomics; D015398:Signal Transduction; D059467:Transcriptome; D047908:Intracellular Signaling Peptides and Proteins
chemical_list: D002352:Carrier Proteins; D002630:Chemotactic Factors; D009240:N-Formylmethionine Leucyl-Phenylalanine; C000620556:PLEKHO1 protein, mouse; D047908:Intracellular Signaling Peptides and Proteins
keywords: CKIP-1; IP/MS; Macrophage migration; Microarray