title: Characterization of peripheral blood stem cell grafts mobilized by granulocyte colony-stimulating factor and plerixafor compared with granulocyte colony-stimulating factor alone.
abstract: OBJECTIVE
  This study aimed to characterize the immune effectors contained in apheresis samples obtained from patients with grafts mobilized with plerixafor and granulocyte colony-stimulating factor (G-CSF) (P+G) compared with grafts mobilized with G-CSF alone (G).
  
  
  METHODS
  Aliquots of apheresis samples were obtained from 36 patients with malignant diseases after mobilization with G (n = 18) or P+G (n = 18). The phenotype and cytokine secretion profile of T cell and dendritic cell subsets were characterized by multicolor cytometry including intracellular cytokine staining.
  
  
  RESULTS
  In grafts collected after mobilization with P+G, there was a significantly higher percentage of CD3(+) T cells compared with samples collected after mobilization with G alone. On a functional level, a significant increase of interferon-γ and tumor necrosis factor-α secreting CD8(+) T cells was observed in the P+G group compared with the G group. CD4(+)Foxp3(+) regulatory T cells were similar in both groups but exhibited a lower expression of inducible costimulatory molecule and a significantly higher expression of CD127 in the P+G group. Myeloid dendritic cells (MDCs) and BDCA3(+) dendritic cells were similar in both groups. In contrast, plasmacytoid dendritic cells (PDCs) (CD123(+)BDCA2(+)HLA-DR(+)) were significantly increased in the P+G grafts, leading to a higher PDC-to-MDC ratio. PDCs mobilized by P+G displayed different functional markers--a higher percentage of ILT7(+) PDCs and decreased expression of CD86--suggesting a potential regulatory capacity of PDCs mobilized by P+G.
  
  
  CONCLUSIONS
  Grafts mobilized with P+G exhibited major different functional features compared with grafts mobilized with G alone, suggesting that such grafts may have an impact on patient outcome after autologous stem cell transplantation.
authors.affiliation: EFS Bourgogne Franche-Comté, Besançon, France. beatrice.gaugler@efs.sante.fr
authors.forename: Beatrice
authors.initials: B
authors.lastname: Gaugler
authors.forename: Jessy
authors.initials: J
authors.lastname: Arbez
authors.forename: Steven
authors.initials: S
authors.lastname: Legouill
authors.forename: Pierre
authors.initials: P
authors.lastname: Tiberghien
authors.forename: Philippe
authors.initials: P
authors.lastname: Moreau
authors.forename: Sophie
authors.initials: S
authors.lastname: Derenne
authors.forename: Philippe
authors.initials: P
authors.lastname: Saas
authors.forename: Mohamad
authors.initials: M
authors.lastname: Mohty
mesh_terms: D000293:Adolescent; D000328:Adult; D000954:Antigens, Surface / Q000378:metabolism; D001596:Benzylamines; D001781:Blood Component Removal / Q000379:methods; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D000080027:Cyclams; D003713:Dendritic Cells / Q000166:cytology* / Q000378:metabolism; D005260:Female; D051858:Forkhead Transcription Factors / Q000378:metabolism; D016179:Granulocyte Colony-Stimulating Factor / Q000008:administration & dosage / Q000378:metabolism*; D006684:HLA-DR Antigens / Q000276:immunology; D006571:Heterocyclic Compounds / Q000008:administration & dosage / Q000378:metabolism*; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D036102:Peripheral Blood Stem Cell Transplantation; D018180:Thrombomodulin; D019737:Transplants / Q000187:drug effects / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000954:Antigens, Surface; D001596:Benzylamines; D000080027:Cyclams; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D006684:HLA-DR Antigens; D006571:Heterocyclic Compounds; C546931:THBD protein, human; D018180:Thrombomodulin; D014409:Tumor Necrosis Factor-alpha; D016179:Granulocyte Colony-Stimulating Factor; D007371:Interferon-gamma; C088327:plerixafor