title: Dicentrine production in callus and cell suspension cultures of Stephania venosa.
abstract: The highest dicentrine content (19.5 +/- 0.3 mg/g dry weight) from callus culture of Stephania venosa was achieved from stem segments cultured on MS medium supplemented with TDZ 0.5 mg/L and NAA 1.0 mg/L. Cell suspension cultures were established from callus cultured on MS liquid medium with the same plant growth regulators. Dicentrine production from S. venosa cell suspension cultures was obtained in the range of 15-26 mg/g dry weight. Elicitation in cell suspension cultures by chitosan (50 mg/L) and salicylic acid (2 mg/L) for 6 days significantly increased dicentrine content. Our findings indicate that callus and cell suspension cultures of S. venosa can produce high levels of dicentrine as an alternative source of plant materials.
authors.affiliation: Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, Thailand 40002.
authors.forename: Tharita
authors.initials: T
authors.lastname: Kitisripanya
authors.forename: Jukrapun
authors.initials: J
authors.lastname: Komaikul
authors.forename: Nirachara
authors.initials: N
authors.lastname: Tawinkan
authors.forename: Chuennapha
authors.initials: C
authors.lastname: Atsawinkowit
authors.forename: Waraporn
authors.initials: W
authors.lastname: Putalun
mesh_terms: D001060:Aporphines / Q000378:metabolism*; D031607:Stephania / Q000254:growth & development / Q000378:metabolism*; D013535:Suspensions
chemical_list: D001060:Aporphines; D013535:Suspensions; C067342:dicentrine