title: Biological and Molecular Characterization of Cucumber mosaic virus Isolate Causing Shoestring Disease of Tomato in India which has Closer Affinity to European or East Asian Isolates of CMV.
abstract: An isolate of Cucumber mosaic virus (CMV) retrieved from tomato in India was characterized based on its transmission by sap inoculations, Western blot immuno-assay and sequence analysis of RNA3 and 2b genome. The RNA3 genome was of 2,220 nucleotides (nt) which contained two ORFs: movement protein of 852 nt translating 283 amino acids and coat protein of 657 nt translating 218 amino acids. The complete sequence of RNA3 geneome (Acc. EF153734) shared highest 98-99% identities with P1-1, Tfn, and Nt9 strains of CMV infecting tomato reported from abroad. The 333 nucleotides long RNA2b gene (Acc. EF710773) also showed highest 98% identities with P1-1 and 97% with Tfn and NT9 strains of CMV but only 83-84% identities with Indian strains of CMV. Therefore, the isolate under study was identified as a new isolate of CMV of subgroup IB based on highest sequence similarities and closer affinity to European or East Asian isolates of CMV.
authors.affiliation: Plant Molecular Virology, CSIR-National Botanical Research Institute, Lucknow, 226 001 India.
authors.forename: D
authors.initials: D
authors.lastname: Pratap
authors.forename: S
authors.initials: S
authors.lastname: Kumar
authors.forename: S K
authors.initials: SK
authors.lastname: Snehi
authors.forename: S K
authors.initials: SK
authors.lastname: Raj
keywords: Cucumber mosaic virus; Distinct isolate; Molecular characterization; Tomato