title: Single mutations introduced in the essential ribosomal proteins L3 and S10 cause a sporulation defect in Bacillus subtilis.
abstract: We introduced single mutations into the rplC and rpsJ genes, which encode the essential ribosomal proteins L3 (RplC) and S10 (RpsJ), respectively, and are located in the S10 gene cluster of the gram-positive, endospore-forming bacterium Bacillus subtilis, and examined whether these mutations affected their growth rate, sporulation, competence development and 70S ribosome formation. Mutant cells harboring the G52D mutation in the L3 ribosomal protein, which is located at the peptidyl transferase center of 50S, accumulated 30S subunit at 45°C, probably due to a defect in 50S formation, and exhibited a reduction in the sporulation frequency at high temperature. On the other hand, mutant cells harboring the H56R mutation in the S10 protein, which is located near the aminoacyl-tRNA site of 30S, showed severe growth defect and deficiency in spore formation, and also exhibited significant delay in competence development.
authors.affiliation: Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University, Bunkyo-ku, Tokyo 112-8551, Japan.
authors.forename: Genki
authors.initials: G
authors.lastname: Akanuma
authors.forename: Shota
authors.initials: S
authors.lastname: Suzuki
authors.forename: Koichi
authors.initials: K
authors.lastname: Yano
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nanamiya
authors.forename: Yousuke
authors.initials: Y
authors.lastname: Natori
authors.forename: Eri
authors.initials: E
authors.lastname: Namba
authors.forename: Kazuya
authors.initials: K
authors.lastname: Watanabe
authors.forename: Kazumi
authors.initials: K
authors.lastname: Tagami
authors.forename: Takuya
authors.initials: T
authors.lastname: Takeda
authors.forename: Yuka
authors.initials: Y
authors.lastname: Iizuka
authors.forename: Ako
authors.initials: A
authors.lastname: Kobayashi
authors.forename: Morio
authors.initials: M
authors.lastname: Ishizuka
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Yoshikawa
authors.forename: Fujio
authors.initials: F
authors.lastname: Kawamura
mesh_terms: D001412:Bacillus subtilis / Q000235:genetics* / Q000254:growth & development*; D020125:Mutation, Missense; D000090822:Ribosomal Protein L3; D012269:Ribosomal Proteins / Q000235:genetics*; D013171:Spores, Bacterial / Q000235:genetics* / Q000254:growth & development*
chemical_list: D000090822:Ribosomal Protein L3; D012269:Ribosomal Proteins; C029537:ribosomal protein S10