title: Spontaneous mutation 7B-1 in tomato impairs blue light-induced stomatal opening.
abstract: It was reported earlier that 7B-1 mutant in tomato (Solanum lycopersicum L.), an ABA overproducer, is defective in blue light (BL) signaling leading to BL-specific resistance to abiotic and biotic stresses. In this work, we examine responses of stomata to blue, red and white lights, fusicoccin, anion channel blockers (anthracene-9-carboxylic acid; 9-AC and niflumic acid; NIF) and ABA. Our results showed that the aperture of 7B-1 stomata does not increase in BL, suggesting that 7B-1 mutation impairs an element of BL signaling pathway involved in stomatal opening. Similar stomatal responses of 7B-1 and wild type (WT) to fusicoccin or 9-AC points out that activity of H(+)-ATPase and 9-AC-sensitive anion channels per se is not likely affected by the mutation. Since 9-AC restored stomatal opening of 7B-1 in BL, it seems that 9-AC and BL could block similar type of anion channels. The stomata of both genotypes did not respond to NIF neither in darkness nor in any light conditions tested. In light, 9-AC but not NIF restored stomatal opening inhibited by ABA in WT and 7B-1. We suggest that in comparison to WT, the activity of S-type anion channels in 7B-1 is more promoted by increased ABA content, and less reduced by BL, because of the mutant resistance to BL.
authors.affiliation: Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Biophysics, Faculty of Science, Palacký University in Olomouc, Šlechtitelů 11, Olomouc CZ-78371, Czech Republic. hlavinka.jan@gmail.com
authors.forename: Jan
authors.initials: J
authors.lastname: Hlavinka
authors.forename: Jan
authors.initials: J
authors.lastname: Nauš
authors.forename: Martin
authors.initials: M
authors.lastname: Fellner
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism* / Q000494:pharmacology; D000873:Anthracenes / Q000494:pharmacology; D018118:Chloride Channels / Q000037:antagonists & inhibitors; D003624:Darkness; D017343:Genes, Plant; D005838:Genotype; D008027:Light; D018551:Solanum lycopersicum / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D009154:Mutation; D010937:Plant Growth Regulators / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D054046:Plant Stomata / Q000187:drug effects / Q000502:physiology*; D006180:Proton-Translocating ATPases / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D000873:Anthracenes; D018118:Chloride Channels; D010937:Plant Growth Regulators; D010940:Plant Proteins; C010030:9-anthroic acid; D000040:Abscisic Acid; D006180:Proton-Translocating ATPases