title: Survivability of parthenogenetic embryos following in vivo transfer in naturally synchronized Capra hircus.
abstract: The present study was conducted to see the in vivo developmental potency of caprine parthenogenetic embryos generated in a modified way. The good quality caprine oocytes were matured in presence of cytochalasin B (CCB) and then activated by 7% ethanol followed by 2 mM 6-dimethyl amino purine (6-DMAP) and embryo development was recorded. Early stage parthenogenetic embryos (two to four cells) were surgically transferred in recipients (10). The pregnancy diagnosis was done by nonreturn to oestrus, ultrasonography (USG), and progesterone estimation. The levels of progesterone were above normal values (1 ng/ml) of pregnancy and fall below the level of pregnancy just before retuned to oestrus. Progesterone profile revealed that out of ten recipients (G1-G10), four goats (G1, G2, G3, and G5) returned to oestrus after 43 ± 7.29 (Mean ± SE) d of embryo transfer and six goats (G4, G6, G7, G8, G9, and G10) did not return to cycle even after 70 d of embryo transfer. In three recipients (G4, G5, and G6), the USG on day 40 revealed that there was fluid filled uterine body with solid fetus-like structure. These might be dead fetus and had started resorption. The progesterone profile also corroborated the assumption of pregnancy in these animals. Authors believe that this may be the first report on in vivo diploid parthenogenetic embryo development in caprine species.
authors.affiliation: Reproductive Physiology and Embryo Transfer Technology Laboratory, Physiology and Climatology Division, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243 122, India. dr_raviranjan@yahoo.co.in
authors.forename: Ravi
authors.initials: R
authors.lastname: Ranjan
authors.forename: Rakesh Kumar
authors.initials: RK
authors.lastname: Singh
authors.forename: Thirupathi
authors.initials: T
authors.lastname: Yasotha
authors.forename: Manish
authors.initials: M
authors.lastname: Kumar
authors.forename: Kuldeep
authors.initials: K
authors.lastname: Kumar
authors.forename: Renu
authors.initials: R
authors.lastname: Singh
authors.forename: Monzamul
authors.initials: M
authors.lastname: Houque
authors.forename: Vijay Prakash
authors.initials: VP
authors.lastname: Mourya
authors.forename: Gyanendra
authors.initials: G
authors.lastname: Singh
authors.forename: Mihir
authors.initials: M
authors.lastname: Sarkar
authors.forename: Bikash Chandra
authors.initials: BC
authors.lastname: Das
authors.forename: Sadhan
authors.initials: S
authors.lastname: Bag
mesh_terms: D000818:Animals; D004624:Embryo Transfer; D004622:Embryo, Mammalian / Q000502:physiology*; D005260:Female; D006041:Goats / Q000196:embryology* / Q000502:physiology; D009865:Oocytes / Q000166:cytology / Q000378:metabolism; D010312:Parthenogenesis; D011247:Pregnancy; D011374:Progesterone / Q000378:metabolism
chemical_list: D011374:Progesterone