title: Effects of acute ozone exposure on lung peak allergic inflammation of mice.
abstract: Asthma exacerbations are often triggered by air pollution, including O3, whereas how patients with asthma exacerbations react to high levels of ambient ozone remain unknown. Here, we investigated the manner in which acute ozone exposure affects the pathophysiological characteristics of an asthma model on the premise of culminated allergic airway inflammation. The asthma model was constructed in mice, and enhanced pause (Penh), total and differential cell number, soluble mediator concentration, histopathology, and Muc5ac mRNA expression in the mice were observed. The results showed that ozone could induce airway hyperresponsiveness (AHR) in controls and an additional enhancement of preexisting AHR in asthmatic mice. When exposed to ozone, the asthmatic mice expressed more neutrophils, TNF-α, IL-13, and hyaluronan in bronchoalveolar lavage than controls. The mice with asthma and the controls both showed decreased epithelial cell density in the proximal and distal airways. Ozone aggravated the increased mucus production and mucin gene expression in mice with asthma. These results show that subjects with asthma may react differently to the same high level of ambient ozone, especially for those with asthma exacerbations.
authors.affiliation: Department of Respiratory Medicine, The Affiliated First People's Hospital of Shanghai Jiaotong University, Shanghai 200080, China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Bao
authors.forename: Li
authors.initials: L
authors.lastname: Liang
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001483:Base Sequence; D017931:DNA Primers; D005260:Female; D006967:Hypersensitivity / Q000209:etiology*; D008168:Lung / Q000187:drug effects*; D051379:Mice; D008807:Mice, Inbred BALB C; D055271:Mucin 5AC / Q000235:genetics; D010126:Ozone / Q000633:toxicity*; D011014:Pneumonia / Q000139:chemically induced*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D055271:Mucin 5AC; D012333:RNA, Messenger; D010126:Ozone