title: Integrated proteomic analysis of post-translational modifications by serial enrichment.
abstract: We report a mass spectrometry-based method for the integrated analysis of protein expression, phosphorylation, ubiquitination and acetylation by serial enrichments of different post-translational modifications (SEPTM) from the same biological sample. This technology enabled quantitative analysis of nearly 8,000 proteins and more than 20,000 phosphorylation, 15,000 ubiquitination and 3,000 acetylation sites per experiment, generating a holistic view of cellular signal transduction pathways as exemplified by analysis of bortezomib-treated human leukemia cells.
authors.affiliation: The Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA. pmertins@broadinstitute.org
authors.forename: Philipp
authors.initials: P
authors.lastname: Mertins
authors.forename: Jana W
authors.initials: JW
authors.lastname: Qiao
authors.forename: Jinal
authors.initials: J
authors.lastname: Patel
authors.forename: Namrata D
authors.initials: ND
authors.lastname: Udeshi
authors.forename: Karl R
authors.initials: KR
authors.lastname: Clauser
authors.forename: D R
authors.initials: DR
authors.lastname: Mani
authors.forename: Michael W
authors.initials: MW
authors.lastname: Burgess
authors.forename: Michael A
authors.initials: MA
authors.lastname: Gillette
authors.forename: Jacob D
authors.initials: JD
authors.lastname: Jaffe
authors.forename: Steven A
authors.initials: SA
authors.lastname: Carr
mesh_terms: D020869:Gene Expression Profiling / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D011499:Protein Processing, Post-Translational / Q000502:physiology*; D020543:Proteome / Q000378:metabolism*; D018511:Systems Integration
chemical_list: D020543:Proteome