title: αCaMKII autophosphorylation controls the establishment of alcohol-induced conditioned place preference in mice.
abstract: The autophosphorylation of alpha Ca2+ /calmodulin dependent protein kinase II (αCaMKII) is important for memory formation and is becoming increasingly implicated in the development of drug addiction. Previous work suggests that αCaMKII acts via the monoaminergic systems to facilitate the establishment of alcohol drinking behaviour. The present study aims to investigate whether αCaMKII autophosphorylation deficient αCaMKII(T286A) mice show a difference in the rewarding properties of alcohol (2 g/kg, i.p.), as measured by conditioned place preference (CPP). We found that alcohol-induced CPP could be established at an accelerated rate in αCaMKII(T286A) compared to wild type (WT) mice. Hyperactivity/hyper-arousal induced by the test environment was normalised by alcohol in the αCaMKII(T286A), but not WT mice. This effect could be conditioned to the test environment and may suggest enhanced negative reinforcing action of alcohol in αCaMKII autophosphorylation deficient mice.
authors.affiliation: MRC Social, Genetic and Developmental Psychiatry Research Centre, Institute of Psychiatry, King's College London, De Crespigny Park, London SE5 8AF, UK.
authors.forename: Alanna C
authors.initials: AC
authors.lastname: Easton
authors.forename: Walter
authors.initials: W
authors.lastname: Lucchesi
authors.forename: Keiko
authors.initials: K
authors.lastname: Mizuno
authors.forename: Cathy
authors.initials: C
authors.lastname: Fernandes
authors.forename: Gunter
authors.initials: G
authors.lastname: Schumann
authors.forename: K Peter
authors.initials: KP
authors.lastname: Giese
authors.forename: Christian P
authors.initials: CP
authors.lastname: Müller
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D016739:Behavior, Addictive / Q000503:physiopathology; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2 / Q000172:deficiency / Q000378:metabolism*; D002492:Central Nervous System Depressants / Q000494:pharmacology*; D003216:Conditioning, Operant / Q000187:drug effects*; D000431:Ethanol / Q000494:pharmacology*; D005108:Extinction, Psychological / Q000187:drug effects; D005260:Female; D008124:Locomotion / Q000187:drug effects / Q000235:genetics; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D010766:Phosphorylation
chemical_list: D002492:Central Nervous System Depressants; D000431:Ethanol; D054732:Calcium-Calmodulin-Dependent Protein Kinase Type 2
keywords: Alcohol; AlphaCaMKII; Autophosphorylation; Conditioned place preference; Locomotion; Reinforcement