title: Functional analysis of the gene SCO1782 encoding Streptomyces hemolysin (S-hemolysin) in Streptomyces coelicolor M145.
abstract: In the process of evaluating the growth of Streptomyces coelicolor on rich media such as blood agar, we found that S. coelicolor a non-pathogenic, well-known antibiotic producer had the ability to grow and produce a prominent hemolytic zone. By comparing the growth with an agarase gene mutant of S. coelicolor, a similar prominent hemolytic zone was found to develop due to the organism's hemolytic activity. After the confirmation of hemolytic activity from S. coelicolor, the genome was searched for hemolysin-coding genes; consequently, SCO1782, SCO2534, and SCO3882 were identified, whose products were annotated as a putative, membrane, and hypothetical proteins, respectively. Functional characterization of all the recombinant proteins expressed in Escherichia coli BL21(DE3) revealed that only SCO1782 exhibited hemolytic activity. This S. coelicolor protein, designated as S-hemolysin, showed sequence similarity toward hemolysins from Brachyspira hyodysenteriae (35%) and Mycobacterium tuberculosis (62%). Recombinant hemolysin exhibited activity against sheep blood erythrocytes and cytolytic activity against human fibroblast cells. Deletion of SCO1782 resulted in complete loss of hemolysin activity in S. coelicolor.
authors.affiliation: Department of Microbial Engineering, College of Engineering, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
authors.forename: Thangamani
authors.initials: T
authors.lastname: Rajesh
authors.forename: Jong-Min
authors.initials: JM
authors.lastname: Jeon
authors.forename: Yong-Hyun
authors.initials: YH
authors.lastname: Kim
authors.forename: Hyun-Joong
authors.initials: HJ
authors.lastname: Kim
authors.forename: Da Hye
authors.initials: da H
authors.lastname: Yi
authors.forename: Sung-Hee
authors.initials: SH
authors.lastname: Park
authors.forename: Kwon-Young
authors.initials: KY
authors.lastname: Choi
authors.forename: Yun-Gon
authors.initials: YG
authors.lastname: Kim
authors.forename: Jaebum
authors.initials: J
authors.lastname: Kim
authors.forename: Seunho
authors.initials: S
authors.lastname: Jung
authors.forename: Hyung-Yeon
authors.initials: HY
authors.lastname: Park
authors.forename: Yung-Hun
authors.initials: YH
authors.lastname: Yang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D003470:Culture Media; D004912:Erythrocytes / Q000187:drug effects / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D017353:Gene Deletion; D005798:Genes, Bacterial; D006460:Hemolysin Proteins / Q000737:chemistry / Q000235:genetics*; D053878:Hemolytic Agents / Q000494:pharmacology; D006801:Humans; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D012756:Sheep; D048372:Streptomyces coelicolor / Q000737:chemistry / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D003470:Culture Media; D006460:Hemolysin Proteins; D053878:Hemolytic Agents; D011994:Recombinant Proteins
keywords: Cytolytic activity; Hemolytic activity; S-hemolysin; Streptomyces coelicolor