title: A conserved tyrosine in ferritin is a molecular capacitor.
abstract: A highly conserved tyrosine residue of unknown function is present in the vicinity of the di-iron catalytic center of the ubiquitous iron-storage protein ferritin. The di-iron center with a gateway FeII/FeIII-binding site nearby provides the vital iron-storage mechanism of the protein. It is believed that, in eukaryotic ferritin, this center catalyzes simultaneous oxidation of two FeII ions, whereas in microbial ferritin it catalyzes simultaneous oxidation of three FeII ions. To understand the role of the conserved tyrosine, we studied the intermediates and products that are formed during catalysis of FeII oxidation in the di-iron catalytic centers of the hyperthermophilic archaeal Pyrococcus furiosus ferritin and of eukaryotic human H ferritin. Based on our spectroscopic studies and modeling, we propose a merger of the models for eukaryotic and bacterial ferritin into a common mechanism of FeII oxidation in which the conserved tyrosine acts as a single-electron molecular capacitor to facilitate oxidation of FeII.
authors.affiliation: Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628BC, Delft, The Netherlands.
authors.forename: Kourosh Honarmand
authors.initials: KH
authors.lastname: Ebrahimi
authors.forename: Peter-Leon
authors.initials: PL
authors.lastname: Hagedoorn
authors.forename: Wilfred R
authors.initials: WR
authors.lastname: Hagen
mesh_terms: D020134:Catalytic Domain; D005293:Ferritins / Q000737:chemistry* / Q000378:metabolism; D005296:Ferrous Compounds / Q000737:chemistry; D007700:Kinetics; D010084:Oxidation-Reduction; D019711:Pyrococcus / Q000378:metabolism; D014443:Tyrosine / Q000737:chemistry*
chemical_list: D005296:Ferrous Compounds; D014443:Tyrosine; D005293:Ferritins