title: Increased levels of plasma soluble Sema4D in patients with heart failure.
abstract: Semaphorin 4D (Sema4D/CD100) is a 150-kDa transmembrane glycoprotein expressed by platelets and T-cells. When these cells are activated, Sema4D is cleaved proteolytically, generating a biologically active 120-kDa fragment (soluble Sema4D) capable of targeting receptors on platelets, B-cells, endothelial cells and tumor cells. However, its plasma levels and significance in heart failure (HF) have not been reported. In this study, we established an ELISA and detected soluble Sema4D in human plasma. In healthy controls, plasma Sema4D levels were higher in men than women (5.15±3.30 ng/mL, n = 63, vs. 4.19±2.39 ng/mL, n = 63, P<0.05). In HF patients, plasma Sema4D levels were significantly higher than those in healthy controls (8.94±5.89 ng/mL, n = 157 vs. 4.67±2.99 ng/mL, n = 126, P<0.0001) with the highest levels being in HF patients with diabetes mellitus (DM) (10.45±5.76 ng/mL, n = 40). We also found that there was a higher percentage of Sema4D(high) CD3(+) (P<0.01), CD4(+) (P<0.001), and CD8(+) (P<0.01) T-cells in samples from HF patients, but no changes in Sema4D expression levels in B cells and platelets. Therefore, our investigation shows that plasma Sema4D levels are increased in HF patients, especially in those who also have diabetes. There was an accompanying increase in the Sema4D(high) population of T-cells, suggesting a potential role of these T-cells in heart failure.
authors.affiliation: Cyrus Tang Hematology Center, Soochow University, Suzhou, Jiangsu, China.
authors.forename: Qiongyu
authors.initials: Q
authors.lastname: Lu
authors.forename: Ningzheng
authors.initials: N
authors.lastname: Dong
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.forename: Wenxiu
authors.initials: W
authors.lastname: Yi
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Wang
authors.forename: Shengjie
authors.initials: S
authors.lastname: Zhang
authors.forename: Haibo
authors.initials: H
authors.lastname: Gu
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Tang
authors.forename: Boquan
authors.initials: B
authors.lastname: Jin
authors.forename: Qingyu
authors.initials: Q
authors.lastname: Wu
authors.forename: Lawrence F
authors.initials: LF
authors.lastname: Brass
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
mesh_terms: D000328:Adult; D000818:Animals; D015703:Antigens, CD / Q000097:blood* / Q000235:genetics / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology / Q000473:pathology; D017252:CD3 Complex / Q000235:genetics / Q000276:immunology; D015704:CD4 Antigens / Q000235:genetics / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D016022:Case-Control Studies; D048909:Diabetes Complications; D003920:Diabetes Mellitus / Q000097:blood* / Q000276:immunology / Q000473:pathology; D005260:Female; D015870:Gene Expression; D006333:Heart Failure / Q000097:blood* / Q000150:complications / Q000276:immunology / Q000473:pathology; D006801:Humans; D018655:Lymphocyte Count; D008297:Male; D008875:Middle Aged; D039961:Semaphorins / Q000097:blood* / Q000235:genetics / Q000276:immunology; D012737:Sex Factors
chemical_list: D015703:Antigens, CD; C093224:CD100 antigen; D017252:CD3 Complex; D015704:CD4 Antigens; D039961:Semaphorins