title: [Study on MEKC-DAD fingerprint of Scutellariae radix].
abstract: OBJECTIVE
  To establish the analytical method for the fingerprint of Scutellariae Radix by MEKC-DAD and compare the fingerprints of Scutellariae Radix, Scutellariae Radix Stir-baked and Scutellariae Radix Green.
  
  
  METHODS
  Based on the mode of micellar electrokinetic chromatography, 40 mmol/L sodium hydrogen phosphate, 15 mmol/L sodium borate, 40 mmol/L SDS, 15% acetonitrile, 7.5% propyl alcohol were selected for the running buffer (pH 8.4). The separation voltage was 20 kV and the detection wavelength was set at 280 nm. Baicalin was used as reference standard, the chromatographic fingerprint was established.
  
  
  RESULTS
  MEKC-DAD fingerprint with 9 main peaks was established preliminarily. Regarding to the fingerprints of Scutellariae Radix and its processed products, the samples before and after storage moisture, there were obvious differences in the relative areas of common peaks.
  
  
  CONCLUSIONS
  The method is reliable, accurate and can be used for quality control of Scutellariae Radix.
authors.affiliation: Jiangsu Key Laboratory of Chinese Medicines Processing, Nanjing University of Chinese Medicine, Nangjing 210046, China. winner2243@sohu.com
authors.forename: Le
authors.initials: L
authors.lastname: Han
authors.forename: Qiao-Xia
authors.initials: QX
authors.lastname: Chen
authors.forename: Xun-Hong
authors.initials: XH
authors.lastname: Liu
authors.forename: Yi-Zhi
authors.initials: YZ
authors.lastname: Zhou
authors.forename: Jun-Song
authors.initials: JS
authors.lastname: Li
authors.forename: Bao-Chang
authors.initials: BC
authors.lastname: Cai
mesh_terms: D001881:Borates / Q000737:chemistry; D002021:Buffers; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000592:standards; D018517:Plant Roots / Q000737:chemistry; D011786:Quality Control; D015203:Reproducibility of Results; D031364:Scutellaria baicalensis / Q000737:chemistry*
chemical_list: D001881:Borates; D002021:Buffers; D004365:Drugs, Chinese Herbal