title: Radiation-inducible protein RbAp48 contributes to radiosensitivity of cervical cancer cells.
abstract: OBJECTIVE
  Retinoblastoma-associated protein 48 (RbAp48) has been recently discovered as a radiosensitive gene. We aimed to investigate the role of RbAp48 in radiosensitivity of cervical cancer cells in vivo and in vitro.
  
  
  METHODS
  We used real-time RT-PCR and Western blot assay to examine the expression of RbAp48 in irradiated cervical cancer cell lines, including SiHa, Caski, and HeLa cells. The role of RbAp48 in radiosensitivity of cervical cancer cells was assessed by cell proliferation, counting, survival, and apoptosis as well as cell cycle and tumor growth assays with RbAp48 overexpression or gene silencing.
  
  
  RESULTS
  The expression of RbAp48 was increased in irradiated cervical cancer cell lines. Overexpression of RbAp48 induced G2/M arrest and apoptosis in irradiated cells, which was related to upregulation of p53, Rb and caspase-8 expression. Adenovirus-RbAp48 infection and irradiation synergistically inhibited tumor growth in nude mice.
  
  
  CONCLUSIONS
  RbAp48 is a radiation-inducible gene in cervical cancer cells because of enhanced radiosensitivity of cervical cancer cells in vivo and in vitro. RbAp48 may be a potential target to improve the results of radiation therapy for patients with cervical cancer.
authors.affiliation: Department of Medical Microbiology, Shandong University School of Medicine, Jinan, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zheng
authors.forename: Wei
authors.initials: W
authors.lastname: Tang
authors.forename: Fanhua
authors.initials: F
authors.lastname: Wei
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.forename: Juan
authors.initials: J
authors.lastname: Liu
authors.forename: Yi
authors.initials: Y
authors.lastname: Lu
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Cheng
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Bai
authors.forename: Xiuping
authors.initials: X
authors.lastname: Yu
authors.forename: Weiming
authors.initials: W
authors.lastname: Zhao
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D053181:Caspase 8 / Q000235:genetics; D049109:Cell Proliferation / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D005260:Female; D059565:G2 Phase Cell Cycle Checkpoints; D015870:Gene Expression / Q000528:radiation effects*; D020868:Gene Silencing; D006367:HeLa Cells; D006801:Humans; D059566:M Phase Cell Cycle Checkpoints; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011836:Radiation Tolerance; D016160:Retinoblastoma Protein / Q000235:genetics; D056505:Retinoblastoma-Binding Protein 4 / Q000235:genetics* / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000532:radiotherapy
chemical_list: D016160:Retinoblastoma Protein; D056505:Retinoblastoma-Binding Protein 4; D016159:Tumor Suppressor Protein p53; D053181:Caspase 8
keywords: Cervical cancer; Radiation therapy; Radiosensitivity; RbAp48