title: Genetic mapping of agenesis of the third molars in mice.
abstract: EL/Sea mice are characterized by 100% incidence of agenesis of the third molars (M3). In a previous study, chromosomal mapping of the ninth generation EL/Sea congenic strains revealed a major locus for agenesis of M3, designated am3, in the 125-137 Mbp region of chromosome 3. In the present study, to determine the precise location of the am3 locus, we produced further generations of the EL/Sea congenic strains for am3 in which the restricted interval on chromosome 3 of EL/Sea was replaced by a MSM/Msf-derived homolog. The eleventh generation congenic mice that were either heterozygous or homozygous for the MSM/Msf-derived interval exhibited a significant decrease in the incidence of M3 agenesis (p < 0.00001). Results confined the am3 locus to an interval of 1 Mbp on chromosome 3, demonstrating that Lef1, one of the essential transcription factors for early tooth development, is the strongest candidate for am3.
authors.affiliation: Department of Pediatric Dentistry, Nihon University School of Dentistry at Matsudo, 2-870-1 Sakaecho-Nishi, Matsudo, Chiba, 271-0061, Japan, shimizu.takehiko@nihon-u.ac.jp.
authors.forename: Takehiko
authors.initials: T
authors.lastname: Shimizu
authors.forename: Wataru
authors.initials: W
authors.lastname: Morita
authors.forename: Takahide
authors.initials: T
authors.lastname: Maeda
mesh_terms: D000818:Animals; D000848:Anodontia / Q000235:genetics*; D002874:Chromosome Mapping; D033481:Chromosomes, Mammalian / Q000235:genetics; D004252:DNA Mutational Analysis; D004195:Disease Models, Animal; D005260:Female; D008040:Genetic Linkage; D020022:Genetic Predisposition to Disease; D005838:Genotype; D051821:Lymphoid Enhancer-Binding Factor 1 / Q000235:genetics*; D008297:Male; D051379:Mice; D020297:Mice, Congenic; D008964:Molar, Third / Q000002:abnormalities*
chemical_list: C497574:Lef1 protein, mouse; D051821:Lymphoid Enhancer-Binding Factor 1