title: Oxidative biomarkers to assess the nanoparticle-induced oxidative stress.
abstract: Nanotechnology involves the creation and manipulation of materials at nanoscale levels to create products that exhibit novel properties. Engineered nanomaterials either metals (like carbon and silver) or metal oxides (like zinc oxide, magnesium oxide, and titanium oxide) induce toxicity and oxidative stress by generating free radicals. Various in vitro and in vivo models are available to estimate the oxidative stress induced by the nanoparticles. In this chapter, we describe the methods for the estimation of oxidative stress markers like reactive oxygen species (ROS), DNA damage estimation, and lipid peroxidation products; total antioxidant capacity (TAC) was mentioned.
authors.affiliation: Department of Pharmacology, Vaageswari College of Pharmacy, Karimnagar, Andhra Pradesh, India.
authors.forename: Rama Narsimha Reddy
authors.initials: RN
authors.lastname: Anreddy
authors.forename: Narsimha Reddy
authors.initials: NR
authors.lastname: Yellu
authors.forename: Krishna R
authors.initials: KR
authors.lastname: Devarakonda
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002478:Cells, Cultured; D004249:DNA Damage; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation; D053768:Metal Nanoparticles / Q000633:toxicity*; D015336:Molecular Probe Techniques; D018384:Oxidative Stress; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D005456:Fluorescent Dyes; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D005978:Glutathione