title: 3D-fection: cell transfection within 3D scaffolds and hydrogels.
abstract: BACKGROUND
  3D matrices are widely used as cell growth supports in basic research, regenerative medicine or cell-based drug assays. In order to genetically manipulate cells cultured within 3D matrices, two novel non-viral transfection reagents allowing preparation of matrices for in situ cell transfection were evaluated.
  
  
  RESULTS
  Two lipidic formulations, 3D-Fect™ and 3D-FectIN™, were assessed for their ability to transfect cells cultured within 3D solid scaffolds and 3D hydrogels, respectively. These reagents showed good compatibility with the most widespread types of matrices and enabled transfection of a wide range of mammalian cells of various origins. Classical cell lines, primary cells and stem cells were thus genetically modified while colonizing their growth support. Importantly, this in situ strategy alleviated the need to manipulate cells before seeding them.
  
  
  CONCLUSIONS
  Results presented here demonstrated that 3D-Fect and 3D-FectIN reagents for 3D transfection are totally compatible with cells and do not impair matrix properties. 3D-Fect and 3D-FectIN, therefore, provide valuable tools for achieving localized and sustained transgene expression and should find versatile applications in fundamental research, regenerative medicine and cell-based drug assays.
authors.affiliation: Parc Scientifique de Luminy - Zone Entreprise, Marseille cedex 9, France. nlaurent@ozbiosciences.com
authors.forename: Cédric
authors.initials: C
authors.lastname: Sapet
authors.forename: Cécile
authors.initials: C
authors.lastname: Formosa
authors.forename: Flavie
authors.initials: F
authors.lastname: Sicard
authors.forename: Elodie
authors.initials: E
authors.lastname: Bertosio
authors.forename: Olivier
authors.initials: O
authors.lastname: Zelphati
authors.forename: Nicolas
authors.initials: N
authors.lastname: Laurent
mesh_terms: D000818:Animals; D020868:Gene Silencing; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D008856:Microscopy, Fluorescence; D054457:Tissue Scaffolds; D014162:Transfection / Q000379:methods*; D019076:Transgenes
chemical_list: D020100:Hydrogels