title: Immobilization studies of an engineered arginine-tryptophan-rich peptide on a silicone surface with antimicrobial and antibiofilm activity.
abstract: With the rapid rise of antibiotic-resistant-device-associated infections, there has been increasing demand for an antimicrobial biomedical surface. Synthetic antimicrobial peptides that have excellent bactericidal potency and negligible cytotoxicity are promising targets for immobilization on these target surfaces. An engineered arginine-tryptophan-rich peptide (CWR11) was developed, which displayed potent antimicrobial activity against a broad spectrum of microbes via membrane disruption, and possessed excellent salt resistance properties. A tethering platform was subsequently developed to tether CWR11 onto a model polymethylsiloxane (PDMS) surface using a simple and robust strategy. Surface characterization assays such as attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), X-ray photoelectron spectroscopy (XPS), and energy-dispersive X-ray spectroscopy (EDX) confirmed the successful grafting of CWR11 onto the chemically treated PDMS surface. The immobilized peptide concentration was 0.8 ± 0.2 μg/cm(2) as quantitated by sulfosuccinimidyl-4-o-(4,4-dimethoxytrityl) butyrate (sulfo-SDTB) assay. Antimicrobial assay and cytotoxic investigation confirmed that the peptide-immobilized surface has good bactericidal and antibiofilm properties, and is also noncytotoxic to mammalian cells. Tryptophan-arginine-rich antimicrobial peptides have the potential for antimicrobial protection of biomedical surfaces and may have important clinical applications in patients.
authors.affiliation: School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore.
authors.forename: Kaiyang
authors.initials: K
authors.lastname: Lim
authors.forename: Ray Rong Yuan
authors.initials: RR
authors.lastname: Chua
authors.forename: Rathi
authors.initials: R
authors.lastname: Saravanan
authors.forename: Anindya
authors.initials: A
authors.lastname: Basu
authors.forename: Biswajit
authors.initials: B
authors.lastname: Mishra
authors.forename: Paul Anantharajah
authors.initials: PA
authors.lastname: Tambyah
authors.forename: Bow
authors.initials: B
authors.lastname: Ho
authors.forename: Susanna Su Jan
authors.initials: SS
authors.lastname: Leong
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D001120:Arginine / Q000737:chemistry* / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects*; D002460:Cell Line; D004926:Escherichia coli / Q000187:drug effects / Q000502:physiology; D006801:Humans; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000502:physiology; D012828:Silicones / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects / Q000502:physiology; D014364:Tryptophan / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D010455:Peptides; D012828:Silicones; D014364:Tryptophan; D001120:Arginine