title: Genotypes of Candida albicans isolated from healthy individuals and their distribution in patients with oral candidiasis.
abstract: For the study of Candida albicans genotypes involved in development of candidiasis, Candida albicans isolates were collected from healthy volunteers and patients with oral candidiasis and genotyped on the basis of 25S rDNA and microsatellite polymorphisms. In the microsatellite analysis using two microsatellite markers (CDC3 and CAI), 63 healthy volunteer isolates were classified into 35 genotypes (allelic relations to CDC3 alleles 1:2/CAI alleles 1:2), among which genotypes II (115:119/23:23), III (115:123/18:27), and V (123:127/32:41) were found at frequencies of 12.7%, 7.9%, and 7.9%, respectively. In 68 oral candidiasis isolates classified into 39 genotypes, genotypes II and III were identified in 4.4% and 20.6% of the isolates, respectively. The frequency of genotype III was higher in the candidiasis isolates than in the healthy isolates (p < 0.05). These results suggest that genotype III C. albicans assigned by CDC3/CAI is related to the development of oral candidiasis.
authors.affiliation: Department of Maxillofacial Surgery, Aichi-Gakuin University School of Dentistry, Nagoya, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Takagi
authors.forename: Hideo
authors.initials: H
authors.lastname: Fukano
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shimozato
authors.forename: Reiko
authors.initials: R
authors.lastname: Tanaka
authors.forename: Toshinobu
authors.initials: T
authors.lastname: Horii
authors.forename: Fumihiko
authors.initials: F
authors.lastname: Kawamoto
authors.forename: Toshio
authors.initials: T
authors.lastname: Kanbe
mesh_terms: D000328:Adult; D000368:Aged; D002176:Candida albicans / Q000235:genetics* / Q000302:isolation & purification; D002180:Candidiasis, Oral / Q000382:microbiology*; D002353:Carrier State / Q000382:microbiology*; D016022:Case-Control Studies; D004271:DNA, Fungal / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male
chemical_list: D004271:DNA, Fungal