title: [Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus field strains in central China during 2010-2012 outbreaks].
abstract: Since late 2010, porcine epidemic diarrhea virus (PEDV) has been re-emerging in central China. To explore the possible reason of the PEDV outbreaks, twelve PEDV field strains were isolated from different swine breeding farms in central China during 2010-2012, and molecular diversity, phylogenetic relationships of these strains with other PEDV reference strains were investigated. Sequence analysis of S, M and ORE3 genes revealed that the central China PEDV isolates had several specific nucleotides and amino acids which were different from PEDV reference strains. In addition, the entire S genes of eleven central China PEDV isolates were found to be nine nucleotides longer in length than CV777 and large number of amino acid variations was accumulated in the N-terminal region of S gene. Phylogenetic analysis showed that the central China PEDV isolates had close relationship with Korea strains (2007-2009), Thailand strains (2007-2008), Vietnam strains (2009-2010), Japan strains (2010), and other prevailing strains from other parts of China (2010-2012). However, they differed genetically from European strains (CV777, Brl/87), China strains (2003-2007) and the vaccine strains (CV777) used in China. These results imply that a rapid variation and evolution of central China PEDV strains has occurred in recent years, and a more efficient vaccine strain should be selected to prevent and control outbreaks of PEDV in China.
authors.affiliation: Henan Agricultural University, Zhengzhou 450002, China. zhengfengmei163@163.com
authors.forename: Feng-Mei
authors.initials: FM
authors.lastname: Zheng
authors.forename: Jin-Yao
authors.initials: JY
authors.lastname: Huo
authors.forename: Jun
authors.initials: J
authors.lastname: Zhao
authors.forename: Hong-Tao
authors.initials: HT
authors.lastname: Chang
authors.forename: Xiao-Meng
authors.initials: XM
authors.lastname: Wang
authors.forename: Lu
authors.initials: L
authors.lastname: Chen
authors.forename: Chuan-Qing
authors.initials: CQ
authors.lastname: Wang
mesh_terms: D000818:Animals; D002681:China / Q000453:epidemiology; D004196:Disease Outbreaks; D005243:Feces / Q000821:virology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D053485:Porcine epidemic diarrhea virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins