title: Quantification of tumor necrosis factor-α and matrix metalloproteinases-3 in synovial fluid by a fiber-optic particle plasmon resonance sensor.
abstract: The availability of techniques for sensitive detection of early stage osteoarthritis is critical for improving patient health. This study illustrates the feasibility of a fiber-optic particle plasmon resonance (FOPPR) sensor with gold nanoparticles on the unclad region of optical fiber probes for analysis of osteoarthritis biomarkers, tumor necrosis factor-α (TNF-α) and matrix metalloproteinases-3 (MMP-3). Results show that the sensor can achieve a refractive index resolution of 5.18 × 10⁻⁷ RIU and limits of detection for TNF-α and MMP-3 as low as 8.22 pg ml⁻¹ (0.48 pM) and 34.3 pg ml⁻¹ (1.56 pM), respectively. Additionally, the FOPPR sensor shows a good correlation in determining TNF-α and MMP-3 in synovial fluid with the clinically accepted enzyme-linked immunosorbent assay (ELISA) method. Finally, given the FOPPR sensor's nature of being low-cost, label-free, highly sensitive, real-time, simple-to-operate, the FOPPR sensor could offer potential to monitor biomarkers of various diseases, and provide an ideal technical tool for point-of-care diagnostics.
authors.affiliation: Department of Chemistry and Biochemistry, National Chung Cheng University, Chiayi, 621, Taiwan.
authors.forename: Yi-Ching
authors.initials: YC
authors.lastname: Huang
authors.forename: Chang-Yue
authors.initials: CY
authors.lastname: Chiang
authors.forename: Cheng-Hen
authors.initials: CH
authors.lastname: Li
authors.forename: Ting-Chou
authors.initials: TC
authors.lastname: Chang
authors.forename: Chung-Sheng
authors.initials: CS
authors.lastname: Chiang
authors.forename: Lai-Kwan
authors.initials: LK
authors.lastname: Chau
authors.forename: Kuo-Wei
authors.initials: KW
authors.lastname: Huang
authors.forename: Chin-Wei
authors.initials: CW
authors.lastname: Wu
authors.forename: Shau-Chun
authors.initials: SC
authors.lastname: Wang
authors.forename: Shaw-Ruey
authors.initials: SR
authors.lastname: Lyu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D005240:Feasibility Studies; D006801:Humans; D019278:Matrix Metalloproteinase 3 / Q000032:analysis*; D055100:Optical Fibers; D010003:Osteoarthritis / Q000175:diagnosis; D020349:Surface Plasmon Resonance / Q000379:methods*; D013582:Synovial Fluid / Q000737:chemistry*; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis*
chemical_list: D014409:Tumor Necrosis Factor-alpha; C511768:MMP3 protein, human; D019278:Matrix Metalloproteinase 3