title: Molecular fingerprinting of bacterial communities in enriched azo dye (Reactive Violet 5R) decolorising native acclimatised bacterial consortia.
abstract: Reactive Violet 5R (RV5R) decolorising acclimatised bacterial consortia were enriched from industrial effluent contaminated and pristine samples from Gujarat, India on several different media. Twelve acclimatised consortia were selected for the study which were able to decolorise 100mg/L RV5R in 30 h under shaking or static conditions. Eubacterial diversity was studied by 16S rRNA gene based culture-independent methods, using HaeIII and Hinf1 enzymes for ARDRA and V3 region based DGGE analysis, forming total 6 clusters in both analysis. Decolorised end products of all the consortia were analysed by FTIR showing cleavage of the azo bond and group modifications. GC-MS data of dye decolorised end products of Gly consortium obtained from hydrocarbon contaminated soil demonstrated benzene ring cleavage activity. Present study suggests that enrichment of acclimatised consortia under different conditions can result in diverse microbial communities that differentially degrade RV5R and can provide rich source of dye decolorising strains.
authors.affiliation: Department of Microbiology and Biotechnology Centre, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara 390 002, Gujarat, India.
authors.forename: Jagat
authors.initials: J
authors.lastname: Rathod
authors.forename: G
authors.initials: G
authors.lastname: Archana
mesh_terms: D001391:Azo Compounds / Q000378:metabolism*; D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003116:Color; D004396:Coloring Agents / Q000378:metabolism*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D008401:Gas Chromatography-Mass Spectrometry; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D001391:Azo Compounds; D004396:Coloring Agents; D017931:DNA Primers; D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: Acclimatised bacterial consortia; Amplified ribosomal DNA restriction digestion analysis (ARDRA); Azo dye decolorisation; Denaturing gradient gel electrophoresis (DGGE); Fourier transformed infrared spectroscopy (FTIR)