title: Effects of single exposure of sodium fluoride on lipid peroxidation and antioxidant enzymes in salivary glands of rats.
abstract: Many studies suggest that fluoride exposure can inhibit the activity of various enzymes and can generate free radicals, which interfere with antioxidant defence mechanisms in living systems. To further the understanding of this issue, this present study examined the effects of low-dose fluoride treatment on the activity of enzymatic antioxidant superoxide dismutase (SOD) and catalase (CAT), as well as the levels of lipid peroxidation (LPO) in the parotid (PA) and submandibular (SM) salivary glands of rats. Rats were injected with a single dose of sodium fluoride (NaF) (15 mg F(-)/kg b.w.) then euthanized at various time intervals up to 24 hours (h) following exposure. NaF exposure did not cause significant differences in SOD or CAT activity or LPO levels in PA glands compared to control. Conversely, SM glands presented increased SOD activity after 3 h and decreased SOD activity after 1, 12, and 24 h, while LPO was increased after 6, 12, and 24 h of the NaF injection. There were no significant differences in the CAT activity in the groups studied. Our results demonstrated that NaF intoxication caused oxidative stress in salivary glands few hours after administration. These changes were more pronounced in SM than in PA gland.
authors.affiliation: Departamento de Biomateriais e Biologia Oral, Faculdade de Odontologia, Universidade de São Paulo, 05508-000 São Paulo, SP, Brazil.
authors.forename: Paula Mochidome
authors.initials: PM
authors.lastname: Yamaguti
authors.forename: Alyne
authors.initials: A
authors.lastname: Simões
authors.forename: Emily
authors.initials: E
authors.lastname: Ganzerla
authors.forename: Douglas Nesadal
authors.initials: DN
authors.lastname: Souza
authors.forename: Fernando Neves
authors.initials: FN
authors.lastname: Nogueira
authors.forename: José
authors.initials: J
authors.lastname: Nicolau
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010306:Parotid Gland / Q000187:drug effects / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D012469:Salivary Glands / Q000187:drug effects / Q000201:enzymology*; D012969:Sodium Fluoride / Q000494:pharmacology*; D013363:Submandibular Gland / Q000187:drug effects / Q000201:enzymology; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D008315:Malondialdehyde; D012969:Sodium Fluoride; D002374:Catalase; D013482:Superoxide Dismutase