title: Attenuation of lithium-induced natriuresis and kaliuresis in P2Y₂ receptor knockout mice.
abstract: Whole body knockout (KO) of the P2Y₂ receptor (P2Y₂R) results in enhanced vasopressin V2 receptor activity and increased renal Na⁺ conservation. We hypothesized that P2Y₂R KO mice would be less sensitive to lithium-induced natriuresis and kaliuresis due to attenuated downregulation of one or more of the major renal Na⁺ or K⁺ transporter/channel proteins. KO and wild-type (WT) mice were fed a control or lithium-added diet (40 mmol/kg food) for 14 days. Lithium-induced natriuresis and kaliuresis were significantly (~25%) attenuated in KO mice. The subunits of the epithelial Na⁺ channel (ENaC) were variably affected by lithium and genotype, but, overall, medullary levels were decreased substantially by lithium (15-60%) in both genotypes. In contrast, cortical, β-, and γ-ENaC were increased by lithium (~50%), but only in WT mice. Moreover, an assessment of ENaC activity by benzamil sensitivity suggested that lithium increased ENaC activity in WT mice but in not KO mice. In contrast, medullary levels of Na⁺-K⁺-2Cl⁻ cotransporter 2 and cortical levels of the renal outer medullary K⁺ channel were not downregulated by lithium and were significantly (15-76%) higher in KO mice under both dietary conditions. In addition, under control conditions, tissue osmolality of the inner medulla as well as furosemide sensitivity were significantly higher in KO mice versus WT mice. Therefore, we suggest that increased expression of these proteins, particularly in the control state, reduces Na⁺ delivery to the distal nephron and provides a buffer to attenuate collecting duct-mediated natriuresis and kaliuresis. Additional studies are warranted to explore the potential therapeutic benefits of purinergic antagonism.
authors.affiliation: Nephrology Research, Department of Veterans Administration Salt Lake City Health Care System, Salt Lake City, UT 84148, USA.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.forename: Lijun
authors.initials: L
authors.lastname: Li
authors.forename: Donald E
authors.initials: DE
authors.lastname: Kohan
authors.forename: Carolyn M
authors.initials: CM
authors.lastname: Ecelbarger
authors.forename: Bellamkonda K
authors.initials: BK
authors.lastname: Kishore
mesh_terms: D000450:Aldosterone / Q000378:metabolism; D000704:Analysis of Variance; D000818:Animals; D004032:Diet; D004232:Diuretics / Q000494:pharmacology; D053503:Epithelial Sodium Channels / Q000378:metabolism; D005838:Genotype; D007668:Kidney / Q000378:metabolism; D007679:Kidney Medulla / Q000187:drug effects / Q000378:metabolism; D007685:Kidney Tubules, Collecting / Q000187:drug effects / Q000378:metabolism; D008094:Lithium / Q000494:pharmacology* / Q000633:toxicity; D051379:Mice; D018345:Mice, Knockout; D009318:Natriuresis / Q000187:drug effects*; D009399:Nephrons / Q000187:drug effects / Q000378:metabolism; D009994:Osmolar Concentration; D011188:Potassium / Q000652:urine*; D058473:Receptors, Purinergic P2Y2 / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D028021:Sodium-Potassium-Chloride Symporters / Q000378:metabolism; D064466:Solute Carrier Family 12, Member 1
chemical_list: D004232:Diuretics; D053503:Epithelial Sodium Channels; D058473:Receptors, Purinergic P2Y2; C578715:Slc12a1 protein, mouse; D028021:Sodium-Potassium-Chloride Symporters; D064466:Solute Carrier Family 12, Member 1; D000450:Aldosterone; D008094:Lithium; D011188:Potassium
keywords: ATP; aldosterone; epithelial sodium channel; nephrogenic diabetes insipidus; potassium channels; sodium transporters; vasopressin