title: Involvement of transmitters in the anxiolytic action of urocortin 3 in mice.
abstract: Urocortin 3 (Ucn 3) was tested for anxiolytic action in mice an elevated plus maze. For detection of the possible involvement of neurotransmitters, the mice were pretreated with receptor blockers: haloperidol, phenoxybenzamine, propranolol, atropine, methysergide, bicuculline or naloxone. The peptide was administered into the lateral brain ventricle; the receptor blockers were applied intra- peritoneally. Ucn 3 alone elicited dose-dependent bell-shaped anxiolytic action. The most effective dose was 0.5 μg. In the combined testing a 0.5 μg dose was used. Haloperidol, propranolol, atropine, methysergide, and naloxone, blocked the Ucn 3-induced anxiolytic action, while phenoxybenzamine and bicuculline were ineffective. The results suggest that dopaminergic, beta-adrenergic, cholinergic, serotonergic and opiate transmissions are involved in the anxiolytic action of Ucn 3.
authors.affiliation: Department of Pathophysiology, University of Szeged, MTA-SZTE Neurohumoral Research Group, P.O. Box 427, H-6701 Szeged, Hungary. telegdy@patph.szote.u-szeged.hu
authors.forename: G
authors.initials: G
authors.lastname: Telegdy
authors.forename: A
authors.initials: A
authors.lastname: Adamik
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001007:Anxiety / Q000139:chemically induced*; D001285:Atropine / Q000627:therapeutic use; D001640:Bicuculline / Q000627:therapeutic use; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D006220:Haloperidol / Q000627:therapeutic use; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D008784:Methysergide / Q000627:therapeutic use; D051379:Mice; D009270:Naloxone / Q000627:therapeutic use; D018377:Neurotransmitter Agents / Q000378:metabolism* / Q000627:therapeutic use; D011433:Propranolol / Q000627:therapeutic use; D013997:Time Factors; D054832:Urocortins / Q000187:drug effects / Q000633:toxicity*
chemical_list: D018377:Neurotransmitter Agents; D054832:Urocortins; D009270:Naloxone; D001285:Atropine; D011433:Propranolol; D006220:Haloperidol; D008784:Methysergide; D001640:Bicuculline
keywords: Urocortin 3 anxiolytic action