title: Fast and robust method to determine phenoyl and acetyl esters of polysaccharides by quantitative ¹H NMR.
abstract: The acetyl (AcE), feruloyl (FE), and p-coumaroyl (pCE) ester contents of different cereal and grass polysaccharides were determined by a quantitative ¹H NMR-based method. The repeatability and the robustness of the method were demonstrated by analyzing different plant polysaccharide preparations. Good sensitivity and selectivity for AcE, FE, and pCE were observed. Moreover, an optimized and easy sample preparation allowed for simultaneous quantification of AcE, FE, and pCE. The method is suitable for high-throughput analysis, and it is a good alternative for currently used analytical procedures. A comparison of the method presented to a conventional HPLC-based method showed that the results obtained are in good agreement, whereas the combination of the optimized sample preparation and analysis by the ¹H NMR-based methodology results in significantly reduced analysis time.
authors.affiliation: DSM Biotechnology Center, P.O. Box 1, 2600 MA Delft, The Netherlands.
authors.forename: Klaus G
authors.initials: KG
authors.lastname: Neumüller
authors.forename: Adriana
authors.initials: A
authors.lastname: Carvalho de Souza
authors.forename: Joep
authors.initials: J
authors.lastname: Van Rijn
authors.forename: Maaike M
authors.initials: MM
authors.lastname: Appeldoorn
authors.forename: Hugo
authors.initials: H
authors.lastname: Streekstra
authors.forename: Henk A
authors.initials: HA
authors.lastname: Schols
authors.forename: Harry
authors.initials: H
authors.lastname: Gruppen
mesh_terms: D000107:Acetylation; D000821:Animal Feed / Q000032:analysis; D002473:Cell Wall / Q000737:chemistry* / Q000378:metabolism; D018556:Crops, Agricultural / Q000737:chemistry* / Q000378:metabolism; D004952:Esters; D005504:Food Analysis / Q000379:methods*; D009426:Netherlands; D010636:Phenols / Q000032:analysis / Q000737:chemistry; D011134:Polysaccharides / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism
chemical_list: D004952:Esters; D010636:Phenols; D011134:Polysaccharides