title: Global DNA methylation status in laryngeal cancer.
abstract: BACKGROUND
  The purpose of our study was to evaluate if DNA methylation level in leukocytes may be used as a surrogate marker of genome methylation status in laryngeal cancer tissues.
  
  
  METHODS
  We evaluated global DNA methylation using an ultra performance liquid chromatography (UPLC)-based method to assess the total content of 5-methylcytosine (5 mC).
  
  
  RESULTS
  The study was performed on DNA isolated from cancer tissues, adjacent normal tissues, and peripheral blood leukocytes in a group of 72 patients with laryngeal cancer. DNA hypomethylation was found in tumor tissue (56%) and normal tissue (49%). There was a significant correlation between the levels of 5 mC in these 2 types of tissue. There was no significant DNA hypomethylation in blood. A negative correlation between tumor grade and blood levels of 5 mC was found.
  
  
  CONCLUSIONS
  The level of leukocyte DNA methylation measured using total 5 mC content cannot be used as a surrogate marker for genome methylation status in laryngeal cancer tissues.
authors.affiliation: Department of Genetics, Wroclaw Medical University, Wroclaw, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Stembalska
authors.forename: Przemysław
authors.initials: P
authors.lastname: Leszczyński
authors.forename: Justyna
authors.initials: J
authors.lastname: Gil
authors.forename: David
authors.initials: D
authors.lastname: Ramsey
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Pitala
authors.forename: Adam
authors.initials: A
authors.lastname: Maciejczyk
authors.forename: Marcin
authors.initials: M
authors.lastname: Frączek
mesh_terms: D044503:5-Methylcytosine / Q000378:metabolism; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000378:metabolism; D002853:Chromatography, Liquid; D019175:DNA Methylation; D057890:Epigenomics; D005260:Female; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007822:Laryngeal Neoplasms / Q000378:metabolism*; D008297:Male; D008875:Middle Aged
chemical_list: D015415:Biomarkers; D044503:5-Methylcytosine
keywords: 5-methylcytosine; DNA hypomethylation; epigenetics; laryngeal cancer; ultra performance liquid chromatography