title: Fast optical sectioning obtained by structured illumination microscopy using a digital mirror device.
abstract: High-throughput optical imaging is critical to obtain large-scale neural connectivity information of brain in neuroscience. Using a digital mirror device and a scientific complementary metal-oxide semiconductor camera, we report a significant speed improvement of structured illumination microscopy (SIM), which produces a maximum SIM net frame rate of 133 Hz. We perform three-dimensional (3-D) imaging of mouse brain slices at diffraction-limited resolution and demonstrate the fast 3-D imaging capability to a large sample with an imaging rate of 6.9×10(7)  pixel/s of our system, an order of magnitude faster than previously reported.
authors.affiliation: Huazhong University of Science and Technology, Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Wuhan 430074, China.
authors.forename: Dongli
authors.initials: D
authors.lastname: Xu
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.forename: Anan
authors.initials: A
authors.lastname: Li
authors.forename: Bihe
authors.initials: B
authors.lastname: Hu
authors.forename: Zhao
authors.initials: Z
authors.lastname: Feng
authors.forename: Hui
authors.initials: H
authors.lastname: Gong
authors.forename: Shaoqun
authors.initials: S
authors.lastname: Zeng
authors.forename: Qingming
authors.initials: Q
authors.lastname: Luo
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology; D003952:Diagnostic Imaging; D004867:Equipment Design; D007091:Image Processing, Computer-Assisted; D008027:Light; D008029:Lighting / Q000295:instrumentation* / Q000379:methods; D008670:Metals / Q000737:chemistry; D051379:Mice; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D018715:Microscopy, Video / Q000295:instrumentation* / Q000379:methods; D008863:Microspheres; D009474:Neurons / Q000378:metabolism; D055095:Optics and Photonics; D010087:Oxides / Q000737:chemistry; D012666:Semiconductors
chemical_list: D008670:Metals; D010087:Oxides