title: Simple tube centrifugation for processing platelet-rich plasma in the horse.
abstract: This study evaluated the quality and bacteriologic safety of platelet-rich plasma (PRP) produced by 3 simple, inexpensive tube centrifugation methods and a commercial system. Citrated equine blood collected from 26 normal horses was processed by 4 methods: blood collection tubes centrifuged at 1200 and 2000 × g, 50-mL conical tube, and a commercial system. White blood cell (WBC), red blood cell (RBC), and platelet counts and mean platelet volume (MPV) were determined for whole blood and PRP, and aerobic and anaerobic cultures were performed. Mean platelet concentrations ranged from 1.55- to 2.58-fold. The conical method yielded the most samples with platelet concentrations greater than 2.5-fold and within the clinically acceptable range of > 250,000 platelets/μL. White blood cell counts were lowest with the commercial system and unacceptably high with the blood collection tubes. The conical tube method may offer an economically feasible and comparatively safe alternative to commercial PRP production systems.
authors.affiliation: Department of Large Animal Clinical Sciences, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg, Virginia 24061-0442, USA.
authors.forename: Robin L
authors.initials: RL
authors.lastname: Fontenot
authors.forename: Carolyn A
authors.initials: CA
authors.lastname: Sink
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Werre
authors.forename: Nicole M
authors.initials: NM
authors.lastname: Weinstein
authors.forename: Linda A
authors.initials: LA
authors.lastname: Dahlgren
mesh_terms: D000818:Animals; D002498:Centrifugation / Q000379:methods / Q000662:veterinary*; D004906:Erythrocyte Count / Q000379:methods / Q000662:veterinary; D005260:Female; D006734:Horse Diseases / Q000097:blood / Q000628:therapy; D006736:Horses / Q000097:blood*; D007958:Leukocyte Count / Q000379:methods / Q000662:veterinary; D008297:Male; D010976:Platelet Count / Q000379:methods / Q000662:veterinary*; D053657:Platelet-Rich Plasma / Q000166:cytology*