title: Tyrosine phosphorylation regulates the activity of phytochrome photoreceptors.
abstract: Phytochromes are red/far-red light receptors that function in photomorphogenesis of plants. Photoisomerization of phytochrome by red light leads to its translocation to the nucleus, where it regulates gene expression. We examined whether phytochrome is phosphorylated in response to light, and we report that phytochrome B (phyB)'s N terminus contains a region with a number of phosphoserines, threonines, and tyrosines. The light-dependent phosphorylation of tyrosine 104 (Y104) appears to play a negative role in phyB's activity, because a phosphomimic mutant, phyBY104E, is unable to complement any phyB-related phenotype, is defective in binding to its signaling partner PIF3, and fails to form stable nuclear bodies even though it retains normal photochemistry in vitro. In contrast, plants stably expressing a nonphosphorylatable mutant, phyBY104F, are hypersensitive to light. The proper response to changes in the light environment is crucial for plant survival, and our study brings tyrosine phosphorylation to the forefront of light-signaling mechanisms.
authors.affiliation: Plant Biology Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: Kazumasa
authors.initials: K
authors.lastname: Nito
authors.forename: Catherine C L
authors.initials: CC
authors.lastname: Wong
authors.forename: John R
authors.initials: JR
authors.lastname: Yates
authors.forename: Joanne
authors.initials: J
authors.lastname: Chory
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000166:cytology / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D010766:Phosphorylation; D010786:Photoreceptor Cells / Q000166:cytology / Q000378:metabolism*; D010834:Phytochrome / Q000378:metabolism*; D030821:Plants, Genetically Modified; D015398:Signal Transduction; D014443:Tyrosine / Q000378:metabolism*
chemical_list: D010834:Phytochrome; D014443:Tyrosine