title: The complete mitochondrial genome of the Ailanthus silkmoth, Samia cynthia cynthia (Lepidoptera: Saturniidae).
abstract: The complete mitochondrial genome (mitogenome) of the Ailanthus silkmoth, Samia cynthia cynthia (Lepidoptera: Saturniidae) was determined. The circular genome is 15,345 bp long, and presents a typical gene organization and order for sequenced mitogenomes of Bombycidea species. The nucleotide composition of the genome is highly A+T biased, accounting for 79.86%. The AT skew of the genome is slightly negative, indicating the occurrence of more Ts than As, as found in other Saturniidae species. All protein-coding genes (PCGs) are initiated by ATN codons, except for COI and COII, which are tentatively designated by CGA and GTG, respectively, as observed in other insects. Four of 13 PCGs, including COI, COII, ATP6, and ND3, harbor the incomplete termination codons, T or TA. With an exception for tRNASer(AGN), all other tRNAs can form a typical clover-leaf structure of mitochondrial tRNA. The 359 bp A+T-rich region of S. c. cynthia contains non-repetitive sequences, but harbors several features common to the Bombycidea insects, including the motif ATAGA followed by a poly-T stretch of 19 bp, a microsatellite-like (AT)7 element preceded by the ATTTA motif, and a poly-A element upstream tRNAMet. The phylogenetic analyses support the morphology-based current hypothesis that Bombycidae and Saturniidae are monophyletic. Our result confirms that Saturniini and Attacini form a reciprocal monophyletic group within Saturniidae.
authors.affiliation: Department of Applied Biology, College of Life Science, Soochow University, Suzhou 215123, China.
authors.forename: Yang-Hu
authors.initials: YH
authors.lastname: Sima
authors.forename: Mo
authors.initials: M
authors.lastname: Chen
authors.forename: Rui
authors.initials: R
authors.lastname: Yao
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Li
authors.forename: Teng
authors.initials: T
authors.lastname: Liu
authors.forename: Xin
authors.initials: X
authors.lastname: Jin
authors.forename: Li-peng
authors.initials: LP
authors.lastname: Wang
authors.forename: Jun-Fang
authors.initials: JF
authors.lastname: Su
authors.forename: Xi-Sheng
authors.initials: XS
authors.lastname: Li
authors.forename: Yan-Qun
authors.initials: YQ
authors.lastname: Liu
mesh_terms: D000818:Animals; D001482:Base Composition; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics*; D021901:DNA, Intergenic / Q000737:chemistry; D023061:Gene Order; D054629:Genome, Mitochondrial; D023281:Genomics; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames; D010802:Phylogeny; D012335:RNA, Ribosomal / Q000235:genetics; D012343:RNA, Transfer / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment
chemical_list: D021901:DNA, Intergenic; D012335:RNA, Ribosomal; D012343:RNA, Transfer
keywords: ATP; COX; F(0) ATPase; Mitochondrial genome; PCGs; Phylogenetic analysis; Samia cynthia cynthia; Saturniidae; cytochrome oxidase subunits, CytB, cytochrome B, ND, NADH dehydrogenase; large subunit ribosomal RNA; lrRNA; mitochondrial DNA; mitochondrial genome; mitogenome; mtDNA; protein-coding genes; seRNA; small subunit ribosomal RNA