title: Protein sample characterization.
abstract: Most biophysical experiments require protein samples of high quality and accurately determined concentration. This chapter attempts to compile basic information on the most common methods to assess the purity, dispersity, and stability of protein samples. It also reminds of methods to measure protein concentration and of their limits. The idea is to make aware and remind of the range of methods available and of commonly overlooked pitfalls. The aim is to enable experimenters to fully characterize their preparations of soluble or membrane proteins and gain reliable and reproducible results from their experimental work.
authors.affiliation: ISMB Biophysics Centre, Institute of Structural and Molecular Biology, Birkbeck, University of London, London, UK.
authors.forename: Tina
authors.initials: T
authors.lastname: Daviter
authors.forename: Rémi
authors.initials: R
authors.lastname: Fronzes
mesh_terms: D002850:Chromatography, Gel; D002942:Circular Dichroism; D004591:Electrophoresis, Polyacrylamide Gel; D008027:Light; D008565:Membrane Proteins / Q000737:chemistry* / Q000302:isolation & purification; D055550:Protein Stability; D012542:Scattering, Radiation; D012995:Solubility; D014461:Ultracentrifugation
chemical_list: D008565:Membrane Proteins