title: Characterization of Dak Nong virus, an insect nidovirus isolated from Culex mosquitoes in Vietnam.
abstract: In this study, we isolated and characterized an insect nidovirus from the mosquito Culex tritaeniorhynchus Giles (Diptera: Culicidae) in Vietnam, as an additional member of the new family Mesoniviridae in the order Nidovirales. The virus, designated "Dak Nong virus (DKNV)," shared many characteristics with Cavally virus and Nam Dinh virus, which have also been discovered recently in mosquitoes, and these viruses should be considered members of a single virus species, Alphamesonivirus 1. DKNV grew in cultured mosquito cells but could not replicate in the cultured vertebrate cells tested. N-terminal sequencing of the DKNV structural proteins revealed two posttranslational cleavage sites in the spike glycoprotein precursor. DKNV is assumed to be a new member of the species Alphamesonivirus 1, and the current study provides further understanding of viruses belonging to the new family Mesoniviridae.
authors.affiliation: Department of Medical Entomology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan.
authors.forename: Ryusei
authors.initials: R
authors.lastname: Kuwata
authors.forename: Tomomitsu
authors.initials: T
authors.lastname: Satho
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Isawa
authors.forename: Nguyen Thi
authors.initials: NT
authors.lastname: Yen
authors.forename: Tran Vu
authors.initials: TV
authors.lastname: Phong
authors.forename: Phan Thi
authors.initials: PT
authors.lastname: Nga
authors.forename: Tomokazu
authors.initials: T
authors.lastname: Kurashige
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Hiramatsu
authors.forename: Yuki
authors.initials: Y
authors.lastname: Fukumitsu
authors.forename: Keita
authors.initials: K
authors.lastname: Hoshino
authors.forename: Toshinori
authors.initials: T
authors.lastname: Sasaki
authors.forename: Mutsuo
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Mizutani
authors.forename: Kyoko
authors.initials: K
authors.lastname: Sawabe
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002522:Chlorocebus aethiops; D006224:Cricetinae; D003465:Culex / Q000821:virology*; D005260:Female; D007304:Insect Viruses / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D028381:Nidovirales / Q000145:classification* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D014709:Vero Cells; D014744:Vietnam; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D014764:Viral Proteins