title: [Detection of periodontal pathogens from saliva of type 2 diabetic patients in urban area of Beijing].
abstract: OBJECTIVE
  To investigate the prevalence of periodontal pathogens from saliva of patients with type 2 diabetes mellitus (T2DM), and to characterize the association between the glucose status and periodontal pathogens in oral cavity.
  
  
  METHODS
  All the subjects were hypertension patients under regular care at Beijing hypertension prevention and management institute. Whole unstimulated saliva samples were collected from 45 non-diabetic subjects (non-DM group), 80 well-controlled diabetic patients (DM-well group) and 100 poor-controlled diabetic patients (DM-poor group). DNA was extracted from the salivary deposition, Porphyromonas gingivalis (Pg), Tannerella forsythia (Tf) and Treponema denticola (Td) were detected by polymerase chain reaction (PCR) method based on 16SrRNA. Prevalence and quantity of the pathogens under different glucose states were compared and logistic regression model was set to analyze the factors related to each bacterium.
  
  
  RESULTS
  The prevalence of Tf in DM-well group and DM-poor group was significantly lower than that of non-DM group [81% (65/80), 80% (80/100) vs 91% (41/45), P = 0.048], meanwhile the quantity of Tf was also lower than that of non-DM group [1.9(2.6), 2.1(5.3) vs 3.4(6.4)] (P > 0.05). With the worsening of glucose control, the quantity of Tf was declining (P = 0.032). However, the prevalence and the quantity of Pg, Td in 3 groups had no statistical differences (P > 0.05). After adjusting age, gender, number of missing teeth and other periodontal parameters, OR of having Tf in saliva from DM-well group and DM-poor group was 0.58 and 0.53, respectively.
  
  
  CONCLUSIONS
  Abnormal blood glucose state may affect the colonization of Tf in oral cavity.
authors.affiliation: Department of Periodotology, Peking University School and Hospital of Stomatology, Beijing, China.
authors.forename: Yan-ting
authors.initials: YT
authors.lastname: Liao
authors.forename: Lu
authors.initials: L
authors.lastname: He
authors.forename: Huan-xin
authors.initials: HX
authors.lastname: Meng
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.forename: Yue-qin
authors.initials: YQ
authors.lastname: Sha
authors.forename: Xing-yu
authors.initials: XY
authors.lastname: Wang
mesh_terms: D000368:Aged; D002681:China / Q000453:epidemiology; D003924:Diabetes Mellitus, Type 2 / Q000453:epidemiology / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010518:Periodontitis / Q000382:microbiology*; D016966:Porphyromonas gingivalis / Q000302:isolation & purification; D012463:Saliva / Q000382:microbiology*; D048373:Treponema denticola / Q000302:isolation & purification