title: Hypermethylation and low transcription of TLR2 gene in chronic periodontitis.
abstract: Periodontitis is an inflammatory disorder characterized by interactions between periodontal pathogens and host's immune response. Epigenetic may contribute to disease development and outcome by influencing the expression of genes involved in the immune response. It has been shown that Toll-like receptors (TLR) play an important role in the response to periodontopathic bacteria. The aim of study was to evaluate the methylation status and the expression of TLR2 gene in gingival samples from individuals with and without periodontitis. DNA was analyzed using the Methyl Profiler DNA Methylation qPCR assay. DNA methylation and transcript levels were evaluated by real-time polymerase chain reaction. The periodontitis group showed a hypermethylated profile and a low expression of gene. Positive correlation between the TLR2 methylation frequency and probing depth was observed. This study gives the first evidence of methylation frequency in inflamed periodontal tissues and of the possible participation of methylation in the development of periodontitis.
authors.affiliation: Departamento de Clínica, Patologia e Cirurgia Odontológicas, Faculdade de Odontologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
authors.forename: Simone Angélica
authors.initials: SA
authors.lastname: de Faria Amormino
authors.forename: Telma Cristina
authors.initials: TC
authors.lastname: Arão
authors.forename: Adriana Machado
authors.initials: AM
authors.lastname: Saraiva
authors.forename: Ricardo Santiago
authors.initials: RS
authors.lastname: Gomez
authors.forename: Walderez Ornelas
authors.initials: WO
authors.lastname: Dutra
authors.forename: José Eustáquio
authors.initials: JE
authors.lastname: da Costa
authors.forename: Jeane
authors.initials: J
authors.lastname: de Fátima Correia Silva
authors.forename: Paula Rocha
authors.initials: PR
authors.lastname: Moreira
mesh_terms: D000328:Adult; D055113:Chronic Periodontitis / Q000235:genetics*; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D005260:Female; D005881:Gingiva / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D051195:Toll-Like Receptor 2 / Q000235:genetics*; D014158:Transcription, Genetic
chemical_list: C495342:TLR2 protein, human; D051195:Toll-Like Receptor 2