title: Altered hepatic gene expression profiles associated with improved fatty liver, insulin resistance, and intestinal permeability after hydroxypropyl methylcellulose (HPMC) supplementation in diet-induced obese mice.
abstract: The effect of hydroxypropyl methylcellulose (HPMC) on hepatic gene expression was analyzed by exon microarray and real-time PCR from livers of diet-induced obese (DIO) mice fed a high-fat (HF) diet supplemented with either 6% HPMC or 6% microcrystalline cellulose (MCC). HPMC-fed mice exhibited significantly reduced body weight gain (55% lower compared to MCC), liver weight (13%), plasma LDL-cholesterol concentration (45%), and HF diet-increased intestinal permeability (48%). HPMC significantly reduced areas under the curve for 2 h insulin and glucose responses, indicating enhanced insulin sensitivity and glucose metabolism. HPMC up-regulated hepatic genes related to fatty acid oxidation, cholesterol and bile acid synthesis, and cellular activation of glucocorticoid (bile acid recycling) and down-regulated genes related to oxidative stress, triglyceride synthesis, and polyunsaturated fatty acid elongation. In conclusion, HPMC consumption ameliorates the effects of a HF diet on intestinal permeability, insulin resistance, hepatic lipid accumulation, glucocorticoid-related bile acid recycling, oxidative stress, and weight gain in DIO mice.
authors.affiliation: Department of Nutrition, University of California, Davis, California 95616, USA. hyskim@ucdavis.edu
authors.forename: Hyunsook
authors.initials: H
authors.lastname: Kim
authors.forename: Glenn E
authors.initials: GE
authors.lastname: Bartley
authors.forename: Scott A
authors.initials: SA
authors.lastname: Young
authors.forename: Kun-Ho
authors.initials: KH
authors.lastname: Seo
authors.forename: Wallace
authors.initials: W
authors.lastname: Yokoyama
mesh_terms: D000818:Animals; D002482:Cellulose / Q000627:therapeutic use; D059305:Diet, High-Fat / Q000009:adverse effects; D005234:Fatty Liver / Q000209:etiology / Q000517:prevention & control*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D065347:Hypromellose Derivatives; D007333:Insulin Resistance; D007408:Intestinal Absorption; D008099:Liver / Q000201:enzymology / Q000378:metabolism*; D008747:Methylcellulose / Q000031:analogs & derivatives / Q000627:therapeutic use; D051379:Mice; D065626:Non-alcoholic Fatty Liver Disease; D009765:Obesity / Q000178:diet therapy* / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology; D056692:Prebiotics
chemical_list: D056692:Prebiotics; D065347:Hypromellose Derivatives; D002482:Cellulose; D008747:Methylcellulose; C109691:microcrystalline cellulose