title: Inhibitory activity of benzophenones from Anemarrhena asphodeloides on pancreatic lipase.
abstract: Pancreatic lipase is a key enzyme for lipid absorption by hydrolysis of total dietary fats. Therefore, inhibition of pancreatic lipase is suggested to be an effective therapy in the regulation of obesity. The EtOAc-soluble fraction of Anemarrhena asphodeloides rhizomes significantly inhibited pancreatic lipase activity as assessed using porcine pancreatic lipase as an in vitro assay system. Further fractionation of the EtOAc-soluble fraction of A. asphodeloides led to the isolation of a new benzophenone glycoside, zimoside A (1), together with the eleven known compounds iriflophenone (2), 2,4',6-trihydroxy-4-methoxybenzophenone (3), foliamangiferoside A (4), (2,3-dihydroxy-4-methoxyphenyl)(4-hydroxyphenyl)-methanone (5), 1,4,5,6,-tetrahydroxyxanthone (6), isosakuranetin (7), 4-hydroxybenzoic acid (8), 4-hydroxyacetophenone (9), vanillic acid (10), tyrosol (11) and 5-hydroxymethyl-2-furaldehyde (12). Among the isolated compounds, 3, 5 and 10 showed significant inhibition of pancreatic lipase activity.
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju 361-763, Korea.
authors.forename: Yang Hee
authors.initials: YH
authors.lastname: Jo
authors.forename: Seon Beom
authors.initials: SB
authors.lastname: Kim
authors.forename: Jong Hoon
authors.initials: JH
authors.lastname: Ahn
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.forename: Bang Yeon
authors.initials: BY
authors.lastname: Hwang
authors.forename: Mi Kyeong
authors.initials: MK
authors.lastname: Lee
mesh_terms: D031403:Anemarrhena / Q000737:chemistry*; D001577:Benzophenones / Q000494:pharmacology*; D008049:Lipase / Q000037:antagonists & inhibitors*; D013329:Structure-Activity Relationship
chemical_list: D001577:Benzophenones; D008049:Lipase; C518354:PNLIP protein, human