title: Gene expression signature-based prognostic risk score in patients with glioblastoma.
abstract: The present study aimed to identify genes associated with patient survival to improve our understanding of the underlying biology of gliomas. We investigated whether the expression of genes selected using random survival forests models could be used to define glioma subgroups more objectively than standard pathology. The RNA from 32 non-treated grade 4 gliomas were analyzed using the GeneChip Human Genome U133 Plus 2.0 Expression array (which contains approximately 47 000 genes). Twenty-five genes whose expressions were strongly and consistently related to patient survival were identified. The prognosis prediction score of these genes was most significant among several variables and survival analyses. The prognosis prediction score of three genes and age classifiers also revealed a strong prognostic value among grade 4 gliomas. These results were validated in an independent samples set (n = 488). Our method was effective for objectively classifying grade 4 gliomas and was a more accurate prognosis predictor than histological grading.
authors.affiliation: Biostatistic Center, Kurume University School of Medicine, Kurume, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kawaguchi
authors.forename: Naoki
authors.initials: N
authors.lastname: Yajima
authors.forename: Naoto
authors.initials: N
authors.lastname: Tsuchiya
authors.forename: Jumpei
authors.initials: J
authors.lastname: Homma
authors.forename: Masakazu
authors.initials: M
authors.lastname: Sano
authors.forename: Manabu
authors.initials: M
authors.lastname: Natsumeda
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Takahashi
authors.forename: Yukihiko
authors.initials: Y
authors.lastname: Fujii
authors.forename: Tatsuyuki
authors.initials: T
authors.lastname: Kakuma
authors.forename: Ryuya
authors.initials: R
authors.lastname: Yamanaka
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D005260:Female; D005909:Glioblastoma / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D011379:Prognosis; D012313:RNA / Q000235:genetics; D016019:Survival Analysis; D059467:Transcriptome; D055815:Young Adult
chemical_list: D012313:RNA