title: Streptomyces siamensis sp. nov., and Streptomyces similanensis sp. nov., isolated from Thai soils.
abstract: Three actinomycete strains, KC-038(T), KC-031 and KC-106(T), were isolated from soil samples collected in the southern Thailand. The morphological and chemotaxonomic properties of strains KC-038(T), KC-031 and KC-106(T) were consistent with the characteristics of members of the genus Streptomyces, that is, the formation of aerial mycelia bearing spiral spore chains; the presence of LL-diaminopimelic acid in the cell wall, MK-9 (H6), MK-9 (H4) and MK-9 (H8) as the predominant menaquinones; and C16:0, iso-C16:0 and anteiso-C15:0 as the major cellular fatty acids. 16S rRNA gene sequence analyses indicated that strains KC-038(T) and KC-031 were highly similar (99.9%), and they were closely related to S. olivochromogenes NBRC 3178(T) (98.1%) and S. psammoticus NBRC 13971(T) (98.1%). Strain KC-106(T) was closely related to S. seoulensis NBRC 16668(T) (98.9%), S. recifensis NBRC 12813(T) (98.9%), S. chartreusis NBRC 12753(T) (98.7%) and S. griseoluteus NBRC 13375(T) (98.4%). The values of DNA-DNA relatedness between the isolates and the type strains of the related species were below 70%. On the basis of the polyphasic evidence, the isolates should be classified as two novel species, namely Streptomyces siamensis sp. nov. (type strain, KC-038(T) = NBRC 108799(T) = PCU 328(T) = TISTR 2107(T)) and Streptomyces similanensis sp. nov. (type strain, KC-106(T) = NBRC 108798(T) = PCU 329(T) = TISTR 2104(T)).
authors.affiliation: Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Paranee
authors.initials: P
authors.lastname: Sripreechasak
authors.forename: Atsuko
authors.initials: A
authors.lastname: Matsumoto
authors.forename: Khanit
authors.initials: K
authors.lastname: Suwanborirux
authors.forename: Yuki
authors.initials: Y
authors.lastname: Inahashi
authors.forename: Kazuro
authors.initials: K
authors.lastname: Shiomi
authors.forename: Somboon
authors.initials: S
authors.lastname: Tanasupawat
authors.forename: Yoko
authors.initials: Y
authors.lastname: Takahashi
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics*; D012329:RNA, Bacterial / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017423:Sequence Analysis, RNA / Q000379:methods; D012988:Soil Microbiology; D013302:Streptomyces / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification*; D013785:Thailand
chemical_list: D004269:DNA, Bacterial; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S