title: Production of hydroxlated flavonoids with cytochrome P450 BM3 variant F87V and their antioxidative activities.
abstract: A variant of P450 BM3 with an F87V substitution [P450 BM3 (F87V)] is a substrate-promiscuous cytochrome P450 monooxygenase. We investigated the bioconversion of various flavonoids (favanones, chalcone, and isoflavone) by using recombinant Escherichia coli cells, which expressed the gene coding for P450 BM3 (F87V), to give their corresponding hydroxylated products. Potent antioxidative activities were observed in some of the products.
authors.affiliation: Department of Food and Nutrition, Japan Women's University, Tokyo, Japan.
authors.forename: Emi
authors.initials: E
authors.lastname: Kitamura
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Otomatsu
authors.forename: Chiemi
authors.initials: C
authors.lastname: Maeda
authors.forename: Yoko
authors.initials: Y
authors.lastname: Aoki
authors.forename: Chihiro
authors.initials: C
authors.lastname: Ota
authors.forename: Norihiko
authors.initials: N
authors.lastname: Misawa
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Shindo
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics*; D005419:Flavonoids / Q000737:chemistry / Q000378:metabolism; D006899:Mixed Function Oxygenases / Q000378:metabolism; D009249:NADP / Q000737:chemistry / Q000378:metabolism; D009251:NADPH-Ferrihemoprotein Reductase / Q000235:genetics* / Q000378:metabolism; D010084:Oxidation-Reduction; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000378:metabolism; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
chemical_list: D000975:Antioxidants; D001426:Bacterial Proteins; D005419:Flavonoids; D011993:Recombinant Fusion Proteins; D009249:NADP; D003577:Cytochrome P-450 Enzyme System; D006899:Mixed Function Oxygenases; D009251:NADPH-Ferrihemoprotein Reductase; C052423:flavocytochrome P450 BM3 monoxygenases