title: Preliminary characterization of proteoglycan from Hyriopsis cumingii.
abstract: OBJECTIVE
  To study the preliminary characterizations of Hyriopsis cumingii proteoglycan (HCPG).
  
  
  METHODS
  The content of carbohydrate and protein were measured by spectrophotometry. FTIR spectrum was used to analyze the functional groups. Relative molecular mass and amino acid composition were detected by HPLC. GC was utilized to determine the monosaccharide composition. The glycopeptide linkage-bond was detected by using the method of beta-elimination reaction.
  
  
  RESULTS
  In HCPG,the content of carbohydrate and protein was 80.06% and 9.42%, respectively. FTIR spectrum showed the characteristic absorptions of polysaccharides and protein. Relative molecular mass of HCPG, determined by size-exclusive HPLC, was 503.1 kDa. GC spectra demonstrated that polysaccharide of HCPG was composed of rhamnose, fucose, mannose, glucose and galactose with a molar ratio of 13.80: 4.51: 7.70: 64.92 : 9.07. Fourteen amino acids (13 known and one unknown) have been detected by pre-column derivation HPLC. From beta-elimination reaction, peptide chain was attached to the carbohydrate chain by O-glycosidic bond.
  
  
  CONCLUSIONS
  Basic characterizations of HCPG have been determined preliminarily.
authors.affiliation: College of Biological and Pharmaceutical Engineering, West Anhui University, Liu'an 237012, China. qiaodll@wxc.edu.cn
authors.forename: De-Liang
authors.initials: DL
authors.lastname: Qiao
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.forename: Yi
authors.initials: Y
authors.lastname: Sun
authors.forename: Hong
authors.initials: H
authors.lastname: Ye
authors.forename: Xiao-Xiong
authors.initials: XX
authors.lastname: Zeng
mesh_terms: D000596:Amino Acids / Q000032:analysis; D000818:Animals; D002241:Carbohydrates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006020:Glycopeptides / Q000737:chemistry; D008970:Molecular Weight; D011506:Proteins / Q000032:analysis*; D011509:Proteoglycans / Q000737:chemistry* / Q000302:isolation & purification*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D049876:Unionidae / Q000737:chemistry*
chemical_list: D000596:Amino Acids; D002241:Carbohydrates; D006020:Glycopeptides; D011506:Proteins; D011509:Proteoglycans