title: Expression and distribution of dendritic cells in nasal polyps.
abstract: The aim of the present study was to investigate the expression, distribution and function of dendritic cells (DCs) and to study their role in nasal polyps. The study involved 55 participants, 45 of whom had nasal polyps and were the study group and 10 who had normal inferior turbinates and were the control group. Immunohistochemical staining was used to visualize the expression and distribution of the S-100 protein. A double immunostaining method was used to visualize the CD1a and CD40 expression and the images were analyzed with Axioplan 2 microscopy. The expression level of the S-100 protein in the nasal polyps was higher than that in the normal inferior turbinates with a significant difference (P<0.01). The distribution area, number and density of the double stained cells in the nasal polyps were all greater than in the normal inferior turbinates (P<0.01). The S-100 protein and double stained cells were mainly located in the lamina propria below the mucous membrane. The present study demonstrates that DCs are involved in the pathogenesis of nasal polyps and the presence of CD40-positive DCs suggests that this was related to the reciprocal interaction between the DCs and T lymphocytes.
authors.affiliation: Department of Otolarynology-Head and Neck Surgery, The Shantou Central Hospital/Affiliated Shantou Hospital of Sun Yet-Sen University, Shantou, Guangdong 515041;
authors.forename: Xin-Sheng
authors.initials: XS
authors.lastname: Lin
authors.forename: Xian-Yang
authors.initials: XY
authors.lastname: Luo
authors.forename: Hui-Ge
authors.initials: HG
authors.lastname: Wang
authors.forename: Chuang-Wei
authors.initials: CW
authors.lastname: Li
authors.forename: Xin
authors.initials: X
authors.lastname: Lin
authors.forename: Chu
authors.initials: C
authors.lastname: Yan
keywords: CD1a; CD40; S-100 protein; dendritic cells; nasal polyps; pathogenesis