title: Controlled surface modification of tissue culture polystyrene for selective cell binding using resilin-inspired polypeptides.
abstract: Modified tissue culture polystyrene (TCP) surfaces have been fabricated by attachment of recombinant polypeptides based on Drosophila melanogaster resilin and the Anopheles gambiae resilin-like protein. The D. melanogaster polypeptide (Rec-1) was from the first exon of resilin and consisted of 17 very similar repeats of a 15 residue sequence. The A. gambiae polypeptide consisted of 16 repeats of an 11 residue consensus sequence (An16). Polypeptides were attached to the TCP surface through tyrosine-based photo-crosslinking using blue light in combination with (RuII(bpy)3)Cl2 and sodium persulfate. TCP that has been manufactured by mild oxidation has surface phenolic groups that are believed to participate in this crosslinking process. X-ray photoelectron spectroscopy and contact angle analyses were used to demonstrate polypeptide binding. At higher coating concentrations of Rec-1 and An16, the surface was passivated and fibroblasts no longer attached and spread. At coating concentrations of 1 mg ml(-1) for Rec-1 and 0.1 mg ml(-1) for An16, where the surface was fully passivated against fibroblast attachment, addition of a cell attachment peptide, cyclo(Arg-Gly-Asp-D-Tyr-Lys) during coating and photo-crosslinking at >0.1 mg ml(-1), led to the restoration of fibroblast binding that was dependent on the integrin αV chain.
authors.affiliation: CSIRO Materials Science and Engineering, Private Bag 10, Clayton South, VIC 3169, Australia.
authors.forename: Aditya V
authors.initials: AV
authors.lastname: Vashi
authors.forename: John A M
authors.initials: JA
authors.lastname: Ramshaw
authors.forename: Veronica
authors.initials: V
authors.lastname: Glattauer
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Elvin
authors.forename: Russell E
authors.initials: RE
authors.lastname: Lyons
authors.forename: Jerome A
authors.initials: JA
authors.lastname: Werkmeister
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D002460:Cell Line; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology* / Q000378:metabolism; D019476:Insect Proteins / Q000378:metabolism*; D051379:Mice; D010455:Peptides / Q000378:metabolism*; D011137:Polystyrenes / Q000737:chemistry*; D013499:Surface Properties; D046509:Tissue Culture Techniques / Q000295:instrumentation*
chemical_list: D001672:Biocompatible Materials; D019476:Insect Proteins; D010455:Peptides; D011137:Polystyrenes; C030951:resilin