title: Metabonomic profiling of serum and urine by (1)H NMR-based spectroscopy discriminates patients with chronic obstructive pulmonary disease and healthy individuals.
abstract: Chronic obstructive pulmonary disease (COPD) has seriously impacted the health of individuals and populations. In this study, proton nuclear magnetic resonance ((1)H NMR)-based metabonomics combined with multivariate pattern recognition analysis was applied to investigate the metabolic signatures of patients with COPD. Serum and urine samples were collected from COPD patients (n = 32) and healthy controls (n = 21), respectively. Samples were analyzed by high resolution (1)H NMR (600 MHz), and the obtained spectral profiles were then subjected to multivariate data analysis. Consistent metabolic differences have been found in serum as well as in urine samples from COPD patients and healthy controls. Compared to healthy controls, COPD patients displayed decreased lipoprotein and amino acids, including branched-chain amino acids (BCAAs), and increased glycerolphosphocholine in serum. Moreover, metabolic differences in urine were more significant than in serum. Decreased urinary 1-methylnicotinamide, creatinine and lactate have been discovered in COPD patients in comparison with healthy controls. Conversely, acetate, ketone bodies, carnosine, m-hydroxyphenylacetate, phenylacetyglycine, pyruvate and α-ketoglutarate exhibited enhanced expression levels in COPD patients relative to healthy subjects. Our results illustrate the potential application of NMR-based metabonomics in early diagnosis and understanding the mechanisms of COPD.
authors.affiliation: Department of Respiratory Medicine, The Fourth Affiliated Hospital of China Medical University, Shenyang, People's Republic of China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Wang
authors.forename: Yufu
authors.initials: Y
authors.lastname: Tang
authors.forename: Shuo
authors.initials: S
authors.lastname: Liu
authors.forename: Shitao
authors.initials: S
authors.lastname: Mao
authors.forename: Yuan
authors.initials: Y
authors.lastname: Ling
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: He
authors.forename: Xiaoge
authors.initials: X
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000596:Amino Acids / Q000097:blood / Q000652:urine; D000597:Amino Acids, Branched-Chain / Q000097:blood / Q000652:urine; D005260:Female; D006801:Humans; D008074:Lipoproteins / Q000097:blood / Q000652:urine; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D055432:Metabolomics / Q000379:methods*; D008875:Middle Aged; D010767:Phosphorylcholine / Q000097:blood / Q000652:urine; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood* / Q000652:urine*
chemical_list: D000596:Amino Acids; D000597:Amino Acids, Branched-Chain; D008074:Lipoproteins; D010767:Phosphorylcholine