title: Screening of Lactobacillus strains for their ability to bind benzo(a)pyrene and the mechanism of the process.
abstract: In order to investigate the binding ability of Lactobacillus strains to Benzo(a)pyrene (BaP), 15 strains were analysed. L. plantarum CICC 22135 and L. pentosus CICC 23163 exhibited high efficiency in removing BaP from aqueous medium; the binding rates were 66.76% and 64.31%, respectively. This process was affected by temperature, incubation time and pH, and cell viability was not necessary for the binding ability. Additionally, both strains, especially strain CICC 23163 showed high specificity in binding BaP. The cell-BaP complexes were stable in aqueous medium. The mechanism of binding was investigated by examining the binding ability of different components of the microorganism cells. The results revealed that peptidoglycans played an important role in binding BaP and its structural integrity was required. Consequently, we proposed that the mechanism of this process was a physisorption and peptidoglycan was the main binding site. These two strains may be used for dietary detoxification in human diet and animal feed.
authors.affiliation: College of Biological Science & Biotechnology, Beijing Forestry University, 100083 Beijing, China.
authors.forename: Hongfei
authors.initials: H
authors.lastname: Zhao
authors.forename: Fang
authors.initials: F
authors.lastname: Zhou
authors.forename: Yeqiong
authors.initials: Y
authors.lastname: Qi
authors.forename: Piotr
authors.initials: P
authors.lastname: Dziugan
authors.forename: Fengling
authors.initials: F
authors.lastname: Bai
authors.forename: Piotr
authors.initials: P
authors.lastname: Walczak
authors.forename: Bolin
authors.initials: B
authors.lastname: Zhang
mesh_terms: D000327:Adsorption / Q000528:radiation effects; D001426:Bacterial Proteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism* / Q000528:radiation effects; D001564:Benzo(a)pyrene / Q000378:metabolism* / Q000633:toxicity; D001665:Binding Sites / Q000528:radiation effects; D002273:Carcinogens / Q000378:metabolism* / Q000633:toxicity; D002473:Cell Wall / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D005506:Food Contamination / Q000517:prevention & control*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007778:Lactobacillus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism* / Q000528:radiation effects; D048191:Lactiplantibacillus plantarum / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects; D008562:Membrane Glycoproteins / Q000032:analysis / Q000737:chemistry / Q000378:metabolism; D050296:Microbial Viability; D010457:Peptidoglycan / Q000032:analysis / Q000737:chemistry / Q000378:metabolism* / Q000528:radiation effects; D055550:Protein Stability / Q000528:radiation effects; D013010:Sonication; D013104:Spheroplasts / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D013499:Surface Properties / Q000528:radiation effects; D013682:Teichoic Acids / Q000032:analysis / Q000378:metabolism; D013696:Temperature
chemical_list: D001426:Bacterial Proteins; D002273:Carcinogens; D008562:Membrane Glycoproteins; D010457:Peptidoglycan; C055764:S-layer proteins; D013682:Teichoic Acids; D001564:Benzo(a)pyrene
keywords: Benzo(a)pyrene; Lactobacillus; binding; mechanism