title: Diversity of gene cassette promoter variants of class 1 integrons in uropathogenic Escherichia coli.
abstract: Class 1 integrons play important roles in the emergence and horizontal transfer of antibiotic resistance genes among bacteria. The gene cassette promoter variants Pc or Pc-P2 of class 1 integrons not only drive the transcription of downstream gene cassettes, they also correlate with the excision and integration efficiency of the capture exogenous gene cassettes. In this study, the diversity of Pc or Pc-P2 variants of class 1 integrons and their association with antibiotic resistance phenotypes were analyzed in 132 uropathogenic Escherichia coli strains. Class 1 integrons were detected in 95 (72 %) strains. Sixteen different gene cassettes, 11 different gene cassette arrays and six different Pc or Pc-P2 variants were detected. The most prevalent gene cassettes were those that conferred resistance to trimethoprim, aminoglycosides, and chloramphenicol. The most prevalent promoter was PcH1, a relatively weak promoter. Certain gene cassette arrays or gene cassettes were mainly associated with the same Pc or Pc-P2 in different strains. Strains harboring class 1 integrons with relatively strong promoters had higher resistance rates to, or higher MIC(50) for, amikacin, chloramphenicol and tobramycin than those with relatively weak promoters. To the best of our knowledge, this is the first report of the diversity of class 1 integron Pc or Pc-P2 variants in uropathogenic E. coli strains.
authors.affiliation: Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, 12 Central Urumqi Road, Shanghai, 200040, China.
authors.forename: Quhao
authors.initials: Q
authors.lastname: Wei
authors.forename: Xiaofei
authors.initials: X
authors.lastname: Jiang
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.forename: Qingfeng
authors.initials: Q
authors.lastname: Hu
authors.forename: Huoxiang
authors.initials: H
authors.lastname: Lu
authors.forename: Guoqiang
authors.initials: G
authors.lastname: Chen
authors.forename: Yonglie
authors.initials: Y
authors.lastname: Zhou
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lu
mesh_terms: D024881:Drug Resistance, Bacterial / Q000235:genetics; D014644:Genetic Variation; D006801:Humans; D032023:Integrons; D010641:Phenotype; D011401:Promoter Regions, Genetic; D056689:Uropathogenic Escherichia coli / Q000187:drug effects / Q000235:genetics*