title: Lobe-related concentration- and Ca(2+)-dependent interactions of calmodulin with C- and N-terminal tails of the CaV1.2 channel.
abstract: This study examined the bindings of calmodulin (CaM) and its mutants with the C- and N-terminal tails of the voltage-gated Ca(2+) channel CaV1.2 at different CaM and Ca(2+) concentrations ([Ca(2+)]) by using the pull-down assay method to obtain basic information on the binding mode, including its concentration- and Ca(2+)-dependencies. Our data show that more than one CaM molecule could bind to the CaV1.2 C-terminal tail at high [Ca(2+)]. Additionally, the C-lobe of CaM is highly critical in sensing the change of [Ca(2+)] in its binding to the C-terminal tail of CaV1.2, and the binding between CaM and the N-terminal tail of CaV1.2 requires high [Ca(2+)]. Our data provide new details on the interactions between CaM and the CaV1.2 channel.
authors.affiliation: Department of Pharmaceutical Toxicology, School of Pharmaceutical Science, China Medical University, Shenyang 110001, China.
authors.forename: Guilin
authors.initials: G
authors.lastname: He
authors.forename: Feng
authors.initials: F
authors.lastname: Guo
authors.forename: Tong
authors.initials: T
authors.lastname: Zhu
authors.forename: Dongxue
authors.initials: D
authors.lastname: Shao
authors.forename: Rui
authors.initials: R
authors.lastname: Feng
authors.forename: Dandan
authors.initials: D
authors.lastname: Yin
authors.forename: Xuefei
authors.initials: X
authors.lastname: Sun
authors.forename: Huiyuan
authors.initials: H
authors.lastname: Hu
authors.forename: Ahhyeon
authors.initials: A
authors.lastname: Hwang
authors.forename: Etsuko
authors.initials: E
authors.lastname: Minobe
authors.forename: Masaki
authors.initials: M
authors.lastname: Kameyama
authors.forename: Liying
authors.initials: L
authors.lastname: Hao
mesh_terms: D000595:Amino Acid Sequence; D002118:Calcium / Q000378:metabolism*; D020746:Calcium Channels, L-Type / Q000378:metabolism*; D002147:Calmodulin / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D009154:Mutation; D011485:Protein Binding
chemical_list: D020746:Calcium Channels, L-Type; D002147:Calmodulin; C419385:L-type calcium channel alpha(1C); D002118:Calcium