title: NS1' colocalizes with NS1 and can substitute for NS1 in West Nile virus replication.
abstract: NS1' is a C-terminally extended form of the NS1 protein produced only by encephalitic flaviviruses from the Japanese encephalitis virus serogroup. Here we show that West Nile virus (WNV) NS1' and NS1 localize to the same cellular compartments when expressed from plasmid DNAs and also colocalize to viral RNA replication sites in infected cells. Using complementation analysis with NS1-deleted WNV cDNA, we demonstrated that NS1' is able to substitute for the crucial function of NS1 in virus replication.
authors.affiliation: Australian Infectious Disease Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland, Brisbane, Australia.
authors.forename: Lucy B
authors.initials: LB
authors.lastname: Young
authors.forename: Ezequiel Balmori
authors.initials: EB
authors.lastname: Melian
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Khromykh
mesh_terms: D002460:Cell Line; D003593:Cytoplasm / Q000737:chemistry; D005816:Genetic Complementation Test; D006801:Humans; D021381:Protein Transport; D012367:RNA, Viral / Q000378:metabolism; D017361:Viral Nonstructural Proteins / Q000235:genetics / Q000378:metabolism*; D014779:Virus Replication; D014902:West Nile virus / Q000235:genetics / Q000502:physiology*
chemical_list: D012367:RNA, Viral; D017361:Viral Nonstructural Proteins; C531936:nonstructural protein 1, West Nile virus