title: Therapeutic option for managing lung injury induced by infrarenal aortic cross-clamping.
abstract: BACKGROUND
  Operations on the infrarenal aorta can cause ischemic-reperfusion (IR) injury in local tissues, which could result in remote organ (e.g., lung) damage. Treatment of such injuries remains an unresolved problem.
  
  
  OBJECTIVE
  Our aim was to reduce remote lung damage after lower limb IR by means of postconditioning.
  
  
  METHODS
  Male Wistar rats were divided into three groups: Sham-operated, IR, and Postconditioned (PostC). In the latter two groups rats underwent 180 min of exclusion of the infrarenal aorta. The reperfusion time was 4 h. Serum-free radical levels, tumor necrosis factor-α and interleukin-6 concentrations, histologic changes in the lung, wet/dry-ratio, myeloperoxidase activity, heat shock protein 72 level and blood gas changes were investigated.
  
  
  RESULTS
  Postconditioning reduced histological damage in the lung (P < 0.05). Free radical levels and tumor necrosis factor-α concentrations were significantly lower in the PostC group than in the IR group (P < 0.05 and P < 0.01, respectively). Interleukin-6 concentrations did not significantly differ in the PostC group. Compared with the IR group, lung myeloperoxidase activity was lower in the PostC group. Decreased pulmonary heat shock protein 72 level was observed in the PostC group compared with the IR group and the wet/dry-ratio was also significantly lower in the PostC group (P < 0.05). A noticeably higher arterial pO2 level was manifest in the PostC group after 2 and 4 h of reperfusion (P < 0.05).
  
  
  CONCLUSIONS
  Postconditioning reduced lung damage under experimental conditions, in the early period of reperfusion after lower limb IR injury.
authors.affiliation: 1(st) Department of Surgery, Semmelweis University, Budapest, Hungary.
authors.forename: David
authors.initials: D
authors.lastname: Garbaisz
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Turoczi
authors.forename: Andras
authors.initials: A
authors.lastname: Fulop
authors.forename: Oliver
authors.initials: O
authors.lastname: Rosero
authors.forename: Peter
authors.initials: P
authors.lastname: Aranyi
authors.forename: Peter
authors.initials: P
authors.lastname: Onody
authors.forename: Gabor
authors.initials: G
authors.lastname: Lotz
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Rakonczay
authors.forename: Zsolt
authors.initials: Z
authors.lastname: Balla
authors.forename: Laszlo
authors.initials: L
authors.lastname: Harsanyi
authors.forename: Attila
authors.initials: A
authors.lastname: Szijarto
mesh_terms: D055371:Acute Lung Injury / Q000209:etiology / Q000378:metabolism / Q000628:therapy*; D000818:Animals; D001012:Aorta, Abdominal / Q000601:surgery; D004195:Disease Models, Animal; D005609:Free Radicals / Q000378:metabolism; D006614:Hindlimb / Q000098:blood supply / Q000601:surgery; D015850:Interleukin-6 / Q000378:metabolism; D057775:Ischemic Postconditioning / Q000379:methods*; D008297:Male; D011183:Postoperative Complications / Q000209:etiology / Q000378:metabolism / Q000628:therapy*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000150:complications* / Q000378:metabolism; D013525:Surgical Instruments; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014656:Vascular Surgical Procedures / Q000009:adverse effects*
chemical_list: D005609:Free Radicals; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha
keywords: ARDS; Ischemia-reperfusion; Lower limb; Lung damage; Postconditioning; Systemic inflammatory response syndrome