title: Effect of bisphenol A on rat metabolic profiling studied by using capillary electrophoresis time-of-flight mass spectrometry.
abstract: Bisphenol A (BPA), a chemical widely used in the manufacture of polycarbonate plastics, has raised considerable concern in recent decades because of its hormone-like properties. Whether BPA exposure is a health risk remains controversial in many countries. A metabolomics study based on capillary electrophoresis time-of-flight mass spectrometry (CE-TOF/MS) was performed to study the urine metabolic profiles of Sprague-Dawley rats fed with four dose levels of BPA (0, 1, 10, and 100 μg/kg body weight) for 45 days. Multivariate pattern recognition directly reflected the metabolic perturbations caused by BPA. On the basis of univariate analysis, 42 metabolites including amino acids, polyamines, nucleosides, organic acids, carbohydrates, pterins, polyphenols, and sugar phosphates were found as the most significantly differential metabolites. The marked perturbations were related with valine, leucine and isoleucine biosynthesis, D-glutamine and D-glutamate metabolism, etc. Significant alterations of neurotransmitters (glutamate, gamma-aminobutyric acid, and noradrenaline) and neurotransmitter-related metabolites (tyrosine, histamine, valine, and taurine) suggested that the toxic effects of small-dose BPA (below 50 mg/kg/day) may contribute to its interactions with the neuromediating system. Our study demonstrated that metabolomics may offer more specific insights into the molecular changes underlying the physiological effects of BPA.
authors.affiliation: CAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zeng
authors.forename: Hua
authors.initials: H
authors.lastname: Kuang
authors.forename: Chunxiu
authors.initials: C
authors.lastname: Hu
authors.forename: Xianzhe
authors.initials: X
authors.lastname: Shi
authors.forename: Min
authors.initials: M
authors.lastname: Yan
authors.forename: Liguang
authors.initials: L
authors.lastname: Xu
authors.forename: Libing
authors.initials: L
authors.lastname: Wang
authors.forename: Chuanlai
authors.initials: C
authors.lastname: Xu
authors.forename: Guowang
authors.initials: G
authors.lastname: Xu
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000493:pharmacokinetics* / Q000652:urine; D019075:Electrophoresis, Capillary; D052244:Endocrine Disruptors / Q000493:pharmacokinetics* / Q000652:urine; D004968:Estrogens, Non-Steroidal / Q000493:pharmacokinetics* / Q000652:urine; D018698:Glutamic Acid / Q000378:metabolism; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D055442:Metabolome; D018377:Neurotransmitter Agents / Q000378:metabolism; D009638:Norepinephrine / Q000378:metabolism; D010636:Phenols / Q000493:pharmacokinetics* / Q000652:urine; D051381:Rats; D017207:Rats, Sprague-Dawley; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D004968:Estrogens, Non-Steroidal; D018698:Glutamic Acid; D018377:Neurotransmitter Agents; D009638:Norepinephrine; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds