title: Amperometric detection of dopamine in human serum by electrochemical sensor based on gold nanoparticles doped molecularly imprinted polymers.
abstract: In this work, a highly sensitive and selective biomimetic electrochemical sensor for the amperometric detection of trace dopamine (DA) in human serums was achieved by gold nanoparticles (AuNPs) doped molecularly imprinted polymers (MIPs). Functionalized AuNPs (F-AuNPs), a novel functional monomer bearing aniline moieties on the surface of the AuNPs, were prepared via a direct synthesis method and then used to fabricate the conductive MIPs film on the modified electrode by electropolymerization method in the presence of DA and p-aminobenzenethiol (p-ATP). The obtained electrochemical sensor based on the conductive film of AuNPs doped MIPs (AuNPs@MIPs) could effectively minimize the interferences caused by ascorbic acid (AA) and uric acid (UA). The linear range for amperometric detection of DA was from 0.02 μmol L(-1) to 0.54 μmol L(-1) with the detection limit of 7.8 nmol L(-1) (S/N=3). Furthermore, the AuNPs@MIPs modified electrode (AuNPs@MIES) was successfully employed to detect trace DA in different human serums.
authors.affiliation: School of Pharmacy, Nanjing Medical University, Nanjing 211166, PR China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Xue
authors.forename: Qing
authors.initials: Q
authors.lastname: Han
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.forename: Jinhua
authors.initials: J
authors.lastname: Wu
authors.forename: Tingting
authors.initials: T
authors.lastname: Wen
authors.forename: Ruoyu
authors.initials: R
authors.lastname: Wang
authors.forename: Junli
authors.initials: J
authors.lastname: Hong
authors.forename: Xuemin
authors.initials: X
authors.lastname: Zhou
authors.forename: Huijun
authors.initials: H
authors.lastname: Jiang
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry; D004298:Dopamine / Q000097:blood*; D015259:Dopamine Agents / Q000097:blood*; D055664:Electrochemical Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D054802:Molecular Imprinting; D053758:Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry
chemical_list: D000814:Aniline Compounds; D015259:Dopamine Agents; D011108:Polymers; D006046:Gold; C023650:aniline; D004298:Dopamine
keywords: Dopamine; Doping; Electrochemical sensor; Functionalized AuNPs; Molecularly imprinted polymers