title: Identification of phasiRNAs in wild rice (Oryza rufipogon).
abstract: Plant miRNAs can trigger the production of phased, secondary siRNAs from either non-coding or protein-coding genes. In this study, at least 864 and 3,961 loci generating 21-nt and 24-nt phased siRNAs (phasiRNAs),respectively, were identified in three tissues from wild rice. Of these phasiRNA-producing loci, or PHAS genes, biogenesis of phasiRNAs in at least 160 of 21-nt and 254 of 24-nt loci could be triggered by interaction with miRNA(s). Developing seeds had more PHAS genes than leaves and roots. Genetic constrain on miRNA-triggered PHAS genes suggests that phasiRNAs might be one of the driving forces contributed to rice domestication.
authors.affiliation: Department of Agronomy & James D. Watson Institute of Genome Sciences; Zhejiang University; Hangzhou, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.forename: Qian-Hao
authors.initials: QH
authors.lastname: Zhu
authors.forename: Longjiang
authors.initials: L
authors.lastname: Fan
mesh_terms: D001483:Base Sequence; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D056426:Genetic Loci / Q000235:genetics; D014644:Genetic Variation; D008969:Molecular Sequence Data; D012275:Oryza / Q000235:genetics* / Q000254:growth & development; D034741:RNA, Small Interfering / Q000096:biosynthesis / Q000235:genetics* / Q000302:isolation & purification; D012639:Seeds / Q000235:genetics / Q000254:growth & development
chemical_list: D034741:RNA, Small Interfering
keywords: Oryza rufipogon; PHAS; TAS; miRNA; microRNA; phased siRNA; phased siRNAs (phasiRNA); phasiRNA; phasiRNA locus; rice domestication; siRNA; small RNA; small-interfering RNA; tasiRNA; tasiRNA locus; trans-acting siRNA