title: Increased levels of cell-free human placental lactogen mRNA at 28-32 gestational weeks in plasma of pregnant women with placenta previa and invasive placenta.
abstract: We compared the levels of cell-free human placental lactogen (hPL) messenger RNA (mRNA) in maternal plasma at 28 to 32 weeks of gestation between women with diagnosis of placenta previa or invasive placenta and women with an uneventful pregnancy. Sensitivity and specificity of hPL mRNA for the prediction of invasive placenta were further explored. Plasma hPL mRNA were quantified by real-time reverse-transcriptase polymerase chain reaction in women with placenta previa (n = 13), invasive placenta (n = 5), and normal pregnancies (n = 92). Median (range) hPL mRNA was significantly higher in women with placenta previa, 782 (10-2301) copies/mL of plasma, and in those with invasive placenta, 615 (522-2102) copies/mL of plasma, when compared to normal pregnancies, 90 (4-4407) copies/mL of plasma, P < .01 and P < .05, respectively. We found a sensitivity of 100% and a specificity of 61.5% for the prediction of invasive placenta among women with placenta previa. In conclusion, expression of hPL mRNA is increased in plasma of women with placenta previa and invasive placenta at 28 to 32 weeks of gestation.
authors.affiliation: 1Department of Obstetrics and Gynecology, Self-Defense Forces Central Hospital, Tokyo, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Kawashima
authors.forename: Akihiko
authors.initials: A
authors.lastname: Sekizawa
authors.forename: Walter
authors.initials: W
authors.lastname: Ventura
authors.forename: Keiko
authors.initials: K
authors.lastname: Koide
authors.forename: Kyouko
authors.initials: K
authors.lastname: Hori
authors.forename: Takashi
authors.initials: T
authors.lastname: Okai
authors.forename: Yoshida
authors.initials: Y
authors.lastname: Masashi
authors.forename: Kenichi
authors.initials: K
authors.lastname: Furuya
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Mizumoto
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D002474:Cell-Free System; D005260:Female; D006801:Humans; D010920:Placenta / Q000378:metabolism* / Q000473:pathology; D010923:Placenta Previa / Q000097:blood* / Q000175:diagnosis*; D010928:Placental Lactogen / Q000097:blood*; D011247:Pregnancy / Q000097:blood*; D012333:RNA, Messenger / Q000097:blood*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D012333:RNA, Messenger; D010928:Placental Lactogen
keywords: cell-free RNA; hPL; placenta accreta; placenta previa