title: Defining skeletal muscle resident progenitors and their cell fate potentials.
abstract: The satellite cell is the major tissue-resident stem cell underlying muscle regeneration; however, multiple non-satellite myogenic progenitors as well as non-myogenic populations that support the muscle regenerative process have been identified. PW1 is expressed in satellite cells as well as in a subset of interstitial cells with myogenic potential termed PICs (PW1+ interstitial cells). Microarray profiling revealed that PICs express a broad range of genes common to mesenchymal stem cells, whereas satellite cells express genes consistent with a committed myogenic progenitor. Isolated PICs from both young and adult muscles can differentiate into smooth and skeletal muscle and fat whereas satellite cells are restricted to a skeletal muscle fate. We demonstrate that the adipogenic potential of PICs corresponds to a subpopulation that expresses platelet derived growth factor receptor alpha (PDGFRα) and overlaps with the recently described interstitial adipogenic progenitors. By contrast, PICs with myogenic potential do not express PDGFRα. Moreover, we observe a discrete and transient population of juvenile PICs based upon SCA1 expression that disappears by 3 weeks of postnatal development coincident with a switch in the cellular and genetic mechanisms underlying postnatal muscle growth.
authors.affiliation: Myology Group, UMR S 787 INSERM, University of Pierre and Marie Curie Paris VI, Paris 75634, France.
authors.forename: Alice
authors.initials: A
authors.lastname: Pannérec
authors.forename: Luigi
authors.initials: L
authors.lastname: Formicola
authors.forename: Vanessa
authors.initials: V
authors.lastname: Besson
authors.forename: Giovanna
authors.initials: G
authors.lastname: Marazzi
authors.forename: David A
authors.initials: DA
authors.lastname: Sassoon
mesh_terms: D050156:Adipogenesis; D000818:Animals; D002478:Cells, Cultured; D004622:Embryo, Mammalian / Q000166:cytology / Q000378:metabolism; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018482:Muscle, Skeletal / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism; D032496:Satellite Cells, Skeletal Muscle / Q000166:cytology* / Q000378:metabolism; D059467:Transcriptome
chemical_list: D051741:Kruppel-Like Transcription Factors; C097901:Peg3 protein, mouse
keywords: Mouse; PDGFRα; PW1; Skeletal muscle; Stem cell