title: Development of a new, simple and cost-effective diagnostic tool for genetic screening of hereditary colorectal cancer--the DNA microarray assay.
abstract: Detection of mutations in families with a hereditary predisposition to colon cancer gives an opportunity to precisely define the high-risk group. 36 patients operated on for colon cancer, with familiar prevalence of this malignancy, were investigated using the DNA microarrays method with the potential detection of 170 mutations in MLH1, MSH2, MSH6, CHEK2, and NOD2 genes. In microarrays analysis of DNA in 9 patients (25% of the investigated group), 6 different mutations were found. The effectiveness of genetic screening using the microarray method is comparable to the effectiveness of other, much more expensive and time-consuming methods.
authors.affiliation: Regional Specialistic Hospital, Słupsk, Department of General, Vascular and Oncologic, Surgery, Słupsk, Poland.
authors.forename: Zoran
authors.initials: Z
authors.lastname: Stojcev
authors.forename: Tomasz
authors.initials: T
authors.lastname: Banasiewicz
authors.forename: Michał
authors.initials: M
authors.lastname: Kaszuba
authors.forename: Adam
authors.initials: A
authors.lastname: Sikorski
authors.forename: Marek
authors.initials: M
authors.lastname: Szczepkowski
authors.forename: Adam
authors.initials: A
authors.lastname: Bobkiewicz
authors.forename: Jacek
authors.initials: J
authors.lastname: Paszkowski
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Krokowicz
authors.forename: Maciej
authors.initials: M
authors.lastname: Biczysko
authors.forename: Jacek
authors.initials: J
authors.lastname: Szmeja
authors.forename: Monika
authors.initials: M
authors.lastname: Jurkowska
authors.forename: Przemysław
authors.initials: P
authors.lastname: Majewski
authors.forename: Andrzej
authors.initials: A
authors.lastname: Mackiewicz
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Lamperska
authors.forename: Michał
authors.initials: M
authors.lastname: Drews
authors.forename: Jacek
authors.initials: J
authors.lastname: Wojciechowicz
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D003123:Colorectal Neoplasms, Hereditary Nonpolyposis / Q000175:diagnosis* / Q000235:genetics*; D053843:DNA Mismatch Repair / Q000235:genetics*; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D000070957:MutL Protein Homolog 1; D051718:MutS Homolog 2 Protein / Q000235:genetics; D009687:Nuclear Proteins; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010865:Pilot Projects
chemical_list: D048868:Adaptor Proteins, Signal Transducing; C501486:MLH1 protein, human; D009687:Nuclear Proteins; C497172:MSH2 protein, human; D000070957:MutL Protein Homolog 1; D051718:MutS Homolog 2 Protein