title: PCR-based molecular characterization of Toxocara spp. using feces of stray cats: a study from Southwest Iran.
abstract: Feces of stray cat are potential sources of gastrointestinal parasites and play a crucial role in spreading and transmitting parasite eggs, larvae, and oocysts through contamination of soil, food, or water. In this study, we investigated the prevalence of Toxocara spp. infection in stray cats in Ahvaz city, southwest Iran. Eggs of Toxocara spp. in feces of stray cats were detected by the sucrose flotation method, and identification was conducted by polymerase chain reaction (PCR) and DNA sequencing. Of the 140 fecal samples that were randomly collected from public environments during the months of January to May 2012, 45% were found to harbour Toxocara spp. eggs. The highest prevalence of Toxocara spp. eggs was found in the central area of Ahvaz city (28.6%). T. canis eggs were found in 4 (6.34%) of the 63 positive samples. Stray cats are found in parks, playgrounds, and other public places and may be a potential contamination risk. Identification of Toxocara spp. using molecular methods is sufficiently sensitive to detect low levels of parasites and identify the different Toxocara spp. in feces. The relatively high prevalence of Toxocara spp. infection may continue to increase due to lack of effective environmental hygiene control in Iran. Consequently, there is a need to plan adequate programs to detect, identify, and control this infection as well as stray cats in the region.
authors.affiliation: Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. khademvatan@yahoo.com
authors.forename: Shahram
authors.initials: S
authors.lastname: Khademvatan
authors.forename: Fakher
authors.initials: F
authors.lastname: Rahim
authors.forename: Mahdi
authors.initials: M
authors.lastname: Tavalla
authors.forename: Rahman
authors.initials: R
authors.lastname: Abdizadeh
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Hashemitabar
mesh_terms: D000818:Animals; D001483:Base Sequence; D002371:Cat Diseases / Q000453:epidemiology* / Q000469:parasitology; D002415:Cats; D002947:Cities; D018509:DNA, Helminth / Q000235:genetics*; D005243:Feces / Q000469:parasitology; D007492:Iran / Q000453:epidemiology; D008969:Molecular Sequence Data; D010270:Parasite Egg Count; D016133:Polymerase Chain Reaction; D014119:Toxocara / Q000235:genetics / Q000302:isolation & purification*; D014120:Toxocariasis / Q000453:epidemiology* / Q000469:parasitology
chemical_list: D018509:DNA, Helminth