title: An aptasensor for electrochemical detection of tumor necrosis factor in human blood.
abstract: Electrochemical aptasensors can detect disease markers such as cytokines to provide point-of-care diagnosis that is low-cost, rapid, specific and sensitive. Herein, we describe the development of an aptamer-based electrochemical sensor for detection and analysis of tumor necrosis factor-alpha (TNF-α) - a key inflammatory cytokine - in whole human blood. When testing spiked blood, a TNF-α detection limit of 58 pM (10 ng mL(-1)) and a linear range of 6 nM (100 ng mL(-1)) could be achieved. Furthermore, detection of TNF-α in mitogen stimulated whole blood was demonstrated.
authors.affiliation: Department of Biomedical Engineering, University of California, 451 Health Sciences Drive, #2519, Davis, CA 95616, USA. ucyliu@ucdavis.edu
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhou
authors.forename: Alexander
authors.initials: A
authors.lastname: Revzin
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D055664:Electrochemical Techniques / Q000379:methods*; D006801:Humans; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*
chemical_list: D052157:Aptamers, Nucleotide; D014409:Tumor Necrosis Factor-alpha