title: Comparing gene expression profiles of Kashin-Beck and Keshan diseases occurring within the same endemic areas of China.
abstract: In this study, differentially expressed genes in peripheral blood from patients with Kashin-Beck disease and Keshan disease were compared to further investigate the etiology and pathogenesis of both diseases, which occur in a common endemic area of China. Twenty Kashin-Beck disease patients and 12 healthy controls, and 16 Keshan disease patients and 16 healthy controls, were grouped into four pairs. Patients and controls were selected from common endemic areas for the two diseases. Total RNA was isolated from peripheral blood mononuclear cells from all patients and controls, and gene expression profiles analyzed by oligonucleotide microarrays. Sixteen genes differentially expressed in both Kashin-Beck disease and Keshan disease (versus controls) were identified, and comprised nine genes showing synchronous and seven asynchronous expression. The Comparative Toxicogenomics Database shows that expression and biological function of these genes can be affected by multiple environmental factors, including mycotoxin and selenium content, potential environmental risk factors for the two diseases. Thus, these shared differentially expressed genes may contribute to the distinct organ lesions, caused by common environmental risk factors of Kashin-Beck disease and Keshan disease.
authors.affiliation: Faculty of Public Health, Medical College of Xi'an Jiaotong University, Key Laboratory of Environment and Gene Related Diseases of Ministry of Education, Key Laboratory of Trace Elements and Endemic Diseases of Ministry of Health, Xi'an 710061, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Wang
authors.forename: Shuang
authors.initials: S
authors.lastname: Wang
authors.forename: Shulan
authors.initials: S
authors.lastname: He
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.forename: Wuhong
authors.initials: W
authors.lastname: Tan
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Lei
authors.forename: Hanjie
authors.initials: H
authors.lastname: Yu
authors.forename: Zheng
authors.initials: Z
authors.lastname: Li
authors.forename: Yujie
authors.initials: Y
authors.lastname: Ning
authors.forename: Youzhang
authors.initials: Y
authors.lastname: Xiang
authors.forename: Xiong
authors.initials: X
authors.lastname: Guo
mesh_terms: D000328:Adult; D000368:Aged; D009202:Cardiomyopathies / Q000453:epidemiology / Q000235:genetics*; D016022:Case-Control Studies; D002681:China / Q000453:epidemiology; D019353:Endemic Diseases; D004769:Enterovirus Infections / Q000453:epidemiology / Q000235:genetics*; D020869:Gene Expression Profiling; D006801:Humans; D057767:Kashin-Beck Disease / Q000453:epidemiology / Q000235:genetics*; D008875:Middle Aged; D060888:Real-Time Polymerase Chain Reaction