title: Generation of human epidermis-derived mesenchymal stem cell-like pluripotent cells (hEMSCPCs).
abstract: We isolated human epidermis-derived mesenchymal stem cell-like pluripotent cells (hEMSCPCs) and demonstrate efficient harvesting, maintenance in vitro for at least 30 passages, reprogramming into multiple phenotypes in vivo, and integration into adult host tissues after injection into the mouse blastocyst to create chimeras. Cell phenotype was examined by karyotyping, immunostaining, immunofluorescence, and flow cytometry. A nested PCR protocol using primers specific for human SRY genes was designed to detect hEMSCPC-derived cells in female chimeric mice. FISH was used to validate the results of nested PCR. Results indicated that hEMSCPCs were derived from epidermis but were distinct from epidermal cells; they resembled mesenchymal stem cells (MSCs) morphologically and expressed the main markers of MSCs. About half of all female offspring of mice implanted with embryos injected with hEMSCPCs at the blastocyst stage harbored the human Y chromosome and tissue-specific human protein, thereby demonstrating the transdifferentiation of hEMSCPCs.
authors.affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, GuangZhou 510060, China. huangbing2000@hotmail.com
authors.forename: Bing
authors.initials: B
authors.lastname: Huang
authors.forename: Kaijing
authors.initials: K
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Yu
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.forename: Yongping
authors.initials: Y
authors.lastname: Li
authors.forename: Weihua
authors.initials: W
authors.lastname: Li
authors.forename: Wencong
authors.initials: W
authors.lastname: Wang
authors.forename: Liping
authors.initials: L
authors.lastname: Guan
authors.forename: Wenxin
authors.initials: W
authors.lastname: Zhang
authors.forename: Shaochun
authors.initials: S
authors.lastname: Lin
authors.forename: Xintao
authors.initials: X
authors.lastname: Huang
authors.forename: Liping
authors.initials: L
authors.lastname: Lin
authors.forename: Yongliang
authors.initials: Y
authors.lastname: Lin
authors.forename: Yichi
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xinming
authors.initials: X
authors.lastname: Song
authors.forename: Zhichong
authors.initials: Z
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Ge
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D001755:Blastocyst / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation; D004622:Embryo, Mammalian / Q000166:cytology* / Q000378:metabolism; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism; D005260:Female; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D007124:Immunoenzyme Techniques; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D051876:Sex-Determining Region Y Protein / Q000235:genetics
chemical_list: D015415:Biomarkers; C497745:SRY protein, human; D051876:Sex-Determining Region Y Protein