title: Imaging of jasmonic acid binding sites in tissue.
abstract: Hormones regulate the mechanism of plant growth and development, senescence, and plants' adaptation to the environment; studies of the molecular mechanisms of plant hormone action are necessary for the understanding of these complex phenomena. However, there is no measurable signal for the hormone signal transduction process. We synthesized and applied a quantum dot-based fluorescent probe for the labeling of jasmonic acid (JA) binding sites in plants. This labeling probe was obtained by coupling mercaptoethylamine-modified CdTe quantum dots with JA using N-hydroxysuccinimide (NHS) as a coupling agent. The probe, CdTe-JA, was characterized by transmission electron microscopy, dynamic light scattering, and fluorescent spectrum and applied in labeling JA binding sites in tissue sections of mung bean seedlings and Arabidopsis thaliana root tips. Laser scanning confocal microscopy (LSCM) revealed that the probe selectively labeled JA receptor. The competition assays demonstrated that the CdTe-JA probe retained the original bioactivity of JA. An LSCM three-dimensional reconstruction experiment demonstrated excellent photostability of the probe.
authors.affiliation: School of Chemistry and Environment, South China Normal University, Guangzhou 510006, People's Republic of China.
authors.forename: Yan hong
authors.initials: Yh
authors.lastname: Gao
authors.forename: Ying
authors.initials: Y
authors.lastname: Yu
authors.forename: Xiao gang
authors.initials: Xg
authors.lastname: Hu
authors.forename: Yu juan
authors.initials: Yj
authors.lastname: Cao
authors.forename: Jian zhong
authors.initials: Jz
authors.lastname: Wu
mesh_terms: D017360:Arabidopsis / Q000166:cytology; D001665:Binding Sites; D019187:Cadmium Compounds / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism*; D003543:Cysteamine / Q000378:metabolism; D021621:Imaging, Three-Dimensional; D057054:Molecular Imaging / Q000379:methods*; D054883:Oxylipins / Q000378:metabolism*; D045663:Quantum Dots; D036226:Seedlings / Q000166:cytology; D013050:Spectrometry, Fluorescence; D013691:Tellurium / Q000378:metabolism
chemical_list: D019187:Cadmium Compounds; D003517:Cyclopentanes; D054883:Oxylipins; D003543:Cysteamine; C011006:jasmonic acid; D013691:Tellurium; C028337:cadmium telluride
keywords: Binding sites; CdTe QDs; Fluorescent probe; Jasmonic acid