title: Distinct populations of adipogenic and myogenic Myf5-lineage progenitors in white adipose tissues.
abstract: Brown adipose tissues (BAT) are derived from a myogenic factor 5 (Myf5)-expressing cell lineage and white adipose tissues (WAT) predominantly arise from non-Myf5 lineages, although a subpopulation of adipocytes in some WAT depots can be derived from the Myf5 lineage. However, the functional implication of the Myf5- and non-Myf5-lineage cells in WAT is unclear. We found that the Myf5-lineage constitution in subcutaneous WAT depots is negatively correlated to the expression of classical BAT and newly defined beige/brite adipocyte-specific genes. Consistently, fluorescent-activated cell sorting (FACS)-purified Myf5-lineage adipo-progenitors give rise to adipocytes expressing lower levels of BAT-specific Ucp1, Prdm16, Cidea, and Ppargc1a genes and beige adipocyte-specific CD137, Tmem26, and Tbx1 genes compared with the non-Myf5-lineage adipocytes from the same depots. Ablation of the Myf5-lineage progenitors in WAT stromal vascular cell (SVC) cultures leads to increased expression of BAT and beige cell signature genes. Strikingly, the Myf5-lineage cells in WAT are heterogeneous and contain distinct adipogenic [stem cell antigen 1(Sca1)-positive] and myogenic (Sca1-negative) progenitors. The latter differentiate robustly into myofibers in vitro and in vivo, and they restore dystrophin expression after transplantation into mdx mouse, a model for Duchenne muscular dystrophy. These results demonstrate the heterogeneity and functional differences of the Myf5- and non-Myf5-lineage cells in the white adipose tissue.
authors.affiliation: Department of Animal Science and Purdue University, West Lafayette, IN 47907.
authors.forename: Tizhong
authors.initials: T
authors.lastname: Shan
authors.affiliation: Department of Animal Science and Purdue University, West Lafayette, IN 47907.
authors.forename: Xinrong
authors.initials: X
authors.lastname: Liang
authors.affiliation: Department of Animal Science and Purdue University, West Lafayette, IN 47907.
authors.forename: Pengpeng
authors.initials: P
authors.lastname: Bi
authors.affiliation: Department of Animal Science and Purdue University, West Lafayette, IN 47907.
authors.forename: Pengpeng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Department of Animal Science and Purdue University, West Lafayette, IN 47907.
authors.forename: Weiyi
authors.initials: W
authors.lastname: Liu
authors.affiliation: Department of Animal Science and Purdue University, West Lafayette, IN 47907; Purdue University Center for Cancer Research, Purdue University, West Lafayette, IN 47907. Electronic address: skuang@purdue.edu.
authors.forename: Shihuan
authors.initials: S
authors.lastname: Kuang
mesh_terms: D017667:Adipocytes / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D052436:Adipose Tissue, White / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D000818:Animals; D002478:Cells, Cultured; D051379:Mice; D018101:Mice, Inbred mdx; D032446:Myoblasts / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D051787:Myogenic Regulatory Factor 5 / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: C497418:Myf5 protein, mouse; D051787:Myogenic Regulatory Factor 5
keywords: Cre/LoxP; brown adipose tissue; diabetes; lineage tracing; progenitor cell; regeneration