title: UV-induced Nucleotide Excision Repair (NER) and Photoreactivation Repair (PER) in two trout fish cell lines used in ecotoxicological assessment studies.
abstract: A better knowledge of DNA repair capacities in permanent fish cell lines would contribute to establish their interest in genotoxicity testing for environmental risk assessment studies including the effects of an increase in solar UV radiations on aquatic organisms. NER (Nucleotide Excision Repair) and PER (Photoreactivation Repair) are the two repair pathways of choice for UV-induced photo-lesions. In the present paper, these repair processes were characterized in the two rainbow trout cell lines, RTGill-W1 and RTL-W1 (liver), by means of a T4-modified comet assay which allowed to follow the cyclobutane pyrimidine dimers repair kinetics specifically. Both repair processes have been found in the cell lines, PER repairing much faster UV lesions than NER, and NER being slightly more efficient in the gill cell line than in the liver one.
authors.affiliation: Université de Lyon, UMR LEHNA 5023, F-69100 Villeurbanne, France.
authors.forename: Aude
authors.initials: A
authors.lastname: Kienzler
authors.forename: Xavier
authors.initials: X
authors.lastname: Tronchère
authors.forename: Alain
authors.initials: A
authors.lastname: Devaux
authors.forename: Sylvie
authors.initials: S
authors.lastname: Bony
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000528:radiation effects; D020552:Comet Assay; D004249:DNA Damage; D004260:DNA Repair; D014337:Trout; D014466:Ultraviolet Rays / Q000009:adverse effects*
keywords: DNA repair; Fish cell line; Genotoxicity; UV