title: Allogeneic lethally irradiated cord blood mononuclear cells in no-option critical limb ischemia: a "box of rain".
abstract: Critical limb ischemia (CLI) is burdened by a 40% major amputation rate, and a 5-year life expectancy <50%. We report the first in-human injection of lethally γ-irradiated non-human leukocyte antigen (HLA)-matched cord blood (CB)-derived mononuclear cells in a no-option CLI patient, to induce therapeutic neo-angiogenesis, with evidence of successful outcome supported by clinical findings (ulcer healing and pain relief), instrumental assessment (transcutaneous O2 pressure, ankle/brachial index, and contrast-enhanced ultrasonography), and histological demonstration of muscular tissue repair and capillary network expansion. If our approach will be confirmed, the huge number of CB units currently discarded might be redirected toward regenerative medicine purposes, leading to cutting-edge solutions for important unmet clinical needs, such as ischemic diseases, which remain the main cause of disability and mortality in western countries.
authors.affiliation: 1 Immunohematology and Transfusion Service, IRCCS Policlinico San Matteo of Pavia Foundation , Pavia, Italy .
authors.forename: Cesare
authors.initials: C
authors.lastname: Perotti
authors.forename: Vittorio
authors.initials: V
authors.lastname: Arici
authors.forename: Marila
authors.initials: M
authors.lastname: Cervio
authors.forename: Claudia
authors.initials: C
authors.lastname: Del Fante
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Calliada
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Gnecchi
authors.forename: Maria Chiara
authors.initials: MC
authors.lastname: Ciuffreda
authors.forename: Luigia
authors.initials: L
authors.lastname: Scudeller
authors.forename: Antonio
authors.initials: A
authors.lastname: Bozzani
authors.forename: Franco
authors.initials: F
authors.lastname: Ragni
authors.forename: Gianluca
authors.initials: G
authors.lastname: Viarengo
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Cervio
authors.forename: Attilio
authors.initials: A
authors.lastname: Odero
authors.forename: Carlo Alberto
authors.initials: CA
authors.lastname: Redi
mesh_terms: D000368:Aged; D057176:Compassionate Use Trials; D005312:Fetal Blood / Q000166:cytology*; D005528:Foot / Q000098:blood supply / Q000473:pathology*; D016523:Foot Ulcer / Q000000981:diagnostic imaging / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D005720:Gamma Rays; D006085:Graft Survival / Q000276:immunology*; D006680:HLA Antigens / Q000276:immunology; D006801:Humans; D007511:Ischemia / Q000000981:diagnostic imaging / Q000276:immunology / Q000473:pathology / Q000628:therapy*; D007963:Leukocytes, Mononuclear / Q000166:cytology* / Q000528:radiation effects / Q000637:transplantation; D008297:Male; D018919:Neovascularization, Physiologic; D020127:Recovery of Function; D014184:Transplantation, Homologous; D016896:Treatment Outcome; D014463:Ultrasonography
chemical_list: D006680:HLA Antigens