title: Parallel on-axis phase-shifting holographic phase microscopy based on reflective point-diffraction interferometer with long-term stability.
abstract: Parallel on-axis two-step phase-shifting reflective point-diffraction interferometry for holographic phase microscopy based on Michelson architecture is proposed. A cube beamsplitter splits the object wave into two copies within the two arms. The reference wave is rebuilt by low-pass filtering with a pinhole-masked mirror. Both object and reference waves are split into two beams by a grating in a 4f imaging system; thus, two interferograms with quadrature phase-shift can be acquired simultaneously with the aid of polarization elements. The approach has the merit of nanometers-scale phase stability over hours due to its quasi-common-path geometry. It can make full use of camera spatial bandwidth while its temporal resolution is as fast as the camera frame rate. Phase imaging on microscale specimen is implemented, and the experimental results demonstrate that the proposed approach is suitable for investigating dynamic processes.
authors.affiliation: State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an, China.
authors.forename: Rongli
authors.initials: R
authors.lastname: Guo
authors.forename: Baoli
authors.initials: B
authors.lastname: Yao
authors.forename: Peng
authors.initials: P
authors.lastname: Gao
authors.forename: Junwei
authors.initials: J
authors.lastname: Min
authors.forename: Jun
authors.initials: J
authors.lastname: Han
authors.forename: Xun
authors.initials: X
authors.lastname: Yu
authors.forename: Ming
authors.initials: M
authors.lastname: Lei
authors.forename: Shaohui
authors.initials: S
authors.lastname: Yan
authors.forename: Yanlong
authors.initials: Y
authors.lastname: Yang
authors.forename: Dan
authors.initials: D
authors.lastname: Dan
authors.forename: Tong
authors.initials: T
authors.lastname: Ye
mesh_terms: D003952:Diagnostic Imaging / Q000379:methods; D004867:Equipment Design; D004912:Erythrocytes / Q000473:pathology; D005583:Fourier Analysis; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D007368:Interferometry / Q000295:instrumentation* / Q000379:methods*; D008853:Microscopy / Q000379:methods; D019904:Polymethyl Methacrylate / Q000737:chemistry; D012031:Refractometry; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry; D014867:Water
chemical_list: D014867:Water; D012822:Silicon Dioxide; D019904:Polymethyl Methacrylate