title: Mapping Novel Pathways in Cardiovascular Disease Using eQTL Data: The Past, Present, and Future of Gene Expression Analysis.
abstract: Genome-wide association studies (GWAS) have identified genetic variants associated with numerous cardiovascular and metabolic diseases. Newly identified polymorphisms associated with myocardial infarction, dyslipidemia, hypertension, diabetes, and insulin resistance suggest novel mechanistic pathways that underlie these and other complex diseases. Working out the connections between the polymorphisms identified in GWAS and their biological mechanisms has been especially challenging given the number of non-coding variants identified thus far. In this review, we discuss the utility of expression quantitative trait locus (eQTL) databases in the study of non-coding variants with respect to cardiovascular and metabolic phenotypes. Recent successes in using eQTL data to link variants with functional candidate genes will be reviewed, and the shortcomings of this approach will be outlined. Finally, we discuss the emerging next generation of eQTL studies that take advantage of the ability to generate induced pluripotent stem cell lines from population cohorts.
authors.affiliation: Department of Stem Cell and Regenerative Biology, Harvard University Cambridge, MA, USA ; Division of Cardiovascular Medicine, Brigham and Women's Hospital Boston, MA, USA.
authors.forename: Rajat M
authors.initials: RM
authors.lastname: Gupta
authors.forename: Kiran
authors.initials: K
authors.lastname: Musunuru
keywords: GWAS; RNA-seq; cardiovascular disease; eQTL; induced pluripotent stem cells