title: Avidity of onconeural antibodies is of clinical relevance.
abstract: Onconeural antibodies are important in the detection of paraneoplastic neurological syndromes (PNS). The avidity of Hu, Yo, and CRMP5 antibodies from 100 patients was determined by immunoprecipitation (IP), and 13 of the Yo positive sera were also tested by surface plasmon resonance (SPR). There was a significant association between the results from IP and SPR. Yo antibodies had higher avidity than Hu and CRMP5 antibodies, and both high- and low-avidity antibodies were associated with tumors and PNS. High-avidity Yo antibodies were mainly associated with ovarian cancer, whereas high-avidity Hu and CRMP5 antibodies were mainly associated with small-cell lung cancer. Low-avidity CRMP5 and Yo antibodies were less often detected by a commercial line blot than high-avidity antibodies. The failure to detect low-avidity onconeural antibodies may result in under diagnosis of PNS.
authors.affiliation: Department of Neurology, Haukeland University Hospital, Jonas Lies vei 65, 5021, Bergen, Norway. cecilie.totland@helse-bergen.no
authors.forename: Cecilie
authors.initials: C
authors.lastname: Totland
authors.forename: Ming
authors.initials: M
authors.lastname: Ying
authors.forename: Mette
authors.initials: M
authors.lastname: Haugen
authors.forename: Kibret
authors.initials: K
authors.lastname: Mazengia
authors.forename: Anette
authors.initials: A
authors.lastname: Storstein
authors.forename: Jan
authors.initials: J
authors.lastname: Aarseth
authors.forename: Aurora
authors.initials: A
authors.lastname: Martinez
authors.forename: Christian
authors.initials: C
authors.lastname: Vedeler
mesh_terms: D000912:Antibodies, Neoplasm / Q000097:blood / Q000276:immunology*; D000915:Antibody Affinity; D051959:ELAV Proteins / Q000276:immunology; D006801:Humans; D006867:Hydrolases; D047468:Immunoprecipitation; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins / Q000276:immunology; D010257:Paraneoplastic Syndromes / Q000097:blood / Q000175:diagnosis* / Q000276:immunology*; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance
chemical_list: D000912:Antibodies, Neoplasm; C095184:CDR2 protein, human; D051959:ELAV Proteins; D008869:Microtubule-Associated Proteins; D009419:Nerve Tissue Proteins; C415087:DPYSL5 protein, human; D006867:Hydrolases